LOCUS       DAZBTF010000001        93591 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000001 DAZBTF010000000
VERSION     DAZBTF010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 93591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 93591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..93591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.001"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..281)
                     /locus_tag="ACHGNS_000001"
     CDS             complement(<1..281)
                     /locus_tag="ACHGNS_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFN6935989.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAP"
     gene            632..1264
                     /locus_tag="ACHGNS_000002"
     CDS             632..1264
                     /locus_tag="ACHGNS_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFN6935990.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            1387..2025
                     /locus_tag="ACHGNS_000003"
     CDS             1387..2025
                     /locus_tag="ACHGNS_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFN6935991.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(2144..2216)
                     /locus_tag="ACHGNS_000004"
     tRNA            complement(2144..2216)
                     /locus_tag="ACHGNS_000004"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2181..2183),aa:Thr,seq:cgt)
     gene            2337..2579
                     /locus_tag="ACHGNS_000005"
     CDS             2337..2579
                     /locus_tag="ACHGNS_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6935992.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            complement(2718..3347)
                     /gene="upp"
                     /locus_tag="ACHGNS_000006"
     CDS             complement(2718..3347)
                     /gene="upp"
                     /locus_tag="ACHGNS_000006"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFN6935993.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(3481..4428)
                     /locus_tag="ACHGNS_000007"
     CDS             complement(3481..4428)
                     /locus_tag="ACHGNS_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFN6935994.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            complement(4581..5825)
                     /gene="glyA"
                     /locus_tag="ACHGNS_000008"
     CDS             complement(4581..5825)
                     /gene="glyA"
                     /locus_tag="ACHGNS_000008"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFN6935995.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            complement(6016..7041)
                     /locus_tag="ACHGNS_000009"
     CDS             complement(6016..7041)
                     /locus_tag="ACHGNS_000009"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFN6935996.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            complement(7063..7242)
                     /locus_tag="ACHGNS_000010"
     CDS             complement(7063..7242)
                     /locus_tag="ACHGNS_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6935997.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            complement(7235..8074)
                     /gene="prmC"
                     /locus_tag="ACHGNS_000011"
     CDS             complement(7235..8074)
                     /gene="prmC"
                     /locus_tag="ACHGNS_000011"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFN6935998.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            complement(8067..9140)
                     /gene="prfA"
                     /locus_tag="ACHGNS_000012"
     CDS             complement(8067..9140)
                     /gene="prfA"
                     /locus_tag="ACHGNS_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFN6935999.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            complement(9143..9727)
                     /locus_tag="ACHGNS_000013"
     CDS             complement(9143..9727)
                     /locus_tag="ACHGNS_000013"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFN6936000.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            complement(9833..10081)
                     /locus_tag="ACHGNS_000014"
     CDS             complement(9833..10081)
                     /locus_tag="ACHGNS_000014"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6936001.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            complement(10072..10302)
                     /locus_tag="ACHGNS_000015"
     CDS             complement(10072..10302)
                     /locus_tag="ACHGNS_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936002.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            10408..11010
                     /locus_tag="ACHGNS_000016"
     CDS             10408..11010
                     /locus_tag="ACHGNS_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFN6936003.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            complement(11114..11683)
                     /gene="lepB"
                     /locus_tag="ACHGNS_000017"
     CDS             complement(11114..11683)
                     /gene="lepB"
                     /locus_tag="ACHGNS_000017"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFN6936004.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(11913..12680)
                     /locus_tag="ACHGNS_000018"
     CDS             complement(11913..12680)
                     /locus_tag="ACHGNS_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFN6936005.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            complement(12677..13156)
                     /locus_tag="ACHGNS_000019"
     CDS             complement(12677..13156)
                     /locus_tag="ACHGNS_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFN6936006.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            complement(13281..14018)
                     /locus_tag="ACHGNS_000020"
     CDS             complement(13281..14018)
                     /locus_tag="ACHGNS_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFN6936007.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            14179..15579
                     /locus_tag="ACHGNS_000021"
     CDS             14179..15579
                     /locus_tag="ACHGNS_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFN6936008.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            15802..17154
                     /locus_tag="ACHGNS_000022"
     CDS             15802..17154
                     /locus_tag="ACHGNS_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFN6936009.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            17147..17845
                     /locus_tag="ACHGNS_000023"
     CDS             17147..17845
                     /locus_tag="ACHGNS_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFN6936010.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(17907..19058)
                     /locus_tag="ACHGNS_000024"
     CDS             complement(17907..19058)
                     /locus_tag="ACHGNS_000024"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFN6936011.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(19071..19508)
                     /locus_tag="ACHGNS_000025"
     CDS             complement(19071..19508)
                     /locus_tag="ACHGNS_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN6936012.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(19523..21289)
                     /locus_tag="ACHGNS_000026"
     CDS             complement(19523..21289)
                     /locus_tag="ACHGNS_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFN6936013.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            21553..22230
                     /locus_tag="ACHGNS_000027"
     CDS             21553..22230
                     /locus_tag="ACHGNS_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFN6936014.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            22233..23360
                     /locus_tag="ACHGNS_000028"
     CDS             22233..23360
                     /locus_tag="ACHGNS_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6936015.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLLDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(23560..24000)
                     /locus_tag="ACHGNS_000029"
     CDS             complement(23560..24000)
                     /locus_tag="ACHGNS_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN6936016.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(24011..26044)
                     /locus_tag="ACHGNS_000030"
     CDS             complement(24011..26044)
                     /locus_tag="ACHGNS_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFN6936017.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(26046..27749)
                     /locus_tag="ACHGNS_000031"
     CDS             complement(26046..27749)
                     /locus_tag="ACHGNS_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN6936018.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(27843..28793)
                     /gene="manA"
                     /locus_tag="ACHGNS_000032"
     CDS             complement(27843..28793)
                     /gene="manA"
                     /locus_tag="ACHGNS_000032"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFN6936019.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            complement(28832..29857)
                     /locus_tag="ACHGNS_000033"
     CDS             complement(28832..29857)
                     /locus_tag="ACHGNS_000033"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFN6936020.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(30066..30935)
                     /locus_tag="ACHGNS_000034"
     CDS             complement(30066..30935)
                     /locus_tag="ACHGNS_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN6936021.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(31639..33531)
                     /locus_tag="ACHGNS_000035"
     CDS             complement(31639..33531)
                     /locus_tag="ACHGNS_000035"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6936022.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(33528..35255)
                     /locus_tag="ACHGNS_000036"
     CDS             complement(33528..35255)
                     /locus_tag="ACHGNS_000036"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6936023.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(35271..35921)
                     /locus_tag="ACHGNS_000037"
     CDS             complement(35271..35921)
                     /locus_tag="ACHGNS_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFN6936024.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            36125..36421
                     /locus_tag="ACHGNS_000038"
     CDS             36125..36421
                     /locus_tag="ACHGNS_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFN6936025.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            36613..37233
                     /locus_tag="ACHGNS_000039"
     CDS             36613..37233
                     /locus_tag="ACHGNS_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFN6936026.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(37325..38491)
                     /locus_tag="ACHGNS_000040"
     CDS             complement(37325..38491)
                     /locus_tag="ACHGNS_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFN6936027.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(38503..38856)
                     /locus_tag="ACHGNS_000041"
     CDS             complement(38503..38856)
                     /locus_tag="ACHGNS_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFN6936028.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            38984..40006
                     /locus_tag="ACHGNS_000042"
     CDS             38984..40006
                     /locus_tag="ACHGNS_000042"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFN6936029.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            40025..40186
                     /locus_tag="ACHGNS_000043"
     CDS             40025..40186
                     /locus_tag="ACHGNS_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936030.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(40445..40879)
                     /locus_tag="ACHGNS_000044"
     CDS             complement(40445..40879)
                     /locus_tag="ACHGNS_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936031.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(40989..42848)
                     /locus_tag="ACHGNS_000045"
     CDS             complement(40989..42848)
                     /locus_tag="ACHGNS_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFN6936032.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            42849..42989
                     /locus_tag="ACHGNS_000046"
     CDS             42849..42989
                     /locus_tag="ACHGNS_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFN6936033.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(42966..44126)
                     /locus_tag="ACHGNS_000047"
     CDS             complement(42966..44126)
                     /locus_tag="ACHGNS_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFN6936034.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(44433..45857)
                     /gene="araA"
                     /locus_tag="ACHGNS_000048"
     CDS             complement(44433..45857)
                     /gene="araA"
                     /locus_tag="ACHGNS_000048"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFN6936035.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(45873..46571)
                     /locus_tag="ACHGNS_000049"
     CDS             complement(45873..46571)
                     /locus_tag="ACHGNS_000049"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFN6936036.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(46528..48189)
                     /locus_tag="ACHGNS_000050"
     CDS             complement(46528..48189)
                     /locus_tag="ACHGNS_000050"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFN6936037.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            48407..49807
                     /locus_tag="ACHGNS_000051"
     CDS             48407..49807
                     /locus_tag="ACHGNS_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFN6936038.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(50059..51138)
                     /locus_tag="ACHGNS_000052"
     CDS             complement(50059..51138)
                     /locus_tag="ACHGNS_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN6936039.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(51224..52183)
                     /locus_tag="ACHGNS_000053"
     CDS             complement(51224..52183)
                     /locus_tag="ACHGNS_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFN6936040.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSKKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(52195..53706)
                     /locus_tag="ACHGNS_000054"
     CDS             complement(52195..53706)
                     /locus_tag="ACHGNS_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFN6936041.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(53728..54291)
                     /locus_tag="ACHGNS_000055"
     CDS             complement(53728..54291)
                     /locus_tag="ACHGNS_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFN6936042.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(54297..55160)
                     /locus_tag="ACHGNS_000056"
     CDS             complement(54297..55160)
                     /locus_tag="ACHGNS_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6936043.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(55174..56070)
                     /locus_tag="ACHGNS_000057"
     CDS             complement(55174..56070)
                     /locus_tag="ACHGNS_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6936044.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(56245..57534)
                     /locus_tag="ACHGNS_000058"
     CDS             complement(56245..57534)
                     /locus_tag="ACHGNS_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN6936045.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(57506..59023)
                     /locus_tag="ACHGNS_000059"
     CDS             complement(57506..59023)
                     /locus_tag="ACHGNS_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFN6936046.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            59221..60111
                     /locus_tag="ACHGNS_000060"
     CDS             59221..60111
                     /locus_tag="ACHGNS_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFN6936047.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(60184..61008)
                     /gene="nadE"
                     /locus_tag="ACHGNS_000061"
     CDS             complement(60184..61008)
                     /gene="nadE"
                     /locus_tag="ACHGNS_000061"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFN6936048.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(61020..62492)
                     /locus_tag="ACHGNS_000062"
     CDS             complement(61020..62492)
                     /locus_tag="ACHGNS_000062"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFN6936049.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(63129..63491)
                     /locus_tag="ACHGNS_000063"
     CDS             complement(63129..63491)
                     /locus_tag="ACHGNS_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFN6936050.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            63714..64466
                     /locus_tag="ACHGNS_000064"
     CDS             63714..64466
                     /locus_tag="ACHGNS_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFN6936051.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(64606..65529)
                     /locus_tag="ACHGNS_000065"
     CDS             complement(64606..65529)
                     /locus_tag="ACHGNS_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN6936052.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(65544..65816)
                     /locus_tag="ACHGNS_000066"
     CDS             complement(65544..65816)
                     /locus_tag="ACHGNS_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936053.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
     gene            complement(66160..67347)
                     /locus_tag="ACHGNS_000067"
     CDS             complement(66160..67347)
                     /locus_tag="ACHGNS_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFN6936054.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            complement(67454..67666)
                     /locus_tag="ACHGNS_000068"
     CDS             complement(67454..67666)
                     /locus_tag="ACHGNS_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein"
                     /protein_id="HFN6936055.1"
                     /translation="MLLLIGLILLIIGITKLSKANTIKKEAYELEQKNDEKLNKLHDR
                     ERFILSKEETLSDLNKQITMLKNDIN"
     gene            complement(67757..68209)
                     /locus_tag="ACHGNS_000069"
     CDS             complement(67757..68209)
                     /locus_tag="ACHGNS_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFN6936056.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(68222..68632)
                     /locus_tag="ACHGNS_000070"
     CDS             complement(68222..68632)
                     /locus_tag="ACHGNS_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN6936057.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            68907..69119
                     /locus_tag="ACHGNS_000071"
     CDS             68907..69119
                     /locus_tag="ACHGNS_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFN6936058.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            69122..69334
                     /locus_tag="ACHGNS_000072"
     CDS             69122..69334
                     /locus_tag="ACHGNS_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936059.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            69474..69629
                     /locus_tag="ACHGNS_000073"
     CDS             69474..69629
                     /locus_tag="ACHGNS_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936060.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(69626..69850)
                     /locus_tag="ACHGNS_000074"
     CDS             complement(69626..69850)
                     /locus_tag="ACHGNS_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFN6936061.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            69928..70116
                     /locus_tag="ACHGNS_000075"
     CDS             69928..70116
                     /locus_tag="ACHGNS_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936062.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            70255..70383
                     /locus_tag="ACHGNS_000076"
     CDS             70255..70383
                     /locus_tag="ACHGNS_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936063.1"
                     /translation="MAREEALKIGKVIADNRWGNSRPIILSKQHIEKQKAWQQIKK"
     gene            70442..70777
                     /locus_tag="ACHGNS_000077"
     CDS             70442..70777
                     /locus_tag="ACHGNS_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936064.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            71010..71951
                     /locus_tag="ACHGNS_000078"
     CDS             71010..71951
                     /locus_tag="ACHGNS_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda-exonuclease family protein"
                     /protein_id="HFN6936065.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            71953..72843
                     /locus_tag="ACHGNS_000079"
     CDS             71953..72843
                     /locus_tag="ACHGNS_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFN6936066.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            72886..73686
                     /locus_tag="ACHGNS_000080"
     CDS             72886..73686
                     /locus_tag="ACHGNS_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFN6936067.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            73701..74552
                     /locus_tag="ACHGNS_000081"
     CDS             73701..74552
                     /locus_tag="ACHGNS_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN6936068.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            74753..75214
                     /locus_tag="ACHGNS_000082"
     CDS             74753..75214
                     /locus_tag="ACHGNS_000082"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN6936069.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            75240..75692
                     /locus_tag="ACHGNS_000083"
     CDS             75240..75692
                     /locus_tag="ACHGNS_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936070.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            75798..75992
                     /locus_tag="ACHGNS_000084"
     CDS             75798..75992
                     /locus_tag="ACHGNS_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936071.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNGFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            76023..76409
                     /locus_tag="ACHGNS_000085"
     CDS             76023..76409
                     /locus_tag="ACHGNS_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936072.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQAKMTQISHLRLEL
                     EDLRQIEKSIQLNDNQKIVLEWLKSETILTREAPILSVNAFSDKNLLGKLPDKVRKAY
                     KLLACKQEYEVLSAFAQWGLEQEEAE"
     gene            76406..76609
                     /locus_tag="ACHGNS_000086"
     CDS             76406..76609
                     /locus_tag="ACHGNS_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFN6936073.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASNAIRKFGISILNDDRKWEEMR"
     gene            76606..76902
                     /locus_tag="ACHGNS_000087"
     CDS             76606..76902
                     /locus_tag="ACHGNS_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936074.1"
                     /translation="MKIKDGFYASSHGIGGLMLDMPIKNPKTRQKSKFKIGDMVRCKA
                     EEFIYPFRGYVEHVYNHSAIIHIENTMECDKWLAKSKDNLAVARLVDMELINDK"
     gene            76978..77394
                     /locus_tag="ACHGNS_000088"
     CDS             76978..77394
                     /locus_tag="ACHGNS_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFN6936075.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            77655..77792
                     /locus_tag="ACHGNS_000089"
     CDS             77655..77792
                     /locus_tag="ACHGNS_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936076.1"
                     /translation="MDKEIKVTVKLDLTELKELLNKASDQVEQLQETLDEIANFKIQV
                     S"
     gene            78202..78432
                     /locus_tag="ACHGNS_000090"
     CDS             78202..78432
                     /locus_tag="ACHGNS_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936077.1"
                     /translation="MRNYWYISLTNEYPRTIDDCSVRVVRSVQIKKKYSIVEMTREAT
                     PKEIDKCKLLYCGHGFYDEPNIQNNINKNLRD"
     gene            78591..79424
                     /gene="terS"
                     /locus_tag="ACHGNS_000091"
     CDS             78591..79424
                     /gene="terS"
                     /locus_tag="ACHGNS_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFN6936078.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELKSNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPKRNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            79417..80832
                     /locus_tag="ACHGNS_000092"
     CDS             79417..80832
                     /locus_tag="ACHGNS_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HFN6936079.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPENMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTTVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            80844..82373
                     /locus_tag="ACHGNS_000093"
     CDS             80844..82373
                     /locus_tag="ACHGNS_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFN6936080.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEYMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKDPTAKAEYVTKKLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            82291..83343
                     /locus_tag="ACHGNS_000094"
     CDS             82291..83343
                     /locus_tag="ACHGNS_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFN6936081.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            83345..83662
                     /locus_tag="ACHGNS_000095"
     CDS             83345..83662
                     /locus_tag="ACHGNS_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936082.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            83789..84412
                     /locus_tag="ACHGNS_000096"
     CDS             83789..84412
                     /locus_tag="ACHGNS_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFN6936083.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            84427..85317
                     /locus_tag="ACHGNS_000097"
     CDS             84427..85317
                     /locus_tag="ACHGNS_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HFN6936084.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            85340..85555
                     /locus_tag="ACHGNS_000098"
     CDS             85340..85555
                     /locus_tag="ACHGNS_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936085.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            85567..85908
                     /locus_tag="ACHGNS_000099"
     CDS             85567..85908
                     /locus_tag="ACHGNS_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936086.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            85908..86279
                     /locus_tag="ACHGNS_000100"
     CDS             85908..86279
                     /locus_tag="ACHGNS_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936087.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            86272..86667
                     /locus_tag="ACHGNS_000101"
     CDS             86272..86667
                     /locus_tag="ACHGNS_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFN6936088.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            86669..87046
                     /locus_tag="ACHGNS_000102"
     CDS             86669..87046
                     /locus_tag="ACHGNS_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFN6936089.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            87047..87655
                     /locus_tag="ACHGNS_000103"
     CDS             87047..87655
                     /locus_tag="ACHGNS_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFN6936090.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            87655..87996
                     /locus_tag="ACHGNS_000104"
     CDS             87655..87996
                     /locus_tag="ACHGNS_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936091.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGPVVEEAAEVMEG
                     MQNAVDGKSVSEALSRAYSFIADILFEGQFTGEDYRQGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            88238..91027
                     /locus_tag="ACHGNS_000105"
                     /pseudo
     CDS             88238..91027
                     /locus_tag="ACHGNS_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
     gene            91017..91757
                     /locus_tag="ACHGNS_000106"
     CDS             91017..91757
                     /locus_tag="ACHGNS_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFN6936092.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            91754..>93591
                     /locus_tag="ACHGNS_000107"
     CDS             91754..>93591
                     /locus_tag="ACHGNS_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFN6936093.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT        29001 a        17555 c        17042 g        29993 t
ORIGIN      
        1 atcggtgccg aagccattcc tcgacctaat tctttttcaa attttcctat tgttgatatc
       61 tccatataaa acggtgatac tggtttagac aattctcccc tgattgctaa cggacgtaaa
      121 ctacgttcca aattatttat gactttagga taatactgac ctttttctaa gcctgtcttt
      181 gcgtctatca aaagtatacg ttccttttct ttgatctctg gatttaaaac gagatcatat
      241 aattttagta ccatgttttc tatttcttca cttttagaca tcacgatttc ctcccaaaaa
      301 tatatttctt cccactacat tctataaaca aagtatacgc tacatctaga tttttttaaa
      361 tcattactgg attgttaaaa cctttcaata cactgttatg gcaacgattt ttattttaaa
      421 aacattctaa attaaatcag taaaaatttt tattctaaga tttttattag aattacgcct
      481 aatttgtgtc tctcagggca attctaagaa cgtttcaaga gatagcgaca ctatcgttat
      541 cgaaaaaggc gaaaacctat ctcattttct gctaatgtaa gttggtttag cttttgttaa
      601 tatgacttta tgaaaatgga ggtaattaaa tatgattttt cctaaagtta atgaagcaag
      661 cttgaataaa ggatcctttt ttcatatcta tgaaaacgaa gattttgaac tgtataatat
      721 cctttcacaa aaagaggtca tcgaaaacca aataatctgc catccaatta tttacaaatc
      781 tattttcaag tatgcatttt ttgcttccaa tgattttcaa agaatggaga ttaccgactg
      841 tatattcgaa aactgtgatt tttcaggctg tatattgaca aaaagcatgt tgcatcgcgt
      901 gaaatttgtc aattgtaaat taacaggtac taaattgatg gaaagttata ttgggaacac
      961 gttattcgag aattgtaaaa tggactatgt aaatttatct ggttcaaaca taaaagaaag
     1021 tacctttgag cactctgtct tatcaagcgc agattttgta gactgttccc tcactaaaat
     1081 aatgttttct actaatgatt tagaaaatgc tagctttttt gatactgatt taaaaaatat
     1141 cgatttaagt agcaactcct ttgaaatgat tgaagtacgc tcggaacatc taaaaaattg
     1201 tacgcttaat cagttgcaag cacttggttt tgctagaaaa tttttacaaa taaaagtagt
     1261 ataatcaaaa tgaatatata agataaattt ttagcaatac aaatatccat aaaaaaacat
     1321 tgcctatttt aactcttcat gtatactgac tctatcttca aaaactgaat agtaggagtt
     1381 attatcatga ataacatttt aggaaaaaaa atagaactta aaaaagggat attgggcact
     1441 attgtcgttg tactcggttt gttagggttg ctatttgcgc catccattat tagcattttt
     1501 tctttaacta tcactggatt tatagcattt acacttgttt ttggtaaaaa agatacgaaa
     1561 aaaatgttct cgaaacctgt cgctcctgtc aaaaatatag ccaaatattt ttttatcaat
     1621 gtagtaatca gtgccgcagt ctcggtattt ttgcaacaaa ttttgaaatg gggtttaaca
     1681 ggcaatccaa taaatgaggc tttcagcccg ttattattta ttattttgcc tatcatgata
     1741 cttggagaag aattgttctc agtgtatttt ctcgctattt tttctagtaa gttcagcatt
     1801 cctgtcgcta gtttcttgtc agcaatcatc tttggattta tccattacag tacttatgat
     1861 aatggaaata ttttacacac tgtagcacac atccttttga tacaaggggt ggcaagattg
     1921 ttattcaacc aagctgccat taaaagcaac tcgatcatta caagttgggc agttcatgtg
     1981 atatttgatt tcacagctat attgctagtc gttttattct cctaaatttt tatagaaata
     2041 atacatgctc tattgctaag aaagcatact ttatttcctt taaacaaaaa gacagatgta
     2101 tcaatggatt aaaaatcctt gatatatctg tcttctcttt taatgccagc tatagggatc
     2161 gaacctacga cctacgcgtt acgagtgcgt tgctctacca actgagctaa gctggcctta
     2221 tccaaaagaa attatatact ctatttggct gattgtaaag agtccgatgg aaaattagtt
     2281 taatttcatg atatttttca taaaacgtgg tataattgat gtgaaaggca ggttttatga
     2341 aaatggcaac ttttggaaaa tttgatacaa ctatcgtacc atcggaaatt ggtgaaaaat
     2401 tcaagatcaa tactcacgta atgttcacaa ttcacggcaa gtcgcaaact ggaacgattt
     2461 caaagcaatt gaaaaattcc gctgttgttg agattgatga aactgcagaa aatgcgaact
     2521 tgatgacgca aagtaagggt gtagtaatcg ttaactacaa agaactaaaa aagatttgaa
     2581 tttgaccatc tctgcatttt tgggatggtt tttttatatg caaacgaaag ctgtgaaaaa
     2641 gctgttctgc atcaaacgtt aaaaatgacc aaaaaaggat gtgacaaaag ttgtgtcaca
     2701 ccctttttcg tatttactta ttttgtacca aacaaacggt cacccgcatc ccctagtcca
     2761 ggaacgatat aaccatgttc attcaatttt tcatctagag aagctgtata gatgtcaata
     2821 tcaggatgcg cttcttgcaa agcttttact ccctcaggtg ctgctaccaa acaaacaaat
     2881 ttaatgttcg acgcgccacg ttctttcaat gcatcgattg ccatgattgc tgatccgcct
     2941 gttgccaaca ttggatctac tacaaacaac tgacgtgcat caatgtcttc tggaagtttg
     3001 acgaaatatt catgtggttc caatgtttct tcatctcgat aaagaccgat atgtcccact
     3061 ttagcagctg gaatcaattc caagataccg tcaaccattc caatacctgc gcgcaagatt
     3121 ggaataatcg ctactttttt accagacaac gttttttgtg ttgtttcagt gatcggtgtt
     3181 tcgatcacga tgtcttccaa tggcatatcc cgtgatactt catatgccat caacatagcg
     3241 atttcatcga ctacttcacg aaaaaccttt gttccgcagt ttttgtcccg aataatagtt
     3301 agtttgtgtt gaatcaatgg atgatcgatc acttgaaatt tgcccatttt ttaagctctc
     3361 ctttgttctg tcgtttcttt ttaattgtaa aacaaaatca agtgattgac tagtcaaact
     3421 cgccattttt cctaagaaaa tccgtttttg aaaagaagtt ttcctgcgga caagcaaagc
     3481 ttattctttt ttcttgatcc aagaagtaga taatgccatt gctgcacgcg ttgcctttgt
     3541 ctgatccata gcatttacca tgacaaaatc atggatgatt ccttggaagc gtacttgtgt
     3601 aacaggaacc cctgcatcac gaagtttgcg cgcataagcc tcaccttcat ctcttaatac
     3661 atccgcttct cccgttaaaa tcattgctga aggaagatca tttagttcat ttatatttgc
     3721 tctcaaaggc gaagctgtga tttcagcacg ctcttcttca ttcgtcgtat attggtccca
     3781 aaaccacatc attccttctc ttgtcaaaaa atagttttct ccgaattcat gataggaatc
     3841 tgtatcaaaa tcagcgtttg tgactggata atacagcaat tgcttggata ttgggaggcc
     3901 ctttcgttct tttgttaaga tcgtcatgac tgtagccatg ttaccaccta ctgaatctcc
     3961 tgcaacgatt agattctcga gatccagatt ttttgacttg gctaatttat tcagctgttg
     4021 caacacagca taattttgtt caattgctgt tggatatttt gcttctggtg atcgactata
     4081 ttctggaaag acaacgacgg aattcgtacg aaccgcaagt tctctgatca atttatcatg
     4141 ggtttgagca ctgccaaaaa cccagcctgc tccatgaata taaaaaataa ctggtaattt
     4201 atccatattc ccttctggtc gtacgagacg aatattgagt tttccccatt cgccagtatc
     4261 caccatcaag tcttcaatat cgacggaata tttgttcaca ggtgattctt gtacttcttc
     4321 tagtaaaact cttccttctt ccggtgctag ttcatagata cgcggatgag gcgcatttgc
     4381 atcactgaat gctactgctt ccttttctaa cgagatacga tcagtcatga cttttcctcc
     4441 ctgagattta gtgtgaagac taaaattaca cctcatatac tagagagcat caccgatatt
     4501 gtcaaagaat ccgttttccc aaaaaataaa cagaaaagcc caacattgaa cttttctgtt
     4561 tactaatgga atttaaattt ttatttttta tgcaacggaa atttttcagt taattcgcgt
     4621 acacctgttt tgacttcgtc tagttgagcg ttatcatctt ttgcttgtaa tgctttgaca
     4681 actagttcag ccacttttac agcatcttct tctttgaacc cgcgagtagt gattgctggc
     4741 gtgccgattc gaataccgct tgttttgaat ggacttagtg tttcaaaagg aatcgagttt
     4801 ttatttacag tgatattcac actgtccaaa atgctctctg cttcttttcc atttatacct
     4861 aactcgcgaa catcgatcaa catcagatga ttatctgtcg cccctgaaat gactctggtt
     4921 ccaattgctt gattgaaaac tttgaccatt gcttttgcat tggcgatgat ttgttcacta
     4981 tattctttaa atgcaggatc taatgcttct ttgaatgcta ctgctttacc cgcaatcaca
     5041 tgctctagag ggccgccttg aataccagga aataccgcac tatttatttt ttttgctaat
     5101 gcttcatcgt ttgtcaaaat catgccgcct cgagggccac gcaatgtttt atgagtggta
     5161 gtcgttgtga tatctgcata aggaactgga ttaggatgaa gtcctgctgc tactaatcct
     5221 gcgatatgtg ccatatctac catcagtttt gcgcctactt catctgcgat ttctctgaat
     5281 ttcgcaaagt cgatcgttcg tccgtaagca ctggcacctg ctacgatcaa ttttggttga
     5341 tgttttctag ctaaaatacg taccacatta taatcaataa cttctgttgt cggatcaaca
     5401 ccgtaagcaa caaagtgata ggttttgcca ctgaagttga ctggtgatcc atgtgttaga
     5461 tgtccgcctg cggaaagatc cattcctaag atcgtgtccc ctggttctac caaagcgaga
     5521 taagcagccg tgttggcttg agagccggag tgaggctgga cgttcgcaaa tttcgctccg
     5581 aataattctt ttgcacggtc aatcgctaaa ttttcaacga tatcgacaaa ttcacaccca
     5641 ccataatagc gatgccctgg ataaccttct gcgtatttat ttgtcaagat acttccttgt
     5701 gcagccatca ctgcttcgga gacaaagttt tcagaggcaa tcagctccaa attatgttct
     5761 tgtctttctt cttctttggc gatcgcagcc caaagatcag ggtcaaacgt tttgtaatct
     5821 accacaaaca acagctcctt tttattttcc tacaatttta ttttcctaca attttatttt
     5881 tcccatcaaa aacaaattgc caaatattcg tatctcttct attacgatta gttacgctta
     5941 gtttttcgtt cactgataga agatttactt gatagcattg tatcatacta aaagaatgcg
     6001 acaataacca attgatcatt tttcaaaata attttgtcct gctgcttttt tcaagcgatt
     6061 catgtaagca ttccccaatc cagtttctgg atacacttgt acaaaaatca aatctaatcc
     6121 aagtgttggt tcatctaatc ctcttagacc tgcaaacaat cctttagccg ccgcttctac
     6181 tgagttgtcg ttatacatat aaacagctgc agtatctgtc cgcacttgat ccgcaatttc
     6241 ggggcttgct atgacacctg cacggatctt cttatttttt gcccattgaa ctgcagttga
     6301 ccaatcgcca tctctgacca tcagaacacg tgtatcgggt gagtagtgtt tgtatttcat
     6361 cccaggggct tttggtgttt cattttcttt tacaagatgc tgatcaatag caacaggaga
     6421 ttcgatgact gcttcgattt gttctttcgt tactgcacct ggtcgtaaaa tcataggcat
     6481 ggctgttgga tcacttaaat caagaacagt ggattctaca ccaattcgcg tagctccatc
     6541 atcaataatc ccggtaattt ttccttgtag atcatgatat acatgatctg cagtcgttgg
     6601 acttggtttt cccgacgtat ttgcacttgg accgacaagc ggaacaccgg aaagttcgat
     6661 tacttctagt gtttttttgt tatccggtat acgaaatgca gctgtggata atcctcccgt
     6721 cacaactgag ggcaaagtgt cttttttgat cttaaatatc aaagttaatg gtccaggcca
     6781 gaagttcttc acaatcttct ctgctgccgg atgaaagttg tccacatact cttttacttg
     6841 ttcaaaagag gcgacatgga cgattaatgg attatcttgc ggtctccctt ttacagaaaa
     6901 cactttcttg acagcgtttg gtaaaagagc gttggcgccc aatccgtata ctgtttcggt
     6961 aggaaaagca acaagttctc cttctttcag tgcttcggct gcctcttgca attctgtttc
     7021 atcatatcgt ttcgtttcca cagtttatcc acaacccctt tttcatacat tcttattttg
     7081 tctgagttta tcctcaagtt ttccactgtt tgtggataat ctatttgtca atagtggata
     7141 aacttgttga taaaccaaaa agtcgtttac atccttgtca aataagtgtt tttttataat
     7201 aaaaaaacaa ataaaaaatt tttcatccac aacttcaacc ataaacttgg atcattcgtt
     7261 cattgccaaa aagatccttt tttatcgcca cttttttatc tggaaatgct tgctgaaata
     7321 ttttttttac tgcttcacct tggcgaaatc cgatttctaa aaaaatcttt ccatttgatt
     7381 ttagtacgct tggtgcttca actgctattt tttcataaat agctaatcca tcgttctcag
     7441 caaaaagggc cattttaggt tcgaatgacc ggacactttc atccatcagt tcccattcat
     7501 tccgactgat gtacggcgga ttagaaataa tcacatcaaa ttgctgatcc attactggtc
     7561 ctaatgtatc tccatgataa aaactgattt tcgttccttc tttctgagca ttttctttcg
     7621 ctacttctaa tgcttcttct gaaagatcca cagcagatat atgccaattt tttcttgctg
     7681 ctttcaaact aatagcaatt gctccagtcc ctgtcccaat atctatcact tctaacggta
     7741 caccaggcgt ctcgtctaaa caccattcga ctaactcctc tgtttctggt cgagggatca
     7801 gtgttgcttc agttaccttt aaccgatgat cataaaaatc tgcatatcct agcaaatact
     7861 gaggtggatg atctgcaagc agaagtcgca gatcggtttc gatcaaccgc tgttcttctt
     7921 ctgaaatctc ttcgttcata tgaaggagcc aatcaagttt tttccattgt tttcgttcca
     7981 aaaataaaaa ttggatacta tatgcttctt tcccatgatc ctctaaaaaa gaagaagccc
     8041 gtgacaggac ttctcgatag gtcattttac ccattttgca tagcttccag ttttgatgtt
     8101 tgatcataca tgatcaatgc atcaacgatc tcatctaatt tccctgctaa aatctgatct
     8161 aatttttgga tagttaatcc gatacgatga tcagtcacac gattttgcgg gaaattatac
     8221 gtccggatcc gttctgaacg atccccagtt ccgactgctg attttcgatt ggcatcatac
     8281 tcgctttgtg cttcttgctg gatcttatcg tacacacgag cacgcaagat tttcattgct
     8341 ttttcacggt tcttgatttg agaacgttca tcttgcatag caacaacgat ccctgttggt
     8401 aagtgagtca aacgtacagc agatgctgtt ttattgacgt gctgtccgcc agcacctgaa
     8461 gcatgataga tatctgtacg gatatctttg tcttcaatat cgatctctac ttcttcagct
     8521 tctggcatca cgacaactgt tgcggtggaa gtatggatac gcccttgcga ttcagttgaa
     8581 ggaacacgct ggacgcggtg cgcaccactt tcatatttta atttcgaata tacgttgtct
     8641 ccggtgatca tcatgatcac ttctttatat ccaccgattc cggtaacgtt agcttccatc
     8701 acttctgttt tccagccttg tgcttcagca tacttttggt acatattgaa aagatcgcca
     8761 gcgaataatg ctgcttcgtc tccgcctgct gctccacgga tttccatgat gatattttta
     8821 tcgtcatttg gatcttttgg taaaagcaag atcttgattt tttcttctaa gacttctttt
     8881 tcttttttta attcagataa ttcttctttt gctagttcag ccatttcatc atccagtttt
     8941 tcaccaagca gttcttctgt atcggtgatt ccttgtacta cttctttata acgacgataa
     9001 acttcgactg tttcgcgcgt gtttgcttct tcttttgaaa gctgcataaa acgctttgta
     9061 tcagaaatca cttctgggtc acttaaaagt tctccaagct cttcataacg gtcttcaatt
     9121 gcttgtaatt gatcgtacat gcttattctc cttctgtaat cattgatggg tgaaagtaat
     9181 gttttcgaca aacggggtaa taagcttcat ttccgccaat ttgcacttga tcgccttcat
     9241 aaacagggtt tccatcaata taatgtaaat tcatgattgc ttttttgtgg cagaaccaac
     9301 agattgtttt catttcttcg attttgtctg cataaagcaa taaatatttt gatccttcaa
     9361 ataactcgtt tcgaaaatca tttttcaaac caaatgccat aaccggaata tccaattcgt
     9421 ccacaattct cgtaaaagca atgacatgtt tcttttttaa gaattgcgct tcatcgatca
     9481 gcacacaata gggtttgctt tccattttct gaattacgtc aaatacgtct gtttcttcaa
     9541 atatcggcat cgcattacgt ttcagtccga ttcggcttga aacaacgcct aaaccatctc
     9601 ttgtatcaat accgcttgtc attaagacaa ctggcttatt ttgttcttca taattatgtg
     9661 cgacttttag gatctcgatc gtcttgccgc tattcattgc gccatactta aaaaatagtt
     9721 gtgccatgct ctttttcacc ttccagtttg ttcctatggt attatacttg attttcagca
     9781 ttttttacag tgagaatttt catcaagcac agattagtat taaatctatt gctcactttt
     9841 gttccaattt tttgacaaga actaaacatg ggttccattc atcccataaa tttggaaaaa
     9901 cttccaacct tgaaaatcct aatgattcgt agaaacaaat agtttgatca tatatagagt
     9961 agtgcccctc gtctacagtc ttcacttgca aatatttgta atgttttgct gcgtaacctt
    10021 ctgcttcttt aaaaagtttt gttcctaatc cttggtgatg atagtctttt tttattccca
    10081 tacaatagaa tagatttcgg cagtatcttc acttgtctgt gaaagagtga taaagcctaa
    10141 atattcttct tttctttttt cacaccataa aggtagttta cttgattcct cgatatattt
    10201 ttccgttgat tcaggaagcc caaaccactc aggcaagtct agtaatattt ctcggacaca
    10261 ctttcctttt tcctctctat cttttacaaa cgatatcggc atattctttc tagattctcc
    10321 ctttttgaat tgagtatagc atgctttctt tcttttcacg tgctataata agttaaaatt
    10381 tcaaagtttt acgtaaggag ctatttgatg acaaaaaata tttttgattc catcgctaac
    10441 cattatgatt cccctgaccg tcaagcatta gccaagatta ttcgcgaaga actaactact
    10501 tatctaccgc gcgacagcca ccaaaaagtc ctgctggatt acggaggcgg aactgggctt
    10561 gttagtttac cgcttgcgga aagattcaaa gaactgatca ttgccgatgc ttcagaaact
    10621 atgctgaaaa tggcagaaga aaagatccaa gcagcagatc tgaaaaatgt ccgaacgatc
    10681 cacgctgatg cttctgtcga atttcctgct gtccaagcta atctcatcct tctatcactt
    10741 gtacttttac acattcctga tacggagaat attcttacaa aactttatga aattttagct
    10801 cctggcggtc aattgatcat cgtagacttt gacaagaatg aacaaattag tcatccgaaa
    10861 gtccataatg gctttagcca agaagaattg aataatcgat tggaaaaaac gggctttgtc
    10921 tctgctacta gtcatacctt ccatcgtggg gaaaagctat ttatgaacaa aaacgcttcg
    10981 cttttcttat ccatcagcca aaaagcttaa aatgagattg caaaaaaagt gagcaactcc
    11041 aagaaataag aaggaatttt cggaaaaaga agctgtgaca aaaaacatgt cacagcttct
    11101 ttttattttt tcatcacatg attttaattt ctgaaagagg ccaataacgg aaaacaactt
    11161 ttccttggat catatctttg tcgactaacc caaacattcg gctgtctttt gaaatcaagc
    11221 ggttatcgcc tagaacaaga tactttcctt ttggcacagt atattcaaag tcactcgtaa
    11281 aggtggatga actttctgct tgtgcttgga atcctgagct ataagcatat tctccttgta
    11341 acttatcttc tgaaaattct tttttgaact catccagata aggttcatca tactttttgc
    11401 cattaatggt caactggtca ttttccatct tgactgtatc tccaggcata ccgatgatcc
    11461 gtttaacaat catccgttct tcatcccccg gttcctttgt agtaataata tccattcgtt
    11521 catagtttga gatcttagat gaaatcaatc gttgtccatc acttaatgta gggtccatcg
    11581 aatgcccgtt tacagtaact ggtgtaaaca cgtacactct agctaataaa atagccgcta
    11641 atacaaggat caaaatcccc caatttttca aaaattcttt cacagcttac ctcctacttg
    11701 tattttcctc gttatttatt acgttttttc acaatacttc gttgtacttt ctttattcta
    11761 caatgtttat aaatgaaagg aaagaataag tgatgtgttc gtcttatctt tctcgttata
    11821 taaaaaagga acgtgacaaa agacgtgtca catccccctt attccgaata aacgttttta
    11881 tcgcaaccac tgatacacgg cgtttcatca cattatgaat gtcttcttct ttggctacga
    11941 ctataaacca ctggttcttc ctgcgactgt gccttttttg caatcaattt cagacgataa
    12001 ggctcatgat agcgtaaccc gtctttgaca aaatcaccgc cgacgatgaa caatccgaca
    12061 ggttccaatg tgtcttctgt tgagtcaagt cgaatggtcc ctactttttc catgtccgag
    12121 tagcttcgtc caatatttag aacatactct ttctctcctg ttttcttgac atagtaataa
    12181 ggatacaatg tatttctatc gaccagaaat ccatccatgc gaccgagttc atttgttttg
    12241 atctcaccat ttccaatcaa ataccgcata ttctcggcta atttcaattg ataatcttcg
    12301 tcataagaaa ccatgacctc ttgtgtattc actttatcga tcgaatttcc attatctatc
    12361 gattcttctg tctggatgtt gaacgcttca tcaggtaaat acttcacaaa aagctgagtg
    12421 acatcggctt tcgtgttttt atctaaaaag taataaaata catttaaaac agtaaaataa
    12481 acaagcacta gaaatgcaag tccatataag aaaaattgaa gatagttttg gttttgccaa
    12541 agtccataaa caattctcaa taaataaagc gtgggaatca cacttaaaat cgtatatcca
    12601 cggttaagat atttaggact gatgtctaaa tagccaagga atttatgcac cccgttgata
    12661 aggtctagta aaaatgtcat tgagaatcac ctgcttgctg gttatacgtt tgggtatttc
    12721 ctgaatcttg gttttgctgt gctgtacgtg ctgcatcatc attttgcgtg ttatcatcta
    12781 cagtcgtact gcttggtatc gtttcggagc tagcacttgt atccggactt gtacttggat
    12841 tggttgaatc cgatggagtg ccatctggta ccgtattact tgtttcgctt gagctgtcag
    12901 ttgtatatgt ctcatcatac gacgaactag ttgttgtatc gtcccatgta cttgtcgtag
    12961 tggtttcatt caaactttcc gttgttgttg gttcagatgt cgagactgaa gtagttggtg
    13021 ttgtttcttc tacagtacta gaacttgaac ttggtgcacg gtagctggaa ctattcacaa
    13081 tacctgtagt agtcgcgaca atcgtcgaga ttgttaatac agcgattggc ttcaaaatag
    13141 gtgctacttt tcgcatctta tctcttcctt cattgaaatt tattcttgtt tccttacttt
    13201 actacggaca aattatacag tctaccttat ttctaccata aattctttat ttttttctta
    13261 cgtttttccg aaagatttct ttaattttcc tcaaaaacta cggttgatgt ttttaagtaa
    13321 gcttttgctt cccgattgat ttctttcaga tcgcatcctt gctgcttggc agcgaacacg
    13381 caaccacaat aacattggcg ataaatatca tactctttgc acatttgcaa agagcgttca
    13441 tagcctttgt ttttcttgaa atcacttggc aagtattgtg tcccgtaaat cttttggata
    13501 tccattccga tctgattgat caaagctgca tttttctttg gcgagatggt cagggcactt
    13561 ccaaagtaat cgtatcctag ttcttgcgct ttttgcgcaa caagatctaa tcgcatattg
    13621 aagcatgcac tgcagcgagc accaccttct ttttcgtttt caagatggtt tgttaaaacc
    13681 attttggtga attcatttgg acggtattca tcaacgatta gcgcaacatg atggtcagtt
    13741 ttctcattaa attggtctac aaatcgtttt tgttcatgta atcttcgcaa atactcactt
    13801 ttaggatgaa tattcgaatt tgaaaaaaat aacgtgatct ctgcatattg agacatgaat
    13861 tccaatgtgt gcgtactgca aggagcacag catgtatgta ttaatatctt aggctttact
    13921 tcttctttct cccattgcat gatcattttt ttcagaactg tatcataatt tattttttga
    13981 tttttcattt tttctacgat ttgttgagca ttaatcaatt tgttattcct cctccttctt
    14041 ttttctagga ttttccttat tataagccat ttctatttta atcgaaagct gtattgttat
    14101 ctgtttgata atttctttga gtattcctaa ttccccttgt atactggtag aaacgactat
    14161 tgtcaggagg atagtactgt gcatgttgat ttattctttc gaaggctgca gcggcgagga
    14221 cagaagtttg ctgccaatca tttctcatcg atccaaatca tcgtttttta ttatatattg
    14281 atgacggtct tatcactcgt tctattttat atgccgattt ttcgcgaacc agatagccat
    14341 gtttcttttg tggacatgct gttcatggca atcagtacag tgagtgtgac tggactatcg
    14401 acttttgata tcaattctgt gttcaatgat aacggtatta tactgctgga aatattattt
    14461 caagttggtg gtttggggat catgatgatt tctactgctt ttgtcatttt ttctaaacga
    14521 cggatcacac tgaaacagcg acagctgatc atgacggata tgaatcaacc gcgtttgtct
    14581 ggtatcgtcc gactgattcg gatcactttt gctattttaa tctggtttca attacttttt
    14641 gggacattct tttctattta tttttattac agaggctatt ttgatcgttg gcgtgatgcc
    14701 gttttttacg gtttttatca ggcgatatcg gctgtcacga actctgggtt tgacgtcact
    14761 ggggattcca tcaagccctt tgcacatgac tattttttct tgatcctgat catgtttttg
    14821 atttttgtcg gaggaattgg ttttcctgta ttgatggagt gccgggaatg gctattatac
    14881 aaacgttcca atgcgaagct tccgtttcgt ttttccttgt ttaccaaatt agctgtgtta
    14941 gcttttgtta tcttgtttgt ttcgggaact gtcttgattt atctattgga aaaggaccat
    15001 ttattccaag attccaatat gagtgtcaaa tggatcaatt cgatgtttta ttcgattacg
    15061 actcgtaatg ctggtctgca gattcacgat ttgggtgatt ttcaaatcac aacactgatc
    15121 attttttctt tactgatgtt tatcggatgc agtcctagtt ctgtaggcgg cgggattcga
    15181 acaactactg tagcaatcat cggtttgtat ctatattcct tcctcaaaag tgaagataac
    15241 atcaatattt ttggacggag aatcgatcaa gatgatgtgc gaaaatcagt cgttgtcttt
    15301 atgctttctc ttggtatgtg tttcttttgt atcgtttttt tatctgctac agaagaacaa
    15361 acgctgatct cgatcatcat cgaagtaact tctgcgtttg gaacaactgg tttatcttta
    15421 gggattaccg gagacttgtc tgttgtgggc aaaatcacga ttgctacact gatgtttatt
    15481 gggcggatcg gaatgctata tacattgatg ttattcgtgc caaaagaaac acgagatcta
    15541 ggctatgaat atccaagcga gaaaattatt atcggttaaa atgggaatat aggtaagagg
    15601 gaataataaa atcacagcag cttgaaacaa tcattttgtt tcaagctgtt tttttgttaa
    15661 atcatgcgat tttgtccgat ttcatgcact ttcagccgta accttttctt aagaataaga
    15721 aaagcaattt aacctttttt tcaacatatg gtatgattag ttgaatttat tcgtcagcaa
    15781 atcgtttttg gaggttacat aatgggaata cgcagtcatt ttgcgattgc agttggaaaa
    15841 acgtcgcaat gggcattaaa aacttttttt aaaggcggat caagtttacc aggtaaacta
    15901 gcgctgaaag tcgatccaca tatactagat agtttggcaa aagactacca agtcgtcgtt
    15961 gtcaccggaa caaacggtaa aacattgact accgctttaa cagtcaatat tttgcgccag
    16021 caattcgatg aagttctaac caaccctact ggtgcgaata tggtacaggg aattgtatcg
    16081 actttcttgc aggcaaaacc aaaaaaagga cagaaaaagt ttgctgtcct agaaatagat
    16141 gaagcaagtt tgagcaaagt cacagaatat atgaaaccag aactgttctt atttacaaat
    16201 attttccgtg atcaaatgga tcggtatgga gaaatctata cgacctataa aatgattgta
    16261 gacggtgccg caaagtcacc aaatgcaacg attatcagta acggtgattc accaatcttc
    16321 aattctatcg aaacagtcaa tcctagaaaa tattatggct ttgaccatga agaagatcgt
    16381 gaacaaatgg ctcattacaa tacagacggt gtcttatgtc caaattgtca tcacatcttg
    16441 cattataaaa tgatcactta tgcaaactta gggaaatatt actgtccaaa ttgtgacttc
    16501 aaacgtcctg aattggatta tcgattaact gatatggtac ggatggacaa tgtgtcagca
    16561 gatttcgtga tcgatggtca tgaatacgga atcgaagttg gcggtatgta taatgtctac
    16621 aatgctttag cagctactgc agtggcagag tacttcaatg tcgatcctga taaaatcaaa
    16681 caaggtttag gatatgacga gaaagtattc ggccgacaag aagtcatcaa tatcaatggg
    16741 aaaaaatgta cattagtact tgtcaaaaat cctgtaggta tcaatcaagt aatcgatatg
    16801 attggtctag ctccttatcc attttcttta gtggctttat taaatgccaa ttacgcagac
    16861 ggaatcgatg tcagctggat ttgggatggt aattttgaaa actttgccga tatggatatc
    16921 ccagctgtta tttctggagg agaccgtcat acagatatga ctttacgtct aaaagttgcc
    16981 gggattccag aaaataaatt gacagaaaca aaagaattac cagaagtaat cgaaaaaatc
    17041 aaagaattgc caactgagca tgtctatatt ttagctactt acactgctgt attgcagctt
    17101 cgtaaagaac tagctgctca aggatttatc aaaggaggaa tgaaccgtgg ctaattatga
    17161 actcaatatc gcccaccttt atggaaattt gatgaacaca tatggtgata acggaaacct
    17221 tttgatgttg aaatatatag cagaaaaaat gggggtttct tgccatacgg agatcgttag
    17281 tatacatgaa ccttttgatg ctgataaata tgacttggtt ttctttggcg gtggtcaaga
    17341 ctttgagcaa ttaatcattt caaaagacat ccagaaaaag aaagattctt tgatcaacta
    17401 tatcgaaaat gatggcgtca tgttagccat ttgtggtggc taccaattgc taggtcacta
    17461 ctacatggga gctaatggag aaaagatcca aggaatcagt gcactggatc actacacgtt
    17521 aagtcaggaa aataaccggt ttatcggtga tatcgttatc cataacgaag aattcaatga
    17581 aacttattat ggatttgaaa accacaatgg tcggactttc ttaggtgaag gtgaacgacc
    17641 tttggggaaa gtggtacaag gtcaaggaaa taatggtgaa gaccaatctg aaggtgtcat
    17701 ttaccgaaat gtcttcggtt cttatttcca tggacctatc cttgcaagaa atgagaacct
    17761 ggcagctcgt ttgatccgcc tagctttaga acaacgttac ggtaaagaat tggatctgcc
    17821 ctatcttgaa ggacaaacag cctagttatt cgaaaaaggg gaatgtgaca aaaatttcgt
    17881 cacattcccc tttttcgtat cctattttaa gcgacttgct tggcataacg gttcacgttt
    17941 tgcttgatat tcccgattgt tttcggatca ttgattcctg tgacttcttg aatcaattgt
    18001 tccaagtttt catggatctt catttcatgc aattgacggc tttgttcatc ttcaggatca
    18061 tagtaattaa aaataatccc cattgttgcc gtcaaatgtg ggcaagtcaa gttatgttct
    18121 tgtagttctc tcactggacg tgtaaatctt tcttgataac ctaacttacg taatggtgtc
    18181 cgagcgacac gtgtgatcgc atcagagata tttttgtttt ggaagcgatg aatgattttt
    18241 tcaatatacg catgatgctg ctctgcatca aacccccatt tggcaatcag caaacttcct
    18301 gtttcctgca aaactgattt taattgtgcc acaactaacg catcttgcat tgcttcatca
    18361 atggtctggt aacctaacaa ggcacctgta taagcgacag tcgcatgacc agtattgaca
    18421 ctaaatagtt tgcgttcgat atatggttct agatcatcta catataaaac cccttttaat
    18481 tggatttttt tattttttct ttggctatcg tcaatgaccc attctttgaa aggttccact
    18541 tggacgaata aaggatcttc atggtgttgt aatgggacga ttcgatcgac tgctgcattt
    18601 ggaaagccga tatatttgtc agtaaatgat gtttccggaa gatatttata cacttctttt
    18661 tctagaaatg ctgaaccgcc aatcatattt tcacaggcaa tgatatctaa agatgttgtg
    18721 cttccttcct tttctctttc ttggatacct tttgcaatca actcagcaat aaaaggtaag
    18781 atattcgggc caatggcggt agtaactaaa tctgcttcag cgatttcttg gattactttt
    18841 tctggatttg tttgattgtt gattcctttg acatttttta cagtgatttt ttcttttgat
    18901 tcatctgcta attcgatttc gtattctttt ctttcattta aagcatcaat gatcgtgtga
    18961 ttcacatcta cgaaagtaat ctcaaatcca ttagctgcca aaacctctcc aataaatcct
    19021 cgaccgatat ttcctgcacc aaaatgtact gctctcattt ttctctctcc ttaagcaatt
    19081 gctagatttt ccgttacttc ttctttgctg agcgcgtctg ctagctggac aacattgtcc
    19141 atatcactgc aataaagtgc gatttgctgt acaagttgca aatgctcatc tccgactcct
    19201 gcaataccga acaaaacagt cgcaatcttt tcttcttctt cggtaccaaa atctacacca
    19261 tctggtactt gaacgacaca gatgccggat tttttcacat atttttttgc ttcatctgtt
    19321 ccatgaggaa tcgcgataaa attccccata taaacagata acattttgtc tctatctacc
    19381 atggcatcga tatattcctc atcaacgcac cctgcttcta caagcatctg accgcaatag
    19441 cggatcgctt cttctttggt tttaaaagat cgattgagct cgatcatgtc gaatggcaaa
    19501 ctttgcatgc tgttttcctc ccttaagctg tctgtttcaa atattgtgca atcgattcgt
    19561 atttttgcgg attgaccatg ctgttgactg ataaatgaat ggattgaggt gcagccatct
    19621 gtactttttc cgctaaactt tctttggcta cgatcagtga atcaggttca tcttttaaat
    19681 cttggatatt gacttttctt aacgggatag ttaaatggtt cttgtctaat atatctttga
    19741 gcacggtcat ccccattgtt gctgatccgg aacgatcatc gtggacaagt agaatctctt
    19801 tgatttgttt gatgtttgct gaaagaaact cttctgctgg ctcattctca atttcagaaa
    19861 ctgatgtttt ctgcttgctg cttaattctt gaatgatttc atcatatcga ggtgaattca
    19921 aaaagttttc tactgagaca aaacgcgcgc tcggtgcttt ttgttttgct ctttcttgta
    19981 gttcgacctg agtaacgatc aatgtattcg agtcatctgt cagttggctg attgcttgat
    20041 tggtcaccgt ttgaggcaag tctgcttcct gtactttttt tctcaagatc gaagcgccca
    20101 tcgcacttga tcccattcct gcatcacaag caaaaatgat tttttgtacg ccagagaatg
    20161 tagctccttg aggaatacct tgggcacctt tggaagcttg cttagctgcc tttgtctctt
    20221 gaacagattt ttcgaagtct tcatttgtat ctttcgcatc cgctttcaaa attactgcag
    20281 aaataccaaa cgaaacagct gcggcaacaa tcacgcccaa aataacagga aggtaagaat
    20341 tgaacggtgt catcgctaga atcgcaatga tcgaccctgg agaagctggc gctcttagtc
    20401 cagcatccat caattggaaa atgaaacttc ctgacattcc tccgcctata actgataaga
    20461 ataataacgg tttcatcata acgtaaggaa aatagatttc atggatcccg cctaaaaagt
    20521 gaatgatcat ggctcctggt gctgaagatt ttgctgctcc tttgccaaac aaagtaaaag
    20581 caagtaatac gccaagtcct ggaccaggat ttgcttcaag caagaataga atagattttc
    20641 cagcctctgc tacttgttca gttcccaatg gtgtcaaaat cccatgattg attgcattat
    20701 tcaaaaataa gatttttgct ggttcaataa aaatacttgt caatggaatc aaatgggcag
    20761 ataaaatgat ctctacacct tttgccattg cttgagtcaa tgcatcgaca actggaccaa
    20821 tcgcagaaaa acctaataac gctaatccaa aaccgataag acctgctgaa aaattattga
    20881 ctagcatttc aaaaccggat ttgatccgtt tttggaatag ctgatcaaat ttcttgatgg
    20941 catatccacc aagaggtccc atgatcatcg cgcctagcat cattggaata tctgttccga
    21001 caatcacacc catcgttgca attgccccaa caactgatcc gcggtcacca gcaattactt
    21061 ttccgccagt ataaccgatc aataaaggaa tcagataagt tagcattggg cttaccatgg
    21121 tagcgaaagc ctcatttggt aaataaccat ctgggataaa tagagccgtt aatacacccc
    21181 aggcaatcag tgctccaata tttggcatga ccatgcttga taaagtactg cctaattttt
    21241 gtaccctcgc ttttagcgaa atgttgtttt tgggaatcgt ccgttccatt ttgtctctcc
    21301 ctctctcctt ttgatactct aattgtatcc gcttactaaa aagagaaata gtcgtacttt
    21361 ggcacgaaac tttgtgccaa aggtttgagt atgtcagcaa aataaaaaga tttcggtatc
    21421 agaaaacttc aattttaaat aaaactgtta ttttaatttt tttataatat gcaaacaata
    21481 acttttgtta gcatttaatt tagatgtttt ccattctatt atgttattaa attttatatt
    21541 ggaggcctat ctatgacatt actgatcaaa aacatgaaca tgaaacgtgg taaaaaacgt
    21601 gtgattcgac aattgaatct tttagtagaa tcaggagaag tagctgcttt gattgccccg
    21661 aacggatttg gcaaaacaac tttgctgaat ggcattgccc aactgctgcc aacaacttat
    21721 gatcaattgt cccttcacaa catctctcct actgcttata cagattatcg ccggtctttc
    21781 ttttttctag aatcaacgaa tcagttatcc gactatctat cggctgttga ttatttgact
    21841 ttgatcaaat actattggca gagcaaagta gaatgccaaa ctgttttcca acgattgaag
    21901 atgactgagt accttacttt gccgattgga aaaatgtctc taggaatgaa acaacatgtc
    21961 ttgcttgctg cttacttgat cagtgattca tctgtcatgt tattcgatga accgttgaac
    22021 ggattagacc caggaagtat tgatatcttg aatagcctca tcagacaatg gcagaaagag
    22081 gggaagacga ttcttctttc ttcccacgct atcgcgaata tccaatcgat ttgttctaaa
    22141 gcatgttttc taaaaaatgg agaaatcgtc acagaatcct caaatatgga agagatttta
    22201 gctatttatc gtaaattata tgaggtgtaa ctatgtcttt acttacattt gaacgcaatc
    22261 tcttactaaa aaacaaagta aactttttat ttcctctatt gttggtcgtg ctctttgcat
    22321 tcccactttt ttttgatcat aaacttgctt atacagaatt cgatgaactg aatcataact
    22381 atgaagagat gcaacgtttg attgaaacgt tgaaagaaga tgagaatgag aaagaatttg
    22441 tggaaagttt agagaaaagc aacaaactga tcgaagcaat cttacatgca aaaaacacag
    22501 ggaatgtcca gcaaatggtc gaagcaactt atcattatga aaaagatata ctggatcgac
    22561 tgatttctgg tcaaagacaa ggaattccaa tcattgaaca acaaaaacgt gtcgagttgc
    22621 tccgttatat gaaagaacat cagatccaaa gatattctat ttttgacttg ccagcgcatc
    22681 tctctttagc aaattactac gaaaatattt tttcaggaat gatttctagt tttttgattt
    22741 tatgtataac cgccttgttt ctttcttcca tcatctctta tgaaaaaaga aagcaggtaa
    22801 tctctctagt caatctatta ctggatagta tggtaaagaa acatagcatc cgttttacca
    22861 tttattatgg tgcagctatg ctaagtctcg tgatgccatt tttgatcgtc tctatactag
    22921 taataatcaa aaatggtttg ggagacttcc gttatcctgt aggaacgatc attggtcagg
    22981 agatacgaat cctgccgatg tatgagtatc ttttccagag ctttctgttc ctgttgttgt
    23041 gggtgctctt tttaagtaca atcagttttc ttctatctgc tttatttgaa cattctctag
    23101 tcaatctttt aggtacactt ctttgtcttt tcttagcaga gtatcgcttg ttttcttcga
    23161 ttggatggat agaatcaatc agtcattact tgccaacttc ttatgttgat tttcaaaatg
    23221 tgatcatagg aggagatatt ttctcgccac tagcttcaga acaggttact ttcatgaatg
    23281 gtatcttgac acttggtata tggagtatcg ttttattatt cataggtatg ggcactatct
    23341 atataaaaaa gagctattaa aacacaaaaa taataggggc ggtgacaaaa accttgtcac
    23401 cgtcccctat ttcaaataaa cggttttcta gaataaatag ttttcaaaag cttgcatccg
    23461 cagtaataag aacctcccaa caaaaatagc tcctcttatt tttatcttgg ttgaagttat
    23521 tacttgatgc aaggcagctt tttttactgt gaacattggt tatatatgtc catgttcaaa
    23581 aatcatttgg acttcttcaa tggtttccgc atcactgagc gccattactt ggtcgatgtc
    23641 tgaacagaaa aaggctaaat cttggagcac cgctaactga tgttcttttt ttaaagccac
    23701 tacaaataag agtgtcgcga tttttggttc ctcttttgtt ccaaagtcca caccatcagg
    23761 cacttggact aaatagattc cttcttctac tacttcttca tctttgtcag tcccatgtgg
    23821 caaggctacg aaattcccga tatagacact cgctttttct tgtctttcga tcattgattg
    23881 gatatatgcc ggtttgattc ccccttgttg ggtcatccac tggccgatct cggtcaacgc
    23941 ttctttccaa ttttgatagg attgatcgag caatattttt tctgctggta gctgtatcat
    24001 cttttcacct tcatttcttt atttcttcaa tcaggatttt cgctaacaac tgataaacga
    24061 tttctttatt tcctgagcgg aagatctcgg tgttcaagtc attcatgatg attgctccac
    24121 tgatctttcc taaaagaatc ccgtcttttt cttggattgg cgaaggcgcc agcataacta
    24181 acatacggga caaatcgact ggctgtctat ccatcccttc aatggtcaac ggatgtttca
    24241 aattgaaaat acaaaagatc ggtttagaaa tcaatgagct ggatgtatgg aataacccca
    24301 tatttgtatg aggaataccg ataggtgctt gttcatatcg cttaagcagc cggcgatgaa
    24361 ccacctcttc atccgttact agtccttcag gaaaataagg aaataagctg cgaatcgttt
    24421 cggaaatcgt ctcaggatta tccagttctt ggataaaaaa acgaccgagt aattgattga
    24481 tttgatccat gaaatgcatt gtttcctcat aagtatcttc tatcatccac tcgttattgc
    24541 tgttatacgt aattttttgc ctttttttct tctcaatatg cgaaaaagct tcttttagct
    24601 gcttgacttc atcttctagc agtagcgggg agactaaaat atattttcca ccgtatcctg
    24661 gtaaaataga tgtagaaata acaacatcat attcttcttg taaatcgatt ttttttaaat
    24721 cagcaatccg atagaaatga atttgttgga tgaagggaaa ataccttttc aatcgtagtt
    24781 ccaacatgct agtagaagct aatccacttg gcgaaaatcc tgcaatactg atattgcttt
    24841 ctttcggact tcgttccaat gaattcgcaa aatgaaggac catataagcg atctcttctt
    24901 cggataattt tttttcttga aaaatccgct ctactgcttt acggattgca ttcgcaactt
    24961 cctcgtattg aaccatgatt ttttctaaaa tcggattggt cagtgtctct tcttgtaaaa
    25021 cggctcttga aaacacacca gataaatgag tcaacagcat tttgtacaaa gtttcatctt
    25081 cataaaaatg gacattcgtc tcttcactta cttcttgaat cagttgtttt actaaataag
    25141 caagctgtgt atcgaacgtc tcttcaaaga aattctgatc aaatgagttc gtgaagtcat
    25201 ttaataatcc tgcaaaaaag acgatctcat ttactggata aagttgtttt gtctcagaag
    25261 cgatccgtga aaacaatcgt ttagctgtct gtagtaattc tttgtttgcc agactattat
    25321 atgtctcatt tgtcagtaaa tgatttttcg tcacacgctc catcgtcaat gtcagcatca
    25381 aaaccaaatg ttcaagcttt ctatcactta gattggtctt tttttggatc aattcttccc
    25441 cgattacttt gcgtgcaaga gatagatgtt gcggatcaac gaatcgaaaa aaataattca
    25501 gtgatgcatc gaaagaaata ctatgaaaaa gttcatattc attgatttct aattctaata
    25561 cattcgctgt cagaagccgg cggtattttt ccggtccttc gatctcgtat ccctggttcc
    25621 gaatgatttc caaaggcagt tggcgaatac tttcttccaa ctgtttgata tctgcataaa
    25681 aagtggtatt actgatgcga tatgattcca tgaaatgagc aagactcaac ggctctgtcg
    25741 tcagtaacaa ctcggctaaa atcgcgtgtt gtcgtttagc agtggataag tcattacttt
    25801 tcccatctgt attttctttt aattgctcta atacttcttc tgatgcctca atgacaaatg
    25861 agcctctagc tgcttttttc aaagaagcat ttaccgattt caatgagact tcaagattat
    25921 ctaattcgcg gtaaatagtc cgtttactga ccttcaataa attcatcatt ttttggatag
    25981 atacaggtga cgggctactg agaaattctg ctaataattg tttttctctg ggcgataaat
    26041 acatcttata tttctccctt cacgagtttt tcgtattcat tcgtagctac ccaatgttca
    26101 actagaacta gccgagggat cttttcttct agctgttcag ttacagctag ttctctttgt
    26161 gcgatataaa tcgtttcttc ttggattttg attttttcta ttgattcttt tgagataaca
    26221 aactgttttt tcgctttttt tgctaattga cttaaaatcg tcatgcccat agtttgtgag
    26281 ccgcgtctgt tgtttttata caaaaataca atattagcag gcgacgtttt ttgttctgtg
    26341 atatctgcta tctctttttt ttcttcccgc tttggaaatt ggatcgcttc ttttattcgt
    26401 gacagcaaga tatctgtttc caaaaagtga tccagtacat agatttgact ttgaggtact
    26461 ttttctcttg caagacccgc tagttcatgt tgcacgataa ccagctgatt tacttggtta
    26521 ttcaattcat agatagagct gtatccgaca gaaagatcga ttttttctgc tgctaatttc
    26581 tttttcagca gggcggctcc catagcactt gatcccatac ctgagtcgca agcaaaaatg
    26641 acggaatgaa tgatagatgg ttccaagatt tctccccttt ctttgaccgt tttgttctct
    26701 ttttctttat ttggtaattt caatagtatc gctgcacaaa tgaaggtcac aaatgtactg
    26761 attgctatcc ctatacttac cccagctagt tgtggataag gcgcattggc aaaaatcacg
    26821 agtagtgacc caggcgatac aggtgcagct aatcccgtat ctaaccattg aaatatcaac
    26881 gtgccgcttg ccccaccagc cattacagct aaaaatagat acggtcgcag caagacaaat
    26941 ggaaaataga tttcgtgaat cccgccaatc gcatggatca tcaatgctcc actcgcattt
    27001 actttttcct gtttttctgc aaaacaaagg atagaaagta agatccctaa accaggtcca
    27061 ggatttgcct ccatcaaaaa taaggaagag tacccgactg tttgtgagaa ttcgattcct
    27121 aaaggtgtca atacaccatg attgattgca ttattgaaaa atagtacttt tagaggttct
    27181 aagatcagac tgacaagtgg taacagattc attgtgacta gccaagacac aacggaggcg
    27241 agcaacaagt gaacactatt taaaactggt tcaataaaaa cgattccaat tatacaaata
    27301 acgctaccag tcagtcctac tagtaggttg ctgacaagca tttcatatcc agccttgaat
    27361 ttcacatgga atctagaaac aaattgttca tatacgatgc cggcaagcgg cccgataatc
    27421 atcgctccta cgatttgtgg agcattggaa cgagcgatca accctaaaga agcaattgca
    27481 ccggcgactg cccctttttg tccccctacc gatttcccgc ctgaataact gatcaagatg
    27541 ggtaaaagca gctggatcat ccaatggcat acctcttcta atgtggtgtg caaatcgcca
    27601 gacacacctt ttgttgctaa tgacaagatt ccccaagcga taaacagact tagatttggc
    27661 atgatcattt catttaagtg atgtccagct tttctcagtt gtttcataga cacgcgactg
    27721 ctgttgtttg ctttccattt gtattccatc tttttcacca gacttctatt tattgatgga
    27781 cggattttat gtagaaaaag cagcaaacga aaaaccgtct gctgctttca gctatctggg
    27841 aactatttgc ctggttcaga cgcaatgatt gctagttcac cttggaccgc ccactcagaa
    27901 acgccatttg gaataataca gcttgttcct ttttctaatg gatatgtttt tccgtcaacg
    27961 acaaggtctc ctgctccttc gattaccgtc atcaatgtat aaggggcttg ttttttgaaa
    28021 gatgtgattc ctttaatatc ccactcatat acattgaaaa actcagtttc taaataagtg
    28081 acgatcgatg aattcccttt tcggacttct ttgatttgta attctggtgt tttcgcaggg
    28141 acagttgtta catcgatcga ttgttggata tgcagctctc ttgtttgtcc ttgcgcatct
    28201 tttctgtcgt agtcatacac gcggtaagtc gtgtcagaac tttgctgtgt ttctaaaatc
    28261 atgattcctt taccaatagc atggatcgtt ccgcttggta cataaaagaa atcacctttt
    28321 ttaactggta ctttcttcaa aaggtcatcc cagcgcccat ctttgatcat ctcagctagt
    28381 tcttctcttg tctttgcatg atgaccatag atgatttctg caccaggttc agcatcgatg
    28441 atataccagc attcagtttt tcctaattca ccttcatgtt tcatcccata ggcatcatct
    28501 ggatgtactt gtacagatag atcatcttct gcgtctaaga tcttgatcaa taatggaaac
    28561 actggttctg aaggatggcc aaacaattcc ttgtgttctg accatagttc atctaatctt
    28621 ttccctttga attcgccatt ttctactaca ctgacaccat gaggatgcgc actgatcgcc
    28681 caatcttctc cgattttatc gttagggata tcaaaaccaa agactgaacg taaacgactt
    28741 cctccccaaa ttttttcttg aaaaactggt tttaaaaaca taggttcctg cataattcaa
    28801 actcctctca tttttcatag ctattatagc attatttcgg taataccgct ttctctttca
    28861 aaaagttttc aacaatttca tctcgacata tgagatactc cgcacgatga tcgacaggat
    28921 gtacgcgatt tgacaaaaat atcaacgctt cttgtgtttt tatatcaatc aagaaaaacg
    28981 tacctgtata accagtatga aaaagaatcg catgaccgtc ttctacatcc ttttttaagt
    29041 cccatcctaa agaacgatag ccattttttt tcggtgtttg atcttgcagt aaagaatcaa
    29101 tcgtctcttc ttctaaaaaa cgaccattac ggatttttcc cttgttcaaa tacatttgga
    29161 caaaagtaat gctgtctttc aaatcaataa agagacccgc gttacctgca tgttcagcca
    29221 aaactcttgc tttcggatca tgagtttgtc cacgcaaaac ctcacccgaa ggcagttgtt
    29281 ctgtgggaac gatttttttg gaaggaaaag gaagaaataa gctgttcatc attcctaatg
    29341 gttccaatac ttgttcttga aagatttcca ctgcagtttc ttggtaaatt tcctctagca
    29401 tcaacccaag aagaatcgta ccggcatctg tatattttac ttcttttcct agttgctcac
    29461 ctggttctag cttcaaataa gcggcgatca attcttcctg agatagctta tctctgtttg
    29521 gtatccatgt tttgatatca gaagtatgtg tcagtaaatg acgcaaagtg attgtctgat
    29581 tttcaaattc aggaagatat ttatgaagcg gttgatcgag ttctatcttt ccatcttcta
    29641 atagacgtaa aactaatgaa gtggttgcaa cgactttcgt cagtgaggct gcatcaaaca
    29701 acagatcttc tgtcatcagc tgcttttcag gaacgacttg ggcaaagccg gctgtaaatg
    29761 tttctacttg attttctttt atgaaggcga ctgctacacc tggataaaat ccttcttcca
    29821 tctttttcaa aataagctgt ttcgtgtgtg gatacatctt tttctcctgc ttttctatca
    29881 gattcttaca gtccaagctt aaagcttaat ctatgaggaa tcaacctttt ttacacaaaa
    29941 aaactgcgcg aaaaaccgcg cagtttcccc cggtatacta ttttctcttt ctatctctag
    30001 aataagcgat agtatgaccg ttcataaaat gacgagacat gcgacaatcg ttaagaaaga
    30061 aaaagttaag cgtcgcatcg aatgtcagtt ttattctttt cttttggctc actgacgaac
    30121 caccattcga ttcctatagc atcatagatt gtgagtactg tgtgcttttt gtctaagaag
    30181 aaatcaactg atagttgctg caagtgagca atcagtcctt ccatgctttc agtatcgatc
    30241 atgaatttca gaaaatcaag tccaatcact tcatctgtag gcaaatcaat tgcgcctccg
    30301 ctagcttctg tcaaaccaac atggaaatct ccttcattca atacatggat gccctcttct
    30361 tcattatgta ccttcaaacc aagtacatga ttaagaaatt ggcgttcttt tgttaagtta
    30421 gtaacattta aatggatttt ttcaaaataa gaacctgttg atagtttact gaatttttct
    30481 tcagattttc ttaataatgc ttcttgatcc attgtttctg gaatttctct ttggtcgttc
    30541 ttattctctt catggtaaat ttccaattca ttctcttctg ggtctacgag caaaatcccc
    30601 atacgtccat gatcttctaa agcttctttg attggataat tcattttacg gatacgacaa
    30661 aggatatctg ccatttcttc tgtgctaggg ataatcaatg aaaaacggtt cagctttttg
    30721 atcaccccca tgtgatcatt cgctctagga ctttcttcca gcagcaacat ttcacttcca
    30781 gcttcttctg ttcctaaaat ggctaattcg ttttcttctc ttttcaaatc aaagccaaca
    30841 acgttcttgt aaaaatcaat cattttatct cggtctttca cccgaatggc tacacttttt
    30901 aaataggtgg cagcccccaa ttgaaaatct ttcatatttc ttgctcccct ctacgtcaag
    30961 ctttatttta acgatggatt cttcttcttt caagcaataa ctttgatttt gttaaaaaaa
    31021 atgtgagaaa attcgcattt tgcttgtttt ttgtaaatta atgagtaaaa actcttataa
    31081 aaagaggttg tgaaaaagct gttctgcatc aagtaataag aacagccaaa caaaaatagc
    31141 tcctcctatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt gaacaccgtt
    31201 taaggaagag gttgtgaaaa agtcgcttag tttcgagtag taagaaaaat tttgtgaaaa
    31261 tggctttcta catttttgca taaatttggc ttaataccga gaaactggct tttgaacacc
    31321 gtttaccaag aggttgtgag cctgccgttt aactccgagt cacaaggagc aatttcaaaa
    31381 aacagcttct caggtttgtt gactattgtg acttgtgacc gaggagttgg caggagaaca
    31441 ccgtttaagg aagaggttgt aaaatcagcg gtttgacctg agtattagag atacaaaacg
    31501 gaaaaatagc ttctcatatt ttgccgtttt ttgagctaat accgaaggac agctgattga
    31561 acaccgttta ttcggaaaaa gggactgtga cacgactttt gtcacagccc ctaaaaaaaa
    31621 taataaattt gtactattct atgaagcttc ttcttcagaa aactgactgt tataaagatc
    31681 tgcatagaat ccgtcttttt ccatcaatgt atcgtggttt cctgtttcta cgatcgaacc
    31741 atgattcatc acaataatgt tgtctgcatc gcgtattgta gacaagcggt gagcaacaac
    31801 gaaacttgtc cggttttcca ataggcggtt catcgctttt tgaatcagta tttctgttcg
    31861 tgtatctaca ctagatgttg cttcatcaag gatcaggaca tctggatttg ctaagaatgc
    31921 ccgcgcaatt gtgattagct gacgctgccc ttgcgaaata ttgcttgcat cctcgtttaa
    31981 tacagtgtta tatccttctg gcaatttacg aacaaaatcg tctacgtgag cagctttagc
    32041 agcctcaatg atttgttcgt ctgttgcatc gtcgttccca tatttgatat tgtcatagat
    32101 acttcctgta aacagccaag tatcttgcag taccattgaa aactgtgcgc gcaattcatc
    32161 acgtgaaaga tcacgggtat ctacaccatc gtaacgaata ctacctccag aaacatcgta
    32221 gaaccgttca agaaggttga tcagtgtcgt cttccctgca ccagtcggac ctacaatagc
    32281 gaccatctca ccaggtttga catccaagtt aaaatcagtc attagcaagt tatctggtgt
    32341 gtagccgaat tgaacatttt caaaacgaac tttgtatggt gtatcttttt cttctggaag
    32401 gccagatttt tcattggaca tctcttcttc atccaacact tcaaataccc gttcggctga
    32461 agcaactgtt gattggatag tgttcatcaa acttgcaagt tgagtgattg gttgagagaa
    32521 ctgtgttgta tattgcaaga acgcttgaac atcccctaat gtcatgttcc catttgcaac
    32581 ttttactccg cctagtacag cgacgaacac atagcctaga tttttaacga aattcatcaa
    32641 cggcatgatg attgccgaaa taaattgagc tttccaacca gcggcataca gattctgatt
    32701 ttctttttca aatacttcct ggtctttttc agtatgattg aatgttttaa caatggtatg
    32761 accaccatat gtttcttcta cttggttatt caataaacct aaactttttt gctgatctgc
    32821 gaaaaacact tgtgatttcg gcgctacgat catcacgatg atcaagctta aagggatcat
    32881 caataacgcg actaacgtca attgccagct gattgaaagc atcatcacga tgaccccaat
    32941 caatgtgact gcactagtta caaattgagt caaattttgc tgcagcgtgc ttgcgatgtt
    33001 atccatatca ttgattgctc gagacatgat atccccattt gtatgtgtat cataatagga
    33061 aatcggtaca cgattcattt tggcttccaa atcatgacgt aattcataaa ctgttcgttg
    33121 ggaaacacgt gtcataataa attgctgtaa aaagttgaat acagcagaaa tcaaatacat
    33181 cacgattact gtcgcaatga tctgcgcgat tttatcaaag tctatcggaa attttgttac
    33241 ttgttggcct tgttgcatct gttgagcgcc agccattaca cctttgaata tttctgtggt
    33301 tgctttcccc aagacctttg gtgtttggat ttggaagatc acggctgcta ttgctagtac
    33361 aaatacagcg atgatcgcaa ccatacgttt agacatatag ccaaacaatc ggcgtaccgt
    33421 tccccagaaa tttttaggct tttcaccttt tacaccgaga tttctgccag gtccaccatg
    33481 tcctggaccg cctgctggtc catgagaggg acgtgtgttt gttttactca tgctaagtcc
    33541 tcctctctta gttgtgaatg aacgatttct tgataagttt cgtttgttgc taatagttct
    33601 tcatgtgttc ctttgccaac cagttttcct tcatctagca caaggatcat atctgcatcc
    33661 attactgtac ttactcgttg agcaactaaa acggtgatac tttctttcat attcgttttc
    33721 aatgcttggc gaagattggc atccgttttg aaatccaacg ctgagaagga atcatcaaac
    33781 acatagatat ctgctgtttt gactaatgcc cgtgcaattg ccagacgctg tctttgtcca
    33841 ccagagaagt ttcctcctcc ttgttccaca tggctatcta agccatcttc catctctgaa
    33901 acaaaatctt tagcttgtgc gatttctaat gctttccaaa tcatttcatc tgttgcatcc
    33961 ggcttaccat actgcatatt gtctcggatc gttccagaga acagaactgc tttttgagga
    34021 acaaagccca ttaattctcg caaattatat tgagtcatgt cactaatgtc cgttccattt
    34081 atttggatcg aaccagattc gatgtcatat aatcgaggga tcaaattgac taaggtcgtt
    34141 ttacctgaac cagtcccccc tatgattgcg acgatctcgc ccgacttggc ttggaaatca
    34201 atatcttcca atgcaagttt ttctgctcca tgatatcgat aattgacatg attgaaggcc
    34261 aatgttgcgt tttttccagc aaaagaaact gttttaggat tttcagggtc atggataccg
    34321 attttttcat ccaacacttc attaatacgg tcagctgaag cttgtgcacg aggcaccatg
    34381 ataaaaatag cggaaagcat cataaagctg atcaagatct gcatcgcata agtcataaat
    34441 gcaatcagat taccgacttc caaagtcata tctgctatgt agtgtccacc aaaccaagtg
    34501 attgctacgt ttgtaccgct catgatcaat gtcatcattg gcatcatcaa tgcgacgatc
    34561 gtattaactt tgattgccgt atttgtataa tctttattcg ctaaatcaaa acgattttct
    34621 tcaaagcgtg tcttatcgaa tgctcgaatc actctgaccc ctgtcaatcc ttctcgaaat
    34681 accaggttta aacggtcagt tttcttttgc atacttttga ataatggaac agcaaagtac
    34741 atgattccgc cgatcaaaat aatcattact gggatgacaa ctaagaagat ttttgtcaac
    34801 tgatgatctt tttgataagc aagtatgctc gcgccaatca acgtgattgg tgcattgatc
    34861 atcatacgta aaaacatttg tgtaaccatt tggatttgag tcacatcatt ggtcgttctt
    34921 gtgatcaaag aggccgttcc atatttatca aacgcatctt ttgtcagact ctctgatttt
    34981 ttatagacat ccgtacgcaa ttgcttaccc agtttctgtg attcacgagt agcaaaaaat
    35041 gtatttgcga tagcagctaa gatactgatc aaagaaaagc cgatcatcac aaaacctgta
    35101 tgccaaatat aatcgacatc tccttgcgct acccctttat caataatatt ggaggtaagt
    35161 gtcggcaaat ataaatctgc cagtacttgg atcaccatga aaactgcagc tgcaattgct
    35221 gaagtcaagg agatccgttt tacgattttt agcatctagt tcccttcttt ctatttatat
    35281 actccgtttt tcagccaaga aagtttcaaa ttgaattcac tgattgcttg ctctactgag
    35341 taatcttcag atgttgaaaa taagcgatac gaatgcgcaa ttgttgtaaa tacagtattc
    35401 atcactaatt ggatcagtaa ttccaattca tgatcatttt ttactttcag cttcgtccga
    35461 tcgatcaatt caagcaactc ttttgcatct tgtttgtgtt ctttatgtgg atcgtgaggc
    35521 gttttttggc gactcgtatg aggagcgact tttcttgagg cacggtaatc catgttcata
    35581 aacaagttac gataaaaaga agcattctcg ccttccaaaa tctcataaat cattttagaa
    35641 aaataggatt cgaatccttt gaatagatca ccatcggatt ctttcattag ttgtaacata
    35701 tctttagagc tgtcacgtct cattgtctca aagtaataaa aataaagatc ctctttatct
    35761 tcaaaatact gatagaaact gcctctaggg atatccgcta atttgacgat attagcaatc
    35821 gaagcttctt ttaagggtac tctagaaaac tcgattctcg cagcctcgat tagtcttttt
    35881 tgtttttctt taggcaaatg gaaaaatgtc tgtgtcggca tatggattct cctttctttc
    35941 aagatgacac attgtcatat gacgctatgt cattataaca cctcctacct gttttgcaag
    36001 acatttgcat agtttttttc attatcatcc gatacattaa aaaagtgata agaggaatcc
    36061 ttccatttga attaatgaga gttgcccatt aaaatagcca catactaaac aaaggatgag
    36121 ataaatggcc caatcaatta gtgtccaaga cttcaataaa ttgcctctcg atgctgacag
    36181 catcgtttta gatacaagaa ataccttaga ttacacaaaa aaccacttaa gccactcgct
    36241 ttctatcccc gctgctatct tgccaagaca attaaaaaat ctagatccaa agaaacttta
    36301 ttatattcta agctatacag gcaggcgatc agaagcatta gccgatcaac ttactgctaa
    36361 tggttttcgt gctgtttata tcattggtgg gatggaagct cttcagtcga gtgttgcttg
    36421 aatattgctt gttagattca aaagaggctg tgacaaaaaa tcgtgtcaca gcctctttta
    36481 taccctgtct ttttttagtt atttattctt caaccttatc aatatgactt gatagccttt
    36541 tctttttcga gtaaactgat tttgttagtc atagttataa caatcatagc aataaaagga
    36601 gaaggtaagc atatggcatc tgttttagta gtcaaaggtc atcccctaac tgcagaagaa
    36661 tcgcgcacag ttaaggcatt gacgtctttt ttaacaagtt ataaagaaaa tcatccagat
    36721 gatgaagtga ctgttttgga actttatcgc gatgatattc cggaaatcga tgaagaatta
    36781 ttatcaggtt gggaagcatt acgtgctggt gcagaattca cttccttatc agaaagtcaa
    36841 caacaaaaaa ttgctcgttt caatgaattg actgatcagt tccttgctgc tgataaaatc
    36901 gttatcgcaa acgccttatg gaatctaaat atcccaacaa aattaaaagc ttggttcgat
    36961 accgtcaatg tagctggaaa aacgttccgc tatacagaaa acgggccaga aggattagtg
    37021 acaggtaaaa aagcactgca catccaatct aatggcggtg tatacaacgg acaagacttt
    37081 gcctctcaat atgtaaaagg tattttaaac tttgtaggca tcgatcaagt agatcagcta
    37141 ttcatcgaag gaatcgatta tgaccctgac cgtgcagatg agttgatgca aaatgcatta
    37201 gataaagctg ctgctttagg taaatcattt taaaaattga gataaatgca tacaaaagag
    37261 acagaggaaa aaatcttcaa agcagcattt gtgccttgga tattttccca tctgtctctt
    37321 tttgttattc tttctgcttt ttcaacggat tttcttcata aagagaagag attgttgtgt
    37381 accattcaaa aacatgagta atcactactt tagctgctgc ataaacagga atccccaaga
    37441 tcacaccgat gattccgaac aatttcccag aagtcagcag aactaataaa atcgttactg
    37501 gatggattgc taactgattc cctagtacta atggagagat caagcgtcct tctattgtct
    37561 gctctactgc aaaaacgatc agtactttga tgatcatgaa tggacctgct acaatggcta
    37621 aaaagatagc cggaatcatc gctaaaaatg atcctaagta agggatcagg tttaaaaaac
    37681 ccgcaataat ccccaacgtg acagcatact ctaaaccaat gaccgcaaaa ccaatcataa
    37741 acatgattgc cacagcaaaa gccacagtta gttgtcctct tatataagaa gaaacttgac
    37801 tattcatttc actcagcaca ttcaacgtcg gtttttgcat gcgtgtcggt aaaaatttga
    37861 caaaataagg tgctaattgt ttaccgtctt tcaatagata aaataagata aacggcatcg
    37921 tcagcagagc cactaaaata gtcgctaccg cgccgacaaa acttccaaga ctctgaactg
    37981 tactcttaga tatatcttgg atcatatcgc ctgccgaatt catgaactta tcacctgctg
    38041 tttccaactg ttcgcgaaat tggttgaaca gcgggtcccg caaaatctcg gttaatttat
    38101 tatcaatcgt atctacatat tgagggaagt tactgatgaa gctcactgtt tgttcctgaa
    38161 tcttagggat gatcactacg ctcccccatg cgattagtgc aacaaccaaa acaaacagcc
    38221 caatgatact gtaaagcctt ggtacttttc gcttttcaaa gtaatcaact accggattca
    38281 tcaagtaata caagattcct gctaaaatga taggcagacc tacgattgct aaaaattgcc
    38341 agattggtgc aaacaaatat gaaacttttg taaataaaaa gacgatcagc aatattaaca
    38401 atacaacaag taaggctgtg actacttgat tatttaaaaa ccagcgccaa aaccacgata
    38461 ccggtctgtt cttcttattt tttccttcca taattcctcc tgttagttat aaaaaatttg
    38521 gcttcccaaa ccaatagcag gcagtctatg cggatgaaga tagattccat ttgcaatttg
    38581 tccttcttct ggacaagcat caaaaatcaa tgatacgtga gctactgtct ttaaattccc
    38641 attggccatc ggaccgacat aatcgatcgt atatacctga tcatcaaaag caattgttcc
    38701 gcctgctttc aaagaaaaag gttcgtcttt tgatacttct tgaatcaccg aatattttcg
    38761 taaggcagct gttgctgttt tatcgaacaa aatcagcatg ggttcttcct tgtttattgc
    38821 ttctgtccca atttctgtaa tctttgcctg aatcattgca ctctctcctt taattgcttt
    38881 caaaaacagt ataacacatt ctcattcttt agccgtgtgg aataattatg tgatagaata
    38941 aaaacgaaaa ctatttcatt acaattagga ggaatcagct catatgatcg aaaagataat
    39001 tttaggaacc tatacgcgtc gcgaaagtaa aggaatctat acgatcgacc ttgatacaga
    39061 aaaagaagaa ttagccaacc tttcattagc tgttgaagaa aatagcccta cctatgtagc
    39121 aaaaagtaaa gctggcaatc tttacacagt tactagtgtg gatggattag gtggtgccgg
    39181 tgcgtacgat aaggattttc attttttgaa tgcggtaaca gagtctggcg ctcctttgtg
    39241 ttatgtagcg gttgatgaag cacgtcaatt actatacggg gcgaactatc ataaaggtga
    39301 agtaaatgtc taccatatat taggagatgg cgaactgaaa gcagcagatt caatattcca
    39361 tcaagaagcg acaggtcctc ataaaaacca agatcatgcg catgtccact atacggatct
    39421 gactcctgac caacgattag tcgtatgtga tcttggtaca gatcgtgtgt atacttatga
    39481 tgtgtccgaa aacggcaaat tgaatcttgt aactaccttc actgcagagc caggaacagg
    39541 ccctcgccat cttgtctttc atccaaatgg acaattcgct tatttatttg gtgaattaga
    39601 cagcacagtc cgcgtgcttt cttataataa agaaacaggt gcttttgaag aattacagaa
    39661 aatatcgacc ttgccagaag aattcgacgg tgaaaatgga ggggcagcta tccgcatttc
    39721 aaatgatggc aagttccttt atgcttctaa ccgaggacat aactcgattg ctgtattcgc
    39781 agtttttgaa gatggttccc acattcaaaa caagcaaatc atctcaacag aaggggattt
    39841 tccccgcgat ttcgcactag atccaagcaa taattttgtc gtgtgtgcca atcagaacac
    39901 cgataatctg acattatatc gccgtcatcc tgaaacagga ttactggaat tactgaaaaa
    39961 agatgtctat gtaccagaat gtgtctgtgt ttattttgat cgttagtaag aaaaggagaa
    40021 tgaaatgaat cagcaagacg tattagacag actacgggaa gaactaaata ttcctttctt
    40081 tgatgggaaa atcgaagata aagagtatag cgaagaggaa tatcagaaac tcaaagccga
    40141 tttacagaac tactttgaac aatatgtaca gaatgtagaa aactaatcag ctttccatac
    40201 tatctgaaaa gaacatcagg acaagacttt tccgtctatc ctgatgttct tttctacaaa
    40261 taaaaaaccg ccgagatggt tagactcgac ggaaaaggag tatttactcg attttgagta
    40321 agtacaaggt tgttgttggt tataggtatg tcattatcat aaaggatttt gatcactcta
    40381 tcataaaggt aatattacat ttatattaga taactctagt ttttattgtt atcaaagatt
    40441 tcttttattt tttcaacaac aaattagtca ttttttggat ggcagctgtc aattggtctg
    40501 tcgcttggtc ttgttttgct tcagatgtat tgaatagatc ttcatctacc cctgattcaa
    40561 gatcgatact ttggatcatt tcttcacagc ctgctacata tgaaagataa gctttttcaa
    40621 aatttttatg aatgcccatt gcttttgctg gtggacgcaa agtactgact ttcataagca
    40681 ttgcccgata tttttttgtt ccttcttcaa ataacaggat gatctcttca agagtcgatt
    40741 tgtccagttc acttgttttt ccttcatcaa ttgcgtttct gatcacttca tatttcggat
    40801 gcattccttc tccgattgct tctgtttcct tgacgatttg atcgatggac tgcacataat
    40861 agccaatatt cggtaacatg ttcgttttcc tactttctcc attgttgtta ttatttattc
    40921 tacttgtttt tatcctcaaa tgacaagtac ttgaaaaaat ttaatggttt ctatacagct
    40981 cgccattctt attcgtgctc aaagaaacca tctgctaaac tatattgttt ccctgcgaca
    41041 atgtcatatt ggatcaattg atattcgtcg taaatctctt gttgcgagcg attgaattgc
    41101 gcttctttat accactgtcc gttttgatag tacagttctc gttcaaacgc cggaagttcc
    41161 tgttctaatg ataaaagcag ctgataaaag ccatttgttg tctgattcgt ctgttccatt
    41221 agattcgctg caaaataaaa cggactggtg attgcatcgt gagtcgtttg cttttctaat
    41281 tctcctttac tgtcgaacat aagaaattgt gtttcatgca atgtttgttt gtcatttttt
    41341 gcttggatcg tatctgaata gatccctgga agatgatccc cccaaaaaac aactaaagtc
    41401 cttctagaaa gattcttcaa ttcttccgtg aactgcttca gtgccgtgct gctataggaa
    41461 atatcttgca gataattctc taagtcaagt gtcccacttc catctgaagt ttttgcagga
    41521 tagtctagat tatcatactt tccatcgtag ggcatatgtg tctgcatcgt gactaaatga
    41581 atgaactgag gtgttttttc ttccttgaga agtgtcatta cttctttgta agctgactca
    41641 tcagaaatat atggattatt ctctatcgta tccgtatagg tcatcgtacg ctcgctgatg
    41701 aactggtcaa atcctaatgt ctgataaaca tcctctcttt tatacatcga agtattgtat
    41761 ggatggatcg ctgtcgtctg gtatgattgt gcgtttaacg ctgaaacgat agaaggaagc
    41821 tgatccattt ttggtacaag catcgtgtaa ggggtggtga gttgcgcatt aaataacgcc
    41881 atagaaaaac cagtcaatgc ttcaaattcg atatttgctg ttccaccgcc atagttttga
    41941 gaaagcatat tgccactata tgtttgatca gctacttcat aataatctgc caatggctca
    42001 ccagatactt ctacgccgtt taatcgacta ggatctgaga aactttcact catcacgaac
    42061 acgatgttcg gcgactcttc ctctacagtt ttattttttt catcggctag cttctggtat
    42121 ttttcagtga tttctttgat cttttctttc gaatatcctt caggttcatc cattggttct
    42181 accttcagat tgtacaaaaa cccaccgata aaaccagtgt tatagtagtt cattttctgg
    42241 ctgtaaggta tccatagtgc cgttttatta tatgcttttc gcaacaaatt atcgggattg
    42301 ttgaaattac tgatataccc tagcagtcca attgccacaa gcagtgtcgt taatcggatc
    42361 aattggaatc gccttccttt tttcaggctt cgaaacatgt accagcaaaa caatcctatc
    42421 acggttcctg cggcagctaa gatcactgta aacagcatag tgccggtcat atctttcagc
    42481 aatccgattt cagtgatcat cttcaaatcg tctggataaa tcggttcttg ccgataactc
    42541 attttcatat aatttgcaaa acctaataca ccgatggcta cgacatacgt aagggaacct
    42601 aaagggacag atcctgctag tgcaatcaga aaaataagaa taatcagcaa gacgagacag
    42661 gctaagaaga atttttccgt atgccaagaa aaagcaaatt tcaacgcaag gtctacggat
    42721 agatcatttt gacaccactg caaaaataaa ttactaaaaa caacaagaaa aaagatactg
    42781 atgattgctg ctgttccttt taaaatcttt ttctgtttgg tcgaaagtct gcttatatat
    42841 cttttcaaat gcttgctcct tcatttctat caataaaaga atgttgcttc tcttatttta
    42901 gttgtacgaa attcgaaagt aaagaacgga aagaagaatt aatgaaatcc aaatattctc
    42961 tttctttatg gcctttctgg tttctttaat aagcggacct ttcgtactcc agaaattttt
    43021 tctagctgtt gtttcacttg attgagctgt tcaaaatcag tactgtctat gtcaatcaat
    43081 atgtagacat gctcattctt atggttacgc gatatatctg cgatcgctag ctcgttctca
    43141 ttcagcagag aaaatatctc tgctaatata ttggttttct tttgataaaa tatagtgaat
    43201 cgaaacggcg cttggaaaaa cattcgtgcg gatggataat tgaccgcttc tctaaccgta
    43261 ccgaacaaaa gataatcttt taaggcttca actgccaaac gtccgctgtc tgccaaagct
    43321 tcctgagtcg atccccctag atgaggcaac ataagtatac ggtcattttc taagaactct
    43381 tcttctggaa aatcagtcaa gtatttagcc agatgttgtt tttccaatgc ttgcataaca
    43441 gcatatttat caacaatttc acttcttcct acattgataa gtacagcatc ttttttcatt
    43501 ttttctatat tcgcctgatc gatcaatcct tttgtatctt ctgtcaacgg cagcatgact
    43561 acgatgtaat ccgaggtaga aagaactgtt tctaaatctg cttgccgaac ataatccaac
    43621 tgggcatcgc ggatagaata acccaagaca tccatcccta agctatagca acttagcgct
    43681 acttttgtgc ctatcgctcc aagaccaaga agaccgatgg ttttgccttg caattctcgt
    43741 cccacataag cacttctttt gttttctgcc tgttctaaaa tattaggacc tgttagtgtt
    43801 tgtaccatac gacttgcttc gattatcgga cgggaagata agagcaaaca gcacaaaaca
    43861 agctctttta ctgcattagc atttacacct ggtgtattca tgacgatcgt cccgttttca
    43921 gaagcttttt caacgttgat cgtgttcacc ccaacaccag ctcttgaaat tgctagcaaa
    43981 tctggtgtaa gccattcatc tttcactttc gtactacgaa taactattcc ttcggctggc
    44041 aaatcatcta ttattttgaa atattcccct tgtaactttt ctatctcttg ttctggaata
    44101 gcatcaatca aatgaacgtg atacataaaa ccccctcctt actaacaagt atctcgaaat
    44161 ttctgcaatt agacaactgt ttaccatttg acttcctaga agcaaaaaga gcagttagag
    44221 cagagtaggc tgagtgataa aaataaggct atgacaagtt actgccataa gccttatttt
    44281 cgagagcaaa atattgaggg tgagatatct tgataaatgg tgttccgtta gctgacattg
    44341 ataataagac taaatttcaa aaaagttgaa aagcaaattt cgtcaatctc ttcttatttc
    44401 ttaaaactaa gtggcttttt cacaacctct ttttatctaa taaccactgt ttccaactca
    44461 actaattttg cccacgtttc aatttgatct gttgttagat ttagcgagac aacggtatga
    44521 tgaccgccac ctgcctgaat ccatgaatgg acaccttcat ggaaattcgg tttagttttc
    44581 cataacacac gtgcaacagg taaattaggt gcttcagtcg ttggttcaaa ggcttctact
    44641 tcattgatca gcagtttata atgcgtaccg aaatctgcca tcgatacgac taccccttct
    44701 cctgctttcc catcaaatac aagacgcgcc ggatcttctc tatctcccat tgataaagga
    44761 gaaacaacga ttcttggttt cgtcgcagca agcgtcggat ctacttccat catatgggat
    44821 tccaagattg cttcttggcc actagctagc tcgtaagtat aatcttccat gaatcctgta
    44881 tctttccctc gagccatgat tttcagcaac cgatcaatcg ctgcagtttt ccagtcgcct
    44941 tccccagcaa aaccgtatcc ttctgccatc aaacgttgta cagctagtcc aggtagttgt
    45001 ttcattccat acaggtcttc aaaattagtc gtaaaagcgt tatagcctcc ttcttctaag
    45061 aaacggcgaa taccgatttc ctgctgagct tgtactttta catgttcctc ccatgtcttt
    45121 tcttcataat caccatagtc gaaggtatac aattccttgt actcttcaaa taattgatcg
    45181 acttcttcgt ctgatacgcg atcgattact tgtacaagat ctccgatacc aaaataatca
    45241 acagtccaac caaattgaat ctgtgcttcc actttgtctc cttctgtgac acccacgttt
    45301 ctcatgttgt caccaaaacg agccactttt attccaaaac tttcattata ggctactgca
    45361 acatccatcc aatcagcaat ctctttttgg atctcaggtc gtttccagta gccaacgacg
    45421 attttatttt gtttatttaa tcgagcattg atgaacccat attcgcggtc accatgagcg
    45481 gattgattta aattcatgaa atccatatcg atcgttttcc aaggaatgct ttcgttatat
    45541 tgcgtagcta aatgaagcaa tggtttttgt agaagctttg tcccgcggat ccacattttt
    45601 gctggtgaga aagtatgcat ccaagtgatc acacctgcta cttcttctcg ataattgacc
    45661 tctttcatga tttttgtgat cgtatcagca gtaactgcta attcttgaag gcggattgga
    45721 taaggcaatt ggccattttc attcagttca tcgaccattt cttgtgcatg tttctttact
    45781 tctcgcagtg cttcttcccc atataaatgc tgtgatccta caacaaacca aaattctttt
    45841 tctccaatat tcaacataat tagccactcc ttttatttgt tttgtccgta atacgcattt
    45901 tttccgtgtt ttcgtaaata atgtttatct aaaatccgtt gaggcagctc ttctgaaaat
    45961 gaattgagct gtctggtaaa taaattcatc ttgcatactt catctagtac gactgcgttc
    46021 attaccgcac tttgcgcatc tttcccccaa gtaaatggac cgtgtccgtg taaaagtacc
    46081 cctggaattg ctaagggatc aattttgcgt tctttgaatg tttctatgat aacttttcct
    46141 gtttcatatt cgtacccgct gtcgatttcc tgttgtgtca aaaatcgagc acaaggtacc
    46201 gatccataaa aagtatctgc atgcgtcgtc cccatcgcag gaacatccaa tcctgcttgt
    46261 gcccagatcg tcgcccatgt cgagtgagta tggacgattc ctccgatttg aggaaatgaa
    46321 cgatataaaa cagcatgagt aggcgtatcc gaagagggat tgagtttacc ttcaatgata
    46381 tgattgtcca agtccacaac gaccatgtcg tctgctgtca tctcttcata actgactccg
    46441 ctaggtttga taacgaaata tcgtgtttca ggatcaaaag cgctcacatt tccccatgta
    46501 tatttgacga gtccgtgttt aggcaagtca agatttgctt ggtatacttc ttctttcagt
    46561 tgttccagca ttttctatca ctcctttttc ttgcaattga tgaatcgctg ttttttcaat
    46621 gtcgatcccc tcacggtaac gtctcataaa tatctgatag cctgctattt cttctgctga
    46681 aggtgtgatg gtgattcctt ggtcttgtcc aaacacgtct tctgccaaga agttttctaa
    46741 agacttccca tgtcgatcca tcatataggc tgctaataac gcgattcccc acgctccacc
    46801 ttctccggct gtgtccatca ctgtaactgg tgcctccata gcagatgcta gaatccgctg
    46861 tccaacttct ggtgttttaa aaatcccccc atgccccact aaacgatcaa tttggacatt
    46921 ctcagatttc aatatatcca ttccgattct cattgcacca aaagcactgg ctaaatggat
    46981 ccgcatgaaa ttcgctaaat caaaacggct acctggtgta cgtacaaata atggccttcc
    47041 ttcatttact cctgtaatgt tttcaccgga aaaataacca taagaaagta atcccccgca
    47101 atccgtatcg ccttctagcg ctttgttgaa taataccgtg aataatttat ctctgcttat
    47161 ctccataccc aaagaattgg taaattcttc aaaaatactg acccaagcat tgatatccga
    47221 agagcagtta ttcgtatgaa ccatgcctac tagttctcct gaaggtgtag tgaccatgtc
    47281 gatttccgga tggacatttt ctagttcttt ttctaatacg atcatagcga aggcggaagt
    47341 accagccgaa atgttccctg tccctttggc tacactgttc gtagcgacca taccggttcc
    47401 tgcatcccct tctggcggac aaaccgggat tcctggtctc aaatttcccg tagggtcgat
    47461 cagataagcc cctgattcgc ttaacttgcc agcttcttct cctgctacta gaactttagg
    47521 caaaatgtga tccaagtcaa tcgctacgtg cgctttttcg attaactgac caaaaatatt
    47581 tttcatttct tgatggtagg ttttcgttga aggatcgata ggaaacatgc ctgatgcatc
    47641 tcctacgcct agtactttct caccagtaag ctgccaatgg acataacctg caagcgttgt
    47701 caaaaaatta atttctttca catgttcttc tttattcaaa atagattgat acaaatgagc
    47761 gatactccat ctctgcggaa tattgtattg aaatttttcc gttaatattc gttctgcttc
    47821 ttcagtgatt gtatttcgcc aagtacgaaa tggtactagt aactgttcct tgctatcaaa
    47881 tgctagataa ccgtgcatca ttgcactgaa cccaatcgac ccgattgtcg taagattctc
    47941 gccatagatt tcgaatactt ctgctgccat ttttcggtaa ctgacgcgca gtcctttcca
    48001 aatatcttct aatgaatagg tccagatacc attttctagt ttgttttccc actcataact
    48061 accagcggca ataggagtaa aagatgaatc aatcaataca gctttgatcc tagttgatcc
    48121 aaactcaatc cctaaagatg tacgaccatc tatgacatct aacatacttt gcgcatttac
    48181 ttccttcata atgatttaac tccctccttc tcgtcaaacc tgatcaaacc gtctttttag
    48241 tagcgcttac aatgattatt gtatactttt gtacgtacat gtcaagcagt tatttacaag
    48301 aatacaataa aaaacacttg ttttgtacgt acataagtta taaaatgaaa ttgcttacaa
    48361 aatctgatca accgccttct cgaaattcta gaaggagaga atacttatga aaaaagaaaa
    48421 aaaagtttcc tcatcgttta tttatttttt tggcgcattt ggcggcattc tatttggata
    48481 tgatattggt gttatgaccg gcgccttacc ttttttacag catgattggg gtttggcagg
    48541 taaagcttct ttgataggat ggatcacgtc ttcggtcatg ttaggtgcga tccttggcgg
    48601 ctctttatct ggtcttcttt ctgataagct tggacgacga aaaatgattc ttctttctgc
    48661 acttattttt atggctggtt ctgtcctttc tgcatctgcc cctcacaatg gttcttattt
    48721 tctgattgca gcaaggattc ttctaggttt agctgttggc gctgcatctg ccctagttcc
    48781 tgcttatatg tctgaaatgg ctcctgctag actaagggga agattgtcag ggatcaatca
    48841 agtgatgatt gcttctggca tgctcctttc ctatgttgca gattatttgc taaaagggtt
    48901 gcctgaaacg atggcttggc gcgtgatgtt agggctcgct gctgtccccg cgctgatttt
    48961 atttttcggt gttttagctc taccagagtc accgcgtttt cttatgcaat ctggccgctt
    49021 ggaagaggca aaaagagtat tgaactatat tcggacacca aaagaagctg aacaggaatt
    49081 cgaacaaatc cagctgaatg tcaaacaaga aaagacaacc ggaacttctt ggcatacttt
    49141 gtttttagaa aaatatcgtt ccttagtcat tgccggtatt ggtgtagcag tattccagca
    49201 atttcaagga gctaatgcta ttttttacta tattccgctg atcgtagaaa aagcgactgg
    49261 gcatgcagcg agcgatgcat tgatgtggcc gatcatccaa ggaatcattt tagttgctgg
    49321 ttctttgctc tttttggtta ttgcagacaa gttcaatcgg cgaactttat tgaaaatagg
    49381 cggtagcgtg atgggacttt ctttcatcct accagctgtt ttaggaactg tactggatgc
    49441 tcatacaaat tcgttgttga ttctcttgtt tttatgtatt tatgtcgcat tttattcttg
    49501 cacatgggct ccactgacat gggtaatcgt aggcgaaata ttcccactag ctgtccgcgg
    49561 gcgagcatct ggcctagcct cttcttttaa ttggattggt tctttcctag ttggcttgct
    49621 ttttcctgtg atgactgctt ctatgtctca agaaatcgtt ttcggcattt ttgggatcat
    49681 ctgcttttta ggggttttat ttattcaaga gatcgtacca gaaactcgtg ggaaatcttt
    49741 agaggaaatc gaacaaagtg catctaaaaa aacttatcct aaacgtattt ctatccatca
    49801 ttcttaaaga ggttgtgaca gaagtggtca gcttcaagaa ataagaagga accgcttagt
    49861 ttccagtcac aaggaacaat ttcaaaaaac agcttctcat gtttgatgaa tattgtgact
    49921 tgtgaccagt tggtaggaga acaccgttta gtggagaggt ggtgaaagaa acgttcagcc
    49981 ttgagcgatg acaaaagctc tcacaaacat tgcgacgagt aaccgtagga gcaataagag
    50041 gcggtaaaaa cccgtctctt atttttcgtt ttctttcact gtttcccgtt caactagtac
    50101 aggttcatag agaatatttg gctgtgcctg tcctttttca atcgttgtga cgatccaccg
    50161 tgcggcatct tgtcccaact tttcttttgg atgcgtcaat gttgtcaatt ttttattccc
    50221 taaatggctt aaagaagaat cgtctgttcc tacgatcgat aggtcatctg gaatatgata
    50281 gccattttct gtcaacttat ctagcattaa actagctatt tgatcgttat agcaaatgat
    50341 gccatctaaa gcttgttctt ctttcaaccg ttgtactgtt ttttcagcga ttgtttctcg
    50401 tgtttccgtg gtgaaagtaa tgatatcttc cgtcaaaaaa cgcacattat actgttcaca
    50461 cgcgctgata aaacctttca tcctgtattt tccttgaaga tcgtcgattt ttgtgatgaa
    50521 caacagacgc tgatgtcctt tttttatcaa atattctgct gccataaaac cgactttcac
    50581 atcatctaaa caaatataag aggcatttaa ctcttcataa gcagcattga tcatcaccat
    50641 cggaatgcct tgttctctca aagcaacata aagtgctaag tttgggttat actgattact
    50701 tttcgtaggc tcaacgatca aaccatctac cccataagca atcattttct tcaaacactc
    50761 tttctccaat tcgtgatcgt tattcgtact ggctaaaagc aatgaatacc cagattctcg
    50821 taattcattt tcgattcctc ggataatcga cggaaaaata taatcagaga gatatgtggt
    50881 gatgacaccg atcgtttttg ttctgttcaa tggtgttttt tctttcttat agctgtcatc
    50941 tacatatgtg cccgaccctt tttctttacg cagatagccc tcatttacca acacagcgat
    51001 tgcttgccga atcgtatgtc tgctgacgtc atatatttct tgcagcttga attctgaggg
    51061 aatggttgta tttacttcgt attcctcacg aaggatcttt tgttttatct cttctgcaat
    51121 atcttgatat tttgccattt gccttgcccc tttttatttg tacgtacata tatcataacg
    51181 agtctgcaag gtttattcaa gcaaaaatca aggtttcttg tgctcataag gcatacggaa
    51241 caggttcacc aaattctgga aatccttgct cattccacgt aaagcattgc gcgttcgcgt
    51301 gccgattagg attgtctaac ggatcccctt tttggttctt ctctggtcga gcatgataga
    51361 tcaaaatatc aaagcacccg tcttctgaaa ccgtaaacga gttgtgacca ggaccaaatt
    51421 gcttattttt tttagaagaa acgaatactg gctccgataa tttatgccat gattctttta
    51481 ataaaagatt cttgtgctca tcagcccaca gcaggcccat ggcatagttt tcatctgttg
    51541 cactaccaga atacgtaatg aatattttcc cattgcgagt aaggactgct ggaccttcat
    51601 ttactgaaaa accccgtttt tcccattcat actcaggtat agaaaggagg atctgcttcc
    51661 cctttaacgt ccaaggatta tccatttcgg atagataaag attagaattc ccagtaattt
    51721 caggatcttg ttgggcccac acataatata gctttccttg agaagagaaa gttgttccgt
    51781 ctaaactgaa agtttccctt ttcgttttta tttgtccctt ttctatccaa tctgtattca
    51841 ttggatctat atgctgattt tcaagtacga acatgcggtg atgatgagat tcatcacgta
    51901 tagttttatg attgcttgca gcaaagtaaa tataccattt tccattgata taatggactt
    51961 caggtgccca gatcaattca ctcatcggtc cttcttcatg agcctgccat acaatcaggc
    52021 ttgcagcatg ttctaattca ttcagtgact ttgcccttct taattcaatc acttggtagc
    52081 ctggaaccga tcctgtaaaa taataataac catctgtatg tttatatacc caaggatctg
    52141 cccgttgccg aacgatagga ttattataaa gtgtcttcat cataacaaat ttcctcacat
    52201 tttctttatt ctaattacat tccaagagta tttttctatc cctacattta attcgtcatg
    52261 tttttctatt gaaaaatagt ttatttcagg ttttattttt tctggttctt cacgtgtatt
    52321 tttgtccaac agatcgctac tggttaatgt gtagtgctct ccttgatcta gcaaattgaa
    52381 atcatgttca atcgttatct tttgttgttc atttgtgcga ttgatcaaga aaatagtcag
    52441 ctgattgccg ttatctacga tcacactgtc cacataagga acatttttta tcttttcaca
    52501 atcataaaca gggcaaatga tttgttgttt caatacttga cctttagcat atttcgataa
    52561 gtgttgcatc acataataag ttggattcct ccatgcttgt cctttagcat ctgtcagtat
    52621 taaaggtatt gtgtttacta acaatgattg acaactaatc tttacgcgat ctgcgtgtcg
    52681 taaaatagcc agccctaatg aaccaaataa taatgtatct tccatagtaa agtaacacca
    52741 atcgatggga ctaccgactt caaaatccct atgtcttttt tccggttggg catgtacatt
    52801 ccattcatca aacgctagat acattgtttt ctcacttctc aaatatcctt ttacataatc
    52861 acaagtagca acgatttctt caatttgacg atcgaatcga taagcttttg caaggtatgt
    52921 cgctgtatca tcacgctcat ttttaggagg tttagtcaag tcttcttcat catacttatc
    52981 aatatagtta tgcaaagcta aataatctac attctcataa gcttcttcta atgcgatacg
    53041 atcccattca gggtacgatg gtaaacgtgg cgtagagctt ccaactaaga ttgtttcaat
    53101 tgaatcatcc actaacttca tgacttttga ggcttcattt gctaacctac catattcata
    53161 agcactctta gcccccactt gccatggacc gtctagctca ttccctaaac accataattt
    53221 aacgccaaaa ggcttttttt gcccattttt tattcttaaa tcactccaga aggttccttt
    53281 tggaaaatta caatattcta ctaaattttt tgcttcatca attccttttg ttcctaaatt
    53341 caccgtgaat atcggtttcg cttgtacatg actgatccat ttaaaaaatt cgtggagtcc
    53401 aaaagaattt ggttctatac ctcgccatgc taaatcaatt ttttttggtc gttgttcttt
    53461 cctacctaca gtatccaccc aatcataacc agatgtaaag tttcctccag gatagcgaat
    53521 gacacctaat tctaattcct ttacataaga gaggacatct tgtctaaacc cgtcaccatc
    53581 agctgtttca tggcccggtt cataaatccc tgtatagacg acacttccca tatgctcaac
    53641 aaatgagcca aacagccgag aactgatctc tcctaccgta aaatcctctc tgatttttac
    53701 tttcacttct tcgtccaaca gctttcccta cctttcattt cctatagtca agataataga
    53761 tacaagtttc catacgccac cagcaaagat aaaaataaaa ggagggaaaa gaaatatgag
    53821 cagtacaaat cctaataaca atacgcaaca aagaatattt ctcaaaaaat caaaaaatac
    53881 tttccatgca ctttgttcaa aaatttgtaa aaatgtgata tctttgtttt cagaaatcac
    53941 atatatccag gataaaagaa acatggaata aataagtaaa actacatatc cactaattga
    54001 aagtacgatt gtctgttctt ctaataattg attaacaaga aaataaataa tgatcgttaa
    54061 cagactaagg aacaagttct taccaaaatg ttgctttatt ccttgaaaga aaaaataaat
    54121 gatatttcct gatccctcac ctttccattg cgccattaca taaaaaacag cacatgtagc
    54181 aggcacgata gtaataacag gcaaacacac gattacccag agaataccta ataagatgta
    54241 atcagaaacc acaatcaact gtccataaag tttagtctca actattttca tcttcctcac
    54301 tctttcattc cagacatagc tgttccttga acaaaatatt tctgtgcaaa aatataaacc
    54361 actagtaatg gcagcattga aataaccgtt ccagccatta caggtccata aaaagaaatg
    54421 ttctgccctg taaataaagc taaccctgct ggtaaagttc tcatttttga actacttgtc
    54481 aatatcaatg gataaagcaa gtcattccaa ctattcatca gcgtgaaaat ccctaatgat
    54541 aaaagagtag gtttgctcaa tggcaacatg attttcaaaa aaatattaaa ctcacttaat
    54601 ccatcaatgc gtgctgcctc ttccaaagaa tctggtaaag taataaaaaa ggaacgcatc
    54661 atgaagatac caaatgcagt tgtcatacga ggaatgatca aacctgcata agtatcaagc
    54721 atgccaagca aattgacttc aataaataga ggaatcatga agacttggaa tgggatcatc
    54781 attgtcagca aaacgaaata gaacaaaact gattttcctt tgaaacgcat acgagcaaat
    54841 gcataccccg caagagaatc aaagaataca gaagtgatca ctgtccctcc agcaaaaatc
    54901 actgtatttt tgatgtaatc taataaaggg atcgttgtcc aaaccttaat atagttatct
    54961 agggtatagc tatctgcaaa tattctaggc ggaaatgaga taatgtcttt ttcgtgttta
    55021 aaagaagaaa taatcagcca caaaaaagga aacgcaataa ccaacaatat aactagcata
    55081 ctaatcgttc gaatgatttt tttaccacta actgcttttc gactattcac agtaactgta
    55141 atcgagcttt ctggtgccat acaggtttcc ctcctatttc atttgttttt ctttccgtga
    55201 catgtacaca ttagtggaga tagctatagt tgcaacaatg ataaacaaaa ccgaagacag
    55261 tgctgatgca tagcccaatt cataaggtgc tgtaaatcct cgactgtaaa tatattgaac
    55321 agctgtttct gtcttaaatt gtgggccacc agcggttgca acatatactt ggtcaaacac
    55381 ttgcatcgaa ccaatcagat tcgttaaaat acaaaaacct aaggagggaa cgatctgagg
    55441 tagcgtaatg ttaaaaaact gctgtcgatt tgttgcacca tccatttgag cagattcata
    55501 caaagaggga gagattccct gaatagcagt caagagaatc gtcatagtaa caccaaaatt
    55561 cttccaaaca gtcatgatgg ctaccgtcgg catagcaagg gttggatcgc gaaaaaattg
    55621 agggatcggc aagccgactt gtgaaaacaa gtaaggaatc gcccctacat tcggatcaag
    55681 caacatagaa aaaataatcg caattgacgt caatgaacaa ataactggaa tgtaaaatgt
    55741 cgaacgacaa aaacgattga atctggtatt tttagataat gctactgctg ctaataaacc
    55801 aacgataatt tgtgttggcg tttcgagtac ggtgaaatat aaagaatgaa tcaacgaatt
    55861 aactgctcgt tcatcactaa atatacgtgt gaaattttca aaacttacaa aatttcttga
    55921 tgtaaagaaa atattcaaat ctgtaaaact aatagcaaat gttcctaaaa gcggtatgac
    55981 agtaaataat aataaaacta aaaaagatgg agttaaaaac aaatacgcag cacgatcagg
    56041 acgattccaa tatttggtga atccgttcat atagtccacc tcatctttca ttttgataaa
    56101 atatacaatc ttggtttaaa cgaatctgaa taagatatcc gaacaatcgc tgtgattttt
    56161 gcaataacac ctatctgcaa atactaggtc gatcattcag atatctgtat cttgccaaca
    56221 gtctatcctg tataaagttc tgtactattt ttgcagtaat tgattgatgg catcatctgc
    56281 atcgttcatg agtgtctcag gatcttctcc tgctaataat ttttcaatca gtggattgat
    56341 gatatcgtta ttgatcgcag agatttgggt aaaacctggt aaatatggct ctgcatagtc
    56401 catttgtttc gataattcag atacaatagg atcatttttt acttcttcat cttttgctac
    56461 tgaatccaag taggcaggaa aaccattttc gatactccat tttttaccaa tttcagttgt
    56521 attccaatat ttcaaaaact catagacagc ttctttttta ttattatctg tacttgccgg
    56581 aatggcatat cctacgccat ctaaaatagc tgtttttttg tctgaatcac tcccttgtgg
    56641 taaggtagtc acattataat caatctcgtt tgctttcaat ccgttattta accatggccc
    56701 attgatttca atggctagtt gaccagcatt cattaaattg tccatttcgg cgccagaaat
    56761 cgattctgga ccagtcttgt ctacatggat cattttttgg atcgctttta acgttttaag
    56821 gttctcagga gacgcaaatt cagacttagt ataatcatcg ttcactaatt taccgccatt
    56881 tgctaacatc cagttatata acggtgcagt tccatcctta ttgaagacaa aacctgatac
    56941 atttttacta ctatcagtta attttggtgc catttctgtc aattcttccc aagtagtagg
    57001 tggttgttca gtatctaatc cagcttgttt aaacaaatca ttattccaat acatatacat
    57061 gccttgtacc tgcatgggga caaaatactg tttaccttct acttcaccta attcaatagc
    57121 cgtttcttta aaatcagatt tatctactcc atcatattcc cagaaatcgt ctaatggttg
    57181 taccgctccg ttttctacat actgtgcaaa atctggataa tttaatgcaa taaagtcagg
    57241 tgctttttta gaagaaatcg ctgtaggaag tttttcattc aaattggccc aagtcatgat
    57301 atccatttca attttgatgt ttttatcatt tgttttatta aattcattaa cgatttcttg
    57361 caatatttca ccatcagatg cagtaaaccc attccaaaaa gtcaaagtca cttctttatc
    57421 agaatcagat tctgctgaac ttccgccacc gcaagctcct aaaatacctg ttgcagctac
    57481 caacaaagag ccaattaacc attttttacc tttcattttt tgttcctcct tcatctgata
    57541 aacgacatct gaccacattc caagatagcg gtttcaataa acaattgatt ttcccatctt
    57601 cagcttttac atttgttgaa tgacttggtt ttacttgttc acttttagga agattagttg
    57661 cttttgtatc aaatccactc atttcagaaa aatcaatcac tccctctaat gataattcag
    57721 acaagtgaac agttagattc atttcttctt ctccacggtt aactgcaaat atgacaagtt
    57781 ctttgtcatt tattacggct atactttcta aatacggcac ttcttcaaaa tcttccgtcg
    57841 catagtttgc tgaacgaatt tgtgggctta acacctctcc ttgtccatac acagcagctt
    57901 gcataaatgg ataatagatc gtctgtctcc aagcttctcc gtttgtttct gtcctgattg
    57961 gtgcaataac atttaccaat tgagcaagac aagcaatctt tactctatct gcatgtttca
    58021 ataaagtgat taatagacag cctaatagta atgcatcttc aaaattatag ttatcttcta
    58081 gtaaaggagg agcaatctgc caaggcgctg cttgctcatc ttgttccgct gaatgatacc
    58141 aaatattcca ttcatcaaag gacaggttga tctgcttctt actatgtttc ttcgccttga
    58201 ctgtatcgca catcgctgtc acttcagaaa tgaaccgatc catgtctaag gaacgtgcaa
    58261 gataatgaga aagatcattt ttttggttgc cataatattg atgcagagac aaataatcta
    58321 tttgatcgta cgcttgatca agaactgtta actcccaatc accaaatgta ggcatttgcc
    58381 gattcgaact tccgcataaa accacctcaa ttgaatcatc tacgagtttc atcgccttag
    58441 ccgtttcctc tgctaaccgt ccatattctt ccgctgtttt atgtccaatc tgccaaggac
    58501 cgtccatttc attccctaaa caccatgtct taattccaaa agggctctct tgaccattcg
    58561 aacgccttaa atcactccaa taagttcctt taggaaaatt acaatattcg actaaattac
    58621 gagctgcatc tactccacga gtacccagat tgacagccat attgatttcg gcccccacat
    58681 gctgacacca gttataaaat tcatgaatcc caacttcatt cgtttcaatt gtccgccaag
    58741 ctaaatccaa ccttctaggt ctatttgcta tgggcccaat actgtcttcc caacgaaatc
    58801 ctgacacaaa atttccacca ggatatctaa tcatgggaat gttcaattct tttaccaagt
    58861 gaatgacgtc ttggcgaaat ccacttttat tagctgatgg atgatctggc tgataaatcc
    58921 cttcatatac tgctcgtccc atatgttcaa taaatgaact gtacaaccgc ggatcaactt
    58981 tgcctacacg cttgacacta ctaatcaaca ttgtggcttc cataactgac ctcctttaat
    59041 ttatacatga ataatacacc tcgttgaaag cgttgtcaat aatataaatg ttaaacttac
    59101 ttatttttta tcgattttaa ttaaccaaaa tattaataat gattaaaaca aataatttaa
    59161 tataactttt attaacaaat atgttaatat ataagaaaaa ccttttaaga ggtgatatat
    59221 atgcaactac aaataaacga agaatcctta ccggtatatg aagcattagc tagtaaaaca
    59281 aggatcaaga tcatccagct actttccaaa aaaaagatga atgtcaaaga tttggcaaaa
    59341 gaacttggag tgagcagtgc tatcacgacg atgcacgtga aaaagctaga ggaagcaaac
    59401 atcatcaaaa cagaaaaagt aggacaacaa aaaatttcga gccttcgtgt cgataaaatc
    59461 gatatcagtt ttccagaaaa aatattcaat gcttttgata cgaaagaaac atcgatacct
    59521 attggtcact atacaaatta tgcaattgaa ccaacatgcg gtttagctac gatccatgat
    59581 tttatcggga aagtggatga accacgctat tttatggatc ctcgaaggat ggatgcccgc
    59641 attctctggt ttaccagcgg atttgtcgag tatcaggcac ctaatttttt aaatacagag
    59701 gatacattgg agatgttgga agtttctgtg gaaatttctt ctgaatttcc tttttcaaat
    59761 gacaattggc cttcagatat cactttcagc ctaaatggtg tggagctagg aacatggacg
    59821 agccctggag attttgctga tatccgcggt aaatatacgc ctgattggta tccagataat
    59881 cttaatcaat acggcttgct gaagacgatc cgaattacca aacacttgac gaatatgaat
    59941 ggagaaccct tgtcaaacat cacgatcaac gacatcccta aagagcaaga cacttggcat
    60001 ttgcgaatcg aagtgaaaga cgatgccaaa catgtggggg gctgtacttt atttggtaaa
    60061 ggattcggta attacgatca ggatatcaaa ttaaaagttt actacagctg atatttttct
    60121 tttctatttt ataaaacaaa aacagccaat ccctatgaag ggacggctgt ttttgcttta
    60181 ttgttatttc cagaaatcat ccaaaacgga aattggcaaa tgtcgtttat gttgtgtctt
    60241 gttccaccat gcttcgattt tttcttgtgc ttgagcggga acttcttttc cttctagata
    60301 atcatcaatt tcattatatg tgacacctag tgcgacttca tctgcaatca ttggtttccc
    60361 atcttcaaga tctgcagtcg gaatttttgt gtataatttt tccggtgcat tcaatgcttg
    60421 aagcaattgt ttcccttgtc gtttgtccaa acggaataat ggcaaaatgt cggctccacc
    60481 atcaccaaat ttcgtgaaga atccagtaat gttttctgca gcatgatctg tccctaatac
    60541 ggctcctgct ctttctccag ctacggcata ttgcgtaatc atacgttgac gtgctttgat
    60601 atttcctttg ttaaaatcgc tgattcccac acctgcttcg cttaatactt gtacttgtgc
    60661 atcaacagct ggtttgatat ttactcgcag actaacatcg ggctggataa agtcaagagc
    60721 agcttttgca tcttcttcat ctgcttgttc accataagga agacgaacag cgataaattg
    60781 atagttttca tcttttgttt cttcacgcat ttcttccatc gttagttgcg ccaaacggcc
    60841 tgctaaagta gaatcttggc cgccgctgat acctagtaca aaagttttaa gaaacggata
    60901 ttttttcaaa tatgctttca taaagtcaat acttttacgt atttcttctt ttggatcaat
    60961 tgttggtttg acacctaatt cattgataat ttctgtctgc aggctcattt attctcctcc
    61021 tatgcttctg tatgaagttc tgctacgctt tttttcattc gttccatgat agccatttta
    61081 tggttccagc attcagtaga cagatcgact gggtattttt gcggattcaa gtctcgttta
    61141 tactcttccc atagtgaatc tagattttct tttgtatatt ctttgatttc gtttagtgca
    61201 ggtaattcgt aaactctttt tccttcgata aagatatctt gtaaaactgg gcgagctgta
    61261 aagtctcgaa cagttttatt gataaacgta tgcactggat ggaacatgaa gatagcttct
    61321 tcttctcgag ggtcttcatc ccataatgtc acgtagtctc cttcagattt cccatcaaaa
    61381 tttcgagtga tgcgccacac ttgttttttc cctggagtag ttactttttc tgcattgctt
    61441 gataacttga tcgtatccat catttttcct tcatcatctt cgatcgatac tagcttgaat
    61501 actgcaccaa gggctggctg atcataggct gtgatcaatt ttgtcccaac accccataca
    61561 tcaattttag ctttttgcat ctttaaattc aaaatcgttg cttcatccaa gtcattcgaa
    61621 gcatagattt ttgcttccgt aaatccggct gcatctaatt gttcccgaac acgcttagat
    61681 atataggcca tatctccact gtcgatgcgt acgccttgga agttgatttt gtcacctaat
    61741 tctttcgcca cgcgaatcgc acttggcaca cctgatttca acgtatcata agtgtcgact
    61801 aagaacacgc agtctttatg ggttttagcg taggccataa atgcttcata atcatttcca
    61861 taagattgga ctaacgaatg agcatgggtc ccgctagctg gtatcccaaa tatcttccca
    61921 gcacgtacat tgcttgttgc atctgctccg ccgatatatg ccgctctagt tccccagact
    61981 gctgcatcca gttcttgtgc ccgtctagtt ccaaattcca atagtggatc ttctccgatg
    62041 acagatttga tacgagctgc ttttgtcgcg atcaacgttt ggaaattgac catattcaac
    62101 agtgctgttt ctactaattg gcagtgcgct aaaggccctt ccacttggat taatggttca
    62161 tttgcaaaaa ctagttctcc ttcacgagca gaacgaactg ttgctttaaa ttcaaaattt
    62221 tgtaaatact ccaaaaaacc ttcaggatag acttctaatc cacgcaagta agcaatatcg
    62281 gaatcattga acgtcagatt ctctaaataa ttgactaacc gttccaatcc tgcaaaaacc
    62341 gcgtatccgt ggttaaaagg catttctcga aagtaacatt caaaaacagc atgtaaatca
    62401 gctcgaccta attcccagta tgtctgcatc atattgattt gatatagatc cgtatgcagc
    62461 gtcaaactgt cgtctgggta agtattattc atcttgtatt actcctcttg gcaagttttc
    62521 atttcctacc cattataaca aaaaaaatta ataggaaaag aaatcttcca ttttttctga
    62581 aaaagcacat gttttacgga tatttcttgc taaaaatgaa ctccatctgc taaaactatc
    62641 ctttcttatt ggtatttaac ctaaacgaga aagaatactt attttattca atgtatccga
    62701 gacacgaaga aattagcgtt ttctattttt cttacttgat aaaataagta gattttccta
    62761 ctacaatcgt agccgttaag ataccgatga ttgaacaaat ataaccaaat gtctctacaa
    62821 cataatcaat ggcaaaagtt tttgaagcaa taatacgtct ttcctttttt gcttcttatc
    62881 cccaaagaaa cggatataac ttattatgta ttgaattttt ttggaaaggc gaattatgac
    62941 aagatatacc ttgcgttgaa aaaactcgcg gaaaaaatat cacacgtttt taaaaagata
    63001 caaagtaaga aagcagctgt caatagaatg gaaacaaaaa tggcgagaca tcaatcagtc
    63061 tcgccttgtt ttttattgta tacattagat atggagcagg ttaccataaa gatgattatt
    63121 agtcgttatt aaatagaatc cttcgtattt ttttgttcta tcactacaat atctttattt
    63181 ggacgtttga attgtattcc ggcacgttct agctcctgtt tcactcctac atggactttg
    63241 acatcttctc gtgtcatttt taaaacgtga tgaatcgcat agtcgatttc ttgccttgtg
    63301 atttttttct tcgtattgat catcacgact tgctttttat catatgtgtc taccaatagc
    63361 agttgtgttc gatctaacga aaataatttg taataattaa tagccgtatt cccaaaaaag
    63421 aattccacta ctttaggata aaatgtctta atatttaaat tactattcac aggtgtttcg
    63481 attagcttca tctcattcct cttttctata tagtcgattt agatccaatc ttaagttatg
    63541 ttaccatctt tcctagcatt aaggaatcgg ttacaattga acctttaaag acaaaaggat
    63601 ttacctttta tcacttaata gtataacata atttcgtgag aaaaaaatac aattttttct
    63661 tgaccatctc gttacgtaaa ggattatgct tacatttgtc aggaggaaaa ataatggagt
    63721 acacaataaa gaaaatgtca gagatatcag gagtcagtcc gagaaccttg cgcttttatg
    63781 atgagattgg actgctaaaa ccagctagga tcaattcttc aggttatcgg atttatggca
    63841 aaaaagaagt cgatcgtctg caacacatct tattttatcg aacaatggca ttcaagttgg
    63901 atgatatcca agaggtctta gacaatccat cttttgacca ccagaaagct ttaatcaagc
    63961 atcagcaaat gttgctggaa aaacgggcac aaatcgatac cctcttgaca actgtccaac
    64021 agacacttga tatgtacgaa ggaggaagaa aaatgagtga caaagaaaaa tttgaagggt
    64081 ttaaacaaca aaagttgaaa gaaaatgaag aatcctatgg acaagagatt cgacaaaaat
    64141 acggaaaaaa aaaggttaca gcatccaatg aaaaatggat gaatatggat aaagaaacgt
    64201 atgaagaaat gcaaactgtt gaaaagcaga tgttatttga tctcactctt tacttaaaag
    64261 aaccagaaga tgaaagctta gcagaccgta tcttccaagc tcataagaag tggttctctt
    64321 acagctggcc tgaatatcag ccaaaagcgc ataaaggact aggaatgatg tacgaagccg
    64381 atgaacggtt cactgcatat tatgacgagc gaagtggtca tggtgcagca aaagctttaa
    64441 atgaaattat ccaaaaaaag gcgtaatctt ttacatgatc caacaaaaaa gtacacgtaa
    64501 aatgacctaa taacaaaaca gcagacaccc aagttagatt tttatgctaa cttttggtgt
    64561 ctgctccatt ctttcacaag ttttttcatc ctactttaaa tatattcatc gttgaataaa
    64621 cgtataaggt attttcactt tatccttttt ttctgtaaga aacaattgaa atgccatttc
    64681 tccgcaagct tccagatggt ggtcgatggt tgggaaacca agaagctcgc tgatcagcaa
    64741 attatcgcgt ccaatcacca ctggtgtctt atcttgcaga tagctttgca agatgcctgc
    64801 tgcaaccata tccgaatttg taaagacacc atcaactcca agctctttaa aaaagaagcc
    64861 tgcttgtttt cctttctctg catctataca atcccaaaaa acaagtgatt catcaaaaga
    64921 tggaaaaagt tgatgacata gctgcaaagt gatttttgag ttataactta gcctgccact
    64981 tcgccccaac gtgatgccca attgctttat accttgctgc ttcaaataag taagaccttc
    65041 tgtaattgat tgttctcgat caataaagac acagcttgcc tgttttccct tgatttcttc
    65101 acaaaaaact agaggtccat atttttgata tgccaataaa tcatctagtg tattggcacg
    65161 agacgtaatg atcaatccat cgaaagcttt agctgcaaat tcttcaagat accttcgctc
    65221 tttttcttta tgatagcccg tcggtaaaag tgtcactttg taatcataat caaacgctgc
    65281 ttcaatgatc ccactgatca actgatcaaa atacggatga ttggtataag gcagaatcac
    65341 tccaatgctt ttcgtttttc cataacttaa ctgtctggca tttgcattag ggatataatc
    65401 gacttctttc atgaccgcaa gtatttcttt ccttttttcc tcatctacat atggatgtcc
    65461 attgattaca cgtgaaatag tagaaactga ataacctgtg agctttgcga tatctcgaat
    65521 gtttgccact gctgcttcgc cacctataca aaaagatttt cttgtaaata gtctaccaca
    65581 gctggaccgc attcaaaaaa ctgatacatt tttccttcat gcgaaaaact gatccgctgc
    65641 ccgccccaat atatatcttt tgtcaaattt tcgatttcat tcattggtat ttcttttact
    65701 atattcctat tcaacccttg aaaactgtac aacgttaata cggatccctt gaactcgaca
    65761 aacgcataaa aataattgtt cgttgtgcca tctgttatct ctacataagt attcattgca
    65821 agccctcctt ttgatacttt gagtatagtg tatgaaacgc gtctcatagc aaggagtttg
    65881 ttttgccaaa gaaaagtttt attcctcttt cattattacc aaagtctctt attttaaatt
    65941 gaatacatta taatgatact gtaaatatat ctttattatt agtgaatgga gtcaagtcca
    66001 caatcttgtg taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac
    66061 tgccatgcct ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt
    66121 ctctcctcag aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa
    66181 ttctaaatat tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc
    66241 aattatccga gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg
    66301 aatctcttcg ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc
    66361 aggaaaagac aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc
    66421 acttaatttt gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa
    66481 ttcaagttca gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt
    66541 ctttggcagc ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc
    66601 acctaaaaag ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag
    66661 ttttacaccg aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc
    66721 cgattcgccg tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc
    66781 gataccaata tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag
    66841 ggcatcacac atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa
    66901 aacaaatgga tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc
    66961 atccgttagc agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat
    67021 ttcaatcatg gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg
    67081 actaaactta ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt
    67141 aaacgaacga tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc
    67201 atttgcctga atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat
    67261 aagtgttttt gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct
    67321 ttcaaaagta aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca
    67381 aaaaacattg tactcctaac tgagatcaat ggcttttttg atgcttcact tttacacaat
    67441 tatatggacg taatcaatta atatcatttt ttaacatagt aatttgttta tttaaatctg
    67501 atagtgtttc ttccttactt aatataaatc tttctctatc atgtaattta tttagttttt
    67561 catcattttt ttgttctaat tcataagctt ctttttttat tgtgttagct ttcgacagtt
    67621 tagtgattcc tataattaat aaaataagtc caatcaataa taacattcaa aactcctcat
    67681 ttctttgata taattattgt agagtttact cagaaatgag gaaagagtcc atgtgaaagc
    67741 atgggctttt ttatttttaa tgttgtaata ctaaatgttc aagattatga ggaagcccta
    67801 aatattcaat aatttggaac tgactcatat cagataaatc ttgttcatct attgttctag
    67861 ttaacatttc aattgcaaac atattagctt cagcttctat accataaatc atacaactaa
    67921 ggttgtttgc tctaaaagct gctgtgcaaa tacctttatg gagtctaacg tgggctattt
    67981 catgccataa tacaaatctt ttcatagaag gtgataggtt ttgattaatt aatatcacat
    68041 aacatctttt attgtgtatc gtttgtccta atgttttatt ccctaaatca gtttcaatta
    68101 aatctatatc agaatatttg attattttat atggattaat cgcatcagat ccaaactcta
    68161 caaataattt ttctatttct tctaatatcc agtatttgtt taaatgcata ccaaagccca
    68221 cctattgacg atatttttta ggagtgaatt ttttctttgc tttaactttg gttaaggcaa
    68281 gtgcaagttg aatggcatcg tataattttt ccttatcttc atcgtctaaa ggttcaccat
    68341 aataatttgt ttcagcatct gaattcaagc cttcaagtaa tttttcagct tgtaaagcaa
    68401 tatcttgttt atctttttct gttaaatcat aataatggcg tttatcagta cgtcctaaaa
    68461 gataatcagt tgatacatta aagtaatctg caactttttg tattttatct ccagatggag
    68521 tacttttatt ccatctagac atgcttccat ttccgaaatt taactttctt tcaagttctg
    68581 ccaacgtcat tgaacgttct tttaataaga tttttatacg gtctaatagc ataggatatg
    68641 cattcctttc aaaataccga caaaaaaagt atttttctgc aaattatcta tttacaaata
    68701 gagaatttgc agttatacta gttttgtaag caagatattt acggcataca aagactaaaa
    68761 gcaataaact aatagtctcc ccagacatca gtaagctcgc tttttgtgcg ctgtattttg
    68821 tatgcttaca taatagcata tttgcagttt ttgtcaaact tattagacaa aaatattaga
    68881 aaatattcga taagggggta tgaagcatga ctttaaaaca gcaagtatta attgctttag
    68941 taaagaaagg ttggagtcaa agagagttag ctcgacgaat gggtatatca attacttatt
    69001 tacgagatat cttcatcgaa aaaagaaaac ctaaggaacg tttaaaacaa atcgaagaac
    69061 ttttggatat caagttagaa gtagattcta atgatcagcc atcagaacag gaggcgtaaa
    69121 aatggtacaa cgtttgagca aagcggagtt taaagagaaa tataagtatt ctgaaagtac
    69181 ttatcaaaga agaatgaagg aatttaaaaa atcacgtttt agtgaggggt ataacgcagt
    69241 tacttccaaa gaaataacaa ttgatgtcga tttatatgaa cagtttagaa catggcgttc
    69301 taaaaatcga tttagagtta gagctgtttg ttaatagcta aaaaatctaa attcaccact
    69361 tctgaaaaga ggagttgctg ctctcctttc aagtttgcca aagtccacgt tttttcattt
    69421 tggcaactcc tcttcttgga ggtggtgacg gcgagagacg aaggagggat gacatgaagg
    69481 aagaatcaaa aatagggatt ttgaatgaga acaattacac aagattaatt attgaaacag
    69541 aagaagggaa aaaagtagca gaagttacat tgaccgatgc aactcctgct gacggttatc
    69601 gtattcgatt aactcctaaa tatgattaac cttttggtgg gtgtggatcg tttccgtgac
    69661 tatcacggct ttgaattttt ccatctctgc catgaataaa tagctcagat ccttgatttt
    69721 tagaaatctc tctagcgatt tttactgctt ctgattttgt attagtatgt gcagtcgctt
    69781 tagaatttcc agcacctttg acattccaac caccattgga attaggtaca acatgttgat
    69841 tttttcccat agttttcacc tccatttcta tagaaatatt ttatcaaata gaaatggaga
    69901 aattcaataa acgaaaggag aagtgatatg gcatatacct tgcaacaaga acatcaaatt
    69961 ctcggtttga ttaaacaacg caggaaacaa ttacaagatg accgtgcagc gcttagaaaa
    70021 gccgatgagc tatcagatag gcaagctgaa ctaattgctt ctgaacttga ggatttgaga
    70081 atgctagaaa taaaaaatag ggagattaga ttatgaagaa gacagacaca ctttttatag
    70141 gattcatttt ggggttatta gtgattgtag cgcaccaaag tattatcggg ggaagcttgt
    70201 tcgcagcatt gatggtttta atcaatctgc ttgattcaaa agaaaggagc aactatggca
    70261 cgagaagaag cgctaaaaat cggtaaagtg attgctgata atcggtgggg aaatagccgc
    70321 cctattattt taagcaagca acatatcgaa aagcaaaaag catggcaaca aataaaaaag
    70381 tgactctgcc agccagcaaa gagtcacata caaatttata aaactaggag aattatatca
    70441 catgaataag aaaattgaaa atcttatcga agaattgaaa cgtgaatgcc aaaagcaagg
    70501 tgtttctatt atttgtacag cccaaaaaga aggggaactt aaaagtctcg tttatggtga
    70561 gacaacagaa attttacttt gtcttgctat gcaagaagaa catttagatg aaaatctccc
    70621 attgtcagca catattatgc gtagaattgc tgtggatgct tacgaacaag cgaaaaatga
    70681 agaagaaaat caaccttcaa atcatacttt tgttattaat aacaaggaag atttagctga
    70741 tgtgatgact cgtattctca agggggaatt tcaatgatga atgaaaaaac aattgatcta
    70801 ctcattattt tatttttcac gttagtgatt gtagcgaaaa gttatgtaat ctttggcttg
    70861 ttattcgcaa ttaccatgct tgtgctttgc gtgttagcaa gcagaaaaga ggttaaacat
    70921 gaacgagtta gcaaatcttg acaactattt aactgatcct gattatgcta aaccaccata
    70981 tgaagcacca attgatgagg aggatgaaga tgagtaaatc taccttagaa atgagccatc
    71041 aagaatggct tgaagaccgt aagaaaggca tcggaggttc ggatgttgga acaattttag
    71101 gattgaataa atggaaatct ccttatcaac tatggctaga aaaaacagga caagtagtac
    71161 ttgaagaatc aggaagtgag cccgcttatt ggggtaatgt tttagaagaa gtggttgcta
    71221 aagagtttca ggaacgtaca ggcaaaaaag tacgcagaag aaaccaagta tttgaacatc
    71281 cattacatcc atttttaaga gcaaatattg atcgggaagt agtgggagaa aatgccattc
    71341 tggaatgcaa aacagctaat caatttctcg gtaaagaatg ggaaggcgaa gaggtaccgc
    71401 ttagctatct ctgtcaagtt caacattaca tgaacgttct aaacaaagac tattgttaca
    71461 tcgctgtctt aatcggtggt caaaaattta tctggaagcg gattgaacga gatcaagagc
    71521 tgatcgatac aatcactgaa caattagtag agttttggga aacaaacgtt cttggaggta
    71581 tcgagccaat tattgatggt agtcaagcga cagctgattt cttgaaagag aagtatgcag
    71641 atgttgaaga cgttcaaacg acgttaccaa ttcatttcga tgaactagtt gaacagaaaa
    71701 acgaactcaa acggactaag aaagaaattg aatcagctat ccgtcaagta gacaacgaga
    71761 ttatcagcga gctaggtaaa cgtgaagcca gtatcggtat cactcaaaaa aacatcatca
    71821 gttggaaatt tgtccgtacg agacgtatga actcgaagaa actagcagag aaatatccag
    71881 atgtcgcaaa tgatgaagag atttataacg ttactgaatc aagaaggcta accgaaaggg
    71941 agatcaaata atatggcaac aaatgaatcg ttaaaaaatc aattggcagc aaagccacag
    72001 aaacaagttg caccaggtca attaggactt aaagctttga tgaatacacc aacaatgaga
    72061 aagaagttcg aagaggtgct tcatgacaat gctaatgctt ttatgtcgaa tgttatgact
    72121 cttgtatcta atgacagtta tcttgcagat agtgaaccga tgtctatcat gagtggtgcg
    72181 ttaactgctg caacattaaa tcttgggcta gataagaatt taggttatgc atatttagtt
    72241 ccattcaata gtaaaaacaa gcaaacagga aaatgggaaa agaaagctca attcatgctt
    72301 ggctataaag gatatatcca attagcccaa cgatcaggta aatacaaagc attaaatgtg
    72361 atcgaagttt acgaaggaga actaaaaagc tggaaccgac tgacagaaga gtttgagttt
    72421 gatccaaatg gtagaacgtc tgatgaagtc attggatatg ttggctattt cgagttactg
    72481 aatggattca aaaaaactgt ctattggacc aaacaagaaa tcgaagctca tcggattgct
    72541 aacaataaag atcgcgataa gacaaagtta agtggtgtgt gggcatctga ttacaatgca
    72601 atggcacgaa aaactgtttt gagaaatctt ctttctaaat ggggaatctt gtccatcgaa
    72661 atgcaagaag ccaccacatc ggatgagaga gtccaaaggg ttcaagaaga cggcagcatt
    72721 attgctgaaa cagaagttga agaagatatt cctgaaagaa aagaagcaga ggttatatct
    72781 gaagaaaatg aagatgtaca aactggatta tttgatgcat ctaatccgcc gttaaacaaa
    72841 taatgaggga gctttctccc ttgcttttct agaggagaaa tacgaatgaa cacaggatat
    72901 gtcaaattac atcgaaaagt gatgaattca ttcgtttgga ccaatcctta tatgtataaa
    72961 ttatggaact tgtgtttgat gaaagcgagt catgagaatc gcaagatact ttttaatgga
    73021 aaagagatac aagtgaacag cggagaattc gtcacagggc gcgatgctat cacatctgag
    73081 atgaacgaag gcgtgaaacg tgaacatcaa gtgaacagcg gttctgtatg gagatggcta
    73141 aaacaatttg aaaaaaacgg aatgttgaac atcaaatcaa ctacgaaata cagcgttata
    73201 tcaataaaaa actggtcttt gtaccaaagc agtgaacaac aaatgaacat caaacgcaca
    73261 acaagtgaac aacaagtgca cacaaacaag aatgaaaaga attataagaa tgaaaagaat
    73321 aataaaacat catcgtcgca accacgcaaa aagcgtgttt acgacaccga ctcagtttac
    73381 tacattctcg cggaggagtt attcaaacag atttgtcaga atcaggaaat caaaaagccg
    73441 aatctgcaaa gttgggcaga taacattcgg aaaatgatcg agatcgacaa gcggaccgaa
    73501 aaccaggtac ggggaatgat tgaatggagt cagcacaatg tgttttgggc atcgaatatt
    73561 ttatctgcta aaaaattgcg agaaaaatac gacacaatgg cagcgcaagc caatcgtgat
    73621 tataaaacaa aacaaactaa aacgcttgaa tacgagaaat ttagcacaga tgagttgcct
    73681 atttgagagg aggcgtaggc atggaaactg ttggggaaat catggaaaag ctgatacaga
    73741 aagtgcttgt ccaacgtggc gaatgtcctg aatgcggaca gcctttgtat ggatggcgta
    73801 cgaagaaccc tgatggctca gaacgttgta agccaacctg tatgcaatgt gggtataaag
    73861 cattgcgagt tcaggaagac ttacaaacag aacgaattta caacgagagc ctgaaagcaa
    73921 gagcaatcaa ttttttcaaa ggtggttctg ttgtgcctaa tcaagcgttg tttgattgca
    73981 cattgcagaa ttatcaaatt gtcgatcaag aaacaagaca agcggttgaa gtaaccaaac
    74041 gctttgttaa ttcagtcttg ttaggaaatc caagtcacct tgttttaact ggaaaacaag
    74101 gaacaggtaa aagtcaccta gcgatggcag cggcttggga agtcttgaag cgatcaaact
    74161 acgataaaaa aatcttattt atcgggttac aggaaatgct ggatcaaatc aaattttcgt
    74221 acaacaatcc tgaactcaga aaaacgattg agggatcgtt gattgcagat atcaaaacag
    74281 cggatttagt catcattgat gatatcggtt cagaactagg aaaagatgca tcagatagtc
    74341 gagcgtttgg cataaacacg ctaaattcgt tcttggatgc acgacagaac ctagcaacga
    74401 ttatcacaac gaacttgctt ggtgaagaac tgaaaaaagc ttacggtacg agaacaatat
    74461 caagaatgtt tgtcaactct gatggattta cgatggtatt ttctcaaaca gcagacaagc
    74521 gcataaaacc agtcaaagga gcgatcgcat gaatttgact aaacaagaaa aaagaatctg
    74581 tcgtcagata atgttgcaaa ttataggtga tcgtgacatg gacgaattat ctcaacaaga
    74641 gagtcgaatg gttcggaaga taagtaagct aattatttat cgggatacag aaagatttct
    74701 cactgtttta ggtaacgtct acgagaatcc agagttattg gaggtagagt aattgccaaa
    74761 aatattagat gcatgttgcg gtagtagatt gttctggttt gataagaaca attctgacgt
    74821 aacatttatg gattgtcgtc agcagtatga agaattatca acaggtcatg tgatcaatgt
    74881 tgatccagat gtggtcgcag actttcgcga tatgcctttt gaagatgatg tgttcgatat
    74941 ggttgtattt gacccgccac acttaagaca tgtcggcgaa aactcatggc ttgccaaaaa
    75001 atatggaaaa ttagacgaac tttggccaga agacatcagg caaggttttg cagagtgtat
    75061 gcgagtttta cgaccttcag gaacattaat ttttaagtgg aacgaggaac agataccact
    75121 ttctgatgtg ttagaggcta ttggagaaca gccattgttt ggtaataaac gaagcaaaac
    75181 acattggctt gtgtttatga aaaaattcag ctaacggagg aaacaaagga ggaaaagtaa
    75241 tgaagattgt attaaataaa tgttttggtg gctttgggtt gagccatgaa gcaaaaatgg
    75301 aaatttttaa aagaaaaaat attgaagtat tcccatacat caataacttt agctatgact
    75361 cagatgatga atacacgaga tacacaggcc aaaagctagg ttcgatggat tttatctact
    75421 atttcaaaaa agacccgaaa atcgacaaag ttacaggaat ttatagcgaa attgaccgac
    75481 tgtacggaat tgctgatgat tccagttttt caagtgattc aaacagaggt gataaggacc
    75541 tcgttgctgt tgtcgaaaaa ctaggaagtg aggccagtgg accatatgca agtcttaaag
    75601 tcgttgatat acccgatggg gctgagtggg aaataagtga ttatgacggc gttgaaactg
    75661 cacattatgg ttttcaaaca ggtagtattt agtccactaa tgacaactaa agaggaggca
    75721 gaaggatgag tgaagatgaa tactttgaac agtagtctat taacgacaga gatttttaac
    75781 tggaaagggg tgtagtcatg gacgcatcgg aagtagtaaa taaaattaat agtctcaaag
    75841 aggttttcgg agacgtcgaa gtagtgataa acgcacaagg ctatgacaac ggatttttta
    75901 agcagattac cgacattagc attcagcaag gaatggaaga cgaagatgga aactttatcg
    75961 atgaagtagc cattttagtg acttgcgagt aagtaattga cagctaaaga ggaggcagaa
    76021 ggatgaaaaa attgcaccaa ctcatatctg agaaagaaag cgaactccaa aatttagaag
    76081 atagtttggg attggggttt ccaattgttg agcaagctaa aatgactcaa atcagtcatc
    76141 tacgacttga attggaagat ttaagacaaa tagaaaaatc aattcaactc aacgacaacc
    76201 agaaaatcgt gttggagtgg ttgaaaagcg agacaatttt aactagagaa gcaccaatat
    76261 tatctgttaa tgctttttct gataaaaatt tactaggaaa attacctgat aaagtacgca
    76321 aagcttataa actattggct tgtaaacaag aatatgaagt cctatcagca ttcgctcaat
    76381 ggggattaga acaggaggaa gcggaatgaa caacagacat cgcagaataa caaaactaag
    76441 aaaacaggaa ctgaatgtac taaagacaaa gtttgaaaaa gaatatggaa tttcagcaga
    76501 agaaacatat aaagtggcaa gtcagtttgt tgctgatgcg agtaatgcta ttcgtaagtt
    76561 tgggatttcg atattaaatg atgatcgtaa atgggaggaa atgagatgaa aataaaagac
    76621 ggattttacg ctagtagtca tggtatcggc ggtttaatgc tagacatgcc gataaagaac
    76681 cctaaaacac gtcagaaatc aaaattcaaa atcggtgaca tggtccgatg taaagcagaa
    76741 gagttcatct atccgtttcg tggatatgta gagcatgtct ataatcattc agcaatcatt
    76801 cacattgaaa acacgatgga atgcgataag tggttagcga aaagcaaaga taatttagca
    76861 gtggctcgtt tggtggatat ggaactaatc aatgacaaat aaaaaagccg gatcgctccg
    76921 actgattcaa taaatccaac acatttatta tatcacataa aggagcggtt tgacttgatg
    76981 caattgttac gagaggtaga tttcaaacag acaagatgta atgcgagaga tgtgctgaag
    77041 aactttcggc gtttggagcg gatggcaggt cgctctttga tagatattaa gtcgccgatt
    77101 attacggata tgccgaaggc accgaagcac ggcaataagg cagaagacgc gatcattcag
    77161 atgatggata tagaagcgga gagagacgca atactagcgg ctttgatggc tcttagtctg
    77221 attagccgtc agatactcta ctacagcttc tgtgacgtaa acaagcactc taattatgaa
    77281 atagggcaat tgatacgagg atacggagag aagaatgtag agaagttgaa atccatcgca
    77341 ttaatcgaat ttgcagaagc atacaaaaaa ggcgtgttag ttcagtatcg ttgattttgt
    77401 agggtttttg tagggatagt gtagggtttt tgagcggttt aacgtgatat tatggtagtg
    77461 tcgaaagatt agtgataggt ctgagacaaa ataaaatgta agggaggaaa tctccctcat
    77521 cgttttaact taagcttcga tagacagcaa cggaaatatt aagaataagg atgtgaattt
    77581 caactccttc taaattgttc ttattatcta tcatccgttg ttgtctatta atttatgcat
    77641 cggaggaaaa acgaatggat aaagaaatca aagtaactgt caaattagat ttgactgaac
    77701 taaaagaact gctcaacaag gctagtgacc aagtcgaaca gttacaagaa actttagatg
    77761 aaattgctaa ttttaaaatc caagtttctt agcagtatat tttttagcag ctgcactcat
    77821 aaaatcatac caagtatcag aaaaatataa cttaactaaa taattaaaaa aggtgggtaa
    77881 atagaaatga cggcgttaat aatttcaatt tttgcgctct gtcttaatgt ctatatgatt
    77941 ggatttaaaa atggacaaaa taaaaaatag tagcagccga gaataatttt atagtgtcac
    78001 tgtggcggaa atagaagacg caaaggtgga ggtaggaagc gtatggcgca ctatcgggac
    78061 cttgtacaaa actagaaagg actagtacgt gtgtggtgcg agccctaggg aggaacagga
    78121 taaccgcctg tgtgtaggtt gctattacat attagatcac tctttgagtg gtctttttat
    78181 ttacataaag gaggctacat aatgagaaac tactggtata tatcgctaac taatgaatat
    78241 cctcgaacca ttgatgattg ttcagtgcgt gttgtgcgtt ctgtccaaat caaaaagaag
    78301 tactccattg ttgaaatgac cagagaagct acgccaaaag agattgataa atgcaaactt
    78361 ctttattgcg gtcatggttt ctatgatgaa ccaaacattc aaaataatat taacaagaat
    78421 ttgagggatt agaatgcaag aaatggctta aaaatctcca atcaggacaa atcaagttag
    78481 acaaagtttc cgatttaaag atattgattg aagctgattt aatgctaaaa gatattgaaa
    78541 attagaaaac aaaactcaac ctaagaagat tgcgaggtgg tgtgtattga atggcaagac
    78601 aacgtgatcc aagacgtgat gaagccaaaa gaatttggtt agaatccaac ggagaaaagc
    78661 agttaaagga aattgcatct gaattaaatg tttcagattc tcaggttaga aaatggaaat
    78721 cgcaagacaa atggagcgct gaattgaaaa gtaacgttac caatggcaaa agtaacgtta
    78781 ctaatcaagg tggcgctcct attggtaatc aaaatgctaa aggtaacaaa ggaaacagca
    78841 gagcatcacc gccaaagaga aataagaatg ctcttaaaac tggtgagtat gaaacaatat
    78901 tttttgacac gttaaccgat aatgagaagg acatctattc tagtttgaat gatgatcctt
    78961 cttttgcttt gtccgaagaa atacgacttc ttaaaataag acaacttcga atgatgaaaa
    79021 gaatccaaca agctgaagct ggactaaatg atgaagaagt cgaacgattg cagcagctaa
    79081 gaaagattaa aactccgatt gaaaaaaatg gtaaaaagct agaaatcaag cgtgaggtta
    79141 tgcaagatgt gcagattagc agaaaaaaac atcgcaaaat tgatgatatt ctttcaattg
    79201 aagattcatt gactcggatt agcaaccagt tagctaaagc catcaagcaa atgaatgaac
    79261 tttatatgaa tgaatacaga actgatttaa ttaaagctca gactgataag atccaagctg
    79321 agacaaatga aattggcgga aataattcag gtgaagaaat agaagaatgg aaacaggcag
    79381 ttttaaatgc cgcaaacaaa cgggcggtga aagaaaatga ataatgaatt tattcctttt
    79441 gctgatattg gttctgccat tgattattac tatgataaac cagtagcttt ttgccaagat
    79501 attttgcatt tgaatcctga tgaatggcaa gaaaatgttt taaatgattt agctgaattt
    79561 tcaaaggttt ctgttcgttc tggtcaagga gttggaaaaa cagcattaga agcaggagca
    79621 atactttggt tcttaacgtg tcgaccctac gctaaagtaa tagcaacagc tccgacaatg
    79681 aagcaacttt acgatgtact ttgggcagag gtagctaaat ggttaaatga tagcttgatc
    79741 aaaaacttac tgaagtggac aaagaccaaa atttatatgg ttggtgattc agagcgttgg
    79801 tttgctacgg ctagaacagc gactaaacca gaaaatatgc aaggttttca cgaggaccat
    79861 atgttgattg tggtagatga agcttctggt gtgtctgatc caattatgga agctattctt
    79921 ggtacgctat caggttttga taataagctg ttgatgtgtg gaaaccctaa taatattgaa
    79981 ggtgtctttt acgattccca caattcagac cgtgataaat acagagttca taaagtatca
    80041 agctatgata gtaaacgtac aaacaaagac aatatagaaa tgattcttaa aaaatatgga
    80101 aaagaaagtg atgttgctcg tgtccgtatt tttggagaat ttcccaaagg tgcgttggat
    80161 tcatttatca gtcttgaaac ggttgaattg gctacagaaa aacaaattag tgattcttta
    80221 gtcaataaaa caacggttgc tcatattggt gttgacgtag ctcgatatgg tgatgattct
    80281 acgattctct ttcctagaat tgctaccagg gcattggagt atgagaagta ttcaaaacgt
    80341 agcactatgg aaacaacagg atatgtcatc aacatggcca agaatctaat gagtcaatat
    80401 ccgagtattg ataaagtgat gattaaagtc gatgacactg gtgtcggagg tggtgtaacc
    80461 gaccgcctag aagaacttat agaagacaag cattatcctt ttgaggtgtt tggagtgaat
    80521 aacggttcaa catcagaaga cgatttttac gataatttag gtactcaact atggggaaac
    80581 atcaaggaaa tgttagaaga aaatatgaca gcaaatctta acggagaaca gcctgttatt
    80641 gaattgcctt ctgatagttc gttaatcaaa gaattaagta ctcgcaaatt caaaatgaca
    80701 agtagaagtc gtatacgttt agaaagtaaa gatgatatga aaaagcgaaa tattggtagt
    80761 cccgatattg ctgacgcact ggctttagcg ttttatgagc caccaagtca ctatcaattt
    80821 attcaatttt aggaggtgag tttttgatta gtacagtatt atctttagag agatataaga
    80881 agctacgtgt taaatatgca acacaaattg aagatggaat gtttgatccg aatggtttta
    80941 tagaatatat gaaaccattt tttgctgatc gcgaaagaaa atatctagct tatactagcg
    81001 aaaagaatga aatagacaat agaccaaagc ctaacactga tattgtaaaa gttaataata
    81061 aacttcatgc tggtatgtat tcaattgtcg tagaccaagc agtcaatcat tttactggta
    81121 tacctatcaa atgggattac gatgtatcgg aacaaaaaag aacactcatt caaagattaa
    81181 aagataaatt cttaaaaaat gatatagaac ttccaacagt tccagaagct tttaacaaat
    81241 taacaagcaa tcttgattcc atgagattcg caatgcttga ttctgaaact gcaacttttc
    81301 aaggagcttg tggagtagct ttccgattgt tagagcctgt ggaagaagat gatggttgga
    81361 agttaagagc aagtaatatt gaaccttgga gagcagaaag atatggaaat gccggaatct
    81421 atatcaaaga aaagtatgac tcttaccaga aaaaattttt tcaagaaatg aaagttatga
    81481 caagaaataa aattctgaca tatgcttgct atggtgattt gaattttgtt acaagcggaa
    81541 cgttcaaaaa aattgatgag accgataacc ctttggggac gattaccttg gcagaattta
    81601 aaaataatac gaatcgttat tgtgattttg aagtagctga ggaaattgga gatgcaattg
    81661 atcgtgcttt gtctgatcaa caaaacgaaa tcgaacaatt taagcttgcg tacatgcttg
    81721 ttactggcac aacaatgagc aaaggcactg ctaaagaaat gatgaatcag ctagggatta
    81781 tcaacttaaa agaccctact gcaaaagctg aatatgtcac aaaaaaatta gcaaaagatt
    81841 tcaatgaata tcatatggat ctattgaaaa aacagtttta caccatttgt aaagcaatcg
    81901 attttaacga tgaggtgttt aaatcaaaca gttctggaga agctcgcaag tggcaaatca
    81961 ttagcctaga agcgaaaaca aatactaaag agcaatattt tagagaagga ttgaaagaat
    82021 gcgcagaaac aattgctgct tttcttaagt ttcatgacaa agtagaaatt gagcctgaaa
    82081 aaattatttt tactttctct agatctttgc caactgattt aagttattta gctgaagctt
    82141 tacctaaact tgcaccatac gtatccaaac gaactattca gagccaaatt ccatttgtaa
    82201 cagatgttga ttatgaaaat gaaatgatgg aattggaaag tggaagtgct tatccagata
    82261 gtgaatacaa ttttggcgga ggtggcaata gtgacgatag aaacgaagta ttggaccaaa
    82321 cgtcgagaac tggaggatca agcaaggctc aaacaagaaa atcagacact taaaaaatta
    82381 actagtgtat ttcctgaagc actgaaagag atacaagcaa aactattatc acaagctgac
    82441 ttacacaata tcacttatcc agaaatgatg gagttttaca gtacaagtaa tcagaaaaaa
    82501 tatcgtgaat atgtggaaaa aaattataag tcattaaaaa tgtatgatgc aaaatacaaa
    82561 gagtttatcg atgaattttt tccaccattt gactatgcaa aagtcaatcg cttattacaa
    82621 atacgatcag atgtatttaa aattcttgca gaatatgcga tggatgcaga tgtgaatcaa
    82681 tatttttctg atcgcttaga ggaaattctt caaagaacat attcttctaa tgctaatgtt
    82741 tttgttcaac ttttaaacgt tgatatacca aattatttac cagaaaatga aattaaacat
    82801 tatttgaact atccatggtc aggaaagaca ttttcaagaa gactttgggg aaatatttca
    82861 tcgcttgaac agaaactctc taatgctatt gtaaaaagtg ttgctagtgg agaaggcgtt
    82921 atacacgcat taaacaccat gagattagac tcagaaattt gtgacatgtt taagttagaa
    82981 gaatcaaagt ataacaaagc gatagaaaat ctcgttcgaa cggagtatgc aaaatttgca
    83041 caagatggta ttgaaaaatc atatttagaa acaggtattg aggaatacaa cgtattgact
    83101 gcaaaagatg agagagtttg ccggatttgt ggaggaaagg caagtaagaa tccctataaa
    83161 ctgaaagatg ctgtcatagg tgaaaatcga gcacctttcc atagtcgttg tagatgtacg
    83221 gatgttccta atttaccaaa attaggaaag gatattgatg aagaatatga ccgtttattt
    83281 ggcgatttat tagatgagtt tgcacatgat tcttttggaa ttaatttgaa acggaggaag
    83341 tagaatgaaa gatttttttg aagcagtact aacaattaat gtaaatgctg atattgcaga
    83401 agcctacaaa acagctattg agtctgagaa ccatcctaat ggcttgagag accattggaa
    83461 tggcaattat gcctacgtgg ttattggcga tcaaactgtt aattatcaag ataatactcc
    83521 agttgataag aataccgtta atttaacgat tcaattatta tctcattcat taccaaattt
    83581 aaaagaaaca gttgattggt atgaaaagat gggatgtatt gttgttagaa ctgactacaa
    83641 agaaggaaag tctagtaatt aggctttttt attttgtccg aaatgacgtt aaactagcgc
    83701 aatactgggc ttggttgaat ggtggggcgc aactattaaa actcaaagca atgcggggcg
    83761 tgaaaacgaa tcgtggggcg aaaggagaat gagcatgaaa cacaaatcat taatgccaat
    83821 gaatttgcaa tactttgccg aaggtgacga tcaaaagttt tcttttgatg acttcaaatc
    83881 ttttgtggaa tccaatgaag aagctcaaaa atttattcag tcacaatctc aaagtgttgc
    83941 cgacaaacaa ttggaagctt ggaaacaaaa taacttagat aaaattaaac aggataccat
    84001 caaggaatat gaggaatcta agaaaaacaa gtcacctgag caaattcaac tggaaaaact
    84061 acaagctgaa tttgaagcag aaaaagcgtt gcgtgtgaca agtgataata aagcatttgt
    84121 tgcagaacaa attgctggat tagaactaga tggagagtta aaagagtcta tttctcaatt
    84181 tatgctaaat aatcttgtta gttcggatac agatttcact aagaatgctg ttgaaggttt
    84241 tacaagtgtt ttgaatgcaa ttaatgagaa acatgcagat gcattaaaag aactacaaat
    84301 gaagtctgca tttggtggaa ctcaacaatc gaataaccag gttcagcaga acaatgaaac
    84361 attaacaaat ccagaagaac aattaggaca aattcttcaa caatttaact aggagagtga
    84421 aaaattatga aaaaaacatc tttaaataat ttagagtatt tggatatttc accagcgatt
    84481 aatgctatgc aagtaccaaa tacacctttt ttaagctatt tacttggtgc tggaaaaaca
    84541 gagccagcaa actcgacaga aattaaatgg cgtgaatatg atatcaacaa cgatgattct
    84601 tctgaaaaac ttgagggcgg agaatatcca gatgctgaat caggtcgaac ttggtttaac
    84661 aactatactg aaatttttag aaaatcaacc tctgtatctg gtacattaga tgctattaat
    84721 gtgaatggag tcggaaatga attaactaat caagtagcac tacgtggtat ggaaatgaaa
    84781 attgatttga accgaaaatt gattactggt gtaaaagctg atgaaaatgg ttctaaaggt
    84841 cgtcgaatga atgggatttt gaacttgatc aattcagcaa ataaggcaga aacagccact
    84901 gcgggtgcag taacaagaaa agatatcgat actttattta aattgatgta tgaaaaaggt
    84961 tatatgggag aaaaactatg cttgatttct ccagatatgc aggagttaat gactgatgag
    85021 ttagatggaa aatcaacaaa aattgttcag ttcggggaaa gagtaacttt tggattgcaa
    85081 attggaaata tcgtgtctaa ttacggtaca ggtattgctc tactagaacc atcattgcca
    85141 aaaggaacaa ttgccgcaat cgatactaat tatgtgaaac tacgtccatt acgtgaatgg
    85201 agagcagaag aacttgcaaa aacaacggat tctagacgta tcggtcttgt aggtgaatac
    85261 tctcttgaat acaatgcttc aaactctggg gcaattttaa atttaaagtc tgaataaaag
    85321 ggagttagta ctcccttttt tgataggagg aattacagtg gtaaaaaaag atgaaactaa
    85381 aaaagatgaa gtcgtgaaat atagagtagg taaaactaaa aattttgttg ggtttgttca
    85441 tcctaaaact cgtagattta tcacagcaga ttcaaataac gaatttatca tttctataga
    85501 tgataaagaa gcaattgcaa ttttggaaga tgcaattgat gttaatgaaa tttaggaagt
    85561 gatctgatgg atgaatcgct aaaaacggaa atcattgagt ctacaaaaga agattttcca
    85621 gatttgagtg aagaacgcat aactaattta ttagaaataa ttttgctaga aattgaatca
    85681 tacaacactt gtaaaaatga tatttcatgg gaaaagttaa aaagcgtgat tagtgaagtg
    85741 ttgtataaaa taataaaaaa tgaatcagaa aaaacagtat cttcagttag acgtggcgat
    85801 acaacgatta gttatgcttc aacaacaaat gatgttagtg agttacttct aggctatggt
    85861 gatttgatac gaagagttat tggttgtgga ggattggagt ttttttaatg aatgaagcag
    85921 atattttaga aactacttat gaagatagtt gtattattga aagactgacg gacattgaag
    85981 attctaatac aaatattact attcaagatt acaaaaaagt atatgataat cctatttctt
    86041 gcgctctttc acaaggtcaa atcgatggac tagcagtcat agaagatgga gagatggtaa
    86101 atgtttcaac tgacacatat aaattatttg ttcatcctaa gattaaacta aaaaaaggag
    86161 atcgaataac aataactcaa aaggccagtg gcttaatttt ttctctattt gctactaagc
    86221 ctttttacta tcctagtcat tgtgaagtga atttgatagg aagtgagaaa aatgggtaat
    86281 ctcaaatatg aatcaaatgc agaaaagatc attgagaatt ttaaaaatat gactgtaatt
    86341 gctcaaaaag aaggaatatc cttcgttaac gattcaatga ataaagtcgt tagtttagct
    86401 aaaccattaa ctcctgtaaa atcaggtaat ttaaggcgag gatatcgtgt agtaaaagct
    86461 agaaaactat caagcggtcg aattgttgga gcagttataa acaatgaaca ttattttaaa
    86521 tatgtagaag aaggtcatag gactaaaaat ggtggctttg tcaaagggaa attcatgtta
    86581 actcgtgcaa cgaatcttgc aaatatgtct tatattcctc gaagatttaa acaaatggca
    86641 ataaaaatcg ttaagaaagg aaagtaacat gaaagatgaa atcattgctg caatcagtag
    86701 caaattaaaa gaaatctatc ctgatgggac aatatatctt gattcagtta tgcagtcaac
    86761 taaagatttt tatttcgttt tatcagtaat ggaatctgga actgaaaatg taggaattga
    86821 tattcaaaat gtttctttct taattgatat tgcattgatt gataataaac ctaatagaaa
    86881 tttaattaat gaattagtct cacgttgtgg gacttttttt aatacgatta caatcgatga
    86941 gcaaatatta tttccaaaag cctatttacc tgatgaagca gatggtgttc aacatattcg
    87001 ttttacatta gaatttccac aatatattga atggagtgaa agataaatgg gagaaaaaag
    87061 aagtaaaacc ggaattattt ctgtcgaaaa acctacttgg tttcctttgg aagatgagac
    87121 agggaatttt ccggtatatg gtactgcgtt gccaatggga acagcagtaa gtatcaaacc
    87181 aacagctaat tatgaaacaa cacaagatta tggggattca gtggttcaag atcaatttac
    87241 agcttttggt ggtgcagaag ttgaactgga agcaaatgga tatactcata aagttttatc
    87301 tacaattact ggagggaaaa ttgttaaagg tggtgccttg cgttctggag aagatattgc
    87361 gcaagatggt gcgtttgcat atagacgtaa aaaatcgaat gggaaatacc gttatacggt
    87421 cttttataaa ggacagtttg ctctagattc agatgaaaca tcaacgattg aaggaagtaa
    87481 agtaagtttt actcatccaa cttggaaggg ttcatttgta gatgtaccag gacttggata
    87541 tatgtattca gttgatgaag atgatgaagg cgtggataaa gcaatgatcg gaaattggtt
    87601 tacaaaagta gcaattccaa ttgaagaagc agaactttca ggaggtacag aataatgtca
    87661 aaatatcaaa caacgattaa attaacgaaa aaagatgaag aaggtaaata tgagcaagta
    87721 caatttaaat ctgctgaatt tctaccaggc ccagtcgtag aagaagcagc agaagtaatg
    87781 gagggaatgc aaaatgctgt agatggaaaa tcagtatcag aagcattaag tcgagcttat
    87841 tcatttattg cagatatttt gtttgaagga cagtttactg gtgaagatta tcgtcaaggg
    87901 atcgatgctc gagaaattgc ttcgttgaca ggtaagttat taaaatctgt tactgcaggc
    87961 tttgatgaaa cttatacaga aacgaaaaaa aagtaagcga agctctcaaa tatccttttt
    88021 ttaaacattc gattacttat cgagaattag atttaaagac gcaattactc gaagcaggtt
    88081 ggacgttacc agaaattgaa cgtactgact ttgacgagtt aatgcgtctt tttgcttttc
    88141 gagatgcagt aaaagagcat gaggatgttg agtactacga taacttcacc caattttagg
    88201 aggtgatact ttgaataacg atgacctaat tctgaagatg atactagatg aatctggttt
    88261 tactgccggt atgaataatg ctgtcaaaaa gttgaactct tttgatgaaa caatcgaaag
    88321 aaaaagtaga aatagtggca gttcattagg tagtatttgg aaaatatttg ctggaagttt
    88381 tctagctagc ggagtaacta gaatcgtagg agctggcttt gatctaatca agggttccat
    88441 aagtggagcg gttgatcgag tagatacgat gaataacgct ctacgaaatt tccaaaatat
    88501 gggatttagt aattcagaaa ttatgaagaa tatcggaaag aatgggcttt tatctcaagg
    88561 tattcaagga cttcctactg ctttgaatga tgcgataagt catgttcaac ttcttgcttc
    88621 ttctacaggt gatttaactc gttcaactca aatatttaaa gctttaaatg atggaattct
    88681 tggtttcggt ggttcaactg atcaagttaa tgaagctgtt attcaattat ctcaaagttt
    88741 ctcaaacgga aaagtagacg cacaaacttg gaactcaatg atcaacgctc aacttggacc
    88801 tactctttct gctattgcta aaaagatggg aattacaatg ggagatctga aagaaggttt
    88861 atctcaaggt aagatttctg ttgaagaatt ccaaaatcaa ttaatagaaa tggataccaa
    88921 aggtggcgga ggacttaaat cattaagtca aatcgctaaa gattctacta agggaattaa
    88981 aacctctata caaaatgcaa aaacagctgt tacacgtggt gttggtgagg taatagaagg
    89041 attaaataaa gcattagtag attcagattt aggtggattt aagggaatta ttgataaagt
    89101 gggcagttca atggaatcgt tcttaaaagt aattgctgca aatattccta cagcagtatc
    89161 atttttaagt aatttattta acgaaattca aaaatttggt tctgctttga aattcatgat
    89221 gccctttctt gttcctgcag ctaccgcttt tggagcgttc atgtttcaac ttaaaggtat
    89281 accagcaata ataaaaagct tcaataattt taagaatgcc ataattggcg ttggaaattc
    89341 attaaagatt atgggggcaa tagctgctgc taattcattt gttttaatta ttggtgcagt
    89401 cgttggtgca atagctgttt tcggttactt tatggcaacc aatgaagagc ttagaaacaa
    89461 ggttatatct atttggaatg atgtgaaaga ttccgtaatt ggtgtattaa aaaatataaa
    89521 ggactgggga attgatactt ggaattctgc taaataaatg gcatcaaatg cagttgaggg
    89581 tgtcaaagat gcttggtcag gaacaaagga atggttttct aacacctgga aaggtattaa
    89641 ggatggggcc acaggtttat ttgataaaac agtagagact tcaagaaatg cagtcgatag
    89701 tgtaaaaaat gcatggtcta atatgaagaa atggttttct gatacttggc aaagtataaa
    89761 agattcagcc agagaaaaat ggaatggaat caaaggttct attatgtcag ttgctggtcc
    89821 attgattaca ggtataaaga atgcattttt acatgttact ttttatctag agaccttatg
    89881 gaataattta gttgagattg gtaaaaacgt ctttgagatt ttaaaaaatg ttatacttgc
    89941 gccggttttg tttattacct ccatgatttc tggtggatgg gaagaaacaa aaaataatat
    90001 gattgctgtt tggaataata ttaaagaaag tgccataaat atttgggaat ctataaagaa
    90061 tatatttgta agttatttta caaatattta ttttgctgca ctcaatattt ggacagggtt
    90121 caagcttaca ttgataaata tttggaatga agtggtaaat caagctaaat cgatttggtt
    90181 aaacataaag tattttttta ttaatctttg gattgatata aaatattttg caattcagaa
    90241 atggattgaa ttgaagtttt ctattattca aacttggatc gatttaaaat ataatgccat
    90301 tactacttgg aataatatta aacagttttt caaagatact tggaaaaata ttaaagatac
    90361 ggcatacaat acgtggattt ctataaaaaa taccatgatt aatacatgga ataacattaa
    90421 ggactctttc tggaatattg ttactggaat tgttaattct gctgaaaatg catggacaaa
    90481 tctaaaaaat ggtgtttcaa atgctattaa tcgggtaaaa gaaatcttta attcgttaag
    90541 ggaagtcaat ttatttgaaa ttggcaagaa catcattgat ggacttataa atggtgtaaa
    90601 agaaaagtgg aatgctttga aagatactat caaaggtatt gcaggtaata tcaaaaattg
    90661 gattaaagga gctttagata ttcattctcc atcaagatgg atgcgtgata tggttggtaa
    90721 aaatattgtt caaggtatta tcgttggtat tgataaagag caaagtaagt tagatcaaac
    90781 catgacaaat ttagtaaaaa caccgtctgt tcaacctgta gtaaatggaa taagtcacct
    90841 accaattaca caaacaaagc aaaatatatc tgctagtgaa acaaaagaaa ttcatttaca
    90901 tttaaatgtt tatggagatt tacctgattc aatggttcga aagattacta caaaaattaa
    90961 aacagaattg acaagacaaa tgaaacgaga tgctggtgca gtaggaggaa cattatatgc
    91021 aacttaagag aggacaattt tttattaatc aacattattc ttctgaattt aatgtgtata
    91081 ttcaaaacag gcctgcttct gtttcagcta gtcgagtaat tgaattgaga gagcgagaag
    91141 gaaatgattc tatcattatt gataaaggct attacaaaaa tgtgactaga aagattgagt
    91201 gttactataa agcaccttca attgatatgg tgcaggagtg ggaagatcga atcactgaat
    91261 ggttagatat gtcgtcttat agtgatttta ttctttacta tgatgaacaa tatatttatc
    91321 aagctgtagt aatagaagga cctgaattta aaggaacaag aaaaacagga aatattgtcc
    91381 cttttgaatt tacagtgagt attcgaccat ttaaggaaaa ttacaatggt agatttacta
    91441 ttcgacaaac agaaactttt gaaatttata acccagagaa gtattcttca aaaccgctta
    91501 ttaaattgag tggttctgga gatgcttctt tttatattaa taaggataaa tatgatttga
    91561 aatcgttgga cagagaatta tttatagatt ctaaattaga agaggcttat cgaaaattag
    91621 atggtaattt agaacatcag gatcaggtca ccttattttt agattttcca tttttatatc
    91681 ctggaaaaaa tgaaattaaa tggacgaaaa atattcattc atttgaaata atgcctaggt
    91741 ggtggagaaa agtatgaaac caagaatata tagtcctact gaaacagatt tctcaacgaa
    91801 tggcttagga attttaaagg attgtacaag atgtgaaata tatgaagtag ctaatggaaa
    91861 atatgaatta gagttggatt atcctttagg aactagattt gatgaatatt ttgaaaatga
    91921 ctatcaaata aaagcaaagc caaacgatca agaagagtat catatctttt ttattgatga
    91981 taaagatata gatacttttt taaatacagt aactatttat gctcagagtc gtacaaatcg
    92041 acttggaaga cgggtagtca ctcttgtgga agtggactct aaaactggtc aagaagcgat
    92101 atcaattatt gaaactaaaa tggataaaaa atctgacata cgactttatt ctgatattac
    92161 ggctgtttct agcacaatct ttgaagcgag aaatgtttta aattgtattg ctggtgaaca
    92221 aggatcattg cttcaatatt ggggcggaga aattaaacgt gaaccattta agctttcttt
    92281 gttaaagcga agaggtcgca ataatgttgg aacgattcgg tatggaaaag atatgtctgg
    92341 tttgaaggtc aaattagatt ggacaggtat aaaaacaaga attattccat atgctgatcc
    92401 tcaaagtgaa gtaggtacga ctaatcgaat ttatggttcg ccagtagata gtgcctatat
    92461 taataactat cctgatgtct atacagaaca tgttcagttt acagaagaac aaggagtaaa
    92521 agacattaag agcttaaata aaatagctaa aaattacttt aaaacgatca atcctggctg
    92581 tgataagcct aaagtttcta ttacggttga atttgataag ttgactgata gtgaagaagc
    92641 gaaagaattt gcgaagatta gaaattatgg tctgtttgat acatttaaaa tatatcataa
    92701 aaaatataat atttatcttg aatcaaaggt tagtggacta caatacgatt ctttatctga
    92761 aaaaactttg aaattagaag caggagacat tcaagttgct ttttatcagc aacaagctgt
    92821 aactattcag gataaattaa aagattatgc aacgaataat tatatgagtg attttaatga
    92881 ttatgtttct tcaatgatta caggtcaagg cagtgcagga ggatatgtag ttttatggcc
    92941 aaaagaaaaa ccttccaaca tctttattat ggataatcca gatttagaaa aggcaagaga
    93001 agtactaaga atgaataaaa atgggattgc tttttctaag aaaggatggc gggggccttt
    93061 taattcagct tggacattag atagtatatt taatgccaat tttatacaaa ctggattaat
    93121 caaagcagat atctttcaaa attcttttaa caagactgga gatgtattga aattagttaa
    93181 tggactactc caaatttgga acaacaaaaa gaaaataatg gagttaacta aaaaaggaat
    93241 ggaattttgg aattctaagg gttcaattgg aacgattgga acaactgatt ctgttggtaa
    93301 tccttttcct ggggcttcta ctccaacccc tattgaagat aattctttag ttattcgtac
    93361 aaatggagac ggcaaatata ttttgatttc tcctaaagta ggaaaaggat tagttttatt
    93421 aggaaatggt aaagcaattt attttggaga cttagatgta caaggaaaac ttacagttaa
    93481 aggaaaagaa ataattcctg gacaaggtgg tggtccatcg ggaggtggag aatctactgg
    93541 tgggtatcct agtgaattaa aaacagatgc agaaaagaga gcttggagaa t
//
LOCUS       DAZBTF010000002        88044 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000002 DAZBTF010000000
VERSION     DAZBTF010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 88044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 88044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..88044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.002"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(554..2167)
                     /locus_tag="ACHGNS_000108"
     CDS             complement(554..2167)
                     /locus_tag="ACHGNS_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFN6936094.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2308..3216)
                     /locus_tag="ACHGNS_000109"
     CDS             complement(2308..3216)
                     /locus_tag="ACHGNS_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN6936095.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            3399..4523
                     /locus_tag="ACHGNS_000110"
     CDS             3399..4523
                     /locus_tag="ACHGNS_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFN6936096.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            4750..5667
                     /locus_tag="ACHGNS_000111"
     CDS             4750..5667
                     /locus_tag="ACHGNS_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6936097.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            5660..6493
                     /locus_tag="ACHGNS_000112"
     CDS             5660..6493
                     /locus_tag="ACHGNS_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6936098.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            6576..7694
                     /locus_tag="ACHGNS_000113"
     CDS             6576..7694
                     /locus_tag="ACHGNS_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFN6936099.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            7715..9586
                     /locus_tag="ACHGNS_000114"
     CDS             7715..9586
                     /locus_tag="ACHGNS_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFN6936100.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            9580..10803
                     /locus_tag="ACHGNS_000115"
     CDS             9580..10803
                     /locus_tag="ACHGNS_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFN6936101.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(10889..12823)
                     /locus_tag="ACHGNS_000116"
     CDS             complement(10889..12823)
                     /locus_tag="ACHGNS_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFN6936102.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            13049..13705
                     /locus_tag="ACHGNS_000117"
     CDS             13049..13705
                     /locus_tag="ACHGNS_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFN6936103.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(13779..14132)
                     /locus_tag="ACHGNS_000118"
     CDS             complement(13779..14132)
                     /locus_tag="ACHGNS_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFN6936104.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            14324..15253
                     /locus_tag="ACHGNS_000119"
     CDS             14324..15253
                     /locus_tag="ACHGNS_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFN6936105.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            15264..16100
                     /locus_tag="ACHGNS_000120"
     CDS             15264..16100
                     /locus_tag="ACHGNS_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFN6936106.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            16348..17124
                     /locus_tag="ACHGNS_000121"
     CDS             16348..17124
                     /locus_tag="ACHGNS_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFN6936107.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            17300..18043
                     /locus_tag="ACHGNS_000122"
     CDS             17300..18043
                     /locus_tag="ACHGNS_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6936108.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            18036..19616
                     /locus_tag="ACHGNS_000123"
     CDS             18036..19616
                     /locus_tag="ACHGNS_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFN6936109.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(19867..20346)
                     /locus_tag="ACHGNS_000124"
     CDS             complement(19867..20346)
                     /locus_tag="ACHGNS_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFN6936110.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(20362..21114)
                     /locus_tag="ACHGNS_000125"
     CDS             complement(20362..21114)
                     /locus_tag="ACHGNS_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFN6936111.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            21231..21301
                     /locus_tag="ACHGNS_000126"
     tRNA            21231..21301
                     /locus_tag="ACHGNS_000126"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21263..21265,aa:Gly,seq:ccc)
     gene            21662..22387
                     /locus_tag="ACHGNS_000127"
     CDS             21662..22387
                     /locus_tag="ACHGNS_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFN6936112.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            22642..22908
                     /locus_tag="ACHGNS_000128"
     CDS             22642..22908
                     /locus_tag="ACHGNS_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFN6936113.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            22905..23336
                     /gene="ruvX"
                     /locus_tag="ACHGNS_000129"
     CDS             22905..23336
                     /gene="ruvX"
                     /locus_tag="ACHGNS_000129"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFN6936114.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            23361..23666
                     /locus_tag="ACHGNS_000130"
     CDS             23361..23666
                     /locus_tag="ACHGNS_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFN6936115.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(23747..24892)
                     /locus_tag="ACHGNS_000131"
     CDS             complement(23747..24892)
                     /locus_tag="ACHGNS_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN6936116.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(24953..25600)
                     /locus_tag="ACHGNS_000132"
     CDS             complement(24953..25600)
                     /locus_tag="ACHGNS_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN6936117.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            25794..26087
                     /locus_tag="ACHGNS_000133"
     CDS             25794..26087
                     /locus_tag="ACHGNS_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936118.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            26131..26442
                     /locus_tag="ACHGNS_000134"
     CDS             26131..26442
                     /locus_tag="ACHGNS_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936119.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            26439..27063
                     /locus_tag="ACHGNS_000135"
                     /pseudo
     CDS             26439..27063
                     /locus_tag="ACHGNS_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            27204..27362
                     /locus_tag="ACHGNS_000136"
     CDS             27204..27362
                     /locus_tag="ACHGNS_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936120.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            27363..27725
                     /locus_tag="ACHGNS_000137"
     CDS             27363..27725
                     /locus_tag="ACHGNS_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936121.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            27762..28610
                     /locus_tag="ACHGNS_000138"
     CDS             27762..28610
                     /locus_tag="ACHGNS_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFN6936122.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            28600..30075
                     /locus_tag="ACHGNS_000139"
     CDS             28600..30075
                     /locus_tag="ACHGNS_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFN6936123.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            30341..30748
                     /locus_tag="ACHGNS_000140"
     CDS             30341..30748
                     /locus_tag="ACHGNS_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFN6936124.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            30751..30966
                     /locus_tag="ACHGNS_000141"
     CDS             30751..30966
                     /locus_tag="ACHGNS_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936125.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            30970..31350
                     /locus_tag="ACHGNS_000142"
     CDS             30970..31350
                     /locus_tag="ACHGNS_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFN6936126.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            31484..31639
                     /locus_tag="ACHGNS_000143"
     CDS             31484..31639
                     /locus_tag="ACHGNS_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFN6936127.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            31708..32181
                     /locus_tag="ACHGNS_000144"
     CDS             31708..32181
                     /locus_tag="ACHGNS_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFN6936128.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            32178..33872
                     /locus_tag="ACHGNS_000145"
     CDS             32178..33872
                     /locus_tag="ACHGNS_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFN6936129.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            33838..34023
                     /locus_tag="ACHGNS_000146"
     CDS             33838..34023
                     /locus_tag="ACHGNS_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936130.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            34027..35202
                     /locus_tag="ACHGNS_000147"
     CDS             34027..35202
                     /locus_tag="ACHGNS_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFN6936131.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            35195..36718
                     /locus_tag="ACHGNS_000148"
     CDS             35195..36718
                     /locus_tag="ACHGNS_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFN6936132.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            36774..37058
                     /locus_tag="ACHGNS_000149"
     CDS             36774..37058
                     /locus_tag="ACHGNS_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFN6936133.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            37045..37380
                     /locus_tag="ACHGNS_000150"
     CDS             37045..37380
                     /locus_tag="ACHGNS_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFN6936134.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            37675..38301
                     /locus_tag="ACHGNS_000151"
     CDS             37675..38301
                     /locus_tag="ACHGNS_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936135.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            39043..39879
                     /locus_tag="ACHGNS_000152"
     CDS             39043..39879
                     /locus_tag="ACHGNS_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFN6936136.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLINLYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            39943..40374
                     /locus_tag="ACHGNS_000153"
     CDS             39943..40374
                     /locus_tag="ACHGNS_000153"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6936137.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(40436..41758)
                     /locus_tag="ACHGNS_000154"
     CDS             complement(40436..41758)
                     /locus_tag="ACHGNS_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFN6936138.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(41912..43552)
                     /locus_tag="ACHGNS_000155"
     CDS             complement(41912..43552)
                     /locus_tag="ACHGNS_000155"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFN6936139.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            43885..44355
                     /locus_tag="ACHGNS_000156"
     CDS             43885..44355
                     /locus_tag="ACHGNS_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFN6936140.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            44577..45680
                     /locus_tag="ACHGNS_000157"
     CDS             44577..45680
                     /locus_tag="ACHGNS_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFN6936141.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            45964..46644
                     /locus_tag="ACHGNS_000158"
     CDS             45964..46644
                     /locus_tag="ACHGNS_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFN6936142.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            46734..47768
                     /locus_tag="ACHGNS_000159"
     CDS             46734..47768
                     /locus_tag="ACHGNS_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFN6936143.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            47782..48117
                     /locus_tag="ACHGNS_000160"
     CDS             47782..48117
                     /locus_tag="ACHGNS_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936144.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            48101..50581
                     /locus_tag="ACHGNS_000161"
     CDS             48101..50581
                     /locus_tag="ACHGNS_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936145.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            50637..52196
                     /locus_tag="ACHGNS_000162"
     CDS             50637..52196
                     /locus_tag="ACHGNS_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6936146.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            52193..53557
                     /locus_tag="ACHGNS_000163"
     CDS             52193..53557
                     /locus_tag="ACHGNS_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFN6936147.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            53728..54798
                     /locus_tag="ACHGNS_000164"
     CDS             53728..54798
                     /locus_tag="ACHGNS_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6936148.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            54813..55481
                     /locus_tag="ACHGNS_000165"
     CDS             54813..55481
                     /locus_tag="ACHGNS_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6936149.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            55501..56079
                     /locus_tag="ACHGNS_000166"
     CDS             55501..56079
                     /locus_tag="ACHGNS_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN6936150.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            56480..57391
                     /locus_tag="ACHGNS_000167"
     CDS             56480..57391
                     /locus_tag="ACHGNS_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFN6936151.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(57769..58686)
                     /gene="rnhC"
                     /locus_tag="ACHGNS_000168"
     CDS             complement(57769..58686)
                     /gene="rnhC"
                     /locus_tag="ACHGNS_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFN6936152.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            58888..59325
                     /gene="zapA"
                     /locus_tag="ACHGNS_000169"
     CDS             58888..59325
                     /gene="zapA"
                     /locus_tag="ACHGNS_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFN6936153.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            59383..59931
                     /locus_tag="ACHGNS_000170"
     CDS             59383..59931
                     /locus_tag="ACHGNS_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFN6936154.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            60046..62406
                     /locus_tag="ACHGNS_000171"
     CDS             60046..62406
                     /locus_tag="ACHGNS_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFN6936155.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            62493..62807
                     /gene="trxA"
                     /locus_tag="ACHGNS_000172"
     CDS             62493..62807
                     /gene="trxA"
                     /locus_tag="ACHGNS_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFN6936156.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            62989..64767
                     /gene="uvrC"
                     /locus_tag="ACHGNS_000173"
     CDS             62989..64767
                     /gene="uvrC"
                     /locus_tag="ACHGNS_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFN6936157.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            64912..65811
                     /locus_tag="ACHGNS_000174"
     CDS             64912..65811
                     /locus_tag="ACHGNS_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6936158.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            65804..67066
                     /locus_tag="ACHGNS_000175"
     CDS             65804..67066
                     /locus_tag="ACHGNS_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6936159.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            67227..69125
                     /locus_tag="ACHGNS_000176"
     CDS             67227..69125
                     /locus_tag="ACHGNS_000176"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFN6936160.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(69455..70174)
                     /locus_tag="ACHGNS_000177"
     CDS             complement(69455..70174)
                     /locus_tag="ACHGNS_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFN6936161.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    70364..70411
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            70511..70843
                     /locus_tag="ACHGNS_000178"
     CDS             70511..70843
                     /locus_tag="ACHGNS_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936162.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            70830..72833
                     /locus_tag="ACHGNS_000179"
     CDS             70830..72833
                     /locus_tag="ACHGNS_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFN6936163.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            72852..73322
                     /locus_tag="ACHGNS_000180"
     CDS             72852..73322
                     /locus_tag="ACHGNS_000180"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFN6936164.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            73343..73927
                     /locus_tag="ACHGNS_000181"
     CDS             73343..73927
                     /locus_tag="ACHGNS_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFN6936165.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            73942..74928
                     /locus_tag="ACHGNS_000182"
     CDS             73942..74928
                     /locus_tag="ACHGNS_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFN6936166.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            74918..75229
                     /locus_tag="ACHGNS_000183"
     CDS             74918..75229
                     /locus_tag="ACHGNS_000183"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFN6936167.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            75251..77032
                     /locus_tag="ACHGNS_000184"
     CDS             75251..77032
                     /locus_tag="ACHGNS_000184"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFN6936168.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            77025..78401
                     /locus_tag="ACHGNS_000185"
     CDS             77025..78401
                     /locus_tag="ACHGNS_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFN6936169.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            78408..79043
                     /locus_tag="ACHGNS_000186"
     CDS             78408..79043
                     /locus_tag="ACHGNS_000186"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFN6936170.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            79073..80428
                     /locus_tag="ACHGNS_000187"
     CDS             79073..80428
                     /locus_tag="ACHGNS_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFN6936171.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(80620..82014)
                     /locus_tag="ACHGNS_000188"
     CDS             complement(80620..82014)
                     /locus_tag="ACHGNS_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFN6936172.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            82106..83077
                     /locus_tag="ACHGNS_000189"
     CDS             82106..83077
                     /locus_tag="ACHGNS_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFN6936173.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            83074..83856
                     /locus_tag="ACHGNS_000190"
     CDS             83074..83856
                     /locus_tag="ACHGNS_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFN6936174.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(84025..84396)
                     /locus_tag="ACHGNS_000191"
     CDS             complement(84025..84396)
                     /locus_tag="ACHGNS_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN6936175.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            84530..84973
                     /locus_tag="ACHGNS_000192"
     CDS             84530..84973
                     /locus_tag="ACHGNS_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFN6936176.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(85016..85825)
                     /gene="yidA"
                     /locus_tag="ACHGNS_000193"
     CDS             complement(85016..85825)
                     /gene="yidA"
                     /locus_tag="ACHGNS_000193"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFN6936177.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(85844..87211)
                     /locus_tag="ACHGNS_000194"
     CDS             complement(85844..87211)
                     /locus_tag="ACHGNS_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFN6936178.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(87195..88025)
                     /locus_tag="ACHGNS_000195"
     CDS             complement(87195..88025)
                     /locus_tag="ACHGNS_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFN6936179.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
BASE COUNT        28969 a        14921 c        18109 g        26045 t
ORIGIN      
        1 tgttaaataa ataggaacaa aaaaattaaa ttatttaata cacacattag ggatcattca
       61 ttgaaggatt agaagacaac gaggatggcg aaaaatatga ttacaataga aaagtcgttc
      121 taaaccaatc agttcctagt atcacacaac gcaagccagt cgatttggtg agattagagg
      181 agtgaaagca aactttatta tataagaaaa aatattcgaa agggatgcta gtttaggtac
      241 tagtgctgat agattaatat gatgcctcgt aatattaggc gtagttactc tagagacatc
      301 actatggact atgatttgtc tcagagtcac aacatgtttt tttatgaaaa gaaacagcta
      361 aaataattaa attgcgcaat gtttaaactt tcttaaacta taacctaata aatgtgaaga
      421 aataatgcac aatttcttca catttatctt ctattctata tacataccaa ccaacaaccc
      481 tatatacttt ttcattttta ctcctcaagt aaaaataact cctagaccgc ggctacccac
      541 cgcggtcgtt tttttattct tcaatcaaaa tcgctttgat acccaaacct gccaagattc
      601 gggcagaagt ataagtattc aatcctcttc tgaaagtaat gtaaactttt ttatcttttg
      661 ggatttcatt gattcgatct cgtaactcat tcattggaat attttttgtt gccgagatac
      721 tgccagaagg tgctttatta ggttcacgta tatctaaaaa gaacgcttct ttcaaatcgt
      781 tttcgggtat atcacttgct ttgatcgttt ctacgatatt cgtcatttga ttgattgcga
      841 cataaccagc tatattcaac gggtcacggg tagtagaata cggtggagag taaggcaact
      901 ccaaatcagg caaatcaaag actgtcaagt ttccggtgat tgctacagaa agttctccca
      961 tccgtttatc gacacctttt tctccaaatg cttgtccacc taaaatacga ccgctatctg
     1021 catcaaaaat cagctttaga tttaatcttg tagcttctgg atagaaatag gcatggtcaa
     1081 agggagtgat aaaaacagtt ttataatttg ttatgcctga tgcttttaat tcatgttctg
     1141 tcattcccgc ataacttgct gtatgatcaa agatttttgc gacacctgca ccaatatatc
     1201 cgcgatagcg cataggcgta ccatttaaaa tatctgccag catatgtcct tgacgattgg
     1261 ccgcactgga aagcatactt tggatcggct gaccagtcac tacactagtc gtttcgataa
     1321 tatcgccaat cgcatagatg tccggcagat tggtttgtaa ctggtcatca acaatgatct
     1381 gacctgtatc tgataattgt atgccggttg ctttcaccac ttcattcttc ggagaaacac
     1441 caacagcaaa aattagcata tcagcagaaa ggacggagcc atctgataat actatttctc
     1501 gacctttatc acggatctca gtaactcttg tttctaaatg aacggctaat ccttctttca
     1561 gcaatttgtc ataaactaaa ttagcaattt ctggatcata agggaaagcg acttgtggca
     1621 attgatcgat taaagtcacg tctaagccgc gatgcttgaa attctcagct aattccagac
     1681 ccataacacc tgcaccaaga atgacgacat gttgtggatt tttttcttca aggaacgatt
     1741 tgattcgatc cgcatccgtc acactacgga ggacaaagcc gttttctgct tcaaaaactc
     1801 cgggaatatc cggataatct ggtctagcac cagaagagat gatcaactta tcgtaatctg
     1861 tttttatttc ttcatttgtg gataggtctt taactgttaa tcgcttagta gaaggatcga
     1921 ttgctgttac ttcgtgttta gtaaagacat cgatgttgtt ttttgttttc aatatttctg
     1981 gtgtacgttc gatcagcgag tcacggtcag tgatcacacc tcctaaataa taaggcaaag
     2041 cacaacttgc ataagaaata ttttctccgc gttcatagat aatgatttcg tgcgcttcat
     2101 tcaatcgacg gaaacgagtg gcaaatgaag gaccgccagc tacaccgcca atgacaacaa
     2161 ctttcataaa aacatctcct tttctacgtt gatcgtacta tgttatggta aaaaacagca
     2221 aataattgtg ccggaaaatt attgtttatt ttgaatagac agaaaatata gagaaataat
     2281 ttgtggataa aacctaaaaa tttttttcta ttttaaggat agataatact ttgaagggga
     2341 taaccctgta tattttttaa aagctgaaga aaaatgatgg atcgaatgaa atcctaatat
     2401 atctgcaatt tctgtatagg ttaattcatt ttctagcacg agacgttttg ctttttccat
     2461 ttttagttct ttaaaatagc taataacact ttttccattc acttttctaa aatgtttttt
     2521 taagctggct ggactgataa aaaaaatttg ggctaattgt tcaagcaaaa aattttctga
     2581 aagatgttct tctaaatatc gtatgattgg atcaactaat tcatctgaat aaagaatatg
     2641 ctttttttta ggaatgactt tctcatttga aggtattgtc aataaagcaa gttcctgttg
     2701 ataatgcctt ggtaaggaag acaattgaat tttttgcaga caataagtaa ctaataagga
     2761 aagcaaatga atgctcatct gttggcatag tatgaaaaaa gaattgattt cctgatcata
     2821 ggaaatagct ggcttactat gttttagata atcaattttg aactgttcat ttttcagaaa
     2881 agtaatgata gatgttattt cttgatctat agaaaaagat tgtcccgtaa tatgtgctaa
     2941 taattgctca tcaaaataaa actggataat gataaaagtt tgtgtttcct tttgagtaga
     3001 aaagatttgg acttcttgct gtttatcaat gaataaaagg gaaggattat ctagttctat
     3061 agatttatgt gcaaattcag catataaatt agcgctttca tttattagta gtcgaaaata
     3121 gggagcagca gataaatgaa gatgggtatc tatggaatag gttagtgttt ttttgacaag
     3181 attggaggat tggttgtttg ttaacaaaag aaccactttc ctttctcttt ttatataaaa
     3241 agctttctat ttataattaa tgactattat aacaaaaatg aaaagccaaa acgacaagta
     3301 aaatattttt ttatataaat atttgtgtta ctaattccgt taatatacat aagtgaaagc
     3361 gttttatata agaacttgaa aagaaagaag ggatagacat gaaaaaaaga tacaaattgg
     3421 tgatagcttt gctaaacatt accctattat tcagcttaag tgcatgtgga aaaactaacg
     3481 aagaagtaac agcaaacatc gcagatccaa tgataaaaaa agccactaca ttagaagtat
     3541 atgtcgcaaa cggcaaactg acaaactatg ttgaaaatgc tgcaaaaatc tataaccgca
     3601 aaactggagc aaatattgac ttaaaaattg tgaatgtagc ttcgggaaca gcaacagtac
     3661 aaatgatcac cccaaaattg gtaacacacg aagatatgcc ggatattatc tccatggcag
     3721 acacttcagc agcaggtgtg ctggaaaaat ttcaagatgc tttttatagt gctgatgaat
     3781 atggatttta tgacaaatat ggcgatcagt tttatgatca aaaattgaat attttgcgtc
     3841 aacaaacgag taatgaaacg attattcctt gggctagtga ttttacccca gcactttgtt
     3901 attatcaacc aaaaaaattt gctgaaatag gtgtagattt tgaagaaatc agttcttggg
     3961 aagaatatat ccaggtggca aaagcattaa aagaaaaaac tggtagtttt ggaattgcct
     4021 tgccagaatc tggggaccaa gaattgttta tcaacatgct agcccagcag aatcaatctt
     4081 tgcttgatga agaaggaaat atccgtctga atactaaaga agcgaaacat gctgcaaagt
     4141 taatcaaaga aatgatcgac tcagatattg tacatttcta cggtgcacaa gatgctgaga
     4201 aagcatttca agaatctgca atgtttgttg ctggcggctg gtatgctacg aatatgtctt
     4261 tgaattttcc tgatgcatct gacaaatggc gcatggctcc actgattcct ttttcaaaag
     4321 aaaatccagg aaaaggtgca gtatcaggag gatcaagttt ttatgtccct aaagatgcaa
     4381 aaaatccaca agtggcacaa caatttctta catttatgct gacagatgaa gaatgcttag
     4441 ccaacgcact ggagctgggc gtggctacat ctaactcatt ggcctatcaa acagaagcag
     4501 ctgagaaaaa gtttgactac taaggaggac aaaaatacta tgaactattg aataaaatca
     4561 atgaaaatac atcaaatact gttttaccag ccacttattc agatgctacg tcttatttga
     4621 ctaccgctgc atataactat tggcaaacag gtgattttga aaattcatat ctaaaagaag
     4681 cagaaaattt tgctcataag tacggcgtaa aattaactgg tgattaaaaa atattaggag
     4741 gacttaacga tgatgcaatc ttcgttacca agcaagacaa taaagaacag caagaaaaaa
     4801 gcaacagata cgtctcataa aaaaagtgct tatggattgg tcacaccttt tgttattttc
     4861 tttattgtga cgattttgat tcccatcatt atctcgtttg cttttagttt tatgaaaatt
     4921 ggctactcat ttgaatttgt tggatttgac aattatctag ctatcttccg agatccattg
     4981 tttattaaat cttacacgaa tgttttgatt atcatggcag gatcaattcc aataacgatg
     5041 ctattagcta tttactttgc tgttttattg aattcagcac agttaaaagg aaaaggattt
     5101 tttagaactg tctattatat tccaaccatt acatcaacgg tggctgtagc ttccgtattc
     5161 atgacttttt tcaatccgac tgggttattc aatggatttt tgaatgcgct aggaattaac
     5221 ggtgtacagt ggttaacaaa tcctttttgg atacgaatat caatgatcat cacgatgatt
     5281 tggcttagtg taggatataa cactatttta tttttagctg gtcttcaagg gatttctaaa
     5341 gagatatacg aaagtgctga aatagatgga gcatcgaaat ttcggcaatt ctttttatta
     5401 acgatcccca tgctaaagcc aatcatttta atggccacag ttttagccac tatcaatggc
     5461 ttgggtaatt tcaatatccc aaatatattc tttgggactt ccaatggacc agaaaattca
     5521 gctttggtcg taggagtcaa tttatataaa acaagttttg aaatggttga ttttggaaaa
     5581 gcctctgcta ttgcctggac gatggtgttt gtatctgtta tattatcggc tattcaattt
     5641 aaattcggag gtgataaaaa tgactaaaca aaagatacaa agcagaattt ttacgtacgt
     5701 cattattgtg tttggtgcct taatattttt gatgccatat ttatatatga ttgtagcttc
     5761 aacgcaaagt aatcaagaaa ttttaagtgg cggaatcaat ttcaagtttg gggatatgct
     5821 attaaaaaac tggcaagcca tcatggcgag atataattat cccttgatta tttggaattc
     5881 gattgttatt gctgtaatca gtacagtttt agctacagcc agttctacaa tggcgggtta
     5941 tgcattagca aagtatcgtt ttatggggaa taaactgttg ttcaatacag ttatgctatc
     6001 taggatggtg ccttttttta ccacattgat tcctttattt tacattatga gtcgtttagg
     6061 attatcgaat acttatgcag gtgtgatcat cccttcgatt gccagtacaa cttcagtgtt
     6121 tatgatgcga caatatagca gtcaatttcc tacagcactg atggaggctg ccagaatcga
     6181 tggtgccaat gagtgggtga tttttacgaa aatcgctgta ccgattttat cacctacgat
     6241 tattactagt ggtttactta tctttatggg aagttggaat caatatctat ttccattagt
     6301 catgctttca gataaagata aatatacggt tcccttaatt atcaaaaata tgacggtttc
     6361 tagtacaggt gagccaatta attatggcgc aattatgcta attttagcaa cttcagtgat
     6421 cccaatggtc ttgatttatg tgtgggcgca gtctaaattt aaggagaatg acattggagc
     6481 tgcaaataag taaaaaaaat aacgaatgtt aaccatctag ttcgttctag gagcaactcc
     6541 acattcataa aaattaaaag aatggcggga tatccatgaa tgaacagacg aaggaatggt
     6601 caagaaaaat tttgaatgta gcttctgaaa agatagatgc ggaattagac agaatgggga
     6661 ataaaattcc atatatttct tacaacggaa aatatacaga cgtaataaag gacagaggaa
     6721 tcgactggtg gaccaatggt ttttggagtg gtattttatg gcagctcttt cacaagacaa
     6781 aacgagaaaa gttcaaagag gcagcaatac aacaagaaca attattagaa tctgccctgt
     6841 accatttttc agacgttcat catgatgtag gttttttatt cttaccatcg gctattgcac
     6901 attatcaaca gtctcaaaac aaacatagtc gacaactagg gttacatgca gctacggtac
     6961 ttgctggacg ctataatatt tcagggaact atttagtagc atggaatgac cataaacctg
     7021 gttggatgat tattgatagt atgatgaatt taccattact gtattgggct tcgagggaaa
     7081 ctggtgatcc gaggttctct aaaattgctc ggcagcatgc agatacaaca gctaagtatc
     7141 tagtgaggaa agatggatca accgggcata tcgcagaatt tgatgcagaa tcagggaaat
     7201 atattcggtt acttggcggg caaggataca atgagaattc ttgttggagc agaggaaatg
     7261 catgggcttt atatggattc gcgttaagtt atcgttacac aaaaaatccg tactatctag
     7321 ctatagcaga aaagatagca gtaaatttta ttcagaaaat cgaaaaaact ggttatattc
     7381 ctctcattga ttttgctgta aaagattcga atattgatac cagtgctgga gtatgcgctg
     7441 catgcggtct tctggaatta gcaggatatg tttcctctgt acgatcaaaa atgtatcatg
     7501 aagctgctat caatatactc caaaagacag aaaagcacta tgcaaattgg aatatggatg
     7561 aagatgggat cattggaggc ggagcagaag cataccatag agaactgact tataatgtgc
     7621 cactgatcta ttctgattat ttttttattg aaggcttact tcgattagaa cagtcggaat
     7681 tagctatttg gtagtaaaaa gaggagaaaa tacaatgaaa catccagtac cactattaag
     7741 gaccagacag gattactttg acttatttaa aaagataaat caaccactcc agccgtttta
     7801 tcgagcaagt ggcgcacaaa ttgattatat acatcaaggg gtaggatatg gaaataaaat
     7861 tgcaggaatg gaaggattct ctcgggtttt atggggagcg gggcccgcaa ttgaccaatt
     7921 agatgaacga tggttgaaat taattctatc aggaataaaa gctggaacag atccttccca
     7981 tcccgattat tggggagaaa tacatgaaag agaccagcgt atggtagaaa tgcccgcaat
     8041 gttattagcg ttatatcatc atgaacaatt attatggaaa aaattagata caggagaaaa
     8101 aagccagatt attcattggt tctcgcaaat ttttgaatat gaatgtgcag atggtaattg
     8161 gcaatttttt aagataattg tcggaacaat tatccaaaaa tttggttatc cagttaaaga
     8221 atcagcactg aaagaagcat ttgataatat agaaaattgt tatcttcaaa atggctggta
     8281 tcgagacagt tcaagaggtc gccaagatta ttataatcct tttgcatttc attattatgg
     8341 tttgatttat agcgtactag aaccagaaaa gcccattagc cagatatata agaaccgagc
     8401 tattgatttt tctagagact atattcattt ttttgcggaa gatggtgcaa atgtgccttt
     8461 tggtcgaagc atgatttatc gttttgcagt ttcaagcttt tggtcagcta tgttgtgggc
     8521 cgatcttttg ccttttaaat taggtgaaat caaggggtta gtcaatcgga atataagatg
     8581 gtggatgcaa aaagaaatat ttgatgaaaa aggtatcctg aatattggtt atacctaccc
     8641 tcaattgaca ttgactgaac cctataatag tacgttatct ccattatggc tgaataagat
     8701 ttttttattg ttggcgctac ctgaagacca tgagtattgg acggctaaag aaatgcctat
     8761 gcctattcgt gaaggaacta aacttctttc agaagccaat ttgctcatac aacatgataa
     8821 tggacataca ttttttttga atgcgggaca attagggcca aatttccata cattgaccaa
     8881 tgaaaaatat ttaaaatttg cttattctag tcaatttggt ttttcgatac cgcgagcgaa
     8941 tcaattgaaa gcagaaatgg caatggattc aatgctaggg attcagcgag cggacacata
     9001 tatcacgacc agttttaaga atgagagcat caaaacctat ggacagttca tcgttcgaaa
     9061 taatgtacga gacattgtat gtaagaaaga ggtacttgcc tctacatggg aacctacttt
     9121 acaggcaaaa atcagaactt ggcttattcc ttttgaaggc tggcagattc gcgtacacaa
     9181 agtagaatta gatacagaat atgttctata tgagacaggg tttgctattg aatgttctcc
     9241 tgagaaagaa ggaataatca tagaagaaga caaggaaaac tatagggtga gtgaagctgg
     9301 cttttcaggt attatctgtt taagtgacga tactattaaa cgaaaatcca cagctattat
     9361 gggtttgccc aatacaaact tgatgacatg ggaaaacact tttattcctg gattagaggg
     9421 tacatttgga aaaggaaccc attggctcgg aacaggagtg gttgctcatc aagatcggga
     9481 ttatagttta gaagtatgga acaatcgtcc caaaattgat tttgatggga caaccgggct
     9541 aactatccaa aataaaaaca ataaaaaaag actaaaatta tgctaaaggc tggtggggaa
     9601 atgcttgata aagtgatgtt tgaagagata atacatccag aaaaatggat aaatgaatgt
     9661 tataaagaat taatggaaaa agaatggaat aatgagatcg agcgttgtcg cagacacacg
     9721 gagaggttaa tcaaacgtat cagtaacagt cagtatcaat tcccaagcga gtgtcctata
     9781 aaaggacgtt atccattaag agacaatatt ggttggacag aaggattttg gacaggaatt
     9841 aattggttgt tatttgagaa gacgggtgat cctttatttc aacaaacagc tgaaaaagca
     9901 gatatagatt ttatccacag gattgactgt caaattatca ctaatcatca cgatcttggt
     9961 tttttgtatt ctttatcaac cgtagcagat tataaaataa cgggaaatcg aaaaatgaag
    10021 gaagcttcaa ttgatgctgc agaaacatta cttcagcgtt tttgggagaa ggcaggagtt
    10081 atacaagctt ggggagatcc tagtgatcct tcacaacaag gtagaatgat catggattgt
    10141 tgcatgaatc ttccattatt gtattgggcg agtgaagaaa ctggaaatca aaaatataaa
    10201 aaagctgctc aggctcatat tcaaaatgca gcagattatt tagtaagaga agatggttca
    10261 acttatcata cattttatat ggatgtcaaa gatggaaaac caagatatgg aagtacgcat
    10321 caaggttata gtgataattc ctgttgggct agaggccaag catgggggat ttacggtttt
    10381 gcattaagtt atcgttattt gaaagaacaa aaatattttg atttaagcaa aatattagcc
    10441 aattatttta tgaatcggtt gccagaagat accatttgtt attgggattt gatctttgat
    10501 gattctagca gagaagaaaa agatacatct agtatggtga tcatggtttg tggattatta
    10561 gaattaatag aatcaggtct attagacaaa acagaaaaga acttatacca gtctgctgtt
    10621 tggaaaatta tgcgacaatt gaaagaaaga tatgctacaa cggaagaaga agatggcttt
    10681 ttaacacagt ctgtttataa taagcccaaa aataatgggg taaatacacc ttgtatttgg
    10741 ggtgattact attatttaga agctttagtg agaatgacta gtaattggtc ttcttattgg
    10801 tgaaaaaggt aaatatggaa tttttgtttg ataaaataaa aaaagaaaaa ccaaaacagc
    10861 atgaatggtt tctgttttgg ttttttcgtt aattttcata ttcttctaat tccaagctta
    10921 gttcttccca ttccgtcaat tttgtttctt gatcttgtct agcagcttct aaatcttggt
    10981 tcagctcatt gagttttacg tgatcttcca ggttttctgg cgcgaccatt tcttgttcca
    11041 actgatcgat ccattcatcg atccgcgcca tttcttcttc aacctgagta atttttcgtt
    11101 gaaggctacg gataagcttt tgctgttctt tgctttggta ataatcagat ttgttcgtgg
    11161 gagcaggagc agattctggc tcatcttgtg ccaacaattc ggcgatttct tcttcttcct
    11221 gctttttctc taaataataa tcatagtctc ctagatagac tttgctgcca ttttcagaga
    11281 gttcgacgac ttttgtcgcg atccgattga tgaaatatcg gtcatgggaa acaaaaagca
    11341 gtgtgccgtc ataatcgatc aatgcatttt ctaatacttc tttgttgtca atatctaaat
    11401 ggtttgttgg ttcatccaaa atcaaaaaat tttctttgtt catcgctaac ttggctaaag
    11461 caacccgtgc tttttcaccg ccacttagca gagggattgg tttttctaca tcttcgccag
    11521 aaaataagaa agcacctaaa gcacttcgga tctctttttc tggagtggta ggatgctcat
    11581 cccacaattc tgctaggatc gtcttgtttg tatgcagatc agcttgacct tgatcgtaat
    11641 agccgatggt gacatttgtc cctagcgttt cttttccacg gataaaaggg atctggtgaa
    11701 tgattgattt cagcaacgtc gatttcccga taccattcgg acctactaga gcaatcgctt
    11761 cttgacgacg gatatctaga ttgattggtt cagataaggt gacattatca tatccgatag
    11821 cggcatcttc gatttgcaag actacatttc cagaaggctt ttctatactg aatagaaagt
    11881 ttgcttgttt ttcatcccct tgaggacgat ccagccggtc cattttttct aatgtctttc
    11941 tgcggctttg cgctcgtttt gtggtagagg cgcgaacaag gttccgagca acgaagtctt
    12001 ctaatttatg gatttctgtt tgctgctttt cataagcctt ccattcactt gctaactgtt
    12061 ctgctttcaa atctagatat ttcgaatagt tacctttgta atggcgcatt tttcttctgc
    12121 ttaattcata gacttcattg actactttat ctaaaaaata acggtcatgg gaaacaatca
    12181 ataatgcacc agtatagctt tgcagataat tctcaagcca ggaaagtgtc tcgatatcta
    12241 aatggttcgt tggttcatct agaatcaaaa tgtcagggtg ttgcaaaagc atgcgagcta
    12301 aagctaaacg tgtgcgctgt ccgcctgata aagtatcgat tttctgatca taaaatgaag
    12361 aatcgaattt aaacccatgc aatacggaac gaatttcatt ttcataacca tagccgtttc
    12421 gatctgaaaa atcgtgttgc agccggtcgt attcttttaa ataagaacta taagcaggtg
    12481 tgtctggcgt accttcgcta atgaaaatct ctagctcgcg catccgctct tccatttgtc
    12541 taacttgttc aaaagctttc agcatctcgt tccaaactgt ttcattggaa tcaagtcctg
    12601 tgtcctgagc gaggtatccg agagtcgctg ttttattttt agcgatcttt ccttcatcag
    12661 gtgcttcaat tccagcaatg atctttaata aagtcgattt tccagcgcca tttctgccaa
    12721 ctaaagcgac acgagcgcct gtactgattt ctaaatgtat gttttcaaat aagacttctg
    12781 caccaaaatg gcgagcaatc tgattggctt gtaataatat catggaatcc tcttttctac
    12841 tttgtgaatt ctcctcaaat aaaaaactat ttttcaatta gtagtctacc ataaattcca
    12901 ttatactgcg agatttgcag aaatgataaa taaatgaata aagaataaaa tatcacaata
    12961 tctgagcaat ggaattgatt ttttcctata aatactgcta tgatggagaa atcaaagatg
    13021 taattagaca atacgtggag gtcatttcgt gaaagatcat attattccaa aggcgacagc
    13081 taaaagactg cctttgtatt atcgctattt aaggatttta aataatgctg gaaagaaaaa
    13141 agtgtcttca actgaattaa gtgaagccgt tcaagtagat agtgcaacga tccgtcgaga
    13201 tttttcttat tttggcgaat taggaaaacg tgggtatggt tatgatgtag aagatcttat
    13261 gcactttttt gcaaagacat taaacgacga tcaattgaca aatgttgcgc tgatcggtgt
    13321 agggaacttg ggaagtgctt tactaaaata caaattccat caaagtaata gcatacgtat
    13381 cagttgtgct tttgatgtga atgaggaaat cgttgggcgc atcgtagacg gtatccccgt
    13441 ttatccaatg gaagatatgt cagaacaaat caagatccaa caaatcgaag tagcaatttt
    13501 aacgataccc gctgaaagag cacaagaagt agtcaatcag ttaacggaag caggaatcaa
    13561 aggaatcttg aactttaccg ctgctcgatt gaatgctccg tcagatgtat tgatccaaaa
    13621 tgttgatttg acgaatgaat tgcaaacctt gatctatttc ttgcattctg acgcaaatgc
    13681 gcattccgaa aatacagcag aataaaaata aaaagcgatt gaacaaatga aggcaaaact
    13741 ttcacttgtt caatcgcttt tttctgtttg gacaacgatt attttgttcg gaagatttct
    13801 tcataagaaa gggaatcatc ttctgctgtc agttcatcta gtagataacg gattttttta
    13861 aagtcgatga accgtgcttg ccgaaagact tcttttcctt gatgaaaaat caggacaact
    13921 ggcgcagtca gcacttcaaa tttactagct acttctggtg tttcaaatgc atccaaatga
    13981 agtgccggaa tatcataatg tgtgagaagt tcctctagtc gtggacgaac agcgtgacaa
    14041 acactgcaat taggcatcga aagataaatg acggcaacgg atgactgttg cagtatgtct
    14101 tgaacttcag tcaagttagt ggtttgtttc atttttattc tcccttttat gatagaagtg
    14161 atatgtatga gaccttaatt taatgggaat aacagaaaaa gtcaattgaa gtgctttggt
    14221 aaaaaataag agttgtgtaa aaatttacag agcaacttgt tttttttgaa taaaagcgta
    14281 tatttgcaaa gcgaatgaac tagaaataaa aaggaggaaa ctcatgtttt cttttattcc
    14341 aaattcggtt ttgcagatga gtacgatttt cttatcgatc gttattgaag cattaccttt
    14401 cgtattatta ggctgtctta tctcaggcgc cttacaagta tttttgaccc cagaacgtgt
    14461 gaagagatgg cttcctgaaa atcgtatctt ctctattttt actggttcga tcttaggatt
    14521 tttctttcct tcatgtgaat gcggcatcgt accgatcgtt catcaatttg ttaaaaaagg
    14581 cgtaccagtt cacacagcgt ttgcttttat gctgactgct ccgatcatca atccaatcgt
    14641 gattttttcc acttttatcg catttggcaa ttcttggcag atggcaagct ggcgggtgat
    14701 aggcagcttt tttgttgccc ttgtgattgg tgtctggctt gcctattttc aaaaagacgc
    14761 tgtattgaaa gaaaaaatca cgtctaaagt agcagaaaat caccatcatc atgtccatga
    14821 agaagaacaa agtgagaaga atgaaccaaa aaatcagctg atccatcaaa ctgggcatgt
    14881 gttgacacat tcaatcgacg aattttttga tacaggtcgt tatttgatca ttggcggatt
    14941 aatcgcagct gcgatgcaga cttatttacc aactagaatt atgctaacgc tcggctcaac
    15001 aaagatttta gccattatca tcatgctgtt attggccttt acgatgtctc tttgctcaga
    15061 agcagatgcg tttataggat cttctttgtt aagtctgttc ggcacagcac ctgtagctgc
    15121 ctttttggtc tttggcccaa tggtcgatat caaaaatcta ctgatgatga aacgatattt
    15181 tacaggacgc tttattttat cgctgatcgg actgattgcc cttagcgtta ttggattcac
    15241 attatttatt taggagggaa aatatgctgc ggtttttgat tttatctggg tatttcgaat
    15301 tgatgatgta cttgcaagtt tctggcaaac tcgatcaata tatcaatgtc cattatcgct
    15361 acttagctat tctttcaatg attttatctg ctcttttggc tttagttcag ctgtatttgt
    15421 gggtcaaaaa cggaaaagca gaagaaaagg cacacgagca cgatcatgac catgggttgt
    15481 caaaacctta tcaacgtgga atcgcttatg tactgctagc tttgccagta atcgtgggga
    15541 cgttgtttcc aacagttagt ctcgacacta cgatcgtaga agccaaagga tttaatttcc
    15601 cagtaagtaa agaatcagta ggggatccgg agatgcagac acaatactta aagccggata
    15661 ccagtattta tttcaacaaa tcagactact tagaccaaat gaatgagttg aaagaaaagt
    15721 atggggataa gcaaactatc aagatcacag atgaaaacta tttagaagtt atggagctaa
    15781 tttataatta ccctagcgaa ttcattggca aaaaaatcag ctatgaagga tttgtttacc
    15841 aaacaccgaa cgatagcaat acagatatct tcttgttccg attcggcatt atccattgtg
    15901 tggcagattc aggagtcttt ggtttgttga cgcatatgcc ggaaggagta acggttaaaa
    15961 atgatgaatg gtataaaata gaaggaacca tccaatcaga ctattatcaa ccattcaagc
    16021 gtgaaatccc atctgtagtt gttacaaacg ctgagaaagt ctctgctccg aaaaatcagt
    16081 atgtgtatcg ttctttttaa aagaacaaaa taaataaaca tagctcagac agctgacacg
    16141 caaggacgtc ggttgtctgg ctttttttgc atgatgtatt catttgtttg atatttattt
    16201 tagaagcagc atcctgaaag tatttgatat cggtttcaaa ggtgttggaa aagattgtga
    16261 agagaattac aatttattcc taacatgtat gttgaaaagt tgtatgaaat gattttgata
    16321 gtaaaatatc gagtggaggt actttttatg gtaacgcgta tatttatttt ggatgatagt
    16381 ttggtttttg aaaaaatgct agtagaaatt ttggaagaat ccagaatttt attaagtcct
    16441 tggaaatttg agatcgttca gggattgaac gttatacaat ttgttgaatt cattaaaaat
    16501 aatgaaatct attcctcaga tatttttttc ctagacatcg atttgaacgc ctattatagc
    16561 ggtatcgaac taggcgaaat gcttagagaa aagaatgaaa agtgtaaaat tgtttatcta
    16621 acgtctcatc cgaataaagc aataactgtt attaatcgaa agattacgcc tagtggttat
    16681 ttgataaaag aattcactta tgaaaaaaat aaaaaaagca tccaaagtta cttattgaaa
    16741 gaaaaagaaa actatcctgt tcaacatgct acgaaaaaaa ggtggacaac tgtccgttat
    16801 ggtaatagcg atcactatat ccattacgat gaaatccttt atctaatgag catgccaggt
    16861 tataaaaata aaataggcct agtattgaaa gatgaagaga ttctcgtaaa cggcacaatt
    16921 aaaaaatata aaaaatcact cgaatatgag tatttatgta aagatttaaa agcttatatc
    16981 attaatactg aaaacattac atctattagc agagtaaacg aaatagtaac atttgcaaat
    17041 ggagaacaac tagagatggg aaagagaagt attgataaac taaagaattt tatgcaagaa
    17101 aagatagaaa atgaatcatt ttaaagaaaa caaagactca tatcttacct gttagaagag
    17161 tgttttcctg tagaagagaa caacccaagt agcagcctag tcgtttctat ccttttttgt
    17221 tatgcactct ttttgacatt tactccacca ataacgaagg gtacaataaa aagtaataac
    17281 aaacaaaagg agtacagaaa tgattgaatt catcaatgta agtaaaagtt acgggaaaaa
    17341 acaagcatta aaagacttgt ctctctcgat aagacaaggg gaaatttttg gatttttagg
    17401 acataatggt gccggaaaat caactacgat taaaagttta gttagtatta ttgaaccatc
    17461 atcgggaacg attttagtag atggaatgaa attgacggaa aatcgattga gtatcaaaca
    17521 aaaaatcggt tatgtgcctg attccccaga tattttttta caattgacgg ctggagaata
    17581 ttgggacttg atttcagctg cctatgaatt agacacacaa aaaaaagaaa agcgtttagc
    17641 agaattgaca gcgctttttg atatgtatag tcatcaaaat gaaacaatcg caagtttttc
    17701 acatggaatg cgtcaaaaaa cgattttgat cggcacgttg ctgccagatc cagatatttg
    17761 ggtattggat gaacctttgc aaggacttga tcctcaagca gcatttgatt tgaaagaaat
    17821 gatgaaggct catgccgcaa aaggaaaaac agtcattttt tctacacatg tattggatac
    17881 cgcacaacag ttatgcgata aactggctat tttgaagaaa ggtgaattga tttatcaggg
    17941 ggcagttagt gaattattga atggctcacc tgatgaaacg ctagaaaaga tttatttgaa
    18001 aatggctgga aggcaagcga caggagatgg accgtatgta taagcgacaa ttattagaat
    18061 taattcgagt taatctttta tatgttaatc cacagctcac gaataaagca agagagaaag
    18121 gaaaaaaggg aaagcgatta actatctatc ttttgtctca aaatcttttt tcagggctga
    18181 tttttttact gatttacgga tttgctatgt ttgctatgaa ctttgttaaa ttgccaggtt
    18241 tctttactta ttacgtggca ctttttggta tgttaggttt aactcaaagt atatcagtca
    18301 tttataatat cttttttgaa ggaaatgacc tgcaagtatt tttgcctctg ccttttggac
    18361 aagaacaggt tttttcagca aagattttgg tagttgcttt gaccatcatt ccttttgttt
    18421 ttccgcttct agtcatgttt attttaaccg gatggcgagc tgatctaggg attttgctta
    18481 ctatcattat ctcaatcctt ttgttttctc tatttttgat tattcttttt tgtctggcta
    18541 gtttgattgt ttttggtttg gctcgaacag cagtgtttaa aaaacataga aaaacagttt
    18601 ctagtatctt gcttggcagc tcgatgctga tcgcatttgc tggaatcatg tggatgaatc
    18661 atcaaacggg gactattgaa aatgagttaa cagatcggca tccgatttcg atattattgc
    18721 cattctatga tgctgccagt cagccacttt ctcaacaagg tttattgggt tggggaatac
    18781 tagctatcac ggctctggga tttttccttg ctgtaaaaca gctgatagta ccaaagctta
    18841 cagagcaact gactgattca aacagtgagt tcaaaataaa aagaacccat aaaaagaacc
    18901 aaaatgtgca tcagctttta tttagttaca atgctcaatt agtaaaagaa cctaatttga
    18961 ttatgcaagt tctttcttct tccttgttaa ctccaatcat ttttattctt gcttttgccc
    19021 taggaggcga aataaaatta actgatttag ataataagtt aatcggtgtt gttttcctgg
    19081 tagggatcgc attggctttc ttaacagtta atcagacttc ttttatcagc aatcttattt
    19141 ctttggatca agaaaatttt ctgtttatcc gttctttgcc aatctctatg aaccaatatt
    19201 taaaagaaaa gttcagattc ggatggatat tgcagagttt tttaactggt gggatcgcat
    19261 tattaagcgg actgtctttt cagctgcctt tgttttttat ccctagcttg ctatttggtt
    19321 ctttattcgg ctgctactta ttgtcactac gttatttcgc tagagattat cggttgctgc
    19381 tgctgaactg gaccaatatt aaccaattat ttaacagagg ttcaggaagt ttgggtttag
    19441 tcgcaactat gatgggttca ttgatcatca gtattattct tatcgtctta tacggaatgt
    19501 tggctttgtt ttatccattt tggttagtga atattcctgt gatcggttta gttctaatac
    19561 tttccgctct atggatcgtt tattatcaac gaacgttttg gaaaaaattt gaataaagaa
    19621 gaggggccgt gacaaaaatc atgtcgcagc ccctttttta cagatagagg gtgttcaaaa
    19681 gtcagtttct cagtattaag tcaattttat gtaaaaatgt gagaagctat ttttacaaaa
    19741 ctttttttaa tactcataat tacttcttat tgctcctgcg gttacttgtc gcaatgtttg
    19801 tgagatattt tgtcatcgct caaggctgaa cgtctctttc acaacctttt ctttgttaca
    19861 cttcgtttat ttaaaaagcc gtttttttcg tttcatcatg attgcgtcac gctttacttt
    19921 gaaattacgg atgttatggt taaagacttc tgataataca agacctaaag cgattgctcc
    19981 agcaatcata attgcttgga ccgcataatg agccgcttct cgataggatt cagtaacgag
    20041 atttcgaacc gcttgataag ctaaaccacc agggactaga ggaaccatcc cgggaatatt
    20101 gaaaatcgtt acgggcattt tcaaacgttt agagaaaaga tcgctgatga atgctactcc
    20161 taaagaacca agcaatgaac caaaagcagc agatccgcct atttgaacac agatccagta
    20221 gatcatccag ccaaaagcac cagaaagacc acaacaaatc aatgaacgcc taggtacatt
    20281 tgtaataacg gcaaatgccg cagtcgctaa aaaactaaaa gaaaactgaa taaatatcgt
    20341 ccccatactt gtgttccttt cctaataaaa aagctggaaa ataaaagcaa tcgcaaaacc
    20401 gatcatcgta gcagtcatca tcgcttctgt tccgcgagaa actcctgaga cataatgtcc
    20461 agctagcaaa tcacggatag cgttcgtaat ttgaactcca ggaactaaag gcatcacaca
    20521 tccgataatg attagatcct gattgcttcc taagccaaaa cgtacgctgt agatagcggc
    20581 tatgccaatc aataaagaag ataaaaattc cgataaaaat ttgattctaa aaaatttcaa
    20641 actatataga taagctgcgt atcccaatcc tccaatgact aaagtcacag gtaaatcggt
    20701 catctttccg ccaaataaca ccataattgt tccactgatc actgccgcgc taacgatttg
    20761 caaccagaca ggaaagaaag aacggtctcg gtcgattgct tgcagttcgc agtaaagttc
    20821 ttctaatgtt agctctgaag caacatattt acgtgaaaga tcatttactt ttactacttt
    20881 ttctaaatta atcgaacggt tggtaatttg ttccattcgg atggtcgagg tacgatcaag
    20941 accaataaag agaccggttt gagtgacata gctgaccaaa cggaaatttc cagaagctaa
    21001 agcaattcga ctcatcgtat cttctacccg atacatctcc gcattgcttt ccatcatgat
    21061 tttcccagcg agtaaacaag tgtccagcaa cagatctaat tcttttcccg tcatagcatc
    21121 ttctccaata attgattgat tagttttatt ttactacaaa acataccaga agcgtaaaga
    21181 ataaaaaaac ttgaggaagg aataaaagag gcgtatactg tttttgtatt gcgggtgtag
    21241 tttagtggta aaatcccagc ttcccaagct gatgtcgcgg gttcgattcc cgtcacccgc
    21301 ttaaaatagg ttgtgaaaaa gctgctctgc aacaagtaat aagaacagcc aagcaaaaac
    21361 aacttcttat gtttttaact tggatggact tattaccgca gatgcaagct tttgcgcacc
    21421 gtttaccaag cggttgtgaa atcagtgtat tgtcttgagt atgaagaaaa tttttgaata
    21481 ataataaata ccttacagat agagtggtga ctgattagag acgaggaatc cgtctctttt
    21541 ttgtattttt aagccatctt aaaacaggtt ttattttttg ctgtttctat ttctttctag
    21601 tatttattta taggggaaca tatgttaaga tttagggagc tgttagtaaa ggagtaaaga
    21661 aatgagcaaa aaaacaacca aggcgatcgt ggggatcgtc gctgctgtga ttgcaggggc
    21721 attcggtgtt tccaccacac aaaaagattc agatatcttt cagcagatta caactatttt
    21781 tgattccaca ccacaagctt ctcaagtagc ggatgtgcca gaatatgatg gaaaacatca
    21841 agaaatagaa atcaatggga atcagccgga atttacaaaa gaggatctta gcctttccaa
    21901 aggaacatgg gaaagctatt caaatattga taaattgaac agagtaggac aagccaatgc
    21961 tatgcttgga aaagatttgt tccctactga aaagagagag cctttgtata ttgacccgac
    22021 aggttggaaa caaaagaaat taagtgacgg gcaatggtta tataatcgca gccatttaat
    22081 tggtttccag ctgactggtc aaaataacaa cattaaaaac ttgatgacag ggacacgttc
    22141 gttgaatgct ccatacatgc ttgctcacga aaatgatatt gcggcatata tcaaagaaac
    22201 aaatcatcat gtacgctacc gtgtcactcc tcattttgag gggaatgaac tggtagccag
    22261 gggagtgcaa ttggaagcag aaagcattga agacaaaaaa attgaattca atgtctttat
    22321 ctacaatgtt caagatggct acacgatcaa ttatgagaca ggacaagcaa caaaaacaaa
    22381 aaattagtag cagctattcg aattttttat gaatattatg aaaaaattgt gtaaaaacat
    22441 acattcaaag aaatatagcg tatactttat gaaaagagta cttttgataa agctacagaa
    22501 aaaaggaaaa aaacatacaa atagtgtttc ttaatttatt cggaagtttg gaatatttgt
    22561 ttgtagtttt ccctttcttc gagtaaaata aggttgtaga aggtaagctg gaattacctt
    22621 gcgggaaaga ggtgtagaat catgggtttt acagatgaaa ccgtacgttt tggttttgac
    22681 gatagtcgca aaaaagaagt gagcgaaaca ttagcgattg tttatcgggc tttggaagaa
    22741 aaaggctaca atccaatcaa tcagattgtc ggctacttgc tttctggaga cccgtcctac
    22801 attcctcgtt atcgtgatgc tcgaaatttg attcgccgtc acgaacgcga tgagatcatg
    22861 gaagtaattg tcaaggacta cctctcaaat catggagtga atttatgaga gtaatgggct
    22921 tggatgtcgg ttctaagaca gtaggtgttg ctatcagtga cccctttggc tggacagctc
    22981 aaggagtaga gatcattcgg atcaatgaag cagaagaaga gtttggcttt gatcggttga
    23041 aggaattagt agcacaatat gaagtagacc ggtttgtagt tggattacct aaaaatatga
    23101 acaattccat cggaccaagg gcagaagctt ccatgtcata cggagaaaag atcaaagaac
    23161 tattctcctt gccagttgat tatcaagatg aacgattaac cactgttcag gcagaacgta
    23221 tgctagtaga gcaggcaaat acttcaagag ccaaacgaaa aaaagtcatc gataaattag
    23281 cagctgtgat gatcttacaa aattatttag acggctcagg taagcaatta ttttaataat
    23341 aaaagaaaag aggtaatctt atgacagaac ataaccattc ccacgaccat gaaggacacg
    23401 aacacatcac attggtggat gaccaaggaa atgaaacatt atatgaaatc ctactaacag
    23461 ttgacggaca agaagaattt ggacgtaact atgttctttt atatcctgct ggtgtttcag
    23521 aagatgaaga tgtagaatta caagcttatg catacatcga aaacgaagat ggaacagaag
    23581 gcgagttaga acaaatcgaa acagacgcag aatgggacat gattgaagaa gtattcaata
    23641 cattcatggc ggaagaagaa gaatagacta taaagctagg agaaaccttg atatgatagg
    23701 gtttctcctt tttttatttt ttactgtatg ccatattgta tgccatctag tcaatattta
    23761 agtgttgtac taatttttca acagtctttt ctttcgtttc ttttgccaag tgaccgtaac
    23821 aatcgatcgt ctgactaatt tttgtatgcc gtaatctagt ttgaacttct accatagtag
    23881 ctccagcgtc caagagcatt gaagcatgag tgtgtcttgc tacatgcaca ccgagaaatg
    23941 gaaccatagc tttttcacaa atagtaatca atcttgcata aatagcgtgg tgtgtgatta
    24001 gttctttata gataccacaa aaaatcatag tcggatcagt tacaccaagt gtcagcatat
    24061 atttccgctg attaatctgc cattttttta atatagaaat tgttctttca tctagtggaa
    24121 gaaccccagc actacaatca gttttaggat cactgatttt atagccattt ttggattggc
    24181 ttagtgtttt attgatggtg accgtatttt cttgaaaatc aatgtctgac caattcaatg
    24241 caagggcttc actgatccta accccgctat aaaagagaaa gcgtagtaac gttttatcaa
    24301 agtcgtttcg ataagtttca ggtttgctgt ttaggtaatc aaacaaggtg atcagttgtt
    24361 ctttactgta aactttgacc ttttctttat gcttagtggt ggttctttta ggcattaata
    24421 cgttgtctag tggattagtc tcaataatcc caagattgat agcgtagcta aaaatacgct
    24481 tagaagttga atgaagtacc ttgtattggt cagtagtttc agcccagcta tttacaattt
    24541 gttgggcttt ttttaccgtg acttcattca cacgaagatt tccatatttt ggaagaatcc
    24601 actttctaaa cttgatttca gttttcatga aggtggcttc tcggacagtg gttttatatg
    24661 tttctagcca caagtcataa acttctttaa acgtttcgtt gttcgctttt ttaataccgt
    24721 ttttatcgta gtccacttgc aagcgtgaca aggcaagctg ggcttctttt tttgttttgt
    24781 aacctcgtct acgaacctta tcaggtttgc cagtcatttt gttcattcca agataggctt
    24841 catagagcca aaatttacct tttttagttt catattgctt gaatgttgcc ataatagttt
    24901 tccctccata gtgtagcaag ccgtgggggc ggttggagga ggcagtttat tattattttg
    24961 aatagtgttt atttagctca gtaacaagag ctgtgaactc ttttagaaga tcattttcat
    25021 aaaattcttg atactctttt ttctcatctg tatttttttc tgaatcatta attaatgagt
    25081 catatcgtaa aagcgtattt agaagagctg cgagataagc aattggggta ctcccgtcag
    25141 gaaattcaat tcgagatcct gttttaagat attttattgc gtttgttaaa taaatcttct
    25201 gtgagacagt caggttatct tcttcgatgt tattgatagc attagaaaga ttagaatcaa
    25261 ttatagagtt atttttttca atagtttttt tcaattgctt ttcaagcaca tttcgcttaa
    25321 tttcgggcat catatgcata tctcttaatg tataagatcc gtttaataga taggtagtgg
    25381 atacatctaa tacctttgaa agttgtttaa gcctggtgtt attgggaaga ttatagccat
    25441 tttcccaatt actaacggca cctttactgg ccggtgggga taatttacct ccaagttgtt
    25501 ccattgagag tcccttttca attctagcca gtctaattct ttgtcctgtc tccactgctt
    25561 cggggtattt ttttctagcc acattttcac caccattcat atattttctc tactatatca
    25621 cgatttaaat atgtgtgaaa cataaaagtt caaaaaagta tgttaaaatt cttgcaaatg
    25681 aaaaggtatg atggtatatt gagtttataa gaggtataga aatgtattca agagcctctt
    25741 gcattttttt aaaatctttg tatagaaatg tatgctaacg aggggggata aatatggaat
    25801 taaaaattga caaactcacg cttgatgatc cagcttttaa aattattgac gatcgagttg
    25861 ttcaacaagt agagcaaact caaggaacgt tactttcatt attagaaaaa aagtaccagt
    25921 ttccaaaata tatgaacaag gcacaagctg ctaagtatat gaacgtttca tacaatacta
    25981 tgatgcagaa atatgttcct aacggtttaa agttgattat agttgatgga gtcgtaagaa
    26041 tttcacaaga tgaatgtgat cgctttatgg aagaatacca aaaataaacg caagccgtgg
    26101 gggcaaaggg attaatagga ggaaataata atggaatcga cacaaaataa agcaatcgaa
    26161 aaagtattat caacaattct tacagaagac acagcggcag aattagcaaa cttggaaggc
    26221 aaagcgctag aagaaacgtt tgaatgtttg tacgaacaaa tggactatca aaaattgcta
    26281 ccacaagaac caacggtaag cggagtattg cgtggcttga acgatttagt acaagcagag
    26341 tttaaagaac gactttctat tgaagagtat caagaaattt tgtatcagca agttgagttg
    26401 ttagcgagct tgctagggct tgaactggag gacgttgaat gaaaactgaa atttggaacg
    26461 gacacattat aagatttgtc gatattaatg atgaatggtg ggcagtggcg aaagatgttg
    26521 cggaagcgtt aggtcttaaa caggttacta gagccattca ctctttgcct aaagatgggg
    26581 ttactactag taaggtcatc gattcattgg gcagaacaca agatgtaaac atcatcaacg
    26641 aaaaaaaata tttaccgcat ggcattcaaa agccgtaaaa aagaagccga ggcatttcaa
    26701 gactggattt tcgacatcat caaagaacta cgccagtcca caggacttga aggctttcaa
    26761 gtattccgca tgctagacaa agaacaccaa aaagaagcaa tgactaaatt aagccacgct
    26821 attactgaac caaaaccagt agactacatt aaagcgaatg tgatagccaa taaagctgta
    26881 tcaactattt atggtcattc caaaatggta aaaaagaaag atatgactcc tgaaatgttg
    26941 gttgatcgtg aacctatttt agatgagaca gtagaattaa tgactgttaa agaaaaatat
    27001 gggttgcagt tttcagtgag tgagaaaatt tataatcgtt ctgctgaatt gcagacaact
    27061 taggagcatt aacatgatcg agaacaaaga aaaagcctta tccataacag tcttggcgga
    27121 caggataaag gcaacttaat tgggataaaa cccgatattc tttgttccga ttataccata
    27181 ttaaaaaatc ggagggaacc attatgaaac gaaacttaat tataggaagc attttaatcg
    27241 gcggaatttt cggcttatta ccattaattg caaaaatagc tatctttgca ggattagcgt
    27301 tatatctctt cactgcttat gacgagtacg actatcaatt aagaactgga gggcataagt
    27361 agatgtacaa aacaaaacta ttaaaccaac tcgatagcct tgaattagag gaaattaatc
    27421 aagggatcgc tgaactagaa aataatatcg ggaaaaccta ctttggaaat tcattcaatg
    27481 aaaaactaac agttttgtat gtactcaaaa aacatgcaga acacaaaata atttgtcgag
    27541 aaatcaacga gctaaaaaat cagattttaa ccgcatggtt aaacattact gatatgcaag
    27601 aagcaagggt gaaaacattt aatacatggg taaagtatca gaatcaatta aaaggtgctg
    27661 aatttgttcg ggatgggttg aaatacgaac ttgagcagtt gaagctcatg gaggtgtctg
    27721 aatgatttat accagtattt tgaaaaagga ggagaggttt attggcaaat aattatcaag
    27781 cagcaacgga attacaaaag tcgggatggt caatttatcc cttatctcct ggaactaaaa
    27841 ttccaataaa aggctctagt ggttggaatg atgcaactaa cgattctaat caaattaatg
    27901 aatggtggac ggaagatgca actagaaata ttgggttaat gcttgaagat catcaaatgg
    27961 tggtagttga cctagatcaa cattctgaaa cacgaaacgg cgttgagaat tataaaaaac
    28021 ttgcttcaat gtatgatcca tttccttcta cttatacgga aatcacacca ggtaaaggaa
    28081 tccatttctt ctttaaaatt cctgatggtg taattatcca acaagaaaca ggtgcatttg
    28141 ctgatatctt cgggcgtgat gataaaggta aaagtttaag cgggattgat attatcacta
    28201 aaggagttcc aatagcgcca actatgatta tttcaagaag tgttgggaga gcatatgaac
    28261 ctattgaaaa cgtatctcta aataatattg caccagctcc cgattggcta gtgaacctac
    28321 tgctaaaaaa accgcaagaa ataaagactc aataccgacc cgtagcaaaa acagggacag
    28381 caaaaaaatt agatatgatt gttcaaggcg ctggcgaagg tagtagaaac gatcatttaa
    28441 ctaagttatg cggttggtta ctatggcacg gtgtagataa tcaaacttta gcagaattga
    28501 tttatacagc aaatgcgtac aatgaaccac cgctaaaaga taaagaagtg aatcaaatta
    28561 ttcgatcgat gattaaaaaa gatttgaggg ggaaagctca tggctcataa gcataaatta
    28621 gatagtattt tagactttcc cgaagccagc gagcgtgaag acaatatcat tgaattgaaa
    28681 acctggatgt cacgattaag atgcaataaa gacgatcaga ttaaaagtaa cagtgttgtg
    28741 aatgcagaat tgattttaac caatgatagc aatttagctg gaacaattgc ttataacgaa
    28801 tttagcggat acattcacct attaaaagat tctccttgga tcaatcgtac cgctggagaa
    28861 tgggaagaca gttttgagga tgccttaacg gcatatattg aagaaaacta caatgtagtt
    28921 tttgatgaca acaaaataca caaagcagta gtaaatgtgg ctagaaagaa tgttttcaac
    28981 ccagtaaaag aacgtatcga aaaagttaaa tgggatcaac agcctagact ggaaacactg
    29041 tttattgacc ttttaggggt agacgacaac ctttacacac gagaagtaac aaaacgttgg
    29101 atagtcggat cagtcgcacg tatctataaa ccaggaataa agtttgagat cgttccagtt
    29161 ttggatggac cgcaagggat tggtaagagt accgttccag cattacttta cacggatgat
    29221 tttttcaccg attcattaga ttcgctaggt gaaaagaaag atgattatat gcagcttcaa
    29281 ggcaatgtaa ttattgaact tggagaatta tcttcaatga acaaaactaa aattgaacag
    29341 ataaaaaact tcatcagtgc caagatcgat aaaattagac ctccttatgg tcggaatgtc
    29401 atagcttggg caaggcaatg tgtatttatt ggaacgtcga atgatgggca gtatttgaaa
    29461 gatgatacag gaaaccgccg tttttatcct ttgccttgta agaataagcc aaagatgaat
    29521 ccgtttaaaa caaaggatga atattttctt caagtgttag ctgaagcaaa ggtgcttttt
    29581 gataaaggac aacgaatcta ttttagtccc gatgaagata aagaagtctt agaaattgca
    29641 aaagactatc aagaggatgc caagatggag aatccaatta aagaagcaat cactaaatat
    29701 ttggaaatgg aaatcccgta taaatgggaa gaagctccag cgtgggcgag acgttcttat
    29761 tatcagaact atcctgacag taaatgtaat gagaaaaatt tacagcattt ctataatgga
    29821 gctactatgc aacaagagtt ttatttagtt gatagcgtac tcaccgctga tattttggaa
    29881 gctgtttttg ataaacaagc aaaggattta ttgaatggtc gatcagatgc agaaactaaa
    29941 aagattgcct tgattattag cagtattcct ggttgggaac ggaagcaact gccaaaaaga
    30001 aataaacgac gaggatttta caataagtca aatgcctatg aaaataaaaa tcgagctgga
    30061 aagtacaaaa agtaacgggt agtaacgggt cagtaacacc tagcccgtta ctagtttacc
    30121 cttactgccc caagggatac agcaatttgg taacggggta acaccttatt tccttaaaac
    30181 ttttgaaata gttagctaat atgctgggga ataatgttac tggattttac ccgttacctg
    30241 ttaccaaaac gctagaaccc ttgtccccgt tgggattgag tggtaacggg tacctgttac
    30301 gtaggtgtta ccacgtgtta cgaatcaagg aggaaagtaa atgctcaata ttatttcaac
    30361 aaacaaagca ccgaactttc aatatacaga tgaaatggat cggtttctaa tgaacacgct
    30421 ggcatttagt gtgggattgg taacagaaga ttattccaca tttgatccag aagtattaaa
    30481 gataatggaa gaagaacctg attggctgca ggagtcagta gcgtggtgtc aatcgctagt
    30541 agtgggatca ttggtagata gtggcaacta tgatgataca ggtgagctaa tggatgaatt
    30601 taattgtctg cttaacctat atgatcgagc gagacaacga gagcttacat cgaatgagga
    30661 taatttgttt ttaaacatcc atgataagtt cttggctttg ctactaacag atgatgaatt
    30721 gataactaat ttattggagg tagaataatt atgcatatgg aaagtattag atacttcgat
    30781 ggtgagaaac acattgagta ttcaaacact caagaagatg tagactttat tagttttgag
    30841 gaagataaaa tcactaaagt taattttaaa gacggaacgt gcttgaagat agtttcgcct
    30901 tatattgaat acaaatcaaa atgggaacaa gatgattctg acgaacgaga tcgctttggt
    30961 tggtaatcta tggcagttaa aaaacaatgt aatcatgctg gatgtaaaac attgattgat
    31021 tataggcaga agtattgtgg gaagcataaa gcaaagcaaa cggcaattaa gcgtgaagaa
    31081 agaaagcaaa gcgaaggtaa atattttcaa ttctatcaaa gtagaacatg gcgaaaggct
    31141 tcatacttgt atcggttaaa tcatccagta tgtgaggatt gcctagaaga aggcttgata
    31201 aggaaagctg atgttgtaga tcacaaaatt gaactaaaag atgattggaa tagacggtta
    31261 gatgaaacta attttcgttc tttatgccat gcacaccata attccaagac agccaatgaa
    31321 agacaaagac gagaaaagag taccctttga gttaaggggg catagtgtga aacttactga
    31381 caatcgatgc ctactcattt tggtacaaat aaccgttgtg aaaaggcata agggtaatta
    31441 caaataacga ttacacttgt aatcacaagt gaaaaagtat ataatgaaag taggagattt
    31501 attgaaaaat acaattgtta ttactgataa aacagggaaa cagcgcacca ttgagttacc
    31561 tcattttggt caagtgacca ttcaaatgca gaacggaaaa atcatttata tagataaatt
    31621 agataaagaa aaattctgat cgaaaaacga aggaatgtga gcttaattgc ttacgttcct
    31681 ttttcttttg tctgaaagga ggacaacatg ggacacccta aaatattaca cgatacaaaa
    31741 ggcaatatat caagtgaaga aaaagcaatc cgtgtggacg ctagagaaga attgttcaag
    31801 cagcaaccat taataaatat cacgccccct gactggatgg cagcgagcgc tagaagtgaa
    31861 tggaatcgta tcgtaccaac attaaaaaaa gattatccat tgagtgaagc agattatggt
    31921 tcattggtag cctattgttt agcctttgct cgaatgaaaa cagccgaagc cgagataaga
    31981 aaatcaggta cgtttatcac atgcgaaaac ggagtaaaga aagccaatcc agcggttcgg
    32041 gttcaatcac aagctatgag tgatttgaaa aaacaagcca cctcactagg tatgacctta
    32101 gaatcacgat caaaactagc tttgaacaag gttaaaaatg atgaacccga agacccgttc
    32161 aaagagttga tgaattcatg aatgattaca ttgagaaagt cctatcaggt aagctgattg
    32221 cacccaagaa aattatccaa gcgtgtgagc gtcatataag cgatttggag cgttctaagt
    32281 cagatagctt cccttatgtg ttcgatgaag aacaagccac caaagcgatt aaattcattg
    32341 agttgctacc atctacggac ggtaaagcaa tcaagatgtt aggatttcaa aaattcattc
    32401 taggcagtct ttatggctgg cgtactaaag aagggaatta caggcgattc aatcgagcgt
    32461 ttaccagtat gagccgtaag aatgggaaaa cgtatatcgc aagtggcatg gctgccaatg
    32521 cgttgattat ggaacaagaa ccagcagaag caaggcaagt attgtttgta agtaacgctt
    32581 tgaaacaagc taaattgggc tatgatatgc tgtctaattc acttagaaac gtggtcaagt
    32641 ctagtaagtt tttaagacca caactgaaaa ttatgaactc taagattcag cacttgccct
    32701 ctaattcgtt cgctatggca ctggctagtg aaaccagcac gctagatggg tttgcaccaa
    32761 caaccgcaat tcttgacgag tggcacgaag caaaaactcg taaaacgtac aacgtcatta
    32821 agtcaggaat gacccaacaa aagaacggct tattgtgtgt tattagtacc gctgggcttg
    32881 atttaaacgt tcctatgtac gaggaatact tattgttaga tcgtgtgcta aaaggcgaag
    32941 aacaagccga caggtacttt atagcgatat gggaattgga cgatcccgaa gaaattcacg
    33001 atcaagagaa atggatcaaa gccaatccga tttttgaaag tgaagaaatc aaaaaagtaa
    33061 tgattccaac cattcaagat gatgtgaacc ttgctttgaa acaaaataac cttaattctg
    33121 tattggtgaa aaacttcaat ttatggagac aagcgagtga ggacagctat atgattgctg
    33181 aagactggca ggcaaccgaa gtagaaccac aagatattac aggcaagccc gtttatatcg
    33241 gagtggattt atctaaaaca gatgatttaa ctagcgtttc atggatcgta ccgctagata
    33301 acggcgaact ttattgtgat tcacatagct ttgtagccac caaatatggg cttcaagaca
    33361 aagaaaagcg cgacggtttg ccttatcgag aactagaaaa agctggtgag tgttccatta
    33421 ctcaattaga aagtggaatc gtggactatg accaagtatt tcagtttatt caagatttga
    33481 ttcaagaaaa tgatttggaa tgtatgggga tttgttatga cccgtataac gctaattcgc
    33541 ttatcagtaa agctgaaaaa gccaactacc caatgttaga agtaagacaa ggaacgatta
    33601 ctctaaacgt tccgacccga acttttagag aacaagttta tgaaggcaac gttattcaca
    33661 ataaaaatac gattctcacc catgcagtga acaacgctat tttaaaaacg gataacaacg
    33721 gcattcagat taataaatca aagaacagta acaaaattga tccaatagct gcattaatca
    33781 atgcctatgt gtttgcaatg gattacttca ccacaacgga aggagcgaaa gcagacaatg
    33841 aattttatac aagtgaagaa ttttctttct aattacattc ataccgttct tttacttctt
    33901 ggattggtgt gtgtgttggt tgctatcacc ttactaacaa atgtctacta tggcttgtta
    33961 gcgctgggca tagtgcttat tggggtagcg gtcatgctaa atacagaaca gaagggaggt
    34021 taaaagatgg cattttttaa agcgagacaa aatacaacgg gagatccttt cctacaacat
    34081 gtggtatcaa tccaatcgga tgattacacc accagcttta caagcgttcg tgcattaaga
    34141 aatagtgatg tgtttgcagc cgttcgaatt attgctagtg atattgcttc aagtccgatt
    34201 caattggtca aaaacaatat gccacaagct gataatgaac tggtgaagtt actaaacgag
    34261 aaacctaatt cagaaatgga tggttggcat ttaaagtttg ctttggcggt caatatgttg
    34321 ctaaacggta atagctttgc agagattaaa cgcaacggtg aaaaggtaga agaaattcac
    34381 ttattaccta actcaagtgt aacggttact caattagata atggtacgtt gtcttatcag
    34441 attggtgata aaaaaagacg tgtgaagtct agcgatattt tgcacttcaa atatttcacc
    34501 caagatggtt taacaggatt accaccactt tatgcgttac gtgatgaact gaaaattcaa
    34561 caagctggca accgcacatt gcacaatttc tttactcgtg gtgtcagtgg gtcaggtatt
    34621 ttgaaggttc ataagtcaga tttagacgga tcagcaaaga acgcaattcg tgaaaagttt
    34681 gaagaagcca acggttcaag tagtggagat aatgctctta gaacgatcat tcttgatgaa
    34741 acaatggact acaaaacatt agaaataaac accgatgttt tgaagcttat caattccaat
    34801 gattggaaca caaaacaaat tgccaaggcg tttggtgtac cgattgagcg tttaggcgtt
    34861 gaaaatgaac attctagcac cgttcaaagt aatctccagt atatccaaag tacgctgatt
    34921 cattacttta atgtgtttgt gagtgaatta gatacgaaac ttgaaaccaa tatccgcttt
    34981 aattccgatc agttactaga gactgatcct gaaacaaaag taaaaaatgt attggatcag
    35041 gtcaaagggt cacttctcac gattaatgag gggcggtcga aaatggggct accccccatg
    35101 gatggtggcg atcgtttact agcaagtttg aactttacgt atttagatac gttggagaaa
    35161 tatcaattaa aagaacagga aggagttaca ccagttgaat aatgaagaag aaaaggaaaa
    35221 acggctgaca gaagaagctg agctaacagc cgattctccc aaagtagaga aagaaaatga
    35281 aaaacaacca acagacggca aaactatttc aggctatgcg ttgaaattcg ggcaaccgtc
    35341 aaaagattta ggcggctttg tagaagtgat tacacccgaa gcattaaaag aggtggattt
    35401 atcaaatgtg ttcttattat atggacacga ttattcaaag ccactagcta gtgtcaaagc
    35461 aggtacgttg aaattgaatg tcgatgatac aggattacat tttgaagcag agctaaccga
    35521 cacgacctat tcaaatgatg tgtatgagaa tatctcaaaa ggggttgttg attctatgtc
    35581 ctttgggttc gtgcttggat tagattcatt cgacaaaaaa gaagatggca caattgaacg
    35641 atcaatagat aaaatcaaag cacttaatga aattagtgtc gtgaccgttc ccgcttatga
    35701 ttcatcaaat gtccaagtaa ataagcgttc ttacgaatcg tttatgagta acaaccaagc
    35761 aaaacaaaca aacaatagct tagaatccac ttctaaagca caaaaggaga gtaataacat
    35821 ggaaaaaact ttaatcgata acgaaaaaac agaattgcgt gggtatgaag aatatatccg
    35881 ttcacaaggc gaagtgcgtg atggagtcac tactgtaaat gcagcggcag ttgttcccga
    35941 agaagtaatc ggtgaagtct ttgatttgaa acgttcaaat tataacttag ctcaatatgc
    36001 aacagtaaaa acagtatcaa atggacaagg taaatatcca gtagcaacta accaacaagc
    36061 agtgttagca acaaaagctg aacttgctga aattggtgat attgacgctg aaatgtttac
    36121 ttcagttgat tataaagtag aaactcgtgc tggtaagatt gccttatcaa atgaggttgt
    36181 ggaagattca gcagtgaata ttgtacaaga ggtcaaagat cagttagcga aattggtaga
    36241 aaacactgac aataagcata tcatggattt attaaaaaca ttcactaaga aaacggctgc
    36301 tacgttggac gatttgaaac aactatacaa tgtggcatta gacccagcat taaataaaat
    36361 ggtaattcta aaccaaagcg gatattacca cctagataca ttgaaagatt cagatggacg
    36421 ttacatttta caacccgatg tgacagcacc tagtggtaaa tcattattcg gtatgccagt
    36481 agtattgatt gcagatacat tgtttgccaa tcctaaagct ggaacattcc ctatgattat
    36541 gggggatatt gcacaatcta tctttgttgc tcgtagaaat caagtaacga ctcaatggga
    36601 aaaattcgat tactactcac aaggacttgc agtgatcgtt cgcaacgatt ataagaaaat
    36661 tgatcaaaat gcttcagtgt atattgagtt tacgccagtt gtaacaccaa aagtatagaa
    36721 aaatattggg cggcggttta tcccactgcc tttttttcat taggagagat aaaatggtaa
    36781 ttttggatag cataaaaaaa agtatgcgga tcgatcacac tattgatgat gactttattc
    36841 aacaattgat tgatacagca ggcgaatata taaaaagtgc tattgatagt agcgcaacgg
    36901 ataaagatat ggataattat cagcaatttg atttggcggt gtcattactt actcaacatt
    36961 ggtatttgaa ccgtcaagaa gccagcagcg aacggatacc agtaacggta caagcattag
    37021 tacaacaaat gagaggtgct tattatgcca atcattaaga atgtaaatga attgactgag
    37081 agagtcaagt ttaaaaagac aaaacgggta aaagatgaag atgggcagat ggtggatagt
    37141 gaagaaactg tatttgaatg ttgggctaat gtgcgttcac aaatgctaaa agacgtgctt
    37201 gctagtgtag gtaccattct tgaaggaacg ttgacgttta ttattcgata cgatcaagat
    37261 tatgaactaa caaacgatat gaaagtagct tggaaaaaca agagttataa aattatttca
    37321 atcaatgaag gaacggcgtt taaagattat acaacgatca tagctaaaca gatttcttag
    37381 aatgattaca cttgtaaacg ttgtgggtat ttagtataat taaggtagta aatgaagggt
    37441 tagctgcctg aattttgcaa gacttagcta gtcgaaatct attgttggac tgaaaattgt
    37501 aggtgtggtt gcaaacatgc tggactaaga aagatgatag atgatgaacg ttcaatttta
    37561 atacaggcat gctaggactg attggatggt tttggcaagc tgattgtgta gaaatcaaag
    37621 aacacgtctg ttgtggacgt gttttttttg ttataattca aaagaggtga tttaatggac
    37681 aaagcagagt ttacaaattt ttattggaag tattatctac atttagaaaa tgagtttatt
    37741 aataccacag attttgtgat gctcgatgag aataatttca aaacattctc tattgaatat
    37801 caaaaactac tactagcgat tggttcggag tgtgagatta ttttcaaaga actttgcgga
    37861 tttgatagca gtagcaataa aagcataact gattttaaag ctgttattaa atctagcgaa
    37921 ttaatgtgtt tggataatgg tgttagggtt atgaattctt taacattgac ttcactaaaa
    37981 ccttttggag atgggtggcc tgaagataca ccagattggt ggaaaatata taacagagta
    38041 aaacatggtc gttcttcaaa ttataaggaa gcaaatttgg aaaatgtgtt gtatgcttta
    38101 gcaagtctat atttattaga ggaatatttg cataagaaag taatactaga aggtgaagta
    38161 gactttataa gtccagagtc aaatctattt acgttatctt gggaaagaaa acattcgtcg
    38221 atgcaaaaag tgacgtttga aaaagtggat tcaaattatg tagctccact cattgatttt
    38281 aaagaacaag aagaaattta aaaaccccca cggcttattg aagtttttgt atgccatatt
    38341 gtatgccatt tggtttaaat cgcttgaaaa atagtgatag ataactctaa ttcatttagt
    38401 cataaacgct gttttgataa cctttatagt tctttgatat gggtttctca ttcatggcgg
    38461 aagaagaaga atagaacaaa agaatgaatt aataagatat aaaaaaaggg attgtgacaa
    38521 acgtcttgtc acaatccctt tttccaaata aacgatgttc aatcagctgc ccctcggcat
    38581 tcgctcgaaa aacggcgaaa tatgaagaac tattttgcca tttgtttctc taataatcag
    38641 gtcaaaacgt tgacttcaca gtctcttcct taaacggtgt tctcctgcca actcctcggt
    38701 cacaagtcac aatattcaac aaacatgaga agctgttttt tgaaattgtt ccttgtgact
    38761 cggagctgaa cggcaggctc acgacctctt tcttaaacgg tgttcaaaag cttgcatctg
    38821 cggtaataag ttcaaccaaa cgaaaattat gaggagctat ttttgtttgg ctgttcttat
    38881 tacttgatgc aggacagctt tttcacaacc tcttttcgtt tagcactaaa tttccttaaa
    38941 aagtagaaaa gctttcgaag cctcgtttgt ttcttttact gtataataaa agaaaagagt
    39001 aacaatcgct aaaagatata aacaagagaa aggaaataca agatgattgg tctgatttat
    39061 tttgtggtga tcgttctggc gaacacagtt ggcgctattt ccgggatggg tggaggcgtg
    39121 ttgatcaaac cgattcttga tcttatcggc gcccattctg ttgcagggat ttctttttac
    39181 tcaacagttg cggtattcac gatgtccatt gtttcgactg tgcgacaagt atcaagcggg
    39241 aaatcactga attggcagat tgtcggttgg gtatctagtg gagctgttgt tggaggagta
    39301 gtcggcaaca ttgtattcga agttttttta cagttatttg aaaacgagaa gcatgtgcag
    39361 ctgatccaaa tttttttgac ggtattgacc ttggtttttg cgttcttcta caccaagcat
    39421 catcagccaa aatttcattt gacgtcatgg acttggtacc ttatatgtgg gggggttctg
    39481 ggctttttag ctagtttttt aggtattggt ggtggaccag tcaatgtttc tttgttgatg
    39541 ctgatgtttg cattgccaat aaaagaagca acactatatt ctctatctac gattttcttt
    39601 tctcaactgg caaaattggt aacgattgct ttaacaagta gctttatgcg ctttgattta
    39661 tctatgttgt tttatgttat ccccgctgct gttattggag gtttgtgggg agcaagattt
    39721 agcagggttt tgtctccaaa aaaagtgacc tttatttttc aagtaattgt cctcgtggtt
    39781 cttttgatca atctttacaa tgctttcgtc atcttacaaa cattaggaga atctagacta
    39841 acgcaagtca aaatcactga aacatattgt tctaaatgaa aaaaggagta tactgtgata
    39901 aaaaaattca tttaaaaaaa taaaaattgg ctggtgattt ggatgttgcg ggaaatgcgt
    39961 ttaggagatg cagaagcact acgagaaatc aatgcggaac aattaggata tgatgtacca
    40021 ttaaccgtta cggtgagaca aatgaagaaa ctgttgaagg agccagaaaa aaattttttc
    40081 ttggtctatg aagatcctac ttctaaaaaa gcagctggct atgtacaagc ggagtcctat
    40141 gaatcgattt tcagcgatcc gatgttcaat attatggcac ttgctgtgtc taaacatgct
    40201 gaaaaaagag gaatcggaaa ggccttgatg actggcgtag aagaagcgaa aagacgagga
    40261 atatcagcaa taagacttaa ttctgcagaa tacagagtag aagcacaccg attttatgaa
    40321 cgcatcggtt atcattccga taaaatgcaa aaaagatttt tgaaaatcct gtaaattttt
    40381 atcaaaaaat acctggtcat caagccgata cttgatgatc aggtattttt tttgactaag
    40441 aaagtaattg catcaagaaa cctgagatga tcaaaacgat ggcaccacca agccgattgc
    40501 ccatttgagc aaaagcaata agttccatcc gattagctgc agataagaca gctacatttc
    40561 ctgtaccacc catcgagttg ttgcataagc cagcagtaat agttgattcg attggatata
    40621 aaccaaacaa acgaccgaca aatcctgaaa ctactgaaat cgcgataaca cttgtcaaac
    40681 ataagacaac aaattgccat gtcaaagcag aagccaatac atttagattc aataacgcaa
    40741 taccgatacc ggcaagaaca gctgctgtca agtttttgac aatcaagttg ttgaacatga
    40801 tagctgcatc ttcgtaatat tctggtaata ggtttgcgat tttacagata acaacgataa
    40861 tgatcatgaa tgcgtaagca tggactttag gaacaaaata gttacagatt tgtcctaaga
    40921 tgaagaaagt caatgatacc atcaaaccga cacctaattg tggaatggaa ggttttacat
    40981 ttctaggttt tccatcgcca aggtcgcctt tttccattaa tttaccatta ccgttgaatt
    41041 ttggcattga ttgtcccgca cgagcaacta acgctgcacc aatgatagct aggacatttg
    41101 tcattgctgt cgcaggaatc aggcgggaaa tgatagaaga agcgtccgta cctaaaacat
    41161 ttgcataaat cgttgataaa ggtacagctc cggctccaac accaccagcc attgcgggca
    41221 atgagatata taaaacggaa tttgcaaaac catttccgat aagcatacct acgagtccta
    41281 cggcaaaaaa ggagacgacc attgaaataa gtgcaacggg aataaaacga acagaagctt
    41341 tcatcaatag attacggttc ataccaagga tgctaccagt aatcaaggca gcaatataaa
    41401 agtctaaaaa gcccatattg ctgacaaagt tttctgtgga ttttactaca tactcaggta
    41461 agatcccaaa tgtagcaatc gcggcagagg caaagatcgc aaaaacggag ccaccaccta
    41521 aataactttt gacgataggg atttttgttc cgataaaatg aaataaatta ccgaaaatga
    41581 ccaaaacagc gattgggcca atcatattcg tcggtaattt tcctaaagcc attgcaatga
    41641 tcaggacaag gaccatgatc aaataaaggg gcaaacttac gccggtaatt tttgttgacc
    41701 aaaacccttc ttttttcttt tcctcaactg cagttgcggt tgttgttttt tgtgccattt
    41761 ttacagatcc tttcttattc tgtatcgtat gaaaaaagcc aaagagaagc ggcaaaagag
    41821 cagtagttgg ctggctgctg ttttgccgca gtcgcttttt tctttttaga cgtcatacaa
    41881 agtttctgta tgttctttta ctataacaat cttaatcttc taaagattta tattcaggaa
    41941 cccatttagc agattcaaca gcttctttga tatccgtgat ttcttcacga ttcaattttt
    42001 gtgcaacaac tgattttgcg acagtttcag caatcgtttg agagaattca gtgatttttg
    42061 ctactggagg caagatagct gctcctggtt ttgttacatc aacgattccg cctaatgctc
    42121 gactagcttg agaaatgatt tcagcattta ctcgtgtagc tgttgaagca atcaaaccaa
    42181 gccctaaacc tgggtacatc aacgcattat ttgcttgacc gatttggtaa gttacaccgt
    42241 tatattccac atcagcagca gggatacctg ttccaactaa agcttttccg tccgtccatt
    42301 caatcaaatc ttttgctttt gcttctgcca attttgttgg attagacaaa ggcatgatga
    42361 ttggacgtgg ggtatgagcc gccatttctt tcacgatttc ttcagtgaat gctccaggtt
    42421 gggtagaagt accgatcatg atcgttggat ggattgcttt tacaactgct tctaagtttg
    42481 tcaattcatc agcattagtg aattctgagc gtttacgagc gaatgggatt tgaccaggag
    42541 tcaatccttt tgtatcttca aacaataaac cctgtttatc tacttgatag aaatgttggc
    42601 gtgcttcttc ttcaggtaca ccttgacgga tcatttcatc taaaaggatg tttgcgatac
    42661 ctacaccagc agtaccagca ccaaatgtca agatcgtttg atctttcaat tgctcacctg
    42721 atatattcaa tccacctaat acaccagcta gtacaacgat tcctgtgcct tggatatcat
    42781 cattgaaagt agtgatttta tcttcgtatt tttctaagat cgttgcagca tttcctcgtc
    42841 caaaatcttc ccaatgtaat aacaattctg ggaataattc agtagctgat tgaacgaatt
    42901 gatcaatgaa ttcgtagtaa gtttcgcctt ctacacgagc atgacgattc cctaagtata
    42961 atggattatt caataattct tcgttattag taccagcgtc gattgataca ggcaagactt
    43021 gtgcaggatt gataccagcc gcagcagtgt agaccatcaa tttaccgata gcgatgtcaa
    43081 caccgttcac accccagtca cccataccta agattccttc tgcatctgtc acaacgatca
    43141 aacgaatgtc gcgtccatct gctgcgtttt tcaaacttgc tttgatattt tctggttcgt
    43201 cgattgaaag aaatgcagca tcttgtggtt tcaagaaaat ttcgttgtat tgttcgattg
    43261 aatccgcaac gacaggatca taaacgacag gcatgaattc aactaaatgt tcgcccatca
    43321 atttataaaa caatgtccgg ttcgtattga atagattcat taagaagata cgtttttcca
    43381 aatcggtctg tttgcttaag tattgaccgt aagcttggac agattgttgt tccaatgttt
    43441 ggacagtact tggaagcata ccagtgattc cgtattttgc ccgttcttct tttgtaaaag
    43501 ctgttccttt atttagaaaa ggattattta ataagtttag ccctgtaagc atgaatgatt
    43561 cctcctaagt tttaatacaa acgaagtata ggagtcagat gaggatatag tcaaaaagag
    43621 caggtataat aaattttatt tatatgagta atagagtcaa aagaaatcaa aatagcggta
    43681 tttgttcttt ttatatcaac aatccttgct tgattactat gcgtatctct tatatgctaa
    43741 agaaagaaaa gtagaagaat aaaggatatg ttagatagat aaatagaatc tctgaagaat
    43801 aaatcacgtg atcgtgacga taaaatacac ttacgtcagg tgataaaaaa tacataagta
    43861 gaaaactaag aggaggaaca gaaaatgaaa gaagttcaat tgatcagaaa aagcgaacta
    43921 tcagaaggtg gttgcaatgc atgcggcgta gtagaagcaa caagctatac attaaagctt
    43981 ggtgccaaca aagcgataat ctctgagttg acagtaggcg gtcttgtgga cagtctggcg
    44041 cttgctgaag gttttattgg agaagatatt tatgagatgt tttcagaaat ccgtcagtta
    44101 aaaaaagggg aaaactgcat agaggttcat catgagagcc cgaatgtgag attcaaacgt
    44161 ggtgataatg agatgatctt caataatcat gtgtccgatc acaccgagct atatggaatc
    44221 gtgaatcaaa tactaactga gctttttgga ttgggacctt atgcgttcaa ggaagaaaac
    44281 ggtaatccaa aattaaacga agaatggcaa gaaacgattg aaatacaaag aaacaacccg
    44341 cacttatttc aataggcatc cacagtattt cgtaaaaggg actttgacac gacttttgtc
    44401 aaagtcccat cgttttttat ctatttgtgt tctaaacatt tctttcagag tagtaacaac
    44461 tggaatgact gaaatgtaaa cagattattt gcctgtatca atacggaaga gtaatataaa
    44521 gtgtgtaaac gctatcatat tattcattca attttttata gagggaaggc attgaaatgg
    44581 aaaaaacgta tcaagcaggt acaaatgaag gaatagtgga ttttatcaat atggaagatt
    44641 tagaaatagc cgcttcacaa gtgattccag ctggtggtta tggttacatc agcagtggtg
    44701 ctggcgactt atttacttat caagaaaatg aacgggcatt caatcatcag ctgattattc
    44761 ctcatgtatt aaaagatgta gaattacccg atacaactac acattttgac aaagaaacgc
    44821 tgactgcacc aatcatcatg gcaccagttg ctgctcatgg cttagctcat gtgaaggcgg
    44881 agaaagcttc tgcaaaaggt gtagcagatt ttgggacgat ctatacagca agttcttacg
    44941 cttcttgtac attagaagag ataagagaag caggcgggga gaaagcaccg caatggtttc
    45001 aattttatat gagcaaagat gatgggatca atttggatat tcttgaagtg gccaagcgta
    45061 atggagcaaa agcaatcgta ttgacagcgg atgcaacagt tggcggaaac cgtgaaacag
    45121 accgcagaaa tgggtttacc ttcccactgc caatgccgat cgttcaggct tatcaatcag
    45181 gagtaggcca aacgatggat gctgtgtaca aatcttccaa acagaagctt agtccaaaag
    45241 atgtagaatt tattgctgct cattctgatt tgccagtcta tgtgaaaggt gtccagtcag
    45301 aagaagatgt ctatcgttca ttagaatctg gtgctggcgg aatctgggta tctaatcatg
    45361 gtggccgtca actagatggt ggtccagcag cgtttgattc gttgcaatat gtggcagaag
    45421 cagtagataa acgtgtacca atcgtgtttg acagcggtgt acgacgcgga cagcatgttt
    45481 tcaaagcaat cgcatcaggt gcagatcttg ttgctattgg cagacccgtt atttatggat
    45541 tgtctttagg aggaagtaca ggagtccatc aagtgtttga tttctttaaa acagaattgg
    45601 aaatggtcat gcaattagct ggaacacaaa cagtagaaga tatcaaaaaa ataaaattgc
    45661 gtgagaatcg ttttatttaa gaaacaaatg acgaaaaaaa taggagtgcg gcagatgatg
    45721 ccgtactcct atttttatag ttcaaggatg tcactaatag aataatttga aacgaaaaaa
    45781 taaaattgaa atattttttc atacttaata aaaaaggaag aatattcatg taaacgattt
    45841 tattgttttt ttctctttta tacaaaaagg gtcataattt caagtaagcg gatgaattca
    45901 tgaggatttt ttgcatactg tctctactat acttaggtca ctaagaaaaa ggagtgattt
    45961 attatgaaaa agaacatgat tgggacatta ttactttcaa cacttcttct aggcggcttg
    46021 gcaacaccag cttttgctga gggtcaagcg acttcaaaag gagatattac ctttactgaa
    46081 ccaacaaata ccgtggagcc tcttaaccct actgatcctt caaaaccagt agagccagca
    46141 gatccggaaa atccagcaac tggccagaca ggtcctttaa cactggatgt agttcccgaa
    46201 ttgccatttg gtacacacga gattgaaagc ggaacaaaaa cgtatcaagt agacgcttca
    46261 aaaaacgata caccttatct ccaagtatca gatcgtcgag gtgtaggtgc agatggccaa
    46321 gcacaagggt ggaacgtgac agtttcggtt tcagattttg taaacggttc acaagttttg
    46381 caaggcgctg aattagattt tggaacatcc actgttaaaa gtacgtcaga taacgaatcc
    46441 actggaccaa ctagtcaaac tgtaactggg ttgagtaaag caagtgcagc gacaccaata
    46501 tttacagcag caaaagatca aggtctggga acatggcttt ctgtttatga tccagcaaat
    46561 atcacactga aagtgccgaa agcagcagct ggaacattca cggctgattt gacttggaac
    46621 ctcgtggctg gcccagtagc atagagtatg aaataaattc aacaaactgc ggtgaaaata
    46681 ctataggtga aggatttttt accgcagttt ttgttgaaag gatgtggtgg acaatgaatc
    46741 aaagtaaacg actgtttgtt tcgttttttt ctattttaag tctatttttc atttttccgt
    46801 caatcagcca agcaaaggat tcagcaggag actttggaat caaaccggta tttccagaga
    46861 accaaataga taaagcaatc ggttattttg atttattagt ggcaccggaa caaaatcaga
    46921 cactagaagt aattatcagc aattcttcag atgaagaacg aacatttgag gtctctgtca
    46981 atccggcggt aacgagtgat ggtggaacga tcgattatag tcaaaaaaac ccaacgttag
    47041 atgagacatt gccgtttgat gtccgtgacg ttttgctcat tgcgaaaaag gaaatcaatg
    47101 tatcagcaca tgcagaaaca actgtaccta tagaagtgaa aatcccagca aaaagcttta
    47161 agggacgtgt attagcgggt attcatgttt cacctaaaga agaagctgaa acagaaaatg
    47221 ctacagaagg agcacaaatc aaaaatcgta ttgcttataa tctcgcagtc gttttacaag
    47281 aaagtcaaga gacgatagaa ccggatctga aacttttatc aggagatttg gacgaggtca
    47341 acgcaaagcc aactgttcag cttcgtttcc aaaatcctca gcctaggatt atcagcaatt
    47401 tgatatttac gagcaagatc ttttatgaaa atcagcttta tattgaaaat acgtctaatg
    47461 catttttagt tgcaccaaat tcgaattttc acttgaatct tgatttagct ggtgataaag
    47521 caaaagccgg cgattaccgc gcagaaatca tagcaaaatc aggcgacagt aatgaatggc
    47581 gatttacaca aaactttacg atcaaaaaag aaaaagcaca aaaagtaaat gaaaattccg
    47641 tttttgcagt acaagaacaa tctttcccat ggctttatgc aggattggtt gctgcagttc
    47701 tagtgttatg gttgattttg ctgtttttgt gggtccacaa gaaaaagaaa aaaggagaaa
    47761 caacatgatg aggtgatttc gttgaaaaca tcgatcttat tgggagggat tattgcaagt
    47821 ttgctgttct tcagctggca agagccagtc caagcagata ccaaaagttc aggtacgatt
    47881 acattttatg atctaagtcc aattccgcat ccaagtggtt cagtcaatgg cgcatcaaaa
    47941 cagcagacac agctaccgat ttataaagaa tctaatcaac atggcgtatt cccggagacg
    48001 aatgaacaaa agatccgttt tctttatttg atcattggta tagaattatg gcttcttgcg
    48061 tggttgttgt tatggagaag aaaggaaaag gaggatgaac atgttgctac aaaataaatt
    48121 ttcatttttt cgctccttct tttttacttc gctttttgtt ttaagtatat ttatcttttc
    48181 accttcggca gaagctgttg gagcaacgcc tcctgccatt tcttatgatc catacacgta
    48241 tgaaaatatc gtacctccaa atggtggacg tagtgcagtc aatggcggga agcttgtcca
    48301 actaactggg gctaaccaat tgcctaaatt gactgctact tatttgaacg atggttcaaa
    48361 tgatccttgg aaaggtggca cggtccgttt tcattgggaa agccatgtga aactaacaaa
    48421 tgtacttgat ttggtgaata tcactgcaca aggatttgca tttgtggcga tcatcaattt
    48481 gccggaacat gtatcaagca gtgatgtatt aaaagcaatt gactggaaca acgcctactt
    48541 gtatattgcc ggtgccccca tcaaggttac tagcggaagt ttattatcag caggaagcca
    48601 taccttgcgt tttgggatgg ggtcgtggga tacaaatact ctattgacag atattattgc
    48661 tattattacg cgaggcgaat tcaacgtgga cgatatcccg cttcaatttg atgttacagc
    48721 taatgtcgcg aagatgacag aaaacggaga taaatatgat acgagtccaa acaaaatttt
    48781 gaccaagggt aaattacaac cttctatgtc taaaaaggcc gttttttctg ttgattttta
    48841 tgattcgaat aatattatcc aagatcgtat gttcaattcg attttagctc cagctgcagg
    48901 tagattttat cctaaattaa ctggcggaaa cttgaatcca ggagcatatg cattttctgc
    48961 aagtgcagag ataaatacgt ggaatagtta catctcacca agtgattcaa caggaaaata
    49021 taatacattt gctgatacca cagaatacgt ggatggaaat aaccagaacc ttccaggctc
    49081 taccaatagt cggacattga cgatggggct gactgaagga cgttttgatc agttgccatt
    49141 tagtcgcttt gatcgcgtag tcaactattt tacaggaaaa aacgttacac aaggagcaat
    49201 actgactcat actccagcaa gcacaagtgg attgaatcag acagccaata tcgtgtacag
    49261 tgggaaagat gcgcaaggcg gattgctttc tcctgttgct ttaaaggtat tggagcagta
    49321 caagctagac ggaacggttt taccaacaaa cttaacagcc gatagtcagt ggggaaagct
    49381 cctttcttct gcatcgtacc aagtcacggc caattggtca gcaactgaac taaaaaaggg
    49441 ttcgatccat tacaggcttt attcgaaagg aacccaaaaa ttagcgacag gatggacaga
    49501 tcaacttttc caaacgatcc aatcaaataa cgggggtgta aatacagcta cttctacact
    49561 acctgcattg gcttcagggc aatattattt tgattatcgc ttggtggacg atgtacttac
    49621 taacatgtat ccgtcttttg cgtacaaatg gcagtctgaa caagctggaa tcaccactct
    49681 tcctcaaata acagtggcta attttccatc tgtgagtgca gatagtcagc taaaaaacct
    49741 tagcagacaa cctgagacag gagatcccct gtctgctcta tcaggagata cgattcaaga
    49801 aaatgtgaca ttcacgttaa caaaatctgg ggatgcgata accgacaaaa aaatcacgat
    49861 ctcacttcct gaaaatacta cttatatcaa tggttcctta aaattaaatg gtacaactat
    49921 cgcagatacc ggcattcaac aaggagtttc tgtcccagca gatctattga gcaaaatagg
    49981 agataccatt catcttactt acaactatca attaaatact gtagatactt ctttacaatc
    50041 cgtcagcata ctgacaaagg cagctgtact ttccagcaat attacattag cagatggtgc
    50101 taagcttcct aacccagttg tccaaacatc tgctaaaaca attcttgtcc ccaaacaaga
    50161 attaacatta gttaatgtac cagatgattt tacatttggt aacgatctcc ccaaaccgtt
    50221 gaagacaagt tattatgaat caaaaggcga tttttctttt gatgtgcggg atactcggct
    50281 tccaagcacg tctccttggc aactgacagg tacattgact tctttattta agaatgatca
    50341 aggtcaagaa ttaagcggaa caaaattata cttcaatcat tctggatcaa agcaattgat
    50401 tcagcaaggt caaaacacgt tgatttatga gagtgatgga acagctaaag gagaggttct
    50461 cgttgacttt cccgataccg atggtttact cttagaggta aatagcagta cgaatgctca
    50521 acctggcgca acgtatcaag gcatggtcac atgggaactg acagctgggc caaccagcta
    50581 agtgattaga atttgtatgg atctgtgata actgtcacag atcctctttt tcaaggatga
    50641 gaaggaaact tctgctgtta aaatcagaaa tgcttgaagg agtggacttg gaacttttca
    50701 gtttgcttga aaaaaatgaa caacggcaaa tcaaactttt ccaactttta ttagaggaaa
    50761 aaggagaggt ttccgttaat aacgtactta cccgcttatc agttgatcgg gctaccttga
    50821 aagaagatct gttttattta caagctaact tggaaaatat caaacaattg gaaattgtta
    50881 tccaagaagg gaatatttta ttaatcagac atggaaatat agcagcagtc gatgtttatt
    50941 atcattgtta cttgcgctat tctacaaaat atcagatact tctttatctg ttggaaaatg
    51001 gctcatttga tcggcagaaa cttgtcatgg aacttaatat cagttcggct actttgtctc
    51061 gcagagtgaa ggaattaaat gattctttaa atgagtttca tctgaaaata aaaagcgggc
    51121 ggctgaccgg tccagaaagt caaattcgtt atttttattt ccaattattt tggtttggac
    51181 gtccctatct tgaaaacaaa aaagaataca gtgatttatc aggaaacact tttttagatc
    51241 tgctgcaaaa agagtttagt tttgctatta cagaagaagg acgattgaaa tttgaaatat
    51301 ggctttccat tatgaagaag cgaatgcgct gtacgaaaaa atccaaacca gattatagca
    51361 atcttcctgt tggattgttt caaacgacac aatttttatt ggacctccat acatttttga
    51421 cccgttactt ctttcaatat gcctttcctt ggaatcaaaa tgaaacgttg gttttttatc
    51481 ttttcatgat cagtaacttt acattagata cacataactc gcatgtccag gagtttttga
    51541 taagcgaaaa atacaaagac gacgtagtag ctcaattgaa tcgaacgttt aaacaatcga
    51601 tagaagaata tttctctttt tatgtttttc cagaagagat ttcgcaaaag attgcgttga
    51661 cagtgatgca aaatcacgct cattttgcct attttagagg atggatgaat atttttggcc
    51721 aagaaggatt aaaacagcgg ttaactgaag tagaagataa acaattcctg gttttatgtg
    51781 aaagattggt cagacaaaca tttgctattg ttgattttac tgaagtagac gaggaaaatg
    51841 cccgctttga attatttgct cgctatgcat ctattttacg gacgctgatc aagcagttag
    51901 aattacgtct gcaaattgct tatgatcttc cttatgaaag gctaatggcg gatatcgtga
    51961 aagaaatcat ccaggaacaa ttgctgaata cgatcaaata tgatttagtc gaatacgaga
    52021 aagaaaagca gtatgatatc attttgacta gccaattgaa agagtacccg tcacagaagt
    52081 ccgctaaagt ttttgtattc actagcaacg aatataatta tgatttcccc tatttgaacc
    52141 aatttttaaa agaatgttat ttagaaaaat tgaatttaag gatgaataaa acatgaaaat
    52201 cgtgatcatt ggtgcttccc atgaaggcat cacagctgcc ctaaatctaa gaaaacttca
    52261 accgaaagca gaagttttac tgatcgatga agatcataaa gatggactag gctatgtttc
    52321 aaatggaatc aacctttatt taaaaggaaa gatacgtagt ttagctgaag ttgcccataa
    52381 catgcggact cttcaagaaa gtggggcaaa actgatcacc gaatggagag taactgaact
    52441 tgatccagac aaacatcagc tcatcctcgc atccaaagaa ggaaaaaacg agaccataac
    52501 ctatgataaa ttaatcgtag cgacaggttc ctctcctgtc accttgtata aacaaattga
    52561 agctgaaaat gtctacacct ataaaaatct agtgcaatct aagcaagtac ttgccgcttt
    52621 aaaagaagca aaagaagtag tgatcttcgg tgctggttac attggattgg aattagcaga
    52681 tgctttgaga aataaaggat acattattca tctagttgat tatatgccga atgttttatc
    52741 aagatatttc gataaggata tgatcaacag ttttcaaaat cagctgcaaa ctaagcaaat
    52801 aaatttttat ccaaatgaat tcttgatcga ttggaaaaaa tcagaagaga aagtagtatc
    52861 cgtacaactt ctgtctcaaa caataaaggc agatatggtc attttttctg cccaaactcg
    52921 tcctaatacg acattgttaa aagaaaaagt ggctttgtat gaggatgaga ctgtgatagt
    52981 caatgaatat ctgcagacaa gtgatccgga catctatgcg attggagata tcgtcccagt
    53041 cagttttgac aaaaataaac ggcatctctt tttaccttta gttactcgag ctgtacatat
    53101 ggcaagagca gtagcgctaa ctttgagtgg tcagccaacc gcttacgacc tcagacaaaa
    53161 gataacagca gcagtcatta tagactactt tttaggaact gtagggctga cagaagatga
    53221 agcgccattc ttggaacaaa ataccggttc gtgttcagga gaatttgatc tatttccaca
    53281 atacgacgaa gaaaacaaaa cagtaaatgc aaagctgatc taccatccgg atacattaga
    53341 aataattggc ggacagctga taagccaaga attcttatta tcagatttga acctgcttgc
    53401 ggatatcgtg aaacacaaaa ctacgattcc acagctagca gttgaggatt ttggatttct
    53461 gtctgaatac acctctcgtt ttcactacct caatgagtta gcttttaaag tactagctaa
    53521 gagagcaaat agcaatagag tagataaaag actgtgagaa atatcacggt cttttattgt
    53581 gtggggatat aataaatatt aatcaatcga gaataaaaaa ttcatataat gaaatagcgg
    53641 aagaattttt tgagcataat attcgccaaa aatttatcaa gtgataaaat tgttgtataa
    53701 aatgattatt tcaggaggag gagttaaatg tttttagcat taaatgaaat tatgcattca
    53761 aagttacgtt atgcgttagt tgcaggggtg atgtttttga tcgcctacct ggtatttttt
    53821 ttgactgggt tagcttatgg cttagctcaa gataatcgaa cagcagtcga caagtgggag
    53881 gcagattcga ttgtgctttc gaaagatgca aattcaaatc tagggatgtc catgattact
    53941 aagaagatag cagaagaagt cgaaggtggg aaagtggcgt atcttgccca aacacctgga
    54001 gtcgtgacta gtaaagacag tacagaagaa ggaaaaatca atgttagctt cttcggtatc
    54061 gataaaaatc aattcatcat gcccaatctt gttgaaggaa aagcttttga taacgatgat
    54121 gaggcagtag gcgatattag tttgaaagaa gaatatggtc tagccatagg agatacagtt
    54181 aagttatctg gtagtgacaa gacgtttaaa ctaactggct ttacggatca tgccaaattc
    54241 aatgtctcac cagttctcta tacaaccatc aatgcttacc aagaaatccg cttcgaaaaa
    54301 gaagatacaa gtgaaaatgc acggattaac gcaatagttg tcagaggtaa aatcagtaat
    54361 ttacctgaag atttagaaca gatcaaaatt tctaaattca tcaatgaatt acctggatat
    54421 aatgcgcaag tattgacttt tggatttatg atcggatttt tgatcgttat tgcggctatt
    54481 gtgatcggga ttttcattta tgtactgacg atgcaaaaga ttaatatctt tggtgtaatg
    54541 aaagcacaag ggatcacagg aggatttatt gcgcgttctg tagtggcaca aacattcatt
    54601 ttatcatttg ttggtattct tcttggtcta ttaggaacag tgggaacatc ccttgttcta
    54661 cctgatgcgg ttccattcca aagcaattgg ctgttttttg gcgtcattag cttgttgatg
    54721 ttagtagtag ctgtgttagg cgcactattt tctgttcgga caatcgtaaa aatcgatccg
    54781 ttgaaggcaa ttggataaga aggaggaaga acatgaaagc aatagaattt aaagaagtag
    54841 aaaaaagttt ccaagatggc gaccaagtag ttaaagcatt gaaagaaaca accatgagta
    54901 tcgataaagg ggaatttgtt gcaatcatcg gtccttctgg ttcaggtaaa agtacttttt
    54961 tgactttggc aggcggattg cagacgccta gcagtggctc tgtatttatc aatgatcaag
    55021 aattcagtaa tcaaaaagaa agcgaacgcg ttaaactacg ttttaaagaa attggcttta
    55081 ttttgcaagc ttccaatctg gttccttttc tgacagtaaa aaagcagtta ttattagtag
    55141 ataagattaa acatgaacat cgtcaaaaag aagcaaacga attatttgag cagcttggcg
    55201 tggataagtt gatcaacaag tatccagaag aactatctgg tggagaacgg caaagggtcg
    55261 caatcgctcg tgcattgtac aatgatccgt ccattattct agcagatgaa ccaactgcta
    55321 gtttggattc agaaaaggca cgagaagtag tcgatatctt ggcaaaagaa tcaaaagaca
    55381 aaaataaagc aacgatcatg gttactcacg atacacggct gattgacaaa tgcgatagaa
    55441 tatttatgat ggaagatggt gtattgaaag aaaaagaata accaaaaagc aggtggaaaa
    55501 atggtccatc gatacaacaa agatacacag caaaaaattt tgaaagaagc gactgacttg
    55561 tttatgtcca agggatatct cggcacttca acaagagaaa tcgcacaaaa agttgggatc
    55621 acccagccca atctctatca ttatttcggt gataaagaaa aactatatac ggcagtttta
    55681 gaaaaccact taaaagatgt tggaaaagct ttgagagaaa tcgttcaaac aagcgaaacg
    55741 ggatttcaag taaccttgac aaaaatggcg cagtttttga ttgagacaca tctggtcgat
    55801 ttgtttctga tgcttcacga tcttgaaagc aatctttcaa aggaaacaag agatcatctt
    55861 ttctttctat ggaagaaaaa ttatcgtgaa ccttttgaag aaatcttttc agcaaaccaa
    55921 tctgtgctaa gaaatgggat ttcaaaagaa atcgcagcaa ggcatttttt cttagtgtta
    55981 gcaccttaca tcactcggtc gaaaagacct gtggaagaac cgctaaatgt ggcgcaattg
    56041 atcgatttat atttgaatgg tgtattagga gaagcgtaaa gctgttatat agggagaaaa
    56101 tagttaggtg aagaagcaag atatgacgaa gatcgttata tcttgcttct ttatataggt
    56161 agtttttcat aaatacaaat ttgttattct ctcatgatta tcagggtata tttttattta
    56221 ttatgtcggt gttccaggaa gtgttttaat ttaataacga agaaattaat tgtttgtaga
    56281 atgaaggaaa ctatttattg ggtagatgaa tcaccacgaa tagtcaaaag gagatttcag
    56341 gatatgccaa aaatgaaaaa aatcatttta gcagtaatgt tattttctac ggttatatct
    56401 tttacagccc cttttttgct tacttaagct gattttaagt tttggccctt agtctggatc
    56461 tacgaggtga gagtcaagaa tgaaaaaaat aattggagct tttgtatttg caatcattat
    56521 cattattatc tgttttcaaa tagatgtttt tggtcaaaaa aagatagttg gacaaacggt
    56581 cagcagtacg aacagtacgg ttacaaagac aaaggaatca tttgatgata cagaacaaga
    56641 gacattaatc aaaatcggaa aaaatgaccg tacactatat acagatgact cgctaactac
    56701 cccacttgca actataaacg gcggagagct ggcggagttt ctttctgaaa caaaaaatgc
    56761 ttatcagatc aagacaaatg atggctatac tggatattta gccctcgaag atggaaccaa
    56821 agtcacaaag aatatacaaa caaaaccgga agagctatct gatgcagtga ttgtattaga
    56881 tcctgggcat ggcggaaatg atactggggc attaagcaat gatgaacaaa cagaagaaaa
    56941 agatataaca ttaagtacag ccataaaagt aaaaaaggcg ctagaggatg ctggagcaac
    57001 ggtttatttg acccatacta cagatgaatt agtacagctt ggagatatat gcgactacag
    57061 tgaagaaaaa aaggcagatg tctttatcag tttacacgca gactctacgg aatatgcgaa
    57121 tgaagcaact ggaatcacga catattatta ctatggacaa gaggaaacat tagcacagac
    57181 aattgctgac tccttcactg atctgcctct ggattcaaga ggaatctcca ctgggaacta
    57241 tcaagttctt cgagaaaatc ttcaaccttc tattttaatc gagatgggtt atatgaataa
    57301 tgatagtgat ttaaaggaat tagtaacaga cagttatcag cagcagattg ctgcaagttt
    57361 gacacaagca ttgaccactt attttcagta ataaataagc ccagccaaac aaaaatagct
    57421 cctcatattt ttcgtttggc cgggcttatt atcgcagatg caagctttta aacaccgttt
    57481 aaggaagagg tcgtgagcct gccgttcaac tctgagtcac aaggagcaat ttcaaaaaac
    57541 agcttctcat gtttgatgac tattgtgact tgtgaccgag gagttggcat gagaacaccg
    57601 tttaagaaaa aggttgtgaa atcaacgttt tgttctgagt attagagaaa caaacggaaa
    57661 aatagttcct catattttgc cgtttgttga gctaataccg aggatcagat gattgaacac
    57721 cgtttattcg gaacaaggga ctgtgacatg acttttgtca cagttccatt atttttttag
    57781 tagcttcaaa gctttttctg tattggcaaa atgaagtttg gcatattttt caagcatagg
    57841 catgccgcct ttctccaata gttttgccgc tacttggtca gatttcgttc cagctcctga
    57901 cggaagggtg attccagctt cagctgatgc tttgtctaac tcttccaaaa agctggcacg
    57961 actgatgata gaagcagcag caacagctaa atgatattgt tctcctttag taacgaagta
    58021 cagtttttct tgtaattgat ttttttctaa acgcgcatat tttttaaaat ttgcttccgg
    58081 cgtaaactga tcgatcaaaa tcgcttctgg tttttctggc tcgatttttt ttagcagcaa
    58141 atggatcgct tgattgtgca aagcgacttt catccgaacg gcattgtatt ttggttggat
    58201 ctcattgtat ttttctggag ataagacaag cagtttatag gggatggttt tttttaagat
    58261 gtcagcaagc tggatgattt ggctatcttt taactctttt gaatctctaa caccaagttt
    58321 acgtaattct gcaagctgat cacgtttcac ataagcagca catactgtta gcggaccgaa
    58381 atagctgcca tttccgacct catcactacc taaaacggat aattgactga tattttttgg
    58441 caaagaagag gctgtttttg ctggcttggc ttgtttatcc tgtgcctccc cccatttatc
    58501 agcttctatt gctgcattcg taccttgaaa catcactttt ccggatgtat aagcggtaat
    58561 ggtcgtcccg tctttttttg cagcaaagct agtataagga atctgtttat ttaacaaata
    58621 agagcgatag tatgctttca tttgactaag ttggtcgttt gttgctttga taatgacgtt
    58681 gctcataaaa ttctcctcgt ttttatacat aaaactatac cgcaactcac gacttttcgt
    58741 tttctaagag attgcgagac atagcatttt tttaaagttt atcatataat agttaagtat
    58801 gtgaaaaata tttgtggaga acggtgaaag aaccaacttc atggtacact aaataaaact
    58861 ttcatcaaat aggaggaaat gattcgtatg gcgcacgaaa aaacacgcta caaagcggtg
    58921 attgccaacc aaacatatac tattatcgga cgggaaacca agcatcatat ggatatcgta
    58981 acgaagctga tcaatgaaca gttagcagaa ttgaaacaat tatcaccaca aatggataat
    59041 gaacaagcag caatcttgat ggctgtcaat gcgctttcgg atcaattgaa gaaacaagaa
    59101 agaatattgg agctggaaga agaaacagca gaattaaaga aaaaaatgat caaatttaca
    59161 gagcttgaaa atcgtgtgaa acgcattgaa gcaatcgaga acgaagctcg ggaagtattg
    59221 aaagaaaatg gtcaagcaga tcataccatt aaaaatcatg tggaagcaca acagatttta
    59281 aacgagaaac gaaaaggaca aattaaacaa aaatcttcta attaagatcc ttttatcaaa
    59341 ataagaaaaa tttaaaaaag ttagaagaga aaggaacgta gcatgctgag tttgctgatt
    59401 ttatttttat tgctaattgc gtttttttct ggagcacgtc gcgggtttgc tttgcaaatc
    59461 atattcgcga ttggatatgt attgtcattt attgctgccc agcattttta caaaccatta
    59521 gcaagtcatt tggaattata cattccatac ccagctgtca ccccaacatc aaagttagca
    59581 ttttttgatc aggtatttgc ctttcaccta gatgaagctt tttatgcagg aactgctttt
    59641 ctgattattt tgctaattgg ctggctgctt acccgatttg tgggggtttt tgtccatgga
    59701 ttgacgtatg tgccgatctt gagacaggta gactggattg caggaggcat cctgtcacta
    59761 atcatggctt acgttactat atttttgatt ttgcagttac ttgcctttgt tccattagat
    59821 gtggtccaaa atcaattcgg cgagcatagt ttcgctcgtt ttatcgttga gagaacgcca
    59881 tttcttacca acaagatgta tgagctttgg gtaacgaata ttttgaatta aggaaaatag
    59941 tgctggaaaa tatttcgttt tttgtacaga gtagtttatt taagaaggct gcgtagcgta
    60001 tgtccggcac agctcttttt tcatagtgaa agataaggtg ataaaatgaa taaccgaatc
    60061 cttgaaacat tggaatttga aaaagtcaaa cagatggtca gacaatttgt agtgactgca
    60121 caaggaaaag aagaactggc agaattggta ccagtgagtg agaaacaaac gatcacaaac
    60181 tggcttcaag aaactgagga tggattgaaa gtgcaacgtc tgcgaggcgg gatccctata
    60241 cctaaaattg aaaatatccg tccccatatg aaacggattg agatcggagc agacttgaat
    60301 ggagtggagc ttgcacaagt ttccagagta ttgtctacga caagcgaatt gaatcgtttc
    60361 attgatgatc tttctgacag tgaaatcgaa tttgcacgtt tgtatatgtg ggcagatcag
    60421 ttagtcacga tccctgtttt gagccgccgt ttaaaggaag caatcgatga agacggacgc
    60481 gtgactgatg acgcttctcc tgagttaaag agcataagac agaacattcg gcgaagtgaa
    60541 caagctgttc gtgaacagtt agatgggatc gtccgtggga aaaatgcaaa gtatttaagt
    60601 gatgcgatca tcaccatgcg aaatgaccgc tacgttatcc cagtcaaaca agaataccgc
    60661 ggtgtatttg gcggtgtagt ccacgatcaa agtgcttcag gtcagacttt atttattgaa
    60721 ccaaaacaag tggtggattt gaataatcgt ttgcgtcaat accaaatcgc tgaacgaaat
    60781 gagattcaac gtattttaag tgaactttct gctgaacttg ttccccatcg tcaagaaatc
    60841 atccacaata cttatgtgat tggtaaaatg gatctgatga atgcaaaagc acgatttggt
    60901 aaagaagtaa aagccatcgt gccgggaatc agtgaagaca atcatgtcgt cttgaaacaa
    60961 gcaagacatc cactgatcga tcaggaaaaa gtagtttcta atgacatcac aatcggaaaa
    61021 gactatcaag ccatcgtcat tacaggacct aacacaggtg ggaaaacgat cacgttgaag
    61081 actttaggtc tgttacaact gatggggcaa gcaggattac cgattcctgc aggtgaagaa
    61141 agtcaaatcg gtatttttga ggaagtattt gcggatatag gagacgaaca gtcgattgag
    61201 caaagtctat cgaccttttc ttctcatatg acgaacactg tagatatttt atcaaaagtc
    61261 aatgaaaaaa gtctggtatt atttgacgag ctcggtgcag gaacagatcc gcaagaaggt
    61321 gctgctctag caatcgcaat cttagatgat cttggtaaaa agtctgctta tgtgatggca
    61381 acaacgcatt atcctgaatt gaaagtttat ggatataatc gggcaaatac gattaatgcc
    61441 agcatggagt ttgatgtgga tacattaagt ccaacgtatc gtttgctgat tggcgttcct
    61501 ggacgaagca atgcgtttga aatctctagc cgattaggtc ttgatacaga agttattgat
    61561 gaagcgaagc agttgatgaa tgatgagagc caagatttaa acgaaatgat cacagattta
    61621 gagaatcgcc ggaaaatggc agagaccgaa tatctggaaa tgcgtcattt tgtttctgag
    61681 gcacaagaac ttcacgatga tttgaaagaa gcttacagct atttctttga agaacgtgaa
    61741 aaagaaatgg aaaaagcaaa gaaaaaagca aatgaagtcg tgtcagaggc ggaagaaaaa
    61801 gcagaaaaaa tcattgctga tattcgtaaa atgcaacagc agattgggca aggaaatgtg
    61861 aaagagcatc agttgatcga tgcaaagacc caattagcaa atctacatca agaagaaact
    61921 ttgaagaaaa ataaagtatt gaagaaggcg aaagaacaaa aaacgttgaa accgggagac
    61981 gaagtgttgg ttacgactta cggccaaaga gggacgcttt tacgtaaaaa cgggaatcag
    62041 tggcaagtag aaatcggtat attaaaaatg aatgtgtccg aagacgagct gacgcctgtt
    62101 gctcctcaaa aagagccgac acaacgagtt atccatgctg tacgatctga atctagtagt
    62161 cacgtgccga atcaattgga ccttcgagga aaacgatacg aagaagcgct aagtgaggta
    62221 gatcaatatt tggattctgc cattctagct ggttatcctc aagtgactat tgtccacgga
    62281 aaaggaacag gcgcacttcg caagggaatt actgattatc ttaaaaacca tcggagcgtc
    62341 aaaagctttg agtttgcacc ggcaaaccaa ggcggaaatg gtgcgacaat tgttaaattt
    62401 aagtaaagct tactaaaggt aagcagatgg ttcgctatcc aagtatagaa atgctatact
    62461 agtcatagat tttaaaaatg gaggtctttg atatgtcaca agtaattaca gataagacat
    62521 ttaatgaaga aacagataaa ggacttgtat tgattgattt ttgggcaact tggtgtggtc
    62581 catgtcgtat gcaagcaccg attttggatc agttggaaca agaatatgat gaagaagaat
    62641 tcagaattgc taaaatggat gtggatgaaa atccagaaac tcctcaacaa ttcggtatca
    62701 tgagcattcc aacattaatg ttgaaaaaag atggtcaagt agtagaaaaa gctgttggtg
    62761 ttcattcaaa agaacaacta cgtcaaatga tcgatcaata tttataaaaa cttacagcaa
    62821 aaataagctg agggtgggac tgaatccagt tccgtcctcg gtttttttgc ggttgagtta
    62881 aggttattca aattaatatt ttttgagtat aattacgttg tcaggctgga ttgttttttt
    62941 gacagacatg ctataataac tttaattgaa tatgaagggg agtaccagat gaacgaacga
    63001 atcaaaaaca aattagcttt acttcctgat cagcccggct gttacttgat gaaagataaa
    63061 aatggaacga tcatttatgt cggcaaagcc aaaatattaa aaaatcgtgt ccgctcatat
    63121 tttactggta gccataatac gaaaacggaa cggcttgtta gtgaaatcga agactttgaa
    63181 tacatcgtta cagaatcgaa tatcgaagca ttgctcttag agatcaatct gataaagaaa
    63241 aatgatccta aatacaacat tatgttaaag gatgataaaa cctatccttt ccttaagata
    63301 acgaatgaaa aatatccccg tttggtgatt actcgtaagg tactaaaaga caaagcactg
    63361 tattttggac catatcccga tgtcggtgcg gcgaatgaga caaagaagat cctcgatcga
    63421 ttatttcctc tgagaaaatg caaacctagt cagacgaagg agccttgtct ttactatcat
    63481 ttgggacaat gcctgtgtcc ttattatttc gacgtagatc cgaaagtcta tacgaaaatc
    63541 gttgatgaag tgaaaaactt tttaaacgga ggacacgaaa aaatcgagtc agagatccat
    63601 cagaaaatgg aaaaagctgc tgaaaacatg gagtttgaaa aagctgcaga ataccgagac
    63661 cagatccgtg ccattgagac aatcatgact cgccaaaaaa tgacaaatac tgatttactg
    63721 gatcgagatg tatttggtta tgcgattgat aaaggatgga tgtgtgtcca agtgtttttc
    63781 gtaagacaag gaaagctaat tgaacgagat gtttcaatgt ttcctttcta taacgaagca
    63841 gaagaagatt tcttgactta cctcggtcag ttttatcagg aaaatgagca tttcattcct
    63901 aaagaagtct tgattcctga tacgatcgac aagcccagtg tagaagcttt gttagctacg
    63961 aaagtgctgc aaccaaaacg aggagaaaag aaaaaattag ttgaattggc gagtaagaat
    64021 gcctcagttg ctttgaatga acggtttgat ctgattgcca gaaaacagga acggacgatc
    64081 ggagcagttg aacgtttagg aaacgcgatg aatatcccga ccccaatacg tatcgagtct
    64141 tttgataact ctaatatcat ggggacgaat cctgtggctg cgatggtcgt attcattgat
    64201 ggaaaaccgg caagaaaaga atatcggaaa tataaaataa aaacagtcaa cggaccggat
    64261 gattatgcat caatgagaga agtcatctat cgcagatatt cgcgagtgat tcgcgaaggg
    64321 ttgcctttac ctgatttgat cttgatcgat ggaggaaaag gacaagtaga tgtggcaaaa
    64381 gatgtcttag aaaatcaatt aggaatcgat attcctattg ctggcatggt aaaaaacgat
    64441 aaacataaaa caaatgagtt gctattcggt acagatttgg ctgtagtacc gcttgagcga
    64501 aattcaccag agtttttctt actccagcga atccaagatg aagtccatcg attcgcaatc
    64561 actttccatc ggcagctacg cagtaaaaac agttttgctt cccgattaga tgaaattgaa
    64621 gggttaggac caaaacgaaa aaaaatgctg cttaaagagt tcaaatcgtt gaaaaatatc
    64681 acagcagcca cgacagaaga attacaagaa atcggactgc cgaaaaatgt cgcacaaaac
    64741 gtatttgata aattacatca ggaatgagga aagggcactt tacaaagaaa gtaaagtgtc
    64801 ctatcatttt atgtacaaca aaaagagcgt ttacgttttg ttggtaaata ataaatcgct
    64861 tacgtctggt gtctaaggat atatgtgatt tcttgaagaa ggagtgaaga aatgttagaa
    64921 gtcaaggatc ttgttaaaac ttttgggaac tacacagctg tcgatcatgt ctctttccaa
    64981 atccccgatg gaaaaatcat ggggctaatt ggccaaaacg gggctgggaa gacaacgact
    65041 tttcgtttga tcttggattt tttgacacaa gaccaaggag aagtgttatg gaatggccat
    65101 catcttgggg agaaagatta tgatgtcatc ggttatcttc cggaggaacg gggactttat
    65161 ccgaaagtaa cgattcaaga ccaactgctt tattttgcag aacttagagg gaaatcaaga
    65221 aaagagattg aaccaaaaat cgacttttgg atggaaaaat ttcaggtaaa agggaaaaag
    65281 acggataaag taaaatcttt gtctaaagga aatcagcaaa aagtccagct gatcgccacg
    65341 ttaatccatg aacctaaatt gatcattttg gatgagccat tcagcgggtt agatccggta
    65401 aatgctgaat tgctaaaaga tggaatattg gaattgaaaa aacatggttc atgtgtcatt
    65461 ttttctagcc acaatatgga taatgtagaa aaaatctgcg atcatttgat catgttgcga
    65521 aacggaaaaa tggttttgaa tggaaaagta catgagattc gtgaatcatt tggccgtacg
    65581 aagttattct tggaatctgg tttaagccaa caggaagtag aagaaatcga aggcgtgaaa
    65641 aaagtgatcc ttcatgaaga caatacgtta gagattacct tagaagatcc tgaagctggg
    65701 aaagaaatct ttactcgtgc gactcagttc ggctacattc ctatgtttaa ccagcaaccg
    65761 ccaactttgg aagagatttt caaattgaaa gcaggtgaaa tcgatgcgta agttttggat
    65821 tattaccaaa gatgtctatt taaaaaatgt taagtcgatt tctttcttga tcatgatatt
    65881 agtccctttt atcatgctgg gtatcattta tgtagcagga aactttgctc aacaaaatag
    65941 tgatgtcaat aaaatcggcg tattggctga taatgagcaa atcagtcagc aaatgagcca
    66001 agtcaaaaca gatgattttg agttcaaagc agttgattct gaagaaaaag cacaaaaaga
    66061 gctttcggaa gaaaaaatcg atgcgtattt agtagtctca actgagaatg ataagataaa
    66121 tgggatgctt tatagcgaaa attcattagg acaaagtaca caactactga tacaacaaca
    66181 actgactggc ttgcaatcga tgcttcgagc gagtcaatta ggcatttcac cagaagaagt
    66241 agctagcttg agccaacaag caagtttgac tcgacaaaaa gtcaattttg attcaaaagg
    66301 aaaaatgaca gtcggagaag ataactcgac agtccaatac gtcgtaagtt atgtagcaac
    66361 gattgtcttg ttcattatta ttttgacgta tgcgcagatc attgcacaag agatcgcttc
    66421 tgaaaaaggc acgcggatca tggaagtgat cctgtccagt acgactgcac aaaagcattt
    66481 ttacggaaaa ttaacaggtg ttttacttgt tgcattaacg caaatggcat tatacggtat
    66541 tatttttggt ataggataca gtcaattcaa agatatggaa atggtcaaaa atatattaga
    66601 cggtatctca ttggataact tgattggacc attcttgtgg tattctcttc tattcatgat
    66661 ctttggcatt ttgatttttt ctgttttagc agctttatgt ggctctttgg tcaacaaagc
    66721 agaagataca gcaaaagcaa ttttgccagt gacttatcta tctctaggcg gatacatgct
    66781 tggattgata ctaggagctt cagatccaaa caatatcgtt attcggatca cttcgtatat
    66841 tccattctta tcttcatata ttatgccgat tcgcttagca aatgagacag tcggtatggg
    66901 tggagcattg atttccttgt tggtacttgt agccgtaaca ttcttattga tgatcttgtc
    66961 tgcaaacatg tacaaatcga atgtactcgt ttatagcgaa ggcggattat ggagttcatt
    67021 gaaacaatcg atttcaatta tgaggaatga acgaaaaaaa gcataaaaca atcctaaaat
    67081 acagaaggaa gctggacatc tattgttccc agcttctttt tgtatagacc tattttattt
    67141 attttactat attggatata tattcattga aaatagtgta taacaacttt ataatattga
    67201 gtataccaat ttttggaggc gttttaatgg accgttattt agatttactt tcagaaaaat
    67261 tcccctcaac ggaggatgtc atcacggaaa tcatcaatct cgaagcaatt ttgcagcttc
    67321 caaaaggaac agagttattt ttgagtgata tacatggcga atttcctgct tttgatcata
    67381 ttctgcgtat cggttcagga aatcttaaag aaaagatcaa agaccttttt tctgaacaaa
    67441 tgacggaaac ggatatcagc caattcacta tttttacggc ttatccagaa tatgcattaa
    67501 cgactgattg gtacaaagaa caagataaat cgcagctgat ccatcatttg atcgatcttt
    67561 tacggtttac gacagtcaaa tatacccggt caaaagttcg aaaaggattg ccgaaagaat
    67621 atagctatat cattgaggaa ctcttgtata tcgatgaccg tatcaatgga aaaaaagact
    67681 atgtaaaaaa aatcattgaa caacttgttt tgatgaagga agaagaaaaa tttttaacga
    67741 aacttgcaca gacgatccaa aaatctgtca tcgatcattt gcatatcgtt ggagatattt
    67801 ttgaccgagg aactcaagca gccaaagtga tggatcgaat catggatttt tcttctgtag
    67861 atatccaatg gggaaatcat gatattttgt ggattggtgc gtattgcggc tctgaggcat
    67921 gtttactgac acttttacgg attgcagcac gctataatta tttatttgaa ttagagaaag
    67981 aatatgggtt gaatttacgt ccattgtttt cttttgctca tcaaacttat cagaagaatc
    68041 cagtatttgc gcctaaaaac agtaaaaatc tatccgaaag agagcaagaa gaattagaaa
    68101 aaatccatca ggcactttct atccttcaat tcaaatcaga acatcagtta ttggatcgtc
    68161 gacctgaatt taaaatggac gatcgtaaat tgctggaaaa aatcgactat gaagcatcaa
    68221 cgattgattt agaaggtcag ctctatgagt tgaaaggaac ttgttttcaa acgatccagc
    68281 cagatgatcc aaaaaaattc ttggaagagg aacaaaaagt gattgatagt ttgatgtatt
    68341 cattccagca ctctttgaga atgcaaaaac atatgacgtt tttgatcgaa aaagggggaa
    68401 tgtatctcac gaacaatcag catgtgctgt ttcatggctg tatacctgta gacgagaagg
    68461 gacagctctt agcatttgaa ttagaccaat cttatcgcgg aaaagaactg ctgggctttt
    68521 ttgaaaaaca gataactgaa agcgcaaaag atctgactgc aaaagaagat ttagcaaccg
    68581 atttgatttg gtatgcgtgg agtgggccat tttcgccttt attcggcaag agtaaaatgg
    68641 cgacttttga acgttatttt ctcacagagc ctcatacgca cgtcgagaaa agcaatcctt
    68701 attatcactt gcgagatgaa gagtggttca gtgaaaaaat tttagcagaa ttcgacacaa
    68761 aagaagaagg gtcagcaatc attaatgggc acacgccagt aaaagtaaaa aaaggggagt
    68821 caccgatcaa agcaaatgga aaagtatttg tcattgacgg aggtttatcc aaagcttatc
    68881 aaaaaacgac aggaattgcc ggttattcac tattatgcaa ttcttatggt ttccaaatcg
    68941 tgacacatta tccgtttttg cctattgaac aacaatttgc aaaaaaatcc gaccaaacaa
    69001 atgtgaaaaa agtaatcgaa cagcaactac cacgcaaact caatcgtgat acaacaaaag
    69061 gtatggaact gcagcagcag attacccagc tcaaaaggct gttggactat cgaaaggacg
    69121 attgagaaga attccagtga acattgttcc atagactgga gactgagaca aaagtcgtgt
    69181 cgcagtctct ttttccggat aaacggtgtt cttctgccaa ctcctcggtc acaagtcaca
    69241 ctattcaaca aacatgaaaa gctgtttttt gaaattgctc cttgtgactc agagttgaac
    69301 ggcaggctca cgacctcttg gtataaacgg tgttcaaaag ccagtttctc ggtattaagc
    69361 caaatttatg caaaaatgtg aaaagccatt ttcacaaaat ttttcttaat actcgaaact
    69421 aaacagcttt ttcacaaccc cctcagaaag tctattaaaa ttcattcaca aagaaatcga
    69481 ataagcgctg ttcagatgga tgtacacggt gggtaagttc cggatgccat tgaacaccga
    69541 ggattttatg catatcatct ttgctttgaa ccgcttcaac gagtccatcg ttagaaaaag
    69601 cgatggcttt taataaaggt gaaagatctt tgactgcctg atgatggtaa gaattcacaa
    69661 aatatggatc ggggagaagg ttgcttaaca aactgtctga aacaatcttg acttcgtgag
    69721 tcgtaaattg cggggcagtg ggctgttgtt catgttttac tgaccacttt ggatataatg
    69781 aaagatcttg ataaagtgta ccttccaaaa cgacattcaa tagttggagt ccccggcaga
    69841 ctgcaaaaat tggttttctt tgtttcaatg cttccaagat cagtgcctgt tcaaattggt
    69901 ctcgttgaat atttgtttcc gttagctttg gatgaggatc ttccatgtaa agttgtggac
    69961 tgacatcttg accacctgca agaataagtt tgtcgatttg tccaatcagt tgaggagctg
    70021 aatctggaga catgactggc agaataagag gcaatccttg agcatctaaa actgcatcca
    70081 cgaatccttg cggtgtatag ctcacttgat ttccttggaa agtatctgtt gctcgaatca
    70141 gttgatttcc ggcaatgccg atgattggtt tcatttgtat tccccctaaa gtagacttat
    70201 tgtaacaagg atttaaaaaa aattaaacta tgtaagactt gcccctatac ttatctgttc
    70261 ttgctgtgtt actatctgta aagagaaatt tcgtgtgaaa ttcatgacga tagttgaaaa
    70321 tattgcttaa tttgttataa taaaaagtaa gcagaaataa aaaggttggg cgtaagcttc
    70381 aaggatctag ttcttcaagg ggtgagaagg acattttatt gaaggggcct gcctatacta
    70441 agttatcttt agaggtacat actttcaatg aaaaaagaaa gcatgtacct cttttttaaa
    70501 tcgaaaggaa gtgaagcaag tgttagaagc tttggaacaa atccgaaaag cagaagaaga
    70561 aaatgaaaag cgtgctcaaa cactgcggca gcagctgaag atctatgcaa cagaaaaaga
    70621 gcaggaattg gaaacgatcc aaacaaacta tcgacaacaa ctaaaacagt tggtggaaga
    70681 aaaagaacaa tttgaaatac aagaattaga aaaagaaaaa atgaaattga aagcagaagc
    70741 agacaaggtg caagcaactt tgaaagatca atatcaaaag catcagagtc aagcgatcgg
    70801 tgcgatcatt gaaagggtga aagaaactta tggccgtcac taagatggaa aaagtgaccc
    70861 tcatttcaga taaaagaaat caggagagga ttttacaagc agtacaagga atccaaaacg
    70921 ttgaaatccg ggatctgtat caaaatagaa caaacaacca gtgggtggag aagtattttc
    70981 cagataccac gattattgat aaagaagcaa aattaagcgc tttaggtaat cggttgaaag
    71041 acatccaaga agctatccaa ttcatacaac accacggcga tagccgtcaa aagaaactcc
    71101 atttgaaacg tcaagaactt tccttacaag atttggaagc caattattcc gaagaaaatt
    71161 tcataaataa attgaaagaa gtccttgatt taaaagaaaa gtgggaacaa ctaactgaag
    71221 aacgtgatcg tttagaagaa gaggaagaat ggcttcttca ttggcaacag ctggatattg
    71281 ctccaagtat gtatgcaagt aagacgacca tttttgagat ggggactgtg agtgcagcaa
    71341 attttgaaag tttcaaggaa gaactgagta agcttgatga cgtttatatg gaagaaatag
    71401 attatacgcc aaaacaagtt tatctttcgt atgttgcttt aaaaaaatca gttgctgtta
    71461 tagaagaaat tgctgggaaa tacgggttca ataaagaaac gtatccttat gaacaaaatc
    71521 ctaaagaaaa actgcaagag accaagcagc aattatccat tgttcacgag cagcaaaaga
    71581 agataacgac agcgttaggt cagtgcagtg gctatatcca agaatttgaa tgggtagaag
    71641 aagtcacact tgccgttgca gaacgagaaa aaataaaaga acggtttatc catgcgactt
    71701 atctgatcgt tttgcaaggt tgggtagatg cagaagaaaa acagaatttg ctgcatgtgt
    71761 taaacgaaac cgtatcagaa gaagatatat acgtcagttt cgaaacacca aactcagaag
    71821 agattcagca ggaagtccct actaaattga aaaaccatcc gcttgtcgaa ccttttgaat
    71881 tgctgactga aatgtacagt ctgccaaaat atgaagaagt agatccaaca ccttggatga
    71941 caccatttta tcttgtcttc ttcgggatga tggtcgcaga tgttggttat ggtttgctga
    72001 tgctcattgg gacaatacta gctcaaaaac tacttgttct accacgcggg atgaaacgtt
    72061 tcgtaaaatt ctttgagatt ctctcgatac catcgatcat ctggggattg atctacagtt
    72121 catttttcgg acagtcatta ccaaaagaac tgtttggcat ccgcttgcct ttcccaatcc
    72181 tatctacgac ggatgacgtc aatacgattt tgattttatc cgtgattttc ggtctgatac
    72241 agattttggt tggattattt gtggcagcaa aggaaaatat caggagaaaa gattacttga
    72301 gtgctgtcag tgatggattt gcctggcaag ggatcctaat tgggatcgtt attgcgttgg
    72361 ttggtgccat gttgataaaa aatacagcat ttgtttacct tggcgctggg ctatcgatca
    72421 ttgctgcctt atgtatcttg atcgttccga ttatccaaac gccgtctaaa gtcaaaggtg
    72481 ctgccaaagg tgcgtataat ctttacggac tgaccggcta tatcggtgat ttagtcagtt
    72541 atacacgttt gatggcattg ggtatttcag gcggaagtat tggagcggca ttcaatatgc
    72601 tagttgcttt catgccgcca gctgctcgat tcagtgtagg gatccttttg atcatcgctt
    72661 tacacgcact gaatatgttt ttgacactgc taagtgccta tgtccatggc gcacgattgc
    72721 agtatgtcga attttttggg aagttttata caggaggcgg acgtgccttt gatccgctga
    72781 aaacggcgga aaaatacgtc aacatcaatc acaagaaaaa aaacaataaa taattggagg
    72841 atttttatat aatgatggat tacttaatta gtcaaaatgg tggaatggta tttgcggtat
    72901 tagcgatggc aacagcgacg attttttcag ggatcggttc agctaaaggt gttggaatga
    72961 caggtgaggc agcagcagca ttgacaacaa gtcaacccga aaaattcggg caggctctga
    73021 ttctgcaatt gcttccaggc acgcaaggtt tgtatggatt cgttattgcc tttttgattt
    73081 ttatcaactt aggaaatgac atgtcagttg tacaaggact gaactttcta ggtgcttctc
    73141 tgccgattgc ttttacaggc ttgttttcag gtattgccca aggaaaagtc gcagcggcag
    73201 gtattcaaat tttagcgaaa aaacctgaac atgctacaaa aggaatcatt tttgctgcga
    73261 tggttgaaac atacgcgatt ttaggtttcg ttatttcctt cctattagtg ttgaatgcct
    73321 aagcataaag gaggaaaagc agatgaacgc cattgaaaaa atcatctctc agatgaacga
    73381 agcagcagag caggaaagag cggcgttaga acaagaagag cgaatgaaga tcgatcaaaa
    73441 ctttgaacaa aaacgtacgc aagttgaaac agaacatcaa aaacaaaaag aaaaacaaat
    73501 cgaattactc gaaaaaaaat accgtcagct aaggaatcgc cagcaagtag aagtacgaca
    73561 agaaaatttg aatgcgaagc aggaattttt aagacgattg tttgcagatg cggtaactga
    73621 aatggaaaat tgggatgaat ctgaacaaat ccagtttatc aaaaatgcat tgtattcatt
    73681 acctttgaca ggaaaagtag cattcattgc tggagaaaaa tcagcagcct atctttctca
    73741 gacgttgttg gatgaatgga acaatgaact tccctttatg atggttttaa gtgatgaaac
    73801 tgttgctgat caagcgggtt ttctgataaa cgatcaagga gttcagtata attttctttt
    73861 cagcagcctt gtacaagata tccaaggaac gatgagtttt gagattgcca atcaattatt
    73921 cgaataagga gggatcagtg tatggaatac cacgaactaa atcccttgat tcgcggaaga
    73981 gagcttgaac ttttgtccaa tgagacattt gaacggatga ttcaaacaaa ttctgtcgaa
    74041 gcacttggtg aaatattgaa atccacgatt tacagtccat atatttatga cggatatgaa
    74101 tatgacttcg aacagaatct tttaaaggag cagagccgtc tatttcgctg gttaaaggaa
    74161 tcagcaccgg agcctgaaat cgtttggatc tatacgatgc gttatacttt ccataatctg
    74221 aaggttttga caaaagctga gatgacaaat caaaatctgg accatttgta tatcgacgat
    74281 ggattttatt cattagaaac cttgaaggat gccattcata cgcaagtttc aaatgaactt
    74341 cctgaaagca ttatggacag tatacgagaa gtacgtgatt attttgaaga gtctaccatt
    74401 cttcagggaa ttgacgtgat ttatgatcga cagtttttga cagagcagcg acggcttgcc
    74461 gataagctag gatatcaaga attgctgaca gaagtcattg cttttattga cttgaccaat
    74521 atcacaacga tggctcgagg aatattgcag aaaagaacat atgggttcat gacgacggta
    74581 ctttccagct cgggaagtat cgaaaaagaa gtatttttga actttgtaga taaagatatg
    74641 gaagattaca cgaattttct attagagaca gattatgctg attttctgcg ccctgtcatc
    74701 cgtgagggaa atattgatct cgtaaagctt gaacagttga aagatgatta tctaagttca
    74761 ttgtatcaag aagcacaaac ccaggctttc ggaccactgc cactactagc ctttttgaac
    74821 gcaaaagagg tagaaagcaa aaaccttcgt ttactggtta tcggcaaaag aagccatttc
    74881 gaatcagaaa caatcagaaa gcgggtgaga caagtctatg acgcataaga tcggtgtagt
    74941 tggcgacaaa gattctgttt tgccattcaa attgtttggc tttgatgttc gttatggtac
    75001 aaccaagcaa accgttcgtc aggcaattga agaaatggca aagaatgagt atggcgtgat
    75061 ttatgtcaca gagcaatgtg cagcattgag cccagaaacg atcgaacgtt acaaggaaca
    75121 aatgatacct gctatcgtat tgattcccaa tcatcaagga tcattaggga tagggctgga
    75181 agaaattcaa aaaaacgtag aaaaagcagt aggtcaaaat atcttatagt atgaaaagga
    75241 gatagaaatt ttgcaaattg gaaaaatcat aaaagtctcc ggacctcttg taatggctga
    75301 gaacatgtct gatgccagta tccaagacat gtgtctagtg ggagatctag gagttatcgg
    75361 agaaattatc gagatgcgtg gcgatgtggc gtctattcaa gtatatgaag agacttctgg
    75421 aattggtcca ggcgaaccgg ttcgctctac tggtgaagct ttatctgttg aattaggacc
    75481 agggatcatt tcacaaatgt ttgacggaat ccaacggcca ttagatacct ttatggaaat
    75541 cacacaaagt aatttcttag ggcgcggagt tcaattgccc gcattggacc atgagaaaaa
    75601 atggtggttc gaaccaacag tagaagccgg agaaacggtg agtgctggag atgttatcgg
    75661 gatcgtagaa gaaacaaaag ttataaaaca taaaatcatg gtgccaaatg ggataaaagg
    75721 aacgatcaaa acaatcgaaa aaggttcatt cactattgat gaaacagtat gtgtcattga
    75781 aaccgaaaat ggtgacaaaa atttgacgat gcttcaaaaa tggccagtaa gacgtagtcg
    75841 gccaatcaaa gaaaaattaa acccggatgc acccatgatc actggacagc gagtgattga
    75901 tacattcttc ccagtgacaa aaggaggagc agcagctgta cctggtcctt ttggtgcagg
    75961 gaaaacagtc gttcagcatc aaattgcaaa atgggccgat gtggacatgg ttgtctatgt
    76021 gggatgtggc gagcgtggaa atgagatgac ggatgtttta aatgaattcc ctgaattgat
    76081 tgaccctaat acaggagaat cgttgatgga acgtacggta ttgattgcta atacatcaaa
    76141 catgccagta gcagctcgag aagcttctat ctatacggga atcacaattg cggaatattt
    76201 ccgtgacatg ggttatgacg ttgctatcat ggcagactcc acttcacgat gggcagaagc
    76261 acttcgtgag atgagcggac gtttggaaga aatgcctggt gatgaaggtt atccagctta
    76321 tcttggttca cggcttgcgg aatactatga acgatctgga agagtcattg cattaggatc
    76381 agaaggtcgc gaaggaagta taaccgcaat cagtgctgta tccccgtctg gaggggatat
    76441 ttcggaacca gtcacacaaa atacattgcg ggtcgtaaaa gtattctggg gtcttgattc
    76501 cagtttagcc caaaaaagac attttccttc gatcgattgg atacaaagct attctcttta
    76561 ttcaacagaa gtagggaagt atatggatca tatccttcaa gctgactggg catccatggt
    76621 caccgagggc atgcgtattt tgcaggaaga acagcagctg aatgaaatcg tgcgtttggt
    76681 agggattgat tccctttcag ataatgaccg tctgacatta gaagtggcaa aatccattcg
    76741 tgaagattat ttgcagcaaa atgcttttga tgatgtcgat acattcactt caagagagaa
    76801 acaattcaaa atgttgaaga tgatcttgac ctttggtgaa gaaggacgcc gtgcgttgtc
    76861 gttaggtgct tatttaagtg agatcatgga cggtaccgta tcgattcgtg aaaggatcgg
    76921 acgaagcaaa tatatcccgg aaacagaatt agaaaaacta gatcaaatta ttggtgaaat
    76981 caagcaaacg atgcaaacga tcgtttcaga aggaggcatg agcaatgatt aaagaatatc
    77041 gtacgatcgg tgaagtcgtc ggtccattga tggcggttga aaaagttgct ggtgtcaaat
    77101 atgaagaatt aatcgaagtc cgtatgcaaa atggcgagat tcgtcgtgga caagtattgg
    77161 aagtccaaga agataaagca atggtccaaa tattcgaagg aacaagtggt atcaatttac
    77221 gtgactcttc tgtacgattt ttaggccatc ctttagaact aggtgtatcg gaagacatga
    77281 tcggtcgagt attcgatggt ttgggacgac cgaaagataa cggaccagag atcctgcctg
    77341 aaaagcattt ggatattaac ggagaagtaa tcaatccagt agctcgggat tatccagatg
    77401 agtttatcca aacaggtatc tctgcaatcg atcatttgaa tacattagtt cgtgggcaaa
    77461 aattaccagt attttctggc tctggtcttc ctcataaaga attagctgct cagatcgcgc
    77521 gacaagcaac ggtactcgat tcatcagatg atttcgccgt agtctttgct gctattggaa
    77581 tcacttttga agaagctgaa tttttcatgg aagattttcg tcaaacgggc gcaatcgacc
    77641 gttctgtcat gtttatgaat ttagcaaacg accctgcaat cgaacgtatc gctactcctc
    77701 gtatggcttt gactgctgca gaatatcttg cttatgaaaa gggaatgcat gttttggtta
    77761 tcatgacaga catgaccaac tatgcagagg cattgcgaga gatttctgca gcacggcgag
    77821 aagttccagg aagaagagga tatcctgggt atctttacac gaacttagct actttattcg
    77881 aacgagcagg aagaatccgc ggactaaaag gatcggttac tcaaattccg attctaacaa
    77941 tgccagaaga cgataaaact catccaatcc ctgacttgac tggttatatt acagaaggac
    78001 agatcatctt gactagagag ctttacaaga gtgggattca gccaccaatt gatgtactcc
    78061 cgtctctttc gcggttaaaa gataagggaa ctggacccgg aaagacaaga gaagaccatg
    78121 cagctacgat gaatcagctt tttgcagcct atgcacaagg aaaacaggca aaagaattag
    78181 ccgtagtttt aggtgaatct gctttatctg atgtagacaa gatctatgcg aagtttgctg
    78241 agcggtttga aaaagaatat gtcaatcaag gattctacac gaaccgttcg attactgaaa
    78301 cacttgatct agggtgggaa ctgctatcga tgttgccaag aaccgaatta aaacgaatca
    78361 aagacgacat gctggacaaa tatctgccag aagggaagtg agttctgatg gcacgattga
    78421 atgtgaatcc gacccgcatg gaattgactc gattgaaaaa acagttgaca actgccacaa
    78481 gagggcataa attattaaaa gataagcaag atgaactgat gcgtcaattc atcctgttga
    78541 ttcgaaagaa caaccagctg cgtcaagaaa tggaagagtc tatgacagaa gcaatgtctg
    78601 attttgtatt ggcaaatgct tcgatcacgg aacctttcat cgaagaatta tttgttttgc
    78661 cggcggaaaa cgttgatttg tctatcgtag acaaaaatat catgagtgta aaagtacctg
    78721 tgatgaattt tgaatatgat gaacagctga cagacacacc tttggaatac gggtatttga
    78781 attccagtgc cgaactggat cggtcaatcg atcggtttac taccctgctt ccaaaacttt
    78841 tggaactgac agaaatcgaa aaaacttgtc aactgatggc gagtgaaatc gaaaagacac
    78901 gtcggcgagt caatgcatta gaatatatga cgatccctca attagaagaa acgatctatt
    78961 atattcgtat gaagctggaa gaaaacgagc gcgcagaagt aacaaggttg atcaaagtaa
    79021 aaaatatggg taccactgac taagtggtat ctttttttaa aagaggaaaa cgatgattaa
    79081 acaaagcaaa caaataaaga aacgtttgcc ggctgttcag cttctcgcat taggattttt
    79141 tctcctgatt ttagttggcg gcggcctact taccctacca ttttttagta atagtgggca
    79201 tgggacaaat tttatcgatg cgcttttcac tgcaacttct gctgtctgtg taacgggatt
    79261 gacaacattg aatacagcag agcattggaa cagtgctggt caatttttga tcatgctttt
    79321 gattgagatc ggcggtcttg gttttatgat ggttccgatt ctatgctttg cattgataaa
    79381 gaagaaagtc agtctcagca cacgtattat attaaaagaa gcgctgaatc tagaagaagt
    79441 atcaggtgtg atgaagctca tgctttacat cctaaaattg gcctttgtga tccaattgat
    79501 tggtacggtt gctttatctt ttgtatttgt gcctgaattt ggctggttga aaggtatctg
    79561 gtacagtttg ttccatgcaa tctctagttt ttgtaatgca gggtttgatt tgttaggaga
    79621 cagtttagcc aatcaccagc aaaatgttta tttgatcatg gtggtatctg ctttgatcat
    79681 ttctggtggg ttaggcttca tcgtatggcg tgatttactg gaatatcatc agaaaaagaa
    79741 gatgacgatt cattcaaaaa tcgcattaac cgttacaggt acccttttat taggcggatt
    79801 cgttgtgttc tttttcacag agcgcaatgg ggcaacgcta gtggaaggga atatgtttca
    79861 acgattggca aacacctttt ttatgagtgt gacacctaga acggctggat attattcagt
    79921 tgattacctc catatgagcc atgcaggtct gattttaacg atgtttttga tgtacatcgg
    79981 cggaacatct gggtcgacgg ccggcggatt gaaaacaact acgttaggtg tgttgctgat
    80041 ccagatgcgt tcgatgttga aaggacgtac gagagcggaa gcatttggcc gtaccatccg
    80101 acaaggcgca gtgttgcggg cacttacttt attttttgtt acgttgacgt tgtgtgtagt
    80161 cgccatcatg attttatcgg tgactgaaac gatacctaaa acttctggca tcgaatatat
    80221 tgcttttgag gtcttctctg cgtttggtac agtaggtctt acgatgggat tgacgccaga
    80281 cttgactgca atagggaaat tgatcattat agcgcttatg tacattggac gagttggtgt
    80341 attgactgtg gtcttctctc tgatgacaaa agcacaaaga acacaatcaa aatacaaata
    80401 tccagaagaa agtgtcatga ttggataatt tcatcttctg acagaaacct agtgatttag
    80461 taaaataaga aaataaaata atttttcttg cttcttggat actttaagtg caaaatgttc
    80521 gtttattttt tgaggcacta cccgttgttg aacctcgggt agtgcctctt ttttattgaa
    80581 aggaatcaat aagatttgat tcctttattt atcaataggt tataattcat atttttttat
    80641 tttttctttt acgatactta gtccagaagc taaaatttca aggttattag aggataggga
    80701 aattcgaacg aatgcatctg gttgcgcacg tgtagtaaag cgtctggaat gataaagtcg
    80761 gataccgtta gcaagaaagt cattctcaat tatcttaggg tcaatctcag gatcaactgg
    80821 aagaattcga tagtaaggga cacgattcgt tggtttttct actttgaaaa gagaatcaaa
    80881 aagctgattg gctctttttg taagacttaa tttagtttct aagatcctat ttgcttcatt
    80941 tgaatggagg atttgagtga tgacttccac atctaatcca gaagttttca catttatatt
    81001 aaaaattgct tgttctatct tgctttggac atgtactgga actacaagat aagcaactcg
    81061 caatccagaa caaataaatt ttgtcattcc agctaagtag attgtttggt taggtaataa
    81121 attttggaaa ggaggaatga tcgtatgttg atagtaagtt gttaaaaatg aatggatatc
    81181 atcttcaata acccatagac cttctttttc aatcacttcg gctaattgct ctcgtctttt
    81241 gtgagagatt tgaaacccta taggattatt acaagaaggc ataagaaaga caccatgaat
    81301 cttctttttt ctacattcct gaatcaatat atcaggtcgc attccttgct cgtcaaaatc
    81361 aattggaacg atctctaaat gatataactg agccaattca atgaaattag cgtatgtata
    81421 tcggtctgtt gctattctat cacctggaga gaaaagtgca gaaagtgtga tagccaatcc
    81481 attttgaaca ccggaaacaa tagatagaga ttctaaggac ggaatgactc cttgagtttt
    81541 taaccaattt gctgcagtag tcaattgaaa atttgtgcct ttgggatctt ggtaattaag
    81601 caaaccaaca agatttgggt tattactagt tgattgaata aacgggacaa cgaatttatt
    81661 acattcttca aaagaactaa ccagaccaaa atcaatgata tgttcggtta cttcattggt
    81721 agagatggtg atggactcaa agacattggg agaaataaat gtgccacttc caatattcgt
    81781 atacagcaaa cctttctcaa ttccatattt ataagccttg ccaacggtag taaaatttaa
    81841 atctaaataa tcagctagct ctctttggga gggtaactga gtgtttttct gtaatttatt
    81901 ctttaaaata tcttgttcta attgttgaat caacgatcga taataaggac gcttcaattt
    81961 ttgtttgtct ggtcgccaag ttaacgggta attgtcataa gaattaatag gcattttctc
    82021 cactccttaa aattgtatac catacaattt taccattgat tgtattgatt ttagaagtta
    82081 atcttttgtg aggagggaat tgtctatgaa aattattaat tttgaggaag tagtagcttc
    82141 ttttagtttt gaagaagcga ttcaagtaat gaaaaaatgt ttttttgatt atgaaagtgg
    82201 taaaatcagc caaaatcctc gattggtgga aattttacct gacggaaaag gcaaaaatat
    82261 gtttgcgatg atgcctgctt atttggggga gaacagatat tttggcacaa aagtcattac
    82321 cgcttttccg gataatcatc agtatcattt accttctcat ttaggagaga ttttcttgtt
    82381 tgatgctcaa aacggactac ccaaagcatt gataaacgca aatgcagtca cctggattcg
    82441 aacagctgct gtatctgcgc tggcaacaga ttacttagca aatccgcatg cagaaacact
    82501 tgctttaatt ggagctggtc aacaagcatt gtcgcatttg actgcgattt ctaccattcg
    82561 tcccatcact acagtgtttg tttacgatat tttagaagaa agacgaaata cttttatcgc
    82621 taaagctaaa aaacagtatc ctaaaataac gtttataaat gcaatatcag tagaagatgc
    82681 agtacgtcca gcggaaatca tttgtacgtt gacctctagt aaagaggcat ttcttcaaaa
    82741 agagtgggta agggaagacg cacatatcaa tgctgtgggc acatttactc ctgatacaag
    82801 agagataagt acatcgttga tggaaacttc tcaaatctat gtagatgatt actcagctgc
    82861 cttaacagaa agcggggacc tgcttatagc catggcagaa ggagatttag aggaatcttg
    82921 tattgttggt acattaggcg aattagtttc tgagaaaaaa acattttata aaggagaatc
    82981 gaggccgacg attttcgacg cagttggtct agctattgaa gatctttgtt gtgcagaata
    83041 tatttataac aaattagaga aaggggcttt aaaatgaact tagattatta cgtagttgat
    83101 gcatttgcag atgaggtgtt caaaggaaat ccagcagcgg tttatgtgct agaagaatgg
    83161 ctgccagaag gtacgatgca aaaaattgcc attgaaaaca atttatctga aacagcattt
    83221 acagtgaaga aaaaccaaga atttgagctt agatggttta cgccagatcg agaaatcgat
    83281 ctttgtggtc acgcgacatt agcaacagca tttgttttat ttaactatta caaaatacct
    83341 gatgaaacga ttaaattttc tactcaaagc ggtaatctat ttgtgacaag aattaaagat
    83401 tattattaca tggattttcc tagtattatg ccgaaaaaag tgccgatatt gtctgaatat
    83461 gaagaagcga ttggggctaa gattaaagaa gcctacttag cacgtgattt atttttcgta
    83521 ttagaagatg aaaaaacggt ggctaaattg cagccagatt ttacagctat caaaaatttt
    83581 gaattaggaa taggagtcat cgtgacagca gaaagtgtgc aaaaagactt tgtttctaga
    83641 accttctttc caaaattaac aatcaatgaa gatccagtat gtggatcagc acattctaat
    83701 cttattcctt attgggcaga aagaatgaat aaaacaaaat tgtcagctta tcaaatgtca
    83761 tcaaggggcg gggatctaga atgtgaatta aaagaagatc gtgtgataat aggaggaaaa
    83821 gcagttttgt tttctaaagg tacctcgttt atttgatttc tttcaaatga attaaaaata
    83881 aattggtgga gagtggcaac cttatagcta caatggttgt taaaaaaaga cgcaaacttt
    83941 ttcaatttaa tgaaaagttt gcgtctcatt ttaattattg gtgtttgttt tttattcaat
    84001 caagagtaaa atgccccata ctaactatcc tcggatgact tcgattttat aaccatcagg
    84061 gtcaatgaca aagtagtaag aaggcgcagt gccaggtaat ccttttaagt cagtaatctc
    84121 aagaccagca gctttatgtt gctcgtgtaa tttttctaga tcatcagctg ctatagcgat
    84181 atgaccatag ccgtctccta gatcatacgc accatgatca tagttgtaag tcaactctaa
    84241 ttcatagtca tccccaggaa gtgtcaaata aacaagtgta aatttgtttt ctggaaaatc
    84301 acggcggcgg ctttcttcaa aaccaaatgc cttttgataa aattcgattg atgcttcaag
    84361 atctttcact cgaacacatg tgtgagccat tttcatagat tcatctctcc ttttcctttt
    84421 actcaatcat aacaaaaaga agtaaggaaa tagttgattt gtgcttgttc atgagaaatt
    84481 tctcatgtaa aatggataga aaataaaact gttaaggtgg aaactattca tggataaacc
    84541 aatttttggt gaaaaaaagc aatttgctga ttatcaaacc cgttatgcag catatattgt
    84601 catagaaaga gatcagcaga ttgcagtaat cgaggctcca aatggtgctt tttttcttcc
    84661 tggcggggaa attgaaggag aagagacgaa agaagaagcg attgtccgtg agctgataga
    84721 agaaatggga attgtggcag aaatttcttc ttatctggga gaagctgatg agtattttta
    84781 ctcgaattat cgtgcaactt attattataa tccaggatat ttctttgttg ctgattcttg
    84841 gaagcgtata ggagaaccaa ctgaaaagac caataaaatt tggtgggtga gccctcaaga
    84901 agcgcttgtg aaattgaaac gtggtagcca ccgatgggca gtcgagagat ggatcgaaaa
    84961 aaataaaaac tgaaagaaag cgagtagatc agctttctct cagttttttt atttattaca
    85021 gtacatattt tttgattgct tcagctacac cgtgctcgtc gtttgatgaa gtgatgacat
    85081 ttgctgcttc tttgacaaga gaaacggcat ttcccattgc tactcctaga ccagcatatt
    85141 caatcatgga taagtcgttc tcgttatctc cgatagccat gatttcatct tgatcaatac
    85201 caaggtgctg tgcgagattc gcaactgccg cacctttact tgcttcttta tttagtactt
    85261 cgaagtaata gggcgcactt ttgaccgttg tgtatttatc acggaaagtt tgcggtaaac
    85321 gcgcaattgc tgcatctaaa atttctggtt cgtcgatcat catcattttg acgatgttca
    85381 tgtctggtgt catttcttca ggtgttcgat attttagcgg catcttgact aaacttgctt
    85441 cgtggatcgt ataagcgctg atatctcgat taggcgtgta gatcgctttg tcatcgatcg
    85501 aatggagatg actgccgaca cggcgtgcca ttacttcgat ctctaggaaa tcttcatgtg
    85561 tcagtccgtg acgggcgatg attttacctg atttcgtttg ttgtacgagt gccccgttat
    85621 aagtaataac gtaatcatta tcttgaaaaa ggttcaactc gtttaactgt tcctggacac
    85681 ctggtaaggg acggccagtg cataaaacga tcgaaacacc ttttgcaaca gcagcggcaa
    85741 tcgcttcttt tacttctttt gttacttctc ttttctcatt taataaagta ccatcgatgt
    85801 cgatagcgac tagtttgata gacataattt cctccaatat attttaattt atttcaatca
    85861 atgcgccatt acggatatgg ctggcaaatt cttgataagt ttcgtcaaat aaatcataat
    85921 gatcacgcaa ggacggatca accatttctt ttggaaagaa aaatcgctca tctccttgtt
    85981 cttgtcccgc taatgcagca acaagctgac taacctgtga taattctatc aaagttccgt
    86041 catttcgcaa aatctcgatt tgtgtgcgat gtctgccttg ttctgggcga taaaaatcat
    86101 aaggtagatc gtagctagaa ttcacagcag tgtaataaac aggattatat ccgacctttt
    86161 ctactaacaa agtgagttct tggactaatt ttgaatcccg gttgccagaa aaagtagcag
    86221 atttcagtgg tttacgattc aaaaaacgtt tagctaaatc atttaaaata ggatcagcag
    86281 attcagacca ttgagtaaag taagtgccca agaccccatc atcaagtttg aggtattctt
    86341 ctaaagtaaa gcttccgtca agaaatggca gaagtaattg agaatggagt tcaaaactgc
    86401 tggcatcttc ttgatacaac tcgtgtgcgc gatgaagcag atgctccaaa attacttcca
    86461 taccacggga aacaggatgg aagtacactt gcacatacat ttgatatcgg ctaacgatat
    86521 aatcttccac tgcatgcatg ccactcatgg aaaaagcgat cccgccttca taaggacgaa
    86581 tcacacgtaa tattcgggta agatcaaatg ttccatattc tgttccagtg aaataagcat
    86641 cccgaagcaa atagtccatt cgatctgcat cgatctgact agaaatcatt tggacaactt
    86701 gtgggttggg gtaagttttt tggatcacac tagctacctt ttcaggaaaa ccttcttcta
    86761 ctcggttaag aatctgatac acttctgttt ctggagaagt gatgatctct acagtgatgg
    86821 cttcatgatt cgtatggaag atatgttcaa atgtatgaga gtaaggacca tgaccgacat
    86881 catgcaacag tgccgcacat aaagtcacca gccgttcctg atcatcccag cctccgttgc
    86941 ctattttttc ttttgaaaaa tttcgagaaa agatatcgca gattcgtctt gaaatctcat
    87001 aaacgcctaa tgaatgagta aaacggctat gttctgctcc gtgaaaagtg aaagaagaag
    87061 tgcctaattg tttaatgcgt cgtagacgtt ggacttcttt cgaattgatc aaatctaaga
    87121 ttacttgatg ctggacatgg atatagttat ggactggatc acgaaaaact ttttccatag
    87181 gcagcatttg atatttataa ggggttgtca taatgtagct cctttatgtt attacgttgt
    87241 tccattctct ggatttggtt gttccattgc tccagctgga tttctttttt tagccagtcg
    87301 gtagaatcct gtaaatcact tgcagaatac agcttttcaa aagcttttgc aaatgcttct
    87361 ttgacttctt ttacaaaaag gggttggtca agcagttctt caagtgttgc cattgtagca
    87421 ggatccactg caggatagcc atttgtacca aagtcttctt ttaaagcaga ttgataaaat
    87481 cgcttcatga cttgcccacg tttttcctga ttaccgttga cacttaagta catcataaca
    87541 gaaatcccat ttttgatccg acgctgagcg ataccggcaa attttttgcc atggatgctt
    87601 aaatcataag ttccaggaca ataagaatgg gtgatttcga atgcttgtat cggtgccttt
    87661 ggaaaagcgt atttagtcaa taggagcatt tgctcgtaag cgtcatctat ggttattttt
    87721 tttgtgtcac tttgggggaa aatcaaagaa atattcaaaa ttccttcatc tgatacgatc
    87781 cctaatccac ctgaatttct gatcactgga gcgtaacctg catcttccag tgtttggatc
    87841 ccgtcactta gaaaaggagt tcgaacatcc ttcataccta agatgactgt gcagtcatat
    87901 tgccagaaat gtaaaaagat ttgctggtgt tctttagaat attccgttaa tatatctgtt
    87961 aatgcaaaag gggaaaagtc gtttttttga taaatgtgct gatctagtcg aatcgcaggg
    88021 ttcattcaac cggctccttt gtat
//
LOCUS       DAZBTF010000003        85032 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000003 DAZBTF010000000
VERSION     DAZBTF010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 85032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 85032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..85032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.003"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            101..619
                     /locus_tag="ACHGNS_000196"
     CDS             101..619
                     /locus_tag="ACHGNS_000196"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFN6936180.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            747..1430
                     /locus_tag="ACHGNS_000197"
     CDS             747..1430
                     /locus_tag="ACHGNS_000197"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFN6936181.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1739..1812
                     /locus_tag="ACHGNS_000198"
     tRNA            1739..1812
                     /locus_tag="ACHGNS_000198"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1773..1775,aa:Arg,seq:tct)
     gene            1971..2330
                     /locus_tag="ACHGNS_000199"
     CDS             1971..2330
                     /locus_tag="ACHGNS_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFN6936182.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2453..4084
                     /locus_tag="ACHGNS_000200"
     CDS             2453..4084
                     /locus_tag="ACHGNS_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFN6936183.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQFPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4146..4787)
                     /locus_tag="ACHGNS_000201"
     CDS             complement(4146..4787)
                     /locus_tag="ACHGNS_000201"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFN6936184.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4973..5049
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            5069..5377
                     /gene="rplU"
                     /locus_tag="ACHGNS_000202"
     CDS             5069..5377
                     /gene="rplU"
                     /locus_tag="ACHGNS_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFN6936185.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5404..5748
                     /locus_tag="ACHGNS_000203"
     CDS             5404..5748
                     /locus_tag="ACHGNS_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFN6936186.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5761..6054
                     /gene="rpmA"
                     /locus_tag="ACHGNS_000204"
     CDS             5761..6054
                     /gene="rpmA"
                     /locus_tag="ACHGNS_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFN6936187.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6488..9295
                     /locus_tag="ACHGNS_000205"
     CDS             6488..9295
                     /locus_tag="ACHGNS_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFN6936188.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9486..9977
                     /locus_tag="ACHGNS_000206"
     CDS             9486..9977
                     /locus_tag="ACHGNS_000206"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFN6936189.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            10080..11069
                     /locus_tag="ACHGNS_000207"
     CDS             10080..11069
                     /locus_tag="ACHGNS_000207"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFN6936190.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11102..11905
                     /locus_tag="ACHGNS_000208"
     CDS             11102..11905
                     /locus_tag="ACHGNS_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFN6936191.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11926..12840
                     /locus_tag="ACHGNS_000209"
     CDS             11926..12840
                     /locus_tag="ACHGNS_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN6936192.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12923..13297
                     /locus_tag="ACHGNS_000210"
     CDS             12923..13297
                     /locus_tag="ACHGNS_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFN6936193.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13436..13681
                     /locus_tag="ACHGNS_000211"
     CDS             13436..13681
                     /locus_tag="ACHGNS_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFN6936194.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13853..14917
                     /locus_tag="ACHGNS_000212"
     CDS             13853..14917
                     /locus_tag="ACHGNS_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFN6936195.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15163..15588
                     /locus_tag="ACHGNS_000213"
     CDS             15163..15588
                     /locus_tag="ACHGNS_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFN6936196.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15581..16030
                     /gene="nusB"
                     /locus_tag="ACHGNS_000214"
     CDS             15581..16030
                     /gene="nusB"
                     /locus_tag="ACHGNS_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFN6936197.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            16127..16981
                     /locus_tag="ACHGNS_000215"
     CDS             16127..16981
                     /locus_tag="ACHGNS_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFN6936198.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            16982..18319
                     /gene="xseA"
                     /locus_tag="ACHGNS_000216"
     CDS             16982..18319
                     /gene="xseA"
                     /locus_tag="ACHGNS_000216"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFN6936199.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18324..18548
                     /locus_tag="ACHGNS_000217"
     CDS             18324..18548
                     /locus_tag="ACHGNS_000217"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFN6936200.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18550..19440
                     /locus_tag="ACHGNS_000218"
     CDS             18550..19440
                     /locus_tag="ACHGNS_000218"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFN6936201.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19449..20267
                     /locus_tag="ACHGNS_000219"
     CDS             19449..20267
                     /locus_tag="ACHGNS_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFN6936202.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20374..20823
                     /locus_tag="ACHGNS_000220"
     CDS             20374..20823
                     /locus_tag="ACHGNS_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFN6936203.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20841..22523
                     /gene="recN"
                     /locus_tag="ACHGNS_000221"
     CDS             20841..22523
                     /gene="recN"
                     /locus_tag="ACHGNS_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFN6936204.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22578..22694)
                     /locus_tag="ACHGNS_000222"
     CDS             complement(22578..22694)
                     /locus_tag="ACHGNS_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFN6936205.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22767..23723)
                     /locus_tag="ACHGNS_000223"
     CDS             complement(22767..23723)
                     /locus_tag="ACHGNS_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFN6936206.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23894..24262
                     /locus_tag="ACHGNS_000224"
     CDS             23894..24262
                     /locus_tag="ACHGNS_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFN6936207.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24432..24863
                     /gene="mraZ"
                     /locus_tag="ACHGNS_000225"
     CDS             24432..24863
                     /gene="mraZ"
                     /locus_tag="ACHGNS_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFN6936208.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24884..25843
                     /gene="rsmH"
                     /locus_tag="ACHGNS_000226"
     CDS             24884..25843
                     /gene="rsmH"
                     /locus_tag="ACHGNS_000226"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFN6936209.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25848..26249
                     /gene="ftsL"
                     /locus_tag="ACHGNS_000227"
     CDS             25848..26249
                     /gene="ftsL"
                     /locus_tag="ACHGNS_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFN6936210.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26250..28442
                     /locus_tag="ACHGNS_000228"
     CDS             26250..28442
                     /locus_tag="ACHGNS_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFN6936211.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28468..29430
                     /gene="mraY"
                     /locus_tag="ACHGNS_000229"
     CDS             28468..29430
                     /gene="mraY"
                     /locus_tag="ACHGNS_000229"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFN6936212.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29444..30805
                     /gene="murD"
                     /locus_tag="ACHGNS_000230"
     CDS             29444..30805
                     /gene="murD"
                     /locus_tag="ACHGNS_000230"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFN6936213.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30831..31919
                     /gene="murG"
                     /locus_tag="ACHGNS_000231"
     CDS             30831..31919
                     /gene="murG"
                     /locus_tag="ACHGNS_000231"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFN6936214.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            31934..33154
                     /locus_tag="ACHGNS_000232"
     CDS             31934..33154
                     /locus_tag="ACHGNS_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFN6936215.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33310..34635
                     /gene="ftsA"
                     /locus_tag="ACHGNS_000233"
     CDS             33310..34635
                     /gene="ftsA"
                     /locus_tag="ACHGNS_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFN6936216.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34658..35899
                     /gene="ftsZ"
                     /locus_tag="ACHGNS_000234"
     CDS             34658..35899
                     /gene="ftsZ"
                     /locus_tag="ACHGNS_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFN6936217.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35916..36593
                     /locus_tag="ACHGNS_000235"
     CDS             35916..36593
                     /locus_tag="ACHGNS_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFN6936218.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36607..37206
                     /locus_tag="ACHGNS_000236"
     CDS             36607..37206
                     /locus_tag="ACHGNS_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFN6936219.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37287..37508
                     /locus_tag="ACHGNS_000237"
     CDS             37287..37508
                     /locus_tag="ACHGNS_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFN6936220.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37557..38339
                     /locus_tag="ACHGNS_000238"
     CDS             37557..38339
                     /locus_tag="ACHGNS_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFN6936221.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38360..39067
                     /locus_tag="ACHGNS_000239"
     CDS             38360..39067
                     /locus_tag="ACHGNS_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFN6936222.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39329..42127
                     /gene="ileS"
                     /locus_tag="ACHGNS_000240"
     CDS             39329..42127
                     /gene="ileS"
                     /locus_tag="ACHGNS_000240"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFN6936223.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42176..43702)
                     /gene="zwf"
                     /locus_tag="ACHGNS_000241"
     CDS             complement(42176..43702)
                     /gene="zwf"
                     /locus_tag="ACHGNS_000241"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFN6936224.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43717..44364)
                     /locus_tag="ACHGNS_000242"
     CDS             complement(43717..44364)
                     /locus_tag="ACHGNS_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFN6936225.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44548..44877)
                     /locus_tag="ACHGNS_000243"
     CDS             complement(44548..44877)
                     /locus_tag="ACHGNS_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFN6936226.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            45054..45782
                     /gene="sfsA"
                     /locus_tag="ACHGNS_000244"
     CDS             45054..45782
                     /gene="sfsA"
                     /locus_tag="ACHGNS_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFN6936227.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45798..46811
                     /locus_tag="ACHGNS_000245"
     CDS             45798..46811
                     /locus_tag="ACHGNS_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN6936228.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46811..48088
                     /locus_tag="ACHGNS_000246"
     CDS             46811..48088
                     /locus_tag="ACHGNS_000246"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFN6936229.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48151..50853
                     /locus_tag="ACHGNS_000247"
     CDS             48151..50853
                     /locus_tag="ACHGNS_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFN6936230.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            51005..51322
                     /locus_tag="ACHGNS_000248"
     CDS             51005..51322
                     /locus_tag="ACHGNS_000248"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN6936231.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51352..51672
                     /locus_tag="ACHGNS_000249"
     CDS             51352..51672
                     /locus_tag="ACHGNS_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFN6936232.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51780..53240
                     /locus_tag="ACHGNS_000250"
     CDS             51780..53240
                     /locus_tag="ACHGNS_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFN6936233.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53308..53529
                     /locus_tag="ACHGNS_000251"
     CDS             53308..53529
                     /locus_tag="ACHGNS_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936234.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53560..53742
                     /locus_tag="ACHGNS_000252"
     CDS             53560..53742
                     /locus_tag="ACHGNS_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFN6936235.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53742..54155
                     /locus_tag="ACHGNS_000253"
     CDS             53742..54155
                     /locus_tag="ACHGNS_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFN6936236.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54278..55459
                     /locus_tag="ACHGNS_000254"
     CDS             54278..55459
                     /locus_tag="ACHGNS_000254"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6936237.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(55530..55694)
                     /locus_tag="ACHGNS_000255"
     CDS             complement(55530..55694)
                     /locus_tag="ACHGNS_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936238.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            55848..55921
                     /locus_tag="ACHGNS_000256"
     tRNA            55848..55921
                     /locus_tag="ACHGNS_000256"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55882..55884,aa:Arg,seq:cct)
     gene            complement(55990..57129)
                     /locus_tag="ACHGNS_000257"
     CDS             complement(55990..57129)
                     /locus_tag="ACHGNS_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN6936239.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            57301..57435
                     /locus_tag="ACHGNS_000258"
     CDS             57301..57435
                     /locus_tag="ACHGNS_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936240.1"
                     /translation="MIKVIFKRITELPEFRLFVDYWYVWFFIVALLIGLDVILERKLK
                     "
     gene            complement(57428..58063)
                     /locus_tag="ACHGNS_000259"
     CDS             complement(57428..58063)
                     /locus_tag="ACHGNS_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936241.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(58176..58811)
                     /locus_tag="ACHGNS_000260"
     CDS             complement(58176..58811)
                     /locus_tag="ACHGNS_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFN6936242.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(58845..59306)
                     /locus_tag="ACHGNS_000261"
     CDS             complement(58845..59306)
                     /locus_tag="ACHGNS_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFN6936243.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(59436..59867)
                     /locus_tag="ACHGNS_000262"
     CDS             complement(59436..59867)
                     /locus_tag="ACHGNS_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFN6936244.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(59885..60205)
                     /locus_tag="ACHGNS_000263"
     CDS             complement(59885..60205)
                     /locus_tag="ACHGNS_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN6936245.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            60504..61280
                     /locus_tag="ACHGNS_000264"
     CDS             60504..61280
                     /locus_tag="ACHGNS_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFN6936246.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            61295..61498
                     /locus_tag="ACHGNS_000265"
     CDS             61295..61498
                     /locus_tag="ACHGNS_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6936247.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            61514..61852
                     /locus_tag="ACHGNS_000266"
     CDS             61514..61852
                     /locus_tag="ACHGNS_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFN6936248.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            61839..62018
                     /locus_tag="ACHGNS_000267"
     CDS             61839..62018
                     /locus_tag="ACHGNS_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936249.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(62061..62531)
                     /locus_tag="ACHGNS_000268"
     CDS             complement(62061..62531)
                     /locus_tag="ACHGNS_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936250.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            62618..63316
                     /locus_tag="ACHGNS_000269"
     CDS             62618..63316
                     /locus_tag="ACHGNS_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFN6936251.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            63494..63835
                     /locus_tag="ACHGNS_000270"
     CDS             63494..63835
                     /locus_tag="ACHGNS_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936252.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            63828..64499
                     /locus_tag="ACHGNS_000271"
     CDS             63828..64499
                     /locus_tag="ACHGNS_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFN6936253.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            64505..65197
                     /locus_tag="ACHGNS_000272"
     CDS             64505..65197
                     /locus_tag="ACHGNS_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFN6936254.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            65194..65943
                     /locus_tag="ACHGNS_000273"
     CDS             65194..65943
                     /locus_tag="ACHGNS_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6936255.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            65955..66224
                     /locus_tag="ACHGNS_000274"
     CDS             65955..66224
                     /locus_tag="ACHGNS_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936256.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            66230..66394
                     /locus_tag="ACHGNS_000275"
     CDS             66230..66394
                     /locus_tag="ACHGNS_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936257.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            66387..66686
                     /locus_tag="ACHGNS_000276"
     CDS             66387..66686
                     /locus_tag="ACHGNS_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936258.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            66686..66988
                     /locus_tag="ACHGNS_000277"
     CDS             66686..66988
                     /locus_tag="ACHGNS_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFN6936259.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            67028..67393
                     /locus_tag="ACHGNS_000278"
     CDS             67028..67393
                     /locus_tag="ACHGNS_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936260.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            67883..68023
                     /locus_tag="ACHGNS_000279"
     CDS             67883..68023
                     /locus_tag="ACHGNS_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936261.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            68020..68202
                     /locus_tag="ACHGNS_000280"
     CDS             68020..68202
                     /locus_tag="ACHGNS_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936262.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            68215..68409
                     /locus_tag="ACHGNS_000281"
     CDS             68215..68409
                     /locus_tag="ACHGNS_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936263.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(68442..68654)
                     /locus_tag="ACHGNS_000282"
     CDS             complement(68442..68654)
                     /locus_tag="ACHGNS_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936264.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            68782..68982
                     /locus_tag="ACHGNS_000283"
     CDS             68782..68982
                     /locus_tag="ACHGNS_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936265.1"
                     /translation="MKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDKY
                     LGEGFCGDSLFDDERILKEETE"
     gene            68979..69251
                     /locus_tag="ACHGNS_000284"
     CDS             68979..69251
                     /locus_tag="ACHGNS_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HFN6936266.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            69252..69437
                     /locus_tag="ACHGNS_000285"
     CDS             69252..69437
                     /locus_tag="ACHGNS_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936267.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            69415..69771
                     /locus_tag="ACHGNS_000286"
     CDS             69415..69771
                     /locus_tag="ACHGNS_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936268.1"
                     /translation="MKKVNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVL
                     KIEQNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIR
                     GGVCEISLVKRLPRTR"
     gene            69731..70207
                     /locus_tag="ACHGNS_000287"
     CDS             69731..70207
                     /locus_tag="ACHGNS_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFN6936269.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(70338..71066)
                     /locus_tag="ACHGNS_000288"
     CDS             complement(70338..71066)
                     /locus_tag="ACHGNS_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFN6936270.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            71114..71392
                     /locus_tag="ACHGNS_000289"
     CDS             71114..71392
                     /locus_tag="ACHGNS_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936271.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            71394..71780
                     /locus_tag="ACHGNS_000290"
     CDS             71394..71780
                     /locus_tag="ACHGNS_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFN6936272.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            71777..72157
                     /locus_tag="ACHGNS_000291"
     CDS             71777..72157
                     /locus_tag="ACHGNS_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFN6936273.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            72269..72721
                     /locus_tag="ACHGNS_000292"
     CDS             72269..72721
                     /locus_tag="ACHGNS_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFN6936274.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            72718..74445
                     /locus_tag="ACHGNS_000293"
     CDS             72718..74445
                     /locus_tag="ACHGNS_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFN6936275.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            74509..75696
                     /locus_tag="ACHGNS_000294"
     CDS             74509..75696
                     /locus_tag="ACHGNS_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFN6936276.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            75668..76237
                     /locus_tag="ACHGNS_000295"
     CDS             75668..76237
                     /locus_tag="ACHGNS_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFN6936277.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            76250..77614
                     /locus_tag="ACHGNS_000296"
     CDS             76250..77614
                     /locus_tag="ACHGNS_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFN6936278.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            77616..77894
                     /locus_tag="ACHGNS_000297"
     CDS             77616..77894
                     /locus_tag="ACHGNS_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFN6936279.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            77875..78201
                     /locus_tag="ACHGNS_000298"
     CDS             77875..78201
                     /locus_tag="ACHGNS_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFN6936280.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            78191..78529
                     /locus_tag="ACHGNS_000299"
     CDS             78191..78529
                     /locus_tag="ACHGNS_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936281.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            78519..78884
                     /locus_tag="ACHGNS_000300"
     CDS             78519..78884
                     /locus_tag="ACHGNS_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFN6936282.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            78891..79517
                     /locus_tag="ACHGNS_000301"
     CDS             78891..79517
                     /locus_tag="ACHGNS_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFN6936283.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            79517..79981
                     /locus_tag="ACHGNS_000302"
     CDS             79517..79981
                     /locus_tag="ACHGNS_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936284.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            80176..82485
                     /locus_tag="ACHGNS_000303"
     CDS             80176..82485
                     /locus_tag="ACHGNS_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFN6936285.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            82497..83201
                     /locus_tag="ACHGNS_000304"
     CDS             82497..83201
                     /locus_tag="ACHGNS_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFN6936286.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            83198..>85032
                     /locus_tag="ACHGNS_000305"
     CDS             83198..>85032
                     /locus_tag="ACHGNS_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFN6936287.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
BASE COUNT        28657 a        13995 c        17863 g        24517 t
ORIGIN      
        1 tataattatt aaagggaaac atttttacag ctataatttg aaaaaattta tcgttattat
       61 atgattaaga aggattgaat aaagaaagaa gttgaaaacg atacctgatt acaaaaaaat
      121 aacttttata agttctataa cgttgattat atttttaatt cttattcttc tcgtcgtctt
      181 ggttccccga atttttggtt tgactcctta tattattgag gacaaagaaa tgagtccata
      241 ttatgcacaa ggaaatctga tatatgtacg accgaaaaat acaaatgaga ttttagtcgg
      301 agatattatt acatattatg aaaattcagg aaaaaaaatc tcgacgcgtc gcgtaatagc
      361 cgttgaaaac aaccacgaag atttttatac aaaagctgat acaaagactt atattgagcc
      421 tggagtagta aaaaagagaa atattatcgg tagtcccatc tttcagattc catatattgg
      481 actagtcctt tcaaaaacgg catttgcatg gattcagggg ttattcttta cgattgcttt
      541 ttgcctaaca ggaatcacag catggaatac ctttgtagag ttgaaaaaga agcggagacg
      601 aacacagaaa tttttgtaag aatgttaaaa acataaatgt attccagaga tccaacgact
      661 cttctgaaca tgaaacacga gaaaacactc atgcagcttg tagatttagt cagttgttcg
      721 tataatgtgt gaagaggagg agtagtatgc gcaatgaaat gatgcatcgt cctgtagtca
      781 aagaagaact attggatttt atgcgtaccc agcaaaaaaa attaccgggt gagctaggca
      841 aactggaaga agaagctcat gtagcagaag taccagttat tccccatgaa acagttgtat
      901 ttttgaaatt tttacttgga cagcttcagc ctgaacgaat cttggagatc ggagcagcta
      961 tcggtttttc atcaagtgtc atggccacga caatcggaga aaacgctcat gttacgacaa
     1021 ttgatcgttt cgatgtcatg atcgagaaag ctaaaaaaaa ctatgaacgt ctaggactga
     1081 cagataaagt gactttgctt gaaggtcaag cagcagatat cttgccggag ttgtctggtc
     1141 cgtatgactt tatttttatg gatagtgcaa aatcaaaata tattgaattt ttacctgaat
     1201 gcttacgttt gttaagaaaa ggtggcgttt tgatggtaga tgacatcttc caaggaggaa
     1261 cgatcttgct cccagatgaa gagattcccc gtggcaaacg agctatccat cgtaaattaa
     1321 atgaatttct tcgtgttgtg atggatcatc ccgatttgac atcgacaatc ttgccattag
     1381 gtgatggtgt gatcttaatg acaaaggaat cagaaacaat cgagttgtga tacatgatta
     1441 tggcagcctg tagataatcg attgaatcga gggctcaatt cgaacttgat gtaaaagttt
     1501 ctatatttgt aagtcaatta aaggatacag gaaatctaaa taaacaggag tagcatgaag
     1561 tcaatgtagc tcctgttttc aattgataac cttagaacag ctgaaggaga atgtctcgtt
     1621 ttaaaagatt gatttagtag ttgggccaaa ataattcatt atttgctaat taaataacta
     1681 tttcgtattg acagaagcaa tttaattatc tataattatt gatgttccgc tgattcttgt
     1741 catagtagct cagctggata gagcatccgc cttctaagcg gacggtcggg ggttcgaatc
     1801 cctcctgtga cgctaaaaaa ccgttagaaa ctgaggtttc taacggtttt tctttgtttt
     1861 ttgtgccaat attgtttgtt ttcaaaggca aataaaataa aaaaacatcc ttatcgagta
     1921 caattataag gagcaaaaaa ataaaactca tcagaaaaga ggaagaagat atgacgatac
     1981 tatctagaat tttagtgact ctagttgcaa ttgaattttt ttatatcatg tatatcgaaa
     2041 cggtgagaac tgattctgac acgacaagta gggtttttaa aatgagtaaa gaagagttaa
     2101 gcagaaaatc tgttcaaaca ttgtttaaga accaaggagt gtacaatgga ctattaggag
     2161 tgggactact ttatggagct tatctttctt ccgcatctaa agaaatcaca agtatgctat
     2221 taatatctat tttttttgtt gcattatatg gcagtttgac tagcgatcgt tggattttac
     2281 tcaaacaagg tgggctagct ctgttggcat tgctttctat tttcttataa atatagaaaa
     2341 aataagaatt tatctaacag gaaactttgt gaaaaatatt actttatctc atgaaatatg
     2401 atattctaat gaatggtatt ttagaaaatt tcatgaaaaa ggagttagat aggtgttcaa
     2461 aaattatgtt tcctcactaa aaaaagaatt ttcaggttat gatctttcaa gatttcagaa
     2521 agatcttttg gctgggatca cagtagctgc tgtagcattg cctttagcct tggcttttgg
     2581 ggtatcaagc ggagctgatg cggccgcagg tttgattacg gctatcattg ccggtatcgt
     2641 tattggcggt ttatcaggcg gtttttatca aatatcaggt ccgacaggtg cgatggcagc
     2701 tatcttgatg tctattgcgg caacaaaggg aatgcaaggt gtattgcttg ctactttttt
     2761 agcgggtcta tttttacttg cagcaggact tttccgatta ggaacactga cttcttttat
     2821 cccagcaccc gtaattacgg ggtttacttc aggtattgct atcattattg cattaggtca
     2881 gatcgataat ttatttggtg ttcactcgga aggtacaaac gttattgaaa aactaagtag
     2941 ttatcaaacg ataggatttc cgataaacac agcctctttg atcatgggag ggctagtcat
     3001 tttagggatg tttatctatc caaaaaaatg ggcgaaacga atgcctagtt cgctgctggc
     3061 tatcattttg gcaacagcag ttatgctatt gttccattta ccagtagcaa ctgtgggtaa
     3121 gattccacaa acattgatca gtagtaatcg tttgaatatg ggagatttta gcttttctgc
     3181 cttgcaacaa gtggcagttc cagctattag cattgcttta ttaggaatga ttgaaagctt
     3241 gctatgtggt gcttcggctg gccgaatggc aaataagccc ttagatagca atcaagaatt
     3301 aatggcgcaa ggaattggaa atctgttgct tcctttcttt ggcgggattc ctgctactgc
     3361 ggccatcgcc cgtacaagcg tggccatcaa atctggtgca caaactagat tagctggtat
     3421 gatacatgca gttgtcttac tgctctccat gcttgttttt gcgccagtga tgtcacagtt
     3481 tccaatgcca gcacttgcag gtgttttgat tgtgacggct tggcgtatga atgaatggga
     3541 aacgatcaag gagctattta ctaataaata ttggtcagca gttctattgt ttatccttac
     3601 gatgatttgt acagttatct ttgatttaag tattgctatc gtgatcgggg tgatcggcgg
     3661 ctgtgtattt tttgttgtca aaagcgctgc tatcacgatt tcattagaaa cgatcgattg
     3721 gcaaagaatg gggctgccag aaacggaaaa gctgaataac tggacagtag tgtatattag
     3781 tggtccactg ttttttatgt cttcagaaaa actcaaaaat aaattggaag aactaaatca
     3841 tcacgatggt gtcattttct ccatgcgagg cgtaccaagt attgatttga cggcacgtgc
     3901 agtttttgat gaatttcaag aaaaagcaga gcaaagagga caaacgatta tctatacttc
     3961 cttgcagcca gaagtagaaa aacaactttc tcacttgtgg gaacatacat tcacgcatca
     4021 acatcaaaca gttgctcatg cgttgacagc tttacatgcg aaatatactg caattagcca
     4081 ttaaaacaat gaaaaagaac agacaagact gtaaactgaa gattttatgg tcttgtctgt
     4141 tctttttaat attgctgtaa aagtgaagct agtacttgcc agtcttgatg gataacaggt
     4201 acaagggagc gattgtagaa aatagctgct ggatgaaaaa gtggaagaat tcgatagttt
     4261 ttttctgacc attcgtaggc atccttttgt tctgttaatt gtaagacagg ccctgtatag
     4321 agttgaccat gatttttggt aaccgtatat tctttgccaa gtaaacgttg aagaccaatg
     4381 tttccaagtg tcgcaattaa ttttggctgg accttcatca gttcgtaatc caatattggt
     4441 gcatgcgcaa atacttcgga acgagtaggt gtccgatttg gataaacagt ttctgactgt
     4501 tgtgtccgtt tattgatccg atgagtcacg cgatacgggc ggctccttac agcactggtg
     4561 atatacacct cttctcgtgt taagccagtt gaagaaagtg cctgcatcaa ttcttttcca
     4621 gcttgaccgc taaaaggaac atgcgtatca atctctgttt tccctggggc ttctcccacg
     4681 agcatcagtt taggatgaag aggtccttgc ccagcgataa atccttctac aggacggccg
     4741 ttcattctta aagaagcttc ttgtatcagt tcttttgggt attccattta tgctcctcct
     4801 ttttctttta ttataagaat aaaagaaaca caatgcgact attttatttg gtagaactgt
     4861 gaggatagaa aagcagaagt attgcttgaa tttttgtttt actttttgat tttaaattta
     4921 gttgactaac ggttgctgtt ctggtaagct agtactgttg taattgtgga ctccacagct
     4981 acaaccgctc agaacaagta ataagcacag catatgctgt cacttcggat ggcgagtcta
     5041 agtatatcag aaggaggtgc tttatagcat gtacgcaatt attaaaactg gtggtaaaca
     5101 agtaaaagtt gaagtgggac aagcaatcta tgttgaaaaa cttgacgttg aagcaggaga
     5161 aaaagttgtt tttgacgaag ttatcttagt aggcggagaa tctacaaaag taggcgctcc
     5221 aactgttgca ggtgcaactg ttgaaggtac tgttgaaaaa cacggcaaac aaaagaaagt
     5281 cgttactttc aaatacaaac ctaaaaaaca ttcacaccgt aaacaaggtc atcgtcaacc
     5341 atatacaaaa gtggttatcg acgcgatcaa cgcttaatag cgtttgagaa agaaggccta
     5401 tatatgatca aaggaacatt taagcgaaat gacgcaggtc agatcgtttc attcactttg
     5461 accgggcatg ctgatgctgg tccttatgga agtgatatcg tttgtgcggg tgtttctgcg
     5521 ttagcgatca gcactgtcaa cgggattgcc tcattagccg gttttgagcc gattgttgaa
     5581 atgaatgaag aagaaggcgg ttatctttat accgaagtca cttcaggaat gacacaagaa
     5641 caaaacaata ttgctgagat tctattagaa aatcttttat taggattaca atcgatagaa
     5701 gctgaaaact cagaatatat ccaaattaaa acaattaatg ataaatagga ggtgcagacc
     5761 atgttgttat ctatgaatct acaattattt gcccacaaaa aaggtggcgg ttctacatct
     5821 aacggccgtg actcagaatc aaaacgtcta ggtgctaaac gtgctgatgg acaaacagtt
     5881 acaggtggat caattcttta ccgtcaacgc ggtacaaaaa tctatccagg tgtaaacgtg
     5941 ggtatcggtg gagacgacac tttatttgca aaagttgatg gcgtagtacg tttcgaacgt
     6001 aaaggccgta acaaaaaaca agtgtctgtt tatccagtag ctcaagaagc ttaattgaat
     6061 cttatgatta aagaggttgt gacaagactt ttgtcacagc cttttttgtt tataaaaata
     6121 aaagttgtag tggaggctac gaagtttact ttgtcttttt acacttaata tttttttgcc
     6181 aatttggaat caggatgttt tgataactat cgttttcaat tgatggaagc tatattgttt
     6241 gctatcagtg aaataattat atggtaaaca gtgttctttt tcttgtttcc attataaaaa
     6301 tcgtatcggg atttttcgtt cttttaaatt gaacatttct atctatccgc gtgttatcaa
     6361 caaaacaaaa tcaggtaaat agttgacgaa acagaaataa atacatagta ttatgttttg
     6421 aagcctaagt acaatagtct atagaaaagg agcgatacca ttggcaaaac gcattgaccg
     6481 aatttatatg tatgtcaaag aaaaaaccgc tcatttaaca aaggcagaat atgatcaagg
     6541 agtaacaaca caggaaattg ctgaagtgtt agggattcag cgaacaaata gcagtaaaga
     6601 tctgaatcag ctagtgcgcg aaggaaaact gataaagaca gacggacgac cagtacgtta
     6661 catatatcaa acactcgtta cgcacgtaaa gccagttaca aaacatgtag tcagttacaa
     6721 agaaacgccg gtctctgaag aaaaaaatac actccaaata gatactaagg atatctttgc
     6781 aaaaatcatt ggagctaacg gcagcatgaa aaattcagtg gaacaggcaa aagctgcgat
     6841 tttgtatccg ccaagaggat taaattgttt gattacagga cctaccggat caggaaagac
     6901 ttattttgca cacgcgatgt atcattttgc aaaagcgcat catgtaatag atgaggaaac
     6961 ggaactgatt gttttcaatt gtgctgatta tgccaataac cctgagcttt tgatgagtca
     7021 tttatttggg tacgcaaaag gtgcttttac aggcgcagat gaagaaaaaa tggggattat
     7081 cgatcaagcg gatggcggta tgctgttttt ggatgagatt caccggttgc cgcctgaagg
     7141 tcaagaaatg atcttttact ttatggatca tggaacttac agtcgtcttg gggaaacaac
     7201 gaaatcccat gaagcaaatg tacggatcgt aggtgcgacc acagaagacc caggttcttc
     7261 tctattagaa acttttgttc ggcgtatccc aatcaatatc aaacttccgg cttttgaaaa
     7321 acgtcctgca aatgagaaag tcgatttagt aaaaatcatg attgcccacg aagcaaaccg
     7381 cacgcagcga aagatttctt tgacagaaga tgtagcaaaa gcattgatcg gcagtgtgac
     7441 ctatggaaat ataggacaat tgaagtcaaa tatccagtta gtatgtgcac gaggatttct
     7501 aaatcatatg caatcacccg aaatctcgat cacaatcgat gatttgacag aagggattcg
     7561 tagcggattg attcaattag caagcaaccg cagtgcaatg gcagagcttt ctaaaatatt
     7621 ggagccaaag ataactgtag caccaaatga tacgatcatg aaaatccaat cggactccta
     7681 tgagttacct tataatctat atgatatcat cggagataaa gcagctttac tgaagtcgga
     7741 tggtttggat caagaagcaa tcaatcattt catttctaca gatatcaaca tccatctgaa
     7801 gtctttctat aaagaccatg gtttttcttt taatgcagac agcaaattag cagaatttgt
     7861 agatccgaaa atcatagaag tgaccaatca gatttatgta atggtcaaaa gatctttacc
     7921 atatgagttt cagcaaaact ttatttatgc tatgagcttg catatcagtt cttttttgaa
     7981 aaaaatcaac ttaggagaag aacggcatac gaatgataac atccgtgaga tggcttttga
     8041 ttttccagat gaatatgctg ttgcgaaaga agtgcgtagt tatatcgaaa cgtattttca
     8101 agtggagatt ccagaaagtg aagactatta cttgaccgtt ctactcgtct cgttacgggc
     8161 aaatcaagca agcggcagaa tcggtgtagt cgttgcagct cacggaaaca gcacagcaag
     8221 cagtatggtc caagttgtcc aacagttgtt agatgcagag aacgtacgag cagtagacat
     8281 gcctttggat atggatccga aaacagcatt ggaccgaatt gaacgcaatg tacaagaagt
     8341 agatgaagga agcggggtca tactcttagt agatatgggg tcccttgcgt cgtttaattc
     8401 gcaaattcaa agagacactg gaattccagt acgtactgtt gatatggtaa caacttctct
     8461 agttttggaa actgtacgaa aggtctcagt attagggaca gatctagata tgttgcatga
     8521 atcattgaaa aattttcgag gttacgcaga aatatcttct gtagaatctg ttcaggaagc
     8581 aaaacctatt cataagaaaa aagctgtttt ggctatctgc gcatctggcg aaggtacagc
     8641 acaaagaatc aaagaattga tcgaacgagc ggtaaacaag cgtcaagaaa cagaattaga
     8701 agttctagct ttgtcgattg tggaagtgaa agaagaactg ccagttatcc aaaaagaata
     8761 tcagatcata gctactactg ggattactga tttgaatatc caagccccat ttattccttt
     8821 agaacgtttt attgatcaaa atatcgaagt cattttggat cagttgctga tggaatcaga
     8881 attggaggaa actgaattca tctccctaga tgaagagtca gctaaaaata cgtgtgttga
     8941 gtttatctct gataacttca tctttattaa tggtagtaag ttaatcgatc caatgtggca
     9001 attttctaca caaatctctc agacgacggg aatcggcgat gaagaatatg gattcaaaat
     9061 caacctagtg atgcacacag caggaatgat cgaacgtatt attcggaatg aaccattgac
     9121 tgtggaagaa aacgaattga caaatacaac gaatgatcct ctttatagcc aattggcagc
     9181 ctcagttgtt ctgttggaag atcagataaa agtaaaagtg ccgatagaag aaatgtatta
     9241 tctgctacgt ctagtccata atcagttaga taaaaaagaa tacactgtgc catgaataca
     9301 caaaaacgat acacttttat agtgtatcgt ttttgtgtat tggttaagaa tcctttaaat
     9361 tcaattagtg tatcgtaaaa aagttggcac gctttttgct tatagtagtg tgtgctagga
     9421 ggtggttgtt cacacagaaa tgataggggt ttctgtgaag tagtgaggaa gaaggagttt
     9481 tgcagatgga tattcgattg gcacgtatag atgatcgttt aatccacggg caagtcgcaa
     9541 cagtttggtc gaagcaaaca ggtgttgaac gtatcattgt gatcagcgat gtagtcgctc
     9601 aagataaatt gagaaagttt ctactaaaag aagctgcacc gccggggatc acagcaaatg
     9661 taataaccgt gcagaaaatg attcaaatct atcatagtgg attattatca cgagcaaaag
     9721 tgatgctttt attcaccaat ccccaagatg ttgaagcggt tgtccgctgc ggtgtacagt
     9781 tgaagtcgtt gaatatcggc ggtatgagtt ttactgaagg caaatcaatg attaccaatt
     9841 ttgtttcagt aaatcaacaa gatattgatt cctttcgtta cttgaacagt caagaagtgg
     9901 agttagagat tcgaaaagta cctgctgaca ggaaaatcct tttgatggaa ctactagcga
     9961 aaatcaaaaa ggtgtagcaa gaatggttgt tggaaaaggt caaataattc tgttgtaatg
    10021 gttagcaatt attaatattt ttgaatttgt tacaacaaaa gtaaatagga ggtataaata
    10081 tggtagggat tatcctagca agtcatggac aatttgctga aggaatctta caatccggtt
    10141 caatgatctt tggcgaacaa gaaaacgtca aagccgtgat tttgaaacca agtgaaggtc
    10201 cagatgactt gagagccaaa ttggaagaag cggttgcttc ttttgataat caagacgaag
    10261 tgttattctt agttgattta tggggcggaa caccgttcaa tcaatcaaac acattattcg
    10321 aagagcacaa agataaatgg gcaatcgtca gcggtttgaa tttaccaatg ttgattgaag
    10381 cctacgcatc acgcttttca atggaatctg ctcatgaaat tgcagcacac atcattgaaa
    10441 cagcaaaaga cggtgtaaaa gtaaaaccgg aagaattaga accggcagaa gcaccgaaag
    10501 cagcagttga agacgcacaa cctaaaggag cacttccaga aggaactgtg gtcggagacg
    10561 gaaaaatcaa atatgtatta gcacgtgttg actcacgtct gttacatgga caagttgcga
    10621 cagcttggac aaaagcagtg caaccaaacc gtattatcgt tgtttcagat gcagtatcaa
    10681 aggatgatct gcgtaagaga ctaatcgaac aagctgctcc tccaggtgta aaagcaaatg
    10741 ttattcctat tagcaaaatg atcgaagtag caaaagaccc tcgttttgga aataccaaag
    10801 cattgctttt atttgaaaat cctgaagacg tattgacagc tgttgaaggt ggcgtagaca
    10861 tcaaagaatt aaatgttggg tcaatggcgc attcggtcgg taaagtcgtt gtaagtaaag
    10921 tattgtctat gggacaagaa gacgttgaag cgtttgagaa gcttgaacaa aaaggtgtga
    10981 aattcgatgt acgtaaagta cctaatgatt cacgcgataa catggatgat attttgaaaa
    11041 aagcaaaagc tgaattagca aaagcgtaat aaataatatt aaaatttata ggaggcttat
    11101 catgtctatt atttcaataa ttttagtcgt attagttgcc tttctagctg gtatggaagg
    11161 tattcttgat gaatttcaat ttcatcaacc actagtggca tgtacattga tcggtttagt
    11221 taccggtaac ctcgaagctg gtatcgtact tggcggtacg cttcagatga tcgctttagg
    11281 ttgggcaaat atcggtgccg ctgtagcacc agatgctgca ttagcatcag tagcatcagc
    11341 aattatttta gttttaggtg gacaaggtgt taaaggtgtt ccttctgcga tcgcgattgc
    11401 cgttcctttg gctgttgcag ggttgttctt aacaatgatc gtacgtacaa tcgctgttcc
    11461 aatcgttcat ttgatggatg cggctgctga agaaggaaac atccgtaagg ttgaaatgtg
    11521 gcacatcatt gctgtatgtt tacaaggggt tcgtatcgcg gtcccagcag cagcgttgct
    11581 atttatccca gcacaaactg tgcaatcatt ccttgaatca atgccagcat ggttaacaga
    11641 tggtatggct atcggtgggg gaatggtcgt agctgtaggt tacgctttgg taatcaacat
    11701 gatggctaca aaagaagtat ggccattctt cgttattggt ttcgttgtag ctgcaatttc
    11761 tcaattaaca ttgattgctc taggtgcttt aggtgttgca ttagctctta tttacttgaa
    11821 cctttctaaa atgggtggtt catcaaataa tggaggcgga agcaactctg gtgacccttt
    11881 aggcgacatt ttgaatgact attaatcttg aaggaggaaa ataaaatggc agaagaaaaa
    11941 atcaaattat caaaaaaaga tcgtttagct gttgcgtggc gctctacctt cattcaaggt
    12001 tcatggaact acgaacgtat gcaaaacggt ggctgggcat tttctatgat tccagcaatc
    12061 aaaaaattat acaaaacaaa agaagaacgt tctgcagcat taaaacgtca cttggaattc
    12121 tttaatacac acccatacat tgcttctcca attttagggg ttacattggc tctggaagaa
    12181 gaacgtgcga atggcgcgcc tgttgatgat gtagcgattc aaggggttaa agtaggtatg
    12241 atgggtcctt tagctggtgt tggtgaccca gtgttctggt ttacagtacg tccaatgcta
    12301 ggtgcactag gtgcttcatt agctatggga ggaaatatcc ttggaccaat catcttcttc
    12361 ttaggttgga acattattcg ttgggcattc atgtggtata cacaagaatt tggctacaaa
    12421 gctggttcta aaattactga tgacctttct ggcggattgt tacaagacgt aacaaaaggt
    12481 gcttcaattt tagggatgtt cgtattagct gcccttgtac aaagatgggt atcaatcaaa
    12541 ttcttaccag ttgtatcaac tgtaaaatta gataaaggtg cttacatcga atgggataaa
    12601 ttgccttcag gtggtgaagg aatcaagagt gcatttgaac aagttaataa tggcttagcc
    12661 ctttctcctg aaaaggtaac aacattacaa gacaacttag accaattgat tcctggttta
    12721 gctgcattag gattaacatt gttatgtatg tggttgctta agaaaaaggt ttctccaatc
    12781 gttatcatcc ttggtttatt cgtagtcggt gttgttggac acgtcatcgg acttttataa
    12841 tccataaaaa tatagctcgg gcgttgtccc gagctttttt tatatagtat actgttcata
    12901 aacaaatatc tggagggaga aaatggttca atcaatcaat acaaaagtag acttggtaat
    12961 tgatgctact tcgtttacgg gtctgacaga ttacgggcaa atcatgatag gtaataatgg
    13021 ttttgagttt taccatgcgc gagatagacg caaatatatc caaattccat gggaagaagt
    13081 ggattatgta ctagcatcag tcatgctaaa gggaaaatgg attcctcgat atgccattca
    13141 aacgaaaaaa aatggtacat acacattttc ttccaaagaa cctaaaaaag tgcttcgtgc
    13201 gatcagagaa tatgtagatg aagatagaat ggtacaatca ttgagtttct ttgatgtagt
    13261 gaaacgtggt attcgtagta tgtttaaacg caagtaaaaa ataaagaaaa cttctaagag
    13321 gaggttttct ttattttttt actttgcaga agaaaatatg aaatagcaaa accaattatt
    13381 tgtccccttt aaatcccata tgctaaaatt gattagcaaa gaaggaggtt atattatgag
    13441 ttggttatgg acattgattg ttggagccgt gatcggagca attgcaggtg cgatcaccag
    13501 taaaggaaat tcgatgggct gtgtaacgaa tatcattgct ggactagtcg gttcaagtat
    13561 tggacaagcg attttcggcg aatggggacc aaaagctgca ggtatggcac ttattccatc
    13621 tattttaggc gcagtgattt tggtagcagt tgtctcattt ttctttggaa aacgatcata
    13681 agtttttaca aaatagtaaa ctaaaaggag ttgcagcgag actgtgactc ctttttggct
    13741 ttatttattt aaaataaaaa gaagttttcc ggaaattttt atttaactgt cgtgtacgaa
    13801 gaaaagtttg ctataatgga aaaaagcgtg caaagaaaga gggagtttca ttatgatgct
    13861 acgtgtagaa aaattgcgca aaaaaatgca agaagaaaat ttggattcat ttttggttac
    13921 cagtccgtac aatcttcgtt acttaacgaa ctttacaggt acgacaggat tagctgttat
    13981 tactttggaa aaagcatttt ttattactga ttttcgttat accgagcagg cggcagcgca
    14041 ggctcaaggg tttgaaatta ttaaaaatgt cggacctatt tttgatgagg tagcaaactt
    14101 agtacgcaaa gaagaactgc actcattagc ttttgaggaa acaacagtct catttttaga
    14161 atattcggtt ttagaagaaa tcatcgatgc ggaattagta cctgtttcac agatgatcga
    14221 agaactcaga gaagtgaaag atgaagaaga aatcgctatt atcgaaaaag catgtcatat
    14281 tgcggatatg gcatatgatc acatcctgaa aatgatccaa ccgggcatga ctgagattga
    14341 agtcgcaaat caactagact tttatatgcg ttctcttggc gcttcaggtg tttcttttga
    14401 tacgatcgtt gctagcggat tgcgttcggc tatgccgcat ggtgtagcaa gcaaaaaaat
    14461 catcgaacaa ggtgacttga tcacattgga ctttggttgc tattacgaag ggtacgtttc
    14521 cgatatgaca cgtacattcg ctattggtga tcctggggaa aaattaaaag aaatttatca
    14581 aatagtctta gatgctcaat tagcagttat tgatgctgca aaaccaggaa tgactggtgt
    14641 ccaattagat gcagttgcta gagattatat ttcaaaacat ggttacgggg aagcatttgg
    14701 acattctaca ggacatggga tcggtttaga gatccacgaa ggaccaaacg tttcttctcg
    14761 tgcagaaaag caatttgtac caggaaatgt gatcactgat gaaccaggga tctacttacc
    14821 aggaatcggt ggagtacgta ttgaggatga cttgttgatc acagaagatg gaaatcgtgt
    14881 gttgactcgt tcaccaaaag aattgatcat cttatagttt catcaataga agaagtaagc
    14941 gtaaaaatca gtgttcacag actttgttat agaaaatcac tcgatttaat gatacactat
    15001 agaagaactt ttaagctgta agagataata aagttgggtg ttttctccaa ttcatagcag
    15061 tttgcagctg atcggcggca gttttcttac cacgtgatca ctatgccgca tcatagagaa
    15121 ttcggatgta tctcgtgctt aggcaaaggg aggaataaat aaatggcaga tgaaaaaaat
    15181 ttgattttaa acgcaaacca agatttagga gagatcgtga tcgctcctga agtcatcgaa
    15241 gtaattatcg ggattgcagc ttctaaagta gaaggcgtat acggaatgcg cggaactttt
    15301 gccagcaatg tcactgaatt attaggacgt gcagctcacg gaaaaggtgt ttacttagca
    15361 aacgaggaag atggcttgaa agtagatctt tactgttatc tagaatatgg tgtttcagtt
    15421 cctaaagtag caatggaaat gcaggaacgg gtcagccagc aagtcctttt catgacggat
    15481 atcgatttat ctgaagtcaa tatccatgta gtagctgttg ttcctgaaaa attagaacaa
    15541 ccaacaatcg aagaactttt tgaagacgaa gaggaagaga atgaataaag aattatcaag
    15601 acacgagatt cgggaaatgg cattgcaggc tttatttcca ttagacttca atgcagacct
    15661 aacaaaagaa gatgcgattt tcaacgcaat cgaattagac caccgcgaca tgatcaacga
    15721 agatgaatca gagtttgttc ctgtttatct tgatacgcta gttggcggtg tctgtgcaaa
    15781 aaaagatgaa ttggataaag tgattgaaaa acatttaaaa aacaattggc acatcaatcg
    15841 gatctcaaaa atggatttgg tgatcttgcg tatcgcgatt tttgaaatga cttatgtgac
    15901 agatgtacca gcggctgttg ctttaaatga agcaatcgag ttggcaaaaa catttagtga
    15961 tgaccgttca cgtaaattcg tcaacggtgt tttatccaat gtattgaaag aattagaagc
    16021 tggggcgtaa gtcctagctt ttttctatga atcatcataa acctgttata aatcgggaaa
    16081 attctatggt agaatgaaaa cacaaaaaat atctgggagt ggttagatgg cagaattgat
    16141 taacggaaaa gaattagctg aaaaaatgca ggcagagatt gctgaaaaaa tccaaaaatt
    16201 gaaagataat ggtatccatc ccggattagt cgttttattg gtaggggaaa atccagccag
    16261 ccaaatctat gtaagaaata aagaacgtgt agcaaaagaa atcggaatcc attcattggt
    16321 agagcgttat cctgagacga tttctgaaga cgaactactt gcagaaattg aaaaatacaa
    16381 tcaagatcct agttttcatg gtatattggt ccaattacca ttgccaaagc atatagatga
    16441 agaaaagatc ctgttagcta tccgccctga aaaagacgta gacggttttc atccgatgaa
    16501 tttaggcaga ctccttgcgg gtaaaccgga caagattcct tgtactccat acggcatcat
    16561 gaaaatgttt gaagcttatt ctatcgatcc ggcgggtaaa cgagcagtag ttatcggacg
    16621 aagcaatatc gtcggtaaac cgatggctca gctactcttg atggcagatg caactgttac
    16681 tgttgctcat tctaaaaccg aaaatctggc tgaactggca caagaagcag atattttagt
    16741 cgtagcaata ggacgtggac attttgtcac aaaagaattc gtaaaaccag gtgctgtagt
    16801 gatagatgta gggatgaatc gtgatgctaa cggtaagctg atcggtgatg tgaaatttga
    16861 tgaagtggag cctattgcag gctggatcac tcctgtgcca aagggtgtag ggccaatgac
    16921 tatcacgatg ctgatgtatc aaaccgtaaa atctgccgaa gcttttggag caggtgaata
    16981 gatggcacaa gaatatttaa ctgtcactac attaacgaag tatttgaaaa gaaaatttga
    17041 tgccgatcct tatttagaac gcgtatattt aacaggcgaa atatcgaatt tccgtttacg
    17101 tgccaatgcc catcaatatt tcagcttaaa agatgaccat gcaaaaatat ctgctattat
    17161 gttcaaagga gcattccaaa aattacgatt ccagccaaaa gaaggaatga aagtccttgt
    17221 cgttgggcgt atttcattat atgaagcggg aggttcttat caaatctatg tagagcacat
    17281 ggagcctgat ggaataggtg ctttatatca agaattggaa gaacgaaaag aaaagctgat
    17341 gaaggaaggc ctttttcaag ggcctaaaaa aatgctgcca cgtttcccaa agcgaatcgc
    17401 ggtccttacc agtccaagcg gagcggttat ccgcgatatc atcacgacgg tgaaaagaag
    17461 atatccaatt gtccaattgg ttttgttccc aacagttgta caaggaaacc aagcagcgga
    17521 caatgttgtc aggaatatcc agagagtaga agagatgggc aatttcgata caatgatcat
    17581 cggtcgtggg ggtggttcga ttgaggattt atggccgttt aatgaagagc gggttgctcg
    17641 ggcgatttat caagctaata cgccggtgat ttcatctgta ggacatgaaa cagataccac
    17701 gatcgctgat ttagtagcag atgttcgggc tgccactcca acagcagctg cagaattagc
    17761 tgttcctgtt ttgtcagaag agttgatgcg cattcaagaa cggcaagcta gattgcagca
    17821 ggcgtttgta cgccagatcc agcgcaaaca agaacggttc gagcgtgctc gacgttccta
    17881 tgtatttcgg cagcctgaaa gattgtatga aggtcagacc atcaaattag accagttgaa
    17941 acagcgatta tatcaagcta cacagcaaat ttatcatgcg aaagaaaaac aaacgatcgc
    18001 attaagccac caattggaac aagtggctcc gctttatcga gttaaagcag ctcgccaaga
    18061 aactgcttat ctagaaaaac gcttagaaga gaaaatccaa caatatatga aacaacagca
    18121 gcagcgtttt gctcaagctg ttcaatctct tgatttgttg agtccattga aaatcatggg
    18181 aagaggctac agctatacga cgttaaatga tcaagtagta aaaagtgtca ccgagattca
    18241 tcaaggggat ttgctgacga tccattatca agatggaatc gttaaaggac aagtacaaga
    18301 gatagacaaa ggagaataaa aatatggaaa atccaacttt tgaagagtca ctgcaagaat
    18361 tagaaaaaat tgtcatgcag ttagaacaag gagacgttcc attagaatca gctctagatt
    18421 cgtttaaacg aggaatggaa ttaagcaaac attgccaaga tacattagct aaagcagaag
    18481 agaccttgac gaaaatgatg acagaatcaa atgaagaagt tgattttgac ggaaacgagg
    18541 aaagctgaga tgagattaac tgacttttca gctattcatc tacctgctgt agaaaaagaa
    18601 atcttgtctt ttttagatga gcatacaacg gatagaggac tctatgatgc gatgagttat
    18661 tcagtgaaag caggcgggaa aagaatacgt cccttattac ttttaacggc cgttgcttcc
    18721 tttgatgagc ctattgatgt tcccgtctat caagtagcag ctgctttaga aatggtgcat
    18781 acgtattcct tgatccacga tgatttgcct gcgatggaca atgatgatct tcgtagagga
    18841 aaacctacca atcataaagt attcggcgaa gcattagcta tattggccgg agatggtttg
    18901 cttacaggag cattccagct aatcagtatg gcacacttag gtaattctcc taagcttctg
    18961 ctgcttcagc aattagctgt ttgtgcggga tcacaaggta tggttgctgg gcaagctgca
    19021 gatatcgagg gcgaatcgaa aaagctttct ttagaagaat tagcatttat tcatgaaaga
    19081 aagacagggc atttgattcg ttatgccttg ctggctggtg gcatattagc gaagcaacct
    19141 gaagaaatct tattgctact tcaacgatta gccgaacatt taggtttagc ttttcagata
    19201 agagatgatc tactcgatgt gattggtaca acgaaaacat tagggaaaac agctggtaaa
    19261 gatgaacgta tggaaaaaaa tacttacccg cgtttattag gcctagaaaa gacacgtgag
    19321 gcgttggaga ttgagctgtt atccgccaac aagatcatag acaaattaga agaggacgtt
    19381 ttttcttttg atggcgggct gatgcgccag atgatcaagc aatttgatgt ggagaaatga
    19441 gggaaagaat ggaaaaagaa cgcgtagatg tattagcggt caaacaagga ttatttgaaa
    19501 caagagaaca agccaaacgt tcagtgatgg ctggtttgat ttacaacgaa aaaaacgagc
    19561 ggtttgataa accgggagaa aaaattccag tgagtagtga attaagagtc aaaggtaaaa
    19621 aacttcctta tgtttctcga gggggattaa aattagaaaa agcactcaaa gcatttgatc
    19681 tatctgttga aggaaaaaca ttgttagata ttggtgcatc cactggcgga tttactgatg
    19741 cagctttgca aaatggcgca aaaatgagtt atgctttaga cgttggctac aatcagctag
    19801 cttggaaaat aagacaagat cctagagtcg tcgtaatgga aagagtgaat tttcgttatg
    19861 caaaacccga agactttacc ttaggaattc cagaagttgc tgttatcgat gtttctttta
    19921 tttcactaaa actgatgctg cctccattac atgctatttt aaaagaagag ggagaagtga
    19981 ttgctttaat caaaccccaa ttcgaagcag gcagagaagc tgtagggaaa aacggaattg
    20041 ttcgtgaccc tcagacccat caaaaagttt tagaagacat tttgtcattt gcggcacaag
    20101 gtggatatga tgtgctggaa ttaagttatt ctccgatcac ggggggagaa ggaaatatcg
    20161 agttcttggc tcacttgagg aaagtacccg aaagcggaac aatcaattca gctattaata
    20221 tggcagaagt ggtttcaaat gcacacgaac agtttgacca taaataaatt gtaactgtga
    20281 aaatcaagtg agaagtatgc ggattcttct ttattatacc aaggaaataa gctatcattg
    20341 attaagaaga atatgctaag gagcgaagac gtaatgagaa aaaaagatcg gcatcgtttg
    20401 attactcgat tgttgagtga gcaagatatc cgaaagcagg aagaatttgt tgaaatcctg
    20461 aaaagcaaag gaatatctgt tactcaagct acgatttcca gagatataaa ggaactgaaa
    20521 ctgatcaagg taccggcttc agatggcggc taccgttata gcataccagc tgaaacaaac
    20581 gaagatgtaa atactaaatt ggaaaaacta ttaaaagacg ctttcgtggc agttgaccaa
    20641 atggaaaaat ttgttatttt gaaaacattg ccgggaaatg cttcggcagc agcaaatttg
    20701 attgataaac gttacaagaa agaattattt tcgattatca atgatgacga taatgttttg
    20761 atgattacac gaactgaaga ccaggcagtt ttattgaaga aagacttcct gcattacctt
    20821 taagttgagg agcgaatgtg atgctacaag aaatcagtat tactaacttt gcgattattc
    20881 cggaattgcg gctttctttt cacgaaggaa tgaccgcgct gaccggagag actggtgcgg
    20941 gtaaatccat tattatcgat gctttaggtc tgttagcagg aggaagaggt tcaagcgatt
    21001 atatacgtca aggagcagaa aaatgtgtgc ttgaagggtt gtttgaacta ccaaaacaag
    21061 aaggattctc tgaactgatg gttgaattag ggatcgaaac agatgaggac aatctgattg
    21121 ttcgacggga tatgtcctta actggaaaaa atgtttgtcg agtgaatggt catattatca
    21181 cattagctaa tctgagaaga atcggcagtt atttagtaga tatccaagga caaaacgaac
    21241 atcaggaact gctccagcct gaatcacatc ttgctttatt ggatcgtttt ggagatgtag
    21301 ctttccaaca gaagaaaaag agttatcagc aagaatatat ctcttatcgt gagctggaaa
    21361 aaagggtacg aaaagtgcag caaaatgaaa aatcctatgt tcagagaata gatatgctcc
    21421 atttccagca agaagaaata gctgctgctg atttgcaagt cggagaagaa gagaaattaa
    21481 aagaagaaag agaaaaactt agcaattatc aaaaaattgc tgatggatta gctgcaggtt
    21541 acggagcact gacgaataat gaacaaaaca gtgtagatgg tgttggcttg gccgtttcag
    21601 aactgcaagg gatcgcccat ctagatgtgg agtatgaagc aatttatgaa aatatccaaa
    21661 gtgcttacta tttgctgcaa gatgcaatcg gcgatatgag tcgtcagatt gatttattgg
    21721 agttagatga gagtcgttta gaagaagtta cgcaacgttt agaattaatc cgacagttga
    21781 aacgtaaata cggcgaatcc attgagtcaa tattggctta ctatgacgaa atcaccgaag
    21841 agctagcttc ttctgatttt tcagaaggtc aattggacaa aatgaaagag gaactagagc
    21901 aaaaagagct tctacttcag caacaagccg cagatcttca tgaagcgcgt aaaaaaatcg
    21961 caaaagaatt ggaaaaatcc attctgcatg aattgaaaag tctgtatatg gaaaacactg
    22021 agtttgaggt acgtttctta aaagaagaaa atagacaatt gaatcatgat ggatttgacc
    22081 aaatcgaatt ttacattacg accaatccag gagagccact gaagcctttg gtgaaagtcg
    22141 cttctggtgg cgaactgtct cggatgttgc ttgctttgaa aactattttt tcttctgagc
    22201 aaggtgtgac aagtattatt tttgatgaag tggatacagg tgtgagcgga agagttgcac
    22261 aggccatcgc agataaaatt tctcaaatat cgaaatactc tcaagttttg tgcatcactc
    22321 atctgcctca agttgctgca gtcgccgact atcaatatta tattgtgaaa gcagtgattg
    22381 atggacgtac gcaaacttct gtttcagagt tgaaaacaaa agaaagagaa gaagaaattg
    22441 cacgtatgct ggctggaagt gagattacaa aactcacact cgaacatgca aaagaactat
    22501 tgaaactcgc aaaaaggacc tgagccacct cgcagtggtt caggtccttt tcagcagctg
    22561 atagctatat tttattttta catgacactc attacagcag tcaatacaac agatccaatc
    22621 gttaaaatca gcattgtcca aatgacaaat tttgttactt tagcaaaagt acttgtttgt
    22681 tttttttcac gcattgtttt caacctcgtc attaattagt tgattcaagt gtacaatctt
    22741 ttttccattt ccacaagagg gcttttttat aaaaacttcc ctttttttaa ataataggta
    22801 gttacgatac aaataaataa tgttaataga actagccata taaaggcatc atccctttca
    22861 gcaataggta acttaatgtt cattccatag atactgccga cgattgtcgg tatagttaaa
    22921 ataatcgtta aagaagttaa aattttcata atattattca gattattcga tacgattgct
    22981 gaaaaagtct gattcatttg ttctactagt tttaattgaa tgcgagaagt agtcattgcc
    23041 tgctctgttt ccaccaatac atcacgtaaa tgatttttca aagcatgatt ttcttggaaa
    23101 agtttatttt gcgctagttc tttcaatgca tctaaattag cagaagtcgc ttctctgaaa
    23161 taaacgaggc ttttttggat atccatgatt tgataaagtt gtttgttttc agtggatact
    23221 tgaagtttgg cttccaaagt atcgcattga gattgaagcg tagctaaata actgttgtaa
    23281 gagagtgtga cctgccaaat gaattgaaag aagatattga gattaggcga aatatcactt
    23341 tttggaaagg tctggctaaa gactttcttt aaaaacagtg gttcatgatt cgtaattgtg
    23401 ataagcttct ttttgggcgt caaaataata gagagcgggt atgtatccac ttgaaaataa
    23461 ccgctaggac ttgttttagc aaaaggatat tgtaacaata ataacgctgg ctttgtcaac
    23521 tttgattgat tgaattcttc tgtacgggaa ttttctgcat cgtccaatat ggaagtgatg
    23581 taatctgacg gaatttcaaa ttgatgtgtc agctgatgaa tctcttcttg attaggacgt
    23641 tctacacaat accagatcgt ttggtcagtt tcctttccgg tattacgcag tttctcatta
    23701 tctaatgtaa aatatttgat catgattttc ctcctgttgc ctgtttcttt tattataacg
    23761 gatagggaat tggaatacat ggaatgtgtt tgtaagatat agaatataac tgcataaatt
    23821 taaataaaat gaaacagttg tgtaaagtat atacagtata cttgaagcaa cgataaaaaa
    23881 ggagtttacg ttcatgggca cattcacacc acttatcttg ttttggtata tttacccggt
    23941 aatcgttttg tttggttgcc aatttcttgt aaaattattt tcgttaaatc gtcgttttaa
    24001 aataaaagcc cctgatttgg cgattccttt tttgtggggc ggtatgcatg cattgtctag
    24061 aaatacaggg acaatttcta tcttaccgtt tttctttatt tctgttttgc taatgggaat
    24121 ttgtattgct gttttccaag catattatta tgaagaaatt atttatccgc gttatttcaa
    24181 gatgacttgg cgtttggttt ttctgttgac aatcgtcttg tacgctgtct tgatcattgt
    24241 gaacatactt tcttacctat gaaaaaaata gaaaagctgt aaaaagtctt ttaaaaggct
    24301 ttttgcagct tgatttttaa aaaaatgttt atgaataaaa aaagttgttc ccggatttcc
    24361 cagaaaaggt ggtagaaagt gggggattgt ggtagactaa ttttagttag tggaggaatg
    24421 gggggctttc aatgttgatg ggtgaattcc aacataatat tgatgcaaaa ggacgtttaa
    24481 tcgtgccctc aaaacttcgt gaagaacttg gcgaaaagtt tgttctgaca cgcggattgg
    24541 atggctgtct tttcggctat ccgatgagcg aatgggaaaa tctggaagct aaactgaacg
    24601 aaatgccgtt ggctaaaaaa gacgcacgta cttttgtgcg attcttctat tcggctgcca
    24661 ctgaatgcga acttgataaa caaggacgaa tcaatatacc cagcacgtta agaaattatg
    24721 cagctttgac aaaaggttgc gtagtcatcg gtgtatctaa ccgaattgaa atttgggatg
    24781 agacacgatg gcaagatttt tctgcagcag cagaagagaa ctttgacgaa attgctgaaa
    24841 gtatgattga tttcggacta taagaatagg aagaggctga ctaatgactg aaacatttca
    24901 acactataca gtcatgttga aagaaacggt agatggattg aacatcaaac cggatggcgt
    24961 ttatgtagac tgtacactcg gcggtgccgg acatagtgaa tatttattaa gccaactaag
    25021 ttctaaaggg catctgtatg cgtttgacca agatcaaaaa gcaatcgatc acgccaagct
    25081 gcgtttagct tcttatgtcg aacaaggtat ggtcacattt atcaaagcga acttcagaga
    25141 attagaagaa cgcttgagtg agcatgtaga taaggtagac ggcattttgt atgatctagg
    25201 ggtttcctct cctcagctag atgaagcaga acgtggattt agttatcatc aagatgcacc
    25261 gttggatatg cgcatggatc agtcagctcc tttatccgct tatcacgtaa ttaatgaata
    25321 ttcctaccat gaattagtaa agattttctt ccgctatggc gaggagaaat tttccaaaca
    25381 aattgctcgt gaaatcgaac gagtacgaaa aacaaaacca atccaaacaa ctggtgagct
    25441 tgtcgaaatc attaaacaag tcatcccagc tcccgcaaga agaaaaggcg ggcatcctgc
    25501 aaaaagaatt tttcaagctg tgcggattgc agtaaatgac gaactcggtg ccgaagaggc
    25561 ctctttagaa caggcaatcc ggttattgaa agtcaatgga agaatcagcg taatcacatt
    25621 ccattcattg gaagacagaa ttgtcaaaag tatgtttaaa gaatatagta cggtacaaga
    25681 tctaccaccg ggacttcctg tcatacctga cgaattccaa ccagaactga aagtcattac
    25741 tcgtaaacca atccttcctt cagaggaaga gctaacagaa aacaatcgat cacgcagtgc
    25801 aaagttacgt attgcagaaa aaataagaat gaaagaggag tagaatgatg gcagaattaa
    25861 agaaaatgca agattacgaa tatgatttac ctgaattggt ggatcagccg gaacaacaaa
    25921 tgcctgattc ttcttcgaat caagaaatca ttcttccaca atcaccggca aaacgattaa
    25981 agcagatttc tgctttggaa aaaattgctg tggcaagcgt ggcgattgct attgttgctt
    26041 tatgtgtttt aacagttatg ctgcgcacaa atatcagcgg agctgaaaaa gaaatcacgt
    26101 caatacaaac agaaatcaat gataaaaagc aagaaaaaac gagtttagaa caagaaaaaa
    26161 acgaactttc tcgtactgaa cggattaaac aaatcgcaga agaaaaagga ttgtctatta
    26221 atgacgacaa tttaaggaaa gtgaagtaaa tgagtcttaa aaataaaatt cggcggttta
    26281 tggaaaagaa aaatctaaat ccgatgaaca atcgcaaaaa agtaggtatc attttatttg
    26341 ctacgagtat tggattgttc tttttatttg ccgtccgttt ctcgtatatc gttattggag
    26401 gtcatgttgc tggtacctca ttagctgaaa aaacaaaaca gttgtaccaa ggaagtgaag
    26461 tagtaaaagc aaaaagagga acgatttatg atcgtaacgg tgtggcgctc gcagaagatg
    26521 cctcctctta ttcaattaaa gcgatactct caaaaacata tacctctggg gataaaaaac
    26581 tgtatgttga agaaaagaat tttgataaaa tagcagaaat cctgcataag aatctttcta
    26641 tagacaaaaa agatgcactg aatattttag aggacggcgc aaaaaaagag ctgtatcaag
    26701 ttgaatttgg tagctatggg aaaaatatct cccaagaaac caagcaaaac atcgaagcgg
    26761 acatgaaaaa agaaggtgtc gctggtcttt attttgtcga ccatcaggct cggatgtatc
    26821 caaatggcgt cttttcttct cattttattg gatacgcagt tccagacaaa gatgagaatg
    26881 gcttagttgg taaactaggt ttggagtctg cttacaatga tatcttgagc ggtaaagatg
    26941 gtaagattat ttatcaaaaa gataattttc aaaatcctct gccaggtaca gtggcagaag
    27001 aagaaaaagc tgtcgacgga caagatatct acacgacttt agacagcaga ctacaaagtt
    27061 atttagaaac actgatggat caagtaaatg aagaatatca gccagaagaa ttgacagcag
    27121 tcctgatgaa agcaaaaacc ggagaaatct tggcaatggg acaacgacct actttcaatc
    27181 ctgaaacaat ggaaggattg actggaaaag atgctatctg gcgtaatttc ttggtacaag
    27241 atagttatga accaggatca accatgaaag ttttcactac tgcagctgct atcgaagaag
    27301 gagaattcaa cgagaatgaa accttccaat ctggaaaaat ccaagtagaa gatgctacga
    27361 tcaatgacca tgactttggt gaaaaaggtg tgctgaccat gcgtcaagct ctttcatggt
    27421 caagtaacgt cgggatggtc atactggaac agcgattagg cggacgatgg tataactatt
    27481 tacaaaaact gggatttgga caaagtactc attctggatt agatgatgaa gtaaacggtg
    27541 ctttacctac attaaatatt gtcgaccgag caatgagtgc ttatggacaa gcggtaggtg
    27601 tgacgaattt ccaaatgatg aaagctttca cttctattgc gaataacggt acaatgattc
    27661 aacctcgata tatcagcaaa gtggtggatc cccaaactgg agaagagcga acaactcaaa
    27721 cagaagttct agggcaacct ttttcaaaag aaacaacaga aaaagtccgt gaatatatga
    27781 gagatgttgt cgaaagcgaa aactacggta gtgcttatgg tgtatatagt gtacctggat
    27841 acaatgtatc ggcaaaaaac ggaacagccc aaatcgcttc tgatacaggg ggatatcaaa
    27901 caggagatac agcctacctg tattcgattg tggagatggt tccttctgaa gatccagact
    27961 atgtcttgta tctaacaatg aaacacccta agacatatga tcgaatggca ttggctaaaa
    28021 tcgcaaatcc attgatgaaa cgggcaatgg attttaaaga aactgaagaa gattcagata
    28081 cagaaacaaa aacagaaaaa gtatccgtag ccgattatcg taatcttgaa gcggacgttg
    28141 cagcagctga tgctcaaaaa agtggactgc aacctgtagt gatcggtaat ggtaaaaaag
    28201 tacaaaaaca atctactgcc aacggtgatc agttgatctc tggagaaaaa ctgatcctat
    28261 atactggtgg tgataagctg atgcctgacg taacaggatg gtcaaaagca gatatcatga
    28321 agctggggaa aatcctcggt attgaagtta gttttgatgg agatggttat tgtgtcaaac
    28381 aagaacttgc tccatatgaa aaaatcactg aagacaaact tagttttact ttagaagaat
    28441 aagaagagaa ataggagaga aaagagcatg gaatggacac aagcattgat tcccattgtc
    28501 agcagttgtg caatgacaat tgccgcaatg ccgctattta ttggttattt tcaaatgaaa
    28561 aagcaaggtc aggcgattcg tgaggaggga ccaaagtggc acaatagcaa agccggaact
    28621 cctacaatgg gcggcttagt gtttctgatc ggctctattt tgacaggaat ttgggttgga
    28681 gcttggcaaa aacagctaac tcctactctt ttcatcttgc tttttgtatt agcgttatac
    28741 ggagtaatcg gctttcttga tgattttata aaaatattca agaaaagaaa catgggactg
    28801 aactcgaagc agaaattgat tggacagatt ctagggggaa tcatctttta tcttgtttac
    28861 cgttctgaag gttatccagg tgtattgaat ttcttcggac tagatctacc tttaggaatc
    28921 atttatggta ttttcgctct cttctggctt gttggatttt caaatgcagt aaatctgaca
    28981 gatggcatcg atggattagt tgctggctta ggaagtatct cttttgcagc ttacggtcta
    29041 attgcttggc atcagcatca gtatgatgta ttaatcattt gcttaagtgt tttaggaggt
    29101 ttactgggtt tcttcgtcta caacagaaaa cctgccaaga tttttatggg agatgtcggt
    29161 tcattagcgt tgggcggact tttagcggca atttctataa tgctaaacca agaatggaca
    29221 ttgcttttga ttggtttgat ctatgttatg gaaacagcaa gtgtgatgct tcaagttact
    29281 tcctttaagc ttacaggaaa acgaattttt aaaatgtctc caattcatca tcactttgaa
    29341 atgtgcggat ggtcggagtg gaagattgat acagtttttt ggttgatcag cattgtgact
    29401 tcgttgatca cattatggat cgtttggtag gagatgacgg gaaatgaaaa aaatatcaac
    29461 atatgaaaat aagaaagtgc ttgtcttagg actcgcaaaa agcggagtaa gtgcggccaa
    29521 attattacat gaattgggcg cacttgtcac cgttaatgat ggaaaacctt ttgacgaaaa
    29581 tccggaagca caagagctgc tttctttagg gataaaagta ataacaggta gtcatccaat
    29641 cgaattgttg gatgaagaat tttcattgat ggtcaaaaat ccagggattc cctacagtca
    29701 tcctttagta gcaaaagccc aagaaatggg tattccggta attacggaag ttgagctagc
    29761 ttacgaagta gcggaatgcc caattattgg gattacagga acgaacggaa aaacaacgac
    29821 aacaacgatg acaggactac tactgaatgc aggagcagat caaggaattg cacgtttagc
    29881 gggtaatatt gggtatccag caagtggtgt agcgcaagaa gcaaaatcag aagataaaat
    29941 tgtgatggaa ttgtctagct tccaactcat gggaatcaca gatttccgcc ctcatatagc
    30001 ggtgattacc aatatctatg aagcacatat tgattatcat ggtacaagaa aagaatatgt
    30061 caaagctaaa tggaaccttc aaaaaaacat gacagaaaaa gattatctga tccttaactg
    30121 gaatcagtca gaactacaag aattagcgca aagaacaaaa gcacgcgtac tgcctttctc
    30181 gacaaaagaa gtgttggaag acggtgtgta tgctgacgat tactcaatat actataaaaa
    30241 agaaaaaata atggaaatat ctgaacttgg cgtaccaggt aagcataatg tagaaaatgc
    30301 actagctgct atttctgtag caaaattgta tggtatatca aatgaagcta tacgagaaac
    30361 gttgcatttt ttccatggcg taccacatcg tacgcaatat gtaggagaaa tccaaggcag
    30421 aaaattttat aatgattcaa aagcaaccaa tattttagcg actaaaatgg ctctaagtgg
    30481 atttgaaacg tctaaggtcg tgttattggc tggcggatta gacagaggaa atacatttga
    30541 tgaattaatt ccttcattaa aaggaatcaa agcaatggtc gtatttggcc aaacaaaaga
    30601 aaagttgatg gatgctggaa aaaaagcagg catcgagaca atagtaacag ccgattctgt
    30661 agaacaagca gttcctttag cattagaaaa cagtacagat ggtgatgtag ttttattatc
    30721 accagcgaat gccagctggg atcaataccc aaattttgaa actagaggaa acagattcat
    30781 ggaagctgtg aaccgattaa aatagaaaaa gtagaagcga gcgataaaaa atgaaaattt
    30841 tggttacagg cggaggaaca gggggacata tttatccagc tcttgccttt gtcaattatg
    30901 taaagactca agaaccgaat gctgaattca tgtatgtggg agcaaaacga ggtctagaaa
    30961 acaaaatcgt ccctgataca ggaatgcctt tccatacctt ggagattcaa ggatttaaac
    31021 gaaaaatttc catgcataac atcaagacgg tccgattgtt tcttaagagt atccgtcaag
    31081 caaaaaaaat cctcagtgat tttcaaccag atatcgttat cggtactgga ggatatgttt
    31141 ccggagcggt agtgtacgct gcagccaaaa tgggtattcc aacagttatc catgaacaaa
    31201 acagtgtacc aggtgttacc aataaatttt taagccgata tgtggatcgg atcgctcttt
    31261 catttaaaga tgcggcttcc ttttttcctg aaaacaaagc agttcttgta ggtaatcctc
    31321 gagcacaaga agtagcagat actaaaaaat cagatatttt gaaaacattt gatttggatc
    31381 cagaaaagaa aacagtcttg atttttggcg gtagtcaagg agcacttaaa atcaatcaag
    31441 cagtatcagt ttttttaagt gaatatattc cttcttctta ccaagttttg tatgcttcag
    31501 gagatagata ttatgaagag atcctaaata aaataaatcc tgaaatacaa aatattagtg
    31561 tccagcctta tatcaaaaat atggcagagg tcatggcttc cagtgatctg ctggtggggc
    31621 gtgctggtgc aacttcgatc gcagaattca cagctctggg gttaccggca gtgttggttc
    31681 ccagcccata tgttacgaat gatcatcaga ctaaaaatgc aatgagtctt gttcacgcag
    31741 gagcagcaaa aatgattgca gataatgaac taacaggtga atcactaagt cagacagtca
    31801 acgaaatcat gggtgatgaa gaattgcaaa agcaaatgtg ccgagcttct aaagaacaag
    31861 gaatccctga tgcttcaaaa cgtctttatg acttagtaaa acaaataatc aaaaaataaa
    31921 ggcggtggtc ttagtgagta agaaaaaaga accaaaaaaa ttgtctcatc cgtcttctga
    31981 tgaaaacaat ttgactcctt ggcaaaaggc taaccgaaaa tatctggcag aacaagagat
    32041 gaaaaaccaa gagccaaaaa aagaagacat aacattagat acaccccttt cagattctga
    32101 agaagaaaaa acggttcaag aggaacagca agaagaaagc cagtccgaga aaaaaatcga
    32161 attttttgaa gagatcaagc aaaagaaaaa cggtggacca tataacggtt cttttttgaa
    32221 ccggctgccc aatttaaaaa gtcaacggca caaagtacta tttcgtcggt tagctttgat
    32281 catcactgta ttgggtatac cgctgatttt tttgatctat tatgtttctc cttatagcaa
    32341 attacaagca gttgcagtat caggcaataa gacagtcaac agccaagaaa tcatctctga
    32401 tacaaaattg tcattaggag aaaatgtttg gggacagtat tttcatcgat ctacttatat
    32461 cgatcgcctt aaaaaagcac agccaagaat cgaaacagca aatatacatt tcaaagggat
    32521 gaatgaattc gatttggatg taacagaata taaagagatt gctttgatcg caaaaaacaa
    32581 tcaatactac ccagtgattg aaaatggaac agtattagat gaaaaagtag caaatcctac
    32641 aaaaaatctc cctatcttgg aagagttcaa agactctgca aaaatcaaag aattaacaaa
    32701 gcaatacaat cagttatcat cagaattaca aaaagcaatt tctgaaatca agtacacgcc
    32761 aagagcaagc aataaaaatc tgatccaatt gaatatgaat gacggcaatc aagttattgt
    32821 aaatatcaat aatcttgcga accaaatgaa atattattcg caagtagcca aagatatgga
    32881 tgaaaaaggt gtgatcgaca tggaagttgg tatcttctcg catccttacg gtagcactca
    32941 agaaacggaa gaatcgggcc aatcggaaga aacaactgaa agtgaagaaa atgcggatca
    33001 aacaaacgaa tcaagtgaag aggcagaaac gcaaaaagca caaacacctg gatcgtctag
    33061 cacaaacgga tcatctgatt caaatgattc aagtgaaaca agtcaaatag aagataatcc
    33121 aagtacaact ccgtcaagtt taaatgaaaa ttgataaaac aactttaaaa agtaagaatg
    33181 tactttctaa aaaccagtaa tttatggtag aattggaagc agtgaatata tctattaata
    33241 agtatatttt aaaacggacg ctagttgttc gcttgtatat ggaatagtaa gtaggaggga
    33301 atcccattca tggcaaaaac aggaatgtat gtaggccttg atattggaac gacatctgtt
    33361 aaagttgtcg tggctgaata tatcgacagc caaatgaata ttattggagt aggaaacgca
    33421 aaatcagaag ggattaaccg aggcattatc gttgatatcg ataaaacggt ccaagcgata
    33481 caacgagcag tacgacaagc agaagaaaaa gcaggtatcc agatcaaagg cgtaagtgtc
    33541 ggtttgccag caaacatgct tgaagtagaa aattgccagg gaatgattgc agttaacgga
    33601 gattcaaagg aaatcaccga tgaagatgtc agaaatgtgg cttcagctgc ccttgttcgt
    33661 tcaatacctc ctgaacgtca aatcgtgtca atattgccac aagattttac tgtcgatggg
    33721 ttcgagggaa tcaaagatcc tcgtggaatg atcggcgtaa gattagaaat gtacggtctg
    33781 ttgtttacag gacctaaaac gatcatccat aacatacgta aatgcgtgga aaatgccgga
    33841 ttaatcgtca acgaaatggt gattactcct ttagcgctga ctgaatcaat tctttctgac
    33901 ggagaaaaag attttggtac gatcgtcatt gatatgggtg gcggccaaac aacaacggct
    33961 gttatgcatg ataagcaatt gaagtttacc aatcttgacc aagaaggtgg cgaatttgtc
    34021 actaaagata tctctattgt attaaatacg tcatttaaca atgcagaagc gttgaaaata
    34081 aactacgggg atgcataccc agaacggaca tcaccagatg aagaatttcc agtagatgtg
    34141 attggtcagt cagaaccagt gaaagtcgat gaacgctact tgtcagaaat tatttcagct
    34201 cgaatggaac agattttcaa caaagcaaaa gatgcattgg accaaatcga agcattagag
    34261 ttgccaggtg gcgtcgtatt gacaggcgga gctgctagtc ttcctggtgt tgtggacttg
    34321 gcacaagaaa tcttcggcgt aaatgtaaaa ttgtatgttc caaatcacat ggggctgaga
    34381 aacccggtat ttacaaatgt aataagtatc gtggattatt cagcaaactt gagtgaagtg
    34441 tatcaattgg caaaaattgc tgttacgggt gaaacctcag cagctcgtca aatggtagtt
    34501 gaacaagaaa caacaaacac atatgagagc tatgaagcac ctgaagaaac aatctacgat
    34561 gagccggaac caaaagagtc cggagaaaat gtaaaaaata aaatcaaggg tttctttaca
    34621 aatatttttg attaattcat cctgggagga ataagatatg gaattctcaa ttgataataa
    34681 tatcaatgac ggcgcagtaa tcaaagtcat cggagtcggt ggtggcggtg gaaatgctgt
    34741 aaaccgaatg attgaagaaa acgttaaagg tgttgaattc attacagcca acacagacgt
    34801 gcaagcatta aaaaattcaa aagctgaaac agttatccaa ttaggcccca aatatactcg
    34861 tggtttaggt gctggttctc agccagaagt tggacaaaaa gcggcagaag aaagtgaaca
    34921 atccttacgc gaagctttag acggtgcaga tatgattttc atcactgcag gaatgggtgg
    34981 aggaaccggt acaggtgctg caccaatcgt tgcaggtatc gcgaaagaat tgggcgcatt
    35041 gactgttggt gtcgtgacac gtccattcac ttttgaagga ccgaaacgtg gccgttttgc
    35101 tgcagaaggt atcgctcgtt tgaaagaaaa tgtggataca ttattaatta tctcaaataa
    35161 ccgtttatta gaagttgtag ataaaaagac accgatgctt gaagctttcc gtgaagcaga
    35221 taatgtttta cgacaaggtg tacaaggtat ctctgatttg atcacagcgc caggttacgt
    35281 taaccttgac tttgctgacg tgaaaacagt gatggaaaac caaggtacag cattgatggg
    35341 gatcggtgtg gctagtggag aagatcgcgt agtagaagca acgaaaaaag caatctcctc
    35401 tccattgctt gaaacatcta ttgatggagc tgaacaagta cttcttaaca tcacaggcgg
    35461 tttggatatg acattgttcg aagcacaaga tgcttcagat atcgtagcaa atgcagcaac
    35521 tggcgatgta aatatcattc taggtacatc tatcaatgaa gaaatgggcg atgaaatccg
    35581 tgtgacggtc atcgcaacag gtatcgatga atcaaaaaag gaacgcaagt ctagccgacc
    35641 agctagacaa gcacaaatgc aatcacctgc tcaaaagact gtattagata tggatcaagc
    35701 taaaccaact tcttcagaag aagaaaacag ttttggcgat tgggatatca gacgtgaaca
    35761 aaatgttcgt cctagagttg atgattccaa ttttgacaat atcgaaaaaa aagaatttga
    35821 tacattcaac cgcgaagaaa caaaatctca tggcgatgat gaattaaata cgccaccgtt
    35881 tttccgtcgt aaaaaataaa ggagaggaag agcacatgat tgctgacaac ttgcagaaaa
    35941 tcaagcaaga aattcaggat tcgtgtgctc ttgttcatcg tgatccgaaa gaagttacat
    36001 tagtagctgt aacaaaatct gttgaatccg ctcaagctga agaattggtt agagctggcg
    36061 ttatgaattg tgcagaaaac cgagtagata aattattaga aaagaaacaa gatttatttg
    36121 attttcctga aataaagtgg catttaattg gtaatttaca gcgcagaaag gtaaaattga
    36181 ttgtaaatga aattgattac tttcatgcgt tggacagttt aagattagca gaagaaatcc
    36241 agaagcggtt agagaaagaa ttatcctgtt ttattgaagt aaatgtttct ggtgaggaat
    36301 caaaacatgg aatttcacca gatgagcttt taccgtttgt ggaatcttta gctgcctgca
    36361 caaaaatcaa ggttgttggg ttgatgacta tggcgcctaa agaagcttct gatgagcaaa
    36421 tccataaagt gtttggtacg ctgaaagaat tgcgtgatct tgttcaagca aaaggttatg
    36481 cccatgcacc atgtagtgaa ttaagtatgg ggatgagtca agactttcaa attgcgattg
    36541 aagaaggtgc aacatttatc cgtgtaggaa cagcgctatt caaagacgaa taaaggagga
    36601 acaaaaatgc cgcttttaaa cagaggagca attgctaatt ttttcggatt agctgatgag
    36661 gaagagtacg aataccaaga ttatcctgaa acaacaaaaa aggctgtaca gccaactaaa
    36721 aaaaatacaa cacagaatca atcaactcat tctgtatctg ctgcgaatac tcatgaaaaa
    36781 caaaaaacgg cgacttcacg tcctgtaacg catgaaagta ttcagccggc tgaaaagaaa
    36841 aaagtaattg aactacatca atcacctaca aaaactgtag cgaaagaaag aacagcttca
    36901 agaacctcta cacaacctaa agcaaatctt gctggaaaaa tcactgtaat ggaaccaaga
    36961 gcttattctg aagctatggc gattgctaaa cgaatcattg ctggagaagc aaccttagtg
    37021 aattttcatt tagtagaaga aaaacaagcg agaagaatcg ttgattttct aacaggaaca
    37081 gtttatgcac tagatggaga tatcaaacgt gttggagatg agatttttct ttgtacaccg
    37141 actgggatgg agattgacag ttcaaccgct caatcttttg cagatgcgaa tttgtttgat
    37201 ctgtaagagg aggaactacg atttatacta ttgcactatt acttcattta cttcaacggg
    37261 cagcctattt ctatagcatt atcttgatgg tatatgcatt gctctcttgg tttccaggcg
    37321 ggtatcaatc agggctagga cgattattag ctaagatctg cgaaccgtat atcagtttgt
    37381 tcgatcgtct gcctttgagg attggaccct tggatttcac aattttaggg gcaatcctga
    37441 tcttgaactt agccgtacaa ggtttagcac agatcgttat atggataact gtccatttag
    37501 tttattgatt gaagcaacaa aatctttatt ctcagaatag ataggagggg aaaattttgg
    37561 atgcaaatgt ataccagcac tttcgggcag atgaacgtac atttatcgat tcggtaggcg
    37621 attggattga acaggttcaa ggacaatatg caccctatct gaccgaattt cttgacccaa
    37681 gacaatccta tattttagaa acactgattc gacaaagcag tgatttgcgt ttcatgtttt
    37741 atggcggata tgaacaggca gaaagaaaaa gatgtttgat tttccccgat tattacgaac
    37801 ctgtagagga agattttgaa acagcattgt atgaaatcca ttatcctagc aaatttgcca
    37861 gcctaagtca tgggaaaatc cttggaactt tagtgggaac ggggatcaaa cgctcttact
    37921 ttggcgatat catctcggac ggtgatcgct ggcaagtatt tattgcaaat gaggtggatc
    37981 attttgttgc tgcacaagta acaaagattg gaaaggttgc tgtacgcttg gaaccaatca
    38041 aatacacaga aatgattatg ccgaaagata gctggacaca agaaagagga acagttagtt
    38101 ccatgcgact agacagtgtg atctctgaga tttataatat ttccagacag cggtccaagc
    38161 agctgatcga agctggtaaa gtaaaagtga actggacaga aaatacaaga cctgattttg
    38221 tacttgaact attagatatt atttcagtca gaggttatgg cagagtccag atccaagaac
    38281 ttgaggggaa gacgaaaaaa gaaaagtaca gggtactgtt cggtgtatta cgtaaataaa
    38341 ataaaaaggt ggtttactca tggctttaac tccattagat atccaaaata agacattccc
    38401 gacaaaaatg cggggatata atcaagacga agtagatgat ttcttagatt tagttgttcg
    38461 tgactatgag gaattgactc aaagaaatcg agaattagaa aaagcagtca aacattctga
    38521 agaaaaactt gagtacttca atgaattaaa agatgctttg aaccaatcaa tcatcgtagc
    38581 tcaagatacg gctgataaag taaaaacaag tgcaagcaag gaatccgaag tgatcgttac
    38641 ttctgcacaa aacaaagcag atgagttagt tgcgaacgct gaaaaacgtg cacatcaatt
    38701 aacaactgat gcagaagaaa aagcaagaaa aatcttgaca gatgcaacag aaaaagcaag
    38761 acagcttgcg acagaaactg aagatcttaa gaaaaaaaca cgagtattcc atcaacgtat
    38821 cagcttgatg cttgagtctc aattagaaca agtgaaaagt ccggaatggg atgaaatttt
    38881 acaaccattc tccagctacg tgactgacag ccatgaagtg atcaaagaag ttttagcaaa
    38941 acagcttgac aatgaaaatg atactgatgt aaactcagac acaggagtca tagaagcacc
    39001 tatgacggca tttgatacac aggctattga tatctcgatc gtgcctggtg aaaatgaaaa
    39061 cgagtagaac agaaaagcac tttgttattc ttaaagcgag ccgatgagag tggaagttcg
    39121 gtaagacact gcatggtgaa agatcctcta cttgagacct gcttttccga aattagagta
    39181 agaagcagcg gctgacctcg ttattggtca cagagagttt gcactagata gatgcaacta
    39241 aatttgggtg gtaccacggc atgttcgtcc cttgcggctg aacatgcttt tttgtgtccg
    39301 gaaaacaaat aaaggagtgt atggttgaat gaaaatgaaa gaaaccttgc aattaggaaa
    39361 aacaaaattc cctatgcgtg gtaatttgcc aaaccgagaa ggcgagtggc aaaaagaatg
    39421 ggaagaaaat cgaatctacc aaaaaagaca agagttgaac gaaggaaaac ctagctttgt
    39481 tcttcatgat ggacctccat atgccaatgg taatatccat ttaggacatt cattgaataa
    39541 aatcagtaaa gacattatca ttcgttcaaa atcaatgtct ggctatcgtg caccatatgt
    39601 tccaggatgg gatacacatg gattgccgat tgaacaagta ttggctaata aaggcgtgaa
    39661 acgtaaagaa atgtccatgg cagaatactt ggaaaaatgt cgtgaatatg cactttccca
    39721 agtagataag caacgagcag atttcaaacg cctaggagtt gcaggagatt gggaacatcc
    39781 ttatgttaca ttagatcctt cttatgaagc tgcacaagta cgcgtatttg gtaaaatggc
    39841 tgaaaaaggc tatatttaca aaggattgaa accaatttac tggtctccgt caagtgagtc
    39901 ttctttagcg gaagctgaaa ttgaatataa agatgtaaaa tctccatcta tttttgtagc
    39961 tttcaatgtc aaagacggca aagatattct tgatgaagat acagcttttg tcatttggac
    40021 gacaacacca tggacattgc cagcaaacca aggtattgct gtaaatccaa aatttacgta
    40081 tgtattggtg gaagccgatg gccgtaagtt tgtcgtagcg aaagatttgc tagaaacagt
    40141 ccaatcagaa atcggttggg aaaatgtaac agttctaaaa gaatttgctg gacaagaaat
    40201 ggaatatatg actgcaagtc atccattcta tgaacgtgaa tcacttgtga ttttaggcga
    40261 tcatgttact ttagacgctg gtactggatt agtccatact gctccaggac atggggaaga
    40321 tgactatatc gctggtagca aatataaact atctgtagtg tcacctgtag actcaaaagg
    40381 attgtttaca gaagaagcac ctggatttga aggtatcttt tatgacaaag caaatcctat
    40441 gatcactgat ctattaaaag aaaaaggagc tttgctgaag ctagatttct ttactcatag
    40501 ttatcctcac gattggcgga cgaaaaagcc tgtgatctat cgggcaactc cacaatggtt
    40561 tgcatctatc gataaattcc gtcagaatat tttggatgaa gtagaaaaag tagagtgggt
    40621 tatcccatgg ggtaaaacac gtttgtataa tatgatccgt gaccgcggcg attgggtaat
    40681 ttcccgtcag cgtgcatggg gagtaccact tccgattttc tatgctgaaa atggagaagc
    40741 aatcatcaca ccagaaacaa tcgaacatgt ggcaaaatta tttgctgaac atggatcaat
    40801 tatctggttt gaaaaagaag cgaaagaact tcttcctgaa ggatttaccc acccaggttc
    40861 tcctaatggc gaatttacaa aagaaaaaga tatcatggat gtctggtttg attctggttc
    40921 ttctcatgaa gctgttttac gtcagcgacc agaattatca ttcccagcag atatgtattt
    40981 agaaggatca gaccaatatc gtggctggtt caactcaagt atcacgacta gtgtggctat
    41041 caatggtgta gcaccataca aatcagttat ttctcaagga tttgctttag atggtgaagg
    41101 ccgtaagatg agtaaatctt taggaaacat cattgcacca gaaaaagtga tcaaacaatt
    41161 tggtgcagat atccttcgct tatgggtatc tagtgtagat tatgaagcag atgttcgtgt
    41221 ttctatggat atcttaagtc aagtggcaga agtatatcgt aaaatcagaa atacgatgcg
    41281 tttcttatta gcgaatacag aagatttcga tcctaaaaaa gatactgttt cgtacaatga
    41341 tttacgttct gtagacaaat atatgaccgt tcgattgaac caagtaatca aagaaatccg
    41401 agaagaaggg tacgacaaat ataacttcat gcatatttac cgtacagtta tgaacttctt
    41461 gacggttgat ctatcgtcat tctacctgga ttttgctaaa gatgtagttt atattgaagc
    41521 agaagatgat tatcaacgtc gttgcatgca aactgttttc tatcaaacag ctgtagcttt
    41581 gacaaaatta ttgactccta tcattccgca tacagcagaa gaaatctggt cattcctgaa
    41641 agaagaagag gagtacgttc agttaagcga gtttcctggt tatgaagagt ttgcaaacca
    41701 agaagaattg atggatacct ggactgcttt catggacttc cgtgataatg tcttaaaagc
    41761 attggaagaa gcaagaaaca gcaaattgat tggtaaatct ttggaagcaa aactaactgt
    41821 ttatccaaaa caacaagttc gtgaaatgtt aaaagctttg gatgcagata ttgcacaatt
    41881 attgatcgtt tcaccagact actttgaagt tatggcagca gatgaacaag tgccagataa
    41941 cgctatggta tttgatgatg ttgctattac agtggaaaaa gcagacgggg aaacttgcga
    42001 tcgctgtcgc caagttcgta aagatgtagg agtagatgaa aaacttcctc atctatgcgg
    42061 tcgttgtgca aaaatcgttg aagacttcta tcctgaagct gtagctgaag gattcgaaga
    42121 aaagtaaaag ggaaaagggg gtgtgacatg ttctttgtca cacccccttt tgttatcaat
    42181 cgtttaattt tccacgttca cgataccagt tatctggttg ccagatccat tcgaatccgt
    42241 ctttttcaag tagttcgaaa gctgctttag gtcccattga tccagctttg tagttgggaa
    42301 aatccggttg tgtttggtcc caagcttttc gaatgacatc cacgatatgc caagattgag
    42361 cgacttcttc ccaatgagag aagttcgttc catcaccatt caacgcatct aatagtaatt
    42421 tttcgtaagc ttctgggcta ttttcaacca tttctgcact atttctaaat tccaatttga
    42481 ttggttccgt ttcaaatcct tgccctactt ctttcccatt caaggataaa gaaaatcctt
    42541 cagttggctg aatatagatc gttagaacat taggtggaag tgtcgtatcg tcacatggtt
    42601 catcgacaga tgttttgaat acattaacag gaacttgttt gaatacgatg ttgatccgag
    42661 tccctttttc agtcaaacgt ttgcctgtac gtacatagaa aggaacacct gaccaacgga
    42721 agttatcgat agtgaatttg cctgcgacga atgtttcagt ctctgattga gcatccactt
    42781 tgtcttcttc gcgatagccc ttgaattgta caccatccaa ttcaccttga gcatattgcc
    42841 cgcgaacgaa gttttctaac acttcttctt cgttataaat gcggatagct ttcaatgctt
    42901 tgatcttttc tgtacggatt tctttttcgg aaaatgctac tggcggttcc attgcaagta
    42961 atgctacaac ctgtaaaata tgattttgca ccatgtcttt aagtgcgcca ctatgatcgt
    43021 agtagccccc gcgatcttct acaccgaggt tttcgccaaa agtaatttgg atattgtcga
    43081 tatagcgatt gttccattga gcctcaaaaa tattattggc aaaacggata gctgagatgt
    43141 tttggatcat ttcttttcct aaataatgat caatcctgaa aatatcttgt tctgggaata
    43201 cttctctgat ttgctggttc aattcaaaag cagactggta atcagaaccg aacggttttt
    43261 ctatgatcaa acgattgaag ccattttttg tcataatatt ttgtgatttc aaatgagaaa
    43321 cgatcgttcc aaaaaattga ggtgccatag ccaaataata gacacggtta ccgtttaatt
    43381 gatagtgtcc atctaatcgg tcagacaatt cttttaatgt ctgataatgc gttgaatcat
    43441 ttacatcatg ggattgatag taaaaatggc ttgcaaaggt agttgcttct tcatctgaag
    43501 gattcaatgc acggattgtt tctttgacaa cttcacggta gtgctcatcg ctccagggac
    43561 ggcgagcggt tccgattaca gcaaaatgct cgcctaaatt tccttttctg taaaggcgga
    43621 acaaagacgg gtacaattta cgctgggcta ggtcacctgt accgccgaat atcgtaaaca
    43681 atacattttt ttcattagac atgtggatac actcctttat attttatcga caaaaatcgt
    43741 gctggccgcc ttaaagctga cggggatttt ctttctatca ttttggatag tgatcggtcc
    43801 ttcataagct gcgatgtcaa cgatctcata ttcttcgttg atattcaggt caatagtgac
    43861 taagtaatcc aacaattctt tttcatcaag cactcgtgcg atacgcactt ttgtaccgac
    43921 aggataggac tccaatgttt gtcggcgttc ttcatggaca acttcctctt ttttaggaat
    43981 cgatcctcca tgaggacaat gttcaggatg attcaaatag ttttctaaat gatctgcaag
    44041 atgttccgaa gtcacatgtt ccaatacttc agcatcatca tggacttcat tccagctata
    44101 gtctaaatgt tcaactaaaa aaacttccca taagcgatgt ttgcgaatca gactgctagc
    44161 tttttgcagt ccgcttgcag tcaattgtac accttgataa ggggaatgtt ctacaagctc
    44221 ttctttcaca agtttagaaa tcatttcact gacagatgca gctgagacat ctagactaga
    44281 tacgatttgt ttattgttga ctttcgttgt atctccacct agttctaaaa tgattttcaa
    44341 ataatcttct cgatttggtg tcatattcga tcatcctttc atggtaaaaa accatcatta
    44401 gtaagtgtaa caaaaaaaac gttaattgac tatttttcta aataaaaaac aaaggatttt
    44461 tagacgtttt tacaacaaaa aacgaaagat gatagagaag actcattctt tcgtttataa
    44521 aggatatata tatacattgg gagggatcta tttttcagat aacaacttat atgaagtgtc
    44581 tgcagattcg tcataaagag tttgttcggg ataagaaaag ataagataaa aaccatactg
    44641 atcacgtacg actaaacgat attcatctat ttctaccaca gatgtgccaa gagactgacc
    44701 gttgatcaaa aacgtgtaat cagatgtaat ctgtaattgt tgtttttgct ggctatcact
    44761 aaagaaccat gtaccttgat actttgagag tccttctgac ttttccgctt gttttttctt
    44821 tttcaagtaa ataaaacttg agagaacggt aatgagtgaa ccaagaaaat agatcatttt
    44881 ttttatcacc aaatcactcc tttatgagaa aataatcgca tagacatatt ctttcttctt
    44941 tatttcattg taccgaataa aagctaaaat ttctctaaga ctttcgactg gtttttttac
    45001 tgtttttcta caaattatga taggatacat agtaggaaga aaggtgacat aatttgaagt
    45061 atcagcaagt ggaaatagct ttttttattg accgcccgaa tcggtttata gcacattgta
    45121 tgaaaaaaga cggagaaata attattgcgc atgtcaaaaa tacaggtaga ggaaaagagg
    45181 tcctgatacc aggcgctgaa gtggctattg tatttgctcc tggacctaaa cggaaaaccg
    45241 cctatgatct gattgctgta aaaaagaagg gtgactggtt taatatcgat agtcaattgc
    45301 ctaatcggct ggcaattgat ggtattttag acggcacgat ccttttacca ggattaactg
    45361 gtccatttac ggtatacaag cgtgaagtaa cttatcttca ctccaaattt gatatttatt
    45421 tagagacggt ggtcggacaa aaagcatttg ttgaagtcaa aggtatgaca cttgaaaata
    45481 aagcgattgg tgcttttcct gatgcgccca cactcagagg attgaaacat ataggagaat
    45541 taacgtatgc cgcacaagat ggttatgccg cttatgtctt gtttattgct cagttcgaac
    45601 atcttcatct ggcaacgatc catgaagaaa tgcaaccagc actagctgat atggttcgac
    45661 atgcgcagca aagcggagtt caaatcctcg cctataattg tcaagtatcg gcagatgagg
    45721 taactgtcaa gcaggccatt ccatttgatg tagatatccc ttttgaagat cctatacaat
    45781 agaaagaagg taaaataatg atcaacttag gtattatcgg aacaaattgg attactcatc
    45841 aatttgtcca agcagcttta agcacaaaaa aatatgaatt gacagcggta tattctagac
    45901 atttggaaaa agcacaggaa tttggttctc attatgaagg ggatatcgaa ttcgcaacag
    45961 atttgaagac ttttttcaat attactcata tggacaccat ttatattgct tctccgaata
    46021 gtcttcactt tagtcaagct aagcaagcaa ttttggcagg aaaaaatgtg atagttgaaa
    46081 agccagcttt ttcgacgcca aaagaaatgg atgaaatcat ccatttggca aatgaacaca
    46141 atgtgtattt ttttgaagca gcaagaaata tccatgaatc aggttttaaa aagattgccg
    46201 attacttacc attaaaagac gagataatcg gtgcaaattt cagctatatg aaatattctt
    46261 cgcgctatga ccaagtatta gatggagaag aaccgaatat cttctcgact catttctcag
    46321 gtggtgcatt gatggattta ggtgtttacc ttgtatatgc agctgttgct tggtttggaa
    46381 tgccaaaaga agtccattat ttcccaagaa agatttctac tggtgtagat ggtcttggat
    46441 gggggatctt acgttatgag aattttgatg tatcgatcca gccaggtaaa atcggtgatt
    46501 cttatttacc gtcagagatt tattttgact caggaacgct agtgatgaat ggagtcaatg
    46561 caattgaaca agcacaattt catgatcgcg cgcataaaga aatcattgat ttggcggtaa
    46621 cagctgcaga aaatccaatg gcagatgaag cagaggattt tgccaatgtc atggctcatc
    46681 cgaaagatcc agcttgggga ttgttgtatg aagagtggat cgagctagct agaaatgtga
    46741 accaagtgat ttatgattta agaaaaaatg gcggcatcaa attcgatgca gataatgagg
    46801 aagattttta atgaacgaat ttgtacaagt atttcccgaa aattggcaga aaaaatatga
    46861 ggataaaggg tttatctcac cttctcttat tcaacgagca gtttttcagc cactaagtaa
    46921 taaacaaagt atagtagcgg tttctcctac aggttcaggt aaaacactgg cttatctttg
    46981 gcctctacta ctaaacgtgg aaccaggtga agcaagtgcg ttggtcattt ttgcttcttc
    47041 tcaagaacta gctattcaag tagcagatgt tgcacgtgaa tggggaaaag acaaagagtt
    47101 gaaagtccaa tctttggtgg gcggagccaa cgtcaaacgt cagattgagg gtttgaagga
    47161 aaaaccagaa atcttgattg gtacaccagg aagaatattg gaattgatga aagcaaaaaa
    47221 aataaaagca catcaagtga aaacaatggt atttgatgaa gcagaccagc tatttgacgc
    47281 tgggaacagc caaatcattg atcaaattct tcatcaagct ccaacagagt atcagctagc
    47341 ttttttttca gcgacagcag atcgttccct tgaaagcatt gaaaaaatca ctgggaaaac
    47401 gtttgagaca attgacgtga cagcagagga cgattctcga aaaggactga gacattactt
    47461 tttacgtgta ccgatacgaa agaaagatga ctacttgaga cgtctcacgc atatagaaga
    47521 ttttcatggt ctgattttct ttaatcaact gactgaatta ggcgtaatgg aagaaaaatt
    47581 gctgtataga ggtgtaccgg taggaagtct ggcctctgat cagaacaagc tactccgaaa
    47641 agcagcgcta gaacaattca aaaagaaaat ggtcagagca cttttgacca cagatgtggc
    47701 ggctagaggt ttagatatta caggtcttcc ttatgtagtg aatgcagaag ttccgttaag
    47761 tgaagaagct tatttgcatc gttcgggaag aactggtcga atgggtaatg aaggaatagt
    47821 aattacactc gtacaagata ataatctaag agatttaaaa cggcttttac gtccatcaaa
    47881 tataacactt gaagagattt ttttatacgg aggtaagctg caaactgagc agccaaccaa
    47941 agaagatgca gctcctgcag taaataaaaa gtatgcgaaa actttttctg gaaaggagca
    48001 gaacccagaa tataacaaag aaaaaataag aaaaaaagtt aaaaataaat caaaaaaaga
    48061 gagaaataaa ggagcaaggc gcaaataagc gcctttttat gctatgatta tgaaaacggg
    48121 aacaaaattg gttgccagag gaggggttat gtgaaagaac gtcaagaaga gatcttgcag
    48181 ctattgacag aaaaccaaca aggattgaca gcaagtgatg tagctgagcg cttaactatc
    48241 gatcgaagca atgcaagccg atatttaagt gaactataca aggcccatca tattgtaaaa
    48301 accgctggcc gaccagtcgt ttactctttg ccaacggaaa agtcaaaatc tgatgaagtg
    48361 catgtcgact cttctacgca agtgactttt gaaacactcg ttggagaaaa tgattcttta
    48421 aaagtcagca tacagcaggc aaaagctgcg attctttatc caccgcgggg gttgcatacg
    48481 attatttttg gagaaactgg tacagggaag tcgatgtttg ctgaatgtat gtatcatttt
    48541 gcaatcgatt cagagatgct ttctgcagat gcgccatttg tttctttcaa ttgtgcggat
    48601 tatgctcaaa atccgcaact gctctttgga cacatctttg ggatcaaaaa aggggcttac
    48661 acaggagcag ctcaagatag tcccggtcta atagccaaag cagacggcgg tatattgttt
    48721 ttagatgaga tccaccgttt accgccggaa gggcaggaaa tgctgtttac tttcattgat
    48781 aaaggtattt accgtccgct tggagaaagt gcacaagtac atgaagcatc tgttcagatc
    48841 attggggcga cgactgaatc ttcagaaagt ttcttatcta cgttcaatcg acggattcct
    48901 atggcaatca ccttgccgaa tcttgcttca cgttcactgg atgagcgtta tgaaatcatc
    48961 tctcttttta tcaaacaaga agcgaatcgt ttgaatcaac gtatcgatat cgaaaaagaa
    49021 gcgattcttg ccttcatgct ttatgatgcg gaaggaaata tcggtcaagt gaaacgagac
    49081 ttgaaactag tctgtgcaaa atctttttta cattaccgta ctcatcatga gaaaaagcta
    49141 atcattcgaa aagaagaact ttcccttcaa gtacaaaaag gattactgaa aatcaaggaa
    49201 ataccggaac gtctcgaccg attcatggac agcaaaagtc agtatttgac atttgaacca
    49261 ggatttgctg atgttgtctg gtcacaagat ccagaacgaa acatgcaggt ctataacgat
    49321 attgaggaaa aagtcacttc tctttccgaa accggtgtgg agaacattga tctagagacc
    49381 ctcatttcaa aagatgtgga tgcttatttc cagacatatg tcaaagaatt gactaaaagt
    49441 tcgattccca aagatctttt gccagatgaa atctggcggt taaccaatca attgtatgat
    49501 gtggctgaaa aacggttaga ccgtatttat aatgaaaaag ctcgttttgc ttttgctttg
    49561 catctgcaaa gtacattgga tcgtgtgaga gaaggtcata tgatcgtcca cccagatttg
    49621 aataacgtac ggaaaaattt taagagggaa ttccaggtag caatcgatct ttcaacgatc
    49681 attgaagaag aagaacaagt tgagattcct tttgatgaaa tcggctttat tagtatgttt
    49741 ctttctatca atctaggaga aagtgagcct gttaaagaaa acaaagtaga tgttctcata
    49801 ttgatgcacg gcagacagac tgcttcaagc atgttggaga tggcacaaga actattagga
    49861 acaaaagtag ggaaagcttt taatatgcca ctggttatgg aagttcaaac aatgtatgaa
    49921 gaagtgttac aatatgtcaa aaactatcaa gagcatttga cgaatggcct actcttgtta
    49981 acagatatgg gatcattaaa caccttcagt gatttgattt atgaagagac tggcattcgg
    50041 acgaaagcta tcacgatgac aagtaccatg attgtgatag aagcgatacg gatggcaagt
    50101 gttggaagaa gcttagaaga tatttatcaa aatatccagc tttcctttga atcgatcgta
    50161 cgtgatcagt tcaaagcgga aaagtctgaa actttacagc gtaaaaaagc cgtaatcgtg
    50221 acatgcttta ctggtgaagg agtagctgcc aaattatatc agcgtattat gccagtgatt
    50281 aatcaaaata aggtagaact gatccagatg cagtttttag aacgtgaatc attcaaaaaa
    50341 cacatcgatg ccttattgga agaatatgag atcaaagcaa ttgcgggaac agttgatgtt
    50401 gattatcaaa atatcccatt tttttcagcc tatgacgttt ttgatgatga aaaactaaat
    50461 attttaaagc gtatagctga tgatgagatc ccgacagcta aaattgtcga ttcacttgct
    50521 ggatcattaa gcgatgtctt ttctattgat gaactgatcc gttcattgca aaagacagtt
    50581 cagcagatcc agaccgattt gtcgattatc gtggaacctg gagtagatac tggaatcgtt
    50641 atccatttag ccttcatgtt agacggattg atcaaacaag aaagccatcg gcaatttgag
    50701 aaactgtcta ttttctcaaa aatttatcgt ttggaaatgg atatcgttcg ggcaaacttg
    50761 atggcactag aacgaaaata tcaggtaaaa attccagaag atgatatcgc atttcttaca
    50821 caaatgtttc ttgaaaataa aataaaaaaa tgaacacact aatcatactg tttacacact
    50881 aaacatctgt gtgcgcacta taaaaaagag ctaaaaagca tgaaaacgct atttcagctc
    50941 ttttttttat tggcatgctt cttgcgttat ataaaagtgt aaaaaaataa aaggagtgtt
    51001 aaaaatggca aaaaaaacaa tcatgttagt ttgttcagca gggatgagta cgagtttatt
    51061 ggtcacaaaa atgcaaaaag cagcagaaga aaaagggatg gaagccgata tttttgcggt
    51121 ctctgcctca gatgcagata ataatttaga atctaagaat gtcgatgtat tattattagg
    51181 tcctcaagta cgtttcatga aagctcaatt tgaacaaaaa cttgcgccta aaggtattcc
    51241 attagatgtc atcaatatgc aagactatgg catgatgaac ggagaaaaag ttcttgcaca
    51301 agcagaaaat ctaatgaaat aaatcaagta tgcagttggg ggtataacaa cgtggaagat
    51361 caacaaaatc tagaagccat catggggtta atcatgtttg gcggtaatgc aaagagcgat
    51421 gcaatggaag cgattgcggc tgctaaaatc ggtgattttg agttagcgga taggaaaatc
    51481 gctgatgcag aagaatcact tatacaagcg catcatgcac aaacagacat gttgacacaa
    51541 gaagcacaag gaaatcatat gcaagtgaca ttattgactg ttcacagtca agatcactta
    51601 atgacatcta ttgcgtttac tgatttagca aaagaaatca tcgaacttta tcgtcgcgta
    51661 tacagcgaat aaatacaact tcttttttga acggaagaag aaaaattttt gaacggattt
    51721 tttattttgt tataaggaga actctcttta tataaaaaac ttataggagg aagtaaaaaa
    51781 tgaacggact atcaagttgg atggagaaat acatcctgcc ggttgccgca aaaatcggag
    51841 cgcaaaaaca tttagttgct ttgcgtgatg cttttatcgg catactgccc gcaacaatgg
    51901 cgggatcagt cgctgtaatg atcaatgcaa ttattcgtga tttaccgcaa cagtttaaca
    51961 gtacctatgc tgacagttta gcagcagaca aaggaatctt tgccgtattg aatgtcatca
    52021 tcggaatcaa tggttttgtc tggaacggta cactagcaat cgccggattg atctttgcct
    52081 tttcttgggg gtataatctt gcccgagctt atggagtaaa tgatctagct ggggggatcg
    52141 ttggtttagc taccttgatt caaggtattt cctttggtcc agcattatca gcagcattag
    52201 atgtgaatgt tccttcaaat gtcgcagatg caatcaatag ttctgagatt ggtgcaacga
    52261 ttgcagatgg cacactgtca gttggattat ggggttggct aaaactagat catttgaatg
    52321 gaaacgctta ttttacagtt atgatcatgg gagctatttc agtcattatt ttttgtaaat
    52381 tgatgcttgc caacattacg atcaaaatgc ctgattcagt accaccagca gtatctaaag
    52441 catttgcagc cattttacca gcgacgatcg ctttatacgt cattgctatt atcaattata
    52501 tcgtaggtcg tgtagctgat gggcaactga tcattgattt ggtgcaaaaa tatattgcag
    52561 aaccattcct aggattatct caagggttgg gagcagtatt gatcgtcaca gtatttgttc
    52621 aagtattctg gttctttggt attcatggat caaatgtatt ggcaccagtg ctagagggaa
    52681 tctggggaca agcacaacta atcaatattg atattttcca aaaaggttat aacggaaaaa
    52741 ctggtacacc agccgtgtta gatgcaatcg aagatggaaa agcttatatg tgggtccgcg
    52801 gatcgttcga tgcttttgca tggtttggcg gatcaggcgg aacgatcgtc ttactgatag
    52861 ctatcttgat tttctctaaa cgtgcagatt atctaacagt agctaaatta tccattgggc
    52921 ctggtatctt caatatcaac gaaccagtga tgtttggtct gccaatcgta ttgaatgcaa
    52981 tcatgtttat cccattcttg attgctccag tagtagcaac agcaattgga tggacagcta
    53041 cttatcttgg cctagtcgct ccggtatcgc agcaagtcac atgggtagta ccgccgttct
    53101 tattgtcatt cttagcaaca ggagcggatt ggcgggcacc gatcgtaaca ttagtatgta
    53161 tggtcgtgac tttcttgatt tgggcaccat ttgtcatctc tgcaaacaaa atggaccctg
    53221 ccgcaggcga acaacaataa gatgaacgtg aggagcggga cgctttaagt ttcgttcctc
    53281 ataatgaatt ttcgaggagg aactgaaatg attacaattg ccagtgcaat gcaacaagat
    53341 gaaatcaaag aattgttaga aggatacaat gaagaaggaa tgacttttac atttaaagaa
    53401 aaacaaggaa tcaagctgtt ctttgaaaca agtgcagaag atccggaagt tgcggctaaa
    53461 aaagcaaagg aactaatcaa agcacagcct tggggaacag tcctttattt tcaatcaaca
    53521 gctgtttaga aaatggttta aaaagatagg atggttgaga tgataaaaaa cggtttattt
    53581 ttatgtagta ttggttcttt gatcgtgttg tatagtttga atccacattc gcaaaggtat
    53641 gatttgtata gtatggtcac aggtcttttt ctaacaggat taggaatcta tttcttcatg
    53701 aaggggaaaa aaagagaaga agcaaaggag aaaaataact aatggaaatc acgaaaaaat
    53761 taaatattcc agcatcgttt ttttacgata aagttatgga ctctgtcctt ttcgatgtac
    53821 gtaaagcgac cggcaaatca gtaactagaa aacagctgaa aaatttggag tacgtcaaac
    53881 aattctcgca aaataaccgg gctcgcataa aaatcgaaga agtcattgaa aaccagtctt
    53941 ataagtttcg tacatcgaca acgaagaatg aattcgtcgt tcattatcaa atcaaaccat
    54001 tggacgattc gacctgtgag gttcattaca cagaaaaaat ggagtcttat ggcatgcttc
    54061 aaaagttgaa tgatatgctt ttaggaacaa tcttgatgta ttttaaacgg agacgtttca
    54121 aaaaaatgct agatatgatc gaagcaacgt attaaatcaa tgaaacatgg tactatttag
    54181 ttattgaaaa atgaagtaag ctagaaggaa tttttcatag gaagatagat ctttcctgcg
    54241 aatcctcact agcttttttg taggaagggg cagcaaaatg acaaacaaag taagagagct
    54301 aggtgtatct tcgattgagg aaatgtttga tttagccact tatgcattta atggggcaga
    54361 tacacctgaa aggcgagaac gttttcgtac acttgcggag aattcttgga attacggatt
    54421 ttttgatgag gaggatcggc taacaagtca agtcatggcg accccttttc cagtgaattt
    54481 ttacggacaa gagtacctaa tggccggcat tggctatgtt gcttcttatc cagaagcaag
    54541 gggacaaggc gggatcaatc agatcatgga aaagatcctt gaagattgcc gtgatcgtaa
    54601 agtaagctta tcttatctgg cgccattctc ctatcccttc tatcggcgct atggctatga
    54661 acaatcattt gataaaatca gttatcaact aaacagtcgt gatgtgccgt ttatcaaaaa
    54721 aaaatctgga aaaataaagc gtatggattt tgaagatgca aaagctgcaa tcagaagaat
    54781 ttatgaacag atgccggaaa accaacgagc tggtctgaag cgcgaagact ggtggtatac
    54841 gtataaattc aagcaaaaag gcaattacca gtttgctttg cattttgatg ataatgagga
    54901 agcaacggga tatatcgttt atcagcttgc aacgtcttcc tttattattg cggagtgggg
    54961 atttttggat catgatgctt tttgcagcct tgttcgcttc gtaagttctc acaatggtgc
    55021 ttttgaaaca tttgaatatt catgcggcta cggcggtaat gatcaaaatt atctattaga
    55081 aaatccattt gcttctgtaa tgatcacacc ttatatgatg gcgaggatcg tagatatttc
    55141 tttgtttttg gaaaattatc catttaaaaa gagacaagct gcatttgccc tacaaataga
    55201 agaagatcat tatgcgaaat ggaatgaagg aatcgtggaa gtaacaatcg aaaatggaaa
    55261 aaatcatctc tcaaaagtag aacaaacaaa actcccaatc gttcgaggaa gtatccaaca
    55321 tatcacgcag ctattattag ggtatcgtaa agtggaagag ttggtattcc aagaaagaat
    55381 acaggtgaca aaagaatatg aagaaacgtt aagctcaatc ttgccacagc aacagccaat
    55441 tcttaatgac tatttttgat aaaaaacggg tgatgacaat atttttgtca ccaccctttt
    55501 gtttttcatt tggcatttga aggaaaatgt taaagtaaat cctctttttt cgtaaatttg
    55561 aattttttgt tttcttttgt tttagggttt tcttttttgt ggtcttggat tgcttctttc
    55621 gtaatctttt cccgattttt tttagatgaa tctaagcgtt ctgcgtatac tttttctttt
    55681 ggcttatcat tcattggaaa ggacctccct acattatttc attctacctc tagtttatca
    55741 tctgttaagg actagtggaa acgttttgcc taacttataa gctgattttt ttagcagata
    55801 agcttgcaag taaaaaacaa acaagctaca attgatttat tgaaattgtt cctgtagctc
    55861 agtaggatag agcaatcgcc tcctaagcga taggtcgctg gttcaattcc agtcaggaac
    55921 gtataaataa cccctgtatc ctttgcggta caggggtttg ttttgtattt gtgccaaaaa
    55981 tgtgccaaac tagttatcat ttttatataa actatcgaac acagtattga ttttttcttt
    56041 atcctgatct tcaagctctc gaacaatatg aagataagta gacattgtag tttctaaact
    56101 gctatgtcct aaacgtttcg atacgctaag tatatttact ccttgataaa gtaaaataga
    56161 tgcatgtgta tgccttagtc catgcaaagt aaattgcttt tcaatcccta attcttttaa
    56221 ttttttgcgc aaatatttgc tgacggcgtt tgatgaaact aatccatttt ttaagttgaa
    56281 aaaaacaaaa ttatgagggt ttttgatttc aaaattctcg aataattctt tttgattgat
    56341 tttaaacttt tttaagagat caatcgtatg tttatcaatg gaaattttcc tatttgaagt
    56401 ttcgtttttt gtttttcccc attcatttaa tttataattc catgttttat taatagtgat
    56461 gatttgatct tcgaaatcga tatcttccca tgttaatcct agaagttcag caaatcgcat
    56521 tccagtggca ccagcaacta acacgagcat aggagaagag tactgcgcag aaaggttttc
    56581 ttctacgagt ttcattaaag atttaaattg atcataatct aaatatttat cttcttttgg
    56641 cttcaatgaa tcttttcctt tgattactgc ttttctagtt ggatcaaacg gaattaatcc
    56701 ttcttctacg gcatctttca acgacgctct aatatggtta ttaaacttaa taacggtaga
    56761 ttttacatgg tctttagcat acttatttaa aaagcgttga tatcccactc tatctaaatc
    56821 agaaatcaat accgctggca tatatttctt tatgttcatt aacgtatctt cgtattttct
    56881 ataagtgata ggagatactg ttccttcttt ataaagttgc atccaatcct caaagtaatc
    56941 tgataataat agatttttgc gatccccttt aaggcctttg ttcaagttat attctaattc
    57001 gttggcggca tcttttgctt ctccttttgt cttaaatcct gattttctta tcttcgtgta
    57061 ttttccgtca tcttttttgt atgaaatttc gtattgccaa ctatttccac gtttgactaa
    57121 tcttgccata attgaatcag ctctctttct ctgatacaat aggaactata aagaagccca
    57181 ttgtttgggt tttattattt tttgcacatc ctttttgctt gccggcgcaa ggatgtgcta
    57241 tttgtcgttt taatggtata attggatgtg tattagatca tccaatcaga ggtgtttatt
    57301 atgattaaag tgatttttaa aagaataact gaacttccag agtttagatt atttgttgat
    57361 tattggtatg tctggttttt tattgttgca ttgctgatag gtctagatgt catattagaa
    57421 agaaaactta agtaagagtt gagttaataa attgaattcc taaatgagat attttgtagc
    57481 ccatattttg tttgccgaca tcacctaata ctatggagct aatcgttttt tctaacagac
    57541 caaaatcaga taatctattt aagctaccat ctattagctc ttgtgaatag ccagacatgc
    57601 tgatatttcc agtgaaattt tcgtttccac gactcattaa gcatgctaac aattgaatct
    57661 caatcgatgt taattgattc agtacaagaa gataatactc ctcgttatcc catgaagcgt
    57721 tgttaattcg aagtaaacaa ttcttataaa gatttttata catatctatt ttattttgtg
    57781 cctttgattt ttctacctcg tcatttatcc cttcgaatat tccaatgagt tggtccttgt
    57841 tctctaagtt gaaaacatct tctggaattt gatcgcgaat gttttctaat gattcgttta
    57901 aatttttgta gaatttctcg atgcgtttga aacgcttctc gtttttagac ccaaaataaa
    57961 gtgactgcac aacgccacca atataaggaa ttgctccaat accgccttca actattaatt
    58021 caagatattc ttttttggtt agtttttcat tgctattatc catagttacc tcctattttg
    58081 tcatttcctt tacaagcatt gtgactcctg atggcactct tttgtatagg ttttattatt
    58141 gtgcacaatc taactcttag tggggcgtgg ttcatttatt ttttacttac tttactatta
    58201 atcatatccc attgagcata ggatttttct atgttcgatc ctcttgaact aatttctcct
    58261 gtaaaagtta ctttatcgcc ctttgtaaag gtatcagcat tgtttaagtc cttcgcaaaa
    58321 attacatatg aaactttagg tgttgcagaa atatcattcc aacttttatc atcgtactct
    58381 ttgtctgcta taatggctat tctagtagtc attgattcga taacagttcc ttcaaaagta
    58441 tatgttttac cactaatata tttagtaaaa gctgtatttt gctcagtagc tggagtaata
    58501 gaataataac tatctaagaa agcaccccag tcatgattat ttgtctctaa aaattcttca
    58561 aattgagtta ggcttttatt gctgctgtta gatgtagtag tttgagtatc agatgatgag
    58621 ctttcctgtt tgttattgtc atttgcctcg tttgatgagg aaagcgagaa acctacggta
    58681 gtaaaaataa tcccgataat aaggagaatc ataggtgttt tcttatttgt ttttttgata
    58741 aatcttacaa ttaacatgat taaacctata ataaataaaa caataccagt aaaaccaaat
    58801 ataccgtcca ttaatagatc ctctttctct tttgatattg aatattataa accaagcaat
    58861 tcttttttct taaaatcgaa ttcttcttga ttaataatcc cttcatctaa caactcttta
    58921 tactttttaa tttcatctgc acttgaaatt tgtgtttctt tggaattatt atctaatata
    58981 gattgttgct tctctatata tttttttaaa ttttccattt cattccaata tttttttgta
    59041 aacataacgg tgttttcatc tttcgtggcc gctaatatac ctccgcgact ttcgtttccg
    59101 cctagcaagg taaattgtat ataaccatta ctcatacctg gtttttttaa ttgtacggct
    59161 gatatatttt taaaggggat agttttttct cctttaatac cttgattaac taaatttata
    59221 aatccttttc taataattga aatagaatca tcagttactt taatcaaggt ttttcctgga
    59281 gattttatta aaatctcttt ttccattttt tcttcctcat ttctgtgata tgattttttt
    59341 tgtagaagct catatatgag gaaagagccc gtgttgcagc acgggctttt ttaatgtttt
    59401 gctgaaatcg gtttttttaa taattcttga tgtattcata catattgcct tgcgtaagaa
    59461 tattttttct taaaatagca ttggcagaca gcataacaag tgtatctgag cttattctga
    59521 atagaataat actccataaa tttttcgaga ttaaactgag attcatcagt cagttcgttc
    59581 tcaatataga tatttaataa aattagaata gctattttat ctgcttctgt ttcaaatttt
    59641 gagtgaaaag ttgtagaagt atcgtacaaa actgaaaatt caaaatgaga ggcgctgaaa
    59701 tgtgcaagtt catgagataa atgaaaggct tctgcagttt caccgtatag attttcattc
    59761 aaaaaaatga ttctgggttt tggatagtag aaaccaggct ctttcatctc catataaact
    59821 actttcaaat tgtattcgct caacatttct ttcaatttca aatacataca aaccatcact
    59881 ccaactattc attttcctct aaagctttag caattgcaat cgctttacgc attgtctcct
    59941 tagatatttc ttttccatca aaagaaaaaa cagtatcgtc ttctgataaa tccacatgtt
    60001 taggggtttc tctttcttct ctacctagaa ggtagtctac agagacatcg aaatagtcag
    60061 caatccgact taattcatct gaattaggag tattattttt ccactttgct agataaccgt
    60121 tagaatatcc aaaattaatt tctaactgcc gtatagatat ctttttcctc ttcgccaagt
    60181 cttttattat ttcataggta ttcatcgata aaacaacctt tctaaatgtt tacaaaaaaa
    60241 gtttagaaaa atacgcagaa ataatttgac taattctgag taatgagcta tactatatct
    60301 tgtaaacaga tttaacaact aaaaagacaa caaaaaacac tattgatatg taaatgcaga
    60361 ccgccaagaa agctttaaaa tcaatgtttt tatgtcttat ttaattatgc tcttagtata
    60421 gagtattact cagatactgt caattgaatt tagaaaaaag ttgttaaatt tgtttacgaa
    60481 tataaaagaa aggaatgaga aatatgaaca caccgcaaat ttttaatttc gaacaaaacg
    60541 aagttcggac aattttagta aatgatgaac catattttgt aggcaaagac gttgcgagtg
    60601 ttttgggtta ttcaaacact aaagatgctt tgtcgcggca tgtagatttg gaagataaga
    60661 tggggtcgcg aatcacgacc tcaggtcaat caagagagat gacaatcatc aacgaatctg
    60721 gtttgtacag tttaatctta aaatcaaaac ttccctctgc caaaaaattt aaacgttggg
    60781 taacaagtga agtgttgcca gcaattagaa aacatggagg ttatctaact ccagaaaaag
    60841 tagaagaagc tttgcttaat ccagatacaa tcattcaatt agcaactcaa ctaaaagaag
    60901 aaagaactgg aagattaatc gcagaacaaa agattgcaga gtacgaacct aaaatctcct
    60961 atttagatag catattatct tctacagatt cagtaacaat tagtcagatt gcagcagatt
    61021 atgggatgtc tccacaacag atgaataaat tacttcataa actaggtatt cagaaaaaag
    61081 tcggtaacca atggttattg tgcaaaaaac acatgaacca aggatacaca aaatctcata
    61141 caactgagat cccgaaagcc gatggtggca ctaaaattgt aatgaatacc aaatggacac
    61201 agaaagggcg tctatttatc tacgaattac taaaaaaaga aggatattac cctcaaatgg
    61261 atttagagga aattggttag aaaggagttt tagtatgact gacattgcag aaatcactca
    61321 acgagataga gaaaaaatca aagaatatgt cgaaagttca aagttcttaa cttacaccat
    61381 gcttgctgaa agatttggaa ttagcaaaag ctacttatct ttaattttaa acggtaaaaa
    61441 gacttctgca gaagcaaaca gaattataga ttcgattatc actatgtacg aattgtaaag
    61501 ggaggaacag ctaatgcaat atctagaagc aaaaatccca attccagaag gctatgtaat
    61561 tatctcccaa gtggattatg aggagttaaa aaaagctgat gatactggta gatggatgac
    61621 gttgccagaa gtgctagaac ggattaacag aaaatatgat tggtttactt ctagagtttt
    61681 aaagaaccca agatatagaa atattatcga tatcgagaaa aacaaaaatg gatttgtcta
    61741 ttatccagtt gaaggaagag acacatatct atttttaaga agtaaaacac ttgaattttt
    61801 agaaacaaat ttttcggaaa tcttaaggag gcaagcggat gggaaaattt aatagagcgc
    61861 tagtgttcag cgcaccgcta atcatctacg ctttaggact ttggggaagc aggcaagcgt
    61921 tgataggaac gattgtttac atggtttgga tttttatggg gcttgatgaa gctgaagctg
    61981 agtacagagc gaaaaagcca accgaggggg ctgactaaaa atgaatagaa aagagaaact
    62041 agaatgtata ttactattac tcagtttaat tctttcacta atttctctat tgggaagttt
    62101 gtatttttga tatcaacaat tttgtggatt tgggttctat ttagagtaaa tatcattttt
    62161 gtttggtgat taaggagttt attaaaatta attttcccta cttcaaatgc aaggtaacaa
    62221 gatatagcac ttttaggagg aatatttagc ggtacagctt cagagtatat tctattatca
    62281 tcgactgatc ctcctttagc aattaatttc ttaaatggag tagctgacca tctgcggttt
    62341 aagttagcgg atgaaagttc taaattaact aacatagctg gttctgttga atagttagaa
    62401 atgattactt ttgtataaat catatctgca gcgaagtaag aagcattaag ttcaacttct
    62461 agttgagggc gcattccttt tatttttatc cctgttaaaa acgtactcaa tataaaactt
    62521 attattgaca tccattgaaa aattgttaag tgaaaaaaat tcaaaataaa caccaccagt
    62581 ttttaactaa attataccaa aaaggagaga agaaataatg caagaattag taattttgaa
    62641 aaataaagaa gctgtgacta cgagcttaca agtggcagaa agcttcgaga aaaaacatca
    62701 acatgtttta agagatattg atgcactaaa aaaagatgtg tccaattttg gacagatgtt
    62761 tgtagaaggt aatgaaccag attcatatgg cagaaatcga cgagttttct tcattagtag
    62821 agatggattt ttcttgctgg ctatggggtt tacaggaaag aaagctatct acttcaaaca
    62881 aaaatacatt gaagcattca acgaaatgga agatgttatt cgcaagaata ctgttcctca
    62941 aacaattgaa gatatgatga tctatcaatt agaagaaatg aaagatgtta aaaaagatgt
    63001 ttccatgctt aaagatacta tgcgaattag cggacaacaa gagtttgaaa ttaagcaaaa
    63061 aggaaatatg aaagttatgg gagttctagg gggaaaagaa agccgagctt atgaagaaat
    63121 cagcaaaaaa gtattctcaa aattttggtc tgaatttaaa cgtacctttt caatcccaag
    63181 atatggcgag ttacctcgta agagattcga tgatgctgtt tcatttattg aaatgtggtt
    63241 gccagaaact gcgatccgta tggaaattga tcaactgaac agacaacaga gactttttgg
    63301 tgatgacaat gaatagagct gaagcgctaa gaatagggac ggcaattgct aattgctggt
    63361 ggaaatacta taaaccaatc atcctaagcc aacaacatat tgacaagcaa aaagcatggc
    63421 aacaaataaa aaagtgactc cgccggcaag caaagagtca caaagaaaac acatcataag
    63481 gagattttag catatggaaa aagaactttc cactctagat caatatttga ttgatcctga
    63541 ttggggcaag ccgaaaattg aggaaacaag tggtcgaaaa atcagacgaa atcttttgac
    63601 gaatgaagaa ctagcttggg atcaagatga tttaggcaac catgtaacta tttgggatca
    63661 tgtttatctt atccatctat cgaagcattc gaataaacct gaatatattt acgtcatcga
    63721 agatggcttg attgatgcgc tagaagagta cgacagagat aacttgattg atatctctta
    63781 ttacggacca ggtaagaaat acattgctga aatggaggca gaatttgatg agtgaaatca
    63841 aagggacaac gaactttgaa aaacttttta gtcgtaagtt aaataaaatt ctcaagaaaa
    63901 aaggaaattt tgattattta tcttgggctc acgcgtggga gattatgaaa aagaatgatc
    63961 cacaggcaac ggtaactatt aatgagtaca aacactacag ggttgtttct ggaactcatc
    64021 aagactttct tgttgaggaa tataaacctt ttcttatgga tgaaactggg acttatgtat
    64081 ctgtctcagt aacggttaaa ggacacacgg aaaccgaatt atttcctgtt ttagattatc
    64141 gaaaccaacc agttgttaaa ccaaatgcaa tgcaaatcaa taactcattg aagcgatgct
    64201 ttgtgaaagc attggctcta cacggactgg gattatatgt atttcaaggg gaagatattc
    64261 caacaccacc tagaatcgat acaaagaaat taaacatgct agagacgatt ctagaagctt
    64321 tcaatgagca gatgggtaaa gatatgacca aaaccttaat cgaatatgtt aatgagcaga
    64381 cagataaatt agggctctta gctgataacg ttgaaactat tgaacagtta agctatgagc
    64441 aatgtgcctt gatggagcga gcaatagcag ctaagagaaa agaattagat aagaagtgat
    64501 atgagtgttt aaaccattaa tcgattcata ttcagcggtt ctgaaaaagt tcaaaggaaa
    64561 agacataagc gcaaccatca atgaggaagt gaacattgat cgactaaaga cgatgtatga
    64621 cggctacgat ggtgatcgag tcattgaaat tcgttttatt gatcctagac gtttcaccgt
    64681 acagcaacga aacttcatct atgcgctgat aggcgatatt tttatcgata caggcatgcc
    64741 aacggacttc tggaaggaat tcttctactt tcgttttgaa ggtgtcacag ggcgcaaaat
    64801 aagcctgaaa gacgaatcga atacaactgt gagtgatgcc aatgtcttag caaatatcat
    64861 cttagatttc atctttgaac atcatattcc tttcaaagaa ggctatgaga ttttacctgc
    64921 gaatcaagaa tattacttct acaaatgcat cacaaaaaga gtctgctgca tctgtggcaa
    64981 aacaggagct gacatcgatc actttgacaa agcgctagga agacgaaagc gcaaagaagt
    65041 tgatcatgca gagtacacat ttgcagcact ctgcagaatc catcacacag agaagcacaa
    65101 aataggtgtg attaatttca aaaataaata tcaaatcaaa gggatcaaat taaaccagaa
    65161 aacaatcaaa aagttaaata ttggagggga aaaatgacag aacatcgaag ttattatgcg
    65221 attataccag ccaacgtaag gtacgacaaa agacttaaac caaatactaa gttgttatac
    65281 ggagagataa cggccttgtg taatgaaaga ggcttttgtt gggcaggcaa tgagtacttt
    65341 gcagatttat atggtgtgaa taaagagacc atatcgcgat gggtaagtga tttgattaag
    65401 tttggatact tgaatcggga aatcatttac aaagagggta ccaatcaaat aatcaatagg
    65461 tacctacgaa ttaatcaata ccctattgac gaaaaacgca atacccctat tgacgaaaaa
    65521 gtcaaagata ataatacatc tattaataat acatttaata atacaaaaga atatataaga
    65581 gagttaccgc cttcgaaaaa atcgaaggct aagcccgtcc gtcataaata cggagagtat
    65641 aaaaatgttc ttttgtcaga tgagcagatg gagaaactca aaacagaatt ccctaatgat
    65701 taccaagagc gaatcgaacg actgtcagag tattgtgaat catctggtaa gacttataaa
    65761 aactatttgg caactattcg aagttgggca aggaaagaaa aaagtgaacc taagaacgca
    65821 agcagtggat acaagcgcac aggaagacga gagaagcttc ctgaatgggc aatcgaccaa
    65881 gaagcctatc tcaagaaaaa agcgctagaa cgagctaata gacaatcaaa agcaccattc
    65941 taagaggtgg aaaaatgaag atcgattatc tagaactaat taatgaaata gcgaattata
    66001 aaaagggcga ggaattagac gtcctgagag acgtatatga tcaactcgaa gaagctggaa
    66061 ttgaaggaat taagaatgat cgttcgagtt ggagtaaact cagatactat ttcgcactct
    66121 atatcgatgg aacgcaatta agaaatttag catataccaa attactattc attgattgtg
    66181 ttaaaggctt gcaaaaacat cttaatgaac ttgagcaggt gtaaacaaga tggacctaaa
    66241 gacatttaca gcacagatcg aattaatgca tcaagaagct ttaagacaaa gtgtgtcgta
    66301 cgaagacaag tggctcaaca catttcatgg cggacgtgag agcgcacttg atcaagtact
    66361 caaattactg aaaggagaat gtcgggatgg ataagaaagc ggcaatgcag cgaattatcg
    66421 aattgactta ttcagaagat tggcaggaag ataaaaaaat agttgcagaa gtccaaaagc
    66481 tcggtaaatc aatgtggact gaaaagccca aacggagaac gccgagaaag attgcaatct
    66541 ggcatggtga tcgaattcta gtaacgggta ccgctaaaca gttatctgaa attactggtc
    66601 tgagcaaaaa tattatttgg gatagagcta ggagcttatg gattgattca aaaggaagac
    66661 agtttaggta tgtggaggag aaataatgga cgaactaatc gcgaaagtag agcagtgggc
    66721 aaaagataag ggattggatc aagcggattt cagcaagcaa atgttaaaaa cggtcgaaga
    66781 aatcggggaa gttgccgctt ctctagctag aaaagatgaa catggtttaa gagacggaat
    66841 tggcgatgta gtagtaacct tgatcatctt ggcaatgcaa aatgatatgg atttatacga
    66901 gtgcttggat tttgcttatg acgaaatcaa aggtcggact ggaaagatgg tcaatggtgt
    66961 tttcgttaag tccagtgacc tgaaataatt ccattaatgg aagaaataac tgaataggag
    67021 gatagacatg attgatgcaa aacaagtgca aaaacagaaa gatggaatgt tgatgtttga
    67081 agcatatgtt ctaccatttc ttaatcaatt tgaagttttg gaatgctctg ccagcggaga
    67141 agaattggag tatgttgtaa ttcgtgaaac caaagaaaac gttcagaagc taaacgaatt
    67201 tctttgcact attaactgtt gggatatgat agctccagga tttttatgtc cagctatggg
    67261 agaatttttg gaatattgtc gtcttgaaga tgcaggcact ttggatttag cttatctggt
    67321 atacaactac ctaaacatca acacagatca tttgtggttt ggcactgccg aaagaaaatg
    67381 ggtagtccgc taaccaccaa tatcaatgaa aaaaggcggt ttcctgaaag tggaattgcc
    67441 gccattgctg taaggttctt tacgcgactc aaggtcacag aaatataaat gcaaatctct
    67501 tttgaagtga tccaaagctc tcggaaaatg gaagtttttt gtttcaaaga ataaatgaga
    67561 tttgcgtcaa gaacctgaga gttatttcca aaaaagcttc caaagaaccg aatgagctaa
    67621 ggacatcatg tgtaaacaca taatatagct ctcctttcac ggctaatcat aacatgaaaa
    67681 atattcgatg tcagtaggaa aaaaggtgaa ttatgaaaag aatattaaat tatcccggca
    67741 gtaacagatt ctgcaattaa actccgaagc tgatgtatca gtatcaactg tagaatatta
    67801 tgagcgtgga gaaagtcaag cagtcacaaa tgtaaaaatc gataacgtca acggtcttat
    67861 ctgtttagat gaaggcgata gtatgaatga aattgctgca gaccatgaat tatcagaaac
    67921 tgctttaatt gatcaaatcg aattagctag acaatacttt gttccatatg cagatacctg
    67981 gtccaagcat aagaatgaca tcatttttcc agaggaacaa tgagagcaac tgatccagta
    68041 attatccttg aggaagccaa atttatttgg actcacgaag agatagagca agcacgcttg
    68101 ctcttttctc aaggagttaa gccgagcaaa gtagctgaaa taatgggtca aaagattctt
    68161 gatgtcggat tgcttttgct ccatctagca gaaaaaattt gatttgaggt gaaaatgatg
    68221 attcaacttg caggcatcca aacagggaaa atttattttt ctggagaaag caaaagcgaa
    68281 gccaaccaat ggctattgaa aacttacacg aacaataaga agctaaggaa aaaatatcct
    68341 gatttgttgc taaaggatga tcagattatg ccggaaccga tgattttgac ccgtaaggag
    68401 ggaagttgaa actaaaaaac cagccgtgtg ggctggagtt gctatttctt tttaataatg
    68461 agtttttcta aatttaattc attctcacga gtttgcgtcc aataatcaga ataatatttt
    68521 aattgtttaa gagtaggaat gtcgtcttcg gtaaaattag aaaataattc tggagaatta
    68581 tcttttttta attgttctaa tcctgattct ttaaaacgtt gttcaacgag ttttactaaa
    68641 ttgttgatat tcatgataaa tcaccttttt aaatttaatt tcgatttgat taatcgatac
    68701 aagtattata tcaaataaaa aagctgattt agggggtctt acaaaaacac taaaattttt
    68761 tcaaaaagag gaggaacggt ggtgaaagtc agagaacaat tcaagatagt gagcgacgaa
    68821 ggtaatgttt acaaaataaa gatcgttaat attaatgaat atcgagaagc agatcacaaa
    68881 tatttggttg acttatacat gaacgataaa tatttgggag aaggattttg tggagattca
    68941 ctttttgatg atgagcgaat tttgaaggag gaaacagaat gatccacgaa ctaaaaatac
    69001 taccagaata ttttgaagca gtgacgagcg gacgcaaaca attcgagatc cgcaagaatg
    69061 atcgtgactt caaagttggt gatcagttaa tactacgtga atataaaaaa tacattaaac
    69121 atgaattacc tggactaacg agaccaacat ttagttacac tggtgactca tacaaagcag
    69181 agatcactta tattactgat tacatgcaga agtccggata tgtagtgttg gggattaaag
    69241 gggtgaatta aatgaaagta tacatcttag ctatcacaga gggcacatgg atgttcccag
    69301 tcggttccgg aaaaatctat aaatcaaaaa cagctgctta taaagcattt gaaaaataca
    69361 aaaaagaaaa cggtggcggc actaacgcaa agattctagt ggctgataac tggcatgaag
    69421 aaggtgaacg taattgattt acctgttcat tttcgtggta ctaactattt ttatgctagt
    69481 aatcttattc aaaaaaagat gagtaaagac gaacgaaaaa ttctggccaa cttttattta
    69541 gtattaaaaa tcgaacaaaa tgtgttttcg gaaaggagaa ttgtatggga aaaacaaaat
    69601 caaagattaa gaagaaaaaa cgacgtttgg aacaaaaggc aatccagaat ggaacggcta
    69661 agaaaaaata aaaaagctac ccctttcgag atagcagcgc aatactattc taccataagg
    69721 ggtggcgttt gtgagatttc attggttaaa agattaccaa gaactagatg agcagattct
    69781 ttacttaaag tggaatctta ataaaagtaa gcttgaattg aatcgatggg tctgtggtga
    69841 tttagcaaac gtccgcatcg aaaagaattc gagaacttct tctttagaag agaatatcca
    69901 aaagatagaa aatgaattgg aattgttgat cgagcaaaaa gaagaaatgt tattgctgat
    69961 agatagcttt tctggtatcg ataatcaaat tgttaagatg aaatatgttg atcaaatgag
    70021 cttagaagat attgctgaat ctgttgggta tagctcatcg tatgtcagac agcgacatgc
    70081 agaaattaga aagacattga actttttaga tgagtatgaa cacagacaag ctgaccgatt
    70141 gaaaaaagaa aacgaaattg atttttacaa tagcgagaag tacaaagaac aactctcttt
    70201 attctaaaat tacactgttc actaaatgtt cgtaaacatt gttagcagca tgatattcta
    70261 ttagtgtcaa aaaaatatga aagagccaag atatcccaac cgttttatta attggtatct
    70321 gtggctcttt tattctttta attagtagtt ggcataaagt tatttccatt tataaattga
    70381 tcagctaaat cagcatcata atcagccgcg ataaattgcg ctgctgattt aataaatgca
    70441 tccatatcat gaacatcttt attagtatgt ctacgaacat agtgcgtttc atcattacca
    70501 atccaagtag cagctttggc taatctttgg agtttaggaa aatcattcaa ataattttca
    70561 attgttttac ctaataacat tttttttatt ttatcttcat cctctgaatt tttagatatt
    70621 gcataatctt taattagaaa ttctagagct tttctataac caactccagc aatttgttcc
    70681 aattgctcag atttagcttg tgtggcttga gtataaatat caacaaatgt aggagaaact
    70741 ttttcaatat tttctggtaa atctatttta atcggcggac ggtaataatc tttaattaaa
    70801 atattttcag aatatccttc atcttcatta aacgtattta taaattgtaa actataaaat
    70861 ttgttgcact catcatttat acactgtact aatacgccaa agattctatc attatcgaac
    70921 gcactatatg atgaaacccc aaaatacaca tacggagtca ttatatttcc gcaatgcgga
    70981 caactatttg gcaaataaat tgtctctgta ttttgacgat cacgatactt aatattaact
    71041 tcttgaagca ttaaaacacc tcgcatcttt tttataaatc ataccacaaa atatctgata
    71101 ggagagtaaa caaatgaatg attttcatga agcggtactt agtattgaag tagaatcaag
    71161 tttggctaaa gtttataaga aagcaattga agcagagaat agtccgtaca gagaaaattg
    71221 gaatggtaat catgcacatg tgcaagtgga gaatgatgat tacagaactg gaatgaatac
    71281 gttagttatt tctttattgt cacacactct acccaatctg caggaaacga ttgaatggta
    71341 tgagcgaatg ggcgcaaaag taatccgtac aaattacaaa ggagagaatt aaaatggcaa
    71401 tgatcaaaat tagaacatcc attagtggaa cagagtattg ggattcagaa aagaaaagga
    71461 cagtagttgt tccgaaagat caagaaccaa attttgaagt aaaagaagaa aaaggaattt
    71521 tgagtgatgg caaatcattt gttgctgtca atggcgaact catagctcat agcagtgaaa
    71581 ttcttgatag cgatggaaac actgctgcta attttgatgg agacgaagct actaatgatc
    71641 agtccactga agatacggat gaactggaca atatgactgc aaaagaattg cgtgcatatg
    71701 ctaagaaaca tggtattgat attcctggtg ctatccgtgc aaaaggcgat atcataaatc
    71761 ttattcgtga agtagaatga aatactgtca gtttgacgga tgcacgaaca aaatagcaaa
    71821 gggtatctac tgtactgaac acaagagatc aagcagatca cgcaagaaga agcagcaagc
    71881 aaagtctgtt tatcatcatg agaacaaacc attctatcga acgcaagcat ggaaagatat
    71941 gcgtcaattt atttatgaaa gagaaggtgg tcattgtcag cggtgtggtc agttcatatt
    72001 tggaaagaga gcacacgtcc atcacattgt accaatcaaa gacaacgaac tgcttaagct
    72061 tgatccaaac aatctcatgc tattatgttc aaaatgtcat ccaattgttg aaaacgaaac
    72121 ggaagacaaa aaagtttttc cttcgtattt caattgaagc ccccctattc attttcaaaa
    72181 ttttttcgcg tggggagata gggtagcggg gagtcacgcg catcgttagg tcaaattttt
    72241 caaaaaacaa aggggggtgt atacaaaaat gacgactaaa gcgcaacgta aagcgattat
    72301 tgatgaaaaa gtaagtgctg aaaaagctcg tatcttagaa ataatgaatt tgtctgattt
    72361 gtacaccatc actcttgatc cattaatcga atcatacttg gatatttttg aaatttacca
    72421 acacaaatat ttattgtgga aggaaaaagg atttccggag acgcaaaaat tcacgaataa
    72481 atcaggagct actaatcaat caaaacatcc attggcacaa caagtagaaa cttgggcaga
    72541 taagaagatg aaagctctag atttattagg attaacaaac aaagcaaaaa ctggtagact
    72601 aattgctgga ggatcgaccg caagaaaaga tgaagaaatt acacgcccag aagtaaagcc
    72661 agtagatgaa ctagcagcgc acagaaataa atggcgtaag aaggcaggtg ctgaaaaatg
    72721 attgaacctg gtgtaaatta tgctgattta tttgcaaaag aagtaagaaa gaaacctaag
    72781 aagtatccta agaccgttcg tttagcaata gatcgctggt atcagtggaa aaaaagaaaa
    72841 gatatttggt ttgatgtaga tcgcgcgaat gaaatgatgg attgggtaga atcatttatt
    72901 gttcacacaa aaggagaaat ggtgggaaaa ccgtttcttt tagaaccctg ggaaaaattt
    72961 atttattctt ggatttatgg ttgggttaaa gaaaatgaaa aaggccaaat tgtacgcgtt
    73021 actcgtgagg cttatgtcca gattccaaaa aagaatggta aaacattgat tgctgtcggt
    73081 tcattaggat atgcaatgta cggagaaggg gcgttatcag ttgattgcta tgcttgcgca
    73141 tctgattttg ctcaagcgca atatgctgcc aaaccttttg cagttacaat cttaaacaat
    73201 ccaatcctac ttgaaggaac taaaatattt agggggccaa agggaactgt ttctagtatt
    73261 acttatgact atattcatga agatatggct tactcaaata aatttattgt tcaaaccaag
    73321 aatattgata atatcgaggg atccaatcca tattttgtat tgaacgatga attgcataaa
    73381 caggagaaaa tggaacagta tgacaatttt aagtctgctc agatatcttt accacagcca
    73441 ttgatgttta atatctcaac tgctgggaaa ggttcgtcgt ctgttggaat gcgtgtatat
    73501 cgcgaagcaa aagaagtgtt gaaacgtgat gataatgatt caaactttgt tctaatctat
    73561 gagccaaata aaaattatga ttggacggat agaaaagtct gggaaatgtg caatcctaac
    73621 tggggaatat cagtcgattt atctgcttta gaatcggcat ttaaaacagc gcaacggtca
    73681 gctcattcca aagctgaatt tctaacgaaa cacttggatg tatttgtgaa cggcgcggat
    73741 aatttctttg aacaggatca agtagagcca tgtttagtta ccacacaaga acttggtgat
    73801 ttaagtggcg aaccatgtta tatcggttta gatttatcac gtacacgaga tttgacctgt
    73861 gtatctttaa acttcccaac atgggatgaa gatggtaaag cggttcttaa agtaaagcaa
    73921 ctttatttta ttccgaatga agatttagag tttcgagaaa aagaagataa tgttccttac
    73981 agtgatttgg ctgaacaagg tttcgttgaa ttttgcgacg gaaaaatgat tgatcaagat
    74041 cagattttgc agtacataga ggactgcatg aatttatatg atattcagca agtgaattat
    74101 gatccagcga tgagtgacaa acttgttgag aaattagaaa acttaggatt ggaatgcgtt
    74161 gaagttgctc aatatcctaa agtattgaat gctccttttg acgatgtcga acggttgttt
    74221 tatgagaaac gaattcaatt tgataatcca ttattccttt attgcacttt gaacgttgta
    74281 gcaattacta acatcaatgg acaaaaagcg ccaagtaaac gtcaatcaaa gaaaaagatt
    74341 gatgggttcg tggcgttttt gtgtggtcat aaggaaacga tgaatcaaat gacagatatt
    74401 gattcagatg agttagatga ttacctaagt tccatctata gataaataga aaggcggtga
    74461 aaaaaattga aattacgtga tcggttatcg aatgcagtct attcttttat ggaaaaacgt
    74521 ggatatatcg aggatatttt tggacactac acacgttatg gtcaaagata tgtgacggat
    74581 tcatctatca tggaatcttc tgatgtttat gaattagttc aagacatatc taatcaggtg
    74641 gcattggcca caccaattgt tattggtccc gacggcaacg aagtcaaaga tcatcactta
    74701 ctaaatattt tgaagagtcc caatgattat ttgacaggat tcgaatttac caaattggaa
    74761 acaaatactt tattgatcaa tggtgagaca ttcccattaa cggataggga tcagcttcat
    74821 ttggcgtacg gtgtaacaac taaaatcaat gaacgacttc aagaagaatt tgaaatgaat
    74881 ggtcaaaaaa tacctggtca aatgattcga cacattaaga acatcggaac tgattcatta
    74941 aaaggtgctg gaataattga tcttgcaaga aacactctag aaggcgttct gagcgctgaa
    75001 aaagttttga cggacaaata tactaaaggc ggtttacttg cgttcatgct taaactagac
    75061 gcccacatca atccaaataa tagcgcccaa acaaaaattg tcaaagctat attggatcaa
    75121 ctggaaggaa cgcaaaatga gagtgatcat tctgttaaga tgattccttt gggaaaagga
    75181 tattccatcg agacattaaa aagtcctgtt gatgacgcgg caattttgaa ttatttaggt
    75241 gtttacaaaa aagacttagg aaaatttcta ggaatcaatg ttgatacgta tcaatcgctg
    75301 atgaagacag atattgaaaa agcgatgatg tatctgcaca acaaagcaat caaaccaata
    75361 ttgaaaaaca agagcgaaca ttacaccgct ctttttttta tgcctaattc tggctataga
    75421 gtggaatgga aaattaatat tttggatttt gttccttact caacaaaaac aaatattggc
    75481 tacaacatcg ttcgtaccgg gattacaagt ccagataacg tggcagaaat gcttggtttt
    75541 cctaaacaga atactccaga aacacaagct atctatattt caaatgactt atctaggatt
    75601 ggccagaaaa atgcaacaga tgattcctta ccaacgaatg atcaagactt gaaaggaggt
    75661 gatggaaatg aagaagaagg aaattcgcac gattgacatc accaatcttt caacacgttc
    75721 cgatgaagaa actcatacga ggaccattag cggctacgct gctgtattca acagtcaaac
    75781 acttttatgg gatgacttaa gcgaagtgat tgttcctggt gcattctcaa aggcaattag
    75841 taattcggat gttcgttgtt tgttcaatca cgattggtcc aatgtgctag gacgtactaa
    75901 aagcggaacc cttcgccttg aagaagatga acgtggtttg aaattcgaag ttgatttacc
    75961 ggacacaacg gtagcaaggg acttagttaa atctatggaa cgaggagaca tcaaccaatg
    76021 tagttttggt tttgtgccga ctgaagaaac gtgggactac aattctgaac caatgcttcg
    76081 aacaatccat gaagtggaat tatacgaggt ttctattgtg cctttgccag catacgaaga
    76141 tacagaagct gcattaagaa gtcgtgatga attagaaaaa aacgtcgaag aaagaaaaaa
    76201 attaatcaaa aaaattaatc aagcgctaga agcgtaggag gaaaaataca tggataaaga
    76261 attattgaaa aaaatgaagg cacgtcgtga gcaacgattg actgaattac gtgaaaaagt
    76321 tgaatcagga gaattacgcg aagcagattt agaagccgtg aaagaagaaa ttgacagtgt
    76381 tattgatgaa ttgaacggaa ttaaagacga attaggcgca gattctggaa ctgatgaaac
    76441 agacgacaat acagatgatc aatcgaatag tacggattca gacgaaagcc gttctggtga
    76501 agacaacgat caagaagaag attcagacag tgaagatagt tcagaaaatc gttctggaat
    76561 gattactcaa cagcaacgag atggattact tggatcaatt aagaacggat tggaggcacg
    76621 tgcaaaaatg accaagaaac aaaaagatca acaactacga aaagcatttg ctaattttgt
    76681 agctggaaat atttctgaag cagaagctcg agctttaggg attgaagctg gcaacggttc
    76741 agttactgtc ccagaagtaa ttgcatctga agttattact tatgctcaag aagaaaattt
    76801 acttcgtaaa tacggaacag tggtgcgaac atcaggagat gtcaaatatc caattcttgt
    76861 gaagaaagca gatgcgaatg taaacaagaa agagcgttca actgatattg ctgaaacagc
    76921 tattcagttt gatgaaattt tgcttaatcc tgccgaattc gatgctttgg caacagtaac
    76981 taaaaaatta ttaaaaatgt ctggtgttcc agttgaagat attgttgtgg aagaattgaa
    77041 aaaagcttat gtgcgtaaag aaatcaatta tatgttcaat ggtgatgacg ctggaaatga
    77101 aaatcctggt gcattagcca aaaaggctgt agcatttaaa aaacctttag atctaactgc
    77161 ttcaggtgct gggcaaaaat tatatgatgc attaatcgaa tttaaaaata caccagtgac
    77221 agaagtgatg aaaaagggac gctttattat taatcgagct gctttgactg ccattgaaaa
    77281 aatgaaaaca gatgatggat ttcctttgtt gcggccattc acacaagcag aaggtggaat
    77341 tggttaccaa ttagttggct atcctgtgga ttggacagat gcagcagata aaaagggtga
    77401 accagacacg ccagttttat attttggcga tttttctgca tttaaaattc aagaagttat
    77461 tggtgccttg gaaattcaaa aacttgttga aaaattctct ggtaaaaatc aaattggatt
    77521 ccaaatttac aacttgctag atggtcaatt ggtttattct ccatttgaac cggcagtata
    77581 tcgctacgaa attacaaaac cagttggtgg ttaagatgga agagcaaact aaagaattgt
    77641 ctttagagga aaaattcaaa tcacatattc attttgaaga gggcatggat gattctttgc
    77701 tctcttttta tttaaatatg gcaaaagatt atgtcaaaac agcaactggt ggccaacaag
    77761 aatatcttat tttgatggtt gccggcattg cctatgaata tagagtttca gaggatgaac
    77821 tcgacaaagc tatgaatgcc atgacgccat ttatcgtgca aggagcgatt caaaatgccg
    77881 aagagacaga ctaataatct cagatggaaa gccgaattgc tagacatcaa aacaggaaca
    77941 gacggaaacg atcgtccaac tacaatttac gaatttaagc gtctaatatt ctatgaagaa
    78001 ctcggtgtga cttctcaaga aaaatattta tcacagcaag ccaagacaga cgttgtcaga
    78061 cgaattaaag tcagatggga taaatccatc acagagaaat taagtgcgct caaaattgat
    78121 tctgtaacgt ataacattac tcgcatttat acgaatcccg atgcaagaga aatggagttg
    78181 agtttagctt atgtcgatta gctttgatga attgaaaaca gcgctgaaat caacaaagtt
    78241 agcagtgttc agagacaaag ccagattagg gacgatgtat ccatacattg tgtactcaaa
    78301 tgtgagtaac agcaaaaaaa tggcatccgg taaagtctat aaaaaattac cttattacca
    78361 agtctctttt tttacacttg gaacagaaca ggatttagtt gttattgaac aagcgctgca
    78421 aaatgctggc attccatatt cagattttac aggtatacaa ggtgatgaga acgacgatac
    78481 cgtaaccaac tattacacat atgtgaggtg tgtggaaaat gccaagtaat aagaatggtt
    78541 tttctgaaat atcggattat ttaggaaacc tttcaaaagt tgatccgaaa aaattatcgt
    78601 tggaatcttt agaagaagca gctaactttt atctaaagca gttgcttcct aatattccta
    78661 agtcgctctt aaaaaagaaa catatgcgcg atcaaataaa agtagtagtt gaaaacgatc
    78721 aagtgaaagt tcaatttgaa gagacggcct tttactggcg ctttgccgaa aatggcacaa
    78781 caaatcaacg tgcccaacat tttgcaagtg gtacgtatga acaaaacaaa gaaaaaattg
    78841 aagaaattat gactagaaaa atacttgatt tatgggaagg gtgattttaa ttgggaaaac
    78901 aagatacttt ttattttgaa ggattggacg acattttaat tgcgatgatg gcaacgccag
    78961 attctgttgg aactgcaccg acttatagtg aagttttacg attgccaatt gccacaaaaa
    79021 tcggtgtcaa agggaatgga acagcattag aaaaatgggc atccagcaaa atgtttcgtc
    79081 gtgtgtcgcg agaaacaaaa catgaacttg cgttggatca cgtgggtatt ccgatagaag
    79141 tgatggatga aatcaaagga ttagttgcta aaagcggagt aacgtttagc aaaaataccg
    79201 cacgagaatt tccgtacttc gcctttggat ttattggaaa tattgaaaat ggcggtaaaa
    79261 aagcagtttg gtatcctaat acacaactat ccaatgtgat tgacgaagag tatgccactg
    79321 cagaggaaga aacaaaaatt gacgatgtaa ctgcaaatct ggtttccatc ggactgaaat
    79381 acaataatgt gatgtatgca agttttgatt caaatagaga cggagcttct ctagaattat
    79441 ttaataaatt cattgctcaa cctgtatacg atgaagaaca gtggaaaaca ttggctaaag
    79501 ttggaggtgc gggttaatgg ctcggttatc tgattatgga attcacgttg aagacttaaa
    79561 aaattctgct gttgtaatta ttcaaggcgc agaattccct atttcattta ctatgcaaac
    79621 gatggaattt atagcagatg tgtatggtgg agattattcg caatttgaat ctgatatgaa
    79681 cgccatgcta tctaaaaaag aaggacaaat ttcttctgct aacttatcgc ctagtgatct
    79741 aaaaatcatg cgtgccttga tttatgccat gctgcgtact ggtggtttag aggaagatcc
    79801 agaaactatt ttcaaatttt tgggaatgag tggagaggtg ttgtctgctt atagtacctg
    79861 tatggaaatt tttgctagcc agacatttca ggtggaagac ctaaaaaaat ccaagaagcc
    79921 acaagacttt caaaaagcgc aagcaaaaag aaagaaaaac aaaaagaatc ggaagagata
    79981 ggaactcctt ggagttttta tatttacgtt gctctcactc tattgaattg gagtgagagt
    80041 ttctttttaa agtctacacc taacttgtgg ctcaaatctt atttacagtg gttacaacaa
    80101 aacaccgatt ttgaaccacc tcaatctgta actatggata aatcgccttg gtggtagaaa
    80161 ggagcgctaa gataaatggc tggtaaagaa tctgatgtcg ttcttaattt taaaacgaat
    80221 ggcgaagtca gttattcgaa aacaatcaaa gaaatcaaca aagaaatgaa cttagccgct
    80281 gccgagtaca aaaaccaagt gtctgcgatg gacaaggatg caactcaaac agaaaaattg
    80341 cgagcggcta aacaaaaatt agaaaaacaa ttaggattgg ctgaacaacg atctcaaatg
    80401 ctgagagagg aatatgaaaa atctgtcaaa gaaaccggtg aatattctgc tgaatcagaa
    80461 aaactttata aacaattact caattctgaa accggagaaa ataaacttcg tacagcatta
    80521 gaacagacga acgatgcact taaagaacaa ggtgacgttt ctgttgatac agcaaaaaaa
    80581 ctccagaaaa tcgaagaaac aggtgagaaa gtaaaaggcg ttggtgaaaa aatgtctgtt
    80641 ggagtaaccg cgcctattgt agcggcagga gcagcaggac ttgcagcatt tggtgaagtt
    80701 gacgaggcac ttgataccat cattacaaaa accggagcaa caggtgatca agctgataga
    80761 ctttcacagt ctttcaaaaa cgttggttca aatactcatt tacctttaca aacggttggg
    80821 gaagctattg gtgaggtaaa tacacaattt ggattcatgg ataaaaaact ggaagattca
    80881 accaattatc tcctacagta cgctgaaatc aatgatacag atgtttcgca atcagcaata
    80941 tctgctcgac aagctattga tgcttatgga ctagaatatg atgatttgaa ttctgtcctt
    81001 gatgtaacaa cgaaaacatc gcagaatact ggtcaatctg tagacgactt gatgcaaaaa
    81061 gcaattgatg gcgcaccaca aattaaacaa ttagggttga gctttggtga aggggtcact
    81121 ttacttggac aatttgagca atctggtgtt gattcaagcg cagctttaag tagtttatct
    81181 aaagcaacag tggcttatgc gaaagacgga aaatctttaa gtcaaggact agaagaacta
    81241 caagacaagg taaaaaatgc aggttctgaa acagaagcca tcaatgcagc agcagaagta
    81301 ttcgggacta aaggcggtcc tagaatggcc gatgcaattc gtagaggtac tttgaacctg
    81361 gaagatttag caaaaactgc tggagaaagt gggggagctg taggagacac attcgatgct
    81421 actctcgacc caattgatca agccgatcaa gctatgaaca atgcaaaatt agcaatggct
    81481 gacgtaggtg aagcagtcca agtaagcctt ttgccttttt ttgaaaaagc aacttcctta
    81541 ttacaggaat tctctaaatg gtgggggtct ctagatcaag atacaaagaa ttggatcata
    81601 acaattgctg gtatagcggc agcagttgga ccagtactaa tcgttcttgg ttcacttatg
    81661 ggatctgtta caaaaatcgt tggggaagtt aaaagtttta taggagtttg gcaaggactt
    81721 gctggtttat ttggaatgtc gggaggctgg tttgcattag cagttatagc tattggcgca
    81781 ttagttactg gactaatttg ggcatataat aacgttaaat ggtttcatga tggagtgaat
    81841 tctttcttca aaggtgtttc agatgtagct gttgaaatat ttaatttttt aagtggttat
    81901 tttggtggat ttttcgatgg tgttatagca aatttcaata atttttataa tgctggaaaa
    81961 cgaattttca gtggttttct agattttatc actggagtat ttacagggaa ttggtcaaaa
    82021 gcatggcaag gtctagttga tatttttgga ggtattttcg atggtattgt tgctgtaggg
    82081 aaagctccta ttaatgccat gattggattg atcaatggat ttattggtgg attgaataat
    82141 ataaagatac caaaatgggt tcctggtgta ggtggaaaat cattttctat ttcaaaactg
    82201 ccttatttag cacaaggcgg tcatctaatc aatggacaag cgattgttgg tgaagctgga
    82261 cctgagttac tgactgccaa gaatggtaaa accacagtta ctccattatc agacgaagaa
    82321 aaacgcaaag ggatcagtgg aagagtttct ggaggtagca ttgaacagca tatacacatt
    82381 ggcaaagtcg atgcaaacaa tccatctgag ttagatcgta tgaatcgtaa atttgctaaa
    82441 gcaaacaaac aggctattcg tgatttggga ggtgttccta tttgagtcga caatttatga
    82501 atccagatga accaaatttt atttggaaaa atcggaatgc agtaattgat atgaattgca
    82561 tcattgaggc agagcttcca gatattatgc cgaacaagcg atacgaaact tacacgattc
    82621 agggccgaag cggagaattg acggagacgt ttgatgacta tgaacctttt gatttggaaa
    82681 tagaagggat cactattcca cattcgaaac tgagagaagt caaacgatgg ctcactggta
    82741 aaagccaatt aatcacacat aatgatccag acaagtatct agaggctatc tgcagcatgg
    82801 ataaagaagt ccctttcgaa aacgagtggg gcttctttta tacattcgat gttacttttc
    82861 gttgtcagcc actcaaaaga aaaatcggtg aagcacctaa aatttttcat tctagtgtta
    82921 tggatatttt tgatcatggt gacgaaattg cacatcccta tctggaaata gaatcgaacg
    82981 gaggagatat aacgatcgct gtcggtgata aaagcctaac aatacttaat acgttagctg
    83041 caacaatcgt ggttgatact cagcttggaa aagcaataca agaaggtttg aatttattta
    83101 cgcgtggtga ttggccagta ttgcagcctg aatggaatca ggtaaaaata tccggaaaat
    83161 ttaaagaagt gcggttttgg aataggagtg tgtatctgtg acgcaagaat ttatttatgc
    83221 ttataaaaaa atgccagatg atttgagcgt gaacggagcg tcgttagttg attgggaaga
    83281 tttgccagaa atcaatcgtg tgctgaatgg ccaatatcgt ttttatggta actattcaag
    83341 aagcggacag tatcgctctt atttaaaaaa aggaaatttt ataaaagcga aggtaccaga
    83401 tggatcatgg caatattttg aaatttataa tgtcaaaaag aatttgacct ccgtttctgt
    83461 aacagctcgg catattggtt ttatggccaa taagaacttt attgtcaagt cgtttacgga
    83521 taacggcaat gggtctcaga tcatgaccaa tcttaaaaac agtttggcat ttgatcaaaa
    83581 atttaactat ctttcgaatg tagggacaac gcaccaattt acagcaagac aagtcgcgcc
    83641 agttgaagca atcattggtt caaataacgg taatcaaaat ttgactggcg tgactagcgc
    83701 ggaacttgat atggataact atgatttgaa actggtaaaa cagattggat cagataatgg
    83761 ttttcggatt gattttggca tcaatttaga agcaatcgaa gaagaaattg atgaagaatc
    83821 aattgtcaat agtttgtatt tggttggcgg tgttcctgat aacgattatg atgaagacaa
    83881 agaacctatc gaatatggtt atttagaaat tgatggagta accaacgaga atcgacgtat
    83941 tgccaaacgt gaaaacggag attgtaaaac agttgatgaa ttgatcaaat ggggcaaaac
    84001 cttatttgac aatgatcgta tccatgagcc aaaagctact catacagtca gtatggtggc
    84061 gttggaacac acactcgaat acggtgacat gtatcaagag ctagcgtctt tacactttgg
    84121 tgatgtcgct catgttaggg cgaaagagct agatatcgag ataaaagagc gtatggtcga
    84181 atacacttat ttcccaacgc ttgggaaata taaggatatt gttttaggta atgatttatc
    84241 actgtacact tcggcagtaa atactcaagc acaagaattg aaaaagaaaa tcgacaatcg
    84301 aacagaaacg ttagttcaaa atgtgttgaa tgcgacggca tggatcactg gtaattctgg
    84361 tgggcatgtc gtttttcgtc cagaaaaagc tccatcagag attcttatca tggatactcc
    84421 aaatgtggcg aatgcaaagc gagtgtggcg ttggaattta aatggcttag gttattcaga
    84481 caacggagta aatggcccgt tcggtattgc tatcacatca aaaggagaaa ttgttgctga
    84541 cttcatcaaa gtaggaacaa taaatgcaga agtattcgaa acttccttta atgcttatgg
    84601 tgatgtgtta aaacttgtaa aaggcacgct tcaaatttgg aatgaaaaca aaaaaatcat
    84661 ggagctaacc aaaaaaggta tggaattctg gaatagaaaa gaatccattg gaacgatcgg
    84721 aacaacagat tcagcaggaa atccatttcc aaatgctgta acacctacac ctcttgagga
    84781 aaattctttg gtaatacgta caaatggcga tggaaaatac attttaattt ctccaactgc
    84841 agaaaaagga tttgtgttat taggaaacgg aaaagcatat tatttcggtg atttagatat
    84901 tcagggaaaa cttactgtta gaggtaaaga agtgatacca ggacaaaatg gcggtccatc
    84961 tggcggagga gaaacacctg gtgggtatcc tgatgaatta aaaacagatg cagaaaagag
    85021 agcttggaga at
//
LOCUS       DAZBTF010000004        75448 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000004 DAZBTF010000000
VERSION     DAZBTF010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 75448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.004"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            32..664
                     /locus_tag="ACHGNS_000306"
     CDS             32..664
                     /locus_tag="ACHGNS_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936288.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(798..923)
                     /locus_tag="ACHGNS_000307"
     CDS             complement(798..923)
                     /locus_tag="ACHGNS_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFN6936289.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1057..1950)
                     /locus_tag="ACHGNS_000308"
     CDS             complement(1057..1950)
                     /locus_tag="ACHGNS_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFN6936290.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(2132..3058)
                     /locus_tag="ACHGNS_000309"
     CDS             complement(2132..3058)
                     /locus_tag="ACHGNS_000309"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFN6936291.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(3143..3904)
                     /gene="pflA"
                     /locus_tag="ACHGNS_000310"
     CDS             complement(3143..3904)
                     /gene="pflA"
                     /locus_tag="ACHGNS_000310"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFN6936292.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(4148..6379)
                     /gene="pflB"
                     /locus_tag="ACHGNS_000311"
     CDS             complement(4148..6379)
                     /gene="pflB"
                     /locus_tag="ACHGNS_000311"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFN6936293.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6656..9106)
                     /gene="parC"
                     /locus_tag="ACHGNS_000312"
     CDS             complement(6656..9106)
                     /gene="parC"
                     /locus_tag="ACHGNS_000312"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFN6936294.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(9118..11163)
                     /gene="parE"
                     /locus_tag="ACHGNS_000313"
     CDS             complement(9118..11163)
                     /gene="parE"
                     /locus_tag="ACHGNS_000313"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFN6936295.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(11329..11763)
                     /locus_tag="ACHGNS_000314"
     CDS             complement(11329..11763)
                     /locus_tag="ACHGNS_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFN6936296.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11890..12528
                     /gene="plsY"
                     /locus_tag="ACHGNS_000315"
     CDS             11890..12528
                     /gene="plsY"
                     /locus_tag="ACHGNS_000315"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFN6936297.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12660..13535)
                     /locus_tag="ACHGNS_000316"
     CDS             complement(12660..13535)
                     /locus_tag="ACHGNS_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFN6936298.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13617..14408)
                     /gene="codY"
                     /locus_tag="ACHGNS_000317"
     CDS             complement(13617..14408)
                     /gene="codY"
                     /locus_tag="ACHGNS_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFN6936299.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(14426..15826)
                     /gene="hslU"
                     /locus_tag="ACHGNS_000318"
     CDS             complement(14426..15826)
                     /gene="hslU"
                     /locus_tag="ACHGNS_000318"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFN6936300.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15840..16388)
                     /gene="hslV"
                     /locus_tag="ACHGNS_000319"
     CDS             complement(15840..16388)
                     /gene="hslV"
                     /locus_tag="ACHGNS_000319"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFN6936301.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(16425..17330)
                     /gene="xerC"
                     /locus_tag="ACHGNS_000320"
     CDS             complement(16425..17330)
                     /gene="xerC"
                     /locus_tag="ACHGNS_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFN6936302.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(17528..18904)
                     /gene="trmFO"
                     /locus_tag="ACHGNS_000321"
     CDS             complement(17528..18904)
                     /gene="trmFO"
                     /locus_tag="ACHGNS_000321"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFN6936303.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18873..20951)
                     /gene="topA"
                     /locus_tag="ACHGNS_000322"
     CDS             complement(18873..20951)
                     /gene="topA"
                     /locus_tag="ACHGNS_000322"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFN6936304.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(21073..21930)
                     /gene="dprA"
                     /locus_tag="ACHGNS_000323"
     CDS             complement(21073..21930)
                     /gene="dprA"
                     /locus_tag="ACHGNS_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFN6936305.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(21986..22753)
                     /locus_tag="ACHGNS_000324"
     CDS             complement(21986..22753)
                     /locus_tag="ACHGNS_000324"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFN6936306.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22746..23606)
                     /gene="ylqF"
                     /locus_tag="ACHGNS_000325"
     CDS             complement(22746..23606)
                     /gene="ylqF"
                     /locus_tag="ACHGNS_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFN6936307.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            23866..24264
                     /locus_tag="ACHGNS_000326"
     CDS             23866..24264
                     /locus_tag="ACHGNS_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN6936308.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            24501..25043
                     /locus_tag="ACHGNS_000327"
     CDS             24501..25043
                     /locus_tag="ACHGNS_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFN6936309.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(25126..25677)
                     /gene="lepB"
                     /locus_tag="ACHGNS_000328"
     CDS             complement(25126..25677)
                     /gene="lepB"
                     /locus_tag="ACHGNS_000328"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFN6936310.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(25829..26056)
                     /locus_tag="ACHGNS_000329"
     CDS             complement(25829..26056)
                     /locus_tag="ACHGNS_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFN6936311.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(26053..26571)
                     /gene="msrA"
                     /locus_tag="ACHGNS_000330"
     CDS             complement(26053..26571)
                     /gene="msrA"
                     /locus_tag="ACHGNS_000330"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFN6936312.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(26591..27304)
                     /locus_tag="ACHGNS_000331"
     CDS             complement(26591..27304)
                     /locus_tag="ACHGNS_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFN6936313.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(27307..28203)
                     /locus_tag="ACHGNS_000332"
     CDS             complement(27307..28203)
                     /locus_tag="ACHGNS_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFN6936314.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(28361..29203)
                     /locus_tag="ACHGNS_000333"
     CDS             complement(28361..29203)
                     /locus_tag="ACHGNS_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFN6936315.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            29335..29988
                     /locus_tag="ACHGNS_000334"
     CDS             29335..29988
                     /locus_tag="ACHGNS_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFN6936316.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(30051..30578)
                     /locus_tag="ACHGNS_000335"
     CDS             complement(30051..30578)
                     /locus_tag="ACHGNS_000335"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFN6936317.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(30598..31545)
                     /locus_tag="ACHGNS_000336"
     CDS             complement(30598..31545)
                     /locus_tag="ACHGNS_000336"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFN6936318.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(31557..33440)
                     /locus_tag="ACHGNS_000337"
     CDS             complement(31557..33440)
                     /locus_tag="ACHGNS_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFN6936319.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            complement(33603..35681)
                     /locus_tag="ACHGNS_000338"
     CDS             complement(33603..35681)
                     /locus_tag="ACHGNS_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFN6936320.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(35694..35969)
                     /locus_tag="ACHGNS_000339"
     CDS             complement(35694..35969)
                     /locus_tag="ACHGNS_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936321.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(36331..36786)
                     /locus_tag="ACHGNS_000340"
     CDS             complement(36331..36786)
                     /locus_tag="ACHGNS_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936322.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(36940..38148)
                     /locus_tag="ACHGNS_000341"
     CDS             complement(36940..38148)
                     /locus_tag="ACHGNS_000341"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFN6936323.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(38260..38592)
                     /locus_tag="ACHGNS_000342"
     CDS             complement(38260..38592)
                     /locus_tag="ACHGNS_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFN6936324.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            38740..39615
                     /locus_tag="ACHGNS_000343"
     CDS             38740..39615
                     /locus_tag="ACHGNS_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN6936325.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(39697..40278)
                     /locus_tag="ACHGNS_000344"
     CDS             complement(39697..40278)
                     /locus_tag="ACHGNS_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFN6936326.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(40284..41543)
                     /locus_tag="ACHGNS_000345"
     CDS             complement(40284..41543)
                     /locus_tag="ACHGNS_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFN6936327.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(41545..42591)
                     /locus_tag="ACHGNS_000346"
     CDS             complement(41545..42591)
                     /locus_tag="ACHGNS_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936328.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(42826..43101)
                     /locus_tag="ACHGNS_000347"
     CDS             complement(42826..43101)
                     /locus_tag="ACHGNS_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFN6936329.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(43344..44654)
                     /gene="der"
                     /locus_tag="ACHGNS_000348"
     CDS             complement(43344..44654)
                     /gene="der"
                     /locus_tag="ACHGNS_000348"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFN6936330.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(44836..46065)
                     /gene="rpsA"
                     /locus_tag="ACHGNS_000349"
     CDS             complement(44836..46065)
                     /gene="rpsA"
                     /locus_tag="ACHGNS_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFN6936331.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(46200..46880)
                     /gene="cmk"
                     /locus_tag="ACHGNS_000350"
     CDS             complement(46200..46880)
                     /gene="cmk"
                     /locus_tag="ACHGNS_000350"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFN6936332.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(46929..47564)
                     /locus_tag="ACHGNS_000351"
     CDS             complement(46929..47564)
                     /locus_tag="ACHGNS_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFN6936333.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(47630..49033)
                     /locus_tag="ACHGNS_000352"
     CDS             complement(47630..49033)
                     /locus_tag="ACHGNS_000352"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFN6936334.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(49020..50051)
                     /locus_tag="ACHGNS_000353"
     CDS             complement(49020..50051)
                     /locus_tag="ACHGNS_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6936335.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            50082..50303
                     /locus_tag="ACHGNS_000354"
     CDS             50082..50303
                     /locus_tag="ACHGNS_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFN6936336.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(50468..51205)
                     /locus_tag="ACHGNS_000355"
     CDS             complement(50468..51205)
                     /locus_tag="ACHGNS_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFN6936337.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(51358..52698)
                     /locus_tag="ACHGNS_000356"
     CDS             complement(51358..52698)
                     /locus_tag="ACHGNS_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFN6936338.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(52935..54458)
                     /locus_tag="ACHGNS_000357"
     CDS             complement(52935..54458)
                     /locus_tag="ACHGNS_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFN6936339.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(54836..55447)
                     /locus_tag="ACHGNS_000358"
     CDS             complement(54836..55447)
                     /locus_tag="ACHGNS_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFN6936340.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(55570..55695)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(55777..56493)
                     /locus_tag="ACHGNS_000359"
     CDS             complement(55777..56493)
                     /locus_tag="ACHGNS_000359"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFN6936341.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(56499..57068)
                     /gene="scpB"
                     /locus_tag="ACHGNS_000360"
     CDS             complement(56499..57068)
                     /gene="scpB"
                     /locus_tag="ACHGNS_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFN6936342.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(57052..57822)
                     /locus_tag="ACHGNS_000361"
     CDS             complement(57052..57822)
                     /locus_tag="ACHGNS_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFN6936343.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(57819..58190)
                     /locus_tag="ACHGNS_000362"
     CDS             complement(57819..58190)
                     /locus_tag="ACHGNS_000362"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6936344.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(58209..59096)
                     /gene="xerD"
                     /locus_tag="ACHGNS_000363"
     CDS             complement(58209..59096)
                     /gene="xerD"
                     /locus_tag="ACHGNS_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFN6936345.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(59192..59665)
                     /locus_tag="ACHGNS_000364"
     CDS             complement(59192..59665)
                     /locus_tag="ACHGNS_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFN6936346.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(59770..60633)
                     /locus_tag="ACHGNS_000365"
     CDS             complement(59770..60633)
                     /locus_tag="ACHGNS_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFN6936347.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(60829..62928)
                     /locus_tag="ACHGNS_000366"
     CDS             complement(60829..62928)
                     /locus_tag="ACHGNS_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFN6936348.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(63148..64257)
                     /gene="rpoD"
                     /locus_tag="ACHGNS_000367"
     CDS             complement(63148..64257)
                     /gene="rpoD"
                     /locus_tag="ACHGNS_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFN6936349.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(64281..66122)
                     /gene="dnaG"
                     /locus_tag="ACHGNS_000368"
     CDS             complement(64281..66122)
                     /gene="dnaG"
                     /locus_tag="ACHGNS_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFN6936350.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(66304..68619)
                     /locus_tag="ACHGNS_000369"
     CDS             complement(66304..68619)
                     /locus_tag="ACHGNS_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFN6936351.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(68804..69664)
                     /locus_tag="ACHGNS_000370"
     CDS             complement(68804..69664)
                     /locus_tag="ACHGNS_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFN6936352.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(69723..70355)
                     /locus_tag="ACHGNS_000371"
                     /pseudo
     CDS             complement(69723..70355)
                     /locus_tag="ACHGNS_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            70522..71283
                     /locus_tag="ACHGNS_000372"
     CDS             70522..71283
                     /locus_tag="ACHGNS_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN6936353.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            71309..73900
                     /gene="mprF"
                     /locus_tag="ACHGNS_000373"
     CDS             71309..73900
                     /gene="mprF"
                     /locus_tag="ACHGNS_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFN6936354.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(74088..75275)
                     /gene="tuf"
                     /locus_tag="ACHGNS_000374"
     CDS             complement(74088..75275)
                     /gene="tuf"
                     /locus_tag="ACHGNS_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFN6936355.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
BASE COUNT        20531 a        15937 c        12941 g        26039 t
ORIGIN      
        1 tatatactta tcaatttaat ttggagatat tatggaaatt caacgtagaa ccactaattt
       61 tttacattta ggctcttttt ttgcaacttt gacgatctta ttttttctat gttattcata
      121 ccttaagacc tcacatgcgc cttctagtgc attaggctgg ttgcttttgg atcattctga
      181 tatcacagaa gccgaagaaa atccgttttt tttgattttg tcttctcttt gcggcttact
      241 gttttctgtt tatttcggtg cctatttttt tcatcgacat cctggtacgt taaacgaagc
      301 ttcaagcgta ttgattgcat ttaaaacgtt ttctttttta gcaattcttt tttattcgtt
      361 taatcacaat acgcttttct tctttatcat gaatggttta gtcgtgattt tatccatttt
      421 tacttatttt attttttcac tcatttttag ccaattgaaa tgtccgctct tctttcgatt
      481 agttcctgtc acctgtttta tttacagctt gttttccttt tttgtccttg ctttaatgcc
      541 actgaccaac ccttcgacga tccaagtcgc aggaaatctg ttggatatgc tcttcatcgt
      601 cagtctaggt gtatttatgt ggcaaagaaa aaacaaacaa aataaaaagc attttgaagc
      661 ctgactatta cgacttcata aatgcttttt tagttttaaa cacttcaatc taaaagaata
      721 acccaagatt ggataaatta ttaatgactc gccattcgtt tcttagtgat tctggattat
      781 caaatggact tcctaatcta atcagctagt tcttcccata aaatgccata cttgactgaa
      841 tcataatatt gattatttaa aaatctaact tttcttatca ctacttcctt tgtcattcca
      901 catttctcac catttttttg cattcctttg tttccagacc atgtagtgaa tctaataggt
      961 ggtagatagg caaaatactc aaataattct tttaaccata aaggaagaga aaggctcctc
     1021 ctgcaagagt cagtataatt tttgcttact gctctcttag gttttactca ctaaaactgt
     1081 tccatattct ttgatataag aaaatgcatg cggtataaat ttcttgctca gctcagaaaa
     1141 gatttgtcgt ttaagaaagg ccatttcctc cattgtcaat gaaggatcca atgttggtaa
     1201 ttcaatctca aaattgtttt tttcgacaat aggtaattct aatgtgaatg attcttcttc
     1261 ctttatagta ttcgtcacaa gtagagactg atggaaaata ctaggccggc gatttttata
     1321 tttattctta cgtatcctgt caacgataac ttctatttga tcaaaaaagt acgattcatt
     1381 tacgtctcca ataagttcgg ctaattcttt gaatgccttc gttacaggaa gctgattctt
     1441 ctgtacaaaa aagtaattag ccaaagtaag cgggaagtgt tctatagagg aaagtgtgat
     1501 aaaacggtac ttctcatttc ctgcagttgt cctcagcaat tctgtctctc ggctgtttct
     1561 tactccataa aaatacgctt ctttccgttc atcttcgaaa caatacgccc aaataacttc
     1621 tcccatcgct aatttttgtt catcaacaga gagtctcttc aattgttcag ctatggtttc
     1681 agcagccgaa gtggcttgtt gcaagtaatc tttaggatca aatattggaa agttcaaatg
     1741 atatcttcgt tcttccctct tgattaatcc tgcatcgatc aataaatcaa gaaaatggga
     1801 aaatttattt tgcgaaaatt cttttttcaa ttctcttaga atcgtttctt gttctcgatg
     1861 ttcatataga taatcggtca atgaagaaaa aagcggatca tcggtcagtt tttgataaaa
     1921 gttttcctta ttttttgaat aatagttcat atatgttctc ttttctatcc gttttatttt
     1981 acttaaaagg cttttacgta aaaaggagga agaacaagag tcataattga ctgttattct
     2041 cctccatttc ttcggccttt agtatgttct atgtttcttt ttatatcaat cgcttatcca
     2101 ttcattttga atgcttcata ctaccatttg attagctaaa tacagccgtc aattgaggta
     2161 cgacttgttt tttacgtgaa acgacacctt taagcaaagc acggttgttg gtcaaagttg
     2221 tttggaaagc ttcttctact ttttcttttc ctttaccagc aacaagtaat tctgaatcac
     2281 tgtctaagat gtttgtgaca actaatacga acagatcgta gccatttgct ttcatttcat
     2341 cttccattgc tgcttctagc tctgcttgtc gatccatcac ttcattaagg tccactgtat
     2401 tgacttgagc gatacgaacg ttggaactgc ccattgggaa actttttgca tccaaatcaa
     2461 gaagaaccgc agctgattta tcacttagat ttgttcctgc tttcaacatt tcaagtccat
     2521 aagtgtttag atcgacttca gcgattgctg caagttcttt tgcagctgct tcatcttcag
     2581 gcgtacatgt tggtgatttg aacaacaatg tatctgaaat gatagctgag agcatgattc
     2641 ctgcgatttc tttaggaatt gttacttggt tttctttata tagttttaaa ataatcgtac
     2701 ttgtacatcc aactggttct gcacgatagt acaatggatt agctgtttca aagtttgcaa
     2761 tacgatggtg gtctactaca gctaaaatat ttacttcgct gatatctgaa atactttgtt
     2821 ggaattcatt atgatccaca agcatgacat ctgcattttc tgctttagct gactctacta
     2881 ctcgtggtgc agatagctga aagtggtcta atgcgtattg tgtttcttca ttaggtgtac
     2941 ctagagcaac tgcttctgtt tctttaccta attcattttg caagtgtgaa aacgcaatgg
     3001 ctgccccgat tgcatctgta tctggatttt ggtgaccaaa aactaaaatt tttgacatgt
     3061 cattcgctcc tttgtgattt ctcctattta ataatagcaa aaaaacggaa agaagcaata
     3121 cttatcttct ttccgatgcg acctatcgcg ttaaatattt tgtatactct tctgtatgaa
     3181 gcaatcgttt agcattttcg acacgttctt ttgtaggtgg ttcgatccct tctaatggat
     3241 atttcaaacc aagtgtttcc cacttgtatt ttcccatcgt atgataagga agaatctcta
     3301 ctctgtctac atttgacaag gtttgaataa agttgtctag tcggatcaaa tattcatcat
     3361 aatcacttcg ctcaggaact agtacatggc gaatccaaac cggtttgttg atttttgaca
     3421 aatattgagc catttctaaa atattttcat tgtcccaatg cgttagtttt ttatgctctt
     3481 cattatcgat gtgtttgatg tcaaataaca gtaaatctgt atacttcatc aattcttgaa
     3541 aacgtgaaaa gaaaggttct tcatacgtaa aaggttttcc gcatgtatcc aatgtagtat
     3601 gcatcttccg ctcttttgct tttttaaata gttcgatcaa aaaatcgatc tgcaatagag
     3661 gttctccacc actaacagta ataccacctt tatctcccca gtaaggtctg aagcgttctg
     3721 ctttatctaa aagttcatct gctgtgtatt ctttccctcc gccaatattc caagtatctg
     3781 gattgtgaca aaattggcaa cgcatgcggc atccttgcat aaacacgaca aagcgcagtc
     3841 caggtccgtc tactgaaccg aaagtttcga tagaatgaac atagccgatt gttttttctt
     3901 ccatttaagt tcacatcttt tctttgattt cattttaagt acaagttagc tgtcgaagcg
     3961 aagctttctg aacaaaattt ttcgcaattg ccgcttcgtt atgtgtaaaa tttacaggga
     4021 cggaacatac aagtcctgcc cctgtaaatc gtttctttca aataagcata ctgattattc
     4081 gattttgatg cgatggctat tcaacgtcct ttcgacgcta agtcgaaaaa cttttttcac
     4141 gaacttttta cattctgtcg tgtgaagttc tcatcaagac atctaattgt tgttctcttg
     4201 tcaagtcacg gaatttaact gcataaccag atacccggat cgtcaagttc ggatatttca
     4261 ttggattttc cattgcatca cgcaataaat cattactgaa tacattgaca ttcaaatgat
     4321 aagctccttt tgagaagtat ccatctaaga cattacgcaa gttttcgatt cttgtatctt
     4381 gttcttttcc aagacctgaa ggattgattg tttgggtatt tgaaattcca tccaatgcac
     4441 agccgtaatt cattttagct gttgagttca ggctagctaa taacccactt tgttccgcat
     4501 tgtaacttgg atttgctcct ggcgcaagtg gttttccagc ttcacggcca tcaggagtat
     4561 ttcctgtatt tttaccgtaa acaacatttg aagtaatcgt caataatgaa gttgttggtt
     4621 ttgagttgcg gtaagtttta tgacgtttga tttgtcccat gaagtattcc aagatccatg
     4681 aaactaattc atctgcacgg tcatcatcat ttccgtattt agggaagtcg ccatccactt
     4741 caaaatcaac tgctattcct ttttcattac ggattgcttt tacttttgca tatttgatcg
     4801 ctgacaagct gtctgccgca tgagagatcc cagcaatacc tgtagcaaat gtgcgttgca
     4861 agtcagaatc catcaaagcc agctgagctg cttcataaga atatttatca tgcatataat
     4921 gaatgatgtt caacgtattg acatatagtt ctgctaacca atccatcata ttcatgaagt
     4981 tatgtttcac ttcttcaaag tctagtattt ctgatgtgat tggacgatat tctggtccaa
     5041 cttgggcgcc agtcacttca tcgactccgc cattgatggc atataatagt gttttagcca
     5101 agttagcacg agcgccaaag aattgcatgt cttttccgac aactgttcct gatacacagc
     5161 aagcaatcgc acaatcatca gaaccccatt gttcacgaag ctgattatca ttttcgaatt
     5221 gaatcgaaga actttcgata gcgattctgc tagcatatgt tctgaatcct tctggaaggt
     5281 gttcagaata caaaacagtc aagtttggtt ctggtgaagg tcccatattt gttaatgtat
     5341 gaagcagacg gaaatcattc tttgtaacta atgaacgacc atctaatccc atacctgcaa
     5401 tggataaagt agcccagata gggaatcctg agaataattg gttatattca ggtgtacgcg
     5461 cgaatttgac catacgtaat ttcattacta agtggtcgat caattcttgt gcttcttttt
     5521 ctgtgattac tccattatcc aaatcacgtt ggatatagat atcaaggaat gctgagatac
     5581 gtccgattga catcgcagca ccattttgtg atttgattgc tgctaaatat ccgaagtata
     5641 gccattggac agcttctttt gcatttgcag ctggtttaga aatatcatag ccatatttcg
     5701 cagccatttc tttcatttgc gctaatgcac gatattgttc agtgatttct tcacgtaaac
     5761 gaatcacttc ttcactcatt gtatgataac cagtcatatc atgatctttt ttcttttgtt
     5821 ccatcaaata gtcgattcca tacaatgcca cacgacgata gtcaccgatg atacgtccgc
     5881 gaccgtaagc gtctggaagc cctgtaatga ttttgttttt acgagcagca cgcatttcag
     5941 cagtataagc gtcaaataca ccttgattat gtgttttgcg ccagtcagtg aaaatatgtg
     6001 tcaattcatc gtttggtgta tagccattgc tttttagcgc attgttagcc atattgattc
     6061 cgccgaacgg catgaatgct tgtttcaaag gtttgtctgt ctgtagaccg acgatttttt
     6121 ccaaatcttt atccaaatag cctgcatcgt gtgaagtaat agttgatacg atttctgtgt
     6181 ccatatcata gacaccgtta cgctcgaatt ggatatcatt taacttttgc aattcatccc
     6241 ataatttttc tgttgcttct gttgggcctt ccaaaaagct gtcatcaccg cgatattcag
     6301 tgtagttatt ttgaataaag tctctagtgt ctacgccttc tttccatttg gtacctttaa
     6361 atccattcca ttgctccatt gtatggacct cctaaatttc tgttttatgt aacagttttt
     6421 acgtgattag tataacacag caccctatct ttgcaagggt tttcttgttt tttatttaac
     6481 aatttttatg ttttatttac atatatttaa atcaacgttg acataccaac atttaagcat
     6541 tgtgaacact ttcatctttt tttgcaaacg tataaggatt tattaaaaat gaatgaaaaa
     6601 gagacgccac ctgtattaat acagatggcg tctcttcatt aaaatactac aacagttacg
     6661 actcgatgaa aatctcagga gcatcgataa cttcgatgac ttctccacct tttttctcat
     6721 ccatcgcaaa tgatccattc gatgttcgat cacctatagg gaaatcagtt gggtcaatcg
     6781 ttaactgctg ccctttttga ttcatcaagg ttatttcttt tgatcttcct tccgacatat
     6841 agataatacg gtgcggattt tttttcagct cacgcatgac cgtcactccc cgcttcgcac
     6901 gtgaagtttg gctgatttct tgagcaagca tgcgtttaac agctcctcgc tgagtaacga
     6961 ttacgatcgg cgtatcacct tctgctaaca caagcagccc gttgataatc ctatctccat
     7021 ctttcaaatt catagacttc actccagctg cctttgctcc aacaacggga acatctgtta
     7081 aaggatagcg caaacccatt ccttgatgcg ttactaagaa aacatctaag cctggctgat
     7141 cttctttcag atacacagca atcagttcat cttcttccgc ctttagcttc atacagctga
     7201 gcggacggct cttataggtg cgccatggtt cgaactctgt cattcgtgtt tgcttgatca
     7261 atccattctt gctgatgaat acaaatgttt tctctgcttg aattttttga tatgggaaaa
     7321 cagctaaaat cgattcgtct acagacagat tggagattgt ttgagaaatg tgctcaccag
     7381 catctttcca tttcaaatcg ggtagttcat ggacagggcg gtagatcaca tttgctttat
     7441 ttgtgatcag aagaacatgg tccaatgtac tcaattcacc tgagtatagc aagtagtcgc
     7501 cttctcgcat cccgatttct tctggtttgg aagcactata agaacgaagg cttgttcgct
     7561 tcacatagcc ttctcgcgtc acactgacaa ttacgtcttc ttgtgccact aagacttgcg
     7621 tatcaatttt gatttcttcg atttcgtctt cgattgtcgt tagacgagcg gaagaataat
     7681 tttttttcac ttctcgcaat tcttttttca taacagagaa aagctctttg tcatttgaaa
     7741 ggattttatt caactctgtg atttgcgcaa tcaagctttc ggattccttt tgaagctctg
     7801 tgatatccgt gttcgtcaaa cggtataatt gcaatgtgac aattgcttct gcttgttctt
     7861 ctgtaaattg aaagacgtcg actaaattct ttttggcgtc ttttttgtct ttactttcac
     7921 gaatcgttgc gattacttca tccagaatcg ataaagcttt catcaatcct tctacgatat
     7981 gctgtctttt ttgagctttg gctaaatcga attgactacg ttttgttatg acttgctttc
     8041 gatgctcgat ataacttctt aaaatgtctt tcagtccaac ttgatgcgga gtcatatggt
     8101 cgatagcgac catattgaaa ttgtaattga tctgtagctc agtgtttttg aacaaataat
     8161 tcaaaattcc ttgagcattg gcgtcttttt tcaattcgac gacaatttgc agacctgttc
     8221 tatcactttc atctcgtact tcagctattc catcgatttt cttattcaaa cggatctcat
     8281 ccattttttt caccaaggtc gctttattta cttcatatgg gatttctgtg atcacgattt
     8341 gctgtttatt acccttcatt gcctctatct ttgttttcga acgaagaata acttttcctc
     8401 gtcctgtttc ataagctttt ttgatttcat cttttccttg caagatccct cctgttggaa
     8461 agtcaggtcc aggaatatat tccatcaatt tttcaaggct cgcttgtgga tgatcgatca
     8521 tatagattgt gccatcgatc acttcagcta agttatgagt cgggatttct gttgcatatc
     8581 cagcagatat acctgtcgat ccattgacga gaaggtttgg gtatcgagct ggaaggactg
     8641 ttggttcttt ttctgtatca tcaaaattcc acacgagatc gactgtttct ttctcgatat
     8701 ctgctagcat ctctccactt aacttggata atctggcttc agtataacgc atcgcagcag
     8761 gtggatcacc gtccatactc ccgttattcc cgtgcatttc gatcagtact tcccgcaact
     8821 tccagtcttg gctcatgcgg accatcgctt cataaatact gatgtcacca tgaggatgat
     8881 aattccccat gatatttccg acagattttg ctgatttacg gaatcctttg tcatacgtat
     8941 tgccatcttt gttcatggca aataatatcc ggcgttgcac tggcttcaat ccatcacgga
     9001 tatcaggcag tgcacgttct tggataatat atttggaata acgtccgaag cggtcaccca
     9061 tcacttcttc taaagttaat tcttgtactt cttgtcgatt ttccatcagg tgacctccta
     9121 ttctacttca aataactgat tgttttcatt tttatctgct cgttcttcat ctaataaatc
     9181 attagaaact gaaggagaga tttcagtatc ctctttttta tctaagatac tgccatcttc
     9241 ttcgagcgta aattgcacgt gattttcgat ccattttctt cgaggttcta ctttgtcacc
     9301 catcagtgtg gttactcgac gttcagcttg cgcagcatca tcgatacgga cgcggatcaa
     9361 cgtacgggaa gtcggatcca tcgttgtttc ccacaattgt tccgcattca tctctccaag
     9421 tcctttatat cgctgcagca tatatccttt gccgactttt ttaatcattg ctgccaattc
     9481 gtcatctgtc cacgcatatt caatgacttg cttctttcct tgtcctttgg atactttata
     9541 taaaggcggt aacgcgatat atactttccc agcttcaatc agcggtttca tataacgata
     9601 aaagaaagta agcagtaata cttgaatatg ggcgccgtct gtatccgcat cggtcatgat
     9661 gattactttg tcgtaattac agtcttcgat agaaaactca ggaccgactc ctgcaccaat
     9721 cgtatagatc atcgtattga tttcttcatt tttcaagata tcttgcattt tagctttttc
     9781 ggtattgatg acttttcctc gtaaaggtag aattgcttgg aactttctgt ctcttccttg
     9841 tttagcagat ccgccagcag aatccccttc gactaaatac aattcatttt tcttagggtt
     9901 tcgggattgt gcgggtgtca attttcctga aagaagagat tcgccttttt tacgcttctt
     9961 cccatttcgg ctttcttctc tggctttacg tgctgcttct ctcgcttctc tggctttgat
    10021 tgctttacgg accaacatct ggctcatttc gctattttcc tgcaaataaa agcccatttg
    10081 ttcactgatg acgttatcta ctacggtacg agcaactggc gtaccaagtt tttcttttgt
    10141 ttgcccttca aattgcagaa gattttcagg tacacggata gaaagtacag cagctaaccc
    10201 ttctctgaaa tcactacctt ccagattctt gtcccgttct ttcagcaaac caacttttct
    10261 cgcatattca ttataagatt tcgtcatcgc cgctttcata ccagcttcat gggttccccc
    10321 atcttttgtc cgaacgttat tgacaaaaga taaaacattt tctgaataac cgtcattgta
    10381 ttgataagcg acctctactt cgataccttc tttttctcct gaaaaataaa caactggtgt
    10441 caatgtatct ttttcttcat tcagataatc aacaaattct ttgattcctt cttcgtaatg
    10501 aaaaatttcc ttgactggtt cttctccacg aagatcagaa agagagattt taacgccttt
    10561 tagaagaaag gcagattctc ttaaccgttc ggctaaaatt tcatatgaaa atttcgttgt
    10621 actgaatatt gtatcatctg gtaaaaaatg gacagtcgtc ccgtttgctt tttttgtttt
    10681 tcctatcttt ttcaaagtac cgtcaggttt accgccgttt ttaaatttct gctgatactc
    10741 tacgccatct ctaacgatcg ttacaattag ccatttcgat aaggcattta ccacactggc
    10801 tcctacaccg tgcagtccac cagaagtttt gtaccctcct tgaccaaact ttccgccagc
    10861 atgcagcaca gtaaaaatca cttcaactgt tggtattccg gaagcatgca ttcctacagg
    10921 catcccacgt ccgctatctg caacagttat actattgtct tcatggatgg tcacatcaat
    10981 ctcactgcca taacctgata atgcttcgtc aactgcatta tcgacgattt catagactaa
    11041 atgatgcaag cctcggctgt cagtggatcc aatgtacatc cctggtcttt ttctaactgc
    11101 ctctaatcct tccaatactt ggatggacga gtcattgtat tcgttgttta ctttttttgc
    11161 caaggcaaac gctccttcaa taatactttg cggcaaaacc gcaccacgat tatcatacta
    11221 gaaaaggtcg tgaaaaaaaa gagggtagac gagaaatcaa ttttaattat ctcctaaccg
    11281 ctttttcgac attcccctct atcgatcatc tcttctttgt ggtcattttt atttttggtt
    11341 ttctttcaac ttaccaagtt caatcttaat acaacgatcc attacgatat ctgtacgtcc
    11401 tgcttttctt agcatctcag ctgcttcttt gttttcaatc cctaattgtg cccagaacac
    11461 tttagcatca gttttcaaaa aatcacctgc tatttcaggt aaaaattcac tccgtctaaa
    11521 tacatctacg atatcgattg gatcatcgat ttctgtcaaa ttcgcataga ctttctcgcc
    11581 tagaatttct tcaccagcca ataatggatt tactggtatg attttatatc cattgtcttg
    11641 caatacctgt gcaaccatgt aacttgttcg ttctggttta ttgctcaagc ctacgacggc
    11701 aatatttttt gcttcttcca aatagttgaa aatcgtttct tgtgttggat tttttacaga
    11761 catttctgcc accccttgct tttcttttta ttccatctat tatacttgta tctttcattt
    11821 tttcaattgt tcctgttacc tattcatgct aaaataaagg atagttttca acttgaaaga
    11881 gggtaaaaaa tgaaattgat cttattactt attttagcat atcttttagg ttctattcct
    11941 tccggtgtct ggattggcaa attgtttttc aaaaaagata tccgacaaca cgggagcggt
    12001 aatactggaa cgaccaatac atttcgtgta ttaggcaaaa cagccgggat tgttgtcctg
    12061 cttatggata ttttaaaagg tacattggct acttgtttgc cgatgatctt ccacctttct
    12121 gggattaacc ctctttggtt tggggtatgc gccattcttg gccatacatt tccgattttt
    12181 gctgggttca aaggaggaaa agctgttgcg acaagtgcag gaatgctttt aggatttaat
    12241 cctgtatttt ttgtttattc ttgtatcatc tttatcgtta gtttattttg tacaagtatg
    12301 gtgagtttga gcagtatgat cagtgcagta ttgatcactt tatctacgat cgtccttcct
    12361 tatgtagctc ctgtgatttt ggctaagccc aattggttac tgacgatcat tgccttttta
    12421 gtttcttcct ttatctttta tcggcataaa gacaatatca agcgtatcag aaaccatact
    12481 gaaagtcgaa tcccgtttgg tttgaatagc agcaagaaaa aatcctgata ctttttcatt
    12541 ccaaagagat aaaacgggag tgtgacaaat cgatgtcaca ctcccttatt acttacagga
    12601 tagctttttt acacaccttg gtaaacggtg ttccctgcta acttctcggt cacaacctct
    12661 tacttcactg tgatggaata ttttgtcttg aagatttcat gggcacctag ttgattgatc
    12721 cctacttttt ccatcaagtt tccactactg ttcaccgtgt cagcgattcc gcaccacggt
    12781 tcgatacaaa cgaaaggcgc ttcttttgga taaggagacc agatccctac ataaggcata
    12841 tctttatagc ttaaagttac gctatgggaa gatttttcac ttcgaatggc gtacgcattt
    12901 agaccccttg tttcaaaaac caaggcatct tgctcaaaaa gttcacgatt caaagccaga
    12961 ttcgtatttg tctgacctaa cgtcttttga tccatatcga tgaatgggcc tttcaatggt
    13021 aggcgtaatc ttgatttcaa tggagaaaat gccagataga aatcttcaaa tgacaatcct
    13081 tcttcaagag gtacattgaa agcaggatgt ccaccgatag aaaagaacat ttctttttca
    13141 ccggtatttg ccacttgata atgaaccgtg atgccgtctc ctcctaattc atagctgatc
    13201 actaattcaa aatcaaatgg atacttttct aatgtctccg gcgtactcct taagatgaaa
    13261 ctagccattt cttgtccatg ttcaatgact tcaaaatcca tatcccgagc aaatccgtgt
    13321 tgccccattt gatatgtttt cccttgataa acatactgat catcttttaa acgtccaaca
    13381 aaagggaata aaaccggtgc atgtctgccc caatacgctg gatcaccttg ccagatatac
    13441 tctaaaccat gttcttttga tttcagactg atcaattccg caccgtgttc agcgatttcg
    13501 gcgatcagct ggtcattttg aataataaca gtcattgcca ttcctccaac ttcgacttac
    13561 tttttaaaat gggaaggctg gaacaaacat ttctatctgc cccagtcccc ttacttctaa
    13621 taactgtgtt ttgctaactc gtctttaaaa cgggaattca atacttttaa ataagttccc
    13681 ttcatgccta acgaacgaga ttcaatgatt ccagctgatt ccaattttcg taaagcattg
    13741 acgatcacag aacgagtgat gccgatacta tcggcaatat tagaagcagt cagacgacct
    13801 tcatctccat ccaatgcttc aaagatagct tgtacagcct tcaattcgct ataagataat
    13861 gtgttgattg ccatttggac agccgttgca ctccgtacat ctgcttcgat gcttcgagac
    13921 ttctgataaa ggatctgcat accgacaacc gtagctccat attctgctaa gactagatcg
    13981 tcatctgcaa acgattgttc catacgagat aagataatcg tacccaaacg ttcgccagct
    14041 ccaaagatcg gaacgacagt cgtcagacca aatggatatt tctctcttga ctctaccgga
    14101 aatgcagtaa tatcactagt aattgggata ttagcttcag ttttcatcaa ctgtgccact
    14161 gtatcagtat aagattcagg aaattgtttc tcaacaaaca ttgttttgat tcgttcattg
    14221 ttcacatcgt gacgttcatt ataacctagt aatactccgt cacttcctat gatataggtg
    14281 ttgctgtcaa gaataccacc tagtgtgaca gccattttat cgtaaggcaa atctgcttgc
    14341 atgtcaaaag tattcttttg ctgcaaaagt gtgtttatct tgcgggtttt cgttagcaag
    14401 tctgtcattg tcgtcctccg attttttata aaatatagcg acttaaatct tcatttgccg
    14461 caattgagcc aagtttgtca tccacatatg cttctgtgat cgttatttct cccatttgca
    14521 tatctggtgc ttcaaataat agatcttcta gtagtttttc caaaatggta tgcagtctgc
    14581 gtgcgccgat attatctgtg tcacggttga catcgaaagc gatttgagcg atccgttcaa
    14641 ttgcttcttt tgtaaacgta actgacacat tttctgtacc gatcaacgcg acgtattgtt
    14701 ttactaaagc attatttggt tcggtcagaa tcttcacaaa atcttcagct gtcaaatcat
    14761 ctaattctac ccgtatcggg aagcgtcctt gaagttctgg aattagatca cttggtttag
    14821 acaaatggaa agctcctgaa gcaatgaata ggacgtgatc tgtttggatg atcccgtatt
    14881 ttgtgtttac ttgtgaacct tcgacaattg gcaatatgtc tctttggacg ccttctctgg
    14941 atacttctcc agattgctgt gatttagaag tgatcttatc gatttcatcg ataaaaataa
    15001 ttccactttc ttccgctaat ttgattgcct cactatggat atcagcatcc ttgactaatt
    15061 ttgctgattc ttccttgatc aataactcta aagcttcttt gactgttacc gtccgttcca
    15121 cttttttctt cggtgtcaaa gcacctagag tttcatttag gtcgatcccc atttgctcca
    15181 atccattgtt catcattggt gcaggtttct tttggtcttc aacttcaatg gttacttcac
    15241 gactatccag taaacccttt tgtagttgtt ccaagactgt acggcgattg attcgaatat
    15301 cttctgtcac ttcttcttgc ggttcagcgg tattttgctg actagcatta aaaagattca
    15361 tcatttgttc aaattgatta gtggattgtt tctgttcttt tttgatacca ggtaccaaga
    15421 tttttaccaa acgtttttct gcattttttt tagcacttgt gtatacacgg ctgtactgct
    15481 ctttttctac gatttggatc gcattttcta ctaaatcccg aaccattgat tctacatctc
    15541 ttccgacata accgacttca gtaaattttg ttgcttcgac tttgacaaat ggtgcattga
    15601 cgatttttgc caaacgtcgt gcgatttctg ttttaccgac ccctgtaggt ccgatcatca
    15661 gcatattttt tggtgtaata tcttgttgca ttttttcatc taactgcaaa cggcgatagc
    15721 ggttacgcaa agcaacggca acagattttt ttgctgcgtg ctgtcctacg atatattcat
    15781 ctaattcact tacgatttgt cttggtgttt gttttaattc atacatatct actcacctct
    15841 tacagttctt ctacgataat attatgatta gtaaagatac agatatctgc ggcaatattt
    15901 aacgcatttt ctgcaatttc tttcgcactc atgtcgtttt gagcatagtt tttcattgct
    15961 cgggctgcag ccaacgcaaa attccctcct gatccgatag ctaagatacc atcatctggt
    16021 gtgataactt ctcctgtacc tgaaacaaga agcatttctt tatcattcat tacgatcagc
    16081 atcgcttcaa gtttttgcat agattgctgt gtccgccatt cttgagctaa ttcgacagct
    16141 gcgcgttgta gattcccgtt atattcgttt agtttgcctt caaatttttc ttctaaagta
    16201 aacgcatctg caacacttcc ggcgaaacct acaacaactt ctccattgta gatacgtcgt
    16261 actttctttg cagtgccttt catgacaaca gattcaccca ttgttacttg gccatctcca
    16321 gccatagcaa atttcccgtc tttttcgacg gcacagatag tggttgaatg aaatggtgat
    16381 tcgaccatgt ttgtttcctc ctaaatttgt tcttttcctt atttttacgc tcttggatgg
    16441 aaagaacgat agtttttttg taaactttct tttgttacat gggcatagat ctgggtagtc
    16501 gataaattgg catgtcctag caattcttgg actgtccgca aatctgcccc attatttaat
    16561 aaatgagtag caaatgtatg gcgcagcatg tgaggatgga tatcactatc caaactgctt
    16621 tttttgatca tttgattcaa gacatattca attccagtag gcgtgattgg ttttccatga
    16681 tgattcacaa aaacgatatc atgctcttcg tgatattttg tcatcaatac ctggcggcct
    16741 tccctgaaat attcttgcag agcatcttga gcaaaagaac ctagaggagc atatctctct
    16801 ttgttccctt taccatggat caacatgacc tctgaatcaa aatcaatgtc tgataccgac
    16861 aaactgctac actcacttaa acggatgcct gacccgtaaa ggacttccag taaagcgcgg
    16921 ttcctcaaat ccaaaggact atctcctgct acgctgtcaa aaagtgcttg catttcattt
    16981 tcataaaaaa aacgtggcag tctcaaattt ttctttttga gatgaacgta agaaaatgga
    17041 ttttctttta tcacttcttc tttgagaaga tactgataaa acgaacgtag gctggctatc
    17101 ttgcgactga tagaatttct gctatagcta ggacgatgat cagctaatgc actcaaatag
    17161 acacgaaccg taaaatattc cgccttcagc agatcagcgt ctccagattc ctccaaaaaa
    17221 tgtttgaaat ggagaatgtc ttcttcatat gcttctttcg ttttttctga atatcctctt
    17281 tcgacaatca aataacgcaa aaatttttgt tcccacgtgg cttctgtcat tttctcacct
    17341 tccttttgtc ccagagaata atttagcaca gcgtttataa aaagacaaat aaattgtcag
    17401 aatttaacca atatttctaa gtttgttcac aattggcgca attcctcaca ttttgtttga
    17461 gaaattctta agaaaaacgc aagaaaaaga gcgagactat ccgtctcacc ctcttcatgg
    17521 agtttcctta ctcatttggg attgcagttt tttctaattc tgtttttatt gcctcattag
    17581 cttttaaagc tcgttgtgca attgcttcat atcttgcttt tttgtctcga atgcgctctg
    17641 gcagttcagg aaacagacca tagttggcat tcatcggttg gaaatgtttt ccttccgcat
    17701 gagtaatgta gtaagccata cttccaatag ctgtttcttg tggaaactcg actggttcca
    17761 tttcttttgc taagcgtgcg gcgttgattc cagcaagtag tccgctcgct gcactttcaa
    17821 catagccttc tacaccagtc atctgacctg caaagaacag atcttcgcgt ttcgttgatt
    17881 ggtaagtcgg tttcagcaat tctggcgaat tcataaagct gtttcggtgc atcacaccat
    17941 agcgaacgaa ttcagcattt tctaacccag gaatcatccg gaaaacacgt ttttgctcgc
    18001 cccatttcaa gtgagtctga aagcccacga tgttgtataa agaagctgca gcattgtctt
    18061 gacgcagctg gataacagca taaggacgtt tgccagtttt aggatcttcc aatccaactg
    18121 gtttcatagg tccgaaaagc atcgttttga atcctcgctg tgccattact tcaattggca
    18181 tacatccttc aaaatatttt tctttctcaa aagatttaag ttctgccact tcggcattga
    18241 ttagtgcctc atggaaagct ttgaattcct cttctgtcat cggacagttc aagtaagctg
    18301 cttctccttt gtcatagcgg gacttcaaat atactttatc catatcgatc gtagctttgt
    18361 ctacgattgg tgcagctgca tcatagaaat aaaaaccatc tgaaccattg aatgctttga
    18421 tgctttctgc taatggatga gaagtcaaag gtcctgttgc tactacggtg attccttccg
    18481 gtatttcagt aatttcttcg tttatcactg ttactaatgg atgctctttg acttttcggg
    18541 taattgtttc agaaaaagaa tcgcggtcaa cagctaaggc accaccagct ggcactgctg
    18601 tttcatctgc cgaagtgatt actaccgaat tcaatcggcg catttcttct ttcaataacc
    18661 ctactgcatt agccaaactg tttcctctta atgaattgga acatactagt tcagcaaaat
    18721 tacttgtgtg atgcgcctca gtcgatttga ctggacgcat ttcatataaa cggacaggga
    18781 ctcctgcttg tgcgatctgc caagcagctt cacttccggc aagccctgct ccaacaacat
    18841 ttaccatttt caatgaaaga acctcttttc tattattttt ggatgtcttc ttcgtaatct
    18901 ccatttacac agactacttg tttaccgcct ttgactttct tctcgaccag atatccgtca
    18961 cattttggac aatttctgcc cactggttta tcccaagaag tgaattcaca ttctgggaaa
    19021 cgggaacaac catagaatag tctgtttttc ttagattttc gttcaatgac ttgtccttca
    19081 tgacatactg ggcaagtcac accgatttct tttacgattg gtttcgtatt ccgacaatca
    19141 ggaaaattac tgcaagcata aaacttccca tatttcccta attttatcac catcggatgt
    19201 ccacagacat cacaatcgaa tcctgctggt tcatctttga tttgaatttt ttcgattttt
    19261 tcttccgcat tcgtcagttc tttttcaaac ggtttataga aacgatcaac cactttgacc
    19321 cacgcttccg tgccttcttc aattttatcc aaatctcctt ccatttctgc tgtgaaatgg
    19381 atatcgacga tttgagggaa gaattcacag attaatgaat tgacaatttc tcctagttca
    19441 gtcggctcaa atcttttttg tgtcaatttt acatagtagc gtctttggat cgtatcaagt
    19501 gttggtgcat aagtggaagg tcgccctacc ccgttttctt ctagtgcacg aatcaacgta
    19561 gcctcactaa aacgtgctgg tggttgagta aaatgttgtt ttggttcaat atcaacagat
    19621 tggactacat cgccttcttc aagttctgga agaatatttt ctttctcttc tttgccgtca
    19681 tctctacctt ccacatacac ttgcatgaat cctttgaatt tgatttttga accgttcgca
    19741 atgaagatga caccattttg ttctaatgtc actttcattg tatctaaaat agctggtgtc
    19801 atctgactag ccacaaaacg tgaccagatc aatgtataaa gtttcaattg atctttatct
    19861 aaatattgtt tgagttcatc tggtgtacga agcacactcg ttggacgcac agcttcatgg
    19921 gcatcttgtg cgccttgagc atttttgatc tttcggtgat catgtctaga aaattctttt
    19981 ccatacgtat cttcaataaa tgctgcggcc tctgcttttg ctgtatcggc aatccgggta
    20041 gaatcagtac gcatgtatgt aatcaagccg actgttcctt gtttacccaa agtgattcct
    20101 tcatataatt gctgtgctac catcatggtt tttcttgttc gaaagtttaa cttacgggca
    20161 gcttcttgtt gcaggctact tgtagtgaaa ggattcgcag ggtttctttt gcgctctttc
    20221 ttttctactt ttttaacttc gaagtctgtt ccatcaattc tagcagtgac ttcttttaca
    20281 ctttgtgcat ctggtaattt ctttttctta ccgtcgattc cccagaaatt tgctttgaat
    20341 ttcttgcgag cttttttaaa attaccgtcg atgctccaat attcttctgg aacaaattct
    20401 cggatctctt tttcacggtc aataataatt tttaaagcga cagattggac acgtcctgca
    20461 cttaaccctt tcttgacctt acgccagaga ataggactga tcgaataacc aacgattctg
    20521 tctaaggtac gacgagcttg ctgtgcatct actaaatcta catcgatcgt tcttggttcc
    20581 ttaaaagctg ctttgactgc gtcttttgtg atttcattga agaccacccg atttttttct
    20641 ttcagatcca acccaagtag gtacgctaaa tgccaagcaa tcgcttctcc ttctctatcc
    20701 gggtcacttg ccaagtaaac tttttgagct ttttttgctg cggctttcag gcttttgatg
    20761 acatcgcctt ttccgcgtat agaaatataa tggggttcat aattgttttc aaaatcgata
    20821 cccattttac ttttcggtaa atctcttata tggccaacac tggccattac tttatagttt
    20881 cgtccaagat atttctcaat tgtcttggct tttgcaggtg attctacaat cactaaatat
    20941 ttatacgcca ttactgaatg gactcctttc cattgtcgtg ttcctactat taaaagagga
    21001 ttcatacaaa tcgtagctaa tcatatccat tcagaaaacg tttgtcaaga aaagcaaatg
    21061 aaaagtatgg tgttattctt taaattctgc caagatatct tcacccgaaa ccgtgcaaac
    21121 tgcaccgtct tggatcaaat gatgacatcc ggtagaatgt tctgttaaaa tatttcctgg
    21181 aacagaaaaa actgttcttc caagatccaa tgcttgctgt gctgttatca gagagccgct
    21241 ttttttcttg gcttcaaaga cacatgttcc tttagacaaa gctgcgataa tccgatttct
    21301 cattggaaaa caatgctttt tcgggccgga ttgaggagga tattcactca aaagaagatg
    21361 atcatttgca atcacctctt gcaagtcacg attttcagca ggatagactt gctctatccc
    21421 tgttccaatg actgcgattg tccgtccacc atgagagatt gctgcttggt gaccgtatgt
    21481 atctatccct ttcgcgcagc cactgacgat cacatatcca gcttctatca ctggttttag
    21541 taaataataa actgtttttt tggctaaagc agaagtgtgt cttccaccaa caacgcttaa
    21601 catgttacaa gaaagtaaag atagatcacc attataaaac agtacaattg gcggataggt
    21661 caagtgaagc agctgagggg gatatttggg atcaaagcaa gtgataaacg actgtttcga
    21721 ttggattttg ccccagttag cgtgaatttc ctgccaactt ttgcgaaaca gctcccggtg
    21781 tttggttatt ttacctatct taatgatgtc ttcaaatccg atttctttat aaggatactg
    21841 catcatccag ttagcaagtg cccattttcc ttgactactg attccttttg tataagcaag
    21901 tttcaataaa ttttcttcta tttgatacat cataaaaccg cctttcgtct ctctttctaa
    21961 taagatacgt aaaagcggct gttttttatt gtaaatattt ttttacaggt gcaaaacttt
    22021 tacgatggat tggcgtaatt ccttgtcgtt caagtccttc taagtgttcc tttgttccat
    22081 aacctgcatt atgcgagaaa ccatatccag gataaagcac gtcatattct ttcatcaatt
    22141 ggtctcgata tactttagca atgatacttg cagcagcaat cgacacagat cgtgcatccc
    22201 ctttgattat tttttcctgc tgaataggca aatccaaggt catcgcatcg atcaacaaat
    22261 agcttggtac aatggacaat ttttctactg cttggatcat cgctaattta cttgcttcat
    22321 aaatattcac atcatcgatt gtctgctgat caacgatccc gatcccgatt gccagggctt
    22381 tttcttgaac ttgttgataa agaagttctc gctttttctc tgataattgt ttggagtcat
    22441 tcagccctaa tatgatctca tttttcggca agataactgc agctgccacg acaggtccag
    22501 ctaatggtcc tcttcctact tcatcaatgc cggcaatcca ttggtgacct tgtgcgtacc
    22561 cttgcttttc aaagcaaaac atttcttcat atttttcctt aattaatgct tcctttttca
    22621 atcgtcgttc aaaagaagcc agtgcttgct gcacgccttt gcgtggatca tttcttaatg
    22681 attctagtgt aggatcatct attctctgta tgcttttcaa tcgttctttg atactttgga
    22741 tggattcagt catcactttt ttgctccatt tcttcgcaac gatccagtgt gtatcgtcca
    22801 agttttcctt gacgaatatc atgcacgact aattcgctgc cacgatcata gtcttcttta
    22861 tacccacgtt ttgctgtaat atccatcagt aattccggtg ccaatttttc ttcttgctcg
    22921 attgataaac cataacgatc gctgattctt cctgaataat atcgggcaaa aaagtctaat
    22981 ccgtaaatcg ctaaatcatc taaatggaca agctggtctt tgatcgcgcc tgtcagcgct
    23041 agttttttgc cgatttcttc atcttcaaat ttgggccata aaatccctgg tgtgtctaac
    23101 aattccaact cattacctaa acgaagccac tgctgacctt ttgttacgcc aggcttattt
    23161 cctgtctgtg caattttctt gcctactagt cgattcatca acgtagattt cccaacattt
    23221 ggaataccta agaccattgc ccgaatagcg cgaggcttga tcccccgagc agcatctctt
    23281 gcccgttttt ctttcaatgc ttctttagcc gcaggtacta gacctttaat tcctttactt
    23341 tcttgtgccg tgatagctaa tgcaagatgg ccttgtttac gaaaatattc ttgccacagc
    23401 tttgtctgtt gtggatctgc cagatccgct ttattcaata aaacaaggcg aggtttttgc
    23461 tgaatgatct gatctagcat tggattacga gaagataaag gtaatcgtgc gtctaccaat
    23521 tcaaatacga tgtccacata tttcattttt tcagaaactt ctcgtcgggc ttttgccata
    23581 tgtcccggaa accattggat ggtcattgtt ctctccattc ctttcatacc ggaatattcc
    23641 ccggctttac ttgttttttt ctttttcaat ataaattcac ttttttatta taaagaaatt
    23701 ccatctatta gttaagtgaa agtgaaccag tcctcattct atcacatctt cacttttttg
    23761 atgatgcaaa tttttatttt ggtttctata tatgaatcta aatttttgac agcgtttcct
    23821 aaataagaga tgatagacga acaaaacaga aaggagacga aacaaatgaa tagtatggtt
    23881 tttgttaatt ttcctgttgc tgatgtcaat cgttccgttg ctttttatga aaagctgggt
    23941 tttaaaaaaa atgaagaatt ttctactgaa gaagctggtt caatggtatg ggatgacact
    24001 ttttggataa tgcttctctc tcatgatttt tataaaaaat ttataaaaga taaagaaata
    24061 gctgataata aaaaaacaag tggtgctttg atttcgttta gtgttgaaaa tgcggatgaa
    24121 gtaaaaaaat ttgctcaaat tgcaaaagaa aatggtggta ctttccacga agtagatatg
    24181 ggaatgcctg aagaagaaat gttttcatta gaagtccaag atctagacgg aaatacatta
    24241 gaacccgtat ggatgaagat gtaagcagat aactaatctg acgtttttta taacaaaaaa
    24301 acaaaggcgg atgatacgat aagtattctc cgcctctatt ttttttattc gtacaatctc
    24361 ttttttacag tttataaaag cagtcttctc accctttttt taattgttag atgccagtgt
    24421 atgtaaatca ccaatgtgtc atgacacatt ggtgatttta tggtatcctt ttattattaa
    24481 ctgacaaagg agagaatatt ttgaacactt tgattgcaaa agacgttttt agattgcccg
    24541 aaatgaatgc aagattgttg tcgacctctg ctcttttatt agcccttgat gtcatcttat
    24601 acaagatagc gattggtcct gcttattttc aaatcacatt cggattttta agtatggcgc
    24661 ttatgggtta tctatatgga ccgatctggg ctggtttgct tgaaatgttg tcgaacactt
    24721 taaatttcac gatatttggt tcaggaacgt ttcaagctgc ttttcttatc acggctttcc
    24781 ttggtggatt tattaatggc ttatttttat atcataaaaa gataaacctg ccttccgtca
    24841 tcgctgccca attattgatt atgatcccag tcagcctttt tatcaattct tggctgatgt
    24901 ctgttttgtt tggtgcagat ttcaaagtaa tttttctagg tagattcatc aggaatatga
    24961 tccaaatccc tattcaaata atggttgttt attcatttat caaaacttta gaggctagaa
    25021 aagtgtttaa acgagctgtt tgattttacg tactaaggta gtaaactgat tttggattaa
    25081 acaataaagc gtgcagtctc aacagactgc acgctttatt gtttctcact ggatccattt
    25141 tatttcatca agtggataat agacaaatct tgctttacct agaatatcag ttttattgat
    25201 cgtgccaaac gaacgactat cttttgaaac tcttcgatta tctccaagta caaaataatt
    25261 atcttccgga agcttggatt gtcccgttag ctcttgtagt gtaaagtccg tcgtatagga
    25321 tgcatttgca tgatctgatt tcaggttttt agttagataa ggttccttaa ctactttccc
    25381 attgatcttc aattgatcat tttggtagct gacattttct cctggaagac cgattactcg
    25441 tttgatataa atcgtgccat ctgctaattg aaaaacaaca atatcaaatc gtctgatctc
    25501 cgaaaatttt tccatcacaa ccatatcacc ttgcttcaac acgttttcca ttgaattgcc
    25561 ttccactggt accgggatga ataaaaatcc ccgcaacatg aacgcaagga atacagaaac
    25621 aagtaagtat ttgaatacaa gccagaagcg attaatatag cgttgttttt ttgtcaatct
    25681 ctatcaatcc atccctcttt ttttcattct ttctttatta taagaaaaat atcaaaaaaa
    25741 tgcgactaga tagctgaaat aaaaataatt ctttcaatta ggctattgga caaaaaactt
    25801 ttttgatcat aaagtacaaa actatctctt atatgtcatg attattttct aaatattttt
    25861 cccatgcttc gtcaaagata tccatggacg gcaaatagtc gacattcagt tccagataat
    25921 tagaaatttc ttcgtagcta gagctgtgct taggaaattg gatgtcttta ccggcttctg
    25981 tagcgaaaac agataaagca tcatgtgtgc caccgcctcg cagtgtcatt aaataatgaa
    26041 aaaagcttcg attcaccctt cgttcctcca attttcttcg ataaaggcag cccggcgtgc
    26101 atggtttctt ttaaaatttt caggatgctt tttataatac tcttgatggt cttcttctgc
    26161 tagataaaat ggttgggctg gttcgatttt tgtaacgatt ggctgttcga aacgtccact
    26221 ttcagctaac tgtttcttac ttttttcagc aatgatccgc tgttcttctg tcaaataaaa
    26281 gatcaccggg cgataattgt caccgcgatc ctcgaattgt ccgaaagcgt cagttggatc
    26341 agtttgctgc cagtaaagtt ctactaattg ttcatatgaa atcaatgttg gatcatagat
    26401 aatctccact gcttctgtat gtccagtcgt ttggcttttt acttgttcat aagtcggatc
    26461 tggtacatgc cctccagtat atcctgacat cactgtatgg ataccaggaa gcgtgtcgaa
    26521 aggctggatc atacaccaga agcagcctcc tgcaaatatt gctcgttcca ttatgattcc
    26581 ccctctaata ctactcatcc gatgattttt tattttcttt tgtcttgctt tcagtactgc
    26641 tggtgctagt tttgttggta tttgagttgt ttgaagcatc agaggtatct tttggcaaat
    26701 atagactaaa ccgaatgtca tcatttacta agtcgatttt atctgcttta atatacatcc
    26761 cgttttgcat tcggaattga tccaacctaa gcatgatagt cagatcatct gtattgactt
    26821 cgatccaatc tggtaattta tagtttctct tgatcatatt catcacatct ttgattggca
    26881 aactcaatgt tccgatagaa agacttctag cttttagttg gacatttccg ttttccatca
    26941 catacggatc aaaatataaa tagaaatcga taggaaaacc tagtacttcg aaagtaccat
    27001 tgagtagtgc ttcattttcc aaataaaatt tatattcaat atcactatct ttttgatact
    27061 cactaaggaa aaagctgatg atttgattga ctttttcttt atttgaattg atcgttaaga
    27121 caggatctcc ttgtctttcc gtaagagctg gtatttctgg gagttctggt tcccgattag
    27181 caaaaatacg gctgcctaaa aatacggtcg aaccaagtac taccccaaga aggataagga
    27241 aggcgaattt ccagtaattt gttttctttg ttttgtttga agatacgtca gatcttgttt
    27301 tcatgtttat tgatcatcct ccttctctaa ccattggttt tgtgtctcga tcagctgatc
    27361 tctcactgca tttgccatca attgataacc taaattattt ggatggaaat gatcttcttc
    27421 atagagcaca ttatttttga tgccatttag attttctgtc gtgctagttg tttgatctgt
    27481 tccttcttct gtgacaccta cttcctgatt caatccttga taaagcaaat cattgattgg
    27541 gataaagtag cagttcggca ttttttttgt caccgtttct gttcctttgt tccaattgtt
    27601 aacaattgtc tgcatgtcag tgatctcagg aaagttcaaa taaaatggat tgtagatgcc
    27661 taaaatgtaa ataggtgcgg ttttattgag ggatcgaaat tcttccaata gttctgtgac
    27721 attttcctga tagtttttca gtggcttttt aaaggaattg accgttaacc cgaataaatt
    27781 attttggata actttcatca aatcattacc gccaacagtc agtgtgataa aatcagctgt
    27841 tttgatattg tttcgaattt cttcttttgt ttttactcgc tttaagatct ggtcacttcg
    27901 ttcaccattt acaccatagt tttctgtttc aaccgcggtc aaattataac ggtcttttag
    27961 atcatttgcg acaaggggaa cgaatcctcc ttgcttggtt tgatctccta ctccttcagt
    28021 cagcgaatct ccgatagcta tgtagtgaag tttttctttt ttatttggat cttctctcac
    28081 agtagttact ggtttcatta gtgggccagc tttcggtata gaagcagcta aacctgcata
    28141 gatgatgatc gcaattaata atgggaacaa aacgaggatc aaatgtcgtt tcgttaattt
    28201 cattttttca cctcttgtat tcgattggtt ctaatgttat caaaaatttt atcaaaaagg
    28261 tagtctttat gaaaaaagaa aacaattatt ttcctaattt tataaagaaa aagagaataa
    28321 gctgcaagcc tttcaggctg agggcttatc ctcttttcga ctaatctaca taatacatga
    28381 ttgcaaacgc gtctttacca gtatgcgtag cgataaccgg attcgtatgc agtacaggga
    28441 tatgcatgtc cgggaataat tcttgcaaac cctctttgaa ttcttgcgcc aattcttttg
    28501 catccgcatg tgaaataccg atttgtcgca catttggcaa ttctttcagt tcatttttca
    28561 aattattaaa ccatttatcg aacgttttct ttcctcgccc cttagttaca gggattaatt
    28621 cagaatgggc gaaattcatc accacgcgta tgttcaaaac actagacagc aaaccagttg
    28681 ttcggctgat tcttccacct tttaccaagt tatctagtgt ggatattccg atgtataatt
    28741 ttgtattgtc ttgtactttt ttgacttctt caaggatttt ctctactgtt ttaccttctt
    28801 ttgctaattc agctgctcga attacctgaa aagataatcc ttggtcagtg gtatcactgt
    28861 cgataaccgt tactttcgtt tttgttagat tactggcttg tcttgctgct tctactgttc
    28921 cactcaagcc ttttgtcata tggatcgaaa tcacttcact gccgtcttct cctagctcat
    28981 catacatcga gatgaattct ccaattggtg gttggcttgt tttgggcaga ttttttgctt
    29041 tcgccatcat ttccataaat ctttcgccgt ctagttggtc atcatcggga tatactgttc
    29101 cgtcgatcat gatggatagc ggcatgacac gaatgcctaa agcatcgcgt acattttttt
    29161 ccatcgtaca tgaagaatct gttacgattt ttacatttgt cattattttc tctctctctt
    29221 tctttaacaa gatgccttat tcatgttaaa ctacatgtaa gacaatttct atttttagta
    29281 taacagacaa ataatgattt ttcattaatt acacctagta aaggagccgt tttcatggaa
    29341 aaaacgaaat ttaatcgaac gtatgaaata ttaaatgaag tgtttaatgc agtcacccat
    29401 ggaattggga ctggattgag tatcgcggga ctagtggttc ttttagtaaa aggtgcccac
    29461 ttgcattcct ccctccatat tgtctcttat gctatctatg gatcgatgat gattctttta
    29521 tttttaacgt ctactttgtt tcattcattg atttttacaa aagcaaaaaa ggtgttccaa
    29581 gtattcgatc atagttcgat ttttcttttg attgcaggaa gttatactcc tttttgtttg
    29641 ctgagtatcc gaggatggct aggttggact ttatttgtat taatctggtt gtttgcgatt
    29701 cttggcatta tatataaagc gttgacgttg cataaacggg agagagtatc aaaagtatct
    29761 acgatcatct atatttttat ggggtggctc tgtgtcgtgg ctgctgttcc tctttatcac
    29821 tctcttggaa tgactggtgt tattttgatg gtagcaggcg ggctttctta cacgataggc
    29881 gcattctttt atagcttgaa ttctatccgc tatatgcatg ttgtttggca tttgtttgtt
    29941 atgttaggtg caggttttat gtacttctct gttttattct ttacttaata aaaaaaataa
    30001 agctatggat gggacagctc taaataacag ctagtctatc catagctttt ttatatcgtc
    30061 aattcgcctg tcccgctttt tacaggattc tcattacgct gatacgtttc aaattgatgc
    30121 ggataacgat ttttttcatc catttctcct tgactagtat tgatcagtga ccaatcagac
    30181 cagtcaacag gtggaaaata agcatctcca tcgaatacat gatggatgac tgtacggtat
    30241 aatatcgtac aaaatggcag aaattcttga taaacagctg accctccagc gatgaaaaag
    30301 ataccgtctg tttctttttc cagagcaagc acgtcttcca cactatgcat gacatgcacg
    30361 ccttctgctt tataggaaga atctctcgtc aaaacaatcg tctgacgatt cggcaacgga
    30421 cggctaccca tcccttcgaa tgtttttcgt cccatcacta gtgtatttgc ttctgtcatt
    30481 tgtttgaaga atttcagatc atttggcaaa cgccaaggaa gacggtcttc ttttccaatc
    30541 aatccgtttt catcctgtgc ccaaattgca gctaacattt tctcatcctt tctgattcta
    30601 aacagcgata ggagctttga tcgccggatg tgggtcatat ccttctaatt ggatatcttc
    30661 catctcaaaa tcaaatactg actgcttttc ctgattcaaa actaaagtcg gaaatgcgcg
    30721 tatttctcgg ctcaattgtt ctttcatttg ttcgatatga ttgatataaa gatgagcatc
    30781 tcctaatgtg tgaacaaatt cgccaaccgc taaacctgtt tcatgtgcaa ttaggtgagt
    30841 cagcaacgca taacttgcaa tgttgaacgg aacgcctaga aacacatccc cactgcgttg
    30901 atacaattgg cagcttagcc gtccctcttg aacataaaat tgaaacatcg tatgacaagg
    30961 aggcaaagcc atgctaggaa catcttccgg attccatgca gaaacgatca atcttctaga
    31021 atcaggagtc tttttgattg cttcgatcac ttgtttgatt tgatcgataa agccaccgtc
    31081 ttttgtttcc cagtgacgcc attgatagcc gtagatgtgt ccaagatcgc cgtatgtttc
    31141 agcaaaggaa tcatcggtta ggatcttctc gcaaaaaagc ttgctctgct ttttatattc
    31201 ttcattgaat tcaggatctg tcaaacatct atggccaaaa tcagtcatat ctggtccatt
    31261 atagtctgga cttttgatgt agcgttcaaa tgcccattca tcccaaatat ggtttttatt
    31321 ttgtaataga tagcggatat tcgtatctcc ttttaaaaac caaagcaatt cgcttttgat
    31381 caatccaaaa ggtactcgtt tcgttgttaa caaaggaaac cctttttgca gatcaaaccg
    31441 catttggtat ccgaataagc ttaatgtgcc tgtgcctgtt ctgtcttctt tacgatgccc
    31501 ttcttccaac aattttcttc ctaagtcaag atacgcttgt tccatccttt ttcctcctaa
    31561 tcttctaccc attcactcaa gtattcccac cgtgccattt tttcttcaag ttgtgcttct
    31621 gtttcagata cgtcgttttg caattcttgt aatcgagtga aattatctcc ttgatgattc
    31681 atctcttctt ggagcaatga gattttttct tcaagttctg caatctcatc ttcaatcgtt
    31741 tcccattctt tttgttccat gtaagataat ttcttctttt cagcaggttc cttgctctgt
    31801 atttttggtt tcgctttttc agcaggctgc tgggtctctt tttgcttcag taaataactg
    31861 gacatgtttc cgtaataaag ttcttgtttt ccgtttcctt ggaatactaa taacttatct
    31921 gctaccttgt ccaagaaata acggtcatgg gaaacagtga tcacagcacc tggaaaactt
    31981 ctaaaataat cttccaaaat cgtcaatgtt gctatgtcca aatcattggt cggttcatcc
    32041 aataataaaa cattaggttg ctgaatcagc agtttcaaca aaaataaccg gcgtttttct
    32101 ccgcctgaaa gtttgcctat cagtgtccca tgcataaatc ttggaaataa aaatcgttca
    32161 agtaattctg ctactccgat ttgactgcca tctgctgttt ttgcttcttc agcagcttct
    32221 tgtaagtaag caatcatccg tttatctggt gccatttctt cattttcttg tgtataatac
    32281 gcaagatgaa ccgtttcacc gatagacatt gtaccgctat ccaggggaat tctgccagct
    32341 agaatattaa gcaatgtcga ttttcccgca ccatttttac cagtaatacc tagtctttcc
    32401 cgagattgaa tcaataaatc taaatgttct agcagtgttt ggtcatttat acggtaactg
    32461 ccatctttga tttccagtac ttttttccct aaacgttgtg tcgctaggtt cagctctaaa
    32521 tcatcttctt ggtttacttg gtacaagttc tcctttagat cttcgaaacg gttgatccgt
    32581 gcttgctgct tggtgccacg tgcctgtacg ccagccctca tccaagctaa ttcctgttta
    32641 taaagccgct tgcgcttttc ttcttgttct acttctacac gatcacgttc tgctttttct
    32701 aagacgtaag cttcataatt tcctttatac tcgtataatt ttccaaatga aagttcgaag
    32761 attcggtttg ctactctatc tagaaaataa cgatcgtggg taaccatcaa tagtgcgcca
    32821 cggtattgtt tcaaatagtt ttccaaccac tcgatcgctt cataatctaa atggtttgtt
    32881 ggttcgtcta gtaatagtaa atcgggttca tcaatcaaca cttgcgccag actaactcgt
    32941 tttttctgac cacctgataa agtactgatt tttttatcta attcactgat tcctaatttt
    33001 tgcaaaataa tctttgcgtt ggtatctgta gtccaagcat cttctttatt catttgttct
    33061 tctgctttag cataacgttt ttgcacatca gggtcttctc cgttttcccc tagttcgatc
    33121 aatgcttcct catatgctcg aacagtttgg atcaaggggc tgtttccttg aaagactgct
    33181 tggagaacag ttaattcatc ggaaaattca gctgcttgag aaagataacc aatacggtaa
    33241 tcagttggat aaaacactgt ttgccgatca ccgtcgctgc tatcgatacc ggcaataata
    33301 tttaagagac ttgttttacc cgtcccgttc gtcccaatca acccgatacg gtctttttcg
    33361 tggaccaaaa aagaaatctg gtcaaacaaa tctttttcac cataagtttt tttgaggtca
    33421 gttactttta attctttcaa catgttccct ccttatcttg gatattgtat cataaaaata
    33481 gtgaacttca ggaaaatttt tttggtattt gttctttttg tgttctcaaa aaaagaacag
    33541 gaccgattcg atcctgttct gatatgtttt gttttttata aaacttgaaa atttttacct
    33601 ttttatttta ttggactcgt tttttttgac tttgctcgaa acgcaaatcg gaaagaaaag
    33661 ttatcagatg gtttgttcat cgtataagat tctaacaaat tgatattcct gcgatagctg
    33721 ttcgcagaaa acgtcgtgat tttttcagca acttcgtatt catcaatgat ttttctttca
    33781 agataataac ctcttagcat ttttttcgta aacagactat cttgctggat catccacgaa
    33841 gaaatcaaat tccccgagtt catttttatt ccttcaagtt tgcgttgctg gataaaaaat
    33901 tgaaccatcg tttcatcata aatatcagtc aagttatcta aactttcaat aatcaactga
    33961 gtattttttt gatatgtctc tctgatttct tggatatctt catcactaat aaatgtctga
    34021 cgatcagcat atcttctttg ccaaaacatt ttcggatgga gaacgattcg cattaaccta
    34081 cggacaacaa tgaaccaaaa gccgataaaa gacaaaatct ggcttgtgat agaatgttcg
    34141 aatcgagaaa gaaaacggga gtaaaaacga taaccattag aagaaagctt gcccgaacga
    34201 taactttcat ccaatccatc ccgttcgatc gacaaaatca gtgcttgtat ttcttgtact
    34261 tcttgctttt catcatcggt taaatcttcc aagtacagat cacgtatccg ttcttgataa
    34321 ttttctatca ctgcttcagt ggctaaaaat tcttttgtat tacgatcaat ttcctttatt
    34381 tcttttcgca aaacttcaac gacctcttct aaaatcatca aagcattcaa atccgtgctt
    34441 ttcgcttcct ctgtttccgt cagcataggc aagacaatga tccctatgac caaagtcact
    34501 aagatgacac ccgccgtcag aaacaaaagc aaagaccgct ggtaaaacat catattattg
    34561 attgaaaatg gcaaaataaa aatcgtagcc aaactgactg ttccctttac accgccaaat
    34621 gttaagatca agatctcgtt caactgtttt ttgaactttt tactgccatc tttgaataca
    34681 taaaaaagac tgatagaaat gaatctgatc acaaaaagca tgacagagat taatacgata
    34741 atcgctaata aaagaccgtt tggatataaa ccgtctcccc atactggcga gaacacttgc
    34801 gtcaattcga ttcctaaaaa tataaatact aatgcattca gtgtaaaagc aatagttgtc
    34861 catgtactgt gggacaagct cgacaattct gcatcaaaca cagagatttt cctaaaacca
    34921 gaagcttgga gaatacctgc tgccactgca gcaatgattc cagatacatc aaatgcctct
    34981 gccaatacat aggctagaaa aggaagcaaa agttcgatca gcagataccc tgtgacatct
    35041 tgtgctgatg ctttttcgat caaatgaatt attttttgtt tgaaccagac aagcaaaaag
    35101 ccgatcaaag ctcccccaat actagaaact actagactca tccccgcgtt tactgctgaa
    35161 aaagatccgg tgatcagcgc accaagagca aactggaagg cagtaacacc agatgcatca
    35221 ttcaaaagac cttctccttc caaaatatgc atcgcctttt tagggatatt cactcgtcca
    35281 gaaagagaac tgactgctac cgcatctgtc ggacctaacg ctgctccaaa agcaaagcag
    35341 gcagcaagtg ggacacttgg caacaggaat gataacgtcg cacctacccc gacaagggtc
    35401 aaaatgactc ctccaaatgc caagaacaaa attgtcccga aatttttgag aatcgaagaa
    35461 atatctgctt tttctccttc tctaaataac aatggtgcaa taatcataac taagaacaat
    35521 tcaggttcaa aatcgattga ttgtccccac tctgtcagac ctaataccac tccaaggaaa
    35581 atttggatca gtggagctgg aatcattggc aaaatgcggc tagctatgtt cgataaggtg
    35641 atcgttactg caaatacaat aactaactca gcaaattcca tcgtccatcc cctttatttt
    35701 ttattttgtg taagcatttt taaaaattgt tcttttttct ttaaaatttt ggtttcgact
    35761 acttcaacct gtgtttggtt cccctctaat tcacactgta cacgttttga ctccaaacga
    35821 agcagatcat gtttcagacg ttccaactga cgatacatca atatatttct tttacgcaac
    35881 ttacaaagta tttgacggcg gtcatccatc gaaagggcgc catagcgatc atgcaacagt
    35941 tttttttgtt gttcaaaact ctctctcatt ttccagatct cctatacaca aacttccatc
    36001 attatagcaa agttcttttt ggatgaaaaa tatttcattg tttcatcgtt tattgctagt
    36061 tagaaatttc cataaatttt tcaacgcctc attctattcc aaatagtatc agatatcaag
    36121 taaaacttaa gaaagataaa agtttctgac aaaaggatgt ttactttccc attcctgcct
    36181 ttcttgttct tttatcacaa caaaaaaatg gggctgcgac aaaaatcttg tcacagtccc
    36241 ttattctgaa taaacggtgt ggaagagaca gcctatcagt agtaataaga cacatctctt
    36301 tcacagcctc atttttgcgt aaaaatcgat ttattcgaaa aattccccgt atacatcttc
    36361 tacacgttga tctggattga tcacgatata caagttacct gttttttcgc tgatttttgt
    36421 tgattgatat tgattgtcta atccttctgt tcctttatct agatatggga agtacacaag
    36481 atcaggtgtg ccatttcggt aaaggatttc cattcgctcg atatcttcgt atttcaatgc
    36541 gcgattaaac atgcccattt ccagtccgcc taggttgata tcatgtgtcg caactgcgtc
    36601 cccttcatgg aagatttcga ttttaaatcc agcacacgga tggatttcaa caaattcact
    36661 gccattgatt cgtccaaaac ttgttgtgat ttgtttgatc caaagatcgc cgatatgacg
    36721 gcggttgatc gtccaggttt ctccatttct aaaataaaat tttagcgctg tcatttcttt
    36781 actcattaaa aagactcctt tatgatgtaa tttattcttg tactacctca gtttagcaca
    36841 aatttatgta agtggtcaca tcttatgttt tttcttctaa tgacgtatct gcccacttta
    36901 agtagccaac aaagctgagc agatacagtc tagtgttttt taaatcaatt tttctttcga
    36961 taaaaaatcc aaaacctgtt ttttatcatt ttttattctt tgttctacga cggctttttc
    37021 agctaattgc aaagcttcac taacccactt accaggtttt tttccagttt cttctagcaa
    37081 gattttacca tctactgcta attcgcttaa tgacttgata ggtaaacgat cataggattc
    37141 tcctacggca tccatctgag gtgtttcgtc aaaatagatc atcaattctt ctacttgcag
    37201 tgcatgtttt ttgcctaaga agtacaattc gaatggttcc aaagctccag tttctcgttg
    37261 tcgtagtcct tggacaagtg cttgaacgtc tttaatcatt tggttagaac acttccacga
    37321 tttcaaaaag gaacgaatct cattttcttt tagatccagt tggctgatca ataatgccca
    37381 tgcttggctc tcttctttca atgggacgga tggtaattca gcaaaacgta aaagtgcttc
    37441 gccttcttct ttcaaaccgg gacagcaaat gtagcactct gtttcaataa atgcatggat
    37501 tcccgcggcc cgttcatttc ccaagagcat cttgatgaat tccacattta tacgttctac
    37561 agatatttta cctaatagct cgtggttttc tttgatggcg tagaaggttt cttcctctaa
    37621 tttgaaccct agttgactga caaaacgcaa cccgcgcatc attcgtaagg catcttcatg
    37681 gaaccgctca tgaggattgc ctacagctcg caagatctgt tcttctagat cttgcagacc
    37741 atcgaacaaa tcgatgattt ctccatcttc tttcaaagca aacgcattga tcgtaaaatc
    37801 tcttcttttc aaatcttctg ccaacgaacg aacgaattcg acatgatctg gtctacggta
    37861 gtcctgataa gttgattccg tccgaaatgt ggtgatctca tattgttcat cattttctaa
    37921 taccaacaca gttccatgtt cgatcccaat atctatcgtt tttggaaaaa gctgtttgat
    37981 ttcttctgga aatgcgcttg ttgcgatatc cacatcatgg attggctggt tcagcaagat
    38041 atcacgaaca cttccaccta caaaataagc ttcatatcct gcattctcta attttttcag
    38101 aactggaatt gccttttggt attccagagg taattgtttt aatttcattt ttccaacctt
    38161 tcctgcgtgt ttcttgtcct gagataaaga ctcagttgca tggtgactgt ttgacaagtc
    38221 cgtttcagag tcttcaaggc caggaccact tcatcttgtt tactcgtcct tacgctcaaa
    38281 gcgattatgg tctctttgat tgaatttttc catgtttttt tcaaaaactt cagttaagtc
    38341 gatattcaat gaattagcca tgatcatcgt aacgaataaa acatcaccaa gttcttctgc
    38401 taccgagtta actggttcag aagccttttt gcttttttct ccataagtgt ggttgacttc
    38461 tcttgccaat tcacctactt cttcagttaa tcgagccatc tgagctaatg gagagaaata
    38521 ccccaccttg aattgctgga tatattcatc cacttcctgc tgcatactgt ataaagaacg
    38581 ctttttatcc atctttttcc ctccaaaact atcttaccaa aactttaacg caaatcctag
    38641 ataagagcaa ttgagaaaat cgaatttcca tgctacattt aggaaggaat ttctaaatgg
    38701 aaattctgtg aaaatacata attgaaagct ggaattcata tggtcaaacg aaaaacagta
    38761 aaagaagttc tttttatcat tataggaaca agtatttatg catttggtct tgtttacttg
    38821 aacatcgcta atcatttagc agaaggggga gtctcaggga tcaccttgat cttaagagct
    38881 ttatttggta ttgatccagc ttatacgact ttattaatca acatcccatt gattttacta
    38941 ggaggaaaga tcctcggtaa acgttccttg gcctatactg ttctaggtac actttctttg
    39001 tcattctttt tatggatctg gcaaagggtt ccactagaaa tcaatttaca gcatgatcta
    39061 ctgatcgttt ctctacttgc gggtttagtt gcaggtgtag gaagcgggat cgtctaccgc
    39121 atgggtggaa caacaggcgg cagtgatatt atcgctcgga ttttagaaca aaattacggc
    39181 atcactatgg ggcgttccct attggcattt gatatcatcg tcttacttgc ttcattaacc
    39241 tatatcgact tgaaacgtat gatgtatacg ttgattgttt cgtatgtttt cagtcgtgtg
    39301 attgattcga tcttagatgg cggatattct gcaaaaggtc tacttgtcgt ctccaacaaa
    39361 agcgaagaga ttgcaccact actgatgacc ggtctgcaaa gaggtgtgac tttcctttct
    39421 ggagaaggcg cattctctgg taattcaaaa aaaatcatct atatggtcgt tagctctagt
    39481 gaattaactg aagctaagag gatcgtccac gagattgacg aaaaagcttt cctttcaatc
    39541 atcaatgtgc atgaagtgga agggcaaggc tttacttatt tgaagccaca gacaagatta
    39601 ctgaaaaaac tataagaaaa aagacaaaga taggctatga ggaaagattc cacatccagc
    39661 tgacctttgt cttttttagt actatactat ttttaactaa ttgttatgtt cacttacccg
    39721 gttacttttc ttttgtgtct gcaaaaggat ctttcggtta acttcatcag ataatttcaa
    39781 aaaagcatcc ctatctccat cttctagtgc ttgatcgatt tgagcataaa gcatttttag
    39841 ttttgcctct tcttcttttt cttgaaaata tcgttcaatt aattcagtat cttcttcact
    39901 gactgaagaa tcccaagaag aaaatggatt gtcttctaag atagatagat attctcttgt
    39961 ctgccaagca ttttcgaata tacattccac atataaaggc tttttccaat tcaaacggat
    40021 ttcatgaaaa atctggtctg tgtccgtaaa ttcttttccc tgcaaaaaca atttcatggg
    40081 ttccccttca acagatagat cactgatctg cagtcctcga tccgcaaaat tcgccccttc
    40141 aacaaagtgg acattgctta aaatcgcttc atgattggca agataattca agatccaaaa
    40201 aacttctctt cggctaaaag atacatgatt gaccatccac gttaaaaaat tcttcttttc
    40261 ccgtacatca attagcattt ttctcacctc tctgttaggt catctaataa tgacagtgcc
    40321 tccatatctc ctggttcaag tgttaaataa taagtcaata attcttgcgc tcttttcagc
    40381 tgcccttctt ctcttaagaa aaaggcatac tctttcaaga aatctggttc atgtgatagt
    40441 tcatgataag cctgttcata gtgtacagct gcaacggtaa agtcttctaa ttcattataa
    40501 gcatgggcta ggttccattc tgcataagga ttagcagttt cttccatctg attgatcact
    40561 tgtatcacat cttcatatct ttcatcatcc aagtaaagat tacttaaagt aagtaatgtt
    40621 tcatcttgtt tgtctcctac ttctaaagct ttcagtaaaa gttcttctgc tcgctgtcta
    40681 tcatgcagac ggaaagcatt ttccgaggcc aggtgataaa gttcgacttg gtaaggattt
    40741 tctttgattc cttcttctaa cactgtttgt gcttcttcaa tcatttcttc ttcttgaaga
    40801 gcttgtccta gatatagata taacgattga taatgcggat taacttctcg aagttcttcc
    40861 agatagttga ttgcctgctg attttctttc aattgaagat tcgtaaaagc tagctggaac
    40921 agtcgatcat ctgtcctttc ttctttcagt gcttcttcta aaggtacgat agctgcttcg
    40981 aattctcctt gcatacttaa ggattgtcct aaacgttcct gcatcgagat tcctgaaatt
    41041 tcatttatcc cgcttgccaa caatgtttca taaaccactg ctgcctcttt gaaacgatca
    41101 actgaaaaat acaattcagc tagcgcaaac tggatcaatg gttcatcagg aagcaaccca
    41161 gctgcttctt tcaattttgc ttcactcact tcagggattc cgataacttg gtacaaatca
    41221 gctatcgcca ataagctttg cggatagtaa tcactttgct tatccacttt ttccaggtat
    41281 tcaaaagcta agtcgatctc attatcttcg attgcaattt ctgctaacgg gatgttcagt
    41341 ccgtcatttt ctgggtattg gttgatcagt tgttcaaaaa tctgttttgc ttcgatcaaa
    41401 aagccaatag ccagcaattc ttcgcctagt tcagctaata tatctgctgg gtcttctttt
    41461 aatgcttttt ctaaaaagat ttgtgcttgt gacaagtctt catctgataa agcttgcaac
    41521 attttttcgc tatttgtatt catttcaatt ccctcttatc tcactgacga tttgttgaaa
    41581 taccgtcatt gttctcaata attctttttc gtttggatgt tggtatccgt ctattatacc
    41641 tattttttct aaaacaagta tttttttgct tttttctgcc aaaaaaagaa ctttttccaa
    41701 atactccatc tttgaaatct gttccagcca gtggattgga tagccaagca agtccagcca
    41761 cacaaaaaag ttttttagtt ggaaatcaaa aggatatgct tggagattcc acacgaaatg
    41821 aaacatcatt cctaaaaatt ttttcatact agcagaaagt agatggccat tttcggtcag
    41881 ataaaaagct tgttcaaaca gtttaccata atttgacagg ttttctgcct ctaattgata
    41941 aaaatagatc atttcttcga tcatcccggc aaacggccga gtcgttaatt gctttttatt
    42001 tggatagttt ttatacagat tttgcaaaaa acgatgatca caaatcattc cgcaacagat
    42061 aaaagtcaaa aagtcaatct gctttccgtc cacctgccgc tcgctgattg tttgatcata
    42121 aacgatgcgt tctggcagat tgatggtttg cagcactaca cgatttggta gctgcaaaat
    42181 cgtccgctgc ggtgttaatg ctcggcttag agaacggatc gatacaggga tcatccatag
    42241 tgatgaatca agggttgtat gtttttgaaa aaaacctgcc agatccatga tcccttcatt
    42301 tccaaatccg atgataaaca tggattgatt ttcgggatag cgtttgacga aatcaagcag
    42361 gttctttaat tctgttaaat gattgcattg agtgttggca caaatatacc agtcattgtt
    42421 tgcttgattt gcaaataacg ggttgatctt atctgcaaac aaatcataat agcgctgatt
    42481 cgttaaaaat aagattggct gatcaatgga aagttctctt agctgactga ctagcgtttc
    42541 tccgtaaaac actttcgtca acgcatcttc tacttgataa tcaagttcca tacgcatccc
    42601 tcctcttctt atttttattt taccattagt aagcaaattc tagaaaagat aagagcaaag
    42661 ctcacctcat aacttgcaaa taaataaaaa ttaaataaaa tacatgtgtt ttctatgtat
    42721 gacatacgga ttcggaatct ctgtctctta tttattcctt ttctgctagg aaataaaaag
    42781 aaaagcactt gtagccctag agccacaagc gctttttcaa ctgtcttatt taacagcgtc
    42841 ttttaatgct ttacctggtt tgaatgcagg tactttgcta gctggaattt cgatttcttc
    42901 accagtttgt gggttacgac ctttgcgagc tgcgcgttcg cgaacttcaa agttaccaaa
    42961 accgattaat tgaacttttt cgccgttagc taaagcatct tgaatagttg aaaatacagc
    43021 atctactgct gcagttgcat cttttttagt taaatcagta gctgaagcaa ctttttcgat
    43081 taattctgct ttgtttgcca tggatagatt cacctcctcc acaagagata tgatagaaat
    43141 atcatttagt taaaactttc tgagtggtcc agaagtttaa aaagaaatat tgattaactt
    43201 caatattctg ttatcacgat atcatatgaa ccgcttaata gcaaggtttt taccaatttt
    43261 cacatgcttt ttttagtcat agaaacgtaa caaacgaaac tgtctttttc ttttgaaaaa
    43321 aaaacgtcga tatggtaaaa tgcctatttt ctcctacgag ggataattcg gatggacgtc
    43381 ccttcaaaag taaatgcttt tctgatttga ttttctaaga aacgttcata agaaaaatgc
    43441 atcaattcct cttcattgac aaaaacgaca aatgtaggtg gtttgacagc gacttgtgta
    43501 gcataaaaaa tcttcaggcg ttttccttta tctgttggtg ttggattgat tgctacagca
    43561 tccataatga tatcattcaa cacagccgaa gagatacgaa gattctggtt catacttact
    43621 tgttcgatca tttcaggcag acgttccaac cgttgtttag tcactgcaga aacaaaaacg
    43681 attggagcgt aatcaagata ccggaattca tcacgaatct cttgttcgaa atcacgcatt
    43741 gtattggttt ctttctttac aagatcccat ttattcacga cgataataat tccacgacca
    43801 gcttcatggg cgtaacccgc tactcgcttg tcttgttccc gaatcccttc ttcagcattc
    43861 agtaccatca aaacgatatc agaacggtca atggcacgca ttgcacgcat cacgctgtat
    43921 ttttcagtat tttcgtatac ttttccgcgt ttacgcatac cagctgtatc tatcattaag
    43981 aatttctgac cactttcaga aacgaagtgg gtgtcgattg cgtctcgggt cgttccttca
    44041 atatccgaaa caattacacg ttcttctcct aaaatcgcat tgatcaaaga ggatttacca
    44101 acatttgggc gaccaatcaa actaaactta atcgtgtcgt cgtcctcttc ttcagtctct
    44161 tcagagaaat acttcactgc ttcatctaaa atatcaccaa tccctagtcc atggcttcct
    44221 gaaacaggat aaggatcgcc tagccctaag gaataaaatt catagatatc gtttctcatt
    44281 tctggattat ctactttatt gactgctaaa atcactggtt tattacttct gtataaaatt
    44341 ttcgcaacca tctcatctgc gtctgtcacg ccttcgcgtc cgctgactac acaaatgatt
    44401 acatctgctt cttcaatcgc gatttctgct tgatgcttga tctgatccat aaaaggttca
    44461 tcgccaagat caattccgcc tgtgtcgatc acgctgaatt ctcgacctaa ccattcacct
    44521 tttgcataaa tacgatcacg agttactcca ggagtatctt cgacaatcga gatacgctca
    44581 ccagcgatgc ggttaaaaat cgtagactta ccgacgttcg ggcggccaac gattgcaatt
    44641 gttggatttg ccatattatt tcctcctcac aagtttccaa tctcacttag tttaacgaat
    44701 gcattgccaa ctttcaagca tagattgtca attaaacgaa gtatttttat tccttattga
    44761 aatactaatc gcttttcaaa agaaaaactg agacaaaagc agctgctttc atctcagtca
    44821 cagatacaaa atcaattatt tttcagaatc atcggttgct ttttcttcta cttcttcttt
    44881 caacgcatca cctaaaatgt ctcccatagt aaatccagta ttttcttctg gtaattcata
    44941 agactcaaca ttttttggtt cttcttctga agctggtttt tcttctaaag ctttgatact
    45001 caatgcaata cgatgttctt ctggatggat ctctaatact ttcactttca cttcgtcacc
    45061 ttcatgaagc acttcatgtg gtgtcgcaat atgtttatgt gaaatttgag agatatggac
    45121 tagcccttct actccaggga acacttcaac aaaggcaccg aagcttgtca aacgtttaac
    45181 tgttccgtct aatactgtgc caactggagc ttttgtatcg atttctgacc aaggtccagg
    45241 ctgtgtgtct ttgattgaca aggaaacacg ttcttgttca ggattgatag acaacacttt
    45301 gacatttaca tcatcattga cagccaatac atcacttggt tttgctacat gactatgtga
    45361 aatctctgag acatggacta gaccgtcaat tccgcctagg tcgacaaatg cgccaaaatc
    45421 agtaagacgt gcaacacgtc cactgagaac atctccctca tggatagttg aaagaatttc
    45481 ttttttcttt tcttctcttt cgttttccac taccgcttta tgcgataaga tcaaacgatt
    45541 ttctgatggt tcgatttcga tgattttaaa gctcaatgtt ttgcctttat agtctgaaaa
    45601 gtctgcaaca aaatgatctt ccaccattga tgcagggaca aaaccacgta caccgacgtc
    45661 aacgactaaa ccgcctttga caacatttgt aacaggtgct tcgatgacct tgccctcttg
    45721 gaaatctctt tcgatatctt cccaaacttt tttagcatct aaacggcgtt ttgaaagtag
    45781 atagctgccg ttttctttat ctttgccgat cgtactgata acgactaaat ctaatacatc
    45841 accgactttt actacttcgc tgatatcttc taccggcaaa gttgataatt cttttgccgg
    45901 aacgactcct tcaactccag ccccttcgat tccgacaatc acttgtttgt cttcaatgac
    45961 taaaacttcg cccttaacaa catcgccaac ttttacttct tgtacgcttt tcattgcgtc
    46021 ttccattgac tggttgttct cttgattttg atcaaattct gtcataacac ttgtcctcct
    46081 aaccaacatt ctgccgtaag tacggtactg tttttagttt aattgattat tcaaaaaaaa
    46141 taaagcgatt tcgttcgttt tccttgaaaa aacaattgtt tttcattttt tttcttaatt
    46201 taaaaaagaa gataaccttt tttcaaaaca acagctttta ttgctgcaac gacttctgga
    46261 atcgatttgc cagtcgtatc gatacgaaca gcatcctctg cttgcttcag cggagagacg
    46321 tcccttgttg aatcataatg gtctctttcc tgtatctctt tcgtcagtgt ttcaagatca
    46381 gtcggaattc ctttttcttg gttctctttg aaacgtcttt ctgcacgctc acttacgctt
    46441 gctactaaaa agattttcac ttctgcattc ggtaatacag ctgtcccgat gtctcttccg
    46501 tccatgacaa cgccaccgcg ttcccctatt tttcgttgga cagcaactaa ttcttctctg
    46561 acttttccat gtgcagagac ttcagaaaca gcttttgtga catctggttg acggatttct
    46621 aaagtcacat cttttgatgc tatgaataca tgctgtccat cttcttgttg cgaaaaagag
    46681 ataggatact ttttgatcag cgaaactagc ccttcttcat ctgaaaaaga tacattgttc
    46741 gtaatagcta aataagttac tgcgcgatac atggcaccgg tatctgtata aatataattc
    46801 aattcttttg ctaatatctt ggcaacggta cttttcccag atgaagcagg cccgtcgatt
    46861 gcaatactga tttgtgtcat gatataactc ctttttctca gaaaaaaagc cgcaaagcgc
    46921 ggctattatt atttaattct taattcttga ccagggtaaa gcataaagtt gtttggatct
    46981 aagccattca attcaaacaa ttgatcagtg gtgatccctg cacgctcagc cacttgtttt
    47041 ggaccttcgc ctgcttgaac agtcgttgtt gaaccggctg cagctgatga agaaggtgtt
    47101 gtttctgctg cattgctgct tgaatcccct gcttgataag ttgcctcact tgaggaagtc
    47161 ggtgttgtct ctgctgcaat actgctttct gacgttacac ctgtatcttg agacacagta
    47221 gattcttccg cgattgtact agattctact gttgaagtct cagttgactc ctcttttgtt
    47281 gtacttgtag aactttctac tactgatgtc gaagtttcag aagaagttgc tgctgttgca
    47341 ttgttgcttg atccatttct gatccagaaa taagcacctg ctggaatagc gatacataag
    47401 accagtaaaa tcaaaaatag cgaaagaaat aatgtatttc ctttcttttg ttgtctctga
    47461 gaacttcttg atgaaacttc atcatctgta tcgtagattg gttgttccca tggttcttgt
    47521 gtttcgttat tttctgtaga ttgatgtcta tcttttctgc tcactgtttt acctcctaaa
    47581 ctctatcgtg tgtattatac cataggaaaa agtgtcttgt cctaaaaaat caatgttcca
    47641 tgtttttttt aaataaattt agtaaaatcg ttccccaatg aagttgttca gtcttagaaa
    47701 aaagcttcct ctttggcaga agttctgctt ttgctccatc aatatcacaa caattctgcg
    47761 gcttttcgga gagtttttcg ccaaaataag ctaagatgaa ttttctgcgg cagttttctt
    47821 cattaatata gcgaagcatc tgctgcaggc ggaattcttt ttctttttcc tgacgtttga
    47881 gccgttccac ccaattttct ggtttttcag actgctggat cagctccatc catttttttt
    47941 gcagctcatc aaagggagct tgctctgtgg catattccaa ataaagctca aaactttgtc
    48001 gctgttctct agaaagttga ttaaagaaat aatggatacg ttcatcccca ctttgataaa
    48061 aaagaattgc tgcactttct tcctgatctc tgcctgctcg accgatttct tgtacatagt
    48121 tttctagact gtctggcaaa tcataatgga ccacataacg gatatcagat ttatcgatcc
    48181 ccataccaaa tgcattcgta gcaatgagaa actgcagctg attttttaca aactgctgtt
    48241 gaagttgtct tctttgagct gcatcaagac caccgtgata gtatccaact gtaaaacgtt
    48301 ccctgaataa atgatacaat cgttcgactt ctttttttgt tgcacaataa atgattgccg
    48361 ctccttgtgc tctttccatg aagtgttcaa gttcttgttc tttttgctgc gttttccgaa
    48421 caaaaagcga gatattttgc cgattgactg attgtctgat ctcttttggt ggtcgatcaa
    48481 atagtacatg acgtatatct cttgctacta aatctgttgc tgtcgccgtt agtgccagtg
    48541 tgaccgggtt tcctaaaagc tttcggattt ttcctaactg ttgatattct ggacggaaat
    48601 caactcccca ttgagacacg caatgagcct catctactac atataacgca atattttgtt
    48661 ttttcagatg ctctaaaaga gaaggattag tcaacatttc gggactcaaa aacaaaaatt
    48721 tatattgcga tagatgacgg aggacgtatc ttctttcaga ttgcgaaagt aaactattga
    48781 aagcagctac tcgtttctcc cctcgttttt gtaattgcgt cacttgatct tccattagag
    48841 aaagcagcgg tgacacgatg atgaccagac cttccattaa ataccccgtc aattgatagc
    48901 aaaggctttt tcccgttcct gtaggcaaaa tagcgagagt atcttctcca tcaagcaacg
    48961 cctgaataat ttcttcttgg ccgggacgaa atgaatcaaa tccaaaatat ttttttaatt
    49021 catttattaa cattctgtat cgcttccttt ctcaatagat agatttgata caaacgaaat
    49081 tctccataat ctaattgtcc atctacattc cattcgctaa agcgccactc tcgcacggaa
    49141 tccttatttg gttccaaacg ttcgattgtt tctgcagaga gaatccactc gtatggaaaa
    49201 tcatcaaaaa acaaggccca ctcaataaga tgatctgtta ctgttcctct ttttaaatga
    49261 cggattgcca tgatctggtc aattgtctga ccattcataa acatctgctt ggtgatcatc
    49321 atgctttgat tgaaattttg ctgtaataat gaatcgatca aagcgtgcca aagcgaatct
    49381 ggccgttttt cgatttgtgc taacaaaagg tggatacatc tgctttgttg cagataaacc
    49441 gctgactcat cattgttttt tgccagctga taaggaagca gccctgttcg ctcatttcca
    49501 gataattggt tagctaaaaa atctgctgct tcttctggta tctccgaaaa cagctgcacc
    49561 aaactctcat acgcagtatc aatcaaaatt tcacgtggca attggctttc actaagccat
    49621 ttttttagtt ggaaagtata aaaaggactt gtctctgctg gaagatagtc ttgaataccc
    49681 gttgccagat tagaaattac ttgaacagca aatttaatga gccgccaact agtttcccct
    49741 gttctgccat agcggtcaaa acgcaaacca gtatgttcta tttgatgttc ggacaacata
    49801 tcagcccctg caggcgttag ctttgcttca ttttcattta tttcgatcca accttgctgc
    49861 gcaatttttt gcaaaatttg ataaaactta tcctgcttca attctggaaa actgttatgg
    49921 ataaacaaaa gttcgtgggt aaatccataa ataagtactg aagtcgttcg ttttcccaca
    49981 aggagttggt aaagagaact aatccgcagc ttgtgtcctg atgaaaatag cgataaaata
    50041 aaaacaatca tattattctc cttaagttag gatggttaga aatgtcacta ctatgtaaac
    50101 ttgtacctga acgctgtatt gcatgcggtc tttgtcaaat cgaagcgcca gatattttcg
    50161 attatgatga agaaggaatc gttttatttg cccaagaacc agaggctcat caacagttcg
    50221 tgatgccgga agaagaaaca tatgtaaaaa aagcgtatca aaaatgtcca gttcgagcga
    50281 ttttattaga aaataaaata taaatataaa cgaaacttta aaaacgtaca ctaagttctc
    50341 taaacttgaa aataagccgg gatctgtttc tcttgttccg tgaaatcccg acttattttg
    50401 aaagattcat ttgctatcta tagttaattg actagttttt tcacggaccc tataggaccc
    50461 atttatatta tttcaacttt ttcagccagt tttccaatgt attgacgatc acagtttgct
    50521 ggtctgcgtt agaaatttct gcctctccat ctcctttttg ctcaccatag ctgccaaaac
    50581 ctccatgatt tccgccattg attgatacat actctgttct ttccggtaaa tactgcttag
    50641 cttcttgata agcttcctga ttcaatactt tatcttttgt cgccgtgatc gataaaacag
    50701 gaacagctga ttctgtcaat gttccttttt tgtcgggata acttgccatg aagaataccc
    50761 cttttaaatg tttgtctgaa tgattcttat tgacataacg acttgccatt acgccaccta
    50821 atgaatggcc accgatcaca taatcggaat ttggaaaatc ttctttgacc ttatccgctt
    50881 tgtttccgcc catcactgct agatcaagtg gcattttgac aatagcgact gaaaagccgt
    50941 tttttgctaa atcttgcgcc caaatactat aactttctgg ctctacgaga gcaccaggat
    51001 aaaaaataag taatggagac tttccatgat tctttttctc tccttcaaaa aataagtaat
    51061 cttctttgtt ttttccttct tttaccaaca cttgcgcttc tgacgatggt tcgtacgtat
    51121 tttgctgtat atagtaataa ccggctccac cgagtaatcc taaaatgatg actaagccaa
    51181 ttagtattcg tctaaatatt ttcactttct cccacctttt ctatctgagt gacccctttt
    51241 tcttaatcaa gtatgattat accttaaaaa aataagagag aaatgcaaac tttccagcat
    51301 caaataaaaa aagacggaat atccatttga atatccgccc tttttgtatg tttattttta
    51361 tttagcacat aaacgataca cagcttctgc ataaatttcc attgctttat acatactact
    51421 taattcccat cgttcattcg cctggtgcat aaagtcagga gtatcaggga acatcgcacc
    51481 gaatgccaca cactggttca tcgtacgagc aaaagtagcg ccgcctgata caaacggttc
    51541 agtcatgtct ccagtaatat cgcgatatgt ccccaataga tttttcacta actcactgtc
    51601 tagtggcacg tataatgaat caaggaagtc aaattcttca taagtcaacc catatttttc
    51661 tgctgtttta gttaatttag ctactaaatc atctttcttg aatgtaacag ggattctcat
    51721 atctacaccg atttttgttt gttctggtgt gatttccaaa cttgcaaagt tcatcgttag
    51781 ctcgccggat tgttcatctt ctgttttgcc cacgacagct tcaccagttg cattttcttg
    51841 aactaatttt cctaagaaat tgattgggcc aaaatcaaat acttctgaaa gagcaattgc
    51901 cagacgagaa atagcattca ctccttgtgc tgcatctttc gcgtggatac ttttaccaag
    51961 aacgacgatg ccttcacctt gttcttcgaa atcgaattcg tgttttttca atgcttcttt
    52021 tacttcagaa agcttttctc cagagtaagg agctgcatca ggaacaacat tcaacgctcc
    52081 tccagctttt acagaaaact cgttcgttcc tggtcctgtc aagtaagctt gcaataagcc
    52141 tttttctgca taaattaaag gaaattcagc atctggcgca aatccttgcg taattccttc
    52201 ttctttttcg ttatattttt ctaaacaacg ccataaattt tcttcatctg taccaaagat
    52261 aaaacgaata cgtgtcttga attccacgcc agcatcaatc aaagctttga cggcatacat
    52321 tgccgcaata gaaggacctt tgtcatcttg ggatccgcga ccaactaacc agccgtcttt
    52381 tatagttgga tcgaatggtt ttgtttccca attattttcg tcccctgctg gaacaacgtc
    52441 catatgacac aagataccga ataattcatc tccagagcca atctctgaat aaccgtaata
    52501 gccttctgga tctttaaatg ttctaaatcc tagcttttca ctgatttcca atactttatc
    52561 caaagcttca atcacttttt taccgaaggg atgtccttgt cctgaatctg cttcatctag
    52621 tacggaagga attcggatca attcgctcaa tgattctaat gcttgttcat gatgttgttc
    52681 tgtcacaaat tttttcatga tattatcctc ctaatatgtt aaaaagggtc atgtcaaatt
    52741 cctccgacac aacctcttct tctcaaataa tgacgtttta agcctgctcc atcagcaagc
    52801 agaatgttct taacaaatga tgaaagaaaa aacactcact gaatggagct attgacttat
    52861 ccacgtgctt atcgatagcc gtttatcggt ttcttcattc ttatgataga ccttgtacct
    52921 gtatttgtct aggtttagaa cattgctgct acacctagga tcacacagat ggctacaaat
    52981 aatatcgcaa ttaatttacc ggtaaatttc caccacactg aaacatcgaa acgagcaatt
    53041 gctacagcac ccattacgat agcagatgtt ggtgtaatta agtttactaa accagatgca
    53101 gattgataag cagtgattac taaatcctta ccgacgccgg caaattcacc cataggtccc
    53161 atgattccca ttgttgcagc agctaagccg gaagttgaag gaatcaagaa tgacataggg
    53221 atatagaaaa taaatgtaat aatgatgaat acacttgaag ataatgaact taatccttgt
    53281 tcgccccaat gtaagatggt gtctgtgatt aatccattgt tcataaccac ttggatacca
    53341 cgagcaacag ctactacgat tgctacaccg atcaaatcag aagcaccagc taaaaattca
    53401 gagacaaatt cagactcttt catgccataa gtgattgcta ctacgatgga cattactaag
    53461 aataacatag taatttctgg gaagtaccag tcacctaatg gaagcatgct tttacctaga
    53521 accgtaccta gtaccggaag gtttgttaac cagtttgtca atgattcaaa aatcgtgaaa
    53581 ttcgcattca aggttgtcca aggaatcaaa ctgatgatca tgataccaaa agtaataaag
    53641 aacaatacat taacatgttt ttgttttttc gtgattactt cttgacgacc gattgcttca
    53701 atatcgaaat gcttcatatc ttctttacgt tggttgtata ctaatgattt tgttggatct
    53761 ttttcgatct ttgatgcata acgatacaca tatacaatag aaatagctaa caaaataacg
    53821 aacatcaaga atcgaagacc aataccttca cccatcgaaa tcccaacagc ttgtgaagct
    53881 acgcctgttg caaaaggatt taccgttgaa gctagacagc ctacttgaga ccctactaat
    53941 acgattgcta cagctactac tgtatcaaag cctacagcca tcatgactgg gatcagtaaa
    54001 gcatagaatg gaatcgtttc ttctgccatt ccgtaagtag agccgcctaa tgcaaataac
    54061 agcatcaata ctggaataag cattttttct tttcctttga atttcttcac aactgaagca
    54121 atcccggcat ctagtgcccc tgttttgttg ataataccta aaaatccacc aacaaccaaa
    54181 atgaagaaag agattgggat tgcagcaggt gtttctttag ttagctcccc cgctggtaca
    54241 cctagcatcc cattgattgg cgccatgaaa atatcccaca gcccttgagg attttgttgt
    54301 actcgttcat atgatccagc gacgatgttg cctgcatcgt ctacactata gtgcccagct
    54361 ggtataaacc aagtaagtac agcgattact gcaataataa taaataagac cgtatacgct
    54421 gaaggcattt gaaatttttt cttttttgtt tgttccatct tcttcttcct ttcttttccg
    54481 ttagagacaa caattttggc tctaatgtta ttttatcaaa aagagtatca gaacaaaaat
    54541 aataacacgc attttgtaaa acttttgttc aaaaagttcg tttttcccta ttccctcctc
    54601 ttttttcagg tttttaaagc tttcttcttt ttcattgtag cgtttccttt tttttataaa
    54661 caaacatttt cgtcttttaa aaggagaaga atttcacatt tgcaaagaat tgattgattt
    54721 ttatgcataa atcaaaaaaa tactcaaaac aaacaaataa aacgtttaca ttattgcatg
    54781 tcttgtattt ttttccaaaa aaaaagacca agaaatttct tgatcttttt gatatttatt
    54841 tgactgctgt atgacgttgt tcgaactgat gttgttttcg acttaaccat ggaagaagtt
    54901 gcgcatgcag cactaaaaag acagcactca cgatgaatcc tttgatcaag ttgaaaggaa
    54961 cgatcccaat cagtacatag tttgctaaag acatgcccag catctgtgtt gcgttcagcc
    55021 ctaaaaacat taagtaaaga ggcgtaataa cgaaataatt tgcgatactc ataaaaatag
    55081 ttagagcgat ggttcctgta atgacaccta aaaatttgtt tcttccttta tgctgtttct
    55141 ttacactaaa gaaataataa atcgggaatg taaagatcgt tgttgataag aaactagcag
    55201 tgtctcctac caaattatcc ggagaaaaac ctgtagtcag cagatgtaat actgaacgca
    55261 agaacgctgt tgcaactccg gccgctggac cgaataagaa catgctgatc aataccgaga
    55321 tatcactaaa atcgatcttc aaaaaactaa aagccggcat gatcggaaaa gcaaaatact
    55381 gtaaaactaa tgcgatcgca gcaaacatcg ctactgcaac cattttctgt acacgggtgt
    55441 tcttcatttg aaatcctcct gttaggactc ctcttcagcg aactttttga cgtttctctc
    55501 caaatgaaaa aactctattt tccagggaaa atagagttag agttatttga tcaagcgtcg
    55561 tcaaataagc tctatcttct ttatccagac tatactgtcg gtatcggaat ttcaccgatt
    55621 cagctatttt tttgcaaaat aggtcgtgga ctataaccac cggtcgggaa tttcaccctg
    55681 cccctgaaga cgaaccttat ataatttttg tttacatatt cattatacat atctattcaa
    55741 aaaacgcaac atctaacact tataaaaagt aagtgttcat tttgagtggt tcagcaaact
    55801 gctgacttct tttttgttca gttcacggta atccccagga cgtaggcctt gtaaagtcag
    55861 atctccgtat ttttcacgtt ttagtttttg cacaggaaga ccaactgctt gaagcatatt
    55921 tttcacttga tgattacgtc cttcgtggat cgtcaattcc acgatactgt ggtttgtctt
    55981 aggatccact gataaaattt ggaatttagc tggagatgtt tttcgtcctt cgatctttat
    56041 tccttttgtc aaaggtgcaa gcatcgattt tgtagcgatc ccttttacct ttgcaacgta
    56101 aactttatcc acttcatgtt ttggatgtgt caggcgctga gcaaaatctc catcatttgt
    56161 cagtagcaag atccccgaag tatcgtaatc cagtcgacct acaggataga tacgttcttt
    56221 taccatcggc aaaagatccg taacaacttt tcttcctttg tcatctgcaa cagcagaaat
    56281 aactccttta ggtttgtaca ataaatagta accgtattct tcttgataaa ttgggacttc
    56341 atccacttcc acagtgtcat gtttcccaac ttttatccct aattccctta caacttgtcc
    56401 gtttactttt acacgtcctg caaggatgaa ttcttctgct tttcgacgag aagtgatgcc
    56461 agcgtgggca attactttct gcaatctctc cattacaatt attctccttt attcattcca
    56521 tcaaagaata gatctggtga cacgtcttct tcaagtgaaa tttccatttc ttgtgtatca
    56581 ggcagttctt ctaatgattt caaaccaaaa taatctaaga agtagccagt tgttccatat
    56641 aatatcggcc gacctggtcc ttctactcta cctttttctt caatcagttt atgggcagct
    56701 agacgctgta ccgctcctga aacctgaact cctcgaatct gctcgatttc agctcttgtg
    56761 attggctgtt tataagcgat gattgctaag gtttcgacgg cggcttgaga taatgtgttt
    56821 gatgtccctt gcgcatatcg cttaagtaag ggagccagct cttttttagt cgttaataca
    56881 taatggcttt ccgtttcaaa aatatgcaaa gctgagtgcg agttttgagt gtaagattct
    56941 tttaattctt tcactaaact tgtgacttct gcttcgctga gatttaacag tccgcttaat
    57001 tcttcattac tgatcccttc ctcgccaaca ataaaaagaa gggcttcaag ttcacttaat
    57061 ttcgtcatgt tcggtatctc ctactgctgg ataaagcata attgattcat agttttcttt
    57121 ttgttgcgca atgatctgcc cttttttcat caattctagc aaagccataa aagtagtgac
    57181 aacttcactt ttagaaggtg actcaaaaaa agaatcgaaa gtacagccat gcgcatttct
    57241 ttttatacgc tcttcgataa ataccattcg ttcttcgatc gttgtgtcat cgggcgtgat
    57301 cgttttttcc actttttgag aaaatttctt acgttctaag attttctgca tggcacggat
    57361 caaatcgatt tttttgactt gtcctcgctt caacggcttt tcagcatccg ctaacccatc
    57421 gatgttcata ggttcttttg tgtaataaag acttcgttcc tctgcttttt ctgtcagtct
    57481 ttcagctgca tatttatatt tacggtattc taaaagctga gtgaccaatt cttctcgagg
    57541 atcttcttcc cataattctt catcgtctgt gacgaattct tggacaggaa gcaatgtctt
    57601 gcttttgatt gccatcaatg tagcagccat cactaagtat tctcctgcaa cagctagctc
    57661 gagtgttttc attgtatgga tataattcat atattgctcc gtgatcgtag cgatcggtat
    57721 atcatatata tcgatttcca tttgctgaat cagatgaagc agcagatcga gaggaccctc
    57781 gaatacatct aatttaatat tgatctccgt caactcattc actctttctt ctttttcttt
    57841 gctagccatt ttccaatgta aggctctgtg atttcagttg cacggatgga gacatccggg
    57901 tacttttcct gtaattcatc taacatttga aagcccatat tctcaccgcg ataagacgga
    57961 ttcaaagaaa tgtcatggat gatcagctga tcgttcgttg cccagtaaat accaataatc
    58021 ccttgaatgt tggttgaacc ttctggcgta aagaaaaaaa gttcataatc gtcttcagat
    58081 tcgtacgtat caatctcctt caacaaagaa gttttacctg tcagtttctt atggaaactc
    58141 aatagtccca tagcaatttt acggttttct ttccggtaat gtgttaacat cttctcacct
    58201 tcctatgact aagcccttgg aaaatgctgc ttataaacat ccgtcattct tttcttcgtg
    58261 atatgtgtat aaatctgagt cgtagagata tccgcgtgac ctaacaattc ctgtactgtc
    58321 cgcaaatcag ctccattttc tagcagatgt gtggcaaagc tatgccgcag cgtatgtggt
    58381 gttacttctt tgtaaatccc agcctctcgt actaactgct tcagattttt ccagattcct
    58441 tgccgagaaa gacctttccc atgattattg acaaatacat gcatttcaga tgcgtccttt
    58501 acaagtaatg gacgtgcttc gtctaagtag cgttccagcc attggatcgc ataatcacct
    58561 aaaggaatga tccgctcttt atctcctttt ccaactgtct gtaccagtcc aagagaaaga
    58621 tgaagatctc ccaatttcaa tccaatcaat tcactgacgc gcatcccagt agcatacatg
    58681 acttccaaga ttgcacgatc tcttatccct aacgtttttg tcgtatcagg tgcttcaatc
    58741 agcttttcta cttctgtcag agtcaatgta cttggtaatt tctgtgcttt cttcggcgta
    58801 tcgatatgtt gcatcggatc atgatcagtg atacgctctt gccgtaaaaa ctgatggaat
    58861 cttctgagac ttgagatcat ccgagtaaca gttgctgtcg cttttttttc ttcatgcaac
    58921 ttctccagaa aacggatgac taaaaagcga tcgacatcct gccatgaagc aattttctgc
    58981 tcctgtagaa aatgcaagta ctgttccaaa tcacgttcat agctttgtcg tgtattggcg
    59041 gacaatcccc gttcaatatt caagtagtgg agataatcgt tgatttgctc tttcataata
    59101 atccccctca tgcctctcta tcatatcaga taccgcttca ttctgatagt ctttttccat
    59161 aatctgagca aagaaaaagc cgtctagaca ctcagacgga ctggtttctt ttttcttttt
    59221 catagcattc ctgacagatt ccatggaacg ttagccgatg atcttttact aaaaaatgat
    59281 aacgttcttc tactacttgc tccacttcaa gtaaaagatc ctcttctact tcctcgatcg
    59341 tgccgcagtt caagcatagt aaatgatgat ggaaatgttt ggcgccttct tttctcaaat
    59401 cataacgggc taatccatca ttaaaactta ctttatcgac aattttcaat tctgtcaaga
    59461 tttcaagggt tcgataaaca gtagcaagtc caatttccgg actctttttt ttcactaaaa
    59521 agaagatttc ttctgcagat aaatgctctt tctcattttc caaaagcaca agcagagtag
    59581 cttctctttg cggtgtcagc ttaaaccccg cttcatgtag ctgctgtttt gtttttttca
    59641 tcgccgaaat agcattcatg accatttact caaccccaac aatttataat aattacaaat
    59701 aaaaaaagat ataacactat gttaattaaa attattataa gctagttaag aaaaacatgc
    59761 aagggattct caattagatt ttttgactag ttttgtcaca ccgttttctt gtgtaatcaa
    59821 tccttgtttc aaaagattac ctaatgcgcg cttaaatgct cctttgctga taccaaaagc
    59881 tttcatgatt tcttctggat ccgatttatc tgtaaagttg atttgctgat ctgcagaacg
    59941 ttctaaaatg gttaaaatca tttgtgcatc atctgatata gcttcatatg ctcttggctt
    60001 caatgaaaga ttcagtacac cgtctggtcg gacaccaatc actcgacctt ttatctgctc
    60061 gcctagacga ggttcattat atcgttcaga aggatggata aacccaagat agaaatcttc
    60121 cgttaaaacg tacgtacctg ttaatttcaa gcggtatgct gttcctatca catctttgtt
    60181 tttcatttct tctgttccat atttgcttaa agatttgaat attttttcat ctgctaaact
    60241 gccccaaata cggtcttttg catcaactct aagggaaatc atcaagcgat cccctttttt
    60301 aggccatagc tctgtcattg tcggcaattc atcaagagat acgacaaagt ctttatctgg
    60361 aagaccgata tcgacaaacg cacctaaatc ttttcgtgta gcagtaacga ttccaaaagc
    60421 gtaatgaccg atacggcttt taggaatttg tgtagtgatc cgattttcat gtttttgatt
    60481 ttggtaaccg aatccttcaa ctgcttcacc gattttgtgt tcaccttctg atttgcttaa
    60541 gccaaaggtt tgtccgtttt tttggacaaa gtagtttttt tcattttcat caataataat
    60601 acctgtaaat atagttgcta ataaatcgtt catttttttc tccctctata gtgaaaggtt
    60661 ttcttaatac atttctttgc tatcataaca taatgttatc tacttgaata gctgagcctt
    60721 ggtttggaaa ggtgaaaaaa ggagtatgac aacagttgtg tcatactccc ctttttcgaa
    60781 taaacggtgt taaacggcag gctcacagcc tctttcattt gcctaaaatt aattgctccc
    60841 atttcctccg aaccatccag ttttacagtt gatggttacg atgtaaaatg gcaaagcact
    60901 ttctccaatg cctttcatca atgcagattt tgttgaagtg gcatatagat tataatagcc
    60961 ttccccattt tcgattctgg ctttttctaa tctgtaacct ccttcaacga tatatccacg
    61021 ttggatacat gtagcaatca ctttgctttg gacacctggt gcccacgtcc atgctggatt
    61081 agatgcatcg gcaatatcac ttgctctggc agatttgatt ggctgattcg ctgagtttga
    61141 agaggaaggg gtcgaggatg ccgcttcttg ctgtgctgct tgtgcgaccg cagcttgttg
    61201 agcggcttga gctgcagctg ctgcttgagc ggcttgtttt gcttgttctt cagctgcttt
    61261 tttcgcttct tctgcttttt tcttttcctc ttcttctttt ttctctttat cagtgagcat
    61321 cttatctact tgtttcaatt gttcattcaa ctgagcagat agatctgtat ttttcacttt
    61381 ttctactgct tcttttgccg tcttgtattg gtcgcgtgtt gctgaatcca ctacttttcc
    61441 gtctttaaag actatagctg ttttttcttt agctgtttgc aattcagcat attgattttt
    61501 agcttctgtt tgtgcttgtt gaatcaattt tccaaaatct gtgttatccg tttcgattgc
    61561 ttggatctct ttatctgctt tcaacttgat gtcatctgct agatgatcat cgacgatggc
    61621 aggtgctatg aaccactcat tcactttttg gatatctcgg atcttagatt ggatatcttg
    61681 gtaagttgtt tctaactctg aatatccttt ttcattctta agactggata aatcattttt
    61741 tagttttgat aaatcttgat gaatcatatc tgcacgaata aattgatggt gatcatctgt
    61801 ataaaaggca gcaagtttct catgaagttc ttccatttgt tgctgtcttt ttgcttctgc
    61861 tgctacttcg gcttctactt tttgatgatg gtcataataa gcccagcttc ctccaccgag
    61921 tagaagtact gctgctaaaa cgagataagg tacttttttt gattttttct tttgcgcttt
    61981 atctggatct gttttttcta agtttgattc ttgatgtgtt ttttcagtat ctggcacgat
    62041 tgttattgat ttcgcttctg cttcaaacag cttaggctgg ttctcttctt tttctatttt
    62101 tgcttcaaga tcagtaccct ctttgcttac agattgattt tcagcatcgg attcactatt
    62161 tatttcatcg ggctctgttg actttttgtc tgtgttttcg agtatttctt ttttctctgc
    62221 ttccggcagt ttattcgttt tttccttttc taattgtttt tcagtaaata cttcagtttt
    62281 ttctgtgata tccgttttta cgtcttttat ttttactgtt tctttacctg tggcgttttc
    62341 tctatcagag tctttttcag tttcttgttc cttttctggc tctactttgt tttcctgtgc
    62401 aattttttca ctgatcattt tgagcagctc tttcgcgtgc tcttcttttg tatcttcacg
    62461 atgtgctttg atataagcgg atagtatcga attttcttcc tcattatcga cgatttcttt
    62521 cagttcagct actcttcttt tttgttttga ttcaagcgtt tcttcttgat tctttcctga
    62581 ttcttccgaa gtctcatgaa cagttgcttt cttctgttca tttttctcta tattagttac
    62641 ttgatcgtga gcaggtggtt cttcgtcttc tggttctgct ttttcgaata attccattac
    62701 agattctaga ggtaaatctt tcaattccga ccattcgatt ggatcgttta attctgtatc
    62761 aggaaagact gcatcttcag ttgatttatc ctgcatagct gcatctgtat ttgtttcttc
    62821 aattgattct tttatttgct gatcttctgc agttatttga tgaccgcagt ctggacagat
    62881 agtattctgc tccatttttt tcgatccgca atttggacat tttttcatca cttagcgatc
    62941 ggctccttta ctaggtttac gctaccattt aatctatcat attctatacg tcacgccaat
    63001 agcttatttt taggatttac ttaaaaataa tgaatgaatt tccttcctat agaaaaaaaa
    63061 gcttgagact gcgtagtctc aagctttttt tcttttaaaa taaaccaaac agttttactg
    63121 atttctcttc tggtcaatag tccacgctta ttctaagaaa tcttttaatt gttttgaacg
    63181 agaaggatgg cgcaattttc ttaatgcttt agcctcgatc tgacgaatac gttcccgagt
    63241 aacaccaaat actttcccta cttcttccaa tgttctcgtc cggccatcat ctaaaccaaa
    63301 acgtaagcga aggacattct cttcccgatc agtcaatgta tctagtacat cttccaattg
    63361 ttcttttagt aactcataag ctgcgtgttc tgctggactt gttgcttcct ggtcttctat
    63421 gaaatcacct aagtgagagt catcttcttc accgattggc gtttcaaggg aaacaggttc
    63481 ttgcgcgatt ttcaagattt ctcggacttt ttctgttgga agatccattt ccgcaccgat
    63541 ttcttctggc gtaggttctc ttccaaggtc ttgcagcaat tgacgttgga tacgtatcaa
    63601 tttattaatt gtttcaacca tatgaactgg aatacggatc gtacgtgctt ggtcagcgat
    63661 tgctcgagtg atggcttgac gaatccacca tgtagcatag gttgaaaact taaatccttt
    63721 gcggtagtca aatttttcaa ctgccttcat cagtcccatg tttccttctt ggatcaagtc
    63781 aaggaactgc atgcctcgtc ctacataacg tttagcgata cttactacta aacgaaggtt
    63841 tgcttctgcc agacgctgtt tcgcttcttg gtcgccttct tcgattttca atgctaacgc
    63901 tacttcttct tccgcagtta aaagggagac acggccaatt tctttcagat acatacgaac
    63961 cgggtcattg atttttacac cggttggcgc agaaagatct tctgtttttg cttgttcagc
    64021 ttttttctca gcactcttca agctgtgaat tgatggatca ccattttcat ccaccacact
    64081 gatacctgcg tcttcaatct tttggatcaa tttgtccatt tcatctgcat tcaaagtgaa
    64141 tggtgtagca agctgattgg ataaatcatc atatacaact tgtcgtttgg gtttgttttc
    64201 tttgatgaaa gctgctactg ctgcttcata cgttttttta tctgttccat ttgccataaa
    64261 agaaggcccc ccttgagtag ctatttggct tgttttaact gtttcgttaa attgatgatt
    64321 tcaatcgtca attcaagctc aagctgtttg tttccagttc gtctggcttc ttgctgcatt
    64381 tgttttttta atgttatttc atcagctaag ccagatttgc tgatcacatc tagcaagtct
    64441 tggatttctc gtggcgaact ttcttctgcc accggtaaat attcgatttc caccaccaac
    64501 cgccttaaat tttcatcttg caaaaagtct aaaaactctg caaggttgaa ttcataatgt
    64561 tgtgagacat atgcatcgaa taaaaaataa acttctgcat atgcatcatg aataaactga
    64621 aattctcgtt cttttaaaat attacggaca gaaacttctt taaatgcacg atataataga
    64681 atctgctctg ccttttctac ttgcgtcaaa ggtctctttt tccgtgcagt ttcttgtctc
    64741 gtaacctgag tgtgttgact ttcctgaggt tcttgacgat tttgctgatt tgtccttcga
    64801 agatcgcgca gttgtttttg tatcgtctct ctgccgatct gaaactctgt tgacagttga
    64861 gatagataca tatcttgttc gattggtgaa acaactcggc taagttcaac tagtaattct
    64921 tcgaggtatt cgatcttgtc tttttcatta tctaaatttt tcccttgttt taaataacgt
    64981 gatttaaaag taaagactgt ttcgcgccca tgagccacta attcttggaa agatgcttct
    65041 ccatacttac ggagatattc atcaggatca agcttttctg gaacgactac gacagaaagt
    65101 gctaatcgac tgttttgccg aagcaattca atccctcgat tggttgcttc aaaacctgcg
    65161 ttgtctccgt catagcaaaa gaccaattct ttcgtcagct tttccatttg ttggatctgt
    65221 tgagctgtta gacttgtccc catagaagct aatccagttt tgataccagc ctgccatgca
    65281 gcaatcacat ccataaaccc ttcgaacaac aaaatttctc cgtttttccg gatatcgctt
    65341 cttgctttat caagattaaa taaaacttgt cttttattga acagttctgt ttctggacta
    65401 ttcaagtatt tgggctgatc tttttcctgc cctttttcag gtgcaagcca gcgtcctgaa
    65461 aaaccgatcg tctggccttg agaattacga agtggaaaca tcactctttg ataaaaacga
    65521 tcagacatac ggccatcatc atgcgtgaca aacaatccag agcgttctaa caattcattt
    65581 gaaattgatt cattcttgaa tacttgctga agaaaagaac gctcattagg tgcaaaacct
    65641 atgttgaatt cttcaattaa ttctaaagac aacccacgac tttgcaaata ttctaaagct
    65701 ggctgtccag cttttgtatg caatagcatg tgatgataga tttccgctgc tttttcatga
    65761 gcagcaatca gtttccccgt agcagattca ggattttgga cagctatagt acgctgacgc
    65821 cattgatctt ctaacggtat ctgttcgaag tctgccactt ttaccactgc ttctggaaaa
    65881 cttagacctt ctagttcttg aagaaaagta aacacgtttc cgcctttacc acaaccaaag
    65941 caatgaaaaa tctgcttgtc ttctgcaaca gaaaaagacg gtgtacgttc ttcatgaaaa
    66001 ggacacagcc ccaagtaatt tttaccagac tttttcaact ggacatattg tccaatcacc
    66061 tcaacgatat tcgtttgact gcgtactgtt tcgataacct cttgaggaat ccgttgagcc
    66121 attttttcac cccctgctct attgatatct tttgggttta acagaagata tttcaggaga
    66181 ataaccggta aaaaatcgca ctaactgaaa tcagtgcgat tttaaaggac acaattatac
    66241 atgtttgatt gtatcatatt tcattgcttt ttcaagtata acacatttct atctcattaa
    66301 gcatcaattt gctactcttt tcagtaactg attttgaata tcagccttcc agtacaatac
    66361 tcgaagaaca atatttacga gcatgaatac aaagaagtat cctacgctct ctcccaaccc
    66421 taagtttact tccaaaattc catggaacag cattgcagct acatatatcc ctacgatcgt
    66481 cgttactgct aggaagatgc gcaatgggtt gaagggataa caagctttta caactgccat
    66541 gcaactgatc ccaatcagca agtaatacat caaagtagta gtttcagcat ttccccatgc
    66601 gaatgttttt ccaagtagat acaccgcaat gatattcact actacaagca aggcatttgg
    66661 taaagcatta ataagtgctg taggcaaata acggtaagtg atttttcttt tatcttgttc
    66721 aaaagataaa aagaaggaag gatacccttc aatcgctaaa tcaatcagcg tgatttggat
    66781 agggataaac ggaaatccaa ttgctgtaac ggcacatata atcgataaaa taaatgaata
    66841 aatcgttttg ataaaaaaga tgcctgagac tttcgtcaca ttgttgacca ctcggcgtcc
    66901 ttcaaacagg acttctggca atgtagtaaa atcagagtcc aacaatacca gattcgcaat
    66961 ctgacgtgtt gctccatcac cttctgccat agcaatgctg caatctgctt ctcttaacgc
    67021 caaaacatca ttcacaccgt cgcctgtcat tgcaaccgtt ctgccgcttt ctttcaattc
    67081 atttaccagc aatttctttt gctgcggact cactcgtcca aaaaccgtat agcgatgagc
    67141 agcttctcgc acttccattt cttcagtaat ttgagaaaga tcgacatacg catcataatt
    67201 cggtaatcct gcacgtctag cgatattgga gacagtaaaa ggattgtctc cagaaatcac
    67261 tttcagatcc acaccttcct catgaagata agctagtgtt tcttgagcgt tttttcggat
    67321 tggatcatcg atttcaaaca gagcaaccgg ctcaacaaat gctggttttt cttcgttcaa
    67381 ttgctgattt ggagcgatcc ccaacataag tacgcgatag ccattcgctt gggctgtttt
    67441 tagactgtcc ggaagccgat cttcttccac caaacgttca ggcgctccta cgtacacgac
    67501 tcccagttct ggaaattcca tcgcgcccca tttgcgatca gaagaaaatg cgactttgtt
    67561 tgtcacatca taccgattag aaagctcaaa ataatctctc atagcccgca tagtgatgtt
    67621 gttatcactg ctttctgcca gatacgtccc cataatttct gggatggctt tctcgtacag
    67681 cggatgtagt gcttctaccc gctggatact cattttccct tcagtaatgg tccctgtttt
    67741 atccaagcat aatgtatcga catgggccag cgtttcaatt gaatacatgt cttgaacaag
    67801 gattcgtttt tttgccagtt tggttacccc tgtcgttaat gcaatactta ttagcaatac
    67861 caaacccttt ggaagcatgc ctaacaaagc tgctgcagat gcaataacag aaattttgat
    67921 agaagcatcg cggatgaaca gtgcttccaa aaatagtatg atccccaaag gaataatcac
    67981 gaaactagtg aatttggata ctttgcgaat cgaatctaca agttcagaac gaatagcttt
    68041 gtgagtcttt gcctcgctag tcaagcggac agcataatta tctgcaccta ctcggataac
    68101 ttcagccatg acagaaccgc ttgtcaaaaa gcttccagaa agcaattcag ctccagtttc
    68161 tttgacaatc aaatcagatt cgcctgtcaa aagagcttca tttgcttctg cccgtccaga
    68221 tataacctgt aaatcggctg gtatctgttc tcctgctccg atttttacca aatctcctaa
    68281 taccagctct ttagtagaaa tcgattgttc tttaccgtcc cggatcacta gtacttctga
    68341 ttgggaaaca atcgataatt tccgtacaag atttcttgca tgtatttctt gataaattcc
    68401 aataacgata ttgacgacaa taattgctaa atacgccatg ttggaatacg cacctacaaa
    68461 taacagacaa atcccaatac caaagttcag aaaattgaat aacgtcaata tattatcgct
    68521 gaatatttgt tttgtacttt ttgatgcatc ttcctggtag tcattttgct ggccagaagc
    68581 cattttctga actacctctt gctgtgtaag tccattcatt ttctttttct ccattttccg
    68641 ccaactttca aaaataatca cgtgtttaag tctagcgtgt tccaagatta aaaacaactt
    68701 gtttttataa aatttataga aaaacacacg aatgatttta caaaaaagat gccaaccttt
    68761 ttgtttgaat cacccgtgtg ctgtttttat tatcaatcta tctttactta tattgttcct
    68821 ccaaacggct catccaccaa cctagtgctg taccggctat aaaaagaaca ggacaagcta
    68881 gtaccccagc aactcccaat cccgcgtaat cggtaggaat gatcataatc gttgaagcaa
    68941 aaactacacc taaaatgaaa tggaatagct tgctatatgc ctttgagaaa atgtaatcca
    69001 tgatttttga caatccaaga acacaaagaa tcccaccaat tccgatcggc acaatcactt
    69061 caagtcgtac ttctttaaat ccgtcagcca tcgctttata caatcccata tacaccaaaa
    69121 aattagaagg gctcaatcct gggacgatca tacccaaacc aatcaatccg ccggcaatca
    69181 gccatgttcc aaaattctga tcgacttgcc cgtcaaataa ttgagcgccg tataataaaa
    69241 acaaataagc cagaacaaaa gtaacaacca tgatgatcac atcagaagta tctcttcctt
    69301 ttttccctgc ttctttccat aacgcaggaa cagttccagc gatacatccg acaaaaaacc
    69361 ataatatcgt tgtttcatac gcacctaata aaaaactgac taaaaaagaa agtagaaaga
    69421 tgcctgtcac tccgcctaga ccaacaggca gaaaaaacaa aacattttct ttgaaatttt
    69481 ttgtaaggtg ggctaaaaaa gaaatcagcc gctcatacat gccaaagaca gctgctaatg
    69541 caccaccgct gactcctggc aagataaatc ctgaaccgat aaacattccc ttcacaaacc
    69601 gtaacatcca gtctttggtt gtgtacccca cttcttgatt ttccattttt tgcctttttt
    69661 ccactatttg cgtactcctt ctttcattta attttattca tgattgaaca tttcctgcta
    69721 ttttacttaa agactttttc ataggtctct attatttttt cattcatttt gacaatatca
    69781 ggataaaaca tgttacctcc attatgatag agatagccgc cattgtaaag caaggactgg
    69841 atccgccaat atcggtattg tttcccttcc tcatttccta aaagtgggct cagcacatct
    69901 ttagaatacg cttctgctaa ttctactgtg ttttctccgc catgattttt tacataggca
    69961 atataatctt tcccaaaatt atatgcttgg acagctgtcc ataaatcgca tccttgtttc
    70021 tttgcttcta ctatcatttc cgccaaatat ttgacacctt gatcaatact gtcagctgga
    70081 ttatcaatga tctcagcttc tccatgcaga ctttccgagc tctgcatgag atctgtccct
    70141 tttccttttg attcggtcaa tattatcgcc ttgatcaaat tctcgtagtt ttccatatga
    70201 taattttttg cagctgttgt tatttctatt tgatagcttt ccactcgttt taccgaacga
    70261 taagttcttc ctcataaaaa caacaccgca ccaataaccg tcagaatgat catgtaaaaa
    70321 agaatacgga taatagataa tcgttttcgc ttcatttcat cacctagtta ttttgacatc
    70381 tttttttatc ttactatttc tttatcttct tggctatttc ttccttgatt tctgtgtacg
    70441 ccttaagaaa aaatgaattt tcctgtattt tttgacatct tacgaaaatg atcgttgttt
    70501 ttcagtaaaa acaattgccc aatgactgat ataatcgtta tactttacac agtaggtgat
    70561 tatgtgaaaa aagaaaagaa atatgcaaaa atgccagatg ggagcgaaat ttactatgaa
    70621 aagagcggtc aaggcttccc tctcttttta ctgcatggca atgacggcag cggccgcttt
    70681 ttttcagaac aggtccctgt actcgaacgc tactataccg tgtatctagt agatagccga
    70741 ggacatggtc gttcgacaaa cgaagcatct atgttgaatt ttcagttaat ggccgaggat
    70801 ctgaatacga tcatgttgct ggaaaaaatc gatcaggcag attttcttgg atttagtgat
    70861 ggagcaaatc tagcactggt atttgccagc agcttcccta aaaaagttca tcgattgatt
    70921 ttaaattctg gaaacacgct tgttaaaggc gttcgttttt ctgccagagt catcagtaat
    70981 attcattatg catgggtatg gttactctct ttatttcgcc caagtcttcg caaaaatctt
    71041 ttagttatca aacttttgct gcatgacatc gggcttacag aaaatgattt aaaaaaaatc
    71101 aactccccca ctttgatcat cgtgggaaaa aaagatgtaa tcaagctaaa acattctttg
    71161 tatatagcta aaactatacc aaaagcttcc tttgtgcttg tgaaagaaca aggacatgaa
    71221 ttagctagaa aagatcctga acgcttcaat cgagaagttc ttcaatttct ttctgaaaca
    71281 taactattag gaatagaagg tgatttattt gttaaaaaat accatacaat ggctgaaaaa
    71341 ccacctagga ctgttcaaaa caatcttttt gatttctgtc attgtcatta ttgtcggtga
    71401 attgatgtcc atcggtaaaa ctctatctat ccagcaatta ggagaaacgt ttgccactat
    71461 acctgtatgg aaaagcggac taatgctatt gattggtctt atctctgtgt tacctatggt
    71521 tggttatgat attattttga atcggatgct tgaacaaaag cagaatcctc gttacctgtt
    71581 tgagacaagc tggctgatca atacgatcaa caatattgcc ggtttcggcg ggtttgttag
    71641 tgtggggctt cgctctgaac tatatggaca aaagaaagag agcagaaacg tgatccaagc
    71701 attatcgaaa gttttcttgt ttttgatggc tggactttct atttacagtc tgctttcttt
    71761 tttacttgta gtcttcacgc cagttgctcc ttttttgaag caatattgga tatggttgat
    71821 cggcggcggt ctatattttc ctgtcgtctt tttatttact actttcaaaa agaaaggatt
    71881 ccttggtggt acctcactta aaactcaagg acagctattg ctagtttcat tgttggaatg
    71941 gaccggtgtc ctgtttagtt tcctttctgt tggttattta atggaaatcc ggattgatct
    72001 gtggcaaaca attccattat tcattgctgc ttcagtgata ggaatcgtgt ctatgattcc
    72061 aggagaaatt gggagtttcg atgtaatgat gattatcggg ttgtccgcta ttggtgtgcc
    72121 aagagaaata gtcgttgtgt ggatcttgct ctatcgcctg ttctactaca tcattccgtt
    72181 tttgattgga atcgtctttt tcttcaaaaa tatcggttcc acttttgatc agcgttattc
    72241 tggtatccct aaacaattag caacagaaat tgcgcataaa atcgtggttg tcttattata
    72301 cttttctgga atcatgctag tactttctgc cactatcccg caagctttta cagagtttcg
    72361 ctggcttcat agtttgaacc cattgaaatt tcattttatt atccaatttc ctagtatact
    72421 attgggattt cttttaatca ttatgggcag agggatcgca gcacgtgtga aacgagcata
    72481 tttgcctaca atcttcttga ttgttctagc tttactttat gttttattaa gtgattttag
    72541 tttcacaccg gtggttttct tgtctattct gcttctgatc attcttgctt ctaaaaacga
    72601 attatttcgg gaacaattga tctattcatg ggagtggcgt acgatcgatg gaatcattat
    72661 tggtgcactg tccttgcttt acatcgttat tggggtatac aatctgccgg atttccctca
    72721 tcggcgtcac cattttattt cctttttctt atttccttct gaaaagattt ggttttcggg
    72781 attgttggcc atcatcgctg ttagtttcat gatcgttcta tttgttcatt tccttcaagg
    72841 agaaaagaaa cagattggcg aagcctttaa tgaagaaaag gccctgaaga tccttacaac
    72901 ttatggagga aactccgata gtcaattgat cttcttaaaa gataagcgga tgtttgccta
    72961 tgaaaaagac ggtgaaccaa ctgttcttct ccaattcgct tgcttcaata ataaatgtat
    73021 tgtaatgggt gatccttctg gtaagaaaga agattttcca gaagcaatcg aagcttttat
    73081 cgaagaaaca gatcgactat gctatttacc agttttctac gaaacaagcg aagagattgt
    73141 tatgattttg catgaattcg gctacgattt cattaagatg ggggaagaag cttatgtaga
    73201 cctgaatagt tttacaactt cagggaaaaa aatgaagggc actcgtgctg tcttgaatcg
    73261 gatcgaacga gaaggattta catttgatgt cttacaacca ccattttcta ctgagcaaat
    73321 gagtactttc aaaaatatct ctgataattg gttgggttca cgaaaggaaa aagggttttc
    73381 tttaggcttt ttctctgaag attatctaca aagagcgcct attgcagttg tcaaaaatac
    73441 ccaagaagaa attgtcgctt ttgcaacgat catgcctact tacacgaaca ataaagtagg
    73501 aacgattgat ctgatgcgat atgattctaa aaaagcacct tctggcagta tggatttctt
    73561 gttcttgcat ttgtttgcct atatgaaaga agaacatatc caatggttca atttagggat
    73621 ggcccctctt tcaaatgtcg gaacttccag aaagagtttt ttacaggaaa ggattgcata
    73681 cttggtttac gagtttggtt ctcattttta ttctttccat ggattaagag aatataaaag
    73741 caagtacgct acaaactggg tctcacgtta tactctatat tctagagata gttggattgc
    73801 atatgttatg attgctttgc tgattattga taatgcgcca gttgagcaga cacaaggtag
    73861 aataagtggt ttgaaaaaat ggatacgaag aaagtattaa aaactgcata ccgatagatg
    73921 aacactctca tgatgaataa aagagactga agtaaaacga aagttcatgc ttctacaatc
    73981 tcttcttata caattgaaga acaaagagcg gtccaaacag aaatcctgtt tggaccgctc
    74041 ttttatttta aacatttttc attaccaaaa tattcgagtc gcgtttctta tgcttcgatc
    74101 tctgtaacga tacctgaacc tactgtccgt ccgccttcac gaattgagaa tgtcgttccg
    74161 ttttcaacag cgattggatg gatcaagtca acatcaatcg tcacgttgtc accaggcatg
    74221 accatttctg tatcttctgg caatgtgatt gttcctgtaa catcagttgt gcggaagtag
    74281 aactgtggac gatagttgtt gaagaatgga gtatgacgtc caccttcttc ttttgtcaac
    74341 acgtacactt ctgctttgaa ttttgtatgt ggagtaatgg aacctggttt agcaagtact
    74401 tgtccacgtt cgatatcgtc acgttggatc ccccgtaaca acacgcctac gttatcccca
    74461 gcttccccgt aatctaacgt tttacggaac atttctacac ctgttacaac tgctttttgt
    74521 gtttcaggtt tgatccctac gatctctacc tcatcaccga cacgaacagc accacgatca
    74581 atacgacctg aggcaacagt tcctcgacct gtaattgaga agacgtcttc cactggcaat
    74641 agtaatggtt tatctgtatc acgttctggt gttgggatat attcatctac tgtatccatc
    74701 aattccataa tagcagcttc agcatctgga tcgccttgca atgcttttaa tgcggaacct
    74761 ttgatcacag gagtgtcatc gcctgggaaa ccatattcgc tcaataactc gcgaacttcc
    74821 atttctacca aatcgatcaa ttcttcatca tcgactaaat caactttgtt caagaaaaca
    74881 atcaggtatt ttacaccaac ttggcgtgac aataaaatgt gttcacgtgt ttgaggcatc
    74941 ggaccgtcag ttgcagaaac aactaagatt gcaccatcca tttgtgctgc ccctgtaatc
    75001 atgtttttca catagtccgc gtgtcctggt gcatcaatat gcgcgtagtg gcgtttttct
    75061 gtttcatatt ctacgtgtgc ggtattgatc gtaatcccac gttcacgttc ttctggagct
    75121 gcatcaatac tagcatagtc ttgaggattt gctaggcctt ttttgcctaa tactgtagtg
    75181 atcgcagcag ttaaagtagt tttcccatgg tcaacgtgtc caatcgtacc aatgttaacg
    75241 tgtggtttac ttctgtcata atgttctttt gccataattt acaaacctcc taaataatat
    75301 agatcactga aaaaattcag ctctcacctt tttattatag tctttttttg actaaatcaa
    75361 aaagaatacg tccgacttac tttttgtaag attcgaacta agcttattat acgtcaatat
    75421 tggtcaaagt caactaagag cactcgtc
//
LOCUS       DAZBTF010000005        66404 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000005 DAZBTF010000000
VERSION     DAZBTF010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 66404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.005"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            29..781
                     /locus_tag="ACHGNS_000375"
     CDS             29..781
                     /locus_tag="ACHGNS_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFN6936356.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            778..1692
                     /gene="pfkB"
                     /locus_tag="ACHGNS_000376"
     CDS             778..1692
                     /gene="pfkB"
                     /locus_tag="ACHGNS_000376"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFN6936357.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1710..3641
                     /locus_tag="ACHGNS_000377"
     CDS             1710..3641
                     /locus_tag="ACHGNS_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFN6936358.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(3752..5311)
                     /locus_tag="ACHGNS_000378"
     CDS             complement(3752..5311)
                     /locus_tag="ACHGNS_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFN6936359.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            5466..7700
                     /gene="pcrA"
                     /locus_tag="ACHGNS_000379"
     CDS             5466..7700
                     /gene="pcrA"
                     /locus_tag="ACHGNS_000379"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFN6936360.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            7717..9753
                     /gene="ligA"
                     /locus_tag="ACHGNS_000380"
     CDS             7717..9753
                     /gene="ligA"
                     /locus_tag="ACHGNS_000380"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFN6936361.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            9859..10164
                     /gene="gatC"
                     /locus_tag="ACHGNS_000381"
     CDS             9859..10164
                     /gene="gatC"
                     /locus_tag="ACHGNS_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFN6936362.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            10164..11636
                     /gene="gatA"
                     /locus_tag="ACHGNS_000382"
     CDS             10164..11636
                     /gene="gatA"
                     /locus_tag="ACHGNS_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFN6936363.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            11633..13063
                     /gene="gatB"
                     /locus_tag="ACHGNS_000383"
     CDS             11633..13063
                     /gene="gatB"
                     /locus_tag="ACHGNS_000383"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFN6936364.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            13073..14128
                     /locus_tag="ACHGNS_000384"
     CDS             13073..14128
                     /locus_tag="ACHGNS_000384"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFN6936365.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            14239..15615
                     /gene="rlmD"
                     /locus_tag="ACHGNS_000385"
     CDS             14239..15615
                     /gene="rlmD"
                     /locus_tag="ACHGNS_000385"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFN6936366.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIECV
                     GLLTRVER"
     gene            15824..16660
                     /locus_tag="ACHGNS_000386"
     CDS             15824..16660
                     /locus_tag="ACHGNS_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFN6936367.1"
                     /translation="MKQIFIFSDTQKDNISYFEREPAILVSMSRSDLPYLKEQDFSNY
                     PAVYVLIGGNKRYVGQAAGQSISLRLSQHFLKEYKAWVESVLFFTRADGKMSKADTDY
                     LEKRLIQDFQEKSDYEMMNSTAGNTSYIDKLQKAKSDQLYDTVFEIIDEIANIDLFGT
                     SEDSETQIYEFSSSGQYEIEYDGKKISSKSARGLLVDFVSQLFADGNYNNQLQELIVD
                     ETPSFAIILGRKPSMYNGRPNSARVADGVWVYANFNKKNVRIKIDKLARQLGIPVKIK
                     WN"
     gene            16809..17030
                     /locus_tag="ACHGNS_000387"
     CDS             16809..17030
                     /locus_tag="ACHGNS_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6936368.1"
                     /translation="MSISYNKLWKLLIDLNMNKTQLREISGVSPNVIAKLGKNELVAM
                     ESLLKIAAALNVDVSEIISSDVIDAEDVK"
     gene            17031..19748
                     /locus_tag="ACHGNS_000388"
     CDS             17031..19748
                     /locus_tag="ACHGNS_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HFN6936369.1"
                     /translation="MAKNKVLNITEIEDGVKKILSNRGDDFITQFLSLYDIPKTSITR
                     AKSKFDNGEPFVIKNKLHYEEIEGNVVIAIDAIAQSIKEQKSKPRYILVNDYLDIAAI
                     DTKTHETLNIPIEELPANADFFLAWNGIEKADYQAENPADRKAAERFAKLYDVVAKDN
                     PDANEHAFNLFLIRVLFLLFAEDTGIMNKGSFTNVLKTRTAEDGSNFNEVIKELFAIL
                     DINELARNGKDEWLLEFPYVNGKLFGEPHVDLTFSKVSRELLIEAGELLNWNEINPDI
                     LGAMIQSVASAEDRHVAGMHYTSVPNIMKVIKPLFLDDLTESFETLKLRSEENELKDI
                     TEKTRNDNKRTIINDLSALLTRISNIKFLDPASGSGNFLIIAYKEIRRLEIKILQLLG
                     DLQNNDSMPLSVIHLGNFNGIELDDFAHEVARLSLWIAEHQMNKELEEAIPGTIAELL
                     PLKDAGNIVCANSLRIDWNEVLRLKENHEIYIMGNPPYLGKSLQNAEQREDMEKLVGD
                     IRKYKLLDYIFGWFWKAKQYIATSHGSFAFVTTNSICQGEQVSILWPYLLKDEVRISF
                     AYQSFKWNNNAKNNAGVTVVIIGMTNNVNIHPKLYLENGSVMSVSNINAYLANGENVF
                     VNESRNSISNLPSLKFGSMANDGGNLVFSVAEYWNLIQKYPDIKHLFKKYLGSTGLIN
                     GDLRYTLWLTEEDATRYKQNSLIKETIKNVKSYREKSKRKETRALAEKPWSFGEVRYD
                     ETSENSILIPRVSSEKRDYVPMGIVGSDTIISDSAMAIYNAPMWLLGLLESRMHMVWL
                     RSVGGKLETRYRYSAGLVYNTFPIQDLSAQRKNEMTRVMTEILDLREYEGGTLADLYN
                     KDSMPESLRKKHQELDGIVDRAYQQRPFESDEDRLGTLLKLYQEMTIND"
     gene            19741..21696
                     /locus_tag="ACHGNS_000389"
     CDS             19741..21696
                     /locus_tag="ACHGNS_000389"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFN6936370.1"
                     /translation="MTSNLFEINFDKNQSKTTNDLGMREMQERVYEKRASQYLLVKSP
                     PASGKSRALMFVGLDKLHNQGIKKVVIAVPERSIGKSFKDTELSKFGFYWDWEVKPSD
                     NLTIGGGEKSKVQHFVKFMNSTDPSDNILIATHATLRFAFEELDDSLFDNTLLAIDEF
                     HHVSRDDKSVLGNALRSIMSNSNAHILAMTGSYFRGDSVQILEPADEDKFDKVTYTYY
                     EQLEGYKYLKSFAMGYSFYRGKYTDALDKVIDVNKKSIIHIPNVNSGESTKDKYAEVD
                     EILDLLTDGGNITQNEDGIYEIERPDGKKLLVADLVNEEGREKVSAYLASITDDIKDL
                     DKLDIIIALGMAKEGFDWPFAEYALTIGYRNSLTEVIQIIGRVTRDSPNKTHAQFTNL
                     IAQPDAQDDEVLFAVNSIMKAISASLLMEQVLAPVYKFKPKDGKPDKPGDLSIRGLKP
                     ADTTERTKKIIENDMEDLKSSILQSQDIQNAIVSGADAKMINKTLIPRVIIDRYPDLT
                     NEELETVRQHTVADINIAAGRTTTSEDGSREIIKMADRFINIDELNIDLIDQVNPFQR
                     AYEVISRDIDAPTLRFIQDYLSSNKYDFTDEQLVAAYQAAKKFRAENGRNPDRNSKDD
                     GEKYLAFALLRLAEIKREREAAKEDGE"
     gene            21686..22822
                     /locus_tag="ACHGNS_000390"
     CDS             21686..22822
                     /locus_tag="ACHGNS_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFN6936371.1"
                     /translation="MANSLDSIFEDDAFDDLTKQVEKKKVVKQDPEVAKFQEIIDFVN
                     ENGREPEKTPEWSSERALWARLSGFRGKPERSEKVKKYDTLGLLEKEFEPDGEFLADS
                     VEIDDELNLDDILGSDDMIDDFSDLLDVSRYRRTVNAADKIGRRKHASHFEKYKSLFD
                     QVHADIASGRRKIIPFEQYDIHENRFYIQNGVMMYIVSISKDTFIADNGKTNARMHVV
                     YENGTENKNLLLQSLASSLYSTERHGRMVTEPVDEETLAESFGEEFTTGYIYVLKSLS
                     TNPDISKWNNLYKIGFTRNDVESRIANAENDVTYLNAPVKVMLSAEVQNVNAQLLERT
                     LHHVFQDKQVVFQDGNFKKATEWYMVPLNDIESKINEVIAGLQK"
     gene            complement(22873..23076)
                     /locus_tag="ACHGNS_000391"
     CDS             complement(22873..23076)
                     /locus_tag="ACHGNS_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6936372.1"
                     /translation="MKFSYNPLWKLLIDRGLNKAQLRKMTDISSATLSKLNKGENVTT
                     DILLRICEALDCDLSDIVEIVRD"
     gene            complement(23092..24924)
                     /locus_tag="ACHGNS_000392"
     CDS             complement(23092..24924)
                     /locus_tag="ACHGNS_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN6936373.1"
                     /translation="MQNYYIERVTIKGKDREDATVKFTNGLNIISGPSNTGKTSIVTA
                     IDFLYGAKKDAAPFSTDATGYSKVELIIQSTNGKYSLTREFDSNSILVSKITSSGEYV
                     DYEKYNARSGKNPISHFWLQLLGIDDDYQIIKNEDFKRQKLTWRTLAPSTLIPQDRVN
                     TSASVLFPGETTQRTAFLSSLLLLLYGSDFANSEENETKEHKKIRRNSVINYINSSLS
                     ELYENYEVAKSELPEAPSDDFQQIVDGMFAELETIEKQINDALASSKILIKEIYQKEQ
                     YLHETQLLLNRYKQLHYQYQSDIERLTLIVDGERLLNDHNEHDTSHSCPFCNGELSKP
                     VQTTYIDTARSELHSLLSQLSDLTEESHDTEVELQELMEQLDELKTRKKSLDAAINDE
                     LKPKAETLKDRINKIETYRDIKQQLISMEKMQNKLKSDLEKIENEPEDERILYKPKEK
                     FDAEFYKEMSELLDSMLRNMHYKPNEFRNAFFAKDIFDIKINGNLKWTENGHGYTAFI
                     NSVVVMAYRHLLYNHAKYTPSVFIIDSPLLGLDERKKQDGDDNMKEGLFKYFMDHQNE
                     GQLIIVENTTNLPALNYDDKQVNQIVFTEDKNNGRYGYLYDIYN"
     gene            complement(24927..25373)
                     /locus_tag="ACHGNS_000393"
     CDS             complement(24927..25373)
                     /locus_tag="ACHGNS_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-three component system middle component 2"
                     /protein_id="HFN6936374.1"
                     /translation="MNSLFNTPFEISVRVMLLLSEFKEPVSADRILITDFITTYGRDF
                     EISDYNLNGDNSYRFSEFIARRELVKDAVKNLVLQHVIQPSQSKEGFKYALSEDGLKL
                     ISQFVSVYAEKYLQIADESVKYIQDKSDVELLRQINQRAKEFKGEQ"
     gene            complement(25366..26316)
                     /locus_tag="ACHGNS_000394"
     CDS             complement(25366..26316)
                     /locus_tag="ACHGNS_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-three component system protein"
                     /protein_id="HFN6936375.1"
                     /translation="MEISEITQTLYPFMGSEYVKPQDFLIALLDSVSNDDEATIFGFS
                     DDNLRRFYNGTRPLTQEYAVRVAGKFNIDKCTTFIYERLIDDAPTVLKQQLIDKGVVI
                     PKDTDVSEIVAHLLLDEITSIASQTKVKTKKAKPKLSELELASLRINTNGDFSYNDEV
                     KKFFDDLPVPLDIRAEEMTYVIALFSAYADADGYEVYEHTNDLPNSRLKDFKRQRRNY
                     YKAESIRRGVRDNFTQEEGTEHFEALKEDMYDGIEEVYEEDYDDGVERLKAVLQQSSV
                     ITLNGSILSFIPGLLQNSAKKGICHMLVNDGKIKWVTEDE"
     gene            26711..27328
                     /locus_tag="ACHGNS_000395"
     CDS             26711..27328
                     /locus_tag="ACHGNS_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936376.1"
                     /translation="MSRFMKTHQNDRSTYKYVFERTDANGRIQKEVVEIRPGEDGVTE
                     LNIQELHRADDRAIYNFYKNARPERTEEEKEKIKAWKEKFREDFNNEHGYYPMDEYVD
                     GEANDYFKRNYNLSLNEFDDECDEGDNPIQSLVYDAYQDNEEDPRIDRLREVVTQLLP
                     YHQEIYHMLVTENMSQTEVADKLDKKKQSINRTFGIIKDKIKELF"
     gene            27425..27631
                     /locus_tag="ACHGNS_000396"
     CDS             27425..27631
                     /locus_tag="ACHGNS_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936377.1"
                     /translation="MNLKHKIRVNVTDDTGNKETVLQGGVRHLPRWFARWLFGKGNMD
                     KILVLTPGDTIESVEIREVNKKGA"
     gene            27636..27950
                     /locus_tag="ACHGNS_000397"
     CDS             27636..27950
                     /locus_tag="ACHGNS_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA biogenesis protein rrp5"
                     /protein_id="HFN6936378.1"
                     /translation="MSKTKLFLQVVEDVQALADSLQELAVAMKTNEPEPQVETPEPTM
                     ETKSEVKQPTLEEVRGLLARKSQEGKSTEVKALIEKYGASRLSDVPTENYAALVADAE
                     GL"
     gene            27951..29093
                     /locus_tag="ACHGNS_000398"
     CDS             27951..29093
                     /locus_tag="ACHGNS_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HFN6936379.1"
                     /translation="MPTQHAVLSASSSNRWIHCPPSVRLSEHFENKSSPYAQQGTDAH
                     TLCEYKLNKFIGNDVTDPTSTLEFYDEEMEQCAESYATFVMEEVAKAKQTTVDSIVIV
                     EQRLDFSRYVPDGFGTGDCLVIADGTLSVIDMKYGLGILVDAYQNPQMMCYALGALEL
                     FDVIYDIQEVKMTIFQPRRENVSTYTLLKEELLQWAKDVLSPAADLAFKGEGEYQCGK
                     WCGFCPAKNSCRARADHNLKLAQYEFKPPELLSDDEIEEIIGKVDDLVSWSNDIKDCA
                     LKLALGGKQWTHHKLVEGRSTRKYSNDNDVAAAVIKAGYDPYDKKLLGVTAMTKALGK
                     EKFNEILGEYIIKPKGKLTLVDGSDKRQAVTVNNLNEEFKPLTEEQ"
     gene            29095..29637
                     /locus_tag="ACHGNS_000399"
     CDS             29095..29637
                     /locus_tag="ACHGNS_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HFN6936380.1"
                     /translation="MSKTSKVITGTNTRLSYFHGWEPASINGGAEKYSTAVLIPKSDK
                     RTIDTIEKAIDAAIEEGLAKFGGKKPNKAEIKLPLRDGDIERDDEAYQGHYFINANST
                     TAPQIVDQHVQPILDRSQVYSGCYARVSINFYAFNANGNRGVAAGLGNIQFVRDGEPL
                     GSQVSASDEFETLADDDFLA"
     gene            29698..31635
                     /locus_tag="ACHGNS_000400"
     CDS             29698..31635
                     /locus_tag="ACHGNS_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HFN6936381.1"
                     /translation="MKHLSLDLETYSSVDLSKCGVYKYSESPDFEILLFAYSVDGGPV
                     EVVDLKMDETIPAEILTSLSDETVIKHAFNASFERVCLSRFLGLDGFLNPRGWQCTMV
                     WGATLGLPLSLVGVGAVLGLDKQKLTEGKDLIRYFCVPCNPTKVNGGRTRNLPHHAPT
                     KWSAFKTYNSRDVEVEIAIQGKLSNFPVLDSVWKEYWLDQEINDRGIEIDKDFVEAAV
                     QLDELSKAGLNQQLQELTGLENPNSVLQMRGWLLDHGLETESLGKKAVAELLEQPIDE
                     NLKQVLVLRQQLAKSSVKKYQAMQNFACSDNRSRGLFQFYGANRTGRFAGRGIQLQNL
                     PQNHMSDLSEARSLVKQSNFEMLNILYGNVPNVLSELLRTAFVPREGMKFIVSDFSAI
                     EARVIAWLSGEKWRQEVFKNGGDIYCASASQMFGVPVEKHGVNGHLRQKGKIAELALG
                     YGGSVGALKAMDALEMGLTEDELQPLVNSWRDSNINITELWWAVDKAVKTCVKERRST
                     ATHGIKFEYKSGFLFIQLLSGRKLVYVKPKIGENKFGGEAVTYEGVGATKKWERLESY
                     GPKFVENIVQAIARDILCFAMENLRKYRIVGHVHDEVIIEVPMESKVEDICQLMSQTP
                     KWAEGLLLNADGYECEFYQKD"
     gene            31846..32616
                     /locus_tag="ACHGNS_000401"
     CDS             31846..32616
                     /locus_tag="ACHGNS_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HFN6936382.1"
                     /translation="MNELKVFENAEFGSVRTVMIGDVPHFVGKDVASILGYTNPQKAL
                     RDHVDEEDKTVNESFSVNGTMAILINESGLYSLIISSKMPNAKKFKRWVTNEVLPSIR
                     QHGVYATEDFITKSIEDPAWAISVLKQLQEKKEMVAMQQQMILEMKPKVSYYDLILQN
                     NSVMSISKISKDYGMSPQAMNKLLHELGIQYKQGKMWLLYQKYANQGYTQSKTHAIDA
                     ERSVMHTYWTQKGRLFLYDLLKSKKNILPVIERGDNNA"
     gene            32609..32953
                     /locus_tag="ACHGNS_000402"
     CDS             32609..32953
                     /locus_tag="ACHGNS_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFN6936383.1"
                     /translation="MPRRKDYRTSVYVCSPFSGNVVVNHKNTKRYCRIALDRGYMPVA
                     AHLMYPQFMDDATERDVAMHMSLVLMGKCEEVWVVGDTISQGMAVEIEQAEYWKKHIR
                     YFDDEMKEVSNA"
     gene            32946..35189
                     /locus_tag="ACHGNS_000403"
     CDS             32946..35189
                     /locus_tag="ACHGNS_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HFN6936384.1"
                     /translation="MLKMTLFTSNQVGNSKNGLFPTKQVVTDKETLVATVKLDHVMAE
                     YKGNYRKGDNFIQSDVIPLDCDNDHSDNPDDWVAPFEVAMQFPDVTFAVSYSRNNMKD
                     KGGKSARPRFHVYFPIPATTDKDEYVELKKQIQSEFPYFDNNAMDAARLLFGTPNSEV
                     EIYEGSKLITDFLEDDLFADWDNAQSEISEGSRNSSMSHIAGKLIKRYGATEDTYHKY
                     LEQSQKCNPPLPEEELKAIWNSAVNFGKKVKQQEGYIPPEQYNSDLSLEPTDYSDLGQ
                     ATVLAREYECKLHYSPSTGYLVYNGSYWEESKPKAQGVIHALTERQLEESETEIEKRT
                     KEMVSNGAFDVLASVGPKKAVTMFNTAQRHSFDLYQHAQEYKKFAVKRRDSKYLSSAL
                     TEAKPMLEIEQRLLDVNEFLLNTPTATFDLRTGKSQDHNSEDYITKQTECAPSDANQQ
                     IWLDALNTIFVGDQELIDYVQMIVGLAAIGKVYVEALIISYGEGRNGKSTFWNVISRV
                     LGNYSGSISADILTSQIRRNVKPELAEAKGKRLLIAAELEEGMRLNTSNIKQLCSTDE
                     IAAEKKYKDPFKYVPTHTLVLYTNHLPKVGAIDKGTWRRLIVIPFLATIDGNKDIKNY
                     ADYLFENAGGAILQWILDGAKKVIAADFHLTVPQVVANAISEYKTANDWLGHFLDECC
                     ETAVDFEEKSGELYAEYRAFCTRTGEYIRSSADFYTALEAGGFKRRRTNKGNKIVGLQ
                     LKSEFLE"
     gene            35327..35536
                     /locus_tag="ACHGNS_000404"
     CDS             35327..35536
                     /locus_tag="ACHGNS_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936385.1"
                     /translation="MKFKQWLQIGIGKRDLRFDEFYEFAMSDVTYPWKKSFDKQVAHL
                     MIHCAEKEYLEILRDAYFEYLTVWL"
     gene            35533..35817
                     /locus_tag="ACHGNS_000405"
     CDS             35533..35817
                     /locus_tag="ACHGNS_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFN6936386.1"
                     /translation="MREKQVEQALVKAVKRVGGICPKFTSPGLAGVPDRLVLMPNGKL
                     GFVEVKAPGKKPRSLQLFRMKQLTDLGFQCFVLDEIDQIPELLERIGGDV"
     gene            35817..37169
                     /locus_tag="ACHGNS_000406"
     CDS             35817..37169
                     /locus_tag="ACHGNS_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HFN6936387.1"
                     /translation="MKFVPHNYQKYAIDFIKDHKTAALLLDMGLGKTVTTLTAIKDLM
                     HDDFTIQRVLIIAPLRVTQSTWPTEIQKWDHLKDLSYSVVLGTPSQRMAALQNNANIY
                     LINRENLDWLINKSGFTFDFDMVVIDELSSFKNLKAKRFTSFMKVRHKVDRIVGLTGT
                     PSSNGLMDLFAEFKVLDMGERLGYYISRYRDKYFLPDKQNGMQIYSWKPREDAEQEIY
                     DKISDITISMKSVDFLDMPELVMNEVPVDMGLKEKQKYDEIKADLVLQLKEATIDAAN
                     TAVLSNKLLQMANGAIYDEFNVSHHIHDQKLDALEDLIEGTNGKPILIAYWFQHDLER
                     IKERFNVRQIKTSQDIIDWNEGSIPIAVIHPASAGHGLNLQAGGSTLVWFGLTWSLEL
                     YQQTNARLWRQGQSDTVVIHHIIAKDTIDEDVMLALKRKDKTQSCLIDAVKARLEGSK
                     "
     gene            37166..37594
                     /locus_tag="ACHGNS_000407"
     CDS             37166..37594
                     /locus_tag="ACHGNS_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HFN6936388.1"
                     /translation="MTPKEYLKQAYRLNKRIDDRIKEKAELYQLAVTVSSTTFEEKIS
                     SGTRNTEAPFVRNLEKCYQLEAEIDAEIDKFVDLKRQMRGVIDELTNVDEKIVLINRY
                     LLEESWDKIAEDLHADVRTVFRWHSKALENLKLPDEPIVI"
     gene            37762..38127
                     /locus_tag="ACHGNS_000408"
     CDS             37762..38127
                     /locus_tag="ACHGNS_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFN6936389.1"
                     /translation="MPMKPANPCKFPGCPNRTHDTYCDEHKTLARKRYEKYERDPAIN
                     KRYGRAWKKIRARYVAAHPLCEMCLKEGRFTSVEIVHHIKELSDGGTHDENNLMSVCK
                     SCHSKIHMTRRNTKDKTIM"
     gene            38261..38833
                     /locus_tag="ACHGNS_000409"
     CDS             38261..38833
                     /locus_tag="ACHGNS_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006702252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFN6936390.1"
                     /translation="MAKDGTQRGGRRPRSGERSQPLVDKVAAGKKASRMEFDLPDVEL
                     LIGEDIGEGVELEGNDYPEPSAYLSAKQKDGTVLGADEIYNSTFQWLKDRGCETLIAP
                     RMVESYAQNFARFVQCENALSQYGLLGKHPTTKAVMASPFVAMSQNFQKQANVTWYEI
                     QQIVKENASVEYTGDPNTDAMERLLRSKNG"
     gene            38848..39630
                     /locus_tag="ACHGNS_000410"
     CDS             38848..39630
                     /locus_tag="ACHGNS_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HFN6936391.1"
                     /translation="MIEKVNPQHPDKVADRIAGAIVDLAYAEENNPKIAVEVLIGHGI
                     CHVIIETSTALNLNEISQAINRIAGKIKKDVVIVPQDKELADNQATEIRCGDNGIFRG
                     MPLTDEQLQLSKIARDLYQDHPFDGKYILDGERLIICQSHAKTNELKQKYPNATVNPL
                     GDWTGGPDVDTGATNRKLGSDMADGVTGGGIHGKDLSKADVSVNIYAFLKAQETGMPV
                     ELSCAIGDTEIDGVPYSEIVEVARDYVNELGGFEKLAEWGLF"
     gene            39630..40865
                     /locus_tag="ACHGNS_000411"
     CDS             39630..40865
                     /locus_tag="ACHGNS_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFN6936392.1"
                     /translation="MNTTERFEKVDIDKLVPYARNARTHSKDQVKQIQASMREFGFIN
                     PVIVDKDYNLIAGHGRILAAKEEGIKQVPCVFVEHLTEAQKRAYILADNRLAMNAGWD
                     DEMLAVELSDLQGEEFDLDLLGFETDELDKLLNGNDDAQDDDFDVDEELEKPAFSKTG
                     DLWLLGRHRLVCGDSTKPETYEKLMDGKLANLVVTDPPYGVSYQGKAGTIKNDNLKDE
                     AFYNFLYDAFSCMESVMANDASIYVFHADTEGLNFRKAFVDAGFYLSGTCIWKKQSLV
                     LGRSPYQWQHEPVLYGWKKKGKHQWYTGRKESTIWEFDKPKKNGDHPTMKPIPLLAYP
                     ITNSSLSNCIVLDSFGGSGSTLIACEQTDRICHTIELDEKFCDVIVKRYIEQAGSDAD
                     VSVIRDGKTIAFNDLENPE"
     gene            40939..41316
                     /locus_tag="ACHGNS_000412"
     CDS             40939..41316
                     /locus_tag="ACHGNS_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4314 domain-containing protein"
                     /protein_id="HFN6936393.1"
                     /translation="MFPTHEQIEGMKKRYPEGSRVELIKMNDPHAPAKGTQGTVTGVD
                     DIGSILIQWDNGSTLNAVYGEDVVHIIKPDFELVFQNGRREGFETFNDAWQYVTDMIL
                     NHDLVWVDLESKTEDTIYIRKGL"
     gene            41318..41512
                     /locus_tag="ACHGNS_000413"
     CDS             41318..41512
                     /locus_tag="ACHGNS_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936394.1"
                     /translation="MKVSKRKIYNIAKNYIVQLPERGDLKAHNSDREDFLDIAVWCLE
                     DALIAAYEAGRKDGQNERKD"
     gene            41496..41672
                     /locus_tag="ACHGNS_000414"
     CDS             41496..41672
                     /locus_tag="ACHGNS_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5049 domain-containing protein"
                     /protein_id="HFN6936395.1"
                     /translation="MNAKIKQQIIEIAESGLTNMFDAIAVQRIAHDRHFYELVIFIEE
                     HKDEYSRFILEGDK"
     gene            41669..41812
                     /locus_tag="ACHGNS_000415"
     CDS             41669..41812
                     /locus_tag="ACHGNS_000415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936396.1"
                     /translation="MMDYQIYMPDNEDGIEEGYYDWQELVQLLRDNKNNPEAIQFIAD
                     MME"
     gene            41895..43460
                     /locus_tag="ACHGNS_000416"
     CDS             41895..43460
                     /locus_tag="ACHGNS_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFN6936397.1"
                     /translation="MDKDSHYDKSRADYAVAFIELLKHTKGQWFGKPFKLMPWQEKII
                     RDLFGTIKANGYRQFNTAYVEIPKKNGKSELAAAVALLLTCGDGEERAEVYGCAADRA
                     QASIVFDVAVDMIRQNPALSKRVKILTSQKRIIYQPTNSFYQVLSAEAYSKHGLNSSA
                     VVFDELHAQPNRKLFDVMTKGAGDARTQPLFFLITTAGNDTNSICYEQHQKALDIIEG
                     RKHDKTFYPLIYGIKQDDDWTDPEVWKKANPSLGITIAEEKVVAACDSAKQNPAEENS
                     FRQLRLNQWVKQAVRWMPMDKWDACSFNADEESLRGRVCYGGLDLSSTTDLTSFVLVF
                     PPEDEDDKFVILPYYWVPEDTLDLRVKRDHVNYDLWAKQGYIQTTEGNVVHYGFIEKF
                     IEELGEKYNIREIAFDRWGAVQMVQNLEGMGFTVVPFGQGFKDMSPATKELMKLTLEQ
                     KLAHGGHPVLRWNMDNIFVRTDPAGNIKADKEKSTEKIDGAIATIMALDRAIRCGNDN
                     GASVYDDRGLLFL"
     gene            43516..44250
                     /locus_tag="ACHGNS_000417"
     CDS             43516..44250
                     /locus_tag="ACHGNS_000417"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936398.1"
                     /translation="MNEYLIQVAPDKISSDPDNTLISRLFYRKWWGKILTEDLTKYAE
                     GIGNYRGETIISFNTIAGATIRLLKCYNQKNFSMPDSHYGRLKVILYSDDITEEMKVK
                     FVKFYDLSHTLANFMPLYVSKPSLNGVKGKGPYHDFPDLFYSDVRDKRYGSSTNADKF
                     IVNNKDYFSKFKDWKSFIELNYLQDFFDDEDYMTFKKLAPSSSFFPYQRKSLTPDEKS
                     KCLEEVNTFLNNAIEIILSRGSRFYI"
     gene            44316..45644
                     /locus_tag="ACHGNS_000418"
     CDS             44316..45644
                     /locus_tag="ACHGNS_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFN6936399.1"
                     /translation="MGIIKSLFKSRDKPTNQTIMNPYRFMFGGSSAGKSVTEQTAMQM
                     TAVYSCVRVLSEAVAGLPLHFYQYRDAGGKDKAMKHSLYFLLHDEPNSEMTSFVFRET
                     LMTHLLLWGNAYAQIIRNGKGEVVALYPLMPDKMTVNRDDNKEIYYLYQADVGQVRLS
                     KADVLHIPGLGFDGLVGYSPIAMAKNAIGLAIATEEYGSKFFANGANPGGVLEHPGTL
                     KDPGKLRESWNATFGGSSNAAKVAVLEEGMKYTPISMSPEQAQFLETRKFQLEEIARI
                     FRVPLHMVGDLDHATFSNVEHLSLDFVKFSLSPWLNRWEQALNKSLLSRDEKEKYFIK
                     FNVDGLLRGDYATRQQGYATGIQNGFLSPDDVRELEDLNPIPGGAGAKYYFNGNMLPI
                     EQAGAAYGVNKEESEEQDEEVLEVEESNGSESRQPRGNPRENPLPQRTNR"
     gene            45544..46299
                     /locus_tag="ACHGNS_000419"
     CDS             45544..46299
                     /locus_tag="ACHGNS_000419"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFN6936400.1"
                     /translation="MKKFWKWKNQTVLNQDNQEEILERTLYLNGPIAEESWFDDEVTP
                     QIFKDELNSGGGNVTVWINSGGGDCVAAASIYNMLADYKGKVTVKIDGIAASAASVIA
                     MAGDKVLMSPVAMVMIHNPSAVAFGEKVDMEKAIAMLDEVKASIINAYERKTNLSRVQ
                     LAHMMDAETWMDANKAVHLGFADEIIGEIDPDIPVNNSMYSEVKVTNSLTSAIAKKCR
                     IEPPEPKEREEINNTIPADSLMSRLNLMKNWRN"
     gene            46303..47514
                     /locus_tag="ACHGNS_000420"
     CDS             46303..47514
                     /locus_tag="ACHGNS_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFN6936401.1"
                     /translation="MNKIQELMEKRAQAWNGAKAFVEAKQDKDGLMSEEDAKTYAEME
                     TKVKNFTMEIERMQSMEAMDRELEKPMSQPLTSQPMTTENKMEPKKTGRASAEYKEGV
                     LEALRSNFRHVSNVLQEGVDTAGGYLVPEEYDSRLIDGMTSENIMRTLATTITTSGEH
                     KINIAGSKPAASWIEEGGELSFGDATFDQILLDAHKLHVAIKVTDELLYDNAFNLENY
                     IIDQFTKALANAEEDAFLNGDGVGKPLGIFAATGGGQVAITTNTQSAVTADDVVNLVY
                     ALKRPYRKNAKFIMNDQTISLLRKLKDNNGAYMWQPALVAGEPDRLLGYPVYTSEFVP
                     TVKAGAPVIAFGDFSYYNIGDRGVRSFDQLRELFAGNGMVGFLAKERVDGKLVLPEAV
                     QILKMKASASS"
     gene            47525..47836
                     /locus_tag="ACHGNS_000421"
     CDS             47525..47836
                     /locus_tag="ACHGNS_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFN6936402.1"
                     /translation="MSDLLNKVKANLILEHNEDDELLERLILTALSYAESYQHIESGY
                     YSEHEMPPTTEQAVIVLASNFYESRDGSTGGFFADNVNASKQVWETVNLLLRLDRRWQ
                     V"
     gene            47833..48171
                     /locus_tag="ACHGNS_000422"
     CDS             47833..48171
                     /locus_tag="ACHGNS_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFN6936403.1"
                     /translation="MSFGKMNQRIQIVKVTNQKDSAGFSVKQDEVLAAVHAYKEEKNA
                     TEKWVNMATFSSATCLFRIRYIPNLEVTTEMVVISDQKRYQIVSVENVRNRNMYLEML
                     TTEMEVSADG"
     gene            48164..48595
                     /locus_tag="ACHGNS_000423"
     CDS             48164..48595
                     /locus_tag="ACHGNS_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFN6936404.1"
                     /translation="MAKALMKMPEDFLLKVSKLENKTDEIIPKVLESGAEVVEGKVRS
                     NLTAVVGSNTKVPSRSTGELESALGITQARQDKSGNWNIKVGFDEPRIDGSSNAKIAN
                     ILEYGKHGQPAKPFLKPAKSQSRKSCIETMKSKMESEVNGI"
     gene            48592..48933
                     /locus_tag="ACHGNS_000424"
     CDS             48592..48933
                     /locus_tag="ACHGNS_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936405.1"
                     /translation="MNILEELNSLFATLHIPVETGEFSDKAPDKYCVLTPLADRFDVY
                     GDNFPTVDVQEVRISLFVKGNYLNTQKQLVQNLLKHEFTITERLFVGFEKDTNYYHVA
                     IDVAKHYEMEV"
     gene            48936..49526
                     /locus_tag="ACHGNS_000425"
     CDS             48936..49526
                     /locus_tag="ACHGNS_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFN6936406.1"
                     /translation="MATIGLDKLYYAPITESATTGEETYGTPAMLAKAISAELSVELA
                     EATLYADDGASEVVKEFKNGKLTLGVDDIGRAVAAVLVGATVDQNGVLISTTEDGGTP
                     VAIGFRARKSNGKYKYLWLYRVKFAVPSTSLATKGDDITFSTPTIEGTVMRRNKLDAS
                     GKHPWKAEVDENDTGVEQTTINGWYTSVYEPDFTAD"
     gene            49536..49916
                     /locus_tag="ACHGNS_000426"
     CDS             49536..49916
                     /locus_tag="ACHGNS_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936407.1"
                     /translation="MSEERSAKISLGGDEYELILTTKATKEIASHYGGLDNLGEKLLK
                     SENFELALDEIIWLITLLANQSIKIYNLKNTEKKDELTTEYVELMTSPLQLAEYKSAI
                     TEAMFKGTARNIESETDSKNKAGE"
     gene            49964..50104
                     /locus_tag="ACHGNS_000427"
     CDS             49964..50104
                     /locus_tag="ACHGNS_000427"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936408.1"
                     /translation="MGMTSDDFWYCSLGLFLDLWECHKQFTGISKPKVELFIDDVIPS
                     GI"
     gene            50624..52684
                     /locus_tag="ACHGNS_000428"
     CDS             50624..52684
                     /locus_tag="ACHGNS_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFN6936409.1"
                     /translation="MGTVALAAGAAAVELGKQVVSAYADYEQLVGGVDTLFKDASSTV
                     QKNADNAFKTAGMSANEYMETVTGFSAALISSLGGDTKKAAGVADQAVTDMSDNANKM
                     GTDIESIQNAYQGFAKQNNGMLDNLKLGFGGTKSEMERLLQEAEKISGVKYDISNFAD
                     VTEAIHVIQTEMGITGTTAKEASETISGSIDGMKSAISNLMAGLGNSSADIGMLIGNV
                     VEQFQNVIKNILPVIQNIVSALPAVVDAIIQALGQLLPTLLEAVTSLFSQVLETILGL
                     LPTLIPVVVQAVLTIVQTLIENLPLLIDAAFQLVTTLIQGLGEALPELIPITVQAIIT
                     IVNGLIENLPLLLDAALQLIMGLAQGLITALPILIESLPQIITGIVNFIISAIPQIIQ
                     TGIQLLTSLVTALPDIINAIVAAIPVIIQNIINAVIGAIPQLIDAGIQLLVALIGALP
                     QIITTIANAMPQIINGITGTLVNNIDKIINAGVKLLVALVQNLPAIISAIVKAVPQII
                     TGLANAFGEYVSTMGTIGKNLITGLWNGIADAGSWLRNKISGFFGGVVDNIKDFFGIH
                     SPSTLFRDEIGKYMAQGIGVGFDKEMTGVSKTMQKAIPTKLYMPSFDIDTDIHAAVDS
                     TFSMLDLGAKVDALTNITSEMLPTLLKAMDIKVVLNDGTLVGKLAPEIDKSLATLNRR
                     RVAY"
     gene            52755..53156
                     /locus_tag="ACHGNS_000429"
     CDS             52755..53156
                     /locus_tag="ACHGNS_000429"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936410.1"
                     /translation="MLKHKFKAKKPALIGYILLLAIGLFLTAVRWYSGINHGFVFINN
                     QINSHISNFALSFLFYLVIGYLWLLMGTNLRVLTAMGAGLIVANIVCETMMTFLNTPD
                     LIDAIYGISGVLVVYAYLAITKKIGLEETTE"
     gene            53233..53925
                     /locus_tag="ACHGNS_000430"
     CDS             53233..53925
                     /locus_tag="ACHGNS_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFN6936411.1"
                     /translation="MNAFILDGTINSRTDLGLRITEVPVIPVTERVIDSIEIDGREGD
                     LTILKGWKDVTFGFKVVIWKSDVWTSWQIILPQILNAKTIAFSNEPNVFYPIKYAKAS
                     GLTQILSSMWEFEMELTCSPFRYKTNVATINRTTSGTVNNPGNIYSLPKIKVYGNGTR
                     TLTINGKPIVLNLTNGNLTLDSDLKECFYGDVAANQYMTGDFPEFRVGSNTVTLGTGI
                     TKVEIEPRWRYL"
     gene            53922..57812
                     /locus_tag="ACHGNS_000431"
     CDS             53922..57812
                     /locus_tag="ACHGNS_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFN6936412.1"
                     /translation="MITLYNKTESNFNHNGLAVLDGNILNPVVSEELNGLFSFEFDYP
                     IHAPHSEELLPEMIVKAPVPELSDQLFRIVERDDALGGFLHIVAHHIFYDLATNFIED
                     TYIVNKNGSGALTQLLGATQFMHRFTGTSNISAVNNVRLVRLNPVEVLLDSDLENGFQ
                     SSYGGEIVRDNFTISMLTTRGSNNGVQIRDKKNLTGYKADVDYSGIVTRIMPQGYDGL
                     FLPEKYVDSPNINQYVQPKIKVIKYDNVKVGDEEDEFTQAEAYDELRRLAGLEYSANK
                     VDLPKATYDVEFAPLERTEEYKDFAQLETINLGDTVSVIHAEDGLNITARMVSYKYDP
                     FLKAYISVTLGNITPKFTDVAKDINRVDNKAEQAKDDANYALSSANGKNTNFYGPTTP
                     NNPKLGDVWYKENGDKFEMWVYEIRDGVTQWYPLMTDMTAEEVKQAVEQAQKDANTAT
                     EKANQAFNDAVNALNAAESVGASVETLTTSVEQNTSKISLVASGMSTLEGRVITAEST
                     LTVQAGQIASKASQSTVDNLTGRITTAESTITQNADRFELSLSKTNKAVNVLAGMSIA
                     DLQWAVGGLNTNDGKENTYGGYVRSGFTRVRNGEKYLLQTFVGGKLYSTYATAYLLYY
                     KEDKSFLSYTQFGNTTTPLTVPSSAAFIRIRLTTSDAPETISCYMLKTDVTNGYVDLS
                     EISNVVKLTATTDALLLSVSETKEAIGVPFKVNQWEQGSLSTTDGSEVSATNYLRSEF
                     LDVKVGDKYIGQLRDGTQAAFYYHYYGMDLPYIDFVPTAKQFSETLTAGTYTNATIAS
                     YLVSKNVESMSITSGVTTNPYSNSGDYLVIYKLPLEGKVAPSVISWNGRKDTSDNIVL
                     LYTGGAWEQISTVTSSSNATYSWTLTAEQISGITTDSVYLAFFSVKTGTYAGIYNSSN
                     TPFTLNPSDGSSYHQISYTGSSGAITVPSGTLKMRVRVNTSIKPDEYEGNIYPTTTRQ
                     DYTNANTLYSAILMQKDLINLRVGKGDVINQINISPESILIAGNKVHITGQTTIDSAV
                     ISSAMIQNAAITNAKIADATIQSAKIASIDAAKITTGTLSAARIGANSITADKIAANI
                     LTALTASSSIRITGTTIGYYSGSTLVTEINSQGMTIRRGGTTVGTIGANNISGHSDWR
                     GLVFDLEYGTEYMSWAHKDSSSASVYTQKLSWYARTLQSGMEKGFHFDDDIYLPSMLR
                     IGTQHQISVGGITFSSSLYTKIGSSSGSAGIAFNGSNLMLGDDGKWVDFGVIREICAK
                     LAGRTIALPTGFSSNGTASGWYNPQSFNSMTTYS"
     gene            57822..57989
                     /locus_tag="ACHGNS_000432"
     CDS             57822..57989
                     /locus_tag="ACHGNS_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936413.1"
                     /translation="MNEEKEEVIVTEPIEADIPKPMKVGTDMVDLLVMVVDDLCVQIG
                     DLKEKIDNAQN"
     gene            57976..58383
                     /locus_tag="ACHGNS_000433"
     CDS             57976..58383
                     /locus_tag="ACHGNS_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1617 family protein"
                     /protein_id="HFN6936414.1"
                     /translation="MLKIRNEDLVDVMSFLKGFDLPPKISRVRTKLAKLIQVKVDELY
                     QDEVDLLQKYGKKDEHGNLVQHDGAFSLIPETATEYHKEKADLLNEETHINVDELQDK
                     LPLLVAGFENSEVTISGKDAEMLDLILEQLEDL"
     gene            58384..58803
                     /locus_tag="ACHGNS_000434"
     CDS             58384..58803
                     /locus_tag="ACHGNS_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin family protein"
                     /protein_id="HFN6936415.1"
                     /translation="MKEVWNWIQLAIAGGGGVIGWYVGGIDGLIYTLVTFVVVDYVTG
                     LLRAINEKKLSSKIGAKGITKKIMIFLLVGVAHMLDVEVIKGGNVLRDAVIFFYISNE
                     GISLLENAVAIGLPVPEKLREILQQLHEKKEETENGN"
     gene            58793..59701
                     /locus_tag="ACHGNS_000435"
     CDS             58793..59701
                     /locus_tag="ACHGNS_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFN6936416.1"
                     /translation="MGISNLATKYGFQSFSHYSNGRSGAKVDKIVIHHMATTNYDIVP
                     DIWKTREASAHYGIGKNGEIRAYVDEGNTAWHAGNWNANISSIGIEHCNSTGSPNWGI
                     NQATIDASAKLCADIAKRYGLGKLVVNKNIFPHSHFSATSCPGQLLGKLQEIADKANA
                     INNGSSTPSTPASGLYRVRKSWADAKSQIGAYKDLNNAKRAADSHSGYKVFDQNGNQV
                     YPSGSSTSNVKTLVVQVSGLNVRNKPSLSGAVVGSYNKGATWTLPKSEKMTIADGWVW
                     ARCPIGYCAVGKNTGKTESDDYILIS"
     gene            59817..60854
                     /locus_tag="ACHGNS_000436"
     CDS             59817..60854
                     /locus_tag="ACHGNS_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014572406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HFN6936417.1"
                     /translation="MVTFKDLLPEPTESQQAFILDHGKTEDDGQLKYADDAKSYGWNM
                     RQYGKLKAGAVVLNRHPGKITKDRKWEIYGGGYVESISEPDEAGNVTAVITHAFTIEP
                     AIKQGDSFIENFDWNTPSKKKRKKPNSWAYFWDQYGMNEITYADFVALIENRHLIPID
                     DTQSLPSELDLTNEEVEEIEQESSKGFTVLVDEVGPNRTNGTQKRKFTGRHTDWERVN
                     KAKQKTGALGEEIVLDFLIQEAEKNKTKLPEHVSKTEGDGHGYDICAFDQSGNEIHIE
                     VKASKTNFSDGFEMSTNEVAASLGDTPYKIYFVHDLDVASKVCKIKIYDGPITEENFT
                     MVPTSYKIFNK"
     gene            complement(60915..61220)
                     /locus_tag="ACHGNS_000437"
     CDS             complement(60915..61220)
                     /locus_tag="ACHGNS_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019299341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936418.1"
                     /translation="MSSFDMEGLEKEYEHFCKSATTKELEEFDESFKQEFEKCFKTIE
                     AEEALYQFLQANINAMNNRMESIESPLFCINYKEQVFQLRNHLILMQNAVNTYLKEN"
     gene            61515..61688
                     /locus_tag="ACHGNS_000438"
     CDS             61515..61688
                     /locus_tag="ACHGNS_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HFN6936419.1"
                     /translation="MTNKKTEINYSDEAAYYISQQILLNLLVTGKINLEEYVKIDNKN
                     RETFNPFLSRIMV"
     gene            61749..63302
                     /locus_tag="ACHGNS_000439"
     CDS             61749..63302
                     /locus_tag="ACHGNS_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN6936420.1"
                     /translation="MKTIHKLARPELPEASKLKVAAYARVSTSSNEQLASLQTQITHY
                     ENHIKNHEQWDYVGVYYDEGISGTKTVKRDGLKRLIKDAELGKIDLILTKSISRFSRN
                     TVDCLNLVRQLTDIGVTIFFEKENINTGDMESELLLSILSSLAESESYSHSENLKWAI
                     RNRMSKGKFKAYPPYGYKRKGYDFHIVAAEAKVVKQMFQWALDGISTGMIAKRLNDGK
                     QFTKRGANWQATSVRSILKNEVYTGTLTMQQTFVDDQYKRRINKGELPMYQIKNNHEP
                     IVSKEDYDRVQELIRRKAEAKGNTGDGKKYTKRYAFSGNIICDKCGSKFKRVHTYWKG
                     NTKKVQWKCTGHLKNKDSCDALPITDEDLKVAYLTMLNKLIFGHKLVLEPFMKSLNKA
                     GANQEGLEVLADEIDKIQEKLEVLASLTASSILSTKVSLEEQSKLQQERAVAQEKQRL
                     IIESVNGKSTQQIELEALYKFARMAEMLTDWDEDLFKKFTNSITVYNRQEVGFELKCG
                     LLLKERLPE"
     gene            63350..63715
                     /locus_tag="ACHGNS_000440"
     CDS             63350..63715
                     /locus_tag="ACHGNS_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN6936421.1"
                     /translation="MDEQQAQQIREFFNIYLEGVGLQEAGKRSGINKPHPFMKRILMN
                     EVYLGNEIYPKIIDEDIFFQADLEQERRIKFFKKRKEIKVPDTKVIPTHFKSGKITEK
                     YSDPFKQAQYAYSKIKEVK"
     gene            63717..65267
                     /locus_tag="ACHGNS_000441"
     CDS             63717..65267
                     /locus_tag="ACHGNS_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN6936422.1"
                     /translation="MAGKQVTVIPMKPRQWAAENTEEKPKLKVAAYCRVSTEMEEQAS
                     SYEAQVQHYTDYIQRNPDWELVGIFADEGISGTGTKKRDGFNRMIEACQNGEVEYIIT
                     KSISRFARNTVDCLQYIRQLKELKIAVFFEKENINTMDAKGEVLLTIMASLAQQESQS
                     LSQNTKMGVQYRFQQGQLRINHNHFLGYTKDEDGNLVIEPKEAEVIKRIFREYLEGSS
                     LQEIANGLMADGILTGGKRKLWRGEGVRLILRNEKYMGDALLQKTYTTDFLTKKRVKN
                     DGSYAQQYYVEDSHPAIIPKDIFMQAQQELDRRKSMKNKKSQCFSGKYALSGITVCGD
                     CGNAYRRVHWKNRGTVWRCKSRVDKREHNCTGRTIYETDLHGAVIKAINETLIDRDAF
                     LQQLNDNINSVMTDGLTDRLAELDSQLKELEAEIISLAVGGQGYDELASQIFSLRDER
                     DAVAKQIAANTNLQQRVDEMVVFVKEHDVINEYNEVLVRRLIEKVTIFEKNIVVDFKS
                     GVRVTVEI"
     gene            65390..>66404
                     /locus_tag="ACHGNS_000442"
     CDS             65390..>66404
                     /locus_tag="ACHGNS_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936423.1"
                     /translation="MSQWNTIDELRDIFIRKYTDSELSNELEKACSEEYELMRDYNGR
                     QILELLQNVDDAYGDIKSENDASNDEVKVQITYKNNILEVGNTGTSFSKDTIERLCLG
                     RASNKSSQNIGNKGTGFRSLLNDAEWVELYSGEFAIRFSEEFTKKLFEQYVSRDSDKF
                     SELIFEQKKNWKKEDYDLCFPIMNCPQPINKIDSGFDTLIRVKLKETNLSKGTSVSKQ
                     LQQPFYKSLLFLPNITKIVIETTDDEKISEKIVDGNDVLIEKSDVTGQSPSEEYFVFD
                     KESMIGDKVAKIALAVSKDSDYDYSKEKLYCYFPVRNFGTPIHALINAPFITNNSRDD
                     VPDD"
BASE COUNT        21300 a        12008 c        15056 g        18040 t
ORIGIN      
        1 tattatttgt attgaggtga tcgtgaaaat gcttacagag gaacgtagac aaaaaatcct
       61 agacttgctt gaacagcaga aaatcgtgaa atcacaagat cttgtaaacc tttttaatgc
      121 ttctgaatca acgataagac gtgatttaca agagctggag aatagtgggt ttctagaaag
      181 agttcatggc ggagctaaga aagagcaatt attgaatttt gagcagaata tgagtgaaaa
      241 atcactcaaa aacgttcacg aaaaacagca aatcgccaaa tcggcagctg aaaccattaa
      301 tgacgaagat gtcatttatt tagacgctgg ctctactacg ttagaaatga ttccttttct
      361 gcaaggaaaa cagatcacag tagtgacaaa ttccgttcaa catgcggcaa atttagttga
      421 tttggagctt gctaccatta ttttaggtgg aacgataaag ctggcaacta atgctgtttt
      481 aggatctaat gcgatagaac aactaaaaaa ttttcatttt aataaagcat ttatgggaat
      541 gaacggtgcc catcttgaac aaggattcac gactccggac ccagaagagg cagctgttaa
      601 acgtttagcc atgaaaaatt ccgaagagac atttgtattg attgatcata ccaaattcaa
      661 ccagttgtcc ttcacctctg ttgcaccttt ggaagcagcc acgatcataa ccgagcgttg
      721 tccattagaa ttcgttaatg aattccgtga aaagacaaca attaaggagg caatacaatg
      781 atttacacag taacacttaa tccatcaatt gattatatcg tacgcgtaga tgggttaaaa
      841 ctaggcgatt tgaaccgaat gaaggaagac ttcaagttac ctggaggaaa agggatcaat
      901 gtttcccgta ttttgaaacg gatcgattct caatctacag cactagggtt tttaggaggg
      961 ttcactggcg aatttatttc gaagtggctt gaaaaagaac aaatccagtc tttgttcaca
     1021 accgtaaaag aagatacaag aatcaatatc aaactaaaat cagatactga aactgaaatt
     1081 aacggtttgg gcccatctat ttcagatgaa gaaatcgatg agttgaagca agcattagga
     1141 acagtaggac aaggagatat cgtcgtattg tcaggaagcg cgccggctag cctgcgaaaa
     1201 ggattttatc aagaactgat tgatatcatt cgagaaaaag gtgcagattt cgtgattgat
     1261 acaacaggtg atgacctaaa aaatgcactt gttaagaaac cattgttgat caaaccaaac
     1321 aatcacgaat tagctgagct ttacaataca accttccaat ctgtagaaga aatcattccg
     1381 tatggtaaaa aattgctagc tgaaggggca cagaatgtgc tgatctcaat ggctggagac
     1441 ggtgctttgc tcttcacgga acaaggaact tataaaacga atgtattagt tcgcccattg
     1501 aaaaattcag taggtgcagg ggattcaatg attgccggct ttattggggc ttacagccgt
     1561 acgaaagatc caatcgaagc ctttaaatgg ggagttgctt gcggaagcgc aacagctttt
     1621 tctgatgact tggcagctcg cgaattgatt gatgagttgc tgccagaagt tgaaatcaca
     1681 aaaattgatt agtttcaagg aggataatca tgaatatcaa ggatttaatg gtcaaagaag
     1741 cgatgatcat ggatttgcaa gcgactgata aaaaaggcgc aatcgatgaa atggtccaaa
     1801 aaatgtttga tgccggccgg atttccgata tcgaaacata taaagaagga attctagccc
     1861 gcgaagcaca aacttctaca ggtcttggtg atggaatcgc catgcctcac gcgaaaaaca
     1921 gtgcagttaa agaagcaaca gttttattcg caaaaagcca aaaaggagta gattatgaag
     1981 ccttagacgg acagccaact tatctgtttt tcatgatcgc agctccagaa ggcgcaaacg
     2041 acacacattt gcaagcatta gcagcactat ctcgattgct gattgatgca gatttcgtag
     2101 gaaaattaaa agaagcaaat acaccagaag aagtccaaga cttgttccaa acagctgaag
     2161 agcaaaaaga agcagaagaa aaagcagaac aagaaacaca agctgcagaa gcttctgctc
     2221 atccagaaag aaaattcgtt gttgccgtta cagcttgtcc aacagggatc gctcacactt
     2281 acatggcaga agatgctctg aagaaaaaag cgaaagaaat gggtgtggag atcaaagtcg
     2341 aaacgaacgg ttcagaagga atcaaaaacc gattgactgc tgaagacatc gcccgtgcag
     2401 aaggtgtaat cgttgcagca gacaaaaaag tagagatgaa tcgctttgac ggaaaagaac
     2461 tgatcaatcg tcctgtcagt gatggtatcc gtaaaccaga agagctgatc aatctagccc
     2521 ttagtggtac tgcaccagta ttccatggtg atggaaaaga aagcagttca gaagaaggaa
     2581 atgcggatgg tactatcgga caaagaattt acaaagattt gatgaacggc gtatcacaca
     2641 tgttaccttt cgttattggt ggaggaatcg ctattgcgct ttctttcatg atcgaccaat
     2701 ttattggcgt gccacatgat caattatcaa atctagggaa ttacaatcaa gctgctagct
     2761 ggttcaacca aatcggacaa gctgcatttg gtttcatgct gccggtatta gcaggattta
     2821 ttgcttcaag tatcggtgac cgccctggtt tgatcgttgg atttgctgct ggtgcattag
     2881 ctaatacagg cggtgcagga ttcttaggtg cactgattgg tggattctta gctggttatg
     2941 tcatcgtatt tttacgtaaa ttattcaaga acttaccaaa atcattggat ggaattaaaa
     3001 cgatcctctt ttatccagtg tttggcttac tgattactgg tttcttgatg ttgctggtca
     3061 atgtccctat gaaagcaatc aatgatgcat tgaacggttt cttgacaagt atgagtggtt
     3121 cgaatgctgc tttacttggt gcattattag ctggaatgat ggcggcagat ttaggcggtc
     3181 caatcaacaa agccgcttat gtgtttggta cagcaacttt agctacgact gttgcaactg
     3241 gcggaagtgt cgtgatggct tctgttatgg ctggaggaat ggtacctcca ttggcaattt
     3301 tcgttgctac acgtttgttt aagaataaat tcagcaaaac agatcaagat gctggtttga
     3361 cgaacatcgt gatgggacta tcgtttgtca cggaaggatc gattccattt gcagcagctg
     3421 acccgattcg tgccattcca agttttatta ttggttctgc tttaactggc ggattagtcg
     3481 gagctttcgg gatcaaatta ctggctcctc atggcgggat tttcgttgta ttcttactga
     3541 gtcacccggt catgtatttg gtgtttattg ctatcggagc agtcgtatct ggtgtgattt
     3601 atggtttatt gagaaaagct cctgcagcag cagaagcata gaaataaaaa agaagaggct
     3661 ggtttttccc agtctcttct ttttttatag aatcatatag aatcaagtag tgtagatgaa
     3721 tagttgttgt aaaatagttt ttccatgttc gttaaaatga ttgcttcaaa tttgtgatag
     3781 aagatttagc ctcgatttcc tgcttgattc ttggcaagtt tgcttcatca gtcaagataa
     3841 ccaaaatatc tcctacattc atgatcgtat caccgtgagt caagatttcc tgatttcctc
     3901 tgcgtataga gattagcagc atatttttag gccaattgaa gtctcgtacc attaatccgt
     3961 ctaaagaact ctctgccgtt actggaaaat caaaagttgt ccttgcacct tttagtgagg
     4021 cggtctgagt atttccaagt agacgttcca ataatgattc gtaaattgga ttaccgccga
     4081 gaaggtcatt gacgatataa gcaacaagag cagccaatcc taaaggcatc agatgattaa
     4141 gagagccaac catttctgtc actaaaataa ttgctgtcag cggtgctttt ccaatcgctg
     4201 tgaaataacc agccatggca ataacgacaa aatccttgat gtagattgga tccatcccga
     4261 aatcaaccat aatgtttcca taaaagctac cgattaaagc ccccaatgtc aatatcggaa
     4321 ggaaaatccc ccctggaaga ccaccaccgt aagaaatcat ggaaaaaacg aatcgaagaa
     4381 caaacaaacc gataatcata atgattggca aggaagcatg tccaaaagct aaaacgattt
     4441 gattgcctcc gcctagagta ctaggccata aataaccaat tggcaaaatc agcaagaaag
     4501 gaacgaaccc gtgaaaataa gctggtaaag gcaatcggtt gtaaaatttt ggcaaagcca
     4561 gcaagacctt ctgataaata aaacctaaga tgcctagaag tactcctaag atcactaagg
     4621 aaccataata tttcaacggc aaagaaggaa ggtcacctag atataaaaca ggtctcaagc
     4681 cgaaaaagta aagagagacg aagttagcag tgattgctga agcaaatgat gtcagccaaa
     4741 caagtggaga aaaggagtga tggatttctt ctaaaacaaa gagtaatcct gcgataggag
     4801 cattgaatgc tgctgcaagt ccgctacttg caccgctaga aattaaaatc ttttcttctg
     4861 aagatggact ccgaaacaat tgtccagctc cttgtccaat ggatgcgcca agctgtatag
     4921 aaggtccttc tctacctaga aaaaggcccg caccaactga aagtacaccg ccaatgaact
     4981 ttttccataa aacagagaac cagttcattg ataattcccc gcgaagctgt ccctctactt
     5041 gtggaatccc gcttcccttg atatccggtt catttttgac cagataaccc acgaaaaatg
     5101 ctgcaaggat gcagcaaata gctaggccaa ttaagtaaat cggctgttcc cgactcattt
     5161 catagatact tcgaactaaa tcgcttagct tttcgataga taatcgaaac aggctaacga
     5221 cgattcctgt cagcagtcca acaaatattc cttttagaat aaaatatatc tgagtcttat
     5281 ctaatttctt gatttttcct tttttttcca ttcaaacgcc tcgctttctt ttaatttagc
     5341 gcgaaatcaa cgaattgaca aggaaaaatc cataaaaaga aacaaatgat tttgaactag
     5401 ctagtttaat gaaagccaag aaaatgctat aattaacaag attgataaaa gaaaggatgt
     5461 tgttaatgtc atccaaagct gaattattaa acgggatgaa cccccgtcag aaagaagctg
     5521 ttcttcacac tgacggacca ttactgctaa tggcaggtgc aggaagtgga aagacgagag
     5581 tgctgacaca ccgcattgcc tatttgattg aggaaaaaga agtcaatcct tggaacatcc
     5641 tagcgattac ttttaccaat aaggctgcaa aagaaatgaa agaacgtgtc aatgcgattc
     5701 tcgcatctgg aggcgaagat gtctgggtat cgacttttca ctcgatgtgt gtgcgtattt
     5761 taagaagaga tgttgatttt atcgggtata accggaactt tacaatcatc gattcttctg
     5821 aacagctgac cttgatgaaa cggatactaa aagaactgaa cattgatccg aaaaaatatg
     5881 atcctcgaag tatattagga acaatctctc aagcaaaaaa cagcctgcag acccctcagg
     5941 attttgcaaa aatgcaaggc agttattacg aagagattgc ggcaaaatgt tatgctgctt
     6001 accaaaaaga gcttcaatat aatcaatgta tggactttga cgacctgatc atgaatacga
     6061 ttcgattatt tgaagaacat cctgattcac tcacatatta tcaaaacaaa ttccattata
     6121 ttcatgtaga tgagtaccaa gatacaaatc acgcacagta tacgctagtc aatttattgg
     6181 caggtagatt taggaacctg tgcgtcgtgg gagatgcaga tcaaagtatt tacggctggc
     6241 gcggtgcaga tatgcagaac atcttggatt ttgaaaaaga ttatccagat gctgcagtaa
     6301 ttttgctaga acaaaactat cgctccacga aaaacatcct tagcgcagca aaccaagtca
     6361 ttgaaaacaa tagcaatcga aaaccaaaaa acttgtggac agaaaacaaa gaaggaaaca
     6421 agatcactta ctaccgtgca gataatgaac gagacgaaac ccgttttatc gtagatcgga
     6481 tgcaagaaga gattcgaagc aatcatcgga attacggcga ctttgccatc ttgtaccgga
     6541 cgaacgcaca atctcgtgta atggaagaaa ctctgttgaa agcaaatatt ccttataaaa
     6601 tggttggagg gcacaagttc tacgatcgta aagaaatcaa agatatttta gcgtatttga
     6661 atgtgctggc aaatcctcaa gattcaatca gttttgagcg tatcgttaat tcgcccaaac
     6721 gaggcatcgg accaggatct atcgaaaaac tacgcagttt tgcttctctt catgaatggc
     6781 cactgcttga agcagcgcaa aatgtggatc tggcaaatat cggtggaaaa gcaggacagc
     6841 aattaggtgc atttggagag atgatccaag aagtcactca gatgatccca tacttgacag
     6901 tgacagaatt gacgaaagag gttttggatc gtagcggata tctagaagac ttaaaaatcc
     6961 aaaatacatt agaagcacaa gcacgcatcg aaaacttgga ggaattttta acggtcacac
     7021 aagaattcga caaacaattc gaacagcaaa atgaagaaga cgctgatgca ccagaagaaa
     7081 aactaacggt cttcttaaat gatctggcac ttgtttccga tattgacaat ttagaagaag
     7141 atgcttccca agtgacattg atgactttgc atgcagccaa aggattagaa ttcccagttg
     7201 tattcctgat tggtctagaa gagggggttt ttcctttatc tcgagcattg atggaagaaa
     7261 gcgaactgga agaagaacgc cgtctcgcat atgtggggat cacgcgagca gaagaagcgt
     7321 tgtatttaac aaatgctttc tccagaacat tatatggacg gacgcaatat aaccgacctt
     7381 ctcgttttgt agaagaaatc gatcaggagt tactggaaat cgaaggcatg cgtcctacgc
     7441 ctaaaaaaac acctgtattc gctaaaaaaa cagcatatag ctataaacag ccagagacag
     7501 cggttgttcc ctctaaaagt gctactggag gggaaaataa cagctggaaa cctggagata
     7561 aagtcaaaca caaaaaatgg ggtctaggaa cagtcgtccg agtaagcggg acgtcgaagg
     7621 atctagaact agatgtggct ttcccaagtc aaggagtaaa acgtcttttg gctgcctttg
     7681 cgccaattga aaaagcttag tagtggaggg taaatgatgg aacatgaaat gacgattgac
     7741 gaagctgcta aacgagcaga agaacttcgt acaagactga accaatggtc tcgcgaatac
     7801 tacgtagaag ataaaccaac agtggaagac tatgtttatg ataaagaata cgcagaatta
     7861 gtagcaattg aggaacaata tcctgatttg atcacgtctg attctcccac tcaacgagta
     7921 ggcggaaaag tattagaagg ttttgaaaaa gtcactcatg atattccgct ttacagtttg
     7981 aacgacgttt tcagcaaaga agagctgata gcttttgatc aacgtgtaca aaaagcagtg
     8041 ggacgtgtag ttgactattg ctgtgaacta aaaatagatg gactttccgt ttctttgcgt
     8101 tatgaagatg gaaatttcgt gagaggtgcg actcgcgggg atggaactgt aggagaaaac
     8161 atcactgaaa acctaaaaac tgtccggtcg gtcccaatca aattaaaaga accaatgaac
     8221 atcgaagtaa gaggcgaatg tttcatgcca aaacggtcat ttgtccaatt gaaccaagac
     8281 agagaagcag agggaaaaga tatctttgcg aatcctcgga acgccgcagc tggttctttg
     8341 cgtcagttag attctaaaat cacagcgaaa agaaatctgg acactttttt atacacagta
     8401 gctgatttcg gtcctatgca agcaaaaacc cagtatgatg cgctagaaga gctagaaaaa
     8461 attggattcc acacgaatcg ggaaaaacgt ctatgtcatt cgattgatga agtgtggtca
     8521 tacattgaag aatatcatga caaacgtgtg gatttgccct atgaaatcga tggaatcgtc
     8581 atcaaagtga atgaattctc gctacaagat caacttggtt ttactgtcaa agcaccgaga
     8641 tgggcagcag cttacaagtt tccgccagaa gaagttgaaa cgctaatcga aaacatcgaa
     8701 tggacagtcg gtcgaacagg ggtagtaacg ccaacagcta tcatgacacc agtccgagta
     8761 gcaggaacga cagtaagccg tgccagcctg cataacggtg attatattaa gttgaaggat
     8821 atccgcttga aagatacagt tctgatttat aaagcgggag acatcatccc agaagtttcg
     8881 caagtcgtgt tagataaacg acctaaagac agcgaagaat accaattgcc tacacattgt
     8941 ccagtttgtg gaagtgaact tgtccatttg gatgaagaag tagccttacg gtgcatcaat
     9001 cctaaatgcc ctgctcaaat gaaagaaggt ttgaaccact ttgtttcaag aaatgcgatg
     9061 aatatcgatg ggttggggcc gcgcgtacta gaacagatgt atgataaaaa actagtagca
     9121 gatgttgctg atctttacaa attgacagaa gaagagttat tgacgttgga caaaataaaa
     9181 gaaaaatcag caaataatat tttgacagca atcgacaaca gcaaagataa ttcggtcgag
     9241 cggttgattt tcgggttagg aatccgacat gtcggagcga aagcagcaaa aattttagca
     9301 gaacatttcg gtgatctaga aacattgagt aaaagtgact atgagtcgat tattgcgctt
     9361 gatacgatcg gtgatatcat tgccgacagt gtagtcactt atttcagtaa cgaagaagtc
     9421 catgaattga tgaatgaatt aaaacaagca ggcgtcaatt tcgaatacaa agggcttcga
     9481 aatgcccaat tgcaagaagt cgagtctcct tttaaagaaa aaacagtagt ccttactggt
     9541 aaactaacac gatttactcg tgaagaagca aaagagacga tcgaaaatct tggcgggaaa
     9601 gtgactggta gcgtatcgaa aaaaacggat atcgtggtag caggagaaga tgctggcagc
     9661 aagctgacca aagcacaaga attaggaatc gaagtttgga cagaagatca aatggcagat
     9721 gcattggcta aaagtcgctc agtagaagaa tgaaaaatga gttttttcag acaaatttca
     9781 ataaacaatt tcagacacgg cactcttgtg ttaaaataga gagcaaagtt tttaactcgt
     9841 ttagaaagaa ggggtagtat ggcgattagt gaagaacaag tgaaacacgt tgccaaatta
     9901 gcaaaacttg cgttcgcaga tgaagaactt gcaagtttta ccgaccaatt aggtaaaatc
     9961 atcgatatgg ttgaaatgct ggaagaagta gatacagaag gtgttccatt tacgtcgaat
    10021 gtggttgaaa cagtaaacgt gatgcgacaa gatcgggcgc aagctggatg gaaccgcgat
    10081 gaattattga aaaatgtgcc ggaaacagaa gacggcttta tcaaagtacc cgcaatcatc
    10141 gataatgggg aggctggcgc ataatgacag aattacacaa tcgttctcta gaagaactgc
    10201 atcatatgct tgtatcgaaa gaaatcactg tacaagattt gacaaaagca acatttgaac
    10261 gaatcaaaga aacagagcct gaggtcgaag cattcattac attgaacgaa gaaaaagcat
    10321 tggaacaggc aaaagcttta gatgaaaaag ggattgatga aagcaacgtc cttgctggta
    10381 ttccaattgg tatcaaggat aatatcgtaa caaaagatat cttgacaacc gctgcatcta
    10441 agatccttta caattttgaa ccaatttatg atgcaacggt catggataaa atctatcaag
    10501 cagatatgat ccctgttggt aagctaaaca tggatgaatt tgctatgggt ggaagtacag
    10561 aaacttctta tttcaagaaa acaaaaaatg cttgggaccc aacaaaagta cctggtggct
    10621 cttctggtgg ttctgcagca gcagttgcag ctggacaaat tccagtttct ttaggtagtg
    10681 atactggggg aagtatccgc caaccagcag cttttaacgg catcgtaggg atgaaaccta
    10741 cttatggccg tgtctcacgt tttggtttga ttgcctttgc ttcatctctt gaccaaatcg
    10801 gaccaatgac ccgtacggtg aaagataatg cattagcatt gacagctatc agcggttatg
    10861 acgaaaaaga cggtacttct tcaggcgttt cagttcctga ttttgcagag ggactgacag
    10921 gcgacatcaa aggaatgaag attgctttac ctaaagaata cttaggcgaa ggtgtggccc
    10981 ctggcgtaaa agaagctgta ttgaaagcag cagaaacatt ccgttcatta ggtgctgttg
    11041 tggaagaagt cagcttgcct cattcaaaat acggtgtggc tgtttattat atcattgcct
    11101 catcagaagc aagttccaac ttgcaacgat tcgatggtat ccgctacggt tatcgctcag
    11161 aaaatgtgaa atcattagat gatgtatatg tcaactcacg ttcagaagga ttcggtgaag
    11221 aagtaaaacg ccgtatcatg ttaggtacat tctccttaag tgctggttat tatgacgctt
    11281 acttcaaaaa agcgggacaa gtccgtacat tgatcaaacg agattttgaa aaagtctttg
    11341 cagattatga cttgatcatc gggccatctt caccaacagt tgcattcgga atcggtgaga
    11401 acattaacga tccaatcaca atgtacatga gtgacatctt aacgatccct gtcaacttgg
    11461 ctggacttcc aggtatgtct gtaccggcag gtttatcaga aggattacca gttggattgc
    11521 agatcatcgg taattatttt gatgaaaaaa cgatgtatca agcggcatat gcgtttgaac
    11581 aagcaacaga attccataaa caacaaccag ctattttagg agggaaagac gaatgaattt
    11641 tgaaacagtc attggactag aagtccacgt cgagttaaaa accaactcaa aaattttctc
    11701 tccagcgcca gctcattttg gtgcagaacc aaacagcaat acaaacgtga tcgactgggg
    11761 ttatccaggc gtgctgccag taatgaataa agctgcgctg gaattcggga tgaaagcagc
    11821 tttggcatta aactgtgaaa tctctaaaga tactcatttt gaccggaaaa attactttta
    11881 cccagataat ccaaaagcat atcaaatttc ccaatttgat caaccaatcg gtcatgacgg
    11941 atggatcgaa atcgaagtag aaggtaaaaa gaaaaaaatc cggatcgaac gtgtccattt
    12001 agaagaagat gctggaaaaa acatgcatgg tattggcggt tattcttacg ttgatttgaa
    12061 ccgacaaggc acgcctttga tcgaaatcgt ttctgaagct gatatgcgtt caccagaaga
    12121 agcatacgct tatctagaag cattgcgatc aattatccaa tttacagaag tcagcgatgt
    12181 gaaaatggaa gaaggctcta tgcgctgtga tgccaatatt tctcttcgcc cttatggaca
    12241 agaagagttc ggtacgaaag cagaattgaa aaacttgaac tctttatctt tcgtgaaaaa
    12301 aggtttagct tttgaagaaa aacgtcaagc aaaagttctt ctttcaggag gagaaatcca
    12361 acaagaaaca cgccgttttg acgaaacaac gaataaaaca gttttgatgc gtgtcaaaga
    12421 agggtctagt gattatcgtt acttcccaga gcctgatatc ccgaaattcg tgatcgatga
    12481 cgaatggatt caaaaagtaa aagctagttt gcctgagatg ccagcttctc gtcgcgaacg
    12541 ctatatccgt gagtttggac ttccagagta tgacgcaatg gtattgacac ttacaaaaga
    12601 aatgtctgat ttttttgaag ctgttttgga aaatggtgca gatgccaagc aagcatctaa
    12661 ctggttgatg ggtgaagttt ctgcttactt gaacagtgag aaactggaat tagctgagac
    12721 aaaattgaca cctgagaact tagcaggaat gattcgtttg atcgccgatg gaacgatcag
    12781 ctcaaaaatc gccaaaaaag tcttccgtga attgatcaca aatggcggag aagcccaaga
    12841 agtagttgaa aaaaatggtt gggtgcaatt atctgatccg aacaaactac tgccaatcat
    12901 taatgaaatt ttggataaca accaacaatc tgtcgatgat ttcaaaaatg gaaaagatcg
    12961 tgcggttggc ttcctagtcg gtcaaatcat gaaagcaact aaaggacaag caaatcctgg
    13021 tgtcgtgaat aagctactta aagatgaatt agcgaaacga tagaggctga aaatgaaaaa
    13081 agcacgcgta atttataacc ccacctcggg aaaagaatta ttgaaaaaaa atttggcgga
    13141 tatcctacag gcattagaag aagcaggatt tgaagccagc gcctatgcaa ctacaccaga
    13201 accagattca gccaagaatg aagcatgtag agtcgcagaa ctaggttttg atctagtagt
    13261 ggcagctggt ggcgatggca caatcaacga agtggttaat ggaatcgctc cgcttgaaca
    13321 ccgtcctaaa atggccatca tcccagctgg tacaacgaac gactatgctc gagcattaaa
    13381 aattcctaga gataatatta aagcagcagc agaagtgata aaaaaacacc aaaccgtaaa
    13441 aatggacatc ggcctttctg ataacagtta cttcatcaat atcgctgcag gtggctattt
    13501 gacagaattg acttatgaag ttccatcaga actaaagagt atttttggtt atcttgctta
    13561 tcttgcgaaa ggagcagaga tgctccctcg agtaaagcca atcaaaatgc gcttgaagta
    13621 tgatgaaggg gaatatatcg gaaatgcttc gatgtttttc ctagggctga cgaactctgt
    13681 tggaggattc gaacagatcg cgccagatgc caaattggat gatgggaagt tttctttaat
    13741 cattgtgaag acagcaaata tctttgagat cttgcatatt gcagcactca tgctgaatgg
    13801 cggtcgacat gtcgatgatc caagaataat ctataccaaa acaagcagtt tgttcgttga
    13861 aacagaagat gatatcaatc gaccagtaat gatcaactta gatggtgaat acggcgggga
    13921 agcaccgatg catttccaaa atcttcatca acacattgag ttctttgcca ataccgatca
    13981 gattcctgat gaagcaatca caggtacaga tgaagaagaa ttagaggttg tcagcaaaga
    14041 attcgtaaaa gaagtcgaac gtttaacgga tgaagatatc gacggtgacg gaaaaatagc
    14101 agacgaaaca cctaatcaaa agaattaatc aaaagccggt gctgaacgta gtaaggtcag
    14161 tgccggtttt agttgtgtaa ttagctgatt tatagtagag ttcagtagga ttgaaaaata
    14221 ggcttaaagg agcaaaatat gacagaacag caaatagtaa agaaaaacga acgatataca
    14281 gtcgaaatcg tcgacttgag ttatgaagga atgggggtag caaaaatcga tggttatcca
    14341 ttatttatcg aaaatgcact ccctggagaa gaagtagaaa tactagtagt aaaagcaggc
    14401 aataagtttg gatatggaaa agtcgaaaca tttattacac aatcacctga tcgtcagcct
    14461 gtagaaaaca atgcattact tcgcataggt atcgcacctt tagcccattt gaagtatgaa
    14521 caacagcttg tcttcaaaca aaaacaagta gagcatgtaa tggcgaagat tgctaagatg
    14581 ccagaagttc ctgtacttcc aacggtcgga atggcagaac cattcggcta ccgtaataaa
    14641 gcacagatcc ctgttcgccg tatcgatgga cagttagcca ccggttttta caaaaagaat
    14701 agccatgatc tagtccctat cgaagatttt tatatccaag atcctgtcat cgatcaagca
    14761 atcaaagtag tcagagatat cttgcaacgg ttccaagtac gaggatataa tgaagaaaaa
    14821 aatgaaggac agcttcgtca catcattatc cgtcgtggtc attattccca tgaaatgatg
    14881 gtcgttttag ttactcgtaa agaaaaattc tttaaatcca aggaaattgc tgaagctatt
    14941 catgaagaat tgccagaagt ggtttcggtc atccaaaata tcaatgaaga aaaaacaaat
    15001 gtgattttag gagacaaaga aaaagtttta tatggcagag actatatcga agatcagcta
    15061 ttaggaaaaa cctatcgaat ctcagcaaag tcattttatc aagtaaatac tcaacaagca
    15121 gagtatctgt accaaacagc catcgagttt gccgagttag ataaaaaaga cattgtgatc
    15181 gacgcttatt caggaatagg tacgattggc ttgtctttag cagacagagt ggataaggtg
    15241 tatgggatgg aagttattcc agaagcaatt gaagatgcac agttcaatgc tttaaataat
    15301 aaaatcgaaa atgttcatta tgaagtcgga aaagcagaaa aagttatgaa aaaatggcaa
    15361 gaaaaaggga tcaaaccatc tgtgatcgta gttgaccctc cacgaaaagg attagatcat
    15421 cattttattg aatcagcaat cgaaatgtca ccagaacgaa tcgtctatat ctcatgtaat
    15481 ccggcaactt ttgcaagaga tgctaagtta tttgcagaag caggttatgc cgtgaagaaa
    15541 gttcagcctg tagatctttt cccgcaaacc catcacatcg agtgtgtagg actgcttaca
    15601 cgtgtggaac gttgatatga caaggattag agcgattcgg gatggtttcc ggattgctct
    15661 tttttcgtgt tgaaatgacg atttttgggt gagcgtagag ttgatggaaa tgctatggag
    15721 ctttgagttg ataggagtta caccgtagac ttgataggtt agatgatttt gataacggtt
    15781 acgtgatata atatattatc gagttttcaa gggagacaaa cttatgaagc aaatttttat
    15841 attttcagat acacagaaag ataatatttc ttattttgaa cgcgagccag caatcttggt
    15901 ttcaatgagt cgtagtgatt tgccgtattt gaaggaacag gatttttcaa attatccagc
    15961 tgtttatgtt ttgattggtg gtaataagcg ttacgtgggt caggcagcag ggcaatcaat
    16021 ttcacttcgt ttatctcaac actttctcaa ggaatataaa gcttgggttg aatcagtttt
    16081 attctttaca agagcagatg ggaaaatgtc aaaagcagat acagattatc ttgaaaaacg
    16141 acttatccaa gatttccaag aaaaatctga ctatgaaatg atgaattcaa ctgctggtaa
    16201 tacttcgtat attgataaac ttcaaaaagc aaaatcggat cagctatatg acacagtgtt
    16261 tgaaattata gatgaaattg caaatatcga tttgtttggt acttccgaag acagtgaaac
    16321 ccaaatttat gaatttagtt catctggaca atatgaaatt gagtatgatg ggaaaaagat
    16381 atcttcgaaa tctgctagag gtttattggt tgattttgtt tctcagctat ttgcggatgg
    16441 aaactacaac aatcaactgc aagaattaat tgtcgatgag acgccgtcgt tcgctataat
    16501 tttaggacgt aaaccatcca tgtataacgg aagaccaaat tcagccaggg tagctgatgg
    16561 agtatgggtt tatgcaaatt tcaacaaaaa gaatgtcaga atcaaaattg ataaactagc
    16621 tcgtcaattg gggattccgg ttaaaataaa gtggaattaa tatttgttat ctcgccttta
    16681 gtaaatctat gctgaaggct tttattttag aaaaatagtt tgcgttcgcg aaaatatttc
    16741 atgtttcgta taataatgat agctcataga tgatataatt gaagatacca gtagaataga
    16801 ggtgtagtat gtcaatttct tataataaat tgtggaagtt attgattgat ttgaatatga
    16861 acaaaactca acttcgtgaa atatctggcg tcagtcccaa tgtaatagca aaactcggta
    16921 aaaatgagtt ggttgcaatg gagtcattat tgaaaattgc tgctgcctta aatgttgatg
    16981 ttagtgaaat aattagttca gatgttatag atgcggagga tgttaaataa atggcaaaaa
    17041 ataaagtttt aaacatcact gaaattgaag atggtgtaaa aaaaatactt tcaaataggg
    17101 gcgatgattt catcactcaa tttttgtcct tgtacgatat ccccaaaacg tcgattacac
    17161 gtgcaaaatc aaaattcgat aatggtgaac catttgtgat taagaacaaa cttcattatg
    17221 aagaaattga aggcaacgtt gtcattgcga ttgatgcgat tgctcaatca atcaaagagc
    17281 aaaaatctaa accacgatat atcttggtaa acgattattt agatattgct gcaattgata
    17341 cgaaaactca tgaaactttg aatattccaa ttgaagagct accggctaat gctgactttt
    17401 tcttagcgtg gaatggcatt gagaaggctg attatcaagc tgaaaatcca gctgaccgaa
    17461 aagctgctga acgttttgca aaattgtatg atgtggtggc taaagataat cctgatgcca
    17521 acgagcatgc atttaacttg ttcttgattc gagtgctgtt cttgttgttt gcagaagata
    17581 caggaattat gaataagggt tcctttacaa atgttctaaa aacaagaacg gctgaagatg
    17641 gttcgaattt taacgaagta atcaaagaac tctttgcaat cttggatatt aatgagttag
    17701 ctcgcaatgg caaggatgag tggctacttg aatttccata tgttaatgga aaactgtttg
    17761 gtgagccaca tgtggatttg accttttcaa aagtttcaag agagctgctt attgaagcag
    17821 gggaattatt gaattggaat gaaatcaatc ctgatatcct tggcgcaatg attcaatcag
    17881 ttgcttcagc agaagaccgt cacgttgccg gtatgcatta tacaagcgtg ccgaatatca
    17941 tgaaagtcat caagccgtta ttcttagatg atttgacaga gtcatttgaa actttaaagc
    18001 ttcgcagtga agaaaacgag ctgaaggaca ttactgaaaa aacaagaaac gataataaga
    18061 gaacaatcat caatgatcta agtgctttgt tgacgcgaat ttctaatatt aagtttttgg
    18121 atcccgccag tggttctggt aattttctga tcattgctta taaggaaatc cgtagacttg
    18181 aaattaagat tttgcaattg ttgggcgact tacaaaataa tgattccatg ccactatcag
    18241 tgattcatct tggtaatttt aatgggattg aattggatga ttttgcgcat gaagtagcaa
    18301 gactttcatt atggattgct gaacatcaaa tgaataaaga gcttgaagaa gcaataccag
    18361 gtaccattgc tgagttgtta ccgttaaaag atgctgggaa tatagtgtgt gccaattctc
    18421 ttcgaattga ttggaatgag gtgttgcgac tcaaagaaaa tcatgaaata tatataatgg
    18481 gaaatccacc gtatctgggc aaaagtcttc aaaatgctga acaacgtgaa gatatggaaa
    18541 aacttgttgg cgatattcga aaatataaat tgcttgatta tatttttggg tggttttgga
    18601 aagctaaaca atatattgca acatctcatg gctcgttcgc atttgttact actaattcaa
    18661 tttgtcaagg tgaacaagtt agtatccttt ggccgtacct tttaaaagat gaggtaagaa
    18721 tttcatttgc atatcagagc tttaagtgga acaacaatgc aaagaataat gccggtgtga
    18781 ctgttgtcat tattggtatg acaaataatg tgaatattca tcctaagtta tatttagaga
    18841 atgggtcagt aatgagtgtt tcaaatatta atgcatattt agctaatggt gaaaatgtat
    18901 ttgtcaacga atcaagaaat agtatttcaa atttaccctc tttgaaattt ggcagtatgg
    18961 caaatgatgg tggtaatcta gttttttcag tggcagaata ctggaatttg attcaaaaat
    19021 atccagatat taaacattta tttaagaaat atttaggatc aacaggatta ataaatggag
    19081 acttaagata tactttatgg ttgaccgagg aagatgcaac tagatacaaa caaaattctc
    19141 ttattaaaga aacaataaaa aatgtaaaaa gttataggga aaaaagcaag agaaaagaaa
    19201 ctcgagcact agctgagaag ccatggtcat ttggtgaagt tcgttatgat gaaaccagtg
    19261 agaatagtat tctaattcct cgtgtgtcat cagaaaaaag agattatgtt cctatgggta
    19321 tagttgggag cgatactata atttcagatt cggctatggc aatctataac gctccaatgt
    19381 ggctgcttgg tctgttagaa tcccgaatgc atatggtgtg gctgcgctct gtcggtggga
    19441 aattagaaac tcggtaccgt tattcagccg ggttagtcta taatactttt ccgattcagg
    19501 atttatccgc ccagcgtaaa aatgaaatga ctcgcgtgat gacagaaatt cttgatttac
    19561 gagaatacga aggcggcacg ttagccgatt tgtataataa agacagtatg ccagaatcat
    19621 tacgtaagaa acaccaggaa ctggatggga ttgttgatag agcttatcag caacgcccgt
    19681 ttgaatctga cgaagatcga cttggaacat tactaaaact ctatcaggag atgacaatca
    19741 atgactagta atttatttga aattaatttt gataaaaatc aatcaaaaac aacaaatgac
    19801 ctcggtatgc gtgaaatgca agaacgtgtt tatgaaaagc gtgcgtcaca gtatttgttg
    19861 gtaaaatcac caccagcttc aggaaaatca cgtgcattga tgtttgttgg cttggataaa
    19921 ctccataatc aaggaattaa aaaagtggtt attgcagttc ctgaacgctc aattggtaaa
    19981 tcatttaaag atactgaatt atcgaagttt ggtttttatt gggattggga agtaaagcca
    20041 agtgacaact taactattgg gggcggtgaa aaatcaaagg ttcaacactt tgttaaattc
    20101 atgaactcga ctgacccaag tgataatatc ctgattgcca cacatgctac gttgcgattt
    20161 gcttttgaag agttggacga ttcattattt gacaatacgc tattagcgat tgatgagttc
    20221 catcatgtga gccgtgatga taaatctgtg ttaggtaatg ccttacgtag catcatgtca
    20281 aactctaatg cgcatatctt agcaatgact ggttcatatt tccgtggcga ctctgttcag
    20341 attctggaac cagccgacga agataaattt gataaggtca cttacactta ttatgagcaa
    20401 ctggaaggat ataaatatct gaaatccttt gcaatggggt atagtttcta tcgtggaaaa
    20461 tatacagatg ctttggataa agtaattgat gtcaacaaaa aatcaattat ccatattcct
    20521 aatgttaatt ctggtgagtc tactaaagat aaatacgcgg aagttgatga aatacttgat
    20581 ttattgacgg acggtggtaa tatcacacag aacgaggatg gtatttatga aatcgaacgt
    20641 ccagatggta aaaaactatt agtggccgac cttgtgaatg aagaaggacg tgaaaaagtc
    20701 tcagcatatc tagcaagtat tacggatgac attaaagatc ttgataagct tgatatcatc
    20761 attgcacttg gaatggcaaa agaaggcttc gattggccat ttgcggaata tgcactgaca
    20821 atcggttatc gtaactcatt aactgaagtg attcaaatca ttggtcgcgt aactcgtgac
    20881 agccccaata agactcatgc acagtttaca aatcttattg cgcagccaga tgcacaggat
    20941 gacgaagtat tattcgcggt taacagtatt atgaaggcaa tttctgcatc tctacttatg
    21001 gaacaagtgt tggcgccagt ttacaagttt aaaccaaaag atggtaagcc agacaaacca
    21061 ggtgatcttt caatcagagg actgaaacct gctgatacga cggaacgtac aaagaaaatc
    21121 atcgaaaatg atatggaaga tttgaaatct agcattttac agagccaaga tattcaaaat
    21181 gcgattgttt caggtgctga tgcgaagatg attaataaga cgttgattcc acgtgtcatc
    21241 attgatcgtt atcctgattt gacgaacgaa gagttagaaa ccgttcgtca acatacagtt
    21301 gccgatataa atattgcggc tggacggaca acaacttcag aagatggtag ccgcgaaatt
    21361 attaagatgg ccgatcgttt cattaacatt gatgaattga atattgattt aatcgatcaa
    21421 gtgaatccgt tccaacgtgc gtatgaagtg attagtcgtg atattgatgc accgactctt
    21481 cggtttattc aggattattt gtcatcaaat aagtatgatt ttactgacga acaattggtt
    21541 gcggcctacc aagctgctaa gaagttccgt gcagaaaatg gtcgaaatcc tgatcgaaat
    21601 agtaaagatg atggtgaaaa atacttggcc tttgctttgc tacgacttgc tgagataaaa
    21661 cgtgaaagag aggcagcgaa agaagatggc gaatagttta gatagcatct ttgaagatga
    21721 cgcttttgat gatttaacca agcaagttga aaaaaagaaa gttgtaaaac aagacccaga
    21781 agttgctaaa tttcaggaga ttatagactt tgtgaatgaa aacggacgag agccagaaaa
    21841 aactcctgaa tggtctagtg aacgcgcatt gtgggctcgt cttagcggat ttcgaggaaa
    21901 acccgaaagg tctgagaagg ttaagaagta tgacactctt ggtttattgg agaaagaatt
    21961 cgaaccggat ggagagtttc tcgctgattc agttgaaatc gatgacgagt tgaatttaga
    22021 tgatatctta ggctcagatg atatgattga tgatttttca gacttgttgg atgtttcccg
    22081 ctatcgtaga actgtgaacg ctgcagataa aattggccgc agaaaacatg caagtcattt
    22141 tgaaaagtat aagagcttgt tcgatcaagt gcatgcagat atcgcatctg gcagacgaaa
    22201 aattatcccg tttgagcagt atgatattca tgaaaataga ttctacatcc aaaacggcgt
    22261 aatgatgtat atcgtttcca tttcgaagga tacctttatc gctgataatg ggaaaacaaa
    22321 tgccagaatg catgttgttt atgagaatgg gacagagaat aagaatctgt tgttacaatc
    22381 tttagcttcg tctctatact cgactgaacg tcatggaagg atggtgacag agccggtaga
    22441 tgaggaaaca ctggctgaaa gctttggaga agagtttacg acaggttata tctacgtgct
    22501 gaagtcatta agcacgaatc cggatattag caagtggaat aacctgtata agattggttt
    22561 cactcgaaat gatgttgaaa gtagaattgc caatgcggaa aacgacgtga cctacttaaa
    22621 tgcaccagtg aaggtcatgc tttctgctga agtacaaaat gtcaacgctc agctacttga
    22681 gcgcactctc catcatgtat tccaggacaa acaagttgtc tttcaggatg gaaacttcaa
    22741 aaaagctact gaatggtata tggttccatt gaatgatatt gaatcgaaaa tcaatgaagt
    22801 aatcgctgga ttacaaaaat aaatatgaaa atgcactgta ctcatgaatc gaatacagtg
    22861 catttttgat atttaatcgc gaacgatttc aacaatatcc gataaatcac aatcaagtgc
    22921 ttcacagatg cgaagtaaga tatctgttgt tacattttcg cctttattta gttttgagag
    22981 tgttgctgaa ctgatatcag tcatttttct gagttgtgct ttattcagtc ctctgtcgat
    23041 taggagtttc caaagcggat tgtagctaaa cttcatgtaa tcattcctgt tctagttata
    23101 aatatcgtat agatatccat agcgtccatt gtttttatct tcagtaaaga caatctgatt
    23161 tacttgttta tcatcatagt ttagtgctgg caagttcgtt gtattctcaa cgatgataag
    23221 ttgtccttcg ttttgatgat ccataaagta tttgaaaagt ccttctttca tattgtcatc
    23281 gccatcttgt tttttgcgtt catctaatcc aagaagaggg gaatcgataa tgaacacaga
    23341 aggtgtgtac ttagcgtgat tatacaagag atgtcgatat gccattacca ctactgaatt
    23401 gataaacgct gtatatccat gtccgttttc ggtccatttt agatttccat tgattttgat
    23461 gtcgaaaata tccttggcga aaaatgcgtt acgaaattca ttaggtttat aatgcatatt
    23521 acgtaacatg gagtccagta actcagacat ttctttgtag aattctgcat caaatttctc
    23581 tttaggttta taaaggatac gttcgtcttc tggctcattc tcgatttttt caagatcact
    23641 tttaagctta ttttgcattt tttccatcga gattagctgt tgtttaatat ccctgtaagt
    23701 ttcgatttta ttgattcgat cctttaacgt ctcagctttc ggttttagtt catcgttgat
    23761 tgctgcatca agcgatttct ttcttgtttt aagctcatca agctgttcca ttaactcttg
    23821 cagttcaact tcggtatcat gagattcttc cgtcaaatct gataattgag agagcaatga
    23881 atgtaattct gacctagcag tatcgatgta ggttgtttgg acaggtttac ttagttcgcc
    23941 gttacagaat ggacagctat gtgaagtatc atgttcatta tgatcattta ataatcgctc
    24001 accatctacg attagtgtta gtcgttcaat atccgattga tattgataat gtaactgttt
    24061 gtacctattc aaaagaagtt gagtttcatg aagatactgt tccttttgat aaatctcttt
    24121 tatcaagatt ttgctgctgg ctaacgcatc attgatttgc ttttcaattg tttcaagttc
    24181 agcgaacatt ccatcgacaa tttgttgaaa atcatcagag ggagcctcag gtaattctga
    24241 tttagccact tcgtagtttt catataattc agaaaggctt gaattgatgt agttaatcac
    24301 cgagttcctt ctaatttttt tatgttcttt tgtttcgttt tcttccgagt tcgcaaaatc
    24361 tgaaccgtat agcagaagta acaacgatga taaaaaagct gtgcgttggg ttgtttcccc
    24421 aggaaaaaga acagaggcgc tggtattgac tctatcttgt ggaataagtg tgctaggcgc
    24481 taatgtccgc caagttaatt tttgacgttt gaaatcctca ttcttgataa tctgataatc
    24541 atcatcgatg cctaaaagtt gtaaccaaaa atggctaatt ggatttttcc cagatcgtgc
    24601 attatatttt tcataatcta catattcacc tgaactcgtt attttggata ccaaaatgga
    24661 gttgctatca aattctctag ttaatgaata tttgccatta gtcgattgaa taatcagttc
    24721 aactttactg tatccggttg catcggttga aaagggagca gcgtcttttt tcgcgccata
    24781 gagaaaatcg attgccgtaa caatgctagt ttttccggta tttgaagggc cagaaattat
    24841 gtttagtcca ttggtgaact tgacagttgc atcttcacga tctttgcctt ttatggtaac
    24901 acgttcaata taataatttt gcattatcat tgctctcctt taaattcctt tgcgcgttgg
    24961 tttatttgtc gcagaagctc aacgtcactt ttatcttgaa tgtatttaac agattcgtca
    25021 gctatttgta agtatttttc tgcatatacc gatacgaatt gtgatatcaa ctttaggcca
    25081 tcttctgata aagcatactt aaaaccttct ttgctttgcg atggttggat aacatgttgc
    25141 agtacaagat ttttgactgc atctttaact aattcacgtc gcgcaataaa ttcactgaat
    25201 ctatagctgt tatcaccatt taagttgtaa tcggaaattt caaagtcgcg accatacgtg
    25261 gtgatgaagt ctgtgattaa aattctatca gcgctgacag gctctttaaa ttcagagagc
    25321 agtaacatta ctcgaaccga aatttcaaat ggagtattaa acaaactatt catcttcagt
    25381 cacccatttg atctttccgt cattaaccag catgtggcaa atgccttttt tagcagaatt
    25441 ttgcagcaat cccggtataa acgataagat tgatccgttt aatgttataa cagacgactg
    25501 ctgtagaacg gctttgagtc tctcaacgcc atcatcgtag tcttcttcat agacttcctc
    25561 gatgccgtcg tacatatctt cttttaaagc ttcaaagtgc tcagttcctt cctcttgtgt
    25621 aaagttatca cggaccccac gacgtatgct ttcagcttta tagtaatttc ttcgctgtct
    25681 cttgaaatcc tttagccgcg aatttggcag gtcgtttgta tgctcgtata cttcgtatcc
    25741 atcggcatct gcatatgctg aaaaaagagc tatgacatac gtcatctcct cagctctaat
    25801 atcaagggga actggcaaat cgtcaaagaa ttttttcact tcatcattat aagagaaatc
    25861 accattagtg ttgattctta aacttgcgag ctctaattcg ctaagcttcg gcttggcctt
    25921 ttttgttttt acttttgttt gagaagcaat tgaggtgatt tcatcaagta aaagatgggc
    25981 cactatttca gaaacgtctg tatctttggg gattaccaca cctttatcaa tcaattgttg
    26041 cttaaggacg gttggagcat catctataag acgttcataa ataaaagtcg tgcatttgtc
    26101 gatgttgaat tttccagcaa cccgaactgc atactcttgt gttaatggtc gagtaccatt
    26161 gtaaaatctt cgtaggttat catcagaaaa accgaaaatg gtggcctcgt catcatttga
    26221 aacactatcc agtaaagcaa ttaaaaaatc ctgtggcttc acgtactcgc ttcccataaa
    26281 cggataaagc gtttgagtaa tttcacttat ttccaaaata caacctcctt tgttacgtcc
    26341 gccgttgacc gccgtttgcc gccgatgact gacgaaaaca agggaggcct ttttctataa
    26401 ttaaaacata gtagtttaac tatttaattt tatcatttat ggttcataaa cactacatta
    26461 tgtttcaaga aagtgaacga atttatctta aaagcgaata gtcctactaa taaaatataa
    26521 caatgcctga gttgcaatag ggcaaggtta caaatactga aaagaactgg ttatccagaa
    26581 aatttttcag atattgtatg ccttacttta ttgcgctctt ttttcgtgat gagggacggt
    26641 gtacgtattg gcacggtccc tgtttgtttt ccttgctcaa aagctctttc aggcaagaaa
    26701 gagggatcta atgagtagat ttatgaaaac acaccaaaac gaccgcagca cttacaagta
    26761 cgtgtttgag cgtactgatg ccaatggacg tatccaaaaa gaagtggttg agattcgccc
    26821 aggcgaagac ggcgtcactg agcttaacat tcaggagctg catcgcgctg acgaccgtgc
    26881 gatttacaac ttttataaaa acgcacgtcc tgaacgcaca gaagaagaaa aagaaaagat
    26941 caaggcttgg aaagagaagt tccgtgaaga ctttaataat gagcacggct attatccaat
    27001 ggacgaatac gtggatggtg aagccaacga ttacttcaaa cgtaactaca atttgtcgct
    27061 caatgaattt gatgatgaat gcgatgaggg ggacaacccc atccaatcat tggtttacga
    27121 tgcttaccaa gataatgaag aagatcctcg tatcgatcgt ttgcgtgaag tcgtgacgca
    27181 attattaccg taccaccaag aaatctatca catgctcgtc actgaaaaca tgtctcagac
    27241 tgaagtggcg gacaagcttg acaagaaaaa acagtcaatc aatcgcacct ttggaatcat
    27301 caaagacaag atcaaagaat tattttagaa aaaagttacg ggggagttta ctttccccgt
    27361 tttccttcgc ctgtattgtg taagggagta gatcctaaac aaataaagat gaaggaggac
    27421 catcatgaac ctaaaacaca aaatccgagt aaacgtgact gatgacacgg gtaacaaaga
    27481 aactgtccta caaggtggtg tgcgtcattt gcctcgctgg tttgcacggt ggctgttcgg
    27541 taagggcaac atggacaaaa ttctcgtgct cacaccaggg gacacaatcg aatcagtcga
    27601 aatccgtgaa gttaacaaga agggagcgta atgacgtgag taaaacaaaa ctatttcttc
    27661 aggtcgttga ggacgtccaa gcgttagctg atagcttaca agagttggct gtggcgatga
    27721 aaaccaatga acctgaacca caagttgaaa caccagaacc gacaatggaa acaaaatcag
    27781 aagtcaaaca accgacgctt gaagaagtcc gtggtttgtt ggcacgtaaa tctcaagaag
    27841 gtaagtcaac tgaagtgaaa gcactgattg agaaatatgg tgccagtcgc ttgagtgatg
    27901 tgccgacaga aaactacgcg gctttggttg cagatgcgga gggattgtag atgccaacac
    27961 aacacgcagt cctatccgca tcttcaagta atcggtggat tcactgtccg ccaagtgttc
    28021 gcttgtcaga gcactttgaa aacaagtcga gcccgtatgc ccaacaaggc actgatgctc
    28081 ataccttatg tgagtacaaa ctgaataagt tcattggtaa tgacgtcaca gacccaacat
    28141 ctacgcttga attctacgac gaggagatgg agcagtgtgc tgaaagttat gcgaccttcg
    28201 tcatggaaga agtcgctaag gcgaaacaga caacagttga ctctattgta atagtagaac
    28261 agcggctcga cttctcaagg tacgtcccag acggctttgg gaccggcgac tgtctcgtca
    28321 ttgcggatgg aaccctatct gttattgaca tgaaatacgg tctaggaatt ttggtagatg
    28381 cgtatcagaa cccacagatg atgtgttatg ccttgggagc gttggagctt ttcgatgtta
    28441 tttatgacat tcaagaagtc aagatgacca ttttccagcc aaggcgtgag aatgtgagta
    28501 cgtacacact gctgaaagaa gaattacttc agtgggcaaa agatgtgttg tcgcctgccg
    28561 cagatttagc tttcaaaggt gagggtgagt accaatgcgg gaaatggtgt ggattctgtc
    28621 cggcgaagaa tagctgtcgt gcacgagcag accataactt gaaactggcg caatatgagt
    28681 tcaaaccacc agagcttctt agtgatgatg aaatcgaaga aatcatcggt aaagtggatg
    28741 acctggtttc ttggagtaac gatatcaagg actgtgcttt gaaacttgca ctcggtggca
    28801 aacaatggac tcatcacaaa ttagtcgagg gtcgctctac tcgtaagtat tctaatgaca
    28861 atgatgtcgc tgcggcggtc atcaaggctg gatacgaccc atatgataaa aagttacttg
    28921 gtgtcacagc aatgacgaag gcactcggta aagagaaatt caatgaaatc ttgggcgaat
    28981 acatcatcaa accaaaaggc aagctgacgc tagtcgatgg ttcagataaa cgacaagcgg
    29041 tcacagtaaa caacttaaac gaagaattta aaccattaac ggaggaacaa taatatgtct
    29101 aaaacaagca aagtaatcac tggtacaaac acacgtctct catacttcca cggttgggaa
    29161 ccagcatcca tcaacggggg cgcagagaaa tattcaacag cagtcttgat tccaaaatca
    29221 gataagagaa cgattgatac cattgaaaaa gcaatcgatg cagcaatcga agagggactt
    29281 gcgaaattcg gtgggaagaa accaaacaaa gcagaaatca agctgccatt acgtgatggg
    29341 gatatcgaac gcgacgatga agcataccaa ggccattact tcattaatgc gaactcaacc
    29401 actgcaccac aaatcgtgga tcagcacgtt caaccaatct tggatcgttc acaagtgtat
    29461 agtggttgct acgctcgtgt gtcgattaat ttctatgcct ttaacgcaaa tggaaatcgc
    29521 ggggtagcgg cagggcttgg gaatatccag tttgtacgtg acggcgaacc actcggctca
    29581 caagtatctg caagcgatga gtttgaaacc ttggcggatg atgatttctt ggcttaatca
    29641 ttaaaacaaa aacggccatg gcagaagtgc tgtcgcctgt ttgattggag gaagaaaatg
    29701 aaacatttgt cattggattt ggaaacgtat agtagtgtcg atttatcgaa atgtggtgtc
    29761 tacaaatatt cagagtcccc cgattttgaa atactgctat ttgcatacag tgtagatggt
    29821 ggtcccgtcg aagtggttga tctgaagatg gacgaaacta tcccggctga aatattaact
    29881 tcgctatcag atgaaacagt gattaaacac gccttcaatg cgagttttga acgtgtgtgt
    29941 ttgtcacgct ttctggggtt ggatggcttt ttgaatccac gagggtggca atgcacaatg
    30001 gtgtggggtg cgacattagg tttgccgttg tcacttgtag gagttggtgc tgttcttggt
    30061 ttggataaac aaaagctcac agaaggaaaa gacctcatcc gatacttttg cgtgccatgc
    30121 aatccgacca aagttaatgg tggacgcacg aggaacttac cacaccatgc gccaactaaa
    30181 tggtcagcat ttaagacata caatagtcgt gatgtggaag ttgaaatagc tatccaagga
    30241 aaactttcca attttccagt gcttgattct gtttggaaag aatactggct ggatcaggaa
    30301 atcaacgacc gaggaattga gattgataag gactttgtgg aagctgccgt tcagttggat
    30361 gaattatcaa aagctggatt gaatcaacaa ttgcaagaat taactggact agaaaatcca
    30421 aacagcgtgc ttcaaatgcg gggatggtta ctagaccacg gacttgaaac cgagtcactc
    30481 ggtaaaaagg cagtggccga actactcgaa caacctattg atgaaaattt aaagcaagtc
    30541 ctggtcttac gtcagcaact cgccaagtca tccgtgaaaa agtatcaagc catgcaaaac
    30601 tttgcgtgtt ctgataatcg ttctcgtggt ttattccaat tctatggagc caaccgtacc
    30661 ggacgctttg caggacgtgg catccagctt caaaacttgc ctcagaacca tatgtcagat
    30721 ttatctgaag cgcgtagttt agtaaaacaa agcaattttg aaatgcttaa cattctctac
    30781 ggcaatgtcc cgaacgtact ctcagaactc ctccggacag cgtttgtacc acgtgaaggg
    30841 atgaaattta tcgtatctga cttctcagcg attgaagcac gtgtgattgc ttggctgtcg
    30901 ggtgagaagt ggcgtcaaga agtattcaaa aatggtggcg atatttactg tgcgtctgcc
    30961 agtcagatgt tcggtgtgcc agttgaaaag catggcgtga atgggcatct acgtcaaaaa
    31021 gggaaaattg ctgagcttgc tctagggtac ggaggatcgg tgggtgcatt gaaagcaatg
    31081 gatgctcttg aaatgggact aactgaggat gaattacagc cgcttgttaa ttcttggcga
    31141 gactcaaaca ttaacataac ggagctttgg tgggctgttg ataaggcagt aaagacttgt
    31201 gtgaaagaac gacggagcac agcgacgcat ggcatcaagt ttgaatacaa gtcgggtttt
    31261 ctgttcatcc aactcttgtc aggacgtaaa ttagtttatg taaaaccaaa gattggtgag
    31321 aacaaattcg gcggtgaagc tgttacctat gaaggtgtcg gtgctaccaa gaaatgggaa
    31381 cgtcttgaaa gctatggtcc caagtttgtc gaaaacatcg tgcaggcaat agctagagac
    31441 attctctgtt tcgcaatgga gaatcttcgt aagtatcgca ttgtcgggca cgtccacgac
    31501 gaagtgatta tcgaagtgcc aatggaatcg aaggttgaag acatctgtca gttgatgagt
    31561 caaacgccta agtgggctga aggcttgcta ctgaacgctg atggatacga gtgtgaattc
    31621 tatcaaaagg attagtcaat gtggagtcag aaatggctcc atattttttt caaatttttt
    31681 tatcaaaaat aaggttcgaa ttgggttctt tcttcgctta tagacgaagg caaaaaaatc
    31741 aaaaataaat tcgagtcaga gtttactttc gaccattttc ttcgcctgta agttagaagg
    31801 ggcaaaatgc ctctcctgaa aattctatta caggaggttc acaagatgaa cgaattgaaa
    31861 gtttttgaaa atgcggagtt tggctctgta cgcacggtga tgattggcga tgtccctcac
    31921 tttgttggta aagatgtagc atcaattctt ggctacacaa atccacaaaa agcattacga
    31981 gatcatgttg atgaggaaga taagacggtg aacgaatcgt tcagcgttaa tgggacaatg
    32041 gctatcctta tcaacgaaag tggattgtac tcactcatca tttctagcaa gatgccgaac
    32101 gcgaagaaat tcaagcgttg ggtgacaaat gaggtcttgc cgtctatccg ccaacacggt
    32161 gtgtacgcga cagaagattt catcacaaag tcaatcgaag atccagcatg ggccatttca
    32221 gtgttgaagc agctacaaga gaaaaaagaa atggtggcga tgcaacaaca aatgattttg
    32281 gagatgaaac caaaagttag ctactacgat ttgattcttc aaaacaattc tgtgatgtcg
    32341 atttctaaaa tctcgaagga ctacggtatg tcaccacagg caatgaacaa gttgcttcat
    32401 gaactgggaa ttcagtacaa gcaaggcaag atgtggttgc tttatcagaa gtatgccaat
    32461 caaggctaca cacaatcaaa aactcatgcg atagatgcag agcgcagcgt gatgcatacc
    32521 tactggacgc agaaaggtcg tttgttcttg tacgacttgc tgaaatcgaa gaagaacatc
    32581 ttaccagtga ttgagcgagg tgataataat gcctagacgt aaagattaca gaacatcagt
    32641 ctatgtctgc tcgccgttct caggaaatgt tgtcgtcaac cataaaaaca ctaagcgtta
    32701 ctgccgtatt gctcttgacc gtggctacat gccagttgcg gcacatctga tgtatccaca
    32761 attcatggac gacgcaacgg agcgtgatgt ggcaatgcac atgtcacttg ttctcatggg
    32821 caaatgtgag gaagtctggg tggtgggtga caccatctcc caaggcatgg cagttgaaat
    32881 tgaacaagcg gagtattgga agaaacatat caggtacttc gatgatgaga tgaaggaggt
    32941 ctcaaatgct taagatgacg ttattcacat ccaatcaggt gggtaattcc aagaatggat
    33001 tgttcccaac caagcaagtg gtgacagata aagaaacact tgttgccacg gtcaagctgg
    33061 atcatgtgat ggctgagtac aaaggcaact atcgcaaagg tgacaacttc atccagtctg
    33121 atgtgattcc acttgattgc gacaatgacc actcagacaa tccagatgat tgggtagccc
    33181 ctttcgaagt ggcgatgcag tttcctgatg tgacgtttgc ggtcagctat tcacgtaaca
    33241 acatgaaaga caaaggtggc aaatcagcac gtccacgttt tcacgtatat ttcccgattc
    33301 ctgccacaac tgataaagat gaatatgtgg aactcaagaa gcagattcaa agtgagttcc
    33361 catactttga caacaatgcg atggatgcgg cacgtttgct tttcggaaca ccaaactctg
    33421 aagttgaaat ctatgaaggt agtaagttga tcactgattt tctcgaagat gatctgtttg
    33481 ctgattggga taatgcgcag tcagagatta gcgaaggcag ccgtaattca agcatgagtc
    33541 acatcgcagg aaagctcatc aaacgctacg gtgcaacgga agatacgtat cacaagtatc
    33601 tcgaacaatc ccaaaaatgt aatccgccac tgccggagga ggagcttaaa gctatctgga
    33661 atagcgcggt caactttggg aagaaagtga aacaacaaga aggctacatt cctccagagc
    33721 agtacaactc tgatttatca cttgagccaa ccgactactc ggacttaggt caagcaacgg
    33781 tcttagctcg cgagtatgaa tgtaagcttc attactcacc gtcaactggt taccttgtct
    33841 acaacggcag ctattgggaa gagtcgaaac caaaagcgca aggtgtgatc cacgcattga
    33901 cagaacgtca actggaagaa tctgaaaccg aaattgagaa gcgtaccaag gaaatggtta
    33961 gtaatggcgc gttcgatgta cttgctagtg ttggtccgaa aaaggcagtc accatgttca
    34021 acacagcgca acgtcatagt tttgatttgt accaacacgc acaagagtat aagaagtttg
    34081 ctgtcaaacg ccgcgacagc aagtatctct cgtcagcatt aaccgaggca aagcccatgc
    34141 ttgaaatcga gcaacggtta ctagatgtga acgagttctt gctgaacaca ccaacagcaa
    34201 cctttgattt gcgaacaggc aaatcacaag accacaactc agaagattac atcacgaagc
    34261 aaacggaatg tgccccgagt gacgccaatc agcagatttg gttggacgct ctgaacacca
    34321 ttttcgtagg tgaccaagag ttgattgatt atgtccagat gattgtagga cttgcagcta
    34381 ttggtaaagt ttacgttgaa gcgcttatca tctcgtatgg tgaagggcgt aacgggaagt
    34441 ccacgttctg gaatgtcatc agccgtgtgc tcggtaatta ctctggcagc atctcagctg
    34501 atattcttac aagtcaaatc cgacgcaatg tgaaacctga acttgcggaa gcgaagggga
    34561 aacgcctact catcgcagcc gagcttgaag aggggatgcg actcaacaca tctaacatta
    34621 agcagctctg ctcgacggat gaaattgcgg ctgagaagaa atacaaagac ccattcaagt
    34681 atgtcccaac gcatactttg gtgctctata ccaaccacct cccaaaagtt ggagcgattg
    34741 ataaaggaac ctggcgtcgt ttaattgtga ttccattcct tgctacgatt gacggcaata
    34801 aggacatcaa gaattatgcg gactaccttt ttgaaaatgc cggcggagcc attttgcaat
    34861 ggatccttga cggtgcgaaa aaagtcatcg ccgcagactt tcatttaacg gttccacaag
    34921 ttgtcgctaa tgcaatttct gaatataaga cggcgaatga ttggcttggc catttcttag
    34981 acgagtgctg cgagactgct gttgactttg aagaaaaatc tggtgagttg tatgcagagt
    35041 atcgtgcgtt ttgtacgcga actggtgagt acattcgtag ttcagccgat ttttacacgg
    35101 ctctggaggc gggcggattt aagcgccggc gaactaataa aggaaacaag attgttggtt
    35161 tgcaactcaa atccgagttc ttggaatagt tgattttaaa ggaaagtgta acctgtgtag
    35221 gtcttatata gaacttttct taagggataa aaaattacca cctatataaa gttatgtatt
    35281 gcgtattcac gggttacacc atacttgcag attggagaat atctaaatga aatttaaaca
    35341 gtggttacaa attggaatcg gtaaacgtga tttacgattt gatgagtttt acgagtttgc
    35401 catgtcggat gtcacgtatc cttggaagaa atcattcgat aagcaagtcg cacatctgat
    35461 gattcactgc gcagaaaagg aatacttgga aatcttgcgt gatgcgtatt tcgagtattt
    35521 gacggtgtgg ctatgagaga aaagcaggtt gagcaagcac tagtgaaagc cgtcaaaagg
    35581 gtaggtggta tttgcccgaa gtttacatca ccggggcttg ctggtgtgcc tgaccgattg
    35641 gtacttatgc caaatgggaa acttggattt gtcgaagtga aggcgccagg gaagaagcca
    35701 cggtctttac agctttttcg tatgaaacaa ctgacagatt tagggtttca gtgtttcgtg
    35761 cttgatgaga ttgatcagat acctgagtta ctagaaagaa taggaggtga tgtctgatga
    35821 agttcgtacc acataactac cagaaatatg cgattgattt tatcaaagat cataaaactg
    35881 ccgcccttct attggacatg ggcttgggta aaacggtgac aacgctgaca gctattaaag
    35941 acttgatgca tgatgacttt acaatacaac gtgtcttaat tattgcaccg ttgcgcgtga
    36001 cgcaatcaac atggccaact gaaattcaaa agtgggatca tttgaaagat ttatcgtatt
    36061 ccgtggttct tggaacacca agtcagcgaa tggcagcatt acaaaataat gccaacatct
    36121 atctgatcaa tcgtgagaac ttggactggc tgattaacaa atcgggtttt acgtttgatt
    36181 ttgatatggt ggtgattgac gaactgtcgt ccttcaaaaa cttaaaagct aaacggttca
    36241 ctagctttat gaaagtcaga cacaaggttg accgaattgt tggattgacc ggaacacctt
    36301 caagtaatgg tttgatggat ttgtttgcgg agttcaaggt tctggatatg ggcgagagat
    36361 tgggctatta tatttcacgt tatcgagata aatactttct acctgacaag caaaatggca
    36421 tgcagattta ttcttggaaa ccaagggaag atgcagaaca agaaatctac gacaagattt
    36481 cagatatcac gatttcaatg aagtcagtcg atttcttgga tatgccagag cttgtgatga
    36541 atgaagtgcc agttgatatg ggactcaaag aaaagcaaaa gtacgatgaa attaaggcag
    36601 acttggtgct acaattgaaa gaagccacca ttgatgcagc caatacagcg gtgctttcga
    36661 ataaactgct acaaatggcg aatggagcaa tctatgatga gtttaatgtg agtcatcaca
    36721 tccatgacca gaagttagat gctttggaag atttgattga aggtacaaat ggcaaaccga
    36781 ttctgattgc ctattggttc cagcatgatc tggagcgtat caaggaacga ttcaacgttc
    36841 gacagatcaa aacgtctcag gacattattg attggaacga aggtagcatc cccatcgctg
    36901 tcattcatcc ggctagtgca ggacatggat tgaatttaca agctggtggt tcaacacttg
    36961 tatggtttgg tcttacgtgg agtttggaac tttaccaaca aaccaatgca aggctgtggc
    37021 gacaagggca aagtgatact gtggtcattc atcacattat tgcgaaagat acgattgatg
    37081 aagatgtgat gttggctttg aaacgaaaag ataaaacaca atcatgtttg attgatgcag
    37141 tgaaagctag attggaggga agcaagtgac accaaaagaa tacttgaaac aagcgtaccg
    37201 tttgaataaa cgaattgacg accggattaa agagaaggcg gagctctatc aactggcggt
    37261 gacagtttct tccacaacat tcgaagaaaa gatttcaagt ggcacacgga atactgaggc
    37321 accatttgtt cgtaaccttg agaagtgtta ccaattggaa gctgaaattg atgcagagat
    37381 tgataagttt gtagacctca aacgacaaat gcgtggcgtg attgatgaac tcacaaacgt
    37441 ggacgaaaag attgtgttaa tcaatcgata cctgttggaa gagagctggg acaagattgc
    37501 tgaagatctt cacgctgacg tgcggacagt ctttcgttgg catagcaaag ctcttgagaa
    37561 cttgaagcta ccagatgaac ccatagtcat ttgaaatgtg tcagtgaatg tcagtagcct
    37621 ttagtgatat cattaagatg tcaaaaaaga caaacgatac tcaagcaata ccagcaatag
    37681 aagtatcgaa caaatactcg aagcctttgt gggaaaaatc cttcaagggc ttttcttttg
    37741 cccgaaagga tgtgacccca cttgccaatg aaaccagcta acccctgtaa gttcccaggt
    37801 tgtccgaacc gtacgcatga tacttattgt gatgaacata aaacacttgc gaggaaacgt
    37861 tacgagaaat acgagcgtga tccggcaatc aacaaacgtt acggcagagc gtggaagaaa
    37921 atacgggcaa ggtacgtggc agcacatccc ctatgtgaga tgtgtctgaa ggaaggacgc
    37981 tttacttcgg ttgagattgt gcatcacatc aaggaattaa gtgatggtgg tacgcatgat
    38041 gaaaataatt tgatgagtgt ctgcaaatca tgtcactcga aaattcacat gactcgacga
    38101 aacacaaaag ataaaacaat catgtgaaaa ataacaaggg ggggaggggc ggtaattatc
    38161 cctaaaccct tctaaactgt acagcgcgct aggcccttac gcgaaaaaag ttcagttcaa
    38221 acgggggata gccccaaaca agaaaaagag gtgataaatt ttggcaaaag atggaacaca
    38281 acgcggtggc agacgacctc gttcagggga acgctcacag ccactcgttg ataaagttgc
    38341 tgctggaaag aaagcatcgc ggatggaatt tgacttaccg gatgttgaat tattaattgg
    38401 tgaagatatt ggtgagggtg tagagcttga gggcaatgat tatcctgaac caagtgctta
    38461 cctttctgca aaacagaagg atggcactgt gctaggcgct gatgaaattt ataactcaac
    38521 gttccaatgg ttgaaagacc gcggatgcga aacgttgata gccccacgaa tggttgagtc
    38581 ctacgctcag aactttgcgc gttttgttca atgtgaaaat gctctgagtc aatatggttt
    38641 gctcggaaaa cacccgacta ccaaggcagt catggcctcc ccatttgtgg cgatgtcgca
    38701 gaacttccaa aagcaagcaa atgtgacgtg gtatgagatt caacagattg taaaagagaa
    38761 tgcatcagtg gaatacacag gagatccgaa tacggatgcg atggaacgat tgttacgttc
    38821 taagaatggt tgaaaggaac agaatttatg atagagaagg ttaatccgca acatccagat
    38881 aaagttgcag atcgtattgc cggagccatt gtggacttgg catacgcaga agaaaataat
    38941 ccaaagatag cagttgaggt tttaattggt catggcatct gtcatgtgat tattgaaact
    39001 tcaactgctt taaatttgaa tgaaatcagc caagcgatta atcgtatcgc aggcaaaatt
    39061 aaaaaagatg tggtgattgt gccccaagat aaagagttgg ctgacaatca agcgactgaa
    39121 attcgttgtg gcgataatgg aatctttcgt gggatgccat tgactgatga acagttacag
    39181 ctctcaaaaa ttgcgcgtga cttatatcag gaccatccat ttgatggaaa gtacattctt
    39241 gatggtgaac gcttgataat ttgtcagagc cacgcaaaga caaatgagtt aaaacaaaaa
    39301 tatccaaacg ctacagtcaa ccctctaggt gattggactg gcggcccgga tgtggataca
    39361 ggtgcgacta atcgcaagct tggttccgat atggcagacg gagtaacagg tggcggtatc
    39421 catgggaaag acttatcaaa agctgatgtg tcagtgaaca tctacgcatt cttgaaagct
    39481 caagaaactg gtatgcccgt tgagttaagc tgtgcgattg gtgacactga gattgatggc
    39541 gtgccttatt ctgaaatcgt ggaagttgcg cgtgattatg tgaatgagct tggcgggttt
    39601 gagaaactag cagaatgggg gttgttctaa tgaacacaac agaacgtttt gagaaagttg
    39661 atattgataa gctggtccct tatgccagaa atgcccgtac ccacagtaag gatcaagtga
    39721 aacagattca agcttctatg cgtgagtttg ggttcataaa tccggtgatt gttgataagg
    39781 attataactt gattgcaggt cacggacgta ttcttgcggc taaagaagaa ggtatcaagc
    39841 aggtaccgtg cgtgtttgtg gaacacctaa ccgaggcaca aaagcgtgcg tacatacttg
    39901 ctgataaccg actagcaatg aacgccggtt gggatgatga aatgttggcg gttgagttat
    39961 cagacctgca aggtgaagag tttgatttgg atttgcttgg ttttgaaact gatgaacttg
    40021 ataaattact caacggtaat gatgatgccc aagatgatga ctttgatgta gatgaggaac
    40081 ttgaaaagcc agccttcagt aaaacagggg acttgtggtt gcttggtcgt caccgtttag
    40141 tttgtggtga ctcaacaaaa cctgaaactt atgagaaact catggacggc aaactagcaa
    40201 atcttgtggt gacagatcca ccttatgggg tctcatatca aggtaaagcg ggaacgatta
    40261 aaaatgataa tctgaaggac gaggcatttt acaacttctt gtatgatgcc ttttcttgta
    40321 tggaaagtgt gatggccaac gacgccagta tttatgtttt ccatgcggat acggaaggct
    40381 tgaacttcag aaaagcattc gtggatgcag ggttctattt atcaggtacg tgcatctgga
    40441 agaaacagag tctagtgctt ggtcgttcgc cttaccaatg gcagcatgag ccggtgctct
    40501 atggctggaa gaagaaaggc aaacatcaat ggtacaccgg acgtaaggaa tcgaccatct
    40561 gggaatttga taaaccaaag aagaatggtg accacccaac gatgaaacca attccgctgt
    40621 tggcatatcc gattaccaat tcgtcactca gcaactgcat cgtgttggat agttttggtg
    40681 gcagcggatc aactttgatt gcttgcgaac agaccgaccg gatttgccat acgatcgaac
    40741 tggatgaaaa gttctgtgat gtgattgtta agcgttatat cgagcaggct ggaagcgatg
    40801 cagacgtttc agtgattcga gatggaaaga ctatcgcatt taatgaccta gaaaatcctg
    40861 aataaataac gtatcacttg ctataaaatc cctttagagt gatatataga ctaccaaaag
    40921 aatacggaag acaaagttat gtttccaact catgaacaaa tcgagggtat gaaaaaacgt
    40981 taccctgaag gtagtcgtgt agaactcatc aagatgaacg acccacatgc cccagcgaag
    41041 ggcacacaag gcacagtaac cggtgttgac gacattggtt caatccttat ccaatgggac
    41101 aacggttcaa cactgaacgc ggtatacggc gaagatgtgg tgcacatcat caaaccagac
    41161 ttcgaattgg tctttcaaaa cggtagaaga gaaggatttg aaaccttcaa tgatgcttgg
    41221 caatacgtga cggacatgat attgaaccac gatttggtct gggttgacct tgagtcaaaa
    41281 acagaagata ccatctacat cagaaagggg ctttaagatg aaagtgagta aacgtaagat
    41341 ttacaacatc gcaaagaatt acatcgttca gttgcctgaa cgtggcgacc tgaaggccca
    41401 caacagcgac cgtgaagact tcttggacat tgcggtttgg tgtttggaag atgccttaat
    41461 tgcggcctat gaggcaggca gaaaggacgg tcaaaatgaa cgcaaagatt aagcaacaaa
    41521 tcattgagat tgcagaaagt ggtctgacaa atatgtttga tgcgattgca gtgcagcgaa
    41581 ttgcacatga ccggcacttt tacgagttgg tgattttcat tgaagaacac aaagatgagt
    41641 acagccgctt tatcttggag ggtgataaat gatggattat caaatttaca tgccagataa
    41701 tgaagatggc attgaagaag gatactacga ttggcaagaa ctggtgcaat tgctgcggga
    41761 taacaagaac aaccctgaag ccattcagtt cattgccgat atgatggaat aaagagatta
    41821 aggcagctga caacagctgc tttttgcgta gaagggagtg agttccaatc gataattaca
    41881 aaccaactga atttatggac aaagactccc attatgataa gtcacgtgca gattatgccg
    41941 tggcctttat tgagttgttg aaacacacga aaggtcagtg gttcgggaaa ccgttcaaac
    42001 tgatgccttg gcaagaaaag attatccgtg acttattcgg aacgattaag gcaaatggct
    42061 acagacaatt caatacggct tatgttgaaa tacctaagaa gaatggtaag agtgagttag
    42121 cggctgccgt ggctttgctt ttgacttgcg gtgatgggga agaacgcgct gaggtatatg
    42181 gctgtgctgc tgatcgagcg caggcttcaa tcgtatttga cgtagctgtt gatatgattc
    42241 gccagaaccc agcgttaagc aaacgagtga aaatcctgac ttcacaaaaa cgaattattt
    42301 atcagccaac gaatagtttc tatcaagtat tatcagctga agcatactct aaacacggat
    42361 taaacagttc tgcggttgta tttgatgagc ttcacgcaca acctaacaga aagctgtttg
    42421 atgtcatgac gaaaggggca ggggatgcta gaacgcaacc gctattcttt ttgattacaa
    42481 cggcagggaa tgataccaat tcaatttgtt atgagcagca tcaaaaagct ctcgacatta
    42541 ttgaaggtcg aaagcatgat aaaacattct atccgttaat ttacggtatc aagcaagatg
    42601 atgattggac tgatccagaa gtttggaaga aagccaatcc gtcacttggg ataacgattg
    42661 cagaagaaaa ggtggtggct gcttgtgatt cggctaaaca aaatccagct gaagaaaact
    42721 cattcagaca gcttcgcctg aaccaatggg tgaaacaagc agtgcgttgg atgccaatgg
    42781 ataaatggga cgcttgttca ttcaatgcag atgaagaaag tttgagaggt cgagtttgtt
    42841 acggaggtct cgacctttcc agcacgaccg accttacatc cttcgttttg gtgtttccac
    42901 ctgaagatga ggatgacaag tttgtgatac tgccatatta ctgggtgcca gaagatacct
    42961 tggacttacg agttaaacgc gaccatgtga attatgattt gtgggccaag caggggtaca
    43021 tacaaaccac tgagggaaac gtagtccact atggtttcat tgaaaaattt attgaggaac
    43081 tgggtgagaa atacaatatc cgggagattg cctttgaccg ttggggagca gttcaaatgg
    43141 ttcaaaactt agaaggtatg ggatttactg tagttccttt cggtcaaggt ttcaaggata
    43201 tgagcccggc tactaaagag ttgatgaagt tgactcttga acagaaatta gcccatggcg
    43261 gtcatccggt tcttcgttgg aatatggaca acattttcgt ccgaactgac ccggcaggta
    43321 atatcaaagc tgataaagaa aaatccactg agaagattga tggtgccatc gcgacaatta
    43381 tggctctgga tcgtgcaatt cgttgtggaa atgataatgg tgccagtgtt tatgatgata
    43441 ggggattgtt gtttctgtga aatatgagct tttattacaa tccattgata taattgtctt
    43501 tggaggtgta tgtgtatgaa tgaatatttg attcaagtag caccagataa aatatcttca
    43561 gacccggaca acacattaat ttctagactg ttttatagaa aatggtgggg aaaaattcta
    43621 actgaagatt tgacaaagta tgctgaagga attgggaatt ataggggaga aacgattatt
    43681 tctttcaaca caattgctgg tgcaacgata cggttattga aatgctataa tcaaaagaac
    43741 ttttcaatgc cagatagtca ttatgggcgt ttgaaagtca ttttatattc cgatgatatc
    43801 accgaagaga tgaaagtgaa atttgtaaaa ttctatgatt taagtcatac tcttgcaaat
    43861 tttatgccac tatatgtatc aaaaccttct ctcaatggtg ttaaaggtaa agggccgtat
    43921 catgactttc ctgatttatt ttattctgat gtaagagata aacgatatgg ttcatcaact
    43981 aacgctgata aatttatcgt taataataaa gactatttct ctaaattcaa agattggaaa
    44041 agttttatag aactaaatta tttacaagat ttcttcgatg atgaagatta tatgacgttt
    44101 aaaaaacttg caccaagtag ttcattcttt ccttatcaaa gaaaatcctt aacacctgac
    44161 gaaaaatcga aatgtttaga ggaagtaaat acctttttaa ataatgcaat tgagataatt
    44221 ttgtcaagag gaagtcggtt ttatatctaa tgtgaacaca gcatctacaa atcgtaggtg
    44281 cttttttgat actcaaattt tgaaaggagc aatcaatggg cataataaaa tcactattca
    44341 aatcacgcga taaaccaaca aatcaaacca tcatgaatcc ataccgattt atgtttggcg
    44401 gctcatcagc tggtaaaagc gtgaccgaac aaacagctat gcagatgacg gctgtgtact
    44461 catgtgtgcg cgtgctatca gaagccgtgg caggattgcc attacacttt taccaatacc
    44521 gagatgctgg cggcaaagat aaagcaatga aacattcgtt gtatttcttg ctgcatgatg
    44581 aaccaaattc ggaaatgaca tcatttgtat ttcgtgaaac cttaatgact cacctgctac
    44641 tttggggtaa cgcctatgcg caaattatcc gaaatggcaa aggtgaagtt gtagctttgt
    44701 atcctttaat gccagataag atgacggtta atcgcgatga caacaaggaa atctattatt
    44761 tatatcaagc tgatgtcggg caagtcagac tttcaaaagc tgacgtgctt cacattcctg
    44821 gccttggctt tgatggcttg gttggttatt caccaattgc catggctaag aatgctatcg
    44881 ggcttgcgat tgcaacagag gaatatggtt ctaaattttt tgccaatggt gccaatcctg
    44941 gtggtgtcct agaacatcct ggaactttga aagacccagg aaagcttcgt gaaagttgga
    45001 atgcgacatt tggtggcagt tcaaatgcgg ccaaggttgc agtacttgaa gaaggcatga
    45061 agtatacgcc tatttccatg tcaccagaac aagcccagtt ccttgaaaca cgtaagtttc
    45121 aactggaaga aatcgcacgg atattccgag tgccccttca catggttggc gacttagacc
    45181 acgcgacctt ctcaaacgtt gaacatttga gtttggattt tgtgaagttc tcattatcac
    45241 catggctcaa tcgttgggaa caagcactta ataaatcact gttaagtcgc gatgaaaaag
    45301 aaaagtattt cataaagttt aatgtggatg gcttattgcg tggtgactat gccacacgtc
    45361 aacaaggcta tgcaaccggt attcaaaatg ggttcctatc tcctgatgat gtgcgtgaac
    45421 ttgaggactt gaacccaatc cctggtggtg ctggtgcgaa atattacttc aatggcaata
    45481 tgctgccaat tgaacaagcc ggggcagctt atggcgtaaa caaagaagaa agcgaggaac
    45541 aagatgaaga agttttggaa gtggaagaat caaacggttc tgaatcaaga caaccaagag
    45601 gaaatcctag agagaaccct ctacctcaac ggaccaatcg ctgaggagag ctggtttgat
    45661 gatgaagtca caccgcagat tttcaaagat gagttaaatt caggtggagg taacgttaca
    45721 gtctggatta attcgggtgg tggcgattgt gtcgctgccg caagtattta caacatgctt
    45781 gctgactaca aaggtaaagt cacagtcaag attgacggca tcgccgcttc ggccgcaagt
    45841 gttattgcaa tggcaggtga caaggtactc atgagtcctg tggcgatggt gatgattcac
    45901 aatccgtcag cggttgcgtt tggcgaaaaa gttgatatgg agaaagccat tgccatgctt
    45961 gatgaagtga aagcaagtat tatcaacgcc tatgaacgta aaacgaattt atcgcgtgtg
    46021 caactggcac atatgatgga tgcggagacg tggatggacg caaataaagc tgttcaccta
    46081 ggatttgccg atgaaatcat cggtgagatt gacccggata ttccggttaa taattcgatg
    46141 tactcggaag tcaaagtaac taattcactg acttcagcga ttgctaagaa gtgtcgtatt
    46201 gaaccacctg aaccaaagga acgagaagaa attaataaca ctatcccagc cgattcgtta
    46261 atgagtcggc ttaatttaat gaaaaattgg aggaactaac ccatgaacaa aattcaagaa
    46321 ttgatggaaa aacgtgcaca agcttggaac ggagcaaagg cttttgtaga agcaaagcaa
    46381 gataaagatg gcttgatgtc tgaagaagat gccaagactt atgcggaaat ggaaacaaaa
    46441 gtgaagaact ttacgatgga aattgaacgt atgcagtcaa tggaagcgat ggatcgtgaa
    46501 cttgaaaagc caatgagcca accattaact tcgcaaccta tgactactga aaataaaatg
    46561 gaacctaaga aaacaggacg tgcttcagct gaatataaag aaggtgtact tgaagccttg
    46621 cgctcaaact tccgacatgt ttcaaatgtt cttcaagaag gtgtggatac ggcaggcggt
    46681 tatttggtgc cagaagaata tgactcacgt ttgattgacg gcatgacttc tgaaaacatc
    46741 atgcgtacac ttgcgacaac aattacgaca agtggtgaac acaagattaa tattgcaggt
    46801 tctaaaccag ccgcttcatg gattgaagaa ggtggcgagt tgagctttgg ggatgcaacg
    46861 ttcgatcaaa tcttgcttga tgctcacaaa cttcatgtgg caatcaaggt gactgatgaa
    46921 ttgctttatg acaacgcctt taaccttgaa aactatatca tcgaccaatt caccaaagcc
    46981 ttagcaaacg cggaagaaga tgcgttcttg aatggtgacg gtgttggtaa accactcggt
    47041 atttttgcag caacaggtgg cggacaagtt gccattacaa ctaatactca aagtgcagtt
    47101 acggcagatg atgtggtgaa ccttgtgtat gctttgaaac gtccatatcg taaaaacgcg
    47161 aagtttatca tgaacgacca aaccatcagc ttgcttcgta agttgaaaga caacaatggt
    47221 gcctacatgt ggcagccagc tttggtagca ggtgaacctg accgtctgct cggttatcca
    47281 gtttatactt cggagtttgt tccaaccgtt aaggcaggtg cgccagtcat tgcttttggt
    47341 gatttcagct actataacat tggcgaccgt ggcgtgcgtt cattcgacca gttgcgtgaa
    47401 ctctttgcag gtaatggcat ggttggtttc ttagctaaag aacgtgtgga cggtaaactt
    47461 gtgttacctg aagcagtgca aatcttgaag atgaaagcat ctgcctcatc ttaaggagta
    47521 gcctatgagt gacttactaa ataaggtcaa ggcaaacctc atacttgagc ataacgaaga
    47581 tgatgagctg ttggaacgat tgattctgac cgctctttct tatgctgaga gctatcaaca
    47641 tattgagtct ggctactatt cagaacatga aatgccgcca accactgaac aggcggttat
    47701 tgtgttagcc agtaactttt acgaaagccg tgatggttca actggtggct tctttgcgga
    47761 taacgtcaat gccagtaagc aagtctggga aacggtcaat ctgttactca ggctcgatag
    47821 gcggtggcaa gtatgagttt tggaaagatg aatcaacgaa tacaaatcgt taaagtgacg
    47881 aaccaaaagg atagcgctgg attttcagtg aaacaagatg aagttcttgc agctgtccat
    47941 gcatacaaag aggaaaagaa tgcgactgag aaatgggtaa acatggcgac ttttagttca
    48001 gcgacctgcc tgtttcggat tcgttatatt cctaatctag aggttacgac agagatggtt
    48061 gtcatttcag atcagaaaag gtatcagatt gtgtcggtgg agaatgtccg aaatcgcaat
    48121 atgtaccttg aaatgctgac tactgaaatg gaggtgagtg cggatggcta aagcattgat
    48181 gaagatgcca gaagatttct tgttaaaggt ttcaaaacta gaaaacaaga cagatgaaat
    48241 catccccaag gttttagaaa gtggtgctga agtggtggaa ggcaaagtcc gaagtaactt
    48301 aactgccgtt gttggctcaa atacaaaagt accaagtcgt tcaactggtg aacttgaatc
    48361 agcccttggt atcacacaag ctcgtcaaga taaaagcggc aactggaaca tcaaggttgg
    48421 ttttgatgag cctcgaatcg atggcagttc aaatgctaag attgcgaaca ttctcgaata
    48481 tggaaaacac ggtcagccag caaagccatt cttgaaacct gcaaaatcac aaagtcgcaa
    48541 gtcatgcatt gaaacaatga aatcaaaaat ggaaagtgag gtaaacggca tttgaacatt
    48601 ttagaggaat tgaacagtct ttttgccaca cttcatattc ctgttgaaac gggcgagttt
    48661 agtgataaag cgccagacaa gtattgtgtg ctgacaccac tcgctgacag gttcgatgtt
    48721 tacggggata actttccaac cgttgatgta caggaagttc gcatttcctt gtttgtgaaa
    48781 ggcaactatc tgaacacgca aaaacaactg gttcagaact tgttgaaaca cgagtttacg
    48841 attactgaac gattatttgt aggatttgaa aaagatacaa attactatca tgtggcgatt
    48901 gatgtcgcca agcattatga aatggaggtc tgaaaatggc aacgattgga ttggataaat
    48961 tatattatgc accgattaca gagtcggcaa ctactggcga agaaacatac ggcacacccg
    49021 ctatgcttgc gaaagcaatc tcagctgagc tttcggtgga acttgctgaa gcaactttat
    49081 acgccgatga tggtgcatca gaagtagtga aagaatttaa aaatggaaag ttaacgctcg
    49141 gtgttgatga tattggacgt gctgtggcgg cagtgcttgt tggtgcaacc gtggatcaaa
    49201 acggtgtctt aatttcgaca accgaagatg gtggcacacc agttgcgatt ggtttccgtg
    49261 ctcgtaaatc gaatggcaaa tacaaatatt tgtggttgta ccgagtgaag tttgccgtgc
    49321 ccagcacaag cctagcaaca aaaggtgacg acattacatt ctcaacacca acaattgagg
    49381 gtacagtcat gcgtcgtaac aagttagatg ctagtggtaa gcatccatgg aaagcagaag
    49441 ttgatgaaaa cgatacggga gttgaacaaa caactatcaa tggatggtac acgagtgttt
    49501 atgaaccaga tttcacagca gactaggagg caacaatgag tgaagaacgc agtgccaaga
    49561 ttagccttgg cggagatgaa tatgaactga ttttgacaac aaaagccacg aaagaaatcg
    49621 ccagtcacta cggtgggctt gataaccttg gtgagaaatt gttgaaatcc gagaactttg
    49681 aattggcgct tgatgaaatc atctggttga ttacgctgtt ggcgaatcag tccatcaaga
    49741 tttacaactt gaagaacaca gaaaagaaag atgaactcac gactgaatat gtggagttga
    49801 tgacgagtcc gttgcagttg gctgaatata aatcagcaat cactgaagca atgttcaagg
    49861 gcacagctcg caatatcgaa agtgaaacag attcaaaaaa caaagcaggc gagtaagtga
    49921 tgaagaaaca tttactcgcc tgtattacta tgggacagtt caaatgggta tgacgtcaga
    49981 tgatttctgg tactgctcgc ttggactgtt tttggattta tgggagtgtc ataagcaatt
    50041 tactgggatt agcaagccga aagttgagtt attcattgat gatgtgatac cgagtggaat
    50101 ttaagggaag gtggtgattt atttggcaga taattttggt ttaaagattg gcgttgaagg
    50161 tgaaaaggag tttaagaact cgttgcgtga aatcaaccaa tcattcaagg tgctaggttc
    50221 agaaatgaaa ctggtatcat ccgaatttga taaaaacgat aaaagcatca gctcactgtc
    50281 tgctcgcaac caagtcttaa acaaatcgat tgatgcccag aaagaaaaaa tcaccaccct
    50341 tgaaaatgct ttgaagaacg ctagtgacag ttttggtgaa aacgataagc gtactcagaa
    50401 ttggacagtg caattaaaca atgcaaaagc ccaactcaat aatatggagc gtgaagttgc
    50461 taacaacgag aaggcaattg acgccatggg tactgaagaa gttgatgctg ctaagaaaac
    50521 tgatgagctt ggcaacgaac tcaaggatac aggtgacgag gctgaaaagt ctggaagtaa
    50581 atttgaaaag ttcggtggcg ttctaaaagg tatcggggta gcaatgggta cagtggcact
    50641 tgctgctggc gctgctgccg tggagctagg aaaacaagtg gtatctgctt acgcggacta
    50701 tgaacaatta gtcggtgggg tggatactct tttcaaggat gcgtcatcca ccgttcaaaa
    50761 gaatgccgac aatgctttca aaactgccgg aatgtcagcc aatgaataca tggaaactgt
    50821 cacaggtttc tcagctgcct tgatttcatc tctcggtggt gataccaaga aagccgcagg
    50881 agttgcggat caagctgtta ctgatatgag cgataatgca aacaagatgg ggacggatat
    50941 tgagtccatc cagaacgcct atcaaggttt cgccaagcaa aataacggga tgctggacaa
    51001 cttgaaatta ggttttgggg gcacgaagtc cgaaatggaa cgtttgctcc aagaggctga
    51061 gaagatttct ggtgtcaaat atgatatctc gaacttcgct gatgtgactg aagcgattca
    51121 cgttattcaa accgaaatgg gcatcactgg tacaaccgcc aaggaagcga gtgagacgat
    51181 aagtggttcg attgacggta tgaagtctgc aatctcaaac ttgatggctg gacttggtaa
    51241 cagcagcgct gacattggta tgttgattgg caatgtggtg gaacaatttc aaaacgtcat
    51301 caagaatatt ctaccggtca ttcaaaatat cgtgtcggct ttaccggcag tggtggatgc
    51361 cattattcaa gcattggggc agttattgcc aacactctta gaagcagtaa cctcattatt
    51421 ctcacaagtg cttgaaacga tacttggctt acttcccaca cttattccag tggtggttca
    51481 agccgtttta acgattgttc aaacacttat tgagaactta cctttactga ttgacgctgc
    51541 ctttcaactt gtgacaacac ttatccaagg actaggcgaa gcgctgccag aacttattcc
    51601 aattacagta caggcaatca taacgattgt gaacggcttg attgaaaact tgccattact
    51661 gttggatgct gctttacaac ttatcatggg acttgcccaa ggacttatca cagcacttcc
    51721 aatactgatt gaaagtctgc cacagattat tacaggaatc gtgaacttta ttatcagtgc
    51781 tattccacaa atcattcaga ctggtattca acttttgaca tcacttgtga cagctttgcc
    51841 ggatatcatc aatgcgattg ttgcagctat tccagtgatt attcaaaata tcatcaatgc
    51901 cgtgattggg gcaatccctc agttgattga cgcaggtatt cagttacttg tggcattgat
    51961 tggtgcgtta ccgcaaatta tcacaacgat tgcgaatgcc atgccgcaaa ttatcaacgg
    52021 aataacagga actttggtaa acaacatcga caaaatcatc aatgctggtg tgaagttgct
    52081 tgtggcatta gttcaaaact taccagcaat catttctgcc attgtcaaag cagtaccgca
    52141 aatcatcaca gggcttgcga atgctttcgg tgagtatgtt tcaactatgg gaactatcgg
    52201 gaagaacttg attacaggat tgtggaatgg tatcgctgat gctggaagtt ggttacgcaa
    52261 caagatttca ggattctttg gcggtgtagt agacaacatc aaggacttct ttggtattca
    52321 ttcaccatca acactgtttc gtgatgaaat cgggaagtac atggcacaag ggattggcgt
    52381 aggttttgat aaggaaatga ctggtgtttc taaaacgatg cagaaagcta ttccaacaaa
    52441 actttatatg ccaagctttg atattgatac cgatatccac gctgccgttg atagcacatt
    52501 ctcgatgctt gatttaggtg ctaaggttga tgccttaaca aacatcacga gtgaaatgtt
    52561 gccaacgcta cttaaagcaa tggacattaa agtcgtcttg aatgatggaa cattggtcgg
    52621 caaacttgct ccagaaattg ataagagctt ggcgacgttg aatcgtagac gtgtagcata
    52681 ttagtgttgg attttcacat tttgactgta tactataact aacgagtgtt tactgaagag
    52741 atggtggtaa tcatatgttg aagcataaat ttaaagcaaa gaaacctgca ctgataggat
    52801 atattctact gcttgctatt ggattatttt taacggcagt aagatggtat agcggcataa
    52861 atcatggttt tgtattcatc aacaatcaga ttaattctca catttcaaac ttcgcattaa
    52921 gctttctttt ctatttggtg ataggctatc tttggttgct tatgggaacg aatttaaggg
    52981 ttcttactgc gatgggagct ggtctgattg ttgcaaatat tgtatgtgaa acgatgatga
    53041 cttttctgaa tacaccagat ctaatcgatg caatatatgg aatttccggc gtgctggttg
    53101 tatacgcgta tttagcgatt accaagaaaa tagggctgga agaaacaacc gaataggatg
    53161 atagattata aactttagga ttagacattg ttgtctggtc cttttttagt tatttagatg
    53221 ggcggtggtc aattgaacgc atttatttta gacggaacaa ttaattcacg gactgattta
    53281 ggtttgcgaa ttacggaagt gccagttatt ccagtgacgg aacgtgtcat tgatagtatt
    53341 gagattgatg gacgtgaagg agatttgacc attctgaaag gctggaaaga tgtgactttc
    53401 ggttttaagg tagtgatttg gaaatctgat gtttggacta gctggcagat aattttgcca
    53461 caaatcctaa acgctaaaac tatcgcattt tcaaatgaac caaacgtgtt ttacccaatc
    53521 aagtatgcta aggcgagcgg actaactcag attttatctt ccatgtggga gtttgaaatg
    53581 gagttaacct gttcgccttt tcgttataag accaatgtag cgactatcaa tcgaaccacg
    53641 tcaggaacgg tcaataatcc cggcaacatt tactcattgc cgaagattaa ggtctacggc
    53701 aatggaacta ggacactcac gataaatggc aagcctatcg tgttaaatct gacgaatggc
    53761 aacctgacac tagattcgga tttgaaagaa tgcttttacg gggatgtggc agccaatcaa
    53821 tacatgacag gtgactttcc tgagtttcga gtcggcagta atactgtgac tcttggtaca
    53881 ggtataacca aagtagaaat agaaccgagg tggcgatact tatgattacg ctttacaaca
    53941 aaacagaatc aaatttcaat cataacggtc tcgctgtcct tgatggcaac attctgaacc
    54001 ccgtggtgag tgaggaattg aacggactct tttcctttga attcgattat ccaattcatg
    54061 ctccacacag tgaggaattg ttgccagaaa tgattgtgaa agcaccagta ccagaactgt
    54121 ccgaccagct tttcaggatt gttgaacgag atgatgcact tggtggtttt ttgcacatag
    54181 tagctcatca tattttctat gacctagcca caaactttat tgaagatact tatatcgtca
    54241 acaagaacgg gtcgggagct ttgacacaac tgcttggtgc aactcaattc atgcaccgtt
    54301 ttaccggtac atcaaacatc agtgcggtca ataatgtccg cttggtgcga ctaaatcctg
    54361 tagaggtgtt gcttgatagt gacctagaga atggttttca aagcagttat ggtggtgaaa
    54421 ttgtacgcga taacttcacg atttcaatgt taacgactcg tggcagtaac aacggcgtac
    54481 aaatccgaga caagaagaat ctgacaggat ataaagctga tgttgattac tctggaattg
    54541 ttactcgcat tatgccacaa gggtatgatg gactatttct tcccgaaaaa tacgtggaca
    54601 gtccaaacat caaccaatac gtccagccta aaatcaaagt catcaagtac gacaacgtca
    54661 aagttggtga tgaagaggat gagtttactc aagccgaggc ctatgatgaa cttcgcagat
    54721 tggctgggtt agaatactca gctaacaagg tcgatttgcc aaaagccacc tatgatgtgg
    54781 agtttgcccc gcttgagcga actgaagaat ataaagattt tgcacagctt gaaacgatta
    54841 atttgggtga tactgtttca gttattcatg ccgaagatgg attgaacatc acagctagaa
    54901 tggtctcata caaatacgat ccatttctga aagcgtatat ctcagtaaca ctcggaaaca
    54961 tcacgccaaa attcacggat gtggcgaagg acatcaaccg ggtggacaac aaggcggagc
    55021 aagccaaaga cgatgccaat tacgcattga gttcggcaaa tggtaaaaac accaacttct
    55081 atggcccgac aactccaaat aatccgaaac tcggtgacgt ttggtacaaa gaaaatggtg
    55141 acaagtttga aatgtgggtg tatgaaatcc gtgatggcgt cactcagtgg tatccactta
    55201 tgacagatat gacggcagaa gaagtaaagc aagccgtgga acaagcacaa aaggatgcca
    55261 atactgccac tgaaaaggcc aatcaagcat ttaacgatgc tgtgaatgct ttgaatgcag
    55321 ctgaaagtgt aggtgcaagt gttgaaacgc ttactacatc cgttgaacag aatactagca
    55381 agatttcatt agtggcgagt ggtatgtcaa ctcttgaagg acgagtaatc acagctgaat
    55441 caactctgac agtgcaagct gggcagattg ctagtaaggc aagtcaatcg acggttgata
    55501 atctgacagg gcgtattaca actgcagaaa gcaccatcac ccaaaatgct gatagatttg
    55561 agctatctct ttctaaaaca aataaagcgg tcaatgtttt agccggtatg agcattgcag
    55621 atttgcagtg ggcagttggt ggtctgaata caaatgatgg aaaagaaaat acctatggtg
    55681 gatacgttcg ctcaggtttt acaagagtta gaaatggcga aaagtatcta cttcaaacat
    55741 ttgtgggtgg gaaactttac tctacttacg caacggctta tctgctttat tacaaagagg
    55801 ataagtcctt tttatcgtac acccaatttg gaaacaccac cacaccgctt actgttccaa
    55861 gcagtgccgc ttttattcgc attcgtttaa caactagtga tgcaccagaa accatcagtt
    55921 gctatatgtt gaaaacggat gtgacaaatg gctatgttga tttatccgag ataagtaacg
    55981 tcgtaaaact tacagcgacc actgatgcct tactgttaag tgtttcagaa accaaagaag
    56041 caatcggcgt tccatttaaa gtcaatcaat gggaacaagg aagtttgagt accactgacg
    56101 gttcagaagt gtccgcaacc aattatctcc gaagtgagtt ccttgatgtc aaagtgggag
    56161 ataagtatat cgggcaactg cgtgacggaa ctcaagctgc tttctactat cattactacg
    56221 gcatggattt accttacatt gattttgtcc caacggcaaa acaatttagt gaaaccctaa
    56281 cggcgggaac ttatactaat gcgaccattg cgagttatct agtaagtaag aacgttgaat
    56341 cgatgagtat tacaagtggc gtgacaacaa acccctattc aaattcagga gattatctgg
    56401 tcatttacaa attgccattg gaaggcaaag tcgctccgag tgtaatttcg tggaatggtc
    56461 gcaaggatac atcagataat attgtcttgc tttatacagg tggtgcttgg gaacagattt
    56521 cgaccgttac aagtagcagc aatgccactt attcttggac gttaacggct gagcagatat
    56581 cagggattac tactgacagc gtgtatctag ctttcttttc agttaagact ggcacatatg
    56641 ctgggattta taattcaagc aacacacctt ttactctaaa cccgtcggat ggttcaagtt
    56701 atcatcaaat ctcttatact ggttcgagcg gagcaattac agtcccgagt ggcacattga
    56761 aaatgcgagt ccgagtgaac acgagcatca aacccgatga atatgaagga aatatttatc
    56821 ctacgaccac aaggcaagac tataccaatg ccaacacatt atattcagca attctcatgc
    56881 agaaagactt aatcaattta cgagttggta aaggagacgt gattaatcaa atcaatatct
    56941 cacctgaaag tattttgatt gctggaaaca aggtgcacat cactggtcaa acaaccattg
    57001 atagtgcagt gattagttct gccatgatcc aaaatgcggc catcacaaac gcaaagattg
    57061 cagatgccac aattcaaagt gcaaaaattg caagcattga tgcggcgaag attaccacag
    57121 gaactttgtc ggcagcccgt attggcgcga actcgattac ggctgataag attgctgcta
    57181 atatcctaac agcattgact gcgagttcgt ccattcgaat cacaggaact acgattggat
    57241 attattcagg aagcactctt gtgactgaaa taaactcgca gggaatgacc attcgacgtg
    57301 gtggaacgac agtcggaaca attggtgcaa ataacatcag tggtcactct gattggcgtg
    57361 gattggtatt tgatcttgag tatggaacag agtatatgtc atgggctcat aaagattcct
    57421 cctctgcgag tgtctacacc cagaagcttt cttggtatgc gagaacattg cagtctggta
    57481 tggagaaggg gttccacttt gacgacgaca tttatttacc atcaatgctt cgtataggga
    57541 cacaacatca aatatctgtt ggtgggatta catttagtag ttccctttat actaagattg
    57601 gctcaagttc cggcagcgct ggtattgcat tcaacggttc taatctgatg ctcggagatg
    57661 atgggaagtg ggttgacttt ggagtcatcc gtgaaatctg tgccaaactt gcgggacgaa
    57721 caatcgcttt accaaccggt ttcagtagca acggtacagc gagtggttgg tacaatccgc
    57781 agtcgtttaa ttcaatgacc acatacagtt aggaggccgc catgaacgaa gaaaaagaag
    57841 aagtgatagt aactgaacct atcgaggcag atattccaaa gccaatgaaa gttgggacgg
    57901 atatggttga tttgcttgtg atggtcgtgg atgatctttg tgtccagatt ggtgatttaa
    57961 aggagaagat agataatgct caaaattaga aatgaagatt tggtggacgt gatgagtttc
    58021 ttaaaaggtt ttgacttacc accaaagatt agccgagtgc gaacgaagct tgcaaaactc
    58081 atccaggtga aggttgatga actctatcaa gacgaggtag acttgcttca aaaatacgga
    58141 aagaaagatg agcacggcaa tttggtccaa catgacggtg ccttttcact catcccagaa
    58201 accgcaactg aatatcacaa agaaaaggca gacttgctca acgaagaaac ccacatcaac
    58261 gtggatgaac ttcaagacaa gctgccttta ttggttgctg gatttgaaaa tagtgaagtc
    58321 accatttcag ggaaagatgc tgaaatgctc gatttaattt tagaacaatt ggaggatttg
    58381 taaatgaaag aagtgtggaa ttggattcaa ctcgccatcg caggtggtgg aggagtgatt
    58441 ggatggtatg tgggaggaat tgatggtttg atttatacgc ttgtaacttt tgtggtagtg
    58501 gactatgtaa ctggtctgct tcgtgcaatc aacgaaaaga aactatccag caagattggg
    58561 gcaaagggca tcactaagaa aatcatgatt tttcttttgg ttggtgtggc tcatatgctt
    58621 gatgtggaag tcatcaaagg tggaaatgta ctgcgtgatg cggtcatttt cttctatatc
    58681 tcaaacgaag gaatttcact gctggagaat gccgtggcga ttggactgcc agtgccggaa
    58741 aagttgcgtg aaatcttgca gcagttacac gaaaagaagg aggaaacaga aaatgggaat
    58801 tagtaatcta gcaacaaaat atggatttca gtcatttagc cattactcga atggtcgtag
    58861 tggtgccaaa gtagataaga tagtgattca tcacatggca accaccaact atgatattgt
    58921 tcctgatatt tggaaaacgc gtgaggcgtc agcacactac ggtattggaa aaaatggaga
    58981 aattcgagca tacgtggacg aaggaaacac agcttggcac gctggtaatt ggaacgccaa
    59041 tatctcaagt atcgggattg agcactgcaa tagcactgga tcaccgaact ggggtatcaa
    59101 tcaagcaact attgatgcaa gtgcaaagtt gtgtgcagat attgccaaac gttatggtct
    59161 agggaaattg gtggtgaaca agaatatctt ccctcactca catttttcgg ccacatcatg
    59221 tccgggacag ttgcttggta aactgcagga gattgcggat aaagccaatg cgattaataa
    59281 tggttcatca accccaagca cgccagctag tggtctgtat cgtgttcgca aatcatgggc
    59341 tgacgccaag tcacaaattg gtgcctataa ggacttgaac aatgcgaagc gggctgcaga
    59401 tagccattct ggttataagg tgttcgacca gaacggtaat caagtttatc catctgggtc
    59461 atcaacatcc aacgtgaaaa ctcttgtggt tcaagtgagt ggtctcaacg ttcgcaacaa
    59521 gccaagttta agtggtgcgg ttgttggttc atacaataaa ggcgcaactt ggacgcttcc
    59581 gaagtcagag aagatgacga ttgcggatgg ttgggtatgg gctcgttgcc cgattggata
    59641 ttgtgctgtc ggtaagaata ctggtaagac ggaatctgac gattatattt tgatttcata
    59701 aatttaatcg gaacaaaatt atgtccggat tgttgactta aaatatcgaa cgtgttatta
    59761 ttaattcaac cagaacataa atttgttccg attgaattat agaaagaggt atcgatatgg
    59821 ttacgtttaa agatttgtta ccagaaccaa cagaaagcca acaagctttc attttagatc
    59881 acgggaaaac agaagatgat ggtcagttga aatacgctga tgatgctaaa agttacggtt
    59941 ggaacatgcg ccaatatggt aaattgaaag ccggcgcggt tgttttgaat cgtcatccgg
    60001 ggaaaattac taaagaccgt aagtgggaaa tttacggggg cggatacgtt gagagcattt
    60061 ctgaacctga tgaagctgga aatgttacgg cagtaattac acacgcattt acaatcgaac
    60121 cggcaattaa acaaggtgat tcatttattg aaaacttcga ttggaataca cctagcaaaa
    60181 agaagcgtaa aaagccgaat tcatgggcct acttctggga tcagtatgga atgaatgaaa
    60241 ttacctatgc cgattttgtt gcattgattg aaaatcgtca tcttatccca atcgatgata
    60301 cacaatcgtt gccgtctgaa ctggatctta caaacgagga agttgaagaa attgagcaag
    60361 aaagttcaaa aggatttact gttcttgttg atgaagtggg accaaatcgc acgaatggga
    60421 ctcagaaacg aaaattcact ggacgccata ctgattggga gcgtgtcaac aaggcaaaac
    60481 aaaaaacggg tgctctaggc gaagaaatcg tacttgattt cctcattcag gaagcagaaa
    60541 agaacaaaac aaaacttcct gaacatgttt ctaaaactga aggagatggt catggctatg
    60601 atatttgtgc gtttgaccaa tcaggtaatg aaatccatat tgaagtgaaa gcttcaaaaa
    60661 caaacttctc tgacggattt gaaatgagta ctaatgaggt tgctgcctcc cttggagata
    60721 caccatataa aatttacttt gttcacgacc ttgatgtggc gtcaaaagtt tgcaaaatca
    60781 aaatttatga tggcccaatt actgaagaaa acttcacgat ggttccaacg agctacaaaa
    60841 ttttcaataa gtagtttgac gtctttataa ttcaaaacag cctcacaagt tttattgtgg
    60901 ggcctttttt tcgtctagtt ttctttaagg taggtgttta cagcattttg cattaggatt
    60961 aaatgatttc ttaactgaaa tacctgctct ttgtaattga tgcagaaaag aggcgattct
    61021 attgattcca tacgattatt catcgcgttt atattggctt gtaaaaactg gtataaagct
    61081 tcttcagcct ctatggtttt aaagcacttt tcaaattctt gtttgaagct ttcatcaaat
    61141 tcctccagtt cctttgttgt cgcagatttg caaaaatgtt catattcttt ttctaaccct
    61201 tccatatcaa aactactcat aactttctcc ttcccacaat tgatatttat attgttattt
    61261 attggatgaa gcttgtaaag gcttatttga tgtttgggaa gtaaaaagtc aaaatttaca
    61321 aagaataatt cctactatat ataaacaaac ttagaagcct gcaagagtga ttcgtttcat
    61381 tcttgcaggc ttttttcgtt ttgtgggttc gagtcggtta tttctttcgc atatggatga
    61441 aaggcaaagg aaaatttgtg attgggttta ctttcatgga ttttcttcgc ctgtatcttg
    61501 aggaggcgaa agcaatgact aacaagaaaa cagaaattaa ctattcagat gaagcagcct
    61561 attacatttc acagcagatc cttctgaact tacttgttac cggtaaaatc aacctggaag
    61621 aatacgtcaa aatcgacaat aaaaaccggg aaacattcaa tcctttttta tcaaggataa
    61681 tggtctgaaa cgttgatata acggcacttt agagctaaca tgtcacctaa tggaaaggag
    61741 ttgagctgat gaaaacgata cataaattgg cacgtccaga gttgccggaa gcttccaaac
    61801 taaaggtggc agcatacgct cgtgtatcaa catcaagtaa tgaacagctg gcaagtttac
    61861 aaacccagat tactcactat gaaaatcaca tcaagaacca tgaacaatgg gactatgtcg
    61921 gtgtttatta tgatgaaggg atttctggaa ccaagactgt aaaacgtgat ggtttaaaac
    61981 gattgattaa ggatgcagag cttggaaaga ttgatttgat tctgacaaag tctatttcca
    62041 gattttcaag aaatacagtt gactgcttga atctggttcg gcagctaact gatattggtg
    62101 tcaccatctt ttttgaaaag gaaaacatta atactggtga tatggaaagc gagttgttgc
    62161 tttcaatttt atcaagcttg gcagaaagtg aatcctattc ccattcagaa aacttgaagt
    62221 gggcgattcg aaaccggatg tctaaaggga aattcaaagc ttacccgccg tatggctaca
    62281 agcggaaagg ctatgatttt cacatcgttg cagctgaagc aaaagttgtt aaacaaatgt
    62341 tccagtgggc attagatggc atttcgactg gaatgattgc caagcggtta aacgatggaa
    62401 aacaattcac taaacgtggg gcaaattggc aagcaacatc agtcagaagt attttgaaaa
    62461 atgaagttta taccgggact ttgacgatgc aacaaacctt tgttgatgac cagtacaaac
    62521 gacgcataaa caagggcgaa ttgccaatgt accagatcaa aaacaatcac gaacccatag
    62581 tatctaaaga agattacgac cgagttcaag aactcattcg gagaaaagct gaggccaaag
    62641 ggaatactgg tgacgggaaa aagtacacca agcgatacgc attttcaggc aacatcatct
    62701 gtgataaatg cggcagcaaa ttcaagcggg ttcacactta ttggaaaggt aatacgaaga
    62761 aggttcagtg gaagtgtacg gggcatctta aaaataaaga tagctgcgat gcattaccaa
    62821 ttacggatga agatttgaag gttgcttatt tgaccatgtt gaataagttg attttcggac
    62881 acaagttggt tctggaacct tttatgaagt ctttgaataa agcaggtgcg aatcaggaag
    62941 ggctcgaagt gcttgctgat gaaatcgaca agattcaaga aaagcttgag gttctggcaa
    63001 gtttgactgc ttccagtatt ttatcaacga aggtgtcttt agaagaacaa agcaaacttc
    63061 aacaagagag ggctgtcgca caagaaaagc agcgcctcat cattgagtca gtcaacggca
    63121 aaagcactca gcaaattgaa ctagaagcct tatacaaatt cgcgcggatg gcagagatgt
    63181 tgacggactg ggatgaagat ttattcaaga agttcacaaa cagcattaca gtttacaacc
    63241 gccaagaagt cggatttgaa cttaagtgcg gactactttt gaaagagagg ttaccagaat
    63301 gaacaataca attccatacg ggtatgtcat taagaatgga gaaatcaacg tggatgaaca
    63361 gcaagctcaa caaatacgtg agttcttcaa tatttacctt gagggtgttg gcttacaaga
    63421 agctggcaaa cgctctggca ttaacaagcc gcatcccttt atgaaacgta ttttgatgaa
    63481 tgaggtttac ttaggaaatg agatatatcc aaaaatcatt gatgaggata ttttcttcca
    63541 agcggatctg gagcaagaac gaagaattaa gttctttaaa aaacgcaagg agatcaaagt
    63601 gccagatacg aaggtaatac ccactcattt taaatctggc aagattaccg aaaagtattc
    63661 tgacccattc aaacaagcgc aatatgcata ttcaaaaatc aaggaggtta agtgaaatgg
    63721 caggaaaaca ggtgacggtt attccaatga agcctagaca atgggcagca gagaataccg
    63781 aagagaaacc aaagttgaaa gtggcagcct attgtcgtgt ttctactgaa atggaggaac
    63841 aggcgagttc ttacgaagca caggttcaac actatacgga ctacattcaa agaaatcccg
    63901 attgggagct tgtaggaata tttgcggatg aagggatttc aggaactggg acgaaaaagc
    63961 gtgacggttt taaccggatg attgaggcgt gtcaaaacgg cgaagttgag tacatcatca
    64021 caaaatcaat ttcacgcttt gcccgtaata cggttgattg cttgcaatac atacgacagt
    64081 taaaagaact gaagattgca gtgttttttg aaaaggaaaa catcaataca atggatgcga
    64141 agggtgaggt acttttgacc attatggcaa gtctggccca gcaagaaagc cagtcactaa
    64201 gtcaaaacac caagatgggc gtacagtatc gtttccagca gggacagcta cgtatcaacc
    64261 acaatcattt tcttgggtac actaaggacg aagatggtaa cttggtgatt gaaccaaagg
    64321 aagcagaagt catcaaacgc atcttccgcg aataccttga gggcagttcc ttgcaagaaa
    64381 ttgctaatgg tctaatggct gatggcatcc taacgggagg gaagcgaaag ctctggcgag
    64441 gagaaggagt cagattgatt cttcgaaacg agaagtacat gggggatgcg ctgcttcaaa
    64501 aaacatatac taccgatttc ttgacaaaga aacgagtgaa gaatgacggg agttacgctc
    64561 agcagtatta tgttgaagat agccatccgg ccattatccc aaaagatatc ttcatgcaag
    64621 cccagcaaga actagaccgg cgtaaatcaa tgaaaaacaa aaagtcacag tgcttttctg
    64681 ggaagtatgc gttatctggt atcacagttt gcggtgactg tggcaatgct taccgcagag
    64741 ttcattggaa gaatcgagga actgtgtggc gatgcaaatc cagagtggat aaacgagaac
    64801 ataattgcac gggtcgaaca atctacgaaa cagacctgca tggagctgtt atcaaggcaa
    64861 tcaatgaaac gttaatcgat cgtgatgctt ttcttcagca attaaatgac aatatcaact
    64921 cagttatgac tgatggatta actgaccgat tggcagaact agacagtcag ttgaaagagc
    64981 ttgaagctga aattattagc ctggcagttg gcggtcaggg ttacgatgaa ttggcaagcc
    65041 aaatattttc actaagagat gaacgagatg cggttgcaaa acaaatagca gcgaatacta
    65101 atttgcaaca acgggttgat gagatggtag tctttgtaaa agaacacgat gtcatcaatg
    65161 agtataatga agtgcttgtt cgaaggttga ttgagaaggt caccattttt gaaaagaaca
    65221 ttgttgttga tttcaaaagt ggggtgagag tgactgtaga aatttaattt gaatagatag
    65281 gactagacgt taatgtgggt agccatatta acgtctttat tgattggatt tcataaagtg
    65341 attgatgata taatatatta tgtaaaaaat aggctggggg cgaagttctg tgagccaatg
    65401 gaataccatt gatgaattaa gggatatttt tattcgtaaa tatacggatt ctgagttgag
    65461 taatgaactt gaaaaggcct gcagcgaaga atatgaattg atgcgtgatt ataatgggcg
    65521 tcagatttta gagttattac agaatgtaga cgatgcctat ggagatataa aatcagaaaa
    65581 tgatgctagc aatgacgaag tgaaagttca gataacatat aaaaataata tcttggaagt
    65641 tgggaataca ggcacttcat tttcaaaaga tacaattgaa agattgtgct taggaagagc
    65701 gagtaataaa tcatcacaaa atatcggaaa taaggggact ggttttcgtt ccttgctaaa
    65761 cgatgctgag tgggtcgaac tgtattcggg agaatttgct attcgattta gtgaagaatt
    65821 cacaaaaaag ctttttgagc agtatgtatc aagagattct gacaaattca gtgaattaat
    65881 ttttgagcag aagaaaaatt ggaaaaaaga agattatgat ttatgttttc caataatgaa
    65941 ctgtcctcaa ccaataaaca aaattgattc tggttttgat acattaattc gagtaaaact
    66001 aaaagaaact aatttaagta aaggcacgag tgtttctaaa caacttcagc agccatttta
    66061 taagtcactg ttatttttac caaatataac aaaaattgtt attgaaacaa ctgatgatga
    66121 aaaaatatca gagaaaattg tggatggaaa tgatgtattg atagaaaaaa gtgatgttac
    66181 tggtcaatca ccgtctgaag aatattttgt ttttgacaaa gaatctatga taggagataa
    66241 agttgctaaa attgcactag ccgtttctaa agattctgat tacgattact ccaaagaaaa
    66301 actatattgc tactttccag tacgtaattt tggtacacca attcatgcac ttataaatgc
    66361 accgtttata acaaataatt cacgtgatga cgtcccggat gata
//
LOCUS       DAZBTF010000006        64886 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000006 DAZBTF010000000
VERSION     DAZBTF010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 64886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.006"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1420)
                     /locus_tag="ACHGNS_000443"
     CDS             complement(<1..1420)
                     /locus_tag="ACHGNS_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HFN6936424.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAG"
     gene            1672..2853
                     /locus_tag="ACHGNS_000444"
     CDS             1672..2853
                     /locus_tag="ACHGNS_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFN6936425.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(2913..4541)
                     /gene="treC"
                     /locus_tag="ACHGNS_000445"
     CDS             complement(2913..4541)
                     /gene="treC"
                     /locus_tag="ACHGNS_000445"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFN6936426.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(4992..7703)
                     /locus_tag="ACHGNS_000446"
     CDS             complement(4992..7703)
                     /locus_tag="ACHGNS_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFN6936427.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7713..8414)
                     /locus_tag="ACHGNS_000447"
     CDS             complement(7713..8414)
                     /locus_tag="ACHGNS_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6936428.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8600..9199
                     /locus_tag="ACHGNS_000448"
     CDS             8600..9199
                     /locus_tag="ACHGNS_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFN6936429.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9280..10293
                     /locus_tag="ACHGNS_000449"
     CDS             9280..10293
                     /locus_tag="ACHGNS_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFN6936430.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10401..11318
                     /gene="rbsK"
                     /locus_tag="ACHGNS_000450"
     CDS             10401..11318
                     /gene="rbsK"
                     /locus_tag="ACHGNS_000450"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFN6936431.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11346..11741
                     /gene="rbsD"
                     /locus_tag="ACHGNS_000451"
     CDS             11346..11741
                     /gene="rbsD"
                     /locus_tag="ACHGNS_000451"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFN6936432.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11741..13114
                     /locus_tag="ACHGNS_000452"
     CDS             11741..13114
                     /locus_tag="ACHGNS_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN6936433.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            13471..15615
                     /locus_tag="ACHGNS_000453"
     CDS             13471..15615
                     /locus_tag="ACHGNS_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFN6936434.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLRLDKSFWLDLPLKK"
     gene            complement(15667..16707)
                     /locus_tag="ACHGNS_000454"
     CDS             complement(15667..16707)
                     /locus_tag="ACHGNS_000454"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFN6936435.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(16984..18786)
                     /locus_tag="ACHGNS_000455"
     CDS             complement(16984..18786)
                     /locus_tag="ACHGNS_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6936436.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(18800..19552)
                     /locus_tag="ACHGNS_000456"
     CDS             complement(18800..19552)
                     /locus_tag="ACHGNS_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6936437.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(19561..19923)
                     /locus_tag="ACHGNS_000457"
     CDS             complement(19561..19923)
                     /locus_tag="ACHGNS_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFN6936438.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(20099..20971)
                     /locus_tag="ACHGNS_000458"
     CDS             complement(20099..20971)
                     /locus_tag="ACHGNS_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFN6936439.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(21022..21510)
                     /locus_tag="ACHGNS_000459"
     CDS             complement(21022..21510)
                     /locus_tag="ACHGNS_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFN6936440.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(21648..22439)
                     /locus_tag="ACHGNS_000460"
     CDS             complement(21648..22439)
                     /locus_tag="ACHGNS_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFN6936441.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(22494..23102)
                     /locus_tag="ACHGNS_000461"
     CDS             complement(22494..23102)
                     /locus_tag="ACHGNS_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFN6936442.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(23095..23412)
                     /locus_tag="ACHGNS_000462"
     CDS             complement(23095..23412)
                     /locus_tag="ACHGNS_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFN6936443.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(23568..24296)
                     /locus_tag="ACHGNS_000463"
     CDS             complement(23568..24296)
                     /locus_tag="ACHGNS_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFN6936444.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            24455..25336
                     /locus_tag="ACHGNS_000464"
     CDS             24455..25336
                     /locus_tag="ACHGNS_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN6936445.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(25573..26616)
                     /locus_tag="ACHGNS_000465"
     CDS             complement(25573..26616)
                     /locus_tag="ACHGNS_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN6936446.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(26619..27287)
                     /locus_tag="ACHGNS_000466"
     CDS             complement(26619..27287)
                     /locus_tag="ACHGNS_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN6936447.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            27429..29765
                     /locus_tag="ACHGNS_000467"
     CDS             27429..29765
                     /locus_tag="ACHGNS_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFN6936448.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(29880..30191)
                     /locus_tag="ACHGNS_000468"
     CDS             complement(29880..30191)
                     /locus_tag="ACHGNS_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936449.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            30372..31847
                     /locus_tag="ACHGNS_000469"
     CDS             30372..31847
                     /locus_tag="ACHGNS_000469"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFN6936450.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            31894..32478
                     /locus_tag="ACHGNS_000470"
     CDS             31894..32478
                     /locus_tag="ACHGNS_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFN6936451.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            32480..32863
                     /locus_tag="ACHGNS_000471"
     CDS             32480..32863
                     /locus_tag="ACHGNS_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFN6936452.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(33180..33464)
                     /locus_tag="ACHGNS_000472"
     CDS             complement(33180..33464)
                     /locus_tag="ACHGNS_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFN6936453.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(33816..34526)
                     /locus_tag="ACHGNS_000473"
     CDS             complement(33816..34526)
                     /locus_tag="ACHGNS_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFN6936454.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(34523..35995)
                     /locus_tag="ACHGNS_000474"
     CDS             complement(34523..35995)
                     /locus_tag="ACHGNS_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFN6936455.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            36133..36606
                     /locus_tag="ACHGNS_000475"
     CDS             36133..36606
                     /locus_tag="ACHGNS_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFN6936456.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(36759..37310)
                     /locus_tag="ACHGNS_000476"
     CDS             complement(36759..37310)
                     /locus_tag="ACHGNS_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFN6936457.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            37415..37945
                     /locus_tag="ACHGNS_000477"
     CDS             37415..37945
                     /locus_tag="ACHGNS_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFN6936458.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(38428..38871)
                     /locus_tag="ACHGNS_000478"
     CDS             complement(38428..38871)
                     /locus_tag="ACHGNS_000478"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6936459.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(38887..40047)
                     /locus_tag="ACHGNS_000479"
     CDS             complement(38887..40047)
                     /locus_tag="ACHGNS_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFN6936460.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            40554..42254
                     /locus_tag="ACHGNS_000480"
     CDS             40554..42254
                     /locus_tag="ACHGNS_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936461.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(42356..43189)
                     /locus_tag="ACHGNS_000481"
     CDS             complement(42356..43189)
                     /locus_tag="ACHGNS_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFN6936462.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(43493..45658)
                     /locus_tag="ACHGNS_000482"
     CDS             complement(43493..45658)
                     /locus_tag="ACHGNS_000482"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFN6936463.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            45918..48212
                     /locus_tag="ACHGNS_000483"
     CDS             45918..48212
                     /locus_tag="ACHGNS_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFN6936464.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            48205..48870
                     /gene="pgmB"
                     /locus_tag="ACHGNS_000484"
     CDS             48205..48870
                     /gene="pgmB"
                     /locus_tag="ACHGNS_000484"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFN6936465.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            48863..49870
                     /locus_tag="ACHGNS_000485"
     CDS             48863..49870
                     /locus_tag="ACHGNS_000485"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFN6936466.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            49892..50911
                     /locus_tag="ACHGNS_000486"
     CDS             49892..50911
                     /locus_tag="ACHGNS_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN6936467.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(51192..52559)
                     /gene="nhaC"
                     /locus_tag="ACHGNS_000487"
     CDS             complement(51192..52559)
                     /gene="nhaC"
                     /locus_tag="ACHGNS_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFN6936468.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(52668..54089)
                     /gene="tyrP"
                     /locus_tag="ACHGNS_000488"
     CDS             complement(52668..54089)
                     /gene="tyrP"
                     /locus_tag="ACHGNS_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFN6936469.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(54332..56209)
                     /gene="tdc"
                     /locus_tag="ACHGNS_000489"
     CDS             complement(54332..56209)
                     /gene="tdc"
                     /locus_tag="ACHGNS_000489"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFN6936470.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(56500..57756)
                     /gene="tyrS"
                     /locus_tag="ACHGNS_000490"
     CDS             complement(56500..57756)
                     /gene="tyrS"
                     /locus_tag="ACHGNS_000490"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFN6936471.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(57868..58073)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            58336..59637
                     /locus_tag="ACHGNS_000491"
     CDS             58336..59637
                     /locus_tag="ACHGNS_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFN6936472.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(59706..60305)
                     /locus_tag="ACHGNS_000492"
     CDS             complement(59706..60305)
                     /locus_tag="ACHGNS_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN6936473.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            60559..61440
                     /locus_tag="ACHGNS_000493"
     CDS             60559..61440
                     /locus_tag="ACHGNS_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFN6936474.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            61453..63063
                     /locus_tag="ACHGNS_000494"
     CDS             61453..63063
                     /locus_tag="ACHGNS_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6936475.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(63140..63799)
                     /locus_tag="ACHGNS_000495"
     CDS             complement(63140..63799)
                     /locus_tag="ACHGNS_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFN6936476.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVALYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(64082..64747)
                     /locus_tag="ACHGNS_000496"
     CDS             complement(64082..64747)
                     /locus_tag="ACHGNS_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFN6936477.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
BASE COUNT        19457 a        13218 c        11704 g        20507 t
ORIGIN      
        1 ttcctgcata taaatgaaaa tgttcggagt cagccggtgc aatcgtgtga tcactgaatt
       61 gaatatatct aaaagcgcca ctttccttat cttctgcttc gaatagatac cttacgccac
      121 ggtttcctgc ctcgtaggtc aagatttctt tccctacata acgataattt gaacgtttcg
      181 ttgttccatt cttaaacgta aatgccatct ctttctcact gatcttgatt gaagcaatat
      241 ctgtttgata gcccttcgta taatattctt tatattcttg tgctgtctta tcctgattca
      301 atttttcttt atattcaaaa acaggatcaa aagtcccatc ttctacatac gtatctactg
      361 tttgccaagt tcctgcccaa tctgaaagag agcgatcttg tacatcctcg tcttcaaaat
      421 aaccgttgta gacgctcttt tcttttttgg gggtcaaagt atcttctagc gggttttctg
      481 tctctgtcgt ttttttcaac gcttcgaggt ttgcttccat gatagaaaga tagttttctc
      541 cattttctat ttgttcattc gtcaatcctt ccaaaggatt caatacttct aaagatacac
      601 ctgcttcgtt tgctaacgtt ctggcaatac tatctttggc gttttcttca aaataaatat
      661 agttgattcc atgttcttta acaaagtgtt tcagttcagc aattctcgcc gcagacggtt
      721 cttcactaga agaaagacca gcaatcgata cttgattcaa tccatattct aacgctagat
      781 agttgaatgc agcatgttgc gtgacaaaag atctttgttt tgcttgctta aaggtttctt
      841 gataaagctg atggagtgct tgaagtttct cggtataggc tgctgcattt tttgtccata
      901 cttcctgttt ttctgggtaa gcttcaatca gctgatcacg tatgtttgct acctgcttga
      961 ttgctaaact tggagctagc catacatgtg ggtctagctc atgtgtatgg ccttcttccc
     1021 cgtgttcatg gtcatgatca tgtccttctt cacttcccgg aagtaaaagc ataccttctg
     1081 tcgcttttat cgtatggaca ttgccttctt gtaatgtttt ttggattgcg gggacccatg
     1141 tttccatgtt ttcatcatta taaacaaaga tatctgtatc ttggatcttt gccatgtctt
     1201 tagcagaagg ttcataatcg tgggaatcag tccctgctgg aatcaataac tctacttctc
     1261 cttcatcccc gacgattgct tttgtaaaat catacatggg ataaaaggtc gtcatcacgc
     1321 tcaatttttc tttactgtcc tcggctttcc cacttgcccc gcaagcagtt aacatacctg
     1381 ctaaaaccag catggccaat atacctatca atcgtttcat ttatgacact cctcttctca
     1441 tcttctttca tttgaatgtt ccgattcgct tttcgcaaac cgtaatagtt acgttttagc
     1501 aacaggattt aatttatcat atggttaaaa gaactgtcaa gcgttttctt attttaattg
     1561 atgagaaagt ttatatgaat gattgacgat atttttgcaa tcaacctcta tcattttcct
     1621 tttatacccg cttttgtcta taatgaagtt atagataaag gaggaaaaag agtgaaaaaa
     1681 gaaacagata caacgaatcg tttgatccgc tttttaggag gaaaaacctc ttactatgtt
     1741 ttaggaatag ttatcttact tgcgctgatc atttattttt tccatcagat ctcgtttata
     1801 ttcaagcctg tcggaatcat tatcgctact atcttgcccc cattattgtt tgcactgatc
     1861 ttgtattatc ttttggaacc agtcatcaaa cgtttgagca agcacctttc gaggaacttg
     1921 tcagtcatta tcgtttatgt gattatttta tttttattgg ggcttggaag catatggctt
     1981 attccttttc ttgaagaaca ggcaaagaca ttgatcagta gtttgccaga tttgtttcaa
     2041 gattttcaag agagtctccg acaatttttg gagaaaacac cttttgccga ttcatttaat
     2101 caatttttcg cttctattga cgacgtgacg tctgatatag ctggatttat tggaaattat
     2161 tgggaaagtg gcgcacaacg gatcagcaac attttttcaa ctgttacagc tatttttatt
     2221 actttattta ctggtccaat tgtcgctttt ttccttttga gagatcctca aaaattttat
     2281 cagtcagttt tagcgattgt cccaccagca tttcgtacag attttaaaaa tttgacaaag
     2341 attgctaatc aacaattggg ttcatttcta aaggggcaaa tcattgcttc atttatcctt
     2401 ggagcagtct actgggtttg tttcttactg atcggattag aatttgctag tgttttagct
     2461 atttctgctg ggttgttatg tatcattcca tatattgggc cgtttatcgc cttctttcca
     2521 ggtttgatca ttgcattcca agactctact tttatggctg tcaaatttgt cattgtctgg
     2581 tttgccgtac aacttctcca cggtgacttg gtgattcctc gagtaatggg tgatcgttta
     2641 cagatccatc cgattacgat cttaatcgtt ttacttgtca tgggtgatct aatgggaatt
     2701 gtgggcgtta tttttggtat tcctatctat acgttagtca aattactcgt tacttttatt
     2761 ttccgtaaat tcaaacaacg ctacaataaa ttttatggcg ataaaggaga gtatgaaaac
     2821 tcttcatttt ctgaagaaga ttattttaaa taaaacacaa aacgtcgatc ttacgagaat
     2881 agctcgtagg aacgacgttt tgtgtttttg ttttaatttt tcagcaaagc aattgcctgg
     2941 tatggtttta gctccagact ttgatgaaga acagtatcgg aataattatc gataagaata
     3001 ttggcttctg tgaatccttc tgggatggcg atccttgtat ctttatcata gaaatttgtt
     3061 aatacaagca gctgctgtcc ttcaaattgg cgaagaaagc cataaacttc cggatgattt
     3121 gcttcataag catgataatc tccttgtgca acgattggat atgtttttct cagccgaatc
     3181 aatttcttgt aataggacaa aatagaatct tcagaagata gttctttttc tacattaatc
     3241 tcttctagct tgcctccaac ttttaaccaa ggtttccctg tagtaaatcc tgcttgttct
     3301 gtgtctgacc attgcatggg tgttcgagag ttatcgcgag attttgcttt gatccgacga
     3361 aacgcttctt tttctgtgaa tccttgttca aggagtaact tataagcatt caaactttct
     3421 acgtcaatat agtcggaaat cgatgaatag tctgggtcga tcatgccgat ttcttctccc
     3481 atataaatat agggcgtccc acggttcaaa tggatcatgg ttgctagcat ctctgctcct
     3541 tgttttcgat actgatcgat agctacaaat cgattgagcg cacgtggctg atcatgatta
     3601 ttaaaaaata gagcattcca gccgttaccc tcactcatct tttctcccca tgtatgaaat
     3661 agtcgcttta attctgcaaa atcaaatgga ggagtagacc atttattccc attttcataa
     3721 tcgactttca aatgatggaa attaaagacc attgataatt cttctctact aggttctgta
     3781 tacaagatac aattttcgat cgtcgtagag ctcatttcac cgactgtgat gatagtggaa
     3841 tccgtcccaa acgttgcttg gttcaattct tttagatacg tatgggtgat tggtttgtcc
     3901 gtatattcag gtttgccatc attttcttta ttatttttca attgttcgtc tttaccgata
     3961 acattgataa catcgaaacg gaatcctttt accccttttt ccatccaaaa acgaacaact
     4021 ttgaatagtt cctctcttac tgcttggttc cgccagttca agtcagcttg tgtccgatca
     4081 tataagtgca aataatattt ccccgtatct ccaaatggcg accaagcact tccaccaaat
     4141 tttgataccc aatccgttgg ctcttctctt aaaatgaaga agtcctgata atgctggtcc
     4201 cctgctagtg cttttttgaa ccattcatgt tctgtcgaaa cgtgattcag caccatatcg
     4261 agcatgattt ctatccggta tttctttgct tcggctatta gctgttcgaa ttcctccatc
     4321 gtaccaaaga taggatcgat ggctgtgtaa tcagagatat cataaccgtt gtctttttga
     4381 gggcttggat aaatagggtt tagccaaatc atgtcgatgc ctagatcatt cagatacggg
     4441 agtttttctt tgattccttt taaatctcca attccatcac cattactgtc tttgtaagat
     4501 ttgggataga tttggtaaat gactttgtct ttaaatgtca tggatattta cctccattca
     4561 actgatttat tacctaccac aacttatata tacaagttat catttattat tataactgaa
     4621 ccgaaagcgt ttgtaaaatt tatctttata cattctaaga ggtctgttgc ccaataaaaa
     4681 acaagagatc agacatgtat ttgtctatcc tcttgtttaa aatagcaaaa tattcttgtt
     4741 tcttctataa atggtgttca aaagctttta tctacggcaa tcagtcttct caaatgtaaa
     4801 acatgaggag ctaattttat ttgagtattc tttttgctcg atgcaggacg gcttttttac
     4861 aacctctgat tcatggtgtt caatcagctg tccttcggta ttagctcaaa aaacggcaaa
     4921 atatgaaaaa ctattttgcc gtttgtttct ctaatactca ggtcaaaccg ctgatttcac
     4981 aacctctgtt tttattcatt cgatttcaac gcatcaatca tatcgatatg ctttaatttg
     5041 aaatgggtaa caatcatcac aataaccgta aatacgatag ttaatcctgc tgataagaca
     5101 taagcccacc atgtgatcac taatggaaag atgacattct ccatagacgc ttgttgcaag
     5161 atgaagtcag tcaatatata tcctacacca aatcctccga tgattcctaa aagagtaaag
     5221 atgatatttt ctcgaacaat gtacatcgtg acttctttat caaaaaatcc aaggactttt
     5281 attgtagata attcgcgtac acgctcggaa atattgatat tagttagatt atacaagacg
     5341 ataaaagcca gcagtcctga taatacgaca aagattaaga caaccgagtc taagttcgcc
     5401 attgattctt cttgcgtttc gatttgtttg gacatgaaag acgtatttgt cacagcgtct
     5461 gtggcaagca gtttctcgga aagcatttct tcttcttttg ttgtcatctt atctgtacgc
     5521 agcaaaaatg cattatccat catcgattga tcagtaactg aagcataata ttcaggggac
     5581 atataaataa agctacctag atagtttttc acgatttctt ttacttcgac tttccattct
     5641 ttttgatctt catcataaag cgaaatatga tctcctgtag ataagtcaaa tagttctgct
     5701 actttttgcg taatagcgat tcctgtttta ggcaaggagc gtgggttctc ttcaagatca
     5761 gttaaatgga taaaacgatc caaatcagct gttttacttg gtacgatcac tgtcagactt
     5821 tgcgaagcat gtcctttttt tctcaattct acggtttgag tatgaatagc taggatctca
     5881 tttacttttt cttgcttctg cagcatatcc gctgcttttt gacgactatc ttccgtggat
     5941 gaatccaatg taacgattgc ttgatagtca atgatcggtc caaattgttt ggctgaaaca
     6001 gaacttagag aatctttcaa tcccacgcct gcaaccatca gcccggcaca tccagcaatt
     6061 ccgataatcg ccataagcat tcttgactta tatcggaaga gatttcggta actgattttt
     6121 tgattaaagc taaggcgtga ccacagcgga gtgatgtatt caagaaaaat gcgtttccct
     6181 ggcttcggtg ctttcggttg aagcaatgca gcaggccgtt ctcttaaatc tttgaataaa
     6241 acgatcattg cagcgccaaa tgcggcaatg aagaatgcgg cagctgcttg taaaatcggc
     6301 ggccagtcat aaaaaataag cgcactccca atatcatacc gctcgttaga cagctcaaaa
     6361 atgatccttg gcaaaagatt cgttcctaat atcgttccta aaacaatacc tatccctgag
     6421 gctagacttg catagattgc atattttttt gcgatttcaa ctttcgtata acctaacgcc
     6481 tttaatgtcc cgatttccct gcggttttct tctaccatac gtgtcattgt cgtaaaggtg
     6541 atcaatgcgg caataaagaa aaagaaaaca ggaaaaacat tggcaatcgc agcgatacga
     6601 tcagacagat caccaaattc ttgaaaaccg acattttctg aacgttcatt gtataggtat
     6661 gatggtttag ttaaattttc aattttttct gcatttcgat tcaattcttc ctctgcagag
     6721 gccagttcac gttctttctc aactaattgc ctttcttgtt cagcaagttg agcttcttgt
     6781 tgagataatg tttgaacagc gattgttcgt tgcgcttcag gaagctgctg gatagttgct
     6841 gactgttgcg ctaactgttt tttagcatct tctaaccgtg ttcttgcttg cgttgtttgt
     6901 gctttgccat ccgctatttt ttgctgtgcc ggttctaatt cttcgttggc cgattttttc
     6961 agctcttcta agcgttcttc gggtcgatct tgtaaatact tttctgcctt ttcttgattt
     7021 tgctccattt tatctttgta agctttgctg tacgtattca aattctttac gtttgtaaaa
     7081 tcaagatata agacagaata aatatctgtt ttaaaaacgg attcaggtaa atagacaaaa
     7141 taatcgacgc taccatttcc gacgttcgcg tatcctctct ccaaattgtt gatgaacaat
     7201 ggagaacgga caaagcccgt gatggtatac tctttttctt tcaattcatc tgaatcaatc
     7261 tgataaatat caccgatttt atacccttca tcttccgctt tttcatccaa tacaagctgg
     7321 tttggttttt ccgggagttt tccatcagtt aattgtagct tgttctgctt gttatttttg
     7381 ttataagaaa atatttgaat cacttcgttt ttgccagtat tggaataata gatctgtttt
     7441 cctgtttcta cttttgcatc tgggatcttt tctgcttgag caatatcttt ttgtgtcaag
     7501 ccaccagtag aaacgatttg caaatcagaa agtcctgctg taccaacata gtgatccatc
     7561 gttttttgca tgacaggtcc agcagttttg acacctacaa ataaaagtgt tcctagcatg
     7621 acaataagga tgattgcaat gaatcgtcct ttagattgcc gaatttcgcg aaaacctgct
     7681 ttaagatatg ttcttgtttt cataaaatcc ccctaccata caatctcatc aatggatgaa
     7741 ggatgatcat ttaattcaac agaccttact ttagcatcat taatgtgaat gactctatca
     7801 gcgatgggcg ctaaagcaga attatgtgta atgatcaaaa cggtattgcc gtgttgtctg
     7861 ctagcgtttt gcaataattt caatacttgc ttgcccgttt caaaatccag cgcgcctgtc
     7921 ggttcatcac ataaaagaag tttgggattt ttagctagtg cacgagcaat tgaaaccctc
     7981 tgctgttcgc ctcctgataa ttgggacgga aagttattca atcgatgagc taggcccact
     8041 tgcctcaata cctctacagg atcaagcgca tcaggagaaa cttccgttgc caattccaca
     8101 ttttcttttg ctgttagatt aggaacgaga ttgtagaatt ggaaaacgaa ccctacatct
     8161 gttcgtcggt aagcagtaag ctgtttatct gaaaattgcg caatatctgt gtcatcaata
     8221 atgatctgac cttcatcagg tgtatccatc ccacctaaaa tattaagaat cgttgacttt
     8281 cctgctccac ttggaccaag tataatcact aattcgcctt tttcaattga aaaagaaata
     8341 tcgtcatttg ctgtaatggt cgtttctccc attttgtatc gtttgtattc attttttact
     8401 tcgacatact tcactctgat tcctccattt tcacgatatt tctcgctata tctatgcatc
     8461 gaatgcgttt tctcacactt atttatttta ccactgaatt tctctagact aaagaaagga
     8521 atcctttaat ttagttgaca aataaacgat ttatgattta cttacataaa aaacattgtt
     8581 atttgaaaga aggaacatca tgaatgattt aaaggaaaat atgatttttg atttatcaaa
     8641 aactgctttt gtcgcgatcg acatgcaaaa agagatcgta aataacggta cactctctcc
     8701 acacactgca gcatctattc tgacaaacaa tgatttgtta gtaagaacat tgaaacgtac
     8761 cgctgccttg aaagttttag tgaacgtaga catctccact ttcccttatt tgagtcaaca
     8821 aactgatatg ggtggaatga ctgcttctgt acctccagaa ttcactgatt tacttttgaa
     8881 ggattctttg aaagatacag ataatatgtt gactatcaca aaatacaatc caagtgcttt
     8941 ttttggtact tctcttgatc ttcaacttcg ccggcgtgga atcgaaacta tcattttaag
     9001 cggtgtagcg acaacaaacg gtgtatatgc tactgcatta gatgcctttc aacacgggta
     9061 ccatatcgtg cttgctgaag atgcttgcag tgatcgtgac aaagaatccc atcaattgtt
     9121 cataaaaaag atatttccga aaactgcacg agtacgctca acgaaacaaa taatcgaagc
     9181 tatccaacaa agcaaataag tactggcaca cgtgattttc atttacttct ttttgatttc
     9241 atgggaaaat aaccacagga tttagatagg agtatgaaca tggtgaaaaa aaagatcaca
     9301 atcaaagaag tcgctaaact ttccggcgtt tcaatcacaa ctgtctcctt gatattaaat
     9361 ggaaatacgg agaaattcag tccgaaaact gttcagaaag ttttagctgc caaagaagaa
     9421 ttaggctatc agcctgacta ttttgctaga caaatgatta ccaaggaaac gaaaacaatt
     9481 ggtgtactcg ttcctgatat cacgaatcca ttcttcagta cactgatgcg aggaattgag
     9541 gatatcttgt ataagcagaa tttcgtcacg atattatgta atgctgattc tgatcatcaa
     9601 aaagaaatcg aatatctagc cgagctcaca cgtcgaggtg tggatggatt cattatcgcg
     9661 acttctgctg tctctacaga tgcaatcaac gaaaatttga aaaaacaagg ccggcccttc
     9721 attgttttgg atcagaaaaa atctgaagga tttagtgatg ccgtacgaac agacgatttt
     9781 cgcggtggtt acctagcggg catgcacttg cttagtctag gacatcaaac gatcgccctt
     9841 gtctatcctg aaaatccacc ggaaaatgtc cacgctcgga ttgaggggtt taaatctgct
     9901 cttgatgttt accaaatccc tcacgaccaa ctgatcctct tacctacaca gttctcaaaa
     9961 caaggtggct accagatcac agcagaactt cttgattctg ctgcaactgg tgtgtttgct
    10021 ttaaatgatg aattagcttt tggattatat cgcggattag aggaagctgg aaaaagcatc
    10081 cctgaagact acagcattat tggctatgat aatatagata tgtgcgagta tatcaagccg
    10141 aaactgacaa cgatcgcaca accaatcttc gaattgggac aaacatctgc caagttattg
    10201 ttagatcgga tccaattccc agaaaaagaa tgggaagaaa aaagattacc tgtacgatta
    10261 gaaaaaagat tttcaactgc acccttgaaa tagctttcaa ggtgtgctat atttttatcg
    10321 aactattaaa acgttttact aaaacgtttt aaaaaataat tttcccatca aaataaatga
    10381 gatagttgga ggtttgtaaa atgaacacgg ttacagtaat tggaagtatt aatatggaca
    10441 ctacccttcg tttaaccaac atgcccaagc caggcgaaac gatgcatgcg catgagattt
    10501 ttcacgctgg cggaggtaaa ggtgccaacc aagcagtggc agccaaacgt tcaggcgcac
    10561 ggacatcttt tattggaggc gttggggctg acagcgaagg acaacagtta ttagatctac
    10621 tgacaaaaga aaacatcgat acatctggga tagctgaaat ccaaggcgcc acaaccggac
    10681 aagcaatgat tatggtagac gcagcaggag aaaatagtat cttgatccat gcaggggcaa
    10741 ataatgcttt ccatgaacaa gaagtcctaa aaaacaagca actgattaca aacagtgact
    10801 ttattattgc tcagtttgaa agtagcttag atgcgacgat cctagctttt tctattgcaa
    10861 aagacgcagg aaaaacaacg atcctgaatc ctgcaccagc tcgtgaaacg attcctactg
    10921 aactacttga aaagacagat atcattatac caaatgaaac agaaacagaa atcattacag
    10981 gaatcagagt gacagatcat aacagtttgg tcgcagcagc cgaaaaactc catgaattag
    11041 gtattggaac agtaatcatt acactaggca gtgccggcgc tttctatcat acagaaaaag
    11101 aacacggtat cgtccctgct tttaaagtgg atgccgttga cacaacagcc gcaggtgata
    11161 catttattgg cgcattaagc agcacacttc agccagacct tagtaacttg aaagaagcga
    11221 tcctttatgg aaatctagcc tcttctgttg ctgtgcagtc ttatggtgcg caaccttcta
    11281 tcccttaccg cgaagcttta gaacaatcag cagtctaaaa ttaacaatga gaggaagaaa
    11341 caaacatgaa aaaaggaaaa gtaatcaatt cagatatttc acgagtaatc gcacaaatgg
    11401 ggcactttga taaattaagt atcggggatg caggcatgcc tgtccccttt ggtactgaaa
    11461 aaatagattt ggctgtctca aacggtcttc caagtttttt atctgtttta gaaaacgttt
    11521 tagaagaact tgaggtacaa tcgattgaat tggcggaaga aataaagaca atgaacccgg
    11581 aaatattgga aaaaatcaaa ttattgttac cagatacacc gattagcttt atcccacatg
    11641 aagaaatgaa aaaagagttg aataactgtc atgcatttat tcgtacaggc gaaatgacac
    11701 cttacgccaa tattatatta gtgagtggag tggtcttcta atggaaacga caatgaatcc
    11761 tgaacagatg gtactgtccg agcagattca tatgctgatt gaacagttgg gaggaaaaga
    11821 caatattgat tctgtccttc catgtgcgac tcggcttcgt atcaaattga aaaaacagcc
    11881 agtagccaat gcagaaaaat taaaacagca aatcccttcc attaagtatt tcattttact
    11941 cgatagatat gcagaaataa tcatggatca gggattggct cctgcttaca aggaattgac
    12001 gcgtcaaatc gatcattcag ctccgaaaga aaagctaaat ccgatcgaaa aactatttgg
    12061 tttgatatcg gaaatcttta cgccgatcat tccgctgttt gccggttctg gtatattaaa
    12121 aggtctggtt gttttagcga ctacgattgg ctggcttagt gaaacaagcg gaacttatca
    12181 tattttgtct gctgcgtcga atgccgtctt ttattttctg ccaatctttc ttgctttttc
    12241 tgctgccaaa acgtttaaag tgaatggata tattgcggct gtcattgctg cagcactgat
    12301 tgaaccgaat ctgacctctt tactttcaga cagtggcaaa gcagctactg attttctggg
    12361 gattcctgta gtactgatgc agtatacgtc tactgtcatg ccggcgattt taggtatttt
    12421 cttttattct tttgttgaaa aattcctcaa aaaatatatt cgcgaaaata tgcaattagt
    12481 atttgtacct ttactttcat tagttatcac ggttcctttg acgttaatgg tattcgggcc
    12541 tgcgggtgtt taccttggcg aaggtttagc tgccgcaatt acttggatga tggatattaa
    12601 cggaccgatc agtggcgcaa tcatcgctgg tggttggaat attttggtaa tatttggtat
    12661 ccaatgggct gtaaaccctg tcatgatcag taatatctct gcttatgggt tcgaccgaat
    12721 cgttccgttg acaggagcag ccaattttgg aatggctggt gcagctttag gtgtatttct
    12781 gcgaagtaaa cgttcaaaaa cccgttcgat ctccggttct gcttttgctt cgattttact
    12841 tgcgggagtg accgaaccaa ctgtttacgg aattgcgatc ccattgaaaa aaccatttgt
    12901 tgctgcttgc attggtgcag ctgctggtgg agctgtaatg ggatttgccc aagtgaaagc
    12961 gatcgctttt gtatttggta gtctgaccac attgcctgca ttcatttccg gtactttttt
    13021 ctggtactta gcaggactgg ctgttagtct agtcgtagca atgatcacta ctctcgttag
    13081 cgggtttgat gaagacctaa tgtcttatga atagttaatg aaaaagactg aagtatgaga
    13141 tgtttatcac atatttcagt ctttttcttt ttaggatcat tatttttata aatttttgtg
    13201 atactttcca aactttggta taatttacat atgcataacc atttttatcc ccacttgttt
    13261 atccccaaac aaaggtggtc tatgcaataa aatgaacgtc aaagaaaaac gaatgaatgc
    13321 tcgttgctta tttacaaatt gttttattta gaatacacag acagatttat gaaaagaaaa
    13381 agcttttcga cacgattatc tgttccaaaa aatgtctaat gaatgctgaa taagacagaa
    13441 aatcaatgaa aggaaaagga ctagtttatt atgaaaaata aaaaaacgat acgtttgcta
    13501 gctgctactt ggctgattgc tccgtctgtt ttgtcgacca ataccgtatt tgccgtagat
    13561 aatcaaatag ctgatcaaac agtcacaaca gatacgacta catccagctc agctactagt
    13621 gaaacaacgg attcttttac agaagaccca actggaacaa ctaactcaac aacaggatcg
    13681 accgagccaa ctgaaattga tccggctgat ccatcagaaa atgaaaagcc agccgagaca
    13741 tattccctta caattgctcc ttctttgatt ggtaagatcc aactgactgc ttcgacagcg
    13801 gattcccctg aacagcaacc tttggatatt acggaaagtg gaacggtaga aggtttatta
    13861 gcggaaacga aggtaactta cactattacg ccagctgaag gtgaacaaat tgcttcagtc
    13921 gctgttaatg gtgccaaaga agtaaattat gatgcaaaga cctttacagt aggtacgaac
    13981 aatttatcta ttactgcaga atttaaagct ggaccggata cttcaacaga gaaaccaact
    14041 gatcctagtg aagaagtccc tagtacacca gatccggaaa cgccaaacaa accagatgtg
    14101 ggtaataaca acaataataa tggttcaaat aattcaaaca ataataacaa taattcgaac
    14161 agcgggaatt cttcgaatca aacaaaccca tctggatcga ataacaatgg aaatactcag
    14221 agagaaccaa accatccaaa caatggttca tcgaatggcg gaacgaacaa tgcttcatct
    14281 cgtccaaata cagcaaatca aagcggtata gagaatccat cttctgcttc ttctgatttt
    14341 gttgtaaaat caccaattga tgctgtattg cctacaaata caacgaatgt ccaacaagca
    14401 atcgttcgag aagcattcaa gcatcttgga aaaccatatg tatggggagc aaaaggtcca
    14461 aatacttttg actgttctgg tttgacttat tacgtatata tgaaagcaac cggtcattat
    14521 atcggtggct ggacaggcga acagcaatat gcaggtacgc agattccagt aagtcaggcc
    14581 caacctggcg acctcgtctt ctggggacct tcttccggtg ttactcacca tgtaggtatc
    14641 tatattggga acggacaatt tatccatgcg cctcaaccag gagataaagt tcgagtaact
    14701 tctatttcag atttcacgcc tgattttgct gtccgagtaa acttagcagg tttacctgct
    14761 gcttcaggtt cgctgacagg cggctcatct attttagatg gattagatgg ctcgttccat
    14821 ttcagtcaaa accaaacaac tgatcagttc ttgaaaaaga ttgcagatga tgcacaggaa
    14881 atcgggcaaa aagaaggaat ctacgcatca gtcatgatgg ctcaagctat ccttgaaagt
    14941 ggatctggaa acagcttatt atcaagcgaa ccaaatcata acctttttgg tatcaaagga
    15001 tcatataaag gaagcagcgt gaccttcaat acacttgaac aagatagttc cggacaaagc
    15061 taccaaatcc gcgcacaatt ccgtaaatat ccttcttaca aggaaagttt ggaagactat
    15121 gccgacttga tcaaaaatgg actgactggc aatcctgatt tctacaaacc aacttggaag
    15181 tcggaaacaa aagactacaa agaagcaacc aaatatttgg aaggacgtta tgctacagat
    15241 cgccaatatt cacaaaaatt gaacgcgatc atcgaagcgt acgacctgac aaaatatgat
    15301 gaaccgaaaa aagaagcagc aacaactgaa gaagcaaacg ctgaaatcct caatatgtct
    15361 aagcgattcg atgtgccaat caaatggaca acagatggcg tgccaatcac aggattagaa
    15421 ttttcaacga taaaacgcgt caacaacgct tcttccatct tccgtgtcgc ttcaatctgg
    15481 gatttgtgga acaattttac ggcaagacag ataccagaaa caactactcg tacagttaaa
    15541 gctgcaactg ttcctgtact aaccttgtta aggttagaca aatcattctg gcttgatctg
    15601 cctttaaaga aatagaataa attaaaaagg ggatgtgaca ctgtcacact ccctttttaa
    15661 tttattctat ttctttatat ttattttgta ttccgttaca aactcgcgta cttggttagg
    15721 ttccagagtg acatctccaa aactttcaag tcgctccgct cctggcagtt cttgagtctc
    15781 aaatgtgata ccgccatgat ttactaattt attcccgcgc atttccggtc catcttctcc
    15841 aaaatttgct gtaaaaatca cgattcctga agcatctgtt tttacttgga tcgatacttt
    15901 ttcatctggg cttgtcaatt ccgcagcatt tagtaaacca gattgttttt tcaacaaaaa
    15961 tggatgatcg atgccgccca ctatcattga ttggcgaaaa tcactggcaa agacctcacc
    16021 taatttttta ggacgttgga agtcaaatgc tgtgccactt actgaaacta cttctcctgt
    16081 aggcgttgta tctttattga gcgtagcaaa taatcggcta ttcaaccaaa gtatatgttc
    16141 gtcgattgac tgtgctggat caccggttaa attgaaatag acatgatttg taggattgaa
    16201 tactgttttg cggtcactga tcccttggac cgtcactcgc catgtatcat cgttcgtcag
    16261 cgtataccgt acttctacat ctaaagttcc aggaaatccg ttttccatat ctgggctagt
    16321 ctgatagaaa atgaccgatg cttcgttttc accattgatg actgtataat gccactgctt
    16381 ggcttcaaag ctaggtgcac caccatgtaa acaatgacca gtgtccgggt ctgtttttac
    16441 ttcgaacgtt tcatcatcta atttgaatgt gccattcgta atccgtccag ccatacgtcc
    16501 gattgaagca ccgatataag ggtctttttc tagatattct tttgcttcat caaaacctaa
    16561 gatcaactct cgtttttcat tatcgattat tgtttgaaaa tgaacaaccc gtgccccaag
    16621 atcactgaca gacaaagatg tcccattttg attcgtgatc gtaatcaagt gatagttttc
    16681 gccaaaattt tgttcattga tttccatgtt ccttgcaccc tcttttcttt catttgcact
    16741 gattccagta tacatggaaa ccgttttctt aacagcttat tccgtgagta acgagaaaaa
    16801 atttactata ttctaataaa agaccgtgaa aaaaaggttg tgacaaaaat attgtcacaa
    16861 cctctttttc cgaacaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaaccaa
    16921 acgaaaaata tgaggagcta tttttgcttg gctgttctta ttacttgatg caggacagct
    16981 ttttcacaat ctctttttca atacgattcc agtatataac catgtcgtca gtacatagta
    17041 gatgaaatac aatacgaaga atatcgtaaa cggaacccaa atatctttgt atggattttg
    17101 catgaataga ccaaacattt gtaatccgaa taagacatgg actacaccta agattcctgg
    17161 tgctaagaat aacacgccta cttctctacg gatcgattgt ttaagcaacc cgcgtcgtgt
    17221 tcctatcttt tcaagcattt cataacgagc aatgtcactc ttgcttcctg acaagatctt
    17281 gaacatcaaa cagctagcaa gcatggtcaa gaaagctaaa cctaggaaga atcccataaa
    17341 ttcaaaacca gagaatagac cattatacac ttcatacatt tcatatttct ggttgctgta
    17401 ctctactttt tgcaaagatg gattgttcat tttattttga gcaacaagtg tttcgattgg
    17461 tttcaggttt gcttggaagt cttttacctg aactagctgt aaagtggatt gtggcaagtt
    17521 cagttgatca aattctgatt gactgactac cttgatttct ttttcttgct gttccggcaa
    17581 ttcatagctt cgaagttcct cttgtacagt cagttcattc atttttttag caggaacttt
    17641 ttctttcgtg atcttcggcg gtgttccatc ttcatgtttt atcatgatca atggttgagc
    17701 cgcaaattca cttgcattat agtaaatcgt tcccgcatct tctttttgag tataagaagc
    17761 agaaagtgtt ggtgaaagtt ctttgatttt ttgctgatct tctgcccgag gattattcat
    17821 cactagatca tatccagaat tgttttctgt caagtctgca atttcatttt taaaacctaa
    17881 tcctaccgtc aaagcgccca gtgccaaggc aaacagcata gcgaccattg agagcatttg
    17941 tgtatattca cgaatacgaa agtttaactg tgacaatgtg aaattgttca attttttcaa
    18001 tgaaatctgt tctgatttct ttagtaatga taaaaagaaa atgatcacag aatggaaaat
    18061 gaaataagtt cctaatacga tcgtaactaa ggctacccct ataccaaaga cttgataggt
    18121 ccccacgtgt ttcatcatat agtaaccgca gcctagaaaa gcaattccca gtactacttc
    18181 aaggaaaaac aagaatttat gctgcttgat tcttgttggt gtctgatccg cacgaatcaa
    18241 tgctaaaatc ggttttttga caatcgaaaa agcattgaca acagcagcta ataaaaacaa
    18301 aacagcaaaa aacaaaacag tgatcaacaa cccgtgaata ctaaaaggtg taaaatgcga
    18361 aatattgaga tctaactgat ccactaataa tcggtggacg atacttgtca atcctacacc
    18421 gacagctgaa cccactaaag tagctgcgat ccctactaag aatgtttcag aaaaaatcat
    18481 ctgtgcgatt tttctgcctt ttgctcccag catcataaac attgcatagt ctttttgccg
    18541 catcgtcatc aaaaaggaat ttgcatataa aatataaacg aatgtgatga tccctagtaa
    18601 aacagaaccg aatcggaaaa tgataactgt agccgcaacg atcgtattac ttttcaaaaa
    18661 agcttcgttg cttgccattg attcaaacat gtaaaaaata gcagatgcca taactaatcc
    18721 tgagaataac accaagtagt ctcttaaacg gctcttgatc ccagtaagtg ataatttcca
    18781 aagcatagtc aatctccccc tactgttcca agtttccaag aatagatagt atttctcgat
    18841 agaaggaatc acggctttga tctccacgtt tcacttcttg atggataccg ccatccttga
    18901 tgaataaaat acgttggcaa taacttgcag aaaacggatc atgcgtcaca agaagaatcg
    18961 atacatgatc ttttgtattc aattcgtcca tcgtatctaa aagatcccgg gcacttttgg
    19021 aatccagcgc acctgtaggc tcatccccta atagaatcgt tggttgtgtg atcaatgcac
    19081 gtgcggcggc gacccgttgt ttttgtccac cggaaatctc agaaggatag ctttccaaaa
    19141 tatgagagat ggataaacgt tctgcaattt tttggacacg ttgcttgatt tcttttggtc
    19201 tgacgccttg taaagaaaga ggaacagcga tattttcttg tgctgtcaag ctttctaata
    19261 aattgaaatc ttggaaaata aagccaatct cattcgcacg aaaatctgct agttgatttc
    19321 cttttaatgt ggtcacatct ttttgattga ttcggatatg cccatctgtt ggtttatcta
    19381 atgtggataa aatattcaaa agtgtcgact taccagaacc agaagcgccc atgatcccaa
    19441 taaattcgcc tttttctaca tcgaagctga tgccgcttaa tgcttgcgtc tttttctcat
    19501 tgttttttcc gtatactttt gttacattct gtacttcgac tattttgttc atttctttcc
    19561 ctacccttta attagttttt ccagtgctgt tttcggcacg tctttttttt cgacttcttc
    19621 atacgaagtg acacctttat ttttgttcca ggtgattttt aaatatgctc ctttacgaag
    19681 cggtcgagtc aattgtccct tgaagtccag ttctttttcc ttaccctctt tcgtatagcc
    19741 aggcaggata tatctatagt tgactgtttc ttgtccagag tctgattttg aaactgtttt
    19801 tgtaccatct gtcgtaattt gtacatagta ttcttctcca cccataacta cgtaatctgc
    19861 gattttcacc cctgcaagta ccacaacgat gaaaacaagt agaccgatca gaagtttttt
    19921 cactttttcc ccttctctct tactttatat actgattata gaaaaaagcg aacaattctg
    19981 ccttcgaatc aactatcatt cttctttcaa ttttgtcatt ttctttaaaa caactaaaaa
    20041 aagagttcaa gacaataatc cgtcctaaac tccttcttcc tatcttacaa aaagtatgtc
    20101 acttacggtc tttcatgtcg cctgtattga taacaggttg cgttcctttg tctgtaatga
    20161 ttgagacaag taaattattg atcaattgga ctttccgatc atcggtgaaa ttgatttctt
    20221 gtccatcttc gatttgctct aaggccattt gagtgattgt gacagctcct tcaacaatgg
    20281 tttgtcgagc agacaaaatg gcttttgctt gttgacgttg gagcatggca ctagcgattt
    20341 ctgttgcata ggctaaatgg ttcaagcgtg tttcgattac ttcgacacct gctactgcta
    20401 aacgctcttg cagctctttt tgcaactcgt cagacactgc cgtcgtgttt cctcgaagcg
    20461 tcaaatcatt ctcattgaat gtatcatacg ggtattggct agcgatatgc cgaatggctg
    20521 tctcactttg gatttctaca aaatcttgat aatacgctac atcgaataat gcctttgctg
    20581 tatcaacgac tttaaagact actactgctg agatttcgat ggggtttcca tccaaatcat
    20641 tgacttttaa aacagaacta ttgaaattcc gaactttcaa cgaaacagtc attttttgtg
    20701 ccaaaggtat cgtaaggaaa aagccatttt ctcgaatcgt acctaaatat tgtccaaaaa
    20761 acaaaattac ttttgcttga ttaggactga ctacagttgc cgaacttaac aacaataacg
    20821 tgatcaacca aagaaaaata cttaaaaaaa gtgcccaaac tttcgcttgc cacataccta
    20881 aatagaagaa aaacaatcca actaaagcta atacgaccaa tccgacaatc cctgcataac
    20941 cattgatata aaatgctctt ttttctctca tgctacttcc tgcgctcctt tatccttcaa
    21001 taaatatcta atgttacttt tttaattttt ttttcgaata cgcgccatta caccttctag
    21061 ttctaactct ttgtcttcag tgatgtacca accgcttgcc tgtgtccctc cggctgcctg
    21121 cggtaaataa tccaattttg ttcgaaccca gtgcttttct tctgtaaaat agtcttctac
    21181 caagacctct agatccatcc ctgctgaaag aggcaaatct tcccgaacag atttaaagat
    21241 aaatcgttcc ttcttcccat catacttcaa tattcctgta tcgataatcg gtaaagaata
    21301 ataatccagc tctttcttca ccgtcaataa atgtttgaaa acttcatggt acacttgctc
    21361 taattggcga ccttctgaat tgttcgggta attaaccatc actttttctt tgtctggatc
    21421 atagaaggtt tggcttagtt gagtgatata agtatgtaat ctttccattt ctgtttttaa
    21481 cttttctctt tgaccttctt ctaccttcat atactctttc accctgtttt ctttttagat
    21541 attttctttc ttattttact atagttatgt gcgttaagcg atgaaaaaca gcaaagattg
    21601 gaaggatctt tgctgttttt tcttaaaata tccagttgag tacaggatca atctgtttca
    21661 ttgtcctctt gatccgtcgt aatattgatc gatgtgtctt tagcagtgat ctttagcttt
    21721 ttctgtcctt gttctaacaa agtatttgtt attggctggt agtctttttc aatataagat
    21781 tcaccagacg tttggatagt atagccactg ttttccatct catgcaaaga taaattgcca
    21841 ccgtttagga tgacttttcc ttctttgctg atcaaactgt cttctagatg gaagttgctg
    21901 ttttcacctg tgacgatcaa gttatccaca ttggcctcat ctaatcgaag tgagcctaat
    21961 tcatctttcg cagttattgt tcctcctgta aattcattga acgataccca gccatcatgt
    22021 gtcttagtat tcactttttt gattgatact tcgttgatat caattgcact attattccct
    22081 tcgattgtca attcagataa ttcatgatct gcagggacaa caatagtgat ttgtgcactt
    22141 gtgtcttcta aactaatgat tgcacctgtt gattttttga ctgcattcac agtcaacacg
    22201 ccatttttct cctttatttc tggcttttct gtcttatcat aagtcgtttt tatctcaaaa
    22261 tccaatccgc gtatgaccga aactgtttga ttttctgcat ggatgacgat tttgtttgca
    22321 tccttgattt tttttgtctc agtaaccata tcgatcaacc gaggaccatt atcccaagtc
    22381 agactctttt gagcgcctaa tgtataagtt gcacctgcta ggaggccacc agtaagcatc
    22441 agaccaatac cggtaatcaa aaaatgtttc aatctcaatt tatgtgtcct ccttcaaacg
    22501 cccgctgaat gcagatctct ttttgtaata aaacgtcgtc ccatccattt aacaaagttc
    22561 atcatgacat aaaaaagccc ttttgttcct ttcagcaaca gtggcgaaat aaatactgcg
    22621 cttcctgtgg cgaacagacc gacaccgata aagaataatc cactcagaat cgattgtcca
    22681 aacacactga ttcctgagat gatcatgaat aggccaacac ctaagatact gatacataaa
    22741 acatagatac taaaaatgag cgctagtaaa gcaaccaata gtcccccaat aaccaatata
    22801 aaccccagtg ctagtggaat gaggacagga gaagcaaata aaccgataat gattaaccaa
    22861 atcaggcgaa tttgtctttt tggatttttc actaactcgt tttctgcttg ttcttcttct
    22921 gaaaagtaac tgatttttaa ttttaaggca aactgctttg gtgtaccata ttcatcaatg
    22981 atctgatcta atgtttttcc agaatctaaa aaatattcct cataatactg gatcaaatca
    23041 aaacgatcgt cttcaggaat ttcttgaaga tgatccacaa cttcattcaa atattcattc
    23101 atttgtgctc tcctccaata accggtctaa agaatctcga aaaacaatcc attcatcaat
    23161 aatttcttct aaatgacgac ttcccattgg tgtcaacgag taatatcgac ggtttctgcc
    23221 ttcaaaaggt tcatcatagg tctcaagata tccttctttt ttcaatcttc tcaaaaccgg
    23281 atatagtgta gattcactga cagagagact ttcttgtacg atttttgtta aggcataccc
    23341 atacatatct tccttgctta atgctgctag cacagtacca tcaagaagaa cagttggaat
    23401 ctggatgtgc attacgtcac cttctattct caagtgtaat attttatttc aatctatatt
    23461 atacaacata taatatagtt tcaaaatcag tttgtcaaat acaaaaaaac tgactaaaag
    23521 atttgatcct ttttagtcag caaaaaatgg cttctctttg atttttatta taacagtggc
    23581 ataatccaat ttgtccacat ccagctcagt ggcatgataa cgatcattgc aggtatcatg
    23641 tcagctactg ggaacatttt cacgttgatc attctaaaac cggtagcgac catcaagaat
    23701 ccgccgcaag ctttaaaatc taagatcatg tctggtgtag tcaatggata aataaatttt
    23761 gccaataaga acaaaatata aaaaataatg aattggggaa ttgctatcac agaaacgaca
    23821 taccctaaat tacacgcaaa aatagctgct gtgaaaaaat caaggacaga tttagaaatc
    23881 aaaatcgtac tgtctcctgt catcccagaa tctaaacttc catagatccc cgtaccactt
    23941 gcacaaaata gaacaatgac cgtcactaat gtgctgataa attcatcgtg ggtcatacca
    24001 agcttttcac tagggaagat tttagaaata ggtacttgca tcagcgcagc ccccttattt
    24061 atcccattac ctaaatgaac aatcaaacca atcgctgttc ctatcactaa agcaaaaata
    24121 acagccggca tatttttcat cggcgcaatg gaatagattc ccatccccat cgaacacaca
    24181 ccaaaaacca tcgtcagctg tgctttaaaa tgttcactta atttatttcc tactaatccg
    24241 ccaattagtc cgccaaacaa aacggaacct acattgatca aaactccagt tggcatcaaa
    24301 aaactcctct ctttttctac gaagcgaaaa aaatcagaaa aatttctgta attattgctt
    24361 tcgctgcaag aaagattgta acagacagag aagattacac caattcattt tttgtgttaa
    24421 agtattcttt attggaataa gtaggaggaa tatgatggat atccataaat tcgaagtttt
    24481 tcttgattta gccgaaacaa tgaatttcac caaaactgct gatcgacaat tcaccaccca
    24541 aggaaatatt tctaaacaga ttctttcgtt agaaacagaa ttagacgtca aattattcga
    24601 acgcgcccat agaaaaatcg aattgaccga agctggaagc ttattgctcc catatgtaaa
    24661 aaatgtcgta gaacaatacc atgctatgca agaagtgctt gaaacctata cgaaagacaa
    24721 caatctttca ttaaatatct taacgatacc gacaatgatc aattataaag gtttttccaa
    24781 aatcaccgag tttttgaaac gccatcctga atttacagtc cagttgaaag aagtggaaag
    24841 tgtcgaatta acttacaatg acgccaatga acatagtgac acgatccact ttgctagaag
    24901 tttccaggcc ccaccaagtt cgattgagtg gttgcctact gaaacagacg attttgtagc
    24961 agttctacct aaaaatcatc ctttagctag taaaaagaaa ttggatctaa gcgaactgaa
    25021 aaaggagaac tttttgctcc ttggccccac tacgaacttg tatcaacctg tactagatct
    25081 gtgtcgcgaa gctggatttg aaccaaaaat ctcttataaa ggagcaagaa tcgacttgat
    25141 catcaatatg attgcaaatg acatggggat tgctattgtt atggaaaaaa cagtcaagaa
    25201 tctattaaaa gaaaatactg tgattctccc tatttctcca acaacagaga gttatcttgc
    25261 atttactcgt aaagtcggtg agcattcttt ggcttcagat acattttggg agtatttaaa
    25321 tcaagaagaa aactaagccc tacattttct gtccatctat ccacccaaaa aaagagagcg
    25381 acgcatgact ttcgctctct ttcttgcatc atgtccttgt ttcaatgaca cctctaacat
    25441 ttctcgtagt tcaataagaa agcagcgatt tttatgccaa aagaaccgta agaacatttc
    25501 cctaatcaca aaaaaacgac taaaaacccc taaaaacctt caatagaatt tttaggggtt
    25561 ttattctttt tcctattgtt tacttaaccg tatcattgct gtcgttccta ctccttcgat
    25621 actgctataa tcaatacgtc catcatgcag ttctagaatt tttttaacca gtgaaagtcc
    25681 tagcccattc ccagaaattt gacgacttgt atctccttga taaaattttt caaacattcg
    25741 atctctggtc tcttcattca ttcctacacc tgaatcggtc acttccagta caatctctgt
    25801 ggctgtctca aatagtttga tatgaatctg tccattgatt tgattgtact ttatggcgtt
    25861 atccatgata ttcagccaca cttggtacaa aaactcttca ttgcctgtgt aatttactgc
    25921 agcaagttcg atatccaatt ctagctgttc tttctcccat ttaggctgaa aaaaaacgat
    25981 tacttctctg atttgttcat ctaatcgata ctctttcttt tccagttgga tacgctggtt
    26041 ttccagtttg ttcaatttta agatgttctc tgttaattgt gaaagctgcg tgatactttc
    26101 gataatccga tataaaaata tttggcgttc ttcgtcagag agatttgggg cttgtaacaa
    26161 ttgaacatat ccttggattg ttgctagtgg tgttttgaat tcatgggata cactggacac
    26221 aaagtcattc ctcagtgtct cgatactatt gagttcttgg accataacgt tgaaatcttt
    26281 atacagttgc tgtacttctg ctactttctg ctgttcatcc attcgaatcg aaaaatctcc
    26341 cgttgctacc agactcatat ttgtccctag cgcactgatt ggatgcagga tacgccgccc
    26401 aacaaaaatc gatattcctg tacccacgac catactgaac cccgcaaaga cgaataacgg
    26461 gtataacgtt ggttttccag ccgattcagc aggcgagata gaatgtcgag acaataagaa
    26521 aataaaaccg agaaaacaca gcagtgtcac taagatcgaa atgaagacaa tactagcaaa
    26581 atagatccat aattgtgaaa atagcttttt tttcatcttt aaataaccgc cttatagcct
    26641 agtccccgta ctgtgatgat ttgaaaatct tccgtctttt caaaacgggt ccttaaacgt
    26701 ttgatatgta catcgatcgt tcgttcttct gtattcgtat ctaatcccca aatatcatcc
    26761 atcaactgtt ggcgagtgaa gatcttgttg ggataagaca gtaatttata cagaagcaga
    26821 aattcttttt gaggcagaat caacgaatgc tgatcttgta acactgtata agcatctagc
    26881 tctaattttg tttgtccgat tatcaagcta ctgctgtaat tgatctttga acgtcgaagc
    26941 aatgcttcca cacgtaggat catctcattg acatcgatgg gttttgtcat ataatcatct
    27001 gctcctaatt tgaacccgcg tttcttatcc tcaaaatcgg cctttgccgt aatcattaga
    27061 acaggtgtgt caaatctggt gctacgtaat gagtctagca agtcatagcc atccattttc
    27121 ggcatcatga tatctgtaat gattaattct atacgtgtcg cttctagttt tttcagcgca
    27181 tcctctccat tcaatgcggt gatgactgaa aagcccgctc gttccaacac agaacggtaa
    27241 agcaactgta attgcggatc atcttctact actaaaatac gattcattga tctccctctg
    27301 ttttttgaaa aatttctaga ttttttcttg tagttcatct tcagttaaca tttatttcgt
    27361 atactacgat cattgttctg atatacttat tagtataccg aatatttcaa tacgggagaa
    27421 gggtgattat gcttcaatta aaagatataa aaaaatatta taaagtcggc gaaaccacca
    27481 ccaaagcatt ggatggtgtg tctgtcgctt tcagacaaaa agaattcgta gcgattttag
    27541 gtcctagtgg ttctggtaaa acgactatgc tgaatgtgat tggtggacta gataattatg
    27601 attccggtga tatggtaatc aacggaaagt ctactaaaga ctttaaggac agtgactggg
    27661 atgcctaccg aaacaactct atcggtttca tttttcaaag ttacaacttg attggtcatc
    27721 taggcattat tgaaaacgtt gaacttggga tgacattaag tggtgtttct aaagacgaaa
    27781 aacgaaaacg ggcagaagat gcattgcacc gagtcggctt gacaaatcat atgcataaaa
    27841 agccaaatca gttatctggt ggacaaatgc agcgagtggc catcgctcga gcactagcca
    27901 atgacccaga tatccttttg tgcgatgaac caaccggagc gttagataca gaaacaagta
    27961 tccagatcat ggaattgatc caagagttat cgaaagaaaa attagtgatc atggtcacac
    28021 ataatcctga actggcccat caatacgcag atcgaatcat tgaattttct gatggtaaga
    28081 tcttaacaga ttcccatcct cacatcgaac gaccaaaaga tgatcaattt aacttacgca
    28141 gaacaaagat gagtttttgg acagcgttaa agctttcctt caacaatatc cgaacaaaaa
    28201 aaggacgtac tttcttgacc tcctttgctt ccagtattgg gatcatcgga atcgcgatcg
    28261 ttctgtccct ttcttctgga ttccaaaaac aaatcgataa cacgcaagct gaaacgatgg
    28321 cgaaatttcc gattactatt tctaaggtaa cgactaacca gactcgtgac gatgccggat
    28381 tgggtgcaag caaagcggat tatccagaca gcaaaacgat tactgcaaaa gtcagcgaag
    28441 aagataaagc acagcacacg aataagattg atcaaacata cgtcgattac gtaacagaca
    28501 tcgatcctaa cttaagtaat aatatcggat ttacccgtac tacaggaatc aatcttttac
    28561 gtgatgtaaa tggcaaagta cagcctgtca gcttctcgaa tcaaaatcct gatacggaat
    28621 ctctttcttt atctagtacg atgtcagcaa tgacaggtgt gggagtgtca agtttcccta
    28681 cacagcttga cactagcaaa gaaaactttt taaaagacaa ttattcttta cttgccggaa
    28741 gttatccagc ctcagcaaca gatgtcgtgc tgatcgtaga cggcaataat aatactaata
    28801 tcaacgcatt gaagaatctt ggatttgatg tgaaagaaga cgaaaaactt gattttgatg
    28861 atatcgttgg tacaacattc aaattagtaa acaacaatac ttactataca aaacttccaa
    28921 ctgggaattt tatccctaat acagattatg atgcgatgta tcagaatgct tctgatgaat
    28981 tgaagatctc ggggatccta cgcgtgaaat cttcttctac aatgaatctc ttatctccag
    29041 gaatcgctta tagcgatcaa ttaacgactc aaatcgtcaa cgaaaacaaa gaatcagaaa
    29101 tcgtcaaagc tcaaagagac agtggtgtaa atgtcttaac tactgaaaaa gttgacgaaa
    29161 atgccaaaca aacattgctt agttatttag gtggagacag cttgccttca agtatcatga
    29221 tttatccgaa caattttgaa gacaaagaaa agatcttgga ttatctagat gattacaata
    29281 aaggcaaatc agatgaagat aaaatcattt atactgattt agctggaacg atgactgaac
    29341 tgactggcgg attgatggat gctatcactt atgtccttat tgcttttgca gggatctctt
    29401 tagtaacaag tatgatcatg atcagtatca ttacttatac atctgtgatt gaacggacaa
    29461 aagaaatcgg tgtactaaaa gctttaggtg cacgtaaaaa agacatcact cgcgtatttg
    29521 acgcagaaac gtgtatttta ggtatttctt ctgggatatt aggtgtgttt attgcatggc
    29581 tagctacgtt ccctatcaat agcatcttat acaacatgac ggatctaaaa aatgttgctc
    29641 aactaaatcc tgtccatgcg attattctag tgatcgtctc tactatattg acgatgcttg
    29701 gcggtcatct tcctgcacga atggcagcga aaaaagacgc cgctatcgct ttgcgtgcag
    29761 aataaggttg tgacaaccac atttagattt gagtcataag agcgctgaaa tagtagaaaa
    29821 cagcaagaaa cgaggaagta atatgttcct tgtttcgcgc tgtttttttt aattattttt
    29881 tataattctg gtatactttt ctttcctttg taccacaact cttctggcgt atacctgtct
    29941 tttccattta tgaatactgt tccatctgct tcaatcgtta cttcagtccc aatccacata
    30001 ttacgagaaa gtgcgagaag attttttctt tttcctcttc ctttttttcc aaattgcaag
    30061 gcatcggtta tctcttctgt ccaatgagat ttcacgtatt ttacttctcc gttttcaaat
    30121 gtaactttca aaatattcgc tatattttcc acttttactg ctttaggaaa tgactcctcc
    30181 atttttttca ctttccttcc tccaacacta agtatgcgct ttcttttagt gataagtata
    30241 tcatagttta ttctattatc aaaaaaacgc atccattcca ttgtagaata ctttaccgaa
    30301 caagttgaat tgttttcctc tttttcacct tttactatag ataggtaaag acaactaagg
    30361 aggaatctct catgacagat caaccgtatg atttaagaaa agtcatcgaa gaactaaaaa
    30421 cacttccagg tcaatatcac gaaacagata ttgaaatcga tcctgaagca gaattatctg
    30481 gtgtttaccg ttatatcggt gctggcggta cagtaaaacg tccgactcaa gaaggaccag
    30541 ctatgacttt caacaatatc aaaggtttcc ccggcacacg tgtgaacatc ggaacaatgg
    30601 ctagtcgtaa acgtgtaggt gaaattttgc accatgatta tcgaacactt ggtcgtttct
    30661 tgaaagacgc tgtagaaaat ccaatcaaac ctgtgaaagt agacaaagca gacgctccag
    30721 cacaagaagt cgttcatcta gctaccgatg cagacttcga tattcgcaac attttgcctg
    30781 cacctacaaa tactcctgaa gatgctggtc catacatcac aatgggtgta gtcttaggat
    30841 cagatccaga aaaaacaatg agcgatgtaa cgatccatcg tatggttcta gaagataaag
    30901 atacgatcgg tatgtacatc atgcctggcg gacgccacat tggtcacttc caaaaacaat
    30961 tcgaagcctt gaataaacca atgcctatca ctatcaatat tggtttagat ccagcaattg
    31021 ccattggcac gactttcgaa ccacctacca ctccacttgg ctacaatgaa ctatggtgtg
    31081 caggagcttt gcgtaacgaa cctattcaaa tcgttgatgg tatagcagtc gatgaagtgg
    31141 gtattgctcg tgctgaattc atcattgaag cagaaatcat gccaaatgaa acaatgcaag
    31201 aagatatcaa cacgaactca ggaaaagcaa tgccagaatt tcctggctat actggggatg
    31261 ctaatccagc tgtacaagta gtgaaagtca aagcagttac tcaccgaaaa gacaatccaa
    31321 tcatgcaaac aacgattggt cccagtgaag aacacgtatc tatggccggt attcctacag
    31381 aagcaagtat tcttgccttg gtcgataaag ctattccagg aaaagtagta aatgtctaca
    31441 atcctcctgc aggtggtggt aaattgatga ccatcatgca aatccgtaaa gaaaacgaag
    31501 cggatgaagg tattcagcgt caagcagcta ttcttgcttt atcagccttc aaagaattga
    31561 agacagtcat tttagtcgat gaagatgttg atattttcga catgaacgat gtagtttgga
    31621 ctttcaacac tcgtttccaa ggagataaag atatcatcgt gatcccagga atgaggaacc
    31681 atccattgga cccatcagaa ttaccagaat ataatcctgg ctctattcgt ttccgtggga
    31741 taagtgcaaa aactatcttt gacggtacag ttcctttcga tatgaaagaa cattttgtac
    31801 gtgcaaaatt ccaagaagta gaagattggg aaaaatattt gaaataatga atgatctgca
    31861 actgttttga catcataaaa aggagtgcgg aaaatggaac aattggcaca acggaagaaa
    31921 aaaatcgtga ttggtgtaag tggcgcttca ggcaccattt atgccatcaa cctagtgaag
    31981 aaactaaaag aatatccgat gatagaaaca cacggaatca tcagtccatg ggcaaaacag
    32041 aacttaaagc tggaatccga cttatcacta actgaattcg aaagcctgtt tgattaccat
    32101 tattcaaaca aagatttagg tgctgctgtc gccagcggat catttttgac cgatggaatg
    32161 atcatcgtac cggcaagtat gaaaacagtc gcaggtatct ctgttggttt aggcgacaac
    32221 ctgattgcac gtgctgctga tgtaacaatg aaggagcaac gaaagttagt cattgttcct
    32281 cgtgaaacgc cattaaatgc cattcacttg gaaaacttga ctaaattagc acgactaggt
    32341 gtgcaaatca taccaccaat tcctgctttt tataatcagc ctcaaacgat ccaagatctt
    32401 gttaatcacc atacaatgaa acttttggat caatttgaaa ttgtaaatga gcattcaaaa
    32461 cgttggcagg gtgattaata tggaaaaaga aacgatagtt gaagtaacca aactaggttt
    32521 cacgatccaa gctgctgtta aagtaatcgg taaagactta ttgatcactt taacaggcgg
    32581 agatacacca catatcggaa cagtcactac attttctcgt gatacagaga tgcaaactgt
    32641 acgtttccct agccacgatg gtcggttcca taaagacgat gtcttagccg aaaaaatagc
    32701 tgtgatcatc aaaactgccg ttccgggaag ttgtacgatc acttccggta tccatgttga
    32761 ccatatctcc aatgcacaaa tcaacgcctc ttttccgatg tcagaagagt taggttacaa
    32821 gattttagat tggttggaaa aaatagattt ccatatcaaa taaccagttt attataaaga
    32881 tggtgaaaaa ccattatgaa agaagaggtt gtgaaatcag cgttatgacc tgagtatgag
    32941 agaaaaaaat ggaaaaatag cttctcatat tttgccgttt ttgagctaat accgagggac
    33001 agctgattga acgccgttta tcaagaggtt gtgataaagc cgcttcgttt cgagtagtaa
    33061 gaaaaatttt gtaaacatgg gtttccacat ttttgcataa atttgactta ctaccgagaa
    33121 actggctttt gaacaccgtt tatcaagagg ttgtgacacg tcttttgtca caacctcttt
    33181 tatttttgct tcactgtaac tgctacaata tcaccgattt cttttccgat gtttttacga
    33241 atatcttttc gtagtccaag gatgtagcag actgaaccat ctggattttt cacgcccata
    33301 ttcacgatac tgccttcata cggcacattg tcaaaagttg cttggacttt aactcttcca
    33361 gcaccgaatt cttttcttat atcatacgga aaaataatat atgccccgcc cttgccgact
    33421 tcggaagcat agatttttga ttcaaaagaa taaatttccc tcattttatt tctcctattt
    33481 tttacatcta gtatagcata gcaaaaaaaa gaaggaactg tgacaaaagt cttgtcgcag
    33541 ttcctttttt cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatagt
    33601 caacaaacat gagaagctgt tttttgaaat tgctccttgt gactcagagt taaacggcag
    33661 gctcacaacc tcttccttaa acggtgttca aaagcttgca tctacggcac taagcccaat
    33721 cagatgaaaa atatggagtg ctatttttat ctgattgttc ttactgcttg atgcagagca
    33781 gctttttcac aacctcctac atggtgttca aaatattatt cgccttttgc ttctttcact
    33841 aattttgcga atgcttgttg ttggaattca gcagctaagg ctttcgtacg ttgttcttgc
    33901 tctgctttgc tcatttttgg attttcagtc attgctttta ccataccgtc ttgaacataa
    33961 gcttttgctt cactttccat cgtactttgc tgtttcttca ctaattcagc taacttggct
    34021 gcttggtccg tacttaattc tacccatgtc tctgggattt tgaatgtatt cactcggctg
    34081 tcatattcgt gattgttatc agcaatattg aaccagaatt tatacatgtc gtttttgtat
    34141 tcccaataag tagttttcgt cactttttca gcatcgccgt ctgtcttttc tactttattt
    34201 gtttcgttat ccgtaccagt tgcttttggt ttttcttgtt tttcatttgt tcggtaaaga
    34261 tacacttttt cttttccatt ccctaaagct ttatacaaaa gcatatctgc tccttgggaa
    34321 tcagcactgg aaacaatttt ttctgttttt gtttccgtta ctttttccat tccaaagtga
    34381 ttggacaaat tagcgacgat caaacttagt gaagcgacga agattagtcc gaaaatcaaa
    34441 gataaaaagg tttgccatgt ttttttagcg aacacgttcg ttaaagcaaa agcaagcaag
    34501 ctgacaatca aaactccaat aatcatgcat tatcccccgc tttcattaga acaggttctt
    34561 ttttcttcaa taaaaaggca ataataaagc caagaatccc aaagatgatc gctacgataa
    34621 acgcggcatg gtaacctgac agtgtggcat tgattgcctg atctttataa gctaatggcg
    34681 tattatccaa tacagatttg cttgggatat ttgcttttgt cacattcgtc aacacactga
    34741 tcaaaatagc tgtaccaatc gagcttgcga tttgacggaa tgtattattt accgctgtcc
    34801 catgactgat caagttattt ggcaatgcat tcataccaga agtcgttacc ggcatcatga
    34861 ccatggagat cccaaacata cggatcgcat aaaggatcac gatatataaa actggagtcg
    34921 tttttgtcaa gaaagcaaat ggtgcagttg ccgctgtcaa aatgaacatc cctgtgatgg
    34981 ctaaacgttt cgcgccgtat ttatcgaaaa tcgcacccgt cacaggcatc atagccccca
    35041 tgatcaatgc acccggcaac aacatcaagc cagaatggaa tgctgattct ccacgaatat
    35101 tttgaatata caatggtacg accatttctg cccctaccat cgccatattt gttacaccag
    35161 ctaaaacagc tgcaatcgtg aatgtagatg atttgaatac acgaagttcc aagaatggat
    35221 gttccaaatg taattgacgc catgagaaca aggcgatgaa aatcacacca acaactaaga
    35281 aaccaattac tttcaagctt ccccagccgt catctcctac actagaaaat ccgtaaagta
    35341 aactaccgaa tccaatcgtt gataaaatag cggataaaat atcaatgcta ggatttgaca
    35401 atggaataac acttttcatt aggaagaatg ctaaaaccaa gacaagaaca acgattggca
    35461 gtaccatacc aaacaagtca cgccaatggt agttatcaat gatataacca gataatgtag
    35521 ggcctaaagc aggagcaagt ccaataacga tccctgacat ccccattgct gatcctcttt
    35581 tttctggcgg gaaaatagat agcatgatat tttgcaataa tggcatcgat actcccacac
    35641 cgcaagcttg gatcaaacga ccgattagta atgtactaaa attaggtgct gtaaagcaag
    35701 taatcgttcc gatcaaaaag atcgtcatgg cagaaagata taaatttctt gaactgaatt
    35761 tattgatcag ccatgctgtt attgggatca tgatcccgtt gactagcaag aaaccagtcg
    35821 ttaaccattg cacatcagac gctgaaatat cgaaagcttt catcaatgta ggaaaagctg
    35881 tcgtcaataa ggtctgattc aaaacggtac aaaatgtacc aattaataag acaacaacta
    35941 aaagtgtccg attatatggt tttccgtaaa tatctacggg ttggttgtta ctcatatttc
    36001 tccttctttc tgaatttttt ctgaaactac tagaatactt tagtcctaaa ttcgctattt
    36061 gtcaacctac ttgacacata agttaatacg gatataatta agggaagcaa gaggacaaaa
    36121 ggagtgacaa aaatggttgg ggcttccctt aaacgtttgt tggaaaatga tcaattggtt
    36181 gttggcatca gtgaattaag tgatatcgta ggtgtgtctc cacgtcaatt aaggtattgg
    36241 gaacaaaagg gattcattcg ttcgatcgaa gctgatgcga attgtcctag aaaatatcgt
    36301 ttacctacag tcataaaagt agaactgatc aaaaaatatt tagatgacgg atttactttg
    36361 tcaaaagctg cagaaaaggc agaaatccgc caaaagaaaa tgcaccatgt ccgtaaagtt
    36421 ttttcaaaag caatcaaaga tatcgaattg atcgacgaac gctatacgat tttacaaatc
    36481 ggtaacttca aagaagagaa tgctaagatg tacatcattc atgacaacga gacagacgaa
    36541 ttaacttaca agctccttcc tgtcgatgaa aaagctgact acgaacagtt gttaaaagaa
    36601 ctctaaacgc cactaatctc ctaagtacca agctttttaa taagtagatg ctacataaat
    36661 acttgaaaaa aggcctgttt gaacataaga atatccgaac ggtgatctgc gtttctttca
    36721 tatagaaagg aattctccat cactgttcgg atattttttt atttttttcg gttactttct
    36781 tctaatacat ctgttagcca atttgcatat tctgtttccc gattgatcgc atgcgctaaa
    36841 atcaggtagt gtccgtaatt ttcatgtttt tctttttcag taggaaagat cgtttttttt
    36901 cgctctaata gatgaagtcg tttttcttca tgaaggtcac gctgctgctg aataatttct
    36961 actaatgccg gatcattcac gtctttgacg aaatacaatt tcagaacaaa ttcgtctcga
    37021 ttaaccggta gttcattggt gggtgtatga atccactctg ccaattgttc cttcccttct
    37081 tctgttaatt cataaacttt cttttccaat ttttcacctg tgatctctac ttgatgctga
    37141 atgattcctt gctcttccat ttttgcaagt tcaggataaa tctgactatg ttttgcttgc
    37201 caaaattctc ctatttcatt tttgaacgat tgattcaatt catatcctgt catcttcttc
    37261 ttacttaata accctaacaa aatatatcga agtgtatttt ttcttgccat cagttttccc
    37321 tccttgacaa atctttaaat ccattctatc ataaatgagt aatcgaaaac atgtagttta
    37381 ttacatgttt taatttataa ggagatgctt aaacatgaaa gaatttaaag aattggaaga
    37441 ttttattgga actcacttta tctatacgta tgacaatggt tgggaatatg aatggtatgc
    37501 caaaaatgac catactgttg attaccgtat ccatggcgga atggtcaaag gccgttgggt
    37561 aaaagaccaa gaagctgata tcgttaaatt gacggacggc gtattcaaaa tcacttggac
    37621 agagccaaca gggacagacg tggcattgga ctttatgcca aatgaaaaga aacttcacgg
    37681 aatgatcttt ttccctaaat gggttgaaga acatccagaa atcactgtta ctttccaaaa
    37741 cgaacatatc gcagaaatgg aagcagctcg tgaaaaatat gaaacttatc caaaattact
    37801 tgttcctgaa tttgctacga tcacttatat gggtgatgca ggtatagaca atgaagatgt
    37861 tattagcgaa gaaccatacg atggaatgcc agatgatatc cgtagcggaa aatacttcga
    37921 tgaaaattat aaacgagtaa aataaggttg tgaaagagag gttgtgaaat cagcgttctg
    37981 acctgagtat tagagaaaga aacggaaaaa tagttcctca tgttttgccg ttttttgagc
    38041 taataccgag ggtcagctga ttgaacaccg tttatcaaga ggttgtgaaa tcagcgttat
    38101 gctttgagta ataagaacgc tagttagaaa atagcttttc atattttcta ggctagtggg
    38161 cttattaccg agaagcagcc gtttgaacac cgtttatcaa gaggttgtga aatcagcgtt
    38221 ttgacctgag tattagagaa acaaatggaa aaatagttcc tcatattttg ccgttttttg
    38281 agctaatacc gagggtcagc tgattgaaca ccgtttatca aagattgtaa caactgcata
    38341 ttcacacagt taaattagtg acttcaaaat gaaataatgc tcccagcaaa aaattaggct
    38401 gggagcatta tttcgttatt ctgttcatca aaaatcagcg ttttgattcc atttcatcac
    38461 aagttcctcc tcatttactg cttcatcttt ttgctgtgtt tgaattgtaa aaccttgtga
    38521 aagatagaaa tgaatagctg attgattttt ttgatagact gtcaaagtca gtgttttatt
    38581 ggtttctttt atttttttca ttagttgcgt accgattctt tgttgccggt attccctttt
    38641 cacaaagatt cctgcgataa actgatcaat caccccagca aaaccaataa tttcttgatc
    38701 tatttctgct aagtaaatca ctgcatctgg taaagctttc ctaacagatt ctacttgatt
    38761 ctcccaataa gttgatggaa tgaattcatg agaatccaag ttagcatgca gccaaatcga
    38821 tagaacagat tccagttctt tggcagacaa ttttgttgtt gtgcggatca ttttttctct
    38881 ccttacttat tcattgattt taaaataaaa accatttttt tgaagctctt ttacaagttt
    38941 tcgcccctca aaaaagtata tcttctcttg ttttactttt ctaataccaa aagaaacagc
    39001 aactttagga tcatcagctg aaacgattcg ataagttcca ccaggcaatt gatccagctt
    39061 gactttccca tgatggtcgc tttgtactga aaacggcaca ccatttctag taagtgtttt
    39121 tttcttatgt ttcaaaatga atgaactacc atttaccggt ttccctgacg aatcactaat
    39181 aataaatgaa aaatcaagca cttttttact taatctatac tgatagatta gtcgtccgat
    39241 aaacactaac agtatgacta cagctattat cgttcctgca agaataaacc attgccgcat
    39301 ggttctttcc tgatttcctt ttccttcttc tttttttacc gattcgttat aaggaatccg
    39361 atgtcctgtc actagcaaac gatgcgtgtt gatcatatac ggcgtacatg taagcaaggt
    39421 aactaagtct tctccttctg aaatcgttag aacagacgtg tcatctggca gtaccacttg
    39481 tatattttta acttgataag ccaatttctc ccccaaaaca gtcaaaataa acgtgtctcc
    39541 gttttctaaa cgatctaatt cggtaaataa ccgtcgatta ggtaaaccgc tgtgagcaga
    39601 gatcactgaa tgcgtcgaat ttccaccaag cgggaaagag gttccttgca agacgcctgc
    39661 tccattttca aggatcctac cattgagccg atcataaagt gaaatattta attggatctt
    39721 aggtatattg atacttccga tcagccattc ctctaattgg ctggatgttg tactttcatt
    39781 tctgttcttt tcattgaaag gatcagtacc aggattgaat ccttggtcag ttaatcgttg
    39841 gtttttctcc tccattttct ttcgctgcac ttcaatttca gctgcggtcc gtttttgtac
    39901 ttgttcgata cgtttttgat caataaaact atttaaagaa tccacataaa atggatataa
    39961 agcgatcagt aaccctacga cgatacatat taataaaaac aaacgaaaca aaagtcgacg
    40021 agactgtttt tgcttactct gcttcacatg tgtcctccct tgcaagtttt tttgttcttt
    40081 cttttattat atgggttcag gaaaaaaata gaaaatgaaa gccgatgagg ttgtgaaaaa
    40141 gctgtcctgc atcaagtaat aagtacagcc caaaaaaata gctactcata ttttttgttg
    40201 ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa
    40261 aactgcgtta tgctttgagt aataagagcg ctagtcagaa aatagcttct catattttct
    40321 aggctagtga gcttattacc gagaagcagc tgattgaaca ccgtttaccg agaggtcatg
    40381 aaaaagctac tttgtttcga gtattaagaa aaattctgtt tcaaaacaag gagatgcaac
    40441 aaaacttatg tcgcatcctc tctccttaga actttcactt atgtttttct tgtaaaattc
    40501 ataaatattt tcatgaattt tctaatttct tgcctacctc tcgcatagga tatatgatga
    40561 atttatctaa agtttgcaaa ggagttcgac aattgaaaaa atatggccca tttttagcaa
    40621 tttttttcat tgctttggca tttctcatgc ctcatttatt cacacataaa ctaattttgg
    40681 gtgctgatgc cccattccat tacaatcggt tctatgatgc cgcgcaacaa attaaatatg
    40741 gggatttcca ttattttcct tccctttatg gattccagca aagtggcagg atcgtgaatg
    40801 ccgtttacgg cccatttttc gcttatttac aaggattgat cgtgttgctt gcaggtagct
    40861 ggtttcatta tcagattctt tcaaatttca ttctttatct tatttcagga ttttctatgc
    40921 tgattttact gagatatgtc aatgttcgaa attgggtaag tgtaccagta gccgttttat
    40981 tcatgagtac gtattctatt caatactggt ctatcaacca aggattcact agctggggga
    41041 cttgttttta tcctttatgt atgatcccta ttgctgattt cgtccttcat aaaaagttcc
    41101 ctgttatcaa agtcgctgtc tcagtgggac ttatgacaca gatccattta ctgtcttcga
    41161 tcatgttagc tttgatgtat atcccttttt atctttatta cttttttaac cagactgaaa
    41221 aaggaaaagc actgcttcaa ctgattaagt cggtcttgct ttattttgtt ctgacagcaa
    41281 atatttgggc aggattagct attgtttaca gtgggaataa gattcttcct ccctttgtta
    41341 acagagaaat gagtcaaaag gcgatagatc tggatggcag ttattggctc cattacccta
    41401 aggcctttcc tatattgata ttatcaggtt ttttgctttt tattttcttt caacaaaaga
    41461 acaccttatt ccaaaaaatc gtgttgtaca caagtcttgt tttctttttt ctttctacta
    41521 gcttgattcc atggacgaca ttgatcgaaa aaaatgtgcc ttttattagt ttgatccagt
    41581 ttccatttcg atttttcgtg ccatttacgc tattgcttct actttacctt gccctttcac
    41641 tgaaccagtg gtctgaacaa aagtggttcc gtcttttaag tatatttggc attcttattt
    41701 tttcagcaca gacgatccag aacttatacc agcatctgga aaagtgggaa aatgaaacat
    41761 ttgtcagtcg acatacttat ctattcgata cgccggaaga agcaaggaaa acattcttca
    41821 gcaaagatat gtctaccagc ttgttcgttt tccaaaaaac cacacctgac tatctgccaa
    41881 tctataagga gacaaaagac aataaatacg accggtataa cgaagaaata ctagaaaacg
    41941 aaacaacttt tataaaaaca catccaggcg gtcttttgac aatcaagtgg aaaaacactg
    42001 gggacaaaac tgttcatatc cctgttatcg tctacgatcg gactgtgcta cagcaaaatg
    42061 gaaaaacatt gactgattat gaagtaacag atatcgggac cccaatcgtg aagcagcaaa
    42121 aaggaatcaa cgagctcacg cttcattatc aaacgccaat ctatttctat ttcattcttt
    42181 ctcttacttt gattggctgg tttaccttgc tatgtttatt tatttatcat agatacaagc
    42241 ttttacgtgc ttaggcaaaa gggataaata catccgatca aaaaactctc caaacggtat
    42301 aatcgtttgg agagttttat tttaatcagt ttttttatgt tatatgttat attttttatt
    42361 cgttcacatc taattcgatt tccaccccgt agtgatcact gatgattgga ctatcgatcc
    42421 catcaaaaat ccgttgatat tttttaacct gcattccttc agggacgaaa ataaaatcta
    42481 tacgcaattt ttctgtattt tgttcccaac catctatttt cttttccaca gtagctgctt
    42541 ttcctttttc attcgcgaca acatacgaat cttgaagaag aaggcaactt tctcttacag
    42601 tataataacc ttgagagtct actggattat tgaaatcacc taggaaaaaa agtggtacgc
    42661 gagtttccaa aaagtagttt tccaagtttt tccactcaaa ataaaagcca ctttttttat
    42721 ctgtccacca agaaaagtgg cagctcgaga caaaaatgtc ctgatcatct atctttgttt
    42781 tcccaatcaa gatcttgcgt gtctgataat cttctttctt ttgaaattgg gagacggtaa
    42841 ggacctgact ttccaatgtt tttttcgtaa gcagtgcatt tccttcttca tatttatcat
    42901 aaccgatgtg gctcatttcc caactccaaa aatactcttt tccgtgttcc tttaaaagct
    42961 gaactaaacg gtaggcaaaa ttatctttac ggataggtac ttgattcttt accggacaga
    43021 acttcattag cttcgcaggt tctacttctt cagattccag taattggttg atttcttgta
    43081 aagcaatcac ttcataatct tctaacagga tcttatctgc taattcttgc aattttttct
    43141 ctggttctgg ctcaagccag ctatgcgtgt tgagtgtcat gaatttcatt ggttttctcc
    43201 ttgtcttcat ttcgaaacaa aagggttgtg acaaaatttt tgtcacaacc tctccagcaa
    43261 atagtgttct cctgctaact cctcagtaat aagctcaatg gaccaggagc tattttttgt
    43321 ttggctgttt ttatcacttg gtgcagggca gctttttcac gacctctcca ctaaacagta
    43381 ttcaaactgc tgcttctcgg taataagccc aaccaaacga aaaataggag gagctatttt
    43441 tgtttggctg ttcttatgac ttgatgcagg acagcttttt cacaacctct ccttacattt
    43501 taatggagcc gatcacttta ccagctgtca ctgtacctaa ttgcttgatc actacttctt
    43561 ctactttatc gtttgtaaat acgatcatga tcgttggatc tttttcttcg ttttggatca
    43621 catcaagatc catctcagct aatttcgttc ctgttcctac ttcttggcct tctttgacag
    43681 caatctcaaa agctggctga ttcatttgga ctgtatcgat tcccatatgg atcaaaactt
    43741 cgataccatc tttcgtttcc agcccaattg catgtttgct agggaaaata cttgtcactt
    43801 ttccagcgat cggcgcaaca acttctcttg atgttggaat aacagcaaag ccgtcaccca
    43861 tcattttttg tgagaatacc ggatctttta cttcagataa aggaatcact tctccagaag
    43921 ctgcggaaac tagttcttgt tctataccta agtattgctt actacctgct tgtccatttc
    43981 ctgtagaaac catttctggc tctggaatct ctgctcctga ttgcagcaga tcttcgatat
    44041 ctgatttcaa gacatctgct tttggtccat aaactgcttg gactccattg tctttgacga
    44101 tcaatcccat cgcccctgct cgtttccagg cttcttcagt ccctactttt tctcgatcat
    44161 ttacacttac gcgtaaccga gtcatacaag catcgacatc tttgatattt tcttttcctc
    44221 ccagcaaatg aatgatctgg accacttgct gatctgctgt tcctgttccg ctgttagata
    44281 caacggtttc ttcactgtca ttgtcgtaat ttccgttacg tcccggtgta gcaaagttga
    44341 attttttgat caagaaattt gctaagaaat acgtaacaac accaaaagcg atcacacaca
    44401 acacgaagtt gataagatct ccacctagac ccgctttgat agctaacggt gtccgagtca
    44461 gcaattcgat attcccaaat gaatggaccc gcaatggcaa gatatctgcc atcgcaaatg
    44521 cagccccttg gatcactgaa taaatgacat ataatggcac agcagcaaac ataaacatga
    44581 actccaatgg ttctgttaca cctgttagaa agacagccaa cgctgctgaa aaatacattg
    44641 atttatatct tgctttctta tcaggatcga cattacgata catcgcaaaa gccattccca
    44701 tcaagatacc tgaagaacca atcatttgtc ctactttgaa acgagccggc gtccagttat
    44761 gcaacacaaa ctcatattgc gacatatccc ctgcaccttt aagattcact aaatcagtgg
    44821 cccaagccaa ccataacgga tcttgcccaa atacttgtgt ccccgcctgt gcgcctgata
    44881 aaatctcata cgatccgcca agctgtgtat agttgatagg aatcgtcagc atatgatgca
    44941 atccaaatgg caatagtaaa cgttctaatg ttccaaataa aaacggtgct agaatcggtg
    45001 cgctttcttg tgattctgca atccataaac caaaattatt gattccagcc tgtacgtttg
    45061 gccacaccac agcaagaatc aatgccacga ttgttgacca taagataaca acaaacggaa
    45121 caaagcgctt tccattgaaa aatgacaaag catccggcaa ttttctataa ttatagtact
    45181 tattataagc cattgccccg acaaatccag cgatgatccc tacgaatact cccatattca
    45241 aagctggtgc ttccaataca cttgtaaaaa atcctttaac catgatcttc gtaccgaaca
    45301 gagtgtgggt aaatgcatct tcattcgcaa gcatgtcact tgtgacacca aagatagccc
    45361 cagtgatccg attgattaaa ataaatgaga tcccagctgc aaatgctccg cctgctcttt
    45421 cttttgccca acttcctcct attgctaaag caaataaaat atgaagatta ccaataattg
    45481 cccaaccaat attttcaatt actccgcccg ttgtgacaag cggcgtccaa tcaggattga
    45541 tcaagggaat tgatttccct atactgatca tcaacccagc tgccggcata actgctacca
    45601 caaccattaa agcctttcca aatttttgcc aaaactcaaa actaaatatc tttttcattc
    45661 cgtttagctc ctttacgcaa tcggtttcgt taaacgtatt atagcgtgcg ttttcttttt
    45721 tgtaaatgct ttatttttta tttttctgtc tataccattc tataaaacct ataatacgtg
    45781 tgttcccttc tataattttc tcctaaaaat ttattttttt aagctttttt ctatgaaata
    45841 ttttcttttg tctattgact tgtgcaatcg gttgcgttta ttatgaacgt ataaaacagc
    45901 tcattaggag gagaaaaatg aaacagatca aacgactatt tgctattgat ccatggaaaa
    45961 tcagcacgac acaattagat aaaaaaaatc ttcgactgca agaatcttta acaagtattg
    46021 gaaacggtta tatggggatg cgcgggaact ttgaagaaac ttactcaggg gaccaccatc
    46081 aaggtactta tttggcgggt gtctggtatc ccgataagac acgtgtcggc tggtggaaaa
    46141 atggttatcc agaatacttt ggaaaagtga tcaacgcctt gaactttatc gcgatagata
    46201 tttcgatcaa cgatcagctg atcgacttgg cttctttgga accagaagac ttttactggg
    46261 aattagatat gaaaaatggt gtattatctc gtacattcac aatcactact tctactaata
    46321 aagtacgcat ttccttcgaa cgttttctga gcattgtcaa aaaagaatca gcttatatct
    46381 atttaactat agaaatgctt gaaggtagcg gaacagtcaa actcgtatca aaattggatg
    46441 gaaatgttca aaacgaagac agcaactatg atgaacattt ttggaatgag attgatcgtg
    46501 gaactataga agaaatcggc tatttgacga ctaaaactat tccaaacaac tttgggattg
    46561 aacaatttac tgttactgca gcaatgcgcc atttcgttaa tggaaaatca gtcatccctg
    46621 actatcacga agatacttta gcattacatg gttgcttgtc tgcagaatta tctcaaggtg
    46681 agacatggtc agtggaaaaa gaaattatcg ttcttactag tagagatatt ccagaagcgc
    46741 agcaagcaga aaaagctgtc tcagaattaa cgaaccagac aaacaattat atccaagcaa
    46801 aagaagagca aacagcggct tgggcagaac gatggaagct tgccgatgtg gtcatcgaag
    46861 gagacgaaga agcacaacaa ggtattcgat tcaatttgtt ccaactattc tctacttact
    46921 acggcgaaga tgaacgattg aatatcggcc ccaaaggatt cacaggagaa aaatacggcg
    46981 gagccactta ctgggataca gaagcgtatg ctgtccctct ttatcttgct ttagccaaac
    47041 ctgaagtaac taagaatctg ctgaaatatc ggcataatca actgccgcaa gcaatccaca
    47101 acgctcagca acaaggatta aaaggcgcgc tttatcctat ggtcaccttc actggtgtcg
    47161 aatgccataa cgagtgggaa atcacattcg aggagattca ccgaaatggc gcgattgcct
    47221 atgcaatcta caattatacg aattacaccg gcgatacgga atatttgaaa aatgaaggac
    47281 tggaagtttt agcagaaatc gcacgcttct gggcagaccg tgtccatttt tcaaaacgag
    47341 caaataaata catgatccac ggtgtgaccg gcccaaacga atatgagaac aatatcaata
    47401 acaattggta tacaaatacg atcgcagctt gggtcttgcg ttacacgcga gaaaattatt
    47461 taacttatca aaaagaagct gctatcacta tcagcgaaga agaactagcc aaatggaaag
    47521 atatcgcaga gaacatgtac ttcccagttg acgaagaatt aggaatcttt gtccagcatg
    47581 acacattctt agataaagat ctcatgcctg tatccgatat tcccacaagc gatcttcctc
    47641 tgaatcagca ttggtcttgg gacaaaatcc ttagatcttg cttcatcaag caggcagatg
    47701 tactacaggg gatttatttc ttcaatgata cttttacaaa agaagaaaag caaaaaaact
    47761 ttgatttcta tgaaccaatg actgtccatg agtcttccct ttcgccaagt atccattcga
    47821 ttttagcagc agaattagga atggaaagca aagctgtaga aatgtaccag cgaacagctc
    47881 gcttggattt ggataattac aataacgaca cagaagacgg cttgcacatc acttccatga
    47941 ctggtagctg gttgtcgatt gtccaaggat ttgctcaaat gaaaactgcc catgatcagt
    48001 tgagattcgc accatttctg ccaaaaactt ggaaagctta ttctttccat atcaattatc
    48061 gcggacgttt attgttcatc tcagtcacag atgaacaagt tactttcgag ttattagaag
    48121 gttctcctct agaaatttac gtctatgaac agtcttattc tttagcggat cgtctagaaa
    48181 taacgctaag aaaggaggaa gcaaatgttt aaaggggtat tgtttgattt agacggtgtc
    48241 attaccgata ctgctgaatt ccattatcgt gcttggcgaa agcttggtca ggaaatcggc
    48301 attacgatcg accacacatt caacgaacag ctaaaaggcg tcagccgaga agactcactc
    48361 tctttgcttt tagcttatgg cggaaaagaa cactcttttt caaaggaaga atttgcagaa
    48421 ttagcgaaaa gaaaaaacga ttattattta gagatgatcc agacgatcga accaaaagat
    48481 gtcttccccg gggtcgtgcc tttactggat tctttaaaag aagaaaaaat caagattgct
    48541 cttgcttctg caagtaaaaa cgggccattc ctacttgaaa aaatgggtct tacaccttac
    48601 tttgatgcta ttgccgaccc ggcagaagca acaaacggaa aacctgcgcc agatatcttc
    48661 ctccttgcag caaaagctgt cggactaaaa gcagaagaat gtatcgggat cgaagacgcg
    48721 caagccggta tccaagccat cctctcaagt ggcgctcaac caattggcgt gggacgtgct
    48781 gaagaccttg gagaggagat cccgcttgtc cctgatacga cttttcttac gatcgattac
    48841 ttgaaagaaa agtggcatga ccatggataa tccagagatc tccgtcgtac cagttccaga
    48901 gactgaaata aagaaaatca cattgaagaa cgactactta gaagtcgttc ttctcaatta
    48961 cggtgcccgc ctgcaccaaa tcttcgcacc agacaaagaa ggaaaatcag aaaatgtttt
    49021 attgtcttac gatcgtttcg aagatgttct aacagacaaa agcttttttg gagcaactgt
    49081 cggacctgta gccggaagga tccgggatgc ttcttggggt gagcatagat tggagaaaaa
    49141 ctgcggatca caccatatcc acggtggcac gaaaggctgg tctttccaat tttgggaagt
    49201 agaagttttc aaaactccat catcaatagg tgtggtcttt tacttaaaag atgaattttc
    49261 tacctatcca ggaccaatca ctgctacgat cacttaccgt ttaacaaaaa acgaattaga
    49321 gatgacaacc tctgctagta gccttgttga aaccatctgc aatccgacca accatgccta
    49381 cttcaacctt tcaggaaatg gaacaagaga catctatgaa catcagttaa cggtcttttt
    49441 agatggtgtg cttgaattag atcaagaaaa actgcctact ggaaattgga caaaaaaaga
    49501 aaaactgcca atcgacttta gaaaatcccc aacactgcaa gagatccttg catgttatcc
    49561 agatggacta gatgatgtat ttcttttgca tcatcctagg ctttccaaaa cttccttaca
    49621 attattcgag aagcattctg gtcgccaaat gactatagca acttctaata aaagtatggt
    49681 attattttct acgactggat tcgaagctga tttctctgtc aatggaaaac agatgcattc
    49741 aaactacgga ttagcaatcg aaccgcaaga aatccctgat atcgttcatt tccccaaatt
    49801 tggatcgatc aacctccatc ctggtcaaga acggatcagt cagacgattt atcggttctc
    49861 tgcacaataa caagagaaag gtgtttttat catggctatt accgtaaaag atgtggcgaa
    49921 aaaagccggt gttgccacct cgaccgtctc tcgtgtcatc aacgatcatc ctagtatttc
    49981 cgagacaaca aagaagaaag tccgaaaaat tatggatgaa ctaggttatg ttcctaacat
    50041 gactgcccgc aatctaggaa aacgcatttc cagtgccatc ggcgtaatct taccccctct
    50101 tgattcaaaa gagcggatcg gtaacccatt ttatcttgag atcatggaag caatcaacga
    50161 agaagcgcgg aattatgata tgactacagc aatcgctact gctaaaaatt ttgaattgct
    50221 gttagagaac gtccagcgta tgcatttaca aaaacaagta gatggtttta ttctagtgta
    50281 ttcagatagt caagatactg tcattgatta tttataccaa aaccacatcc ctttcacttt
    50341 gatcggacag ccttatcatc atgagaacga aatcgtttat gtagataatg ataaccagct
    50401 tctaggtaaa caagcaacag aatttttgat ccagaatgga cataagaata tcctgtttgt
    50461 gacaaacacg actcatgaaa gtttgtattt cgaacgatat ttcggttatc aaaaagcaat
    50521 gatgatggct ggtcttcctg cgttatccag tgtagtacta gagcatccgg aggattatct
    50581 gtcctttgag aaaacgttga aagaaaacga agccacagca ttggtggtca ttgacgatat
    50641 cttcgccctt cgcacgatgc agttggcgca aatgtatggc tatgtcattc cagagacact
    50701 ctccgtcatt agcttcaaca actcgatctt ctcaacatta acacaccctt accttacaag
    50761 tatcgatatc gatatctccg aattaggacg tgtcgctatg caaaagctgc gcgaattgat
    50821 ccatgaagaa ttgagcagtg gcgtacgttt ggtcatccct catcgcttga tcaaaagaga
    50881 gacagtcgtt cctttagaaa acccatacta aatagctgac tcactaaata cgctgaccat
    50941 ttatcctatg gtcagctttt tatgtaaaag aagatactac ttctaacaca ttgtttatct
    51001 gtaaaaaaga gcgtgggaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat
    51061 aaacggcgga agcagaagca actccttcgg aaataagctg aaattcacaa aaatttaaaa
    51121 agcaattttc ttgagtttct tcttatttct tggagttaaa cgcttctgtc ccagcctctt
    51181 ttttccttcc ttcaattttt tacacgtcga cgattcaata aaaaaccgcc taatattgta
    51241 aagacaggtg caagaatcga gaaaaaggcg aatggcaagt aatgcaacgc gctaactttc
    51301 atcgttccca taatgaatgt gccgctgact ccccacggta cgagagaatt gacggtcgca
    51361 cctgaatccg ctagtgttcg tgtcaaatag gtttttgata agccaacgtc atcaaaagaa
    51421 cgcttgaatg tttctcctgg aagaatgatg gacagatact gttctccaac gatcagattg
    51481 attccgatac agctcaaggc agtaaacccg actaaacgag aaggtcggtc catcttttct
    51541 tttaattctt gaacgatggt atcaacgatc agatatttga tcagtaaccc acctaatgcc
    51601 aatgcaagta aaatcaaact agcagaacct aacatactca tgatcccgcc tcgagaaagc
    51661 agttcatcta gctttggatc aggtgtattt gcgacataac cattcatcaa gatgtctgct
    51721 gcttttccga ccgtcaaact tttctcatgg ataaaagaaa gaacgagagc agctgtcgat
    51781 cccgctagta atgtagggat agccggaatt tttttgattg cccccccaaa taaaataact
    51841 agtggaatca atgtccaagg agagatccaa aagctcgctt tcatgttttg gatcatttca
    51901 ttgatactag ctagatcggc tgaatgactg ttatgtccta atatcgtata aaaaatcaac
    51961 gaaaggacga atgctgggaa atccgtataa cgcataccag cgatatgttc gaatagatta
    52021 actcctccaa tacccgctgc caagttggtc gtatctgata aaggcgagca gttattccct
    52081 aaaaaggcac cggaaacgac tgcgcctgta gtcattgcat cattgaatcc aagtaaatga
    52141 ccgatcccca aaaaagcaat tccaatcgtc gaaatcgttg taaatgaact gccgacagtc
    52201 actccgacaa ttgcacaaac aagaaatgct gtcggtaaaa agaaacgcac agaaacaagt
    52261 ttaaatccat aaaccataat cgtagggatg gttccagata aaatccatga agcaactaag
    52321 acaccaatca aaagaaaaat caggattgga atgatccctg gagtgattcc tttgatgatc
    52381 ccgtcatgga tctcatccca tgtgaacttt ttaaatctgc cataaaacaa caaacacatg
    52441 aaagctgcaa ggatcgggat atgtggcgag agctggaatc cgatgatcag tactcctagg
    52501 agtgccaaca acacgatcaa tacagcgata ctttctttta aagaaatatt tttattcatc
    52561 gtcgatctcc tatttcaaat gaaaaggccc tgccatgaaa aaactattca cttggcagga
    52621 cctcctacta aatcagcgac aaaacgtcgg ctgaaagact tattttttta tttttcttct
    52681 aaactttttt ctacctcaat cccccaagaa tctttcttga agtgattgat gatcaatgga
    52741 ataacgaaca tgaccactgt gatagctgct actaataatg catcactcaa tgtcgctgta
    52801 cctaatgtag caaatacgac aacgatgata gagaagatca gcatgcagct gactacccaa
    52861 gctaatccgt ttccgttttt accaattcgg taaggacgtt cgaattctgg atgtttctta
    52921 cgcattttaa gcaatgaaac agcaatcaaa atgtacacga tacaataaat aacagttgta
    52981 gcatttgtaa gtgtcaagaa tacagcactt acgtcatcca ttacaccata aagcaaagca
    53041 aataatgaaa taacgattga ttgtgttaga acgacatttc tagatacgcc gtatttgttt
    53101 tctttatgga atccgaattt tggtggaagg aatccttcac gagctacttg gatgattgtt
    53161 tttgatggtc cagtaaccca agctgataat tgtaaaagca caccaataaa taccatgaag
    53221 ctgaagatat ttccgatcac ttctggtaag cctaggattt gacagtcaat caagataggc
    53281 tgcgtgatat ttgctaattc catttttcca tctggtacga catttgctac aagcatcgca
    53341 ttgatgacat tcaataaaac taatccgatc aaagcgataa atacgccttt tgtataattt
    53401 tttgttgcgt ctttcaaacg aggcatgtaa acagaactca tttcgatccc aacaaaaata
    53461 aatgtgatac cagctaagta tttcaatgaa tcaatatgtt ccaagtcagg gaaagctttt
    53521 gaccatgaaa agtcacctag atagccgttt gagactagtc cgactttgaa ggctgccaaa
    53581 acacctaaaa cgaacatgat gactactggg atatataccc ctagccatac accgatgttc
    53641 ccgccgactt ttgccatatc gaatttcaag ttcaagatcg tgatgatcca gtagataact
    53701 aaaatacaac ctaaaacgaa ccagtgatta ttgccaagtt ctacattacc aaatgtgttt
    53761 cctaacaatg gtcctaaagt agaagcgacc attaccatac ctgggaacat ttgcacccat
    53821 aatagccatg caacgacaaa tccccatttg ctgcccagtg ctgtttttac ccataactga
    53881 ggaccgcctt cttgaggaag catcgtggaa agctcaccgg aaaccattga tatcggaccg
    53941 gcaaaaaaca aaactgcaaa aattgaataa aaaatcaatg tccaaccgac tgcggctaac
    54001 gtaggaatac tccgaaccgt tgcacacagt gccattgtca gaccgataaa ggtcccaaga
    54061 cccattttac ttacttcaga tttactcacc gttgacactc tccttgattt tttggataaa
    54121 ataacggtgt ctttgaagta gtttcagcaa taaacgaaac aattcagcga atatttgagg
    54181 ggattctttg agtttattgc ttgaagggtt tcttcaaaga caacctcaaa acaaagggat
    54241 cgaagagctt aggtctcccc ttgctcttca gatccaaaca tgcgtcagaa acagttagta
    54301 ttcaagctgt tttctaacat gattaccttg cttattttgc ttcgcttgcc aataattggt
    54361 ctgcgtagat tttttccaat ttttcttgaa gagctgcttt gattttttca gcatattctt
    54421 caaagtgttc ttctttgttc atgtatggtg tcattacgct ggcacgcaat acagttactt
    54481 ttccggcacg gttccattct tcatctgaga atcctagttg gttcacaaat tgtaatgggc
    54541 tgttgccata gtctgggata gcaaagtcag tatgtgaagt taagaactca ttaccgtaaa
    54601 tgcttccttt cacgtatgaa gaataatcat atacgtcatg gttcaattta ttcattgcaa
    54661 ctaagtcatc gttgcctttt tctttgaata catagtctac catattgaaa tctggataag
    54721 ttaatggatg aacttcgatt tctttgtctc ctactttaaa tgacaagtca ttcaagaagt
    54781 tgtagaaacg atgtgcacct tcgattgatg cacccattaa tttaccatat cctgtgacat
    54841 tcaaaggtaa gacatggtgt gctgcccaaa cgctggctgc tgttgcccct gcttttgaac
    54901 cttccaagat ataagcccca agtaatgctg ggatatctgc gcctttttca aatacatatg
    54961 tcgcaaagta agagataacg tctctcatgc ggatatcttt gatgacgata ccaccggcag
    55021 aatatggaac atagcccatt ttatgtgggt caatcgttac agattctgct tcttcgattg
    55081 ctttatatgc actgtgtact tcttctaaga tataatcttt gttttctgta aagacattgt
    55141 atttgtaatg aacatctttt aaatcttcaa atggaatgaa gttgttgtct tcgtctaaga
    55201 aaatcgcacg gccgtatccg ccataagcag catctacgtg taagtagaag taaataccgt
    55261 ctttttctaa tacgcgacgc aatgcaacga ttttatcgat accgtcgatt gctccttctt
    55321 ctgttgatcc tacaacaccg actacaccta ggattggtgt tttttcagct gctagaccac
    55381 gaacgatttt ttcaagttcg ttgatgtcca tgcgatagtt gtggtcaact ggcactggaa
    55441 tcacttggtc taaaccgata ccgatgatgt cggcagcttt tagccatgaa tagtgtttag
    55501 tttgtggaac caaccatttt cctaattttt ctaaatgttt tccgctacgt gctgagtgag
    55561 ctttgatttc gtcgattttt tctgggacgc tgtctaacag attcatgatt tcttctgttg
    55621 acaagttcat taattcccaa tcgctttttc cagcaactag ttcaggtgtc acttctttca
    55681 ttgcaagcgg caatgatttg atgttgcggg cataccaaag tccttctaag tttgctaaag
    55741 aaccatcagc aacgatgtgg ccccagccat ctttatagct cattaatttt gcaaattcca
    55801 ttcctacttc ttcttccatt tggcttgttg caggagaaga ttcataggca acgttattac
    55861 cattccatag catagcaaaa ttgtacgcta gtaaagatgg catcaatgtt tcagagttca
    55921 tatgacccca gtaacgtccg gcattatgcc aaggaacgga atgggtacgc atccgcgctg
    55981 aaatttctga tagaacatcc tttgttttat tcacagtgtg ttcaaaacta gcactgcttt
    56041 tttcttctgg cgtaataata ggcatgtctt gaggcatgta gttttgacgc cagcctaaat
    56101 gttcatccac caattcattc aacaaagctt tatagatttg gccgttctca gctttgtccc
    56161 caatgaatag ggcatttagg tttagatctt tcgacaatga ttcactcata gtgaaaccct
    56221 cctaaaaaaa tatttaaatt ttgtcgagcc ctacattaca cctgttcttc tattctgtaa
    56281 actaagtttt ggcctgattt ttaatcatat tttaatatgt atcaattatc cgaattgttc
    56341 acacttttga aataattaaa aaaaacgagg gaatttacaa atgtaaactc cctcgcaaag
    56401 attgtattaa cgacaaccag cgttctattt taggatgggt ttcaaaattg gaatgttgta
    56461 atagtatttc cctttttcaa ggtaaaaact tactaaattc taatcaatta ctttggccaa
    56521 aaagtaattt ttcttacctt ttcgaataac cacaaactta ccatcgaatt catcggatgg
    56581 atttatgaca aaatttaaat cggtaacgcg gtcaccgtta atacttatag ctccgtttga
    56641 aacatcttca cgtgcttgac gtttagatgg ttcaattttt gtggaaacaa gcagttccac
    56701 gatattttca ggtgtgcttg aaatttcaac gttcggcatt ttaccgaatc cttgcgcgat
    56761 ttcttccgca tttagttctt taatatttcc agagaacaat gcttgcgtta ttttttgcgc
    56821 ttctttcaag tcttcttcac tatgcacaaa tcttgtaact tcttctgcca aacggcgttg
    56881 tgcttcacgt ttctcaggtt ctgtctctac ttttttcgct aagtcttcga tttcttcttg
    56941 agacaagaaa gtaaagaatt tcaagtattt cactacatcg cgatcatctt gattcaacca
    57001 gaattggtag aactcaaatg gtgaagtttt cttcggatca agccaaacgg caccgcctgc
    57061 agtttttccg aatttcgttc cgtcagcctt cagcatcaat gggatcgtta aaccgaacgc
    57121 ttctgcttct ggtccttcta attttcggat taggtctaat ccagctgtga tatttcccca
    57181 ttggtctgct ccaccgattt gcaattgcac atcgtatgtt ttatgcagat gcaaaaagtc
    57241 gatagattgc aggatttggt aagtaaactc tgtaaatgag attcctactt ccaaacggct
    57301 ggcaacgata tcttttgcta acatcgtgtt gatgttgaag tttttaccgt agtctctcaa
    57361 aaattcaagt aaagagattt ttgacaacca gtcgtagttg ttcacaaatg tgatatttgc
    57421 atctttacca aacaattttc tcatttgatt ggaaagtgcg tccacgttat gttgcacttg
    57481 ttccatcgtt tgcaacacac gttctgtttt gcggccgctt gggtcgccga tcgttcctgt
    57541 tccgccgcca atcagaatat atggatgatg gcctgccaat tggaatcttt tcatcatcat
    57601 aaatggaatc aaatgtccga tatgcatcga atcaccagtt ggatcaacgc cgcaataaag
    57661 tgaaatgctc ttttcttcaa cgagctcgcg aagtctttct tcgtttgttt gctgattaat
    57721 ggcgtcgcgc caagttaatt catcaataat attcatttta ctgacctccg tgtagttttt
    57781 ttatgaaagc aggaacctgc tagcatatgt aattaattat ttcttcagga taggatagga
    57841 aataaaaaaa gtcccttggc atataagcca agggacgaaa tctcgcggta ccacccaatt
    57901 tgtatgtcta gatatccaac atacctctcg aacatttaac gctgtcgcgt ggcaatcact
    57961 gccaaagact cctgaaatgt aattcgcttt aaagcatgta ctggtttgca gcgaccacca
    58021 gctttctgaa acattggctt taacaactac ttcgtttcga tcttcgtctt gttattatta
    58081 atatgtgtct atcatagagc gcaacttttt ctttgtcaac agaaaaagga tatttttttt
    58141 aaattcctca ttttttcttt tttgaatccg ctctaattcc gaacatttta ttttcaatta
    58201 ttgttaaaag aaataacttt ttctgtattt tttctgcaat ttatcgagaa gtcactttac
    58261 tttgcctctt attctttctt cgttacaata aggaatggga caagtaaaaa aatgaaaaag
    58321 caagggacga ggtatatgga aaaaaagcaa aaaattattt taggagccac ttctgcttta
    58381 actatcgtta ttttaattct aagtttgttt tttgtcatcc gatcatctac aaaaaaagaa
    58441 ccaatcattc ctgaaaaaaa aataacagaa aaagcaaaag aaccgacaga tgataagcaa
    58501 caactagtta ctcaagcaaa aaatctggct caaagctacg attatgacca agcgattgcg
    58561 ttattggaaa aagatagtaa gaacggatct actgaaacac aacagcttct tcaaacacta
    58621 aaaaaggaga aagaacaact gatcgactgg tctgatccta ctcagatctc tcatgtcttt
    58681 ttccacagct tgatcgtgga cccgcaaaaa gccttccact ctcaacaggc acaaggatac
    58741 aaagattata tggtgactgt tgaagaattc aaccgttcga tcgaacaact ttatcaaaat
    58801 gattttgttt tagttaattt gaatgatttg atccaaaaag acgaacaagg aaatctttcc
    58861 tttaacgggc ttgcgctacc tgaaggaaaa aaaccattgg ttctttcaca agatgatgtc
    58921 agctattatg aatatatgga taattccggt tttcctagcc gattggtcgt atcaaataac
    58981 aatgaaatca aaaatgtcta taaagagaaa gataaagaag aaatcggtga ctttgacatg
    59041 gttcctctga tcgatgcctt tgtcaagaaa catcctgatt tttcttatcg aggcgcaaaa
    59101 ggtaccttgg ccctgacagg ttacaacgga gttttaggtt atcgaacatc aaaatctcaa
    59161 tatggagaca atgaaaaaac aaaaaaagag attaaagaag ctaaaaaagt ggcggaacag
    59221 ttgaagaaag acggctggag ctttgcttcc catacatggg gtcacttgaa tatgactcaa
    59281 gcttcactag aagatgtaaa aaaagacaat gaattgtggc agcaagaagt tgcacctatc
    59341 ctaggaaaaa caaatattct gatttatcca tttggtgcag acatcagcga ctggcagcct
    59401 tattcagcag aaaatcaaaa atttgcttac ttgaagagtc aaggtttcga tattttctgt
    59461 aatgtcgatg cttctacacc agcttgggga caattaggta cggatttcta tcgaaatgct
    59521 cggatcaata tcgatggcat tcgttttcaa tctgatttag acggaaaaaa tcctattctt
    59581 gatccattca tcaatgtcca tgatgtctat gacaaagaag cacgcgaatc tgcttgattt
    59641 tacgtcattt atgaaccaaa aaagccgaac tgacatttta tcgtcagttt cggctttttt
    59701 atgctttatt tcttcttcgg tgcgattccc ttgatcagga cttctgtcca ttctgctgta
    59761 tatggaagca gttttgcgta tatctccggc ttagaaattc cttctaccat tgcttgaaaa
    59821 tagaggatca agaactcatc cgaatatttc aaatccactt ttccttcttt ccgccctcga
    59881 tggaaaagat caagcatgat actgaaactt tcttgcgtat acgcatccat cgcgtcagaa
    59941 aattcttttt tttctttttt tgtatagtat gtcatcaaat cgatataaaa ttgtttgttc
    60001 actcgattga gataacggat tttgttttca ctcatggcaa tcagtgtttc ttcaaatgga
    60061 agattttccg caatcaacgc tttcgcttct tgccctaaat cattcataaa tgcaaaaaat
    60121 acttcataaa tcagcccttc cttgctgcca aaatatttaa aaatcgtcgc tttacttacc
    60181 tgagcgttct cagctacctt atccattgtc actccagcgg ttcctatgtc tgtgttcatc
    60241 aagtcaaaag ctgcttgtaa aatctctttc tttttctctt gtgctctctt ttcaaaaccg
    60301 ttcatttgta gagcctcccg taattttaaa cttttataat atatatagtt caaatttatt
    60361 ctgaggttta ttataactat ctattgagaa agtatcaagc tagcaattcc ttgtttatga
    60421 attcattgac agaaatttat aaaaagaata ttatgtacta aaataataaa aaaagtttta
    60481 taaaaaggta taaaaccttt ttattaaact atttaatctt gtttagtata tatttttaga
    60541 aaaaggagtg gggtttttat ggcagaaatc gtcaaagtca cacatttgaa aaagagcttt
    60601 ggtcgtttcg aagctttaaa agatgtctct ttcaccgtac attcaggtga agtcgttgga
    60661 tttatcggac caaatggttc tggaaaatcc acgacgattc ggacactttt agggattata
    60721 aaaaaagacg gcggaacggc aacgattttt gggcaagatg tatgggaaga tagtttatct
    60781 atccatgaac gtctttctta tgttccaggc gatatctctt tgtggggaaa tttgactgga
    60841 ggagcgatca tcgatctctt catgaagctt catggaaaag ggagtgctca aaaaaaagat
    60901 gaactgattc aacgtttctc tcttgatcct aagaaaaaag caaaaaacta ttcaaaagga
    60961 aatcgccaaa aagtgggctt gattgctgct ttggctgttg attcggatct ctatttattt
    61021 gatgagccta cttctggttt agatcctttg atggaaacga ttttccaaga agaagttgaa
    61081 aaattgaaag aacagggcaa atccatccta ctttcttctc atattctcag tgaagtcgag
    61141 cgactagcgg ataaagtcgt gatcatacgc gaaggaacac tcgatgaaga aggaacactc
    61201 gatgaattgc gtcatttgac tcgttcgacc atcactatag aaacagaagc agagaatagt
    61261 cacttagcac aattgaacgg tgtccatgac tttgtgcaaa aaggtcacct cgctcaattt
    61321 gctgctgata acgaagcttt cgatgtgatt ttagctgaag cgtccaaatt aggtgtgaaa
    61381 aaatttgaat ctattccacc gactcttgag gatttattca tgagacatta tgaaggatga
    61441 ggaggaagcg aaatgactga taaattttca cgctggtcca cccttttttc tcaaaacctt
    61501 agccgtgact ggaaaaagat cctgatatgg ctagtcggtg tcactgcttt ttctggtggt
    61561 tttgtccctg cgtttgaaga attgacttcc ggacaaggat tagctggatt atacgaaacc
    61621 ttgcaaaacc cggcaatgat tgcaatggtc ggaccaactc cagcttcgaa tgctgctgag
    61681 tatactttag gagcgatgta tgcaaatgaa atgttgcttt tttgcagttt gtttgctgct
    61741 gtgatctctg ttttacacgt ggtgagccaa acgagaaaaa aagaagaact tggtctgatt
    61801 gaacttattc gttcctttcg agtcggacgt caagcaaact ctttagctgt catcgctgaa
    61861 agtattctga tcaatttgct tttagcaata tcgatcagct gtacgatgat ttttttccaa
    61921 gcggattcga tcactgcaga aggtgcttgg ctttttgggc tatctgtagg tagtatgggg
    61981 attttgggga gtgccgttgc actgtttgct gcacaactga tgccaagttc agccagttcg
    62041 aatggtctgt ctttagcgtt tttaggtgga ctttaccttc tacgagcaat aacagatgta
    62101 gctgattcct ccctttcgat gtggaatcct ttaggctggt cttatttgac ctatccattt
    62161 acaactaaca actggattcc tttgctttat ttgtttttgt tcagtctaat cttgataatc
    62221 agttcatttg ttttagagga acatcgagat ttgaacaccg gctatatccc cgaacgtgaa
    62281 ggaaaaccgt atgctaaacg ttctctttta tccttgcgtg gtttattatt ctatctaaat
    62341 aaagggatga caatcagttg gcttatcact tttggtttga tgggggcggc atacggctct
    62401 atctacggga atatgcaaca gtttttagaa agtaacgact taatgaaaca gatgtttatc
    62461 caatctggcg tctcaattga aaaaaacttt acaagcacga tcatggtagt cttgatttgt
    62521 ctagctgcta ttttaccaat tgctagcatc aatcgtttat ttacagaaga aacccacgga
    62581 cgtatgaatc agctgttcgc aacaaaaatt tctaggaacc aactttattg gaccaatatg
    62641 ttactagcta ttctatttgc tatattggca atcctgatta gttctggtgc actagggctt
    62701 tctgctttgg cttcgatgaa ggatgcgaaa gacatggatc tattgatgtt tcttaaagcc
    62761 gggttcaatt tcctgcctgc cgttttagtg tttatcggaa ttagtggctt tctattcgga
    62821 tggcttcctc gaatcggtaa acttgcttat atctacttag cttactcctt tgcactcaat
    62881 tactttggcg gaattttgga tttaccagaa tggttttcca aaacagctgt ccctagctgg
    62941 gttccacgga tgccaaacga ttcttttgat ctttccgtgt tccttacgtt atcatgtatc
    63001 agccttgcac tgttcgtcct tggttctatt ggttataggc gaagagactt actagaagga
    63061 taataaaaaa gcatgtacaa tttcatgcta gcctccaaat ctgggtcaaa aatccaacat
    63121 ttggaggtca tttttttatt tacaagtctt ctaactgctc tttatcaatg atccagactt
    63181 ctcgattctt tctttttaca taaccattct cctctaataa tttaaatttt ctagataaag
    63241 tttcgggggt cgtaccgata aatgcagcaa gttctttcat tttcattggc aggaaaaagt
    63301 gatccttccc tgtcgcttta gctaagtcaa gcagatagag agcgactcgt tcttctactt
    63361 tttccatcat caaaaaagtt gtctgctgct gtacttttgt taatttctca acgttgattg
    63421 ctaaaagttt cagactaagg gcgggataat gcagtaagag ttccgagaaa tctttttgtc
    63481 tcaatatgca aatttccgtt ttttctagtg cttcgccaaa taaagactca tttggaacgc
    63541 ctagcaactg gctctctccc tcatatccgc ccggctctat tactcgtaaa agctgttcct
    63601 ttccgctagc agataattga tacactttca tactgccgga ggccacgatc actaattgag
    63661 gttctccatt cggcgcaaga atctgttctc ctttttccac atagtgatgt ttgacaagtg
    63721 cgtgtacttt ttcttgatct tgctgtacta agtgattgaa caaaggaaca aatgaaacac
    63781 ataaatgatc atgactcata atctctcctc cttctaaaca gctatttcta ataaagaaac
    63841 ataacttccc ttattccaac tgataatttt cttttatcat agcataaacg tcaagcgaac
    63901 cagataccag caaatagagt ggatttcttt ttgtcccttg ctttattttc cttcttttga
    63961 gaccttaagt aattgattcc tatcaatttt ttaaatattt actttttgaa taatcaaaaa
    64021 caaaccttca ctcgcaagcc gagagtaagg tttgtttttc gcattgctta tttcgtgttg
    64081 tctattgaaa tttttccgca cctagctttt gatatagtgg tgctagttct tgtaaggttt
    64141 cagcggttat ttgctcaacg tgttcgattt cttgcggata gaagaatagt ttacccattt
    64201 gaaaggttcg gtctctcatc gcttttttca actcttcttg tttctttgta tcggacaaat
    64261 agtcagacaa agctaagtgg tcgtcaagtt catgttctgg ttgcggccat atgtaataat
    64321 ccttgatatc gactgttttg ctccactctt tcagtatcgc aagcaactgg ttatacgctg
    64381 caatgctacc atatcgttct tcgcttttat aatgttggta catcaaatag atttggtatt
    64441 gtttttgatt caacaaaact gctagacagg cattctcgtc ttgacgagat tcgcttcggt
    64501 acgcgctcca aaaatgactg cgtaaattcc agccattcgt ccagctttcg attttaggtt
    64561 tgctcatgcc gtacatatca ggcaaaagtg cagctgtctg gagctgaatc tctttccatt
    64621 tttgccagtg ttctttatat tctttcttga tttgttcaat cttatctgtt ggatggctct
    64681 ctttgagctg tttgaatgcg tagattggtc gatcaaacaa ctgataatct gattttccaa
    64741 gttccattgt ttcgcctctt tccgttatct tttgtattct acctgaattt tgcgatatgg
    64801 acaaatagag gttgtgaaaa agctgttctg catcaagtca taagaacagc caaacaaaaa
    64861 actgcgatga gcatcaccgc agtttt
//
LOCUS       DAZBTF010000007        62788 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000007 DAZBTF010000000
VERSION     DAZBTF010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.007"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(151..2304)
                     /locus_tag="ACHGNS_000497"
     CDS             complement(151..2304)
                     /locus_tag="ACHGNS_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFN6936478.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2598..3446)
                     /locus_tag="ACHGNS_000498"
     CDS             complement(2598..3446)
                     /locus_tag="ACHGNS_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFN6936479.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3670..5610)
                     /gene="thrS"
                     /locus_tag="ACHGNS_000499"
     CDS             complement(3670..5610)
                     /gene="thrS"
                     /locus_tag="ACHGNS_000499"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFN6936480.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5947..6126)
                     /locus_tag="ACHGNS_000500"
     CDS             complement(5947..6126)
                     /locus_tag="ACHGNS_000500"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFN6936481.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6173..7996)
                     /locus_tag="ACHGNS_000501"
     CDS             complement(6173..7996)
                     /locus_tag="ACHGNS_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFN6936482.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8274..10553
                     /gene="helD"
                     /locus_tag="ACHGNS_000502"
     CDS             8274..10553
                     /gene="helD"
                     /locus_tag="ACHGNS_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFN6936483.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10646..11293)
                     /locus_tag="ACHGNS_000503"
     CDS             complement(10646..11293)
                     /locus_tag="ACHGNS_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936484.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11472..12191)
                     /locus_tag="ACHGNS_000504"
     CDS             complement(11472..12191)
                     /locus_tag="ACHGNS_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN6936485.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12322..13473)
                     /locus_tag="ACHGNS_000505"
     CDS             complement(12322..13473)
                     /locus_tag="ACHGNS_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFN6936486.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13690..14430)
                     /locus_tag="ACHGNS_000506"
     CDS             complement(13690..14430)
                     /locus_tag="ACHGNS_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN6936487.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14434..14757)
                     /gene="rsfS"
                     /locus_tag="ACHGNS_000507"
     CDS             complement(14434..14757)
                     /gene="rsfS"
                     /locus_tag="ACHGNS_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFN6936488.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14814..15407)
                     /gene="yqeK"
                     /locus_tag="ACHGNS_000508"
     CDS             complement(14814..15407)
                     /gene="yqeK"
                     /locus_tag="ACHGNS_000508"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFN6936489.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15391..16041)
                     /locus_tag="ACHGNS_000509"
     CDS             complement(15391..16041)
                     /locus_tag="ACHGNS_000509"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFN6936490.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16051..16362)
                     /gene="yhbY"
                     /locus_tag="ACHGNS_000510"
     CDS             complement(16051..16362)
                     /gene="yhbY"
                     /locus_tag="ACHGNS_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFN6936491.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16378..17487)
                     /gene="yqeH"
                     /locus_tag="ACHGNS_000511"
     CDS             complement(16378..17487)
                     /gene="yqeH"
                     /locus_tag="ACHGNS_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFN6936492.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17484..18011)
                     /locus_tag="ACHGNS_000512"
     CDS             complement(17484..18011)
                     /locus_tag="ACHGNS_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFN6936493.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18191..18976)
                     /locus_tag="ACHGNS_000513"
     CDS             complement(18191..18976)
                     /locus_tag="ACHGNS_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFN6936494.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18969..19838)
                     /gene="accD"
                     /locus_tag="ACHGNS_000514"
     CDS             complement(18969..19838)
                     /gene="accD"
                     /locus_tag="ACHGNS_000514"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFN6936495.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19839..21218)
                     /locus_tag="ACHGNS_000515"
     CDS             complement(19839..21218)
                     /locus_tag="ACHGNS_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFN6936496.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21226..21645)
                     /gene="fabZ"
                     /locus_tag="ACHGNS_000516"
     CDS             complement(21226..21645)
                     /gene="fabZ"
                     /locus_tag="ACHGNS_000516"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFN6936497.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21670..22146)
                     /gene="accB"
                     /locus_tag="ACHGNS_000517"
     CDS             complement(21670..22146)
                     /gene="accB"
                     /locus_tag="ACHGNS_000517"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFN6936498.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22149..23384)
                     /gene="fabF"
                     /locus_tag="ACHGNS_000518"
     CDS             complement(22149..23384)
                     /gene="fabF"
                     /locus_tag="ACHGNS_000518"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFN6936499.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23403..24140)
                     /gene="fabG"
                     /locus_tag="ACHGNS_000519"
     CDS             complement(23403..24140)
                     /gene="fabG"
                     /locus_tag="ACHGNS_000519"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFN6936500.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24152..25069)
                     /gene="fabD"
                     /locus_tag="ACHGNS_000520"
     CDS             complement(24152..25069)
                     /gene="fabD"
                     /locus_tag="ACHGNS_000520"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFN6936501.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25176..25400)
                     /locus_tag="ACHGNS_000521"
     CDS             complement(25176..25400)
                     /locus_tag="ACHGNS_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFN6936502.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25453..26415)
                     /locus_tag="ACHGNS_000522"
     CDS             complement(25453..26415)
                     /locus_tag="ACHGNS_000522"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFN6936503.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26425..26865)
                     /locus_tag="ACHGNS_000523"
     CDS             complement(26425..26865)
                     /locus_tag="ACHGNS_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFN6936504.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            27171..28136
                     /locus_tag="ACHGNS_000524"
     CDS             27171..28136
                     /locus_tag="ACHGNS_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFN6936505.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(28193..28423)
                     /locus_tag="ACHGNS_000525"
     CDS             complement(28193..28423)
                     /locus_tag="ACHGNS_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFN6936506.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(28509..29396)
                     /locus_tag="ACHGNS_000526"
     CDS             complement(28509..29396)
                     /locus_tag="ACHGNS_000526"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFN6936507.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(29485..30708)
                     /locus_tag="ACHGNS_000527"
     CDS             complement(29485..30708)
                     /locus_tag="ACHGNS_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFN6936508.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(30725..31771)
                     /locus_tag="ACHGNS_000528"
     CDS             complement(30725..31771)
                     /locus_tag="ACHGNS_000528"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFN6936509.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(31935..33662)
                     /gene="ptsP"
                     /locus_tag="ACHGNS_000529"
     CDS             complement(31935..33662)
                     /gene="ptsP"
                     /locus_tag="ACHGNS_000529"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFN6936510.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(33662..33928)
                     /locus_tag="ACHGNS_000530"
     CDS             complement(33662..33928)
                     /locus_tag="ACHGNS_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFN6936511.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            34407..36641
                     /locus_tag="ACHGNS_000531"
     CDS             34407..36641
                     /locus_tag="ACHGNS_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN6936512.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            36789..37109
                     /locus_tag="ACHGNS_000532"
     CDS             36789..37109
                     /locus_tag="ACHGNS_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFN6936513.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(37314..37538)
                     /locus_tag="ACHGNS_000533"
     CDS             complement(37314..37538)
                     /locus_tag="ACHGNS_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFN6936514.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(37736..39316)
                     /locus_tag="ACHGNS_000534"
     CDS             complement(37736..39316)
                     /locus_tag="ACHGNS_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFN6936515.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(39717..41093)
                     /locus_tag="ACHGNS_000535"
     CDS             complement(39717..41093)
                     /locus_tag="ACHGNS_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFN6936516.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(41221..42309)
                     /locus_tag="ACHGNS_000536"
     CDS             complement(41221..42309)
                     /locus_tag="ACHGNS_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFN6936517.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            42481..42915
                     /locus_tag="ACHGNS_000537"
     CDS             42481..42915
                     /locus_tag="ACHGNS_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6936518.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(42993..43526)
                     /locus_tag="ACHGNS_000538"
     CDS             complement(42993..43526)
                     /locus_tag="ACHGNS_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFN6936519.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(43620..44798)
                     /gene="mutY"
                     /locus_tag="ACHGNS_000539"
     CDS             complement(43620..44798)
                     /gene="mutY"
                     /locus_tag="ACHGNS_000539"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFN6936520.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(44791..45588)
                     /gene="recX"
                     /locus_tag="ACHGNS_000540"
     CDS             complement(44791..45588)
                     /gene="recX"
                     /locus_tag="ACHGNS_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFN6936521.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            45695..47077
                     /gene="rlmD"
                     /locus_tag="ACHGNS_000541"
     CDS             45695..47077
                     /gene="rlmD"
                     /locus_tag="ACHGNS_000541"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFN6936522.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(47251..47829)
                     /locus_tag="ACHGNS_000542"
     CDS             complement(47251..47829)
                     /locus_tag="ACHGNS_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFN6936523.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            48151..48621
                     /locus_tag="ACHGNS_000543"
     CDS             48151..48621
                     /locus_tag="ACHGNS_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFN6936524.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(48729..49955)
                     /locus_tag="ACHGNS_000544"
     CDS             complement(48729..49955)
                     /locus_tag="ACHGNS_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFN6936525.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(50709..50909)
                     /locus_tag="ACHGNS_000545"
     CDS             complement(50709..50909)
                     /locus_tag="ACHGNS_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN6936526.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(51046..51651)
                     /locus_tag="ACHGNS_000546"
     CDS             complement(51046..51651)
                     /locus_tag="ACHGNS_000546"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFN6936527.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAA"
     gene            complement(51736..52206)
                     /locus_tag="ACHGNS_000547"
     CDS             complement(51736..52206)
                     /locus_tag="ACHGNS_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFN6936528.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(52254..53168)
                     /locus_tag="ACHGNS_000548"
     CDS             complement(52254..53168)
                     /locus_tag="ACHGNS_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFN6936529.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            53314..54117
                     /locus_tag="ACHGNS_000549"
     CDS             53314..54117
                     /locus_tag="ACHGNS_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFN6936530.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            54295..55242
                     /locus_tag="ACHGNS_000550"
     CDS             54295..55242
                     /locus_tag="ACHGNS_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFN6936531.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(55310..55603)
                     /locus_tag="ACHGNS_000551"
     CDS             complement(55310..55603)
                     /locus_tag="ACHGNS_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFN6936532.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYNPNLEEPTYHFKKQ"
     gene            complement(55631..56296)
                     /locus_tag="ACHGNS_000552"
     CDS             complement(55631..56296)
                     /locus_tag="ACHGNS_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFN6936533.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(56436..57068)
                     /locus_tag="ACHGNS_000553"
     CDS             complement(56436..57068)
                     /locus_tag="ACHGNS_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFN6936534.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(57075..58142)
                     /gene="liaS"
                     /locus_tag="ACHGNS_000554"
     CDS             complement(57075..58142)
                     /gene="liaS"
                     /locus_tag="ACHGNS_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFN6936535.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(58139..58870)
                     /gene="liaF"
                     /locus_tag="ACHGNS_000555"
     CDS             complement(58139..58870)
                     /gene="liaF"
                     /locus_tag="ACHGNS_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFN6936536.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(59039..59518)
                     /gene="greA"
                     /locus_tag="ACHGNS_000556"
     CDS             complement(59039..59518)
                     /gene="greA"
                     /locus_tag="ACHGNS_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFN6936537.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(59654..60829)
                     /gene="mltG"
                     /locus_tag="ACHGNS_000557"
     CDS             complement(59654..60829)
                     /gene="mltG"
                     /locus_tag="ACHGNS_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFN6936538.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            61033..62202
                     /locus_tag="ACHGNS_000558"
     CDS             61033..62202
                     /locus_tag="ACHGNS_000558"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFN6936539.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(62469..>62788)
                     /locus_tag="ACHGNS_000559"
     CDS             complement(62469..>62788)
                     /locus_tag="ACHGNS_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936540.1"
                     /translation="GQVIYSSFSPNKDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSK
                     NATVLKQGGPFVTVKSESGINKVTIRYSYTFFSKISLFISIITLIVLLFYCIKLKIRT
                     SHE"
BASE COUNT        18174 a        13181 c        10594 g        20839 t
ORIGIN      
        1 aatttttgtc acaacctttt ttgccgagta aacggtgttc aaaagccagt ttctcggtat
       61 gaagtcaaat ttaagcaaaa atgtgagaag ctattttcac aaaatttttc ttcatactcg
      121 aaactaagcg gctttttcac gacctctttt ttattttgcc atgaacatat cataatacgc
      181 gtccattatt tctttagcaa tcgtcaagtt tatgtggtct tgttcgtcta ataagttcgg
      241 caagacaaca ctgatggcaa tttccggatt gtctgttggt ccgtatgcca cgatattact
      301 attgactgtc gcaatatctg gatgaccttc cgcaaccgtt tctgcagtcc ctgtttttgc
      361 ggaaaggtcc attttcgcac tggctaatgg tttggctgtt gtgtaaggat cggtaccatg
      421 gacaacttgg tggaatcctt cacggaggat cgccatattt tctgcagaaa tattgaccgt
      481 attttctact gttgtgccaa tttctttctt cacttcaccc aagtttccat tttcgtcatt
      541 gccatagatc gctttgacta ggtgcggctg tactcgtttc ccgccattag cgactgttga
      601 agcatattga gctaattgga ttggtgtata tgtgtcaaac tgtccaaatg ccaaatcggt
      661 aaagttacct ggagcatacc attctttgcc attatccgca ttgaacttct tcatataatc
      721 aacagaacgt gagatcccag gagattcatt cggcaaatcg atcccagtcg ttgtaccaag
      781 accaaactct ttgaaagagt ctctcaattg ctgataagct ttagcttctt catctaaact
      841 aggtagtccc attccaggtg tatagtctag tcctaacatc tttaacgcca ctttgatcat
      901 atacgtatta gaagacaatt ccgatgcttt gactgcatca agagcaactt gtccgcttcg
      961 attaaagaca gagctttttt ctgaagcacc ttgcagacga atcggctcat caatcaaaac
     1021 ttgatttccg ctgatcgcgt tacttgccca gcctgctgtc aaagtaccag ccttgactac
     1081 agaccctgga tcaaaggcgc tagtaatcgt gcctaaagca ttctcggtga tttctttgga
     1141 accttgttca tgactgaaac cagtcattgc tagcacttct cctgtctgag gattcatagc
     1201 cactgcataa gcaccaggcg aatactgtgc tttgccgttg ttgatcaatg tttgataatt
     1261 ccttttcaaa atttcttcta cttttgattg gaattctgca ttcatagata acattaaatt
     1321 agaacctttt tccccagaga agatttcttt ttgattcgaa acatttcctt cattatcaag
     1381 ggaaacttca tattgggttt tggttccttg caggacatct tcatattgtt tttccaaata
     1441 actttgtcct acacggtcat tccgagaata accttttgct aggtatttct ctgcttcatc
     1501 tgctggaagt ccttgctttt cagtcgtcac actgccaaga atacttttca gtgaacttgc
     1561 tgcattatat tctcgtgtcc agtctgtgcc agtcgaaact cctggaagtt cagaagcgcg
     1621 ttctgccaca acagctaact cttgatcagt aacaccagag tttttgatat agacagtgtt
     1681 taattcatat gcactattca tccgtttaaa gatggtagca atttttaatt gctcctcgtt
     1741 taaattaatc tcatcagctg tgactttatt gaccatcgca ctatattgtt cgctgttccc
     1801 taattgcttt tcttttgccg aaagtctttt tgtggcttct ttcaaatgtt tgggatcagc
     1861 cagccaaaaa tcttttttat ctctatctgt cagattttca tctaccggaa catcgatcag
     1921 cgagttcagc ttcgttgcga tttcaagcaa atctttagcc gtaacttgtg ttcctctcgt
     1981 atatgtgatg gcctgattcg ctttattcgt aactaaagct tttcctgtgg agtcgtagat
     2041 cattccgcga ggagtacttt cttggaccgt aatcgtagaa gtagacttca ctcgcgcctc
     2101 catctcttcg ccgttcacga tctgcaaata acccaatcta gcaatcaacg caacaaataa
     2161 cgtaaatata atgaaaaaaa gaaaatttaa gcgaaaagga acatgggacc ggcgaaacgt
     2221 tttgttctta ggttcttgtg caccttttct tattttttct aaccaagaag tatttaatat
     2281 ttttttcata aaatctttca tcatttccta tccttccgcc tatttacatt catgcttcat
     2341 tgtacccaaa aacttcttaa taaaaaagaa aaaatgggcg gagcaagaaa ttctttacct
     2401 aatttcaaga taaaatcaac tttttgtttc acagcaatca cttaaagtta catatccaac
     2461 tatttttttc attaaaaaat aaacttattt ttgaaaatta tgttttctta agtcgtgaaa
     2521 cactttgatg gatcatttat ttgtggaaaa ctacgttttg ttattctaaa aaacttatat
     2581 aaaaaatagt aaggttttta tttcgaatcc ataaaatatt cttggattgc ttctcgtgtt
     2641 tttttcactt tttcgctaag cttttcgttt ttagccatat agaaataaaa aatgatatcg
     2701 atcgtagcaa attgggccaa aagtgaatta gttgccgcac ttcgattcgt gatttctttt
     2761 ggtctagatg tttgtacgag tacatcggct atcttgctaa gcgggttatt tccaaattgc
     2821 gtcaacgcaa tgatttcgat attcatgctt ttcgccagct cggctaatgc gactacatct
     2881 gcacttctac ctgagtttga aacaagccaa agcactttct ttttctcatt gctgacaagt
     2941 tgcgggagca agacgtggat atctttttca aaaatgattg gcttaccgac acgagaccat
     3001 ttttgcagaa tatcttctac tactaaagaa gaggcgccaa ccccatataa aaaaacagtc
     3061 tctgccgatt ccaaacactc tacgactttt tggatcgttt cttcttctag aatatctact
     3121 gtttctttta tcgctacttg tgcattgtta ccaagtttgt ttttgataga gtaaaaggac
     3181 tcatttgctt ctatatcact aaaaccttca tcgattccag tctgtagttc tgttgaaatc
     3241 gtgattttga agtccgtcag actttgaaaa ccgatttttt tggaaaaacg tacaactgtc
     3301 ggcgcactca acccggaagc ttctgctaat tcattagctg tcattgtagg gaccttctca
     3361 atattaccga taatgtagtt ggccaacttt ttttctgctg aagaataatc ttccatcata
     3421 ctttcaatac gactaattac gctcataagt tcctccaccc ttttaatgat agtctatgta
     3481 agtataccat atcagaccct tattcacggt cgtatactgg ataaaaaaca aaaaaacaat
     3541 ccgcccaaca gaatatctta tcccatcaat cccacagatt ctattgagca tctgtgtctt
     3601 tgaggaatac ctgttatcta ttgttcggat tgctttctat ctactacttt tttacaggaa
     3661 atttacctat tagttttctc tgctgtaatt tttgacttct gcttccatag catcaataaa
     3721 gatattcaaa tcttctacag ctgtttcttt acttccataa cgacgtacgt tcactgttgc
     3781 atcttccaat tccttgtctc caacaactaa ttggtaaggg atcttttgtg tttgagaggc
     3841 acgtatctta tagcccattt tttcattacg atcatcaact tctacacgca accccttcat
     3901 ttgcaaacgt tctttgattt cataagcata atcgccatgt gcatcaacag aaacagggat
     3961 gattgttgct tgtataggtg ccaaccatgt tggaaaagcg cctttgtata cttctgttaa
     4021 ataagcgaca aaacgttcca ttgtagaaac aattccgcgg tggataacta ctggacgatg
     4081 cgtattttcc ccgtcttctc caacatatgt caaatcaaag cgctctggca ataagaagtc
     4141 taattgtata gttgataatg tttcttctgt accaagagct gttttgactt gaacatctag
     4201 ttttggtccg taaaaggctg cctcgccttc tgcttcaaag taatctagtt ctaattcatc
     4261 catagcggct ttcagcattg cttgggcttt ttcccacatt gcgtcatcat caaaatattt
     4321 atctgtattg tttggatcgc gatagctcaa gcggaaacga taatctgtaa tattgaagtc
     4381 agcatatact gccaccatca attcaagcgt acgtttaaat tcatctttga tttgatcagg
     4441 acgaacaaat gtatgaccat cattcaacgt catttctcgt acacgttgta aacctgacaa
     4501 agcacctgat ttttcatagc gatgcatcat tcctaattca gcgatacgaa ttggtaattc
     4561 acgataagaa tggatcgtgt ttttgtagac catcatgtga tgagggcagt tcattgggcg
     4621 aagaacaagc atttcgccat ctcccatatc cattggcggg aacatgtctt cttgataatg
     4681 atcccagtgt ccagatgttt tgtacaactc aacatctccc attattggtg tatatacatg
     4741 ttggtaacct agactaactt ctttgtctac gatataacgt tcgatcgtgc gtcggattgt
     4801 tgcccctttt ggaagccaga atggcaaacc agaacctact tctggggaaa ccatgaacaa
     4861 gtcaagttct tttcctagtt tacgatggtc tctttctttt gcttcttcac ggagacggat
     4921 atattctttc aaagctttct tgtcaaaaaa ggctgtcccg tatacacgtt gcatcatttg
     4981 attgtttgag tttccacgcc aataagcacc tgccactgat aataatttaa atacttgtat
     5041 acggccagtg gatggaacat gtggtccacg acacaaatcg atgaattcgc cttgttgata
     5101 agcagtaatc acttcttctt ctggcaattc actaatcagt tctactttgt acggatcact
     5161 tgcaaaaatt tctaatgctt cctgttttga aagaacacgg cgttcgatcg gcagattttc
     5221 tttgacgatt ttcatcattt ctgcttcgat tgccggcaaa tcttctgcag ttacttggtt
     5281 ttgtccatta tctgtatcgt agtaaaaccc tgaatcgata gctggtccaa cgccaaagtg
     5341 gatatttgga tagaggcgac gcatggcttg ggccatcaaa tgagctgctg aatgacggac
     5401 taagccaagt gcatcttcat ggtctggtgt gatgatctca atacttgcat cttcctcgat
     5461 tggacgagtc aaatcaacga gttttccgtt cactttacca gctaaagctt tttttgctaa
     5521 gctttttgaa atgctttcag caattgccaa tgttgttgtt ccagactcaa attctttgac
     5581 tgcaccatct ggaaaagtaa tttttatcat aatagtttcc tccttgaaat gttttgattg
     5641 atcagatcat caagctgaaa aacaaaaaaa tcctagcatc tcttgaaaag agatactagg
     5701 acgaaaagtt cgtggttcca cctaatgttc aaacgcaaat gcgttcctca agcgttgtaa
     5761 cggtccgacc gcccttgctt caacaagggt ttcgaaagtg gtcatccttt ggttttctcc
     5821 tggaaaagtt tcagcctagc ttttcctctc ttatcgaaga ctctcaaatt cagttggctt
     5881 tctcatcaat caattatgtg tttatttaac cactaataaa tagaaaaatc aagtgggaaa
     5941 ctaaacttat tttttccatt ccccatttac accgtatgtt ccttttttca tctcattgat
     6001 gatgacatgg atgtgttctt ttggtgcctg cgtgttttta tgaacagctt ccgtgatatc
     6061 tttcatcata ttttctaatt gttccggact gcgaccttct actaattcga catgtacgaa
     6121 tggcataatg ctctctcctc tttctgatcg ttcttttttc aactcgattc tatcatacta
     6181 tgtttgtctt gggtactgaa tcaggaaaaa tggctggatc tttttttgct ctcaaggcac
     6241 tcaaaataga gaccgtatcc acgacttctt gcaaaacagc tccgaataat gcggggatta
     6301 gtcccgtact tgcaattaac atcaaaaaaa tacaaataaa gatgccaatc agtacagact
     6361 gtttcgctat tctcatcgta tctcttgaaa tcactactgc ttttgctaca cgcaagagat
     6421 catctttcaa gatgaccgca tctgcacttt cactagcagc tgtagaacca tgagccccca
     6481 ttgcaatacc gatatctgca gcagcaagtg ctggtgcgtc attcacgccg tctcctacca
     6541 tcgcaacagg tctttgatct ttagggatac tttctaaaat ggtcagttta tcttgaggca
     6601 agctacgggc atgtacagaa tctaattgga ctttttctgc aatcgtttga gcaatggcag
     6661 atccatcccc ggtgatcatc atcaaatttt tcacatcaag ttttcttagc tccttgattg
     6721 tctttgctgc ttctgggcga atctgatccg aaaataggat cgttcctgca aaagatcgat
     6781 caattgttac atagattgct gtattggacg tttctatttc ttcagctgaa acaaaagagg
     6841 cacgtccaat ctttacttca tgattattga ctactgcttg gattccttga ccggtcactt
     6901 ctttcagttg actaacagga tacagttggc tgtttttcac gttgttcact aacgaacgag
     6961 ctaagatatg gctagattcc tgttctgcac tagctgcata cagaagtagc tcttcttttt
     7021 ttattccatt gactggctga atcgcctcta cttccaatat ccctttggta agcgttcctg
     7081 ttttatcaaa agcaatcgtt tttgtacgtg cgagcttttc aatcgttgtt ccagttttta
     7141 caacgatccc gtttttactt gaacggctca ttcctgcaac taaagcaatg ggcgcagcta
     7201 gaatcagtgg acaaggtgat gccactacca gtacctcagc aaatcgtaaa gggtcttttg
     7261 agaaccacca agaaatacca ccgatcaaat aagcaatcaa cgtgaaaggg actgcataac
     7321 gatcagctaa acggacaaat cgggcaggct tttcttttga ttcttcgact agtttaataa
     7381 tcatttgata ctggctgtct gcggcacgtt tttcagcgat catttggatc gatccgtctc
     7441 catttaaaga ccctgacata atctgatctc caggtttctt ggtttccggg cgggactcac
     7501 ctgtcaaaga agattcatcg accgaagatt ctcctttggt cactcgacca tctgcgggta
     7561 caagttcgcc aggcttgact agtaactggt catcaatatt tatttcatcg attactatat
     7621 cctgcaattt tccttctttg aatcgatggg caattcttgg ggtgttatct aacaaagact
     7681 gcagttcttt acccgctcgt ttattcgcgt aatcctctag actatctccg cccgtaagca
     7741 tgatcaatat catcaaacta gcccagtatt ctttgacggc taatgttgca aggattgcgg
     7801 taatcgctaa tacatctacg ccatatcttc ctgaacgtaa cgtcttgatc atttccaaaa
     7861 acatcaagaa ggcgagaatc cctccagtaa atgcgacaat cgcaaatgca agggccggct
     7921 ggtgaaaaac aaattcactt aataaagcta tcccaccaat aagcaaagta agtaaaaatt
     7981 ttttgaaatg tgtcattgtt ttttctcctc ttttatgtga cttagagagg ttagtaataa
     8041 atttccagct gcctctttca tcttaagcat agtccttgac tagcactttt taaaacaata
     8101 ggccttaatt gaaaacaact atcactatca gcgtttccaa aattcatcag ttctacaaac
     8161 aaaaatattc agcaaggatt actttttcga ttcatcagtt aaaaagtacc ttttttcgaa
     8221 aaactatgct atacttttaa ttcgttattt acggcggaag gagcaaacat gcgatgaatg
     8281 agaaacaact agaacaaacg cacgtggatg aaacgatcga actaatccag atcgaacaaa
     8341 atatgctgga tacaaaacaa caagaattga ctcaggaaat gaatcattcg acaaaagaca
     8401 cagctaatca caagattcga gctggttcca atgaagcttt ttatgaatct gctgttgaat
     8461 atcgccaaca tgagcaggaa ttgcttttga aataccatac tcttgaagca cagcaaaaac
     8521 gcctgcaaac gttgtctgtc atgaaagata gtccttattt tgcccgtatc gactttaaag
     8581 aagaccgtga aacagaaaca ttatatctag gtatcgcttc tttaagagat ctggcagaag
     8641 aaacaatcgt tattgactgg cgcgcaccaa ttgccaatct ttattatgaa ggtgagattg
     8701 gtcccgcatt ttatgaaact gaaacagata gaatagatgt cgatctgcag ttgaaacgac
     8761 aatttaaaat ccaagaagga cgtattttat ctatggtaga tacgtcggaa gtaatcaatg
     8821 atgacttttt gctagagata ttggatgaat catcaagcaa ccatatgaaa aacatcgtct
     8881 ctacgataca aaaagcacaa aatgcaatca ttcgggacac aacaagtaaa gtaatgctga
     8941 ttgaaggaat cgccggaagt ggaaagacat ccgctttgct tcaacgtatt gcttttctgt
     9001 tgtatcataa tcgcaagtgg ctcgaggctg aaaacgttct tctattttca cccaaccatt
     9061 tgttttctga ttacatctcg actgtattgc cttcattagg tgaaagcggc gtgcctacac
     9121 agacattcaa aaattatttg gtgcagctgc tgccagagtt ttctttacta gatgaacagc
     9181 aacaagaaag tggttttttg ctaggggaaa aagatccgat acaagtgatg aaaagcggat
     9241 tgaccttagt cgatcagatt gatcgctata tccaaggcat cacaaactac ggtcccttgt
     9301 tccgtgatat gaaaataaat ggccgtacga tcctatcaaa ggaatcgatt cgccaatggt
     9361 ataaagaaac aaatgaacaa ttacctttac atcatagact ttctctatta caaacgaagc
     9421 tcctgaaaaa attaggcggt ctgcaaaaag acgaaacacg tcagcaatgg gtcaaagatc
     9481 tggcggaaga acagctccaa gaactatacg caacagatcc aaatcttgaa tatacagaac
     9541 aaaaggagcg ttcattacgt aaaaaactgg ccaaccaaat cgtacgtaaa agattccgtc
     9601 gaatccatcg agggatcatg aattatcaat ttgtcaatat aacgaaacaa tatctccact
     9661 ttctacaaac tgtgccactt tctcttctaa cacgacataa aatcagcgag accaggtggc
     9721 aggaacattt gacaactgtt cgtacacact tgcgtcaaag agaattgcgt caagaggatg
     9781 ctgtactatt ctttttactg atgcgccgaa catatccagt gacggttaca cacaaagctc
     9841 gctatatctt tattgatgaa atgcaggatt tcgcaccatc acaagtggca ttgctgcaag
     9901 ccttgttccc aaaagcaaat ttgactttct gtggagatct aaatcaaaat gtctttggaa
     9961 atgaaacgat cgttggatca ttggaagacc ttttcgtgga ccgtgaagtc actcgtttcc
    10021 aattaacgac aagctatcgc tccacaaaag aaatcactga ttttgctaat cattttattg
    10081 tgaaagaaca tcaagtagaa acaacagcaa gaaaaggaga ccttcctttg ctcatccaag
    10141 gaacaacaga tgatatcaaa ttgaattggt tagcagatac cattcgaacg actcagggaa
    10201 aagcccggta ttggcgtaca gcaatcatag ctaaaacaca agaagaatgc gaagctcttt
    10261 atgctcaact gccagacgag ttgaaacaac aagtccagct gattttaaat gagagtgact
    10321 ttatgaagcg ttccattatt ttacttccag cctttttagc aaaaggtctg gagtttgacc
    10381 gagttttctt gtgggatgta gatgcagaac atttttcttc tcagcaagac aaattgatcc
    10441 tttacaccat gtgtacacgt gcgatgcatg aattgaccct ttttacctcc aataatgaaa
    10501 gtcctttgtt aaacaatatg gatatttcat cttatgagaa aaaagtactg taagtaaaaa
    10561 gacaactgct atcattaatg ttagcagttg tctttttatt tacaataata attgcagaaa
    10621 aagtaattca atcatttaag gtcatttatt ctatatataa atccaaatct gtttcaggat
    10681 attttaattt gacaagcgaa ataatcattt tcagttcttc catgttttct ggatattctg
    10741 ggtcaaatac cttgctatga cggccaatcc cgccattgtc gatcaatcgg taaattcgat
    10801 tccattcaat gaattggtca attgattgga aattgctgat tggatagccg ccacaatcca
    10861 aaatctctga tacacgaaat gctaggtatg cactatcgat cactttattt tttttcgtgt
    10921 ctatcttctc atttctttcg ataagaattt tttcaggacg atgtttcaag ctccactcta
    10981 atagtagaaa gaaagcctcc atccgatact cttcttcaaa taaccgaaag gctgaatcag
    11041 aacgacgaaa aagcagcgga atcagataat tttgtctata aatcagcatt ggtgcgcggt
    11101 ccaccataca ctctaaataa cggttcagat accctgtacc gacatagttt cctaatcgat
    11161 agccttcaac gccgaatctt gcgtattttt gcgctaattt tgaacaatca gttacgttca
    11221 cagctaactc ttctactttg cggtaaccgc ttaagatacg ttcaaaaggt tctaaagtga
    11281 tttttgcttc catattcttc cccgtccatc ttccacatac ttcatatcat ttcacatgat
    11341 acttatgctc tcatcatacc acaggttgaa ttagaaaaga aaataaatca cacaaaaaag
    11401 caagacaatc tgttagtgag attgtcttgc ttttcgaaaa agactgaata cttttcgtta
    11461 aaactggttg cttagtcttc taatgccaca ttatggaaca catgctgtac atcttctaga
    11521 tcttccagtg catcaatcat tttttcaaat gtttcttgat cttctggaga tagtgttacc
    11581 tcgttttgag ggatcatttg gatctcagcg attgaaaaat cagtgattcc tttttctttt
    11641 aatgcttctt gtatactatg gaaatccgct acttcaccgt aaacgatgat ttgtccttct
    11701 tcttccatta catcgcgaac atcgatgtct ttttccatta ggtattcaag gatctcgtca
    11761 gcgtcttctc ctgcaaaagc aaagattgct gtattgtcaa acatgtaaga aaccgctccg
    11821 gaaacaccca tgttcccgcc atttttccca aatgctgccc gtacgtctgc tgctgttcga
    11881 ttcacattgt tcgtcaatgt atcaacaatc accattgagc cgcttggtcc aaatccttca
    11941 tagcgcaatt cagagtatgt ttcatctcct gcaccttttg ctttttcgat tgctcggtca
    12001 ataatatgtt ttggtacatt ataagtttta gcgcgatcaa tgacgaaacg tagtttttgg
    12061 tttgcatgcg gatcaggatc acctgatttt gctgccgcgt agatttcaat cccaaatttt
    12121 gcataaatcc ggctattatt agcatcttta gcagttttct ttgctacgat atttgcccat
    12181 ttacgtccca tattttcact tcgctttctg atttgatagt ttcataacgc aaaaacgtcc
    12241 tgttccatta tacttgtgaa tcacgattct gtgaagcttt ttttctttct tccttcattc
    12301 ttctcatttt tagtcaatta tctatactcg gattgggatt tgcccgaaat tctgttccgg
    12361 aatattgcct cccaattgat agatttgatc acttctaact aacagttccg cttcttgtgc
    12421 ctcttttttt ccgattcggg aataaataga aatctctttt ttttctttta agaatcttct
    12481 tccagcatcg gtaaatccaa gtagtcgaag ataattttta ttttgttgga caggaatctc
    12541 ttgctttttg atattcagca agacataaca agataatcgt tggatccgtg tccatgtata
    12601 tctttttgtt tttacctttg acacaaaatc ctcaaaacta tcagcagttg cagctgctgt
    12661 tttcatgcgc gattctattc cttcggtcat ctgatagata tctgtcagtt ccagcaaagt
    12721 agaagataac aaccgataac gcaaatacgg ccaataagat tcccaagtct gcagcgaaca
    12781 agttgaaaga tcttcttctg tctgaggcgg cacaagtcca gctattcttt ttccttcttc
    12841 taacgcatgg cgaatggctg ttgcactggc tatcctctga tctgtgatga ttttttcatg
    12901 atagcctgca cttttacgtc ggatcggatg taattccata gggtggacat acttggcatt
    12961 ttcctttgca taacttaatc ccaagatatg atttggcgaa gaaaaatcaa gcgtaatatc
    13021 gggatacaga ctgcgaaaaa cctcagtcat tttttgcgga taactatacg tttgatcaga
    13081 cagttcttga aatgcctctt ctatcctatc ttgattctct tggacaaagc gtccgaaact
    13141 gctgtaatca aacgaagtct cttcttctgt tccaaaacac aaactgtcac agtccagtgt
    13201 ttgcaagatt tttactgcac ctttagcaaa atagtcagct gactgaaccg cccattcgac
    13261 gggaagctcc actactaaat ccacaccatt ttgtaaagct tcattcgcgc gatgccattt
    13321 atcgatgata gccggttcgc ctctttgcaa aaaattcccg ctcattacag caatgacgac
    13381 ctctgctcct gttatttttc gtgccatttc cacatgatag cgatgtccat tgtgaaaagg
    13441 attgtactct acgataatcc cgcagctttt catgcgcatc ctccttcata gttatcgata
    13501 aaaaagaaat agagacaagt aaaaaaaggc aaaaatcgga aaaaataccc gaactatata
    13561 aactattctc gcaaacacaa tacttctgcg acgaaaagct gcactgcaag agcaatgcct
    13621 atatcgttcg gatatataat ttatgttgaa gatttattct tttcttgtta actcgagcag
    13681 gcaaaaccgc tagtctttgt atgctacaaa gaaccagcgc tttgatttct ctgatgctgc
    13741 ttcgtctgta aaatcagaaa atgcttcgac tttcgaaaag ccgctgtttt ccagcattct
    13801 taaataactt tccaatggat aggtacgctc ttgatgtagt tcatcttcac gaataaatcg
    13861 accagaccct tcttgttctt caacgaaaaa ggtaagaaaa tgttcgatgc tgtgtttctc
    13921 gcttcctgga taactatccc acaaaaaagc aaaattatcc ccttgataat gatagctata
    13981 ttctggaaaa acctcatcta cttgataggt ggagtgtacg tcaaataaaa atcggccatt
    14041 ttcttctaac gcttgataga cttcatcaaa cacttgctgt acctcttgtc tatttttcat
    14101 atagcacagc gaatcagaaa aacaggtaat tgcctgatat tgtccgattt ctgataaatc
    14161 gagcatattg ccttccacaa attggatcga tgcgtcttct tcatatgctc gctggctggc
    14221 aatcatcaac atctcttctg aaaggtctaa ccctgtcact ttatatccat cttttgcaaa
    14281 ggcaaccgct aatgctccag taccgcatgc cagttccaaa atattttttg tgtcttcttg
    14341 taaatgacgt ttcgaaaaag ctaaccattg ctgataaagt gtttcgtcca tcacttcatc
    14401 gtaaatgaaa gcaaatgttt cataagccat atgttaatcc aaccattcac taagatcgac
    14461 tagcggagca tctgaccata gtttttccag attatagaaa tttcgttctg gcgcatggaa
    14521 aacatgtacg atcacatctc caagatcgat caatacccat tttccgcctt cttttccttc
    14581 aatacgtttt acttcatatt tgttttcttc ttctttatca atgatttctt ctgtaattgc
    14641 gttgatttga cgttcgctat ttgctgaaca gatcatgaaa taatctgcca acaaggaaac
    14701 atttcgaacg tctaaagcga taatgtcttc tgcccgtttt gaatctgctg cttcaatcgc
    14761 aatttttaat tgttctttac tatctatgat gatttcctcc taatatgagc agcttatttt
    14821 tttgctaccc agtaattata tgtttcgatt gtttttggat aaatctgttg ttcttgcgca
    14881 agcaaatggg ccaatgtatg ttttgtttca aatgctacag cttcgtctag atcagcccat
    14941 gcaatggctc gtgcatcttg taccccagga aaatctcgac ctggttcaat atagtctgct
    15001 acataaatga ttttgtcaag gagactcatc ttagcagccc cagtcgtatg cacccggatt
    15061 gcatgaagaa tctctgcatc ggtgattcct aattcccgct ctacaaacga agcgccaaca
    15121 agaccatgcc agattgcatt attataattc aataactccg gatcaaaacc gtctcttacg
    15181 attaccattt tgaactcctc atctggtcgt tcttttgcat aatcatgtgt caatgcagca
    15241 atactagctt tttcaggaga agcaccgtat tttttggcaa gagccacggc tgtttcttct
    15301 actccaagca catgcttgaa gcgttgttcg ctcattctca tctgtatttt ttgcatcagg
    15361 ttttctctag aataagcagt atattcatta ctatagtcca tattcataca acccttcatt
    15421 ttgaatataa tcaattactt tatctggaac aaggtagcgg atagaacagc cttctttgat
    15481 cttttgacgt attttcgtgg aactgatgtc gatctcaggt acatctaccc aaattacagg
    15541 atatggagta tccgttgtgt atcctggtcg acgtataccc acaaagttga ccatagaagt
    15601 cagctcatcg attttatacc attttggcaa atattctacc atatccccac cgataataaa
    15661 gtaataatct gtatccggat tgttttgcgt caattccttc atagtatcat aggtgtaact
    15721 tttcccacca cgagctaatt caatcgtttc aatctgcaaa aaaggattgt cttccaccgc
    15781 taattccaac atgttgagtc gatgttttgc atcaatcgtc tgtttctcat caacatgagg
    15841 tggctgataa gaaggcatca aaaatacgcg gtcaagacct aaattccggc cagcttgttc
    15901 agccatcact aaatgtgcaa ggtgaacggg attgaatgtt ccgcccaaga tccctacttg
    15961 ctttttcttc tctaagaatt gaggcatttc ttcaggaaat aattgtgaac gaacaaatgt
    16021 cttagcttgc gtattcatca tggtcactcc ttatattgct tttacttctt ttgatagcct
    16081 ttgatacttt tcttttgtgg aaggtttgta taatacaata acacgtccaa tgatttggac
    16141 gatttcgcaa tctatttctt tttctaatac ttgagctgct tcttctgcaa tctcatctgt
    16201 attttgaagt aacgatactt tgatcagttc tcttttttct aaagcttctt caatttgaac
    16261 gatcattgct gaattgatcc caccttttcc gatttgaaaa atcggctgaa gatgatgcgc
    16321 tttgctacgt aaaaaacgct tttgttttcc tcttaaactc ataattttcc ttcttcctta
    16381 aatcaatgct ttgcgtttca atacatctac accctttggc gcccagcccg caattactac
    16441 tggcgctgtt acagtgatcc agcctagacc ggcaaaaacg atgtctgttt tttctttcac
    16501 agaaaactca aaacgaacaa gctccggaaa tgcctcgact tcatcaggac gaggaggctg
    16561 taataatcca cctacatgtt tttgataaaa ttcgtctgct ttttccagtt tcgttcgatg
    16621 aatatttaca tcatttgaca cataagcaat aaatgaagca cgattgccgc tgataaaatc
    16681 aaatcttgca agtccgccca agaaaagtgt ttgcccttca ttcagctgat aaactttagg
    16741 tttgatttct tttaacggtg caatgatccg caaatctttt ttacctaagt agtgcgccat
    16801 ttgatggcga tggataattc ccggtgtatc aatcaaaaaa tgcccgtcat ctaaaggaat
    16861 ctcgatttta tccaaggtcg ttcctggaaa ctgcgaagtt gtgattacat cttgtactcc
    16921 cgctgtattt ttgatgattt gattgataag cgtcgatttt ccgacatttg ttactccgac
    16981 gatatagaca tcccgtccat cgcggtattc ttcgattttt tctaatagtt ccgtcatctc
    17041 atgagatttc ttagcactag ttaataatac atctactgga cgaattcctg cttcgtaagc
    17101 acgttccttc atccattgtg ttaatttagt ttttttcaat gatttaggta atatatccac
    17161 tttgttccca actaaaagta ctggattgtc accgacaaaa cggtgtagtc ctgggattag
    17221 tgaaccattg aagtcaaaaa tatccaccac attgacgatc aatgcatctt tttgccctaa
    17281 ttcattcagt aagcgcaaaa agtcgtcatc cgtcaatgag acatcttggg tctcattata
    17341 atggcgtaat ctgaaacaac gctgacaata gacttccccg gaagctaaac ctttttccaa
    17401 agccgtttgc ggcgtgtatc ctaattcacc gggtttgttt gtttgtataa cggcaccaca
    17461 gccgatacat tgtaattctt ctgtcattcg attccacctc gccagcccat atcagggtgt
    17521 tttgctaata gataggccat aattcttttt tcccaaaaac gattaaattg tgtcttccaa
    17581 ctatctgttg cgataatggg ttttacaagg atgctacgta tccctgcacg attggctcca
    17641 cgtatatcag tcataatctg atcgcctacc attactatct catcatctgc taaatccaat
    17701 tgtttctttg ctatgttgat ccctcttgct aaaggtttca ttgctcgtgc aacatacaaa
    17761 agatcaaatt tttcaatcgc tcgcgcgaca cgttttgaat tattgtttga aacaacaaca
    17821 acagggattc ctgcattttt catttctaaa atccaagtaa gcaactcttc tgttccatct
    17881 ggattattcc atgcaatcaa cgtattgtct aaatcggtca atactgcttt gatgcctaga
    17941 ttctttaatt gggcaggtgt aatctgatag atggcttcta ccatccatgt tggtttgtaa
    18001 tttgaaaaca tgctgtctcc tcatctgcaa aaaatgaagg agcacataaa gtactcctca
    18061 accccgtatt atactgcata tcagcaaaaa aaactacctc tatactcgtt cgtctttcag
    18121 aaaaagcatt tctttacaaa atgaccaaaa ataaagattg atcacagaaa aatttagaaa
    18181 caaaaaacaa ctaatattgg cggaacctct tgtatcgttt ttccagtaat ttatgctgat
    18241 ctaatcgaac aagttccgtc aatctttcaa tcaacgcttt tttcataatg tgtgcgactt
    18301 tatatcgtgg caactgctcg ccgttgaacg tttcaggaat aactcgatca acgattccta
    18361 actcgcgtag ttctggcgca gtaattttca tcaactcagc tgcttctgcg gcgcgactac
    18421 cgtctttcca caaaatcgac gcaaatcctt ctggagaaag cacagcataa atcgtttctt
    18481 ccagcatcca gacttcatcc gctaaaccta gagcaagggc accgccactt ccaccttcac
    18541 caatgatgat ggcgatgatc ggtaccttta aatcagacat ttcgagcaga tttctggcaa
    18601 tagcctctcc ttcgccgcgc tcttctgctc ctacaccgca ataagcacct gctgtattga
    18661 tcaaggtaat caccggtcta ttgaactttt ccgcttgttt catcagtcgt aaagcttttc
    18721 gatagccttc aggatgggga gaaccaaaat ttctgcggat attttcctgc agatcccgtc
    18781 ctttttgaat accgatcact gttaccggtt tgccatctaa tcgagcgatt ccaccaacaa
    18841 tcgcttcgtc atctgcaaat aaacgatcac catggcattc ctcgaaagaa tcgaagatct
    18901 cttcaaaaaa atccaagctt gtccatcgtt ctttagctct tgctaactga acgacatcat
    18961 gtgctgtctt atccacgatc tatccatcct ttctgtgtat gcaattcaag aagcgtatgg
    19021 attttctgtt tcaattccat gcggggaaca atctgatcga caaaaccatg agataataag
    19081 aattcagcct tttggaaatc ttctggaagt tcctgcttga tcgtctgttc gatgacgcgt
    19141 cttccagcaa aaccgatcaa gctttggggt tctgctagaa taatatcgcc ttccattgca
    19201 aaactggcag ttactcctcc ggtcgtcggg tccgttaaaa cagttagata aaaaagacct
    19261 tcattgctat gccgtttgac agcagcagag atttttgcca tctgcattag agaaaagatt
    19321 ccttcttgca tccgtgcgcc acctgaagct gtgaacaaga cgactggaag actttccttt
    19381 gttgctcgtt cgaataatct ggtgatcttt tctccgacaa tcgttcccat acttcccatg
    19441 ataaaattag gatccatcac tccaataatg aagggaatcc cctgaatggt tgctttacca
    19501 gtgaggactg cttcatgaag tcctgtttta tcttgcattt ttttaatttt ttccgtatat
    19561 tcgggaaatg ctagcggatc tttggttaca agatccgtat cccattcttc aaaacttttt
    19621 tcatcaataa tcaaagctag acgctgccat gcaccgattc gaaaagcgta tccgcaatga
    19681 gggcatactt tttcttcacc gatgtctttc gtataaagga tatgtttgca atttggacac
    19741 tttgcccaca tgttatcagg aacagatggc gagttagcag gtttgctctc tctattagga
    19801 ttgatccgaa tatatttctt tttcttaaac agagccatct atgcgcctcc tatctccgtt
    19861 tctggtgtcc aattcggtaa aaattcttct tgtaaaaatg ctgtgctgta atcacctgct
    19921 atgactgcag gatgactaat caaatccatc tgaaattctg cgttcgtgat gattccttct
    19981 gtgataaatt cacttaaggc tcgctgcatc ttcattaatg cttcaaaacg cgtatttcca
    20041 tggacgatta tttttgcaat cattgaatca taatatggcg ggatcgtata tcccgggtaa
    20101 actgcactgt ctactcgcag tcccatccca cctgctggaa gcagtaagtt ttggatcttc
    20161 cctggagaag gagcaaaatg gaaagcagga ttttctgcat taatccggca ttcgattgcg
    20221 tgtccttgga atactacttc ttcttgacac acattcaaag gctcaccggc tgctatctcc
    20281 acctgttttt ttactaaatc gattcctgtg accatttctg ttactggatg ttccacttgg
    20341 attcgagtat tcatttccat gaaataaaaa tcgccttctt catccatcag aaactcgatc
    20401 gttccggcat tttcataatt gactgcttta gctgctcgaa cagcagcgtc tcctagtgct
    20461 tctctttttg tttgtgagat cactacagaa ggagattctt ccagtacttt ttgattgttg
    20521 cgttgtaaag aacaatctcg ctcgcctaaa tgaatgacat gaccaaatcg atcacctaag
    20581 atctgaactt ctatatggcg agcaggataa atgatttttt ccaaatacat atcatcattt
    20641 ccaaatgccg ctgctgcttc ttgctgagct gaagaaaaat gctgtgggag ctcttctttt
    20701 gattgaactt tccgaatgcc tttcccgcca ccacctgcag ctgctttcag catcactgga
    20761 taaccaattc tgtctgctat ttccaatgct tcttctactg tagataatac accagtactt
    20821 cctggaatga ctgggacatt tgcttcttgc atcaattgac gcgcattgat cttatttccc
    20881 attgcatcga tcgttgcact tttaggacca ataaatgtga tattgcactc ttcacacatg
    20941 gaggcaaatc ggctattttc agataaaaaa ccaaatcctg gatgaattgc ttcggctttt
    21001 gtcacgattg cagcgctcag tacttcctga acattcagat atgaatcaac ggcttttgcc
    21061 gggccaatgc agattgcttc atctgctaac tgtgtatgaa gtgcttcggc atctgcttct
    21121 gaatatatcg ctacggtttg gatccctagt tcgcggcatg cacgaatgat acgtacagcg
    21181 atttcaccgc ggttagctat caatacttta gaaaacatat ttcacctatc caatcataaa
    21241 ggttaattcc gcttcggcta cttttttacc gtctacttta gcgattcctt tgcctattcc
    21301 ggctgaagct ttcactttca atatctcaac ttcaaggtat aaggtatctc ccggtgtaac
    21361 tttcttacgg aattttgctt tgtccaatcc gccgaaataa gccgtctttc ccttgaattg
    21421 ttcaagagaa agtaatgcaa ctgctcctgc ttgagccatt gcttcaacaa tcaatacccc
    21481 tggcataaca ggttcttctg gaaaatgtcc ttgaaaaaag ggctcattga tcgttacatt
    21541 tttcttagca acgatccgtt ctccttcaac catttcttcc actcgatcaa tcaaaagcat
    21601 tggataacga tgtggaatga tttcttttat ttcttgtatg ttcattgttt tccctctttc
    21661 ctgctttgct tatttttgct gaatcctaaa gagtggctgt ccatattcga caactgactc
    21721 gttttctaca aggatttctg tgatcactcc gtcggcctca ctagttatct cattcatcag
    21781 tttcatcgct tcaataatgc agataacttc tcctttggca acttgatcac ctactttttt
    21841 gaaagctggt ttgtcgggcg ctggttgcag ataaacgatt cccacgattg gcgaaacaac
    21901 ctcgattcca tcaaatactg ccgattcttt ttctttcatt atttctggag cagttgttgc
    21961 ttgttcaggt atgacattga ctggctccga aactggcaac gtttcgtttg tttgaaccat
    22021 attcatgcct cgacttgttt tatttttatt catatacaat tcaaattgac cttcacgcaa
    22081 atcgaattcc gttagcgagg attgatcaaa ttgactgact aattctctga tttcattgat
    22141 atccaaaatt attcctccca gcgtttcatg cacagaaccg cattatgtcc gccaaaccct
    22201 aatgaattac tcattgcata agtaaaactt gcaggtttgc tttcgttttt gacaactgtc
    22261 aagtcaatct caggatcttg tttttctaca ttgatcgtag gtgggataaa ttgatgttct
    22321 aatgccagca aagttgccaa ggcttcgatc ccgccggctg ctcccaataa atgtccagtc
    22381 atcgatttcg tgctgctgac gtaagtatct ctgaaatttt cacctaatgc gatttggatt
    22441 gcttttgctt ctgcctggtc attggctggt gtacttgttc cgtgagcatt gatatatccg
    22501 acttgttccg gctgtacacc agcttcttcc atcgcaagct tcattgcttt agatgcacca
    22561 gaaccatctg gagtaggagc tgtcatatgg taagcgtcac agtttgcacc gtatccaacg
    22621 atttctccat agattttggc accgcgagct tgtgcatgtt ctaaagattc aagaactaga
    22681 acgcctgctc cttcacccat cacaaaacca ttccgatttt cgtcaaacgg aatcgaacca
    22741 cgattaggat cagttgattc tgagagtgct gttaaagctg caaatccagc aatccccatc
    22801 tcacaaatcg ttgcttctgt tcctccagca agaatgacat cagaatagcc atgtttgatg
    22861 ttacggaatg cttccccaat tgaattcgtt ccgctagcac aagctgttac gatagctgta
    22921 caaatacctt ttgcacctac acgcaaagca atatttccag ctgccatgtt ggcaatcgcc
    22981 attggtacga acattggaga aactcgtttt gcaccttttt catgtagacg aataacttgg
    23041 ttttcgattg ttggaagacc accgatccca gatccgatga tgacgccaaa ccggtcttga
    23101 ttcatttttt ctttatctaa accgctcatc tccattgctt ctaaagcagc gtagattcca
    23161 tagatcgaaa acatatccat tcgtttgcta tcttttttaa taaaatattt atcgtaaggg
    23221 aaatctttga cttcacccgc taaggtaatc cctgtttctg aagcatcaaa tttagtgatc
    23281 ggtgcgatgc cattcgtccc ctcttttaag ctattcaaaa agctgtctgc gtcatttcca
    23341 ataggtgaag tgactccata gccagtaatt accactcgat tcatttgttt gctctccttt
    23401 tcttatccgt gcatgaccaa tccgccatcg acattgatga cttgtccggt gatatatgga
    23461 ttttgtgcta agaaaactgc tgtttgtgca atatcttcta cttgtccgaa acgctgcatc
    23521 ggaatttgct tttccgcttg ttccttgact ttgtctgcta aaacttccgt catatcagtt
    23581 gtgatgaatc ctggcgcaat ggcattacat gtaatgcctc tagttgcagc ttctcgtgcc
    23641 acagattttg ttaatcctac tacgccagct ttactagctg cataatttgc ttgaccaata
    23701 tttccaatca acccagatac actagagaga ttaatgatgg ccccttctcg ctgcttcatc
    23761 atttttttca atacgtgctg cgtcatattg aaggttccaa tcaaattgat atccagacat
    23821 tttttgaaat cttctgcatc cattcgcatc acaagtttgt catttgtgat tccggcattg
    23881 tttactaaca catggatcga tccaagcttt tcttctgctt ctttgatcat ttgtcccgct
    23941 ttctcataat cagagatgtc accggaaact cctacgcatt ttacaccgaa tgcttcgatt
    24001 tcttctatct tttcttttgg aatctctccg cgaccattca aaatgatatt tgctccagct
    24061 tttgcaaaag ctaaggccat cgctttgccg attcctcttg tactacctgt gataaataca
    24121 ttttttcctt ttacatccat aacgttcctc cttatagtat tgctattgtt tcatcaaatg
    24181 tctgcttatt ctcaacacgc agaactggta ttgttttatc gatttttttc ataaatccgc
    24241 ttaatacttt tccggggccg acttcgatga cttgttcgat gccgattgtt tttaacttat
    24301 ggatactctc atagaaacga acaggttgca tgacttgctg ttcaagtagt cccgcgattg
    24361 tttctttctt cattatttcc gttgttgtgt tgcttatgat aggaaaagaa ggttcagaga
    24421 attgaatctg acttaggtcc ttcgccagtt tttttgctgc cggttctaaa atagctgtat
    24481 gaaaaggccc gctgacattt agagggatca ttcttttgaa ccctttttct tttaatagta
    24541 aaacagcttc gtctactgct ttttcttcgc caccaatcac gatctgctga ggtgtattat
    24601 aatttgcagg agatacgatg ccgtacttgc tagcttcatg acagctttcc tcgatcgttt
    24661 caatcggcgc attcatcact gcaaccattt tcccgcttcc agctggggca gcttctgtca
    24721 tataagcgcc tctttttgct actaacgcaa ctgcttcagt gaaggatagc gcattactag
    24781 ctactaatgc cgaatattct cctaaactca gtcctaaagc tgcatctggg atgattccat
    24841 gctccttcaa taagcgataa tacgcgatgc tgactgttag aatagctggc tgtgtatatt
    24901 gcgtctgatc caaacgttca ttttctgtaa agcaaagttc agccatatcg tagccgagaa
    24961 tctcacttgc ttcatcaaat gtctgcttta caacggcttc ttcatataaa tcttttccca
    25021 taccttgata ttgtgcacct tggccactaa ataagaatgc tgttttcatg tttttctcca
    25081 atatatccag aatatatgtt tgattcttcc tattcaaaaa ggccgagaca ttcctatttc
    25141 aggaaatgtc ccagccccca acctaatttt aatgattatg cagtttgttt ttctacgtaa
    25201 gtcactaaat cttgaaccgt tttgattccg tcttctgttt cgattttcac gtcaaattca
    25261 tcttcaattt cattgatgat ttggaataaa tccaagctgt cagcttctaa gtcttcttgg
    25321 atgtcagtag tcaatttgat ttcttcagtt tcttttccta attcttctgc gatgatttct
    25381 tgaatttttt cgaataccat aataatatat tcctccgata ttgtttgctt tttattttta
    25441 taatttaaca gtctacaact ccatgagaat acttccccat gttaaacctc cgccatagcc
    25501 tgtaaatatt actttctgct gttttcctaa ggtgatcttt ccactagaaa ctgcttcatc
    25561 caataagata gggatgcttg cagcagatgt attcccatat ttatccatat tggtcaggaa
    25621 cttttcttgc ggcatcttta cttttcgggc aattttctca ataattctga ggtttgcctg
    25681 atgcaaaagc agataatcaa ctggtttgtc tttcatgact tcttggatat tgtttgcaac
    25741 gtctcgtacg gcaaaatcaa aaatatctct gccagtcatt tgcaaataat acgaaccttc
    25801 tgaatcttct gaatagaacg gatttttatt ttgctggtag ccagcggtca atgagctact
    25861 tctggtaccg tcagctgcta atttttcttt cagcaatttt ttctgtggat tcgcttctaa
    25921 gagaatccca cctgctccat cgccaaacaa cacagcagtc gtacgatcag accaatcgat
    25981 tagcttagat agtgtttcac caccaatcac cagtccctta gttgagccgc agcggatcat
    26041 tttttcagcc atgcttaacg cataaacaaa tcctgagcac gcagcactga tatcaaaagc
    26101 gatagcatga gttgctccca atttcccttg tacttgtgct gctacagatg gtgtcgtgta
    26161 atcgggcgac attgttgcta ctaaaataaa atccaaatct ttagggttta gatttttctg
    26221 ttttatcaat ttttccgcca ctaaaatgca taaatcagaa gtctcctgat ttgttacaca
    26281 tcttcgttca cgaatacccg tccttgacga aatccattca tcagaggtat ccatcatctt
    26341 ggaaagatca ttattagtaa taatcttttc aggaaggacg ctggctgttg cagtaatcat
    26401 tccaaattgt tccattaaat atcctcactt atagtcttgc aagaagtcgt gcaagttttt
    26461 cagtgcatcc agcaaggcat gctgttcctc cgtagccatt ccgtctaaga tgttcttgac
    26521 catttcacga tgaaaatgct gatggacccg aaatagtaac tttccttttt tcgttagtcc
    26581 caactttaca acgcggcggt catcttcact gcggatacgt tcgacatagc ctttttttac
    26641 taacttgtta atagcagtgg tcaaggtccc aacagtaatt gatagttctt tggctacttc
    26701 tgaagttgtt tttttcttgt acatcccgat tgcctcgatc gtgtgcatct ctgtgattga
    26761 cagatctttg aattgagatt ttttcagctc agactcttcg attgtcaaaa tatcattgaa
    26821 gacactaaca agataatcat ttactgtttt taaatctggt tccatgttcc accgtccact
    26881 aattagtttg attatcaaat cttttgatgt tcaaactata tcttaacttt tttcaaaatg
    26941 caaccttaaa agatcatttt tttactatat gagctttttt taagatttta ctcatttaaa
    27001 acaactgata aatcagcatt tataatttct tttattagaa aaatcatcta tctcacatat
    27061 tttgattatc aaagtatttt ctgtgaacag aactatagaa ttccctaaaa cttgtccatt
    27121 atttcaaaat ttgttatagt ctttttattc aaagcgtaaa aggagatcct atgttaaaag
    27181 aaaaaatgaa aactctcccc atgctgccgg gtgtctatct tatgaaagac cgagatggta
    27241 aggtcatcta tgttggtaaa gcaaaacggc tcaaaaatag agtaagcagc tattttcatc
    27301 agaataaaca gcattcaaaa aaagtattac gtatgattca tcatataaca gattttgatt
    27361 tcgtcgtagt agacacggaa ttagatgctt tattgctcga gtgtcaattg atccaacatt
    27421 accgtccttt ttataaccga caaatgaatt atttcagtaa ttataattat gtacatatta
    27481 ccaataaggg atttgtatta acagatactc ctacggctcg aacatacggg ccttttcgtc
    27541 tctataaaaa aatgccgtct attttgcgta taatggaaga aacttatcaa atgccttggt
    27601 tgtctgaaat tagtttgtta gctttgcgag tccagcttcc tgacctacag gaaatgactt
    27661 ttgaacagaa aaaaaaggaa ctccaagggc tgtttcaagg ccgaaataaa aagctactga
    27721 cttacttgaa aaaaagacaa caacatttca tctatcagtt gaatttcgaa aaagccggta
    27781 tgcttcaaaa agatatcgaa ttagttactt attttattag acggatccag gaacaaaagc
    27841 agtttttaag aacaccatcg ctcaccttca gcatgccatt agcagcagat gagagtcaga
    27901 aaaagcacta cctcgtttgt tatggtcaac ttgcagaaac aatgattacc tctggtgacg
    27961 tttctcctga cttctattat gaaaagaaag aagctcattt atctttgaaa cgtcaattgt
    28021 caaaagaaga aatcgatcct gtacaaatct tgatcagtta ccgcaaaaaa ttagaaaaag
    28081 aacagtttga aattgaactg ttaaacaaaa aagaagctga aaagcaacta aattagttgc
    28141 ttctcggctt cttttaggca tcttcaactt caaatgtcaa ttatcctaaa atttattgga
    28201 ttaagtcata gatttccatc gctacgatat cgatattatc aaattcgtat tgctgatgag
    28261 tatctgtttc agttaattca aatgtatcgg tcgctttatc ataagttact gtacacttct
    28321 ctacgccttc tttttcaaaa cggcgaactt gcagttcatt atctgctgac tcgaccatag
    28381 cctccagtcg ttttatgatt gcgactaatt gtgaatgtgt catcggaggt cctccttttc
    28441 ttatttctgt tatattgtat caaaagcata gcctacttgc attgattttc tctaaaaaaa
    28501 ctcgtttttt atgatttttt ttctggtatt tttccttcag gccaccaaag tttgatcaaa
    28561 gcttgggttc cgtcattctc aaaaccttta tctgccaact cttcatatag tttcaatgct
    28621 tgggtagttg ctggcaatac aagatccatt ttttctgctt cttctaatgc aatctttaaa
    28681 tctttgataa aatgtttgac aaaaaaacca ggtgtgtaat cttcttttaa aatacgagga
    28741 gaataattgc ttaaagacca gttagctgca cttccacctg aaagcgtctc aatcactttc
    28801 tttaaatcaa gtccgttttt ttgtgcatag acaagcattt ccgtcattcc tgtcatcgta
    28861 cctgcaatca tgatctgatt agccattttt gtatgctgac ctttcccagc agaaccatgc
    28921 aatgtaaatg tcgtaccaaa ttctttgaat aaaggcagta cttgatcata aacggcttct
    28981 tctccaccga ccatgatcgt cagtgtacca ttctttgctc ctaagtcacc accggatacc
    29041 ggtgcatcta acgcatctgc gcctttttct tttgctgttt ttgcaatttt ttctgccagg
    29101 cttggtgtac ttgtagtcaa atcaactaaa atatgtcctg agatatctgc agaaaaaatc
    29161 cccgactgtc catagtagat ttcttctaca tctttggggt aacctaccat tgtaaagaga
    29221 atattacttt gttctgctac cgcttttggt gtatctgccc agatagctcc ttctgttact
    29281 aaaggatcag cttttgcttt tgtccgatta tagacagcta cttcgtgtcc tgcttctagt
    29341 agatggcgtg cgacagcact tcccatgacg cctgttccga taaaaccaag tttcattttt
    29401 tcgacctcct aaaaatcaaa aatccttgct tctatgctag tatacgctag aaacaaggat
    29461 tttttcataa aaaatgtttg aattttatct tttaaatgaa tttaatttta ttttgattcg
    29521 ttcaatcgag tcttcttctt cttttcttcg catctcttct tcatgataaa caaccatgtc
    29581 ttgataaaat tcgatcatag cttcgccgaa atgtgtagat gaaatatcat aaagtttagc
    29641 ttctaaaact tttggatctt taggaacttc actggaaata taatcaataa gtgctggtgc
    29701 aaagtcttcg tcggtttcaa aagttttccc taatgaagga tgatcgaaca atcggttcaa
    29761 ataagcattt ccttccacga cacaaggtgt tccggctgcc atcgcttctg tataagtaag
    29821 cccttgtgtt tctgaagtag aagcactgac aaaataatct gccgctttat aataaagtgc
    29881 tacatcattg ttgtccactt ctcctgtgaa ctgcacaaat tcttctaact gcagttctga
    29941 gatcaattcc tctaactctt ctacataagg tccttttcct acgataacta atctggcgtt
    30001 aggataagcg gagatgacct gaggaaaccc attaatcaag gcttggatat ttttctcata
    30061 agaaatcctg cttaaagaaa gaatcatcaa atggttttcc tgaagaccta gtgactccct
    30121 taatttgctg atatcctgct gagtgatatc agaacgtttg aatttttcaa tgtcgatacc
    30181 agtaggaatc acgcgaagcg gtgctattac accatagctg cgtaatgtat caataacacg
    30241 ttcacttgga caaactacac ctgtcgtatg atttgcaaat aatctagaca agtattttac
    30301 atgggattgc ctgatcactt ttcctttacc gatataatga aggtagtctt catacatcgt
    30361 atggtaagta tggatcagag gtattttcaa cttcttagct accatcttcc caagtatccc
    30421 tgctccgaat tctgtatggg tatgaataag atctagttct agttctttgg caaccaaata
    30481 agcataccac atgcctctaa caacaactct tcgatccttg aaagaaataa atggtacact
    30541 tggcattcga atcacatctt tttccaactt atccgcatta ggatcggtcg ttgtaaaaat
    30601 atagacttca tgcccatgtc tttctaattc ttgttttaat gttttgatcg atgtagcaac
    30661 tccgctgact tgcggaaagt aggtatcggt aaaaaagccg attttcatgt ttttcgcctc
    30721 caaactagtc cttgacctga tttacttttt ttaatttgct agagactttt tcattcagta
    30781 cactttcgta tacattttta agttcttcac cgattttatt gatacttcgc tcttttgctg
    30841 tttgataacc ggcttgtgtc gtactcggta gttcttgatt ggtaatctgt tggatcagtc
    30901 ggataaattc ttcgtttgtc ttccccatat agcagttctt gctatcttct aaccagccat
    30961 tatacactgg aatatctcga acaagtacgt tttgtttgct tgctaaagct tctaaaacaa
    31021 cgatcccttc tgtctcttca taagaaggga aaaagaatag atctgcacct gaatacgcac
    31081 cttctatgat ctctccacga atgtaaccag gaaacaggac attttcagga tgatcttctt
    31141 ttacaatttt ccggatgttc cgaggaatcg agtacatggg aacatgacca aaccaaataa
    31201 atgtgtattc gggcatcttc ttagcaattt ctacgaaatc aatgattcct ttacgttcga
    31261 aaaacaaacc gacacaaatg attacttttt gatccggaga cagtttgaaa tattctctga
    31321 acttttgttc tttcattgga tcagggtgat atttctctaa gtcgatccca ttggaaatag
    31381 cttgaatcgg cacacggata ccatagcttt ccaacaactg ttttgaataa ggtgttggcg
    31441 tgatcagata gtccgctttt tggtacaatc ctaccagata tttttttacg ataggagaaa
    31501 gctggttcga tctgataaag gaatttcgga aatcttcctc tgtagaatgc gcatgataga
    31561 caactttttt tccttctctt ctggctttat tgaccatatt atgactattg attccgtatg
    31621 tattgatgtg caggatgtca taatcttctt tttcatctag tgtataagag ataccgactt
    31681 ccgtcagagc acgtttttga tgatcaagcg cacgcccgat tccagatttt gcaagcatct
    31741 tttcactttc aaaatataat aataccttca ccttacgtcc cccttcactt atatagtata
    31801 acatagccta caaactgtca ctcaaactta agccctattt ttgtctattc ttactgacaa
    31861 aaacgttttc ttaaaaaaga acaaaaaaac tggcgtagct cttttacaag ccacaccagc
    31921 ttttgatacg ttgtttattt tacatattca tgaacaagtt caacgacttc ttccatcata
    31981 tcgcattctt tcaatgcacg atcagcaagt tcagccattt tcgctgtgtc caagcgtttc
    32041 attaaactac gcgttttaag aatagatgtc gcactcattg agaactcatc tagacccata
    32101 cctactagca atggaacagc tgtttgatct ccagccattt ctccacacat acctgcccat
    32161 tttccttcag cgtgtgctgc atcaattaca tttttgatca aacgtaaaat tgatgggtta
    32221 tatggttggt acaagtaaga aacgcgttcg ttcatacggt ctgctgccat tgtgtattga
    32281 atcaaatcgt tcgtacctac agagaagaaa tctacttctt ttgcaaactt gtctgctaag
    32341 actgctgctg ctggaatctc gatcatgatg ccgacttgga tcgtatcaga tacttccttg
    32401 ccttcactta tcaatttttg tttttcttct tcaaagatcg cttttgctgc gcggaattct
    32461 ttgagtgtag ctaccatagg gaacatgata cgtaaattac catgaacaga tgcacgaagc
    32521 aatgcacgca tttgtgtacg gaacatttcg tcgccttgtt cagataaact gatacgcaat
    32581 gcacgatatc ctaagaatgg attcatttca tgaggtaatt gcagatatgg tagttcttta
    32641 tctccaccga tgtccattgt acgaacaact acaggttttc cttccatacc ttctaataca
    32701 gctttgtaag cttcatattg gtcatcttct gttgggaagt ctggagaatc catatacagg
    32761 aattcagtac ggtacagacc aactgcttct ccaccattat tatggacacc aactagatct
    32821 tttggtgttc cgatgtttgc agctaattca aaatgcttgc cgtcagctgt gacagtttct
    32881 gcatttttta atttttccca ttctgctttt tgtgctgcgt aatctgcacc tgctttttca
    32941 aattcagctt tttgttcatc tgtaggatcg atgattacat cgccttcgat cccgttgaca
    33001 gccaaaatat cgccttcttt gactttagca gtaatttctt ttgtcccaac gattgctgga
    33061 atttctaaag aacgtgccat gatagcagaa tgtgaagtac gtccaccaat gtctgttaca
    33121 aacgctttta caaaattgcg atctaattgt gctgtatcac ttggagtcaa gtcatgtgca
    33181 acaacgacca cttcttcgtt gatcattgat ggatttggta aagtaacgcc taataagtgc
    33241 gctaaaatac gtttagcaac gtcacgaata tctgcagcac gctcttgcat ataagcatta
    33301 tcttccattg cttcgaacat accgatatac atatccgtta cttcttttag cgctgattca
    33361 gcatttacgc tgttgtcttt aatattttgc ttgatttgac ctaccatttc tggatctgaa
    33421 agaaccatca aatgggcatc aaatacttgc gcttctgctt cacctaagct ttgcgctgct
    33481 ttttcacgaa tttgctgcaa ttcttcagta gatttagcta atgcagcatc caaacgagct
    33541 tcttcagccg cagtatcttc tactgttgcc ttactgaatg ttaaatccgg ttgaactagc
    33601 aggtaagctt ttgcaacggc tacaccgtca ctagcggcaa tcccttttag catttcaacc
    33661 attattcaga taagccttct ttcttcattg tttcaacgat tgctgccatt gcatcagctt
    33721 cgtcagcacc ttctgcagta atagtaacat cagaaccttg acctacacct aaagacataa
    33781 cgcccatgat tgatttcaag tttactgatt tacctttata ctctaagttt acatcagagt
    33841 taaatttgct tgctgtttgt actaatagtg tagctggacg agcgtggatc ccagtttctg
    33901 ctactacgtg aaattctttc ttttccatat taatcggtct cctttaagtg agtgttgttt
    33961 ttatgtgagg gttttctttt ttagtaattc agagaatcct aacaaatgcc accctttcat
    34021 ctgtaaagat taacattttt tttcgttaga tacaacagtt ttcattcaat ttcagataaa
    34081 agtattcaaa ttattccgga ttctttttca tccttttctt cacattttat aacaaacgtg
    34141 tttatttcct ttttcttgtc gctttctctt ctcctgacgt ttatcattct ttcctttgac
    34201 cctctttgtt ttcatatgaa acgaattact tgcttccctt tttattttta gtataataag
    34261 agtcaaaggt caggaatggt caatagagat ttcacctttt ctacctcata aatctcttaa
    34321 aaaactaggg gttattcttt tacctcatga aaaacgagat gtaaaatatg gatgtaccca
    34381 tttttgtgaa aggacgtgaa tgattcatgc tttgtcaaaa ctgcggcaag aatgaggcaa
    34441 cgattcactt gtatgccact gtcaatggtc agcggacaca actcgattac tgtcagagct
    34501 gttatcaaaa aatcaaaaat caacaaaatg gaggtctgac taatatggcc caaaatgatc
    34561 catttggatt tggcagtttg gacgatcttt tccgttccat gtctcgccaa atgcaacagc
    34621 aaggaaatta tgagcaaaca ccaccaacac aatttggcgg aaacaacaac ttcaatggtg
    34681 gtcagccacc tcaaggcaat acagaaggtt tattgggaga atacggaatc aatatcacag
    34741 aacaagcacg acaaggtgat atcgatcctg tgatcggacg cgatgacgaa atcaaacgag
    34801 tcattgaaat tttaaatcgc cgaaccaaga acaatcctgt cttgattgga gaaccaggtg
    34861 ttggtaaaac agcagttgtt gaaggtttag cacaaaaaat cgtagatggt gatgtaccgc
    34921 aaaaattaat ggacaaagaa gtcattcgtt tagatgtcgt atctttagtc caaggtactg
    34981 gtatacgagg acaatttgaa gaacgtatgc aaaaattgat tgaagagatc cgtcaagcag
    35041 aaaatgtgat cctattcata gatgaagttc acgaaatcgt cggtgctggt tctgctggtg
    35101 atggaaatat ggatgctggt aatattttaa aaccagcttt agctcgagga gaattgcaaa
    35161 tggttggtgc tactacctta aatgagtacc gcatcatcga aaaagacgct gctttagaac
    35221 gtcgtatgca acctgtacgg gtagatgaac caactgtcga agaaacgatc agtattttga
    35281 aaggtttgca aaaacgttat gaagactatc atcatgtaaa atatactgat gaggccatcg
    35341 aagcagccgc aactttatcc aatcgttacg tccaagatcg cttcttgccg gataaagcca
    35401 tcgatttgct agatgaaaca ggttctaaga aaaacttgac gatccaaatc gttgatccta
    35461 aaacaatcga gaaaaaactg caagaagcag aagaacaaaa agttttagct tctcgagaag
    35521 aagatttcga aaaagcagct tattatcgcg atcagatcaa taaacttcaa aaaatgaaag
    35581 aacgtcagct cactgaagag gaaacacctg tgattaccga aaaagatatg gaaaaaatcg
    35641 ttgaacaacg gacaggtatt ccagtgggtg aattaaaaga aaaagaacag acacaactga
    35701 aaaacttagc agatgacttg aaagctcatg tcatcggaca agataacgct gtagatcgag
    35761 tagcgaaagc gattcgccga aaccgtgttg gattaaacaa acagaatcgt ccaatcggct
    35821 ccttcttatt tgttggacca actggtgtag gtaaaacaga gcttgcgaaa caacttgcct
    35881 acgaactatt cggttcacaa gattcaatga ttcgtttcga tatgtctgaa tatatggaaa
    35941 aacatagtgt ctctaaattg attggttctc ctccaggtta tgtcggatac gaagaagctg
    36001 gtcaattaac agaaaaagta cggagaaatc cttacagtct tgtcttgtta gatgaagtag
    36061 aaaaagctca tccagatgtt ttgcatatgt tcttgcaaat cttagatgat ggtcgattga
    36121 cagatgcaca aggaagaacg gtcagcttta aagatacgat catcattatg acaagtaatg
    36181 ccggaactgg taaagtagaa gcaaacgttg gttttggtgc ggcacgcgaa ggtgttactc
    36241 gttctgtatt gaaccaatta aacaactact ttacaccaga atttctgaac cgttttgacg
    36301 ggatcatcga attcagtgca ttatcaaaag aaaacttgat gacaatcgtc acactaatgc
    36361 tagacgatgt caaccaaatg ctggcagcac agcaacttca catcgaagtt cctacgaatg
    36421 tcaaagaaaa attagttgac ttaggctatg atccatctat gggtgctcgt cctcttcgac
    36481 gcacgatcca agaacaaatc gaagatggta tcgctgaatt ttatcttgat cacccttcta
    36541 tccatgagtt gaaagcaaaa ctggataaag atggaaaaat catcgttact tctaaaccag
    36601 aacgtttggc taaagaatca gccgaagaaa cggctgaata aatccagttt tatagcgtga
    36661 gacggtattt ctttgagaaa tgccgtctca ttcttttttc agaaaagtca aaatactgtt
    36721 cattttataa aaaagaaatt ataataagag aaacaagcaa ttaaaaatac ataaaagagg
    36781 tgagcgccat gaaattggta aatgtaacaa acagccattc ccgtcttgta ttaaaccagc
    36841 ttgaaaacac tgatgcccat ctagtcaagg tctacacagc cggaaatacc actattgtct
    36901 acactgaagc ccctgaacat aatgaaattc tgctgatcaa tgacaaacgt aaaatccagc
    36961 cgaaagaaat cgaagatgct aaaaattatt tcttagataa aatcaaagac gccgtttacc
    37021 acgaggatga aatcaaagta atcgaattgg caggccttgt tgaaatctcg attccaaaac
    37081 atgaattagg cgctacatta gccgtgtaaa caaattttga acataataat taaagagcgt
    37141 gggacaaaag taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca
    37201 gaagcaactc cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga
    37261 attccagctt atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt
    37321 tttgattttt ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct
    37381 ttatttttaa ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg
    37441 tttttgtccc agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc
    37501 tgtttttttc tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta
    37561 atcatatgta cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat
    37621 atagtaaaaa gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg
    37681 tttttgtttg gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca
    37741 tcaaactttt taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt
    37801 tctcgaataa gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac
    37861 tcatcttctc atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag
    37921 acatgattac ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta
    37981 gctgaccaac agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg
    38041 ctccttcttg aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt
    38101 ttgcagtcac tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt
    38161 ttagttttcc ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga
    38221 tgatatcgcc tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga
    38281 cattgcttaa tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga
    38341 atgttccaga acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg
    38401 cttggatctt gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat
    38461 cttgtgtttt gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct
    38521 gtacaccgaa gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa
    38581 ttttttcttg attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt
    38641 gttcatataa ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca
    38701 attctactaa tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt
    38761 agatatcata aagaccttct agccctttac ccataccgat tggccaattc atcggataag
    38821 attcgatatc taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac
    38881 gatctagttt gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata
    38941 atttctttgt ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca
    39001 cggccatcaa agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga
    39061 tatttactcg tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac
    39121 gttgtttctc gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta
    39181 cggtcccggc ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt
    39241 tcccagcatc cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta
    39301 attttggatt attcataacc taactccatt tcttccattc aattatttat attttctcta
    39361 ggcacagatt gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc
    39421 gtgtagcagt ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc
    39481 ccacccaagc caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca
    39541 ggatagcttt ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca
    39601 gcgaatgaaa aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag
    39661 aaaagacgtg ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat
    39721 cttcttggct cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc
    39781 gcttctgttt cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc
    39841 atttcttcag aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc
    39901 ggaatcatgc ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc
    39961 agatcactgt caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat
    40021 tcgtgttcat cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct
    40081 ccgacgattt cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca
    40141 tctaataaaa tagccatctg attctgcgtc tttttcattt catataacag atcatcaata
    40201 aagaccgttt caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca
    40261 aagccgaatt tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta
    40321 tctttgtctt cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca
    40381 ttttcttcca cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact
    40441 tctcgggcaa ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta
    40501 ttcaatacgc cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct
    40561 tcatcatcaa aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc
    40621 cagacgaagg gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg
    40681 acttcttctg atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat
    40741 acataggtca aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca
    40801 cctaataccg gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg
    40861 ttgaccaaag tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt
    40921 tttaaaagct tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta
    40981 ttgatcgaaa caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg
    41041 atcaataata aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca
    41101 ttctctcctt ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca
    41161 cgttaaaatg gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac
    41221 ctacgaggtc gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt
    41281 gattatatag gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc
    41341 aagcaatccg gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat
    41401 tttcagtgat ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat
    41461 tagcacaacc acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa
    41521 tgcaggtgct agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat
    41581 gaccccaaat aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt
    41641 accaacgaat aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag
    41701 ttgccccaat aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt
    41761 tggcactagt ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat
    41821 tactacggtt gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact
    41881 aaggaattgc tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat
    41941 atctacttcc ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt
    42001 gatgaaatca ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac
    42061 actaagtaaa atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca
    42121 tattcttttg atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag
    42181 aaagcctgca atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa
    42241 aataaagttt atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga
    42301 ccaaaacatt agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct
    42361 tactagtata tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt
    42421 gccatacttt ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc
    42481 atgaaagttg tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc
    42541 aggcatcaag tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat
    42601 gaagcccatt gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta
    42661 cgtcttttgc ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa
    42721 tacagacacc aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat
    42781 caaggctata atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa
    42841 ctaggatata cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg
    42901 aaaaagactt tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa
    42961 atctctcact ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg
    43021 ttataccaaa tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca
    43081 accagaatct tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt
    43141 ttgctgtgca tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc
    43201 ttgatatcca agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa
    43261 gctagattac aataatagga aacccccttt tctctgatca ttgctattac attgaaccag
    43321 tattttttat gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat
    43381 tcagtgacta atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc
    43441 atggtatctc gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca
    43501 aactctcctt cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg
    43561 tgtttgggtg ttcattacac cgacatcata attattatat caataaaaat gagctttgcc
    43621 tagaaaggat tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc
    43681 ttaggaaaaa cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg
    43741 gtaaattctt gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc
    43801 agatgactaa aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc
    43861 tgcttttcaa atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt
    43921 tctggctttg tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc
    43981 attggaaatg tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac
    44041 gctccagaat gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt
    44101 tttgttttta caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg
    44161 atcggacaag tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc
    44221 attgcttggt tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca
    44281 aagattttcc tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact
    44341 actcgcatca cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc
    44401 gcgcctgtcg tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc
    44461 atttccccat caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt
    44521 gagtaataac caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc
    44581 tcttcaattg taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact
    44641 ctggtttgct gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc
    44701 caaggcaggt ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt
    44761 gtttctttat ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat
    44821 gaatcgctgg atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag
    44881 atttcttttt ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac
    44941 taaataatca tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg
    45001 gatcacttct tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc
    45061 tttttggctt tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg
    45121 aacttggtct tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc
    45181 tttttgctgc atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt
    45241 gcgcacataa ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc
    45301 tacgatttta tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc
    45361 tttttccgat cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc
    45421 tccgctactc ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa
    45481 tcgatagcgt actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac
    45541 gcgatagaaa gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct
    45601 cgaacatata ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga
    45661 ataaaccaca gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg
    45721 acaaacggtt cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt
    45781 caaacggttg atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat
    45841 cacgaaagca actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa
    45901 agatcgcgta gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca
    45961 cttagcttat caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa
    46021 attcaaacca gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc
    46081 ttggcattat agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat
    46141 tgaagctggt ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt
    46201 tcaagaacct cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga
    46261 tgcacccatc tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat
    46321 cggtgttaaa actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca
    46381 aaaaaacgca atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca
    46441 aaatgtccag gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg
    46501 aaaagaagcc attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt
    46561 ctttcaactc aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga
    46621 tgtccagcct aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag
    46681 tgtagcaaaa caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga
    46741 tgcaaaaaaa aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc
    46801 agagcaagtt ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga
    46861 tccaccacgt acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa
    46921 aaaacttgtt tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag
    46981 taaaaagttc aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg
    47041 tgaagttgtc gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc
    47101 tgccctgtat caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata
    47161 ctgtacttgt gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga
    47221 catagttttt gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata
    47281 aaatgcattt gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg
    47341 atcctgcgga agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc
    47401 tatttttgtg atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg
    47461 tttggtaatg tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat
    47521 atttaatgtt cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa
    47581 aggctcaaag ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt
    47641 agcttcgata ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc
    47701 tgctgatgtt cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc
    47761 tggaatgcta aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat
    47821 ctttttcatg tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta
    47881 acacaaacat tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat
    47941 gcagattata acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat
    48001 atgatctttt tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa
    48061 ttatcaattt ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt
    48121 aaactaatat tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg
    48181 gtttgtgtta taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc
    48241 ggaaaatatt actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg
    48301 ccgatgtgga ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac
    48361 aacaacaaaa aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat
    48421 tttatatggt ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt
    48481 acacttttag ttattttctt gtcagtgctt atgtatcagt tgtggctgca tcatcgcctg
    48541 gcaatgtggc tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat
    48601 tcaatttatt ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa
    48661 aagtgccacc taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact
    48721 atttttttct agtacccact tgttgttcca tactcattat tctggtcata accataacca
    48781 ttttgttcct gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac
    48841 tgacttgtat cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg
    48901 ttttctgaat aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc
    48961 gaatcgccat cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta
    49021 ggtaatgtat cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga
    49081 agatcatttt ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc
    49141 aactggtctt gtttgattgt ttcaaacgcc ggggtgtagt tcgtcgctac atcaagcatg
    49201 tcatcccaat ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa
    49261 ttgcttacgc catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga
    49321 cgacgttgtc gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa
    49381 cctttttctc cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag
    49441 tcaattttat tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc
    49501 aaattgatcg ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg
    49561 gccatttcga caccaccaaa tgcgtaggca tgatttaatt tatctaccgt tccataacct
    49621 acgatattcg tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga
    49681 ttaactgtta ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg
    49741 gtatctaaac ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca
    49801 gaacgtttgg aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct
    49861 tctcccataa atctgattcc gtatgctgat aatccggcaa tcagcaaaac gaatacccct
    49921 aacaaggtta atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct
    49981 aaaatattgt ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact
    50041 ttaaaaaaca tttttgtgta acgattatta ttttttaaaa tttctcaaaa ttcttaacta
    50101 tcataccata ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat
    50161 ctttttttat gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg
    50221 cagtaaaatg atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga
    50281 ttatcattcg atttcacgac aatttcttcc tatgactggt ccttttgtta aacccatctt
    50341 ctattcgttt tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgattgc
    50401 attaccatca tcttttaatg gatagatatg ctcctcttca aagtaaaacg attttctttt
    50461 aaagtaatag aattgacatg gtaatgagta tgtatactag tggtttcaca aatggtcttt
    50521 tattcaacat ccaaaaactg aaatttcttt aaaatccatt actaacgaag aaaatgttcc
    50581 ttgcttgata attagttagg acatgtcaag tgaacaaatg catttactta gccaaaaaat
    50641 ctaaaaactt cataaataga caaaaagaca cgactctcta agaagtcgtg tctttattca
    50701 acaaattatt ataatttagt cacttcagca gcttgaggtc cgcgtgcacc ttcaacaatc
    50761 gtgaattcta cttcttgacc ttcttctagg ctcttgaagc cgtctccttg aattgctgag
    50821 aaatgaacaa atacgtcatc tccgccgtct actgtaataa acccaaatcc tttttcattg
    50881 ttgaaccatt taaccgttcc gtgttccata aatgtatatc ctccaaaaaa gttcttgcaa
    50941 gtaaccttgc aagaacatta tacccaattt agaaaacgct agcaagcttt cgctctttta
    51001 ttttccaaat tatttttaag actgatatga cggaggaaat ggttattatg ctgcttcgaa
    51061 acgtttgttt acttcatccc aattaataac attccagaat gctgcaatat aatctggtcg
    51121 gacattttta tatttcaaat aataagcatg ttcccaaacg tccaaaccta gaattggtgt
    51181 ctttccttcc atcaatggag aatcttgatt tgcagtagag gtaatagcta attttccatt
    51241 ttccattaca agccaagccc atccagaacc aaatcgtcca gcggctgctt ttttgaattc
    51301 ttctttaaaa gaagaaaaat caccaaaagc ttcattaatc gcttctttta tttctcctgt
    51361 aggttcgcca ccagcatttg gtgccatgat ttcccagaaa aatgaatggt tagcatgtcc
    51421 gccaccattg ttacgtacag ctgtcttgat atctgttgga atagcgtcca tatcagataa
    51481 taattcttct attgtttttt cgcctaattc tgggtatttc tcaattgctg aatttaaatt
    51541 cgtcacataa gtattgtgat gcttatcatg atgcagatgc atcgtttctt cgtcaatata
    51601 aggttccaat gcgtcatatg cataaggtag atctggtaat gtgtaagtca taaaaaaatt
    51661 cctcctcaat gttttcttaa taatgtctac attacaaaga gtaacaaaga gaaggaaatt
    51721 cttcaattgt tatgcctagt cgtctttttc actttgacct acttcttctt caaaaacctc
    51781 agtttcttta aaaataaaca acttgctgaa aatatagttc gcaaggataa caataatttg
    51841 agtgatgatt ttcgccacat actcattgct gttcataaca ttgatcaaca ataccattgc
    51901 ccccatgtcc aatccgtaag aaaggatacg gtagaagaag aacttaccgc attcaataag
    51961 tgcatgtgta aaattcggtg attttgaatg gaatacccaa agtttgttcg ttacaaaagc
    52021 aaacaataca gaacaaaacc acgaaataga attggataag gccatactta aatcaaaata
    52081 ttggcgtgcg agagcaaaac taacaaaatt tacaatcgta gccaaaccac caaaaaagag
    52141 atagactagt atctcccaat atccttttgc ttctaaatat ccttttaact gtcgatactg
    52201 cttcatgagc caacctacct tattaaaaat tcttatttat catactctat atttcaataa
    52261 ctttcatctt tatttaaaaa aattaatttt ttttgatcct ttgcctttgt ctctgcttgg
    52321 atcgtcacat gtgtaatacc atattttaat tttaataaag aaattatacg aggatatatc
    52381 ttttcaatct cgcttagtag ctgatcttca aaatcaatat gtccgtcgaa aataattatc
    52441 ttttcgctat actgccaaat atggacatga tgaacatcct ttaccccatc gattgcttca
    52501 atttcttgtt ccattttttc atagtcgata gcaggtgcac tttgtatcaa aatagcggca
    52561 gcttttttga tcaccattat cgcttctatc aatatatata tcgaaatgat cagtgtaata
    52621 actggatcga tccagtagat atcgaaaaaa cggatcaaaa ttgctccgat aatcacactt
    52681 accgaagaca gtgtatcact taacaaatgc aaataactag attttatatt caaactttca
    52741 tctgcttctt gtcgtaataa taatactgag aacaaattcg cgagcaatcc aataactgct
    52801 actgttagca tcaaatgact atttatcttt tctggtgaag tgaaccgctt gaatgcttca
    52861 acggttaaaa acaaggataa tatgatcaat atgaatgagt tgatgaaagc actcaatatt
    52921 tctaaacgct ggtagccata cgtacgtttt ttatttgctt cttttcgggc cattttttgt
    52981 gccccgtaag aaaaaataat tgccaatgta tcactaaggt tatggaaagc atctgaaaca
    53041 agagacaaac tccctgacat aatccctcca ataaattctg ataaagtgat caccgcattc
    53101 aaaaccacta ctccaaatag tttttttgat gatctctgtg gctgtgtagg cactattcct
    53161 tcctccattt tatttatatt ggatagtctt tagacaagaa taacattttt ttgatatttc
    53221 accaatcatt cagctgtttc cttaaagcac ttgtatttca cttgatactt cgctagaatt
    53281 gcaagtgatg acactactag atgaggagta cttatgacaa caaaacaatt aattatcagt
    53341 tcctttatac gattcaaaga cctaaaggag gcgagaaatg tcccattttg gaaagccatt
    53401 gtctatcttt tagttttgag tataattatg gctttgccaa tctcttttca agtttttcaa
    53461 gtcttaaaca acattcaatt ggatgggcaa aagattgcta ctcgcattcc tgattttaca
    53521 atccaaaacg ggcagcttca aaccgaagaa aaagaaggat tcatttacca aacaaactcc
    53581 attatcttca catttgatcc agagggaaaa cgaacagaac aggacatctc tactgatttg
    53641 atggggaatt ttgttagtgt cgggatgtta aaaaacaaac ttatcatagc tcttcctaat
    53701 acaggcacga cttctgcttt gttgaacaat aaccaattag aacttcccta caccaatgaa
    53761 tctttaaaaa acttaacagg taagcagctc cgttcttttt taagtgaagc atctatacca
    53821 ctttggataa aagtcctgac atttatattt tctatttatc ctagttttct gaatctgctg
    53881 atcactttgc tatttaccaa cattgctgct tttctctatg cacgctttcg actaacaaaa
    53941 gctacttttc ttgattgttt aaaaacattg atctattcaa tttcattgcc tactataatt
    54001 gcaacaatct tgatggtctt tcttccttct tttgacacta gcgccttcat tgcttttgca
    54061 ggaatcttta tttttgctca agctgtcaaa ggatgggcaa aaatatcgat caattaagaa
    54121 ataaagacaa ctcgttattt ctacttcaat agaccctttt agaggttgtg acattttttt
    54181 gtcacagccc cttttttatt tgaaaaatat ttctttctca aaaaacaagc aaggcgcttc
    54241 aaagttcttc ttgtatgcgc tacacttaga gtaaagaaag gcgggaaatt aactatgtca
    54301 gaaagaatca tttatgcaga tcgtccattt cgtgatgaat ttatcgaaga aatccatact
    54361 atagcgcctg gttatatctt taaaacagaa ttacaaccag aggatctaca aaatgtagag
    54421 atcagtttgg gctggaactc agactaccaa gaaaaactat tagcctcttc taccttgaaa
    54481 tgggtccaat ctatctcagc tggtgttgat cagctacccc ttgagacttt ttcaaagaaa
    54541 catattttac tttcaaatgg tagtggcatc cacagtgaat cgattacgga gcatgtctta
    54601 ggaatcatcc ttgggtattc tcgtggtctt tttcaagcac aaaaggcaca gagtgacatg
    54661 aaatggttag gttcatcggt ccattaccaa tctatccaag ggaaaaaaat actaatcatt
    54721 ggaactggcc atattggaaa aatgattgca aaaaaatgca aagcattaga catgaactgc
    54781 tatggaatca atacatccgg tcatccagca gaaggaatgg aaaagacttt ttctttaaat
    54841 caactaaagg aagttcttcc tgaagtagat ttcgtagtca atatcttacc tctaacggag
    54901 gaaacaactg ggctctttga tcagcatatg tttgaacact ttgatccaaa atctgtattc
    54961 atcaatgttg gccgtggtgc ttctgttaag acacaagatc tggtgcaggc tttaaataac
    55021 cagcaacttt cctttgcagc attagacgtt tttgaggaag agcctcttcc acaagatcat
    55081 cctctttgga aaatggacaa tgtactgatt acttcccaca ttgcaggttt gacacctgat
    55141 tttcaaaaga aattaatggc tattttttta accaatctga aaagctactt tgccacccat
    55201 gagcttcaaa ccaatcaagt aaaattgtct gcaggatatt aatagctgaa aacaatattt
    55261 tcaaaagaaa aaagagaagg tgacaaaaat tgtgtcaccc tctctttttt tattgttttt
    55321 taaagtgata ggttggctcc tccaggttgg gattataatc aacaagtaaa tcatatcctt
    55381 taaagaacca ttcatctgct tcctcgataa aaaaaagtgt cccatcaatt tccactttct
    55441 ttacaggatg ttctggctgt tcaactgaca ttccaatcga aaaaccatca tgaacctctg
    55501 ttttgccgta gacttttcca aaaaaacgta ttccgtaagt ttctggcaga tcaatttctg
    55561 agtcaaacca cttttgtgct tttggcgtta ccgttaattt catcccaaca acctactttc
    55621 tcttttcttt ctattgattc atcttatcgt ctagaatatc cacatcgcct gtctctccaa
    55681 taaccagtag attatcattt tcacgaacat attcatctgc tgcaggagaa gcgataaccg
    55741 tttgatctac tcttcgtatg gcgactactg ttaagtcaaa acgttgcctg aaatttaatt
    55801 cagctaatgt tttgttgaaa aatttaggat tggagactcg gatttctgct aaagaatatt
    55861 tatctgacaa ctctaggtaa tctaaaatat tctttgacac taaattatgc gcaatcctta
    55921 ttcccatgtc tcgctcggga tggacgacgc gatcagctcc gatcttttca agtactctgg
    55981 catgatactc attttgggct tttgctaaaa tatttggtac gcccatttcc tttaccatca
    56041 gagtaactaa gatactggct tgaatatctt ctccaatagc gacaattaca tgatcgaaat
    56101 ttcttatgcc taatgatcga agtgtcattt catcctgtgc atttgccaca acagcatgag
    56161 tggctatatt catatattca ttgacccgat cttcattact atcaatagcc agtacttctt
    56221 gaccagcttc gatcagtgtt cggcagatac ttccgccaaa tcgtccaaga cctacaattg
    56281 caaaattttg tttcaccgtt ttcctccttt agtatcttct tttctcagta taacaaagcc
    56341 tttacaaaaa caaaagcaag catcattatt gtaaaaaagc caagaaaaat aatagttatt
    56401 ttctcagctt ttgattatcc tctttatcat cgtttttatt tcaccaaacc atgtttgaat
    56461 gcatagatag cggcttgtgt acggtcttcc acttctagct tagccaatat attggaaaca
    56521 tgtgttttta ctgttttcag tgtaataaac aattcgtccg ctatttcctg attacttttt
    56581 ccttcagaaa tcagcattaa aatttcattt tcccgattcg ttagttcttc atgtaataca
    56641 ggctcatttc gccgactcat tttttccatc atttttgttg tcacttctgg ttctaatact
    56701 cgctcgccac gttgcgttgc ccttatagca tcagcaattt catgtgcggt agaggtcttc
    56761 aacaaatacc ctgctgcacc tgcttcaatg gcaggataaa ctttttcgtc atcaataaaa
    56821 ctggttacga taatgatttt agcttctggc caatctttta agattgcttt tgttgattcg
    56881 ataccatcca tttcttccat gactaagtcc attaaaatga cgtccggtcg aagtgtcatc
    56941 gccttctcat agccttgtcg tccattttca gcttctccaa ttacttcaat gtcttcttga
    57001 atagataaat aaaaagagac gcctaagcgc accatttcat ggtcatctac taataaaact
    57061 tttatcatcc ttatctatgc ctccttcatc aaagggacct tgatttctac acttgtacct
    57121 tgacctttaa aactaatgat ttttactgtt ccaccaatac cagctactcg ttctttgata
    57181 ttgttcaatc cataacttcc cgttttcgtt tcattcatat taaaccctgt tccgtcgtca
    57241 ataatacgta acaaaagatt attgtctatt ttgtgcaaat atacctctaa ttcattggct
    57301 ttagcatgtc ttaatgtatt tgataacaat tcttgaacga ttcggaacaa gtgatcctca
    57361 atggaactag atagttttac atcttctaca tcccatttca gcgaaatctg gattttattt
    57421 tgcaattcct tcaataactg ctcgatacct tgctttaaac ttttctcttc taaattgact
    57481 ggacgtagat gaagaagcag cgcacgcatc tcagactggg aagcgttgat gatctctgct
    57541 accattttca actgcttgcg aaacatttct ggtgtctcgc ttttttctgc ttgctccgtc
    57601 aatgccgaca tcatcatcat tgctgcaaac aattgttgcg aaactgaatc atgcagctca
    57661 cgggctaatc gatgtctttc taattctaaa atttcttctt tggtttgtcc atctacatat
    57721 tggggacggc tcgttacaat ttgtaattca ctagatactt caatcatttt ttcttttatc
    57781 ttagtgattt ccttatcaat atctttgatg tataaatcgt ccgatgcact tgggatcgga
    57841 agaagcaaca atttggattc ataattgcca gaagaaagtg cacgcagctt ttcttcgatt
    57901 ttcccgtatt ttgtcttttg aataagtgat aataatagaa agacaattaa tcctacacct
    57961 aaggaaatgg ctaacaaatg gaagataagc ggaacatata ataaacgtgc acgcagtaat
    58021 tctccccacc aatgactttg attgctggca taaaaataag tgaaaagtga aaatagaata
    58081 ataaggaaag ccgcaatccc cgaataaaca gctagcatcg ctctggatat ttttcccatc
    58141 atattcgtat cacctcgaca tctccaatca aggtattagt aataatcttc aatctacgag
    58201 ggttttcatc ataatcactg ctgtagattt tgatttgttc atttttcaaa gaaaattgct
    58261 cttcttcaaa caagacattt cctactaatg tcgcatgctc taatcggatg gctacgccca
    58321 aaggtactaa gatccgtgtt ctgcctatcc cttttcttac aatcacaata ttatcatctt
    58381 ttggcaaaag cgtattgccc aaatctatga tagtatcacc agaaatgacg gcgatgttga
    58441 tatcgtccca ttcatagacg tcgtttccaa tacgttggtt accgaataat tgctgtcttt
    58501 tccgctcatt attatgtgtt tttaattgct ctgtttggat catcatgatc tgtttctttc
    58561 gccaaaaagc atttttggtt aaatctatcc ctgaaatctc tacacctttc agaccaataa
    58621 ataaaacggc aaatacagcc atcatccata aagcaggact gttgactaag ctaaaaaaaa
    58681 tgaccaaact tcctaatatc aattgaaaat tttgaaattt agtacgagga tgtttgcgca
    58741 tagctagata tatattgaaa ataccgaata tcactaaaag caaaagtcca gtattgttta
    58801 cgatttgcca aacagcaaat aacagcagta gtgcttcaac taccacaaaa aaacgccaag
    58861 aactattcat cgctctttct ccctcctgac acattctcat ttcatcatac ctattttttt
    58921 aactgttggc tataggactt tagatgtaag atcgtttgtt gaaatctaaa atacacaaag
    58981 agaaaaagat ctagagtgta tcatcagtaa gatgaactct ctagatcttt cagtcagact
    59041 acgcttgttc tactttgacg attttaacta acatatcgcc acctggagta gcaatagtta
    59101 cttcatcatt tttacgtttg ccaatcaatg cttgcgcaat aggagaatca ttggaaattt
    59161 tgccagaaaa cggatctgct tcagcgcttc cgacaatcgt gtactcttct tcttctccat
    59221 ctggtagttc gatgaatgta actgttttac caattgatac ctcatctgca tctacaccgt
    59281 cgttatcgat gatttgagca aaacgaatca tattttcaat tgttgtgatt cttccttcaa
    59341 caaatgcttg ttcatctttc gctgattcat attcagagtt ttcggaaaga tctccgaaac
    59401 cgcgggcaat tttgattcgt tcaatgattt ctccgcgttt gactgttttt agttcttcta
    59461 attctttttc taatttttct ttaccttcta gagtcattgg ataaactttc tctaccatgt
    59521 gtaacgtctc cttttttcga taaaataccg caaaaggtaa tcttttgagg aatatagtcc
    59581 aaaaaagatt accacgcttt tcttcaaatg taaatgtttt tctaaaaatg atcaatgtct
    59641 agagcctgtt tttctaagaa ttatttacat atttttccac taattccata tgttcttcat
    59701 aagttttagc gaaataaaca tttccagtgc tcgtatctgc tacgaaataa taatagtcat
    59761 tttctgctgg ttctaatact gccttgattg cctcttcgct tggattgtca aaaggtccag
    59821 gtccatagcc ggtattggta tacaaattat atggtgaatc tacagctgta tcttcatacg
    59881 tcactaattc tttgtgttca cctaatgcgt ataaaacaga gatatctgac tgaagtggca
    59941 tatttgcttt gattcggtta aagaagactt gtgcaatgtt tttacgatca ttttctttta
    60001 cgccttcttt ttcaactaga gaagctagag ttaaaacttc ttgtacagtc aaatttttct
    60061 gcttgatagc tgaataatag ttttgcatga ccgtattcgt cttgttcacc atttcaatca
    60121 ctagatcctt aagcgaagta ctcgtgtaat aatcatatgt ggctgggaat aagtaacctt
    60181 ccaaacggta ttttgtgttc tcagctttag atgcactttc caacagttct gggaattttt
    60241 ggtgcaactc gttaaagaaa gtctcatcat tcactaaatc taaaaattct ttcttgtctt
    60301 taccagttac cttcgctacc cgttcagcaa tctgatcgat atcgtaccct tctggaatgg
    60361 caattttccc atctgcaact tttgttggct cagaagtacc gccttcttgc aactgtttgc
    60421 caatctcgtc aagagtcata ttaggcgcta actgataata gccagcttga aagccagtta
    60481 aatttttaaa ttttgtataa taattgaaaa caatcccgct tttaataata ttgtccttct
    60541 ccaaaatctc accaatctgc ttgttagaag agccactcgg gatctcgact tggactaatt
    60601 ggtcatctga tttatctaaa ggcttcaacc cgctatctac ataacgatac acggtaaatc
    60661 caagaaatcc gccaataatc aataatgtaa gagcaatcac taaaatgatc ttcctcacaa
    60721 tcttatcttc ttttttacgc agatttctcc gcttgtcgat tatatctgct gtagctgcct
    60781 tactggcttc ttttttatct ttacttgggt gttgtttgtt tcctgacaaa acaactcctc
    60841 cttactatca agttcgtaac tttattcatt atacatgata tttgaattgg cgtgaatgat
    60901 tttatttaaa tattctatca aaaatgtaag tagtaaatcc attactataa gattaaaata
    60961 aatgttatca agatacaagc tattttagct ttattctttc tgtaaaaaag aaaggagact
    61021 aaataaacta ttatgaaaaa aggaaaacat gctaaaaaaa gaaaatattc atttttaatg
    61081 atcgttgtcg tgttgtttag ccttagtgcg ttctctacag gaactattct tggatggcaa
    61141 ttttacaata atgaacactc tcagatagtt gcccatgctc aatcagctca aaaaaagttg
    61201 atactgacta ccaatgaaca acagctcgtt gaaaaaatcg atcaactatt aagaaaaaat
    61261 gattatattg gctcaattta cgtaaagaaa aataatcgca tcatcatgga aaaaggctat
    61321 ggctatgcga ataaactcac gcaacaacca aatgatcctt ctctttatta tcagattggt
    61381 tcggttcaaa aagcattgac tactcttttg attttaaaac aagtcgagcg tgggcacctt
    61441 tctttagata ctaagctggc tcaattctat ccacaaattg ctggcagcca gcaaattact
    61501 atccgtgact tgctgtatat gcgctcaggc ttgcaccgaa ctgcttctcc aaccataccg
    61561 atgactgatg aagaagtcgt tcaatttgcg attcagcact taaaatttac agattatcag
    61621 acctctcatt atgattcatt gaactttacc atattgactg gaattttgat caaattaaca
    61681 aatcaaccgt atgaaaaatt attgaccgat gatatcatcg aacctttagg attacagcat
    61741 acaaaatttt atgaccaagt caaaaattca tccaatcatg cactatccta tcaaatgtct
    61801 aaagaggatg attactttaa accactgaca gaatctgaaa cagagatcca taacgaatta
    61861 gggactggta atatcagtat gtccgtctat gatttaaata cgctgtttac acaagtttta
    61921 tctggaaaac tagtttccaa agacttactc ttctcattat ggcgtgaaaa tgcgaatgga
    61981 catccatata gtggtggggt atatagcgga gataattata tcctgtcgca aggaaatatc
    62041 gactcttttg atacatctgc tatatttagc aaagattcac aggatgctat catcatggaa
    62101 agcaatataa aagctgacaa aactatcaaa ctgcctactg ctgatctaag aaaccaaatc
    62161 tatgacctga tggaaggaac tactaagttg gcaaatagct gatatgatga aaagatagca
    62221 aatactactt ctttgaatag catttgctta aaaaaagtac tcgactaaac gtaggaaaga
    62281 gtgtgggaca aatctaaaaa tcaatttttg ctacagactt taattccaaa taaacagaac
    62341 agaaagaggt gttcttcttt ctgttctgtc caaattattc taagatttct ctttaccaac
    62401 attatttgac aaagggccta ttaaatctaa aatagtatct ttagattgct aaaatatgtg
    62461 ctagcaaatc attcatggct tgttcttatt tttaatttta tgcaataaaa aagaagaaca
    62521 attagcgtga ttatagaaat aaataatgag attttagaaa aaaatgtgta tgaatacctg
    62581 attgttactt tatttattcc actttcactt tttactgtaa caaaaggacc tccttgtttt
    62641 agtactgtag cattttttga attaacagta accgttaatc ctttataccg gaatacaggt
    62701 aaaactattg ttttgccttt tgaagattta aacgtaaatg taatttcatc tttatttgga
    62761 gaaaagctac tataaataac ttgaccat
//
LOCUS       DAZBTF010000008        62490 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000008 DAZBTF010000000
VERSION     DAZBTF010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.008"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            320..640
                     /locus_tag="ACHGNS_000560"
     CDS             320..640
                     /locus_tag="ACHGNS_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFN6936541.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            794..1699
                     /locus_tag="ACHGNS_000561"
     CDS             794..1699
                     /locus_tag="ACHGNS_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6936542.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1696..2469
                     /locus_tag="ACHGNS_000562"
     CDS             1696..2469
                     /locus_tag="ACHGNS_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFN6936543.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2650..4074)
                     /locus_tag="ACHGNS_000563"
     CDS             complement(2650..4074)
                     /locus_tag="ACHGNS_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFN6936544.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            4285..4731
                     /gene="nrdI"
                     /locus_tag="ACHGNS_000564"
     CDS             4285..4731
                     /gene="nrdI"
                     /locus_tag="ACHGNS_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFN6936545.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(4796..6778)
                     /locus_tag="ACHGNS_000565"
     CDS             complement(4796..6778)
                     /locus_tag="ACHGNS_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFN6936546.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6792..7688)
                     /gene="murQ"
                     /locus_tag="ACHGNS_000566"
     CDS             complement(6792..7688)
                     /gene="murQ"
                     /locus_tag="ACHGNS_000566"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFN6936547.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(7691..8752)
                     /locus_tag="ACHGNS_000567"
     CDS             complement(7691..8752)
                     /locus_tag="ACHGNS_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFN6936548.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(8963..9490)
                     /locus_tag="ACHGNS_000568"
     CDS             complement(8963..9490)
                     /locus_tag="ACHGNS_000568"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFN6936549.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(9557..11059)
                     /gene="eat(A)"
                     /locus_tag="ACHGNS_000569"
     CDS             complement(9557..11059)
                     /gene="eat(A)"
                     /locus_tag="ACHGNS_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFN6936550.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(11317..12438)
                     /locus_tag="ACHGNS_000570"
     CDS             complement(11317..12438)
                     /locus_tag="ACHGNS_000570"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFN6936551.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            12564..13505
                     /locus_tag="ACHGNS_000571"
     CDS             12564..13505
                     /locus_tag="ACHGNS_000571"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFN6936552.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(13655..14551)
                     /locus_tag="ACHGNS_000572"
     CDS             complement(13655..14551)
                     /locus_tag="ACHGNS_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFN6936553.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            14820..16160
                     /locus_tag="ACHGNS_000573"
     CDS             14820..16160
                     /locus_tag="ACHGNS_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN6936554.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            16378..16821
                     /locus_tag="ACHGNS_000574"
     CDS             16378..16821
                     /locus_tag="ACHGNS_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFN6936555.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(17325..18743)
                     /gene="nhaC"
                     /locus_tag="ACHGNS_000575"
     CDS             complement(17325..18743)
                     /gene="nhaC"
                     /locus_tag="ACHGNS_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFN6936556.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(19146..20369)
                     /locus_tag="ACHGNS_000576"
     CDS             complement(19146..20369)
                     /locus_tag="ACHGNS_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFN6936557.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(20400..21239)
                     /locus_tag="ACHGNS_000577"
     CDS             complement(20400..21239)
                     /locus_tag="ACHGNS_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFN6936558.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(21256..21936)
                     /locus_tag="ACHGNS_000578"
     CDS             complement(21256..21936)
                     /locus_tag="ACHGNS_000578"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFN6936559.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(21938..23011)
                     /locus_tag="ACHGNS_000579"
     CDS             complement(21938..23011)
                     /locus_tag="ACHGNS_000579"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFN6936560.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(23067..23305)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(23348..23581)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23658..24329)
                     /locus_tag="ACHGNS_000580"
     CDS             complement(23658..24329)
                     /locus_tag="ACHGNS_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN6936561.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(24416..27064)
                     /gene="mgtA"
                     /locus_tag="ACHGNS_000581"
     CDS             complement(24416..27064)
                     /gene="mgtA"
                     /locus_tag="ACHGNS_000581"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFN6936562.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(27406..27580)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            27753..28190
                     /gene="ndk"
                     /locus_tag="ACHGNS_000582"
     CDS             27753..28190
                     /gene="ndk"
                     /locus_tag="ACHGNS_000582"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFN6936563.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(28212..29078)
                     /locus_tag="ACHGNS_000583"
     CDS             complement(28212..29078)
                     /locus_tag="ACHGNS_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFN6936564.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLSHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(29238..31331)
                     /locus_tag="ACHGNS_000584"
     CDS             complement(29238..31331)
                     /locus_tag="ACHGNS_000584"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFN6936565.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(32080..32703)
                     /locus_tag="ACHGNS_000585"
     CDS             complement(32080..32703)
                     /locus_tag="ACHGNS_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936566.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(32862..34259)
                     /locus_tag="ACHGNS_000586"
     CDS             complement(32862..34259)
                     /locus_tag="ACHGNS_000586"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN6936567.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(34291..35262)
                     /locus_tag="ACHGNS_000587"
     CDS             complement(34291..35262)
                     /locus_tag="ACHGNS_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN6936568.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            35636..36061
                     /locus_tag="ACHGNS_000588"
     CDS             35636..36061
                     /locus_tag="ACHGNS_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936569.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(36549..37250)
                     /locus_tag="ACHGNS_000589"
     CDS             complement(36549..37250)
                     /locus_tag="ACHGNS_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFN6936570.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(37266..37706)
                     /locus_tag="ACHGNS_000590"
     CDS             complement(37266..37706)
                     /locus_tag="ACHGNS_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFN6936571.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(37706..38281)
                     /locus_tag="ACHGNS_000591"
     CDS             complement(37706..38281)
                     /locus_tag="ACHGNS_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFN6936572.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            38522..38731
                     /locus_tag="ACHGNS_000592"
     CDS             38522..38731
                     /locus_tag="ACHGNS_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFN6936573.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
     gene            complement(39129..39641)
                     /locus_tag="ACHGNS_000593"
                     /pseudo
     CDS             complement(39129..39641)
                     /locus_tag="ACHGNS_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(39767..40468)
                     /locus_tag="ACHGNS_000594"
     CDS             complement(39767..40468)
                     /locus_tag="ACHGNS_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN6936574.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(40576..42019)
                     /locus_tag="ACHGNS_000595"
                     /pseudo
     CDS             complement(40576..42019)
                     /locus_tag="ACHGNS_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            complement(42033..43898)
                     /locus_tag="ACHGNS_000596"
     CDS             complement(42033..43898)
                     /locus_tag="ACHGNS_000596"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFN6936575.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(44019..44869)
                     /gene="licT"
                     /locus_tag="ACHGNS_000597"
                     /pseudo
     CDS             complement(44019..44869)
                     /gene="licT"
                     /locus_tag="ACHGNS_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            complement(45179..45892)
                     /locus_tag="ACHGNS_000598"
     CDS             complement(45179..45892)
                     /locus_tag="ACHGNS_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFN6936576.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(45960..46286)
                     /locus_tag="ACHGNS_000599"
     CDS             complement(45960..46286)
                     /locus_tag="ACHGNS_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFN6936577.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(46409..46636)
                     /locus_tag="ACHGNS_000600"
     CDS             complement(46409..46636)
                     /locus_tag="ACHGNS_000600"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFN6936578.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(46958..48211)
                     /locus_tag="ACHGNS_000601"
     CDS             complement(46958..48211)
                     /locus_tag="ACHGNS_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFN6936579.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            48272..49012
                     /locus_tag="ACHGNS_000602"
     CDS             48272..49012
                     /locus_tag="ACHGNS_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFN6936580.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(49098..49856)
                     /locus_tag="ACHGNS_000603"
     CDS             complement(49098..49856)
                     /locus_tag="ACHGNS_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFN6936581.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(49853..50317)
                     /locus_tag="ACHGNS_000604"
                     /pseudo
     CDS             complement(49853..50317)
                     /locus_tag="ACHGNS_000604"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            complement(50336..50833)
                     /locus_tag="ACHGNS_000605"
     CDS             complement(50336..50833)
                     /locus_tag="ACHGNS_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFN6936582.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            complement(50901..54068)
                     /locus_tag="ACHGNS_000606"
     CDS             complement(50901..54068)
                     /locus_tag="ACHGNS_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFN6936583.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(54292..55176)
                     /locus_tag="ACHGNS_000607"
     CDS             complement(54292..55176)
                     /locus_tag="ACHGNS_000607"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFN6936584.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(55271..55708)
                     /locus_tag="ACHGNS_000608"
     CDS             complement(55271..55708)
                     /locus_tag="ACHGNS_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936585.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(55925..56836)
                     /locus_tag="ACHGNS_000609"
     CDS             complement(55925..56836)
                     /locus_tag="ACHGNS_000609"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFN6936586.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(57293..57721)
                     /locus_tag="ACHGNS_000610"
     CDS             complement(57293..57721)
                     /locus_tag="ACHGNS_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936587.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(57749..58387)
                     /locus_tag="ACHGNS_000611"
     CDS             complement(57749..58387)
                     /locus_tag="ACHGNS_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6936588.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(58391..60427)
                     /locus_tag="ACHGNS_000612"
     CDS             complement(58391..60427)
                     /locus_tag="ACHGNS_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFN6936589.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            60553..61218
                     /locus_tag="ACHGNS_000613"
     CDS             60553..61218
                     /locus_tag="ACHGNS_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6936590.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
BASE COUNT        18190 a        12966 c        10620 g        20714 t
ORIGIN      
        1 tttttaattt attgcccgat aatatataga aataaggtct agttactgtt ttttttaaat
       61 gattaataaa taacaacttc tttctatgaa attgcaaagc attcatcttc ttttccgcta
      121 acgatatatc cgtcagtgct tggtacactt tactaaaaag tatgagattg ccgatatctg
      181 gataggtgtg ctatactaca gaaaaagata tacaatctgc caccgggtag gataaagagg
      241 atgatcattc cattaggtct ttgaagggat gattttcctc tttttgtgta tttttttata
      301 gttaaggagg aatgtaagta tggcatggat ggaattaatc atagcaggaa ttttggaagt
      361 attctggtca acgatgatga aatggagtga tggtttttct aaaatcaatt attcttttta
      421 cacagtgatt ggcatgattg ctagttttta ctttttgtca aaggcaatca aaagtttgcc
      481 gatgagtctc tcttatccta tctggacagg gattggcgca gttggttcgg tcataattgg
      541 tgtggtcttg tttcatgata agctgaatct ttccacttgg ttctttgtgg ggatgctact
      601 catcagtatc attggcatca agattactag cggccactag acacttggaa taagtactga
      661 tcagaaaaaa taagcctttt tatttaatat tcaatttttt tatgaaaaaa taagaaataa
      721 gtccaatgac ctagcgactt ctccactcat caggtgtatg cttgtactat ctatagaaaa
      781 ggagtgaata tttttgaaaa gtctttcggt ccaagaatta tccaagaact ttggtcacaa
      841 acaagccctc agcgatgtca gttttagctt atcccctggc aaaatcgttg gattagtagg
      901 tcctaatggc gctggaaaaa caacgatcat gaaagctatt ttaggactga tccattactc
      961 agatggaaca atctgtattg gtgaaaatga gatttctccc tctagtcata aagggttgga
     1021 acaagtagga gcattgatcg aatacccggg tatctatcca ttcttaacag gctatgatca
     1081 tctgaagtta ttctctgaaa ccaacgatgt atctgctatc gacactatcg tgaatcagct
     1141 caagatggaa aagtacattc acaaaaaagc gaaatcctac tcgttgggaa tgaaacaaaa
     1201 attgggaatt gctcttgcct tactgaacca tccgtctatc gttattttag atgagccgat
     1261 gaacggtttg gatccacaag caacaagaga tgtgcgaaaa atcattaccg acttggctac
     1321 tcagggcacc tctttcttga tctccagtca cttactcagc gagcttgaaa aaatggtcga
     1381 tgagctgatc ctgatcaatc aaggaaagat catcaaacaa tgtacaatgg ctgaattgaa
     1441 ccaaacagcg caagatttca tcgttatctc tacaacaaat gatctagctg ctcgaacgat
     1501 cttgacagaa gctggatata ctttggaaga tcctgataaa atcaagcttt taaaagaaaa
     1561 cgaagaatta ttaggaaccg tgatccagct attgtctgca caaaatatcc atgtgacaga
     1621 cgtccagcat ctgacgaatg atttggaatc ttctctattg gaaattctgg attcagtaaa
     1681 gggggaagaa gaaaaatgac attgcttgta aaacaagaat tatttaaatt gatcaagaaa
     1741 aagtcaactg ctgttttatc tgttctatta gttgttttgc tcataggaac agcacttcta
     1801 gccaaaaaat acacgacgat cattgatcca gttgaaatga ctgctcagct ttttagtgcc
     1861 acatcttgga ttgtctttat catgattgca gctgcaagca cgatcatttc gatggaagca
     1921 caatatggta cattgaaaaa tctcctttat cgaaagtatt ctcgaggaga aatattagta
     1981 agcaaatgga tcacattagt tatttattct gtttatttat accttttagc tattattgtc
     2041 actgtgttga tgaaactgat tcttttccct tcgatttcct tcacggagaa ggtttctact
     2101 ggtcaaacac tgatccaaag cttgttcact tatactttgg gtagttatat cggtttatgg
     2161 ctgattttaa gtttagtgtt gatgattgct tgctttatca atagttccgg cgcatccatc
     2221 tcagcaggaa tcgtctttta ttttgcttcg tcaatcatat ctggtatcct tattgcactc
     2281 atccaaaaat gggagtggat caaatggaat ccgatcagta tgttgaatct gcaaaaccaa
     2341 attggaaacg aagagatcat gaagcaattg actcatcttt ctactaatca aatgctattc
     2401 ggtaatttag cttacatcgt tttgttctta gcattaggat atctcgtatt taagcgaaaa
     2461 aatgtatgat gactcatcaa aaaagaggct gtgaaagaga cgttcagcct tattgccgat
     2521 tggctgtctc ttaaacaccg tttattctga aaaaggagct gtgacaacat ttgtgtcaca
     2581 gctcctttcc tagtatgttt ttgaataaac gacaggctca caacctctct ttatacttct
     2641 tttatacttc tatacgtgaa gcatcccagt ggcaaataaa accattgcaa tcactccgac
     2701 catcgtcaca cctataagta aaatcttatc taagtggata tgttgtacag gctcgttttg
     2761 ctccttacgt gctttgaggt aaaaataaat gcctggcaag aaaagaacaa gaacaagcat
     2821 tacttcatta aaggctacga ctagcatagc ccatacttga aaggcacttg ctaaaagccc
     2881 tactaaaaat tggacccatt ctttccgctg ataagaaagt ttcatttggt acaatcctaa
     2941 gaaaagccac gtgaagaaaa tagctgctgt accaagtgtg taagcaaagt tataagcact
     3001 gtcagtgaat aataatgaaa gtaaaaagac attcgtacat aacgtcgtca atacgatagc
     3061 ataagttggt gccccatttt tgttgagttt tccccatgct ttaggcaaaa gtccgtcttc
     3121 agccatgttt cccattgttt caatcggtaa gatcgtccat gagagccaaa caccaactaa
     3181 agaaatgatc aaaccgccgt tgatcacgac tgctccccat ttaccaacca tcatttcaaa
     3241 cacatagccc atagcaggct gcgccttgat cgtttgcagc tgattcatcg tcattgtccc
     3301 atatggaagc atggaaacca agacgtaaat aataatcaag ccgaatatcc ctagaatcgt
     3361 tgctttccca acatctgatt gtttttttgc tcgtgaacct acgacagagg ctccttcaat
     3421 accaacaaat acccaaagca tcaccatgat gcaactcaat acttgactgg aaaacgggat
     3481 cggctgtcca ttcgcattca atataatgtc gctagaaaag ttatcaatga attgttggat
     3541 cgaaaacgtg ctgatagcta ctacaataaa taaaaataat ggcactaatt tacagaatgt
     3601 gacgatagca ttgacgaaac tagcattttc gactccccga ttcactagcc atgcatatcc
     3661 ccataaaaag actgtactca tcacgatcga tgccagattt tgcccacctt caaatactgg
     3721 gaagaaatat cctaatgcgc tcatcaacaa tgttgcaaaa gctacgttgc ctaaccagca
     3781 tgtgagccag taaccccaac cgctgataaa accagcgaaa cggccaaaac cttcttctgc
     3841 gtaactaaag atcccgcctt ttaaatctgg acgtttttta ttgatattat tgatcgacaa
     3901 ggagagcatc aacattgctg ttcctacgag tacccaactg aaaatagctg ggccgccagc
     3961 cgatccggag atgtccgtca taataccaaa tacgccacct ccaatcgctg aaccaattac
     4021 taaggcagca agtggaatta aaccaattcc ttttttctgt tgctctgtat ccatgtaagc
     4081 atcctccaaa ataaaaccgc ttttatcaaa aagtataaca aacctttttt atttttactt
     4141 aattaataaa aactttaata gaaatataat tctaccggct tttttctcat tatacaagca
     4201 aagcgcctag tccctttcca aattacacca ccaaaaaact taaaaaagat gctatactaa
     4261 aaggaaagaa ttggaggatg atccatgaac attttatata tatcgatttc aggaaatacg
     4321 cgtgcctttg ccaaacatct agcagaatat gctgaaaaaa tgcatgcaga agatcctgtc
     4381 aatcctgaag ttactttaaa agaaatccat gaaaatagtg attttacgaa agaaacagaa
     4441 ccatttttta ctttcgttcc tacttattta gacggcggaa atggtttaga taacggtgat
     4501 acagaaatct tgaccgaaac gatgcgtgaa tatctggaac atgaagacaa tcataaactt
     4561 tgtcttggtg tagtcggtag cggaaataaa aacttcaata atcagtattg tctaacagcc
     4621 aaacaatatg cacaaacatt tggctttcct ttcttggcag attatgaatt acggggaaca
     4681 ccatctgatg tgacacgaat ttatcagatt ttagcagaaa acaataaata ataaaaaaga
     4741 agaagctgaa aaatagattt cagtttcttc tttcgtttct attgtttcta tcatattaag
     4801 ctttgacatc catcacaggt gcttttgctt ttgctttacc tgttttttcc aagacaaagt
     4861 tttttacacg atccatattg gttaccaaga cgatcatgga tgtttctttg cctgctgctt
     4921 tgatcgcttc taagtcagct tctgctacaa gcgtatcagg tgtcacttgc tgcccttctg
     4981 ttactttcac atcaaatggc gtaccgccta agtcaactgt gttgattccc atatgaagca
     5041 agatttctaa accatttggc gttgtgatcc caatagcgtg tttcgttggg aagacagagc
     5101 tgactacccc agtaactgga gaatagatat gtccgttttc tggttctaca aagtagcctt
     5161 cacccatcat cttttgtgca aaaactggat cacttgcttt atctaaagaa acaactgttc
     5221 cgtcagcaac tgatgagagg atagtcacac tttcagctga agtttgaggt actacttttt
     5281 caggcacttc atttactgtg gctgtttcag tcgctaattc ttctttttct aaacggtctt
     5341 taggaatacc gaagaagaat gtagcaacga agccaccagc ataagctgcc aataatccaa
     5401 ggatgtagcc taaccattta ccgtcagaga tcaatgggat caaagcggct cctgaaggtc
     5461 caatagcgat agccccgaca tttccgatca ttccgatcac tgccccgccg atacctccac
     5521 cgatacaagc agtaataaat gggcgtccta gaggtaatgt tactccgtaa atcaatggtt
     5581 cgccgattcc taggatcccg actggtaaag ctcctttgat catttctgtt aattctttat
     5641 cttttttgca gcgaatccat aatgccaatg ctgcacccac ttgtcctgca ccagccatcg
     5701 caagaattgg aagcaatagc gtacgaccag tttcatcaat catttgcaca tggattggag
     5761 tcaaaatttg atgcagtccg aacataacca tcggcaagaa taatgtgcct agaacgaagc
     5821 cggcaaatgc tccaccaacg ccaagtaccc agttgatccc accgaccatg ccatcagaaa
     5881 tgaaacctgc taaaggcatg atgaagaaaa tttcaatcag accgatggca atcaatgaaa
     5941 gagttggtgt aacgatgata tctaccgcat caggtactac tttatgcagt tttttctcaa
     6001 cgattgacaa tagccaaaca gcgaagatga cacctaggat cccaccttgt ccagctgcaa
     6061 gagcttcacc agtaaataag tttttcagtg gtgcttccgg attcatccct gtcagcatca
     6121 caacggcccc gataactcca ccaagagttg gtgttgcacc aaaaacttgt gcagcattga
     6181 tacctgtata gataactagg tagctaaaca tcccgttttt caagatgttc atcacatcga
     6241 tatattgttg ccatgttgct gcatcaagtg ttccggctgt gactaagttt gctaaaactg
     6301 cggcaatccc tgccacgatc ccagtaccga caaatgcagg aatcattggc acaaagatat
     6361 tagaaatgtc ttttaatacc gatttgattt tagatggttt ttgtttcgat ttttgtgccg
     6421 ctttcatttc ttgtgcccgt tcgtttacac ggtcttttcc agatgctgat gaatttgctg
     6481 tagctgtact tacagaagaa agtttttctc ctaatttcac gcctgccaaa tcaaccattt
     6541 gctgtgctac tttgttcact ttcccaggtc cgatgatgac ttgaagctgt tcatcttcta
     6601 ctacaccaag cactcctgga atcgctttta attcagagac atttacaaga tcatcattga
     6661 tgatcttcat acgcacacga gtcatgcagt ggacaatgct ttctacattg cccatgccgc
     6721 ctgccccttt ataaatttgt tttgccagtt cttccaaagt cagttcgttt tttgccatgg
     6781 tcttttcccc ctcataaggt cccttttaca aatccgtttg cttcagctaa ttttgcttga
     6841 gcttcctctt tatcactatt tgtcaaaatc atgacgatag ccagcttaac attttgatct
     6901 gcttcttcaa agtacttttc agctgtttcg taatcagctt ctgtcgcttg catgatgatt
     6961 cgttttgagc gttcgattag tttttcattt gttggtttga catcgaccat caagttattg
     7021 aatactttac ctataccaat catactgata gtagaaatca tattcaagat aagtttttgt
     7081 gctgttcctg acttcaatcg agtagagcct gtcaaaaatt ctggacctgc atcgacttcg
     7141 attgggaaat ccgcgtgttt actgatttcg gcttgtttat tgcaagaaat cgttcctgtg
     7201 acagcaccga tcgattttgc gtaatctaat ccaccgatca catatggtgt acgaccgcta
     7261 gctgcgatac caataactat atcatttttt gtcaaattca gcttgattag atcttcttgc
     7321 cctagctcat aagaatcttc ggccccttcg acagctacag tcattgcttc ttctccacca
     7381 gcaattagac ctatgaccat ttccggttct acaccgaatg tcggtacgca ttcagcagca
     7441 tctaacacac ctagtcttcc acttgttccg gctcccatat aaatcagacg tccacctttt
     7501 ttaaaggact caatcgtgtt agcaatcact ggttcgatac ttgtcagttc tttttcaacc
     7561 gcagcggcca ctttctgatc ttctttgttc attagttgaa cggcttgaga aatactcatc
     7621 tcatctaatc caaaagtttc ttggtttctg cgttccgttg ttaggttctc taaattgatt
     7681 ttgctcattt ttatcttcct tcttttctta taaattgata gttcatacca gctttgattt
     7741 ggttgatcaa tggtagctct tctttgataa ctttagctac tacattcact ttttcatccg
     7801 ctggcaaatc atatttggtt aattggatct cacccatata gcgcaaatac ttttcgttat
     7861 ctaaagtaat agaccctttc atacgcgtag ctgtatttct tgctgggata ggcggaatct
     7921 ctcgaaaacg tgcatctacg cttcgtatga catctcttgc atcgtcttgc cgattcgtat
     7981 gcttccctaa tatatattcg taaaattctt catctacagg ttccgtatgg agcaagactg
     8041 tctgttcttt ttggaaagaa gcaaattgct cctgaacgtt ttctgacaga cctgcatctc
     8101 cgatgtaaac aatatccgtg ttacttgcta atagatctag agcgcctgca agaggatgga
     8161 tccctcgatg ttcttccaaa gttggcaatc cttgatatag tgggcctctc aactctgtat
     8221 ctcctggtac aaaaccttgg acaaggagac cttgaatttt gagccattga ttttttttct
     8281 gataccattc tttgtccagc cctgtttcgg gacgtggata ataattatgc caagcttcaa
     8341 tctgtgagaa atcagcttct gcttctttca attcatcgat atctttaggc gtgatcgtgc
     8401 tagcattcaa actgattttc aattgatgtg accagtcggc aatctgctga ttggaaatat
     8461 gataatccat tcttaaacct gttacaccaa ttgctttcaa aggcgttaga tcttctaatg
     8521 aaaaacccgc tcgttccaat gcctctcctg aaatatcgac catcaattct agtttattct
     8581 ttttcgcttc gcttcctagt tcgatcaatc gcttttgata tgccgaaaca tcatcttctg
     8641 gtatatgcat ggaagtgaaa ataccgacaa agccattgga tgccatttgg cggatatact
     8701 gtttcttctc ttctgacaaa gaatcgttca taaatattga aaagccaaac atattatccc
     8761 tctttttctg tatccgtctt catttctatg actagagtat atatctaaag aaacaaaatt
     8821 tcaatacttt tattaaaata attaaatttc atttctaaag gttgagttta tattatcaaa
     8881 agaattgtca taacctaaaa aaggcactgc taagcaaaag cagtgccttg aaatatttta
     8941 tttaaataac acaaaaacat aattactcat aagaaaccaa ttctgtatta tctaccatga
     9001 atgtaaagaa cccctcatta ggcatcggat gaaaataagc attgatcact cttatcaagg
     9061 cattatggca aactaacaga tagacatttt cttcgtcttc gatgcattct ttcaataaag
     9121 gaacgatccg tgcatacaca tctaagactg actccccatt tgggaatcgt acagcgaacg
     9181 ctaaacgtgc tttttgaaag ttctcatctt tgcttggcag tccatcataa tccccaaaat
     9241 ccatttctac caatcgttca tcaacggtca gcggcaagct gagacgatca gcaactgctt
     9301 gtgctgtatc tcttgctcgt tgaagagggg aatggatgat tttcgtgatt ggaacaggca
     9361 aatctactac cttttctgct agtttctgtg cctgcatctg tcctttttca gtcagcggca
     9421 catctgcccg tccgcaaact aatccttgtg cgttccaagt tgtttctccg tgtctcgtta
     9481 cataaagcat gtgattctct ccttcgttgg caaagttccc cttaaccata ccttgttgtt
     9541 cttgagatta caagggttag attctctcaa gagaaataat ctcagtagat actttatcca
     9601 ggaaggtttg atcatgttcc actagtaaca tggcaggttt cacgttcaag atcagttgtt
     9661 ctaattgttc ttgattgaag acatccaaat aattcaatgg ttcatcccat gtatatagtt
     9721 cagctggctg tgataaagat ttagccaatt ccactttttt ccgttggccc atactcatct
     9781 gctcgatctt gttatgaaaa acatctcttt ccatcccaag ctttcggagg ttgttcaaaa
     9841 atgcttggta gtctacttgg tttttctctg caaattccgc caacgttcct cgattgtctt
     9901 cataattttg gcttgcataa ctaatgctca gatgttttgg ctggtatttt tctcctataa
     9961 ccttgccgtt gaatgccccc agaagataat ggataatgga tgactttcct gcaccgttag
    10021 gacctgaaat cgccacccgc tgatgaggct cgattgtaaa atgaattggc tcgaataaca
    10081 gattttcata ccctaattga agatcttcta cgcttaaaag tcgcttatgg tgagacgctt
    10141 ggctattcat cgtcaaagaa tcgatatatt cgatatcttt taatagtttt tctttctcgc
    10201 tgatctgggt ctccatccga ttcacgattg ctttggatcg tttcatcgtc cgtgcagcat
    10261 cagcacccac tgctccttta ttcactcgtc tagattcagt atcgatgaat ccgacttgtt
    10321 tctttgtttt atctccttct cgggaacgag accattcggc tttttcagct gctgttttct
    10381 ttagcctact gacttctttt ttcaattttt cattttgagc catttcaaat tcatcacgaa
    10441 gttttttctg ttcttcatag atagagaaat tcccttgata aagttccagt tgactttttt
    10501 cgattgctaa aacatggtcc actacttcat cgataaatcc ccggtcatgg ctgaccacga
    10561 tgaagccttg ttttttcttt ttcaaataag ccgctacttg ttttctacca gagatatcca
    10621 aatgattcgt tggttcatcg attaacggga aatgagtgtc atccacaaat aaaagtgcca
    10681 gtaggacttt tgtcttctct ccccccgata gtgtgctgaa ttctctccat aagatttcag
    10741 gatctgtttg catcaattgg agctctcttt cgatttccca tatctcaaaa tccgtaatat
    10801 catttaacac gtaataggtt aaacgttctt tatcttttgt ctgttgcggg aaataggcaa
    10861 attcttgctg atggattact tgcccttgat aaggtagttt gttttgtaga atattcagta
    10921 aagttgtctt tcctcgaccg tttcgtccga taagtcctag tttccattgt gtgtcaaaat
    10981 ttagatttgc ttgttcaaat aataatgtgc cttggctgtc gtagccgaat gtcagatttt
    11041 ttatttcgat tttagacatt ttccatcacc tcgtaaaaaa agaccagtcc tattttcaga
    11101 actggctaaa gaggatgcaa gggttggata cacacaaaaa gacccataca tcgaaaaaac
    11161 caagccaatc ccgaaaatct gcacactgct tccttgtctc cacaaggtac agtctgaatg
    11221 cagctttcat caatcgactt aatttttcaa tgcacggatc cctcactttg tcctatattt
    11281 gaaattacga tagcataaag aaaatgattt tgtcaactac ttcgggagga tggtgaaaaa
    11341 tctgctaagt gtctgtccta agatttttgt tttttctaat gtttgcctgt gttccattgc
    11401 ttcttctaaa cctattggac ccaaatgaat acttaagaca attgggagaa aatcagaaag
    11461 tccttctagc tcttggtctc tacttccgca aatagcgatt attggttttc gatatttttt
    11521 tgcaagcact gctacaccgt atggaacttt tccgtggatc gtttgctgat cgatccgccc
    11581 ttctcctgta atgatgacat ctgcttgttc gatctttttt tccaatccag tgatttggct
    11641 gactaaatcg aaaccagaaa ccatcttcgc tcccaacaaa aaaagggctc caccaatacc
    11701 gcctgctgct ccacttcctg gaatatcatc taaatccacc tcccagaaat cttttatttg
    11761 ctgagcaatc tttcctgctt gtttatctaa gaaagctagc gtcgtatcta ttcctccttt
    11821 ttgtcttcca aattgccaag cagctccttg ttgacctgta tatggattgg taacatctgc
    11881 agcgataatc aattttactt tagaaacaag aacgtttctt cgatcaaaat gaagtttttt
    11941 agtagataat aaaggatttc cttctgtgta cccttctaaa gaagcaccca aggattggag
    12001 taatcctaga ccaccatcgc tacacccgct tcctcctaaa gtgacaataa tttgttctac
    12061 ttcttgaagc agtgcatctt taataagttc tcctaaggca taactggaag caagtcggat
    12121 agtttgatcc gttggtttat caagtagatc taaccccaat gcttgtgcag attcgatgac
    12181 tgcggtgttt tttccttgcc aagttgtcaa taaatagctg atttttcgct gacggaataa
    12241 gagatccatc ccatcaatgg tacgccatgt tcccctgagc gcgtaataga ttgcttctgc
    12301 agtattttct ccgccatctg caatcggcac attgattttt cttcctttac gatcttccca
    12361 gccatttaga actgcctgat ttagttcaaa tgaagaagca cttcctttaa atgaatcaat
    12421 tgccgccaaa atatccatct gtatccctca ctttcttctt aagtaagaca ctttttttca
    12481 tttttttcaa acaaaacaat gctattttct tccatttttc tatatactaa aagggaactc
    12541 attgaataaa ggagagaaaa aacatgaaaa tcgcaatagc tggggccgga gcaatgggca
    12601 gccgaatcgg attgatgcta catcaaagcg gaaatgaggt cctcttgatc gatcgttggc
    12661 ctgctcacat tgaagccatt cgtacaaatg ggttgattgc tgactttaac ggtaaagaag
    12721 tggtcgcaaa actgccaatc tattcaccag aagaaatcat cgaaagcaac gaacacgtgg
    12781 acttgatcgt agcattaaca aaagccaatc aattagatga tatgttttgt tcgatccaat
    12841 ccattattac tgacaatact tatgttttgt gtttactaaa tggattaggt catgaagatg
    12901 tactggaaaa atatgtgcca aagaaaaata tcttatttgg gatcacgatg tggacagctg
    12961 gtctagctgg tcccggaaaa gtcacattgt taggtgatgg agaaatcgaa ctagagaact
    13021 tggaaccaga aggcgaagca tttacaaaaa aagttgtaga agtctttcaa gaagcaaatt
    13081 taaatccgat ctatagccat aacgttcgtt attccatttg gcgaaaagct tgtgtaaatg
    13141 gaacattgaa tggtttatgc acgattttgg attgtaatat tgctgaacta ggcgcgcaaa
    13201 aagcggcaga gagcatggtt cgaacaatcg tatctgaatt tgcttctatc gcagcaaaag
    13261 aaggaatcat actagatcaa gaagaagtgt accaacatat tgcttctact tatgatcctg
    13321 acaacatcgg gttgcattac ccatctatgt accaagacct gatcaaaaac catcgtttga
    13381 ctgaaatcga ctacatcaat ggtgccattt ggcgaaaagg acaaaaatat gatattgcta
    13441 caccatattg tgcctttttg acccaattag tccatgcaaa agaaggaatc cttggcgcag
    13501 aataaaaggc tctctgatga gaatcagctg aatcagcagc tggttctctt tttttaagcc
    13561 caataaaaga tcaaaaagaa aacacaactc tcatgatgcc ataagaattg tgcttgtttg
    13621 tttcagtaca taatctcttc aaaatgtagg ttttttattt gttttcaaca gaggagaggt
    13681 catattcgta ttgctcataa atagtggaat cgttagtgat aaatcgtttt gcccaatttt
    13741 ctgcatcata aaattccaat agctctgata ggattggctt aaatggtgta tgcagaaatt
    13801 ccggtaattc atctaaatca cgttctcgaa gcaaaaaaat attttcagga cgataaaaat
    13861 gataatgagc aatcgcttga ttatccgtca ctagtttctt ttgaaaatac atggattcaa
    13921 atgtccgcaa agtcactcct tgctgaccct cccgcaacag ttccagaatc ccgcgcgatt
    13981 cccctgtttt tttcaggtac tcttcataag taagatatcc aggttgttca ttccctcgat
    14041 gtggaagaat gaaatagttg gtcgtaacat ctctttttat aaattcctgc ttatagcttt
    14101 ctgctcgttc ttttctccca ttattcgttg caccaaaaaa tagatccgtc tttggttcgc
    14161 tagaagaaaa attcatatgt ttcgaataaa atgaactatt atgtttcagc ccaagttctt
    14221 ttgcttccaa aaaatcaaaa tgataaatct catcgattgc cggttcatct tgtgcctgct
    14281 tcagcatcgc ctcgtgttct gctttgaagt gattccaaaa aaataacaca atttttgcat
    14341 tggtaaatgg gcgcacgcgt cgagccatct ccatatcaaa tccataatct aaaaaaacga
    14401 tcagctgata atttgccaac tgttgtctag ctgatttaat gaattccgac tgtagtaaat
    14461 cttttccgga ccaaacatct aaagagcgga tggaaggcaa aaaataaagt gatttatttt
    14521 ttgcaactag cagtacgtca tttttgttca tcttttccgc ctcactttct ctgtcacttt
    14581 ttcctatgat agcctgttta aatagtaaaa agccagtaaa acgtttccta tatttgtatg
    14641 aaaatttaac atttgtatga aaatatgatg aaataaatca ttcttctatt aaaattcgcc
    14701 taaaagtttt ctgaatagtt gtaattgtct gaaaatttgt tatcattagt cattgttaaa
    14761 tattgctatt tattcttaat ataaattttg attgtgaatt taaaagaaag aggtcattta
    14821 tggaagataa aaaattgaaa aaacaactta gctccagaca tatcaccatg ttagctttag
    14881 gtggagcaat cggtgcaggt ctatttaaag gaagtggcga agcgattggg atcgccggcc
    14941 cttctgtatt gattgctttt ttgattggtg ggctgatttt atatatcgtg atgaaaggtc
    15001 tgggaaaaat cgttttaagc ggtggcgaca cgcatcacgg attatctggt ttgatacgcc
    15061 cttatctagg tgctcattct gctgatttta ctgattgggt ctactggtct atgtggatta
    15121 tcaatatcat cgctgaagct gttgcagctg cttccttttt gcagttgtgg ttcccgcaca
    15181 ttccagcctg ggtatttgtt tttatgttgg ctgttttaac tacgatcatc aatctttatt
    15241 cggtacggtt attcgcagaa acagaatatt ggctggcttt tgctaaaatc agtgtcatta
    15301 tcttactgat catttttgct acttatcttg tcggtcagca aatgttgggc acaggcgttt
    15361 tccctaccct tcagcaattg actgaccatg gtgggtttac tcctcatggt atgaagggaa
    15421 tcatcagttc tttgcttgta gttatctatt cttacggcgg ttctgaactg attgctatca
    15481 cggtaagcga agcagatgat cctaaacgtg cgattcctaa agcaatcaaa ggtgtcatgg
    15541 gaagaatcat ttccttttac atcattcctt tgtttttgct tttgattctt tttccttgga
    15601 atactttagc aggcacgaat gtcagtcctt ttgtgatggt atttgaaaaa atgaatatcc
    15661 catttgcagc ggatatcgtc aattttgtga ttgtccttgc tttgttctca tctatcaatt
    15721 cgggtgtgta tgcatcttca agaattttgt tcttccgttt gaaggatcga aaaggttcct
    15781 ctagaaaact agctgtttta aacaagcatc aagtaccgca acgtgctgtt ttattttgta
    15841 ccagtgttct ttatcttggt gtagctcttt cttattttgt cggtgacaaa ttatttggct
    15901 atctggcagg ttccctttcg tatacagtat tattgatctg gatcattatc agtgcggcat
    15961 cctttgtact ggcactaaga aaaggaacat tatgggataa aggaatcagc ttttttgcat
    16021 tagctgtctt aggattgatt ttccttggta tcctgttcac gaactccatt ggggtaactg
    16081 tattgactgg cctgctttac ctgttcattt atttcagtta tcaaaaaaag aatgatgcgt
    16141 tcgtattgac gaatgaatga aatcgataga aaattttatt ttatttatgt ggaatagatg
    16201 gagctattga agaaaggagt tgtgacaaaa gtcttgttgc aactcctttt tccgaataaa
    16261 cagtctcatg acctcttggt aaacggtgtt caaaaaccct ctgtgcaaaa taattgattg
    16321 ttctccatgc tcatgacatg atagaagaaa ggaatgaaaa aaaggagttt gttttaaatg
    16381 aaaaagtatt ggaagttttt gttgatgatc ggtgtttcaa cggtagttat gtttggtatg
    16441 atgtacttga atgtctatgc gctcgatcat atcttcttca gtcgtacacg agtattcatg
    16501 gcgttgatga tgggtgcagt catggcagtc atcatgctgt tgttcatgtg gaaaatgtat
    16561 gaaaacaaaa agataaatgt tatgatctta gccgctagtc ttcttgtttt tgccggttcc
    16621 ttgtttatgg tacgaagcca aactactgtc aacgacacag cttggatgaa ggccatgatt
    16681 cctcatcatt ctatcgctat cctggcaagt aaacgagctg atttaaaaga tcctgaagta
    16741 aaaaaattag cggaagaaat cattcaggca caagaagaag agatcgacaa aatgaaaaaa
    16801 atgatcaaag aaatggaatg accattatcc gtccaaataa aaaaagagga tgttccagaa
    16861 gttatcagct tcaagaaata agaagaagct acttctggaa cgccgtgaat aaggggttgt
    16921 gaaaaagctg ccctgcatca agtaataaga acagccaaac aaaaatagct tctcctattt
    16981 ttcgtttggt taggcttatt accgcagatg caagcttttg aacaccgttt attaagaggt
    17041 tgtaaaatca gcgttctgac ctgagtatta gagagacaaa tggaaaaata gttcctcata
    17101 ttttgccatt ttttgagcta ataccgaggg tcagctgatt gaacaccgtt tatcgagagg
    17161 ttgtgaaaaa gtcgcttagt ttcgcgtatt aagaaaaatt ttgtgaaatg gctttccaca
    17221 tttttgtata aatttggctt aataccgaga aactggcttt tgaataccgt ttattcgtaa
    17281 aagggattgt gacaaaagtc ttgtcacaat ccctttttat gtttttattg ttctaattgc
    17341 gctgtcttgc ttactgcttc ttgcggtttc acaaattttt ttgagattcc ccaacctgtg
    17401 aaggcactga ccatcgaaaa cacaggagaa agcaaactga aaaatacgaa tggcaagtaa
    17461 gtgagtgtag gaataccaaa tgtacttgct acgaaacttc cggcaattcc ccaaggcact
    17521 aagtagttga ttactgttcc accatcttct agcgttctac ctaatacagt tggatcaaat
    17581 cctgcttttt cataagtttc tttaaatgca ttaccaggca gaataacaga taggaattgt
    17641 tcaccaataa aaatatttac tccgatactt gtcatcaatg ttgtaaaaat caaatttggt
    17701 gtgttcgtca tctttcttga tactcgttcc atcacggttg agatcaagcc aaattcgatc
    17761 aacaatccac ctaatgacaa agttagaata atcaatgaca cagtaggcat catactttcg
    17821 attcctccgc ggctaagcag tgaatcgatt tgttggtttc cagtatttga aacaaaccct
    17881 gattcgatga ccccagccaa cttcgttaag gaagtttgtg ggttttgaat aaaaatcatt
    17941 cctactgcta cagcgatatt caacaaaatc gttaaaattg ccggcacctt tttccatgca
    18001 cacagtaaca ttaaaaataa aggaatcagt gaccagattg aaatagtaaa gtttgcttct
    18061 agaacatcag tcacttcacg aatagatgaa agacttgcgg ctttacttgt gtgtcctaaa
    18121 atcatgaata aaaagaaaga aacaacaaat gccgggacag ttgaccacat catatgcttg
    18181 atgtgtttga acaaatcggc atcaacaata gctgctgcta gatttgttga ttcggataat
    18241 ggagacatct tatctccaaa aacggcacct gaaacaatag ctccaacaac cagcgctgga
    18301 tttaagccta gtgttgtccc aataccgaaa aatgcaatcc caatcgttga cataacagta
    18361 aaggcactcc ctacagcgct acctacgatt gcacacacaa caaatacaga agggacaaac
    18421 cactggacac tgatcaattt gaacccaatg accattagag ttggaatgat ccctgcttgt
    18481 atccatacag caatcaatga accgactaaa ataaaaataa agattgggat gattcctggt
    18541 ttgatgccat tgatgattcc ttcgtgcagc caatctgtcg ggtatctttt tatcactgca
    18601 tacaacatga ccattccgat cgctgctaag atcgtgatat taggtgagac ctttaatccg
    18661 acaacgccaa tcgcaatgcc taaaacaata accattaata caagtgttgc ttctaaaaaa
    18721 cttggtgttc ctttttttcc catgataatc tctccttttt tagttgttct tatgttctaa
    18781 aaaagttaag atatcggatt actccgccgc aattcatccg cgattcctcc tttataaaaa
    18841 taaaaagacc tgttttaaac taaggacgaa tgactccgcg gtaccacctt aattaaaaac
    18901 agatctgttt ttcactttca aacttttatt caaatgtatg gactttccat gtaattcgtc
    18961 aattaggctt gatcggttca cagcgaccac cgactttctg aacaaaacac ctaaccgcta
    19021 ctgcgtgaaa agttgacttg tacgaagttt accttccata agatcatttg tcaatgaaaa
    19081 agaaaatatt ttttcatttt tctgaaaatt atttttataa ctttcacaca caagtttatt
    19141 ttttactaga aagtagcgcc ctcttgtttt tttcttgcta ctatttcttg atgttcttgc
    19201 ttgattcttt ctaatagctt tggctctttc aacagatcaa gagctgtatt ggcgagtagt
    19261 tctgctgcaa tcgcaataga agcaaggcct ttgtcacttt tagcagcagc tttgaattct
    19321 tctgtgtgtc cggcaatata tgtctcagag attgctaccg taggctggat tgttggtatg
    19381 acttggctga catttcctac atcagaagaa ccgagacttg ttctttgttc cgtttctatt
    19441 tcttcatctg gaacacctgc ttcttttgca tgctggaaga ataagtcatc aaaagaagga
    19501 gtaacgatga catcatcaac tgcattttgg aacaatccaa actcaaaagt cgtgcccgta
    19561 cttatcgcag cacctcgtac gatattttcc actttttcat atactttatc taatgtttgc
    19621 cgatttcctg cacgtaaata gaaacgtcca gcagcgtaat ctggaatgac gttggcagct
    19681 actcctccgt ctgtaatgat cccatggata ttgacatctt ttggcaattg caagcgtaga
    19741 gcgttgattc cattaaacac ttggatcaat gcttccaaag catttactcc tttttcaggt
    19801 gctgctgctg catgggaagc gacaccgtaa aatttgatgt cgactggatc attcgctaaa
    19861 gattctgtcg ttttcccata acgaaaagct ggatggacgc ataacgctac atcgacatcc
    19921 tcaaaaaaac cttctcgaac aaagctgcct ttagctgatc cgttctcgcc cccttcttcc
    19981 cctggggtgc catatacacg gatttctcca ccagtttcat caatgaattg cttcactatg
    20041 ctggcggcta atacggagta agtaccaaac aaattatgcc cgcatgcatg accaatacct
    20101 ggtaaagcat cgaattctgc caaaaaggca attgtgggac cgggtttatt ggaaatataa
    20161 cgtgcatcaa aggctgttcg gtgtccagct acttcttttt ctacccaaaa cctttctttt
    20221 tctaattgtt taatcaacgc tgctgaggaa taaaactcat agttacttac ttcaggatgt
    20281 tcgtggatat ctagagcaag tgcttgatat tctggtaaac gatattggat tagatccttc
    20341 aattggtttg ttgagattgt tgttgtcata aaaaattcct cctatgctag tcatttttct
    20401 tattttctcc cgaatgtttc ccatgcaggc acgcttgatc cttttgatgt tttctcgatt
    20461 actttgactg tgtcttctgt ttgataagcg tctactattt tttgatacgt tttattgttt
    20521 tcatcttctt ttcttgcaac gatgatattc acataaggac gtgcatggtc atccactggt
    20581 tctaagaaga tggcatcaga agcaggatta aatccagcat ctacagctac accgctattg
    20641 atcactgaaa tgtctacatc tgacaaagca cgtgctgttt gggaagcatc taattcagaa
    20701 atgtctagat tttttctatt ctcggtaata tcagaaacag tgggcgcttg tccttttgcc
    20761 ttatccactt tgatcaatcc agcagtttgc aataacagaa gagctctgcc gccatttgta
    20821 acatcattag ggattgctac ttttgctccg tctttgatat cagctacatc cttcaaagtg
    20881 ttagaataaa ttcccagcgg tgcattgaca gtatttccga tcgatactaa atcagttccg
    20941 tgttctttat tgtaattgtc taaaaagaat tgatgttgga aagaattcaa atcaatcgat
    21001 ccatctgcta aagccacatt tggttgattg taatctgtaa atttcacgta ttccaaatta
    21061 atgttctcct ttttcaaacg gtctttcacg ttgtcccata catctgtatc ttctccaata
    21121 atccctagtt ttacgatagt ttcttttttg tcagttgtat ctgcttttcc ttggctacac
    21181 cctgccaacc aaataattgc tacaaatgta atgactacaa aacgaataat ttttttcatt
    21241 ttttctcctc cactttcaat gtgtaatttt tttaatcagt tgattactga taaattgact
    21301 taaaaaaact aagattagaa tcaataatgt agccacaatc gttacatctg tttggaatcg
    21361 attataccct ctcgatatag caagattccc taatccgcct gcacctactg ctccagccat
    21421 ggtagtcagt ccgatcacgt tgatgatagt caacgccgag acccgaatga taccaggcaa
    21481 accttctaac aaatacacac gtaaaatgat accgattgga ctggttccca tcgcttctgc
    21541 cgcttcgatc accccaggat caacttctag cagtgcgttc tcgatttgac gagcaaaaaa
    21601 cggaacaact ccaaaaatca atggcactaa agctgctttc gcaccaatcg tcgttccaac
    21661 taagaaacga gtagtcaatg ctaaaagaga aagcaagata atgaaaggga tggaacgaat
    21721 gatattgatc agcttatcaa gtatttcaaa tagcagacgg ttagctagta atccattagg
    21781 gcgtgtcact actaacgtca cacctaagat gattccaatc atcccgccaa tgaatgctgt
    21841 ccaaaaagac atatatagtg tttgcttcgt cgcttcgaca aattcatccg gaatcaaata
    21901 aacattgggc aaatatgttt taaagaaatc agtcatttta tcttcctcct tcgatcactt
    21961 tgatacgact atctaaatcg tttttttgta aaatatttat ttttacacca gatttgatca
    22021 aataatccaa tgcttttttc cgctgtttgg tttctccagc taaagtaacg atcaaactcc
    22081 ctaacggcgt ttcttgtaat aactctacat tgccatataa aatattcgct gttacttgat
    22141 atttgctgta cagatgagcg attagaggtt cgttcgtctg atttccaata taacttaatt
    22201 ccaccagcca tttattggcg atctgatcgg caaaagcaga atgggcaatg atcgtttcta
    22261 atgcttggtc aagatgagta gctgttcgaa taaaatcttt ggtcaattct tcttcaggat
    22321 tgctgaagat ctggatgctg ctaccttttt caacgattct tccatcttcc ataaccgcta
    22381 ccttgttgca gatttctttg acaacttgca tctcatgagt gatcaggacg attgttaaac
    22441 ctaactggcg attcaatttt tttaacaatg caaggatctg aagggtcgtt ttcggatcaa
    22501 gtgcactggt tgcttcatca cataataata ccttaggttc attcgccaaa gctcgtgcga
    22561 ttgctactcg ttgtttttgt ccccccgaca gttgtttagg ataagcagat gctttttctt
    22621 ccaaaccgac aagttccagt agttcattca ctttttgacg acgttcctgt ttcgattttc
    22681 cagaatactt taaagaaaaa tcaacgttgt caaaaatagt tcggctttcc attagattga
    22741 aatgctgaaa gatcataccg atggcctttc tttcttttcg caattctttg ggagagagct
    22801 gactcagatt cgtctgattg acaatgactt ctcctgctgt tggtttctgc agcaaattca
    22861 tcacacgaac taacgtactt ttccctgcac ctgagtaacc gacgattcca taaacatcgc
    22921 ctttttcaat cgttaaatct acagaatcta ctgcctgtat ctctttttct ttttgtttga
    22981 aacatacaga aacatctcgc aattcaatca ttaaaattcc tccttatcta aaaaaagcgt
    23041 cctcaagaaa atgaaaatgt cattttcttg aggacgcttt tttgcgtgtt accacctcat
    23101 gttcaagctg tgttcacaca caactcctct tcaagtacga tcatactcta gcaagatatc
    23161 ggtcgcagcc gtcatcgcct tacatctatg ctcgggatga attgctcgat ggccattttc
    23221 caatagtcgt tccttgcttc ttctcagcta ccgaagctct ctgtcaaagg ttcttctatc
    23281 gtacttacca tttcaatgca aataatattc aaaaaaaaag tccttttact aacataaact
    23341 atgtcagtaa aaggacgacg tatcgcggta ccaccttttt tcggaagttt ccttccctca
    23401 agcccaacat ctcaaaaaga gagattggcg ttcgttttaa cggtcgaaca gttccgaatt
    23461 tgcctactta tcgacaaatt attctccaag gccattttca gcttttctta cttgactctt
    23521 ctcaccagcc agagcctctc tgcaaagttt ccaaacttac tttccttttc aacgaaatcg
    23581 atattttgtt gttgggatta atctacatga ggaaaactga aaagtcaagt acgaaattca
    23641 aattattttt tctatattta tttttctgtt tttttattta acatttcaat aacagttcga
    23701 agatcttttc cttccgcaag aagtttcaat ttttcctttt cttcgacggt ataatttcgg
    23761 acaaaactgg aatcaggtat caaaacaacg cctaatcctg ctcgtgaagc agctgttgct
    23821 cctactaaag aatcttccac tgcaaaggct gaactagctt ctaatcctgt tcgttccaaa
    23881 gctgtcagat aaggaatcgg tgaaggcttg ttctccgaca cttcgtctcc gaatgtaaac
    23941 gtgtccaagt aatcagctaa tttaaagtga tccaagatat ctgttgctct tttcgtaaca
    24001 gtggtcgtag caatgcctag tttcatatga cgttctttta agtctgccaa tatttctttt
    24061 gcatatggtt ttaagtggat ggctccgctt gtcagctgtt gataaaaata ttcttcacgc
    24121 ttcgctcgga cttcctgtac tttttcaggg ctacctgctg ctttcactaa aatatcaaaa
    24181 ctttgtttcc agttttttcc gccccaagcg tcaacgattt cttttgggat atggatatca
    24241 aattgctgaa atccccaaag ccagccatca cgatacgttt tttctgtgtc aactaacaaa
    24301 ccatctaaat caaatattgc acctttcatc tatacattaa tcctttccaa aacaatacac
    24361 atagtttagc acgactggtc caaaaagaac agaagcaaag aaccagcctt ctcgtttata
    24421 agagttcttg gaatcgacgg atatagattt tcttcacagc tgtcacaagc gccagataag
    24481 cgatgactgt agcaaataac cataaccagt aattgccagg aagtggcatc aagcctaatt
    24541 cagcaccaaa acttgtaaaa ggaaggacag aaccgacaat aattcctaat gtagtgataa
    24601 caaacatggc aaatgttgcg ttgctttgga taaatggtac tttcggtgta cgcagtgcat
    24661 ggagcactaa ggtttgtgtc catagtgatt cgacaaacca tcctgcatgg aataatgcga
    24721 tgaaaatcac tcgctgtgaa ctatccaatg tatggaaact tccgcccact acagcaggac
    24781 aaataacaaa atacatcaac aagtaagtag taatatcaaa tatcgaactt gttggaccaa
    24841 agtaaaccat gaattcccca atactagatg cattccattt ttttggctcg tccaaatatt
    24901 ctttatccat ccgatcccat ggcaaggaaa tgcaggaaac atcatagatc agattcaaaa
    24961 acaaaagttg taaaggcagc attggtaaaa atggcaggaa cgtacttgca atcagcacgg
    25021 aaaacatatt gccaaaatta gaacttgctg tagccttgat atatttcatg atattcccaa
    25081 aagtttctcg tccagaaaga aggcctcttt ccaaaatcgt caaatctttt tctaaaagga
    25141 tcacgtctgc agattctttc gcaatatcca cagctgtatc tacagaaata ccgatatcag
    25201 ccactttcat tgcaggagcg tcattgatac catctcctaa aaatccgaca gtatgaccat
    25261 ttttacgtaa agctgtcgtc aaacgtgctt tttgctgagg attcaatttt acgaatactt
    25321 catggtcttc agctacttgt gctaattcat gatcactcat ttccgagatt ttctcaccgg
    25381 taaccaattc attgatctct agacctactt gacggcacac tgaacgtgta actaattcgt
    25441 tgtccccagt cagaactttt acagctacac catgttcttt cagagctttc aacgcttgtt
    25501 tcgttgtttc ttttggtgga tcaaggaaag caagataacc aatcaaaacc atgtctgact
    25561 catctttgac tgaaaattct ccaacgacgc ttggattggt cttttgtgcg acaccaatca
    25621 ctcgtaagcc atcttcatta agtgactgta cttttgcaag tacggcttct tttcgttctt
    25681 ttgttaatgg tgtaacagta ccgccaagat caataaattt agaaatgttc agcatttctt
    25741 caatcgcgcc ttttgtgatc atttgtgttt taccagctgg atcagcaacc acaacactca
    25801 tacgacgacg ttcaaaatca aaaggaattt catccacttt ttgataattg atcttatctg
    25861 tatctaaagt ttctgttgct tcatcaatga tagccacatc taacaaattt ttcagccctg
    25921 tttgatagta gctatttaga tacgcatggc gcaaaacacg atcatcttca tttccttcac
    25981 aatccatatg atattcaaga atgattttat cttgtgtcaa tgtccccgtt ttatccgtac
    26041 ataacacatc gattgcacca aagttctgga tggcattcaa atttttgatc actgttcctt
    26101 ttttggccat cacacttgct cctttgacca aattcgtcgt aacgatcatt ggaagcattt
    26161 caggtgttag accaactgct acagatagtc caaaaagaaa agcttctagc caatcccctt
    26221 ttgtaagccc attgatgacg ataactgctg gagccatcaa tgacataaat ttgatcagta
    26281 gcatggatgt tttatggatg cctaattcaa agcttgattg aattggtttt tcagatagcg
    26341 tttctgctac atgaccaaat agtgtttcat ttccaacaga aacaatgatt ccttccgcac
    26401 ttccactgat gacattactt cccataaaga caagattttc gtaacttgtt tcgctttgct
    26461 gatcgtcttc acatactttc gcatgctttt ctactggata gctttcccct gtcaatgctg
    26521 cttgggaaat gaataaatcc tttgcggaca ataaacgcat atccgctggt atcatatccc
    26581 ctgctgaaag ccgaacaaga tctccacaga cgatttcttc cataggaatc tctgtgtact
    26641 ttccattacg ttttacagct gccgttactt tcacaagtga tttcagtttt tcagccgctt
    26701 gattggaacg tacggactga atcaacgtca ttgtcccgct gacgataacc atagtgaaga
    26761 taatggctac accgaaaaga tctttatctc caggagctgc taatacatat tccgtgatga
    26821 atgaaataat tcctaatgcg atcaacacta atgtaaatgg cgtgatgtat gctttcaata
    26881 tttctacaat gaatggtgtt tttttgccat atgaaatcgc attttcaccg taattttctc
    26941 ttgaacgttc gatttgttct tctgtcaatc catttgctga tgcatggaat agttcaaata
    27001 attcttgttt cgtttttcca gcaaaagctg catagatttt ttgtgtatct ttcttttttt
    27061 ccatctcaat ctctcctttg tgtccaaaag acagctgttc tcgctgcttt tggtctttag
    27121 ctattcatcg gatcgataga gaaaaaactg aaaggggctg agaacagata gataatagcc
    27181 ctggcctttc ctctagatcc ttgtttttcg aatgactgtc atccatttgt tctcatctcc
    27241 tttactaagt aattttaatt catgacttcc aatcattttt cgaatagtta tttttccaat
    27301 tagaccacct cctttttatt tttcaccgat ggggatttgc ggcaacaaaa aaacctctcg
    27361 ccaaatactc agcgaaaggt ttgaaaaagc atattaaaaa aagcttctca gatctcatcg
    27421 ttgagttttg acactgtaca acgtaaaaag cagatacctg cttttagcta ccttatgaaa
    27481 agccttatct cgacaattcc tgttctaccc attggcgtct ctcgacgttt ttgggcagta
    27541 gcctatgttt gtataggagc ctcacctaac aaatcttatt cggttttcct tcgttaatat
    27601 aacaactaaa aaagaggatg tcaaatcaat tttccatgtt tttcatactt tttttaaaat
    27661 acacacgtta tattcctgtc tcacgcggtt ttatttgtct cattttccac ctcccgctat
    27721 aatagaaata tagtaggaaa cgaggtgctg ttatggaaag aacacttgtt attatcaagc
    27781 cagacggcgt gagacgccat ttagtcggaa gcatcatcca acgttttgaa gcaaagggac
    27841 tggccattgc tgaaatgaag tttgaaacta tgactcctga attagcaaaa gaacattatc
    27901 agcacttgac tgagcgcagt ttttttgatg aactgatcga ttacatgact tccggtcctg
    27961 tcgtttatct tgtgctggtc ggcgaggaag tgatcgatat cgtccgtaag atggttggag
    28021 caacaaaagc tgccgatgct gttccaggta cgatccgagg agactacgcg ctgccgggga
    28081 ctgaaaacat cattcacgct tctgattccc gtgatgctgc ggtaaaagag atcgcccgct
    28141 ttttcccgcc attcgatata aacaaaccgt ctgaaaaagc ggcgaagtga tacaaattct
    28201 aaactgctgg tctagttgct gggttcagca atggccagca gttttttctc taaggacctc
    28261 cgttgattct ttcctaacat atattcgagc actgtgaaga attcttggta gtctttcgtc
    28321 ttttcttccc ctaaagcagc agaaatcaat ctttgataat aatctgccat caatgtttca
    28381 tagatcactg tacttctttc tttatgtgat aagctccgtt tcatatcttc attcacacta
    28441 cgtgcctttt tcaaccgacc attttctata taaaacgaag tcaggttcat taaaaaagta
    28501 aagagatact gctcatgatt gttcaactgt gctaatcgtc gcattctttt tttgattttc
    28561 aaataattgt tctcaatgaa ccgatcactg aatatccaag gaaaatcagc tagaatagat
    28621 agttcaaatt gaccatagtc tggtatctgc atcaagtatt cttcaatgat tcggcattca
    28681 taacgtaaaa attcaaattc tgtctcagat acttctgttt tttcgaccat gtgaagattc
    28741 aaaaccaaat aatacagatc ttctgttttc ttaaaatgcc tacggttatt gctgatctct
    28801 ttataaggat caaagctgtc ttcttgtacc cgatctagca ttcgtctttt tttctttaac
    28861 tttattggtt catccagctg gtttcgcatc cattcatatt ctgcaagtgt catgttcatt
    28921 cgctctaaaa atgatctcaa tgttacaaat gacatttttt tcactgaatt tgtctcaatc
    28981 ccgactagag aagtcttaaa acagattcct tccgctaatt gggactgtgt caacttcttg
    29041 ttgactcgaa gtttcttgat caattcacca tcatacataa tagttctcct ttttaattta
    29101 aatttttcca cattcaataa atagatacgt taaaaaggaa aaatacacga aaaaaataaa
    29161 aaaatgtttt tttcataaaa aataccaacc ttcaagcatt agaatgttgt ctaactactg
    29221 aaggttggtt tgattgttta ttgattattt ttttgctgtt caacgtctgt tcttgttact
    29281 ggatataagt tggttgttgt tttattacca ttttttgaaa agacacttgt cgaatgttcg
    29341 cctttttgct tttcgattgc ttcattacgt tccgcttcat ttttatagtc atattttttc
    29401 ggatcaatcg tctcaaatcc gtttggcaca taaaaccgca agagattttc ctgattcagc
    29461 ttatctgaaa gacttaacgc agtcttgact ttttcctgat ctttggagat ttcctcatcg
    29521 attcctgctg ctttcgtatc gatgatttct cctgtctgat tattgtaagc ttttcctcct
    29581 aggacggtgt atttgggtgt cacaaagttc ccatttcgaa aagcaactac ttgatcatgc
    29641 tgagaagata ataagtctgt accaaaatga agataatctt tatcatcgat tcccaataga
    29701 tgtagtaatg tcggcaaaac atcgatttct ccaccgtatt catgattgac tgtcccttta
    29761 gtataaccag gcatatggat catcaatggc actcgttgca tctgtgcgtt gtcaaattca
    29821 tcccatgtat ctggatcttt accaagagcg cttgctaaat ctttattatc tgtattcgag
    29881 atcccaaaat gatccccgta aatcacaaag attgaatttt ggtaaatacc agattttttc
    29941 aaatacgtaa aaaattgttc caatgattga tccagataat gtgccgttcg aacataatta
    30001 tctactgtgc tattgccagt attcaaagat ggaaagtcga aattcttgtc atccgtataa
    30061 taaggcgtgt ggttggttac ggaaagaaat ttcgtgtaaa acggctgttg tagatgctca
    30121 agatattggg cagattctct gaataaatct ttatctaata tcccataacc taacgtttcg
    30181 tcggattcat caaaataaga tcgatcaaag aaattgtcat aacctaagtt tttatataca
    30241 tgatctctat tccaaaaact agctacattc ccatggaaaa cagcgcttgt atagccttgc
    30301 ttttgtccaa ggatcgctgg tgcggcttga aagacattgt ctgacccaag ttctgtgaat
    30361 aatgagcctt gaggtaaacc aaatgtgcca gtttccaaca tattttcagc atcacttgtc
    30421 tttccctgtc ctacttcatg aaagaaattg tcaaagctga tcgttgcttg attctggaaa
    30481 atactattta aaaacggtgt gacttcttgt ccgtctacct tcatattaat caaaaactgc
    30541 tggaaacttt ctaaatggag aacgatcaca tttttccctt tagcgatccc aaatgtttcg
    30601 ggatttggct ctgcataatg tttctttgta taatctaaaa cttcatcaat cccattactg
    30661 cttgcatgcg cgcgaacact gctagtcatt cctgttttga ttccgtcata aatagtatac
    30721 gcatcgattc ctagatattt gacaatatat gaacggtcaa atgttcgctg cagcaattgt
    30781 ggacggttgg cttcgctcag cgctagattt cctgaaaaaa taagccctga aagacaggta
    30841 acagcgatag ccatcggttt gccgacaggg ttgcttttga taggtatttt cttgaccttc
    30901 aaccaaacca gtagcccaat gaacacagcg atatccagcc agtagatgac atcatggggc
    30961 ttcatcaaag aaaaggagct tcctgacaat ccttgcgaca ctttcgaaaa gcctaataca
    31021 gatttgactg taatgaaatc tgtgaattcc cgataaaaaa taatattcaa gtacaacaaa
    31081 agcgtattta aaatatcaat gatcaaaaga atagctaacg ctggctttgg ctttttaata
    31141 tacaaagaaa ggccaaaaaa caataaggta gtcgcaattg ggttgatcca caagataaag
    31201 tactggatcg taccttccac acctaatgaa aaatctgcat agtaggcaat gatcgtcttc
    31261 agccagaaaa agaaaacagc tgtcaaaacg attgctaaac gattttggca caatttttta
    31321 agttgtgtca cgtatatcac ttctctctat tcacattttt tcttattttg ctcctgaaaa
    31381 atgaaaattt cctaaaaaag ttgttatctt aagatttttt tattctttct aaagattatt
    31441 ccgtccactt ttcacagcat aaaaccgctg cgatgtaagc gattaaacta atgaagagat
    31501 aaaagtcttg tggtgaccgc ttcactccac ggtattcaaa agccagtttc tcggtattcg
    31561 tacaaaaaaa gaggctgtga caaaattctt gtcgcagctc cttatttctt gatgcaggac
    31621 agctttttca caacttcttc cttaaacggt gttctcctgc caactcctcg gccacaagtc
    31681 acaatattta taaaacatga gaagctgttt tttgaaattg ttccttgtgg ctcagagtta
    31741 aacggcaggc tcaagacctc ttgatacacg gtgttcaatc agctgaccct cggtattaga
    31801 ttatgtcagg acgctaattt tacaacctct tggtaaacgg tgttcaaaag cttgcatctg
    31861 cggtaataag cccaaccaaa cgaaaaatat aaggagctat tttttgaaat tgctccttgt
    31921 ggctcagagt taaacggcag gctcaagacc tcttgataca cggtgttcaa tcagctgacc
    31981 ttcggtatta gctcaaaaaa cggcaaaata tgaggaacta ttttgccgtt tgtttctcta
    32041 atactcaggt caaaacgctg atttcacaac ctctgttttt cattgtccat aggattgata
    32101 ttgtccttgt gatgactcat tcgtaataat tgaggcttga ttttcttcac ttcccgttat
    32161 cgtttgtcca ctgccagaat ctgaaggact tgttcctgtc gactcgtttt tttgagaaga
    32221 gacagttgtt ttcttgactt gttcatttgt ctttttccat tccgctaaac gatcggcgac
    32281 cacttttttg tctacttttt cagaataagc ggtgatcact tttccatttt tcaaacgggc
    32341 tactttgtcg ccatcatgag tcaaaagcaa aacggtcaaa tgcttgtctt taaattgata
    32401 ttgtctgaga gaaatcgcac taagtacgtc atcgatatta ataaattgat tttcttcttt
    32461 tccttttggc aaataaatgt ccaattcgcc tttttcgtaa ttggcagtta ttttatactc
    32521 cgcccaccaa taagttgaga attctttttt gaaattatct actgattctt tgcttgctac
    32581 gattttttct gtttctttct gagcaagatc tgctgtcatt tttcctgaaa atgctgcgga
    32641 taaaataagc agtaagaaaa aaccgaataa ccaaaagaga agaaacggtc tttttttcct
    32701 catgctttcc tccactccta tttaattttg actattctat tatatacaaa aaatgaaata
    32761 gaaagacaaa aaagttgaaa aagatcgtaa ataattgaac ttctttttca caaaatggta
    32821 agaatggaga atttcacgca tttgctctca actattcttt cttaagcctc aaatccgttt
    32881 ttatctgata cgtctttgaa ccaatgaccc gattttttga ttgttctttt tccatcttgt
    32941 gctaagtcta ctgaaataaa gccatatcga ttcttatatg cattggtcca cgaccagtta
    33001 tccatacatg tccacatatg atagcctaaa caattggcac cttcttgaat ggcttgatgg
    33061 acatatttca gatgagattg gacaaattcg atccgataat catcgtggat catgccatct
    33121 tctttgataa atcgtgcttc gttttctaca cccataccgt tttcagaaat aaagcagtcg
    33181 atatttccat aatgatctcg tacattgatc aaaatatcat agattccttg ttcatagatc
    33241 tcccatccgc gataaggatt cattttacga ccaggcattt cataattgtc gaaataatcc
    33301 tcaggcattg gaccgttttt agtatcgatt ggtgtttctt ttgcttttac ccggcgtggc
    33361 tggtaataat tgatacctaa aagatctact gtattttctt tgataaccgt caaatcctct
    33421 gcatggatca caggcatgag atcaagttct tttactatct caatcaattc acttggaaac
    33481 tcgcctttta cagctggatc aaggaatgaa cgattgaaga acgcatctgc tatttgcgct
    33541 gctttaacat cttcagggtt tgattcgtct cttggatagc ttggtgtcag attcaagatg
    33601 ataccaataa caccaccttg ctccatttca tggtaagatt tgatggctaa cgctgaagcc
    33661 aacgcttcat gatagccaac ttggatcgct tttttcatat ctgactcttc tggataatgc
    33721 catcgatata aataacctgc ttcgactgga acgattggtt cattatgagt aaaccatttt
    33781 tttactttcg aaccaaacaa ttcaaaacaa cttttggcat atttttcata ggcttcaacc
    33841 gtttctcggt tcaaccatcc gcctttttct tgcagataca tcggcatatc gaaatgatac
    33901 agattcacaa agggttcgat accgttttca atcaactcat caaaataacg atgatagaaa
    33961 cgaacggctt cttcgttgat tgttccagat tcatcaggta tcaaacggct ccattggata
    34021 gatgttcgaa atgaattatg cccagtctgt ttcatcaact gtacatcctc ttgatacttc
    34081 gtataaactt gcgaggtttt ttcaggtcct acttgatcaa agaacttttg cggttctttt
    34141 ttgtaccaat aatcaaaaat gttctctcct tttccatcgc ctgaaatcct tccttctgtt
    34201 tgtgggccac ttgctgcaga accccaccaa aaatttgctg gaaattgata ttttttcatc
    34261 aaagcacctc tttttctttt ttagttcgtt ttagttgatt ccatgtatga tattttccat
    34321 cacaatcact ttttcatttt cttcctcttc ttttttgatt cgttttaaca gctgttctgc
    34381 tgctcgttcc ccgattttat ctgttggtgg cgtgatcgtg attttcggaa ggatactcag
    34441 atcgttttta tctaaataat catagtgact gaacgcaata tccttcccga ttctgacacc
    34501 ttcttgattg aaacattcga tggcaccaat cgacatattg taattgctga caaaaatagc
    34561 agttgcccct ttttctaata atgcttttgc cccgtaatag ccgctttttc tagaatagtc
    34621 tcctgtaaag acctctaatt ggatcgatgg atcattttgg accgcttctt tggctcctgc
    34681 taaacgttct cttgctacgt agtctgtttg agtggcagca ataaatccga tttttttatg
    34741 tccttgttca ccatgatgag cgatcacttt tgctacactt tctcgatcat tcactaatat
    34801 tgtatcaata cctgctggtc cttctggaca attcaatgcg ataactggaa tactcatttt
    34861 tgtgagcatt tcaaatccag accactcttc ttcggataag aagacaatca gcccatcgat
    34921 cacatgcttc atcaggtatt caattttttg ttcaattttt tctggctgat cggcaaaccc
    34981 gcttaataat aagctatacc cattttcttc agctactctt tcgatccctg agacaacttc
    35041 cgacccaaaa cggctggaaa tcccgtttat caaaagtcca atagagaagc ttcgattatt
    35101 cttcaaccct ttagcaatga tgttttcttt atagtccaac gctgaaatcg cttctgctat
    35161 ttttttcatg ttcttttcct tcagttgctg accattcaag taacgggaaa ctgttcccac
    35221 tgaaacacca gccatcttag ccacatcacg gatcgatgcc atacattctc actcctgcca
    35281 gttacatttg atttctcttt cttgttgttc gattatgatg accgcattct ttgcatagtg
    35341 ttgcttgtca tcaatatcca tataactaaa gtcttcagta gagcaacttc ctctcactat
    35401 taattatact taaacaagta tttgaaccgg ttcattttta atatagcatt tttttaaaag
    35461 attgcaacac ttttttaaaa cgctttctga aagtggcgga ttttttgcct ttttaaaatt
    35521 ttattctaaa atttactcta tttgtgggtg ttttgtgcgt ttttctcttc catccgtttg
    35581 aagttttaca ttccaatggt ataatttctt tatcctatca aatgggggaa gtattttgaa
    35641 gaaattaatc gtactattta ttttgtctgc gggacttttg acattgactg gctgtgattt
    35701 gtttggcggc tctgaccaat cctcggacga aacaaccgat tcttctgtct taaaagcgga
    35761 aaaagcagat accaccaaca gtgtatatac gctaaaagta cgtaaagata tggatttatc
    35821 cgcgggacag ttgacattta cagataaaaa aatggaatgg acacgcacgt atcagacaca
    35881 aggaactgat tcttctgaat catctgaaga aaaaacagtg ctgacagata tcaagatcac
    35941 taccaaagat gacgaatata ttatttcagg aaaagaaaat aaaaaagaaa caacagtcat
    36001 atttacaaaa atcggtaatt accgtttgaa ggatgaggat ggcaacatct acagtctata
    36061 gtgccgtctg ttggatcttc ttttgtttct ccccttcttt tctgctatag taaagaagca
    36121 agatgattgt aaaggagacg aaatggatga tcgatagtcc tgattattgt acatgtaata
    36181 acaacaaaaa tatccataca gaatttaatg accaaggaag cttgagcata tgcgacaact
    36241 gtcacaaact agtcaaagat tcatttaaaa catttgaatc ttatcctgtt caaaattatg
    36301 taaaaagtag aagaagataa acaaatccaa gcaaaactca ctatggctga tttttatgaa
    36361 cttcaaactc agcttcttcc tttgatcaag cttcatcagt aaaacaaaca gtggatcatt
    36421 tcagtttaca aatcaaagag taaagattta tttatttcaa atatttgaat ataaaataac
    36481 caaacgatag aaaggcttat tctacatcac gtagtaattt ttctttcccg tcgtttggtt
    36541 ttttattttt agcctaattc ttgacctttt gcatgctggt tatccgtaaa atattttcca
    36601 gcaggctttc cttccaattc agctaatttt tcttggatct gacggatcac cttttttgca
    36661 tcttctggat tttctacaaa atcgaattta tccccatcca cttgtatttt aggacataca
    36721 tcaaaatctt cataccattg attatagcgg gtgttcaacg tttcataata ggtatataac
    36781 gtctcatcgc tttctagctg ttcatagtca cgcccacgtt ttttgatacg ttctagcatc
    36841 gtgtccaaag aaactttgat atggaccatc aaatcaggcc gtttttttgg agcaacatca
    36901 tctaactcgt ttagcatcgt atctaataag ctatcgtact gctgtacttc gatatccgtt
    36961 acccgaccta aatcagcgtt tagatggaac aacaggctat cttcatagat ggatcggtct
    37021 agcacattgt catcgttcac taaagcattt ttcattgcta aaaatcgctt gttcaaaaag
    37081 aagatttgca ataaaaaagc gtactgctca ggatttgaat aaaagagcgg caatacttcg
    37141 ttatcttcga cattttcata aaatggttta gaatcaatct cttttgcaag catatccgtc
    37201 aaactgctct tacctgcacc aatcgttcct gaaatcaaaa tcactgacat acaacccttc
    37261 ctcttctata agtactcgcc tacgtaagtt tcaacaggca atgtttcaaa atcatattca
    37321 cgtacagctt ggtctgattt taaatacgta tggagacttt gtgtaatcat caagttcgta
    37381 ggtaccgtct cttcttgtac aatgatcatc ggttttttca acatagttgc cacacctaat
    37441 tcgtaagccg ttcctggatc gattgtttga tgctcaaagt caaggatagc aacaacaaat
    37501 tctgcatttg tcagttcttc catatctttt tcgtatactt cctgcgccca tccagcgctg
    37561 aatagttcat aattgctttc ttggtgatga cgtggagagt agaaacttgt gactgttttg
    37621 ttttcctcca acgctttttc gatacgactt actcgatcga tttgctcctc tgaaaaaaat
    37681 ggtcctgcta aatatatttg actcattatt ttgcctcctt caataataac tgatttttaa
    37741 tagatactgc tcgtaattca ttgaccagtt ccaatggatt gttggaaaag attacttgat
    37801 ccaaccattt tttatccata aaatcctctt ttgcaaaagt ttctaataat tgttgcaagc
    37861 cgctgtaata accgccaata ttcaatattc cgattggctt gtcgggcgtc agaccgattg
    37921 ctatccaaga aagaagttgt gagaattcct ccaatgtacc tagacctcca gggaaaacga
    37981 ggacagcatc tcccttttca aataataata cctttctttc atcgatcgtt tctgtaggaa
    38041 taaatgtagt aacatctttt ccaggcaatt cagtttcaaa aagcccattc ggatagatac
    38101 ctgtgacttt tccgttattt tccagtactg ttttactaat cactcccata atcccggagt
    38161 atgaaccacc gtaaaccagt tcgatttctt cttgtgccat gtattttcct aacgtttgcg
    38221 caataaattt gtaactttct ttattaccaa aacgggaacc gcaaaaaaca gtaagtttca
    38281 taaaaaaaca ctccctaact caaaagtcta tcttcattct acacgtgttt ttttaaaata
    38341 aaagactaga ttttgtagtt ttttagtggt atataggata gacgccacta tatatagtat
    38401 ttagaaaaaa atcatttttc agccatttta tagataaaac aaaaataaat aacgtgtttt
    38461 catcgatatg aaatgactta cttatagaag atactgggat ttcgtcctat atgccaataa
    38521 cgtgtattat tttaaacaac aaatacttgt ttcttttatt aacgaaaaaa tcattttgtt
    38581 ctcgtgttat gtttcactga taaatgatgt ttctctaaat aaaaaagccg acgaactaaa
    38641 cgatcaaaat ttgatcattt ttgttcatca gcttctccta aaaatctttt tgttaacgat
    38701 aaatagctgg atttgtaaaa atatcacttg aatcgtcact gctggaagaa aattcagtga
    38761 taaccgcacc tgttgcgcct gcctggaact agtgtttgat attgggctcg aacgtgtact
    38821 gatctctagc atgaagaata atttcttttt ttactgtata ataactttct ttcctttttg
    38881 gaatagtaca ggaaagtttt tttgtttcct ttccttcgac ataaggtata ccagaccctt
    38941 tcgacaacga aaggttctca tttttccttc atcgtcattt caaggagggt gccgatgctc
    39001 tggacagttt cgatcgctcc taaaccaaaa tctgtatatt cgattctttc taactctgat
    39061 tctttcatac cattcctgtc tcaaggtaca tctattgtac ttttttcttg taatccattc
    39121 tgcctctcct aactaattgt caaatcatcc caagatatgt cttcttcctc tttttcttct
    39181 tggatgattt ctgcattcgg atctactggt tttttcaata ataaaatggt tattcctgtg
    39241 acaaaagaac cagctaacaa tgcaccaatg aatgcccatg gtctggtcat cgtagcgact
    39301 gcaaacattc ccgatgccgg aatacggcta tctgcgcccc aaaacatgct taatccgcca
    39361 cctactgtcc caccgactcc cgttgctatg acattgcgtg ccaagtcgtt caatacgata
    39421 gggaaacatc cctctgtgat ctgacagatc cccataggaa aagcggtctt caatgtatcg
    39481 atttcttgac gattgagtat atttttacga atgattttcc ctaagaaata agcgatcgtc
    39541 aagccgaatg gggttaccat cgaagctaaa atcaatacag tgattggttc atagattcct
    39601 tctgaaaatc gtgccacacc cccttatcca gaataaatgg taatccagaa acagccgtgt
    39661 atctgcggta ataagcctaa ccaaacgaaa aatatgagaa gctatttttg tttggctgtt
    39721 cttattactt gatgcagggc agctttttca caacctcttt ttttatttat gtttcataat
    39781 caatatggta aggatgcagc tccgtatggt agaccgcctc cgaatattca atcatttccc
    39841 caatttgcgt agacgatttc cgtatccgtt tgatgccgat ctgtgcgtct gttttcagaa
    39901 ttccttgttc ttcttctgtt aaaaatactg ccgagaaatc atcagaaaat gagtcaatag
    39961 tatatccttt tttcgtcagc aaacgataca aagattcttt gccaaattct tctctgctga
    40021 attgcatcat ttcttgtgga agataatgaa caaaataaat ataaggctga ttgttcaaat
    40081 aatataagcg cttaaaacaa acagcttgct caccgaaaaa ggaaaacaaa ggatcttcct
    40141 tctttatatg gaccaattgc aaatccagcg tttctttccg gacttcatat cgggagttct
    40201 ccaagatttg cgtaaagctt gccgctttgg ataatcgatt gtaaggacga ttgcttagaa
    40261 ccgtcgtccc acgaccactt ttctttgcga catattcatc tgatgccagc agttcgattg
    40321 cctttcggac agtgattttg ctgacttgaa acagttcttc taattcactt tctgtcggca
    40381 acatcgaacc gactggatat tttcctgaga aaatatcttc tttaatttta tctgcgactt
    40441 ccaagtatag tacacgtgtc cttttcataa cgtcaccctt tgctttacta ttactaaaag
    40501 aatagcacat aaaaaataaa aaaagaacac aaatcacatg tgagaagtgt gttctttttc
    40561 attcagctgc tttaattaca agtttacgcc atttgaggca attacttctt tataccaatc
    40621 aaaagatttt ttctttgatc gattcagtgt cccatttcct tcatcgtcta gatccacata
    40681 gataaagccg taacgtttac tcatttctcc tgttgatgca ctgacaagat cgatacatcc
    40741 ccaaggcgta tagcccatca aatctacacc gtctgctact gcattagcca tcgcttcgat
    40801 atgacgacgt aaataatcga tccgataatc atcaataatc gtgccatcct cttccatatg
    40861 atcaatggct ccaagaccgt tttcaacaat gaataatggt ttctggtaac gattgtataa
    40921 ttcattcaat gcgattctca gcccttctgg atcgatctgc cagccccatt cacttgcctc
    40981 aagaaacgga ttttttactc ctcccagcaa gttcccttct gaagttgctg cttgtggatc
    41041 agtctcattg attgccgtag acatataata actaaatccg atgtagtcca ctggatattc
    41101 ttttagcaac tggaagtctt tttccgtttg ctctaatttt tccggatcga ctccatattt
    41161 tttatgggta cgcgctgtgt atgcagggta ttctccacgt acttggacat ccgtgcagaa
    41221 aaaattgaat tcttggtttt ggatcattgt tgctgcctga ttgactggat tggcatcgat
    41281 gctgtaagtt gttgcataga taatcatgca gccgatttgg atatccgaac gcaattcgtg
    41341 gccgattttt acggctttgc tacttgcaac gaattgatta tgccatgctt ggaaaatatt
    41401 ttggtactct cctgctccat tgctttttac taatccttga cttaaagctg gaaagtgaaa
    41461 ggccgaattg atttcattga aagtcatcca atatttgact ttactgctgt atctctctaa
    41521 tactgtctga gcaaatcgtt catagaagtc gatcagtttt cgatttttcc agccaccgta
    41581 ttcttttgcc aaatgaagag gcatttcgta atgagaaatc gtgatcacag gttcaatatc
    41641 atattttaag cactcatcaa tcagctgatc ataaaattct aatcccgctt cgttaggagt
    41701 accttcgtct ccatttggaa aaattcgtgt ccaggcaatc gaaaaacgat aacatttgaa
    41761 tcccatttcc gcaaacaagg caagatcttc tttgaaacga tcgtaatgat cgattcctcg
    41821 atgattcggg taaatatatt tttcttgatc aatcgtccag tcaaattctt cactgccgat
    41881 aatcgcaaag cgctgcttac ctccaggcat agcatctgca acggatagtc cttttccgtc
    41941 tcgatcataa gccccttcta attgatttgg cagcagttgc tccaccccat aaaaattttt
    42001 ctgaaaaacg tgattgcatc ctgatttcct ccttatttta caattgccag aacatcttcg
    42061 tttgcaataa tctctttttg gtctaattca agtatatcgg caaatgcatc agtattcgta
    42121 atgacaactg gagtgattac gctatagcca gctgctctaa ttgcttcgat gtcaaaacga
    42181 atcaataaat ctcctgcttt gacgagatcc ccttgtttga ctaagatctc aaagccattc
    42241 ccatttaatt ctactgtatc catcccgata tgaatcaaaa tctccgctcc gtcagtcgtt
    42301 gtgatcccta cagcatgtcc agttggaaaa atagtcgtga tctctccgtc tgcaggtgca
    42361 actagttctc ctacttctgg atcaatagcg ataccttttc ccatcacgcc agaagaaaaa
    42421 acttggtctg gtacttcact caatggcagt actttacccg ttaaaggact ttttaaggta
    42481 tgccgtgctg aagtaatcgg ttctcctgca ttcgcatgtt tgttttctaa ttgattgtct
    42541 tgtgtttgtt cgtcaaatcc taaaatcaat gtacctacga atgcaagaac aaaagcaatt
    42601 cctgtagcta ttactgctac tgtcacattt gactctacac catccaccgt attaatgaaa
    42661 gtcggaatcg tcaataagct gaccaaactg ctggagaaag cttttactcc ggaaaatccg
    42721 ataatagctc caccaatacc tccagaaata caagcggcaa tgaatggttt cttcaaaggt
    42781 aatgtcacac catacacagt tggttcagtg attccaaata acgaagtcac tgtagaagaa
    42841 atccctaatg ctcgaagttt cgtatctttt gttcgcaatg ctacagctaa agcagcccca
    42901 ccttgtgcca agatggctgg aagaagcatt ggcatcaata catcaaaccc atattgttcg
    42961 atattcaaaa acatgatcgg tacgaatccc caatgcatgc cgaacatcac gaatacttgc
    43021 cataaacttc ccatgattag acctgctacg attggactaa atccataaat cgagttgaag
    43081 agaccgccta aagcattacc ggcaaccgtc ccgattggtc ctaaagcaag aaatgttaat
    43141 ggaaccatca ctactaaaac gacaagcgga actaaaatca tttgcagaaa ttgcggaatc
    43201 acttttttta cgaatggctc taatttactt tgaacccata cggctagaat gattggtatg
    43261 acagaagaag tataaccaga tggtgaaaga atcactggga tcccaaagaa actgatagct
    43321 tctcccgcac cagcaactgc tgtgatagct ggatacacaa gtgccatagc aattgctact
    43381 gctaaaaact ggttggcttt gaacttttta gctgctgtaa aagcaagcat gacaggtaag
    43441 aaggtaaaga caccatctgc tgcagcaaac aatatttgat aagtaccgct atcagctgtc
    43501 agccagccca tcaccgttgc caatgataag aatcctttca ataccccggc ggctgccatc
    43561 gcccctagaa aaggtgtaaa tatactagag ataatatcga tcagacgatt caaaatattt
    43621 ttggactctt cttttccatc ctccgaaact gcaagacctt cctcatccac gatcgattga
    43681 taaacatctg ctacattact gccaatcact acttgatact gaccgccact ttgtacgact
    43741 tggatcacgt ccgggtcttc ttttaacttt tgcgtatctg ctaaagattc atcttttaat
    43801 ctgaatcgca accgcgtagc acaatgtact aaacttttga cattcttttg tcctcctacc
    43861 gcttcccata cacgacgacc aactgcttga ttatccattg ttttttcctt ctttcttttt
    43921 tatttaaaaa ataaaaaccc agattgaaaa aaacagtcac aaacattcgt gaacctattt
    43981 tttcaatcca ggttttgcct gccatacagt aacaatcctt atgattggat gctttttttc
    44041 tcacttacta ctctttgaat atgaatcgtt aaataaatct gctctgcgat ccccatttcc
    44101 tgtttttgcg tttgtcgaag atactcattg atccgttcga cacaatgaaa cgcttttgga
    44161 tactttcctt gaactaatgc gtagagaaat tcatcttgct cgtcagatcc ttcccctttg
    44221 agataacgct gggcaaaaaa ctgcagatga gtaatgatac gctgataatt caaagattct
    44281 tcatcgaaca cgatgccgaa gaatttactg atgatactca gtatatctct taccattgtg
    44341 gtagcggtca tcgtcacttg catgtcttcg tttccttgtt ggctattcac aaaatgaaag
    44401 gcaataaaac cggcttcatc ctcagaaagt tccacacccg tcttttgttt cacgaacaaa
    44461 agagcctctt tcgctgcttg atattctttc ggataaaatt tttttgtttc aaataataat
    44521 ggattaggca gttggatatt ttcttttgct cgtgtgatcg cataatgaag atgatcggtc
    44581 aatgtcagat aaatattagg cgaaaattca acttttagtt tatcttcagc cagttcgact
    44641 acttgtttga cccattcgat ctcctctgcc ggaatatcag ccatcagctg ctggaattgg
    44701 tttttagcaa aagaatctct taaacaaatt ctttttggac cgcttctggc gaaatctcgt
    44761 ccccaatctt tttttgaaaa gctagtcctt ttcccataca aacgacttct tccccgaatc
    44821 catttttcga aatgacgaca ttgttattca aaattttatc aattttcatt ttgcttcgcc
    44881 ctctcaagaa aaataaaaaa acctagaatt cgcctactgt ttcttagctg aattctggtt
    44941 ttgcctgccg tacagtaaca atccatttct tattcaaaat catcatacca tcgaattttt
    45001 atttatgcaa gccttttcat ataaaaaata aaaaaactgg aacacaagtc tatctagaaa
    45061 acaaccaaat cagagatgca cctctttgcg tttctctttt tttcagcaaa ttggtttttc
    45121 cttatcgatt catatccttt ggctgttttt aatctgtaac gacttatgtc ccagtatcct
    45181 aaaatgtttt tgtctctaga ttgaaagcag atctcatatc atctgtcgca tcttttatcg
    45241 atgtaacact ttccagttct ccttgtacaa ctagtcgcgt gacggcgtcc atgtcgccac
    45301 aagtaattag tgtgacaatt tcttttccct cgacttcatt cagtaattgg gtatctgtag
    45361 gagcaacttt ttctttaaaa aatgttttgt atgtatacac attttctaaa tctgtcaaat
    45421 agatcttggc acccatctct aaattttcta gcggtgtaaa cagcaattca gggttcgttg
    45481 cacgatggct tgctaaggca taatttcctt ttcccatttc ttgatctgga gataatgttc
    45541 ctgctccgta aagtagtgac gtattatcca agcctttgaa aaccggtaaa ttgattgaaa
    45601 ctgaagggac tgcgatacta gcaatcactg gtagatctgt tcctctcatt tgcgacctca
    45661 tgaccccttc tgtcgtcatt ggttctaccg catcaaagtc aaaactgaca tctttatcgt
    45721 tattcttttt cagatcttct tttgttacat tcgctatagc atatttatct cctgtctctc
    45781 tgacaaaata atccttgatc tgttcattga aaatcaaagc tagtcctact aataacaaga
    45841 ggaacaagag gatattgact agccatcttt ttgcttttcc tttttttttc attttctctc
    45901 cctccagact cattttactg tttttctttt gctctttcca acagccgaat gacacatttc
    45961 tacaactctg tgtatttttt tcgtacacct gcgctttttt ctacaggata tttcaaatta
    46021 tttttgatca atttttcttc aatgatctca tctagatcca ggtgaaggtt gtctgccatc
    46081 atgtatgaat agatcaatac atctgccagt tcttctttga ttcgttctaa ttgtgtttct
    46141 tttacttctt ccggcgtttt ccattgaaaa agttctaaca attcagatgc ttccaaagaa
    46201 atagagatag ccaaatcttt ttcgttatga aattgtcgcc aattacgttc atctctaaac
    46261 tggttgactt tttccatact attcatttgc ttgccttctt ccaatcactt cttacttgat
    46321 taatcatacc atttttttac ataaaaaata gatgatgctt ttaaaaaaac tcctgttttc
    46381 ttttatttaa aataaatgtc atagaaaatc aataatttag gtagccacca tgtgtatgca
    46441 gtagcgtgtc ttcttccctt gaggcttttt gtttttcgat acgttcttct tcccaaacag
    46501 acaacgctac tagaatctct cttaatgatt ttccttcttc ggtgagcgag tactctactt
    46561 taggcgggac ttggttatag gcttttcgat ggatactttc atcttgttct aactctctta
    46621 actgctgggt cagcattttt tttgtgatcg tagggatttg cttgcttctc cacgcctaaa
    46681 ataagcgata ttgctcttcc ctttatctgg tgtaagcaaa gcagttagca ttttgacgat
    46741 cgtgggttcc gcttttcact tcttatctac ttagtatcat gaagtatact ttcgtactaa
    46801 tactaagtag cggaagtgtc aactactttg tttaactaca tagcaaaaga aacagaaggg
    46861 tgacaacggt catcacgctt ctgtttcttt tgaatatgct gtgttgttgt ctctatctcc
    46921 gtgtagactt tcctcaaact tatcctttga cagtaagtca gtaggctgat ttgctctttc
    46981 tagatgccag atacatttct acaaaaaaat agtgattctg ttttgaaaat tcgattttta
    47041 gttctgcatt ttcgtaaaca tccaacattt ttttcgcact gtatagacct aacccgccat
    47101 tttttttaga ggtttttcct cttttgacca tctccgatag cgtcaaattc gttggatttg
    47161 ccttgttcgc tatactaaaa cgtgatccct ctttttcttc cagcagattg atcgtaatcg
    47221 gactctgatc ggctacacga tgatcgaatg cgttagaaat aaaaatagat aaacagcgaa
    47281 taaaaacaac cagatcaatc gggattgttt tcacttcgac aggaataact acttggatcg
    47341 ggattttctc cgattctgct ttttctagaa attttaaaaa gacacccctg acagctgggg
    47401 atacaatatt tgttagctgt tgtatttgcg gtttttcaaa aatagctgca gaatgatctt
    47461 ctaatatttc taaaaattcc aacgtttctt ttttattttc ctgaagagta atttttaatg
    47521 aaaacagtag acttttataa tcatgtttga attcctctag atgatggtaa tattcttcat
    47581 ttatttgata cagttgaagc gattcctgat gttgtctttc ttttacttgg aaactgatca
    47641 acataatgga aaaaaacaaa gaaaaaaaac tgatgactat cgacaaaaga gatagaagca
    47701 gataattaga tgagcggaag tgaacacttc ccaataccaa gtaaatcacg ccccagctta
    47761 ataaaataca aatacctcca aaatagtgtt cagttggata agtaaaaagc aagttgagaa
    47821 atctcgtctg tttgtctata ctttttacta atagatagaa aataaatgcg ataagtagtg
    47881 caaaaaaact tgctgttcca agatgtctct ttcctaaata atccatcaga tcaaaaacca
    47941 gaaaaaagct gagtccagtg atagcaaaaa aaagattact gatcaaacag ctgataaaca
    48001 ggtttttcgt agtaaaataa aaaagaaaca tcaataataa gccaactagg taaggaaagc
    48061 tcactttagg gataggaagt aaaagtgtca ccaccatcaa agctatacaa ccaaagctgt
    48121 tttttactag cagttgtttc gaaaagaata aatagatacc taaatctaaa atcaaagtaa
    48181 aggtcaaata aagtgtcact gttgcgacca tcttattcct ccattgtttt gtttcaaaat
    48241 gtacacatat ttttttaata ggagcctgca tatgaaaaag tattttatca tagaagataa
    48301 ccagtttcac agcgaatgga tcatcagaga attggtcaat catcatatcg agaatatcgt
    48361 gcagctcact tctatctctc aaatcaatca gttgatccag ttgggcacta ttgataatca
    48421 cgatgttttc tttatcgata tcgacttgaa gcaatcactc acaggtattg atgtcgccga
    48481 acaaatacgt aaaaaaaatg cacatagtaa tattgttttt ttcaccgctt atacgaattt
    48541 tgcagtagag atcatcaatc ggaaaatcct tccgattgga tatatcagaa aagacaggaa
    48601 tttatctgaa caaattcgtc aagtgctaga acagatcaat tggaaagaac agcagatcgt
    48661 aaatgtcgat aaaatcttta ttgatcaaaa aggtggaagt ctcgtcctgt atcctcggga
    48721 aattttatat attgaaagta tcaaaggttt gaaaaaccaa gttatggtca aaacaactac
    48781 agaagataaa ttagtgaata cttctttaaa acaattgaag acagagttag aaaagtatgc
    48841 ttacttttta cttttaaaat cttactgtat caacttatcg caattgcaga aaattgattc
    48901 tgctcattat gtgttagaat ttttcaacgg tgactcctta ctcgttggtc gaaagatctt
    48961 tgaaaagaca aaggaacgtt tccatgactt ccaaaaaact gcttcgagtt gatgaataag
    49021 agctgagacg taagcatagc agatttcagg gagtgggcct tgaatcggct ccactccctg
    49081 aaatcgttga tatgatttta cgcttcctct tctgtaggag catctgttaa cgaccatgtg
    49141 aatgttgaaa cgtagttgtc tacagcataa ttggatacag gaacaaataa ttccactgat
    49201 tgtctccctt gttccacatt ctctcctaat gaataaatcc atgtgccaat cccttcattt
    49261 gctcctgcag ttactagatc tactgcctga tctgctggga tttcgattcc ttcagcgcca
    49321 gttccagaaa cggtagatgg cattccactg ctattagttg cgctatttcg aatcacggcg
    49381 tttttgaatt tcacctttga accagttagt ttattcgtat cagatgttgt accaataaat
    49441 tctgttcgtt tgactgataa tttccaaccg gcataatttc cgcttttatc cgttacttgt
    49501 acgaaattct gtacttgtgt ttgtgctcca gtactgtccg ttccgctttg taatgcagca
    49561 tatactgttt ttgctgtacc taacttgatt gttccaaaat caagatctgg agcttggtca
    49621 atcgtcagta atccggatgt tccctgattt ggttcgtcag gtgtaaaagg agtatcagga
    49681 tcatttggat caacaggagg aactggaaca acgtcttcag gatttgcagc atctggtttg
    49741 gtcacagaaa tcgttccttc tgtagtggct gagctcttat tctcagcaaa aacactttgt
    49801 gtacataata atagacttcc agctagaacg gtacttaatg caattgtttt tttcatttct
    49861 ttttccttct ttcttttaaa ataaataatc cagcaaataa gccaataaga atgactccaa
    49921 caagtatcag ccatcgccag tcaaatgttt ctggctgatt ttttaaatca atatcctttt
    49981 cattcaattg gtccgctgca tcatcagtta tttcaaaatc ggtatggaga ttccagcgtt
    50041 tttctttcca agcacccttc acaaccgctt gataatgtcc cggagctaat ttttttcctt
    50101 ctaatgggac aagtaaatca aaatttgtat tgggtgctac atctattttt tcttttttta
    50161 ttgaaaggta aggctctttt gtttcttttt tgtagatttc aaccgatgta gtcaagccga
    50221 acatataaga agcatttgga ttttgaagat tcgccttaat cacatttctt ccatcgcttt
    50281 gatccgcata agcttttttt aatttcaaat ctggggaaga ctctttttcg ctatttcaaa
    50341 tcaagattgc tttcacttga gaaaactgat tgacgacacc aactgtactt ttgttagaaa
    50401 cgttctcttt tttttcttga aacgtgatcc ctcctgcgac cacaccagcc agctcttttt
    50461 ctggcgcttt cagtgaaagt gtctcagtga ccgcactttg aggaggaaca gttatttctc
    50521 ccactgtttt tgctaagttt ttcatatcat aacttaatgt ttcatcaaat ttactttttg
    50581 attctccata ttcgatcaca ccgtttgaat tcgtcgtagc ggagtggatc aatgtctcaa
    50641 ctgttactga tttatctgtt cggttaaaga ccgtgtaggg gatttcctgt gttttcccag
    50701 gctccatcct tacatttata tatgttttgt tcttatcaat ctgattagcc ggtaattcaa
    50761 aatctaccgc aaaattgaat tcggaggcat ggacaggtgt ctgccaaagg atgaatccaa
    50821 ggagaaggat cataagtaaa gagcagttgt ttcgatgact tttattccat attcgattca
    50881 tcaatcatcc tccttgatct ttattctgtc ggcgcatctg ctaatgtcca tgttatctct
    50941 gctgagtaat taccagctat ctgattttct ttggttaaga caagtttaaa cgcccgaccg
    51001 ttatttaaat attctgaata atctgcttgc acattcgttg aattttgtcc tctgacaatt
    51061 tccagttctt cttcattcac taattgatac tcctctcctt tcttgaaata taggacatcc
    51121 tgcatagaaa atcctgcatc atttgtgaaa ggctgagata attttatcgt taagatccac
    51181 ggatttttcg tttctcgctg atcataaatc cataattttt cctgtatatc catttggcca
    51241 aatgcttctt gtgtcaaacc agttgctttg atctgaccaa aatcgagttt ggaaggggca
    51301 agaaatgaga gaccaccttc tactacgatc ttcgtagcag gtgcaaaagg cggggcatca
    51361 tgataaacaa ctggcgtatc acttggattg tgattcccaa tttcatcgtt ggtacttggc
    51421 ttgttctgca cacccttagc agtagcttct cctgcttggt cgtttaatac aatttctaat
    51481 acatctcctg caaaaagttg attttctggg atagaaagaa caaattcccc tgtatcatca
    51541 atagttgttt ctgacaaaac agcttgattt tttttcaatg aaaccaaggc tcctgcttca
    51601 cctgtacctg tgatcgtgcg agaagctgca ttgatcaatg tggtacttaa attagcaggt
    51661 tcaggaggaa tcacatcgac tgtcgttttg gcagagtcca gttctgtcac ttcttggcca
    51721 cctcttactt ttttagcagc aacaacttgc acttgatcct ccgtttttaa tctctcatca
    51781 ttgggtactt gaacttcgaa caagccgcct tgttcgcctt ctccccatgg gttatatcct
    51841 gccccttcat tttcatctgt ttgtccttgc gtgagagcag taatttctac agatgatttc
    51901 gtcgtatctg cgtaagtaat acgtaaagtc acttctactt cgcctgtcca agcatctctt
    51961 ggctttgcct cgacaccttc gggaacaacg acatgcccat agattttttt atctgcattc
    52021 gttggcactc ttaaggattc aatcatcggg agctgattat tggcactgat tcgattataa
    52081 tttttcattc cggcacctgt ctgagcaaat tcatttgtta aacctgcact agggcttgat
    52141 acgaaattgc ctaagtcggt cccattgatc gataagtctg tcatatcaga aaaataatcc
    52201 gggtcttttt caaagttatc gaatgttgcg cctgtttttc gccataaagc gatttctgaa
    52261 tgggcgatct ttaaagaaga atctccattt ccttggaata cccagccgcc tatgccattg
    52321 gtctcgcttc caatccgttt gttttggaaa tcaaaatagg taggagattc taagtccaca
    52381 ttcaacacat ccccactgat gatgccggtc ttatcttccc ctgttttccc gaccatgact
    52441 aaataagttt tttcacctgc cgtaaaattt aaatcaatat ctccagttgt atctacagtt
    52501 gctccggaat ctgcgcgtat atcgatattt gatttttctc cagtgactgt aaaatcagct
    52561 gaacctggac tttctagaaa accagaagcg tcataaaatt ggatcgcttg atttccttga
    52621 ttgagtctgt tcacgtaaga tttgccatca cctgtatttc ttaccctcaa tgagcctcca
    52681 ttggttactg tcagcttttg cgtaccattc tcaaaacgaa ctgcgctgga acgcccactc
    52741 tgcttatcga tgtcgataac tgatccggct ccttctacga tcaaagaaag gccttttcct
    52801 acaaaacgca tcccattgtt cccgggttga tcgtcatctc cagccatttt gaccgagatt
    52861 tgtccaccac ttgatacatg gatttgacta gaatctcctt gcaagcgaaa tccgcccgta
    52921 gttgctgtat tgatggtgat tttcccacca ttcgatacag tgactttcga tcctgaacct
    52981 ctcatttgga tgattccgcg attgttgtca ttggtaatat tgatctttgt atttaccgaa
    53041 aattcgcttc ccgaaccact gacttcaaac atgacattac caggtagcgt atcgactgcc
    53101 tctccttcat cgctaacgcc atctgcccga tcataatcaa gagagacctt tgaaccgctt
    53161 aagatatgga ttcctggcgc tgtttttcca ctaccagtct catctcctga atctagaaaa
    53221 ccgtagaatt tccctagata cggtccataa agttgactac cattgatcac attcaattga
    53281 tagcttctac aaaaataggc tttacccgga gcggcattca gtccttcctt cactggttgg
    53341 acatccaaca caccggaaac accatctaat gtgacagttg cacgaggagc gtaaatcagt
    53401 cctaatttgg acccatcata caaatcaagt gtcccggtaa attttatttc tcccgtaaaa
    53461 tgatatctgc ttccccaaaa atcggtcata taagcaatga taatggcatt tccatcgata
    53521 tttggtgtca atccacctgg atgtgcagct gttcctttaa aattctggaa ctcgatcgac
    53581 tgaggtatct cattgatata aatatggtgt gtgccgaaat tgtgagcgaa tccaccaaaa
    53641 tcaataacga tatctcgttt gatctctaat tccctgtcta aagtcaaagg ctgaatcagt
    53701 cggatcttag ttactgattc gtctgcgaga agctgcgcca gttcttctgt tgtggcagct
    53761 tcgctgtacc catccgctga aacagctctt gccagtgccg cttcactgct atcagacgtt
    53821 tgttcagcta cttctgaggt ctcacttgtc ttattttctt cttgcctttt attagtagaa
    53881 gcctcacttt gagattctgt agtttcagaa gtaatcgatt cttctgaaaa agtagtcttg
    53941 tcaaaacttt gattctctga ttgcgttgat tcgtttgttt tctctacatc ggcaaaaacg
    54001 gatgtcacca tcaagagagg gaagacaaac aaaatcgacg caatcagctt ttttacacat
    54061 tgattcattc tccaccatcc tctctgacgt tttgacaatt ttattttatc gttttaatct
    54121 atttccaatt gtactttcat atttaaaata tgtattttta tggaaaaatt tttctattag
    54181 gtgtaaattt cttttttgta caaaaaaaag gctgtgacaa aagtcttgtc acagtccctt
    54241 atcccgaaca agcggtgttc tcccgccaac tcctcggtca caacctccat tttaacaata
    54301 aacctgtaaa tgtgagggca atactttgat tgtcacaggt aatttatccc cttcatctcc
    54361 atcaacattc gtcgtcaatt cttcctcatc agctaatgac acaactaatt gctcacaagt
    54421 aaggtaggct agatttttcg tagattcatc tactcctttt aataggtctg gcaatgtttt
    54481 aagcgtatcg atcaaagaag aatcttttaa ataaaccaaa tggagcttac catcatccac
    54541 ttttgcattt ggaagtaatg tttcaaaacc gccgactgag ttagttagtc ctatcagcaa
    54601 ggtactgctc tcaatttctt ccttctttcc atcaacttct acgtgaaacg agtaggattg
    54661 tgtggaagct aactgtttga ttcctgacat gaaataagct agttttccaa atttcgtctt
    54721 cttctccgca tctacatcat tgattgattc agggatagag ccaattgcga cgacattcat
    54781 aaaatagtcg tcatttattt ttccaatatc caatggcttg actgattcaa tcgaaaaatg
    54841 attgatcgct tcctcaggtt ctagcggtat ccctaatgct cgagccaagt cattcaccgt
    54901 acccaatggg aaaaatccga aatttggccg atgttcttgt tcagcgattc cactgattcc
    54961 ttcgttgact gttccatctc cgcccatgac aaacacacta tgataccctt ccgtagccgc
    55021 ttcgcgagta aagttttttg catcccctgc tttttttgta tgcaagacga ccacttcatc
    55081 aaacaccgat tccaattttg ctatcgctaa ttgctcaaat tcctttgctt gttcaccgcc
    55141 tgaactgggg tttaccacta ataaaacttt tttcatcact ttctttccct tctgattgat
    55201 ttctttaata taacgatagc ataaagaaaa aaattaggct gtgatgaagt actgttcatg
    55261 aaacattcat ttattttttg acaagataaa acatccatac tatgaaactg aggaaaatta
    55321 aaacaatgat ataggaaata aaagaaaatg ataggcattg tatacctaat aaagtaaaaa
    55381 tcttaggtgg cagataaaaa cacaaaaata gcaagagaat gtaatagcgt ttcttccact
    55441 ttttgttatc agaccttata gcgatcagag gaataccaaa tgcataactg aaaaactctt
    55501 gatgtactgt taatatgtca cggccttgaa taataaatgc gacaatcccc gctataatta
    55561 gtgtgcctaa caccaaaacg gaaaattgat gaatatattt tatatacaaa tgatccattg
    55621 tacttcctgc ctttttattt gtgagaaaaa caaatccgta tacgtgaata acttctagaa
    55681 ataaaagaaa tttgcaaaac cactccattt atctacttct acgtgcattt tttgatattg
    55741 ttttctttat ttaagattat acagaaaaat atctagggtc gctggattct aagtaaaaaa
    55801 aatttttaaa ataaaaagaa ctaaatcaag tagtaattaa actactgttg atttagttct
    55861 aataaaaaga tttagatgaa cagttttatt gatttaaata gttcatattt tttatttata
    55921 ccgcttattt actacttgct gtactagaat ctgaagtgaa tgtactcaaa acattttcaa
    55981 agtctttgtc tttgattttt acattagcgt ctttcaatac ttcgccaatt actttattct
    56041 ggaaggtact gtcacttaat tttgtgtctg ttgcaatttc tttcaattct tttttgtatt
    56101 tatccatgtc gttgcctttg ttttggtttt tcaccatttt aaccacataa tattccgtag
    56161 tataagttga agcattggta gaagtgatca catcagaaac ttgaccatct ttcaacttaa
    56221 aggctgcttc tttgacttct gccggaaccg ttgtagtagt ggaatcaaat ttcacttttc
    56281 cgccatcttc ttttgtcgta gtatcagttg acttgtcttt ggctaattta gagaaatcgt
    56341 ctcccttatc agcagctttc ttcacatctt ttgcatcatc ttcacttgct acttggataa
    56401 tttgagcttc cacttctgga tggaaagatt cccaagccgt ttttaaatct tcatcggtca
    56461 atttgatatg tttctttaga ccttcttgaa aggctagact ttgtttgata ctgtctttga
    56521 atgtttgttc tgtgtaacct gcagatttca attgtgaatc aaatgaatca cccaattgtt
    56581 tctttgtttg atcatattga gaatcgattt ctttggttga tacgtcatct ccatatttat
    56641 cttcaaatac ttgataaacg atcaactgtt gcacagcgga atctgctcct tgcatttttt
    56701 tcaagctatc cattacttct tgttcgctga ttgtaccgcc tttataacta acggctgttt
    56761 tttcaccggc attcgtacag cctgacaata agccagtgcc tagtacgatg gcaagtgcta
    56821 aaaatctttt tttcatttat caattctcct acttttcgtt ttttgcgaat tgctaaaaac
    56881 aaaggtgttc aagatgaaaa tcatcaagaa cacctttact aatctgctcg atacgtcctg
    56941 aatagatata aggctacaca atccagaaag ttcgctcagc agaataaatg tcaagtagca
    57001 catcacacct taatatacta cttcttttgt taccgttttt caagtctatc ttgcgttccg
    57061 tatttaacgt aactaattct tagaacaaat gttatcgatt ttgtttttct tcttccgctg
    57121 ctgggatatt caaatcagac acttttatgt tcgattttcc agacattttt ttgttgtttt
    57181 tattatgata tatttttata caactaaaaa tgaatatatt aaaactacca aaaataactt
    57241 ttaacgctgt ttcattatat gttttttcta ttttatataa acttttggat acttaatacc
    57301 aaccggttgg attgtaccaa catgagaatg tgtcagttct atttcctgtc gcacttgcat
    57361 aggcccatac tccttttgtt gcatttgctg atcgaataga gtttccaact ttcgcttttg
    57421 caccgtgacg attatagaca cgactatagt ggttagaatg acctgttttt ctcgtacaag
    57481 ttgtatcact ataataaacc catcttccag agctatgatt aattgctgtt ctagaaattt
    57541 cccggtctac tggtaaattt gacttaataa tattaccttt agcatcaaaa tgaatctctc
    57601 ctggctttaa agatttgtaa tagatttcac cgtgaactga atttgcttcc acttcagatg
    57661 acatagcgtt tcctccaaca aaacctatcc ctagtgcaca aataataact gcttttttca
    57721 taaaacgaca tcctttcctt tttgattttt aaagcatgat cttcttgttt cctattttgg
    57781 tcagatatgg atcatgcgtg actacgataa ctaacgtacc ttgggaagct tgttgcgata
    57841 agatttcaac tactttatcc ttatttcttt tatccaatga cccagtcggt tcatcggcta
    57901 aaatgatttt gctattcttc aataagactc tcgcacaggc gattctttgc ttctcaccac
    57961 cagataatgt gtagataggc gtacttagct tcttgtttat atttactttt ttcaatgcct
    58021 catactttgc tgttcgctta tctgaaacat ttaaattcga aaacttcaat ccaatatcta
    58081 aattaaaatc gattgtttca tattcaatca acccaaaatc ttgaaaaaga aaagatatgg
    58141 tatttctttt tatcaagcgc ttctctctcc cagttaattg tttcgtctct tgatcgaaca
    58201 aatagaattg tcctgaactg aactgatcat tcaatcctaa tatattcaat aacgtacttt
    58261 tacctgctcc actttctcca acaacagtca caagatcccc agcatccgca gtgaaacgat
    58321 ccaaatgaag aatcgttttg tttcgaatga tttttttcgc accttttatc actatcaacg
    58381 gctccaattg ttaacctcca ttcatcagca ataactgact tttcttttct gtagacaaaa
    58441 taaaaacaac tgaaagaata aaatcaatga agctaaagaa aaaaatatta aaaagatatt
    58501 taggctctct ttctataaga aaaaggaaga ttacacttgt cgttccccat ttgatgaggt
    58561 tcatcatgaa gaaatcttta tacgtttcaa agagcgaata accacttgaa cgaaagatag
    58621 cgattttccg acaattgtag acaaaatata tgcataacat gacatagctc aacaagaaga
    58681 aagaaaagaa agtgagtaaa aatatctgta tcaagtaatg aaatgatctg cttaacactt
    58741 tgacagctat ctcttcagtc tgagtatacg gaaatgataa ctgcaagtta tcacgtaact
    58801 gactttcttc aatcagctca aataaccttt ctttcgacaa agagcctatc ttgatcttta
    58861 acggtggata gatttctccg ctgagaatat ttctttccaa atacatactg tttttcaatg
    58921 tcagtatttc aacaaccgga taatgttcga tccaggggat agaaggaata aatgtataaa
    58981 tgggttgatt gttttttata aatagaaatt tcggttcttt ttcactgatt tcttgataat
    59041 attctgttat ttgcgccagg tcatttttta aatctgtatg tctatatcgt tcagggatca
    59101 atataacagg gtgcgtttct ttttcggtta tttggatcaa ccttccagag acatcaatca
    59161 aaggaaattt cttaagataa ttcagattta tttttatccc tctgccaaac tgttcatttt
    59221 cttcattcaa gtaatctgat acattgacta atatacttcc gtttttattc aaagtttcat
    59281 ataattcctg atctgctgct gatgcaaaac gattggaata aataaaatcc acgtggtttt
    59341 tcccgatggt tactggataa aatacatgat agtcctcacc agcgatttct tttggtggtt
    59401 catgggaaac agctaactgt gaagaagaaa aaacgatagt tgctaattcg acattggcaa
    59461 cggtcaaaag aagagaaaac aacaatccaa atgtaaaaag aatgtattgg atatagttga
    59521 acgcttctct taacgaagcg attttttcaa ctaataaaag agaaatcaaa gagataccat
    59581 atattccgct gaacaagaaa acgtacatct ggaacaaata aagaaattgc tccttataat
    59641 acataaaaat aaaaccacca atgattcctg caattatgaa tgtaataatg attttctttt
    59701 tccccaataa acaccacaat acttgatgta atgaaaagtc attattcaac aataaactga
    59761 tttctttatt catcaataag aaaaaggatg ataacaaaag gaataaaaga atcaatccaa
    59821 tagttaaata cactcgataa ttatatacgt ctgtatttga aataaccgaa tacgaatagc
    59881 tattttcaaa gtcttcaaaa atattagtgg cataatcagt gtgaaatgct tgattgaatc
    59941 gatcagtgaa tagtgaaatg aaccgttcat aggcttcttt ttctctcgtc atcaagaaaa
    60001 attcaccttc aaaatctttt attttgatcg attcttcaat ggacgttacg cgaaaggagg
    60061 tattagaata gggtaccttc tcgtgagaag atttgacatc cccttgataa tacaatgtca
    60121 tttcaagctt tggctgtatc ttgaagttcc aagatgtatc tacttcattg gattcattta
    60181 ctgttttttt catataagaa agctcagttt ctgatgagac attttcaaat accatgatta
    60241 gctgctcata ttcttcttgc ccaataaaat gttgtggcaa tcgaatcgga gtataagtat
    60301 ctgtttgatg gaaaaattca tttgtccgat agagtacctg tttatttata ctaaccaagg
    60361 caaaaccact catgataccg gtcactatga ttgtgagtaa aactgtgaac ttttttttga
    60421 ttatcaccac tctcccttac ttaaatgatt ctttgataaa atcataccgt ttttcatcaa
    60481 aaaaacgtat ctatcaagtg tataggtcat gtagaataaa tctatatact gtagaatcga
    60541 aaggagaaga agatgaacac cggagaaaaa atacgaactt accgcagaaa ttgtaagctt
    60601 acgcaaaaag aattaggcga aaaaattggt gtttccgata aaacgatttc tagttgggaa
    60661 aattctcgta caatgccaga ccttgaaatg ctgtcacttt tacatcatac tttaggtctg
    60721 ccgattgatt ttccttctat tgaatcagct accgaatcaa tagaaccaac attgccagat
    60781 gaacctcttg ttaacaagaa atcttattca tcaaaacaac ccttctcact caaaaagaca
    60841 ctatctttct ttctgctcgt cttttgtttg gtctatcttt ttcttatcaa ttatcaatac
    60901 aaaaatgaaa cgatcgatcg attcaatcct tttttgaaac cacaaatcgg ctataccaca
    60961 ctccctgtag gatacgtgga caattccatc gaacgttggg tatgtaatga tatctttggc
    61021 catggtacat ggctgtcttt tcacgctgga gaggtagaaa aagagaaaaa ccatgctgta
    61081 atcaaacaca aaggaaaaca cgtcatgaac gttcgcctgc tcacttggga agccattccc
    61141 tcagattacc aagaaatcct cagccaagac tatgacgacg ccttaaaaag aagccctcag
    61201 gagccagata ggcattaagt aggagatgaa cgtagagata gaaaaaatca atgcacatga
    61261 tcttatccaa aaaacagggg tacaaattaa ttgaaaaagc cttgagatga aagttcctaa
    61321 ataaaaaagt tcagaatgat aggtatttct ggcttatagt ataatattta cgccacagcc
    61381 tcccaatgat tctttttgga atcataatca aggatggaca ttcacaaaaa tattttgctc
    61441 attcgatccg aatagattaa cgaccaatct agtaagtaat atagattggt acgtagtcaa
    61501 gctgcacata atttagatca attttagttc ttttttatat ttctatgatc tgctcattta
    61561 cttttatatg atggcttaca gtaacataaa taaatatgat aatcgctggt agtttatcca
    61621 agagtactca ataaaataca aaatggattg ttcaagcgcc tatcataatg ataaaatgaa
    61681 acaaataaac gatacaagga ggaagagact ttgagtaatg gcagaacaat tttttcattg
    61741 taaaaattgt tcagttgtta atctatggga tttcggtagc atgggcattt ttttatagag
    61801 gaatcacacc aacctggccg ttttatctct tgttagtggg aatattagta ggaaattttg
    61861 cccctcttca ctatcgtacg aacttttttc caactgattc tggccttttt cttaaaacaa
    61921 agacgcgaaa attagaaaac attattgaaa tgacaattac ctgtataatc attttcttag
    61981 ttatattcct aaacgtcttc taataagaat ctcttaaaat tagcttgtta cagttagtta
    62041 cctagtttat ataacacagt tatcttatct tccttatcaa catcttttgc caactctttg
    62101 tcttaaattc gaaacatata agcagagcta cgaacaccaa gactgtttcg gctctgtttt
    62161 ttgcatcaaa ttcactctta tatagctgat aatctcggct ctttaaaaga aattattttc
    62221 gcttcatttt attataaaaa ggctaggaac cttgatgtta tcaggttcct agcctttatt
    62281 taagtattac cccacagacc cttccatata gatttttaaa ttacacacta gtttaaacta
    62341 aaacaaacaa ggaaacactt accatttaat cataaaatta gctcatatga atatattaga
    62401 atgtaaaata gactggtcaa aatctggtca aaaaagagga ctaatacagg tagtcctcag
    62461 actgtagaca aagtccattt tatggatgat
//
LOCUS       DAZBTF010000009        56527 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000009 DAZBTF010000000
VERSION     DAZBTF010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.009"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..371
                     /locus_tag="ACHGNS_000614"
     CDS             3..371
                     /locus_tag="ACHGNS_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFN6936591.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            551..1492
                     /locus_tag="ACHGNS_000615"
     CDS             551..1492
                     /locus_tag="ACHGNS_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFN6936592.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1561..2292
                     /locus_tag="ACHGNS_000616"
     CDS             1561..2292
                     /locus_tag="ACHGNS_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFN6936593.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2436..4616
                     /locus_tag="ACHGNS_000617"
     CDS             2436..4616
                     /locus_tag="ACHGNS_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFN6936594.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4636..5118
                     /locus_tag="ACHGNS_000618"
     CDS             4636..5118
                     /locus_tag="ACHGNS_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFN6936595.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5134..5895
                     /locus_tag="ACHGNS_000619"
     CDS             5134..5895
                     /locus_tag="ACHGNS_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFN6936596.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6026..7360
                     /gene="murC"
                     /locus_tag="ACHGNS_000620"
     CDS             6026..7360
                     /gene="murC"
                     /locus_tag="ACHGNS_000620"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFN6936597.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7667..8152
                     /locus_tag="ACHGNS_000621"
     CDS             7667..8152
                     /locus_tag="ACHGNS_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFN6936598.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8145..8828
                     /locus_tag="ACHGNS_000622"
     CDS             8145..8828
                     /locus_tag="ACHGNS_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFN6936599.1"
                     /translation="MNNPSTLMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8954..11599
                     /gene="polA"
                     /locus_tag="ACHGNS_000623"
     CDS             8954..11599
                     /gene="polA"
                     /locus_tag="ACHGNS_000623"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFN6936600.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11644..12480
                     /gene="mutM"
                     /locus_tag="ACHGNS_000624"
     CDS             11644..12480
                     /gene="mutM"
                     /locus_tag="ACHGNS_000624"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFN6936601.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12444..13073
                     /gene="coaE"
                     /locus_tag="ACHGNS_000625"
     CDS             12444..13073
                     /gene="coaE"
                     /locus_tag="ACHGNS_000625"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFN6936602.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            13125..13457
                     /locus_tag="ACHGNS_000626"
     CDS             13125..13457
                     /locus_tag="ACHGNS_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFN6936603.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13769..14260
                     /gene="nrdR"
                     /locus_tag="ACHGNS_000627"
     CDS             13769..14260
                     /gene="nrdR"
                     /locus_tag="ACHGNS_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFN6936604.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14283..15656
                     /locus_tag="ACHGNS_000628"
     CDS             14283..15656
                     /locus_tag="ACHGNS_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFN6936605.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15656..16582
                     /gene="dnaI"
                     /locus_tag="ACHGNS_000629"
     CDS             15656..16582
                     /gene="dnaI"
                     /locus_tag="ACHGNS_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFN6936606.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16711..17151
                     /locus_tag="ACHGNS_000630"
     CDS             16711..17151
                     /locus_tag="ACHGNS_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFN6936607.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17295..17996
                     /locus_tag="ACHGNS_000631"
     CDS             17295..17996
                     /locus_tag="ACHGNS_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFN6936608.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17993..19114
                     /locus_tag="ACHGNS_000632"
     CDS             17993..19114
                     /locus_tag="ACHGNS_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFN6936609.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19129..20361
                     /gene="pepT"
                     /locus_tag="ACHGNS_000633"
     CDS             19129..20361
                     /gene="pepT"
                     /locus_tag="ACHGNS_000633"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFN6936610.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20493..21401
                     /locus_tag="ACHGNS_000634"
     CDS             20493..21401
                     /locus_tag="ACHGNS_000634"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFN6936611.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21436..21708
                     /locus_tag="ACHGNS_000635"
     CDS             21436..21708
                     /locus_tag="ACHGNS_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFN6936612.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21719..22765
                     /locus_tag="ACHGNS_000636"
     CDS             21719..22765
                     /locus_tag="ACHGNS_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFN6936613.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            23016..25625
                     /gene="clpB"
                     /locus_tag="ACHGNS_000637"
     CDS             23016..25625
                     /gene="clpB"
                     /locus_tag="ACHGNS_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFN6936614.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(25776..26462)
                     /locus_tag="ACHGNS_000638"
     CDS             complement(25776..26462)
                     /locus_tag="ACHGNS_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936615.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(26440..26934)
                     /locus_tag="ACHGNS_000639"
     CDS             complement(26440..26934)
                     /locus_tag="ACHGNS_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFN6936616.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(26954..28174)
                     /locus_tag="ACHGNS_000640"
     CDS             complement(26954..28174)
                     /locus_tag="ACHGNS_000640"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6936617.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28321..30156
                     /gene="lepA"
                     /locus_tag="ACHGNS_000641"
     CDS             28321..30156
                     /gene="lepA"
                     /locus_tag="ACHGNS_000641"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFN6936618.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            30352..31263
                     /locus_tag="ACHGNS_000642"
     CDS             30352..31263
                     /locus_tag="ACHGNS_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6936619.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            32274..32510
                     /locus_tag="ACHGNS_000643"
     CDS             32274..32510
                     /locus_tag="ACHGNS_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936620.1"
                     /translation="MFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTELKDKN
                     NVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            32623..32865
                     /locus_tag="ACHGNS_000644"
                     /pseudo
     CDS             32623..32865
                     /locus_tag="ACHGNS_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            32955..33194
                     /locus_tag="ACHGNS_000645"
     CDS             32955..33194
                     /locus_tag="ACHGNS_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936621.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            33341..37624
                     /locus_tag="ACHGNS_000646"
     CDS             33341..37624
                     /locus_tag="ACHGNS_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFN6936622.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            37723..38088
                     /locus_tag="ACHGNS_000647"
     CDS             37723..38088
                     /locus_tag="ACHGNS_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936623.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            38090..38542
                     /locus_tag="ACHGNS_000648"
     CDS             38090..38542
                     /locus_tag="ACHGNS_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936624.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            38991..40466
                     /locus_tag="ACHGNS_000649"
     CDS             38991..40466
                     /locus_tag="ACHGNS_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFN6936625.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            40489..40800
                     /locus_tag="ACHGNS_000650"
     CDS             40489..40800
                     /locus_tag="ACHGNS_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936626.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            41160..42416
                     /locus_tag="ACHGNS_000651"
     CDS             41160..42416
                     /locus_tag="ACHGNS_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFN6936627.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            42413..42958
                     /locus_tag="ACHGNS_000652"
     CDS             42413..42958
                     /locus_tag="ACHGNS_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936628.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            42959..43459
                     /locus_tag="ACHGNS_000653"
     CDS             42959..43459
                     /locus_tag="ACHGNS_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936629.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            43818..44096
                     /locus_tag="ACHGNS_000654"
     CDS             43818..44096
                     /locus_tag="ACHGNS_000654"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936630.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVLQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            44116..44460
                     /locus_tag="ACHGNS_000655"
     CDS             44116..44460
                     /locus_tag="ACHGNS_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936631.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            44479..45390
                     /locus_tag="ACHGNS_000656"
     CDS             44479..45390
                     /locus_tag="ACHGNS_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFN6936632.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVSAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            45469..46461
                     /locus_tag="ACHGNS_000657"
     CDS             45469..46461
                     /locus_tag="ACHGNS_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFN6936633.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            46458..46676
                     /locus_tag="ACHGNS_000658"
     CDS             46458..46676
                     /locus_tag="ACHGNS_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFN6936634.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            46689..47099
                     /locus_tag="ACHGNS_000659"
     CDS             46689..47099
                     /locus_tag="ACHGNS_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFN6936635.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            47151..49679
                     /locus_tag="ACHGNS_000660"
     CDS             47151..49679
                     /locus_tag="ACHGNS_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN6936636.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            49695..51905
                     /locus_tag="ACHGNS_000661"
     CDS             49695..51905
                     /locus_tag="ACHGNS_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFN6936637.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            51913..53004
                     /locus_tag="ACHGNS_000662"
     CDS             51913..53004
                     /locus_tag="ACHGNS_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFN6936638.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            53025..53567
                     /locus_tag="ACHGNS_000663"
     CDS             53025..53567
                     /locus_tag="ACHGNS_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFN6936639.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VGQEAQKIDAIKIYYVRSAY"
     gene            53762..54019
                     /locus_tag="ACHGNS_000664"
     CDS             53762..54019
                     /locus_tag="ACHGNS_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003657401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936640.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            54033..54365
                     /locus_tag="ACHGNS_000665"
     CDS             54033..54365
                     /locus_tag="ACHGNS_000665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936641.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            54410..54958
                     /locus_tag="ACHGNS_000666"
     CDS             54410..54958
                     /locus_tag="ACHGNS_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFN6936642.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            54965..55417
                     /locus_tag="ACHGNS_000667"
     CDS             54965..55417
                     /locus_tag="ACHGNS_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936643.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            55514..55855
                     /locus_tag="ACHGNS_000668"
     CDS             55514..55855
                     /locus_tag="ACHGNS_000668"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936644.1"
                     /translation="MSIDDCLEEQIRLFLKFLKVKVQNNYFTCYSYAHPVLNSLPFNS
                     LVQGLITNIVLLVCTPNEIFVKKIGSGISSVRLKEKEYTQKIIHIPKPEIKRFEIKDY
                     SALGLFSIGYW"
     gene            56223..56414
                     /locus_tag="ACHGNS_000669"
                     /pseudo
     CDS             56223..56414
                     /locus_tag="ACHGNS_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT        19258 a         9128 c        12102 g        16039 t
ORIGIN      
        1 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
       61 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      121 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      181 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      241 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      301 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      361 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      421 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      481 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      541 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      601 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
      661 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
      721 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
      781 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
      841 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
      901 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
      961 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1021 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1081 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1141 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1201 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1261 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1321 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1381 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1441 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1501 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1561 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1621 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     1681 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     1741 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     1801 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     1861 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     1921 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     1981 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2041 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2101 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2161 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2221 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2281 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2341 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2401 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2461 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2521 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2581 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     2641 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     2701 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     2761 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     2821 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     2881 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     2941 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3001 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3061 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3121 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3181 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3241 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3301 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3361 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3421 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3481 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3541 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3601 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     3661 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     3721 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     3781 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     3841 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     3901 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     3961 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4021 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4081 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4141 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4201 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4261 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4321 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4381 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4441 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4501 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4561 tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4621 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     4681 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     4741 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     4801 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     4861 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     4921 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     4981 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5041 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5101 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5161 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5221 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5281 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5341 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5401 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5461 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5521 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5581 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     5641 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     5701 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     5761 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     5821 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     5881 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     5941 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6001 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6061 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6121 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6181 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6241 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6301 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6361 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6421 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6481 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6541 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6601 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     6661 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     6721 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     6781 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     6841 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     6901 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     6961 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7021 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7081 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7141 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7201 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7261 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7321 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7381 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7441 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7501 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7561 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7621 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     7681 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     7741 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     7801 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     7861 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     7921 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     7981 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8041 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8101 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8161 cattaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8221 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8281 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8341 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8401 cctactctct tttgaatggc ataacattag cggttactct tgcatactac gatattggaa
     8461 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8521 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8581 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     8641 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     8701 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     8761 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     8821 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     8881 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     8941 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9001 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9061 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9121 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9181 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9241 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9301 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9361 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9421 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9481 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9541 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9601 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
     9661 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
     9721 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
     9781 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
     9841 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
     9901 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
     9961 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10021 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10081 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10141 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10201 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10261 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10321 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10381 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10441 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10501 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10561 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10621 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    10681 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    10741 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    10801 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    10861 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    10921 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    10981 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11041 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11101 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11161 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11221 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11281 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11341 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11401 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11461 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11521 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11581 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    11641 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    11701 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    11761 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    11821 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    11881 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    11941 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12001 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12061 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12121 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12181 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12241 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12301 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12361 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12421 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12481 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12541 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12601 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    12661 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    12721 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    12781 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    12841 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    12901 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    12961 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13021 aaatacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13081 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13141 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13201 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13261 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13321 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13381 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13441 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13501 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13561 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13621 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    13681 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    13741 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    13801 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    13861 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    13921 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    13981 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14041 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14101 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14161 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14221 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14281 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14341 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14401 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14461 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14521 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14581 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    14641 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    14701 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    14761 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    14821 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    14881 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    14941 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15001 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15061 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15121 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15181 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15241 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15301 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15361 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15421 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15481 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15541 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15601 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    15661 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    15721 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    15781 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    15841 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    15901 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    15961 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16021 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16081 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16141 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16201 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16261 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16321 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16381 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16441 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16501 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16561 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16621 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    16681 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    16741 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    16801 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    16861 atatttgtcg gaacgattga aagtatcact caagtagaag cagaagaaga aacgcttcag
    16921 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    16981 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17041 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17101 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17161 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17221 tatgggaatt tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta
    17281 gaaggagtga agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact
    17341 ttgtccctga atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg
    17401 cattgatatt aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt
    17461 atgaatcagc acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc
    17521 ttgcaaatgg tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg
    17581 gtatgggagg ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa
    17641 gcggacaaga acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc
    17701 tgcaagaaaa tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat
    17761 atgaaatcat tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga
    17821 tgttcgggcc atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag
    17881 agcttcaaca aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac
    17941 gtcaaaaaca aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat
    18001 cagcggacgc actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga
    18061 aggagaccca gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat
    18121 gacattagac gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct
    18181 gattgctaaa catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca
    18241 agaaaaaatg tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa
    18301 catggatatt atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt
    18361 ggagagttat ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc
    18421 tgtggatcat gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg
    18481 tgattatacc ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg
    18541 tgcacaacca gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga
    18601 ggttattttg ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc
    18661 ttatgaggaa cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt
    18721 aggacgagta ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa
    18781 agaagcgttc caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt
    18841 gaagcgcatt gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca
    18901 aagagcagat gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag
    18961 cgctgggttg attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt
    19021 tatcgagaaa tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc
    19081 tgaaacaaat acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat
    19141 cttttgccac gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa
    19201 acgactccat cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa
    19261 gagttaggct tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta
    19321 ccaagcaatg ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca
    19381 gattttaatg cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg
    19441 atcgtactag atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa
    19501 aattatgaag gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac
    19561 aaatcaggga ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc
    19621 cctcacggta ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat
    19681 aaattcgatg ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta
    19741 ggtgagttgc agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa
    19801 aatgtacatc cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat
    19861 tttcataatc agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc
    19921 ttccatttga tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc
    19981 gatcatgatc gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag
    20041 ttgaatcagc gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac
    20101 atgaaagaaa tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc
    20161 gagcttggta tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc
    20221 tcttacatgg gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga
    20281 ttcgaatttg tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca
    20341 gaattgaaca caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca
    20401 atgatggaat gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc
    20461 ctcttttttg aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca
    20521 aaagttcagg gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag
    20581 aaaagcagcc agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg
    20641 aattagtcaa gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg
    20701 gcacgattca tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat
    20761 tacttccagg tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag
    20821 aagaagctgc tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca
    20881 atgatgaagt cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga
    20941 tcagtcaaga agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt
    21001 tattgctgaa gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag
    21061 gaaaatccca actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg
    21121 acgactcagc gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata
    21181 ttttccgatt tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg
    21241 gcattactta tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca
    21301 ctcgaacgga tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag
    21361 gattaacagt ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa
    21421 gttggaagaa gagtaatgga tgtattgtta cacaatcctt tgtggtttta ccttttcatt
    21481 gtgaattttt acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc
    21541 tggcgtgttc ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga
    21601 ttgatctctc gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc
    21661 gctgcaggtg tacttgttga tttgattctc atttattttt tccattaagg agcaggagat
    21721 gtcgtctaaa ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt
    21781 tgccattatt tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc
    21841 taccagtgtt gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga
    21901 agggcgctca atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg
    21961 gttcttgacc tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct
    22021 gatctcggag atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt
    22081 gacagcactc catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt
    22141 ggtcatccaa ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc
    22201 tggtatcctt ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa
    22261 tgttcaaata ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa
    22321 atgggtggat acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat
    22381 ccaagacagg actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg
    22441 gtatgtactt ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac
    22501 catttcgttt atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat
    22561 tggttccttt gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc
    22621 agtttcttcg cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt
    22681 tgtgtatgtg attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa
    22741 agagaataaa gaaaatcaag ggtaggtctt ttataagatc tacccttttt atcaaatcaa
    22801 agaaaaacgt tatggataag ataattttac gtttgtttgg ctcttttttt atatacatac
    22861 aatcaatttt gtttttttaa gatttatata agtcgagaaa aaaagtcaaa aaactatgtg
    22921 tatattttga ccaaaattga ccaaagcgat ataatagttt ttgtaaagat caaagaaata
    22981 agtgatctta cctttaatta ggagggtata cctgtatgaa tattgagaaa atgactacga
    23041 cgcttcaaga agcgattgct gaagctcaac agattgcggt cactcgaaaa catcaagata
    23101 tcgacattgc ccatgtatgg aaaatctttt tacaatctaa tcattttggt cgaaatttct
    23161 atacggatgc cggattggat gtcgaatctt ttgaacatga aattgatcgt ctactggatg
    23221 agtatccagt tgtttctggt ggaaacgtcc aatatggaca aaacctcagc cagaatttct
    23281 ttcgattatt gaatgaagca gaccaaatac gtgaaagctt cggcgacgag tttctttcaa
    23341 cggaagtcgt tattttagcg ttgatgaaat taaaaaatta tccgttaact gtttatttga
    23401 ataaaaacgg attatctgaa aaagaattgc gtaaaaacat cgaagagatg agaggaggag
    23461 aaagagtgac ctcacaaaac caagaagaac aatacaaagc attagaaaaa tacggcgttg
    23521 atttagtaca gcaagtcaaa agcggaaaaa tggatccaat catcggtcgt gatgaagaaa
    23581 tccgtgatgt catccgtatt ctttcacgaa aaacaaaaaa taatcctgtt ctgataggag
    23641 aacccggtgt agggaaaacg gcaatcgttg aagggcttgc tcaacgtatc gtacgcaaag
    23701 atgtgcctga aaatttaaaa gataaaacga ttttttcatt agatatgggt gcattgatcg
    23761 ctggagcaaa attccgtggt gaatttgaag aaagactaaa agcagtcttg aaagaagtca
    23821 aaaaaagtga tggacgtatc ctcttgttca tcgatgaaat ccacaatatc gtaggggcag
    23881 gaaaaacaga aggaagtatg gatgccggaa atctgttgaa accaatgctt gctcgcggtg
    23941 aattacattt gatcggtgcc acaactttag atgagtaccg tcaatatatg gaaaaagaca
    24001 aagcgttgga acgtcgcttt caaaaggtat tagtcaaaga accaacagtg gaagatacga
    24061 tcagtattct gcgtggatta aaagaacgtt ttgagatcca ccatggtgtt aacatccatg
    24121 acaacgcatt agtagcagca gctactttat ctgaccgtta tattactgat cggttcttac
    24181 cggacaaagc aatcgatttg atcgatgaag ccagtgcaac gattcgggta gaaatgaact
    24241 ctatgccaac ggaattagat caagttacta gacgtttgat gcagttggaa atcgaagaag
    24301 ctgcactgaa aaaagaatca gatgatgcaa gtaaaaaacg cttgaaaaat ttgcaagaag
    24361 aattagctga acttcgtgaa gaagcaaatg cgatgaagat gcagtgggaa acagaaaaag
    24421 aagaagtcaa ttctgtttca gcaaaacgtg ctgaaatcga taaagcaaaa cacgaattag
    24481 aagatgcaga aaataactat gatttagaac gcgctgctgt attgcgtcat ggtacgatac
    24541 ctcaattaga aaaagaattg aaggaattag aagctaaagc aaaagacagc gagattaaga
    24601 tggttcaaga gtccgttacc gaaaatgaga ttgctcaagt cgttggcaga ttaacgggta
    24661 tccccgtaac gaaacttgta gaaggtgaac gagaaaaact aattaaattg aatgaaacac
    24721 ttcataaacg agtgattggt caagatgaag cagttgatgc ggtcagtgac gcggtaattc
    24781 gttcacgggc tggattgcaa gacccaaatc gtcctctagg ttctttcctt ttcctaggac
    24841 caactggtgt cggaaaaacc gaattagcga aagcgttagc tgagaacttg tttgactcag
    24901 aagatcatat ggtacggatc gatatgagtg aatatatgga aaaacatgct gtgtctcgtc
    24961 tagtcggagc tcctccaggc tatgtaggtt acgaagaagg tggtcaatta acggaagctg
    25021 tcagacgaaa tccttataca atcgtcttgt tggatgaaat cgaaaaagcg catcccgatg
    25081 tcttcaatat cttgttacaa gtcctagatg acggacgctt gactgattca aaagggcgag
    25141 tagtagattt caaaaatacg gttctgatca tgaccagcaa tatcggttca cagttactgc
    25201 tggaaggcgt gactgctgat ggaacgattc cagaagcagt ggctgaacaa gtcaacacgc
    25261 tattacgtgg aaacttcaaa ccagaattct tgaaccgaat cgacgacacg attttattta
    25321 caccattgag cctagacaac gtgaaaggaa tcgttgacaa aatggtggcg caattggcac
    25381 aacgtctgga acatcaagaa atcctgctga caatcagtga tgaagccaaa acgtggattg
    25441 ctgaaaatgc ttatgaacca gcatatggtg caagaccatt gaaacgattt atcactaagg
    25501 aagtagaaac accactagct aaagaaatcg ttgcaggaca tgttatgcct aaatcaaaag
    25561 taacgattac tttacttgat ggacaacttc atttcaaaac agaagagcta gaagaaatcg
    25621 tctaatcttc cattgagact ataaaagaaa aaaggggtct gacacaacta gtgtcagacc
    25681 tccttttttc aaataaacag tgttccagaa gtagtttctt cgaaaaataa gccgaaattt
    25741 cttgaagctg cccgcttctg tcacaacctc ttttattagg tatttttcac tttttctaat
    25801 ttataacttt caaagtaacg ctcatctttt ttactttgag accatgcaaa caatcccatt
    25861 aaggcagcac ctatcgtatt tgtcagcaag tcgcccatcg tatccatcaa tgctgctcgt
    25921 ccgacaaaca gtgtgccatc agaagcagca aaacgttgaa gattcatgcc taagaactgg
    25981 tcgcataaga attcccagaa ctcccaaaac acgccgcaaa gtccagcaaa cgcaaagccg
    26041 aataagagga acaaccaagg actggttttt gatatctcag catctttcat caagtaacca
    26101 atcaatccat agccaagagc tgtcaggacc attggactca ccgcatgtaa gattttgtcc
    26161 caaaaagaga tgatactgat caaatgcatc cctgtgccta aaaagacaga gatgaacaag
    26221 aaaaaccagt aaaacaaaac gattgctgct ggtattttaa gtttgaaaac tttcaaaatg
    26281 acttgtggca tgaaaatcaa tgcaatccct aataaagatt ccacgataaa tgtgattcct
    26341 tgagaagtcg aatatttttg tgtgataaat tcataaaaat tatagcagac aacgaataca
    26401 ccaaaagcaa gtaaagctat ttgcaccttt ttgatttctt taatcactga aagtccctcc
    26461 atctaaaaat ctttttactt ctgaaagcaa ctcaatttct gaagaaacca cttttccgtt
    26521 caatttgatc aaacctattg tatataagtt caggtagtga aattgatttt cagctgtttc
    26581 ttgtagtgca gcgatttttt tcgggtgggt tccgccttgc tgtcgagtat cagtatataa
    26641 gccgatcact gggatgcctt tcgcataagc cacaccgatt tccgatgcta ccccggcatc
    26701 gatcgtcagc ccatctagta aagcaatcat taaatcacta gccaacactt tttctgtgtc
    26761 agcaagagca atcattcgac tgtctgcata agcagttttg tcattgattg cttcattttc
    26821 ttgaggaaga tagatcgaaa gctcagaaga gagctctctg atttctttaa ctaactgttg
    26881 gttatataac aaatcacttt tagcaaacat tggtgcggca aaataaattt tcatctaaac
    26941 atcatccttt ttatcaaaaa taatcgctaa agtaagcttc catgtttgga atgatacgct
    27001 ctagtgactt cagtgtttct tcatctgttt ctaaccgttc gattcttgcc aaataagaag
    27061 cacggcgata attcatcatc aagcaagtca atgtttggat atccagtcga acggattttc
    27121 ctataggttc atttaagaca cggacttgtc cctgttcatc ccatgttaaa gcgaagacac
    27181 cattattcca aggagcgaca ggatcttcaa tgatgaaatg aaaagcatca gctgttccaa
    27241 caaacggaaa ccgttgaagg aattctttta catcaacgat ccgagccata aaatatggtt
    27301 cgatctgctc tttgatctga ctatcttcaa tcaaaaatgc taaaggctca tttttataaa
    27361 tatctccatg tacccagtag atcatggaaa aatgtgcgga aataaagttc cataagccat
    27421 ttcgagcttc ttgatcaaga tagaacattt cttttacgtg aaatatttct tcagctaccc
    27481 agtagaacag aacacctctt ggttcatggt tgctatcgta gtagacagcg gctgtgcgct
    27541 catcttcatt ttcgaaacgc caatactctt cccaatgaaa atcactgcgg aataaagcac
    27601 catggttttg ttgggcaaat ttgtcataaa cggaaaatac gtctggatga tcaactggta
    27661 atcgctcaac cataccactt actgggattt gtttaggtag ctgtgtatct ctgattttaa
    27721 aggaaagttt atcagacatg atttcccatc ctttacgccg atagtagggg atactgtaag
    27781 gatacaaata agagatccat tgcttattct tgcgcatatt atccaatgca acgataatta
    27841 gatcttgcat caatccatgg ttagcatatt ctggataagt tccaactcca gtcacaccac
    27901 ccattttata acgtgcaccg tgaatattga cttcacaagg atagatagcg atttgagaga
    27961 tcagtttatt ttcgtggaac catccgaaca ctttcgatac ttccaaaatc ggttttttgg
    28021 aacggataaa agctcgttta ttctcaaacc cgctttcctc gatgtctgct tcagtcactt
    28081 ggaacacata ggaaagcaat tcattgaatt gatcgatatg ttcttgagtg accggtttta
    28141 attcaagatt ttctctaaaa tctttttgat ccattttttc atctccattt attagcttga
    28201 taacagtata gcaaaattct acaaaaaaaa cttctatagc tgactaatta aaaactcttt
    28261 aaattactgc ttttcgttgg tataatggaa agtgctgatt atatgaaaag aggagacgct
    28321 atggatataa agaaaatgaa agaacgccaa gagcagattc gtaatttttc gatcatcgct
    28381 catatcgacc atgggaaatc tacattggcc gatcgtattt tagaaatgac gcacactgtg
    28441 acgtctcgcg aaatgcaaga tcagctgtta gactcgatgg atttggaacg tgaacgtgga
    28501 atcacgatta aattaaatgc tgtagaactc aattacacag cgaaaaatgg agaaacatat
    28561 atctttcact tgattgacac cccagggcac gtcgacttca cctatgaagt ctctcgaagc
    28621 cttgctgcct gtgagggtgc cgttttagtg gtcgatgcag cacaaggaat cgaagcacaa
    28681 acattggcca atgtttattt agcgttagac aatgatttgg agatcctgcc agtcatcaat
    28741 aaaatcgacc tacccgcagc agatcccgaa cgtgtccgtc aagaaatcga agacgtaatc
    28801 ggaatcgatg ctagtgaagc tgtattagcc agtgccaaat cagggattgg gattgaagat
    28861 attctcgagc agatcgtgga atatgttcct gcaccaagtg gagacttaga tgcaccgcta
    28921 aaagctttga tctttgactc tatttatgat agttaccgcg gtgttgttct aaatgtccgg
    28981 atcacggacg gtatggtcaa acccggagac aaaatcaaac tgatgagtaa tggcaaaaca
    29041 tttgatgtta cagaggtcgg ggtcttctcg ccaaaagcag ttgctagaga ctttttgatg
    29101 gtcggtgatg tcggttatat cacagcaagt atcaaaactg tccaagatac acgagtaggg
    29161 gatacagtaa cgttagcaga caatcctgcc gctgaagctt taccaggtta ccgaaaaatg
    29221 aatccgatgg tttattgtgg tctttatcca attgatactt ctcgttacaa cgatttgcgg
    29281 gaagctttag aaaaactgca attgaatgat gctgctttac aattcgaacc agaaacctct
    29341 caagcattag gttttgggtt ccgctgtgga ttcctcggac ttcttcatat ggatgtcgtc
    29401 caagaacgct tggaacggga attcaatcta gaattgatca cgactgcacc atccgttatt
    29461 taccatgtta ataaaacgga tggtacgact gctacagtag ataacccggc agattttcct
    29521 gaacctgtaa cgattcagga tgtagaagaa ccattcgtca aagcacagat catggtccca
    29581 aatgattttg tcggtgcagt aatggaactt tctcaacgaa aacggggaga attcattacg
    29641 atggattatt tagatgatta ccgagtaaat gtcgtctata atattccgct ttctgaaatt
    29701 gtctttgact ttttcgacaa attgaaatca agcacgaaag gctatgcctc attggattat
    29761 gaaatgtctg gttatcaaaa aagcaagtta gtgaagatgg acatcttgct gaatggagaa
    29821 aaagtcgatg cattaagctt tatcgttcac cgagactttg cctatgaacg tggaaaagca
    29881 atcgttgaaa aactgaaaaa actaatccca cgccaacaat ttgaggtgcc aatccaagca
    29941 gctattggac aaaaaatcgt tgcccgttca gacatcaaag ccttgcgtaa aaacgtcttg
    30001 gctaaatgtt atggtggaga tgtttctcgt aaacgtaaac tcttagagaa acaaaaagaa
    30061 gggaagaagc ggatgaaaca aatcggctcc gtcgaagttc cgcaagaagc cttcatggcc
    30121 gttctgaaaa tggatgagca agatagtgtg aaataactta taatcaatcg aaaaacctat
    30181 taaataaacc tttttgagta gatgcaacac tgaggattat ctgtataaaa gattttaaac
    30241 tgtcatttgg ttatcacggc gatactaaaa ttaacaaaat tggaatattt ttcttgaaga
    30301 tttttagctg tcagtatatg atgaaagcat ctcataggag gtgctttttt catggttgat
    30361 aattcaagtg gaagggttct taaatctcta agaaaagaga aaaaactatc tcaaaaaaag
    30421 ttagctgatt tagcaggtat ttcacaaagt actttagtta aatatgaaaa agggtcaaga
    30481 aaaatcccta aagacgtaga taatactcta agcaagattc tgaatataga aactctaata
    30541 aaagatgagg aagataagat tgagattctc atcggtaaac taattgcata cagagatatg
    30601 aataagttac ttaacaaaga gttagctgat aatataggaa ttagtgaagt attactcagt
    30661 tatgtgttaa atagaaagag aaatccgtca aaagaaatgc aaaaaaaaat cgatattttt
    30721 ctattaagta atgaaaaaga aattttgaag gagattaatc gtgatagtga gatatttagt
    30781 ttatcaaaag acgataaaat tgttatggga aaaagaatac gagaagtccg aaagaaccgg
    30841 gaagagacgc ttgaaaaatt tggtaaaaac tttaccatct atacaggcaa aaatgttata
    30901 agtagatggg aaaaaggtat taatattcca gatattgaaa aactaatgaa tattgcctat
    30961 cttggtaagg tcactgttcc ttaccttatg tacggagaag attataagaa tatacttcca
    31021 aaagatgaaa gagttagcga ttttaaaaag ataaattctt ttagtatggg attaaggatg
    31081 aggaaaatta ggaaagatta ttatctagaa agggaggagt ttggaaagct tttttctcct
    31141 tcaatttcaa aatggtccat agataggtat gagaatggta gagatatacc caatactaat
    31201 cgaattatcc aatatgctta tataggaaat ctttctttag aatttttgat ttacgggata
    31261 taaaaatagt ttacgtcgat acttattaag ttatcgacgt tttttattat agaaaaaatt
    31321 tataaaagga ggtttcttga tatgataaga aacaaaaagg gtttgttgaa agttgcttta
    31381 ctggcagctt ttttctaagt aactttttga caagctcaat ggcaacacaa gcggaagaag
    31441 taatacaaga actaactgtt atgaataaca gctagataaa atgggctatg tatgttatcc
    31501 aacaatttag taatcactag aagcctagcg aaagttcgct tgataagtta tagatgagta
    31561 ttgatttgta gtgtttccaa atcaaaaggg tacagagcca catgaaatat gttgtcctct
    31621 gcagcgtcag gatgggaatc cataccatgg agattcggag atctgtgagg gaatacttac
    31681 gtggttaata tgtcagttgt gttggacgta gtaatggtca tagtgggtag attgactgaa
    31741 ttcttatatg agaaaatgaa accaacaaga agatagggtt atttatacag aattggggat
    31801 cttttctgga aaagtaatcg gataatgcga gattcgtacg gtctttttta aatttctcta
    31861 aaaaaaacgg catcgaatgg gttgggtgat tgataagcaa tgaatcatag actgatatag
    31921 aataaaaaat gattctattt atctttttac atctatcgtt ttttcaagta gccgcaaatt
    31981 atatcagcat tcaacaattt ttgtatacaa gttctagtaa aaggatctga agaaaaagtt
    32041 ggttgttgtg aagataaaaa aagaatcagg ttttactgag tttttcttca aaatttgtgt
    32101 tattaggtta ggacaaagat aagaagaagt atgaagattt aaaaaaggaa tgaggaaaag
    32161 atagtaattt ccgattattg gagttttttt atttataaag aagaagcgaa taagttgtga
    32221 ttcctagatt tcgcgcgtga ttaattaatg agcaaaaaat gcggaaagtt ttagtgttta
    32281 atacacgagg acaaagtttc ttaggtattg aaggcacacg tgtaacggtt gatgggtggt
    32341 gtgattgttt cgaggttgga aaacatgttg aacttatggc atttactgag ctaaaagata
    32401 agaacaacgt ggaatttttc gagagtgata ttggttggga cgatcatgaa gaagtttatg
    32461 gaaaatttat tttgcggaag ggaaattctt atacgaatgg ggaagcataa gtagcgattt
    32521 gttagagatc attgatagga ttgaaattgt cgggaatatt tatgagaatc tagagttatt
    32581 tgaggtgata gaatgagtga tggttttgaa caagtatcaa taattaaaaa tcaagttcgg
    32641 gagattctta gaaaaaaagg ctatttggta gatagttatt ttgaaggaga ttacgaaaca
    32701 tgggtaggtg tctatgctcg tccagagaat aaacctacct atttagatcc tacgacgtca
    32761 gaagatgcct atttacaaaa taggtatcga gtagatggtt ttaagcaaga ctttgcggag
    32821 tggtttgaat gggaaattga aaacggagaa gtaaaagaag aataagagta cttgttgtat
    32881 caaaataagc cagtagaaca accaaaccag tagctagtaa tgacaataat actgaggaat
    32941 cggatcatta tggtatgatt gatatttctc ttccagaaga tcttaagcca gatcctgtaa
    33001 caattggcaa tagtgtcatg atttttgatt cattagatga agctatgtca tatggttgga
    33061 aagaaattaa ggatgaaggt agtaattggt atggcatgag atttggtgat ggtgaagttg
    33121 ccaaaaaagc tggtattcca attgggaaat ggacagttga tttccacgaa gcaggttctt
    33181 tttttgttct gtaattatag tgggagggta aaatcattga tgaaatgtgt tcagataaga
    33241 gcgccagata aaaatagaag agacagttca cgtcgttatc acttttggta acggcgtttt
    33301 gttttattat ataaaattct caggaaaagg aggaattctt gtgtatgcaa aaacgaaaat
    33361 gaattggttg aaagcactcg cattggtggc tttttctcaa ttctgtttct tggtgggaac
    33421 tgctcaagca catgcagaag aaacgacggc tatacaacct acgatggaag ccattgccca
    33481 agtcgcttct gaagtgacag aagtccaacc agcagaagcc ggggtcccag ctggtgaagc
    33541 cactgttgaa caggcccctg tgacagaagc gccaacggtc aattccaaaa cgactctcga
    33601 tcagacgatt ggggaaacac aacacccagg gaatagtcct ggtacttcac aaccagttga
    33661 agaaactgcc tcaattacaa cccctgcttc agaaacgtcc actctagaag gagaaaaagc
    33721 tgccgaagca acgccttctg tgcagccaga gtcttctgag aaacaagcgg aagaaaagaa
    33781 acgaatagaa gaaacagtgg aacaaccagc ggctactgct gacaaagaag gaaaaaatga
    33841 ggaaaacgtt gaaaaaacaa tctcttcaac cgcagctagt caaaatcaac caacgaaacc
    33901 aaacaaagaa tctacggcta gtattgaaaa tcaaaagaaa aaagagcaga acattccaaa
    33961 aacaggtaca cctacactgt tagtaacagt accaaccaat cgttcggttc gtgttcgccg
    34021 tgcaaccaat gcagctgttt taggtggtcg gaaaattgat tggggcgaaa atattggtgt
    34081 tacttctagt ccgaacaatc taaacttcac tttaaattat atggttcgtt ttgagattaa
    34141 tggacaagtg gcgttttgta ttgagcctgg tgtctcagcc gcagaaggtc aagtttatac
    34201 agctactgct ttgaaaaact atttgaaaaa ttcaaatgta cggaaaaaaa caagcttgat
    34261 ctcttattta ggatatgtca caaataaaga caaatccgat gaacaataca ttgcggcaca
    34321 atttatgatt tgggaagctt tgggcacaaa aatcccaatc aaaaatatcc gtgttgatta
    34381 tgatcgtcga aaagcagaaa ttcaacgttt gattaataac tttggtgaaa aagcaacttt
    34441 taataatcaa acacatacgg taaaaattgg cgaaacgttg aaagtaaaga ataaaaatga
    34501 ttttttgaaa tatgtgaaag acattcaaaa accaaaaggt gtcaatgtat ctattcacgg
    34561 caatgaagta tggattacag tcacaaaaga ggcaccggat aaagcaaaaa ttcaattgaa
    34621 taaaattaaa gttcaaggtg cgccattagc ctatcgtcgt ccaggagctc aaacaattgg
    34681 tgtgttacat ccaacagaac ctaatgcgtc tgttattaat ttacaaattc taaaacaagg
    34741 aaacattcgt gtgtttaaag aagatgcgga aactggtgtg aaagcacaag gtgcagcttc
    34801 tttagaaaat gcggtctatg gtttgtatca agcagatggg aaaaagatta aagaaattac
    34861 cttgaaaaaa attaacggca aagtacaggc agaaattaaa gatcttgatc ctggcaaata
    34921 tgttttaaaa gaaatcaaag cgccaaaagg ttatgtgctt tctgatgaag caattgaaat
    34981 tgttgttgaa cctggaaaaa ctgtcaatgc gaccgcaaaa gatcaagtga tcaaaggaac
    35041 cattgattta gtgaaagttg ctaataaaga tttagtcgat tcaacgaatc cagataacaa
    35101 accaaaatta gctggcattg aaatcacttt gacgtcaaaa acaactggca aagtggtgaa
    35161 aaccgttgtg acagacaatg atggttatgc tacttttggt aaaaataccg ttgtctttga
    35221 tacgtacatt ctttctgaaa ccaaaggaaa agaagggtat aaactatttg aaccacttga
    35281 agtcacaatt tctgaaaatg ggcaaacttt ccattatgtt ttagaagata aagtaattga
    35341 acaacgtctt aaagtggtga aagtggatca agaaacagga aaagtgattc cacttgctaa
    35401 tacagaattc aagatttttg atactttagc caatcgttat gtcacaatgg agattccaaa
    35461 tgatacagaa acaacggata tctttaaaac caatgaaaaa ggctttttcc taactaacgg
    35521 tacacttact tatggaaaag atcgctatcg tttggaagaa atcaaagccc ccgtaaatta
    35581 cgttttgaat caaaacccat tagtgttctc gattaccaat gatacagaac cgatcaaagt
    35641 gatccatttt gcgaaccgtc aagcacggaa aagtgtaaaa ttattcaagt atgaagaatg
    35701 gaacaagaag aaaacgccgc tcgcaggtgt gaactttacc ttgtataacg gtaaaaatca
    35761 agcaattggt gaatatacca ctgatcaaaa cggggaaatt ttagtgaaag gtctagttgc
    35821 cgatgactat tacttcttgg aaaatgcccc attagaaagt ttccaaccaa atacagataa
    35881 acaagcattc acggttgcat tcaaacaaaa tgcgccagat gatggggaaa cgttagtggt
    35941 ttctgtacgt aattacttga ttccaccaaa actagaaaca caagcgtcaa ataaagccga
    36001 tggtgaaaaa gtggttgatc cattagaaac gatcgtcatt aaagacagtg tttcttatac
    36061 caacttgttt gtcggaaaag aatacaccat ttcaggagta ttgatgaaca aggcaacgaa
    36121 tgcgccaatt ttacaagatg gcaaagaaat cacaagtacc ttggccttta ttgcagacaa
    36181 agtcaatggc acaaaagacc ttgaatttgt ggttaatgct agtgttctga aaggtcaaac
    36241 gatcgttgtt tttgaaaaac ttttccgtga tggcattgaa gttgcagcac atgcggatat
    36301 caacgatgaa gcccaaagtg tgaaagtgaa taatccaacc gttcatacca cggctgtctt
    36361 tgaagacgga ttgacagtcg cagacccaac aggattgttt accttaaacg ataccgttga
    36421 aattaccgag attcttccag gaaaagcata cttggtcaaa gggatcttaa tggataaagc
    36481 aaccggtgaa cccttgcttg tgcatggaaa tacggtagaa acgagtgttg gctttattgc
    36541 caaagcagaa aatgaaacgg tgatcgtacc atttaccttt gatttgacag gtataaatgg
    36601 tcgtgaaatc gtcgtgtttg aaagtctttt tagaaatgat gaagaactag caaaccataa
    36661 agacatcaac gacaaagggc aaacggtacg agtaactaac ccaagtgtac gtacaacggc
    36721 tacacataaa cccactggct tgaaagaggt taatccatta acgaaagtca ccattacaga
    36781 cgctgtgaaa taccacgatt tggtcgttgg aaaaacatac acagtcaacg gtgttctaat
    36841 ggacaaagta acggaaaaac cagtgctagt agatggaaaa gaagtcacaa gtaccttaac
    36901 gtttgtggca gaaacaacgg atggtacagt caatctagac tttattctag acgcacgtgc
    36961 gttgcgaggc aaagaagtcg tggtctttga agatctgttt agagaaggcg tattattggc
    37021 ttctcacgca gatattaccg acaatgacca aaccatcaaa atcagtaatc caaccattac
    37081 gacgaaagcg acgaataaaa atggcggaaa agagatcttg gccttgccaa atcaaactgt
    37141 gcttgaatgg gtcaaagtag acggtcttgc gattggtcaa atctatcgct taattgttca
    37201 aggctactta acaccagacg gcacaccatt tgaaggcact catgcggaaa aagtctttgc
    37261 ggccgataaa gaaatgatgg aattcttatt tgaattctta gtggacggtc gcaatcttgc
    37321 aggaaaaggc ttgagttttg cggaatggct ccaagcgaaa acggaagatg agaaagaaac
    37381 gtttgaagaa gtggcgaaac acaatgtaga tctaacaaac aaagatcaaa cggttcaaat
    37441 tgtggaagcg ccaaaaccac cgcaaccagt gaaaccagaa caacgggtta aaccagctac
    37501 tgttgtacca atgttgccaa aaacaggtga acaaacaagt agtatactta tggttcttgg
    37561 attagtagta ttagtcattg ctttaggtat aacactttat aaagtacgta aagccaaaga
    37621 ttagtcatga ggaacaagcg gttaggattt ttaaccgctt gtttgattgt aatgtagaaa
    37681 tatttagaat ttattttggt ttttaagagg aggaaaaact ttatggcaga aaaagcattg
    37741 cgtatgaaga aagagttttt aggtcaagta tttttattgg gttcacggga acctaatcca
    37801 ccaaaggaaa ttaatggtgt gtctgggcga tatgttcaat tacaagcaga gtgttcgcga
    37861 gaagaacagc ctgctttttt tgtttatctc acagctgaag aggataaaag attacgatta
    37921 ccagaaaaag aggtgcgtat ttcatttgaa gaagcttatt ttgtttttcg taaagaacga
    37981 gtgaatagtc gtgaaacaaa tgatatggga caaacagagc gtcgaacaac tggctatcaa
    38041 gatgttggtg tctttttaac tggtgtaaaa attggggagg ttttgtaaga tgggattaaa
    38101 atttccaaat aattatgaca ttacagaagc attagatgaa acttgtttag gagccgtacg
    38161 ctttggcgca ttagaagagt ctgatcgtgt atttgataat gaaacaggcg attatacggg
    38221 agaaattcga tcacagcgag cacaattagt ttttggaaat ccagatgaac caaagattgc
    38281 acgacgagtt aaatttccag cagatgtaga tttgactggt tttaaacgac gtgatcctgt
    38341 gaaattaatt gatccagaat atcatgtggt atatattcca gcacaagatg gcaatcgtga
    38401 atattcagat ttagtgattt atgcgaaagg attagagaaa gtagaagctc caacaaataa
    38461 tcctaatatg aaacaatcac aagaaaaagg tttagatcaa aaacaaccaa gtgttgattc
    38521 taaggaaaaa gagaaaaaat aaggtttttt tgaaagaata ggaaggagtc ttcctattct
    38581 ttttatctaa gatagaagga aggggagatt ttgaattcag ataaaaccaa tcaaatactt
    38641 tgagagattg tgcttgttat caaaatatcc aacaattgat tgagtatttt ggagacaatc
    38701 caacgatttt tgtacaaaca aatgcatata tttgtgcggt tattgggcag tgtatgtttt
    38761 tacgtgatct tgttcgtttt tcattaccgt accagtggaa acaagatcat caggaagtga
    38821 tttggattcg tatagtacga atatgtaata tgtgtgcaca cccttataaa aatcgattaa
    38881 atctttttcg attatgtttt tttactaaat gtgatattcc tgtattagtt gctttttgtc
    38941 aaaagttttt agcacaggaa aagtaaaaac agaaaggagg aatgtgacaa ttgaaagctc
    39001 attttttcaa gtatcgtggt cggcgaattc gctatttcaa tcgttattta tggttttgct
    39061 attatggttt ctttttgttt ccatttttga ttctgttagg ttggtttagt tggaaagtat
    39121 tttatccacg tttaagtacg ttttcgaatt ggcaaatgta tcttattcct ggaataagta
    39181 ttgtgttgta tttgttgctg gttagtgggt taattttagg gttgatgcat tggtcgcgac
    39241 taaaagaagg atattttgcc tctgtttatt ggcgtcagtt acttgttcga atgttaattg
    39301 acaatcggtt tgtttataca aagaaacaag ttagtgagaa acaaacaagg gaaaaaatga
    39361 gattaccaaa agtttatttt tatcaaaaga aacaagaatt agtggtttct tttcctttag
    39421 atggtgggcg ttttcacgaa cgatttttaa aaatgggtca tttgttatct gaagtatttt
    39481 tgcgtgattt aattcgtgaa gaacgagaaa aagcccgaat caattatgta ttcctttcag
    39541 attctggtcg gattcctatt gatcaatgta tagcagaaaa tggacgtatt catttaatga
    39601 aaactttgga ttgggtatat gatcaatcac caaatatgtt gattgctggt gcaatcggtg
    39661 gtgggaaaac ctatttattg tattctttaa ttcaagcttg tttaagtgtt gggacagtag
    39721 atatttgcga tggaaaagcg gcagatttag cagcattagg tgacgtcgat gtattcaaag
    39781 gacatgtgtt ttacaaaata aatgaagaaa tgattatttg tttacgaaat gcattaaaag
    39841 aaatgaatca acggtatgcc tatatgaaaa ccatgaaaga tcctaaatat gaaccaggga
    39901 aaaattatgc ttattatggg ttaccaccgc attttatctt ttttgatgaa tgggcagctt
    39961 tttatggttc gttggatatg gaagcgagaa atagagtcga taaattgatt cagccattag
    40021 tattaaaggg tcgtcaagca gggatgtaca ttattttagc aatgcaacga ccagatgcag
    40081 aatattttcc aagtggtgtt cgggataatt taacattccg tatttctgtt ggtcgattgt
    40141 ctcccattgg ttatttaatg acttttggag aagattttaa aaataagcca ttttacaaca
    40201 aagatcaacc tggtcgtgga tatgcttttc caggatttga tgtacctgta gagttttttg
    40261 cgccatttgt ttacaaagat tatcacttta tcaaagaatt tgagaaattt actgaaatga
    40321 ttgtctatga ttttacagta aaagaggata gtcaagatca aacgatggga gatgataatc
    40381 agttgttaca agaacaacta gagcgccaaa tgtatttagc aaaagatcca aaaggaacaa
    40441 gtttttctga tgcgcatatt ttgtaatcaa ctaaggaggt gaagttaaat ggcagagagt
    40501 tgttattata gagtagctac agcaatacga atgattaatc cttctctttc ttccagaacg
    40561 ttttatgatt ggttgaatcg tattgaacaa gtaactgatt accgcttttt gcgcaaagaa
    40621 cgtgttttta caggaaaagt tattaatcaa gtattgctaa cgaaaaaaga tatagaacga
    40681 ttgactcgat tgtatcatta tcgagtagat ttagaagagg atttaacttt gtcaatttat
    40741 cgcgtttttt ctcctgaaaa atatagtgaa ataacaaaat tagatcattt aattttgtag
    40801 ttattatcta tcgggagggc tctagacgta gtctggtagc cgcactctgc ctggcggtgc
    40861 gcattaaggc tgcgaagcat gggcctataa tgcgcactcg ctagcagcgt gtgcagctca
    40921 caaagaggtc gtgcgcttgc gcacggcctc tttgtgtaaa gccccccgat atataatatg
    40981 gggggcagaa ataacataac acatagaaaa aagttttata aacccttttg tatcaagtga
    41041 ttatctaagg tcagcagttg tactactttt cccaaaaaac gagggggact agttgatata
    41101 tcggtgtttc taaagggtta aaaaaatcga ttttgttatt ttataaaagg agcgagaaaa
    41161 tgacaccgat tgagctaaaa agctacagaa agaagttaag tgcaattcat aatataagtg
    41221 gagataaaaa gccgctatca caagcaagat ttgcgcaatt gatgggctgc tcaaaagatt
    41281 acattagaaa gatggaacaa gggaaaagaa aaatcccaga ggattttgaa caacgaatca
    41341 aggaacgctt tcgattgtat atgaaaagcg gaatagatct tcgagcaatg attgattatt
    41401 tgcgtctttc cttttttggg acagactaca aaacagtgat tcaacgtgta ttacgaatgg
    41461 atctgacaga atttgaaaca cgtccaacgg gcttgtatct ttttgactgt ctttctgttc
    41521 gtgggaatat ctgggtgttc tggcattcaa aacgaactgt aacagaaaat gtacttgtgc
    41581 aattttcagg acaaggttgc cgggaatatg aggaagtcat gcgtgaacaa caaacagatt
    41641 ggcgaacatg gctatattcc atttggaaaa atcaaggaaa gttggaagct ggttatacac
    41701 gtgtgcaatg tagccgattc gatattgcgt tagatgaact ttggggattg gaaacggaag
    41761 aacatttcga tctctttgaa ttactagaaa aacaaaaagc aggtttatta gaaatggact
    41821 ttaagagatt ccagaatatt ggcggttgtt ttttagatga tggctatttt cgaagtgaag
    41881 gattatcttt gtattttgga tcgcgaaaga gtcctctctt ttttaatttt tatgagaaac
    41941 gttttgaaat tgcgaaccga gaaaaaattt cagaattaga agcattaacg aaatatggta
    42001 tttataatcg atatgagtta cggttagcca atgagaaagc aacacaagca gtagaagcat
    42061 ttattttagg tgattcaccg aaacgtttag gagagattgg cgttggttta attaatgcaa
    42121 aacttattgt ttgggataga ctggaaagtg atgcacggat tgttgataaa cgatgggcaa
    42181 aattatttcg ttcgatggaa gaattggatt ttacagtaaa gccagaaaaa ttctcattag
    42241 atcgttcaga acgctggttt ttgaaacaag tagctccgac attaaagttg ttacaagaag
    42301 cagatcgtat tttaggaaga gactttgtac aagaagggat tgataatgcc gaactaaatg
    42361 acgagcaaca aaactatcta gaatggatgg gaacgtcagg agggaaaatt gcatgaatgc
    42421 atatacgaca acagaatggc tgcgcctgtt ggatttaaag gctgcaatcg aagcattaaa
    42481 tgaaaagatg gttgatttgt cttattttcg ttttcgagtt ccttatattg aacaagcggt
    42541 aaaagctgga cgttatcagg aaaaagagaa ctggcaagaa attgcacgct tattagaagt
    42601 tcgtaaggga tacgaacaag aactcgaaga gttggaattt agccgaagga aaggaaggct
    42661 agaattcatt cgcttttatc gattttcatt gccaatacct gcgattttag cggttaaaaa
    42721 aggctgcgat aaaatgaaga tatatgaaaa ctgtgtggca gcacttagca gcgaaaaacc
    42781 tcttatggaa gaaatttcat tggtgactgt cacttgggaa atgagtcgcc agccaacaga
    42841 agaacagaca tatctttctt tagaagagat tgagatagaa ttagaggaaa ttggtcgcta
    42901 tgccacttgt tctacctatt gtggatcggt cattagtatt gctggtgtga ttgtctaaat
    42961 gaaaaaaatt atgatttatg ttggtgcgta ttggtcacgt gatccaacag tattagaaaa
    43021 tccagaggcg gtacactatt gcttgcgcca attattctat ctgtataaag aacgattaga
    43081 atcgttgatt cgtcagttac catatactga cagacgttta gatgaacttc ttctaaggta
    43141 tccagcaatg tataaacgac gaaaaaatag attgttacca gaagagtatc ccattgaaaa
    43201 acatgaactt gaagggcgat ttgtggctta tttctatgat gatgttcgta tgcgcttagt
    43261 agaacaacga atggaaatag aaaatgatcg ctactatttt atcaattatt gtaagaagcg
    43321 taagtatcaa gtaactgatg atttttatga ttgtatttta caagatgggg aagtgattct
    43381 atccaaaatt gctccaagtt ttcgtgaact tgtgccgatt gattttttga aaaaatgtca
    43441 tttgcggatt cttccgtaaa aaagagctat aaaaggaaag aaaagtagaa gccaaatcgg
    43501 tttctgcttt tttatttttt ttaggaagga gaagatggta tgtcactgga atttttagga
    43561 gagccaattg aaaaggaaga gttcgtttcg cattatatgg atttgtttat tcaggtgata
    43621 aaaaactttg tgcgattgat gaattttatc cacgcgaaca agactatcta gccgctgaac
    43681 gaataaaaat cagtttatta tgggaaaaat tcacagatga aaataaccga ccgccagatt
    43741 atcgctattt atcagattgt ttaacgaata atgtgctttc taaagaatac ctatttcaca
    43801 aacgtgagaa aaatcaaatg tcggttgaac atttttgtga ttgttatatt gcattattgc
    43861 gtagtcaaca gttattaaca gtagaacgat atggtgcaga gtttggttat gtcttgcaag
    43921 ctgagcgtca tagagctaca agatgctatg aaaaagaaga tcaagtgatt gatggctatg
    43981 aagaattatg tattcgaaac aatcgctatt tgcaacaacg tttatatgaa caattgatga
    44041 tggatttttc aaatctctat agagggtatg aatgttcgat gattcaagaa aggtagaaga
    44101 aaagggggaa tttttatgtg gcgattatta tttttggtgg tatgtggtat ggcattgttc
    44161 tatgtgtatc gtgtctatct ctatccacga atggaacgat ggacaagttg gtatagagat
    44221 cctttaaaac aacgaaaagg attgaagcgg tatcgttatg aattacttgt tgaattttcg
    44281 gatgggcgac aagcacagta ttattttttt gcaaactatc caaaatacaa caccagtgaa
    44341 ctattgattc aattattcaa aaataatcca tatgttgggt tagaagaaaa cggacgggag
    44401 ttcttttatc gttcagataa tattagtgcg gcgtctatga acaaaacacg aattgcataa
    44461 aaaattggag gaataatcat gaaaaaaaca acattagcag tcgtaacaac actaggaatt
    44521 tcattatttt cgtttcaatt agggagttca ttggtttatg cagatgaaac tgtagaaaca
    44581 cctaagtcag aaattgtaga aacattgcca ggggaaggat taccagatgt tgagacagag
    44641 ctaaatggag gaacaacgga aaaaccagca gaacctgaac aattgcagtc agagaaacct
    44701 gaaaaagagc cagtgacacc agaaccagag tttcctgaat caactttacc gactgattcg
    44761 atcgaagaag aaggtgtgca tagttcaaca acaacggatt caagtatagt cacaactccg
    44821 gaacaatcag gaacaaccag ttcttctgaa aagaatactg attcgtcaag tacggaacag
    44881 ccaacgatgt cgtctaccag tgaagaacaa aaagaagaac cgaaacaacc tgttgaaaaa
    44941 tcagaagcaa ttccaaaaga aaataaacct attgtttcag caaaagaagt cacagtatcg
    45001 gtaaccccaa gcggtgaaat tacgacgaat acttctcaag gaatgagtgt accgattgtc
    45061 actagtaatg tagaggaatt gacccatatt ccaacaccaa ccaccccatt aaaagtagca
    45121 ggtgatcaaa caattgttgg tgtaaaagat ggtattccat taatccaaga tagtgaaggg
    45181 aatttggtaa aggatttgtc gattcctgta aagaaacttc caagtggcaa tattgaaatc
    45241 aaaacagcag atggaaaaac aaaagtctta ccaaagactg gtgaagaaat acatgttgca
    45301 ctttctgttc taggaggagt gttgacaagt attgctggat ttgttggata taaaagaaag
    45361 agaaaaggaa tagaaaatga gggtaattga atggactatt tttcagaaaa acaagcttat
    45421 cgttgtcaat cgtacgtact agtaaaaaca tactagaagg agtagcttat gaaaaaacga
    45481 caactacggc ggaaagaaaa agtttataca gagaaagtac cgaagcaaaa gaaaatccat
    45541 tcaaaaaaag cagtgaaatg gatttggtgt ttggttttat ttctttgttt ttcagggggc
    45601 ttggcatttt ttcaatccat gaaaacgaag gttcaagtgt cgtttttgaa agatgaaatc
    45661 caacaattac aaaagaaaga agtacaaagt cggtcagaat taacgtacac acctgaaatt
    45721 gaatccttta tgaatcgttt tgttgctttg tatatgaata cattcgataa tcatgaacaa
    45781 gaacaacaac gtcaagaaac attaaaaaaa gagttttatg cacaaaactt tattcaagaa
    45841 ccaggaagtg tttcaggtgt tcgaaaatta actgctgctt cgtttattgg attaaaagaa
    45901 attgaaacca ttccaactgc tagttataaa gtaacttatg atattcgctc gatgaatcca
    45961 gagagtaaga aagaaacaag tgtgacaatc acacaagtac tcaatcttcc cttttctatt
    46021 tcaaacaagg gctgtcgtat tattgcttat ccttatttta cggcactacc aaagaataca
    46081 gaaaaacaac gaatgattac ttatgatgac acattatacg aaacggttga tgcgtcggaa
    46141 gcgaaagatg ttgaaggatt tgtggaagat ttcttagata agtatgcgaa aaacaatgca
    46201 aaagatatgt catacatgat gaaagaacct gaaggactca atcaaacagc taaagttgat
    46261 caagtagaga tgaaagtatt gcgttataaa gaaacgaaaa aaccttttat tgtaaaagca
    46321 acggtcgtct ttcgagatgg tgatacgtca cttttacaaa aagaacaaat gacgattgtc
    46381 attacgaaaa aaacaaatca gtattatgtt gaaaaattaa cacataattg gttggatagg
    46441 aaaggagaag aacggaaatg acactgaagg gaattgtaga ttttatatta acgcaaggtg
    46501 gctatttttt aataatggtg gttgtttatc tgggattgcg aaattttatg aaatcaaaag
    46561 taggaaatgc attaatatct atcgcagcag gtgccgcagt ttatttcttc ttaaatgatc
    46621 caacacgtgt atttgatgcg tttggtaaaa tcattgaaaa attatttatg gattaggagg
    46681 atagtgaagt ggattgggaa ccttatgatt attcgattgt ttatgcggaa ccttatcgcg
    46741 tacaacagat tacaagcaat tggcgtcttc catttccagt tgcattagtc aatgcagcaa
    46801 cttttttgtt ggtcgtaatc tttgaatatt tatttttacg ttcatttatt ggttgggtca
    46861 tttctttttt tccaccagca ggagttgttt tttatatagg actcccctat ttatgtgtgt
    46921 tagcactggc aaaactaaaa cctgatggaa aaaaagtgca tttttattta tgggattggt
    46981 tttgttatat gattcgtatg atgaatggtg attgtgtgta ttataactgg gacaaggtat
    47041 caaacaatgc attggaagaa ttactttttg tgaataagta tgtttattta gaggattaga
    47101 aaaagggaca atcaactggt tgttcctttt ttattgggag gtgaaattca ttggtgcttg
    47161 taaaagaacc agtaaaagca ttcaagaaaa attgtttgtt aaatggagag gattgtatct
    47221 gggcctacta tcatgttttt ccacgaacaa tttcaaaaaa cacaccaaaa aagacacagg
    47281 aaataaaaga aatgatggct cgtttactgt tgcttttagc agattatgaa gatgtggagt
    47341 tggtgatgta tccacgaaca atgaatttac ggacgaagtt tgacacatta ggaaagggat
    47401 tttctcccga tagtcaagca attggtgcgt attatgcaaa agaaacagtg acattgttgg
    47461 aacgagaagg tcaagtctat cgtccttcat ttattttagg aatcaaagtc aaagaaaaag
    47521 caacggtgga gagttttcat gatgtaataa atctaatctg gttagattta gcaggaagtt
    47581 tagcacgctt atttggatta gaagtagatg atttggaaac tttgttttct gaaagtgatc
    47641 cttatgtaaa agaagtgaca caattacttt atagcttttc tattcgtccc ttagtaaaaa
    47701 aagagctgtt gtatctcaat cgtctgcaat atattcgagg aatggctcac aaagtagaag
    47761 aacaaaataa ttgggaagta ctgaaagaca atcaaggaac gtatgtacgt ataccgaagg
    47821 aacataaagg ccttcttcaa ttagaagatg agttagggag tagttattta gcattgttgc
    47881 ctattgggga aacacctgaa aatatgagaa attgtgatct atttcaattt gctcaggatc
    47941 tttctttccc agcagaattg cgaatgaaaa ttcactatcc acgcttgaaa ggacagggag
    48001 gtattggttt aaaactgaat tggctatcgg aacggttcaa aacagagcat aaggaattgg
    48061 ttcaaaatga tgaagaacct agtgaacgct tattaactgt tcgaggatta gtaaaacatt
    48121 taaagcaacg agtagataat gggcaacctg tcgtcgattg gatcgcatgt tttattgtat
    48181 acggtcaaac ggttgaagaa gttcgtcaaa atagtgcaac ggttattcaa caacttcata
    48241 cgattatcca tatccaccgt gcaaaaaatg atcaacgaga attgttctat aaatgtttat
    48301 tagggcaacc attgggacgg cagttacatt ggaagcaccg aacaactgta gaagcagttg
    48361 gcgaatggtt gttttcagca gtatcagaat taggtatgga atccggctgg tatattggtc
    48421 ggcaagatac attaggggat ggtggttctc gaggagaaaa aacaccatta gaagaattaa
    48481 tttatgcgtc aaatcgtttt gtctttttga atcttctagc cattgcggaa ggaatcaaag
    48541 gtgcgttgtt tgatgcgcct catgtagcag taactggaaa aaccggtaaa gggaagacct
    48601 ttttagttgg cttgctcttt ttgtatagtt cgtttatgaa tgtacaaagt ttatttgtgg
    48661 atcccaaagg agaaaagcgc aaatggttta cgaaactcat tcaagacccg tattatcaga
    48721 aacattaccc tttgtttgta gaacatttgc aaaaaatgag ttatgtcacg ttagatgcta
    48781 caaatccccg taattatggt gtattagatc caattgtttg tttatcccga gtagatgcga
    48841 aacaagtagc ccaggatatg attgatgagt taagtccgtt aggtaccaat caccttttaa
    48901 aaggggcggt attaaaggga atccaagaag ttttagttca aaaagaagcc ggacaacaag
    48961 ttggcttgat gcatgtggtc gactatttag aacaaatgga acaaaaagag gttcgagaat
    49021 atggtgagtt tttacgcttg acggtagaag attcgatttt acgtttagga ttttcttatg
    49081 gagaaaaccc gggattagac tttaatgcca aaacaacgat tttagagatc caagatttga
    49141 aattaccaaa tgacaatgtc cgaccagaac tatatacaga tgcggatcga aagtctttgt
    49201 gtttgatgat tagtttaggt cgtttttgtg aaatgtttgg aaaacgagat agtagtaaga
    49261 aaacagcgat ttattttaca gaagcatggg tatttaataa ttcgaacgca ggacgtagta
    49321 ttattgcagc aatggcacgt gtggggcgtt cccagatgaa tcaattagtg ttagatacac
    49381 aatttattgg tgacttaggt tcagataaag aaaaagggaa ttttggtgtc gtctttgctt
    49441 ttgatgaaga cagtgagcga gaaaagattt tgaatcattt acgtttggaa gtaaatgaag
    49501 cgaacttagc tgaaatgagc cacatgatta aaggacagtg ttggatgtta gatccctatg
    49561 gacgtgtagg aaaactcaat gtacacagtt tgtttgaaga aatgaccgct gcattacaaa
    49621 caacggaaaa aacaagtcac tctcaagtag aagaacagga tacgttaatc atgcaataga
    49681 aatgaggtga aacaatggct tatataaagc ataaggaaaa gaaatggtgc gttttgtttt
    49741 ttttaacgat tggattcgga ctgctttttg ttggtggatt ggtgtatgcg gcagaaaagc
    49801 aggtggatca atcgatctat gagattggga aatttcatat tgataccttt tcttcttatt
    49861 tatctgatag tgaattttca ttaggcaaaa tgttatttgg ggcaaatggc acagattttc
    49921 taaaaatgat gacgaatatg ttctttagtt taagtaaatt gatttggcaa ggccttgatt
    49981 ttgtcattga gaagctctat gaaggagctg caatgaacga gttaattcac cagtttttca
    50041 ctttttcgga tactatttat tcaaaaatat acgatcctat tggtgcgggg attgtcgtct
    50101 tgtatgtgtt atatctattt actcttcgta tatttagagg gaaacattac gcaaaaatgg
    50161 tgtttattcg atttctcttc gttgtcattt tttcaatggt ttggtttggc tatggtagta
    50221 acgtacctag taaaggagaa gcttttacca aagggttaaa tgatttgtcc gtggaggtag
    50281 aaggctttat tttttctgct acaaatagtc tagatgaatt ggcgacaaaa gatgtcaatg
    50341 gtgttttagt gccaacgaca caaaaggaag ccattcaaaa agtgcgccaa ctttattata
    50401 aagcggctgt tgttgatccg tatatattgt taaattatgg gacaacaaat ttagaagaat
    50461 tgtatcaagc agagattgaa ccacaagaat ttctagccaa ggaagcgtca agtgaagaat
    50521 tatctgatat tgagacaaaa gtgaccactg ctgcaggaga agatagaggg aaaaagaagg
    50581 cttatcgtgt ctatattcaa ccaactaaag ctgtttataa agcagtcgtt ggttctatgg
    50641 tgccattgct taatctaagt attggattac cacttgtagt gattggcatg attcgtttta
    50701 tctttcaaat gttggtgttg tgtttattac ttgtgattcc tttttcatta ctcgcttctt
    50761 ttgttccagt atttgattat atgctcatga ataccttaaa gtcctttttg ggcttgatgt
    50821 ttcaaaaatc tttttacagt gtgattattt tggtggcatt tttgatctat aacatagtag
    50881 acgatttaat tccaatgaat actgtttttg gttttttagg aaatatgttt gtcaaagcac
    50941 tcctatcaat tttactcatt gtaaaacgga aagatttatt aaagaaacta gggttaggag
    51001 ctgcagatca aactataaac aacacgaaaa acatagcaag tcattctttg tctcaaatga
    51061 aacaactgcc tggtcaacta tcaactgctg gacaaaattt ggcaatcaaa ggtgcaggat
    51121 tagcagggaa tgtgtatcga gcaacgaatc cactcgcttc tgcagcgata catggatttc
    51181 aaaagtttca acaaaaacgt acaccacaag aatcggtaaa agggaggcaa gtggttaatc
    51241 cctttgaact aggtgggcgt tcaaatcaag ccttgcctta tagtggtcgt catgttcgtt
    51301 atcatggggt agagcctgaa atgtcttcta ccaaatcgaa aacagagtgg aattctggtc
    51361 tttcaaatgg tttgattcca aattcgtcca tgaattcagt aagaccaaaa caacaggtac
    51421 aatcattggc acaattgagt aagcaacaag ggaataatca aagaaaacca ttgttaaata
    51481 aaggtatcgc ttttgttggt caatcgattc cgttgccttc tggaaaagta cccgtgccac
    51541 aattaacagc tgctggttct ctttcacaac agattcgcca agtgaaaaat caggcgaaaa
    51601 atgggagtta tccagcaatg cagcagttat tgaaccaata ctctgttcct aattcgtcgg
    51661 tagccactct atcaggaaat cgttctgtgc aacaaccggg gcaacgttcg tctgaaagta
    51721 aaccgtcgat ttccgcacca aaatcgttac aacctgcatt ttcttcttta gataagccaa
    51781 cagagcaatc ggaaaaaata gaaaaggatc ctaaaacact gccgattttg gacgcaaaac
    51841 caggaactga ttttacttta ccaaaaggac gaacagtcca aaaaccatta tcgaagaaag
    51901 gatgaggaag aaatgaagaa agggtgtcta ggttgtcttc ttttctttat tcttcccttt
    51961 tttctattgc cattcttttt ttcaggagaa ctttctgagc atggaggaga agctacaggt
    52021 ctagctgtcg cttttaatgg ggaatatacc gaaggattac cgattttttc agagattaaa
    52081 ggacgtggtc caatcacaga tgaacatgcg caagcagcag ttggtgcagc tgtaaagtat
    52141 cagttattac cgagtgtgat tctttctcaa ttaggctatg aatcagctta tggacagtca
    52201 caatctggaa gaaacgatac gaactttttt gggattacct ggtttcctgg ttgtccctac
    52261 ccacaaggtt ctgcacgtgg aattggtggt tctgaaggtg gtttctatat gaagtttccg
    52321 aacttaaaag cttgttatga ctattatggc tttatggtgg cgagccaaag caactttaat
    52381 caatgtgtgg gcaataaaga tcctggaagt tgtttattga ttctaggtcg tggaggctat
    52441 gcggcagcag gtattaccga agggagtccg tattatcgag gagcaatgag tatcataacg
    52501 tcctataact taacggaata tgatactttt gcgatcaagc attggaatag cctaccaaca
    52561 aaaaaaactt cgcttattgc aggaaaaagt aaaggcaatg cgaaggtttt ggatcggatc
    52621 attggacgat ccgtaaataa tggggaatgt tatggtggta cggcatggta tgtatctcaa
    52681 ttaggtggcc cacagttaat gggttctgga cacatgtttg cgatgttaat aggggaagat
    52741 tataactgga caacctatgg ttggcaagta atcatgaacc caaaaccaag tgatttacaa
    52801 gcaggagatg tggtgaattg gcaagcaggt ggtgcattgt ctcctggaat ctatggtcac
    52861 accggaatta taacagatgt ttcatatggc ggacaacatt tctctagtgt ggaacagaat
    52921 gccgagcaag gacggattat ggctcggtat caacgcacgt acaatcaaac aaaaattcgt
    52981 agtctggttc gtaaggtgaa ataagaaagg cagtgactag aaacgtgaaa aaagggataa
    53041 ttttagtatt tagttggtta tgtttgatag gtcttactgg ttgtcattgg ttttcttctg
    53101 aaaaacaaaa aacacaattg agaacgacag aagaaacaat cgaagcagta aaagaaacaa
    53161 gtggaactct tcaaagtact gaaagtaaca gtcaaattgg ttcattagaa gacgagacag
    53221 cgcaacaaat gcaaatagat gaaatgacaa aaaatttctt aaaagatttt gctagaaaat
    53281 ggagtaattt cacagatgtt tataaacgca atcaagctgt gagggactat atgacagatc
    53341 gttgtattaa agacaatagt attgatgtag acccgcatgt acaaatgaaa acaactggga
    53401 aactttacca ggtgacacaa gatatagaga atccggatca atatttgtta tttggagaag
    53461 aaacaatgaa taaggttact caatttgtat tacttcaatt gaaagtaggt caagaagctc
    53521 aaaagattga tgcgatcaag atttattatg tacggagtgc gtattaaaat aaaaaatgaa
    53581 cactcatatt tattatacac cgaagaaata gaaaaaaaca gactgttttt ttaagaaaaa
    53641 ctatgtcatt ctttacttga aaaaagttgt tataggacag aaaggataaa acaaaaacgg
    53701 aaaaaatatg tcttatgaat aactataaga actattctat tttttaggag atggatacga
    53761 gatgaaaaaa ctaattggct atagtgtgat gtgtattgga tttgttggtg tgggattgtt
    53821 tgtatcgact gaatctgctt ctgcggctga atggtataat cctatctggt ataatccgaa
    53881 cattgaatgt caaaaaggct atccgtatgg accgacaaat ctgaaaaatg gaaaatgtag
    53941 agtaaattgg aatggcgttg tagatagtgt aattactggt tcaatggact ctgctctaca
    54001 tggctttacg ggtcactaga aggaggaaga aaatgtctga agagaaaaaa gaaatcgttg
    54061 aatcattagt agctttaaaa gattctcttt ctaagattga atatgttgat aggcaagaaa
    54121 ttcaaaaact aattgatgat actattattg aaatccaaga tgcgcgttgt gaaggtataa
    54181 aaatttctgt agctttatct aaagtaatag aaaaaatgaa ccgttcatta gcgtttaatg
    54241 gattgaagct agatagacaa actagtctga tatgggatca tttaaaagac ttgtatgaca
    54301 agtctaaaag aagtgaacgg actgctgtat caatcttaaa aggcctttgg ggaatgaatt
    54361 cataaacaag atatttaaaa agtaacttat tttttatgga ggagaaatta tgaaaagaat
    54421 aattaaaata tttggtgttt taggtgtttt cttattaggt ttaagtactt ttgttagtac
    54481 tggaacaagt gtgtatgcca cagaaaaagt gaatacagtt gatttagaaa taaagaataa
    54541 attagtagat gaattaactt tttattttga tacagttggt tttttagacg aacatggtgt
    54601 ataccatata aaggattttg gtgctattcg tgaacgaatc agtcaagata acgaacaagg
    54661 aagagcagca agagtctttt atgaacatat tattgttcct agaatggaga aagttgattg
    54721 gaaacgatat gctggatgtg tagccattaa ttctcttcct ggaggaggag tggcttgggc
    54781 tataggtacg cagggagatt ttattgaagc attaaaagct tttaattttg aaaaagctgc
    54841 ggatttatta ttaaaaattg cagaaaaaca attaggaaaa gctgcagcta aagagttagc
    54901 taaggtaaat attgtagcag gaattgcagc agctgctgct agctgtgcat tagagtaaaa
    54961 gtttatgaaa agtttagaac aagagcatat ggcttttaaa caagcaatgt ttaaagaaaa
    55021 tatctattta aaccataact atattcgtgt ttctaaggca tgttcaccag tattgaatat
    55081 gcttggaggt ggaaatggat tatatcattt actgtttgtc gatgtttgtt ggttaatttt
    55141 tttaccagat gaactagtta tagtcaatga aaaaataaca agtaaaaatg aagtgtttaa
    55201 ttattcttta acaagaatta attacaaaga gattaccaag ttttcactag aaaaagttcc
    55261 tttttgggga gaattttgtt taaaaattaa atgtaactgg aaaagaatgt atttctatat
    55321 tgatggtgat gatgctctta cattcggtaa aactactttt tctagtttta attttcagtt
    55381 tctattaaaa aataattttt atggtttatt gaaataaggt tgcgaaggaa gatgtagagt
    55441 tatttgcact gttgtgttgg tatatctctt aatgctgtgt ctttttgggg agattaatta
    55501 gtggagtgat tatatgagta tagatgattg tttagaagaa caaataagat tatttttaaa
    55561 gtttttaaaa gttaaagttc aaaataacta cttcacatgt tatagttatg cacaccctgt
    55621 tttaaatagt cttcctttta attctttagt gcaaggattg attacaaata ttgttttatt
    55681 agtctgtaca ccaaatgaaa tatttgtaaa aaagataggc agtggtattt cttcagtaag
    55741 attaaaagag aaagaatata ctcaaaagat tattcatatt cctaagcctg aaattaagcg
    55801 ttttgagata aaagactatt ctgctttagg gctgttctca attggttact ggtgataaaa
    55861 aataagaata ctaaaactat acgtagaata taagtaatcg gcatcaatcc taattatgtg
    55921 attgaatgcc gatttttgtt acgcgctatt tttaattatc tgtaagaaga ataattttat
    55981 tttactcata ttcgttttca ctttgaagaa gtaaattgca atttcttata agttaagcag
    56041 ctatgcaaaa ggctatttat tctattgtga aatataagat gtagttgaaa caggatatat
    56101 agaatcaaaa tacgaatctt attttgattt ttctagtttt ttgaaaaaag caccctttga
    56161 tcgttgtatt agtggaaaag agaaaaagga gcatacttat catgtcacaa aactacaact
    56221 aaatatctaa acaacaccaa tttgatgcct attgaaaaaa agtattgcga aatgaagcca
    56281 agtctattag aaaatgaaat aatagaataa aagaaaatgg gaaaccgctg aatgatgtaa
    56341 atgaaggtaa gtatccacga actaattttg atgaacaaga tatctatttt ttatttgtat
    56401 tggagatact agtttaatat caaaagttat cagcagtcat aattagttat ccaatgtaaa
    56461 aagtacaaac aatagttgtt atgcagaata gtttatgaaa tatattacat aataagaaaa
    56521 caagcga
//
LOCUS       DAZBTF010000010        56059 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000010 DAZBTF010000000
VERSION     DAZBTF010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.010"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(160..705)
                     /locus_tag="ACHGNS_000670"
     CDS             complement(160..705)
                     /locus_tag="ACHGNS_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFN6936645.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(717..1409)
                     /locus_tag="ACHGNS_000671"
     CDS             complement(717..1409)
                     /locus_tag="ACHGNS_000671"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFN6936646.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1428..1712)
                     /gene="macP"
                     /locus_tag="ACHGNS_000672"
     CDS             complement(1428..1712)
                     /gene="macP"
                     /locus_tag="ACHGNS_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFN6936647.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1725..2288)
                     /locus_tag="ACHGNS_000673"
     CDS             complement(1725..2288)
                     /locus_tag="ACHGNS_000673"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFN6936648.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2516..3178
                     /locus_tag="ACHGNS_000674"
     CDS             2516..3178
                     /locus_tag="ACHGNS_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFN6936649.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3302..4498
                     /locus_tag="ACHGNS_000675"
     CDS             3302..4498
                     /locus_tag="ACHGNS_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFN6936650.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4599..5975)
                     /locus_tag="ACHGNS_000676"
     CDS             complement(4599..5975)
                     /locus_tag="ACHGNS_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN6936651.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(6012..6879)
                     /locus_tag="ACHGNS_000677"
                     /pseudo
     CDS             complement(6012..6879)
                     /locus_tag="ACHGNS_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
     gene            complement(6968..7993)
                     /locus_tag="ACHGNS_000678"
     CDS             complement(6968..7993)
                     /locus_tag="ACHGNS_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN6936652.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(8011..8685)
                     /locus_tag="ACHGNS_000679"
     CDS             complement(8011..8685)
                     /locus_tag="ACHGNS_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN6936653.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8869..9762)
                     /locus_tag="ACHGNS_000680"
     CDS             complement(8869..9762)
                     /locus_tag="ACHGNS_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFN6936654.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9836..11533)
                     /locus_tag="ACHGNS_000681"
     CDS             complement(9836..11533)
                     /locus_tag="ACHGNS_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFN6936655.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11526..12839)
                     /locus_tag="ACHGNS_000682"
     CDS             complement(11526..12839)
                     /locus_tag="ACHGNS_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFN6936656.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12839..16780)
                     /locus_tag="ACHGNS_000683"
     CDS             complement(12839..16780)
                     /locus_tag="ACHGNS_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFN6936657.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16889..17347)
                     /locus_tag="ACHGNS_000684"
     CDS             complement(16889..17347)
                     /locus_tag="ACHGNS_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFN6936658.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(17645..18163)
                     /locus_tag="ACHGNS_000685"
     CDS             complement(17645..18163)
                     /locus_tag="ACHGNS_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFN6936659.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(18165..18782)
                     /locus_tag="ACHGNS_000686"
     CDS             complement(18165..18782)
                     /locus_tag="ACHGNS_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFN6936660.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(18779..19633)
                     /locus_tag="ACHGNS_000687"
     CDS             complement(18779..19633)
                     /locus_tag="ACHGNS_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6936661.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(19630..20541)
                     /locus_tag="ACHGNS_000688"
     CDS             complement(19630..20541)
                     /locus_tag="ACHGNS_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6936662.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20582..21916)
                     /locus_tag="ACHGNS_000689"
     CDS             complement(20582..21916)
                     /locus_tag="ACHGNS_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN6936663.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            22124..22954
                     /locus_tag="ACHGNS_000690"
     CDS             22124..22954
                     /locus_tag="ACHGNS_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFN6936664.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVDGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(23198..23447)
                     /locus_tag="ACHGNS_000691"
                     /pseudo
     CDS             complement(23198..23447)
                     /locus_tag="ACHGNS_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(23638..23832)
                     /locus_tag="ACHGNS_000692"
     CDS             complement(23638..23832)
                     /locus_tag="ACHGNS_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936665.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(24337..24522)
                     /locus_tag="ACHGNS_000693"
     CDS             complement(24337..24522)
                     /locus_tag="ACHGNS_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936666.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(25372..25713)
                     /locus_tag="ACHGNS_000694"
     CDS             complement(25372..25713)
                     /locus_tag="ACHGNS_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFN6936667.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(25713..25943)
                     /locus_tag="ACHGNS_000695"
     CDS             complement(25713..25943)
                     /locus_tag="ACHGNS_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFN6936668.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            26230..26568
                     /locus_tag="ACHGNS_000696"
     CDS             26230..26568
                     /locus_tag="ACHGNS_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936669.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(26773..27335)
                     /locus_tag="ACHGNS_000697"
                     /pseudo
     CDS             complement(26773..27335)
                     /locus_tag="ACHGNS_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(28067..28648)
                     /locus_tag="ACHGNS_000698"
     CDS             complement(28067..28648)
                     /locus_tag="ACHGNS_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN6936670.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(28831..29457)
                     /locus_tag="ACHGNS_000699"
     CDS             complement(28831..29457)
                     /locus_tag="ACHGNS_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFN6936671.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(29479..30810)
                     /locus_tag="ACHGNS_000700"
     CDS             complement(29479..30810)
                     /locus_tag="ACHGNS_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFN6936672.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(30965..31366)
                     /locus_tag="ACHGNS_000701"
     CDS             complement(30965..31366)
                     /locus_tag="ACHGNS_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFN6936673.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(31452..31658)
                     /locus_tag="ACHGNS_000702"
     CDS             complement(31452..31658)
                     /locus_tag="ACHGNS_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936674.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(31753..33255)
                     /locus_tag="ACHGNS_000703"
     CDS             complement(31753..33255)
                     /locus_tag="ACHGNS_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFN6936675.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(33273..33728)
                     /gene="argR"
                     /locus_tag="ACHGNS_000704"
     CDS             complement(33273..33728)
                     /gene="argR"
                     /locus_tag="ACHGNS_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFN6936676.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(33826..34443)
                     /locus_tag="ACHGNS_000705"
     CDS             complement(33826..34443)
                     /locus_tag="ACHGNS_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFN6936677.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            34664..36037
                     /locus_tag="ACHGNS_000706"
     CDS             34664..36037
                     /locus_tag="ACHGNS_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN6936678.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            36112..37224
                     /locus_tag="ACHGNS_000707"
     CDS             36112..37224
                     /locus_tag="ACHGNS_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFN6936679.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(37296..38933)
                     /locus_tag="ACHGNS_000708"
     CDS             complement(37296..38933)
                     /locus_tag="ACHGNS_000708"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFN6936680.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(39101..39709)
                     /locus_tag="ACHGNS_000709"
     CDS             complement(39101..39709)
                     /locus_tag="ACHGNS_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFN6936681.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(40093..41244)
                     /gene="queG"
                     /locus_tag="ACHGNS_000710"
     CDS             complement(40093..41244)
                     /gene="queG"
                     /locus_tag="ACHGNS_000710"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFN6936682.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(41263..42015)
                     /locus_tag="ACHGNS_000711"
     CDS             complement(41263..42015)
                     /locus_tag="ACHGNS_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936683.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(42156..43105)
                     /locus_tag="ACHGNS_000712"
                     /pseudo
     CDS             complement(42156..43105)
                     /locus_tag="ACHGNS_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
     gene            complement(43106..43945)
                     /locus_tag="ACHGNS_000713"
     CDS             complement(43106..43945)
                     /locus_tag="ACHGNS_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFN6936684.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(43942..44688)
                     /locus_tag="ACHGNS_000714"
     CDS             complement(43942..44688)
                     /locus_tag="ACHGNS_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFN6936685.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            44926..46296
                     /locus_tag="ACHGNS_000715"
     CDS             44926..46296
                     /locus_tag="ACHGNS_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN6936686.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(46351..47247)
                     /locus_tag="ACHGNS_000716"
     CDS             complement(46351..47247)
                     /locus_tag="ACHGNS_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFN6936687.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(47361..47531)
                     /locus_tag="ACHGNS_000717"
     CDS             complement(47361..47531)
                     /locus_tag="ACHGNS_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936688.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(47528..48205)
                     /locus_tag="ACHGNS_000718"
     CDS             complement(47528..48205)
                     /locus_tag="ACHGNS_000718"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFN6936689.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(48296..49201)
                     /locus_tag="ACHGNS_000719"
     CDS             complement(48296..49201)
                     /locus_tag="ACHGNS_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFN6936690.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            49376..50800
                     /locus_tag="ACHGNS_000720"
     CDS             49376..50800
                     /locus_tag="ACHGNS_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFN6936691.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            50846..51877
                     /locus_tag="ACHGNS_000721"
     CDS             50846..51877
                     /locus_tag="ACHGNS_000721"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFN6936692.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            51884..53071
                     /locus_tag="ACHGNS_000722"
     CDS             51884..53071
                     /locus_tag="ACHGNS_000722"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFN6936693.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            53227..54609
                     /locus_tag="ACHGNS_000723"
     CDS             53227..54609
                     /locus_tag="ACHGNS_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFN6936694.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            54646..54966
                     /locus_tag="ACHGNS_000724"
     CDS             54646..54966
                     /locus_tag="ACHGNS_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936695.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(55166..55834)
                     /locus_tag="ACHGNS_000725"
     CDS             complement(55166..55834)
                     /locus_tag="ACHGNS_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFN6936696.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT        16145 a        11703 c         9928 g        18283 t
ORIGIN      
        1 aatataactt aagttgttga taataaagag atttattaac gtaaaaagaa taagtttttt
       61 taaaataacg tataaaaaag agcataaaac aaagtcgttt gttttatgct cttcttttat
      121 tattttgtgc tttttatttt atcaaatcac tttgtttttt tataaaattt ctgcgcctag
      181 tgtggactca aaatgtctca atgcccattc atgtccaacg ggatcgaaac ttgccacgga
      241 atctttgaaa atatggagtt tgtatcctag attatacgca tcgactgctg tatgcaacac
      301 acagatatct gtacatacgc cggtcaaata aatatctgtg atctggcgtt ctcgcaaacg
      361 gatgtctaga tctgttccac taaaagcgga ataatgccgt ttgtctatcc aataaacatt
      421 ttcctcttgc tcatagtctt ggtataaact ttttaaagag ccgtacaatt cccgtccacc
      481 cgttcctatc acattatgcg gaggaaataa gcggttctcc ggatgaaagg aatcttttgg
      541 atcatgcgca tcaatggcaa aaacgacaaa atccttttga tcaataaact tctttgtatg
      601 agcagtcagc tttttttcaa ttacttgtcc tgctgtccca gtagtcagct ttccatcctc
      661 tgcaacaaaa tcatatgtgt agtcaatcga gatcaatgcg ttcatcttag tcctccttat
      721 acgagatgtt tgacaaattc gatcaccatt tctgctgatc gtttgcctgc gtcttcgata
      781 aattcgtcaa agctttgtgt cgccgagtga tctgctgtgt cgctcattgc acgtacgatc
      841 aaaaatggga tattgaactg acgagctgtt tgtccaacag cggctccttc catttcgcaa
      901 gctaatgctt cagggaaatt cgttaagatt tctttgattt tatctggcga gtcgacaaat
      961 gtatcacctg taacgatcaa tccttctttt gcgttcaaat tcgttgcttt tgctgcttta
     1021 accatttcac tgcgcaaata tgtgcttgct tcataatata gaggcattcc tggtaattgt
     1081 cctgggttgt agccaaatcc tgttgcatct gcatcaaaat aagctacttt gtctgatata
     1141 acgatgtcac ctacttgtaa gccttcaccg attccgccag ctgaacctgt attgatcacc
     1201 atgttcacgc cgtattgttg gatcaacaga cttgtggtaa tagatgctaa gacttttcct
     1261 ataccggaac gtacgacgat cacttcgtgg ttacctaatg aacctgaaat aaataaagcg
     1321 cctgcgcgtt cccatgataa tggttcgctt aggttctctc tcaaaatttt tacttcttct
     1381 tccattgcgc cgataatacc gattttcatc tttttaattc cttctttcta tataaatgcg
     1441 attgctaaaa aaaccacaca taacattagg ataacaataa taagagattt catcaaaaaa
     1501 gagttccatt tggtcgtctt ttcccgttcg ctgatccttg ttttcgtgat tggtttattc
     1561 tttttgcttt ctttacgata aaagctggca atcttttttt cttcttgctg ataagctgtt
     1621 tctgcttttc gctgtttttt taatgactcg cttgcttgtg cttgttgtcg cttgcgaagt
     1681 tcgcttcgcg taataagcgg tcctttggcc ataagccgat ccctctactt tcctttggtc
     1741 aatagttccc agtattgaat cgcataaatc gtctttgcat cacaaatcaa ctgatctttc
     1801 atcgcttgtt gcgcctcttc aagagttaga tgatacaact ccagtacttc gtcttcatct
     1861 tgtgctaaag gattctcgac cttctctacc ccttgtgcat gataaatatg aaggacttca
     1921 tttgcaaaac ctggtgaaag atacatcgaa gtaagatggc tcagcgactt cgcacgatag
     1981 ccagtttctt cttctaattc tctagctgct gtcatttcgg gattttgtcc ttctcctgga
     2041 tcgatttttc cagctggaat ctccaaaatc actttttcta aaggttttcg aaattgtttg
     2101 acaagaagca gacgatcttg ttcgtcaaaa gcaatgatcc cgacaccgcc aggatgaaat
     2161 accagttctc tcttcgaagt tcctccatct ggcaaacgaa cttcgtctac agcaacatca
     2221 ataatttttc cgctatagat ttctttgcga ctgattgttt tttcttcaaa ttgctcattg
     2281 ttcagcatat cttctcctgc tttctttgta atcataactg cttttttttc atttttcaat
     2341 ttttctcaaa aactccttat tagtttacca tagataaacc ttagattttc tctaaatata
     2401 gaaaatcctt ctttagtccg atactctttc tagtagtccg tatgctaaaa taagtatgta
     2461 atcgaataac gaaaagaatt caatactcaa cgataaatga catggagggt ttattatgat
     2521 aaaacgaaat tggccgtggc ttctagcatt ggtcttgcta tttctctttg tccgaaacaa
     2581 tagtttttct tttgttctag gacttttatt gataggtgta gttttagttt tgtggggact
     2641 ctttggttcg cgcaggaaaa agggaaaaca agaagaaatt cctgcactga ctgacgaact
     2701 tgaaactcac tatgaaaaaa gcggtatgac agcgaatgaa atttcatttt ttcgacaaac
     2761 aatgaatcaa acaaaaaatg agatcaatca attgcaaatg aatatgcagc agacagccaa
     2821 acttaaagca atcgatctcc gccatgatcc agtcaaagca gcgaaagctt tattcaaaga
     2881 attggtgaaa gaaccaaacc gactgcatga agcaagtcag tttctttata cgcatctgcc
     2941 gaatctagtt gatttgacaa ataagtataa tgagatcaat gaccatgaga tcaaaaacaa
     3001 acagacctat gaaaaactag aagaaagcgc gcagatcatc gaccagctgt cagctttaat
     3061 cgcacaagat tatcaaaaat tcgttgctga ggatctagat gatatcgacg tagagatatc
     3121 tgttgcgaaa cagagcttaa agcgtgataa taaatatgaa ccagaacaaa aggattaact
     3181 cttttgttca taaaagaaga ctcggaacat tcagatgatg tctggctgtt tcgagtactt
     3241 tttctaaagt agagacattc atgtaagaat cgagaacaga ttcaacaagg aggaattttt
     3301 tatggatcat aaaccgactg aaacaaaaga tgttacacca gttaacgata cacttgaaga
     3361 tttattgaac aacccgtttt caactccaac agatacgctg accacttcac aacaagcgga
     3421 gattgatcag ctccaagagc aacagacagc tgctcgttta attgacaaac taccggcgga
     3481 acgccaagaa caagctaaac agctagcagc aaaaatcgat gctaatgatg cacagtctgt
     3541 catcagctac ggatcggctg cccaagcaaa attaagtgaa ttctcacaat cgatgttgaa
     3601 tcatgtgcaa gcacaagata ttggtcctgt aggagattcc ttaactgaac tgatgtatcg
     3661 tctccaagaa gccaatcctg acgaactgcg tgccggagaa gggaacatct tccagcgtgt
     3721 cttcgggaag gtcaaacaat cgatctatga agtgacagca aaatatcaaa agattggtgc
     3781 acaaattgac aagatttcag tgaaattaga taaagaaaaa gacggtctgc taaaagataa
     3841 tctgatgtta gaacagcttt accagaaaaa caaagactac tttgatgcac tgaatattta
     3901 tatcgctgct ggagaattga aaatggaaga acttcaaaca acgatcattc cagaagcgat
     3961 gaaaagagca gaagaaactg gcgatcagat ggatgttcag attgccaatg attacacgca
     4021 attcctagat cgtctggata aacgtacgca tgatcttcgc ttagctagac agatcacgat
     4081 ccagcaagca ccacaaatcc gtttgatcca aaacacaaat caggccttgg ctgaaaagat
     4141 ccaagcatct atcgctacag ccattccgct gtggaaaaac caagtcgtga tcgcattgac
     4201 cttgcttcgt caaaaagatg cagtaactgc tcaacgccaa gtctctgaaa caacaaacga
     4261 cttattgaag aaaaattcag aaatgctgaa aatctcagcc atcgaaacag cgaaagaaaa
     4321 tgaacgtgga atcgtggata tcgagaccct tcaaaccact caaaatgatt tgatcgaaac
     4381 aatccaagag acattgcgta tccaaaaaga aggaaaagaa aaacgtcgtc atgcagaagt
     4441 agagcttgga cacatggaag aagatctgaa acaaaaactg ttggatttaa cccaatagat
     4501 cacctttcgc ttgttactaa aacaaacgcc caacaacagt ttattacaac tgttgatggg
     4561 cgtttttctt atctttcttc taacttctcc attggagtct atttctcatt catcttgctg
     4621 tgtccatagc catagccaaa ataaatgatc agtccaataa ccattacgat accgaacacg
     4681 atccatgtca cagtttgcag acgaagcatc aatacaatac aagtcaaaat ggagacgatt
     4741 ggcaatagcg gaacgaatgg cactttgaat tctccagctt ttggtttgcc aaacattttt
     4801 ctcaacttca gtaagcctag actaacaagc atcaaatagg cgagcgccat gatattcgtt
     4861 agttccgcta atacattcaa tgggaaaaat ccggcaaaga atacagctgc cacaccagcg
     4921 gcataagtcg cttttttcgg tgtacgatgc ttttgatcga tttctgtcat aaacttagga
     4981 agtaagccat ccttgctgac agcaaacagt aaacgagcaa gtgaatacat catcgagatc
     5041 gttaccgtca gtagcgttaa aattgcccct acagcgatca ccgcgcccac tgttccatgt
     5101 cctacatacc tcattgcaaa agccactggg tcattgactc ctagttcagt atacggaacg
     5161 attcctgtca agatcagcgt cacgatgatg taaaggacag tagcaatcag tatcgaacca
     5221 atgattcctc ttggcacatc tttttgcggg tttttgactt cctcggctgc cattgcaacg
     5281 gcatcaaatc ccaagaaagc aaaaaacaca agagctgcac catctagcac accggaaaaa
     5341 ccaaaaggca tgaatggctg ccagttatca ggtttaacga aaaatattcc tacaatgata
     5401 aacaacgcga tgataccaaa tttgacatat accatggcat tattcaaacg taatgctttc
     5461 ttggcttcaa gcgacacgat gtaagtgacg aatatcacta cgagcaccgc aatcaagtca
     5521 atgtatgtac cattttccgg attgtacccg ccagttaatg cttgtggaag ttcggcccct
     5581 aaacttttca aaaaaccttg catatagcca gaccatccgg aagcaacaga ggaaaccgct
     5641 agtaaaaatt ccccgatcaa caaccaccct gtcagccagg cagcaaattc accaaataca
     5701 acatacagat aggcatatgg cccaccaatc actggcactc gcgaagcaaa ttctgcgaaa
     5761 cttagtccag acaaaatgac aacgattgca gctaagacaa atgaaagtga cagtgctgga
     5821 ccagcatttt gattggccgc aacccctgtc accacaaata ttcccgtacc gatgatcgca
     5881 ccaattccaa gcatgatcaa atcagacgtt tttagttctt ttttcatctc actcgtatga
     5941 ttgagattga tctctttctt acgaaacaaa tccattgaag ttcctcccca ttttaggttg
     6001 gaaaacagcg attaaactgt tttcatatac cctaaaaggg ataccaccta aaatgactaa
     6061 aaggcaacca acaataacat aaatgagctc ttttttcgtt tttctttcgc caaggatgaa
     6121 aatcccacct aaagtagaga taatgatccc catttgtgaa agtgagaagc caacagctaa
     6181 accaagtgat ttgactgtta gaagcatgca gatgtttcct aagccccata ataatccaca
     6241 gatcatgttt ttccacacaa actgatcgac tttaacttta cggaaagcaa agaagcttgc
     6301 gcctaagatc attccgatac tttgaggtaa gataattgac atcgcatcca aattcgccca
     6361 gttgacgatg atggtatata caccataacc aatcgtagag aagatcaatg ctctaaaacc
     6421 cttaccaaaa tcaagcattt tattatctgt atctattttt ccttcgtcat cttgtcgggc
     6481 agtcaaatac gcaccgctta caagaagagc taaagcaata ataccgtata cataatcttt
     6541 cgtttgtgtc cattcatgga agaaaacggc tcccgcaatc gtattcaaaa ttaattgaaa
     6601 accagtagac aatggtagcc ctacagaaac acccatgtac ttcattccat ggaactgtcc
     6661 attttgtcca acactccaag ctaagccgga taaaaatccg atcaagaaaa tccaaggggt
     6721 gattacagga ctaacaacga aaaagacaac taaagaaaac agaaatgcac cgatcgtcat
     6781 tcctagtgtt tgttgattgg catcgccacc aattttacca cttactaaac caatactacc
     6841 ccatgccaac atagggacta atgctattaa aatttccata accaatctcc ttttatttca
     6901 catgataaga aacttatcag cgaaaacgtt aacaacacaa gcactttttt tcgtttctaa
     6961 cgattttcta acgtttttca ttataaaaat ttaagattgg gaaaaataaa gtcattgttg
     7021 tcccttttcc ttcagttgat tcagccgtca aatggatacc taatttttcg gccagatttt
     7081 ttgctaaata aagtcctaat cctgttgaat gtgttttgct aagtcgacca tttctcccag
     7141 taaatccctt atcaaagatc cgtttcaagt cttccgctgg gataccgatc cctgaatctt
     7201 taactgaaag gctgactcct tgcgcttcct tttctatgct cacaatgatg tttccgtgat
     7261 ccggcgtata tttgatggca ttactaagca attggttgaa gataaaagcg atccatttag
     7321 catcggtcaa aacagtatga tcctcaccaa cgatctcgta gcgaatattt ttttggataa
     7381 aataatttgc ctgactgcgt atcacaggct gaatgattcc ttttagtcca tattcttgga
     7441 tgaggtagtc ccgcgagaaa ctgtcgagtc ttgcataata caaaacttgt tctacgtatt
     7501 catcgatttt tgataattca ttttctagta atgtatactt ttgatcgtca atatcgaact
     7561 cgatcgaacg aactagcaat ttagaagcgg aaagtggcac tttgatttca tggacccatg
     7621 agtcaatata atctttttgg tcttgttgat tactgattgc tgattgcatt acttcttggt
     7681 gttcacggat cagctgattg atatattctt ctaccagttt ttcttcttct gtcctcccac
     7741 cactcaaata attttgcaaa ggggaagttt tttgtatgtc ggctaatttt ccccaccatc
     7801 ttctcttgct caagtaacca cccagcagat aaaaaatcag aaagacgccc tcaatcaaag
     7861 ctaaataagc aattgtgctc aaatcaactt tgatgtttgg cgccagccac atcacaaaga
     7921 tcgtcaatgc aataaaaaac agccatccca agatcaatgt ccactgatct ttcaaatatt
     7981 taaaaaaatt catcttaacc gctcatcctt tcagggaacg atatatccct gtcctacttt
     8041 tgtttcgata tatccttgga tacctgcctg ttcgattttt cggcgtaacc gattgatgtt
     8101 gactgtcaat gtattgtcat ctacaaatcg ttcgtcatcc cacagtgctc gcagcaattt
     8161 ttcccgactt aagatctttc cattgtgctt catcaaaata aataatagac gatattcatt
     8221 tttgctcaaa tcaatgatct ctccgttgat ttccatactt ccgttgtcaa cattcaacgt
     8281 aatgccattg tggctcatca tttcactaga taattctgtg tagttgtaag aacggcgaag
     8341 taatgcattg atttttgcaa tcagtacatc gatttggaaa tgttttgtca caaaatcatc
     8401 tgcccccatg ttcatagcca tgatcatatc catattcgta ttcctgctgg agataaaaat
     8461 gatcggtact ttcgatactt cacgtatttt ttgacaccaa taatagccat ccaaaacggg
     8521 aagattaata tctagtaata caagctgtgg ttcttctctt tcaaagtctg agaacacttg
     8581 attgaaatcg gtcgttccaa aggtttcaaa ttgccatttc tcaagttcct ccatgatcaa
     8641 ttgtcgaatg atctcttcgt cttcgactac catgattttt gccataaaaa atccctccat
     8701 ctttcttctt gctactacaa tatcaaagaa gcccttgtga aacaagttgg cttacctgga
     8761 aaatgtcatt ttagcaaacg ttaaaaaaaa ctggaaatat gcgatttaat cgcgtttgtc
     8821 tcttccaagc agcttaaatt tattatgtta ttcttaataa ttggtctatt atctgttatt
     8881 tttctttcgt tgtttttgtc ttttaaattt tcgataacac caataaccga aaagcgttag
     8941 cactagcaat atacctagaa ggagtataag taatcgataa agatggtatt tttttgtctg
     9001 ttctatcttt gaagagactt cttctgtaaa gggaactcgt cttcctgtca ctagtaatcg
     9061 gtgagtgttc acggtgtaag gtgtacaagt gataagtgtg actagatcct gcttttctct
     9121 aattgctaat gtatttgttt ttgttggaag gacaacaatt ttctcaacga cttcataagc
     9181 gagcatccga ccatacactt caagatagaa tcggtcatct tgctccattt cttcaagatc
     9241 cgtaaagagt tttttctcag caataccgct atgggcggta atcacggaat gtgtactctc
     9301 tcctccagtt ggaaaagagg tgccttgcag gactgtcgct cctctatcta acaaaagatc
     9361 attcgtttca tcaaataaag gcaagctaac attgattttc ggaatataga tggctccgat
     9421 catatgttgt tcgtaatatt cttttcctgg attttggaca tttctaaccg cttgctcaaa
     9481 aggatctttg acttgtccca ttcctgggat tactgatgta tgagcaagcg tctgattttt
     9541 cttttcatgc actgctaatt gttgctcatg tttttcttct ttatcgcgtt caagctgctt
     9601 ttgataattc tcgatccttc tttgatccaa ataattgctg agagcatctg ctaaaaatgg
     9661 atatgtaaaa actgcagctc cggcaagaaa catcactgcc ataaataatt gtaataaaag
     9721 actgatttgg gtttgaggtc tattacctat tttctttttc attttcttca ccttcttatt
     9781 taaaaaaact gcagcaacct ttataaaacc gattgctgca gcttattttt gttgcttata
     9841 cttcagactt cattttagat ttacggtacc agctgtacgc tccaatcatc aaacttattc
     9901 cgattagtaa gaatgccaag agcccgttcc caccagtagc aggaagtgac ccgcgtttca
     9961 tattcggtac tctttctggc cctggatttt ctactcctac gatctgaatt tcttcattat
    10021 agcttccata agagatgata aattcagtaa aggctgcctc acctgttgga agtacataac
    10081 cttctggcgc catggtctct ttcatttgat aggttccata atctaaccct tgaacctcaa
    10141 agtatccatt tccatctgcg acaaattctg ttgcttgtcc gcttgttgcc cactcaattc
    10201 tatctgctcc tgattcataa acagctaaag gcgactcccc tttataaaat attgcgtatt
    10261 cagaacggtc ttcattccac aattcgaatc ttgcatcttt aagcaattca ctgcttgatg
    10321 catcatattt ttcaaacttt cttccacctg ttgtgacttt ctctggcggc tctactcctt
    10381 cttcataatc aaattcatca tttcgtccga tttcaataac cgctgtgttg ttatggaaat
    10441 catccgcttc tagttctgtg gaaaccttca aattatacgt gatcgttaaa tctcctccac
    10501 gtgcatctgc taatctacct aaagtatcta ctttggctac tccattttct tctgtcaaaa
    10561 tcgtcacgac aaacccgttg cttactactt ctactatata atcttgtcct tttgttaaaa
    10621 tcgtctcgcc ggctcgcact tctatttcct gatcataata agttagacca actgtaggcg
    10681 tatctgtgat tttgtaattt ttgataacag cgcttccgtt ttcaaggaca gaatcgatga
    10741 aataaggaat aggtgctgtg atcgtgtaag aagccgtttt tcctctttct agatcaatat
    10801 aagaaatcgt ttcttgttct ccagcttcat taataatcgt tacttgtcta aggtcgcttt
    10861 cctctacatt catctgttta tctgcttctc tgatttcgtt tttcggatac aaatgcacat
    10921 ctgtattgta tgtgtctttc catgtagaac catcccacac gccatccacc atatcaggca
    10981 tcatcactgg catcataaca accattggag acgcgattct ggaaatatga gctggcgaat
    11041 aagtttctaa gaaaagatag actttatctc tagaagaagg tgcttcttcc gtcacttgga
    11101 cggtagagaa taccgcttct ccagcttcat tcgttgtttg tttgtcaata cttgtcagtc
    11161 gatattttaa aaaccaactt tctgtgtgcc gtttttgaat ccattcaatc gcttcggcag
    11221 aattaagacc gtcttctcgt tccggtgtta aaggattatc tcgaatcaaa tcgtaatata
    11281 cttccgttac gtcaaacaaa tcaaaatcga ctcctggaat attttcttca ccaaaatccg
    11341 tagaaaccaa tccgctattc ggtcgttctt ccggaacaga tgagaaacct tttttgtgta
    11401 atgtaatcgt cacttcactt ggtttttgtg cgacttcttc ggctttgact agttttccgt
    11461 tcccgaaaat ccctattccc gcagtaacga gtaacagtac actcattccc gtgacaatca
    11521 tttttttatt catttttttc tccccgttct ttttttatta ataaaacagc acctaacaat
    11581 aaaagaattc ctagtaaaga aatcaacgtt cttgcttcat tcgtttgcgg gaatacggac
    11641 cattttcctg attgacctgg attgcttgga ccactcggct ctttcggttt gtccgttgtt
    11701 tcttttacgg tctcgttata aacacgtggt gttgcggatt cataggcaga gactggaact
    11761 ttcccggatt ctaattcagc catctcctgt ccgttaacta ctacatattg tggttggcct
    11821 tttgcatcca cccaaaattc agggatgatg acctcgattc tcttacttgc ttcatccatc
    11881 tcgtaacctt ccacaccttt taattcttca aaataatagg tgccagaagg caagaaacgt
    11941 tgtcctgtcg taacaaggcc ctctttatcc gaaatgaatt tgcttacatt tttgtcattc
    12001 aaaggatcat tgtctgctgg tttgatccat tgatttttca agtcattagc aggagccata
    12061 tctaaataat atttcccccc ttctatcatc tgatatagag caaaaacagc accttctaat
    12121 ggttttcctt tttcagttgt tcctctttga cgtccatact tgaagaagta tggatctctt
    12181 agataaccga catttttagg atagatatga atctcttgca gttcctcggt tgattctgtc
    12241 gggtgataaa ttggcaaaat agcagcaatt gggatagctg tctgatcgat atcaacatcc
    12301 aatccaactt ctggatcaat cgattcttcg aaaatcaaat aaactgcatc ccgatcttgg
    12361 tttttcttag gtgctttgat acgtgcaatc ccgtctccta atccgccaac cgtatctttc
    12421 ccagttgtta ccgaagtccc tatttggaca agcctttctt ctgtgattag ctgacggata
    12481 ttgtttctgt tcgtttcagc gatttgctgg acgaattctt taggagtcat gttttcagca
    12541 gctttttttt catgataata atcggtcatg tcgtaaatgg caaatgtcgc tccatttagt
    12601 ggcaccgtct gtgaaatgat atctgctgtt tcccctgctt cctcagagat agccaagcca
    12661 tcattttcat ggtattcaaa ttgatcgtta taatcgatat ctcgaatcat ccgtttatgc
    12721 aacacgaatt ccacgacatc ttcccctgat tcttctgccg acatcgcatg tggaagaagg
    12781 actgctgcaa gcggtagtaa aacaagtaat tgaagcatca aacacaacag ctttttcatt
    12841 accttgctcc ctccttttta gattgtctga caaacaaacc ataggctgct cctgcaagcg
    12901 ccatgaagaa tgcagcaagc aggatataga ataatcgtcc ggatcctcct gtagcaggca
    12961 acggattttc aggatcgttc ccgacactaa gctgtaatct gattcgattt gtctccaaaa
    13021 gttcatattc taactcttgc tgtagatcag gcgtctctgc ttcttcgttt ggataatacg
    13081 aaatctcaac ttttagtgta cccgcttctt ttcctggaac tacccgatca ttttctatgt
    13141 caggttcttc tcgataacta atttctaaaa taaagtaacc tggaagtttt ctatagctgt
    13201 caggtgactt cacttccttg atccgataga ccccttccaa caagtctgaa gcattgaata
    13261 gggaagaatc gtttggatct ggtgccaagc ctccctcgat gatttcatac tctcctgcac
    13321 tattttgttt ctcaattgaa aattctgcgc cttcaagacg tcggctattc atactgtcaa
    13381 ttttcaaaag ttccaaatcg attggcgcta gttcatcttc taaggttact tgtagtacac
    13441 catcgatgat ttcatagcct tccggcaagg gagcagtttc gtcttcgatg accgtatctt
    13501 caatgataaa cttaccttct tcattcagtg caaactggaa cacttgatca gatagccgat
    13561 acccatttgg tgcgacagat tccgagacct catattcacc aatagacaga tgcagaggtt
    13621 ggtcgtaatt taaatcgaca tacttttcta ctcctacggc acgagaatag aagtatccga
    13681 tcccaggcaa acgtttcgat tcttctggtt ctaacggatt accagtgtat gctcgataca
    13741 gcgggaagga agaatccccc actttcctga tatcgaaacc tgcaccatct aatgtatggc
    13801 cttcatggtc taattttcta actcggatct cccgataatc aaacgtattg taagcagtga
    13861 atgctaccca taaacgatcc tcttcatctg ttgtatacgt gagatattgc caattttctt
    13921 cacctgtttg cttgagttgg atagtcccta tagcattatc gtaaagtcgc aagatccatg
    13981 tgtcattttc atcaggttct ttgaaacctt ctgggttctg gctctcaatg attttgtatt
    14041 ctgcatagtt ggtagtttct tcactatcct tataaacaaa tgaatctggc tctccgattg
    14101 gatgaataac tttctcacca tccacgatta catatttccc gtcttcatct actgaataga
    14161 acgtgagtct ccctttctct tcctctactt cgttcacacc agaaagccct ttcgctactg
    14221 gatatccgat ttccagtccg ttaccatctg tttcataaag ttcaaactct gcgccattga
    14281 ttggcatatt actgccttca attagtttat tgatattcat gtggattggc ttgaattcat
    14341 ttcgcatttc cagtttgaca tacaactcat catactctgt gccttctatg ttaggctctt
    14401 gtacaacaga aagcttgtga tattctcctt cataatagga attcggatct ttaccttccg
    14461 gtccgccgac tctcgaacca gatgtatatt catctacaag ctcgtagtga cctaacatct
    14521 catcttcgtt ggctgcatat tttttgattt ggaattgata cgtctcatct actaactgat
    14581 aacgtggtgg tccagcgatc tctctgatcc aaaatatgtc gctgactgct ccagtttcat
    14641 ctcctaaaat cggcatcttc gtttcaaaga tcgctattcc gtccgtggta gttttacttg
    14701 tttcgattgg ttcggcttcc ccactctctg gattttggtc atataatgca aattcgactc
    14761 cagccaacgg ctcgccattt tgacccttct tgctgacttg gaaattacgg cctttatgtt
    14821 cgtaacgaat ggaagaagat gggaaataca tccgtccagt agtgatatca ttctcataat
    14881 cctccaggtt ctgaatatag gccatattgt tcactgtgca aaaatttccg tcttgagctc
    14941 ccgcatcaag ttttacttgg tacttgatcc tgaccatttc cccttcacct aaagaaatcg
    15001 gagaaaattt ataagcttgg ttttcccgtc cgacatacgt tgtcggagtc gccactactt
    15061 ctggtggggc ttgtccatga cgttcttcat cccatatttc tggctcttgg tacttcatgc
    15121 cgttttctcc tggatctaga tggtaaaact gaatatctgc cgatctgcca gctccaaatg
    15181 gtgagacatt aaaaccagtt tcatcataaa gtgtggcttg ctggatcgtt ctcttgaagg
    15241 tagaagagat atcctccaag gctgtaacaa tgtcattctt gttgttcgca ttgtaatatc
    15301 gcggtgttac gccatcttct tctaaatcag caatattttt caatacattt cgtccaatag
    15361 catctagtcg ttgtctcccg ctaacgtttc catcgattcc taaaccaatg gagaagatac
    15421 ttggcgggtt ctcttgatac ttgtttttca gccaatgatg ataagcaact gtgttgatcg
    15481 cagatgactt ttcttgcgaa gtaggactgc cattcgtatt gttattagaa gaaccatcac
    15541 caacccagtg agtcgcttta ttatctcctt cttttacctc tccataacga tagtgtacat
    15601 ttacttcatc ttctggacgg ttaaagtcag atgcataggt aactggatga gtaaaccctg
    15661 cattagaagt attcccagaa aactcctcaa aatttctgaa caaaacgtca ttatcttcag
    15721 caatcttatc ttccatcaca gtccagttgc tccaagctcc attagtgctc gtacgataac
    15781 ttgactgaat cggtgcataa ctgaaggtcg gtgtcccatc tcccacaacg attaggattt
    15841 tttctggatt tctattttct ccgccattgt ctttatacaa tgtttcatag ccatttttaa
    15901 gccctagtgt aagcggagtt cctccgattg gtgtacgatt taatgctgta ttctcacttc
    15961 ccgtcaaacg attgatatca tttgtcaatg gaaagatgtc cgaactgatt tgctcatggt
    16021 tattgatagc tgtactatta tggtaaaaat tcaccatacc gattcgaata tccagactcg
    16081 ggtcagagag cagatcttta ctcatttcgt ttactgcctc aatcaacgca gcatttttag
    16141 tttgactatt ggtcccagcg gttaattcac tcatactcgc tgacttatct agaacaaaaa
    16201 cgatatcgat tggactgatt tcctttttct cgcccccgat gacatctaga tagacattgt
    16261 aaagggttgt tgcatcttca ggatgtagat cagatgctgt gctgcttggc agaacaggtt
    16321 ctacccattt tttgatgtac gccccgttat aatccaagta actgttttca aaattccgtt
    16381 catttcccca aagctgaacg gtttgagctg gaacgtttct atcttcttcg cctgcagttg
    16441 atccattatt agtagcatag tcgtaatttc gccagttcgc tcccccgttt gtataatcac
    16501 tatcatcgat caagttctct ccgtcatatg gcgagccttt gtctccttga tagcgttcat
    16561 tgctttgtgt ccaatttcct aacacttttg tatagtcatt cgttgtaaac ttcgggtagc
    16621 gatgctgttc gttttcaact tctccgctag gatagatata ttcatagggg tcttgcgtag
    16681 ctgttggccc aagacgctcc aaaataccgt tgaataactg gcgttgaatg cgctggagtg
    16741 cttgaccgct gggtacgatt ggttcgtcca gcttttccat ctcattttct tcgctacgtt
    16801 gaatttcagc ggaatcttct gtatcagttt cattttcttg taattcttgg ttatctattc
    16861 tttcatcaac caccacttga agaggtagtt acccttcgtt cacatcgggg atcttttctg
    16921 tttcaaaaat caatgtgccg gtttctgctt cttttgaata aacttttccg acataccatt
    16981 cttgaccatc gatcgtctcg acttttaggt cttctccatc ttcatcatag gaggatagac
    17041 ctgttccacg aacgtttttt acggtaccaa tcccggcggc tgccgcatcg attgctaaac
    17101 gtattctacc ttcattggca ctttcctttt tttcaaaagc cagatgccat tcaagggttt
    17161 cttctttttc aatatacttc ccttcaattt ttgcatagtc ctcatctaag aatacctcat
    17221 cctcctttgt ttctgttgac tcagcagcta catccagggt cgtcatatga tagactgact
    17281 ggaaatatgg tccaaatagc aaactgacag ttactaactg caaaagcttc aattttaacc
    17341 ctttcacatc cttatactcc tttcaaaaat atttgtcttc tttcacaaca atcaacaaaa
    17401 caatgcccat ctaattataa tttaatttaa ttttaatttt aaatcaaatg atatcctttt
    17461 tagttaaaaa acaccaatta tacaacattt attattaaaa ttaaattata aaattttata
    17521 acgttctcat ttaacaaaac ttaagttatt aagacaaatg aataaacaac tacgctttcc
    17581 tcgaaacaaa aaaataaagc acaaatgcgc atttacgcac tcatgcttta ttcgtacaat
    17641 taattcagat aacggcagcg cataggatgc caataacact tcctaaaata gctcctccta
    17701 aaatgtcagt aggataatgt aaaagtaaaa aaatcctcga ataagcgatc aaacaagcaa
    17761 ataaaaaggc taatattcct gcaactggac ttaattcgaa caacgcagct actgataaaa
    17821 aggctagcgc tgtatgtcct gaaggaaaag aagaagtctt cggtttactt gtgtaaagga
    17881 caagccgacg atttttttca taaggtcttt tccgtcttgt cgtttttttg ataaaaagca
    17941 aaccaacaaa aaaatagatc aaactgccga taagaatggc tagaccatat tctctagtat
    18001 caggtatcaa gactaaaaca gcagcaagga gcaaccaaat aaaaccacca ttagagatat
    18061 tactgataaa tttcaaaaaa ttgatttttc gactatccgt tttccgttgc ttggcgaact
    18121 tgttgataat actatcatcg attttagcaa tcaaacgcaa catgttattc ctccttttta
    18181 agaacctttt cttgcgtacg atggataaaa agcaggactt gaaaataaaa caagaaactg
    18241 aaacctaata aagcaaaata gattggtcct gcaaaccaga atgcgacaag aaggatcaac
    18301 gtgcctgcca aatagaaaag cgcgcttttg aaatcattaa aaaacaagaa aaatgcagta
    18361 atccaagaag cttctttttg ttccagttga agcaagattt tgtaactaaa gagaagcgcg
    18421 cttaatagaa gcaaaaacaa cgtagtatac atcaatgtat agccaattcc cccaccccaa
    18481 tagcggaaaa tctgataatc gatcaataga agaagaaaat aaacacttgc taaactatct
    18541 tgctttccat aaagagagaa gttctcacga aactttttcc agaatagtga accgagcttt
    18601 ttctcttcat tttttttgta atgataaatc atatacagaa ctgttcgact tgcagttcct
    18661 aatgtgaaaa caggcaagac gcacaggaac catagtagct gtaaatggat aagacgtgga
    18721 atccattcaa tgatttctaa gaaataatca ttggcttttt taccaatcga tcgattcatc
    18781 atccttttga acctcctgtc aaatccatcc cttcaatgaa ataacgttgt gcaaaaaaga
    18841 acagcaaaat cgtcgggatc aagacaagtg ttgctcctgc catcagataa ttccattgtg
    18901 tcgttgcttg gttctgaaaa acttgcaagc caatctgtaa cgtgtatttt tcttgatctt
    18961 ggatatataa cagaggacct aagaaatcat tccaagctgc gttgaaggaa ttgatcgcaa
    19021 tggtaatcaa ggcaggtttc gtcaatggaa gcatcaaatg actccaaata tacaaatgat
    19081 tcgctccgtc gatttcagca gcttcgatca attcattatt gatcgataag taaaattgac
    19141 gcatcagaaa tatattgaac gcattgccga agaacgatgg aacgatcagg ggcaaataag
    19201 tacctaccca gccgatcttg gagaataata catattgagg gatcatcgtt acaaaccccg
    19261 ggatcatcat cgtagaaaga accaaagcaa acatcagctt tttcccagga aaatcaagct
    19321 tcgcaaatcc ataagcaata aaagaatttg aaaggacatt accgatcact actagcacag
    19381 tgatgaataa tgtatttctt gcatagcgcc aaaaaggaaa ggcggcaatc gtatccagat
    19441 agttttccca tacgattttt tctgggaaaa aggtcggagg ataacgaacg atttcttcca
    19501 ttggtttcaa tgaagtaaac accatccaaa gcaatggaat gatcaaaatg attcctatta
    19561 ctgtcatgag gacataactt agtatttttt ttgtttgccg attcacactg gtctttttga
    19621 aataatccat catttgccgt cacctccaga ataatagacc cacttaggtg caagcttcaa
    19681 attgacaaac gtcagaacca acacgatcag aaacaggatc attgacatag ccatcgcata
    19741 acccatatca aaagctacga acgccttgtt ccacatatgc agattataaa aaagcaaaga
    19801 atttgcaggt ccgcctgttc catttttcgt catgacataa gcttcttgaa aaatttggaa
    19861 agaaccgatc gtagaggtga tcacatcgta aaaaataata ggagtgatta atggcaaagt
    19921 aatacttttg aactttcccc aaacgcctgc tccatcgatt tctgctgatt catataaaga
    19981 tctaggaaca ttttgtaatg tagctaagta aagtagcatt gctcctccag aactccatat
    20041 tttcatcaaa atcaacgcag gtttcgtcca ttgtggatca aacagccaag ctggtccagg
    20101 gatatggaac cagcccagaa atgtgttgat caatcctgca gatggagaaa gcagctgcat
    20161 ccataagatg tagactgcta ctcctgataa gactgccggt aaatagaaag ctgtacggaa
    20221 tacactgatt cctttgattt tttgattgag tactgtggct aaaaacacac tgaaaatcgt
    20281 cgtcagcggt acattgaaga tgacgaaata aacggtgttg tataacgatt tccaaaataa
    20341 atcatcgtga acgaacattc ttttgaaatt tgctagacca atgaaattca tcttggatgt
    20401 caaattataa tcggtgaaac taccaataac tgaaaaaagc ataggcccta gcataaatac
    20461 caagaacccg ataataaaag gcgagataaa taaataggcc catcttgctt cttttttctt
    20521 tcggatagat aaacgcttca aattttcttg cctcctctgt acctagacag tcaaaaatgg
    20581 attattgatt ttgcttgact aagttttcta ctgctttttg tgcatctgcc aatccttctt
    20641 ttggtgattt tgttcctaaa agaatctggt caatctgttc attcactaat gtcgtgtaat
    20701 ccggagcatc taaaggaact ggtgtgatca ccgtattttt aaagttttcg tccgccattt
    20761 gatagatcat ttttccttta tccgttaggt tgtcattatt cactaaattc tcattggctg
    20821 tcttgctcgc catgatatca aaactttttt cgccgaattt ttcttgtact tctggagaag
    20881 aaagatattt gatgaattca taagaggcct caggatcttt tgctccgtat ggtacttcca
    20941 agacgaatcc gccaccccac gaccaatggc ctgaaccttc ttctttttct gggagctgca
    21001 tcacgccaaa atcaaaatca tccggtgcat tttccatcaa actagagtaa taattgatat
    21061 tttgggcacg catggctact aatccggaaa taaatggatc agatacacca gacccaaatt
    21121 cggcttctaa tttattgatc gtatttttcc cataataatc ttgccagtct tggatccact
    21181 ccaaggcttc cactttattt ttagtatcaa tcttgacttt ttcatctttg tcaaaccaac
    21241 tagtcccgtt atctgcgttt agcgcccata catcagcccc taaattccat aatggataaa
    21301 aaccgatgcg tgtaaattct tttccttctt ttttatctag tttacgtgct gtttcttcta
    21361 attcatccca agttgtcggt agatcttgtt cactgattcc agcttcttta aataactttt
    21421 tattataaaa aatgacttgt gtatctgtat tgaatgggat cgcataagga tcaccttcat
    21481 attctactgt atcccaaagc tgcggataaa aatccttaga agtgtctttt tctacatatt
    21541 tggagagatt agttgcttgc tgtgcttctg ctctttgggc aacggaattt atgtcctgta
    21601 caataacatc gggtgcattt ccagctgtta ttgcagataa agatttagtc cagatgtcac
    21661 cccatggctg atacttataa tttacatgga tctcgttttg tgattgattg aaatcctcaa
    21721 tgatttcttc gatagcttct tttcgtgcac cggatcccca aaaggaccag aaatcaatat
    21781 tttttcttcc atcacttgtt ttttcagaag aatttgctgc acttgaactg gcatcgcttg
    21841 cattacttga tccgcatcct cctaataaaa acaatgctga acttgccagc aaccctgtaa
    21901 acaataattt tttcatattt tacctccaaa ttttaatgta accgatgccg gttttaatat
    21961 aacgatatca ttttgttctg aaaataacaa tttataaggt atgattctaa aaaaattatt
    22021 aatgatacca atactttata taattcgcga gcgctttctt tgaatttttt aatctttcgt
    22081 agcagaatga gaatgtatta aaaatagatt aggaggaatt ttgatgttta gacaccagaa
    22141 agaattgcag tttgaagcaa aaccagatcg tccgaatcca ttaatcgcga aatatctgca
    22201 ggagctgatc ggcggccaat atggcgagat gagtgtagct atgcagtacc tgttccaagg
    22261 ctggagctgc cgaggagaag aaaaatataa agatatgctg atggacattg caacagaaga
    22321 aatcggccac gtggaaatgt tggcgacaat ggttgctcgt ttattagagg gtgcgcctgt
    22381 tgaagatcaa gaagcagcga tgcaaaaaga tccaacaatc ggtgccatta tctctggaat
    22441 gaatccacag cacgcgatcg tagatggctt aggacctcgt cctgctgaca gcgtgggcaa
    22501 tccatggagc ggtgcctata tcattgcgag tggcaacttg ttagcagact ttagagctaa
    22561 tttaaatgcc gaatcacaag gaagacttca agtagcacgt atttacgaaa tgatcgatga
    22621 tcgtggcgta aaagatatgc tgtctgttct ccttgcaaga gatagctatc atcaaaatct
    22681 atgggaatgt gcagtgaaag aattagaaga aaaagaaggt tcaatcttag tcccaagtac
    22741 tttcccgcgt gacaaagaac gaaaagacat tgcttatgac ttctataact tctcagaggg
    22801 agaccaaagt agtgaaggtt cttgggctag tggcaaagca ctggatggcg aaggcgaata
    22861 tcaatggttg aaagaaccta agatcgaagg cgctgcacca aaattagaac cagtaacacc
    22921 aaaaatgtat gggacacctc cattcaagaa ataaatatct acccgtagaa acacccgttt
    22981 tttgttccac acgctttgtg agcaaaactg actaaagtca ttaatcataa ccacccttca
    23041 aaagggtggt tttctctaga gctataagct caaatcaccg gccagcgcct aaagacgctg
    23101 gctttcatat tttatgttca agccatattg cctttgccag ttttgcccac ctcttaaaag
    23161 gtttatgtac tactccttag cccttaaaag gatcgtctta ctctcgtaca ctcagcttat
    23221 ctaacgcact atcatgtttt tcttgttctt gaatgtactt tttgatggtc gcttcatttt
    23281 gtcctacttt tgtcacatag tatccttcca cccaaaagtg tcgataccca aatttatatt
    23341 ttagatttgc acaatctctt gatgatctcc tgaatattgg ctcgatattg attataaatt
    23401 atttttcttc tatactttgg tgtgaataca atgtggtatt tacacatcta ctttgtacgg
    23461 gctaaactat ttccctttat tgctccaccc gcatagcaag tggttttgta tttcaatcgc
    23521 tcggcgcgct caattaggtc taaagacaat aaatctaaaa acccgctgct tcactgacag
    23581 aaacaacggg ttttcagaaa aaaatgaaat gaaaaagatc tcttacggca atctatttta
    23641 tttttttgat tttttataaa tggaaaatgc ttcaagaagt ccaagaaata aagccgtata
    23701 aaaacaagaa cctatgaaat ctttatttat tagatattca acagaaatcc cgataacact
    23761 agccaacaat atatttatca gaagtgtaaa ataaggatgc tttttataga atagaactag
    23821 tgttgttttc atagactctc ctttcttcat atttccaatt aatcacaaat tcacggcatt
    23881 actagaaaaa ttaaaggggc gcagaagtgg aaaaatactc ttcgactttc cctacaaacc
    23941 aggccatctt gtgtttaata actataccaa atctaaggta cctctaaatc taaattccat
    24001 ttgtggcggt gacctccaat ttttaactct tctatctccc atccagtata cataaccttg
    24061 atacttcgac cacttatata tcgaacacac aaatacctat ccattgcagg agtactacct
    24121 aacttagttg ctacttgcat ctaaataaca gctgaaaaga taggacttac tcttagtaaa
    24181 gtatctgaac tattaatttt taaactatca tttttatcct caactacatg cacatctgtt
    24241 gcaacttcac tttctgtctc atccagataa atgattaagg aatcgggaat tgcaatatct
    24301 tcagctgacg ctggaatagt tgagctaaat tagtcatcat atctttatta taaaataacc
    24361 aacagcaaaa aatacaatga aaaacaaact tggaactaat gtatcattaa taaatgttga
    24421 aaaattcagc aactcattac tgtatgattt tagcagccca accaacatat accctataaa
    24481 agctaataag atggttagga atcctttaga atttttactc attcatagac acttccttaa
    24541 ataactttta ttatagaagg tattgtaaat aataatattg acaaaaagtg actcaaaata
    24601 aaaaaaacct tacattatac tggaatgaac tgtattgata gtgtacctaa aagctctact
    24661 gacataagat cgtgtaatcg gtaaagtaat tttattaatt atacgggatg taatcaatta
    24721 gagaataaac taatcataaa aggtactttt acattcataa ttgtcgcttt gagattctac
    24781 tttggttcac tatctgaccc aattgcacat tttcgatcat tacaagtatt ggtgtcgtta
    24841 gaacaactaa ataaaaacta gcggtagatt tttcagaatc tatgctaatt tctgtatgga
    24901 tgttccagtg atggcaaatt tctcttttca ttgtttttct tgtcgcatga tatgcctttt
    24961 tgtgttattc agttaaacta tagaaattct ttcctagaca gatcttgatc ccaaatttta
    25021 cgtttttgag catcgatata gtttaaaatg acattactca tatctaaaga gtttagattt
    25081 tttaggacta aaactgtcag ttgttctttt tcttctttct gttaaagtat tgtcgcgact
    25141 tttcgtatac gttaagatat gtcgtgaaaa aacaagtttt gtgacacaaa aaaacgaatg
    25201 acttgaggaa atcacctcaa tcattcgttt aaaatgctat atattcttta gtttaagata
    25261 cggaaattaa tgccatcgac cacactagta gaatgaacga catttacttg gccttttttt
    25321 aatttcgtta tgtttagcat ataaaaatat tatagagttt acttacaatg cttaaaacat
    25381 atactgtata atctgatcaa ttttagccat gtcttgcaat ggtaagtttt ctattttttt
    25441 gagttttcgt tccttaaaat caagagattt taattgtgta ataattactt gtccttttat
    25501 atctagctct tcaggtaatg agtaacgtgt cggtaaattc tttatagtgg aagtaattgg
    25561 acatatgacc gaaaacattg ttttacggtt aaattcataa cgagagacga ctagtcctgg
    25621 tcgtcttttt tgaatttctt ttcctacgga gggatcaaaa tcaatccata caatatcacc
    25681 ttttttagga atataaattc tttcttctga cactataaaa tttctctccc ttcaggagtt
    25741 aaatcttccc attcgtcttt ctcataatac tcggcctctt ccccaccact aaatggatct
    25801 tcaatttttg gaactaacgt aattgttcca tcatcagaat acacaacgat atattcttgg
    25861 ttttctgaag gtttctttcc gttatcagaa ggtaaagtta ccactacaga acttccttgt
    25921 aatctagatt ttgtaacaag cattatcatc actcccttaa taaaagtata ctatataaca
    25981 ctaagtaacg caaaattccc ctttgtaaac tatctagaaa attgttttgt ttaaagtata
    26041 tcttattgga tataaataga aataaattgt agccagttca ttttttcttt aactaaaaaa
    26101 ataatccaag aaacattgaa cctatggatc caaaagctac ccaaatacta tagctaaccc
    26161 cccgctacct tcaattttgc agataaaacg ctttgggaga aaatttcaaa gacaacaggc
    26221 catcctatca tgcccgatta taaacaaaaa gctggctatt ccttttgctc tgacaaattt
    26281 caattgggaa agttcacgaa actagcaggt gaagaagttt caactgttcg gatcaaagaa
    26341 tgcccgcttc aaatagaaac attggttcaa cgaatttcaa ctagacaaga ctttgctatt
    26401 gtagaacgtg agatcgtagc tatttttgta gaagaaggga ttttacatga tccaactcat
    26461 atcaatgtgg ataaatggca acctcttatc tataaattca gagaatatac aactgccaac
    26521 cagtcgttaa ctttgaattt tagattccaa gaattcaaag agtcataatg ataaacatcg
    26581 ttaggtataa ataaaaaaga ctcgcagcac gagtaaaaaa caaatagaag ctatacatta
    26641 gaaaggtcga ctgtgaccct atagatgcag tatatgcagc ttatgagcat ttcttctgtt
    26701 ctctttgttt ttgggatctg tgctgaagtc tttttcgttt tttgccgctc tttctttgaa
    26761 aaatcgatta tgttatacct tctccattac taattcaggc atctgctgag taatgatttt
    26821 tcgcgcaaaa gaaatatctg ttacccaagg ttcgatttgt tcttcagtca agacaagcgg
    26881 cattcggtca tggattggtg cgactgtgcg gttaggttca gtagtcatta tgattgattc
    26941 tgccattttt tcatcctgat ggctacgata aaaaccgcca atatacacta ttttttcctt
    27001 atttgaaaac ataaatttgt ctttttcttc attccactca ttgggaaaac acaacgattt
    27061 tgcaaaaatg gttgatgaaa ggtctttttt tcttctactg tctctgcacg ggcattaatc
    27121 atcaattgtc cttttttaaa tccggtaaat ccccacttca tgatttccgg agtgaggatg
    27181 ttttcttcct cttttcttaa cgtcacaaca agatttgacg gaaaaacttc tccagctgct
    27241 aattctgctg cctgcttcgg ttgtttcgtc tgggcttctc gataataatt ctgaaggagc
    27301 tctgactcta gatcaaaaaa ataacgacca cacatcctaa tccctcccaa tctttttttc
    27361 tatcatacac tgcaacaaga agttcgcacc aacaaaacgc tttcaatttt tgtagaacta
    27421 aaaaagttgt gacaagtgac aaactctcca gtgaatggta ttctcctgcc aactcctcgg
    27481 tcacaagtca caatattcaa caaacatgag aagctgcttt ttgaaattgt tccttgtgac
    27541 tcggagtgaa acggcaggct tacgacctct gggtaaacgg tgttcaaaag ccagtttctc
    27601 ggtattaagc caaatttatg caaaaatgta gaaagctatt ttcacaaaat ttttcttaat
    27661 actcgaaact aagcgacttt ttcacaacct cttccttaaa cggtgttcaa aagcttgcat
    27721 ctgcggtaat aagcccaacc aaacgaaaaa tatgagcagc tatttttatt tggctgttct
    27781 tattacttga tgcagggcag ctttttcaca acctctccac taaacggtgt tcaatcagct
    27841 aaccttcggt attagctcaa aaaacggcaa aatatgagga actattttgc cgtttgtttc
    27901 tctaatactc aggtcaaagc gctgatttca caacctctaa tacacggtgt tccagaagta
    27961 gcttcttcga aaaataagcc gaaatttcac aaaaatttga gaagcaattt tcgtaaattc
    28021 cttcttattt cttgaagctg accacttctg tcacaacctc taattattaa agaacctttt
    28081 gcgaagttaa caatctttct ttgatcaatt gaaaattctg cctaagtagt gatagatcct
    28141 gtgtgacttt caacaataag atacttccct ggatttcagc aatcaacgct ttggcttgac
    28201 ggtctgcttc ttcatcggac agtttttctt cagaaagtgc caggatattt tgtttcgtat
    28261 acttgaccca ttgattgact aattctagta gcaacagacg gtattcttca tccactgtac
    28321 tcaactcaat aatcaaatta cctacaggac agccatgata agtgatctgt gtctcatgat
    28381 cagcgaaaat ccagtccaac atttcttcta atgcttcttc tggctgtttt ccagcattta
    28441 agatgtcttc gaacaattct gtctgccata cgtccatatg cgcatggatc accgctagac
    28501 aaacttcttt ttttgaagaa aagtaataat aaaattgacc ttttcctgta tcagaagctg
    28561 ctagaatatc tcggattgac gtttcatgat agcctttttt ataaataaga tctttggtcg
    28621 cttccaagat cttttccttt gttttcaatg atattctcct ttttcatttc tcatagtcct
    28681 atattataca gtaaattcat cattttttct tcttaatcgt ttaaaaaaga ctgtgccaaa
    28741 ttatctgaca cagccctcac tataaaactg ttctcatata gctagatgtt cattgattca
    28801 agaactgcga ggacaaaagc aattcctatc ttatgatagt tgctctaata cttcctctgt
    28861 tgtcatcact tgatctgcaa taaagccaaa gttcgtcaac gctgcatcca ttgcttcttg
    28921 gccaggagca ccaattgcat ctgtcgctac cacaacattg aatccactct ctaccaattc
    28981 acgcatatgg gaatcgctac acaagtttcc gttcattcca ccaagaatta ctgtgtcgat
    29041 tccatttttt ctcaattgga gcgccaaatc attgctttct ggtccaaaga ttttatgagg
    29101 agagctgatg atcgtatttt catccaaata aggtttcaat tcttcgataa aatcggctcc
    29161 tgaaccttct tccactgatt catattgact ttttctagca aacatctgct gctcaagcat
    29221 catatgttct cctgcaccgc caaattgcca tttatggtca tgtgggtaaa aataatgagg
    29281 agaaataaag agtttgtatc ctttttcttt tgcttgtttt accatttcaa tcatattttc
    29341 gatcgtattg tatttttcta agatatcttt tgtcaaacca aagccaccgc ctgctggttt
    29401 caagaactcg acttgcgggt cagtgagaac tactgctgta tttttattat tgaacatggt
    29461 ggattctcca ttcgttattt attttaatac atcatttaga gcttcactca ttgtcggatg
    29521 agaatagatt tgatctctca ataacgtata aggaagtttt ccattcatag ctagcgaaat
    29581 caagttgatg acttcatgag aatcttccgc atagatactt gcccctagaa tcaagtcagt
    29641 ttcagggtcg actaaaactt tgaatacacc tttaggatct tctaatactt gtgctttagg
    29701 gacaccgctt gtcatatact tgaataattt gtaattgacg ccttgtgctt tggcttgttt
    29761 ttctgttaaa cctacattgg ataaagtcgg agtgataaat acactgtaag gcacagcttt
    29821 ccggttattc gttgttcgtt tattctcgcc cttcaactga tccataatga ttcgataatc
    29881 atctagagag atataagtga actgcaatcc gcctttgacg tctccaatcg cccaaatgtt
    29941 gtctgcatct gtacgaaggt attcatccac agcgattgct ccgcgatctg ttagtttaac
    30001 atctgtattt tctaatccaa gtgatgctgt acttggtttt cttccagttg cagccaaaac
    30061 ttttgacgct tggattgtta cttcttggcc atcttctgta aaggtgacgg cagctgttga
    30121 ttcttggtct gctacttgat ctacagatac acctagatgg aattcgattc ccgcatttgt
    30181 catgtcttct aaaatcattt gcgcgatatc ttcatcttca cgtggaagaa attcagaatg
    30241 ggcatcaaga acgacaactt tagatccgta ttcattgaac attgatgcaa attccaagcc
    30301 aatatatcca gccccgatga tcactaattt ttccggtaat tgttcctgat ccatcgcttg
    30361 cgtagaatca atcaagtagc gacttgtatt caatcctttg attggtaaaa tgattggttc
    30421 agaaccagta ttgatgaaaa tccgttcgcc ttcaacaatc actttttcac cattattcgt
    30481 cacttcgatt tgatggttag aaagaaaacg tgctgtaccg tccatcactg tcccagttgc
    30541 ctcatcgctg atcatatgat aatttttggc gcgaagcatg cccgtcaatt tttctttccg
    30601 agcaacagcc tctgtaaagt cgattcctct ttctccattg aaaatcaatg atttagaagg
    30661 aatacagccg atgttgatac atgttccgcc atacatacgt gttgactttt caacgactaa
    30721 aaccgattca ccttttgaag ctaacgcctt cgctaaagtc ttaccgccct ttccaaaacc
    30781 gatcacaata ttttgatatt tttccatcat ttttccctcg cttctttttt acctaccgat
    30841 tggtacaaaa atatgataac cgataattgt ctattctcca aatcatctgc tttatttgtc
    30901 aaaaaaaacc agacaaaaag cggtcctttc acgtttttag tctggttcga tttcctttat
    30961 tcttctaaac aatcgttact tctacatcga tatttcctct agttgctttg gagtaagggc
    31021 agaattcatg cgcatcttct gcaatttttt gggcttcttc ttcgctgatg ccatcgatat
    31081 gcacttctaa tactacaccg atctgatagc cgccatcatc taaattataa agagagacac
    31141 gtgcttttac tgtggactct gctgtcattc ctgcttcttt gatcatatgt tgtaccgccc
    31201 cattgaaaca agaagcataa cctgctgcaa ataattgttc tggatttgtt ccttctgcat
    31261 ggattcctgg aggagtgatt ttcatatgca tcgagccatt tggtgcctcg acttctccat
    31321 tgcgcccgcc atggttgatc attgctgttt cgtatgcttt tttcattgta ctgctccttt
    31381 cgattgacca tttgctactt ggctataatt atagtgtatg acaagtaagg cgctttctac
    31441 aaaaattctg cttattcgaa taccagaatg ccgtcttcta ctatttttcc aaaaaacaaa
    31501 aaaatactac tcgtgaacaa cagcgtcata tttgttccaa aataaatagc aatcttttct
    31561 acataatctt ttatatgatt gattttaaga tacatatata ccagaaagtt gaccccataa
    31621 accaatactg ttaaaaaaat aaccatattc gttaccattt tttcctcctt ttgctggtct
    31681 ccctaccagc actcgattta accataccac tttttttgcc gtccaagcaa ctagcctttc
    31741 aaaaagactg gtttattctg ctgttaatga ttcaagatca gaaaaatgtc cgaataattt
    31801 tgctaatttt actaacgcca acttgttctt tccgaattca tcattttcta tctgtaatac
    31861 tagtaacggc tcatgtaagc ttaaacggat caaaaaccag ccgtttccgt atggctcttc
    31921 tagatcatat cgaatacctt cttcattttc cgggtcgaag ttcatccctt tttcttcttc
    31981 caacagtcgc ttcatttctt ctaatatctt gtttccttgt tcgcgtggtt catcgctgaa
    32041 caattttaat cggatttctt gtacttctac gggctgtttc aactctttga tttgttcatc
    32101 taatgtgata ccttttcttt ttaattctgg caaaagcatc aggatctttg cgatgacata
    32161 tgcaccatca tccagaaaat agttttcctt gaatgcagcg tgtccgcttg tttcgatggc
    32221 tagctgtgta tctttcccaa gacggttaag ttctaatgct ttattgatta catttcgata
    32281 gccggaaata taacgaactt ggatgcctcc tttttcttca atgaatcttt gtagatgaga
    32341 agaagtaggg gaattcgtca cgattcttgt tcctggatgc tcttttaata cgattgtact
    32401 taatactgcg atcaaattat ttcgattgat cactgttcca gaagcactga caactgcaga
    32461 acgatccaca tctgtatcga aaatgatccc aagatctgct tcatttgcta aaacagcctg
    32521 cttgatactt tccatcgctt ctttattatc aggatttggg atatgattag gaaatcggcc
    32581 atctggttcc aaaaattggg aacctgttga atccgcacct aagctattca atactttttc
    32641 agcaaagaat ccaccagcac catttccggc atcaacaatg attttccaac catttagtgg
    32701 tttgctttct agtgttccaa tccccttttt aatttttgtg actaaatctt gcgcataagg
    32761 tgtcaaaagg tctgcttttt ctacgcttcc agtttgttcg ctttttattt tttgctgctg
    32821 tgataggata aaagcaatat cttctttttc tgctcctcct tctgaggtaa agaattttat
    32881 tccattataa taatagggaa gatggctagc agtcagcata accgcagcat cgcacgagaa
    32941 ctgttcaaat tgagtggaca taaacatagc aggcgtagta gctaattcga aatcgatgac
    33001 atgtatccct tgttctttca gactttggat caacgcctct ttcagtgctg gtccgcttat
    33061 cctactgtca tgaccaatcc caatcgtcca ttttttattt ggatcggttg atttcttctt
    33121 tttctttatc cagtttgcta gtccccagcc gatctttttt accatgtctg tcgtcaagtt
    33181 tacttcatat ccttcaactg gtaatgcgat tcctcgaata tccgatccat tttgcagcgc
    33241 tttcatatct ttcatataaa agtcctcctc atttattccc tcattttttc cagcttttct
    33301 gcaaatcgct gtgcttcttc ttctgagcgg caaataataa tgaatgtatc tacaccggcc
    33361 atcgttccga caacttcagg gtaagccact tcatctagcc aattcgttac cacatcggca
    33421 ccatttcgtt cagtcaatac gataaccatg aactgaacag actggatacg caaaacctcg
    33481 cgtctaattg ctgtacgcaa acgttcttca tttaaagaaa cagatggctg gttaaataat
    33541 gtatagcgta ctattttgtt ttcattttga ctttttacta acttcatttc tcgaatgtct
    33601 cgagaaatcg ttgcttgagt cacatcggcg cctgcttcat gcagccgcgc aagcagctct
    33661 tcctgtgtcc caataggttg ttctgtaatg atctggcgga tcagtgcttg tcgctgtttc
    33721 tttttcatat tagacacctc tcaatgttga ttatagcaaa agataagtaa tatttcttca
    33781 tattttggta ttttttactt ttattttttg ttttcattca ggttgctaac ctttttctat
    33841 tttaacacgc aaaatcgtct tcattttttc aggtgctgat tttcttggat cgctgatgta
    33901 aatctctttg tgttctttag aagctctttc atagccttct tttgcgatga atgcttctaa
    33961 tttctcaaac gttgctggtt cttcggcata gggcccgacg tgtaagattt gggcacaaag
    34021 accttcttcc atctttttga atactacttg ttctttcaag tcttccggaa gtttgctaga
    34081 acgctgaatg gcttcttgtg ctatcgcttc tgtaacaaag tcaggctgtc ggatcatcaa
    34141 ttgataagaa aaatgttctt ttttcatcac tggttcatgc aaatactttt cttgcagtcc
    34201 ccattgtcct tccagtggat agacagtata catctgataa tcaggaatca gccaattttt
    34261 tttaggcgac atgcgtattg catagctgac tgcatataga caggctactc ttcgtttaaa
    34321 gtcttcctga ttcgggtcgc cgatccctct gatgctaaag aatgattttt taggaacctc
    34381 tagtatttgc gggctttttt tcatactata gctcttgtct ttacgccatt caattttttc
    34441 catttttctt cctccgcgaa attccattta gttatttatt atcatacctc tttttgagtg
    34501 acttggctaa tcgtgttgac actatttttc agtttgtgct agaataagtg aagtaaataa
    34561 atcgtacaga taacacgttt cggcgtgtcg agagagaggc ggtttctatt tttataatag
    34621 aaatcactct ctttttttac gaaatttttt agaggtgatg taaatgacaa aacaaaaaca
    34681 gctgctttta gtacctgcag taattgcaac agggatgatg tcttttgcgg gcgtattgat
    34741 cgagacggcc atgaatgtaa ctttcccaac tcttatcaga cagtttgatt taacgactgc
    34801 acaagtccaa tgggtaacaa cgatttatct tttgatgatt tcgatcattg ttccgctgtc
    34861 tacttatttg aatcgcaatt ttagtctcaa aaaactcttt ttagcttcaa atctgctttt
    34921 cttggcaggt ataatgatcg atttcttctc tcctaccttc atggtcctat tgtttggtcg
    34981 attgctccaa ggtgcagcga caggtatcgc tcttccgttg atgttccata ttatcttgac
    35041 atttgccccc ctcaatcgac gaggcgcgat gatgggtgtc ggtactttga caacggccat
    35101 tgctccagca attggcccca cttatggagg cattatgact tcacatttta catggaatca
    35161 tatcttcctg tttcttgtgc ccgttttgtt tctttctttg attatcggct ggtttgctat
    35221 tcccgagatg gcagtacaaa aaagtggtta tttggattgg ataagcgttt taggaatttg
    35281 tcttttgttt agtggaagtc taacattttt gagtacgttg ggaacagttt ccagctggat
    35341 tgctttaatc atcggcggta ttggctggtt ccttttttac tatcgaagta aaaaagtgga
    35401 gcatccactg atccgactgt ctattctttc aaataaaaca ttccgcttgt ttttgattgg
    35461 cttcttagtt tatcagtttt tattacttgg tgtatctttt gtcttgccga atttccttca
    35521 gatcgttcaa ggaagtagtc cttttgtcgc tggattagcg atgcttcccg gcgcagcggt
    35581 tggtgctttg ctttctcctt tttccgggaa aatgctggat caatatggtc cgaagaagcc
    35641 aatttttgct gggctgattc tttctttgat tggttggcta gcattattcc tactcatagg
    35701 acacgcttca gtgggtgtat tgattgcttg tcatgtgttt tatatgatcg gtatcggatt
    35761 atcttacagc aatatgatga ccaccggaat gaatgcactg aatgaagaac tacaaggaga
    35821 cgggaacgca atgttcaata ccttgcagca gttttctggc gctgtggcta cttcattagt
    35881 agcggtcatt atcaattacg tccagcatca tacttcccat aactatgaag tgtctactac
    35941 attaggatca aaagcagcat taggcgtctt gttgctgttg cttctcgtaa gctttgctcg
    36001 attcgcctat tatctgttct ttgctaaaaa agcgtagatt ccatatacgg caggtgttgc
    36061 atctgccgtt tttttgtgtg aaaatggttg tttgtaaaag gaggcttttt tatggaaaaa
    36121 attgccatta tcggtggcgg gatcattggt atgaccctag ccagttactt agatacatca
    36181 aaattcaatg ttgctttatt tgatgaaggg acaggacagg caactaaagc tagcgctggg
    36241 attatctccc cttggttgtc gaaacgcaga aacaaacaat ggtatcgtct ggctaaagac
    36301 ggcgctgctt ttttccctaa acttataaaa gatttccaac ttgatcaaac gatttacgca
    36361 caatcaggga cacttttgct tcgacctgaa aaagatttag ctgaattggc tgttcttgcc
    36421 gagaaacgaa aagaagaagc gccagaaatt ggaaacatcg aatggctttc acctgaacag
    36481 acatctcaag cacttccgtt attgaaacca gcagtttcac tgcgtatcag tggtggtggt
    36541 cgtctggatg gaaaagcata tttagaacat cttggtcagc gtttagcaca aaaacaaatg
    36601 gagcagatcc aggcaaaagt cactttgcat aagaagagcg attcttggct catcaaggga
    36661 ccgtttggag aatacacagc tgatcgtgta attttgacac ctggcccggc acttaagcct
    36721 ttattacaag aattaggcta tgctgcagat atacgtccgc aaaaagggca gctcttagca
    36781 tttcagacat cttataagat gagtcaagac tggcctgttg ctatgcttga cggagaagct
    36841 gatttcattc ctttcatgga tggaaagatc cttttaggtg ctacccatga aaacgatcaa
    36901 ggctgggatt tggctcctac tagcgaagct tataagggat tgactaagag tacagaacag
    36961 ttcttgactg atccagatca gataaaaaac tggccatacg agttccgtgt aggtacccgt
    37021 gcttatacgt cagatttttc cccatttttc ggcgtactgc cagatgatcc aagcttactg
    37081 gctgctagcg gtctaggctc ttctggcttg accacaggtc cttatatcgg ctatctatta
    37141 gccaacttct taaatactgg tcattggcct acggaagatt atcaaaagga catctccacc
    37201 tatattacga aaaatccatc atagaaaaac agctttttca agtaaggatc ccgagcgata
    37261 ttgcgcatgg attacttctt gaaaaagctg ttattttagt tcaatttctg aatcaaattc
    37321 tccaaacttt cttttgcttc atctggcaag agatcttttc ctatctgagc ttcaatgaat
    37381 cgtaaacgaa tatcaagtcg ttgtttcacc agatttttat aggacgtatc ctcaaaagaa
    37441 acaggaaaat ctggtatagg taaatagtgg atgtcttcaa atggaccaaa cgacttgaat
    37501 tctgccgttt gttgcacgat tctttcaacg attgacaatg tcgtttcagg tttgattttt
    37561 ttatcatcga aatatccagt attcaggaca tagcaggctg tttgctgttt caaaaacaac
    37621 tctcgaaaag cttgatagtc ttccctaaga ggatagacac ggaaaggatt agcaaatggt
    37681 tcaatgacta gctcatctag ctgtcttttt gtagtgatat tttccgcgtc tgatcgtttt
    37741 gttgctaatg ttgcaccaaa agacgctgct aaaactgggt cattgatctt gataattggc
    37801 ggaagcgaat catctttcat gatccaaaaa acagcatcga tcttttcgct aagatgatcg
    37861 actcggtcgg gtgtcgcata acgtgatttg actgtccgcc catttccgtt tcgtatatct
    37921 cctgttacta ggactttttc tcctttactt gtacatgtca cgcccacatt ctgacaagta
    37981 aggaaagaag aaatggccgg atcatcagac ggatagtctt gtgttttatc aaagtaagaa
    38041 ggttccaagg cagtagcaaa accatttttt cgagaaataa cgaaagcatc gtcatgcaag
    38101 atcgaaacag agtgaccttc cgtatgtttt gctaaggtaa tcgttgattt tccagaaccg
    38161 gacaacccaa atacagccat cgtataggtc tggtcattac tagcatattg tttcatccca
    38221 ccgtggcagg caataaagcc attgcgattt cccaatgccc aagcaagcgt caaagtcgct
    38281 ttttttaatt ctccaaagta acgtaatcct agcacagccg ctgtattatg gagagcatca
    38341 aacaaaatga gtccatttgg aaatgtttca tgtgtccatt cgggatcaca aaagatataa
    38401 agatctccct catcaaagga aacagacttt tgataagtct ctaagatctt cgaattcgct
    38461 ggctgaaaat tcagcaaata agaatacaaa ttgttctcaa atccttctgg aatcagcaaa
    38521 tgactttgca cagaaaattc ttcagatagg ccaacgatta cttttgtatg gtaaaactgg
    38581 cgcttgcgac tttgatacac agcttctctt aaaattttct cgtatttgct ttgttccttg
    38641 tctaagtcct caaagatccg ccgagcactt gctgttcttc ccacaatgct tccatcattg
    38701 aatactagtg tcctaacatc agctggtaaa tctagttctt cagcatgtga gattgttaaa
    38761 tctgtgatga tcgttcctgg actccctttt gctagttgat aagctgtctt cctatcaaaa
    38821 acttcatgaa cattattacc gtaaaatgtc gtttctacaa tcgatcgaat actggataac
    38881 agtggatttc cacttgtgat ttcgtttaat tcgagactct cttttgtact catggctcct
    38941 cctcattccc taaacaaaaa aacgttttct ttattataca ttattcgagt ttttatttgt
    39001 actgctgcgc tgtgggaagc aaacgagtta tttttcacac aaaaaaaccg agaacttgaa
    39061 taaacaagtt tctcggtttc atcattttcc ttttatcggc ttagtaccag ccgtttgcta
    39121 accagaatgt tttagcagca tcccatgaac cataacgaga tgttacatat tgttctgcta
    39181 cacgttcttg attggcagct gaatagtcgc cgtttaagta tgaagcatct aattgataac
    39241 ggccgatgta acgtccgtta gtagctgtgt atgaaccgcc tgattctttt tgtgcgatcc
    39301 attcttttgc agagcttgaa gttggcgctg ttgcagcagc tggggcagca ggagctactt
    39361 caactgtttc aacaggtgca acgtatgtat gttcttgcgt tgctgtttca gttgtagatg
    39421 ctgtattttc tgttgttttg ttttctgttg ttgctgaaga atcatttgaa gttgggattg
    39481 ttaattgttc accaacataa atgcggttga tgtttgcgat cgaatttttt tcagcaattg
    39541 catcgatcat tgagttgtct cctgcaaatt tttgtgagat cttagataat gaatcacctg
    39601 attttactgt ataaacttca tcagcgtgag catttgtagt tcctacaaac attgcagtag
    39661 caccagcagc aaaagttgta cctaaaacaa ttttcttaaa tgaattcata ttaaactcct
    39721 ttttttctcc gttttttatt ctttcgacgc tattaacttt atcatttaga aatatttcat
    39781 aggtaaaaga aacatgacat ttaatgacaa aataacgaat tatttaatat ttgtaacatt
    39841 taaacaaata tttttttgat acaaaaccat aaaagccttg ttgtataagc aaagtaatcg
    39901 tttttctatg tttttttatt tttgaatgaa aaacgcagat tctgtaacat tcactgcaat
    39961 aatccataaa aaaaagcaaa aaaatttttg atttttatca aattctctta aaaaaatgta
    40021 aaaaatacaa aaaaaccagt aattgttaca acaattactg gacaaaaact ttgaataatg
    40081 ttaactattt ttttatttta actttctccg caataatttc tgggcatgtt ccaattcgtt
    40141 tataacgact tcttctgttt ctttagctaa tatttcatct aatacatcaa gccatttttc
    40201 cggttctttt gttcctagac gtccaatagc ccaagctgct gttcctctga tgacagggcg
    40261 gacatctgtt aaacattctg aaatctcagg caatgccgtt cgatcaccta gattagctaa
    40321 agcaatcaat gcgtttctct gcaaaggttt cttcccacgc catgaaccag ctaaatgccc
    40381 aaattgctgt ttgaagtctt tattagacat cgaaagcatt ggctttaatt ttggatatat
    40441 ttcgtctact tttggttcca tctcttcatg aaaatgaaaa tcttttcctt gattgtaagg
    40501 gcagactaac tggcagatat cacatccata aatgacattt cgcatttttt tacgatattc
    40561 ttccggcatc atcccttttg tctgagtttg ataagacaaa catcgctggg cattcattct
    40621 tccgtcacca agcaatgctt ttgtcggaca agcagtcacg cagcgcatac agtcaccgca
    40681 accaaattct cctggtttat caggctcaaa tacaatatta gtaacgatct caccaagata
    40741 gacaaatgaa ccgaactctt cagtgatcaa caatccgttt cgtccaataa atcctaaacc
    40801 agctcgctgt gctacagcga catcgatcag ttctccagta tctacttgcg gagctagacg
    40861 ccattcctct tctttttgcc atttttctgc tcttgtttgg atgaattcga tcaatcggtt
    40921 caaacggtct ctgagtatat catggtaatc gatcccccaa gaagcacgag caaactgccc
    40981 gcgtttttca tccctaggca cttcttcatg gatcctagta ggatacgcaa gtgcaatgga
    41041 aatgatcgat ttcggctgat caaatgtcat ttctggataa agacgctcat cgatatttgg
    41101 atgttcaaag ccagaagtat ggccagctgc tttttgttct tccaacgatt cttttaatga
    41161 atcaaaaggt tccgccgtgg tgaacccgat tttgtcaatt cctaatcgtt tgctttcttg
    41221 tatgatttct tcttttaatg tcatggtctt cctcgcttgc tgttaacggc tgtataaccc
    41281 ataatatagt ttttcttttt tcagctggac aaactggatc tgctgatcgg caatcttctt
    41341 ttcttccatc catttttttc ttgcagcaaa tacttcccaa taatttgttg aaattaattt
    41401 tcttttgtta tctaatgcat taaaaaaatt caatgaaact ggttttgtac ttaacagttc
    41461 atggagcctt acctcttccc atggcgaaaa ctcagacaga tcgcgggaat acaatacttc
    41521 ttcatagaga tttttttgct gtgtgtacgt gaatgtttgc gggattttcc caggcctagc
    41581 aatttctact agacgattat aaaacatatt cattaatttg ctgttggcaa taaattgact
    41641 tttataatag gccgactgga tctttttgat tactccttct gctttgatga ctgaaaataa
    41701 tgcaggccat gctttgacgg ttatttcgtc gttttgttta actaatacgc caggtgtgaa
    41761 gccgactgga gaaaggaaca caaaataacc gataccggta ttttcatcta aaggctgaaa
    41821 ttcatcaaac aaccatttgc ctatgtacag ctcttcatct ggttctgttg tctcattttg
    41881 atttgctatg aataatttag ctaattctgc ttcaagaaat tgaaggtctt gatccttgtc
    41941 ggtattttcc tgctggctga tcaatcgaaa gacttcttct ttaatcgtat atttatcttt
    42001 acgtgtaaaa ttcatagcca caccttcctt ccttactcta actataacgg aatttcttca
    42061 aaaaaggtat ctgtacgacg aaaaacagca taattttttt tcatatctct tataaaaaat
    42121 gcaaggtctg ctcctcaagc tagaccttgc acttttcagc tttcttttgt ttgtgacaaa
    42181 ccttcatgga tcgtttcaat attccatttc atcatctgat agtaagaatc tccttgttct
    42241 ccttcttttg ctatggaatc agtgaacagt ttatcataga ttttcaaacc cgtttctttt
    42301 gctacccgtt ccatgcttct tgagtctaca ctggtttcca cgaataaaac cggtacttct
    42361 tttgctctta tctgatcaat gatcgctttc atttgatcag gcgtaccttg actttctgta
    42421 ttgatctccc atatataagc tgctggcaaa tcatatgctc gtgaaaaata cttgaatgcc
    42481 ccttcacttg ttactaataa ttttttgttc tcttcgatag aagcaaaact ttccttcgct
    42541 tgtgtatcta attcttttag tttttccaca tatgcttctg cattttttta tagagtgtcg
    42601 cattttctgc atctttttca gagaatatac gtgcgatttc ctccacatat tttattccgt
    42661 tagataggtc tagccaagca tggggatctg cttttgtatg ttcttcaccg ctagttaaat
    42721 aaagaggttc tacatttttg ctaactgcaa aataatcttt tccttcttct tttttagccg
    42781 tttccatcaa gttatcgaac cagctgttac ctgtttcaag attcaaaccg ttgtataaaa
    42841 taacatctgc atcgcttgcc tttttgatgt cttctggtag tacttcatat tcatgcggat
    42901 ctgttccgac tggtacgata ctgtggatat ctatttgatc tgttccgacc tcttttgcca
    42961 tgtccgccaa gatcgaattg gtagccacga ctgacaactt ttctttactt ccttcatccg
    43021 cttggtcaga agcctgcttt ccgcaaccgg ctaataccaa gaaactaact gccaagatta
    43081 aaaagagtga tttttttgtt ttcatctatc tgcttccttt ctgaaataaa attccttttg
    43141 ttggagaaaa taaaaaagca aggataaaaa atgcggtaca tgctaacacg atcgtagctc
    43201 ctgatggcca attgtaacta taactgaaaa acacaccgac aactgcactt agcataccga
    43261 ttcctgcagc tgtaatgatc attttcagta aatggttcgt tagcaagtaa gccgtggcgg
    43321 ctggtgtgat caacatggca atcaccaaga ttgttcctac tgtctgtaaa ctgactacag
    43381 ccactaatgt caaaaagaac atcaaaagat aatggatcag cgatgtgttc agcccataag
    43441 cttttgccat tgtcggatcg aatgatgtga tcttcaattc tttataaaag aaaaagacaa
    43501 atatcaagac gattccacta acgagtgctg tcagcaacaa atcgctttcc ctaacagcta
    43561 agacattccc aaataaaata tgatacaaat ctgtcgcact ctgggcatat gaaatcaaga
    43621 taatccctaa tgcaaaaaag gagctaaaga caattccaat cgctgtatca tttttcaatc
    43681 ggctccgctg agtcacgaaa ccaatcactg ctgctgttag catcccaaaa gcagtggctc
    43741 caaagatgta actaaaacca aacatataag aaatggctac acctggaaga actgcgtgag
    43801 agatagcgtc tcccatcaaa gacatccctc gtaaaataat aaaagaaccg atgactccag
    43861 aagcaagtcc tacaagcatg gaagtgacca aagcgtattg caggaattga taattcatta
    43921 atccatcaat aaaggattgg atcatttttt ctctcctcct tttactaaat gtctgatgat
    43981 ctcaccatag gccaattgaa ggttcttcga tgtaaagact tcttgtactg gaccggaagc
    44041 gatcagctgt ttatttaaaa gaataacctc atcgaaatat tcttctactt tatgaagatc
    44101 atgatggacg atcaaaatcg tttttcctga attcttcaat tcctgcaaga tgtcaaagat
    44161 ctttcgttca cttaacgcat caatccctac gaatggttca tctaaaaaga tccattctgc
    44221 tccttgggct agagctcttg caataaaaac tctctgaagc tgtccacccg atagttcgct
    44281 gatctgtctt ttggcatatt cttccatccc tacttttttc aaagcttgtc ttgcacgttc
    44341 tttttctttt ttcccaggtc tttgtccaat tcgtaaagat ggatatgttc ctaaaagtac
    44401 aacgcctaat acatcaattg gaaaggaaag atccaattca cttcgctgtt ccacgtaggc
    44461 tattttttta cggactgagt ttaccgactg tccagaaaag gtgactttac cagtagtagc
    44521 tggtatcaac tctagcaaag atttcataaa tgttgatttt cctgcaccgt ttggtccgat
    44581 aataccggta attttttgtg gatttatcgt caagttgata gttttcaaaa cagttttccc
    44641 ttgataagcg gctgaaaggt cttgaattac aatggccgta cgttccaaat cttacactcc
    44701 tttttaggtt tacctaactt tttatttaaa tatagtttaa ctaaaatgga aagcattttc
    44761 aagtagaatg atgaaaaagt ttttttcgaa ttttcctcgc attttatgaa aattttctgt
    44821 agaatcacat caagagttca ataaggctaa cctaataatg aaaaacatgt gttgaaacgt
    44881 ttgattattt tttcttgttg actcaacaac gaaaggagtt tttgtatgga agaagaaaag
    44941 ttaactcgaa gtcttagtgc ccgtcatatc caaatgatcg cactaggtgg aacgatcggt
    45001 gtcggtctgt ttatgggtgc ctcctcaacc atacgttgga cagggccctc agtgatggga
    45061 gcctatgcga ttgcaggatt ctttttgtat ttgattatgc gtgctttagg cgaaatgctt
    45121 tacgttgatc cttcgactgg ttcttttgca aattacgcga gcgagtacat ccatcctgtg
    45181 gctggctact tgaccgcttg gagtaatatt tttcaatata ttgtcgtagg aataagtgaa
    45241 gtgattgctg taggatctta tatgaattat tggtggcctg atttgccggc aattattccc
    45301 ggaatcattg tcgtcttgtt tcttatgctg gcaaatctta tttctgtcaa ggcgtttggc
    45361 gaattagaat tttggttttc gatcatcaaa gtcataacga tcattttgat gatcattgca
    45421 ggtcttgggg taatcttatt tggatttggt aatcatggtc aggcagtagg gatttcaaat
    45481 ctttggaaaa atggtggatt cttcactgga ggcgtcaaag gatttttctt tgcattatcg
    45541 atcgttgttg cttcctatca aggaatcgaa ctgatcggaa tgactgctgg tgaagcagag
    45601 aatcctaaaa agacgatcat tgaagctgtc cagtccacta tcggtcgaat cttgattttt
    45661 tatattggtg ctatttttat cattgtgagt atctatccgt ggaatcaatt gagtgagatt
    45721 ggttcacctt ttgtacaaac tttttctaaa gtcggcatta catttgctgc aggactgatt
    45781 aattttgtcg tgattacagc ggcattatca ggttgcaatt cagggatttt cagcgcaagt
    45841 cgtatggtct atacacttgc cataaatgga aagatgtcta aaaaatttct gaaactatct
    45901 cgtcacggtg cgccttttta tcctgtcgta gcaatctcag gtggaatctt aattggtctg
    45961 atcttaaatt atcttctacc aatgttcatc aaccagtcag aaaatatttt cgttttcgta
    46021 tacagctcaa gtattttgcc tggtatggtt ccttgggtag tgattttgat cagcgagatt
    46081 cgcttcaaaa agaagcacgc agataaaatg catgatcatc cttttaaaat gccactatct
    46141 ccttatacga attatgtgac acttgctttt ttagctatcg tattgatttt catgtttatc
    46201 aatcctgaaa cgagaatctc attgattatt ggactcgttt tcttgcttta catgacgatc
    46261 cattactttg tacgcgaaaa aagaaccgga aaataaaaaa agaacgaagc ttcaccttag
    46321 tggaagcttc gttctttttt attgctttta tcaattcaat tttagcgtct caccgggagt
    46381 caggaaataa ttctccatgc tcatcccatt cagcgacaag atcgtttcta ctggtacgcc
    46441 agttcgttcg gctatttgat ttggaccttc tccttccaaa actacggttg tcgcagcctg
    46501 tgaagtcgcg ctgttggtat tagtttcaga ggaatctgtt gtgtcttgtg ttgttcgagc
    46561 ttgcatttca gaagcttcta ccgactctgc tgtctcagaa tcagaagatg ttgtgtcttg
    46621 tgtgcttgtt gactctgctt gaagagaggc gctagtctgc aaataaatat cggaaatctt
    46681 agcgacatga ttcgtataat tagccgataa ttgattgatc aaagtatcta agtctacacc
    46741 gtctttattt tctgctgcct gatacgctgc acgtaatttt ctgatccctt cgtcggagat
    46801 tgcttgaagc tcacgtgctt tttggttagc aactgctgat aaaccagcta ttccctcttg
    46861 atttccctga atttctgtct ggtattcttc aatcaatgca ggcgtctttt cttcgatctt
    46921 tttcgaataa tcatttacga ttgcttgaag ttgctctggc aaaccatttg ctgttttttc
    46981 ttcagaagtt tgtgtctctt ttgttttagt tgatgactca cttgtttgtt tcgtctctgt
    47041 tttctttgtc gttgtttccg ttatggtcgt acttttcgct tctgacgaat tttttccatg
    47101 aaaatataga cccgcaactc ctcctgctat gatcaaaaac acggcaactg ctgccccaat
    47161 catgatcatc cgcttacgag ctgcctgttt ttgctgtctt cttcgggtca tccgaacatt
    47221 ttctccccac tcattttcat catacatttt tttcaccacc aatttttatg tataatagcg
    47281 ttatcaaacg cttcatattc ttattgtagc aaataattgt cgctgtgcac atttttatct
    47341 ttgttattta taagaaaatt tcaccagttg agatgattcc aagattgtcc gtcgatttct
    47401 ccttgcagct gcattgttcg ttcgtctatc tcagccatca gtttctttat ttcatataag
    47461 agcgcacggt cttgatattc tgatttttcg cttaattcat ctaattgttg gatcagccaa
    47521 tcatttgtca taatgaagtt cctttcattt cttgcagtgt aaaggtcaag tcttcgatct
    47581 gctcttttgc ctcagtcagt gcttctacga tatatgcttg tttttcagat tcttcttcct
    47641 gcattttctg attttcctgt actctttgaa taaaccagtc aatccgctga gtcagcgatg
    47701 gtttgccttc caatgagaga tactgctgat aagcaattag cttctcccaa cgtttttcct
    47761 gggataaata cataaattgt gctaaaacga atggcggaag ataggtcata tggaatttat
    47821 ttgcgattcg ctgatacggc ctcaaaaatt cggaaatcgt atattcttct tttcctccag
    47881 cttggtattc tttttccgcc acacctgttg tcaatacgat tcctagttct ttccctttta
    47941 atacatgtgc tgctcgttcc aagacattat cttgccaaat ttttaaatga gcaggagaac
    48001 tataccagta aaacggaaac tggaaaatga tccgatcatg ctgggccaag agctgtagtt
    48061 cagtctctat atctattttt ccgtctggat aacttgattc caaatgatgc caagtagctg
    48121 gtaaaccagc agaagcttct ttgaaaaatt gctgatttaa agattcttcg atcgttggat
    48181 ggctaatgat gataagtgta cgcatccctt ttcctccttt ttagtcttcg ctttacagtg
    48241 tagcataacc gccacaagtt tcttttgccg atgcttgtca ttttaaccag actaatcagg
    48301 ttgtccaaga aactgcggtt tgtttttcca actgtttttt caaggtatcc aaagcaatat
    48361 tcttaaaatt ttcgtttttc atacgtttac gatagttcgt agccgtttct ccggtactct
    48421 ttttgaacat tcggctaaat acgactagac tattgaaccc tacttgatag gcaatacttt
    48481 ccacggattc atccgtcaga gaaagaagaa acgcccctcg attgatccgt accccgatca
    48541 aatattcctt gatactggta cccatatgat ttttgaatac tcgcgacaaa tggctccgat
    48601 tgaccgctaa atgctgggcg atttcctcaa cagacaattt ttcagcataa tgtgcctcga
    48661 tgtatttgac tgtttcaaca gctaaagagg aatgaacctt ttgtacacca ccgctgacct
    48721 ttccgccatc ttttaaaagg actgataaaa aacggtaggt taatgctgtt aactgtaatt
    48781 cattttccaa ggtttcttcc gtatctttca tgcattctaa aataatgtcg atatattttt
    48841 gtatgtcaga agagaccatc gtgtattggt cttctctgaa aagcgactgc cgaatgattt
    48901 cttctgcagc actcccacca aatgaaagcc agcaatacat ccacggatct tctccatctg
    48961 ataagtaaaa cgtcgagtct cctggacgaa tcaagaacag atcacctttt ttcaattgga
    49021 attgttgatt tttcacatga taagatcctt tgccttccat cacgacatgc aggatgtaat
    49081 cattgcgaat agcaggacca aaagaatgaa acggcatcgt tttagagaaa ccacaaaaat
    49141 caaaatacaa ttctttttct acttgttgat gttccttttt taaaaaaatc gattgctcca
    49201 cgtctatccc tccttactcc tttagtataa tgcaacaaaa agttaaataa aagtcacaga
    49261 aatatattaa aaaggaagcg atttcttgtt aagtttaagt cattaaaaga tctttgttat
    49321 tcgttgtaac cggtttactt agtattatat attgaacagg agaggaatac agattatgaa
    49381 aagcaaaatg atccaacgtg tgtcctacgc tttaggcgct tttggacacg acacattcta
    49441 tgttacatta agcacttatt ttatggtatt cgtgacaagc cagatgttcg caggaacaga
    49501 taaggcaacg aatgcaaaaa tgattgctac agttacaagc ttagttgtag gtatccgctt
    49561 agttgaaatt atttttgatc ctttgattgg tggcatcatc gataatactc gtacaaaata
    49621 cggtaaattc aaaccatggt tagttatcgg cggcgttgtt agttccgtac tcttggtagc
    49681 aatctttacc aatttctttg gcttagctac tcataatacg actttattta tcatattatt
    49741 tgcaatcgtt ttcatcttat tggatagttt ttattcattt aaggatatcg ctttttggtc
    49801 aatgattcca gctttgagta cggaaacaaa ggaacgtgaa aaatttgcta cgtttgctcg
    49861 tttcggctct tctctaggtg caaatggtac tactctagtc gttgtcccta tcgttacttt
    49921 ctttacctat cttgttactc ataaacatat tgaaggcgct tcaggctggt tctggttcgg
    49981 tgtgattgct gctgttattt caggcggtac tgcgattatc actgccttgg gcacaaaaga
    50041 agtcgatact gcgatccgtc agcaaaatga aaaagctggg atcaaagatg tgttcaaagc
    50101 agttgctcaa aatgatcagt tgatgtggct tgcccttgct tatatcgctt atgcgattgc
    50161 caatgcagca acaactgccg tattattcta cttcttcaaa tatgtacttg gccaaccaac
    50221 tgaattttgg atcgttggtc tagttgctgc cttacttggt ttagtagctg ttccactatt
    50281 cccaatgctt acacgcgtca tctctcgtcg ctacgtttac cttggaggga tcgcaatgat
    50341 gatttcttct tactttttct ttacgatcgc aggttcaagt ttgccaatcg ttatcatcgg
    50401 gctagtattg ttctacttcc ctcaacaatt gattttctta tccgctttga tgacgatcac
    50461 agactctgtt gaatatggtc aatggaaaaa tggtatccgt aacgaagctg ttactctatc
    50521 aattcgccct ttactagata aaatcgctgg tgctttgtct aacgggatcg tagggtttgt
    50581 cgctatcgct tctgggatga ctggtaatgc tactgctgct gatatcactg ctcatggcgt
    50641 aacgactttc aaagcttatg cattctatgc tcctgctgct ttgatgattt tctccgctat
    50701 tatttttgct tggaagatta cattgacaga gaaaaaacat gcacaaatcg ttgagaaatt
    50761 ggaaaacaaa ttggctccag ctgatacaat ggaagattaa cttcacgatg cagtagttcc
    50821 ttcccattaa gaaaggatct tgaaaatgaa gattacagaa aaagattttg gccaaggtta
    50881 tcacttgata accttggaga acaaaaacaa gctagcactt tctatctctg atttaggtgc
    50941 tagaatcgtc agtttaaaat caaatgatcg agaattagtt ttgggatttg acaccgctga
    51001 agaatatatc gaaaaagatc cgtatatcgg tgcttctatc ggtcgcaccg cagggagaat
    51061 cgaaaatggc cgcttttctc tcaatggaaa aacttaccaa ttagccacag atccaaaaac
    51121 tggacataat ctgcatggtg gtgctcctgg atttgaatta aaaaaatggt cttatgtcat
    51181 tttgaatggc gaaaatgaag cttctgtcat ttttactact acaagtcccg acggagaaca
    51241 tggttttcca ggtacaatgg atgtggaaat ccgctatact ttgacaaaag ataatatctg
    51301 gcgagtgacc tctcgcggca ccagtgacca agatacttta ttcaatccga caaatcatgt
    51361 ttacttcaac ttgacgggag atgcttctca gtcaatcgat caacatgagc tttggctcaa
    51421 tagcgaagct tatgctcctt tgcgaacaga tagtattcct ataggggtaa aagaaaatgc
    51481 tgcaggaagt gctttcgatt ttcaaatacc taaaaaatta gcgtccgttt ttgctagtga
    51541 tttggatcaa aaaaatcttg ttgacggaat cgatcatcca tttttcttaa aagaaaccgg
    51601 cttgggtaaa gaagccgctc gcttgactag ccccgataaa agaatccaag tagatattgc
    51661 tacagatgct tctagtgtgg ttattttcac tgcaaatttc ggcacagaaa caccagaaat
    51721 gcgcaaccga aaactggcac atcacggtgg catcacattt gagactcaaa cagctccagg
    51781 tgcagagcgc ttctcagcat ttggttcgat tcatttaaaa gccggatcag tatttgaaac
    51841 agtaacagaa ttcaaaatca aaacaagaaa ggaataactc atcatggaaa tcaaaagaag
    51901 cttaaacaca aaatttgccg aaacattcgg acctaaaaaa actacacaat acttctcacc
    51961 aggacgcatc aatttgattg gtgaacacac agattacaat ggaggccatg tcttccccgc
    52021 ttccatcact tatggaacgc aaggtgtagc agcacctcga gacgataata aaatattagt
    52081 ttattccaca aactttgacg atatcggtgt cattagtttt actttagatg agctagaata
    52141 tgataaaaaa gcaaactggg caaattacgt aaaaggaatg gtccttaaat taaaagaagc
    52201 tggacactca atcgatcatg ggtttgagtt attagtggaa ggaacaatcc ctaatggtgc
    52261 aggactttcc agtagcgctt cattagaatt attagtaggt gttgtattag aagatctgtt
    52321 tgatttaaaa acagatcgct tggaactagt ccaaacaggt aaaaaagtag aaaatgaatt
    52381 catcggtgtc aattcaggta tcatggatca gtttgccatc ggcttcggtg agctagatca
    52441 tgcaatctta ttagatacaa atacgctgaa atacgaaatg gttccagtga aactagatgg
    52501 ttatgcaatc gtcatcatga atacgaataa gcggcgggaa ttggcagatt caaaatacaa
    52561 tgaacgccgt agcgaatgcg aagaagcctt ggcacgtttg caaaaaactt tgcctatctc
    52621 ttctttagga gacttagatg aagaagaatt tgaatcaact attgaccaaa tcggcgatga
    52681 tacattgata cgcagagctc gccatgccgt ttctgaaaat cagcggactt tgaaggcaaa
    52741 agctgaatta gaagctggga acttagaagc atttggtcaa ctgttaaatg attctcacca
    52801 ctctcttcgc tatgattatg aagtaactgg gatcgaatta gatacactag tagatgctgc
    52861 tcaaaaacaa gaaggtgtct taggtgcacg aatgacagga gctggatttg gtggctgtgc
    52921 gattgctctt gtcaaagaag aaaatatacc tgccttcaaa aacaatgtct atgatgaata
    52981 catgaaagtt gtcggctatg ctccggaatt ttatgttgca cacattggaa atgggacaac
    53041 aaagatcgat ccagaagctg ttcatgtttg ataaaaaaat caacgatctc agctaaaaac
    53101 aaatagataa aaataacctc tcagcaattt ccaaatgtga gccttttgct cgtatttgaa
    53161 aattgctgtt ttttattgac acggaaagag aacgtgtgtt ctaattgtgt tgaagaggtg
    53221 attgttatgt gggcgaatca aaatccggta tttgtttatg aaaaagaacc ttcaagagat
    53281 attttatgta ttgactgcaa atctttttac gcctccgtag agtgcgttga aaaggatctt
    53341 gatcccctta caactaaatt agtagtcatg tcctatccgt ccgattcgac agaaacacgg
    53401 ggtagcggat taatcttggc ttccagtcct gcagcaaaaa aagcttacgg catcaccaat
    53461 atcagccgtg ccagagatct tcccttcccc tatccaagtg atttatatat tgttgcccca
    53521 aggatggcct attatatgga aaagaacaga gaaatcaatt ctatctataa aaaatatgca
    53581 gacgaagcga accatcatgt ctattccgtc gatgaaagct tccttgatgt aacaaatagc
    53641 atggcactct ttggtaaaac aaatgcgtac gacatggcta aactgatcca taaagatgtg
    53701 tttgagcaga ccggaatcta cacgactata ggaattggcg ataacccgct acttgctaaa
    53761 ctagccttag acaatggctc aaaagacgga aagcaaatgt ttgctgaatg gcgttatgaa
    53821 gatgtaccag aaaagttatg gaaaatctca gatatcactg acttttgggg aatcggcaaa
    53881 cgaacagcaa aacgtctgaa acaaatgggt atacattctg tttatgattt agctcacgcg
    53941 gattattatc acttgaaaag caagctaggg gtcatcggta ctcagcttta tgcccatgct
    54001 tggggcatcg atcgaagttt tttgggacaa gaatatacac caaaatcaaa aagtatcgga
    54061 aatagtcaaa tcctagatag agattataca agacgagaag aaatcgagat cgttatcaaa
    54121 gaaatggccg accaagtcgg cactcgcttg agacgatctg cagcaaaaac acaagtagtc
    54181 agcttatggg taggatactc tttaggctat cttgatccaa atggcaagac cgggtttcac
    54241 cagcaaatga aaatcgaagc gacaaatgct agcagagatc ttgcagatgc tttattgacc
    54301 attttccacc agcattacca ctaccaggat attcggcgtg taggcgtgaa ctgttccgac
    54361 ctcgtatacg cgacaggttt acagcttaac ctttttgaag atcctgaaaa gcagataaat
    54421 gaagcgaaag ttgattttgt tgtcgatacg attagaaaaa aatacggctt tcaatcaatc
    54481 atccatgcgc atagtctctt gcaaggagga cgcgccatcg cacgtagttc tctcgttggc
    54541 gggcatgcag gtggtatgag tggattagaa ggtggaaaag atgagacgga cagcaaaaaa
    54601 attcattgac tataacgagt atcgagatcg gccatttgga ttaaaatggg ggactgcttt
    54661 tgcgatggaa gaactagtca acggtatcaa aaaaatgaag aagaagcacg caaacagcct
    54721 cttcctaaac aacaaatgac attgcaagaa atcgatagta tactgacaga agccttttta
    54781 tctcgtaaag cagtaatcat tcagctcaat ttaaaagatg aatttggaag actcttggat
    54841 gaggtcgaag ggcacttcat tggtgaagcc tatgaagatt actttatgat tgaccatcag
    54901 ccgatattgt gggaagacgt acggtatatt gagttaaagg aaacaaaaaa atggtttgtg
    54961 acatgagagg ttgtgacaga agtgatcaac ttcaaaaaat aagaaaggat ttttcgaaaa
    55021 ttgcttctta aatttttgtg gaattttggc ttatttttcg aagaagcaac ttctggaaca
    55081 acgtatatta gatatcagag gttgtgaaaa agtcgcttag ttttgagtaa caagccaagg
    55141 cgctgatttc acaacctctg tctatctatc catctattcg gtaagattca tgcatgacgt
    55201 cttctttatt tagtatgtag agcccttttt cttctgacca ttcaacataa atcagctgtt
    55261 ttacatctgc atagccttct ttcttcaagt tctccataag ccatgcttca gtcaattgat
    55321 tggactgaag cactttatgc tgaatttgcc cttcatctac aagcaggatc gataaagctt
    55381 tgtcttcatc tgattttttc aatacactca tttgaccggc attctctagg ataacatgtt
    55441 tggcattttc aagaaaaaag cattcttgct gtcttaagat tgctcgcaac tgctccatct
    55501 cgatatggtt atttgtcaac tctgtcatgt tgatgacgcc gtctttgatt agaacagatg
    55561 gctcgccttt gacccaacga ttgacctttt catttttttg gacgatacgt tcgataccat
    55621 aaatcattac tgcccaaagc gcaatggcaa ataggacatg ccctacatgg acattgtcat
    55681 cataggttga ttcttctaag atcccaccta aaacaagcgt atagactaaa tcaaacggtg
    55741 tgatttctga catggatttc ttcccaagca aacgtaccac taataataaa ccgatcaacc
    55801 cgaacaccag tttcattgta attgaaaaaa tcattttttc tccccctaac tatctgttaa
    55861 ttaaattata tatgctttct cacgaacacg catggaatat acaaggaaga aatgtatcct
    55921 tttactaaaa aaaggactgt gacactgtag tgacccccaa aagttagact tttttggagt
    55981 ggagaaatcc actccttttt tttatgctct aaaatgatat ttttacacaa ccgttacggt
    56041 tgttttacga aattggacg
//
LOCUS       DAZBTF010000011        53359 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000011 DAZBTF010000000
VERSION     DAZBTF010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.011"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            425..1153
                     /locus_tag="ACHGNS_000726"
     CDS             425..1153
                     /locus_tag="ACHGNS_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN6936697.1"
                     /translation="MESSSKGKSLYYQLVDVLKERIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELETQGYIYRRHGKGTYVSDLSDKSSDLAGAYSFTEEMRELGRVPNTV
                     ILEFKQMEANKKVAKNLNINLGETVFKLKRLRLADDEPLMVERSYLPAKLFIGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDADPLRIADGAPVLYLSRTTYNVKN
                     EIIEYTDSVARADQFHYKIRHVRK"
     gene            1171..2364
                     /locus_tag="ACHGNS_000727"
     CDS             1171..2364
                     /locus_tag="ACHGNS_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFN6936698.1"
                     /translation="MFELSKDKLKELGAEITTREIKQQPQLWKETAEILAQNQEKLAL
                     FFDQIKKAANGKRVRVIFTGAGTSQYVGDTAVPYLNEYGDNDHFIFHSFGTTDIVSSP
                     KEFLFPDEPTLLVSFARSGNSPESVATVKIANEQIKTLFHLTITCAKEGQLAKNAATD
                     ERNYLLLMPEKSNDAGFAMTGSFTCMLLSALWVFDQTAVEKKESYLQQLIRTGEDLIS
                     RDQEIQKLLPKSFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVIGFVNNQPYTRNYDLDILEEIKDDQITTSVIAIAQPGNIDFTGTSLLLE
                     ASISLPDAYLALPMIMTAQIIALDTSIKVANKPDTPSPTGTVNRVVKGVIIHEYVNKG
                     ETKDE"
     gene            2357..3352
                     /gene="lacD"
                     /locus_tag="ACHGNS_000728"
     CDS             2357..3352
                     /gene="lacD"
                     /locus_tag="ACHGNS_000728"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFN6936699.1"
                     /translation="MNEEKIAAMKSLSTKEGIISALAIDQRGALKKMIKALGKEPTAQ
                     QITEFKELVSAELTPYASAILLDPEYGLPAAKARHQEAGLLLAYEKTGYDATTPGRLP
                     DLLADWSTLRLKEQTADAIKFLLYYDVDEDPEINHLKHVFIERLGSECAGEKRPFYLE
                     LVSYDAKTNDSTSLEYAKIKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVEGYSDG
                     EIAYSKKEAAAFFKEQSDATPLPFIFLSAGVSTALFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGREGAIEWLRTQGRQNIESLNKVINENATPWFDKVKEDSVTAK"
     gene            3349..4290
                     /locus_tag="ACHGNS_000729"
     CDS             3349..4290
                     /locus_tag="ACHGNS_000729"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HFN6936700.1"
                     /translation="MITTVTMNPSIDIAYTLDELRIDQVNRVKTVNKTAGGKGLNVAR
                     VLHLLADDVLATGLVGGQHGAYIKEQLDQSGITHQFLPIKGETRDSIAILHGVNQTEL
                     LESGPVVTEEEQHKFTEHFARLMETTDIFTISGSLPQGFSKSYYAELLALTNKKGTRV
                     LLDTSGQTLLEVLEGKEKPFLIKPNLEELGELVHQTISSATSLDEIQSLLDHPLFEGI
                     NWIVLSLGAKGAIAKVGRHFYQVTIPKIAVVNPVGSGDSTIAGLAHALSKSYTIEETL
                     AFAMACGMANAQQEKTGWIDPVKVDYFRGEINVRKIR"
     gene            4336..6102
                     /locus_tag="ACHGNS_000730"
     CDS             4336..6102
                     /locus_tag="ACHGNS_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase family protein"
                     /protein_id="HFN6936701.1"
                     /translation="MTRFEIKEEFLLDGQPFKILSGAIHYFRVHPTNWERSLHNLKAL
                     GFNTVETYVPWNLHEKEEGTYDFSKILDLSHFLELAQSLGLYAIVRPAPYICAEWEFG
                     GLPAWLLTKECRIRSADPRFMKYVKRYYQTLLPKIVPHLVTHGGNVLMVQVENEYGSY
                     GEEKAYLRQVKKYLEEGGIDVPLFTSDGPWQATLRAGILIDDDVFTTGNFGSKAKENF
                     ADMAQFFEAHGKKWPLMCMEFWDGWFNRWKEPIIKRDPEELADAVKEALQIGSINLYM
                     FHGGTNFGFMNGCSARGAIDLPQVTSYDYDAPLDEQGNPTEKYFALKRMMAENFPEMQ
                     QMEPWIKETIVKKGINLKEKVSFFATLDTISKPIHSTYPQTMEELGQNTGYLLYRTTI
                     KKDAVNEKLRIIDGRDRVHVFLDEEKQAIQYQTEIGETIPLTLEKEDHQIDLLFENMG
                     RVNYGHKLLADTQRKGIRTGVMSDLHFITEWTQYCLPLETTEHIDFSKKWEAGQPAFY
                     RFEAELSEIGDTYLDLSEFGKGIVWVNGTNIGRFWEVGPILSLFVPEGLFRKGQNEIV
                     IFETEGRFSEVIDFVKEPIEKS"
     gene            6119..6598
                     /locus_tag="ACHGNS_000731"
     CDS             6119..6598
                     /locus_tag="ACHGNS_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFN6936702.1"
                     /translation="MAIIGVRIDGRLIHGQVANLWTTKLNIGRIMVVDDEVATNAIEK
                     SGLKLATPAGVKLSILPIEKAAANILAGKYDSQRVLIVAKKPDRILALVEKGVPIKEL
                     NVGNMSQTDETRSITKSINVVDADVEVFKKLDQLGVHLIAQMVPNDKAEDFMSLLIK"
     gene            6611..7501
                     /locus_tag="ACHGNS_000732"
     CDS             6611..7501
                     /locus_tag="ACHGNS_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFN6936703.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLKAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDQHVETMNYKAIERNFLMGALPWALSRMVPVFLAL
                     AFGGGLVQNVVAVLNGDLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHISYLILGFT
                     ITALLATVFSNVQLLGTSVASVVKDFPGTFNSLPMLAVALIGLALATISYKNDQKNDQ
                     HPVVSQQSQSDEGEIEDDEI"
     gene            7488..8297
                     /locus_tag="ACHGNS_000733"
     CDS             7488..8297
                     /locus_tag="ACHGNS_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN6936704.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDDTPELKEMMKTHTQFFNTSNFFNTIITGIDLAIEEKEGVSSKETVSGMKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPVGILIWILAQVAVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKLLPAGIVGGVYWMLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            8313..8717
                     /locus_tag="ACHGNS_000734"
     CDS             8313..8717
                     /locus_tag="ACHGNS_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN6936705.1"
                     /translation="MSRQLVLISHGKFCEELKNSTEMIMGPQENIYTIPLLPEDGADT
                     YKERFLSVTAPLKDYVVLCDLLGGTPANTVSKLIMEGETIELYAGMNLPMVIEFINSQ
                     MIGAEPDFIGSGRESIVSVNQVIAGMGDDEEE"
     gene            complement(8898..9836)
                     /gene="yidC"
                     /locus_tag="ACHGNS_000735"
     CDS             complement(8898..9836)
                     /gene="yidC"
                     /locus_tag="ACHGNS_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFN6936706.1"
                     /translation="MNKWKNWLLGSGLVTIMLFLSGCVRMDSNGNPDTSGIIYRILVH
                     PMGQAITYLVENFNWSYGWAVILMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     ATQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESTFYGIDLGNPSLVLVAIAGIAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGIFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTPAEPAKQTKKQVQAPKNNAKNGRNAGKQQKRK"
     gene            complement(10170..11252)
                     /locus_tag="ACHGNS_000736"
     CDS             complement(10170..11252)
                     /locus_tag="ACHGNS_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFN6936707.1"
                     /translation="MTKNLIVRGAPQEYEIKENAWDSLPEHLYKRHIDNVLILHGKTS
                     WKAVKDVFPDLSITKIFEYYGGECTDPLADSFVSLCKKKDFNGIIAVGGGKVADLGKL
                     IAYKTNLPIIILPTLAATCAAYTPLSVVYNEEGVMLRYDMFPKANDLVLIDPKIILHS
                     PREMMIAGIGDTLAKWYESHAIISQLTIKPIEVQVAEFAALRCREILLEKSAEAMEAM
                     DQQKINQAFIDVVETNILLAGMVGGFGDDYGRTAGAHSIHDAMTVLPDSHQQLHGNKV
                     AYGILVQLMIENKLAEIEHLLPFYHHLNLPTSLADMGLHLTESDYQAVAERACLPDEM
                     IHFMKETITPALVVQKMKELEQVTQG"
     gene            11394..12845
                     /locus_tag="ACHGNS_000737"
     CDS             11394..12845
                     /locus_tag="ACHGNS_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFN6936708.1"
                     /translation="MKKGINILLVALMFLLVFLPVKVQGAEKDEIYERIKQSGELVVG
                     LSADYAPYEFHANVDGKDKIVGFDVSIAEKIAKDMGVKLKVEELGFDALLGALKTGKI
                     DLVISGMAVTEERLQEVDFSDPYFVVQQKVLVRKEDKNKFKTTADFSGLAVGAQKQTT
                     QEELVKNELTGAELTSLQKVPDLINNLLNEKVEGVVLEQPVAEAYAQQNKKVALTPVK
                     FAQGEKTTAIAMSKNTPELKNHVNASIKTINDDGLLKDYQKQANQLMFQDDQSFLQKY
                     GPYYLIGTKYTILLAFVGVLFGMVFGSLLALMKLGKNKFLKALAIIYIEYVRGTPLLV
                     QIFIVYFGTGVLGLDLSKLVAGCIAVSLNSAAYVAEIVRAGINAVNKGQMEAARSLGM
                     DQKEAMRFIIFPQAIKNILPALGNEFVTIIKESSVVSVIGVSELIFQTGVVQGASFKP
                     FLPYVLVSLIYFVLTFSISRILALVERRMGLNA"
     gene            12838..13560
                     /locus_tag="ACHGNS_000738"
     CDS             12838..13560
                     /locus_tag="ACHGNS_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFN6936709.1"
                     /translation="MLNVKKLSKSFGENEVLKSIDLSVKEGEVVVIIGPSGSGKSTIL
                     RCMNLLEEPTSGEIFFKGQNITAPDSDINQIRQKMGMVFQSFNLFPHMNVLENLTITP
                     VKIKKEDPKKAKEHALSLLNQVGLKEKVDSYPTSLSGGQQQRVAIARALAMNPDVMLF
                     DEPTSALDPEMVGEVLAVMQDLAKKGMTMVVVTHEMGFAKEVADRVIFMADGIIQEEG
                     TPKEIFENPQNSRTQDFLNKVL"
     gene            13580..14731
                     /locus_tag="ACHGNS_000739"
     CDS             13580..14731
                     /locus_tag="ACHGNS_000739"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFN6936710.1"
                     /translation="MDKSKIARRFQQPAENLLMNIAILAKEKEDIIDLSIGDPDLITD
                     PTIIEAAYTDVKNGATNYTAPDGSKEFLSTVVDFYQKKYGLSVECNQVRATVGAMHGM
                     YLVLQALLDPEDEVIIHEPYFSPYREQVILSGGKPVFVPTYEEKGFQLDVEDLKAAIT
                     DRTKVIVINSPNNPTGAVFSESTLKKIAEVAIENELYIISDEIYEDFAFKEKFVPMAR
                     FAPDNTITVSGVSKGFAMTGWRLGYMIAPAYINQIAGLINENITYSAPTASQRGAIYA
                     LKHYEQLVPPVVEVFKERLAYIEKRVAEIPYLSLSSVSGSMYAFINISKSGLDSVTFV
                     EEVLEKSGVLFVPGKAFGETIGDNYIRLAATQPLEILGEAFDRLAKLNF"
     gene            complement(15079..15354)
                     /locus_tag="ACHGNS_000740"
     CDS             complement(15079..15354)
                     /locus_tag="ACHGNS_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFN6936711.1"
                     /translation="MNKIKMNVQGRVQSVGFRYMTKIVADQLGITGTVKNEDDGSVTI
                     EAAGDDEAIKKFVEEVKRSPSPSGRVQYVDLQENPMMEDRKKFVVIG"
     gene            15462..16226
                     /locus_tag="ACHGNS_000741"
     CDS             15462..16226
                     /locus_tag="ACHGNS_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFN6936712.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKGEYLLEGFHLIEEAVAS
                     NAKVNELFITQRGLTEWASWLEENQALSVTLVSEEVMATVSDLPTPQGIMAVVEAEKR
                     EIPNFEGGWLLLDNVQDPGNVGTMIRTADAFGLSCVVLGKGTADVYSTKVLRSMQGSN
                     YHLPIIQEDLTKLLPILKEQGFITYGTELNEEAVSLNDIQTTENYAIVMGNEGQGVRP
                     EVLALTDKNVYIPIQGNAESLNVGVAAGIMLYHFSV"
     gene            16350..16853
                     /locus_tag="ACHGNS_000742"
     CDS             16350..16853
                     /locus_tag="ACHGNS_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFN6936713.1"
                     /translation="MTEKWREDEEYMSYINDLLETDAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYKLAKKWGGDARATARAGLLHDLFYYDWRTTKFDEGTHAYVHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMTASMKQKWQRY
                     FGKEQSI"
     gene            17118..17765
                     /locus_tag="ACHGNS_000743"
     CDS             17118..17765
                     /locus_tag="ACHGNS_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFN6936714.1"
                     /translation="MEKTIKPYQYYFIKREVEQLLSAYSSVNDPKTVQTVQALAAEKI
                     HEVLGHPNTAVNEFMEKVLDVTLKKEKAERLLEELKVIVLPFIQPTKPQIDKLFRKVK
                     KLKQPDWNQLDLREHTYIGWNDPGSQKKFMIYYEEEKLKGISGTLSPTINKGICSICQ
                     RTSNVSLFLSTTKTGSDGTYTKKGNYICHDSDQCNQQLVQLAPFYEFTERVKRER"
     regulatory      17808..17875
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            17926..19194
                     /locus_tag="ACHGNS_000744"
     CDS             17926..19194
                     /locus_tag="ACHGNS_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFN6936715.1"
                     /translation="MENRLPDPLKVIIYMIKWLLITAILGIFMGSLSAFFLTSLTDVT
                     AIRLEHPWLLFLLPISGGLFAYLYTTYGGNSSRGNNLVIDQGNGGNEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALAENFARWLKLNKIEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEALFPSFFAALFANFVTESFNVKHTHYQMGAIPAWDPS
                     LFFKLLLAGIAFGIIGWLFSRSIVWLKAWYAKRIPHPVLRNFIGGAVVVLVVVILQTQ
                     RYLGLSLPLLQDAFNGSAHPYDFLGKLGFTVLSLGAGYQGGEVTPLFEIGATLGATLA
                     PILHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGGQAASYFFMICLISFMCSGN
                     SGIYSAQKVEVKKGILFLPILGRISRQKEK"
     gene            complement(19371..20573)
                     /locus_tag="ACHGNS_000745"
     CDS             complement(19371..20573)
                     /locus_tag="ACHGNS_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFN6936716.1"
                     /translation="MNLTRDLIFLVIYILGMSGILYWRKRQSDPALFFYCGTWTFMMM
                     VTYFVVLEVFYHTFYFLIPLLFFSIFAFFYFKEKRRLINGVLFNTFLIVFGGYLGWLL
                     IETQNIFIGGLLALIGIPILILLAIGIYILIIFLFWNGVVVMKKESRSLANLLTLLLG
                     VALTIFLILDYTLVHWLPQWANALFAVIPFIFAYLLIVFFNFMTVSILYQFNRPRYNQ
                     DYIVVLGAGLIDGERVSPLLAKRINKAIAFYWAQSKATLNPPVLLMSGGKGTDEKVAE
                     AIAMKQYALEKGIPERDILVETNSTTTLENMLFSKEIMDQQMNGPYRAIFTSNNYHIF
                     RAGLYARQAKLNANGIGAKTALYYLPNAILREFIAILVLHKKRHLIICGLMIGFAVFS
                     ALILKFAQ"
     gene            21100..21825
                     /locus_tag="ACHGNS_000746"
     CDS             21100..21825
                     /locus_tag="ACHGNS_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTC domain-containing protein"
                     /protein_id="HFN6936717.1"
                     /translation="MNKKRFKRIERKFALTYDQAKYLKTLFPKQVVRDKYSDHFISSL
                     YFDTNDHHFLKEITTEKSYREKFRIRKYGDPQDISPIYLEIKRKFQGMTDKRRIKLAH
                     HELDDMLENIELAETTFSNLEEEYIFNEIQWLFRRLALEPKVYIYYLREAFVNRDSED
                     LRITFDQNIHYRLMNLTTWEHCTEDNLVAPEIDVLMEVKTTGEIPEWLQVGLDRMGKK
                     EIKFSKYKQVYKRYIQEDPLYVQ"
     gene            21815..22489
                     /locus_tag="ACHGNS_000747"
     CDS             21815..22489
                     /locus_tag="ACHGNS_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HFN6936718.1"
                     /translation="MFNSVLKNEPSQLTLDDFFLCMVVSILLGILIAYTHMYQNNCTK
                     NFMFALIGLPTLVHTVILLINGNLGTGIAVLGAFNLVRFRSAQGNSRDIFSIFWATSI
                     GLATGTGYIVYALFVSSIVAAIQVIIFTLPIKNKHTVFNTEKTIKLKVSKSLFLSSII
                     EEKIAEYTENCNLKKVKINKETCSLHYIVKVKTSMLEKELVRTIMDHPEVISITVGMD
                     KSNKLL"
     gene            complement(22777..23061)
                     /locus_tag="ACHGNS_000748"
     CDS             complement(22777..23061)
                     /locus_tag="ACHGNS_000748"
                     /inference="COORDINATES: protein motif:HMM:NF024076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HFN6936719.1"
                     /translation="MRQKCFLLLSHSQKVKPIFWLSIWDGFFKVSFHFPEKTREKLVT
                     LSLSEGTKEKIKTLQANGNKRKHLSVIFDVDHLEQLADIYQLAEFKKQNG"
     gene            complement(23355..24662)
                     /locus_tag="ACHGNS_000749"
     CDS             complement(23355..24662)
                     /locus_tag="ACHGNS_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFN6936720.1"
                     /translation="MFKKNTRFIVLLASLILCITSFLPSATVQADDAFKINAKAAFAV
                     DAESGKILYDQDGEKTMGIASITKIIGLYIVLEQIKEGKLSWEDKVAISDYAEKLSVV
                     PDLSNVPLHKENDYTVKELVDSAFIQSANASMVALAEKVAGSESKFLELMKKQLKDWG
                     IKDATIVNASGLNNSYLGENRPENTGETDENQLSAKDVAIVARHLILDYPEVLKITST
                     TNQTFGENTQSPVEMVNWNWMLPGFVNYHEGVDGLKTGTTDFAGACFVGTINKDGRRI
                     ITVVLNADNHEENPSARFNETSRLMDYCYDNWSEKELGKANASIPSLKDIKVKDGKET
                     SVNVALKSPVKVWVENGMDTGKLTITPTIDKKKVTDDQVTAPINKGTTVGSATIQLAD
                     DKLGYLEANQEPSTDIITTSTVEKANIFVIGWRHVTDFFSNLF"
     gene            25151..26257
                     /locus_tag="ACHGNS_000750"
     CDS             25151..26257
                     /locus_tag="ACHGNS_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936721.1"
                     /translation="MAKKEKELLSQLTSEGTKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKITSLLDDPSFWRSATTSVAVIVSPEQVFIHRLGI
                     PVDDQYYVSDLPYLLAIIKNGHFNYSYYLLGLNRDSMKLYEVTNKQVKEVALPEDAPR
                     DVVTALGSELTGGGLNYASNGSEGTTFHGVNPKDEEVEIDWVNYYQAVDDYLKDTFEN
                     PEKWPIFLFALPENQTMFKKVAKNPYYRSDVAISASPAQLSLKEIEAGAKKLSQELAV
                     KEASDYNKLLDKKFIDQLVDIIPAAKEGKISHLFIATSNLADGFGEDPETEYDRRQVL
                     NELADNVITNGGQVFILEQKDAPDEKSLVAILRY"
     gene            26848..27048
                     /locus_tag="ACHGNS_000751"
     CDS             26848..27048
                     /locus_tag="ACHGNS_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN6936722.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            27195..29984
                     /locus_tag="ACHGNS_000752"
     CDS             27195..29984
                     /locus_tag="ACHGNS_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFN6936723.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIEAGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSSHTGMDTSRFIYHVLEEMKENPEPLDLSLEIIDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIMEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPGQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHAFFADVAHRGTGFLARNQSFYKQDFVRFLQKKIKQSNFLIVNHAFLVQETQRKEP
                     LLPASPYLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICVAEGLMNLVSSKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAMDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLLDFQAAMLPSTVWYPRYDHILYIGGTLKIGP
                     NRQYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLEAALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAIDEI
                     YQDIHEFLKKNEEGS"
     gene            30031..30558
                     /locus_tag="ACHGNS_000753"
     CDS             30031..30558
                     /locus_tag="ACHGNS_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFN6936724.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            30579..31769
                     /locus_tag="ACHGNS_000754"
     CDS             30579..31769
                     /locus_tag="ACHGNS_000754"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFN6936725.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            31809..33107
                     /gene="asnS"
                     /locus_tag="ACHGNS_000755"
     CDS             31809..33107
                     /gene="asnS"
                     /locus_tag="ACHGNS_000755"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFN6936726.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            34041..34715
                     /locus_tag="ACHGNS_000756"
     CDS             34041..34715
                     /locus_tag="ACHGNS_000756"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFN6936727.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWREDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQKAGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            34712..35053
                     /locus_tag="ACHGNS_000757"
     CDS             34712..35053
                     /locus_tag="ACHGNS_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936728.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVGQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            35083..35442
                     /locus_tag="ACHGNS_000758"
     CDS             35083..35442
                     /locus_tag="ACHGNS_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFN6936729.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     AFLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            35966..38047
                     /locus_tag="ACHGNS_000759"
     CDS             35966..38047
                     /locus_tag="ACHGNS_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFN6936730.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNELIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            38358..39824
                     /locus_tag="ACHGNS_000760"
     CDS             38358..39824
                     /locus_tag="ACHGNS_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFN6936731.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            40109..40607
                     /locus_tag="ACHGNS_000761"
                     /pseudo
     CDS             40109..40607
                     /locus_tag="ACHGNS_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
     gene            40681..41121
                     /locus_tag="ACHGNS_000762"
     CDS             40681..41121
                     /locus_tag="ACHGNS_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFN6936732.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQKGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            41134..41343
                     /gene="copZ"
                     /locus_tag="ACHGNS_000763"
     CDS             41134..41343
                     /gene="copZ"
                     /locus_tag="ACHGNS_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFN6936733.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            41343..43529
                     /locus_tag="ACHGNS_000764"
     CDS             41343..43529
                     /locus_tag="ACHGNS_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFN6936734.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            43549..45720
                     /gene="copB"
                     /locus_tag="ACHGNS_000765"
     CDS             43549..45720
                     /gene="copB"
                     /locus_tag="ACHGNS_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFN6936735.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(45832..48003)
                     /locus_tag="ACHGNS_000766"
                     /pseudo
     CDS             complement(45832..48003)
                     /locus_tag="ACHGNS_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(47993..48745)
                     /locus_tag="ACHGNS_000767"
     CDS             complement(47993..48745)
                     /locus_tag="ACHGNS_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFN6936736.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTAYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            complement(48742..49065)
                     /locus_tag="ACHGNS_000768"
     CDS             complement(48742..49065)
                     /locus_tag="ACHGNS_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936737.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            complement(49079..49954)
                     /locus_tag="ACHGNS_000769"
     CDS             complement(49079..49954)
                     /locus_tag="ACHGNS_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN6936738.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            50281..51570
                     /locus_tag="ACHGNS_000770"
     CDS             50281..51570
                     /locus_tag="ACHGNS_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936739.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFYFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLIKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQALAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            51644..52213
                     /locus_tag="ACHGNS_000771"
     CDS             51644..52213
                     /locus_tag="ACHGNS_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFN6936740.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYKTLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(52389..52565)
                     /locus_tag="ACHGNS_000772"
                     /pseudo
     CDS             complement(52389..52565)
                     /locus_tag="ACHGNS_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            52644..53342
                     /locus_tag="ACHGNS_000773"
                     /pseudo
     CDS             52644..53342
                     /locus_tag="ACHGNS_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
BASE COUNT        17436 a         9325 c        11247 g        15351 t
ORIGIN      
        1 agaattatca taaaaaattt ttgttatttt taaaaatgta caacaaaatt tcgactatca
       61 gaccggcatg agagaaaagt tatctaaaaa ataaaaaatc ctactttatt tagagaaagc
      121 catttttatt tacatagtag tctattaggc ccatttgctt gtcgatagta gaaatagcca
      181 cagatgtata aagaatattt tttctttact tctctagacc atttagctga atctttgcca
      241 agattcagct tttttttgtt ttttatttgt caactggtat ttttgaggta tcttttgata
      301 ccagttctaa attaccttga aaccttgtta taatagcttt tttacgccaa aaaccctttg
      361 acaaatgagg aatatgtggg tattcttgtg ttgtggttat aaccagtaaa gaagaggtat
      421 ttttatggag agctcaagta aaggaaaatc attatattat caactagttg acgtattgaa
      481 agaacgtatc gaaacacaga tgacacctca tgacaaattg ttatctgaaa gagaactttg
      541 tcagcattat ggagtgagtc ggacaacggt tcgccttgct ttgcaagaat tagagacgca
      601 agggtatatt tatcgccgac acggaaaagg aacatatgtg tctgatctat cagataagtc
      661 gtctgatctg gcgggagcgt atagttttac agaagagatg agggaattgg gacgggtgcc
      721 taatacagtc atcttggaat tcaagcaaat ggaagcaaac aaaaaggttg ccaagaatct
      781 aaacatcaat ttaggcgaaa cagttttcaa attgaaaaga cttcgtttag cggatgacga
      841 accattgatg gtggaacgtt cttatttacc tgcaaaacta tttattggtc ttgatctgcc
      901 attacttgaa agtaagccat tgtatgatgt gttttctcag gattatgaac agcaagtcca
      961 attagcagat gaagaatttt atgcaagtat cgttcaaaag aaagatgctg atccgcttcg
     1021 tatcgctgat ggtgccccgg tattatatct aagccgaacg acctataatg tgaaaaatga
     1081 aattattgaa tatacggata gtgtagcgag agcagaccag tttcattata agatccgcca
     1141 cgtccgaaaa tgattgagag ggaaaacaac atgtttgaac tatcgaagga taaattaaaa
     1201 gagttgggtg cggaaataac tacgcgagaa atcaaacagc aaccgcaatt atggaaagaa
     1261 acagctgaga tattagcaca gaatcaagaa aaattagcat tgttttttga ccagataaaa
     1321 aaagcagcaa atggcaaaag agttcgagtg atttttactg gtgcaggcac atcacaatat
     1381 gtcggggata cagcagtacc ttatttaaat gaatacggtg ataacgatca ctttattttt
     1441 catagctttg gtacgacaga tatcgtgtct tcaccaaaag aatttctatt tccggatgaa
     1501 ccgacattac tggtgtcatt tgcacgtagt ggaaatagtc cagaaagtgt ggcaacagtc
     1561 aagattgcca atgaacaaat caagacattg tttcatttga cgattacttg tgctaaagaa
     1621 ggtcagttag caaagaatgc agcgacagat gaacgaaatt atcttttact gatgccagaa
     1681 aaatcaaatg atgcaggatt tgccatgaca ggaagtttta cgtgcatgtt gttgagtgcg
     1741 ttatgggtat tcgaccaaac tgccgttgag aaaaaagaaa gttatcttca acaattgatc
     1801 agaactggag aagatctaat ttcacgagat caagaaatcc aaaaattatt gcctaagtca
     1861 tttgaacgta tcgcctatct tggttcagga agtttggctg gtgcaacgag agaagcacaa
     1921 ttgaaaatat tggaattgac agccggtaaa gtggcaacga tctttgattc ttctatgggc
     1981 ttccgccatg gaccaaaatc gtttttaaat agcgaaacac ttgttattgg ttttgttaat
     2041 aaccaaccgt atacaagaaa ttatgactta gatattctag aagaaataaa agatgaccaa
     2101 attaccacga gtgtcatcgc gatcgcccaa ccaggaaaca tcgatttcac aggtacaagt
     2161 cttttgttag aagcttcgat cagcctacct gatgcgtatc ttgccttacc tatgatcatg
     2221 acagcccaaa tcattgcgct cgatacgtcg ataaaagtag cgaataaacc agatacacca
     2281 tcgcctacag gtacagtcaa tcgagtggtc aaaggtgtca ttatccatga atatgtgaac
     2341 aaaggggaaa cgaaggatga atgaagaaaa aattgcagct atgaagagtt tatcaacaaa
     2401 agaaggaatc atcagtgcat tggcaattga tcaacgcggt gctttgaaaa agatgatcaa
     2461 agcgttagga aaagaaccaa cggcgcaaca gatcactgaa ttcaaagaat tagtatcagc
     2521 agaattaacg ccttatgctt ctgctatttt actagatcca gagtatggat tgccagctgc
     2581 taaagccaga catcaggaag caggactact tttagcctat gaaaaaacgg gttatgatgc
     2641 gacaactcca ggacgtctgc ctgatttatt agcggattgg tctacgctta gattaaagga
     2701 acaaacagcc gatgcgatca agtttttgct ttattatgat gtagatgaag acccagagat
     2761 caatcatttg aaacatgtat ttatcgaacg tttaggcagt gaatgtgcag gtgaaaagcg
     2821 tccgttttat ctagaattgg tttcgtatga tgccaaaacc aatgacagca cttcattaga
     2881 atatgcgaag atcaaaccgc ataaagtgat cgagatgatg aaagaatttt ctgatcctaa
     2941 atataaagtg gatgtattga aagtagaagt gccagtcaat atgaactatg tagaaggtta
     3001 ttctgatggt gaaattgcgt atagtaaaaa agaagcagcg gcattcttca aggaacaaag
     3061 tgatgcaaca ccgttaccat ttatcttctt gagtgcaggg gtttcgactg cattgttcca
     3121 acaaacattg atctttgctc atgaatctgg ttcaacattc aacggtgtgt tatgcggacg
     3181 tgcgacttgg aaagatggag tcaaagcata tgttgaaaat ggtagagaag gtgcgattga
     3241 gtggttgaga acacaagggc gacaaaatat cgaatcgttg aataaagtga taaatgaaaa
     3301 cgccacccct tggtttgaca aagtaaaaga agattcggtg acagcaaaat gatcacgaca
     3361 gttaccatga atccttcaat tgacatcgct tacacgttgg atgaattacg gatcgaccaa
     3421 gtcaatcggg tcaaaacggt caacaaaacg gctggaggaa aaggcttgaa tgttgctcgt
     3481 gtccttcatt tattagcaga tgatgtttta gctactggat tagtcggcgg gcagcatgga
     3541 gcatatatca aagaacaact cgatcaatca gggataacgc atcaattttt acctatcaaa
     3601 ggggaaacga gagattcgat tgctatttta catggagtga atcaaacaga actattggaa
     3661 agcggtcctg ttgtgacgga agaagagcaa cataaattta ccgagcattt tgcacgacta
     3721 atggaaacaa ctgatatctt tacgatttcg ggtagtctgc ctcaaggatt ttctaaaagt
     3781 tattatgcag agttacttgc cctaacgaat aaaaaaggaa cacgagtatt gttagatact
     3841 tctggtcaaa ccttgcttga agtcttggaa ggcaaggaaa aaccattttt gatcaaacct
     3901 aatttggaag aattaggaga gttggtccac caaacaattt cttcggctac ttcgttagat
     3961 gaaatccaat cattattgga tcatccgctt tttgaaggaa tcaattggat cgttctttcg
     4021 ctaggcgcaa aaggagcaat cgctaaagta gggagacact tctatcaggt tacgattcct
     4081 aaaatcgcgg tggtgaatcc tgtggggtca ggagattcga ctattgcagg cttagcacat
     4141 gctctatcaa agagctatac gattgaagaa acacttgcgt ttgcgatggc ctgtgggatg
     4201 gcgaatgcac aacaagaaaa aacaggatgg atcgatcctg tgaaagtcga ttattttagg
     4261 ggggaaatca acgtcagaaa gattcgttga ttataaaatt agaaagcggt ttctaaaaac
     4321 gaggaggaaa tttgcttgac tagatttgaa ataaaagaag agtttttgtt agacggtcaa
     4381 cctttcaaaa ttttatctgg tgccatccat tatttccgag tgcatccgac gaattgggag
     4441 cggtcattgc acaatttgaa agcgcttgga tttaatactg tggaaaccta tgtgccttgg
     4501 aatctccatg aaaaagaaga aggaacctat gatttttcga aaatattgga tctttcccat
     4561 tttctagagt tagcgcaatc ccttggatta tacgcaatcg tacgtccagc cccttatatt
     4621 tgtgcagaat gggaatttgg tggcttgcct gcttggctat tgacaaagga gtgccgtatt
     4681 cgttcagcgg atcctcgctt catgaagtat gtcaaacggt attaccaaac gctactacct
     4741 aaaatcgttc ctcatctagt cactcatggt ggcaacgtct taatggtgca ggtagaaaat
     4801 gagtacggtt cgtatggtga agagaaagca tacctgcgcc aagtaaaaaa atacttggag
     4861 gaaggtggaa tcgatgtacc gctattcact tctgatggac cttggcaagc cacattaaga
     4921 gcaggcattt tgattgacga tgatgttttt acgacaggga attttggctc aaaagcaaaa
     4981 gaaaattttg cagatatggc ccagtttttt gaagcacatg gtaaaaaatg gccgttgatg
     5041 tgtatggaat tttgggatgg ctggtttaat cgttggaaag agccgatcat taaaagagat
     5101 ccggaagaat tagcggatgc agtcaaagaa gcattacaga tcggcagtat caatctctat
     5161 atgttccatg gcggaaccaa ttttgggttt atgaatggct gttcagccag aggcgcgatt
     5221 gatttgccgc aagtcacttc ctatgattac gatgcaccat tggatgagca gggaaatcca
     5281 acggaaaaat attttgctct gaaacgaatg atggcagaaa attttccaga aatgcagcaa
     5341 atggaaccat ggataaaaga aacaatcgtc aaaaaaggaa tcaacttgaa agaaaaagtc
     5401 agctttttcg caacactgga taccatcagc aagccgatcc actcgaccta tcctcaaaca
     5461 atggaagaat tgggtcagaa tactgggtac ctattgtatc gaacaacgat caaaaaagat
     5521 gcggtcaatg aaaaattacg gatcatcgac ggacgtgatc gggtccatgt ttttctcgat
     5581 gaagaaaagc aagcgattca ataccaaaca gagatcggtg agaccattcc attgacactt
     5641 gagaaagaag accatcaaat tgatctatta tttgaaaata tgggacgggt caactatgga
     5701 cacaagctgc tagctgatac gcaaagaaaa ggaatacgta caggagtgat gagcgatctt
     5761 cattttatca cagaatggac acagtattgt ttacctttag agactacaga acacatcgat
     5821 ttttcaaaaa aatgggaagc aggtcaacca gcattttacc gctttgaagc ggaactgtca
     5881 gaaatcggtg atacttattt ggaccttagt gagtttggga aagggatcgt ttgggtgaat
     5941 ggcacgaata ttggtcgttt ttgggaagta ggaccaattc tttcactgtt tgttcccgag
     6001 ggtttattta gaaaaggaca aaatgaaatc gttatctttg aaacagaagg tagattcagt
     6061 gaagttatag attttgtcaa agaaccaata gaaaaaagtt agaaaaggag aatggatgat
     6121 ggcaattatt ggagtaagaa ttgatggacg tttgatacat ggacaagtgg caaatctttg
     6181 gacgacaaag ttgaatattg gtcggatcat ggtcgtagac gatgaggtag caacaaatgc
     6241 gatcgaaaaa agtgggttaa agttggcaac gccagctgga gtcaaattaa gtattttacc
     6301 tattgaaaaa gcagccgcaa atattttagc aggtaaatat gattctcaac gagtgctgat
     6361 cgtagcgaaa aaaccagatc gtatcttagc gctagtagaa aaaggtgtac cgatcaaaga
     6421 attaaatgtc ggcaatatgt cacaaacaga tgaaacacgt tccatcacaa aatcaatcaa
     6481 tgtcgtagat gcggatgtag aggtcttcaa gaaattagat cagcttggcg ttcatttgat
     6541 cgctcaaatg gtgccaaatg acaaagcaga agactttatg agtcttttaa tcaaatagga
     6601 ggaatgattc atggatattc aatggtggca gatattatta ttaacattat atgcagggta
     6661 tcaaatttta gatgaattac agatttattc ttcaatgagc gccccagttt ttgcaggatt
     6721 atatgctggg ttagtaatgg gtgatttaaa agcaggttta atcattggtg gaagtatgca
     6781 gttgactgtt ttaggggtag ggacatttgg tggtgcatca aaaatcgatg ccaactctgg
     6841 aacgatctta gcaacagctt tctccatttc tctagggatg aacccagaac aagcgattgc
     6901 ggccatcgcg gttcctgtag caagtttgat gatccaatta gatattttgg cacgttttgc
     6961 gaatacgtat tttgcacatc ggatcgatca acatgtggaa acaatgaatt acaaagcaat
     7021 tgaacggaac ttcttgatgg gggcattacc ttgggcactt tccagaatgg ttcctgtatt
     7081 cttggcccta gcattcggtg gtggcttagt ccaaaatgtt gtggctgttt taaacggcga
     7141 cctaaaatgg ctaggagatg gcttatctgt tgcaggggca gtcttaccag cagttgggtt
     7201 tgcaatttta ctacgctacc taccagtaaa gaaacacatt tcttatctga ttttaggatt
     7261 tacgatcaca gcgttattag caacagtgtt ctcgaatgtc caattgttag gaacaagtgt
     7321 ggctagcgtg gtcaaagatt tcccaggaac attcaattca ttaccgatgc ttgctgtggc
     7381 attgattggt ctagcactgg caacgatcag ttataagaat gatcaaaaga atgaccaaca
     7441 tccagtagta agtcagcaat cacaatctga tgagggggaa atcgaagatg acgaaatcta
     7501 attataaatt gaccaaagaa gattttaagc agatcaatcg tagaagtttg tttactttcc
     7561 aattaggttg gaactatgaa agaatgcaag gttcaggtta tctgtatact atcttgccac
     7621 aacttagaaa aatctatggt gatgatacac cagaattaaa agaaatgatg aaaacacata
     7681 cgcaattttt taatacgtct aatttcttta acacgatcat tactgggatc gatcttgcta
     7741 tcgaagaaaa agaaggagtc tcttcaaagg aaactgtttc tgggatgaaa gcaggattga
     7801 tgggttcctt cgctgcaatt ggtgattctg tatttgcggc attgatcccg accatatttg
     7861 gagctttagc cgcaaatatg gcaacagatg gtaatccagt aggtatcctt atctggatcc
     7921 ttgctcaggt cgctgtcatg gtctttcgtt ggaaacaact agaatttgcc tacaaagaag
     7981 ggatttctct agttaccacg atgcaatcac gcttgactgc attgacagat gcggctactt
     8041 tactaggagt tttcatggtt ggagcgttag tagcaaccat gatcaatgtt aaattctcat
     8101 gggcaccaac gattggggat gtaacgttga atatgcaaaa ctatatcgat atgatcttac
     8161 ctaaactttt accagctgga atcgttggtg gcgtttattg gatgttaggt aagaaaaata
     8221 tgacacctac tcgagcaata ttcatcgtat tgatcgtatg tgtagcatta tctgcattag
     8281 gtgtgatttc gaaataaggg agaattggaa agatgagtag acaactagtc ttgattagtc
     8341 atggtaaatt ttgtgaagaa ctaaaaaaca gtacagaaat gatcatggga ccacaagaaa
     8401 atatctatac gatcccttta ttgcctgaag atggcgcgga cacgtataaa gaaagattcc
     8461 tctccgttac agcaccattg aaagattacg tagtgctttg cgatctgttg ggtgggacac
     8521 ctgcaaatac cgtttccaag ctaatcatgg aaggtgaaac gatcgaacta tatgctggca
     8581 tgaatttgcc gatggtcatc gaattcatca attcgcaaat gattggagca gaaccagatt
     8641 ttattggttc gggtcgtgag agtatcgttt cagtgaacca agtgattgct ggcatgggag
     8701 acgatgaaga agaataatca agcacaactt cttgactggt gatacaactg gatataaata
     8761 cggatgtata aagaggccgt gtataaaaaa gccaacgtac cgcgaaatat agttttctat
     8821 atttcacagt acgttggctt ttatcgtcat aaaatcagaa atgatctggg tcgatcgaag
     8881 aagctagtta caaataacta tttacgcttt tgctgtttac cagcattccg accattcttc
     8941 gcattgtttt ttggtgcttg tacttgtttt tttgtttgct tcgcaggttc agcaggtgtg
     9001 acatctttac gaggcgtcac cacttgtttt ggcggatttt gttttaattc ttccgcaact
     9061 tgcgctttta atcttggttt catgatgaca ttcgtaatga atgtttgtag acaactaaag
     9121 atcccaccga ctacccagta aagagtgaca ccagctgggg cactgatcga catgaagacg
     9181 atcatagcgg gtgatacgat caacatcgta cgcatcgttt tcttttgttc ttcagggata
     9241 ccgatagttg agatatagcc ttgaagtaaa taagcaatac cggcaatcgc tactaaaact
     9301 aagctagggt tacctaggtc gatcccgtaa aaggttgatt cacggatgcc ttctgtatag
     9361 cgagcagtga aatatagcgc agaaaagatc ggcatttgga tcagaagcgg taaacaaccg
     9421 ataccaccag tcatacttaa gccattttca cgataaactt gttgcatttc agcttgtgca
     9481 gccatttgtt cttctcgagt tgtcgcagct ttcaattttt gttgcgtagc ttcaacttgt
     9541 ggtttcaatg cttgcatctt ttcagattgg atcattgttt ttttcgattg actaatacct
     9601 aatggcaaaa tgatgatacg aacgatgact gtcatcaaga taacagccca gccgtaggac
     9661 caattaaagt tttccactaa atatgtaatc gcttgtccca ttgggtgaac caatatacgg
     9721 taaatgattc ctgaagtgtc agggttacca tttgaatcca tgcggacaca accggacaag
     9781 aaaagcatga tcgtgacaag cccagagccc aataaccaat ttttccattt attcataaaa
     9841 tacttcctct tttataattt ttacacaagc ttaactatac taaaaaagcg ctcataattc
     9901 aatcaacttc atagaaattt catagaaata accgattgga ggatcaaaaa agacgcttaa
     9961 tgttaaaaag aagaatccag ctaaaaatac ttcagtataa agcatactta gcacctccgt
    10021 tctctaaaat aagatgagca acggatattt ccttgaaatc ataattttag cgcaaaaaaa
    10081 agaagtggaa tgtcaatttt ttccacttct ttttttttac cattgagccg atccataaaa
    10141 aagctgactg ccatggtata aggggctaac tatccctgtg tcacttgttc taactctttc
    10201 attttctgga caaccagtgc tggtgtgatc gtttctttca tgaagtggat catttcatca
    10261 ggtaaacatg cacgttcagc tacggcttga tagtcacttt ctgttaaatg taaacccata
    10321 tcagctaaac tcgttggtaa gttcaagtga tgataaaacg gcagtaggtg ttcgatttct
    10381 gctaatttat tttcgatcat cagctggaca aggataccgt atgcaacttt gtttccatga
    10441 agttgctgat ggctatcagg gagtacggtc atcgcatcgt gaatggaatg agctccagct
    10501 gttcggccgt agtcatcgcc aaacccacca accataccag ccaataaaat gtttgtttcc
    10561 acgacatcga taaaagcttg attgattttt tgctggtcca tcgcttccat tgcctctgca
    10621 cttttctcta aaaggatttc gcggcatcgt aacgctgcaa attccgctac ttggacttcg
    10681 ataggcttga tagttaactg agagatgatt gcgtgagatt cataccattt tgctaaggtg
    10741 tcaccaatac cggcaatcat catctcgcga ggggagtgca aaatgatctt tggatcgatc
    10801 aacactaaat catttgcttt agggaacata tcatagcgaa gcatcactcc ttcttcatta
    10861 tagaccacac taagtggagt ataagctgcg cacgtcgcag ctaaggtagg taagatgatg
    10921 attggtaagt ttgtcttata ggcaatcaat tttcctagat ctgctacttt cccgccgccg
    10981 actgcaataa taccattgaa atccttcttt ttgcataaag aaacaaaact gtcagcaagt
    11041 ggatcggtac attctccacc ataatattca aaaatttttg ttattgataa atctggaaaa
    11101 acatctttta ctgctttcca agatgttttt ccatggagaa tcaaaacgtt gtcaatatgc
    11161 cttttgtaaa ggtgttcagg gagtgaatcc catgcgtttt cttttatttc atactcttga
    11221 ggggcaccgc gaacaattaa attttttgtc atataaaaaa cttcttccat tattttttta
    11281 aattaatctt tttttattat aatagttatg ctagtatatt agcaagttat ttatgaatat
    11341 ttattctttt atagcttttc tgattacatt attattcaaa ggtggccaaa tggatgaaaa
    11401 aaggaataaa catattatta gtggcgctga tgtttctact ggttttttta ccagtaaaag
    11461 tacaaggtgc agaaaaagat gaaatttacg agcggataaa acaaagtgga gaattagtcg
    11521 ttggcctatc tgcagactat gcgccctatg aattccatgc gaatgtggat ggtaaggata
    11581 aaatcgttgg ttttgatgta tccattgccg aaaagatcgc aaaggatatg ggtgtgaaac
    11641 taaaagtaga agaacttggt tttgatgcat tattaggtgc gttgaaaact ggcaagatcg
    11701 atttagttat atctggtatg gcggtgactg aagaacgttt acaagaagtt gacttttctg
    11761 acccatattt cgtggtacag caaaaagtac tcgttcgtaa agaggacaaa aataaattta
    11821 agacgacagc cgacttttct ggcttagccg ttggggcgca aaagcaaacc acacaagagg
    11881 agttagtgaa aaatgaactg actggtgcag agttgacctc cctgcaaaaa gttcctgatc
    11941 tgatcaataa tttgctgaat gaaaaagttg aaggtgttgt gctggaacaa ccagttgcag
    12001 aagcatatgc ccaacagaat aagaaagtag ctttgactcc agtaaaattt gcgcaaggcg
    12061 aaaaaaccac agctattgcg atgtcaaaaa atacaccaga actgaaaaat catgtcaatg
    12121 cgtcaatcaa aacgatcaat gacgacggct tgctgaaaga ttatcaaaaa caagcgaatc
    12181 aattgatgtt ccaagatgat cagtcctttc ttcaaaaata cggtccgtat tatttgattg
    12241 gaacaaaata cactatttta ttagcttttg tgggtgtatt gtttggaatg gtctttggta
    12301 gtttgcttgc actgatgaaa ttgggtaaga ataaattttt aaaggcatta gccattattt
    12361 atatcgaata cgtacgggga acacctctct tagttcaaat ctttatcgtt tacttcggaa
    12421 caggagtact gggattggat ctatcgaaac tggtagcagg ttgtattgct gtctcgttga
    12481 atagtgctgc ttatgtagcc gaaattgtcc gtgccgggat caatgcggtc aacaaaggac
    12541 agatggaagc tgcacgttct ttaggaatgg accagaaaga agccatgcgt ttcatcatct
    12601 tcccacaagc aatcaaaaat attcttccag ctctaggaaa tgagtttgtc acgatcatca
    12661 aagagtcctc agttgtttcg gtcattgggg tatcggaatt gatctttcaa acgggcgtgg
    12721 tacaaggtgc tagcttcaaa ccattcctac catatgtgtt ggtctctttg atttatttcg
    12781 tgttgacctt cagtatttcc cgtatattag ctttagttga aaggagaatg ggtctgaatg
    12841 cttaatgtaa aaaaactgtc aaaaagtttt ggcgagaatg aagtcttaaa atcaattgat
    12901 ctttctgtga aagaaggaga agtagtggtg atcattggcc cttcaggaag tgggaaaagt
    12961 acaattctac gttgtatgaa tctgctggaa gaaccaactt ctggtgaaat cttttttaag
    13021 ggacaaaaca ttacagcccc tgacagcgat atcaatcaaa ttcgtcaaaa aatgggaatg
    13081 gtatttcaaa gttttaattt gtttccccat atgaatgtac tcgaaaattt gaccatcaca
    13141 cctgtaaaaa tcaaaaaaga agaccctaaa aaagcaaaag aacatgctct atcattattg
    13201 aaccaagttg gtctaaagga aaaagtggac agttatccta ccagtttatc tgggggacag
    13261 caacaacggg tagcaattgc tcgagcatta gcaatgaatc ctgatgtgat gcttttcgat
    13321 gaacctacat ctgcacttga tcctgaaatg gtcggtgaag tgttggcagt catgcaggat
    13381 ttagctaaaa aagggatgac gatggtcgtg gtcactcatg aaatgggttt tgctaaagaa
    13441 gttgccgatc gtgtgatttt tatggctgac ggcatcattc aagaagaggg aacaccgaaa
    13501 gagatttttg aaaatccgca aaatagtcga acacaggatt tcttgaacaa agtgttgtaa
    13561 caaagatgga ggaagtaaga tggacaaaag taagattgca cgccggtttc agcaaccagc
    13621 agaaaatctg ttgatgaata ttgctatttt ggcaaaggaa aaagaggata ttatcgattt
    13681 atcgattggt gatcctgact tgatcactga tccgacgatt attgaagcag cttatacaga
    13741 tgtaaaaaat ggtgctacta attatactgc gcctgatggc agcaaagaat tcttgtcaac
    13801 ggtcgtagat ttttatcaaa agaaatatgg attgagcgtt gaatgcaacc aagttcgagc
    13861 tacagtcgga gcaatgcacg ggatgtatct agttttacag gcattactag atccagagga
    13921 tgaggtcatc atccatgaac cttatttttc accatatcgg gaacaagtca ttttatctgg
    13981 aggcaagcct gtttttgttc ctacctatga agaaaagggc ttccagttgg atgtggaaga
    14041 tttaaaagca gcgattacgg atcgtacaaa ggtgatcgtc atcaattcgc cgaataatcc
    14101 tacgggagct gttttttctg aaagcacgtt gaaaaaaatc gctgaagtcg caatcgaaaa
    14161 tgaactctat atcatttccg atgagattta tgaagacttt gcctttaaag agaagttcgt
    14221 accgatggcg cgatttgcgc ccgataatac gatcactgtc agtggtgtat cgaaggggtt
    14281 tgcgatgact ggctggcgct tagggtacat gatcgcacca gcatacatca atcaaattgc
    14341 tgggttgatc aatgaaaaca tcacttattc cgcaccgact gcttctcaaa gaggtgcaat
    14401 ctacgcctta aagcactatg aacagcttgt accgccagtt gttgaagtat tcaaagaacg
    14461 attagcttat attgagaaaa gagtggcaga aatcccttac ttatctttat cttctgtttc
    14521 tggaagcatg tatgctttta ttaatatcag taagagtggg ctcgattcag taacgtttgt
    14581 ggaagaggtt ttagaaaaaa gcggcgtact ttttgtccct ggaaaagcgt ttggggaaac
    14641 gatcggggac aactatattc gactagcagc gactcagcca ttggaaatct taggtgaagc
    14701 ctttgatcgt ttagcaaaac taaattttta gataaaactg tatatttgaa aacagctatg
    14761 ggtatccatt agaacaaaga gggaaaatga tcctttcgag agttggcttg ttgactgggc
    14821 agcgtcagga tttgacaatg aaaaagtggt agtggaaaat caaagtcgat tttccactac
    14881 cataaaatat tatagactcc attttattga accaaaattt ttgaagtcct cccgctttta
    14941 cgtatgccta tcactggtta ctaaactcgc taataagaat gtttttttgt cgatctagga
    15001 tggagagagg ggcaaacttt ttcgatctac atacagacag tccgattttt ctgacatttc
    15061 ctattcattt ctagaaggtc agcctattac aacaaacttt tttctgtcct ccatcatcgg
    15121 attttcctga aggtctacat attggacacg tccgctgggg cttggtgatc gtttgacttc
    15181 ttccacgaat tttttgattg cctcatcgtc gcccgcagct tcgatcgtga cagagccgtc
    15241 atcttcattt ttcactgttc cggtgatccc taattgatcc gctacgattt ttgtcatata
    15301 gcgaaaacca acgctttgta cacgtccttg cacgttcatc ttgattttgt tcattttatt
    15361 caccgttctt tcttttattt ttaaattaat gataccgact tttttgctac tgaacaagtg
    15421 caatgatcta atccatggta taatcagttg aggtgaaaaa tgtgaaagag atccaatcag
    15481 caaaaaatcc aagaatcaaa gaattaaaaa aattacataa gaaaaaatat cgtgaagaaa
    15541 aaggggaata tctgcttgaa gggtttcacc tgatcgagga ggctgttgcc tcaaatgcga
    15601 aggtcaacga actttttatt acgcaacgcg gtctgactga gtgggctagt tggcttgaag
    15661 aaaatcaggc gctttctgtt acactagtat cagaagaagt gatggcaact gtttcagatc
    15721 taccaactcc gcaagggatc atggcagtgg tggaagctga aaaaagagag ataccgaatt
    15781 ttgaaggtgg ttggttgttg ctcgacaacg tacaagatcc cggcaatgta gggacaatga
    15841 tccgcacagc cgatgctttt ggtctatctt gtgttgtact aggtaaggga acggcggatg
    15901 tctacagtac caaagtatta agaagcatgc aggggagtaa ctatcattta ccgatcatcc
    15961 aagaggatct aacaaaacta ttgcctatcc ttaaagagca aggatttata acctatggaa
    16021 cagaactgaa tgaagaagca gtgtccctga atgatatcca gacaacagaa aattatgcga
    16081 tcgtgatggg gaatgaaggt caaggcgtac gtccagaagt gctggcgcta acggataaaa
    16141 atgtgtatat accgattcaa ggaaatgcgg agtctttaaa tgtaggagtt gcagcgggca
    16201 tcatgcttta tcacttctcc gtatagttaa ggaactatta agctttctgc attttgcgaa
    16261 aacctcacta gaatattacg aacactagtg gtatactaag ttcaatttca aagataaatg
    16321 gaattcaaaa aggtggcaga tcgataatta tgacagaaaa atggcgtgaa gacgaagaat
    16381 atatgtctta tataaatgat ctattagaaa ctgatgcagt aaagaaatta gcaaattaca
    16441 cgcaacatgt ccactcgaca cgattagagc attcaatcag tgtgtcttac tatagctaca
    16501 aattagcgaa aaaatggggc ggtgatgcaa gagcgactgc tcgagcaggc cttttacatg
    16561 atttatttta ctacgattgg cgtacgacga agttcgatga aggtacacat gcgtatgttc
    16621 acccaaggat cgctgtgaaa aatgctgaaa aaatcacgga gctttcagat ctagagcgtg
    16681 atattatttt gaaacatatg tggggagcaa cgattgcccc tcctaaatat aaagaaggtt
    16741 atatcgttac ttttgtggat aaatattgtg cagtcaaaga agcagctcaa ccaatgacag
    16801 cttcaatgaa acaaaaatgg caacgatatt tcggtaaaga acaatcgatt taaggtagtt
    16861 aactaagtat ttgacccgag tattttgaaa aaaatcctcg ggttttttgt ttagacattg
    16921 actaattggt ctttttagtg tttactttgt tgagatgaaa aagtacataa ccaacataaa
    16981 aataaagggg gtgatgataa gatgcgatgt gtatcttatt taaaagagtt tgagggcttt
    17041 atctaaaggc actttgatag aaagatcact tttttcttca aagtagccga ttttgaaaga
    17101 agaaaggact caaagtcatg gaaaaaacaa tcaaaccata tcaatattat tttatcaagc
    17161 gagaagtcga gcagctgtta agcgcctata gttcagtgaa tgatccaaag acagtccaaa
    17221 cagtccaagc attagctgcg gaaaagatcc acgaggtact tggtcatcct aatacagctg
    17281 taaatgaatt tatggaaaag gtactagacg tcactctaaa aaaagaaaaa gcagaacgac
    17341 tattagagga attgaaagtg atcgttcttc cattcataca accgacaaaa ccgcagatcg
    17401 ataaattatt ccgaaaagtg aaaaagttaa aacaaccaga ttggaatcaa ctggatttaa
    17461 gggaacatac ttatattggt tggaatgatc caggtagcca aaaaaaattt atgatctatt
    17521 atgaagaaga aaaactaaaa gggatcagtg gaacattatc tcctacaatc aacaaaggaa
    17581 tttgttcgat ttgtcaaaga acttcaaatg tttcactctt tctttctacc acaaaaactg
    17641 gttcagatgg aacatacacg aaaaaaggaa attatatttg tcatgatagt gaccaatgta
    17701 atcagcagtt ggtacaatta gctccttttt acgaattcac agaacgagtg aaacgtgagc
    17761 ggtgatttca ttgtattttt ctgaaaaata tgctatctta tttggtgaag tgaataggtg
    17821 atggtgttcg cctttaaacg ttgatcgtaa gataactaat gacgcctact aaagaaacgg
    17881 ggctacccct ttttttagta ggcgtttttt tgaggaggaa atgagatgga aaatcgatta
    17941 cctgatccat tgaaggtgat catctatatg atcaaatggt tattgatcac agcaatttta
    18001 ggtattttta tgggaagttt atccgcattc tttctaacta gtttgactga tgtgacagct
    18061 attcggctgg agcatccttg gttacttttt cttttgccga taagtggggg actttttgct
    18121 tatctatata cgacgtatgg gggcaattct tcccggggaa acaatttagt gatcgatcaa
    18181 gggaatggag gaaatgaaaa gattccatta cggctgatcc cactgacctt atttggcacc
    18241 attaccacgc atctttttgg tggttctgtt ggtcgggagg gtacagcggt ccaaatgggc
    18301 ggggcacttg ctgagaattt tgctcgttgg ctaaaattga acaaaatcga gcgtgagatt
    18361 cttgtcatca gtgggatcag cgcaggtttt agctcagtat ttgggacacc cttggctggg
    18421 acattgtttg gattagaagt attggcaatc ggaaaagtga gaaccgaagc actgtttcca
    18481 agcttttttg cagcactttt tgcgaacttt gtaacggaaa gtttcaatgt caagcatacc
    18541 cattatcaaa tgggggcgat tcctgcatgg gacccttcac ttttcttcaa attgttgctt
    18601 gcgggcattg catttggtat cattggctgg ctctttagcc gttcgatcgt ctggttaaaa
    18661 gcttggtatg caaagcggat cccccatcca gttttacgca actttattgg aggagcagtc
    18721 gtggtactcg tagtagtgat tttacagacg caaagatatc tcggattaag cttaccttta
    18781 ttacaagatg catttaatgg aagtgctcat ccttatgatt ttcttgggaa acttggcttt
    18841 accgttctat cacttggcgc gggataccaa ggaggcgaag tgaccccttt gtttgagatt
    18901 ggggcaactt taggtgcgac attagcaccc attcttcatg tatcagtacc ttttttagca
    18961 ggattagggt ttattggggt gttctctgga gcaacgaata caccgattgc ttgctttgtt
    19021 atggggatcg aactttttgg aggtcaagca gccagctact tctttatgat ctgtttgatt
    19081 agctttatgt gttcaggaaa tagtggaatc tattctgcgc aaaaagttga agtgaaaaaa
    19141 ggaatcttgt tcctaccgat ccttggaaga atctcacgac aaaaagagaa ataaaaagag
    19201 gttgggacag aagtgtttaa ctccgagaaa taagaagaaa ttcacgaaaa ttgcttttta
    19261 aatttttgtg aatttcgctt atttccgaag gagttgcttc tgctcccacc gtttattcgg
    19321 agttagagtc tgtggcaaaa ctgattttta gttttgtccc acgctctttt ttattgagca
    19381 aatttgagaa taagagcaga aaatactgca aagccaatca ttaagccaca gatgataaga
    19441 tggcgttttt tgtgtaagac aaggatggca ataaattctc ttaagatagc attcggtaag
    19501 tagtatagtg cagtttttgc accgattccg tttgcattga gtttcgcttg tcgcgcatat
    19561 agtccagctc taaagatatg gtaattattt gaagtaaaaa tcgcgcgata aggcccattc
    19621 atttgctgat ccattatttc ttttgaaaac agcatatttt ctagcgtcgt tgtggaattc
    19681 gtttctacta aaatatcacg ctcgggaatc cctttttcta aagcatattg tttcattgca
    19741 atggcttcag ctactttttc atcggttcct tttcctcctg acatgagcaa gactggcgga
    19801 ttcaaagttg ctttactttg tgcccaataa aatgcgatcg ccttattgat ccttttagca
    19861 agtaggggag atacgcgttc accatcaatc aagccagcgc cgagaacaac gatataatct
    19921 tggttatacc ttggacggtt gaactgatac aaaattgaaa cagtcataaa attaaagaaa
    19981 acgatgagta gataagcaaa aataaacgga atgacagcaa atagagcatt tgcccattgt
    20041 gggagccaat ggactaatgt atagtccaat atcaaaaaga tagtcaaagc gactcctaac
    20101 aagagagtca aaaggttggc caaagaacgg gattcttttt tcatgactac aacaccattc
    20161 caaaataaaa aaatgatcaa aatgtagata ccgattgcca aaagaattaa tattggtatc
    20221 ccgatcaaag ccaataaccc accgataaag atattttgcg tctctattag tagccaccct
    20281 agataaccac caaagacaat caaaaacgta ttaaaaagta cgccattgat taacctacgt
    20341 ttttccttaa aatagaaaaa tgcaaaaata ctgaaaaata gcaatggaat caaaaaataa
    20401 aatgtatgat aaaaaacttc taaaacgacg aagtaagtga ccatcatcat aaaagtccac
    20461 gtcccacaat aaaagaatag agcgggatcg ctttggcgct tgcgccaata gaggatgcct
    20521 gacattccta aaatatagat aactaaaaat atcaggtctc tagtaagatt cattgaatga
    20581 actcctttcg atttgtaaat tttttcttaa aaaagatagc atcatttagt agtaaatata
    20641 tcgaatatag aaaagttagt tactaaatag tcaatcagtt ttttcacgac gtttctatta
    20701 taacgaatta tgttgaaaaa agcttttatc gaaaacataa atcttcaaat taacagattt
    20761 tttagacctc gatcatgatg aagacttttg tagatacaaa taactgtaac attgaaaatg
    20821 aatgcgaaaa cgatattttt ttttattgga aaatatcggt caagcaataa accagttgca
    20881 taataaaatg ttaacggcaa caaaatctgt ggaaggaatg aaccggatga tttgatggaa
    20941 aaaacaagat agacaagaag tatacatagt aaaacgtgga acaagtaggc cagaatgaaa
    21001 aatcagtttt tatccactcg cttttctata cattcactgt cacaaatata atcgaaagta
    21061 attctatttt gagataaagt attttatagg agatagtata tgaacaaaaa aagatttaaa
    21121 cgtattgaaa gaaaatttgc attaacttat gaccaagcta agtatttgaa aacactattt
    21181 ccaaaacagg tagttcggga taaatatagt gatcatttta tttcttcctt gtattttgat
    21241 acaaacgatc atcacttttt aaaagaaatc actacagaaa agtcctatcg agaaaaattt
    21301 agaatcagaa aatatggaga tcctcaggac atatcgccta tctacttaga gataaaaaga
    21361 aagtttcaag gaatgacaga taaacgtaga ataaaattag cgcatcatga gctcgatgat
    21421 atgttggaaa atatcgaatt ggcagaaacc acattcagta atttagaaga agagtatatt
    21481 tttaatgaaa tacagtggtt gttcagaagg ttggcattgg agcccaaagt atatatctat
    21541 tatttaagag aagcatttgt aaatagagat tcagaagatt tgcgaatcac ttttgatcaa
    21601 aatatccatt accgtctaat gaacttaacg acctgggaac actgtacaga agacaatctg
    21661 gtagcgccag aaatcgatgt gctgatggaa gtaaaaacga caggagaaat acctgaatgg
    21721 ttacaggtag gattggatag gatgggtaaa aaagagataa aattttctaa atataaacaa
    21781 gtctataaaa gatatatcca agaggacccg ctctatgttc aatagtgtct taaaaaatga
    21841 gccttctcaa ctcacactag atgatttctt tttatgtatg gtggtgtcga ttttgctagg
    21901 cattctgatt gcttacaccc atatgtatca aaacaactgt acaaaaaact tcatgtttgc
    21961 cttgattggg ttacctactt tagtacatac cgtcattttg ttgatcaacg gtaacttagg
    22021 cacggggatc gctgttctag gcgcatttaa cttagtgcga tttcggtctg ctcaagggaa
    22081 ttcaagagat attttcagta ttttttgggc aacgtcgatt ggtttagcca cagggacggg
    22141 atatattgtc tatgcactat tcgtgtctag tatagttgct gctatccaag tcatcatctt
    22201 cacgcttcca atcaaaaata aacatacagt ttttaatact gaaaaaacga tcaagctaaa
    22261 agtttctaaa tccttgtttc tttcgagtat tattgaagaa aaaattgcag aatatacaga
    22321 aaactgcaat ttaaaaaaag taaaaatcaa taaagaaact tgttccttac actatatagt
    22381 aaaagtaaaa acgagtatgc ttgaaaaaga attggttcga acgatcatgg atcatccaga
    22441 agtcatttcg atcactgtag gtatggataa atcgaataaa ttattgtgat aaccaagaaa
    22501 gtacttaaca atgaagtaag accccagttc caaagtttag cggggacact tcaataaagt
    22561 actttctttt ttgtcttcag caactactgg taaaactgag agcaagcttt cgtgagagta
    22621 cagcgataaa gatcaacctc cctttttatc tcgagcaatt tccttgcaag gctgtttcgt
    22681 tgatcttaaa cgagttagca acagtcaaag atgcgatggc cataaaaaaa ttgcattaag
    22741 ttggctggta tctgatagct gggaatctaa ggaagactat ccattctgtt tcttaaattc
    22801 cgctagttga tagatatcag ccaattgttc cagatgatca acatcaaaga taaccgaaag
    22861 atgtttcctt ttattcccat ttgcttgaag agtttttatc ttctctttgg ttccttcact
    22921 caatgagagt gtgactaatt tctccctcgt tttttctggg aaatgaaagg atactttgaa
    22981 aaatccgtcc caaatagaaa gccagaagat cggttttact ttctgagagt gggacaaaag
    23041 taagaaacac ttttgtctca ttctttaaaa acgaataaac ggtgggagca gaagcaactc
    23101 cttcggaaat aagccaaaat tcacaaaaat ttgaaaagca attttcgtga atttcttctt
    23161 atttctcgga gttaaacact tctgtcccaa cctcttgtat tcaccttttg atagccaagc
    23221 ttttccatca ttataaaacc gcaaatttca aaaaaaaacg aacaatacaa aaagcgtatt
    23281 gctcgttttt ctcttcagtt tctcacacct aaacgacttt tgtctcgacc tctttttggt
    23341 gactattcat gatgttaaaa taaattactg aaaaaatctg tgacatggcg ccaaccaatg
    23401 acaaaaatat tagctttttc aacagtagaa gtggtaatga tgtctgtcga aggctcttgg
    23461 tttgcttcca aatagcctaa tttgtcatca gctaattgga tcgttgctga gccaactgta
    23521 gtgcctttgt tgataggagc ggtcacttgg tcatccgtta ctttcttttt atcaattgtt
    23581 ggagtaatcg ttaattttcc agtatccatt ccattttcga cccagacttt tacagggctt
    23641 tttagcgcaa cattgacgga ggtttctttt ccatctttca ctttgatgtc ttttaggctt
    23701 gggatgcttg catttgcttt tccaagttct ttttctgacc agttgtcata acaataatcc
    23761 atcaatcgtg atgtttcatt gaatcgggca cttggatttt cttcatggtt atctgcattt
    23821 aaaacgactg tgatgattcg tcggccatct ttgttgatcg ttccgacaaa gcatgcaccc
    23881 gcaaaatcag ttgtgccagt ttttaatcca tcgacacctt cgtgatagtt tacaaaacca
    23941 ggtagcatcc agttccaatt gaccatttca accggtgact gtgtgttttc gccgaatgtt
    24001 tgatttgtgg tactcgtgat ttttaagact tcaggataat ctaagatgag gtgacgagca
    24061 acaatagcga catctttcgc agacagttga ttttcatctg tttcaccagt attctcaggt
    24121 cgattctcac ctaggtaaga attgtttaaa cctgatgcat tgacgatcgt tgcatctttg
    24181 ataccccaat cttttagttg tttcttcatt agctcaagaa atttactttc actgcctgca
    24241 actttttcag ctaaagcaac catggaagca ttggctgatt ggatgaaagc tgagtcaaca
    24301 agttctttca ctgtataatc attttcttta tgcaaaggaa cattcgatag atccggcacg
    24361 acacttaatt tttctgcata atccgaaatg gctaccttat cttcccaaga taatttaccc
    24421 tctttgattt gttcaagtac gatatataga ccaatgatct ttgtgatgga agcaattccc
    24481 atcgtttttt cgccatcctg atcataaagg atttttccac tttctgcatc tacggcaaag
    24541 gcagctttgg catttatttt aaatgcgtcg tctgcttgaa cagttgcgct aggcaagaaa
    24601 gaagtgatac ataagataag acttgctaat agtacaataa aacgggtatt ttttttgaac
    24661 atctttgttc tcctcatttt taaattgatt cttacaactt agaatattat cagagagaac
    24721 aaaaggtttc aatggttctg aaaaaaacct tataaagaac aagtcttatt aatgaagaat
    24781 catagaaagt tatcttttgg gttcattgac acgaactttg actggctgaa cttcgggttc
    24841 ggttaggcgt gatgctgcaa aaagggcgcc tgctgagagt gcgaccaaac taaaaatcat
    24901 gaataattgc ataagatcac ctcgttcttt tttgtttttt tatagtatag caagagagtt
    24961 tgaagctact gttaaggaaa tgagcatata attgatgaaa gtatgaaaaa aatatgaagt
    25021 aggacaacac aactaaaaat cgatcaggta tttctctggt agggattcgt tttattgtat
    25081 tgataaaatg ggatgtggta gactttattt acagatacaa agaaatgatt tttttaggag
    25141 gggtacgttt atggcaaaaa aagaaaaaga attattatca caattgactt cagaaggtac
    25201 caaaggaccg tttgtcacga ttatgttaaa cacccatgtt gcgcatcaag acgtagaaaa
    25261 agatcaaatc aaattcaaga attttgcaaa ggaagcgaaa aagcgctttg aaaagaaata
    25321 ctctgagcat gattggtctc cattccaaga aaaaattact agtttgttgg acgatccgtc
    25381 tttctggcgt agtgccacaa cgagtgtagc tgtcatagta tcaccagaac aagtgtttat
    25441 ccaccgtttg ggtattcctg tagatgacca gtattatgtg agtgatctac cttatttatt
    25501 agcgatcatc aaaaatggac atttcaacta ttcatactat ttattaggat tgaatcgtga
    25561 ttcgatgaaa ttgtacgaag taacgaataa acaagtcaaa gaagtggctt tgcctgaaga
    25621 tgcaccaaga gatgtcgtca ctgctttagg atctgaacta acaggaggcg ggttgaacta
    25681 cgcatcaaat ggtagtgaag gtacgacgtt ccatggagtc aatcctaaag atgaagaagt
    25741 cgaaattgat tgggtcaatt attatcaagc agtcgatgac tatttgaaag atacatttga
    25801 aaatccagag aaatggccta tcttcttatt tgcattacca gaaaatcaga cgatgttcaa
    25861 aaaagtggcg aagaaccctt attatcgttc cgatgttgcg atctcagctt cacctgcaca
    25921 actttcatta aaagaaattg aagcaggcgc aaaaaaacta tcgcaagaat tagctgtcaa
    25981 agaagcaagt gactacaata agttgcttga taaaaagttc attgatcaac tcgttgatat
    26041 tataccggca gcaaaagaag ggaaaatatc ccatttattc attgctacat ctaatttagc
    26101 agatggtttt ggcgaagatc cagaaacaga atatgaccgc cgtcaggtgc tgaatgaact
    26161 agcagataat gtgatcacga acggtggtca ggtctttatc cttgaacaaa aagatgcacc
    26221 agacgaaaag agtttagttg cgatcttacg ttattgagtg aaaactacca aagtagatga
    26281 atggtcaggc gttgatagaa gtgtatagct ccgagagaag gagtttcctc tgtttcagcc
    26341 gttggattca tcttgagcga gagacaaaag ctagcttttg tctctcgctc attatttttg
    26401 tgctttagtc ccattggctt tgcgagggga agatactttt ctcaaaaaaa cgaaccttgc
    26461 agttgagagg gggatagatg cgcatactaa tttttatcag ttaaaaaatg atgtggatca
    26521 ttagagaatt ccaaagttcg ttcatccttt ttcagataat aaattgaaat tttttgcaaa
    26581 agtgaaaata gcaaatagaa attttctgaa tcttataggg gtgatttcga gaaaacgttc
    26641 gtttatttgc aaatatatct tctaaataag agaataaata caaaaaaata aggaaaaact
    26701 ctggcaattt tgtaaacggt atgatatagt aagaatgtag tttttgttcg gtaatctgtg
    26761 gtagcttgtt tcaaataaac atcttccaga tatacaccat tcaagtaatt gcaaaatatg
    26821 tgttgataca caaggaggat ttttattatg gaaacaggta cagtaaaatg gtttaactca
    26881 gaaaaaggat ttggattcat cactgcagaa aacggaaacg atgtattcgt tcatttctca
    26941 gctatccaag gcgacggatt caaaacttta gaagaaggcc aagcagtaac tttcgacgtt
    27001 gaagaaggtc aacgcggacc tcaagcaaca aacgttacta aagcttaatt tacctatatt
    27061 aaaaataaaa gtccagacat ttatgtgtct gggctttttt gtcttttttt ccttaaaaag
    27121 cgatgagagt atttttctgc actttgtagt ataatttcaa aagaacatta gaggcaggag
    27181 aagaggaaga tattttgaaa ccaagaactt atgcagtcgt tgatatcgag acgactggga
    27241 cgaatccaaa agaagatcgg atcatccaat ttggctgcgt catgattgaa gctggtcgga
    27301 tcacttcacg tttttcgatc gatattcatc cagggcggaa gatttcaaaa caaatccagc
    27361 atttaacagg gatcacgaat caacgcgtcc aaaaagcgcc ttattttgaa gatgtcgccc
    27421 aaacgattta caatcttttg gcagacacca tatttgtcgc gcataatatt tattttgatt
    27481 ataatttttt gaaccatgaa ctcatgcgat gcggacttcc tagtttgaag ataccgggca
    27541 tcgataccgt agaacttgca caaattttct tgccgacgga accaagtttt cgcttagctg
    27601 atttgtcaga aagccttggc tttattcatg agaatcctca tcaggcggat agtgatgccg
    27661 aagtgacagg tcagcttctt ttactgatcg aagaacgaat gaggaaattg ccgatcatca
    27721 cattagagca gattgcacga ttaagtagcc atacaggaat ggatacgagt cgtttcattt
    27781 accacgtgct tgaagaaatg aaagaaaacc cggagcccct tgatctctca ttagaaatca
    27841 tcgatgggct agctttgcag aaaaaagaag tggagctatt cacatccgtc cattatggcg
    27901 agcggacata tccaagaaaa aaacaagcaa aagaaaaaat gtttggtaaa acactgatgt
    27961 atcgaaaaga acagaacagg ctgatgaatg ctgtctacga ccatttcaca aaagatgaat
    28021 caaaagattt gatgatcgaa gcagctacag gaatgggcaa aacgattggt tatctgttgc
    28081 ctttgagtta tttggctaca cctgaaaaac cagctgtcat cagcaccgtt tcgcttgttt
    28141 tgcagcagca gatcatggaa aaagacattc cattactgaa tcaattgcta gatcagccga
    28201 tacaacctgt gattatcaaa agctatcgcc actatattga tttacagcgt tttaaaggta
    28261 cacttgtgga accgccggga caaaaacaat atgctttgta ccaaatggct gtcttagttt
    28321 ggctaacaca aacgaagacc ggtgatttgg atgaattaca tttaaccaat ctgaaccatg
    28381 cattctttgc agatgtcgcg cacagaggca ctgggttttt agcaaggaat cagtcatttt
    28441 acaaacaaga ctttgtgcgt ttcttgcaaa agaaaatcaa acaaagcaat tttttgatag
    28501 tcaaccatgc atttttagta caagaaacac agcgaaaaga accattgctt ccagctagtc
    28561 cttatttgtt gattgatgaa gtgcatcatt tgccggaaat tgctgaaaaa gtaaatgaca
    28621 aacgactaag tattaactac ttccataaac aaattcagca actggaagag ccgaatcagt
    28681 tatttgaccg catacaaagt tatttgccga aggaacatga agccttgcgc tggttgtcga
    28741 tttatcgaga agaactctct gcactatgcg aagcgcagat ttgcgtggca gagggattaa
    28801 tgaacttagt aagcagcaag caacctaacg gacaatatcc agaagaagtc atgatgacaa
    28861 aagaaatgtt ggatcagctg ccactggaat ctggaacagc aatggatcat ctgcaacttt
    28921 tttatcaaga gatccaacaa ctacaatcac agatgcgctt gacttttgag attatccaag
    28981 aaagttggac caaccagatg cggatcgatt tagcaagttt atatagctta tttgatcaat
    29041 tagacaagca atacttgctg atggaccaat ggctagaaga ctggcgagat catttgatcc
    29101 attggttcgt tcccgggaat acagcacagc agatgatttt tcatctcctt gattttcaag
    29161 cagctatgtt gccgtcaact gtgtggtatc ctcgatatga ccatattctt tatattggag
    29221 gaacactgaa gatcggacct aatcgacagt atctctctaa acgacttggc attgaagcag
    29281 cacctcttaa agtgattgct tctccttatg attatgagaa acaggcaaga atctatgtgc
    29341 cgaaagaagc acctgcagtc catcaagtca gcaatcaagc atatatcgat tatttagaag
    29401 cagcactcac aggattgatt gaacaagaag atcggccgat ccttgtttta ttcacttctc
    29461 atgatgtttt gcaaaaggta tatcagaaaa tgcatttgtc tatgctagaa aaaggacgtg
    29521 aaattcttgc tcaaggattt ggcgggagtc gggaaaaact tttgaaacgc ttcattttgt
    29581 cagaaaaaag cgtcttattt ggtgctgaca gcttttggga aggcgtagat ttacctggag
    29641 atgctttgca gattgttgta gtcacacgtt tacccttcga caacccgtta cgtccgatgg
    29701 tcaaagctag gaatgcttat ctggaatcag aggggatcaa tcctttttat caggaatccg
    29761 tgcctaaagc agctttgaag ttacgccaag cattaggtcg tttgataagg acggagacag
    29821 atcgtggcgt attacttatt ttggatcgga gattagtcac tgcaaaatat ggtgcacgaa
    29881 tcatccatgc attaccgaaa aaagtaccga taaaagagct agctatagat gaaatctatc
    29941 aggatatcca tgaattctta aagaaaaatg aagaaggaag ttaaaggtgc cgtctttttt
    30001 catcattaaa atctgctata atgaaaagga atgaagataa aggagcgacg acccttgaag
    30061 aaaaaacaga caaaggaaaa aacgattaat cgcgtgttgc ttggcttgat cgtattttta
    30121 ttagcggtca tcgtgatcag ctcgattttt tacttgcgtt ccagtcgtcc ggcggtccag
    30181 gcgaaaaaag aagcagtaga aatcgcaaag aaatatgcaa atctcgagga agccgatcat
    30241 ttttactggt ttactcgaaa gaaaacgtac ttttcattga cgggtacgaa tgataaagga
    30301 aaagaaatcg ccgttatcat tcctaaatct ggtgaaaaag taaaagtatt ggatcaaaca
    30361 aagggactga ctgaaaacga agcaaaacag acaattgctg cggcccatcc tgaaatccaa
    30421 atcgaaaaag ctgctttagg gatgtatgat gatcagcctg tgtgggaagt agtaggaaaa
    30481 gaaacaaatg ggaaactgaa ttattacctg attggctttg aatctggaga tgaagtcaag
    30541 acaatcaagg gaatttaaaa ggtaaaggaa tgaatgggat ggagttatca aaaagaacaa
    30601 aacgattaga accgtctgtc actttggcag ctgcggcaaa ggccaaggca ttaaaagcaa
    30661 aaggcagaga tgtgctgagt ttgactgtgg gagaaccgga ttttgcgaca ccagaaaaca
    30721 tccaagaagc agcaatcgaa gcaatccgca atgggaaagc tagctattat acaccaacag
    30781 caggaatccc agagcttcga caggcaatcg ttgattatct gaagaaaaac tatggacttg
    30841 tctatgaacc ttcacaaaca gtcgtaacag acggagcgaa atttgctttg tatacccttt
    30901 tccaaacgat ccttgacccg caagatgaag tcatcattcc tgtgccttac tgggtcagtt
    30961 acggtgagca agtaaaactg gcagaaggac ttcctgtctt tgtgaaaggg gaagaatcca
    31021 atagctggaa agttactgtt gagcagctag aacaagcacg ttcagaaaaa acaaaagccg
    31081 taatcatcaa ttctccatcg aatccgactg gaatgattta tagcaagaat gaactgcagg
    31141 caatcggtga atgggcagta aaaaatgata ttttgatagt tgctgatgat atttatggcc
    31201 gtttagttta taatggcaat gagtttactc caattgcaac gatttcagaa gcaatcaaaa
    31261 atcagaccat catcatcaat ggcgtgtcta aaacatatgc gatgacaggc tggcgcattg
    31321 gttatgcggt ggggaattct gagatcatca atggaatgat tgctgttgct tcccagtcaa
    31381 ccagtaatcc gacagcagtc agtcaatatg cagctgttga agcattgaaa ggcgagcaag
    31441 atacagtgga agaaatgcgg aacgcctttg aagaacgcct gaatacgtta tatccgcttg
    31501 tggcagaact gccgggtgtt tctcttgaaa agccacaagg agctttttat ctttttccta
    31561 atgtaaaaga aacgctacgc atgtgtaaat atgaaaatgt cacacaatgg gtagaagatc
    31621 ttttagaaga aacaggggta gcgcttgtga ctggcgaagg ctttggggca ccagaaaatg
    31681 tccgcatgag ttatgccact gatcgcgcta cgttagaaga agcggtacgc agaatcaagc
    31741 aatttattga gagtaaaagt caaaattaat cctgaagaaa ttcaaggaat ataaaaggag
    31801 agtcatacgt ggaacaaatt caaatcatcg attcgaaaaa ccatgtggga gaaacagtaa
    31861 caatcggcgc ttgggtcgca aataagcgat caagcggaaa aatcgcattt ttgcaattaa
    31921 gagacggaac agcctttttc caaggtgtag ttgtaaaaag cgaagtgccg gaagaagttt
    31981 tccaaacagc taaaaatcta caccaagaaa catccgtgtt agtaactgga gaaatccgtg
    32041 aagacagtcg ttcaaaattt ggttatgaaa tcggaattac aaatgtggaa gtggtaagcg
    32101 agagccacga ttatccgatc acaccaaaag aacacggaac agacttcttg atggatcatc
    32161 gtcatttatg gctgcgttct tcaagacaac atgcgattat gcagatcaga aatgaaatca
    32221 ttcgtgcaac gtatgaattc tttaatcaac atcattttgt caaaatcgat ccgccgatct
    32281 tgactggttc tgcaccagaa ggcactacgg atctattcga aacaaattac tttgatcaaa
    32341 aagcttattt atctcaaagt ggccaattat acatggaagc tgctgctatg gctttcggaa
    32401 aagtcttttc attcggtccg actttccgtg ctgaaaaatc aaaaacacga cgtcatttga
    32461 tcgaattctg gatgatcgaa ccagaaatgg ctttcactca tcaagaagaa agtttagaag
    32521 tacaggaaca atatgtagca ttcttagtac aaaatgtttt agatcattgc gaatacgcat
    32581 tagatgtact aggacgagac aaagaagtct taaaaactta tacaaaattg ccattccctc
    32641 gtattactta tgatgatgcg atcgaattac tgaagaaaaa tggatttgat gacatcgaat
    32701 ggggcgaaga ctttggttca cctcatgaaa cgttcattgc gaattcattc gaacagcctg
    32761 tctttatcct aaactatcca aaagccatca agccattcta catgaaacca catccaacaa
    32821 gagaagacgt ggtgatctgt gcagatatga tcgcaccaga aggttacggt gaaattattg
    32881 gtggaagcga acgggcaaca gactatgatt tcttgctaga acaaatccgt aatcacggtt
    32941 tggatgaaaa agaatattca tggtatcttg atctacgtaa atacggttct gttccgcact
    33001 ctggttttgg tctaggcctt gaacgaacag tcacttggat tgctggtatc gaccatgtgc
    33061 gtgaagctat tccattccca cgtttgttga accgaattta tccataagag gttgtgaaat
    33121 cagtgccttg acctgagtat tagagaaaca aaacggaaaa atagcttctc atattttact
    33181 gttttttgag ctaatatcga gggtcagctg attgaacacc gtttatatca agaggttgtg
    33241 cgtctgccgt tcagctccga gtcacaagga gtaatttcaa aaaaacattt tttcatgttt
    33301 gatgactatt gtgacttgtg accaaggagt tggccggaga gcaacgttta tttaaaaaaa
    33361 agaactgcga actctcgcag ttcttttttt ttaaatacgg gataaaggaa gtttccagca
    33421 gataagagaa gaaagcaaaa gaaattgcta tttgattcac aaggttctat aacgaaatcg
    33481 caatcaatca aacatataca aagaaggtta ttcaaatgga aatctgattt gatgatgctt
    33541 ctgctgttga aataaagcag catctagcac ctgaaaacaa actaatagta acattcgaag
    33601 atggagtagg tccgtatttt cagcatgcaa tgatccatat gtaagttcaa ttcacccttg
    33661 aaatcatcgg accagataaa gttatggcca atctatccca agcaatctag aagagatatt
    33721 agtaaaggaa catttactgg aagtcttaga tgagcctata aatatatgtt ctgatggcgt
    33781 catcaagtgg tctgattgac ggaagcttag gtgtgatcga tctctctgga ccaaacaatg
    33841 aaggaatcaa ggggaatcca gcacgttaag aaaaaaatag cataaaaaac tagacacttg
    33901 atgagcgttc attcaattgt ttgaagggac taggctggtg tcatttttta tacatatatt
    33961 ttctctttcg tcttgatagg gcttatggga taatataagg aagaaaatca tcgttttatt
    34021 caaaaaggag tttttgacaa atgaaaaaac gtatattatt tatcggtgac agcattacag
    34081 acgcaggaag agaccgagca gaagcaactg atctaggtaa aggttatcca cttttgattg
    34141 ccggaaaact aatctcccaa tatccaacga tggaatttga atgcatgaac cgtgggatag
    34201 gaggagacaa aattgcagat ctgcaagtac gctggcgaga agattgttta gctttacaac
    34261 ctgactatgt atcgattttg atcggaatca atgatacctg gcataatact agcgatcagg
    34321 ctatattcgg aactgagaaa tctgctcagc aatttgaatc cgtttatcga aaattactaa
    34381 atgaacttca aaaagcggga atctgcaaca tcatcttgat ggagccgttt gtacttccta
    34441 taaccgacga ccgtcatggc tggcgtaaag acttagaccc taaaatccaa attgttcgta
    34501 aacttgctaa ggaatatggc gcattgctgg ttcctttaga tggcatattc aatgctttag
    34561 ggattgctta tggtttttcg aaatatacaa gagaagatgg ggtccaccca acgattggcg
    34621 gacatgagat cattgcacaa agctggatag atgcaattgc cgcagtcgat ttttttagtg
    34681 gagataaaaa tgagcgaaag aaaagtgaga aatgatggaa atacggtggg cagtaaaaga
    34741 agatctacct caaatcaatc agctgattac tgatcatcca gaatcattcg taaaagcaaa
    34801 aacggcagct gacctaaatg ataagaataa actattgctc gttgctgtaa caaaagaaaa
    34861 acggattatc ggttttgcgc aagcggacgt gcaacagtca gaagaacggg tgggtcagcg
    34921 accatcagga aaactaatcc aacacacatt agtaacaaaa gaaaatacgg aagaaatcca
    34981 aaaaaaattt gcagaatatt tgatgctgga agctaaaaaa agaagaataa aatcaatttt
    35041 tgcccagttt taatcaaaaa cagtaataga aggagaaata caatgagttg tttaggaaat
    35101 gtcatctggt ttatattcgg cggactaatc ggtggtctta gctggttagc agcggggata
    35161 ctttggtgta tcacgatcat aggtatccca atcggtatgc aatgttttaa gatcgccttc
    35221 ctttcttttg cgccttttgg caaagaagta atttatcacc agtctggcat gtcattgatc
    35281 gttaatatca tctggctgat cgtttcaggc ctaccaattg ctctcgcaca tgtcgtcagc
    35341 ggcatccttc tatgtatcac gatcataggg ataccttttg ccaagcaatc tttcaaactt
    35401 gctcgattgg cgttgatgcc attcggtgca gaaattgtat aaaagagaat aataaaggtt
    35461 agataaagca tagagtcagg tgggatacat gtatttcacc tgactctatg cgttttcctg
    35521 tataagttct tccatgtgac ttttacgtct tttatttata tggggttact gtttatggaa
    35581 agttgaaata attttcatta aagaactctt tctatatttt tgaaagcgta gtggagcttc
    35641 gtatatctgg gaaggacatt ttgcagggat tgaaaaaaga tattgtccta tcgaaaaatt
    35701 aatgaattta gacaaattgc cagcaacagg cttcaaactt agttgctatc ctgtcaagat
    35761 caagaaggca agtgcgggtt ggataagagc agtggcaatg atcgaagaga aaaagaaaga
    35821 ataagccaaa acatctgtat ggaagaaatt ttccgatgca gatgtttttc attttttctt
    35881 tcggatacct tgacatatga ataaccattc atttataatt agttatgaag agatattcat
    35941 atgttgaggc gaggaggtca ttaaaatgaa aagttacaga ttagaaggtt tagattgtgc
    36001 gaactgtgca atgaaaatcg aaaaaggagt tcagaaaatc aatggagtca aggaagcaac
    36061 ggtaaacttc acttctggaa aactaacgat tgacgcggag gaagatcatt tggctacgat
    36121 tgaacaagaa acaaaaaaag tagtgaagga attagaacca gatgtcaaag taacagaaat
    36181 agataaggaa aaagtatctg aacatggaaa tgaaaaagag agaaatacgc tgttccggat
    36241 tttattttcc cttgcaggta tcgctttatt gctattattt gattttaatg aactaattcg
    36301 cttgataggg tatttattga tttatctgct gataggatat gatgtagtga agaaggcagt
    36361 gatgaatatc gtcaaaggaa aaatctttga tgagaatttt cttatgtctg ttgctacgat
    36421 tggcgctatg ttgatcggtg aatatccaga agccgtagca gtgatgctat tttatcaaat
    36481 cggcgagtat ttccaaggat tggcagtcag tcattcaaga aaatcgattc gcgaattgat
    36541 ggcgatccgt ccagaaacag ctcacgtgca gacggcggaa ggattgatga ccgtgaaccc
    36601 tgaagacgtg ctgatcggtc agtttgtact tgtgaagcct ggtgaacgtg taccgttaga
    36661 tggaacgatc atcgaaggag aatcattagt cgatacgtca gcacttacag gagaatcagt
    36721 ccctaaatct gtatatgttg gtgaaacagt attaagcgga tttatcaata aaaataaacc
    36781 atttttagtt caagtagaaa aatcttatga aaattctact atcagcaaat tattagaatt
    36841 agtagaaaat gccagcagta aaaaagcacc agctgaaaat ttcattacta aattcgcgcg
    36901 ctattacaca ccagtcgttg taggattggc cgttttatta gctgttttac ctccacttgt
    36961 tgtttctgga gcggcgtttt ctgaatggat ctatcgagca ttgacatttt tagtgatctc
    37021 atgtccatgt gcattagtga tttccgtgcc acttagtttc tttggcggta tcggaggagc
    37081 aagtaaaatc ggtgtattag taaaaggttc aaattacttg gagttgcttg cgcaaaccga
    37141 gacagtggta ttcgataaaa ccggcacatt aacgaaaggc gacttttcga tccagacgat
    37201 cgatacaaaa atcgatccaa agatattcat gcaatatatt gcgagtgcag aacagttttc
    37261 tacacatccg attgctcaat ctgttttaga aggctacgca ggtccgcttt taccaacagc
    37321 taatatccag gaatttgcag gtgaagggat cttagctgaa gttgatggca aacaagtatt
    37381 agtaggtaat cacaaattga tggaaagatt tgaaatctct tttccttcaa gtcaagaaat
    37441 cggtacattg ctttatctag caatcgatca aagttacagt ggttatcttg ttattgcaga
    37501 taccttgaaa gaagatgccg tagacgcatt ggttcaatta aaacaggctg gtgtgaaaaa
    37561 cacagtcatg ttgacgggcg actcgaaaaa aattgctgat catatcggga agcaagtagg
    37621 cgtggacaag atatatagcg aattgcttcc agaagataaa gtgcagcggt tagaggaaat
    37681 cttacaaagt aacaataaaa agaccgcatt tgtcggagat ggaatcaacg atgcaccggt
    37741 gctagcaaga gcagatgtag gaatcgcaat gggtggattg ggaagcgatg ctgcaattga
    37801 agcagcagat gtagtcatca tgaatgatca accatcgaag attgcagaag caatccattt
    37861 agcaaagaaa acattgaaaa tcgtcaaaca aaacattgtc tttgctattg ggataaaaat
    37921 tttggtactt tccttaggtg cttttggttt tgcatcaatg ggagatgcag tatttgcaga
    37981 tgttggggtg acagtattag ctgttttgaa tgccatgcga agcttgcatg taaaaaataa
    38041 acgttgatca atgaagagga cgaatgaacg tcctcttttt tttatatcct tttcttaatg
    38101 aaaaatgtaa agaaaataga taggattata aaataactaa tgtttttaat tttgcagaaa
    38161 attcaaaata gcttgaaatt caataaggtc aatgaaataa aagaaaataa cttatttttt
    38221 tgatggaaaa aatatgaaaa aacgatgtgt attaaataaa ataatacaca cataaaataa
    38281 cactgtcttg taagtttgtt aaaaattgaa tatgattaca tctggataac aaatttttat
    38341 cataaaggag agaacgaatg aacaacaaac aattacgctg gtttactgtt gcgttaattg
    38401 catttaacat ggtttggggc atggggaatg tagttaataa ctatgctcag caaggagtat
    38461 cagttgtcac ctcttggcta ttgattttgg ccatttattt tattccatac gcattgatcg
    38521 ttggacaatt aggatcagcc ttcaaggata gtaaaggagg cgtcagttca tgggtcgaaa
    38581 atacgacatc taataaacga ctggcatact atgccgcttg gacttactgg gtcgtccata
    38641 ttccttattt ggcacaaaag cctcaagcaa ttctgattgc tggtggttgg gcagtccaag
    38701 gaaatggaaa tatcgtaaat acgatgtcag tacaaatggt agcagttatt tcacttatta
    38761 tatttcttgc ctttttatat ttatcaacaa aaggactttc tactttgaaa gtgattggcg
    38821 gactggccgg aacagcgatg ttcgtcatgt cgatcttatt tattctttta gctgtaacgg
    38881 caccgttcat caatccaacg atggaatttg ccacaccaca tatgaatcaa gtgaaaacat
    38941 atattccaac atttgatttc agttatttca caaccgtttc gatgctagta tttgcagttg
    39001 gaggggcgga aaaaatatcc ccttatgtca actctacaag aaatcctgca aaggaatttc
    39061 ctaaagggat gatcttctta gcagcaatgg tagggatttg tgctgtatta ggctcagtcg
    39121 caatgggtat gctgtttgct agcgataata tccctaaaga cttgatggca aatggggcat
    39181 acagtgcttt tcaaatttta ggtaattact atggtatcgg taactcattg atgatcattt
    39241 acgcattgac aaacatgatt ggacaaatct ccgcattagc cttttcaatt gatgcaccac
    39301 taaaaatctt gttagcagat gcggatccag aatttgtacc aaactggcta cgtaaacgta
    39361 atcgtaaagg tacattggtg aatggttatc ttctgacagg tatactagta ggaatcatta
    39421 ttttgttgcc gatctttggt atcaaagata tgaatgaatt agtaaaatgg ttgacgaact
    39481 tgaactcagt tgtcatgcca atgcgttacc tatgggtgtt cttggcatat atgatgttga
    39541 ataaagctta taagaaattc aattctgaat ataaatttgt taaaaatcca aaaatcggtt
    39601 ttatctttgg tgcttggtgc tttgccttca ctgcgtttgc atgtatccta ggaatggtgc
    39661 caaaagtaga ttatactgct gatcctaaag catggtggtt ccaattattc acgaatatca
    39721 tcacaccaat tgtcttgatc ttattaggta tgatcttgcc agcaattgct cgtcgagaca
    39781 aaaacaaaga aattactgaa ccagaaacag tggttcagcc ttaaaataga aatacttgtt
    39841 acaagaggtt gtgacagaag ctacttctgg aacaccgtga atcagaggtt gtgaaaaagc
    39901 tgtcctgcat caagtaataa gaacagccaa acaaaaatag ctcctcatat tttgccgttt
    39961 tttgagctaa tgctgaaggt cagctgattg aacaccattt attcgaaaca agggactttg
    40021 acacgacttt tgtcacagtc ccttgttttt ttgcggcgtt ataagtggta tgatcgaacc
    40081 aaattccaaa gagaagtgag ggagaagaat gaagatcatc gtgattggac aaagtgggtc
    40141 gggaaaatcg tccctcgcaa ggaagatcaa agaaatcacc ggctttccgc attcaggcaa
    40201 aaaagtggtt tttgcaagaa cagcaatcgt ttatagtatc taatccgtca tggatcgttg
    40261 aaggaaatta tacttctacg ttagaggaaa ggattaaaga agcagataag atcatttggc
    40321 ttaaagtgcc gcgttatctc gccatctacc gagtagtttg gcgttctcta aaaagaaaga
    40381 taaacaaaaa atctcggcca gatatgcctg aatcattttc ggagaaacta aacaggaaat
    40441 acttagaatt tttatctttt atctggcatt ttgaaggaaa caatgaacca aaaatacagc
    40501 agctgatcca agtagctaat gcaagaaata aactgatcat ccttaaaaca agacgagaga
    40561 aacaagaatt tttaactaaa ttgaaaatag agagcaaaaa taattgacag tttttgttta
    40621 caagcgtaat ctgtattcat aagctaatga ttacatttgt aaacgaaagg tgtgaaataa
    40681 atggaaaaag tcaaaacgcc agtaaagatc agtgattcag agtgggagat catgcgtgtt
    40741 atttggacac ttggacagac gacagcccaa gaaatcacgc agatcttagc tgattcgatg
    40801 gattggaaac cagcaacgat caaaactttg ctgggacgtt tggtaaaaaa agaagtgatc
    40861 tggacagaac aaaaaggaaa aaaattcatt tatcatccag ctgtctcgga gatggaaaca
    40921 gttcgcagtg cgacggaaaa tcttttttcc catatctgtg caaaaaggat tggagagacg
    40981 attgcggatc tagtagaaga agctactttg actcaagaag atatcaatga gattttgaaa
    41041 caactggaac aaaagcaacc agtagatacg atcgaatgta actgtatccc aggtcaatgt
    41101 gaatgtaaac aacatcaata ggaggaagaa aaaatgaaac aacaattttc aatcgaaggt
    41161 atgagctgca atcattgtgc agcaagagta gaagaaacag taagcgcact tgacggcgtg
    41221 caaaaagtga aagtaaattt gaaaaaagca aacggtacgg tcaagtttga tgaaacaaaa
    41281 gttcaatccg aaaagatttg tcaagcaatc aatggattgg gttataaagc agaggtgatt
    41341 tgatggcaac agcagaaacc aaaatggata cgttcgtcat tacaggtatg acttgtgcaa
    41401 attgttctgc taggatcgaa aaagagctga acggacaacc tggtgtggtc aatgccacag
    41461 tcaacttggc cactgaaaaa gcaagtgtca aatatgaagg gacgaccact gaaaaattaa
    41521 tccaaagcgt agaaaacatc ggttatggtg caattttgta cgatgaagcc cacaaacaaa
    41581 aaattgcaga agaaaagcaa gcgtatttga aaaaaatgct attcgatctt atattgagta
    41641 cggttttgac tttgccgttg atgttgtcaa tgattgctat gatgttagga agccatgcgg
    41701 cgattgttca cttttttcat tttccaatcg tccagttagt gctgtctgct cctgtccaat
    41761 tttatgtcgg tgctcgtttt tataaaggtg cgtaccatgc aatcaagacg aaagcaccaa
    41821 atatggatgt attggtagca attggaacat cggctgcctt tgcattgagc atttacaacg
    41881 gatttttccg tggacacccc caagatcttt attttgaaag cagcagtatg atcatcacgc
    41941 tgattttgct cggcaaatat ttggaacata cagcaaaaac gaaaacaggt aatgcaatca
    42001 aacaattgat gtctcttcaa acaaaaaccg cccaagtgat aagaaacggg aaagaagaaa
    42061 cgttagcgat tgaagaagta gtcgttggtg atcaactagt catccgtcct ggtgagcaga
    42121 ttccagcaga tggtcgaatt atttcaggaa gcagtgcgat agatgaaagt atgcttactg
    42181 gtgaaaatct tcctgtagaa aagaatccgg atgacacgct ttttggcggg acaatcaata
    42241 cgaatggtct gctacacatg gaagtcacac aagtcggcaa gcagacagta ttagcgcaaa
    42301 tcattcaaat ggtggaagat gctcaaggga gcaaagcccc aatccaaaaa atcgcagatc
    42361 ggatctcagg gatttttgtt ccgatcgtat tagtcatcgc gtttatcacg ctcatagcta
    42421 caggtttgat cactggtgat tggcaactcg cattgatcca tagtgtctct gtattagtca
    42481 ttgcgtgtcc atgtgcatta ggtttagcga ccccaacagc tattatggta ggaacgggtg
    42541 ttggtgcacg aaatggaatt ttaattaaag gcggagaagc attagaagca gctgctcact
    42601 tagacagcat tgttttggat aaaactggaa cgattacaga aggaaagccc aaagttacag
    42661 atctagtcgg ttcaaaagaa gtcctatcta ttttttatac tttagaacaa gcttctgaac
    42721 atccactagg taaagcaatc gtggaatacg gaaaactgca agaagcagca acatacgata
    42781 tgattgattt tacagcccac ccaggtgctg ggatcagcgg tacgattaat ggagtacgct
    42841 attttgcggg tacacgtaaa cgattaattg aattgaacct atcatttgat gaataccaag
    42901 aacacgcgtt agaattagaa cagcagggaa aaacagtcat gtttttagct gatgaaaagc
    42961 aagtgatcgg gtggattgcc gtggctgatc aaatcaaact agaagccaaa caagcaatca
    43021 agcagctcca gaataaagga ttagatgttt ttatgctgac aggagacaac aaactggcag
    43081 ccgaaacgat tggtaaacaa gttggtattg atccaaagca tatctttgct gaagtacttc
    43141 cagaggataa agcagcctac gtagaaaaac ttcagaaaga tggcaaaaaa gtcggtatgg
    43201 caggcgatgg aatcaatgat gcaccggcct tagcattagc tgatgtcggt atggcaatgg
    43261 gaagtggaac ggacattgcg atggaaacag ccgatgtgac gctgatgaat agcagtttgg
    43321 catccattgc acaaacaatc gagttgtcta ggttgacatt gagaaaaatc aaacaaaatc
    43381 ttttctgggc ttttgtctat aatacgattg gtattccttt tgccgcacta ggctttttga
    43441 atccaatcat tgcaggagga gcgatggcgt ttagttccgt cagtgtattg ttgaactccc
    43501 tgagcttaaa tcgacacatg aaaaagtaag agaagggatg gagtaaaaat gaatgggaac
    43561 gatatggaga aaaaacatga gcataaaaaa gaagtagtaa acaaagaaag tacagaatct
    43621 catgaaaaac agatggagat gaaacatgac catagtcaga tggatcattc catgcacatg
    43681 ggacataacc atggagatat agcaaacagc aaacaaatgg accattcgat gcacatggat
    43741 cacgaacacg gcggcatgga tcattccatg cacatgggga atttcaaaca aaaattttgg
    43801 ctatcgctta tcctagctat cccaatcatt cttttttcac caatgatggg aatggaattt
    43861 ccatttcaag taacgtttcc tggttctgat tggctcgtat tgatcttagc aactatacta
    43921 tttatctatg gcggtcagcc atttttaagt ggagctaaga tggaattgaa acaaaaaagt
    43981 cctgctatga tgacactgat cgcgatgggg attacagttg catatattta tagtgtctat
    44041 tcgtttatcg caaatctgct caatccacat acacacgtca tggatttctt ctgggaattg
    44101 gctactttga tcgttattat gctactaggt cactggattg aaatgaatgc tgtgtcgaat
    44161 gccagcaatg ctttacaaaa actagcagaa ttactgccag aatcagtgaa acgcttgacg
    44221 aaagatggaa aagaagagac agtttcattg aaagaagtaa acgaaggtga tcgtttgatc
    44281 gtccggtctg gtgataaaat gccaacagat ggtgtaatct tgaaaggtga aacaattgtt
    44341 gacgaatcag ctgtgactgg cgaatcaaga ggaatcaaaa aacaagccaa tgacaaagtg
    44401 attggcggtt ctatcaatgg ggatggaacg atcgaaatcg aagtaactgg aactggtgaa
    44461 aatggttatc tagcgaaggt tatggaaatg gtgcgaaaag cacaaggaga aaaatccaag
    44521 ctagagtcat tatctgacaa agtggcaaaa tggctgtttt atgtcgcatt aattgtgggt
    44581 atcctggcat tcatcgcctg gttgttcttg acagatcttc ctaatgcatt agaacgtatg
    44641 gtaacagtct ttatcatcgc ctgtcctcat gctctaggtt tagctatccc attagttgtt
    44701 gctcgctcca catcaatagc agctaaaaac ggattacttt tgaaaaaccg caatgcactg
    44761 gagcaagcca atgatctaga tgtgatcatg ctggacaaaa caggaacact aacagaagga
    44821 aaatttactg taacgggtgt cgaagtactt gatgatgcgt tcaataaaaa tgaaatcctt
    44881 caatatctag gagccttgga agcgaatgcc aatcatccat tggctgttgg cattatgaat
    44941 tatttgaaag aacatgaaat caagccttat caagctgaaa atctaaaaaa tctttcagga
    45001 gtcgggctag aggcaacagt caaaaaacaa caagtgaaaa tagttaatga aaaagaagtt
    45061 gagcgtctcg gattaaacgt agaacaagca ttattgaaac cttaccaaga acaaggaaat
    45121 accatcagct tcctgatttt agaaaatcat ctagctgcaa tcgttgcttt aggtgacgta
    45181 gtaaaaacag aagcaaaaga atttatccga acattgaaag aaagaaaaat cactcctgtc
    45241 atgcttactg gtgacaataa aaacgctgcc caagcagtag cagattattt agggatcgaa
    45301 gaatattatg gcggtttatt accagatgat aaagaagctg ttgtccagaa ataccttgac
    45361 caagggaaaa aagtaatcat ggtgggtgat gggatcaatg atgcgccaag tttagcccgt
    45421 gcttcaatcg ggatggcaat cggtgcaggg acagatatcg caatcgattc tgcggatgtc
    45481 gtattgacaa acagcgatcc aaaagatatt ttgcattttc ttgatctagc caagcaaacg
    45541 cgaaagaaaa tgatccagaa cctctggtgg ggggcaggtt acaatattct tgcaattccg
    45601 ctagcagccg gaattcttgc gccaattggt atcatattaa gtccagcagt tggtgctgtc
    45661 ttgatgtcgt taagtacggt agttgtagct ctaaatgctt tgacattgaa aatcaaataa
    45721 taatgaaaaa aggttgtgac aaaaaacgtg tcacaacctt tttttcgagt aatcgatact
    45781 caatcagctg gttctcggca ttccagaagt agattctcca gaaattttat cttatttttt
    45841 gaagcggctc ccttttgtca gaattttttt gtataccgtt tttcttatta tacgcgttat
    45901 ttctaaatag gtttttttat cgaattttac cggatctgct tgtcgtccat atatttctgc
    45961 ttcatattga gtagttaatc gaatgaaacg cagatctagc ttgaattctt gttctattcg
    46021 caggctatat tcctgcaaag attctgcggg tttgcgcggt accaatgttt ccaaagggtt
    46081 aagaagtttt tgatatgcaa tcgtattagt agaagcaaaa aaatgaagat agataagata
    46141 acttgtccag cgcagagatt ttctgaataa caatagaaag aacaacaaga cggataaaca
    46201 cactccaagg ctgaataaga cgattttata agctggtgtg agtgttgttt cttctgatgg
    46261 aatgacctca gatctctcac tggaaatagt ttctgccata ttttcgcttt catttggttg
    46321 ttcgatagca ggctggacgg tttcggttgt ttcagcaact ggttgtgaac ccattggatt
    46381 ggcaaatgaa ggggttggtt caaatggaag ccagccaaat ccaggaaagt atacctctgg
    46441 ccaagaatga gcatgactat tgagaatgct gtattcctca ttcccttgat tatctgtgcc
    46501 tgttctgagc ccatttgtaa aaccttttgt ccagcgagct gggataccaa gtgttcgaag
    46561 aaggacgacc atagaagttg aaaagttgtc acaatagccg acttttgaat caaagaggaa
    46621 ataatccaca taatcgtgtt ctttcggagt atgcccggta tccgttttgg aatagcggaa
    46681 atttccgctt gtttttaaat actgttagac agcttcgact ttacgaatta aagttgcttc
    46741 atttttggtc aattcgaccg ccaagtcctc aatacgttta ggcaaacttt ttggcaattg
    46801 aagatttgtc tgctgatttt ctaaatcatc agcttttaac tgactgcttt ttaattcttc
    46861 tctcgtaaaa actaaccttt ctgtttgtaa agttgcagaa cgtgtcgatt cattcgggac
    46921 aggaatgaat cgctggcttt caggcaaata gtaaagcgtt tgtccactag ctaatgaaaa
    46981 atcagtcaag gtagtatttc cataaggata aggtaagaaa gcttgttttt ttagggtcaa
    47041 tgtaatgttt tctttgtctt ctataacggt ttggtagctc gtgatgtcaa cagggaattc
    47101 ttggattggt tggatgggtg cgtcctgatc ttctatccat ccagaaccgg tgtaatgatt
    47161 tttattttcg atcttccaat agccatttcc tttttggttc gctgtaaaaa caacttcgcc
    47221 attatcatat aaaggtccac caagctgttc atcattctca ctgaagcctg ttcttgcact
    47281 tccggttaat tgatactcat ggagtgtgtc ataaagccct tcatcattaa gatattctcg
    47341 gatcggttca ctcgtttcct cgagagattg gtgtactttc ggaagatata aaggcaagat
    47401 agcgctaaaa gttaagaaac aaataagcag aactgtagtc aatgactgga atcgagtgtt
    47461 tcccgacaac aaccggatgg agaaaaattg ccttgctgcc agatacacga acaaaacagt
    47521 tactaccgca atcatttcgt acagtaaatc ttttttccga aaaacggtaa gcatcatgaa
    47581 gtaagccatc aaagaaagaa agggtgcgta ccaatagtcg taatccacgg tcaatgtaat
    47641 aaataaatag atcagcaaca ttatgagaaa aaaagctgtc ttttctggca aataataaag
    47701 tttaccttgg gatagatgga agaattcctc tgtaactgac gaaaaaaact gtaagatcca
    47761 taatatggaa aatgtcttcg aaggaaaata taaaaacaaa caacctatca ttactggcaa
    47821 agaaagcagc aaacgtaccc attttttagg aatacagcag acaataaggc ataggacacc
    47881 aatagttagg ataagaaggt agctgttttg caattgatta gctactacaa atatcggagc
    47941 acctacactt agtatcacaa aggagtaaac gccacgcagc catttttccc gtttaatcgt
    48001 caatccattc acctcctggc aatgaacata tcttgtcttt ttcttggacc atgagttgtt
    48061 tttctacgaa ataaacaaga taagccgttc gatcttttga caatgtttca atagcttctg
    48121 agacttcagg agaattctca ggaacaaaga ggacgatttg tttttttgca tattttttca
    48181 agtgtgcgaa aaaagctttc tcatcgccag cttccaaaga agcaaaatag gtatgaccgg
    48241 ggtctttgcc atgaaaggcg cggttgccta atatcagaag ttccggctgc ttttcttttc
    48301 tcaaatgcca taaagaaaaa tacaagtcta gcagaggttc aaattttgga ctgggcgaac
    48361 cccaaaagca aaaaagagga gaacattctt cttcttgata atcgtattca cggtataacc
    48421 actcttgagt atgagcagac aatttccaat cgattcggtt gaaagaatca cctggacgat
    48481 aagcgcgatg ttcccgaaaa tcaagacctc tttggtacgc gttcagctgc tgtttttttt
    48541 ccagaactct ctgtaatttt tctgcttgaa tcttacaaaa agcaggcaga acagtcacag
    48601 gaaaagagat ctctcttttt gcactttttc tgaaaagtcc tagactatca taggctgtaa
    48661 aaatgacagt taatgtttca tggcatcctc tttcttttgg ctgccaaagc aattggactt
    48721 cttgcggttt atttgtaaga atcaaaatct cttgtttttt tctgcaaaga ttgctgggaa
    48781 gccgatttgt aaacgaggga gaggtaaaga aaaagttctc ttttttgcta tggaaaaaat
    48841 aacctgcata ctttttttgc gataaacgag tggctgcaat gtacaagtaa ccgaaagttt
    48901 ctttagaaag ctgcttaatg aaatcaagct ccatgataca taaagagtaa aaaaggccaa
    48961 caagaaccag cctaaagaat tgttgaatac aagagcaaaa agcacaatga tgaaatatac
    49021 cagaaataac acaaaattaa tgaaataatc tttgattttt tccattgtct acctcaatct
    49081 acctcacagg aataggaatt tgtgcaacaa gagtatgaat gatttgtttc ttttcgtctt
    49141 ccttcaatga acgatcgtaa aaaatcaggc gatgggaaaa cacgtaagga actaaatcct
    49201 gcaaatcttt gggcgtgacg taatttctcc catggattaa agcataagct tttgctgcct
    49261 gaataaagga aaggcttcct cgtggactga tgccaagctg gatggcagca tgacttcttg
    49321 ttgctgacac taactgcaag gcataagatg ccacttcggg actgacaaag acattcgcca
    49381 catttgtttt tatttcttct atttcataac tgttcaatac ttcatttaag ttatccaaca
    49441 ctttttcgaa tttatccaaa agtagcaaaa gttcgtcatc aaaagaagga taaccgattt
    49501 gtagtcggaa taaaaaacga tcgagctgtg cttccggcaa aggatatgtt ccttcgtact
    49561 cgattggatt ttgcgtagct aagacaaaaa aatgattgtc tagagggtat gtattgttgt
    49621 cgatcgttgc atgattctcg gccattgctt ctaaaagtgc tgactgagta cgaggagtcg
    49681 tacgattgat ctcatctgcc aacaagatcg tggtaaagat cggtcccgga cgaaattcaa
    49741 actcttttga ttgtgaattg tatacggaaa aaccaagaat atcactaggc agcagatcag
    49801 gagtacattg gactctgctg aaggttccct gtacagcttt tgctagtgct ttgaccagta
    49861 acgttttacc tacaccggga atatcttcga ataagacatg accacctgct aatagggcca
    49921 cgatcatcaa ttggatagtt gaacgtttgc cgatgaccgc tttttctaat tcttatatca
    49981 actgatcgat tttttgtttg gattcttcta ttttttcttc cattttcaca tcaccttctc
    50041 ataaaatcgc tatttatttc attgtaatgg aagggaatac atcctacaag caaagtgatc
    50101 ttattatgtt tttttaaagt acattcgttt tgaaatgaga atacgattaa attgagtaaa
    50161 ataaaaaata atgaggaaga tttgcgtatt taaataaata gaaagggagt tttttatctt
    50221 tttaggagtg aactcatttt gatcaaacaa tatgtttaga aatcaggtga gaaatgatcg
    50281 atgagatggc aaaaactatt atcaaaaaat gcacagaatt tattttcact aatcaatcta
    50341 atgaaagatt tgatctggcg aaaagctgat ttagctgaag aactgaaaat cgatgttaaa
    50401 acagtggatc ggcatctgaa acgtttggcg gaaagcgact gtcccattca gctgattctt
    50461 cataagaaaa ccgttcagtt agtttatgat caaggatata gcgaacctta tctcttgttt
    50521 gtttttctga tccaatcacc cccgttttgt ttactgaaaa atctgctatt aaatgaacct
    50581 tacgagcaga agtacgatcg ttctacgcaa gagcgattga gaaagtggtt tggcgagtac
    50641 cacctttctt tttcttataa aaaagcacag atctttggtt cagaaagaaa agtaagatat
    50701 ctgctgtttt actttttgaa agattttccg catttttttg cagatgatgg agagcagtcc
    50761 ttttctgaga tgttcatcca attattgaag caaagaaagg atccggcaga aatcgagatt
    50821 ttaggagaca gtcgaacact attctttgaa ttgtttcctg aattgctatt tagagaggag
    50881 cagattttgc caataaaaga aaaaaattat ctagcaattt tgcgcttgag ctgtaaccag
    50941 attactgaaa agcggtggaa acggatagaa gagacacaag actatcggaa attgacgatt
    51001 gtgactgaaa agcttgattc gctgttttgg ctgaatagcg aatttgagag cgaaagcaaa
    51061 tcaaagctga taaaagtact ttaccaagaa tggcttcgtt acgtaattgg tatccatgac
    51121 cggctgatag aagaagttcg ttattatcag gaaacattag aaaaatttcc caattccaaa
    51181 gataaactat atcaatttcg gctagcgatc caacaagcac ttgcacaccc cgcagtagag
    51241 tgggggatgc ttttactgaa aacactccag gaacgagggt atttagatgt ccaccctcct
    51301 gaagtgaaaa tcgtcttttt ttttcgctat gaacaccaag aagcaagaag acttgctggg
    51361 gtactgaaca aaacgctaag acatcgaaaa cggatctcta tacatgtatg tactcgaaat
    51421 aagccgcctc ggtatgcaga tctagtgatc accgatcatt tttttccact agaccataac
    51481 gaagatcaaa aaacgtatct gtaccatcct tcatttggta tcgaaagact tcttaccgat
    51541 atcaattact ggttaggcaa aaaccgataa aacctttgga aaaaagatca aaacaagtca
    51601 gtctgtgcta taataaaaag cgattagtaa aggaggaaat ccattggaat taaagaaatt
    51661 agaagttccc acatcttcga tcacagaagt gaaacgatcg ccgatggatg tatttgctca
    51721 agcgcgagac gcgggaactg gcgtatatat atttaatcgt gaaaaagtag ctggcgtgat
    51781 gctgacccaa gaacaataca aaacgctctt gcaagaacta gatgtccttc gtcaaacagt
    51841 gaaagtggag aggaaagaac caaaagaaat aaacgaagaa cctattaaca aaacagcttc
    51901 cgtcgtatcc aatgctgaag ggatggaaga gctggcccaa acattacagc actcgctcat
    51961 tactggtgtg acgattacag caaaaaatct agatgaacga atggtagcac taggttttat
    52021 tacgaaaaag accggatttg gcggagtcgt ggatatgctt agcgagttga tggaaactgg
    52081 gaaaatcaac taccagttaa gaagaaaacc gcaaaatcgg acaatcatag ctgaaatcat
    52141 aggagagcaa aacagtcaat ctcaattgat ggataaaatc atcatcaaga aaatttttct
    52201 acatgagcta taagaaaacc atgttcgcac ttctaggcaa gccaggcttt gaagaccttg
    52261 ccaaagaatt gaacgaacgc ctttaagata agggtttttc gctagattta aaaaatcagt
    52321 gatagaaaca tgttcgctta taaataaaat tagtgatagt tttagtgata gtgagcttaa
    52381 aaaagcactc aatttgagtg cttttttatt tgctcttcta tatagttttc tagttgattc
    52441 attgatcttt ttttcacttc gggagaaata tgggcataga cttgtgtagt ggaaacatct
    52501 ttatgaccta aaaaatcttt gatatcttct aaaggtattc ctgcttgtct cgttctagct
    52561 gaaaaagcaa aacataatgt tggtgttttc ctttcagcat atctatcagc aagatgtaga
    52621 gtaggacaac ctcgtgagcc aaaagtcctg tgcaaatcga gttacaacac acacatgata
    52681 aagaaacttt tgaaaagaac tataaagtat acgttgaatt ggcaagagat tcagcaatga
    52741 aatatggtat tccattaaca ttagacactc cttataacca accaggaatc aaatcgcatt
    52801 tatgggtaac acaaaatatt tggggcgatc atacagatcc ttacggttat ctttctgaaa
    52861 tgggtgtaag taaagaaaaa ctagcctatg atttggctca tggttttacg gatgataatc
    52921 caactacttc ggaggataaa ccagtaattg atccaactcg agcaggtgct gcaaattcta
    52981 cgctgacaga tggaacaaat tacgcccaca ttgatcagtt cggagaaatc gaaaacgcaa
    53041 acttgcatgt agctggatgg cacattgcta attataaata cgagtatatt ttcattatgg
    53101 actacaatac tggaaaagaa ctagctagag taagagctga tgggatttat agaccagacg
    53161 taaatcaagc ttataatact ttaggaaatg ttggctatca tgtatctttc aatatgcgtg
    53221 atttccctaa taagaaagta tacgtcatga tgcgtgcgac aaacgatcca gaaggaaata
    53281 ctaagggtgg agcacaagat tttcatgaca agcgttggta tttaaatatt ccacaacgat
    53341 aaaaaatccc cccctcttt
//
LOCUS       DAZBTF010000012        52300 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000012 DAZBTF010000000
VERSION     DAZBTF010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.012"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1412
                     /locus_tag="ACHGNS_000774"
     CDS             <1..1412
                     /locus_tag="ACHGNS_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFN6936741.1"
                     /translation="PTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTN
                     TPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNV
                     TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNA
                     DKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTY
                     PDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYT
                     WKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTG
                     KTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNV
                     VVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGL
                     ILLGTLGLLGYKNRKKENE"
     gene            1546..2889
                     /locus_tag="ACHGNS_000775"
     CDS             1546..2889
                     /locus_tag="ACHGNS_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFN6936742.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            3001..4452
                     /locus_tag="ACHGNS_000776"
     CDS             3001..4452
                     /locus_tag="ACHGNS_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFN6936743.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            4801..5433
                     /locus_tag="ACHGNS_000777"
     CDS             4801..5433
                     /locus_tag="ACHGNS_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFN6936744.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            6021..7460
                     /locus_tag="ACHGNS_000778"
     CDS             6021..7460
                     /locus_tag="ACHGNS_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN6936745.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            7755..7973
                     /locus_tag="ACHGNS_000779"
                     /pseudo
     CDS             7755..7973
                     /locus_tag="ACHGNS_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            8222..8437
                     /locus_tag="ACHGNS_000780"
     CDS             8222..8437
                     /locus_tag="ACHGNS_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936746.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(8654..8910)
                     /locus_tag="ACHGNS_000781"
                     /pseudo
     CDS             complement(8654..8910)
                     /locus_tag="ACHGNS_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(9097..10008)
                     /locus_tag="ACHGNS_000782"
     CDS             complement(9097..10008)
                     /locus_tag="ACHGNS_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFN6936747.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(10027..11031)
                     /locus_tag="ACHGNS_000783"
     CDS             complement(10027..11031)
                     /locus_tag="ACHGNS_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFN6936748.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(11028..13151)
                     /locus_tag="ACHGNS_000784"
     CDS             complement(11028..13151)
                     /locus_tag="ACHGNS_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFN6936749.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(13156..15603)
                     /locus_tag="ACHGNS_000785"
     CDS             complement(13156..15603)
                     /locus_tag="ACHGNS_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN6936750.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(15590..15979)
                     /locus_tag="ACHGNS_000786"
     CDS             complement(15590..15979)
                     /locus_tag="ACHGNS_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFN6936751.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(16039..16542)
                     /locus_tag="ACHGNS_000787"
     CDS             complement(16039..16542)
                     /locus_tag="ACHGNS_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFN6936752.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(16555..16779)
                     /locus_tag="ACHGNS_000788"
     CDS             complement(16555..16779)
                     /locus_tag="ACHGNS_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936753.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(16883..18793)
                     /gene="ltrA"
                     /locus_tag="ACHGNS_000789"
     CDS             complement(16883..18793)
                     /gene="ltrA"
                     /locus_tag="ACHGNS_000789"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFN6936754.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(19539..19676)
                     /locus_tag="ACHGNS_000790"
     CDS             complement(19539..19676)
                     /locus_tag="ACHGNS_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFN6936755.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(19673..20857)
                     /gene="mobT"
                     /locus_tag="ACHGNS_000791"
     CDS             complement(19673..20857)
                     /gene="mobT"
                     /locus_tag="ACHGNS_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFN6936756.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(21116..21406)
                     /locus_tag="ACHGNS_000792"
     CDS             complement(21116..21406)
                     /locus_tag="ACHGNS_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936757.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(21396..22538)
                     /gene="dcm"
                     /locus_tag="ACHGNS_000793"
                     /pseudo
     CDS             complement(21396..22538)
                     /gene="dcm"
                     /locus_tag="ACHGNS_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(22614..22778)
                     /locus_tag="ACHGNS_000794"
     CDS             complement(22614..22778)
                     /locus_tag="ACHGNS_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936758.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            complement(22771..23445)
                     /locus_tag="ACHGNS_000795"
     CDS             complement(22771..23445)
                     /locus_tag="ACHGNS_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFN6936759.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            complement(23523..24863)
                     /locus_tag="ACHGNS_000796"
     CDS             complement(23523..24863)
                     /locus_tag="ACHGNS_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFN6936760.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            complement(24946..25281)
                     /locus_tag="ACHGNS_000797"
     CDS             complement(24946..25281)
                     /locus_tag="ACHGNS_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFN6936761.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(25291..25665)
                     /locus_tag="ACHGNS_000798"
     CDS             complement(25291..25665)
                     /locus_tag="ACHGNS_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFN6936762.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(25678..25992)
                     /locus_tag="ACHGNS_000799"
     CDS             complement(25678..25992)
                     /locus_tag="ACHGNS_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFN6936763.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(26105..29332)
                     /gene="fss3"
                     /locus_tag="ACHGNS_000800"
     CDS             complement(26105..29332)
                     /gene="fss3"
                     /locus_tag="ACHGNS_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFN6936764.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            30189..30443
                     /locus_tag="ACHGNS_000801"
     CDS             30189..30443
                     /locus_tag="ACHGNS_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936765.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
     gene            30607..31524
                     /locus_tag="ACHGNS_000802"
     CDS             30607..31524
                     /locus_tag="ACHGNS_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFN6936766.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            31517..32299
                     /locus_tag="ACHGNS_000803"
     CDS             31517..32299
                     /locus_tag="ACHGNS_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFN6936767.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            32300..32980
                     /locus_tag="ACHGNS_000804"
     CDS             32300..32980
                     /locus_tag="ACHGNS_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN6936768.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            32973..33869
                     /locus_tag="ACHGNS_000805"
     CDS             32973..33869
                     /locus_tag="ACHGNS_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN6936769.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            34081..36003
                     /locus_tag="ACHGNS_000806"
     CDS             34081..36003
                     /locus_tag="ACHGNS_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFN6936770.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            36004..36450
                     /locus_tag="ACHGNS_000807"
     CDS             36004..36450
                     /locus_tag="ACHGNS_000807"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFN6936771.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            36466..36780
                     /locus_tag="ACHGNS_000808"
     CDS             36466..36780
                     /locus_tag="ACHGNS_000808"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFN6936772.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            36806..37855
                     /locus_tag="ACHGNS_000809"
     CDS             36806..37855
                     /locus_tag="ACHGNS_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFN6936773.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            37867..40470
                     /locus_tag="ACHGNS_000810"
     CDS             37867..40470
                     /locus_tag="ACHGNS_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFN6936774.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            40587..42236
                     /locus_tag="ACHGNS_000811"
     CDS             40587..42236
                     /locus_tag="ACHGNS_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFN6936775.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            42552..43487
                     /locus_tag="ACHGNS_000812"
     CDS             42552..43487
                     /locus_tag="ACHGNS_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6936776.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            43480..44250
                     /locus_tag="ACHGNS_000813"
     CDS             43480..44250
                     /locus_tag="ACHGNS_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6936777.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            44665..45648
                     /locus_tag="ACHGNS_000814"
     CDS             44665..45648
                     /locus_tag="ACHGNS_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFN6936778.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            45969..47006
                     /locus_tag="ACHGNS_000815"
     CDS             45969..47006
                     /locus_tag="ACHGNS_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFN6936779.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(47095..47418)
                     /locus_tag="ACHGNS_000816"
     CDS             complement(47095..47418)
                     /locus_tag="ACHGNS_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFN6936780.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(47405..48112)
                     /locus_tag="ACHGNS_000817"
     CDS             complement(47405..48112)
                     /locus_tag="ACHGNS_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFN6936781.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            complement(48411..49835)
                     /locus_tag="ACHGNS_000818"
     CDS             complement(48411..49835)
                     /locus_tag="ACHGNS_000818"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN6936782.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            50010..50723
                     /locus_tag="ACHGNS_000819"
     CDS             50010..50723
                     /locus_tag="ACHGNS_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN6936783.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            50872..52203
                     /locus_tag="ACHGNS_000820"
     CDS             50872..52203
                     /locus_tag="ACHGNS_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFN6936784.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
BASE COUNT        16416 a         9692 c         9851 g        16341 t
ORIGIN      
        1 aaccaacagt agaaggcgaa aaagtagaag ttgggggcac agtagatcta acagataatg
       61 ttactaactt gccagcgtta ccagaaggaa caacagtaac agatgttact cccggcggta
      121 caattgatac caacacacca ggtaattacg aaggtgtcat tgaagtaacg tatccagacg
      181 gtacgaaaga tacggtgaaa gttccagtag aagtgacaga caatcgttct gacgccgata
      241 aatatgaacc aacagtagaa ggcgaaaaag tagaaattgg aggcacagta gatttaacag
      301 ataatgttac taacttgcca gcgttaccag aaggaacaac agtaacagat gttactcccg
      361 gcggtacaat tgataccaac acaccaggca attatgaagg tgtcattgaa gtaacgtatc
      421 cagacggtac gaaagatacg gtgaaagttc cagtagaagt gacagacaat cgttctaatg
      481 cagataaata caatcctgaa ggacaaaaag tgactacaga cttaaataaa gagccagatg
      541 catctgaggg aattaagaac aaagaagatt taccaaaagg aactacttat gcttggaaag
      601 agaaagtaga tgttagcaca gctggaaata aaaaaggtac tattgtagtg acatatccag
      661 atggatcatc tgatgaagtt gaagtagacg ttacagtgac agatactcgt tccgatgcag
      721 ataaatataa tcctgaagga caaaaagtga ctacagactt aaataaagag ccagatgcat
      781 ctgagggaat taagaacaaa gaagatctac caaaaggaac tacttatact tggaaagaaa
      841 aagtagatgt tagtacagct ggaaacaaaa aaggtactgt agtagtaaca tatccagatg
      901 gatctaaaga agaagtagaa gttactattt ctgtagtaga taaaaaagca ccgaataaac
      961 ctcaagttga tcctattaca gagggtgacc aaattgttac tggtaaaact gaaccaaatg
     1021 cagaggtgac agttacatta ccagacggaa gtcaacacca tgggacagct gataaaaatg
     1081 gtaattttac agttaaagtt cctaaattag aggcagggac aaaagtgata gtaactgcaa
     1141 ctgatgaatc tggtaatact agtgaaccta ctaatgtagt tgtttcttcc aacaaaaaag
     1201 acagtggaaa aaacggtagt aaaggtagta agactgacaa tcaaggtagc aacagcaatc
     1261 aagacaaaaa tagaggcaaa agccagtcct caaaagtttt tcctaagacg ggagagagtg
     1321 acagtaatat ctttactatt agcggtggtt taatactgtt aggaacgtta ggattattag
     1381 ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa tttcttaagt cttctctgag
     1441 aagaggaaga atctttttct tcctcttttt gcttttttat gatgggaaat aagtgttagt
     1501 taaagatgaa tatacttttt atagaaagga aaagaatgta attaagtgga aaaaatagta
     1561 attattggtt caaaccattc agggctagcc gcagctcgat ttctaaactt aagcgattca
     1621 aattatgaag ttattctaat cgataaaaaa tcagaaatta gttacttgga atgtgggaca
     1681 tcgttattta taaaaggaga agtaagtaat ttagaagatc ttttttatca aaatagaatg
     1741 acactaaatt ctgagataaa aaagttattt ctagagacag aagttgacaa aattgatatt
     1801 caaaataaaa agattagttg tgtaaaaaaa gatggtacaa gattctctca agaatatgac
     1861 aaagttatat tagctacagg ttcaaagcaa attatgctag atataccggg gaataattta
     1921 gctaatattt tttcgataaa gaattttcca aatgcaaaag agatttttaa agctttggat
     1981 gatagtacta ttaaaaaagt aggaattatt ggagcaggat atattggagt tgaaattgct
     2041 gaagccataa gaaaaagagg gaaagaagta tatcttttcg atgttgcaga tagagtgttg
     2101 tcgacttatt acgaccgtag tttttcagat aaagttgaag aaatactatc aaaaaatgga
     2161 atacatcttt gtctatcaga aagtcctaaa aaatatttag gaagaaaaaa gattgaaaaa
     2221 atagtcacta caagtggtac ccaatactct atggatatgg tcgtacaagc tataggattt
     2281 ctatctaata gtccaattgg ggaaaaggaa ctcttgagag atgttagtgg agcatatcta
     2341 actaatgaat accagcaaac aaatataaaa gatatatatg ctattgggga ttgtgctaca
     2401 acttttttta cgtccattaa tagaagtagt gttgattttt ctatttctaa tgctctaaga
     2461 acagcttata ttgcaactca acatattaac aatcaagatt ttacaccttc tggttcccaa
     2521 tttacaaatg gcttttctat atttaattac aatttttacg cagcaggttt gaatttaaaa
     2581 acagcacgat tattaagtga gtctatagat tatatagaaa tagaagattt cattagacct
     2641 gcttttttga gaaaaaatag tcgtgtaaaa ttaagaatta gctttgaaaa aaatacaaag
     2701 agaatagtag gtgtacaact ttgtagtcaa gaagatttat ctggactatt atcaatgttt
     2761 tctcttgcta tagaagaaca aattacatta gacaaattga aaatgttaga ttatttattt
     2821 catcctagtt ttagccaacc ctataatttt attttaaagg cagtaacagg attaaacatt
     2881 gaaacataat cgttactaaa tgaaatcaag aaatatactg tattactcaa ttcattgaaa
     2941 atatttatca taatttttaa tagaaaacac tattaaagag aaaaaagaga ggtaaatcaa
     3001 atgaaaaaat ctttaaaact tgcatcagca gttatattaa tgtcccaact ccttatagca
     3061 acacctttcc atgtgttggc aagcgaaaat tcaacataca aaattgcaaa agaaagaacc
     3121 gagacgactg aaatgagtaa ttccgatgta acagcagaga gtagtgaact tttgacaact
     3181 tcatcaagtg aaagtagtac gacttcaaat gccgagtctt caacaacttc atcaagtgaa
     3241 agtagtacga cttcaaatgc cgagtcttca acaacttcat caagtgaaag tagtacgact
     3301 tcaaatgccg agtcttcaac aacttcgtca agtgaaagta gtacgatttc aagtaccgaa
     3361 tcttcaacaa cttcaagtag tacaagtgaa ataacttctt cgaaagtgac aaaaccaaac
     3421 catatagaat cttcaaaaaa ggaagcgcca aaaacggaag gacaaacaaa aactttttcg
     3481 acaccacagc aaatagaatc aacagaatct ttccaatcac aaccaatact atctggtaat
     3541 gaagaaattc attttgacaa aaatgaatct gtagaaaatt ttattatgaa aattggtgag
     3601 tctgcgagaa aaattggtca gaaatatgat ttatatgctt ctgttatgat tgcacaagcg
     3661 atcttagaat ctgccagtgg tcaaagccag ttagctcagg caccgaatta caatttattt
     3721 gggataaaag gaacttataa tggaaacttt gtcattatgg taacaaacga agatttgggt
     3781 aatggaacgc tttatacgac ccaatcaaaa tttagagtgt atgaaaacta tgaggaaagc
     3841 tttgaagatt atgccaaatt attaactaag ggaattagtg gaaataaaga tttttatgca
     3901 ggagcattaa aagctaatag taaaacatat cgagaagcaa ctaaattcct tacaggacgg
     3961 tatgcaacag atacacaata ctatttgaag ctaaatgaat taattaaaac ctacgattta
     4021 accaactatg ataaagaagt taaaggtcca caaatgagta agactggata ccaagtgcct
     4081 attcataatt attcaataac cagtttgttt ggaaatcgtg gaaatgaatt tcaccgagga
     4141 cttgatatgg cagctacaca gggtgagccg attcatgcag ttaaagctgg taaggttgtt
     4201 aaagcagagt ttcactactc ttggggaaat tacgtggtga tacaacacga agatggatca
     4261 accgcgttat atgctcacca gcaacaatac ctagtaaaaa ctggagataa tgttttgcaa
     4321 ggacaaataa ttggctatgt aggaagtact gggaatagta ctggcccaca ccttcatttg
     4381 gaaatttgca aagatagtag tctttctcag agcatgttag ttgatccgaa aaccttatta
     4441 ttcggagact aaaaaaggta atcaatctga caaagtaaca agaaaatggt tggattctta
     4501 gacctttgat caggatattg agactggaat tgatatctcg attacgatgg gtttgacaaa
     4561 taaaacaagt ccattatcga atatcgagtg tattattgcc gtctttatgc tcatattttg
     4621 agcaggtttc actagatgaa aacaatttat ctatcttgag gatttcgtgt acgtatcgtt
     4681 cagtcttgta ttgtaaatta gtcataaaat cagactaaga aaaattggaa atattttttt
     4741 ctgatttatt aaaagttaat tgaaattata tggtacttct ttcttgggag ggtaggtgca
     4801 gtgaaggaaa gatatggtca ccttactgtt gttgaggaga ttactcataa tggatataaa
     4861 aaatatcgtt gcctatgtga ctgtgggaat gaaacgattg tatatcgatc aaacttacta
     4921 tcaggtagaa ccaaaagttg tggatgtgga aattataaaa ataggcataa atataaaaat
     4981 ttgacaaata aaacttttgg taagttagtt gcaatcaagc caactaaact gcgaagtgaa
     5041 ggaactgttg tttgggaatg tcaatgtctg tgtgggagaa aaaagttagt atcggcaaag
     5101 cgacttctaa aaggtgatgt aaaaagctgt ggatgtttga aatcaaaaaa gcatcacatt
     5161 gaaaataaaa aattcggatt acttactgca aagtatcctt tggacaataa ctgttcatgg
     5221 aaaacaaaat gggtttgtca ttgtgagtgt ggcagtatgt gtgagataag ttatagtaac
     5281 ttattttttg gacatactca aagttgtgga tgtttaaaaa ggcaagaaac aaggacatgt
     5341 gttgaaggga cagtaataga gtgtctaacg tcaaaattac cgaaaaataa tacaagtggt
     5401 gttaagggag tttatcgttc taaagagaaa tagattgcgt atataacttt aaaaaaaaag
     5461 aggtactatt taggatcgta tgattctatt aagagtgcag ctcaagctag aaaagatgct
     5521 gaaaataaat tgtttcaacc tttgattgac aaatatcgag gtaatcatta attattgtgg
     5581 ataataatta acagtgatat ataaacatct ataatatatt attcatacta ttgatattta
     5641 gtataatcct atggaattga atatcatagc aatgtatttg aaaagcaaat tggaaaacaa
     5701 ataggtgcaa caccataaat atgaaaaaat tgggaaaaag tagaatggaa atttgcttac
     5761 gtctcacgag atagctcaca ctattaatct gaagaacaat gaagtcgact tttagaaatt
     5821 gctagaagaa agtaaagcga attatcacta gttactatag agtaagttca aataaagcat
     5881 ctccaactta catggttaca tgaggatatc aaatcgaaat tactcaagct tcctatggct
     5941 agagtcgcga gaagttgttg ggcagtagac ataggttata ttgttcttag ctacacgatc
     6001 attagaaagg aaatcctatt atgtatagtt atcaagaaga tgcaaaagtc caaaaaaatc
     6061 gctggtggat tttgattgct atatctatgt ttaccttcat gtccacattg gatggcagta
     6121 ttgtcaatat tgcactacct accatctcag atgatatggg cataccaatg aatcaagcgg
     6181 aatgggtagt gtccgtttat ctgatgattg tctgttcttg tttgttgctg tttggtaaaa
     6241 ttggcgatag ccgtgggaaa atcaaagtat ttcggattgg tacgctgatc tttgtcttgg
     6301 gatcgctgtt gtgtgggttc aatcattcgc taggtatttt attgttcgca cgggtggtac
     6361 aagccattgg tgctagtatg actatggcta caaatgccgg aatcatcacg gaagtctttc
     6421 ccatacatga acgtggtcgg gcattgggag caataggtgc gtttgtttca ttaggctcga
     6481 ttgctggacc aggtcttggc ggcttgattt tgtcccaatt ttcttgggcg tacatttttt
     6541 ggatcaatgt accggtggga atcgtaacga tgttgattgc tgaaaaattc ttaccaaaag
     6601 atatcaccat gagcggccat acaatcgatg gagggggctt tgctgccatt gcggctacga
     6661 ttatcagctt ttttggagca atctttttgg ggcaagaaag tggctttttc catccagtcc
     6721 cccttgtact gttcgtaatt tcagtgattg cctttgtcag ctttttaaaa gttgaaaaga
     6781 aagcagcctc tccattgatt tctttcggta tttttaagaa caagattttt accatgagct
     6841 tgattacagc cttgttgatt ttttcttcca acttctttgt caatgtagtg attccttttt
     6901 atctgcaaaa tgctcgcggg ttgagtccta gctatgctgg gctgttgatg atggtctttc
     6961 ctttgatgat ggtcgtcggg gcgcctttaa gcggctacct gaccgaccgg gttggcccgg
     7021 agattttggt attagtaggc ttgggcttat tgtcggtaac ccaggtcatg tatatgtttt
     7081 tgtcagtgga ctctcctttg tggctataca ttgtcgctac tgccatcatg ggtgccggca
     7141 atgccttgtt ccagtccccc aataatacga tggttatgag ctcggtcact atcgaaaact
     7201 tgggactggc aggctcgatg aattcctttg cccgaaattt ggggatggtc agcgggattt
     7261 cgctttctac gacggtctta tatcaagcga tgtctgctaa gatgggccag caagtaacta
     7321 actatatcgc aaatcggccg gatgtctttt tatatggaat gaagattacc tttttaggca
     7381 gctttgtttt gtgttttgtt gcctttttcc tgactatctg gcgtatcaaa aaacaaggag
     7441 gtattcagaa aaatgtctga aaaaaccact gttgaagaat ttttggaggc ctgcgtatgg
     7501 gtgagtggga tttccatgga tgatggaaga tgaagtgaac ttctaaaagc cgaaaaactc
     7561 aagtattatc ttcaaaatac tagtgctcaa tgagatgctt gatttgatca caagaagact
     7621 gcgaaagccg ctggcaaccg caagctaggt atcccaccga tggactacgt tttagaggct
     7681 actggttatg aatacggagc caacgctctc atctgcataa gaatacatca cccagattat
     7741 gtaaagattt tcgatacttc tgtcaaaaaa tcattaagat agataaatga tttccgtcta
     7801 gtcaaattgg ttcagaatgt agacacacag atggtaagaa gtcactcaca attcgagaat
     7861 ggacttgtcc tatttgccgt atacatcata atcgaaacgt caacgatagt atcaatattt
     7921 tgaccaaggg ctatgaatac aagcattgcc ttagaaaaca tcaagaacct taggaacaac
     7981 ggggatagct tggttaataa gagataccgc tgtttaggct tgcaagtata ctcttttatc
     8041 aagaattccg tggctttagt catgagaggc ttaatactat gaaaattttc tttagcattt
     8101 ttgatttagg ttccttttct aattataata agatgaccac tacagtaacc tactgaaata
     8161 tataaacagc cactgtagta ccattttcaa agtagtctat gagaggagaa tcttcattct
     8221 tatgaacgct ggaattgcgg atatgaattt aataaaaaaa acccttaatg attttacttc
     8281 caattcaata tcaaaaggta ctggtatcaa tttaagcaca ataaaaaagc tgaaaagtgg
     8341 tgaaagatca gtggaaaagc tgaatctttt agatgctata aaaataacgg aatttgctat
     8401 gaaaaatgga aaagctgaga ttgaaatatg gagataatta tcgtctagtg aattacttgc
     8461 cacttagctg aatttaacga tccttggtgc ttgaaggggg agattcttgg gaatagagca
     8521 tacttgcaag cctatgtctt tactaacaac gatatctctt attaaccaag ctatccccgt
     8581 agttctaagg cttttacttt tatctaagca ataatcgtat tcaaaggaaa tttttgaccc
     8641 ttagaaagtc tcattttatc agacgtgagc ttaggcattg ctgaattttg ttaattcggt
     8701 tttaggttgt gctttgagca taatatgaaa gtattcttta tcacgatttc attcatctaa
     8761 ggtaatgtga tgtgattctg aaattctttc aaaggcagtt tttgcataag ctgacattac
     8821 atcatcaatg acatgtttac aatgtttcac tactaaaata agggggtaac caagaagaaa
     8881 aactgaatga ttatttgtat ctaattccat ggtatatcaa ccatttccta tatagactga
     8941 ttatgacatg gattaaaata aaaaggcact atgtcttaat tgtcggtttt cgagttaccg
     9001 aatggcattc atcacccacc tatagaggat gggcgacttc tgcctgaaat aagttacact
     9061 acttataact tctaataaaa ttagattgag tcattattat ttaacatcaa cgattttcca
     9121 gtttccattt ttttgtaata ctaatttgta ctgaaagtag ctagtggttt ttgatacatt
     9181 atcaaggtat tttatctttg tactcactag aaaatttttt ccgttttgta gataaactgg
     9241 attctctaac tccgaaaact ttaagtcaat attgattggt cgcatcgcac cattttcaac
     9301 ataatactcc aattcttttt cagaagctga tggataaagt ttaaaaaatg tttctaaaaa
     9361 atcggtgatt tcttccgttg ttttggcatc aatttctgaa tcgttttcta cttgttttgc
     9421 ttcatatttt gccttgtctg gctttccaat cattgttggc aaactagtta ctaaatgatt
     9481 cctgttttca tcttcaaaga ttgtcactcg atatgcagaa gaaattgatt gcgtcttttt
     9541 tccctctgta atatgttgct ccactaaaaa tgaaacgaca aattcttccg agtgtttttc
     9601 aacatctaga atttttacag attgaacttc ggaactcgtg ggaatatctg ctcgaaccat
     9661 atcttggatt aaagcgagac cgttttctgt aaggtactgt tcgagattac tcattctctt
     9721 ttcaattgct tctttgttat ttttccatga aaaatattct ttcgcaaaat cctctgtaaa
     9781 actttcaacg ccagaagtat tcactacctt ctcttttata accacttttt catgagttgt
     9841 atgttgatca atggcggtga aatttttata aacgccaaaa gcaagactac aagacaataa
     9901 aagccaaagg gcaagcacca tttttcgatg cttacccacg gatacaactc gttctttttt
     9961 cttttttggg ctcttttcct tttgattccg ttcaattttt atcttcattc tttttcctct
    10021 tttctcttat ttgtatcggc cagctccgat aagatgctgc cgccaatagt tttccgttag
    10081 attcgtccac cccactggat ctcctgcatg aaacatgcga ttctcccctg cgtaaatgcc
    10141 cacatgggtc acataagtac cggcatcata ggttgaatga aagaaaacta aatcacctgg
    10201 tttggcatct tttagatcaa tatgagtcat ggcatcatat tgatcctgag ccacccgagg
    10261 aagtttcagg tcaactttgg cataacacca ttgggtcaag ccactacaat caaaccccgt
    10321 ttctggactt gagccaccaa acacataggc cgttccttca tacttaaatg cctcatcaaa
    10381 aatttcttgg actattttgt catcaaactg agttgtagtt aggtattgct tcactaataa
    10441 acagtaaaac atattgccat aattataccg ccacccacca ttttccttaa tcgcgattgg
    10501 gttggggtaa tcgacttttt gaccacctga tttttccttt gaaaaagatt cagctaaagc
    10561 aaaactatgc ttcttcccgt ttttcccgac atgatctaga tacccgccac catagttata
    10621 ggcctgaata atactatctt gatcacaccc tagttctttg gaacgcttaa ccaaagacgc
    10681 aaaatagaga cagccttgtt tgatggatgc ctcggtactt aatgaattag gtggtaatcc
    10741 ggctgactca gaactttgca taacgtcttc cattgtgcca ccagattcca cttgaataat
    10801 cgccaaaaga attggaatat actctgttat atccgattct ttggcatact tttccaccat
    10861 tgcacggtgt ttcaatactt cttctgagac actagttccg ccaggagaag tgccagaact
    10921 accatttccc tcactgtctt caccaaacaa caaacccaca cacatcaaaa tacccataaa
    10981 agagatggtc agaaaagtta atgaaataac acccactttc ttcaagttca ttttttctca
    11041 cctgaccttg attttggacg atggttcatt cttttttgta ctttttgatt ggtctgcttt
    11101 ggcagagtgg acctctgttt cacttgttgg aaagaatttt tcttacttgc tgattggctt
    11161 ttcccttttg aaatgatttc ttgactagtt agttttcgtt tgatttcggg attcagattt
    11221 gctctcacaa tccgctttgg tgcttctttt ttcagcatca tatctttctg aggttgagct
    11281 gctttatcta ttggaatatt cgcccttctt tttccctgac gatttgttgc ttcactcagt
    11341 atcttgcgtt tttcgtccat tttcgcattt cttcgttgtc gttgtttttc tcgcaaatcc
    11401 actcgatccg ctttttcttg gaacagtccg cgcttgaatt cttctggtgc ttttttcgac
    11461 ttttcgattc ctttatggag attgtatttc aaattcgttg gcgtattcct tacctgatcc
    11521 tttacttgtt tagtcttatc tttcaccaaa gcttgggtat ccaaaacttt gccgattttt
    11581 ctcccagcca aattcattcg ggattgtttc ttattatttg aaactggatt ctccttagtt
    11641 aagtcagata gtatttcgtg atcgttcggt aatcgtgaat tctttcgtag agaagaaccg
    11701 gatggcttaa atttggattt tgattgttcc tttgccaact tatttccgac tgctgcacca
    11761 ccaccaaaag ccaaagctcg tcccacattt cgattgagtt gccgcaattt tcgattcatt
    11821 aacatttgcg gttttcgcat gacgcgtctt cccattgact ggctatcatt actctggagg
    11881 ttgaacatac tcataatgtc gcccaatttg aaatagatac cggcaaaggt cacaatttgc
    11941 agaaaagcaa ccatgaaaaa tggataggtt gccgaaatat tgaaaaacat cgtggagatg
    12001 ctaaatgcag tcgtaatcac caaagtaaca cccgctcgca tcataatcgt attgaataaa
    12061 cgaataatcg ccttctttcc taaactttca taagtcggca acatggacaa taagaaacta
    12121 attggcagaa acatcgcaaa gataatgaag agaatttgcg agaacaacat aatccctgtt
    12181 agaagaaaaa caaagaagga aatgccaata ttgaacaata ccaaaaagac cacggtccct
    12241 aaacggttca tggtcttggt aatactgaga ttcgcattgt cattgtcttc aatctctgcc
    12301 ttgaccgctt cttcccgatc tttgcctttg ttttcatctg gactcacaga tagaatcttg
    12361 ttgacccggt cttcaccaat ttcttctatg ttggaatcat caaattgcag tagtaaccaa
    12421 ggttgttgga cttgaatcga aaacaaacta tcccgaatca aatccacact atcttttcct
    12481 tggctttctg aattgggtac aacgatctcg gttccaaggg taagggccgc ttcactaaca
    12541 tctgagctaa agtcattgat ttttgtgata taggtaggcg cataagcaat gaacgaaccc
    12601 gatagtagaa aaatcatcac aaaattaatc acggctcgaa ctgctttcgt ggtttctcgt
    12661 tttaataatc cggtatacgc cacatacacc cccagagcta aaatcatcaa taatagaaac
    12721 ccaaaataaa atccggaggt ctggagtcca ttttcagtaa taccagccaa tgtttggata
    12781 ttctttccaa tactttcagc cgtatccgaa atgaaatcta atttgtaagc ctcttgaacc
    12841 acataacccg tggcgttcga caaatacaaa ctcaccgtcc aaatgaaatt agtaatggca
    12901 taaagaccat acatcacgct tttccctaag ccatcacccc aattccaagg caaccaatcc
    12961 caagagctat ccacaaagaa atctagttga tagttgttca gtgaatattt ggaatataga
    13021 tttcctgctt caactgtatc atcaactagc cctgtcgcct cggctaccgt accaactaaa
    13081 ctcaatagaa ctatcagagt gagtatgcta ataaaaacga ttcccacaat ccgaaaaagt
    13141 ttctttttca tcccctcact ccattctctc ttgaacaggt ggacgggtat caaaggcatg
    13201 gaagagttct tcaaagactg gatgtacctg aaccacgcct acccgaccat acaaatcctg
    13261 catcaagcat tggccatttt ccaaatctcg taggcgtttt tgattgcttt catcttctcg
    13321 atccaagcca aagaattcca aggttttctt gatttcatga atatcggtac tgcgaaacgc
    13381 aaacttcaac ccgatattat tcttcaagcg ttcatccagt aaatcatcac tattttgcgt
    13441 gacaaagtaa accccggcat tcatagcccg cccagcacga acgagcttat tcgataatgt
    13501 tttcccttgc gctacttgaa gaaaactcca cgcctcatct aagtctacaa ttttgaagat
    13561 ttctcgatta ctatggataa agtccaaggc aaaagtggaa atgacgatta gcatggaaac
    13621 actaagcaat tccatggtgg tatattcttc aaaagtggtt tctgcatctg gtaaaaccaa
    13681 atccgctact tgaatgatat ttaactgcct atctaaacta atcgactggg taatcgaacc
    13741 atctgagaaa agcaaatggg caaaatcata atcggtaaag gactcgatgt gatccgcgat
    13801 attttccgca actggtgagc cttcttttcg aagctcatta attacttgta ataatccccg
    13861 ttgattgctt tgggtaaccg tccgaatggc cttacgcaac actggaaact tctccccatc
    13921 tcgactagaa attcctgtta aaaaggtcag aatatcaatt gctagacttt cagaatcctt
    13981 ctttttctcc ataatcacat aaggatcaag taatccttga ttttttggct tactagttaa
    14041 gttgagaata ttgatttcat ccgcaatttc tggcaaggtt tctttccagc caccccgttc
    14101 cccttttgga tcgacaatca cagcttgtcc accaaaaagg acggcgtaat aaactaagag
    14161 attgttacta aaggactttc cgccaccgag tgaaccgagg aatgccgctg ctagcgcatt
    14221 ggtaactgaa tccttgaccc cttgtgaggc caatgccggt ttcaggtaaa cattgcgccc
    14281 cgtatctacg ttataaccaa agtaaatgcc ttccaactcc cctaacatct gagtggcgcc
    14341 aaaaccaagt ccggcgagaa aatcagacgt aacatactgg atataatcat tcatgtatcg
    14401 cttactagaa ggtaagaatt cttcatgtag ccccatcata tcgccaaaag gccggactag
    14461 ttttacattg gtatcatcat aaaaatctag cacttcatca caccggcgct ttagttcatc
    14521 tactgattcg gcacttactc gaaccacata gctcagtttg tacatggatt ctttggactg
    14581 atccaaggta gtttccaatt catctacgga atccaatgca tctaagacat tggaatttgt
    14641 ttcattgtca gattgatagg catggttgtc caagtctttt aattcctttt tcttattgcg
    14701 aacggtagca agtgcctttt tgttcgtcac aatctccaca ttcatactag tgtcaattgg
    14761 gaatgtaaat tgttgttgct ggtaatagaa aagttctgag gagggaaact ccatttcccc
    14821 cacaattgta ttaattgtta agtacgctac atatgattca tgattctcat gttccatgca
    14881 taagtaccgg ggcttttctt caatcaaaca acgactggga cgtaataagt cgtaacgttt
    14941 caccaaagtt tccgacttca gctttttctt tggtagctga aattcatatt cttcaaatgg
    15001 tgtacccttt tcgccataga tatgttcaat caaatacatc agatcactcg gtgtgactcg
    15061 tctgacttta aatcttcgag ccaatttact ttccattagt ttttcaagct tggtatatcg
    15121 gcgaatttct tcattggata aggaaacaaa atcgcccatc aaatgatgat tcactccata
    15181 gacaaattct tgaaagccgg agaagaaaga tttcttgaga ttctttagat tgacctcttc
    15241 atctgtcgca atcagtttga agccaataaa aaagcgataa tcaatctgac tatcgccaat
    15301 catggatact aatgcctctg tctgtaaatc aattcgttgt ttcgctactt ctgttaatcg
    15361 cccggtaatt tccttttttg atcgttcttg cgtggcacga atactacttt ctgtggcaat
    15421 ttgtaaggca tgaatcttgc cttcacgatt ttgcgcaatc aattgacgaa agttatcgtg
    15481 gacttgatat ttctgttcag gtgacaaaaa ggagtaattg taaggtatta actcatagta
    15541 ggcaaaacat tctccctctt gattaaagac aagattattt tcaatatact taatcgggta
    15601 catagttcac actccttacc agagtaatgg gttcgtccca tcgtttcttc tgttctttga
    15661 ctttctttcc tgcgaaagtg accttaaatc gtaagtgata gctaataaaa gatcgtaaaa
    15721 atcccaatgg tttcttaccg tcaaacgttt tttgcgacat gaaccaagta actgccaccg
    15781 gtatacctaa gtattttaaa aaggccccct ctatcataga tagtggaggc acatgggcaa
    15841 atacgatgac aaacagcaag gataaaacaa accaagccat ctggttaaaa gtcacaggaa
    15901 aaggcagttg aaagtcatta attgcataaa tgaccttttc aactgcccaa atactggtgt
    15961 agctttttat tttcttcatc aaatcattcc tttaaaaaga aggatagttt agaaatcaac
    16021 tacccttcaa taattttgtt aatatggata ctcaaaaaca cctcgactcg ttaccaaata
    16081 attgcctcca atctccaagt ctctaccaca cgcatcaaaa tcaatatacg tcttcaagtg
    16141 ttctggtact tctccaaaat caccagcttc tacaatcaag aaatatgcta catcactcat
    16201 atcatcgcaa tctggataat agataatatc atccacatgc tctaccatct cctcaaaaga
    16261 attgaagaaa gcatgttgaa tctcagaagc aacttcacca actggcgttc cttcaagttc
    16321 ctccgctaag ccacacaacc gattaatttc ctcaattggc gtatactcat caatctcaaa
    16381 gggaagttca taatcatgaa ttgcatattc ctcgtattcg tcatttaacc caattcgttc
    16441 tttcacttca tcaaaatcga caggtggggt aaaccacgcc cccaccaatt caccttcgtt
    16501 atatttcccg aggttcgcaa tataaacacg catctgttcc atcgtgcttc ccccttacgc
    16561 accgacaatt cggttaaaca aactgagtaa gacatccttc accccagaag cattaaagac
    16621 caatccgacc gcaatcaaag caatcaccaa aaagccaatc agtttggaaa actcccgctt
    16681 aaatcctaaa aacaaaccaa tgaccacaat tgccagtaag actaaggatt gggcattggt
    16741 taaaaaccat tgatatatgg ataggtaagt tcctgatatt ataaatatct caaacttttc
    16801 cccccattca cacggagcgt gcgactttca ccgcactccg cgttccatcg tgtttatatt
    16861 gaaaagtatt tatgtagtag acctatatca agtttaattc ttgattgcca gctaatactc
    16921 tatatttatt tagtcttatg agctggactt tatttagctt caatagagct atatattttt
    16981 gtattgtttc ttctgtttta gcgtgtatta gacggtgtac cgcctctttt accaaaataa
    17041 gatttttata atcgtcattt ccaccagctc cagttggtac aatatgatga caatgtattt
    17101 cattagcttg ttgaaattct tccagagtta tagcacattt tccatgttga gcagaaaata
    17161 gcgaaattcg attgtctgcg tattctattg tgcgattttc caaagataat ttcattaact
    17221 ggtacatcag taatttatca acacttagat tggtgtggat aaattttctt ccctctagag
    17281 tgtagcaatt cgctaaatac acctttgcca ttggttttct gtgttggata tatccaacag
    17341 ggaaaatagg ttcgccctcg ccagcaacat accgaagcat tttagattta ccatatcttg
    17401 ttgcctcctt tgtcgtaagt tgtctgcctg tttttctcaa tctgcccctt ttgtttgtac
    17461 ctaatctgtt agtgaagata gccattacag ctctattcag tttcatacaa tccagattta
    17521 cattcgttgc aatctgataa taattttgca tacctaatac cattgaatta taaagtcttg
    17581 tttcttctaa ttccgttttc ccttttgagg gagaagcaat tttctttgcc tgtgatacca
    17641 gcttgtcttt ttggtttttg aattgtttat cacttatatg tgatttaaca actaacttct
    17701 ttcttctcgg tatcatttta atcttgaaac caagaaagtc ggagtatctt tttctaacat
    17761 ttacaattct tgttttttct tgtgatactt ccaattttaa tcgttgttca atccattgag
    17821 tgattgctat tttggctttt tcagcacttt ccttgtagcg gcagaaaact ctaaaatcat
    17881 ctgcataacg aaccatatac atttccttta actttgtttt cttcatttct ttgtaagcgt
    17941 tgcttttaca tgggctaccg cttttattga tatgaaccac atagtttttt gtaacaggat
    18001 tttcctgcca ttggctttct acccaatggt ctaactcatt aagaacaatg tttgctagta
    18061 gtggggatat tattccccct tgcggagttc ctttctctgg atatgttatt gtaccgttag
    18121 tcattttgat tggagctttt aatatccttt ttaaaatgaa tatcaagtgc ttgtcccgta
    18181 ttcccatagc ccatatctgt tttatcagct tggaatggtt cacgttgtca aagaaaccct
    18241 taatatcaaa ttcaatcaca taatgtagct ggctgatttg taggcggtta taggttgctt
    18301 ttattgcatt ttcaactgac ctattaggtc taaaaccata gctgttttca ctaaatttag
    18361 cttcgcagat tggttctaat acttgtttta tacattgttg tataagcctg tcccacatac
    18421 aaggaatacc tagaggtctg gttttaccat ttggttttgg tatttctttt cgcctcactg
    18481 gcttaggtcg atacccatgt ggactgcctg caacaatgta tcttactttt ttgacaactg
    18541 tttctgctgg caatttgcca atatctttaa ttattgtacc gtctgttcct gctgttttac
    18601 tgcctgcatt tgctttgata tttcgatatg ccagtaatat attttctctc gataggataa
    18661 tatccataag atgagtgaaa atatctccat tcttactttt ggcatataaa tcatcaaagg
    18721 tgttttgcat ttcgtaatat tcagcgtgtc ttaaatcttc tacacacaat gtttcatttt
    18781 tatttttagg cataaggctt cactccttta cttgaaattg agtgagcttt tttatcttac
    18841 tcgtaatcct tattgaaaca acataatact tttctaatat atacacgact tgagggcatt
    18901 cctccattct cattacaaga atttcttagg ttcgccctct gcttttcagc attagttcaa
    18961 ctgcttattg ttcttcgtca gtttattctc ttgatgagaa cccaatacct ttcctcgttc
    19021 cgataatctt atctatacat atatatcctt aggtcactag ctataagcct gctggcttga
    19081 taccgcctgt aacgttatat ggcatttcat aacctatatt cttactttgc cacaccagct
    19141 acgatttctc gtacctttgc atttctacaa agttcaaatt tagacccgta cattcgctgt
    19201 ttcgtcagtt ccttatagaa cattctaacc atagatattt tatagacccc cgacctgttg
    19261 gatacgctac ccacctctgg atagttttcg atagaagata tatgttcatc aactattacg
    19321 gtatctttca gccgtcttta ccgagcttca aacattctga gtttcctcat acaatgcttg
    19381 tcggaggctc agggcaggcg tttcagagcg ttacttcatc attccacctg tcagattatc
    19441 agttctccta ataaccaaac tttttcgttc ttttattagt gccttagctt ttcactaagg
    19501 aacgagtcgc acataaattt tgtccgaaat tcatcttgtc actttccttt cttttccatt
    19561 gctcgatccg ctgctgcact tgcattcatc aagcacatca cagtaacacc aagcatactg
    19621 ccaccaaata ccaaaaaaat atcttttaca attcccatga cgattcctct tttcatataa
    19681 tcaaattttc aattgccata ctttgttgct ccaaaatctt tttatgtcgg tctgataagc
    19741 gtgcattctg taccatatcc tggatatagg ttgtccgatt caatcgatcc aactccttag
    19801 ccattttcca cgtaggggcg acttgtcggc ctagccaatt caaggttcgt tcaatggtat
    19861 acggttttgg ctcggaagtt aattgaatct ctttccgatt ccgtccaata aaacgccccc
    19921 attgttcatt caagggccag ttggttcgcc ttttaccttc tactttatcc gcaaatcgca
    19981 agtaacgatt gataatggaa aacgtggtgc tctcaatgct acgacctttt aacaaatctt
    20041 gaatcgcatg aaaagcacgg tcatttttca gtcgaatctc aaaacgattt ttcgtctcag
    20101 tttggtctaa tggaatccct aacttgatat attgttcata gtctttttca tacaaacaga
    20161 aataaacctc actcttgaga ctgccgatat ataaggtatt gcccatgccg tccttttcat
    20221 cagcctttaa aagttccccg gaacgataac ttttaaaggt acgaaacaag gagatacatt
    20281 cttccttctg acattttttc gttagctcag gtatatccaa tagccccact cgatcattga
    20341 ttgccaagtc caagcgtttc atcacgccac cggcctttag acaatcctcg aaaaagtcgt
    20401 accagctacg cttttgagcg agtaaaaaag tttcaaattg tcggcaacct cgccccttta
    20461 attctaaaag cacaccttta tcttcttcat gagacaacat caccacaata tcacccatga
    20521 catactgttc ttgataggag taaaaggcat aatcttcatg gagcatatac tcaaatttca
    20581 aatggagaat ttcttgaata atttttcgtg catccgttgt gggaaagcga atccttacat
    20641 agtcaaacag taacgtcaaa ggcaaatcgg gattaaagct ttccagttgg tcaaaaattt
    20701 tcctcatggt ttttatagat gcggttacgc caccattttc tatctgattt aagtacggcc
    20761 gactaagtcc acaagccgta gccaatttat tttgtgaaac gccgtattcg ttgcgtcggt
    20821 cttttaatag ccgataatct aaattccgtt gtgccaagtt ctcacctcca aattcaaggt
    20881 gttaactttt tcaaatgagt taacacctat acaaattact tactatcttt gatacaacaa
    20941 gcattcttaa tggttgctta cttatttttt gtgtttttgt acccccctgt tagatacggg
    21001 ggtgagacat catggtcggc taacgccgtc catgaacagg cacagcacca cggaggattt
    21061 ctgcttcgcg aatcctccgc ggtcagcgcc taacagtcag ttgtgttggt ttaggttact
    21121 catctgtaat accatttact tccaactctg ctttcatttc taacaagtat tgacgaatat
    21181 ctggttcttt tgcttggtcc gcaatccgct caaattctgc aattacttga tttatttcaa
    21241 ttcgagcatc cgtctcctca ttcattaaat atggatcgcc agcgactttg acaccattct
    21301 tgcgaatatc aaattttaac cgtcgcaatt gttcttctaa ttctgggtct gactgaatct
    21361 ttccaatcac ccaataaatt tctccaatca actcatcata aaccatgtac atccaactct
    21421 tttaatcttc tggcaatatc aaaaatgacc ggaactgtta cagagtttcc ggcttgctta
    21481 tacaactgac tatctgaatt gacttgagaa tctcgatcaa aagcccaatc gggaaagccc
    21541 tgaagtcgcc aacactctct aggtgttagc ttccgaattt gatactgatc cgtgacgatc
    21601 gcttgttggt ctcctgttag taaggtatga acactctgtt ttcccagacg acctcggcga
    21661 cttgtggaat taggaaaagc cagattcaca ctgtcttctg gcttcacctc ttgatattct
    21721 gtttcatttt ttcgaatcat gatttttgta ccaccttgtt tcgttgacaa ggtgggggcg
    21781 gttccagtaa cttcataaac tcgattacct tgttcaaact ttcccggtaa tagtcctgct
    21841 acagagattc catcccttgc tggcagtgcg attttgattc cttcatcacc tgttaaggtt
    21901 ggcgctacgc catctgaacc atagacttgt ccacccattc ctcgattgga aggattgata
    21961 tttaccaaat ttctgatgtt ttgatcagat gttgaagacg gtccaatgat aggaaatact
    22021 tgttcggtac atgcgtctcg agaatgtccg ataaggaata tccgctcccg gctttgggga
    22081 atgtaggcct ttgagttaag cacctaccac tccacgtcat accccaatcc atccagcgtt
    22141 cggaggatcg tctcgaacgt agcccctcct tcgtgattga gcaatcctcg gacgttctca
    22201 aggaataaaa gcttaggtcg gagaagagtg gtgaaccgag cgatttcaaa gaataaagta
    22261 cctcgagtat cggaaaatct ttgccgcttt cccgcaattg aaaaagcttg gcacggaaat
    22321 ccgccgcaaa ggatgtccac tggtccgagg gattgaataa actcatctga tacacttgtg
    22381 atgtcatgca tttccacctc cttacttgta tcatgaattg ctttataact acgtctagca
    22441 aatttatcaa tttcacaaaa accgatacaa tgatggctcg cctgttccat acctaaacga
    22501 aagccaccaa taccggcgaa taaatctaaa aatctcatac attacctcat tcattgcaaa
    22561 gaaaaaagcc aagaacattt tgctcttggc tcatacatta ttaatttgtc ctactatttt
    22621 ttatttaatc caactaattt ccgttgtttt tcattggcct gatcggctac atatttaatc
    22681 accgttttga actcttttgc agtagcattt tccgataaat ccgcaatgct cttattgtat
    22741 ttctttacaa ttttcgttac ttgttctttc tcagtcatgt aattgaacct ccaaatcttt
    22801 cctactatac cctagtggta aaaataattc tagattatcc gtagcataat gaacttcgga
    22861 aatattattc gtataatctt ttattatgat atgaagcaat acatctggat gtttactctt
    22921 tacttttgca atgttctctc ttgatttgaa aaaagcgtta ataaatgcct ttttcggcac
    22981 atgacgtccc tctgcaattt cacgttcttg tgtaaatttc caagcgattt tagggtcttg
    23041 ataaacatag aaaatcgtca ctcgaaaatg ttttttcaac gcccgcgaga tatttttttc
    23101 agcacttaat attgcaaaag ttgcatccag aataaaagaa tagccatgat ctaagacata
    23161 tctaaatgcc tgctctacca accaagaaga tgctttttga aagagactag agttttttcc
    23221 attataatca ggaaactggg tgcgaaagta atccgcatca ataatcacat ggttatcata
    23281 attctcagct aatccaatgg caacctctga ttttccagcc ccaggactcc ctgccataaa
    23341 aactgcgtct cgtccaactt cgggggattt ccctttgata atataagaaa taaacttatc
    23401 cttatttgct tttgcatact ctaaataaga atcattactt tccatattaa ttatcccctt
    23461 tcttcctact atataaaagt attatacgtt acttgaagta taaagtcagt attgaagatc
    23521 actcaatcat tcatcttttc taatttacta attgaatcta agaaatcata cccttttggt
    23581 acaagtggcg tataaaactc actaatcaca ctgccacctg tatccacata acctcgacct
    23641 ttgatgtgct tcataaagaa gtttttttcg acctccccaa acatcatgga atatccgagt
    23701 tcactcatac gacccaaagc cacgcgaaag ttgaattgat cgcggattcc gtctcctaaa
    23761 tatttcgcat ccggcctttg gcaagccaaa atcagaaaga aaccagattg acgacccaac
    23821 atcacgattt gtttgagctt attcaaaatc accgcacttt ctttcgtcgt taacatctcc
    23881 atataagcca cgtattcatc aaagattaaa aagttgggtg gaagtccaag ataggcataa
    23941 ttctctcctg ttttgtagtt agacatttct ttcattgcct tgctacgagc catcatacgt
    24001 tcatagaaat cttccacaca agcagaaatt tcttcctttt gtgaataaac atgaggcatc
    24061 accgtaccta aatcagctaa atctgcattt ttgggatcga ggataaacaa ttctgcatct
    24121 gacttcaata gtgcttcgat aatggtgagg aggaaatagg tcttccctcc acccgtgcca
    24181 ccagcgatta acatatgggg taaagaatca taagcccata cttgattttt catcaatcgc
    24241 agagtcccat tttctgccac tacttcgtct atcccaattc gattggcgat catatcatac
    24301 agtaaagtat actccacata ggagtctttg agttcctttt ctactaactc acaatacagt
    24361 ccgctttcta acttcttttc caattttaaa agctgatctt ggtatttccc aagtgaaatt
    24421 tgaacgcgaa tagacagtaa tccatctttc attcgataac agattttggg gaagtaactg
    24481 atagtctctc tggttcggct actattcaaa tctttgaaaa agccttcact ttgagtttgt
    24541 ttcacttcat accaatggtt ctctagaatc atccgagcta gcttttgtcg atgccacaag
    24601 cttcgccaat gatcaagaaa aaagtgaata taaagcaata tcatagctcc tgaaaaagcg
    24661 agacttccaa gcagacatac taaatctttt atagaccaaa cgatttcaat agtagaaagt
    24721 gatagcttag aaatatcaaa ttgtctaaac tcttgaaaat aaaagacgct catccaaccg
    24781 atgaacagag cgagtagcca gccaagaaca aagtgataaa ccaagtgctg atctcctgcg
    24841 cgtatgcgat gtcctttgta cattttgatg gtttccacct ccaatcgaaa aagcccaata
    24901 ggatttcatt aggcaatttc aaaaagttat gctagtcatt tactgctacg aggaggggag
    24961 ttgtttcgtt tcaaccaatt gatttaaaaa gccttcaata gcatccggca tctccacacc
    25021 aatttcttcc aaaatggatt ctgcctgccg acataagttt tcatttactt cgacaactat
    25081 aaagaaagac tcttcttctc ctaaccagtc tagtacatca gagtcttctt cactgtcgct
    25141 attttctcca agtaaacctt gatgtataat cagaaacatt ttttcatcag aggttaatgc
    25201 tttaacgagt tgttcctcag tcagctcgcc ttttccatat tgatcgagac aatctaaaat
    25261 atgttcaaca ttaatttcca taatcttcac ctatttcttt ctttcatttt ggttcgtagg
    25321 atttccttga ttcggctgcc gcttcaagac taaatcatcc gccttaatga accaattaac
    25381 ggtagttcct tctcgaaaaa cataattggc aacggtatcc acgattggat tgaccaactc
    25441 tacctctgca tcataagcaa actcgcgtaa gggtacatta gctgggatac taacttgaat
    25501 cattcgccct tgtgcagagg attttaaatt ataggttcgt tcttttacct cattaccaat
    25561 tgttccatcc tcattttgct tccgtacttc tcggcgtaat gcggaaaaac gcaacttccc
    25621 aaatgttaaa cctgaatcta cgacaatacc ttctgttaat ctcatcttct tttcctccta
    25681 tgctttcact aaatcatcaa catacaacac gtaatcagta aacccacgat tctcaatctc
    25741 atagcctttg gcaaccaaat ggggattcac ggcttttagc ggttggtctt gatcaaaatc
    25801 cttttcaccg gcttccgcag ctactaccac ctcaatatca tcggcccgtt gcttactgga
    25861 atacaaatga taactgcgac caatcaccgt agtattccgt ccatatcctt ctgttataat
    25921 ttctccttca ccggcataac ttaactttcc aaaggtttca gccatatctg gcacgacaaa
    25981 tttcaattcc atcttctttt cctcttttca ttgttagttt tggatacaaa aatagcgcga
    26041 ctactttttt aggaggcggt cgcgcacgtt ggttcttagg atttacttgt atcatctcat
    26101 caccttagtt ccggcgattc cagaaataat agcccgccgt cgcaaaaatc aaagtaaaac
    26161 caatgaacag taaaacattg gaatttttct ccccggtttg agggaaggtc ttcactgtgg
    26221 gactagattc ttgagagcca cttggaacag ttggtgtttc cggttgtttc ggcgtactta
    26281 gtatggttgg aacttctttt ggtgttaagg tttgagattt ttctttcaaa tcttcattgt
    26341 gttttccatt gatattccca tctttatcgt aattgatttc cgcaaaagta aacttggttc
    26401 cttctggata ttttccggta tctacttttt tcgcaagcac agttttggtg aattctttat
    26461 catttacttc ataatcaatc ttgccagatt tccaaatttc tttgtttgta ccatccggta
    26521 ataaagcata cagaattgtt tcgaaagctt cttttgagcc atccagtaca tcatgagtaa
    26581 tcgacacatc atcaaacata tccaccacat caccatgagt aaaggtctga gaaccgtttt
    26641 ctaggtgggc ctttgtttgg atagaaacat ggtgttccac tgtacaactg acagtttggg
    26701 cttggttatt caagctaaca tccttcgcta ctggcttatc gcctgcttca aaagcttcct
    26761 tattttcata cacatagttg aataagacaa tagttttatt taaaacttgt tcggcggtta
    26821 atttatgctg aattttccaa gttcctgttt tctctttgtt cgccaacgtt gcgcttgttt
    26881 ctgcaatcac cactggtttg gctttttcat ctttttcttg ggcagctttt gtcgcatcca
    26941 catcaatggc ttgcgctacc acataccagt cttctttgat ttcatgtaga ttgtaactca
    27001 atttatcaac tagctcggta gaatccagag aagttaattc tttgtttccg tctttccaag
    27061 tcgcaagaga agttaaacta tcttcttcct caggcaaatc atagagcatt aaacggttca
    27121 aactaacaga aaatgctttg ttcgtcagct tctcgtaagg aacggtcacc gttttaattg
    27181 gttgtttttg gtcttgttcg gtaatggtaa agacatactc actcttcaca aagtcttctt
    27241 tgttttcctt aaaagtagaa cggatttcta atggcgtaat cttttgaaaa ccttctgggg
    27301 cttctacttc ttcaagtaaa taatccccat aaggaagatt ttcaaacttg ccataaccat
    27361 caaaccctaa ttgctcgtta taagctgtgg tcgcttcatc ttcggcacct gtgatttcat
    27421 tggtcccttc caatggcgac actttaaagg tcagatcatt gaagccagtt tcggtagtgc
    27481 ccgcagctga tccagcaaat ttaaagaaat caaagccaaa gcgaatcact tgttcttttg
    27541 ccgtaacatt tcgggtaatc acggcatttt tttcgctatc atccaccttt ttgatggata
    27601 caggatactt tgtttcatca aggctatatc cttcgggcgc ttttgtttct tgccaataat
    27661 actcgttgat agctaaatgt ttaacggcaa cttggttctt ttcgtctaaa gtcaaagtga
    27721 ccgtttcatc agaacctttc gttcccttcg ctaactctgg tttaaaagcc tcactccatt
    27781 taacagcttt tccatcctta gcagtaaaca aggtgtattc agcaccctca aagactgcct
    27841 taccttgtgc tttatcccca gtatctttgt cttctttcgt caaagtggtt tcgccagtga
    27901 cttcttgatt ttgccctttt acgttactgg taacaggggc tacggtttga ttggcatact
    27961 tcaattcgac ttttacaggt ttgaacgtat tcacgaaccc atgactagct ttagtttcgg
    28021 tcacataata ggttcccaat tccaacgcat tagcaatctc ttttggtgtt tccgcatgac
    28081 cgttttcatc cgtggtcatt tcttgaacga tttcaccggt tggactgtct ttgcgaatgg
    28141 caaacgtatt accagctaag gaataattgt cattccaaag atcactccct gtttcaactc
    28201 cgcttttatc cagaatgatt tgcccttttt cccgtgcgtt ttttgaagtc acgtaaatgg
    28261 tttcgcctgc tttaatggtg gtagtcatgg gggtggtatc aatcgtataa ggcgccggta
    28321 ctgatttttc ggtaatggtt acttttgtac catgaggaat ctcatccaat gtagcaatgc
    28381 cttctttgtc agtagtcacg tcttttgcag gtaaagtttt tccaaagtct aaatggaaaa
    28441 ccgttcctgg tacaacatca cccgattctt tatcgacttt tttgatcttt aaagaaccct
    28501 ccgtttccac atcaattttg attgtgtagg tattaggttt atcaatcgcc ccggccatta
    28561 aagtctgttg gccagccttt ttataggcga caggtgttcc ggtaccagca gatttcttaa
    28621 gcgtcagcac accactttct tttgaattgg catttggggt gattatcaac tgattaccat
    28681 tcacacgata atcaatgttt gcggcatttt ccacaacctc atcgaactct gacaaattca
    28741 gattattcgt atccgtcaca gtagtcgatt gccctaatac tgttttagcc gtactattat
    28801 ggaaggttgg tttcttctga taatcgtcaa tggcttgatt tatttttgcc tcaatggctg
    28861 cgatatttac ggcactacca tctgatcgtt ttattgagtg aagggtatac ccattaactt
    28921 cttgccagat catcttctgt gccacaatat gagtatctgc atctgtacct acttttttcc
    28981 ataaaacgga aactaatttg gctttatcgg acatatctgg taacgggttt ttctcatagc
    29041 caggagtgaa ctcattgtaa atcgggactc ctggttcaat acagaaaata tcttgtttaa
    29101 agccgttgta cactgcataa tgcggaacag tgacttctga cattggacga ccatcactaa
    29161 aagttccttc aacagcatat tgatgtgcta aatctagttc tacggttacc gtttgtggat
    29221 gggtaatttc atcagcaaaa gtaatcgtag gagcaattgc tccaacaatt gcttgaacag
    29281 ccaaactcaa aatagttaat acacataatt tctttttttg ttttttatac atgaaatgtt
    29341 ctcctttcaa attaagcaca aaaaaataag atgctcgctt gaacatcttt gtttataaag
    29401 tctttttaaa atttttattg actgaataga atggatttga gaatgaatga gcctaaaaat
    29461 ttaatctaaa atacgaaata tactgccttt aaattagaac acattttaga acacaaacat
    29521 ttcaaattta atcaaatata ataaaatata tttacgggaa aacctttata aatcaaggtt
    29581 tttcaaactt attcaatgat acgaagaaaa aaccaggtct taagaaagcc cgtaaagctt
    29641 cacagttctc aaaacgttaa ttgcgattat cgttcgagtt cacgagttac gagaatcgta
    29701 ctgggcgaag caatgctgag cgcagttacc ttaatcctaa cttttctggc actttccaca
    29761 atttggaaag tgcctttttt gtgtttttag ctatgttaat aggtaggatt cttggtctat
    29821 aatatcattc gatctataaa caaacgggaa aaagataaag tatcatcaaa caatattatt
    29881 tatccgcttc tgaggttgtg acagaagtgg tcagcttcaa gaaataagaa ggaatttaca
    29941 aaaattgctt ctcatgtttg ataaatattg tgacttgtga ccgaggagtt ggcaggagaa
    30001 caccgtttat tcggaagaag ggattgtgaa aagtcttttg tcataacctc ttatttggta
    30061 gctaattgta aatcccaaac ggcatgtatt atttttcata agtccgtttt taaggattaa
    30121 tgttttcttt tgctttttat atggtctggc ttcagcgaga agttccaacg catatacaag
    30181 gacgacttat gtcaacgata acaacgatta ccatggtggc cagtccactt ggattagcac
    30241 tgtttggtgc gctctttgat tggaagacaa cgcaaccacg tctgcgagac agtctaattt
    30301 ttctgggagg gagtcttgct atagttatcc ttataaccgg tctggtaaaa ttactcaagc
    30361 ttgatctgaa agaagcaaaa attttcagtg aagaggaata cagagaaacg actttggtcg
    30421 aaaagaaaga atcaggaaac tgatacaagc gtataacaaa ctgcctgata ataggaaaga
    30481 cacgtattgt ccgattgttt tttttgacat tcaattggaa aatttacact ttctttatac
    30541 atttctggct gctttcttta tacccgtcaa ctatacttca ctcatcaata agaaagaagg
    30601 ataagtatgg attttggaaa tgaaacggtc ttgaaagcaa ctggtataac caaaaaatac
    30661 ggtgctgcca aagcattaga taaagtatca atcgaaatca aacgtggaat gatctatgga
    30721 ttgattgggg aaaatggagc gggtaagtct acctttatga gaactattat gggtttgatc
    30781 tcgattgatg aaggttcgat cgaattattt ggaacgacgg atttgcaggc agcaagacga
    30841 cgtatgggtc aatcgattga aacccctgct ctttatccag aattaactgc aagagataac
    30901 ctacgtatcc aagcagcgaa cggcggtgtg tcggatagag aaatcgaaga cctgctgaaa
    30961 atgatgcgat tggaaaatac agggaaaaag aaagcaaaga atttctcttt aggtatgcgc
    31021 cagcgtttag ctattgcaaa tgcactgatc acgaatccag aatttttgat tttggatgaa
    31081 ccgacgaatg ggatggatcc agcagggatg gcagaaatgc gagaaatcat ccaacgtttg
    31141 gtaaaagaac ggggcatcac cgtcttgctt tctagccact tgctagatga attgtcacag
    31201 atcgccactc actatggtat tttgcatgaa ggacacttga tcaaagaatt atcgaaagaa
    31261 gaattggcac aagaatctcg tcaatttata aaaattgaca cttcggcgac ggaacaagca
    31321 gtaacggttc tcgactctct tggttatagg gattattttg tacaatctag ccgtgttatc
    31381 cagctttttg aaggaattga ccaagtagca gccatcaatc aagcattggt agaagcaaaa
    31441 gttcctgtgg atggtattca cctagttggg caaaaactgg aagattattt cttacaatta
    31501 actggaggca atccacatgt ttaatctatt aactgctgaa aaaataaaag tatggaagag
    31561 ccgtaaaatg tgggtctcac taggactgat ggtcgtactt ccgctttttt attcttggaa
    31621 cgaatggtat atgcatacaa aatacggcaa tgaattaaac caagcaacgg atacagtcat
    31681 caacggtgca acagggattt taatggtaga aaaaatggct gcatggattc ttctggcttt
    31741 cgcagcattt gcgtgctttt atattgggga agaatttcaa aacggtacga ttcgcaatac
    31801 gttatcttta ggacggaatc gaatgactta ttatgtgtcg aaacttctcg ttacgctttt
    31861 gatcacaaca cttggcgtgg ttttgatcac gggactagcc atgcttgctt acacacttgc
    31921 tttcggattt ggtgaagttg agggaatcaa aaactatgga aattatgttc taaaagtatt
    31981 tcctgtattg cttctgctga cattagggac attatctgtt ccagtggctt tgacattcat
    32041 cactagaagt accagtgtca gtttgcttct ttcttttctt tatatcatgg gaacggcctt
    32101 cattcccggt gttttcgcaa aaatcaaagg cttggaattt ctaacggagt ggtttacgga
    32161 aacgtggctc atgtatacgg actttgcgca acaagcaact tattcgcaag caccgaaaat
    32221 gattctggtc agtctagtaa caatcgtggt atcatcagct ctaggaatgt ttattttcca
    32281 aaaatctgac attaagtagg tggtcctgct ggaaactatt ttgattatcg aagacgatga
    32341 agctgttcat tccttactgg aggaggttct tgaacagaga tacaaacttc ttgatgctta
    32401 ttcaggtacg gaaggaaaat tattgttaag cacatatcct gtcgatttga tccttctaga
    32461 tctcatgctt ccagggcttt caggtgaagc attgttagca gaaatccgac agacatcgaa
    32521 tgttccgatc atcgttcttt cagcaaaaag cgatcaaaga gataaagttt ccttgttggc
    32581 agcaggagcg gatgactatg tgacgaaacc atttgatatc gaagaacttt tgttacgaat
    32641 cggcatccaa cttcgtcatc agacttctgt ccaagtaaaa gatctgtcgc aacgcatcta
    32701 ttacaaggaa atcttactgg acaaagagtc gagggatgta aatgtgaatg gtgtggctgt
    32761 ccatttgaca ggaagagaat tcgatctgct gaagcttttt ttagaacatc cgaaaaaagt
    32821 attttcgcga gcaaatcttt atgaaacagt ttggcaagaa ccatattttg atagtgagaa
    32881 aacagtgaat atgcatattt ccaatcttcg agggaagcta gcaaaggcac atgctgtgta
    32941 tattcatacg gtgtggggag tggggtttag atttgattaa atggctgttg cttctcgttg
    33001 ttatctttat gggaagtttt attttcttcc tgcaaaggga aatcaagaga ttaactaaga
    33061 aaatcgccac tctttctgaa cgacaagaat atggtggtcg tctttcagtc gaatttcgag
    33121 aaaagaatct aatggaccta gtagatgcac tgaatacatg gttggatgaa aatgaacaaa
    33181 aaggacaagc atttcaagaa atggaagaaa acatcagact atcgatcgtc ggcctttctc
    33241 atgatctcag aactcctttg actgctatta acggttatgt acaattactg gaacaggtag
    33301 aggatacgga gaaacgggag aagtatttaa cgatcatcaa acaatctgtt gcaagattac
    33361 tcagtatgac ggatcagttt tatgatcttg ctcgaataga gacgaagcaa aaagaaatgg
    33421 atctccatac gctttctttt tctaaagagg tggaagaagt atttttctct ttttatgaac
    33481 aattttctga gaaaaatatc gaggtagcgt tcgctgaaca ctcgataaat acaactgtat
    33541 tagcagatcc gctcatgttg ctgcgagtgc tgcaaaatat catacagaat ttattgcgtt
    33601 atgcagatgg acaagcaaag gtgacatttt caaaagagga tccattcatt cgattaacag
    33661 tggccaatcc tatcaaacca ggaacgcaaa cctctgttga aaaagtcttc cagcgttttt
    33721 ataccgaaaa taccagccgc acaaatacgg aatctagcgg aatcgggctt tacctgtcaa
    33781 aaaaattggt ggaaggaatg ggaggagaga tgaccgctag attagacggg ggcatcttct
    33841 ccatctccgt caagttgcgg agggtttaat tatacaagat tagtgagaac tagtagaaaa
    33901 atgtattatc ttatatatat ttaatagaga aaaaagcctg tgatataaat attgcaggct
    33961 tttttattga tttgaaataa tcaagaatat tagaaacttc tacattaatc aagaactttc
    34021 ttctgtatga aaatagagat aataattgta cacttaagct gtattcagga ggtgaagata
    34081 atgggcatcg aatttagcga acggcaaatg aaaatactat actttttgat tgaaaatgag
    34141 cacacaactt acaaagaatt atcagaacaa ttatatgtat ctacgaaaac aattagcaaa
    34201 gatattttaa ttttgaaaga aataatccaa aaaatgccag atgtacaaat gttggttcaa
    34261 cctaaaaaag gcgtttcgct tattggaaaa aaagaaacac ttcgaaattt attatccagt
    34321 cataaaatca atatcgcaga ctctccggag gaccgtctaa cttatgtgtt tgctaaatta
    34381 ttgaatacta atgattctct aaagattcaa aatcttgctg acgaattata cgtgagcagg
    34441 tcgacggtag aaagcacaat gaaagatgtt agaaaaagac taaacaagca tgggatgtcc
    34501 ttagtttcag atcgaaatgg cgtgcgcatc aaagctgatg aaaaaagtaa aagaaggttg
    34561 atgtcagaag ttgtgaacta ttattggggt ggctttacag catctaacag taaaacagat
    34621 ctcttagact tgaatatcaa tttcattggt gaagcgaata atttcttaaa taaggacata
    34681 ttgaaaaaag tagccgaatt gcttaatcga tacatgaata agacacaatt gaaatttaca
    34741 gattatgaat ttcaaacttt agctatccac cttgctattg cgatagaaag gattgaaaca
    34801 agcaatttct tatcacgtag taataattct gaagaagaaa tctcaaagaa tacacttatt
    34861 ttaatcaaaa tgattgaaaa agcattttca attattgtgc caatagatga acaagcttat
    34921 gttgacattc acattacttc gattgaaaaa aatgctatga atcacgactt agatcaaacg
    34981 aagctttcct atttagatat gggtgttgag ctacaagagt tagtaagaat taaattaaat
    35041 gaattttatc ctgatgaaga attgatcaat aatctagtat tacatctcaa tgcagcagtt
    35101 aaaagactca aattgggagt aaatatctat aacccttata cggacaaaat caaatattct
    35161 tttaaaaggt catttatgat aagtgtagat ttattagagg aaatcgaaga gagattctgt
    35221 atacatttta atgaagatga aatcgcttac gtaacactcc atattcaatc acttttggat
    35281 cgttacaaac ctgacaaaac gaaagtgata ttagtttgta gtagcggata tggaacatca
    35341 aaattattag agcaaagaat aaataacggt tttgcagata tggttgagat agaagatgta
    35401 ctaagcatca atgaactaca agatagaaaa gtcactgatg aacttgttat aagcacgatt
    35461 ccgattgaaa atacaaattt tcctgttatt gttgttagtc ctttgatgtt aggggcagat
    35521 gtcagaaaag taaaacagta tctacagtta aatgaacaaa cagaagatgc ttttctaaat
    35581 ttattagata aacgattttg cttttttacc caccaaataa aggataatca agtgaacgtt
    35641 ttaaatataa ttaccaaaaa attggtaaaa gaaaattacg cgaaagaagg gatactagag
    35701 ggagcaatca aaagagaaaa actatcttca acagcgatga atgattttgc tatgccgcat
    35761 atcgaagtag attttataaa aaagccagta atctgtgtct acgttaatcc agaaggaatt
    35821 gattgggatg ggacacttgt tcattctgtt ttcttttttg cacttaatga aaaagtaaaa
    35881 cctgaattga ataaaattta tgaaacattc aatgaaatcg cttcgaatca tgagttatta
    35941 gcaaatttaa aaacgagttc gtcgtttgaa aaattagttt atatattgaa agaaggctta
    36001 taaatgatca gtgaaaaaat caatataaat aatatagtga tagatttaaa tgcatctgat
    36061 aaaaaaagtg tagtcgaaaa attatcgaaa ttgttagaga ctaatgggtt tgtgtctgac
    36121 cacaaacaat ttcaaaaaga cgtatttgac agagaaaacc atatgaccac aggaattggg
    36181 aaaaatatcg ccattcctca tggcaaaagc ctagcagtaa ttgaatcaac agtggctgta
    36241 gccaagttaa agcggtctgt tgaatggggc gcattagatg atcaaccagt tgatctcgtc
    36301 tttttgttag caataaaaga cactgataaa ggtactaatc atttacgtat tttagcagaa
    36361 ttatctggaa gattgatgga tgacgagttt gttaaaagaa taaaagattc gagaaataaa
    36421 aaagaattgg taaaagctct atcattttaa aaggaggaat ttcatatgac aaaaatagta
    36481 gcagtaacag catgtatcgc agggattgcc catacttata tggcaaaagc taatttagaa
    36541 aagtttgcga agaagaaagg gttagatatt aaagtagaaa cacagggggc tatggggata
    36601 gaaaacaaac tttcaatttc ggatgtttcc gaggcagatg ttgtcatatt tgcagtagat
    36661 acaaatgttt cagaacgagg tcgctttgac ggtaaaccga ttttaacagt agggacctca
    36721 gaggtagtaa aagatggtga agagacgatt gatgaagcaa ttgcattaat taacaaataa
    36781 aaaatatatc gtgggggagt tactaatgga attcaagaaa caaggaagag aaattctaaa
    36841 tcatttccaa agtggggttt cctatatgat cccgcttgtc gtagcagcag ggttactaac
    36901 ttctattgca gtcatttttg gtggcacagg tgtttgggac cagacggata cattctgggg
    36961 agtcctgcga atgattggtc agacgggatt acagtttatc gtaccgatga tttcagccta
    37021 tatcgcatat tcaattgctg accgtcccgg tctagcacca gcttttatca ctgggatgat
    37081 gtgtcaaaat cttggtatgg gatttattgg aggaatggtt gctggattag cttgtggcta
    37141 tttagctaat gcattgaaaa aagtaaaagt accgatgcaa gttattagtc taaaatcaat
    37201 tatgctgatt ccttttgtta ctacattagt tgttggaatc atattatggt atgtactagc
    37261 tacgcctatt caagcaatga caacaggact gactaattgg ctagaaggca tgtcaggagc
    37321 aaatgctgct ttgatgtcgg ctattttagg aggaatgatg gcgtttgata tgggaggacc
    37381 tatcaataaa atcgcatatg cttttggggt tgcttctttt tcaagcggaa gctttggacc
    37441 tagtacagcc atgttattag gaattgctat tccgccattt ggaatgtttt tagcaacaat
    37501 tttaaacaaa aaactttatg atgaatcgga acgagataat gcaaagacag ctatgattat
    37561 gggccttgtg gggattactg aagggacgat cccgtttgcc gtgaaagatc ctttacgagt
    37621 aataccaagc atcgtggtgg gcactgcagt agcatgtggt ctaaatggat tttttggtgt
    37681 tactcaagaa acaatgcttt caacctttat ggcaattcct tttgttacga atataccttt
    37741 atatttagta tcaattgtta ttgggggagt ggttacagcg ttaatggtca atgcactaaa
    37801 atccatcaag tataaaaaag aacaacaagt aaattcagta gaagaaggaa agtaggagaa
    37861 tctgatatgg taaatgcaca tataattcaa cacacgcact gggacagaga atggtatttt
    37921 acaaatgaag atgccatcgt attgtctgat caggtcttta ctgaagttct agatgaattg
    37981 gaaaggaacc ctaaagtaaa cttttgttta gatggacaaa cttcaatcgt cgatgagtat
    38041 ttggagatca atccgcaaaa gcttaaggtt atccaacgac tgatgaaaac aggacaacta
    38101 ttcgtgggac cgtggtatgc acaaacagat gccttattag tggatgctga atcaatttta
    38161 agaaacttaa tgattggaat cagtgatacg atccttaaat atggagatcc aatgatggta
    38221 gggtatttgc ctgacacgtt tgggttcaat gcgaatttgc ctacactttt gcatcaagta
    38281 gaaatcgata atttcatgtg ttggcgagga ttaaactttg aaacgatggt tccatctcct
    38341 tattttatct ggaaaggatt gggagataaa tttgtttatg ccatgaattt tccatttggt
    38401 tatatgactg gaatgatgac cttagacgca attaaagata ttcctgaatt tgcaaaagaa
    38461 aagttggatg aagcagttga aatgcttcat atgcaaggaa aaaacaaaga tattttagta
    38521 ccttcgggtg tagatcaaaa gtcaatgatt ttaaattttg atcaagtcgt agagaagttg
    38581 aataaataca gtaaattcca caatattatt tcagattatc cttcttatgt agaagttatc
    38641 agaaacaaaa aagaattacc tgaatataaa ggtgaacttc gtcagccggt ttactctcgt
    38701 gttcatcgtt ctattggctc agtaagaaca aaaatgaaaa tagaaaattt taaattggaa
    38761 caaaaaattc taaaacgagt tgagccgctc atgctgattg cagaaagaaa tggtatccat
    38821 atcagtaaag gcctacttcg tcggttatgg aaaaagattt tgcaaaacca ggctcatgat
    38881 agtattggtg gctgtgtttc agataatgtt gcggaagata ttttccatcg aatcaaagaa
    38941 gctaatgaga tagctgacgg aatcgaaaac cttattgtaa aacggatggc tgatgcatta
    39001 aagctaaaat cgaatgaagt ccttgttttc aacacaacga ctgagaatta tagtggatcg
    39061 aaaatcgtgc atgtagtagc tgattcaaag aaaatacact tcaaaggtag tccctataat
    39121 atcattgaaa aagaagttta ttttccagaa cggaaaaatg cttatcggat gattgctaca
    39181 ggtttcgaat attttgatga accagcttat tatgaacttg acataaaaat aaatgtaaat
    39241 gtgccagcac taggatacac tgttgttgaa tttgagaaaa ataatgaaaa attagaaaca
    39301 gcaatcgaga tagacaaaac tgagataagt aacaataaat ataaactttc ttttaaagat
    39361 gggcaattga atttgatagt tggagataga caatatcttg atttcgttca tttgatcgat
    39421 tcagcaaatg atggcgatac gtatgattat tcaccattag aaggagatac agaactatct
    39481 ctaaaattag aaacagctaa agtatataaa gattctttac aagaaacatt agttgtttac
    39541 ggaaaagctc aacttcctaa agatttaaaa gatcgtttat ctgaaaaacc cgaaatggaa
    39601 gaaatatctt atgaaatatc gttcagtctg ggagaaagtc aaatcgttga aggcacactc
    39661 aaaattcata atactgttta ctcgcatcgt atgcgcttag caattaatgt tttagataaa
    39721 tctgaaaaat caattgcaca aattcaaaac ggatttattg aaaatacccc tgatgtcatt
    39781 cctccgaatt ggtcagaaac tatggtagag aaacctgtta atttagaaat attcgataag
    39841 agtgtctctg tggcagaaaa tgatcattat tttacgtttt atgcagatgg tttaaaagag
    39901 tatgaacgta tagaaaatca attaatcata accttatttt caacaacagg ggaacttgga
    39961 aaacctaatc ttgcttggcg acctgggcga gcttcaggag atacgacaaa tgaagggcat
    40021 gtcatgatgg aaacaccgct agctcaagag ataggtgagt ataaagtcac ttttggattt
    40081 aatgtagaag aaggaagatt taatgagttc aaagtggcaa aacaggcaga aaaacggtta
    40141 gaacaatcta tttcttatca aaagcagaaa ctgaatgtat ttattcacag gttagataac
    40201 aagatttggc cattacaaaa tgaacttgat gttcctcgta aattttcatt gattgaatta
    40261 cctgaagact taattgtatc atcgatttat ccttcttatt ttcatgaaca aggatttgtt
    40321 cttcgcttag ctaatccaac agaacaagaa aaaatagtac ctgaggctat cctttccctt
    40381 gggacagttg tcaatgcact ggagaataaa caagaattaa caacaattcc tccctatgat
    40441 tatttgtcaa ttttgataaa tgagagataa atatgtaata tacaagaaaa aaacaccttt
    40501 tattttgaaa ggtgtttttt taccctcttt tcaaatagcg caacagtgct atactaaagc
    40561 taacaaataa aggaacggag agggatatgg gtcaaagaga atttatcgtt ttaattatta
    40621 ttgcagctgt tattttatta gttgctttag acattttttc acgtttgaaa ttaaaggaaa
    40681 cagttcgtag caaatgggaa aaaattcctt atcaaccgag atttgataaa gaagaaagct
    40741 tgaaggaagc atggctcacg gagaagaagt tccgttcatg ggatagcgaa atcgatgatc
    40801 ttacttggta tgacctggat atgttcgaag tattcgaagg aatcaacagt acctattcta
    40861 gtgtaggttc agaagcgttg tatcaacgtt tacgcagctt tgattttggt gaagatcagc
    40921 agttagaaaa actgattgct ttttatcagg aaaatcctca actgagagaa aaaatccaat
    40981 atcaattcgc tcgtttagga aaaaaagatc ataattttgc aaaacaatat ctagcggatg
    41041 gaaaaagtaa aaagattgga cgcgtaggtt tatttatttt tcttggcttg cttccgctgg
    41101 tgggtttggg tttgcttctt ttaggacaag tactaggaat ctatttggca atcggtgggt
    41161 tagtattcaa tacggtctat tatctagtga agaaacaaac actagaaaca gagctgaata
    41221 gcatgagtta tttggtttca acgatttcag ccgccaatca gctagcaaaa ctgccgacac
    41281 cacttcaaga agagctgaaa cagcaaacta aaccattaag agacatccct aaattaggtt
    41341 tttcatttcg tatgaagagt gggagtgaag cagagttgat ttttgaatat gtcaacatca
    41401 tgttcatgct gccatttatc tcctatcatt tcgttctcac taaattagaa aagcattcaa
    41461 ctgaagccat caagctgtgg gaattgctgg gagaattaga agtagccgct gcaatattga
    41521 attaccgtac ttatatgccc atcacttgcc gaccagagtt tactgagggc ggtgtgacag
    41581 cgaaagatat ctatcatcca ttgttaaaag aagcggtagt gaatccggtc aactggcaga
    41641 agaatacatt agtcacgggt tccaatgcat caggaaaatc tacgtatgtc aaaagtatag
    41701 ccatcagctg tattctagca cagacgatcc aaacagcgat tgcagaacag tttaccctgc
    41761 aacctggaca tgtgttgact tccatggcaa tcgaagatga tttatttgaa ggcgacagtt
    41821 attttgtttc agaaatcaaa tcgatcaaac gtttgttgga ccaagtggct tccaaagaac
    41881 gctgctactg cttcgtagat gagattttaa aagggacaaa tacgatcgaa cggattgctt
    41941 cttccgcttc agtcgttcgg tggcttgcgg agtatccatc tttagcattt gtcgccacac
    42001 atgatatcga attgacagag attttaaaga attattgtga gaatgtccac tttgaagaac
    42061 aagtaacaga aggaaaagga atcagttttg atttcaaatt aaaagaagga ccttcaagga
    42121 cccgaaatgc gattgccttg ctgaaagttt tggattaccc agagacttta gtagaaaccg
    42181 caaaaacgga agccgcgtta tttgatgaaa agcggcagtg gcatgtattg ggataggcga
    42241 agtgatcgaa agtatttatt ggatcaaaaa tcttggcttt tgcttccgcc gtttatacgt
    42301 gtttagagcg tgagacaaaa gtgttcttta cttttgtctc acgctccttt tgttgcacat
    42361 atgaatagta atcctttatc ttcgatttgt tttttctgaa aaggttgtct gacttcgtcc
    42421 gattgatgtc tttcattagg agattttcct gagcccagca acctgatttt tcgctggaac
    42481 caagttgttt tttcatctgc tgtattccat gatacaataa agaatcatgt ctataaggag
    42541 ataggaaata aatggcaaat gcgcttgaaa taaataattt gaaaaaggtt tatggaagtg
    42601 gggtcgaagc attgcgagga atcgatcttt ccgtgaaaga aggagatttc tatgcgctat
    42661 taggaccaaa tggagctggg aagtctacaa cgatcggaat cattacttct ttagtcaata
    42721 aaacatctgg aaaagtgaat gtcttcggtt atgatctgga tacggatcta gtccgagcaa
    42781 agcaacagat tggattagtc ccgcaagagt tcaatttcaa tccctttgaa acggtccagc
    42841 aaatcgtcgt gaatcaagca ggctactacg gtgtctctcg caaagaagcg atgaaacgca
    42901 gcgaaaaata tttgaagcag tccaatcttt gggaaaagag aaatgtccgt gcacgtatgc
    42961 tttccggggg aatgaagcga agactgatga ttgctagggc attgatgcat gaaccacgtt
    43021 tgctgatctt ggatgaacca acagcaggag tcgatattga attgagacgc gagatgtggg
    43081 agtttctccg agaattgaac gaaaatggta caacgatcat tttgacgacg cattatttag
    43141 aagaagctga aatgctttgt cgcaacatcg ggatcatcca atcaggtgaa ctgatcgaga
    43201 atactagtat gaaagagctt ctatccaaac tgcaatttga aacatttatc tttgatttag
    43261 cgccatatga gaccaaacct gtgattgaag gatacgcttg tacatttgaa gatgaacaga
    43321 caatcgctgt cgaagttgaa cgaaatcaag gggtgaacgg aatcttcgat cagctgacaa
    43381 gacaaggaat caaagttctt tctatgagga ataagtcgaa tcgtttagaa gaactgtttt
    43441 tgaaaatcac agaagaaaat aatcatacgg aggagaaaaa tgtttaatct ttattttact
    43501 gcattaaaaa gtttagctat caaggaaacg aaccgctatt tgcgtatctg ggtccaaaca
    43561 ctggttccgc ctgtgatcac gacatccttg tactttgtta ttttcggaaa aatgatcggt
    43621 ggtcggatcg gtgacatggg tggtttttca tatatggaat tcatcgtgcc tggtttgatt
    43681 atgatgtctg ctatcactag ttcgtatgcc aacgtttctt cgtccttctt ctctcaaaaa
    43741 ttccagaaaa acatcgaaga aatcttggta gcgcctgttc ctacgcatgt gatcatttgg
    43801 ggctttgtga ttggcggagt gggtcgcagt attctcgtcg gctctctcgt aacgatcatt
    43861 tccttgttct ttgtaccact tcatgtgtat tcatggttca tggtcatcat tacattattg
    43921 atgacggcaa tcttgttttc gttagcagga ctgctcaacg ggatttttgc tcagtcttat
    43981 gacgatgtat cgatcgtgcc tacatttgtc ttgcagccgt taacttatct aggtggcgtt
    44041 ttctatgcca tctctatgtt gccgccattt tggcaagctg tttcgaaagt caatccgatc
    44101 gtttatatga tctccggttt ccgttacgga ttccttggaa caatcgatgt gccagtagca
    44161 acatcgatga tcgtacttgt gctattcatc gttgttttgt acgccgttac ttggtatttg
    44221 atcaataaag gaagaggatt gagaagttag aaaagaggtt gtgaaatcag cgttttgacc
    44281 tgagtattag agaaacaaac gggaaaatag ttcctcatat tttgtcgttt gttgagctaa
    44341 taccgagggt cagctgattg aacaccgttt agtggagagg ttgtgaaaaa gctgttctgc
    44401 atcaagtaat aagaacagcc aaacaaaaat agctcctcct atttttcgtt tggttggact
    44461 tattaccgca gatgcaagct tttgaacacc atttattccg gaaaaggaat tgtgataaga
    44521 tttttgtcac agtctttttt tgctcaatca gaaacatctt ttaagctgtt cactttgctt
    44581 ctttttttgt ttcctagtat aatgacaatg tttttgagaa agattgcgac ttcactgctt
    44641 gatagaatgg agagtaacaa ataaatgact actaattttt ggaaagagct gccaaagcct
    44701 ttttttgttt tagcaccaat ggaagatgta acagatgttg tatttcgcca tgtcgtaaag
    44761 catgcagcag caccggatgt cttctttact gagtttacaa actcagacag tttctgtcat
    44821 ccagatggaa aagacagtgt acgaggaaga ttgaccttta cagaagatga acagccgatg
    44881 gttgcccata tttggggaga caagccagaa ttttttagag aaatgagcat tgctatggca
    44941 gaaatgggct tccaaggaat cgatatcaac atgggctgcc cagtccctaa tgtagcagaa
    45001 cgtggaaaag gcagtggctt gatccttcgc ccagaagtag cggcacaatt gattgaagcg
    45061 gctaaagctg gcggtttgcc tgtcagcgtc aagacgcgta tcggctatac ggaaatgtct
    45121 gagatggaag aatggatcag tcacctactt catcaagata tagccaatct ttctgtccat
    45181 ttgcgtacgc gtaaagagat gagtaaagta gatgcacatt gggaagtcat ccctaaaatc
    45241 attgctttac gtgaccaaat cgctccgcaa acactgatca cgatcaacgg agatatccca
    45301 gatcgccaga caggagaaga attggccgaa aaatatggcg tagatggcat catgattggc
    45361 cgggggattt tcaagaatcc ttatgcattt gaaaaagaac cgagggaaca tacagaaaaa
    45421 gaactgatcg gactacttcg tctgcaacta gatctgcaag atcattattc agaaatcatc
    45481 ccgcgttcca tcacaggact acatcgtttc tttaaaatct atatcaaagg atttccagga
    45541 gcaaatgact tgcgagttca gttgatgaat acgaaatcta ctgatgaagt acgagagatc
    45601 ttagacacct ttgaaaaaga acatccaacg cttttcagtg aacaatgatt tcttgcttga
    45661 acaccgtgaa ttagaggtcg tgaaaaagct gtcctgcatc aagtaataag aacagccaaa
    45721 caaaaatagc tcctcatatt ttgtcgtttt ttgagcgaat accgagggtc agttgattga
    45781 acaccgttta aaagaggttg tgacaaaagt cttgtcgcaa cctctttttt aaccaaaaaa
    45841 gtgcaaatag tttactttaa gtaagagaat aattatgatg aataaaagtg gaacaaatag
    45901 catactatat atattgaaat gaaaactgta tggatgctcc aacttgatga agctaaggag
    45961 gaattcgtat gtcagaaaag gaattaatga aggtagtcgg tttttatgaa gggttgtcga
    46021 tcgatgatcc aaaaagcttt attgatgaaa aagaacagat acctgtaccc agttcacggg
    46081 atcttctggt aaaagtcaac gcagtatctg ttaatcctgt cgatacgaaa cttcgccaag
    46141 ataacgggat aagaaatgct ttacgtatct tagggtttga tggagtcggg aaagttgttg
    46201 ctgtaggtga tgaagtgcaa aaattttctg tcggcgatcg cgttttttac gcaggtacaa
    46261 cgacaagagc aggaagcaat caagaatacc aactggttga cgaacgtatt gtcgctcttg
    46321 caccaaaaaa tctttcagat gaagaagccg cagccttgcc gttgacctca ttgaccgctt
    46381 atgaattgtt atttgaaaaa tttggtctga ctccagaaga aaatgccaac cgcgggaaaa
    46441 aaatcttagt gatcaacgga gctgggggcg tcggttcgat tttgaatcaa ctagcgcatt
    46501 gggctggcct agaagtctac gcgactgcaa gtccgaagaa ttttgaatgg ctgaaaaaaa
    46561 caggtgtaga ctatccaatc gattatcatc acgacttaag taaaagtttt cgtgaattag
    46621 gaatcgatca agtagagtat gtcgctgtat tattcgatat tacccgctac tttgatcaaa
    46681 tcaaacacct tgtccgacca tttggacatg tgggaacgat cgtagggatc aaagagccgt
    46741 tagatatagg tgtttggaaa aaccaatcta tcagcttcga ctgggagtat atgttcgcta
    46801 aaacagacta taactatgaa atcgaaacgc aaggagaagc tttagcgcac attgctgctt
    46861 tagcaaatga agggattatc cgcagtacat tgagcaaggt ctattcagat gggatgaatg
    46921 cgaagaactt gaaacaagca accaaagacg tagaaacagg acatatatta ggaaaagtcg
    46981 ttgttagtgg tccgttcaac ggataagcaa gaatacgccg atttttttct tatttgactt
    47041 tgataaaaac aagagcgcat gacagtaatt tgccataagc tcttgttttt ttatttataa
    47101 taagcgaatc aacgaaaggg aaagaattcc taccaagacg atcaccaata agctttttga
    47161 aagaatagca gctaaaagtg taggaactga agccaaagca ttttcgagat tgatctgcgg
    47221 cagatgtccg atattttggg taaatagact gctgaaccat aaagcagaca taatgacgat
    47281 cgggacaaaa cttaaatact caagcaacgg ttgcggcaaa tcgaattttt tcagtaagac
    47341 aaatggtagg atcctagaaa gccaagttgc taaagtacaa cctaaaatag tcagcaatgt
    47401 aaactcaaaa gaaggcatgt ttgatccaca cccccaatcc gcacccaatc agtgttacaa
    47461 ctaaaatcac taagttactt gggataaaaa tcagtccgac atataccaga atcaaagtga
    47521 gaccaatcag gatcaactgt aatcgtttag aggtgtttct atcagaaatg acttgaagat
    47581 aaagtaaccc gataaacata gcgacgatgg cgaaatccaa accaaacttt ttcggatcag
    47641 taataaaatt ccctaaaagt gcgccaacaa gagaagaacc cacccacgtc aaataagaaa
    47701 tccaattagc tgtattcatc caacgaaagt ttaacttttg tccagtatag ttcaatttat
    47761 tcattcccag tgcgaaactt tcatctgtca gcaatgtgcc aatcagcaga ttttgaagca
    47821 gccgattttt cttgaaataa ggagcaatcg tcatactcat cagaatcatt cgggaattga
    47881 ctagaaaggt cgagagaacg atggagagca gtggactatg gctagcaagc atgctgaccg
    47941 tgataaattg ggcagaacca gcatagatca gcagggacat caacgtgacg accagcggat
    48001 gaaacccggc agctttccca acgataccaa atgcaagtcc aataccgata taaccaaaaa
    48061 cggtaggtaa cgtgtctttg atcgcagttt ttatatctaa cttttcatcc aataacaaaa
    48121 ctcctttagc acgacataaa ttttcataat tgtcgtcaaa cttgtgaata ttctaaattt
    48181 ttaatgaaga cttgtcaact tcaattttaa acaaataaaa aagactggac gtagtttaaa
    48241 aaaatcttct caagttagat ttccagacct aacttctgag gatcactaca tttgtcacag
    48301 tctcttatac tgacaagtat aaatgaagta tgaagaaaaa gtatttgtta ttggtatttc
    48361 tcattattct tgttttctaa tgtgttcact tcaagtggaa ttttagaata ttattcaact
    48421 aacaagtcta catcgtatct aaatccattt gtatcactta attgcttgta aaattcgcca
    48481 ctttttttaa ttgttctttt ttgagtagta aggtcgagtg aaaccagccc atatcggttt
    48541 ttataggcgt tgatccatga ccagcaatca ataaatgtcc aaagatgata ccccttaatg
    48601 ttacatcctt catttattgc cttccataag taagcaagat gatctttgat atactcaatc
    48661 cgataatcat caattatttg ccctgtatca tccatgaaac gttcttcatc cgcaacgccc
    48721 attccgttct ccgagataaa tgcttcgatg tttccataat ggttcttgat atccatgcac
    48781 aaatcataaa ttcctttttc gtaaatttcc catccccggt attcgttcat tcgtcgtcca
    48841 ggcatgatat gtggttcgaa aaaccactct ggtaaaaatg gactagatgt atccaccata
    48901 gaagaacgag atttgacgcg acgtggctcg taatagttta gtccaagaat ttgagcggta
    48961 ttttcgtaga tacatacaag atcttctttc gtataatctt caggaagctg attatattct
    49021 tgtaataaca ggataagctt tttagaaaaa ttccctttaa ccataggatc taaaaagctc
    49081 cgcgtataaa ataaatcagc gatttctgct gctttcacat cagcaggatt ttgactacga
    49141 ggaaaagtag gactcaaatt tagaataacg cctattttac taggaagagc tagttcttta
    49201 aatacttgta ttgcttttgc gtgtgcaagt atagtgaaga atgctgcttg cgctccgcgt
    49261 ttaaagtcaa tgaggtttgg ataatgaaaa tcttcaagat aagtacctaa aataggccca
    49321 agtggttcat tgaaggtaaa ccaatgacaa actaagtcac caaattcttt aaaacaagtt
    49381 tcagcgtaat gaacataagc atcaactact tcacgtgatt cccatcctcc tttatcttga
    49441 agagtagcag gcatgtcaaa atgatataag ttggcaaata ctttgattcc gttattattc
    49501 atttcggaaa ataattttcg atagaaagaa atggctttgg gatttacttt tccggtaccg
    49561 ccatttggaa acattcgtgc ccaagaaata gaaacgcgaa aggagttatg cccagttttt
    49621 ttcattagtt ctatatcttc tttatattga tggatgtgat cggttgtaat agaggaatcg
    49681 attcgttgat aaaaccggta aggttgttct ttaaaccagc gatcccagat agtctctgct
    49741 tttcctgttt ctccttttcc ttctgcttgc tcagctgacc aagcggaacc ccaccaaaaa
    49801 tcatctggaa aagttagctt tttcatagtt gtcataattt ttttacctcc tgtttccact
    49861 taatgataac accgcttaca acttaaagta aatagttttg tttaaatata tatatctttt
    49921 ttattgtgtg tataattttg atgtggattt tatcggttta tttatgttaa agtatagatg
    49981 aagatatgtt tttgattgga gggtcaccat tgaaaaaaat tgcgaagtat atggaaattt
    50041 acttagatat aaagaaaaaa attgtaaatg gagagtataa aattggtgag aaacttccta
    50101 caggagatga attggcagtt atttatcata caagcaagtt aacagtaaag aaaggcgtag
    50161 acttacttgt ttccgaagga gttttgcgaa gtagaagtgg gtatgggact gaagtcgtca
    50221 gaacaccaat tgataactca cgagttcttg gtccaaacga aggtttgttt agcgtggttg
    50281 gtgaagaaca cgtagattca gaaatccata ctttctcaat tgaacttcca tcgaaaaaag
    50341 tagctgataa attaagtatc agtgtaaaag actatgtata taatattgta cgaagccgtt
    50401 ttattgattg tcaaccatac tcgattgagc aaacattcat gccattagca gtcataccag
    50461 gcttggaacc aaaacattta aagaaatctg tatatgctta tatcacaaat gaactgggtc
    50521 tggaaataaa gacttcgcac atacgtatca aaggagatac tgcgaatgaa tttgacgcaa
    50581 aaatattaaa aatagatcca ggtgaattta tgattgagat agacaaagtt gttgctctat
    50641 cgtctggtaa acctttcgag tattcggtat cgagacattt atatcaagac tttgtttttg
    50701 aagctgtttt tgttgaaaaa taagctcggg aaaattattt tttcagagct tattttttta
    50761 tactaaaaaa gatatatata tttaattaaa agtatttact ttttttgata agtgatgtat
    50821 tatttgggtg gaaaaagtaa tcgattacaa taagcataaa ggagaaaaaa tatgggcaaa
    50881 agtagcagga aggacattgt atttgaaaaa tttggtatag ttgcgactaa gttggggaat
    50941 cagatccatt taagaacttt gcgagatgcg tttgctacat ttatgccatt tatgatgtta
    51001 gcaggatttg tgacacttat taattcagtg atcttagaac caacaggatt catgggggaa
    51061 attgtcgata ctagtacatt gaccacgtta caacaaattg gtacttcaat tgccaatggt
    51121 acattaagca taactacatt attagttgta gcagcagtat cttatcatat gtgtgcaagc
    51181 agaaattata caaatcatat tgcagcggta ttagttgcta tttcgacatt tgttgtatta
    51241 acacctatgc agatgatgtt tactcctgaa aatgcggata aagctgttga agtgacgggt
    51301 gtgataccta tatcctatac aggagcttca ggaatgtttg taggtatatt tgttggatta
    51361 gcggctacag aattatttat taaactttct aataataaga aactacagat tcagctatcc
    51421 ggaaatattc ctcccgctgt tttaaagtct tttaatgtat taatcccaat cattattacg
    51481 attacctcgt ttgctttatt gtcatttata atcattcaat tgtttaatat ggatataaat
    51541 aagctaatta ccacgatagt tacagagcca ttaagtaaaa ttacaactgg tttaccagga
    51601 tttttgttaa ttacatctat tgctaatcta ttttttggat ttggtatcca tcaagcagta
    51661 atatcaggtt cattgttaga tccgtttttg attcagaata tgcaagaaaa tatggcagct
    51721 tatgcaaaca aagagcaaat tccacatatt atcaatatgg cctttaaaga tacctttgcg
    51781 gtaatgggtg gatcgggaaa tacgattgct ttattgattg caatatttat cttcagtcgt
    51841 agaaaagatt ataaggactt tgctaaatta tcggtaactc ccgctatatt caatataagc
    51901 gaacctatta tttttggatt gcctattgta tttaatatca gtctgattat tccctttgtg
    51961 cttgcaccga ttttttcttt gacagtagca tattttgcaa ctgctgtggg attaattaat
    52021 catgtagtag tgcaaattcc ttggactacg ccaccagtta tatcgggatt tttagcaaca
    52081 gccggagact ggcgagcagc tgttttacaa atattaatta tagccgcaag tgtgtttata
    52141 tatttaccgt ttttacgaat tgacgaacac gtgacaacta aattaagtga aaatgaaaca
    52201 taatttacta tgagataaca aaaattaaca caagaagtcg ttggaatata aatagccagt
    52261 aatttcaata aggatcatat tttttgataa gtacgaacat
//
LOCUS       DAZBTF010000013        44988 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000013 DAZBTF010000000
VERSION     DAZBTF010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.013"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            32..2629
                     /gene="adhE"
                     /locus_tag="ACHGNS_000821"
                     /gene_synonym="adhC"
     CDS             32..2629
                     /gene="adhE"
                     /locus_tag="ACHGNS_000821"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFN6936785.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2858..3964
                     /locus_tag="ACHGNS_000822"
     CDS             2858..3964
                     /locus_tag="ACHGNS_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFN6936786.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            3981..5009
                     /locus_tag="ACHGNS_000823"
     CDS             3981..5009
                     /locus_tag="ACHGNS_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFN6936787.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            5015..5494
                     /locus_tag="ACHGNS_000824"
     CDS             5015..5494
                     /locus_tag="ACHGNS_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFN6936788.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5510..6805
                     /locus_tag="ACHGNS_000825"
     CDS             5510..6805
                     /locus_tag="ACHGNS_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFN6936789.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            6816..7640
                     /locus_tag="ACHGNS_000826"
     CDS             6816..7640
                     /locus_tag="ACHGNS_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFN6936790.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            7805..8704
                     /locus_tag="ACHGNS_000827"
     CDS             7805..8704
                     /locus_tag="ACHGNS_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6936791.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(8830..9699)
                     /locus_tag="ACHGNS_000828"
     CDS             complement(8830..9699)
                     /locus_tag="ACHGNS_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFN6936792.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            9852..11312
                     /gene="rhaB"
                     /locus_tag="ACHGNS_000829"
     CDS             9852..11312
                     /gene="rhaB"
                     /locus_tag="ACHGNS_000829"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFN6936793.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            11309..12565
                     /gene="rhaA"
                     /locus_tag="ACHGNS_000830"
     CDS             11309..12565
                     /gene="rhaA"
                     /locus_tag="ACHGNS_000830"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFN6936794.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            12579..13424
                     /gene="rhaD"
                     /locus_tag="ACHGNS_000831"
     CDS             12579..13424
                     /gene="rhaD"
                     /locus_tag="ACHGNS_000831"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFN6936795.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            13426..13737
                     /gene="rhaM"
                     /locus_tag="ACHGNS_000832"
     CDS             13426..13737
                     /gene="rhaM"
                     /locus_tag="ACHGNS_000832"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFN6936796.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            13819..14970
                     /gene="fucO"
                     /locus_tag="ACHGNS_000833"
     CDS             13819..14970
                     /gene="fucO"
                     /locus_tag="ACHGNS_000833"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFN6936797.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            15097..15855
                     /locus_tag="ACHGNS_000834"
     CDS             15097..15855
                     /locus_tag="ACHGNS_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFN6936798.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            15955..16599
                     /locus_tag="ACHGNS_000835"
     CDS             15955..16599
                     /locus_tag="ACHGNS_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFN6936799.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            16606..17613
                     /locus_tag="ACHGNS_000836"
     CDS             16606..17613
                     /locus_tag="ACHGNS_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFN6936800.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            17629..18471
                     /gene="kduI"
                     /locus_tag="ACHGNS_000837"
     CDS             17629..18471
                     /gene="kduI"
                     /locus_tag="ACHGNS_000837"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFN6936801.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            18491..19294
                     /locus_tag="ACHGNS_000838"
     CDS             18491..19294
                     /locus_tag="ACHGNS_000838"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFN6936802.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(19543..20607)
                     /gene="fni"
                     /locus_tag="ACHGNS_000839"
     CDS             complement(19543..20607)
                     /gene="fni"
                     /locus_tag="ACHGNS_000839"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFN6936803.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(20604..21689)
                     /locus_tag="ACHGNS_000840"
     CDS             complement(20604..21689)
                     /locus_tag="ACHGNS_000840"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFN6936804.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(21702..22679)
                     /gene="mvaD"
                     /locus_tag="ACHGNS_000841"
     CDS             complement(21702..22679)
                     /gene="mvaD"
                     /locus_tag="ACHGNS_000841"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFN6936805.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(22672..23616)
                     /gene="mvk"
                     /locus_tag="ACHGNS_000842"
     CDS             complement(22672..23616)
                     /gene="mvk"
                     /locus_tag="ACHGNS_000842"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFN6936806.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            23932..24591
                     /locus_tag="ACHGNS_000843"
     CDS             23932..24591
                     /locus_tag="ACHGNS_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFN6936807.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            24676..25581
                     /locus_tag="ACHGNS_000844"
     CDS             24676..25581
                     /locus_tag="ACHGNS_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFN6936808.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(25583..26215)
                     /locus_tag="ACHGNS_000845"
     CDS             complement(25583..26215)
                     /locus_tag="ACHGNS_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFN6936809.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    26366..26491
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            26535..27059
                     /gene="infC"
                     /locus_tag="ACHGNS_000846"
     CDS             26535..27059
                     /gene="infC"
                     /locus_tag="ACHGNS_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFN6936810.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            27131..27331
                     /gene="rpmI"
                     /locus_tag="ACHGNS_000847"
     CDS             27131..27331
                     /gene="rpmI"
                     /locus_tag="ACHGNS_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFN6936811.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            27384..27743
                     /gene="rplT"
                     /locus_tag="ACHGNS_000848"
     CDS             27384..27743
                     /gene="rplT"
                     /locus_tag="ACHGNS_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFN6936812.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            27995..29332
                     /locus_tag="ACHGNS_000849"
     CDS             27995..29332
                     /locus_tag="ACHGNS_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFN6936813.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            29380..31509
                     /locus_tag="ACHGNS_000850"
     CDS             29380..31509
                     /locus_tag="ACHGNS_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFN6936814.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            31484..32173
                     /locus_tag="ACHGNS_000851"
     CDS             31484..32173
                     /locus_tag="ACHGNS_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936815.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(32581..33258)
                     /locus_tag="ACHGNS_000852"
     CDS             complement(32581..33258)
                     /locus_tag="ACHGNS_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN6936816.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTRFRWLR
                     LTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDYFED
                     IQDRPIK"
     gene            complement(33394..33588)
                     /locus_tag="ACHGNS_000853"
     CDS             complement(33394..33588)
                     /locus_tag="ACHGNS_000853"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936817.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(33638..34078)
                     /locus_tag="ACHGNS_000854"
     CDS             complement(33638..34078)
                     /locus_tag="ACHGNS_000854"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFN6936818.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(34082..34927)
                     /locus_tag="ACHGNS_000855"
     CDS             complement(34082..34927)
                     /locus_tag="ACHGNS_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFN6936819.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            35076..36494
                     /gene="pepV"
                     /locus_tag="ACHGNS_000856"
     CDS             35076..36494
                     /gene="pepV"
                     /locus_tag="ACHGNS_000856"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFN6936820.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(36551..37498)
                     /locus_tag="ACHGNS_000857"
     CDS             complement(36551..37498)
                     /locus_tag="ACHGNS_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN6936821.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(37721..38071)
                     /locus_tag="ACHGNS_000858"
     CDS             complement(37721..38071)
                     /locus_tag="ACHGNS_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFN6936822.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            38271..39350
                     /gene="pepA"
                     /locus_tag="ACHGNS_000859"
     CDS             38271..39350
                     /gene="pepA"
                     /locus_tag="ACHGNS_000859"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFN6936823.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            39494..39814
                     /locus_tag="ACHGNS_000860"
     CDS             39494..39814
                     /locus_tag="ACHGNS_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFN6936824.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            39836..40300
                     /locus_tag="ACHGNS_000861"
     CDS             39836..40300
                     /locus_tag="ACHGNS_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFN6936825.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            40505..41110
                     /gene="ytpR"
                     /locus_tag="ACHGNS_000862"
     CDS             40505..41110
                     /gene="ytpR"
                     /locus_tag="ACHGNS_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFN6936826.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(41149..42438)
                     /locus_tag="ACHGNS_000863"
     CDS             complement(41149..42438)
                     /locus_tag="ACHGNS_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFN6936827.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            42866..43345
                     /gene="rlmH"
                     /locus_tag="ACHGNS_000864"
     CDS             42866..43345
                     /gene="rlmH"
                     /locus_tag="ACHGNS_000864"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFN6936828.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            43548..43673
                     /locus_tag="ACHGNS_000865"
     CDS             43548..43673
                     /locus_tag="ACHGNS_000865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936829.1"
                     /translation="MIDEKKCLKNLLEDMDDIEENYLNGLGKQIYFRYLSYLKMK"
     gene            43670..44098
                     /locus_tag="ACHGNS_000866"
     CDS             43670..44098
                     /locus_tag="ACHGNS_000866"
                     /inference="COORDINATES: protein motif:HMM:NF025639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFN6936830.1"
                     /translation="MRHSNFLAFLFAPNETHNLSDEFFKMFLKRYIDSNDDTKAAINY
                     FDALLNSYEDLIVYRENKNIDILLVSEKNKIVVCIENKILSSESGGQLNKYQIYIERN
                     YSNYKKIFVFLTPDGNEPTNPSWGIITYKNIVEESVKYFV"
     gene            44169..>44988
                     /locus_tag="ACHGNS_000867"
     CDS             44169..>44988
                     /locus_tag="ACHGNS_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HFN6936831.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDF"
BASE COUNT        14302 a         8139 c         9585 g        12962 t
ORIGIN      
        1 caaataacca tgacccatta ggaggataat gatggctaaa gtaatagaaa aagaaaaaac
       61 gaagacaatt gacgtacaag caatgattga tgatttggca gaaaaagcaa atgcagcatt
      121 gaaagaaatg gaaagctttg atcaagaaaa agtagaccat atcgtgcatg aaatggcaat
      181 ggcagctttg gatcaacata tgccattagc gaagatggct gtagaggaaa caggccgcgg
      241 tgtttacgaa gataaagcaa tcaaaaatat gtatgcgtcc gaatatattt ggcataatat
      301 caaacatgat aaaacagttg gtgtgatcaa tgaagatgtc caaaaagggt tgattgaaat
      361 tgctgaacca gtcggtgtgg tttgcggagt aactccaaca acgaatccga cttccacaac
      421 gattttcaaa tcaatgatcg cgttgaaaac aagaaatcca atcgtttttg ctttccatcc
      481 aagtgcccaa aaatcttctg cagaagcagc acgagtggta cgtgatgctg ctatcgctgc
      541 cggagctcct gaaaattgta tccaatggat cgaacatcct tcgatcgaag cgacttctat
      601 gctaatgaac catccaggta tcgcgatcgt cttagcgaca ggtggagctg gaatggtcaa
      661 gtctgcttac tctacaggaa aaccagcttt aggtgtaggt ccaggtaacg taccagctta
      721 tattgaaaaa acagcaaaaa ttaaacgtgc agtgaacgat ttgatcgttt cgaaaacatt
      781 tgataatgga atgatttgtg cttctgaaca agctgtgatc gtggataaag aaatctatgc
      841 agcagtaaaa gcagaattcc aagcacacca agtttatatc gtaaaaccag atgaattgca
      901 aaaattagaa gatgcagtga tgaacgaagg aaaatatgcg gtcaatccat caatcgttgg
      961 acattctgcg atggaaatcg caaaattggc aggaatcagt gtaccaaaag gaacgaaaat
     1021 gctgattgct gaattggaag gcgtaggccc agattatccg ctatcaagag aaaaactttc
     1081 tccagttctt gccatgatca aagctaacaa cacggaccat gcgtttgatt tgtgtgaagg
     1141 tatgttgaat cttggaggat taggacatac agcagttatc cattcagaaa acgaagagtt
     1201 gcatgtcaaa ttcggtcttc gaatgaaagc ttgtcgtatt ttagtgaata caccatctgc
     1261 agaaggcggc atcggtgata tctataacga aatgattccg tcattgactt tgggatgcgg
     1321 ttcttatggt aagaactctg tttctcgtaa cgtctctgct gtaaacttaa tcaatgtcaa
     1381 aactgtagcg aaacggagaa ataacatgca atggttcaaa ttaccgccaa aaatcttctt
     1441 tgaaaagaac tctctattgt acttagagaa aatggaaaac gttgaacgtg tcatgatcgt
     1501 ttgtgaccct ggtatggtcc aattcggtta ctgcgataca gttcgtgaag tattggcacg
     1561 acgtaaaaat gatgtgaaga tcgaagtctt ctcagatgtt gaaccgaacc catcgacaaa
     1621 taccgtatac gcaggaacga aactgatggc agacttcaag ccagatacag taatcgctct
     1681 tggaggcggt tctgcaatgg acgcagctaa gggaatgtgg atgttctatg aacacccaga
     1741 cacatctttc tttggtgcaa aacaaaaatt cttggatatc cgtaaacgga catataagat
     1801 cgacaaacca gaaaaaacac aattcgtatg tatcccgaca acatcaggga ctggttctga
     1861 agtaacgcca tttgcggtca tcactgacag tgaaacacat gtgaaatatc cgttagcaga
     1921 ttatgcattg acgccagatg tcgcaatcgt cgatcctcaa ttcgtcatgt cagttccagc
     1981 atctgtcaca gcagataccg ggatggatgt gttaacacat gcaatcgaat cctatgtttc
     2041 agtcatggct tcagattata cacgtggatt gagcctacaa gcaatcaagt tagtctttga
     2101 ctatttagaa aaatcagtaa aaacacctga tatggaatca cgtgaaaaaa tgcacaacgc
     2161 ttctacaatg gctggtatgg cgtttgctaa cgcattccta ggtatttgtc actcagtcgc
     2221 acataaaatc ggcggggaat acgggatccc tcatggacgg acaaatgcga ttcttttacc
     2281 gcatattatc cgctataatg caaaagatcc acaaaaacat gcgatgttcc ctaaatatga
     2341 ctatttccgt gcagatacag attacgcaga tatcgcaaaa ttcttaggtc tgaaaggcga
     2401 aacaacagag gaattagtcg aagctttagc aacggctgtt tatgaattag gaaaatctgt
     2461 cggcatcaac atgagtctaa aagctcaagg agtcacacaa gaaacactag atacaaccgt
     2521 agaccgtatg gctgaattag cttacgaaga ccaatgtacg acagctaatc cgaaagaacc
     2581 attgatctct gaattgaaac agatcatcat tgatgcttac aacggataag caaaagaaag
     2641 cgtagaaaaa agataagaaa atagatgaaa aagaacttgg aaaacgttgt ttctccaagt
     2701 tcttttttta atttccaagt aactgaaagt tacttttgtt atttaacatt taggtcaatt
     2761 ttagacagtt tttgttcaaa aagcgatatt tatagtggac ggtattactg ctatattaag
     2821 gatataaaat gaaagcgttt aaaaaggtgg cgtaaagatg attattaaag aagagatttt
     2881 tgtacctaaa gaattagtga aagaaaaaat tgacctgctc gttgaaaacc tgacaacgat
     2941 caaagacgat aatggtgaat ttttgttgga ctttgatgga ctgaaagtag atgacaaaag
     3001 ctggacagtg tggaattggc cacaaggtgt cggtctatat ggtatttata aaaattacca
     3061 gatgacaaaa aatcctcgcg cttatcaagt ggtcaatgaa tggtttgagg atcgtatgga
     3121 agaaggcgct ccgcccaaaa acgtcaatac aatggcaccg ctattgacta tggcttactt
     3181 gtatgaagac acgaaagatt cacgctattt gccttattta gaacaatggg cagaatgggt
     3241 catgaatgac atgcctcgga caaatgaaga cgggttacag catgccacat atggaccgga
     3301 aaataagaat cagttgtggg acgatacatt gatgatgaca gtgcttcctt tggcaaaaat
     3361 cgggaaactg ttgaatcgcc cagaatattt agaagaagct cgttaccagt ttatgatcca
     3421 tatcaaatac ttgatggata aacgaacagg attgtggtat cacggctgga cgtttgaagg
     3481 aaatcataac tatgccgaag cattttgggc tcgtggaaac tgctggttga cgattgctat
     3541 tccagagatc attgaaatct tagaattatc gaaagaagat gcattgcgca aattattgat
     3601 cgaaacactt gaagcacaag tgcgtgcatt aaaaacttat cagtctgaaa gcggtttatg
     3661 gcatacgttg ttggatgatc cttcttctta cgtagaatct tctgcaactg caggatttgc
     3721 ttacggtatt ttgaaagcgg tccataaacg ttatttacca caagaatata aagaagtcgc
     3781 ttatcgtgct atccaaggat tgcttgaaca aatcgatgaa aaaggagaag ttcaaaacgt
     3841 ttcaatcggg acaggaatgg gggacagttt agatttttat cggaatatcg gaatcacagc
     3901 aatgccttat ggacagtcgt taacggtgct ttgtttgaca gaattgctcg tctcttattg
     3961 ttaaaagaaa ggaaacccat atgaaaaaaa catcactgct gtttgcagca agttcgctta
     4021 gcctaggtct gctaggtcta agcggctgcg gaaataattc tagtgtggca actgtcgcag
     4081 atgcagatgt aaatgagata gttaccttgc gctttgctta tgcaagcaac agccagccag
     4141 taattgatgc aatgaatgaa tttgggcgtt tagtaaaaga aaaaacagat ggttccgtac
     4201 agatccagta tttcccagat ggacaactgg gtggagaacg tgaactgatc gaattgacac
     4261 agtcaggagc agtagatttc acaaaagtaa gtgcctcagc gttggaaagt ttctctaaag
     4321 attattcgat tttttcagtt ccttatctat tcaatgatga agaacatttc tttaaggtga
     4381 tggataatga agaaataatg gagccaatct accaatcgac caaaaattta ggatttaccg
     4441 gattgaccta ttatgattct ggtcagcgca gcttttatat ggtagacggt ccgatccata
     4501 cgccggaaga tttgaaggga aagaagatcc gtgtgatgca aagcgaaacc gctatcaaaa
     4561 tggtagagct tttaggcggt tcgcccgtac ctatgggaag tgatgaagtg tacacgtctc
     4621 ttcaatccaa tttgatcaac ggatcagaaa acaatgaatt tgtcttgtat acagctggac
     4681 atggtggtgt ggctaaatac tattcctatg atgagcatac acgtgtgcca gatatcatca
     4741 tcatgaatga tgccatcaaa gaacagctaa ctgatgagca ggaaaaagcc attgaagaag
     4801 cagcgaaaga atcaacggtt tttgaaattg aagcttttgc aaaagcaatt gaagaagaaa
     4861 aagctttagc acaagaagaa catggagtcc aattcaatga tgtagataat acaccgtttc
     4921 gagaggcagt tgccccactt catgatatat tcaaaaacga tcctacctac aaagaccttt
     4981 atcaaaagat ccaagagcaa ggagcgtagc tgtgatgaaa atgattttaa acaaggtatt
     5041 agagatcctg ggagctgttt tgcttgttgg gatggtcgct gttgttttat ggcaagtatt
     5101 ttccagaacg gttttaggaa atccaaatac ggtcacggaa gagcttgttc gattcggtct
     5161 tgtctggttt gccatgttgt cttccgcgta tgttgtggga caaaaagcac atttagccgt
     5221 aacggtttta agtgaaaaat taactggcaa aaaaagtaaa attttagaag ttgttgttca
     5281 aagtttattt ctggtatttg ctgcgatgat catggtgtat ggcggatgga atgctgtcac
     5341 attgacaatg gggcagatct cgccttcttt aagtattcct atgggatatg tttatctgtc
     5401 agtacctgta tcgggggtac tcatcattat ctatagcctg atcaatctaa tcgatacatt
     5461 aagcaacaag ccagcaattc aagaaaaaat ataaaaatgg aggaataaaa tggcaataga
     5521 agccggatta gtgttatttt ttgtatttgt tgtgttgctt gtattaggga tgccgattgc
     5581 tataagtatt gcattctcat cgatgatgac tttattgtta gttataccgt ttgatgtttc
     5641 tgcttttagt tcagctcaaa aaatggtggg aagtatcaat agtttttcgc tgatcgctat
     5701 tccgtttttc gttctctcgg gcattatcat gaataatgga gggatagcta agaaactagt
     5761 ggattttgcg atgttgttcg tcgggcgcgt gccgggggct ttggctcata cgaatgtgtt
     5821 agggaatgct ttatttggtt ccatctctag ctcggccatt gccgcttcta ccgcgattgg
     5881 cggagtgttg atccctcagc aagtggaaga aggatatgac cggaagtttg cgacagcggt
     5941 caatatcgcg tcagctccga caggtatggt cattcctcca agtactgctt ttatcatgta
     6001 ttcacttgtt gctggtggtg cttcgatctc tgctttgttt atgggaggtt atctcgtagg
     6061 cgctttgtgg gcgctaggaa tcatgctagt agcattcatc gttgctaaaa agaataaata
     6121 ccctgttgtc gaaaaaggac agatggctca tgctggaaaa ataataaaag aggcgattcc
     6181 tagccttcta ttgattgtca tcattatcgg tggtatcttg acggggatat ttacagcagt
     6241 agaagcttca gcgatagctg tcatttactc cctgttgatt gctatgtttt actataaaac
     6301 ggtaaaacta aaagatatac ctaatatgtt gtttcaatca gtagcaatgt cagggacgat
     6361 catgttctta ttggctactt cttccatgat gtctttcgcc atggcgttta ccggtattcc
     6421 tcaggcaatt agttcgttga ttatgggcgt aacggataat aaatttatta ttctgttatt
     6481 agtcaatctt gtgttattac tggtgggtat gtttatggat gtggcgcctg ctatcttgat
     6541 ttttacacca atatttttac caatcgtcac aagtgtaggg gttgatcctg tacattacgg
     6601 gttgttttca atcatgaatc tgtgtgtagg ctccatcacg ccgccagtag gtacaggact
     6661 atatgtcggt gcaagtgtag gaggcgtaaa agcagaggca atgctgaaac cgcttcttcc
     6721 tttttatggg gttatcttag tcgtcttgtt tttgattact tacttcccgc aaatcgtcat
     6781 gtggctaccc aatatgctga gttaggagaa tagttatgtt gaacattaca attagaggac
     6841 atgatctttc cgaagtccaa acgatagaag atcttgcaga aaaaacaaaa gaacaaggga
     6901 ttcatacact gcagctcgcc ttaggtcttt cgtttcctga aataccttct ggcgcaaatg
     6961 agatcaattc tgggatggga aattatgtca aacgagtttt agaaaagcaa gaagtgaccg
     7021 taggaatttt aagttgctat atcaatatga ttcatccaga tttaacgata cgacaagagt
     7081 tgctgacaaa gttcgagcag tatcttcgct atgcttcttc ctttggtgcg tcgatggttg
     7141 cttcggaaac tggatgtgtt ttgccggaaa tccaatatac ggaagaaaat ttcacagatg
     7201 aagcatttgc tgaagctgtg tccgttatcc gtcgcttggt aaaagctgga gaaaaatacc
     7261 aaatgatggt ggggatagaa ccaggtttga atcatccggt gtattcttta gcaagggtag
     7321 agcagctgat ccaagcagta gattcggact atctaggaat cattttagat ccgaccaatt
     7381 tgatcacaag tagtgattat caagaacaag tccaattggt agaagaagct tttgaacgtt
     7441 ttggtgaaaa gatatgtgcg gttcatttga aagattttcg tgtcgaacag gaaaaaatcg
     7501 tgccggtaaa tttgggagac ggtgtgatcg agtacacaaa aatcaaagaa atcatcatga
     7561 agaatcgtcc gtacctgtat gtggttcttg aagaaacaaa agatgatggg attcgttatg
     7621 gaagaagtct tcttgaataa tgtaaagaag acgttggtta actaatattt agaatggagg
     7681 gggcggggct agaagatgcc acgtctcttc ttttttataa gagtatataa aagcagctct
     7741 ttttgattac aaaaaacgtg gtataataaa attgtgcata agtgaacaaa aggaggtgcg
     7801 gcacatgctc caaaagaaaa tcagcggtga acaagtcaat acgatggttt ctgatcagta
     7861 tgaaatctat catgtcaaag atgtcgtcag ccaaaataaa acgatctatc atcatcatga
     7921 tttctatgag atccatgcta cattaaaggg agaagcgact ttttatttga acggacgaca
     7981 atttacgatc aaagcaggaa gtgtgctgct catccattcc aatgatttgc atcgtatcgt
     8041 ccgccaatct tcagatgtat tcgagcgtgt ctatatcttt gtcacccctt cttttctaga
     8101 aagtcgttcc acgccatggt ctaatctatc ggcttgtttt caaccaatgg gagagaagag
     8161 gagtcgtgta ttgcaaatag aacaggaagt attgaagcaa aaattatcat ttatcgacca
     8221 tcctcctgat aaaaaaattt acggtgaaga cataaaatat gaacaagctc tggtagaata
     8281 cttgattttt ctcaatcaag ttgtacaaaa agaagagagt gcgcaagaac agaatgctgt
     8341 agttcctaat gagcgtgtgg aacaaatgtt acaatatatc tcaaaaaatt tatctcatcc
     8401 gctttcttta gaggaaatgg aaaagcagtt ttttgtcagc aaataccata tcacacgaga
     8461 atttaaaaag tacactggat ttacgtttca tcagtatgta atgaagaaaa agctgatcta
     8521 tgccaagcac ttgttgcgag aataccgcag ctctagtgct gtatatagta aaagtggttt
     8581 ttcttcttat ccgcattttc tgaaagcatt caagagagag ttcggcataa cgcctaaaga
     8641 atttttgaaa cgtgatacac agaaacagtt ggttcattat gaacattttg aagatgaaca
     8701 atgagaatga tcactaaaaa ataaccgcag tagctgctgt tgtttacata gaagcagaaa
     8761 ctgcggttat tttattttta tctatatatt tcaaaatatt ttgaaaaaat gacgtacata
     8821 ttatatgaat cagctgaatt tacgaaagtc tctcggcgat agtcctgttt ttgctttgaa
     8881 caggcggcta aagtaagcaa aattctcaat ccctatttct tcggaaatca ttcgaaccgt
     8941 aaattctgta taccgaagaa ggtctttagc gtaagaaagt tttttgctga tcaaataatc
     9001 gattggtgga gaaccgataa atttggaaaa atctttgctc agctgatatt tattcaataa
     9061 gaattgttgt tctaattgat caagtgtaat cgtttctttg aaatgttcgt ctaagtattg
     9121 cttcagtttg taaacgtagg cgggaatatc ctcttcttcg aaatctagct gatacttttg
     9181 caaaatcagt tcattcagca actcgtggat agctacagag gactggaaat cggtacgggc
     9241 attcgtgcct tgttgcatgt ggatcaattc tgtgatcaaa tggtggatct tgttttctga
     9301 tggatctcct gttgaatgga aggtgttatg gccgtctttt gtgaactcct gataaaatga
     9361 ttggacgttc tgaccattca aatggatcca atccatttcc catggttctt cgctgatcgt
     9421 ttgataacgc tgatattctt ggcagtcaat aaaaaataag tctccagctt ttagctgttg
     9481 ctgattatca ttatatgtaa gcgaaccttt gccagacaaa gtaaacttta tcaagtaaga
     9541 aggtaaatgt gctcgttcag tatagtaagg gattggtgct ttgaagtaac cgatttcttg
     9601 gatataaaaa aataaattcc tgcttctttg tgtaggcgtc aagatcactc tttttgagtc
     9661 ttccgaccat gagtggttgg ctttttccca gtaatccatg taaacactcc caacttgtca
     9721 agataatgca attatatacc aaaatagtgt gaagcaaaat ataagattct gtattatgat
     9781 agcattgtca ataaatgata acgctttatt aataaagtgc taatcaacaa taaagtaaaa
     9841 gaggtggaga aatgaatcca acatttgttg ccatcgatat cggtgcatca agcggacgac
     9901 taatgaaaag tacgagggat gcaaaaggtt ctttgatctt ggaagaagta caccggttca
     9961 aaaacggctt tcggaacaaa gacggtcacg atcgctggaa gatcgattat cttatcaaag
    10021 agattttgat tggatttgaa aaattaaagc agacagggat tgatcgcctt tttgcgggga
    10081 tcgatacatg ggcagtcgat tactgtctga ttgatgaaca aggggatcgg ataaatgatc
    10141 cgattgctta tcgggacaag cgtaccaatg gagcagtaga acagttttct gaaatctatt
    10201 ctctagataa gttgtatgag cagacaggga ttcagatcca gcctttcaat accttgttcc
    10261 aattgtttgc tgaagaaaaa gaaaatttgg caaaagcaca taaattgtta ctgatacctg
    10321 attatttagg gtatgtcttt actggtaaca tggtgacgga gaaaactaat gcatctacta
    10381 tgcagctgct gaatgcaggg acaaaagaat gggaaataga acttcttgaa aaaatcggtg
    10441 tctcgaagga attatttcca ccattgacag agccgggaac gatactagga gatctgcaaa
    10501 aagaacaatt ttcaaaatat gatttgccag atgtgacctt tatcacagta gcaagtcatg
    10561 atacggcgtc agctgtatta ggaacgccag gtcaaggaga acgttggggt tatatcagca
    10621 gtgggacatg gtcgctttta gggattgaaa caaagatcgc caatgtttct cgcaatgctt
    10681 tttctgaaaa ttatacgaat gaatggggtg cccataacac ggtgcgtttt ttgaaaaaca
    10741 tcatggggat gtggttgatc caggaagttg cccgaatgca agagtacgag tacagttatg
    10801 cggaattggc agagcttgct gcaaaagaaa aaccgctgca gcaagtgatg gaaatcaatg
    10861 accctcgatt tttgaatccc ccaaatatga tagaagagat tcagcaatac tgttttgaaa
    10921 cagagcagcc agtccctgtc tcatcgggag ctatcgctcg ttgtgtgtat gataatcttg
    10981 ctctttgtta cgcacaagag ctgatcaaag tagaacactt aactggaacg aaattggaaa
    11041 agctgcacat cgtagggggc ggatcgaata atcgtctact gaatcaattg acagcagatt
    11101 atgccggaat cattgtagaa gcaggaccta gtgaagccac agcaatcggg aatgttgcca
    11161 tgcaaatgct cagtacaggt cagttttcga atatagaaca agcaagaaat gtaatcaaac
    11221 agtcgtttcc atgtgaaact ttttacccag atgaaaataa gcaaaacacg atggaatggt
    11281 acgtgaataa aatcaaagga gtaatcgtat gaacacgatt gaaagaaatt acgaacaagc
    11341 aaaagaaaaa tatgcaacta tcggtgtaga tacagatgct gtactagaaa agatacagga
    11401 catcaaaatt tctatgcatt gctggcaagg agatgatgtc aaaggtttct tgacaccgga
    11461 tggcgaattg accggtggaa tcatggcgac agggaatttt ccaggagccg cacgaacacc
    11521 tgaagagctt agacaagatc tggagaaggc ttattcgttg ataccgggca aacacaaatt
    11581 gaaccttcat gctatctatt tggatacaga agaatctgtc gatctaaatg aaattgagcc
    11641 gaaacatttt gaaaaatggg tagagtgggc aaaaaaagaa gaaatcggtc ttgacttcaa
    11701 tccgactttc ttttcgcatc caatgatgaa agacggcttt acattggctc atccagataa
    11761 agaagttcgt gaatactgga tcgaacacgg gaaaagaagc cgcaaaatcg cggagtactt
    11821 tgggaaagaa actgggcaaa caagtatcaa taacttttgg gtacctgatg ggatgaaaga
    11881 taatccgatt gatcgtttta ccccacggga acgtttaatg gaatcactcg atgaaatttt
    11941 tacagaagag ttagatgaac ggtatgtaca agatgcagta gaaagcaaac tatttggttt
    12001 aggcgctgaa gcttatacag tcggttccca tgaattttat atgggttacg ggttgactcg
    12061 tgacaaactt gtttgtctag atgctggaca ttttcatcca acagaagtga tttccaataa
    12121 attatcttca ttagctttat tcagcaaagg aatcatgttg catgtttccc gtccagtgcg
    12181 ttgggattca gaccatgtgg ttttgatgga tgatgaattg caggaaatcg ctaaagaatt
    12241 agtccgtaac gaattgttgg aaaaaacaaa tatcggtctg gatttctttg atgcaacgat
    12301 caaccgtatc gctgcttggg tcatcggtac gagaaataca caaaaagcac tgctgaaagc
    12361 tatgctggaa ccagttgagc gattgaaaga aatggagctg gcttttgact ttacttcaag
    12421 aatggcttat acggaagaat tgaaagattt cccgtatgcc gatgtatgga attatttctg
    12481 ttacaaaaat caagttccag ttggtttgga ttggctggaa gaagtccagg aatatcaaaa
    12541 agacgtgttg gaattaagaa aataggagtg tggctatatt gaaaaaaata gatattttac
    12601 aagcgcctta tgtgaaagaa atgatcgaaa cgacgtcgaa tctttatcgc ttgggatggg
    12661 atgaaagaaa tggcgggaat atttcttatt tactaaaaga agaagagatc acaccgttct
    12721 tggatcctga aaaagtgatc cgccgcatct cgatgatttt tgatgccagc cgtttagcag
    12781 gatgctattt tattgtaact ggttcaggaa gatattttaa aaatgtagcg agcgaacctg
    12841 ccgagaatct tggaatcgtt cgagttgctg aagatggcaa aacactggaa cttttgtggg
    12901 ggctggaaaa cgaatgcctt cctacaagcg agttgccaag tcatttcatg agtcatatcg
    12961 cccgtttaga agtagaccca gataatcgga tcgttatgca ttgccatgct agtcatctat
    13021 tagcgatgag ttttacgcat gaattagacg aacgttcctt tagtcgtacg ctatggcaaa
    13081 tgtgtacgga atgcttagtt gtttttccag aaggaatttc catcattccg tggatggttc
    13141 cgggaacaaa tgagattggg gaagcaacag ctgaaaaaat gaaagaaact cgattggttt
    13201 tatggccaca acatggaatc tacgggtcag gaaaagacat ggatgaagtc tttggtttga
    13261 tcgagacagc agaaaaggca gcagaagtct atacgtacgt caaagcgcaa ggaccaatct
    13321 tgcaaacaat cacggacgaa aacctgtggc atttagcaga tgcgtttggt gtcactccaa
    13381 aagcaggtta tttggaggaa gttcatacga aagcaggtgt ctgacatggt gaaaaaagct
    13441 ttttgcatga aagtttaccc agaaaaacaa gaagaataca aaaaaagaca tgatgaactt
    13501 tggccagaaa tgcgtacagc acttcgtgaa cacggggtca tcgactattc gatctatctt
    13561 gatccgagta caagtttgtt atttggttac ttagtgatcg aagatgaaaa gttatgggaa
    13621 aaaatggcaa atacatctat caatcaaaaa tggtggaagt acatggaaga tatcatggag
    13681 acaaatccag atgcctctcc agtcagttgg gaattgatgc cagtctttga actgtagcaa
    13741 caagcaactg ggcaaccggt tgcttggggt tctttcgttt ttattgtgaa aaataataca
    13801 aattaggagg aatagaaaat gagtaatcga atgattttaa atgaaacatc gtattttgga
    13861 aaaggagcga ttgagtcgat cgtccctgaa tttcagcagc gaggattcgg caaagcagct
    13921 gtcattactg acaaaggatt gatcgagcac ggtatagcaa caaaagtaac agaacttcta
    13981 gataaagcag caatcgctta tgctttatac gacgggatcg tacctaatcc aacgatccaa
    14041 aatgtaaaag atggagtggc ttttgttaaa gaggcagagg cagactgttt gatcgcaatc
    14101 ggtggaggtt cgccaatcga tacggcaaaa gccattggga ttattttgac gaatccagag
    14161 ttttcagacg tcattagttt agaaggtgtt gctgatacga aaaatccatg tttacctata
    14221 ttagctatac caacgacttc tggaacagca gcagaagtaa cgatcaacta cgtcatcaca
    14281 gatgaagtaa atcaccgaaa atttgtttgt gttgatcctc atgatatccc aatcgtcgcg
    14341 ttcatcgaca gcgatatgat gatgggaatg ccgaaaaaat tggctgcatc aacaggtatg
    14401 gatgctatga cgcatgctat tgaagggttt atcacaaaag gcgcttggga aatgactgat
    14461 atgctgcatt taaaagcaat cgaaatcatc ggccattctt tggaagcttc tgtagatggc
    14521 gatcaaaatg ggcgagaaaa aatggcatta ggacagtata ttgcgggaat gggcttctca
    14581 aatgtcggtt taggactggt acacgggatg gctcacccgt taagtgcatg gtacaacatt
    14641 cctcatggcg tagcgtgtgc agcacttttg ccaactgtca tgaaatataa caaagaatat
    14701 actggagaaa agtaccgtga gatcgctctt gttctaggga ttaaaggagc agctgaaatg
    14761 tctttagaag atgtaagaga agcggcttgt ggtgagatcg atcggttgag caaagcggtt
    14821 ggaatcccag aaaccatttc tgaattaggt gtaaaagaag cagatattcc agcaattgca
    14881 gaagatgctt tgcgagatgt atgcacacca ggaaatccaa gagaaacgac agtagaagaa
    14941 atcattgctt tatatcaaag tttgatgtaa aaacgaagcg gatcttcaac attaaattgc
    15001 ctgtaaagag aaatggtagt ttatatctgg atatctcttt taaattgatg gtatttccgc
    15061 tataattttt cttataaaga aataggagaa tattatatgg aaaataaaaa gccttatgga
    15121 actgtattga tcaaagcctc taaaatacta gactatttag cggaaaacca agatgtctct
    15181 ttgcagacga tcgcaaaggg tgtgggaatg acctcttcta cagtcttgaa aatactagat
    15241 actttgttga tgatcggcta cgtaaataaa aattcagaga aaaactatcg tttaggtgca
    15301 aagcttattc gctacgcgaa taaaaacatt gaacaaatcg atttggtaga gttgacttta
    15361 ccgtttttag aacagctgca gcaaaagatc gatgagacta tccacttagg ggtattggac
    15421 aacaatgaga tcttgtacgt gaacaaacta gatccaaaac atcaaacgat ccgcatgtct
    15481 tcaaaaatcg gaatcacccg accattatac aattctgcga tgggcaaagc cgtattagca
    15541 gaattcagtg aagagcagta cactgattat cttgaaaagc atccgttgat cccttacaca
    15601 gaatatacga tcaccaatgc attacggttg aaaaaggaaa tcaaaacggt aaaagaaacc
    15661 cacatcgcat ttgacgatga agaaatcgaa cgtgatattt tttgtatagg agcctcaatc
    15721 gtgaaaaacg aacaaatcat tggtgcattt agtgtaagta tgccgaaata tcgtttaaca
    15781 gatgagacaa aagaacaaat aatcagtgcc ctcaagcaaa caaaaagcga aatcgagcaa
    15841 agaattcaaa aataatgaaa aaagcgtttg caatttttct tttctgccgt ataatatggg
    15901 tatagatttc caagttataa aataaaattt ctaaatagga aataggtgga gaagatgaaa
    15961 aaaattgata ttttatctcg attgaaaaac gccggagtga ttgcggtggt acgcggaaaa
    16021 agcaaagaag aagctttgaa tgcttgccac gcaatcatca aaggtggtct aactggtatt
    16081 gagttgactt ttacagtccc tcaagcagat caagtaatca aagaactttt gtcattctac
    16141 aaagaccaac cagaaatagt gattggcgca ggcacagtat tagacgctgt cacagctcgc
    16201 ttggctatct tagctggcgc agagtatatt gttagccctt cctttgatca agaaacggca
    16261 gagatgtgca atctgtatca aattccttac ttaccaggat gtatgactat taccgagatc
    16321 aaaacagcgc tgaaaagcgg ggtagatatc gtcaaactat ttcctggaag cgcttatgga
    16381 ccaagtatca tttctgcctt caaggcgccg atgcctcaag tcaatatcat gccaactggc
    16441 ggtgtgagtc tggacaatat gaaagagtgg ttcgatgctg gtgtcgtaac agtcggagta
    16501 ggtgggaacc ttttagcacc cgcagctaca ggagattttg acaaagtaac ggaagtcgcg
    16561 caacagtatg cagcaaaaat gaaagaaatc aagaggtagc tgcttatggg aaaagtagtg
    16621 acactcggag aaatcatgtt gcgattgtcc acagagcagg gaacccgtat cgattcaagt
    16681 gaacgattcg ctgcccatta tggcggaggg gaagcaaacg tagcaatttc tttagctaat
    16741 tacggacatg aagcgatttt tgctacaaaa gtacctgata acgcattggg aaaagcagtc
    16801 aaaaagcact tgcaatgtta tggagtagat acgaaacagg tattatctgg tggatcaaga
    16861 ctaggtactt attatatgga aactggtagt ggcgaacgcg ccgcctcagt gatctatgat
    16921 cgtgccggat caagttttgc tgaaatggat catttggaat ggtcgctaga ggagttattc
    16981 caaggaatag atatatttca tgtttcagga atcaccccag ctttgtctaa aaaatggcaa
    17041 aaaatgacga aacaggtgat agaagctgca aaaaaagctg gctgcctgat cagcttcgat
    17101 atcaactatc gtggaaaatt atggacacag caagaagcca gcgaagtaat ccatcagctt
    17161 ttacctttag tagatatctg ctcagctgga aaaatggatg ctctttattt gttaggtatc
    17221 gagaaagcgc cagaagcaga agaacaacct ctaatttatt attatcaaaa aatgcaggaa
    17281 aagttcccga atattcaggt gttttattcg accaagcgta ccgttcattc agcaagtgaa
    17341 aatgaattga aggggacatt gtggatgaac catgaatatg tggaatccca agtccataga
    17401 atcaccccaa tcgtggatcg agtaggtggc ggagatgctt ttgcaggtgg cgtgctccat
    17461 gggattttat ccaatatgtc tccgcaagaa atcattgatt ttgctacggc agcttctgcc
    17521 ttgaagcata cgatccatgg ggactgcaac caatttagtc aagaagaagt cgcacaattt
    17581 ttagcatgcg gctctggaaa aatcattcga tagaaggagt agaagaaaat gcaagaaatg
    17641 gaaacacgtt atacacacag tccagaagat atccgtcatt attcaacgga acaattgaga
    17701 aaagaatttt tagtagaaaa ggtatttgaa ccagggaaaa tcagtttgac ttatacgcac
    17761 aacgatcgaa tgatctttgg tggagtgaca cctactgaaa aagaattaga aatcacatta
    17821 gacaaagaac taggtgttga ttacttctta gagcgtcgtg aactaggtgt gatcaatatc
    17881 ggtggaccag gattcatcga gatcgatggg caaaaagaag aaatgaaaaa acaagacgga
    17941 tactatatcg gaaaagaaac acgacatgtt gtattttctt ctcaagatgc tgaaaaccca
    18001 gctaaattct atattagttc tgttccagct catcataaat atccaaatgt aaaaatcagt
    18061 atcgaccaag taaaaccaat ggagactggt gaaggcctga cactgaatga acgtaaaatc
    18121 taccaataca tccatccaaa tgtatgcgaa agctgtcaat tacaaatggg ttatacgatt
    18181 ttagaaccag gaagcgcttg gaatacgatg ccttgtcata cacatgaacg tcgtatggaa
    18241 gcttatgtct actttgatta tgccaatgaa gatacacgtg tgttccacat gatgggcaaa
    18301 ccagatgaaa caaaacattt agttgtagac aatgaacaag ccattatttc gccaagctgg
    18361 tcgatccatt caggtgtagg tacaagcaat tactcattca tctgggcaat gtgcggagaa
    18421 aatatcacct atacagacat ggatatggtg caaatggatc aattaaaata agcataaggg
    18481 agagaaaatg atggaattta gtatggatat gtttcgttta gatggaaaag tagcattagt
    18541 caccggtgct gtctatggta ttggatttga gattgcacgc tctttagcta cagcaggagc
    18601 aaaaatcgta tttaataact tgaatcaaga atcagttgat gaaggtatag cgcattataa
    18661 agaagcaggc atcgatgcaa aaggttatgt ctgcgatgtg accgatgaag aagcggttca
    18721 agcgatggtt caacagatca aagaagatgt aggttcagtt gatattctag tcaacaatgc
    18781 agggatcatc aaacggacac cgatgatcga aatggatgcg gcagacttcc gccaagtaat
    18841 cgacgtggat ttaaatgccc cattcatcgt ttcaaaagca gttattccgg atatgatcga
    18901 aaaaggcggc ggtaaaatca tcaatatctg ctccatgatg agtgaattag gacgcgaaac
    18961 ggtcagtgct tatgctgcgg caaaaggcgg attaaaaatg ctgacgaaaa acatcgcttc
    19021 tgaatacggt caatacaata tccaatgtaa tggaattgga ccaggttata ttgctacacc
    19081 gcaaactgct ccattacgcg aaacacaaga aaacggtgaa cgtcatccat tcgatcaatt
    19141 tatcgttgga cgtacgccag cagcacgctg gggcgagcca atcgatttgg ctggaccatc
    19201 tgtcttcttg gcttctagcg catctgattt cgttaacggc catgtcttgt atgtggacgg
    19261 aggcatactt gcctatatcg gaaaacaacc ttaaaagagc tttagtacac tgtttagtgg
    19321 agaggttgtg aaatcagcgg attgaacgaa taccgaagga cagctgatta aacatcattt
    19381 attcgaaaaa agaggctggg acagaagcat ttaactccaa gaaataagaa ggaattcacg
    19441 aaaattgctt ttcaaatttt tgtgaatttc agcttatttc cgaaggagtt gcttttactt
    19501 ccgccgtttg tacgtgttta gagcgtgaga caaaagtgtt ctttactttt gtcccacgct
    19561 cttttctgtg cgtcttccat acttcactaa gtcgatccct cttgcttcgc accaatcctt
    19621 cactggtccg gaaaagatca gagattgttg gtgtagggcc gcggtattcg ttgcgccgac
    19681 catagtataa agcagacgaa gctcttcttg ccattgcttc atcagtataa tagtttcttc
    19741 tacgccgtga ttcatcagat gcgtcagtac ggttcctgaa gtacccacgg catttgcgcc
    19801 gagacaaagg gctttaaata tgtcataggc attgcggata cctccggagg ctaggatttc
    19861 catagaagtg tctgcttcat ttgcttcaag caatgaagag actgtcgatt gcccccagtc
    19921 agctaaataa ctcagttccc gcttggagcg acgggcgttt tcgatctggg tgaagcttgt
    19981 tccgcttcga ccgctgatat caactgtatg aactcctaaa gcagctagat cattgagcgt
    20041 ttctcttgtc atgccaaaac cgacttcttt tacgatgagg gggacgtcaa tggctgtttg
    20101 agtttcttga ataagtactt tccaattggt aaaatcacgg tctccctcag gcataaccaa
    20161 ctcttgaggt gcattcaaat gaatctgtaa ggcatctgcg tgaaacaatc ggatagcttc
    20221 tttggctctt tcgacagaag tccctgcacc gatgttggca atgattttcc cgtgaggata
    20281 ttcttgtcgc ataatcgtat aagtatcggc taaagaaggg actttcaagg cagcgctgac
    20341 tgaaccagtg gcaatcatca aatctgcttc tcttgcgacg attgcaagat cttggttgat
    20401 tttttttgtt ttttcgcttc cgccagtcat cgcattgata taaaatgggc tgcttagtgt
    20461 aaacccaact gcctgtgtac ttagatcgac atcagaaact gctatttgcg gcaaagggtt
    20521 atggatgatc cgtacgaagt caaattcgtt tttttgttta tcatgaaagg ctttagctag
    20581 tgaaacatgt tcgtcttttc gattcatcgg ttttcctttc tttgatcgct atagacatgt
    20641 aacggcagtg gagtgatttc tgctttttcc catgcactca ttaaaggaag aatgccagat
    20701 ttctggtcaa caattacgat tccgcaatcg cccccacctg caccagaaga ttttgcggct
    20761 ccttcatact gttcagctaa attacacaat ttgttcaaag caggcgtttc gatgacgacc
    20821 ccagtgattg cagataaatc atgcagtaat tgtcggtttt ttcgaatcat ctgttggatc
    20881 aacgttacat tattttcttt aaaccctttg atcatttcgt tgacacattc tgtactattt
    20941 tttaagaaaa gctgataagc caccatttta tcttctctcg aacggtgaac ttgatcgacc
    21001 aaatcagaag tagaggcagg gctacccgtc caaccaatca ataaacgtaa atcttcagga
    21061 gcaatcaatg gctcaatgga tagacctggc caatccaggg caagtaactc actgatagaa
    21121 tgctgagttt cttgttcttg gagccaagga tggtcgaagg ttgagaaagc gatccagcca
    21181 ccataacagc tagcagcgat gtcgccgcag gaaccattat cttgaactgc taaattggct
    21241 agtgctgcaa tcttgaaaat ctccaactga gataaattca acgcgtaaaa aacattcaag
    21301 gctttgaccg ttgcaacggt cacggcaccg ctagaaccca atccatattt ccggccattt
    21361 gaactgtcta actcacttgt tactttcaga tcataaaagg ataaaagaat gtttttttct
    21421 tgtgcatact tttcagtcaa gcgaatagca gcaaggatat aatgaaaagg attttcccga
    21481 atatctaaaa ctaattctcc gttgcgtctt gtccaacgta caggcatccc actatattgt
    21541 gcagattgga tacttccgac ttttcgtgca gattctacag tgactgtcac gaattgatcg
    21601 actgcagcga taactgctgg atggcctgtt tcaacaactg catattctcc ggcaatatag
    21661 agtttgcctg gtgcagatac ttcaatcata aaaatctccc tttattcaat aatcgcaatt
    21721 cctggaccag catgtgcagt aattacttgt tctttgctaa ataatttggt aaatgtttct
    21781 tgtacctcag ataaatggct gttttccact aaaactttga cattagggcc agcatccatc
    21841 gtaaagtagc atggaatgcc ttgctttctc aattgtcgca ccgcatccat cgcttttaag
    21901 ctgtcaggag accagtaagt gaatggaggc tgggcagcta aagtcgttcc atgcattttt
    21961 agtccgtttc tttccatcgt ttctccgaga agttggaaat cttttgtttt gattgcttgt
    22021 ttcaattgat atagatcccc ttcaacggaa tctaaccagc cttgataaaa actggatgtt
    22081 tcgactgtcc gacgcatccc atttctgctg gacacttctt ttttctggtc gttgatcaaa
    22141 acgaaaacca ttgctaaatc gtcttcgaaa gaatcggaag ggactggctt agcgtaagaa
    22201 cttaagtcgt catggccttt ttcccattcg acgaaaccac cgaaaatact tcggcaagct
    22261 gaaccagacc cgcgtcgagc aaatcttgaa agagagagat catctaatcc aagcttaaga
    22321 gcagtattgc aagcgcctgc taaagcagct agcccgctgg cagaggaggc taatccagct
    22381 gcggtaggaa cgaaattctg actgattact gaagctttta gtgagaggcc ggcttcttta
    22441 cgaaaaaggt caagaaactg actgactttt tttgttgctt tttcgtcttg caaggtgcca
    22501 tctagataaa attcatctac catatagcta tcagaaaaga tgacttctgt ctctgtataa
    22561 aatgcatcca atgttaacga aaggctgttg ttcattggaa ggatgagttc ttcatttttc
    22621 ttaccccaat atttgattag agcaatgttg gtatatgcgc gtgctttgcc tttaaacata
    22681 ggtatgtact cctaatcctt gtatccaagt ttcagcagca cccgcatctt caagtgcttg
    22741 actgatttct tgggcttttg tttttgtttg tgctaatgca atcatacagc caccgcgtcc
    22801 accgccagtt aatttggcac ccaatgcccc ggtatctttt gctgtttgga tcaatagatt
    22861 aagaaaatca ttgctgattg tcagcttttc cagaagtgat tgtgattcat ccattgtctg
    22921 tgccaacgtt tctggtgaat tttcaatgat tgcttgtttt gcttgcttcg tcaagtatcc
    22981 taatttttgg atcatttgtc cagtttcatg aggctgggtt tcaaagaggt gagcgacatc
    23041 tttgacggct tctcttgttt gtcctttgat tcctgtatca gcgacaatca aaaaagcatc
    23101 gatgttcaaa gaaaagtagt cgaaaggatg cccttttgta aaataaatcg gctgcaagct
    23161 gctagtagcg gctgcatcga ttccactagg attaccatgg gcgatttttt ccgaaagctg
    23221 gacattttct aatagtattt cacgagacaa aggaaatgct aagtaatcat aaaaagcacg
    23281 agtgactgct gtggcgactg cagcgcttga tcccattcca cgttcagcag gaatcgtgct
    23341 ttcaatggtc agttgcaagt ttttttggat gtcatgctgt ttttttaagt gaatgaataa
    23401 ttttttgata ttttttaatg catgaggagc ttcggctaaa tttcctgaat agtaggaaga
    23461 aaccagttga tcgtccattg catccagctc ttcaaggaat gcggtgactt ttgttgcata
    23521 gaaaggaaag gcaatcgctg gttctccata aacaaccgcg tgctcgccca tcaatatgat
    23581 ctttccgctt gattctcctt ggccatagtt tgccataatc taactccttc tcacgtaaaa
    23641 gtgattttta agcaaaaaat aaagcgccca aaacgagttg cgctgcttca cgaatttttt
    23701 tgtccactaa gggtctcaaa aaacattcct cctttatttt actaaaacca tctcagaagt
    23761 aaaactaaga atacgttttc taaagaaaaa acactttttt cttaagaatt ttttgacttt
    23821 ttttcttctt tgactagaaa aaaatacaat tgacactact agatattggg ggtatattta
    23881 aaaaggttgg agttaaacac aatatttagt gatgaagtga gctggaggta aatgagcgtg
    23941 atcgttttag caggtacaat tggtgccgga aaatcaagtt taacagaaat gatggctgaa
    24001 cactttgata gtcaggcatt ttatgaatcg attgatgata atgaagtctt gcccttattt
    24061 tatgcaaacc ctgaacagta tgcttttttg ctgcaaattt attttttgaa taaacgtttt
    24121 gcaagcatca aacaagcaat gaaagatgac aataatgtat tggaccgttc gatttatgaa
    24181 gattcactgc ttttccatct aaatgcagat ctaggaagag caacagaaac agaagttcgt
    24241 gtgtatgatg aactgctgga aaacatgatg gaagaacttc cctatgctgc ccataaaaaa
    24301 catccggatt tattagttca tattcgtgta tcttttgata cgatgttgga gcgtatcgaa
    24361 aaacgtggtc gttcgtatga acaattgtcc tttgatccaa gtctatacga ttattacaaa
    24421 gaattgaaca gacgttacga tcaatggtat gaagaataca aagaaagtcc aaaaatccaa
    24481 atcgatggag accgctataa ttttgtggaa gatcctcaag caaaagaaga agtcttgaaa
    24541 atgatcgaag aaaaattagc agagattcgc caaaccaaag tagccatttg atttacagtt
    24601 gctcaaataa agggtaaacc tttatttgag cgactttttt ctttatactg gaaacaaatg
    24661 tcaggaggat agagtatgcg ggaaaaacac tattttaatc aagaccgtaa tttttggatc
    24721 aatgtacagc aaaacgatgt gctagctgtt aaaaacttaa aagaagaata tggcattagt
    24781 gacgagatgc tgacttattc tctagataaa aatgaacggg cgcgcgtaga gtacgattct
    24841 tttgatgaag cgttactgct tgtatcaaat gttccgcacc agcaaaaagt cgataatcat
    24901 tatgaaacta gtcctatcgc ttttatttta aaagaagacg gtttgtttac atttaccacg
    24961 ccgaataccg agtacgtgat tcgactaatt cgatcgcttt tggaacgaat gccagacatg
    25021 agtgtataca gtttattgtt tcgtacattg tttttgattt ctgattcctt ttttccttta
    25081 atcgaggaag tgaatagtga acgtcagcgg cttaatctga aattaagaga aaaaacaacg
    25141 aacaagaatc tgctgcaact ttcagattta gagatcggtt tggtttactt agttacagga
    25201 acaaaacaaa atgtagtact tttagaacaa atcaaggcat tggctattta tcgcaagttg
    25261 tcagaaaagg aaaaagaaga gttggacgat gcgctgatcg aagcacgaca agcagttgaa
    25321 atgacgaact tagcttcgca gattttagac cagctctctg gcacttataa taatttgctg
    25381 aataacaact tgaatgacac gatgaaattt ttgacggtct ggtcgctgat cttgaccgtt
    25441 ccaactattg tgacaggatt cttcggaatg aatctccagt taccctttac ccattccgtt
    25501 ttcggatggg gaatcgcctt gatcatcagt ttggtcttat ctatttggat gcttattgca
    25561 ctgtggcggc ggattcgttg atctagtctt tgatctgcaa accaagggct tgggcgatca
    25621 caagtgcttg ctcttggttg acgattagtc ccgccaaaga atcttgagat aaagcgattg
    25681 ctgtgaagaa attagtcgta aagtctaatc ccttcagaga agttcgaaac cagttcgaac
    25741 cggtcaactg attgtcagac aaaaaaaggt ttttccaagt gacctcataa aattcgctgt
    25801 cttctaattg acaatgatcg aaggagacag ctttcaagtt tgtactacta aaggaagcca
    25861 tcgaagcaat acaatcagtg aaagtacagt ctcgaagata actttcggca aaattcgtac
    25921 ctgttaattt acattgatga aacacgactt ggtggaaact gccaccaatc cactccagat
    25981 tggaaaaatc acatttttca aaaatgacat tactgcattc aaaatgacgt aaaactgttt
    26041 tttgcatcgt aagtttttgg atatgactct ctcttaatac catatttttg gcatcgagat
    26101 agctttggtc acccccgttg atcagaagac cagagaagat ctcctcatct tctaaataaa
    26161 actctccctt tgtaagaaga ggaagagaag gtggaatagg ttcgattttt ttcatgtggg
    26221 ttcacctcgt ttttctttag tatagcaaag aagcgggaat cactgctaga aagtctgcta
    26281 atctaatcat tttcagaaaa ggaagacaaa ctgtatgaaa aacattgaca aaaagattag
    26341 tttttgctat actatgatgg ttgagaaata aaagcagaag cacccgcttc tcgccttagt
    26401 tgataatgtc actgggctag atagttttca ctggttctat taggtgaagt acgcgcgggt
    26461 tctgtaagag aactcgtttt tttattgccg tttattcggt aagcttttct ttctctcata
    26521 atatttggag gtgaatgacc atagcaaagg atatgatggt taacgacggc attcgtgcac
    26581 gagagttacg attgatcggt tcagatggtg agcaattagg agttaagaca aaagcagaag
    26641 cattgaatat tgcagaacaa gcaaacttgg atcttgtgtt agttgcccct ggtgcgaaac
    26701 caccagttgc gcgaatcatg gactacggta aataccgttt cgaacaacaa aagaaagacc
    26761 gcgaagcgcg taaaaaacaa aaagtgatca atgtaaaaga agttcgctta agtcctacga
    26821 tcgacttgaa tgacttcaat acaaaacttc gtaatgcacg taagttcttg gaaaagggcg
    26881 ataaagtgaa agcttctatc cgtttcaaag gccgtgccat tacccataaa gaaattggtc
    26941 aaaaagtctt gaatcgctta gctgaagaaa ctgcggatat tgcaacagtg gaacaaaaag
    27001 cgaaaatgga cggacgcagc atgttcttga cgctggcacc gaaaaacgac agtaagtaat
    27061 ttgaatgact aactggctgt tggacagccg atagtcggat aacgaaagat tttgaggagg
    27121 aaatatagtc atgccaaaac aaaaaacaca ccgcggatca gcaaaacgtt tcaaacgtac
    27181 tggtaaaggc ggattgaaac gcttccgcgc gtttacaagt caccgtttcc acggcaaaac
    27241 aaaaaaacaa cgccgtcaat tgcgtaaagc aagcatggtt tctagtggcg acttcaaacg
    27301 tattcgccaa caactagcaa aaatgaaata agaagtacaa ataacagaat ctgaagattt
    27361 atttttatta ggaggaatta tacatggcac gtgttaaagg tggaacagta actcgtaaac
    27421 gtcgtaaaag agtgctaaaa ttagcaaaag gttactacgg ttcaaaacac actttattta
    27481 aaacagcaaa agaacaagta atgaattcat acaactacgc atatcgcgat cgtcgtcaaa
    27541 agaaacgcga cttccgtaaa ttgtggattg ctcgtatcaa cgcagcagct cgtatgaatg
    27601 gcttgagcta ctcaaaatta atgcacggtt tgaaagttgc tgaaatcgac atcaaccgta
    27661 aaatgttagc tgacttggct gttaacgatg cagcagcatt tactgcattg gctgaccaag
    27721 caaaagaagc tttggctaaa taagctaaac aattataaag aggaaagaag ataacgaaaa
    27781 gttatgttct ttcctctttt tttatttaca atcctccatc aaatgaaaca ggacaataac
    27841 gtataagtct aaaatagcat aattaaggtt ttttaattga aaactgaaca tttatggtca
    27901 ccctttaatg aaagcgctaa ctacataagt tagaatgagt ttgactgata tattagtttg
    27961 cttatcagaa aaacaaaatg gacggaggaa taacatgagt gatattatta ttggtatttt
    28021 gttagttatc tcattctttt tcatggtctg gtactgtgta aaaggctaca acttgatggt
    28081 cggctttgcc atcatggcta ctgtatggat gggattagcg ctagtcggaa acacgttttc
    28141 accaaacccg gcgatggaag ggcaaggagt aattgatgtg ttgacgcata tctatacgac
    28201 ggggccagct gagtacgcca aatcgatctt ggtaaatgtc ttctttggtg cctttttcgg
    28261 gcgtgttcta gtagatagcg gaatcgcagc taccctgatt cgaaaggtag ttgaacttgg
    28321 aggggacaag ccaagaatca cgatgagctt actatgtatt gtaaccgctg ttatattcat
    28381 gtcgatgaca ggaatcggac cagtgatttc tattgcagtt attgtgttgc caatcttgat
    28441 gtcattaggt atttcagtac cggttgcact gttctcattt atgggctcga ttatggctgg
    28501 aatctttgct aatatcgtca atttcaaaca atatcaaacg atttatgctg gtttcaatcc
    28561 agcggctgaa agctatactt ataatgacta tttccaaatt ggtatgattg ggatgattgt
    28621 aagcttagtt gtggtactaa cggtcgctaa tatatcaatg aacaaaaaga aacgctatgc
    28681 aatggcagca aacgtaccag ctgaaggtgg cgacgcacca atgatctcat ggttagccgt
    28741 attattacca gttttagggg tcgttctttt agatcttcca atcatcttag gttttattct
    28801 agcaggtatc tgggcattgc tgtttactgg aaaattgcgc ggaggttata aagaaatctg
    28861 tcgtcagttt gcgaaattat ttacagatgg cgcagtagat gttgcgccaa tggttggttt
    28921 cttgatgacc ttagcgatgt tcaataattc agctgcgtat gcttctcctt atttttcagc
    28981 tatttttgga gattggatac cgcaatcacc attagtcttg gcaatcgtat ttgctatatt
    29041 gacaccacta ggatttttcc gcggtccaat gaatctagtc ggttcaggct cagctatttt
    29101 agctgtcgta ttagctgtta atccaacgat gtcgccagcc tttttgttcc cgttatttgc
    29161 aatcacaacg attgctcctc agcatttaga tattacacaa tcttgggttg cttggggatt
    29221 aggttataca aaagtaactt ctcgagaata tatgaagaaa tccatcccaa ctggttggat
    29281 catcggagcg atcttatgcc tgatcacgtt cttattgtac gggaacgcct agtaaacaaa
    29341 acgtcgataa aaacgattag atgaatggag gaaaagaaaa tgaaacgttt tgtgcgaatg
    29401 ggaattgatg ttggcggtac acatacgaaa gcagtcgcaa tcgataatgc gacgcacgaa
    29461 atcatcggaa aatcttcagt gaaaacaacc cacgatgatg tccgtggagt agccgcaggt
    29521 gtcgttcagt cttttcaaaa ttgtttgctg gaaaataata tcagtccaga agatgttgtg
    29581 tttgttgcac attctaccac acaagcgact aatgcactga ttgaaggaga cgtagcaaaa
    29641 gtcggcgtaa tcgggatggc aaaaggtgga ttagaaggtt ttttagcaaa acgacaaact
    29701 agattgaacg atatcgattt agggaataag aaaaaaatag agattgtcaa tgcattctta
    29761 cctgtcaaac acttaaatgt agaccgtgtt tcagaaacga tttcttcttt agaaagagaa
    29821 agagcggagg tcttggtttc ttctatggca tttggagtag ataatgggga accagaacga
    29881 gttgtttatg aagcagcatc ggtaaaatcg atccctacaa cgatggcttc tgacatcaca
    29941 aaattatatg gattgacaag aagaactcgg acagcagcaa tcaatgcatc tattttacca
    30001 aaaatgctag atacggcaac ctctacagaa gattccgtac gagaagcagg ggtcaatgtt
    30061 tctttgatga ttatgcgcgg agacggcgga gtaatggaaa tcaatgaaat gaaaaaacga
    30121 cctgttttaa ccatgctttc cggtccagca gcatcagtca tgggctcttt gatgtacttg
    30181 agagcgtcta atggtgttta ttttgaagta ggcggaacaa caaccaatat cggggtcatt
    30241 aaaaatggac gtccagcaat cgattattca attgtaggtg gccacccaac atatatctct
    30301 tcattagatg ttcgggttct aggtgtggca ggcggttcga tggtacgggc taatcagtca
    30361 ggaatcatcg atgtcggtcc acgttctgca catattgccg gtctagatta tgcagtattt
    30421 acggagacag aaaaaatcaa aggtcctaag gttgaatttt tctctcctaa agaaggagac
    30481 cctgctgatt acgtcaaggt agtgatggaa gacggagaag aagtgactat tacaaataca
    30541 tgtgcagcca atgtccttgg attagtgcaa gaagaacatt tttcttatgg aaatgtccca
    30601 tctgcacgaa aagccatcca agcattagct gattattgtc atacgacggt tgaagacatc
    30661 gcagaacaga tcatggaaaa atcctatgct aagattgaac cggtgatttt agaattggca
    30721 gacaagtatc acttggagaa agatcagatt tctcttgtag gtgtaggggg cggcgctgcc
    30781 tcattgatta cttactttag taataaaatg ggtgtgaaat attcgattcc tgaaaacgcc
    30841 gaagtgattt cttctattgg tgtagcactt gcaatggtcc gagatgttgt agaacgtatc
    30901 atcccttcgc caagtaaaga agatatacga tcgttgaaaa atgaagcgat gaataaagcg
    30961 attgaatcag gcgctacacc agaatcgatc gaagtccacg tagaaatcga tccacagact
    31021 tcaaaagtaa cggctatcgc tactggttct acggaagtga aagcaaccga tctaaccaag
    31081 gagatcacaa cagaagaagc actagagctt gctgcagagg atatgcgatt aaataaaaat
    31141 gaagtttgct tattggaaaa tacacctttc ttttatgtat gcggcgaaca gaatcgttca
    31201 aaaaatgctg gaagtcttcg gatcatcgat caaaaaggat ttatcaaggt ccaacgtgga
    31261 catgcttcat gtatgaaaac aactgcagcc aattatatga cagctgtcga acaactttgg
    31321 gaagatatgg cagtttatca aactgaattg attgcccgac cagaatttta tctctgctta
    31381 ggagcaagag tctctgattt tacagcgacg gatctggaac aactacagct tttgatggat
    31441 ctggaagtat ctactatgga accggaggaa gaagtgatcg tcgttgccgg aaatatcaaa
    31501 caaacataag ttagaagagt tactaaacga attacaacag atccccgaag aaatttgggg
    31561 attctaccag tttcggcgag atttattttg gaaaaagatt cctttgtcca agcagaaaat
    31621 tcttatacaa caatcaatcg attgtggtat cgaaacggca tgtagcatca aaaaaaagta
    31681 cccttttgcc gatgtaggag aaatttgtga acaaatggct gttccaatcg tttcctgtga
    31741 atcagagcaa ataaacgaac gaatcacttt tgcaacttat gcagaagatg aagggattcg
    31801 attgatgacg gagccgttag aaaaattaaa atgttcagga ctagcttcga tctctaaaga
    31861 aacagcgcaa gctttgatta ttggacatga actatttcac cacatcgaag cttctgttaa
    31921 gggaatttat acgcaaagcg aaaaaattgt gctgtggcgg ttgccttttt atacacatca
    31981 atcaaacatt cgtgcattga gcgagatagc agcgatgagt ttttcaaaag agatgaatca
    32041 aagcgatttt tcgccttatg tattagaggc agtcttactt tggccatata atgaaattcg
    32101 tagccaaggg atcttagaag aaataaaaaa aatagaaaaa agatgtacgg agtatgattt
    32161 agattacaaa taaagagact gtgacaaaag tcgtgtcaca gtcctttttt tccgaataaa
    32221 cgatgttctc ctgtcaactc ctcggtcaca agtcaaaata ttcaacaaac atgagaagct
    32281 gttttttgaa attgttcctt gtgactcgga gatgaacggc aggctcacga cctcttggaa
    32341 aacggtgttc aaaagcttgc atctgcggta ataagtccag ccaagttaaa aacataagaa
    32401 gctattttta cttggctgtt cttattactt gatgcagggc agctttttca caacctctca
    32461 ataaacggtg tgcaatcagc tgaccctcgg tattagctca aaaaaaacgg caaaatatga
    32521 ggaactattt tgccgtttct ttctctaata ctcaggtcaa aacgctgatt ccaaaacctc
    32581 ttatttgatg ggtctgtcct gaatatcttc aaaataatcg gggtaattat gaatcacgta
    32641 ttcttgctcc tcgataatca tatgtaaatg catcgtgcaa agaaatccga cttcatcaaa
    32701 gttatgatca atcattgatt gaagcattgt ctgatgttca tctaatactc gaccccagtc
    32761 taattctgaa atcgtcaaac gtaaccatct gaaacgcgta ctataacttt ctagccaatc
    32821 ccaaatttct cgttttccag caatttcata acaagttcgg tgaaaatcag tatccaaatc
    32881 aaaaaaagct tttttatttt gagcgattac tgcctgatcg gtttgttcta gtattttttc
    32941 tagtttagct tgatcttgct tggtcatttt cgcactgcat tcttgcagga tcggtttttc
    33001 caacttttcc cgcgtataac gggctaatga agcagatcgg atatcgattt ttgaaacata
    33061 agtgccggac tgtggaatgg tttttacaag ctcttgattg cgcagttgga tcagtgcttc
    33121 tcgaatcggt gtacgcccga tagctagttg ttcttctaac actttatcgg agattttact
    33181 tccaggtgct aactcggaat aaatgatttg tttacgtata gattcataag cttgctgttg
    33241 caaattattg ataaccattt aaccttcctt tctatgcaga tataatctat tataacataa
    33301 atcgcttaca aatcgtgtgg gcataaaaac acagtctgca aaaaaagccc tgataggcat
    33361 gcctattaga gcctcgaaaa gaaaaatatt gaatcaatct ggtcgtacct cttggatcgg
    33421 tttgacacga ccgccgattc ttttagcaaa agcttcggat tcttttttgt ctttgaaaat
    33481 gagcagattg tctaagtttt tttcccgagt gcgacagcca gttttatcga tgtaaccgtg
    33541 cattacttta acgacataca tggggatcac ctgctttttt cttttcatta tacgaactac
    33601 tcgacacaat acaagaaaaa tgcttagacg gtctttatta agaatttaca tcatccaaag
    33661 aagcgtacgc ttggaataac tgatctaact ccggattgac tttgctcagc ttcactaatc
    33721 gttgcgaatc agaaagtaaa aaacagtgtt tactactgcc agtagttttt aattttttgt
    33781 cttcagttcc cacaatttcg tagctaaaat ccaaccgtgt ccccgtatac ttttggatag
    33841 ttggatggat gctcactttt tctccgtaat ggatcatttc tttatagctg caatttactt
    33901 ccagaacggg gatgatgatc ccagattctt cgattttttt atagggaaac cctagatgta
    33961 ccaataaggc tacccgagcc tcttcaaacc agcggatata attgctgtga tgaataatcc
    34021 ccatttgatc tgtttcataa taaaaaggtt ctcgcacata acctgtaaat ttctccatga
    34081 ctcatgcctc gttttccatt gattttttac aaaaataatt catccatttc ggaagtgctt
    34141 cgctgatttg tgtcggcaaa acaatatatt gttcttttgc cagctggtct ccaatgaaac
    34201 tatgaagata aatagctgct gcaatcgttt ggtcattttg aggaaactgg gctaaaaatc
    34261 ctgtgatcat cccagctaaa gtgtcaccca tgccgcccgt tgccatagca gcagaaccta
    34321 aaggattggt gtagaagtgc tggtgaccat aaatttttgt ctcatgactt ttcaaaacga
    34381 gcacagcacc gatttcctgt tgcttctttt tgttttcttt atcccgttgt tcttgtatcg
    34441 gtagagaact caaccgttgc cactccattt gatgaggtgt aagtacagtt tgttcgggat
    34501 agggtagcaa aagatgatta gcagcaagta aacttaaagc agagccatca atcaccagcc
    34561 attgttcttt tgattggttt tctagtactt gtttgaaacg ttccaaactc ttagaagaag
    34621 tacccaatcc gggaccaatc aagagtacat ctgcttgctt tattacggaa agaaactggt
    34681 cagtatccgt ccaaactaca atcattgctt ctggcagatg actgtgaaga gcactccgat
    34741 ttttttcatc agttactaca gtcgtgagac ccgctccgct gtgtacggca gctaaagcac
    34801 tcataatgat tgctccgcca tattgttcat tgccgccaat caaaacgata cgtccaaatg
    34861 ttcctttatg tgaaattttt ggacgaggtg tgatgactct tttcactagt tgttcgttta
    34921 tcagcatcta aaaacctcct tttactcatt atagcgtata ttttttttag agaaaaacag
    34981 ataagaaaag gaattgtcag attttttgaa ctattttttt ctgtaatttg tggtatcatg
    35041 aacacgaaga cttaaataag tggaggatta actttatgac aattgattgg caaaaagaag
    35101 tagaagcacg taaagaagac ttgcttgaag acttgaaaaa tcttttacgt gtcaacagcg
    35161 agcgcgatga ctcaaaagta acaccagatg caccatttgg acctggacct cgcgacgcat
    35221 tgaaacatat gttagcttat ggcgaacgtg atggatttaa agtaaaaaat gtcgataact
    35281 atgcagggca tattgatctt ggcgaaggag acgaaacgct agggatattt ggacacatgg
    35341 atgtcgttcc tgcaggagat ggctgggaca cagatcctta tgaaccagta atcaaggatg
    35401 gaaagatctt tgcacgcgga tcaagcgatg acaaaggtcc aagtatggca gcttactatg
    35461 cgatgaagat cattaaagaa cttgacttga aactatcaaa aaaagttcgc ttcgtagtcg
    35521 gcagcgatga agaaagcggc tggggcgata tggcttatta tttcgaacat gaagaagaac
    35581 cagattttgg tttctcacca gatgctgaat tcccaatcat caatggtgaa aaaggaaatg
    35641 tttctttagc tttacgcttc aaaggcgaca atgcaggaga ttatgtcttg aaatcttttg
    35701 tttccggact tcgcgaaaat atggtgccag gaacagcaac tgcagcactt caagtaccaa
    35761 gtgcagatgc agctatcgcg atggaagaag cattttacca attcatcgaa gcaaatccag
    35821 taagcggaac gattgaagcg gacaatacgt atgtaaaaat cgaattagtt ggtaaaggcg
    35881 cacatggtgc aagcccacaa tctggtatca atgcaggttc tttcttggca ttgttcttag
    35941 ataactatga attcctagga tctgctaaac aatttatcca tgtagcagct gcttatgtcc
    36001 atgaagattt ttatggagaa aaactaggag tcgcttatga agatgaaaaa atgggcaaat
    36061 tgacaatgaa tgctggacta tttgcttttg aagaaaatgg tacagaggaa gcgaacttca
    36121 tcaatatgaa tttccgtttt ccaaaaggtg taacagtaga cggattgcaa agtgacatcg
    36181 aacaaacagt ggggcaagaa ggcgcaacag ttactcgagg tgctcgcgta atggaaccac
    36241 attacgtacc aatggatgat ccattggtgg ctacattatt gcaagtctat gaagaccaca
    36301 ctggagaaaa aggctacgaa caaatcatcg gtggcggaac ttacggacgt ttgttgaaac
    36361 gtggcgttgc ttatggagcg atgttcccag gatatactga tacaatgcat caagcaaacg
    36421 aatttatgag tcttgatgac ttgttcagag caacagcaat ctatgcagat gccatctatc
    36481 gtttggcgaa ataattaatt aaaaagagga tcaagaattg caaattattt ttgcagtctt
    36541 gatccttttt ttactttcta ctgtggtatt catgataaac atgatataga ttcaattcat
    36601 attctttctt agcgttcgtg acgactgtat ccaaaatcaa ccctgttact aatgaaagaa
    36661 gtgctaaagt catcaatcca gtacttaaaa tcgcagaagg gatgcgtgta ataaagcttg
    36721 tcagcatata ttcgcgaatc acaggaatcc ccgtgaccaa tccaaataaa aagaagaaga
    36781 tggtccaaat cccaaagaaa agcaaaggct tataatcttt aaacatttta ataatcatca
    36841 tgatcacttt gaatccgtca gagaatgtat tcaattttga ttcgctgcct tcaggacgat
    36901 cacgataatc gattgggatt tcaacgaact tgaatttttt atctaaagca tggatcgtca
    36961 attccgtttc gatttgaaaa ccagaactca taataggaaa acttttgaca aagatacgat
    37021 tgaatcctcg atagccggtc atgacatcaa gaatttttgt tttatacatt ctagtaattg
    37081 tatttctaac aaggttattt ccaaaatcgt ggaaaggtct cttattctca tcaaagtagg
    37141 ttccatttga taggcgatca ccaatgacca tatctgcttc accagaacgt aatttctcga
    37201 gcaaaccatg tacagcttct gcaggataag tatcatcgcc atctaccatt aaataatagt
    37261 cggcatcgat atcaaagaac atttggcgga tcacatttcc ttttccttga cgaggttctt
    37321 tttttacgat tgctccacct tgaacggcca attcgtatgt tcgatcggtc gaattattat
    37381 catagacata aatatctgct tctggtaatt ctcgtttaaa gtctgcaatg actgttgaaa
    37441 ttgttgcttc ctcattgtaa caaggaatca gaacagcaat tttttcttgt tgttccattt
    37501 ttcactctcc ttaactatag aaaacaaaag ctaaattaaa aatacctcat aatcaaagga
    37561 aatgctagtt tttcccgcta aatatcattt atttaaaaag aaaatactgg aatatatgtt
    37621 tggaaagctc tatctaaaaa accacccact acaaaagaag gacatgatac ctctcttttg
    37681 aatagtgggt gcgtattcgt tctctcactt ttttgacgta ttaatctgtt gcaccttttt
    37741 ttcttgaaat ttctaatacg gttcctgtat gagcatctgc taaaaattca taataaacag
    37801 tttcgccatc ttctattcga gaaagacctc cccgataagc actcgaccga acggcgaatt
    37861 tcctaatagg ttccttttca aaagagatcc aagaaccttc gattgcacct tcttttagaa
    37921 aagatgactt gatattttca agtacttggt ctgctgacaa tacttttttg cgatgatacc
    37981 aaatcgttga agctaccccg ctcaataatc ctagaccgat tccaactgct aacccgcctt
    38041 taaaatagct tagttcgctt tcttcataca tgtggacata cctccttctt ctcacttgtt
    38101 actccttgtg cttattttac cacactctca tattttcttt gaaataaata accgctacca
    38161 gaaaaaataa gctataataa atacgatcgg tctgcagaaa aaatcatatt ttatccgcag
    38221 atcaatataa gtctgagagg agtttagtct gaaatttaga cgaatagcat atggaagaaa
    38281 aaacatttca acgaatcaaa gagttaacag aattacaagg aacaagcggt tttgaacaag
    38341 atgttcgcgc ttatatggaa agtcatatgg aaccgctggt agatgagtta cagttagatg
    38401 gtttgggcgg catttttggt ctgcgccatc atgaagaagc ggatgcaccc cgtgtaatgg
    38461 tcgcagcaca tatggatgaa gtcggattca tgctgacaca aatccaagaa aacgggcttt
    38521 ttaaagtcgt acctctaggc ggctggaatc cttacgttgt ttctgctcag cgttttacac
    38581 ttaaaacctc tactggtaag aattatccat gtatttcttc ttccgttccc ccacatttat
    38641 tgcgaggaac aagcggacaa aaatcagtag aagtaaccga tattttgttc gatgctgggt
    38701 ttgaatcaag agaagaggca atgagttacg gtgtccttcc aggagacaca atcgttcctt
    38761 acgctgaaac gatcaaaaca gccaacggaa agaatattat cagtaaatca tgggacaacc
    38821 gctatggctg tacaatggta ttagaagctt tagaagcgtt gcaaaatgaa acattaggtc
    38881 atacattgat tgcaggcgcg aatgtccagg aagaagtagg cttgcgtggt tctaaagctt
    38941 ctgtcaataa attcaagcca gatctgttct ttgcagtaga ctgctctgca gcagatgata
    39001 cagtgacgaa aaatggcaca ttcggacatt taggagaagg tacattgatg cgtatccaag
    39061 atccaggttt gatcatgctt cctcgtttgc gcgagtactt gctggatatc gctgaaacaa
    39121 acaatatccc ttatcaatac tttgtttcaa aaggcggtac agatgcaggt gctgctcata
    39181 cacaaaacga aggaatccca agtacagtga ttggcgtagt gggacgttat atccatacgc
    39241 atcaaacgat gttcagtatt cgcgattttg aagctgcacg agaaatgttg attcaaacac
    39301 tgaaaggttt ggataaatca acagtaaata cgatcgtata cggaaaataa tgcgatagtg
    39361 aaactataaa atctggcaaa agattgcttt ttgccagatt ttttttataa aaagaggatc
    39421 aatgtttgtt agttcgaaag aaaggtgtat aatttaattg aaaaatacat aaacagaaag
    39481 tagaggacaa agaatgatta tcccaaatag tttagaagaa ttagcaggct acgtagaaac
    39541 tggaaagagt gtatttttct ttactgccgg ctggtgcgga gactgccgtt ttatcaaacc
    39601 gcaaatgcct gaaattgaaa atgacttttc tgattggcaa tttattgaag tagaccgtga
    39661 caagtacata gatgtagccg ctgaatggaa catttttggt atcccaagtt ttgtcgtgat
    39721 ccaagacgga aaagaattag gtcgtctagt gaataaagat cgtaaaacaa aacaggaaat
    39781 cgaatcgttc ctagaaagtg taacaaaagg ctgacatctg ttaggagggg tcaagatgaa
    39841 tcaagaatat aagaatattt tagtaggtat cgacggtagt gaacaagcac ttgctgcttt
    39901 caaaaaagcg gtagaagtgg ctcgccgtaa taatgggaaa gtatatgtag caaacgtaat
    39961 cgatcagcaa ttttataact ttatgggcta tgcacctatt gatcaaaatc tagtcgatca
    40021 gcaaactgaa acagccaagg aaatgatcga agaatgtaaa gaatatggga aatctgttga
    40081 ttacacaaat atagaaggaa ttattgcata tggttctgtt cgtgaatcca tggcagtcaa
    40141 attgccaaaa aaatatgaga tcgatctaat catggttggg cagtcaggtc taaatgcagt
    40201 cgaacgcttt atgacaggaa gtgtagccag ctacgtgata cgtcattcac cttgtgatgt
    40261 cttgatcgtt tctgacgaag aagaacagcc aagtaattaa gcaagtggaa cttgtacgac
    40321 ctttttaatc aattaactaa aacagtaaag ggactgtgac aaaagtcttg tcacagtccc
    40381 ttgttacgaa taaatggtgt tccagaagag gcttcttcgg aaataaagca tgaaaaaaat
    40441 ttcatgcagt gaatgcaatt ttttgttaag ataaatctca gcaaaggata atggaggaaa
    40501 gaacatgatt tttgcttata acaaagcaca cgtcggagac accttgatgg ttattatagc
    40561 cgacgataaa ggaacagaaa acactgttga acgcaaatgg aatgttgctc ggatcaaaga
    40621 tgaacaaggt caagttgttg cttggaactt tttccatatc tccgatcatc taacaatcga
    40681 agggaacggc caagtaagct taacagatga ggaattagcg aaattgaatc gattgatcaa
    40741 agaagcagga ttcgaagaac agcttgaagc tgatcatgaa ccgaagatcg ttgtcgggta
    40801 cgtaaaaaca tgcgtcccac atcctgattc tgaccattta tcgattactg agacagaagt
    40861 agacaatggt acagtattgc aaatcgtttg cggtgcaccc aatatcgaag ctggacaaaa
    40921 agttgtcgtt gcaaaaccag gagctatgat gccagatgga atgatgatct ggccaggaac
    40981 tttgcgaggg gtagatagct atggaatgat ctgttccgct cgtgaattgc atttgccaaa
    41041 tgctccagca aaaaaaggaa tcttagtatt gcctgaggat gccgtagtcg gagaagcatt
    41101 tccaaaataa agaggttgtg acaagtcttt gtcacaacct cttttttatt aattttcact
    41161 attttgattt tgttttaatg ccgattgatc gatcgtcaat tcaacagtag ttgttttttc
    41221 tttcgagcct tcatagtaag tgattttgac agaatctccc acttttttct tatacaaagc
    41281 agattgaagt tctacaccag aagaaacttc tttgtcatcg atcttagtaa tgacgtcgta
    41341 ttgtttcaat ccagcttttt cagccggtgt agctgtttgg acatttgtca cgatgacacc
    41401 gttagttaca gatgaaggaa tcttcaagat ttgttcttgt tgctgagtag aaacggcaga
    41461 taagtcgacc attgtgatac ctagagctgg acgtgtcact tttccgtctt tttccagttg
    41521 attgatgata ttcactacat cgttacttgg aatagcaaat cccattcctt ctacgcttac
    41581 attagaagta gattcagaag tacttgcgat cttacttgag ttgataccga taacttgtcc
    41641 ttcgatatta actaaaggac caccagaatt tccagggttg attgctgcat ctgtttggat
    41701 cgcattgata ttgactgttt cgttcgattc attcgtactt gtcacttgtc ggttcaagga
    41761 agaaatgatt cctgaagtga cagaatttgc atattcagat cctagtgggg aaccaatcgc
    41821 aatcgcaggc tcaccaacct tcagagcact tgagtcacca aaagaggcaa ctgtttcgac
    41881 tttgtctgaa ttgatcttca acaccgctaa atctgaataa gcatctgttc caactaattc
    41941 agcttttacc ttcgtaccgt ctttcatcaa gacttcaaga ccttgctggc catctactac
    42001 gtgattattc gtgactacat aagcagtatt cccgtctttt ttgtagatca cgccgcttcc
    42061 ttcactataa gcttccaaat tactatcatc tgagctgcta ctttcttctt gctgcccgaa
    42121 tagtccgcca aaaccgcttg attggttttg actttgtaag ttgataacag aaacgacagc
    42181 gtcttgaact ttatctaccg ctttggtgat atcagaatct acattgactt tgacattttc
    42241 cacaactgtt tcaccggcag aattctgatt gccgcttgta gcagtagaac tattgttgcc
    42301 agtgcccatc gctgcataaa aaatgccagc tgtcagtagt cctccaacga tccctccaac
    42361 aagccccaga cctaacttac gccagaggct attattttta ctttttttca tctttggtgt
    42421 cacatttttt ctatccatgt gttcctcctc caattacatt catttatcta ctgttagtat
    42481 agtcttattt gttgacttta gtctactttg agactgtgaa ctttttatga aaaactttag
    42541 gaaaaatcat tgttttcaga tgaaaatatt tgattttctt tttccacaga tactgtgtaa
    42601 aactacgcgg ataggattgg aaaaataagg gataaaacat atccacagcc tgtgtataaa
    42661 gtggataaat tcttataaaa cgtatgtttg cctaatgaat gtgatgaaat aaggtaaaat
    42721 aacactgttt tctgtggata aacctgtgga tgttgtgcat aactgttggg gaaatctttt
    42781 taacaaaaat agccaaaata aacatataga cgaaaaaatt gattataata aacaaagtga
    42841 gcatgaagac caaaggagag aaaacatgaa tatcaaaatt atctcagtag gaaaattaaa
    42901 agaaaaatac ctgatccaag gtatcaatga gtatgtcaaa cggctgaatg cctatgcaaa
    42961 aatcgaattg atcgaagttc ctgacgaaaa agccccggag aatttaagcg aagcgcaaat
    43021 gagacaagta aaagaaaaag aaggggaacg gatcttagcg aaaatcaaag aacaagaata
    43081 cgtctatgcc ttagcaattg aagggaaaaa tcctacaagt gaatcttttg ctaaacaaat
    43141 cgaccaatta ggtatccaag gaaaaagcca ccttgttttt gttatcggcg gctctttagg
    43201 actcagcgaa gccgtcatga aaagaagcaa tgctcagatc tcttttggaa aaatgacgta
    43261 tccacatcaa ttgatgcgtc tgatccttgt cgagcaaatc taccgtgcct tccggatcaa
    43321 tgcgggggct ccataccaca aataatgata ttttttaaga gaaaaaatta tccctatttt
    43381 aaataatagc aataggtagt ggtagggaga aatttataaa taactttttt atttgctttg
    43441 gtagccaagt aagttgcaaa agaaaaatag atactgttaa aaagataatg tagacataga
    43501 aacaaatacg taaaatttta gttagaataa tggcaaggag aaaaaacatg atagatgaaa
    43561 aaaagtgttt aaaaaatcta ttagaggata tggatgatat tgaagaaaac tatctaaatg
    43621 gactgggaaa acaaatatat ttcagatact taagctatct aaaaatgaaa taagacatag
    43681 taatttttta gcttttttgt ttgctccaaa tgaaacccac aatttatctg atgagttttt
    43741 taaaatgttt ttgaaaaggt atatagattc taatgatgat accaaagctg ctatcaatta
    43801 ttttgatgct ttacttaata gctatgaaga tttaattgtt tacagagaaa ataagaatat
    43861 tgatatttta ttggtatcgg aaaaaaataa aatagtggtg tgcatcgaaa ataaaattct
    43921 gtcttcggag tctggagggc aacttaataa ataccaaata tatatagaac gcaattattc
    43981 taactataaa aaaatttttg tttttttaac tccagatgga aatgaaccca caaatcctag
    44041 ttggggcata attacttaca agaatatcgt tgaagagagt gtaaaatatt ttgtgtaaat
    44101 agaaaaaagg aagtcccttc tgtagaatag agttaccaca acacattcac agaaaagagg
    44161 acttccatat gaacgatttt actacagaaa ttatggaaat actaatcaat aaaggtgatt
    44221 tggatgattt gtttcgtcgt cacctagaat tggctattaa cgctttatta caagctgaat
    44281 taacggtatt tcttgactat gaaaagtatg atagagctgg atttaattca ggtaattccc
    44341 gtaatgggaa ttattcgcgc tcttttaaaa cagagtacgg tgaattgaat ttagtaatac
    44401 ctagagatag gaatggagac ttttctcaac aaactttacc tgcttataaa agaaccaatg
    44461 actctttaga aaccactatt attcagctag ttcaaaaagg aattactatg tccgaaatct
    44521 ctgatctaat cgaaaaaatg tatggtcatt attacaaacc acaaactatt tcaaatatga
    44581 gtaaaattgt gtctgaagat gtttcggcat ttaaagaaag aactttagaa gctaaatact
    44641 cagtcatttt tatggatgct actcatattc ccttaaaaag acaaaccgta tcaaaagaag
    44701 ctgtctatat agtaattggt atcaggttgg atggaactaa agaggttcta ggatttacta
    44761 tagctccaac tgaatctgct tatgtttgga aagagattct tcaagatcta aaagaccgtg
    44821 gtttagaaga ggttttgtta gtcgtaacag atggtttaag tggtattgac gatagtattc
    44881 atagtgttta tccaaatgct caatttcaac aatgttgtgt ccatatatct agaaatatcg
    44941 ctcataaggt tcgtgttagt gatcgacaag aaatctgtag cgatttca
//
LOCUS       DAZBTF010000014        44850 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000014 DAZBTF010000000
VERSION     DAZBTF010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.014"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..604
                     /locus_tag="ACHGNS_000868"
     CDS             212..604
                     /locus_tag="ACHGNS_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFN6936832.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="ACHGNS_000869"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="ACHGNS_000869"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFN6936833.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2698..2943
                     /locus_tag="ACHGNS_000870"
     CDS             2698..2943
                     /locus_tag="ACHGNS_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936834.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            3165..4775
                     /locus_tag="ACHGNS_000871"
     CDS             3165..4775
                     /locus_tag="ACHGNS_000871"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFN6936835.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4752..4964)
                     /locus_tag="ACHGNS_000872"
     CDS             complement(4752..4964)
                     /locus_tag="ACHGNS_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936836.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            5067..5936
                     /locus_tag="ACHGNS_000873"
     CDS             5067..5936
                     /locus_tag="ACHGNS_000873"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFN6936837.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="ACHGNS_000874"
     CDS             6101..7372
                     /locus_tag="ACHGNS_000874"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFN6936838.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="ACHGNS_000875"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="ACHGNS_000875"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFN6936839.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="ACHGNS_000876"
     CDS             8763..9026
                     /locus_tag="ACHGNS_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFN6936840.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="ACHGNS_000877"
     CDS             9471..9920
                     /locus_tag="ACHGNS_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN6936841.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="ACHGNS_000878"
     CDS             9977..11395
                     /locus_tag="ACHGNS_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFN6936842.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="ACHGNS_000879"
     CDS             11395..12255
                     /locus_tag="ACHGNS_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFN6936843.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="ACHGNS_000880"
     CDS             12429..13202
                     /locus_tag="ACHGNS_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFN6936844.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="ACHGNS_000881"
     CDS             13349..13714
                     /locus_tag="ACHGNS_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFN6936845.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="ACHGNS_000882"
     CDS             complement(13862..15070)
                     /locus_tag="ACHGNS_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN6936846.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="ACHGNS_000883"
     CDS             complement(15089..15367)
                     /locus_tag="ACHGNS_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936847.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="ACHGNS_000884"
     CDS             15560..16216
                     /locus_tag="ACHGNS_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFN6936848.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="ACHGNS_000885"
     CDS             complement(16658..17455)
                     /locus_tag="ACHGNS_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFN6936849.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="ACHGNS_000886"
     CDS             complement(17452..18636)
                     /locus_tag="ACHGNS_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFN6936850.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="ACHGNS_000887"
     CDS             complement(18661..19908)
                     /locus_tag="ACHGNS_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFN6936851.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="ACHGNS_000888"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="ACHGNS_000888"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFN6936852.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="ACHGNS_000889"
     CDS             21888..22202
                     /locus_tag="ACHGNS_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFN6936853.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="ACHGNS_000890"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="ACHGNS_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFN6936854.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /gene="tmk"
                     /locus_tag="ACHGNS_000891"
     CDS             22887..23531
                     /gene="tmk"
                     /locus_tag="ACHGNS_000891"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFN6936855.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="ACHGNS_000892"
     CDS             23546..23875
                     /locus_tag="ACHGNS_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFN6936856.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="ACHGNS_000893"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="ACHGNS_000893"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFN6936857.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="ACHGNS_000894"
     CDS             24863..25687
                     /locus_tag="ACHGNS_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFN6936858.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="ACHGNS_000895"
     CDS             25680..26027
                     /locus_tag="ACHGNS_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFN6936859.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="ACHGNS_000896"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="ACHGNS_000896"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFN6936860.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="ACHGNS_000897"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="ACHGNS_000897"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFN6936861.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACHGNS_000898"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACHGNS_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFN6936862.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACHGNS_000899"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACHGNS_000899"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFN6936863.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="ACHGNS_000900"
     CDS             31684..32451
                     /locus_tag="ACHGNS_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6936864.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="ACHGNS_000901"
     CDS             32441..34483
                     /locus_tag="ACHGNS_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6936865.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="ACHGNS_000902"
     CDS             34764..34913
                     /locus_tag="ACHGNS_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFN6936866.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="ACHGNS_000903"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="ACHGNS_000903"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFN6936867.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="ACHGNS_000904"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="ACHGNS_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFN6936868.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="ACHGNS_000905"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="ACHGNS_000905"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFN6936869.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="ACHGNS_000906"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="ACHGNS_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFN6936870.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="ACHGNS_000907"
     CDS             39330..40412
                     /locus_tag="ACHGNS_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFN6936871.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="ACHGNS_000908"
     CDS             40412..41215
                     /locus_tag="ACHGNS_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFN6936872.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="ACHGNS_000909"
     CDS             41285..42025
                     /locus_tag="ACHGNS_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFN6936873.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="ACHGNS_000910"
     CDS             42036..43040
                     /locus_tag="ACHGNS_000910"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFN6936874.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..44848)
                     /locus_tag="ACHGNS_000911"
     CDS             complement(43085..44848)
                     /locus_tag="ACHGNS_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936875.1"
                     /translation="MLFLTGIAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDFKFDS
                     KYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEES
                     NPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQV
                     LTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEP
                     FVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAEL
                     PELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGI
                     YQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQH
                     IQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLI
                     LARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKI
                     YLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQ
                     EKDTFIDRITVQLSRNYPNPV"
BASE COUNT        14415 a         7827 c         9341 g        13267 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtag ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcgaaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg tgcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataacaatg
//
LOCUS       DAZBTF010000015        42906 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000015 DAZBTF010000000
VERSION     DAZBTF010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.015"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            804..1190
                     /locus_tag="ACHGNS_000912"
     CDS             804..1190
                     /locus_tag="ACHGNS_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFN6936876.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            1284..1475
                     /locus_tag="ACHGNS_000913"
     CDS             1284..1475
                     /locus_tag="ACHGNS_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6936877.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            1989..2321
                     /locus_tag="ACHGNS_000914"
     CDS             1989..2321
                     /locus_tag="ACHGNS_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN6936878.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            2231..2425
                     /locus_tag="ACHGNS_000915"
     CDS             2231..2425
                     /locus_tag="ACHGNS_000915"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFN6936879.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            2464..2610
                     /locus_tag="ACHGNS_000916"
     CDS             2464..2610
                     /locus_tag="ACHGNS_000916"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936880.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            3048..4388
                     /locus_tag="ACHGNS_000917"
                     /pseudo
     CDS             3048..4388
                     /locus_tag="ACHGNS_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4629..6245)
                     /locus_tag="ACHGNS_000918"
     CDS             complement(4629..6245)
                     /locus_tag="ACHGNS_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFN6936881.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKKIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            6363..6581
                     /locus_tag="ACHGNS_000919"
     CDS             6363..6581
                     /locus_tag="ACHGNS_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6936882.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            6776..7237
                     /locus_tag="ACHGNS_000920"
     CDS             6776..7237
                     /locus_tag="ACHGNS_000920"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFN6936883.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(7430..7669)
                     /locus_tag="ACHGNS_000921"
     CDS             complement(7430..7669)
                     /locus_tag="ACHGNS_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6936884.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(7693..9216)
                     /locus_tag="ACHGNS_000922"
     CDS             complement(7693..9216)
                     /locus_tag="ACHGNS_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFN6936885.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(9234..9356)
                     /locus_tag="ACHGNS_000923"
     CDS             complement(9234..9356)
                     /locus_tag="ACHGNS_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFN6936886.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(9386..10369)
                     /locus_tag="ACHGNS_000924"
     CDS             complement(9386..10369)
                     /locus_tag="ACHGNS_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFN6936887.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(10393..10542)
                     /gene="rpmG"
                     /locus_tag="ACHGNS_000925"
     CDS             complement(10393..10542)
                     /gene="rpmG"
                     /locus_tag="ACHGNS_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFN6936888.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(10563..10940)
                     /locus_tag="ACHGNS_000926"
     CDS             complement(10563..10940)
                     /locus_tag="ACHGNS_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936889.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(10972..11151)
                     /gene="rpmF"
                     /locus_tag="ACHGNS_000927"
     CDS             complement(10972..11151)
                     /gene="rpmF"
                     /locus_tag="ACHGNS_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFN6936890.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(11166..11435)
                     /gene="rpsN"
                     /locus_tag="ACHGNS_000928"
     CDS             complement(11166..11435)
                     /gene="rpsN"
                     /locus_tag="ACHGNS_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFN6936891.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(11958..13724)
                     /locus_tag="ACHGNS_000929"
     CDS             complement(11958..13724)
                     /locus_tag="ACHGNS_000929"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6936892.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(13684..15438)
                     /locus_tag="ACHGNS_000930"
     CDS             complement(13684..15438)
                     /locus_tag="ACHGNS_000930"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6936893.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(15548..16117)
                     /locus_tag="ACHGNS_000931"
     CDS             complement(15548..16117)
                     /locus_tag="ACHGNS_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFN6936894.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(16135..17550)
                     /locus_tag="ACHGNS_000932"
     CDS             complement(16135..17550)
                     /locus_tag="ACHGNS_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6936895.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(17561..18229)
                     /locus_tag="ACHGNS_000933"
     CDS             complement(17561..18229)
                     /locus_tag="ACHGNS_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFN6936896.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(18359..18943)
                     /locus_tag="ACHGNS_000934"
     CDS             complement(18359..18943)
                     /locus_tag="ACHGNS_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN6936897.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(19274..19507)
                     /locus_tag="ACHGNS_000935"
     CDS             complement(19274..19507)
                     /locus_tag="ACHGNS_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFN6936898.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(19522..20472)
                     /locus_tag="ACHGNS_000936"
     CDS             complement(19522..20472)
                     /locus_tag="ACHGNS_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFN6936899.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(20469..21311)
                     /locus_tag="ACHGNS_000937"
     CDS             complement(20469..21311)
                     /locus_tag="ACHGNS_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFN6936900.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(21313..22032)
                     /locus_tag="ACHGNS_000938"
     CDS             complement(21313..22032)
                     /locus_tag="ACHGNS_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFN6936901.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            22580..22882
                     /locus_tag="ACHGNS_000939"
     CDS             22580..22882
                     /locus_tag="ACHGNS_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6936902.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNTFKIT"
     gene            22954..24180
                     /locus_tag="ACHGNS_000940"
     CDS             22954..24180
                     /locus_tag="ACHGNS_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN6936903.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(24268..24660)
                     /gene="rpsI"
                     /locus_tag="ACHGNS_000941"
     CDS             complement(24268..24660)
                     /gene="rpsI"
                     /locus_tag="ACHGNS_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFN6936904.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(24676..25104)
                     /gene="rplM"
                     /locus_tag="ACHGNS_000942"
     CDS             complement(24676..25104)
                     /gene="rplM"
                     /locus_tag="ACHGNS_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFN6936905.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(25638..26105)
                     /locus_tag="ACHGNS_000943"
     CDS             complement(25638..26105)
                     /locus_tag="ACHGNS_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFN6936906.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(26225..27763)
                     /locus_tag="ACHGNS_000944"
     CDS             complement(26225..27763)
                     /locus_tag="ACHGNS_000944"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFN6936907.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            27843..28529
                     /locus_tag="ACHGNS_000945"
     CDS             27843..28529
                     /locus_tag="ACHGNS_000945"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFN6936908.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(28730..29257)
                     /gene="msrA"
                     /locus_tag="ACHGNS_000946"
     CDS             complement(28730..29257)
                     /gene="msrA"
                     /locus_tag="ACHGNS_000946"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFN6936909.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(29341..30018)
                     /gene="pgmB"
                     /locus_tag="ACHGNS_000947"
     CDS             complement(29341..30018)
                     /gene="pgmB"
                     /locus_tag="ACHGNS_000947"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFN6936910.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(30015..31145)
                     /locus_tag="ACHGNS_000948"
     CDS             complement(30015..31145)
                     /locus_tag="ACHGNS_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFN6936911.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(31145..33328)
                     /locus_tag="ACHGNS_000949"
     CDS             complement(31145..33328)
                     /locus_tag="ACHGNS_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFN6936912.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(33325..34350)
                     /locus_tag="ACHGNS_000950"
     CDS             complement(33325..34350)
                     /locus_tag="ACHGNS_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN6936913.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(34395..35222)
                     /locus_tag="ACHGNS_000951"
     CDS             complement(34395..35222)
                     /locus_tag="ACHGNS_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFN6936914.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(35219..36286)
                     /locus_tag="ACHGNS_000952"
     CDS             complement(35219..36286)
                     /locus_tag="ACHGNS_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFN6936915.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(36312..37148)
                     /locus_tag="ACHGNS_000953"
     CDS             complement(36312..37148)
                     /locus_tag="ACHGNS_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6936916.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(37145..38014)
                     /locus_tag="ACHGNS_000954"
     CDS             complement(37145..38014)
                     /locus_tag="ACHGNS_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6936917.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(38084..39367)
                     /locus_tag="ACHGNS_000955"
     CDS             complement(38084..39367)
                     /locus_tag="ACHGNS_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN6936918.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(39381..41066)
                     /locus_tag="ACHGNS_000956"
     CDS             complement(39381..41066)
                     /locus_tag="ACHGNS_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFN6936919.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            41341..42366
                     /locus_tag="ACHGNS_000957"
     CDS             41341..42366
                     /locus_tag="ACHGNS_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN6936920.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
BASE COUNT        13265 a         8473 c         6898 g        14270 t
ORIGIN      
        1 ctatctgagc gatactcttt tataatactg aattcggaat taaaaaccac gataggtaac
       61 tttaaacaaa catgaagaga ctttaataag acttctttat ctgaaaacac ttttgtttct
      121 ctctttcctt tctacatcat taaatagtat tttatacgtt atctaaaata ctattcctct
      181 gtcaaaataa gctaacttac ttcattataa aatatccact tatcgctttc atgtatattc
      241 caccgtaaaa agtacatttt atcaccgaga actcatcatt tttcatcatg atgaatacta
      301 tttacatata tctagttcaa agacaataat tattatgatc cgttattatc taagcactat
      361 acagataata ggaatttttc gtttctaaat attccaattt gggaatccaa taaaaatttt
      421 cgttcaaata cgtgtctatt cgaactcttt tctagttgtt aattatgaag ggggaagaaa
      481 taaaaaatac aatctataca cgaataagaa tattatccaa aaggaggttt tagaaaagat
      541 ttattatacg taaaaagctc aagacatttt cttagccata aggacatgtt tgatatttaa
      601 gaagatatat gaattttctt ttctaagtat aaagataact atccattatc aactaaaaaa
      661 acatttttga aaataaacat cagcagtcct atcaatgcat gtgagcttat agaagttatt
      721 cgcagcgcag cataattgca ttagcttctt ttcgtgtttt ttgcctcttt tccctttaaa
      781 tcttaatgga ggaggtgtgc aatatgtcag ataacgacga acgcgtaaat gcccagttga
      841 ttcgattctt tgaaaaggtc attttgaaca ccgctaatac ttattataaa aaacaagaag
      901 aaattcaaaa acatgaacaa cttgatcaac tcccaccata tttgtttact aaagatacta
      961 taaaatttaa acaaccgatt aatgttttaa atgtgactct ccttgttgaa gatataaaat
     1021 tagcaaaaat ccttcctttg ttgaaagaac ctgaacaaat ttttcttatc gaaaaattta
     1081 tctttgataa aactgataaa gaaattggaa aggttctggg gattacaaga cagggtgcga
     1141 caaatttaaa acatcgactt tacaaaaaaa ttcaaaaaat gctggtttaa tatctgcata
     1201 ataactaatt ttgtatgctt ctttgctcgt tctcctcctt cccaaagaaa tagattataa
     1261 gtccatggga agaaggtaga tttatgaatt ctttaaaaga agatttcatt ttagcaaagg
     1321 ctggaaatga agaagctgtt gaggcaattc tcaaaagatt tagctctttg attcataagc
     1381 aatcctggag aactggaaaa tatgatcaag attgttatca agaatgtatg cttgcaatat
     1441 atctagctat ctcgaaattc gaaattaaag aataactcaa ctaagggagt ctgtgacata
     1501 agtaaaaaga tagtttcaaa tttcgaacaa taaatttgaa actatcttct ttataattat
     1561 aaagtatcga ttttacagcg ttctataatg attcatagaa tctaaaacga atgattttaa
     1621 ttatcaacgt tttttaattc gtttttagct taaaaataac ttttgtcaca gtctcactcc
     1681 taaaattact ttatttacgt atgaagtggg caggaggtcg aatccagaac tgggctatgg
     1741 tacttcaacg aggacttctc ctctagaatt gaaaagcatg cgatttacct tccgtaacga
     1801 cggtagattt attcaactat gtgtccaatc aaaatttgtc tgatttcaaa aatagtcaag
     1861 agaattttgg ttgttaaaaa ctcttatcta tcaacttcca cagtttagtt gacaaaccct
     1921 gatactaagt tgggctatga tacacttttc ttgactatca gttacgttac cggaaggaat
     1981 gaactctatt gagaaaaaca tatgatcgta aatttaaact aaagatttca caggatatcc
     2041 ttgagaaaaa agtaactacc aagaaaattg ctgaagaata caatatctct cgtccaacaa
     2101 tttcccgatg ggtttcagaa tatcgtcgat acgggaaaaa tgcttttgct ggacaaggaa
     2161 aaagattacc tgataaagca gatttctaca ttcttgaaca ggaaaataaa cgactaaaag
     2221 aggaagacga gtgttctacg aacacaaaga tcgatacgga tctccaggga tcactcaaaa
     2281 actttacgat gaaggaatcg aaatcaataa acgtgttgta gctgttctta tgcgtgaaat
     2341 gaatttatgc acaagaggat ttcatcgacg caaatcttct catggtagag aaaaaacaat
     2401 caaagaaatg gtaaaaaaat gttgaatcga taatttgaac agaaccaaat tgatgctgtt
     2461 tgggtgatgg atattactta cattccatgt aatgacggtc gcctctattt gtccacctat
     2521 attgacttag caactcgtat tctacgttac tatatggtag attcacacat gaaaaaagag
     2581 attgtcgtcc gtccattaca aatttattaa ggagaattgc ctacggtaat tcattccgat
     2641 agaggtagcc agcatcggtc ttttatttat caagagctgt tgaacgacca ccacatcaca
     2701 cattcagtga gccaaactgg gactcctgtt gacaatgcag taattgaagc gtttcatcga
     2761 tcaatcaaat gagaattaat cgactccaac aaatataaaa gtaaaataga gatgaaggtg
     2821 ttaatccaag attatttaga agactactac ccaaacaaaa gaatccatac aaaatttatg
     2881 atgactcctt gacaatttga agcgaagaaa ataagggatc tataatatct agtgttggtg
     2941 acgccttctg ttgccaatac tttttctttt ttctaataaa cgcaaacaga caccttaaaa
     3001 tcctttagaa taaagtcgac gaaaaccatt caaaggagtg tttcaagatg tcctattcag
     3061 agtttaccaa agagttacta gatattctag acctaaatct tatctttcat gaagatgcct
     3121 ttcgcaaaga gcgaatcaat gacgaaacat gttttgtttt tgatggcaca ttgacctatc
     3181 aaccggagga atgctttcat cgccactatc aaaatgaaca aacgatcatt aaatagggct
     3241 ggaaaaaagt atccatctta ttgaatgatg tcagtaacta caagaccatt ctgcggatta
     3301 acaaacagcg cttcaaatgt aaacactgtg gaaagacttt tttagcagaa gattcagtta
     3361 gtgatcgtcg ctgctccatt gctcgcagag tcaaacaagc cattcttgag ctattaagcg
     3421 aacctctttc tatgtctttg atcgcacgta tgaagcacat ttctccgacc actgtcattc
     3481 gaatacttcg cagccttcgg cctaaaacca tttctttgag ccagctgtta ccagaagtgg
     3541 tgtgtttcga tgaattcaaa tcagtgaaaa atgtctcggg agccatgagc tttgtcatga
     3601 tggacggcaa gacccatcaa ctgattgata ttgtggaaaa tcggcaactc aatcctttac
     3661 gcgactattt cttgcgctac cctataaaag tccgtgaaca agtgcgtcta gtggtttcag
     3721 atttctatac accctatcgc acattggtga aagagtgttt tccgaatgcc cgaatcgtcg
     3781 ccgatcgctt tcatattagc caacacattg gtcgggcttt taccaaccac cgaattcaag
     3841 taatgaagac ctttaaaaaa ggggatcgtc ggtcgaagca cttaaagaaa tactggaaac
     3901 tacttcaaaa gaatgcgtgg gaattgaaag gtcagcatcg ctactggcgt ccgtcatttc
     3961 gagatcatct gacagaagca gagatagtcg atcggctatt atcttatgat gattccttaa
     4021 agcgaggcta cgaagtctac caggattttc tttccgttat cagaagacaa gatgttcctg
     4081 aattcgtcgc gctcctaaaa gaagactaca aggagttgcc agagcactac caaccagtgt
     4141 tcacgacctt caagaaatac caaacagaaa tcaaacgagc gttgcgcgtt ccctattcca
     4201 acggccctat agaatgtttg aataaccata tcaaagtact aaaacgaatt gcctatggct
     4261 atagaaactt tcaaaactac cgagaacgaa tctttttata tcgaggaaaa tattttaaga
     4321 aaactaagaa tactacccaa ctgactaaag ccagaacaac aacacggcta gacaagatag
     4381 ctgtctagcc gtgtgagtat ctcgattatt ctattgaatt tcttcaccaa cactcattga
     4441 cgaagagcca aaataagtaa tctaaccgac tgttaactaa accattgcag gtcccgttaa
     4501 agatacttgt gcctgttgtc accgcttctt tttcttgtcc acaagcgctt aacacactta
     4561 ataaagcccc tgctattatt aataataata tctttttctt attaaaaacc tcctcatcta
     4621 tttccagatt agtaaatgta gtaatctgtg ctttcttttt tctcaatact aataactaac
     4681 ttatggggta aacaaatact cgtttgaccg ggttttgaga tccatccagt tctaaccgcg
     4741 atttgatcag gactattatc ttctttaact cgaattcttc catctttaat ctcaacaata
     4801 ttatattgtc ccttcgcagg atgataattc acgagtttat gattgatctt atccaaatcg
     4861 aagcgatcca tttccttgcc atcaattcga acgatggcat atttgatagc atcttcagtt
     4921 gtttcattct ccttttctat agagaataca aataagggaa taaaagaggt aataatcaaa
     4981 atgccaataa ttatgacatc gcctatcttg acaccagctt ttcttaaaaa tttcattaac
     5041 gatatctggc taaacatatt agactttttc catggaaatg gtgaaatttt tcggaaatta
     5101 tctgcattaa gagaatcagt taaacaaccc tcaagttgtt ccttaataca ttgttgatcc
     5161 atcttcatac caataaaaac tagtttatta atgcgttcac caaacaacgg atgccaggct
     5221 tccttcaatt cgggattttt ttgtataatc atttgacact caattttagg taaactagct
     5281 acccaataat ttaatgattc aatactttta tagctacccg atgtttcaaa gatcgtcgaa
     5341 aaatcactat gttgggcaaa ccaaagaaac ccttttgctc ttgtaatatt gccaggaaaa
     5401 tttttatccg taagatgggc aaatttttgt gcgtcaagga gtaaccgatt tcgatagata
     5461 aaacttgaga taccatattc ttctgtttca ggaatatgat tttgatagcc aagctctaac
     5521 tctttgaacc agcccactga ataggaagtc tcatctatat taaaaagatt tgtatttaga
     5581 attttcgata agtctacttc acctttaatt gtcctaatta ttttggcatt ggactgtaac
     5641 ttagcaatta gaccgttaat tttgtaaatc gtcttggttg acactaagtc actattattt
     5701 aaaagtaata tgttacaaca ctcgaattga tcaattaaca agccacgaat atcctgttct
     5761 tcttcaacta cattaccttc agcttttctt tcctgaatct tttcacccgt ttcgaaatta
     5821 tcccaaaaac gttttacatc cactactata ataatggcac caatctctaa tcgttgggtc
     5881 aaatcaatgt ctaaagggtt tttagctaat ataattgctt gaataattgg aattggtttg
     5941 ctgattcctg aagcttcaat aacaatttga ttcaagtcag aaaattgcac taattgatct
     6001 agttcaacaa tcaaatcttc gcgtaatgta caacaaatgc atccattcgg catcgaaaca
     6061 agtttttctc cagttctttt aaaacctttc ttgtcgacta ataccccatc aacatttact
     6121 tcccctatgt catttacaat gacagcaatt ttctttccgt gatcaccgta cagaatatga
     6181 tttagaagta tcgtttttcc tgaaccaaga tagccagtta agattgtaat aggtattttt
     6241 ttcataggga acctctttct ataccttgaa tttacacttt aagtgcaaca cataaaaatg
     6301 agtcataatc gatatccaac tagtataagt ttgtcgagaa cactcctaga aggaaaataa
     6361 ttatgagcca gtatcgtcat cttactatac tcgaacgaga aagaatcttt ctgcttcacg
     6421 aagaaggatt ttctattagt ataattggca acgacattca gcatagcccg tttactattt
     6481 tacatgaact gagaagaaac aagaaaaaaa ctattcacct tccaaagcgg aaaaaaatta
     6541 tgcttatcga agaaaaaaat gcggacgtta tcacattcta aatgatccgt gagtttggtc
     6601 aattgttcgt catttatttg tcgattttca gtggtcgcca gaggaaatct cgaatcgttt
     6661 gaagcatgaa catatggact tttcaattag ttttatcacg atctaacgtg caatttgcaa
     6721 tgatcttttc gatacagaac ccctctcaca cggcaataaa ggcctgatcc gttcattgca
     6781 ccatcgagga aaaacaagac atacaaaaaa tcatgtcgag tctcgtggaa agatttgcct
     6841 tactcattcg atccatggcc gtccaaaatc cgcagatgat agaagtgatt ttggtcattg
     6901 gaaagccgat attgtagctg gcaaaactgg tggtacttgt ttagtgactt tgacagacag
     6961 aaaatcatgc ttccttttag caggaaagaa ggtccccaga aagaaagccg cttatgtgag
     7021 cgagaaaatg attgaacttt taagcagtat tccaaatgag gcggtcaaaa cgatcactcc
     7081 agatagagga aaagaattct ccaatcattt cgcagttaca gaagcattga ataatatgcc
     7141 cttttatttt ccagatcctc atgcccttgg caacaatgaa caaattaaaa tacgaataga
     7201 ctcattcgag aatacttgcc aaaatcgaaa gagatagcta cggtagataa ctcaacgata
     7261 aatcagatga ttgaaaaact gaataacaga cctagaaagt gtttagattg gagaacacct
     7321 tatgaagtct tctttgataa agtgttgcgc ttgatttgac aattcaagac ataataaaaa
     7381 gcagatcaaa tccaaataag ggaaaaatga tctactctgg tattatcaat taattggcca
     7441 agctattatg ccgttttgct aaacgtctac ttcttccagt attgaccttg ataaaacaac
     7501 aatcaggatc ccgttttaat actctatgaa ctgtgtcaat actaacatgt aaagttgcag
     7561 caatatcagc taatgtactc ccatttaatc tcatttgctt tgcaaatcga taacgccgat
     7621 aagcatcaat attcttggaa tccatagaca ataatacttt tcttaccatg tatttcctcc
     7681 aaaaattgaa ttttaatgag ccatcatttc ttgagcaatt tcatgtttag acaatgaagc
     7741 tggatagaaa gtaggccaat tatgcaattc attatagagt ttttcttgac tttctccacc
     7801 aaagaaaata tggaaatgct ctgccttact tggtgcaatt ccatgatcac taaattgaat
     7861 atatttaaaa cgaccagcac ctgcatcatc agtttcaaaa ttaaaacgaa caccacggtt
     7921 tcctttttca taattcaaaa ttttgtatcc aacatatttg tatgtgtatt gatgatgttc
     7981 accgttgact acaaaatcaa tggtgttgtc tttaatgttg atagtatcaa tatctgtctt
     8041 atagccagta gtataataat ctttgtactc ggcagctgtc atgtctttat tgatttttgc
     8101 tttgtaatcg aaaacttcat ccaaaattcc atcttttaac aacggataaa cagattgcca
     8161 ttcgcctgta taatcactca attcgcgatc cttcacatcg gcatctttaa agtacccatt
     8221 agcaactgtc ttttcatcct tcgcttcttt ttctggaact acctcttcac cagcaaccat
     8281 agttgttttt tctaacgctt ttagattgtc ttccattact gaaacataat cttcaccatt
     8341 atccatttgt tcttttgtaa gactttcaag aggattcagt acttccaatt ttatcccagc
     8401 ttcgttggcc aaagtttttg caattttatc attagcattc ttttcaaagt aaatataatt
     8461 gatcttattt tttttaacat actcttttaa ttcagctaat cgaccagaag atggctcttc
     8521 ttcaggactt agtccagcaa taggaacttg tttcaatcca taatccaaag ctaaatatcc
     8581 aaaggctgcg tgttgcgtca caaaactttt ttgtttcgct tctttaagac tagtggtata
     8641 gtcagcatcc aaccgtttca gtttagtcaa atacttttca gcatttgtct caaatacttt
     8701 tgcttttttc ggatacaatt taactaactg atccttgata tttgaaactt ccttaattgc
     8761 cattttgggt gaaacccacg tatgaggatc tagttcatga tggtgatctt cttctccgtg
     8821 atcatgatct tcttcttcac tacctggcaa taaaatcatg tcttttgttc cttctactac
     8881 attaggccca gctttcttcc aactttcttt agcttttggt acccatgatt ccatgttttc
     8941 gttatgatat acaaaaacat cagcatcatg agcttttgcc atatccttag ctgagggctc
     9001 atagtcatga ggttctgatc ccgctggaac cattaaatcc acgttcgctt catcgccgac
     9061 gatgtttttg gtgaaatcat acattggata gaatgttgtt acaatttcta attttccatc
     9121 tttatcattc gatacttttg catcattagt gctggagcaa gctcccaata atcctagagc
     9181 tgctatccca ataagtccta cactgatttt cttcattctt tttccttcct taattatgct
     9241 aatttcttca ttttatttcg tttatgttct ttgataattt ttaatgccct ttttctagtt
     9301 ttaatattcg ggcttttaag tcgtcgatat gcactagata tatccgtttt ctccattaga
     9361 aattaccccc aatcactact tatcattatt tgctttccgc cagtaattta tcaaaactct
     9421 gtttgatcct ttcttcatcc aacatttttc cgattagaat aatagtggtt tggtcttttt
     9481 gtcgagtggt gtattgaaaa ttaacctgtt cgtttacctc ttgcatggca actataaaat
     9541 cctgctcttg taaagcaatc aatcctttaa aacgatacag gctcccttgg ttggttaaaa
     9601 tcagccagtc aatccattgg agtaaaaaat ctttttttat gtcttgatct gaagttaaga
     9661 gaattgattt aaactcatgt acttgatggt gttgatgata gtatttgcca ttatagtgat
     9721 tttctgatga atcactttca aaaactaagg gatgattaaa ttttttcaaa ttataaaaat
     9781 cctttttttg aatcgtttca ctatgtgtga acattcgaat atcggataaa gggttaatac
     9841 ttttgatttc tctctgtaat ttttttagtg cagataaatt caagttttgt tttctagaca
     9901 caaaaatgcg atctgccatg acaagttgct taagggcttg tggttctctt tcaataagtt
     9961 caaaactgtc actatcaata acagtcaata aactatcgat atatactctt cctcttagtt
    10021 gaggcgctgt taaaagtgtt tgaatgattg gttggggatc tgctatacct gtagtttcaa
    10081 tgatgatttg tgaaattggt tgttgatgtt cttctattgt ttccagtaaa gaaatcaata
    10141 ttgctaataa atcactgcga agactgcaac acatacatcc attatttagt tgtaacaccc
    10201 gttcctcgct atgaactaat aatttgtgat caatcccaac ttcaccaaac tcattttcaa
    10261 ttataataat ttcttccctg gggtagggtg agacttgcaa ggcttgattg attagcgttg
    10321 tttttccagc ccctaaaaac ccagaaacga ttgtaactgg tatgcccatt ttgtcacctt
    10381 tttaccactt agctatttca cttctttaaa aattgcccgt ctgcgcaatt ttggcgaata
    10441 cttttttaat tctaaccgtt cagggttatt ccgtttattt ttgctggtga gatacagacg
    10501 ttctccagtt tcgacacatt ccaaaataat attttgacgc ataaatcttc ctccttcaat
    10561 acttatcgat cttcaagata tgcttgaatt tgttgtgcat cttcaaaagt taactcgcca
    10621 gattccaatt gtgaaactaa attttctttc atatcaacag gtatctccca ataaccatcg
    10681 atacattgaa ataactcctc gtcatactct tcaaaaattg gatagattct taaaaaatca
    10741 tcaaatgata agccttggtc ctcatagtaa tctttgacta atccattttc aattgcctgt
    10801 tgggtcggta aacccgtttc agtaataatt cctctctcta ccatcgaacg ataaaaagtg
    10861 catttatcta gcgattgctg ggtcgtttca agataatact ttaatccttc ttcaactaaa
    10921 ggtgggtaaa taatttccat tttgattccc tcctaaaagt tatatattaa actattttga
    10981 agctccttta attactttct ttccattata gtaccctttt aaagatacat gatggctacg
    11041 tctataatca ccattcgctt cgtcaaatga aatttctggt tttactacag tttgatgagt
    11101 tcttcttaac cgtttcttcg ccttagatgt ttttcttgct ggtactgcca tgatatttcc
    11161 tccttttacc aacttgcttt ttttactcca ggaatcaacc cttgatgggc aagttctcta
    11221 aacttaatac gggacatacc gaactttcgc atataccctc ttggacgacc atccgtctga
    11281 tcacgaagcc tcagacgatt cggatttgaa tctcttggca atttagcgag agcggtgcga
    11341 tctcctgcgg cttttaattc ctgacgttta tctgcatact tttcaatcat cgccatttgt
    11401 ttctttgctt tagcaatttt cgactttttt gccatatacc tttatactcc ttttattttg
    11461 atttagacac ctcaaatcgt aatcgttacg ttttgacact gatactttac caacaagagt
    11521 tgctcttgtc aacaataaat aacatgaaat aaaactcata tgaatagata actatttgaa
    11581 aacgaattta tcggtacata gagataatca aatcatttaa aagaatatgc agtttaggct
    11641 ctgaaaaata agaattcact tttattagtc caatcaaaca caaataccgt tttaagtagc
    11701 ttttcctcta cttaaaacgg caatatgaaa gaaactataa ctttttattc aatcttttat
    11761 gtttaactgg tttatcgcca attttttaaa actttgatta ttccttatat tataagaata
    11821 gacattaagt gaactaattc aatcatactg attctttcgt gtattcaaaa ttagatgctg
    11881 tttaataaaa ttattctttt tgaaaaagaa ttataactat atcccaaaaa ttgtaggtta
    11941 atttaaagcc tatttcatca tcgtgattga gcttcttttt catagacgaa tttctcagat
    12001 agtttagttt tctttatgag atttttgtat atttctgacc ttcgatacaa ctcattatga
    12061 gtaccttgat caactagttt tccttgatcc atcactacaa ttttatttac cttttcaata
    12121 gacttcaagc gatgcgatat aattagtact gtcttatctt gtattaaacg atttaaactt
    12181 tcctgaatac gtttttcatt ttccgcatct aaactggcag taatttcatc taaaattaaa
    12241 attggcgcat ttttcaaaaa agcccgtgct atggataggc gttgtcgctc tcctccagaa
    12301 agtgaacttc cgttttctcc gaccaatgta tgataaccgt caggtagttt ttcaataaag
    12361 tcttcgcagt tagctaaacg agctgctttt ttcacttcct catctgacgc ttgactattt
    12421 ccaatacgta tattttccaa aatacttgta ttaaacaaag ttacctcttg aaaaacaata
    12481 gcgattttgc taaacaaaga agctggagat acacgtttaa tatcataccc atcaattaaa
    12541 atacagcctt catcataatc atacagtcta gacactaatt tcaagatact agttttcccg
    12601 gaaccactag cacctactaa cgcagtcact tctccttgtt tagccgtaaa agagatatga
    12661 tctaaaattg gggtattgtt atcatatgag aaagagacat ctcgcaactc aatgtcaaaa
    12721 gatttcagag gactatctga tccctcttgg atactggttt ccttcatagc tctaattcgc
    12781 tgtattttag gagctaaata aaatatttca agcactgctt ctttcataga atcgaaggaa
    12841 tcctttattt ttattgcggc tagtaagtaa ccaacgacgt agagtatagt cacttcaccc
    12901 gttaacagta agtgaacacc tactagaatg actactgcta aactaacata tgagaaaata
    12961 gaggataagg ccatgactga aaaagtactt aattctacct ttaaatgaat cctttcactc
    13021 tcttccattt ttttgaaaag tctgtcttga acttttttag atagattaaa gctattaatc
    13081 tcttgctgca attctatagt ttcttgaaat tcttccgagt tctctctcaa agtatcgtaa
    13141 tatttagtat tggctttcgt ttgggatttc tttgataaca agattaagac aaaactaaat
    13201 aacgttggca aaataacagc taatcccatt ttgacattac caaccaaaag catcactgaa
    13261 ataaaaggga aaaacagagc cataccgccg gctttaggta ttgcatgact catcgcatgc
    13321 tcaatacctt caacgtcaga catgatagtt tgagataaat ctgataaatt atgttttgaa
    13381 aaataggata atggtaaatt tgacaaatcg tctgcaattt gcactcttaa atgcgcactt
    13441 tctttataag tactattata taagttttca tactccctat ctaacaacca atacatgaaa
    13501 agcaaagtaa caaacgatac ggcaaaataa agccaacggg gctttgtatt ttctaaaact
    13561 aattcgtcta tcagcatcat tataatgaat gctggtacca tattaatgca gtatacaaaa
    13621 aaactagaaa tagaagcttt agttaaagct tctgcgccct gatcacttaa agcaaatttg
    13681 tttttataaa aacttttcat tgtcaagcct ccaatcattt gcattctcat aattagtgac
    13741 caattgttta tacacttgat gtttagccat taattcatta tgatttccac gttctactat
    13801 tttcccttct gacaagacaa gaatttcatc tacagattta atgctggata atcgatgagc
    13861 tatcataata actgtttttt ctttcatcag atgttgaaaa gccttttgta gttcatactc
    13921 attatctgga tcaatagatg cactggcttc atccataata acaatctttg catcttttaa
    13981 aattgctctg gcaatagcta tacgttgttt ttctcctcct gacagataaa ctcctttact
    14041 accaattaag gtatgctctc tttcgggaaa tttttctaaa atcgattcgc aaccggctaa
    14101 tcttagcgcg tcaaatactt tacttgctgg cgcttgagga tttgctatag aaacgttatc
    14161 gtaaatactc tgattgaata gtttcgtatc ttgaaagaca aaagcaattg tgttaataat
    14221 tgtgtctttt gaatagtgat ttaaaggctt accaccaatt ttaatctgtc ccttatccac
    14281 attgtagaaa cctgaaatta gtttagctat cgtactcttt ccgcttcctg attttcctac
    14341 taatgcataa attctttttt gttctaaaac taagttgaaa ttttctaaaa cacactcttc
    14401 tttgtatgaa aagctgacat tttcaaatgc aatgtcacaa tttttaatct gtgtctcgtt
    14461 cccatatttt agttggtctt gcttcatttc taaataaagg gagtctagtg tttcgactgc
    14521 atatcttgct tcaaacaagt gcatacttac atacattatt ctcatgaaag aaacaaaaat
    14581 aattccactt aaaaaaaaca acatcaacaa gtcaacagct aatatcttgg gagtggtaag
    14641 agaagttata aaaaaggtta taggaactat caaaatgcaa actatgccaa aaaatagcca
    14701 ttgaaaaaga acaaaaggtg ttttcccact caaagaatac ttatatgcaa acttagcata
    14761 atcgttaatt gctttattca aggctttaaa tgatgttact ttagcaccaa atatctttat
    14821 aacttgaatt ccgcggatat attctaccgt ttcagagctt aacacatcta acgattcttg
    14881 atatttttga ataaaatttg tattctctgt cattttcttt aacaagaata caccaattac
    14941 taacatggcg attagaacca atccaatttt aaagttaatg aaaaagccta aggcaactga
    15001 taaaacaggt gttactatgg cttgagcact atctggaatc aagtgagcaa ttgccatatg
    15061 tgttttagcc gcgttgtcat caattgtctt tcgaatatat cccgaagaat ttaaatcaaa
    15121 aaaacgaaaa cttgcttctg ttaaaccgct aattcccttt tttctcaaat tagtctctaa
    15181 gcgaaaccca aggatatgag aaaataagcc agaaaaaata taaaataaag ctcctaaagt
    15241 taaccaagca acagtttgaa ccgcataact cttagctaaa tattcatctc ctacaataat
    15301 tagagactga agaaatttgt aaatactgta atatccatag actgttaata cgacagaaaa
    15361 atcagaaaat aaagtggcta accatccgta gtatttcatt ttagggacat aactaaataa
    15421 ttttttgtat actttcattt tttcctcccc cgcattcaat ttcttctctt attcgtttac
    15481 aactgttaat gctttccgta atagcgtaaa ttagagatgt acaaacagat aaaaggtaaa
    15541 ctacgttcta ttttttgtat ttttttaaga cttttcttcc aatcaataca ccaatcatag
    15601 ataaacttgc tacaactaac actgaaatga ttccccatat acctaccgca tagtggatca
    15661 ttgcatccat tgttgcagca gattgtcctt tatctatcca atcttgacga aaacgatctg
    15721 taaagaacca tataggaatc cactgtccgg cagcactgcc taattgcatt agagcccaag
    15781 acgcaacttg aggaattgct cgatcatatc caaatacaga tagaataata tcagctaaga
    15841 tcgccatact aatcgaactg ataaaccaag gtaagtacgc cccgcccatt aacgtaaaaa
    15901 ataacaataa aacaattgtg taaatagtaa aaattccttt tctagggtag ttatagctaa
    15961 tataaacaaa tatgattcca gataataatc caaaaatccc gggggatatg ctgtgaccaa
    16021 acgccccacc aatactacta accattattg aaaaaaagta aattactagg gaaataactg
    16081 tagctaaacc aataaaaata taatcttttg ttttcatata tattctcctc aattctattt
    16141 cgttatgtca ttaaaaatct gttctatcaa agttgaatct tctactctaa gttttatatc
    16201 tttttttatt ccaccatttt ttaaataaat aattctattg catgttttat aaagaaattc
    16261 cggatcatga gtagctataa ctactgcacc cttttctttt aagtctttaa taaggtcagc
    16321 aatcctttcc atattaatat aatccaaccc acttgtaggt tcatcaaata gaaataaatt
    16381 agtttttgac aatgaagcta tagaaaccaa taatctttgt ttttctccac cggataaatc
    16441 aaacggtaat tgatctttca ttttataaag ttccattttt tttaaaattt tttcttgctg
    16501 atctttacta actatttcat tccccagggc taactcgctc ctcactgatt ctgtgaaaaa
    16561 ttggtaatcc atttcttgca ttactaaaaa cggcgatgac aaattttttt tattttcgat
    16621 tatctttcct ttggtagggc gaataatctt catgagtatt cttagtaagg ttgttttccc
    16681 cacaccgttt ttccctacca aaccaataac atctccaact ttgacatcaa aatttatgtc
    16741 ttttagaata ttttcataag taatttcttc aacttttaat acatctgcac ttaccttttc
    16801 gatataggga acttcaatcg aactattaaa aatgtcaaat ttccttaatg cgttattgtt
    16861 ttcagttttt aacaactgaa tagttgtagt ttttacagtt ctatttttaa caaatagaac
    16921 ttgatcaatt aattctttca aatagtaaaa acgatgttca gctataacaa tagtaaatcc
    16981 tttatttttt aaatcacaaa gtaagtcagc aagtttctta gtcgctttga agtccaaatt
    17041 tgaagacggt tcatccaata taattaattt agagtttagc gataaactac atccaataga
    17101 aattttctgc cgttcaccac ttgatagcga aaaaatgttt ttatctaata tatttgaaag
    17161 atctaataat tcagctaatt cattgattct tttatccata tgctcttttg taaatccaaa
    17221 gttttccatc gaaaatacta actcactcgt tgtatttgtt gtaaagaatt gtcctctagg
    17281 atcttgactt acgtagccaa tattttttga tatactttct tgtgttaagg attgtagcga
    17341 gttattttca aataaaattt ccccaaccaa ttttccgtca taaagtcttg gaataagtcc
    17401 attcattaac tttaatagtg tggatttccc actcccagaa gaaccagtaa tcaatgtaag
    17461 actgcctttt tttattgaaa aattaaggtc aacaagcgct tgttccttaa catccttata
    17521 tgaaaaagaa acattgttaa attcaaacaa ttctttcacc tcaaatcaaa atacttaaga
    17581 aaaaaatagt aacggaaaac aggatgatat agtctaataa gctcatgttt gttttatagt
    17641 aagaacttct tttcacagga gattctatcc cctttgttat tgcattagcg gtcatttctt
    17701 cagcaatcat agttgcacgt ataagaacgg gaactaacag aaattccaaa tattgcagag
    17761 gctgaaaaaa agacaagcga atgtttctca tttttaaaga ttctttaatc attcttattt
    17821 cagaacgata agtcggaaag aatctgatca ctactgtaat acttaacatt atttttttcg
    17881 gtaaatgtat tttttctaaa gaggataata attcattgct agtatagaca ctaaccaaca
    17941 accatgagat cattacgacc ggcaacattc gaaatacaat atataaggat gtcgcaaaaa
    18001 atatagtaaa aaactctatc ttgatagcat caataatttt cattactaaa aacagaactg
    18061 aataaatcaa gatgaaataa atacatgtct ttattttacg aaacatcaat actaaaactg
    18121 tagcaataat gaaacacccc acttgaaata tatttccctt tcctaaaaaa ataatgaccg
    18181 gaaaaaaaat atttaaagta atcaaggtta acggattgat ttttgacact ctttcacctc
    18241 caaaaaataa gatcgcactg ttatcttaag ctgataaaaa tgcaccgtta ttcagataac
    18301 agtgcgatct tatcaaaaag cataactgag aacaattctc aatgcaagct ttttttaatt
    18361 aactcagtaa ttcaatccac ccaggaacag aaaattgata caatcttctg gttaatagca
    18421 tagtcttttc ttttgaatag ctatgcttca atatatcata gaatgtagcg tagaatgagt
    18481 aaatgtagaa gtggatttct tcttcattaa agactaaatt ttgattgatc attccgatag
    18541 tctttaactt atctagacaa cagactgtct ttttcgtcac aaatgatgta attttttcaa
    18601 caatatcttg atatttggtt ccagctgatt ggaataaaat caaatcgaat atacgtttat
    18661 tctcaaaaat ataattaata aatggatcta attcattgaa gttctctctc caacttttct
    18721 ctatatctat attttctaag tttaagaggt atttggtaaa ctcttcatta tacatattaa
    18781 taagcgcatt aatatgcggc tgaataattt cttcaaataa cgcttcctta gaagtaaaat
    18841 gtttataaaa agcacccgta gttacgcctg cggtcaggca cagttgcctt atcttagttt
    18901 gctgaaatcc ttctttttca aaatggctta gtccactttc tattaacttt tggtgagtta
    18961 taatataatc tctggtttct atgggtactt cctcctaata agatgaatta actttattat
    19021 cttacacagg acttgaagtt tcaatcgtta acgtttatta ctgaactcat tccttagagc
    19081 taaactaatg agaaagagca gaataactga tacaaaaagc aatcctacaa gaattaatag
    19141 actcatctaa atcattcacc ttttctaaaa taatccgaga aagatttaaa aaaattatat
    19201 ccagctatta ggataagtcc tatcataatc actccaatag ccattttctt atcctcccta
    19261 aaaataaagt cttttactta aaaccttttt tgtaatcgtc aataaatttt ttccctttat
    19321 cagttaattg aaatcctata tctaaatagt cctcatatct aagtttagcc agcatctcac
    19381 ttacagatgc tggactaaca cttagaactt ttgctaaatc attatttttt aatttctttt
    19441 ttctttttga taattcctga attgctagta aataaattag tatagtagtc gttttaattt
    19501 gtaccatacc taccctccaa attatttcga aaggccttca gcaattttat ccaggttcca
    19561 tttcatcatc gcatagtaac tatcaccatc ttgtcctttt tctgcaattg aatcagtaaa
    19621 aatcgttgaa tagataggaa tattggtatc ttttgatact gttttcattg gtctatcatc
    19681 cacactgctt tctacgaata aggagggaac ttttgttgtg cgtaattttt caactaagtg
    19741 ttttatttga tctggtgttc cttcttcttc agtgttgatt tcccaaatgt aagcagaagg
    19801 cacattatac gctttcgaga aatatttaaa gcatccttca cttgtaacaa tcattttttt
    19861 atcattcgga attgaagcaa atttagattt agcttctttg tctagagaat ccaatttttc
    19921 aatatactta tctagatttt ctttatagaa ttttttatta tcaggatctt tttccgctaa
    19981 ctgtttttca atatttttag cgtaaataat accgttttct aaatttaacc aagcatgagg
    20041 atcttccttc cctttctcac tctgaccctc taagtaaata acatctatgc catcacttgc
    20101 tgcaaaatag tctttgtttt cctctttgtt cgcattttta actaattttg taaaccaagc
    20161 atttcctcca gtttccaagt taacaccgtt ataaaaaatc aaatctgctt ttgaagtttt
    20221 ttgaacatct tcaggcaaag gttcatattc gtggggatct ttcccaatag gtacgatact
    20281 gtgtaaatca attttatctt ttgctatatt ttcggtaata tctgctaaaa tcgaattagt
    20341 aactactact tttaattttt cgttcgaagc cactttatct ttgtctttac tatttattga
    20401 acatgccccg aatataaaaa gagcaatcaa aatccccaga cttcccagta ctgctttcca
    20461 ttttctcatc ataacgatct ctccttcttt cttaaaaatt tttgttttgg tgagattaga
    20521 aaactaatta caaataagaa tgccgctgtt aaaacaatac ttgatcctgc tgctatgtta
    20581 aaactataac caataaacaa tccaagcaat gatgaacttg ctccgactgt tgcagaaata
    20641 aatatcatag ttttcaagct atttgcatat aaatatgctg tagctgctgg tgtaattaac
    20701 attgcaacaa ttaatatagt tcccacactt tgcatagctg ttactgctac taaagttaat
    20761 agaaccatca atagataatg ataaaaactg acattcatcc ctattgcttt tgccattaat
    20821 ggatcaaaag aagtgattaa taattcttta aaaaatagaa ctactataac caacactaaa
    20881 accgaaacgc caattgtaat ccacttatca atatcttgta cagctaaaat atttccaaat
    20941 aaaatatgaa ataaatccgt tgagctattt gcaactccta taaggataac tcctagtgct
    21001 aaaaaagaac tgaaagtaat accaattgct gtatctcctt ttattacact attgcttttt
    21061 ataaaagtaa taatgataga agctaataga ccaaatacaa tcgcaccaat aaaaaaatta
    21121 atacctaaaa tataagaaat tgctactcct ggcaaaacag catgagaaat agcatcaccc
    21181 ataagagaca ttcctcttaa aataataaaa caacctactg ttccagaaac aatccctata
    21241 actacagatg taattagagc attctgtaaa aagtgaaagt catacagacc attaataaaa
    21301 ttttgaagca tattattccc cctctccaat aaatatagta tctccatagg ctttttttaa
    21361 attttcttca ttaaatacag attctacact tccgtgagca attagatttc gattcaaaag
    21421 aataatatta tcaaaatact tctttacctt gcttaaatca tgatgcacaa ttagcactgt
    21481 ttttccctga tgtttcaatt tttttaacgt atccataatt attcgttcac taactaagtc
    21541 tatccctaca aatggttcat ctaaaaatat gaaatccgca ttttgagcaa gacatcttgc
    21601 taaaagtaca cgttgaaatt gtcctccaga taattctcct atttgatgat tagaatattt
    21661 tcccatatcg accttttcca aagcctttga aactctttgc cattctgcat ttttaattct
    21721 ttgaaaaact ttcattccgg catatgttcc catagataca cattctttta cttttattgg
    21781 aaaagtaaaa tcaatatcag ctttttgctc tacatatgca atcttcacca gttttttatt
    21841 gatatcttca ttattaatgg agacctctcc ctgatgaggg ataatattta acattgcttt
    21901 aagcaatgtg gattttccag caccatttgg accaataata cctgttaaag caccttcttg
    21961 tatttttaaa gaaatctcgt tcaatgctaa aaaatcgtta taacaaacat taagcttttt
    22021 cacttctaac ataactttct ccttctaaat gataatgatt atcacgatca tatggtatac
    22081 aaaaattata ttttagtcaa ccctaaattt tatagtttag atttttttct gttgctgaag
    22141 attagaatga caaaactatt tgaaactacg cgtatagtaa gtagctttca aaatatataa
    22201 aaaaggagcg ttctcaaatg agaacatgct cctaaaacta ctttattggc gtatggaaaa
    22261 attatacttg aagtggatag cgggttgaat aagttgataa gcatcaaaac atttataaaa
    22321 attattcaag actatgtctg ttccatatac aaatatgaaa atcagtaaac tccatttaac
    22381 ttttttcgta gtgtgtcttt ttatacatgt ttatcgttat taatagtgaa taggaaaaaa
    22441 actatttttg atcattgaca attaaataca atttttgaat aacgatccct gctattcgag
    22501 ctagaaactc aagtacgcca tgacctgatt agcgacaaat acttagagat ttctttttgt
    22561 tctgggaaaa caaaatgcca tgacaatagc agcaaataat gatacttccg taaggctcgc
    22621 cgtaaactgg ggagaggtga aattcctatg tgcttaccca aagcaattca aaaatttcat
    22681 aactactatt ttaggagatg attttataga atccagagtc tacctgaata aaaaacaaac
    22741 ctgtgcattt ctaggaatcg ataataatac tctagataag tggattcgta tatataattt
    22801 cccagtaata agagttggtg gtgtagttcg ctttgacgta gaagatatta aagactttat
    22861 gaatacattt aaaataactt gaaattttct tctcgccgca tatactaaaa ttagtgaaat
    22921 atactgcctt acttttttta aggagtgagg attttgccta caaagctttc aaatgggaaa
    22981 tataaaacta atctgagata tccaaaaagg tttaaagaaa taacaggaat tgcatcagaa
    23041 aagtatcaaa aaacatttcc aaataggcaa ttggcgatta aagccgaaaa tgatatgaag
    23101 aagaaaatag aaaaagtttt gagagaggag aatgcaaact cattagaatt aaaaggaaaa
    23161 ataacgttta aaaaatttta cgaatctaaa tggcttccta gatatgaatt aggtcaaaca
    23221 attagatcaa acaggcctcc tagtgatata accattagta atacaaaaga tatatttcgg
    23281 ttgcatattt tacctatgtt tggtgaatac gcaatgaatt atctaaatat caatactgag
    23341 ataatatcag atgaattaac caaaaaatcg aaagaatatg caaatataaa aatcataaaa
    23401 ggatatgttc gttccatgtt cgatattgca gaaatactaa attatatcga atttaatcga
    23461 actactaaaa ttattcaaag tatcactgca cctaaaaaaa atgctttaga agaaaaaaga
    23521 atacaagaag gtaagcaagc actatcatca aaagaactta ctaattggat agaagcagta
    23581 aatgatgatt ttaataatca tttacttact tttcatgatt acacactttt tatgattacc
    23641 ctgtatcttg gtgatagaaa aagtgagact tacgctttgc aatggaaata tattgatttt
    23701 gaaaaacaaa cggtccgtct taaacatact ttagataaat atcaaagaaa aaagtttaca
    23761 aaaggtcgaa aagataccgt cattcaagta ccagaagttg taatgacatt gctaagtgaa
    23821 tggaaatcgg tacaagctga tcaattgttg aaattaaaaa ttaagcaaac gttagatcag
    23881 tatctcttta cttatactaa accatctgga gaagtaaatt gtcctgttca tgccgattac
    23941 ctaaactatc gaatcaattc aattaaaaga aggcatcctg acttagttca tttatctcca
    24001 cacaaactcc gtcatactta tgcaactata gctagacaag gtggagcaga tatgaatcaa
    24061 atttcaaatg ctttaacaca ctcagatatt tctacaacaa agatatatgt gaacacacca
    24121 gatatagttg ataaggctgt ttttgaagct tttcagagag gattgaataa gtgtgattga
    24181 tatgtgttct taaatgtgtt cttaaaaaca aaagaagcac tttccaaata attgaaaagt
    24241 gccagaaacg ttgatatata aaggatatta acgttttgag aattgtgaag ctttacgtgc
    24301 tttcttaaga cctggttttt tacgttcgac catacgtgcg tcacgagtaa gtaagccagc
    24361 acgtttaaga gctaaacgga agtcaggatc aacttctagc aatgcacgag cgataccatg
    24421 acggatagct cctgattgtc cagcgtatcc tccgccgttt acgttaacaa atacgtcata
    24481 tgcgcctttt gtttcagtaa cagcgaaagg ttgattgatt acttcacgca agtcagcatg
    24541 tggaatgtat tcttcaacat cttttttatt tacagtaatt ttgccagttc ctggtactag
    24601 acgtacgcga gcaactgcgt ttttacgacg gcctgtgccg atatattgaa cttgtgccaa
    24661 tgaaattccc tcctattaga ttaagtttgt gatgtctaaa acttctggtt gttgtgctgc
    24721 atgtggatgt tctgctccac cgtatacgtt caattttgta aattgtttgc ggcctaaagt
    24781 gtttttagga agcatacctt ttacagaagt ttcgatcaaa cgacgagaat ttttagcacg
    24841 caattcgcca gcagtgattg atttcaatcc tcctgggtag ttgctgtgac ggtaataaat
    24901 tttgtcagtc gcttttttac ctgttaattt cactttgtct gcattgatga cgatgacgaa
    24961 atcgccagta tccaaatgag gtgtgaaagt tggtttattt ttaccacgta gtacagatgc
    25021 aacaactgtt gaaagacgtc ccaatggaac atcagttgcg tctactacat accatttacg
    25081 ttctacttcg cctggtttgg ccatatatgt tgtacgcacg ttattttcct ccaatttaag
    25141 ttctgatgtt tgacatacac ttcgagtttc cggggctctt tgtggggcaa acaataccat
    25201 ttaacatatt acccgctaat ctagtcaatg tcaacgtttt tctgcttgat tgaaaaattt
    25261 atatgaaact ttacttcttt tctttctttt atataaaagg gtcgtttcct gtcaaaatga
    25321 aaaacaaaaa gaggctgtga caaaagtctt gtcacaaccc cttattttga ataaacggtg
    25381 ttctcctgcc aactcctcgg tcacaagtca caatatccat aaaacataag aagctgtttt
    25441 ttaaaattgt ttcttgtgac tcggagctga acggcaggct tacaacctct tggtaaacgg
    25501 tgttcaatca gctgaccctc ggtattagct caaaaaacgg caaaatatga ggaactattt
    25561 tgacgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc tttttcggtc
    25621 aagttcaaca acagttttta cgcgtcgatc tctggcgctt tgcccaaatg agcttggatc
    25681 atccagatac gtttttctgt ggctgttttg aattcgataa agatatcttg tgttggatcg
    25741 tcgccttctt ctccagaaac ttcgattcct ttttggtaaa gatctgctag ataacgatag
    25801 cctgcaacaa gtgtttccaa acgttctgga atagtacgat cccagcggcc aatttcatca
    25861 gggatttttg tgttatctgc aaattcacgc aatgtagaat aaggtgaacc gtctaatgtg
    25921 atcaagcgtt cggatacttc atctaactga gcgttcaaat catccatgaa ctcatccatc
    25981 attgggtgca atgtaaggaa acctggtcct ctcatgtacc aatgtgtttg gtgaacaaca
    26041 acagacattt ggcttagatc tgcaactaat tgatttaata cttctttggt ttgttggaat
    26101 ttcatctttc ttgcctccta acattttctt acacttaaaa taatagtaga ctttcaatga
    26161 aaagtacaac aaaatcattt acctgagaac gattttcagt tcgctttttt atccgtaaag
    26221 ccaatcattt ttcagaatca gctaattctt ctcgcagttg agcaaattgc tgataagtct
    26281 gtgctaaatg cttttgtgtc gaaacaaata atggatagat tttctgatat tttttatgat
    26341 cagtttcttg tactccaact tgctctttca attctatcaa ttcctgtgct tcactgatac
    26401 tttggatctt tccgatgctt ctccagccta aaatcacagc tcctaatgcg gatgcttctg
    26461 tgacagtagg gaaaacaagc gggtaaccca gtacatcagc catcatctga cgaaaaatcg
    26521 gagattgagc aaacccgcct gtagcacgga tctctgtgag ctgattttcc tggcaaactc
    26581 cttttaaaat ccgataaagg ttgagacaga tcccttctag aatcgccttc atcatttctt
    26641 tttctgtatg ttgccgctgt aaaccgataa aactgccaaa cgcatctgcg tcccaaagcg
    26701 gtgctcgctc accaagtaaa tagggatgaa aaatcagacc attggcaccc gctggcacaa
    26761 aagcgatagc cgaaagcagt gcatcatatg gattttctcc tttttcttta gcagattgaa
    26821 cgacttcctg taaaaatgta tgccctgccc aatcaaagat gccagcacca ttgctagtcg
    26881 ctcctcccac tacccaatgt ttctgatcca gaacataaca aaacgtctcc atttctggat
    26941 ggaactgagg ctgagtagta ataaaacgaa gtgctccgct cgttcctacg gtcaaagcgg
    27001 cgatcgtatc accaaaagca cctaacccta gattagaaag cggcccgtct gcccctccca
    27061 aaacaaatga tatatcattt ggcaaaccta gttctgctgc tgtttttgat ggtagacctg
    27121 ttacttgata ggtggaagga aagacttgcg gcaattgctc tattcgaatg ttcaagtagt
    27181 caagcacttc ttctgcccat tggaattcat ggatatcaaa atatccagtt cctgaagctg
    27241 ttccgtagtc gctgatcagt tcgccaaaca atctataaaa gatatagtct ttgataccga
    27301 caaaataagc agtttttttg aataattcag gttggctgtt ttgcagccac ttgattttgt
    27361 tcaatggtgt cattggatga atcggcgtcc cagtctgccg ataaaattct ttgccaagca
    27421 cggtatattt cagtttttct gcttcgtccg ctgctcggtt atctgcccaa gtgatcattc
    27481 gagataacgg tgttttctct tcatccaaca atatcaggcc atgcatcgca gaggagaagg
    27541 agacaaattg aatgattgtg tctgaaggca actgtttaat tacttctttt ataacggtgc
    27601 agacaccttg aaagatcaat tcctgatctt gttctgccat cccttgtgtt tcttggatca
    27661 aagggtagcg aaaataagcg gatgctgctt cttgggcttc atctgtgaaa gcaactgctt
    27721 tggtttgtgt ggttccgata tctaatccga ttgaaacgtt cattttttca cctcaaattt
    27781 tatgtatgta caaaaattta gtttaagctt gcgtatcttg gttttgaaaa gggggagatg
    27841 gcgtgttact ttattttatc attggttgct gtatcggatc atttttatgt ttaaccgccc
    27901 agcggattcc gttaggtcac tctattattt atcctagatc tcattgtgtc aaatgctgtc
    27961 gttcgctttc ttggtatgaa ttgattccga ttctctcgat catcgtccaa cgatttcgct
    28021 gccgctattg caggtgtcgg ttgcccttct actatttgtt ggcagaagcc ttatgtggtg
    28081 gtctttttgc ctggttcttt actttttctt ccgcaagaaa tttttcgacg ttcatatgga
    28141 tgctttcggc tttgctattt tcgttgatgg atttatttta cttcatggtc cattctcaca
    28201 cgctattctg ttcttgggca attttatgga ttttttggtt gcaggccggc gtcttccaat
    28261 ggcaaagtgt gctgttaacg tttatcgtag gcggtgtgtg ccttcgctat ggtcaatcct
    28321 atctcggagc tggcgatact ctgttgatgc tttcttggag tggtggcttg tctctagaag
    28381 agttgcttca gatcttgttt cttgctagtc tgtttggaat atccgttttt tctgtttact
    28441 ttatctgtca taaaaaaaag ttggaaaagc tgcctttcat tccttttttg agcagtgcgt
    28501 tgctgattgt ccttcatctt aggtattgaa aaaaggttgt gacaaacgac ttgtcacaac
    28561 ctctgatata tagtgttctc ttaccaaatc cttgtgactt ggagctaggc gacaggttca
    28621 caacctctcc actaaatggt gttcaatcag ctgaccctca gtattagctc aaaatgctgg
    28681 ttttacaacc tctgatatac ggtgttccat aagtagcttt ttcggcttct tatttcttga
    28741 cgccccaatg tcgacgaagg aaacctgcac gtccagaacc ttctcgataa agattgtaat
    28801 ggagcttgtt tttcttgtaa taatcctgat ggtaatcttc tgcaggataa aattcgcttc
    28861 ttggttcgat ttttgtcacg attggcttat cgaatcgccc gctgtcttgc aatgcttgtt
    28921 ttgaacgctc tgcaatattt cgttcttctt catcattata gtagatcact ggacgataag
    28981 aatcaccgcg atcagcgaac tgtcctaatg cgtcagtcgg atcggtttgc tgccaataga
    29041 tttccaccaa ctgatcataa gaaataactt ctggatcata ttcgatctct actgcttctg
    29101 tatgccctgt tgtgcctgtt gttacttgtt cataagttgg atttggtaca tgcccgccag
    29161 tataaccgga tgtcacagaa atgatccctg gctgctcttc aaatggtttg accatacacc
    29221 aaaagcatcc gcctgcgaaa actgcttttt ttgtcatata aaatccccct tttttctcat
    29281 cataccttga tttccaaaag gaggataacg ttttgcttgt gacagaaaaa acggaaacta
    29341 ttatatacgt gatacggctt ctgataaatg ccaaaacgaa cgtacttgat caaaaacggt
    29401 taaatcagga atcacatatt cagcagaagg aagatttatg ttttcaccta ttcccaatac
    29461 atgcatgtta ccagccaaag ctgcttgaca accagcttct gaatcttcaa tgaccaagca
    29521 tgcatttggc ggtacattca attgctgtgc tcctttgaga aaaacttctg gatcaggttt
    29581 tgcttttgat acttgcgtgc cgtcaataag gacatcaaaa taacttgtca aattcgtttt
    29641 ttctaaaatg aggcgtgcat ttttgcttgc tgatcccaat ccgatcttga tgtttgtttt
    29701 tttcaagtac tcaagaacag ctattgcacc aggcaaaacg gatgtttcat ccattttttg
    29761 aatcgcttga agatatagat tgtttttttg ctcagcaaag gctgatttct ctttttctga
    29821 gaatttctgt tccatttttc caaatgacag caatcgctct agtgactcgg ttctggatat
    29881 tcctttcagt tgctcgtttt ctttctcagt gaatggaatg gacagctggt tagctaaaac
    29941 cagccacgct tcgtaatgat aacgagctgt gtctacaaga acgccatcta aatcgaatag
    30001 tgctgctttg attttcatcg attacctcct tgaaaatgaa cgacttgaag taaagatgcg
    30061 atgtttttag ctgtgcgatg aagattatca gtggcttgct gtaaagcttc ttgtaaaggc
    30121 acagctgcag gaaggatcgg aaaaacggcc gaaatgccaa catctgctgc tttttcgctt
    30181 cctttaccaa cacttccgca aatagctatc acaggaacgc cctctggtac tcttctagca
    30241 atcccaactg gcgcttttcc ttttagactt tgtccatcta gacgtccttc gcctacgatt
    30301 acgaggtctg cttgctgtac ccgtttatcg aactccagta aatccaatac gtagtcgatg
    30361 ccagagacta ttttccctga agcaaatgct aacagtcctg cacccattcc accaccagca
    30421 ctactgcccg atgcctcgat tacttgaggg gcaaatctca tataaaaatc atgcatggct
    30481 tggtcagcca ctgctaattc attttctttc aagccttttt gtggtccgaa aacaaaagaa
    30541 gcgccttctt ttccgcataa aagattagta acatcagtaa tgacagtgat tttggcttta
    30601 atcagctcaa taggcacgtt cgttttatca actgtagcaa ttttacctag attttctccc
    30661 agtgcttcta ctttttgatt atcttgatcg taaaaagcat aacctagacc ttctgccatg
    30721 ccgatgccgc catcgttggt actacttccg ccaacgccga tgatgatgtg tcgaacacct
    30781 tgttgcaaca attgtcgtat cagttctcct actcctcgcg tggataacct aagtggctgt
    30841 tgttggttta gtgggacatc tttcaatccg acgatttctg ccatttcaaa aatcgcagtt
    30901 tgaccgtctg aagcgtactg gatttctttg tttttgccaa atgcattagt aacgactgct
    30961 gtttgccgct tcaaccttaa actgtctgct aatgatgaaa ccgttccttc tcctccatcg
    31021 cctataggta gcagatcata ttcagcctga acaaaaactt cttgaaaacc tgattgaatg
    31081 gcttgtgctg cctctaaggc tgtcagactt tctttgaaag aatctggcgc aatcagaata
    31141 cgcattattt tcccctccgt tccaagatca gttctttttc cagctggtat tggtctccat
    31201 tgacaaaaat aggtaatgtt tctttcgtct ctttttttat tactatttgt ttttttgtca
    31261 aaacaaatag caacctgtga tttttccaaa taaaaggaaa agaaattttt tcccatgctg
    31321 aaggaagtct tggagaaata gaaagagttc cattcgttac tgtaatacct gaaaaaccaa
    31381 atataactgc tagccatagt gctcctaatg aagcagcatg aattccgtca tctgatgaat
    31441 gcggatctgg tcctaaatca atcaggcacg cttcttggaa taaatgatac gcccattcca
    31501 tttcttggat acggcaagcc tctatggcgt gaatcgcttt actcaacgat gagtcgtgga
    31561 ttgttttgtt ttcatagtaa tgcaaattct tagaaacgat tggttgagaa aaatactcag
    31621 gtaataagta taacagcatg acaagatctg cttgcttgct gacttggagt tcatttactt
    31681 cgctacgggc ataatcaagt aaaatagact ggctgccagg acgttctttg taagtggtca
    31741 aatcaataac tggcttactg agatacgtgt catcttgcgg aatcagctgt tctttgttag
    31801 gcactggcaa atataactta tctaaaaaat tttgacattc tttcaaaaaa agcggatcgc
    31861 cagcttgata tttaacagct aaggccacat tataatgcgc cagatagttc gtatatgcat
    31921 tattgtcgat atattctgtg tattcatctg gaccaatgac tctgtgtatc tctaaatgat
    31981 catcgacttt tacagctctg ctcagccaaa aagctgctgt ttcatgaatg agcgtttttc
    32041 cctttgtttc taaaaacgat tgatcctctg ttgcttggac atactcttga acggcataag
    32101 caaggtctgc actgatatga tgttctgcgg cagctgaagc aacttcttgt cttgttccgg
    32161 ttttgatatt caatgcagca aatttgggtg tctcttcctg accagtgtgt gcgctttccc
    32221 atgggaaaag tgcaccttga tagtgataat ctcttgcttt ttgccgtgcg ccttctaatc
    32281 gatcataacg gtacagcagc aaattctttg ctacttcagg aaactgatat aaaaagaaag
    32341 gcaggataaa aatttccgta tcccagaaaa catgcccttt ataaccttcg ccagttagtc
    32401 ccttcgcccc tacactaaca cttggatcat tgcggggagt cataatctgt aaatggtaag
    32461 cagcgaagtc catcgctaac tgatcgaaag aagaatcgcc ttggatcgtt atccgatgct
    32521 gatgccaaaa atctgtccaa taagacatcg actgctctaa aatttctgta taactaacgc
    32581 ttttctgggc ttgggcaata cttttcttaa acgggtctgt ttgctgaaga gaggtagaga
    32641 gcacgatgat cttctccagc gtaaaggacg tattttttct gatcttttct gaaaaggaag
    32701 cggtgatccg gcgatttttt gcttggaata cacctgcttt attgaattta gctgcaatcc
    32761 caatatcaat tttactttct gttgtctggt aggatgcact gatcaaatcg tcaccaaata
    32821 ctcgcagttc tttttcaacc aactgctgtg taccaaaatt ggtttgctgt ccatcgatac
    32881 ccgttttcac atctacttgg cagtcttcgg tcaacggctt gatgaccact ttcagggctg
    32941 cctgatggag attattttga tcagcaacac gacaagtaat gacttccact ttttttcctt
    33001 gagcagtttc aatgagcgca tagcgtttaa gctctcccat ttctaaatct agtgcccgct
    33061 cgtatgcaag aactttcatc gtctcaagag aaaaatgttc accgtcgatt tttatttccc
    33121 atctgatgac atctgggaga ttcactaact cggctgattc tgctcctaaa ggtcggttat
    33181 atacaccagc tgcaaacatt ccccttactt gattccgata gtcttcctcc tggcttcctc
    33241 tgataccaaa aaatccattt ccaatggtca tctgactcgc atacttattg atatactcta
    33301 aatcaaactg tttttcttct actttcataa ggtaataact tctcctgttt ctgccgaacg
    33361 atataaattt tccaccagat tctggatgac atatccttgc cagccatctg caatcatctg
    33421 agtctggtca ccgagcgccc gattcacaaa atgagccata cttagttcat gtgggtcatt
    33481 aactggcgtg gtcttttgta aggaaaccag ctttccatct acatcttcga atagttcgcc
    33541 tggatacaga ttagcacctg cctgatcgcc acaaaaagcc acattcagtt tgtcttctct
    33601 gatattcaaa gcaaacgaag tattcagttg gatgatcttg ccgttttcta attccagtgt
    33661 gccaaaaatt gcatcttcta ctgtgaattt gcttggatcc catttcccga atttccctga
    33721 attttttttc gtaccaattt tttggaagct gtacgcccga acgcttttta ctttaggaaa
    33781 tccagtaata tacatcgctg tatcaagcat gtgaatgcca taatcgatca aaggcccgcc
    33841 tccttgaagt tctttgtcga taaacgttcc ccagcctgga acaccagacc gacgaagcgc
    33901 attcgcttct acgtaataaa tatccccgag aacatcaaga tgattcttga tcattgttgc
    33961 ttcgtcagaa aaacgatgct ggaatccata agccaaaatc tgcccgtttt tatcggctgt
    34021 ctctgccatc tttcttgctt gatcagctgt catagctggt ggtttctcgc acatcactga
    34081 agcattagct tcgagggcag ccaatacatg ttcgaagtga aagcgattgg gtgtacagac
    34141 actcacgata tcgggagaaa tctcattcag catttgtgta actgatgtat acgcatgaga
    34201 aaatcctgta cgtcggcaaa agtcttctgc tcgctggctg actggatcaa ccacagcggc
    34261 caattctaag acatctgatc ttttttgata ataagcagca tgtacttttt ctgctacttg
    34321 tccagcgcct atgattgcta ctttcttcat ttatttctcc ctcctttgct tatttactta
    34381 ttgttattct ttctctattc tttctctaaa tcatttaaat gacgcgctgt ttccagacag
    34441 tttttcaagt attgcaatga ttcctgataa attttcactt cattttctcc tcgcaaacga
    34501 cattcatata ccatataacc ttcatactca ttttgaagta gcgtttgaaa atggcgagta
    34561 aaatcaattg cgccagatcc tggttggtag cgatgattat ctgctaaatg gatatgaccc
    34621 acatactctt tttgcgtagt caatgctttt gaaatatcat cttcttcaat attcatatga
    34681 taaaaatcag cgatgatctg cacatgtttc aattgattag agtcaatgta atgacgcgca
    34741 tccgctaaca aattgatcat atggtcttgg tatcggttca aaggctccaa ataaataacc
    34801 gtacctgttt cttcggctac tttatctaaa taacgaagtg attcgctgac tgctttatag
    34861 tctccttctt tgcttcgggg tgaaaccatc ggcggtaatc gataagtgaa cattccccaa
    34921 gctgctggga taacgattcc tttgcctcca acctcgctta gtgcacgcaa aatctcagca
    34981 atttgacgta caccattaag acgccgttct tcgataaagt caccgatcca cccatcgtag
    35041 ccgttacaag ctgtcactac tgggatccct gtttctttga ttgcctgttt gactgtgtcg
    35101 atattttcta ttaacaactt accgtcgatt tcgtattctt caaatccaca gtctttgata
    35161 aatctaaatt tttctaaaat gtctcttggg aaaaatgctt gatcttgcgt accgaatttc
    35221 attgttctcc tcctcggact tgaacgccca ttttgatact ttcttctggg tgctggtcta
    35281 cataacgcat gtaagcttct gcgctttctg caaatggcac aactggatca atgatttctt
    35341 cacaatccaa gtaaccgttc atcaataatt cccaacatgt gtcctcgatt cgtttacggt
    35401 tccatcttgg gtaatctggg tttggttcac tagatgctcg agcaaagacg atttttgcat
    35461 tattgaaatg tgcttcgcga ccaagattaa acccagctgg aaaaggttta gcaaaggcaa
    35521 cataagaaat gatcccgcca tagccgatcc ctcttaaaga agcttgtaat gcggcagcac
    35581 tgccactcgt ttcaataact gaatctactc cacgtttatc tgtcaattcc tttagacgaa
    35641 aaccgacatc tgctttcata ggatctagcg tgtaatcggc accgtatttt tctgctaatt
    35701 ctctacgctg cgctatggga tcgatcccaa cgatggtagt agcacctgct tttttagcta
    35761 attgaatcgc aatttgcccg atagcgccta aaccgaagat tgctacagca tcacctgctc
    35821 gaacatgtgc ttcccggata ccacctaaag cgaattgagc aggatcgtaa catactgcat
    35881 ttttccattg atccaaccga tcaagtttac gtagtttata attatcaaca ccgtttgtaa
    35941 tcagcgtttc acgaatgggt ccataagtgc agactaactc tcctttttga tactctgtca
    36001 ccaaactgcc cgtttcttcg atcgtaccga caatcatatt tcctaattga aattcgccaa
    36061 ataccacacc gcgttggctt ccttcttctc ttggcaagaa taggcgccat tcttctgaaa
    36121 attcttcatc gataaatgga cttaatcctc taaagtcgac aatttctgtt cctggttttg
    36181 gagtagcgaa aattactcgg atacgtactt cgttcttttc gatctcacgg tcttcgtaat
    36241 ctttgaatcc ggctattctt ggttctaatg caactaattt tttcatcttt tctttcctct
    36301 ttcttttctt tttatccttt tacgccgcct tcgactaaat cacctttaat aaatcgttca
    36361 gataacgcat acataatgat gactggtaaa gcggtcacca atgaagcggc catcattcgt
    36421 ccccaaatgt agtctggtgt actgaataat gtgttcaaac cgattggtaa agtaaatttt
    36481 tcggaggtcg acaggaaaat cgaagcaaat aaataatcat tccacgaaac cataaagcaa
    36541 taaacgaaga cagaaatgat tccagaaata gccaatggta taatgatccg aataatgatc
    36601 tggatccggc taagaccatc gatcattgct gcttcttcaa tatcagtcgg aatcgtgtca
    36661 aaataacttt ttagcatgaa tatcgcagta ggaagtgttt gaacgaccat cgtgataatc
    36721 agtgctgtat tggtatcata caaacctaga ccggaaatga ttttaaataa tggaacaacg
    36781 atcaaaatac cggaaaacat ataaacggtg taaaagctag cattgatttt cgagcgagct
    36841 ttgaagcgta gttttgacaa agcgtatgcc ccgctgatcc caatcactac tgcagttacg
    36901 gaggctatca aggagatcac taaactattt ttgaaataca tgatgaatgg aaaaatctca
    36961 gcattgaaaa tatcctgata atgttcaaaa gtccattctt tcggaaaaat agtttgtgtc
    37021 gttgcaattg cttcttgtga atccttgaat gatgtcatca gcatgatcaa aaatggaaac
    37081 aaattgacag ctaatagcaa caaaatcgtc gcgtaaaata agattttcct gctcttttta
    37141 tttttcattt caagtccacc ttctttcgtg agagcaagac gaccgctgca ataatgatga
    37201 acaatagaat cgatatagct gctgcttttc caagatcatt gaacgcaaag gctgtctgat
    37261 acaaataaac gcctaagata ttgactttat tcgtcagtag atagacatct gtaaacatat
    37321 agaacatcca gatagatcgt aaaacaatca cagttgctag tactggtttg attgccggca
    37381 gtgtcacgat cttgaacttc tgccaagcat ttgcaccgtc catttcagct gcttcataaa
    37441 gcgttttgtc gactgtttgc aaaatagcca aaaatgaaat aaacgcatac ggatagtatc
    37501 tccagatagc aaacaaaacg accataaaga agctagcaac tggctggtcg aaccacagcg
    37561 gtgcttcgtt gaaaagctgc aacctatcta ctaacagatc attgacgatt ccataactat
    37621 tattgaacat atatttccaa gcaaaaatca aagaaataga tggcgtaaca taacttaata
    37681 aaatcagtga acgcaccgtt ttcctaaatc tgaacttgcg gttaagcaaa atagctacgg
    37741 ctaatcctaa agctgtactg cctgctacaa ctaagaatgt gtaagcaact gttaaaccta
    37801 aagaacgata aaattcacta tcacttaaaa tttcaagata attgcttaat ccgataaaaa
    37861 cggcgtctga gttaggattc agcggcatat cgaaaaaact gatctgaata ttggaaagca
    37921 ttggaatcag cactaaaaca gctaataaaa gcatgcttgg tgctaaaagt cccatggcta
    37981 attttaagtc agaatgtttc cttggacgat tcattgaaac ctccccctga taatctgcca
    38041 atagctatct acttttttcg aacaccattg gcagaacttc ttgttatttc gtattttttt
    38101 gtgcgtcagc taaagcttga tcgacatctt cgtttccgac agttacttgg ttcactgctt
    38161 ttccgatcac acctgagctt gtgatatctc ccattttcgt gaaattcttt tccccgacta
    38221 gtccaaagac ttgtacttca tcaaaagcac tagcaatctc agatggcagc tcgccaaaag
    38281 acttgatgac ttcattttct ttgtaaccat ccagttcgac tacttgttta ttcactggtt
    38341 gagcaccacc aggagacatc aagacccact tttccatgtt tttcggttga gataagaaag
    38401 ctgtgaatgc ttcagcagcc tctttttgtt tctcgtctag accagaagaa atcgtcaatc
    38461 cagatactgt gccatagaca gcttgatctt tttgtgttgg gatagcaaaa ccgatatttt
    38521 ctgagttccc cgcttcataa acagaaggca aaatgtaagt tgaatagatt gccattggtg
    38581 ctgttccatt catgaaagca tctttgattt ctgtcacatc attagagcct ggcatcgtgt
    38641 attgggataa gttttgataa aacgacaatg cttctctcat cttttccgta tcgatcgtta
    38701 cctcgccttc atcatccaaa acatttgctc catttgacaa agcaaattga gagaacgctt
    38761 gctcactcat tgttccttca gcagtaggca tagcaattcc atattgttta ttctctttat
    38821 ctgtgaaatg ttgagcaatt tttaaaatat catcccaatt ttctggttct gaaaaaccag
    38881 cttctgataa tttttctttg tcataccaaa taccttgaac ccagccgcta attggtgcag
    38941 caatatagct ttttccatct tctgaacgaa caaggttttt ggctccgtca taataattat
    39001 cttctccagc ttcctcgata acgttttgga ctgccgtctg atcaatcagt tcatctttat
    39061 ccatcacttt agcaaaatcc tgactgactt ccattacttc tggtaattta ccggaacgag
    39121 ctaacgtaac gatttttgta ttgaacgctg attcttctac cggcacttgc ttgattttga
    39181 tgtctggatt ttctttttca aaatcagcaa ccagttcatt gatcacactt aatcgttcct
    39241 gttcgacaga agagtgcatg aattgaatcg ttgtctgttg tgattcgtta gagctttgac
    39301 tttcaccaca agcagtaaga agaaatccag tagctagcag acaggtagta acaataattt
    39361 ttttcatttt tttccacctc ttattttatt attttcagcc acatatattc atacggctgg
    39421 aatatcaact ttcgttcttg actgcaatgg ataatttgtc cagaaaacag gtctaggtaa
    39481 tctccttttg gtaaaagata acctgctgat tgattggaaa aattatggat aactaagact
    39541 ttctcttcct tcgcttcgcg aataaaactg ataaaagaat ctgctgagtc cagtaagtaa
    39601 atcaaactat tcggatggaa ggctttttct ttttttcgaa tagatagcat ctgggtcaat
    39661 tcaccatata ctgctgttct taatggatct gtctgcaact ctttctcaat atctgctaac
    39721 aaaaattttt gccgattgat ttttcgattc tctcctgttt tttctacgcc tttgtaatca
    39781 tttcgacttc ccagcacact ttgaatataa attgccggca cgcctggtaa cgttaccaaa
    39841 aatgaatgag cagctaaaaa gcgtttgctt ctccattccg tcgtatctgt tggagaactt
    39901 aacgcatcta gatacgtagc gtttatctca tatggactgg tcgttccatc tgggtttttc
    39961 ttgtagttta ctcgggtacc ctctttcttc agctgttcca ctaattcaag gatcgttttt
    40021 tccggcagaa tacctcgtac cggattcaat ccgataccgt catgggaggc taaaaaatta
    40081 aaaaaagttg tttttttcga tgcgaaatcg atggttttta accattcttt caacttactt
    40141 gtattacccg aacgaatcgc ttgaagggtc aaaggtggta gcgaaaactg atagaccatc
    40201 tgtgcttcat tctcgccatt cccaaaataa ctgatattat ctttgtgtgg cacatttgtc
    40261 tcagtgatca ataccgttcc tggagctgct tcatcagcga ttgcacggaa taattggatg
    40321 attttatgcg tttctggcag gtgtatacaa gaagttccta gttctttcca taaaaatccc
    40381 acagcgtcca agcgaataaa tgaagcgcct ttttctaagt aaaacaaaag aatatcgatc
    40441 attttcaata aaacttctgg atttttaaaa ttcaagtcta cttgatcttc gctaaaagtc
    40501 gtccatacgt gtttttcttt cccgtttttg tcaatgaacg ttgtaagtaa cgggctggtc
    40561 cttggtctag tcacgctgct tgtatctgta tccagtggca aatcaataaa aaaatctttg
    40621 tacgtatcat tcaagtttaa atattcctga aaccaagtac tttcggcaga catatgattg
    40681 cagacaaagt cgaacattaa ccgtgtttcg cttctcattt cttctatatc ttcccatgtg
    40741 cccaaacggt cgttgattgc ctgataattt acaacggaga aaccatcatc agatgtatat
    40801 gggaaaaatg gcaagaaatg tacaactcta aatgtatctg ccaaatactt ttgataaaat
    40861 ttcttaaatg tacgcaaagt tggttgctgc tcttcaaaaa attgatcccc ataagtaatc
    40921 aagaaaatat cttcttcgtc ccaatgcagt ttgcctgcct ggcctttctt ctgctgataa
    40981 acactgactt tttgttcaat catagaggcg gttttctcag acaaatctga gtcgcgataa
    41041 atcgcttgta aaagttttgt atacatcgga ttcccctttc ttttttggga gcgttcccaa
    41101 cgatttgatc atattatgga aaacgcttac tgtcaatagc atttttttaa ataattttga
    41161 tatacacaaa aatacaaata agaaagctta tgtatcaata attaatagct tcttaaaaaa
    41221 atatggtctc tcatgatcat gcaagctatt cctaaatttt caaaatcata gacaaaaaag
    41281 aatgtctatt gtactatgga attattctaa aacgctccca aaaaggaggt gcatataaaa
    41341 ttgaaaccta ctatttatga cattgcgaaa aaagctaatg tctcaaaatc aactgtctcc
    41401 agagtattaa ataaccagcc aaatatctct gataaagcaa aagcaagtgt acttcaagca
    41461 atcaaagaat tgaattatga accgagcaaa attgcaagag gactgtcagg aaatggtttt
    41521 gacgcgattc tagcattatc tcatcgtact tctcatacga cctatggcaa tccttttttt
    41581 tcagatgtta ttcaatccat ttctgccgtt tctgaacaaa ataacttcga cttgattcta
    41641 caaacggcaa aaacaccaga agaagagcta gaaaaatgcg cgaaaaagat ccaagaaaaa
    41701 atgatccgag gaattattat tttaagctct acgatggatg aaacacttct ttttgaattg
    41761 gatcaataca atattcctat tgtggtaatc ggaaaagtcg gaaacgacta cacgaacatc
    41821 tactcagtgg acacggataa tttccaggat agttacgatt tggtggaaac actgatcaag
    41881 aatggacacc gtcatatcgg ctgtctccat gctccgacag aagtttatgt atccaatgat
    41941 cgtctcaatg gttaccgtca atgcttattt gatcaccacc tagatatccg taatgaatgg
    42001 attatagacg gcggctttcg cttagaagat agcatggcag caatgaatga tttaatgaag
    42061 ctacgagaaa gaccaactgc gattttttct accgatgcat taaaatcatt gagtatttac
    42121 aaagtggcag aacaatctgg aaagcagata cctgatgatc tttcgatcgc aggattcagt
    42181 gatcccttat tgtcctccct attttcgcca cagttgactc ggatagacat tccgacaaat
    42241 cagttaggaa tcaaagccac agaactttta ttccaactaa tccaccacca ataccctgaa
    42301 gaaaaaagaa tcattctttc aaccgagatt actctcacag attcgatcca acaacaagaa
    42361 atgtagaaca gaattataaa agaacagaaa cactgaggat aagtcagtta ctctgttctt
    42421 ttttcaatca ctcatccctc gttaccccct cccggtattt tagcgaattt ttcttatttt
    42481 ccatctgttt tagatagctg tggataacta taatgtttgt gttaaactat tttttccaca
    42541 gggaagaaaa aactattttt aaccacgttg tgtataagtt tttatagttc gtttttttaa
    42601 acacatttac ccttatttca atccatgtta tttggggata cttatctcta aaacataaat
    42661 agaataacag acatgtctac tggttttgtt ttattcgagg aattattcaa taatagctag
    42721 caaaatatta aaaataaccg ctctatcttc ccatcaattt attttgcatt caaaaaatga
    42781 tctatcttta cttttgttga ttgacttctc ttggaagatg atcgttccga taccttaatt
    42841 ggatacgaac cgatgttgtt aagatccttt cccacttttc ttctagttag acaaattatt
    42901 ttagac
//
LOCUS       DAZBTF010000016        41787 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000016 DAZBTF010000000
VERSION     DAZBTF010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.016"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..390
                     /locus_tag="ACHGNS_000958"
     CDS             <1..390
                     /locus_tag="ACHGNS_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFN6936921.1"
                     /translation="LFSLFLSGTALLSYNLYLSVQIKGLIFLAIDFILLFALLCLFAT
                     YQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSGIILGVTWQFKGLILFGV
                     IGLLTVWNGTMTTHWREELDKQLESYE"
     gene            383..2107
                     /locus_tag="ACHGNS_000959"
     CDS             383..2107
                     /locus_tag="ACHGNS_000959"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN6936922.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            2104..3555
                     /locus_tag="ACHGNS_000960"
     CDS             2104..3555
                     /locus_tag="ACHGNS_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFN6936923.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(4028..6181)
                     /locus_tag="ACHGNS_000961"
     CDS             complement(4028..6181)
                     /locus_tag="ACHGNS_000961"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFN6936924.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(6183..7229)
                     /locus_tag="ACHGNS_000962"
     CDS             complement(6183..7229)
                     /locus_tag="ACHGNS_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN6936925.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            7379..8668
                     /locus_tag="ACHGNS_000963"
     CDS             7379..8668
                     /locus_tag="ACHGNS_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFN6936926.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            8681..11383
                     /locus_tag="ACHGNS_000964"
     CDS             8681..11383
                     /locus_tag="ACHGNS_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFN6936927.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            11368..12225
                     /locus_tag="ACHGNS_000965"
     CDS             11368..12225
                     /locus_tag="ACHGNS_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFN6936928.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(12296..13153)
                     /locus_tag="ACHGNS_000966"
     CDS             complement(12296..13153)
                     /locus_tag="ACHGNS_000966"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFN6936929.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            13364..15535
                     /locus_tag="ACHGNS_000967"
     CDS             13364..15535
                     /locus_tag="ACHGNS_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFN6936930.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            15603..15944
                     /locus_tag="ACHGNS_000968"
     CDS             15603..15944
                     /locus_tag="ACHGNS_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFN6936931.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(16220..17974)
                     /locus_tag="ACHGNS_000969"
     CDS             complement(16220..17974)
                     /locus_tag="ACHGNS_000969"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6936932.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(17985..18821)
                     /locus_tag="ACHGNS_000970"
     CDS             complement(17985..18821)
                     /locus_tag="ACHGNS_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFN6936933.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(18823..19506)
                     /locus_tag="ACHGNS_000971"
     CDS             complement(18823..19506)
                     /locus_tag="ACHGNS_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFN6936934.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            complement(19665..21059)
                     /gene="uxaC"
                     /locus_tag="ACHGNS_000972"
     CDS             complement(19665..21059)
                     /gene="uxaC"
                     /locus_tag="ACHGNS_000972"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFN6936935.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            complement(21098..22597)
                     /locus_tag="ACHGNS_000973"
     CDS             complement(21098..22597)
                     /locus_tag="ACHGNS_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFN6936936.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            complement(22590..24011)
                     /locus_tag="ACHGNS_000974"
     CDS             complement(22590..24011)
                     /locus_tag="ACHGNS_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFN6936937.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            complement(24071..25063)
                     /locus_tag="ACHGNS_000975"
     CDS             complement(24071..25063)
                     /locus_tag="ACHGNS_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN6936938.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            25295..27493
                     /locus_tag="ACHGNS_000976"
     CDS             25295..27493
                     /locus_tag="ACHGNS_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6936939.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            complement(27951..28823)
                     /locus_tag="ACHGNS_000977"
     CDS             complement(27951..28823)
                     /locus_tag="ACHGNS_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6936940.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            29123..30097
                     /locus_tag="ACHGNS_000978"
     CDS             29123..30097
                     /locus_tag="ACHGNS_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6936941.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            30119..30811
                     /locus_tag="ACHGNS_000979"
     CDS             30119..30811
                     /locus_tag="ACHGNS_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFN6936942.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            30777..32285
                     /locus_tag="ACHGNS_000980"
     CDS             30777..32285
                     /locus_tag="ACHGNS_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFN6936943.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            32359..33462
                     /locus_tag="ACHGNS_000981"
     CDS             32359..33462
                     /locus_tag="ACHGNS_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFN6936944.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            33465..34802
                     /locus_tag="ACHGNS_000982"
     CDS             33465..34802
                     /locus_tag="ACHGNS_000982"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFN6936945.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            34789..35667
                     /locus_tag="ACHGNS_000983"
     CDS             34789..35667
                     /locus_tag="ACHGNS_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFN6936946.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            35664..36587
                     /locus_tag="ACHGNS_000984"
     CDS             35664..36587
                     /locus_tag="ACHGNS_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFN6936947.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            36584..36994
                     /locus_tag="ACHGNS_000985"
     CDS             36584..36994
                     /locus_tag="ACHGNS_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFN6936948.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            36998..38566
                     /locus_tag="ACHGNS_000986"
     CDS             36998..38566
                     /locus_tag="ACHGNS_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFN6936949.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            38806..40224
                     /locus_tag="ACHGNS_000987"
     CDS             38806..40224
                     /locus_tag="ACHGNS_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN6936950.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            40236..41546
                     /locus_tag="ACHGNS_000988"
     CDS             40236..41546
                     /locus_tag="ACHGNS_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFN6936951.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
BASE COUNT        13564 a         7375 c         8552 g        12296 t
ORIGIN      
        1 ctatttagtt tatttttatc tggtactgct ttattgagtt acaatttata tttatctgtt
       61 caaatcaaag ggctcatttt tttagctatt gattttattt tactttttgc cttgctctgc
      121 ctatttgcta cttatcaata cagtatgatt ttagatagtg aatatacgat ctctgttcct
      181 aatctgctaa agctaagttt tatttcggtt ttttccagct tttcgtcttt tctaaaaata
      241 attattggta gcggtataat tttaggcgtg acttggcaat tcaaaggatt gatcctgttt
      301 ggtgtcattg gtcttttgac ggtttggaat ggtacgatga caacacattg gcgtgaagaa
      361 ttagataaac agttggaaag ttatgaataa gtttcggatt ctattttcaa aaggaacctt
      421 actaaatcgt ctgttgcgga aatacgcggt tatcatgatc ggtgtaacga tgactgctac
      481 cgttattttt agtatccata cttgggaaca aaatcaaaaa caggcagaaa acatgacaag
      541 tgatgccgtt caaagcacaa gcagaatgct gaatgataaa acaaccttga gtaggatcat
      601 caaaaaccag cttgtaggga attccgaaaa gatcgaaaat gtgacgacct acttaacaaa
      661 acccatagat cagtatctga tgtatgtata cgaacagcaa aacagtaccg atgaattagt
      721 ttctttccca aatcaaatca aagatctcta cgcaaattat gaagaactaa gtgcgatata
      781 tatcgtgttg aatcaactac cggagtatta tgaatcttct agagaaaaca aaggtgggga
      841 aataaaaaag gggacaccaa aattactaga cgccttttat atcaagttgc ctatcacaca
      901 aggaggagct gagtcaggaa gtatttttat tggttttcaa aagaaagaac tagatatgat
      961 tttagaaaat ctaacgtcat tcaatggact ctctttatac atgatttctg gaacgacaaa
     1021 tcgactatac acattccatg ataataaaat agctaaagaa acgtttgaaa gacaagaaat
     1081 gatcatcaca gagagtctga agaaaaattc acaattgccg attgacgatc tggaaaaaaa
     1141 caattttgtt caacaccaag aactgtctga tgattatcgt atattagctg tactagataa
     1201 aaaaagtgta aaaatcgaaa cgggaaaaaa tcttgccccg ctgatcattg gtatcattat
     1261 tttagacaat atactgctta tctttcttta taaaacattc aaacgatatt cacagcaagt
     1321 atctatcgtc atgcaggcca tggatcaaac agcagcaggg aatttagaga cgcggatcga
     1381 cataacaaac acggaatatg aactgaagga actttcaatc ggtatcaacg aaatgttgga
     1441 tagcatcaat cagtttgtag aagacatcta caaattagaa atcaaacagc aagacgcaca
     1501 tatgcgggcg cttcaagcac agatcaatcc acacttttta tataatacat tagaatatat
     1561 ccgaatgtat gcaatcagcg aaggaagtga agaattagca gatgttgtgt atgcgttttc
     1621 cgctttgctt cgaaacaata cgaatcaaga gaaaacgatc actttaaaag aagaactgga
     1681 cttttgcgaa aaatatgtct atttgtatca aatgcgttat cctaatcgag tagcctatca
     1741 ttttatgatc gatcctgatt tggaaaagat cgaagtacct aaatttgtga tccagccatt
     1801 agttgaaaat tattttaaac acggtattga ttttactcga ttcgataatg cgctaagtgt
     1861 caaagtcttg caggaaggca aacgggtaag gatcatcatc aaagataatg gaaaagggat
     1921 gactgaaaaa cggctaaaac agattgaaga aaaactgtct catcctaaag tggaacttca
     1981 cggttctatc ggtctgcaaa acgtgaatga acgtttacgt gccagcttcg gcagcagtta
     2041 ttatatgtcg cttgagaata atgagacagg tggactgacc gtttctatta cattcaagga
     2101 gggatgagcg tgtatcaagt gttattggta gatgatgaat acatgatttt gaatgggtta
     2161 aaaaagataa ttgattggca aagtttaggc tttcaaatag tagcaacagc agagaatgcc
     2221 aaagaaggac tagctgtact tgaacagcga cagattgatt tagtagtaac ggatgtgaca
     2281 atgccagaaa taaatggact ggaatttatt gaagcggctc aaaaagaacg tcataatttt
     2341 gaatttatga ttttatcagg ataccaagaa ttcgattatt tgaaaggagg tatgcaatta
     2401 ggcgccgtga attatttgat gaaacccgtg aataagttcg aattggttga aagcctcaag
     2461 aaaatcaaaa cacggttaga tcagcaaaat gaacaaaaaa atcaacaaga aatctatcaa
     2521 gaactgttat tcagtcaatg gttgaatgaa gaattggatg aaataagtga agaagaaata
     2581 ataggacaaa taagtgagaa gcagcatagg atagtattga ttcaactggc aagaactcat
     2641 gatactctta tcaatagatg gttgcaagaa cggcaacaaa tcttttacta tcaacgaaat
     2701 tacggagacc gtgtattgat cacgttgttg ttggaggcag atgaagttga tggcttctgt
     2761 tgttttgttc aagaaaactt tcctgatcag gaatggctga tcagtatcgg ggaagagaca
     2821 gattctttgg acaaaatccc tgaaacattc caacaagcga aagacaatct acagttgcat
     2881 caattttatg gagataaaga cgagcatgtt ttttacgcag tacaagcaga aacaaaagat
     2941 caaaccatcg atttttcaag tttcagacgc gtgctgcaaa ataaacaatt ggaagtcgct
     3001 caaaaaatga tcactgattt ttttgaacag tttcagttag cagccatgat gccggaagat
     3061 atacgatatt ccagtttttt actgtttatg gagattcaac gggaattgat tgatctggaa
     3121 gatgaggaat atttacaagg aatcgaaaaa atccatcagg caaaaacggt gcaagaactt
     3181 cagcagctac ttctgtcatt catcaaaaag catcagagac agaaaaagta cagtgacaat
     3241 gtggaaaaag taatcgtaat cctgcaccag cattatcaag aaccacttac actcaaagaa
     3301 gtgtcagaaa acctgcattt aaatgtcatg tatctaggac agctgttcaa aaaagaaacg
     3361 aaaaaaagtt tttcagctta tttgaaccat ttacgtatgg aaaaagcgaa acaactgctt
     3421 ttgcacagta atcaaaacat caatgagata gcaagtgaga ttggctacaa caacaccacg
     3481 tatttctcta aattattcaa gaaaatcgtc ggcagatccc ctaaagagta ccgagaaaac
     3541 agtggagaat cataaaaaaa aaagccaatg taatgaaata acttataaaa aattgtctag
     3601 aaatctaatt ttgggctgtt cttattactt gaggcaaggc agctttttca tgacctctcg
     3661 ataaacggag ttcaaaagcc agtttctcgg tattaagcca aatttatgca aaaatgtgga
     3721 aagccatttt cacaaaattt ttcttaatac tcgaaactaa acagcttttt cacgacctct
     3781 ccactaaatg gtgttcaatc agctgaccct cggtattagc tcaaaaaatg gcaaaatatg
     3841 aggaactatt ttgccatttg tctctctaat actcaggtca gaacgctgat ttcacaacct
     3901 ctcaataaac ggtgttcaaa agcttgcatc tgcggtaata agaccaacca aacgaaaaat
     3961 atgaggatct atttttgctt ggctgttctt attacttgat gcaggacagc tttttcacaa
     4021 cctcttgtta attatagttt gataaggaaa aaggcaaatc ttttgcttga tatttcttag
     4081 ggtgagggat gatccctaat tggtaagaga tcgtgccgcc tttcagcaaa tcttgatggg
     4141 taaaatataa tttcgtatat tctctgtcca tccgtttgat tttatggata aattgttgct
     4201 gcggccgatt aggcgttgct tcaatcgtca attcctttcc atttgacaaa agcaaaacag
     4261 ccttgtcaat taatggcata ccgataacat attcaccggt tccaggcgtg acaggataaa
     4321 atcccagact attgaagata taccaagcgg ccatacttcc attgtcctca tctccagggt
     4381 aacccattgg agaatcgtcg aaggtctgag tcatcaactg tttaagcagt ggctgtgcca
     4441 tttcaggttg tcctatataa ctgaacaagt aaggatagtg aaaacttggc tggtttgaaa
     4501 tagctaattg tccaaattct atcgcggcca tctcactcat ttcgtggatc tcaaaaccat
     4561 atcctcctgg gtcaaacgtt gggggctgat tgcacagctc aatgagtttt tctttatatc
     4621 tctcactgcc accatatttt ccgatcagtc ctgaaaaatc gtgataaaca gcaaagctgc
     4681 tttgccaagc acttccttct gcataatctt gtccccaacg attagctgaa aatggttctc
     4741 tgaacttgcc atctttatct tttgaacgca taaatcctga ctcatagtca aagatatttt
     4801 gatagttcaa tgattgctgc tgatatttct tcgtcatttc ctcttcttct agtaccttgg
     4861 caacttggct aatacaaaaa tcgctataag catagtctaa tgtgtgattg actgattcgt
     4921 ggtagtgatt aggaacatag ccatatgtca agtaatcttc tgttccttgg cgtccataat
     4981 ttggatgttt gctttgaacc gtagcacctt tttccattgc cgttagaaat tcaggcatca
     5041 agtctcgtcg gatatctttt acagcagcat ctgcaatgac cgcatcgatc aatgtcccag
     5101 gcataagtcc acgttcatca ggagacagcc attttggcaa atagccactt tctctatagc
     5161 tgttcaaaaa accttccagc atttcttcgt atttttctac agcaatcaaa gaataaagag
     5221 gataaaccgt tttatacgta tcccaaaacc cattatttgt atagagaaca ccaggtttta
     5281 tagatcgact agtcgtatca tagtggattt tttgcccact aggatcaatt tcataaaatg
     5341 tttgtggaaa taaaaacacc cgatataaat tatgataaaa cgtccgcaat tgttgttgat
     5401 catggtgtgt gagctgtata cgatcaaaat aggactgcca ttgttcttga ctgtgagcaa
     5461 ggtaagtctc tttgctttgg ttttgttcta gttccaggtt ataagctgct tgttccgagc
     5521 tgataaaaga tgtcccaaat cggatagttt gtttttttgt ctgttcaaaa tacaacggga
     5581 tcgagtcgct atttttgttg gaaacagcta actcatcaat tgttaaagga tattcaaatc
     5641 tcaatataaa ataaaaagaa aaatcaggat cttctgaacc agcaaaatgt gtgatttttc
     5701 cttccactgt gtaaggatct ataacaaaga gctgatggcg tcccggaaag gataaaagta
     5761 aaccgctgtc atttttgctg taatcaatag ataagattcc gccatacatg ctaggtatca
     5821 atgtagaaca gatgccataa cgtaattgac agatcgacag atgagtcggg caaaatatac
     5881 tttcttctgg gcggtaagaa ctttgagcat ggaaaagggt gttctcctgt agttttccat
     5941 tgatcggcgt aaacagcata tgactgaaat cacccatcca aggacttggt tgatgggtaa
     6001 gacggtagcc ttgaaaaacg cggtcatcag gatgaaacca ccagctgccg ttttgatccg
     6061 tagtttgcgg tgcaaaataa ttcatcccaa aaggaacgcc agtataaggc tggcagttcc
     6121 cattagagaa atttggctga ttcgctgttg catgtctagt atctatttga ccgatattca
     6181 ttttatttct cctttgttga ttgtcttttg atgaatgaaa ctggaaattt actatcggat
     6241 ggtcgatctt ttgtctctaa ccaagtgacc aaagcatctc ccgcaagctt acctaactgt
     6301 tcaaaatctt gtgcgatggt tgtcagtggc gggtcaacaa gagctgctgc ttggatatca
     6361 tcaaatccga taattgaaaa atcttcaggg atatgataac catgctgtct caagagattc
     6421 atggtctgga tagcgacaac atcgttttca caaacaaaag cagttacttc atacttagtg
     6481 atataagaaa gcaattcatc taatgtcgcg cttttgtcat ccagtgaagt gtggaaagtc
     6541 aaaccagctt tgtccaatgc ttcaaggtaa ccaaggtaac gctgtcgaac ggattgtgga
     6601 tgagtctgcg taccaaacaa ataagcgatt tttgaatgtc cctgttcgat caaataagag
     6661 gcagcaagcg caccgccttg gaaattatcg gaaagaacgg taggaaaagg taactcatag
     6721 atttttttat ctaatatgac gacagggaat tctttcaatg aaagctcaaa aagtaagtca
     6781 agatgttgtt ctgtatcaag agcataataa atcaatccgt caaattcatt gattagctct
     6841 tttgctgttt tattttgtag aaagtcaagc gtcgtcatca tgacatcaat ctgtttatct
     6901 tgcatcacag ggttcaatcc cttgctaaaa tcaccaacag acaaatcagc gacatatgga
     6961 agaagaaaca gaatccgtcg tgtttttgta ggagaagcaa gaatctgttc cggagaagcg
     7021 gatttaacga aactgccttt cccgcgaacg cgataaataa gtcctaattg ctctaattca
     7081 gtcaatgcac gtttggaagt gattctgctt actttatagg tacgtgaaag ttctttttca
     7141 gttggtagtt ggctgtctac aggtaattca ccagttaaaa tagccgtttg caaatcatta
     7201 aatattgtct ggtacaatgg ttgagtcata taaacacctc ttaatgatat atcatattca
     7261 aataatacca taatggatga aaaagtaaat aaaaagatat tttttgaaag cgtagacata
     7321 attggctgta tgaagtataa tctttaaaga tatatcaaat agaaagaagg ttttattagt
     7381 ggtttataaa gatataccaa aatcggtgca gaaatttatg gaagatatca cacaaaaatg
     7441 tggggatatc catgcggact gggcaaagaa ttttacagca gcatttgcca atactctgtt
     7501 gacaacagtc aaaaagcaaa atgacggaac caccttttta ttgacgggag acattccagc
     7561 gatgtggtta agagactcta ctgcacaagt tcgtccttat ttagtgattg caaaagaaga
     7621 tgaggaatta gctgacatga tcagcgggtt agtcaaaaag caattttttt atatcaatat
     7681 cgatccatat gcgaatgcct tcaacgaaga agctaacggt gctggccacc aaacagatca
     7741 tacaaaaatg aacgattgga tctgggaaag aaaatatgaa attgattctt tatgctaccc
     7801 aatacagttg gcctatcttc tttacaaaaa tacaggaaaa actgatcaat tcaatcaatc
     7861 ttttattgaa ggtatcaaaa aaatcttgga cgtgttcaaa acagagcaag atcatgaaca
     7921 ctctccttat acatttgtac gagacacgac aagaaaagaa gatacgctga tcaatgacgg
     7981 gcgaggtgca aaaacagctt ttacaggaat gacatggtct ggtttccgtc ctagcgatga
     8041 tgcttgccgt tatggatatc tggtaccatc caatatgttt gcggtggtcg tgttaggtta
     8101 tgtggaagaa atttttcaaa cgatactcga tgataaagaa attgtacagc aaacacagaa
     8161 attgaagaaa gagatccaag aggggattca gaatcacgga cgaacggtta atcaaaaagg
     8221 gcaaacgatt tatgcatatg aagtagatgg aatgggaaac gccacagtca tggatgacag
     8281 taatgtgccc aatctgatcg ccgcacctta cttgggatat tgttctgaac aagaggaaga
     8341 ttatctgcaa acaagaagaa cattgttgag taaagaaaat ccatatttct atgaaggaaa
     8401 gtacgcaaaa ggaattggat cctcccatac gccagaaaaa tacgtttggc cgattgcatt
     8461 agctatggaa ggaatgacaa cgtcagacaa atctgaaaaa gagcggatat tgaatttact
     8521 tgtagcaact gacgcaggaa cacaccttat gcacgaagga ttcgatgtga acaatcctga
     8581 aaattatacg agagaatggt tttcttgggc aaatatgatg ttttgcgaat tagtcatgga
     8641 ttactttgat attagagtag aaaagtagga ggtatcgtaa atgaaaaaga aagtatatat
     8701 catttctcat agccactggg atcgtgaatg gtacatggct tacgaacagc atcacatgcg
     8761 actgattgaa ttgatagacg atctactaga gctatttgaa gttgatccgt ccttcaacag
     8821 ttttcatttg gatgggcaaa cgattatttt agatgactat cttcaagtaa ggccagaaaa
     8881 aagacaggca atccaacaag caatcaatga aggaaaattg agaattggac cattttatat
     8941 cttgcaagat gattttctta tcagctcgga atcgaacgtg agaaatatgc tgattggcat
     9001 ggaagaaagc cgtaaatggg gaacccctgt catgttaggc tattttcccg atacatttgg
     9061 aaatatggga cagacgcctc aattaatgaa acaagctggt atttcagcag cagcgtttgg
     9121 ccgtggtgta aaaccaattg gctttgataa tcaagttttg gaagcagaaa actattcttc
     9181 ccaatactca gagatgtggt ggaaaggccc tgatcaaaca gcaatctttg gtctgctatt
     9241 tgccaattgg tatagcaacg ggaatgagat tccagtagaa aaagaagcag ctcttgcctt
     9301 ttggaagcaa aaattagcgg atgcagaaca atatgcctct acgaaccact tattgatgat
     9361 gaatggtgtt gatcatcaac cggtacaaaa agatatatca aaagcaatcc atctcgcaaa
     9421 tgaactgttt ccagattacg agtttattca ttctaatttt actgactatc tggaagcagt
     9481 tcaaaaagat gtgccagaag acttaggctc agtagaagga gaattgacaa gtcaagaaac
     9541 ggatggctgg tatactttag ccaatacagc atctgctcgg gtttacttaa aacaatggaa
     9601 taccaaggtc cagcgacagc tagaaaatat tacagaacca cttgcgacga tggcttatga
     9661 agttagtgga aactatcccc atgatcaatt agattatgct tggaagacat taatgcaaaa
     9721 tcatcctcac gacagtattt gcggttgttc agtcgattct gtccatcggg aaatgatacc
     9781 tcgttttgaa aaagcagatg aagttggaaa gtatcttgca caagattcct tagaacagct
     9841 gaccgcagca atcgatacga ccggattccc aaaagacagc tttccatttg tcattgtaaa
     9901 tactgcagga atggataaaa ctggtgaagc agaaataacc atcgaattag aaagaaaaag
     9961 attcgctgaa ggaatacctg aacaactata tcaagagtta gaaaatttgc ctaaacgtaa
    10021 gtatcatgta gaaacaaaaa gtggggccac tataccagct attttatctg aagaaactgt
    10081 ccaatttggt tatgatttgc cgaaagaccg tttccgtgtg ccatatatgg caagaatgat
    10141 caaagtgact ttaccattag aaaacatgcc agcattttct tgggaaacat tcgcgcttgt
    10201 agaaggggaa gcgaaagcag aagaaaagga aacaatgatt catcagagtg gacgaatcat
    10261 cgaaaacggc cctctacatt tgacgattga aaaaaatggt accattacaa tggaagatcg
    10321 caaaaacaaa cgaaagttga ataatctgca tatttttgaa gatatcggcg atataggaaa
    10381 tgagtatatc tttaaacaac ctttctgtga tcagccaatc ctatccagta acaaagaaaa
    10441 ctctgaagta aaagtattag tagatacacc ggaaatagca aaaataagta ttctacagga
    10501 aatggaaatc cctgtttctg cagacgagcg tttagaaaaa gaacagcaga tggttgtgga
    10561 atttcgttat cgaaaagcag aacgatccaa agaaaaacga atccttcaaa taaaaacaat
    10621 catgacaatc agaaaagata gtaaaaaaat cgactttgaa acgactctcg acaatcagat
    10681 gaaagatcat cgcttacgtg tcttgtttcc tacaaagtta catgtggaac atcacgaagc
    10741 agatagtatt tttgaagtgg tgaagcgccc aaaccatgtg tctaaaagtt gggaaaaccc
    10801 cacgaatccg caacatcagc aagcatttgt caatattcat gatgaagagt acggtgtaac
    10861 agtcggaaac tttggtttga atgaatatga agtaacagaa gacggacaaa tcgcagtgac
    10921 gctacttcgc agtgtgggag aactaggcga ttggggatat tttccaacac cagaagcaca
    10981 gtgtctaggt gagcatcggt tcaattacag cattgaacta catggaccag aagaaaaatt
    11041 ctctacttat ttgcatgcat acgcagcaca gattcctttt tccactcaac aaatcaaaca
    11101 tcatgaagga acattgattt caaaacaaca atatttaacg ataaaaagtg aaacgtttgc
    11161 aattacagct ttgaaacgca gcaagtttag tgataaagta gtagtaagag gtttcaatat
    11221 gtctagtcat ttagaaaaat tggaaataac taaagacaat ggaaaaactg tcatattgaa
    11281 tctgcttgaa gaaccaacga agcaagcggt tgtcccaata atccaacctt atgagattcg
    11341 gacaatcgga ttcgaggagg aaaacgaatg tatggcggaa tagaagcagg cggaacaaaa
    11401 tttgtgtgtg cggtttcaga tggtggagaa attttagaaa aattaagtat ccctaccaca
    11461 acaccagaag aaacattgga tcaggtattt gaattttttg atcgttatga attgaaggga
    11521 atcgggattg gctcattcgg cccgattggt atcgacaaaa caaaagataa ttacggatac
    11581 gttttggcaa ctcccaaaaa agggtgggca caattcgatt ttcttggcac aatcaaggaa
    11641 cgctacaata ttcctgctgc ttggacaacg gatgtcaatg ctgctgcata tggggaattg
    11701 aagcaaggag cagcacaagg aaaaaatagt tgtatttacc ttaccgtggg aacaggaatc
    11761 ggtgcaggtg tcgtgatcaa tgaagaaatt ttttcaggga tcgcacatcc tgaaatggga
    11821 catatttggg tgaaacgcca tccagatgac cattatgaag gtacttgtcc ttatcataaa
    11881 gattgtttag aaggattagc agcaggaccg tctattgagg cacgcacagg gatcaaaggg
    11941 caagatctgc cgcaagatca tcctgtttgg gctattcagg cttattatat tgctcaagca
    12001 ctgatcaact atacgttgac gttagcacct gaaaagatta ttttaggcgg tggggtaatg
    12061 aatcaggatc atctccttca aaaaatccgt cagcaatttg ttgaattgat gggtggttat
    12121 atggaaacac ctcaagtaag tgaatatatc gttcgttggg gactgccgaa tgaaagtggg
    12181 attatcggta gtttgctgtt agcagaacac gaacatcaaa agtaagcaga caaattacac
    12241 aaaaagaaca gagtcttatc attatctgct gataagactc tgttcttttt tatttctatt
    12301 cccataatcc aggtaaagct ttaccgacta ttttttcttg agtaaatgga tggatcatgt
    12361 gcagttctaa tgcatggagc atcagtcgtt cagctgttct attttggtag agagggtctc
    12421 caacgatcgg atgtccctta tgagctaaat gaactctgat ctggtgtgta cgtccggttt
    12481 ccaaaacaca atagacttgt gtttgttcag aaagtacttt gttgacagtg acatgtgtca
    12541 aggcgctctg accattttta tcatctacga ccctttttct ccgatcatgg cgatctcgtc
    12601 caattttatt ggtgatggtc atatctttgg caatttctcc ccatacgatg gcttgatatc
    12661 tccgatagat cattttttgt tccaacattc ttccaagtat cggcaagaca aatggatttt
    12721 ttgcaaataa aacggcaccg cttgtttctt tgtccaagcg gtggacaacg taaggtcgtt
    12781 gctgtttagt gtcgagataa gcagctaaat gattgagtaa ggtactgttt tctgtcggtt
    12841 catttggatg agtcttcatt cctgcaggct tatccactat aatcaaatgc tcatcttcat
    12901 ataaaacacg gatgtgcttc gcatctccta attgaacttc ttgataatta taatctgttt
    12961 cttcgaataa caatgtgatc tggtcattgc cctttacttc atgatggaac aaagcaggtt
    13021 gttgattgat ccaaacgttt ttacgtatac gcaaaaagtg tcgaaccttt ctaggaacta
    13081 accattcctt ttcaagcaag tcgcgaattg ttgtttcagg atggttcata ggtaaagtga
    13141 tcgtgatttt cacaagtgta ctcctttcag tgcaatcgca gactaattgt accattttta
    13201 aaacatattt aagaaaaaac ttacattttt ctgttcattc ttatgttact ctacttgcac
    13261 taacgtaaaa aaacatattg aactacaact taagcaggaa gaaaacactc tttcactatg
    13321 gtaaaataag acgattgaat caaatagaca ggagaagaag caaatggatt ttcaaagcat
    13381 tttagaaaag ataaaagctg ctttgatcag tttttggcgt tggatcagac catatctcgg
    13441 acaattccat cgatggcgaa agcgcatctg gaaaaaatat catgtgaaca aattgatttt
    13501 gctgattggt ttgatctgtg tcttagtgac aagtatctac ctctttattt tagcaaaaca
    13561 agcaaatgtc gaaacgttaa aatcaggatt aagccaatcg acagtcattt atgataagaa
    13621 aaacgaagaa gctggcacac tgtacgccca aaaaggaaca tatgtagaat taaacgcgat
    13681 ttctccctat atccaagatg cagtaatctc gacagaagat aggaattttt acgagcatca
    13741 cgggtttgat atcaaaggga ttgcccgtgc agcagtccga atggtcttgt ctggcggaac
    13801 atcagggggc ggaggaagta cgatcactca gcagcttgcc aaaaatgcgt atctgacatt
    13861 agaccaaacg tttgatcgaa aagcgaagga attatttttg gcaatcgaga tcgaaaagaa
    13921 gtacagcaaa gaagaaatct tgaccatgta tcttaacaat gcctattttg gcaacggggt
    13981 atggggtgtc caagacgctt ccagaaagta ttttggagta gatgcaacga atgttacgct
    14041 gtctgaggct gctactttgg caggaatgct gaaaggtcct ggtatttata atccgatcga
    14101 tcacccaaaa aatgcgaatg atcggaaaaa cacagtacta agtgtaatgg aagaaaacgg
    14161 aaaaatcaca aaagaacaag aacaagcaga aagccagaca gatatcagcg cgtatcttaa
    14221 tgacacatac gataattcag attctggtta tcgttatcct tattattttg atgcagtgat
    14281 cgatgaagca gttaacgagt atggtcttga tgaagaagat gtcatgaata aaggctataa
    14341 aatctataca gctttagatc aggattatca aaagcaatta gaagctactt atcaaaacag
    14401 tgcattattc ccagcaaatg cttcagatgg tgccatggtc caatctgctt cagtagcttt
    14461 agatccgaat acaggtggtg tacaagcact agtaggacga agaggagatc atgtctttag
    14521 aggattcagc tttgctacac agatgcgtcg ttcaccagga tcaacgatca aaccattaag
    14581 cgtatataca ccagcactgg aagctggata caagccttcc agtatcttga aagacgaacc
    14641 gcaaagttat tacgatgcgc ataattttga cgggacatac caaggagaag taccgatgta
    14701 tcaagctgtt gcacaaagtt tgaatttgcc tactgtatgg cttttgcatg agatcggttt
    14761 acaaaaagga tatgataaag cagaagaatt cggcctgcct ttagcaaaat ctgataagta
    14821 ttatggctta gcccttggtg gactggaaaa aggagtatct ccattgatca tggcgggtgc
    14881 ttacagtgca tttgccaatg atggtcagat gtatacgccg catcttatca cgaaaatcgt
    14941 tgattcgact ggagcggtga tcgtcgataa cacaaacaaa aaaccaaaac aggtcatatc
    15001 aaaggaaaca gccgatgaaa tgaccagcat gcttcttggt acattttcaa atggaacagc
    15061 ggctgctgcg aatcctgcag gttatacgat agcagggaaa acaggaacca cagaaactaa
    15121 tttcgatgcc acaaaagcaa atgaccaatg gatgatcggt tacacgccag acgtagtgat
    15181 ttcgacatgg atgggttacg aagaatccag caagctgcac tatttggaag ggacaagtgg
    15241 aaatgttgtc gggaaagtct tcaaatcagc ggcagaaggc atccttccgt atacttcaaa
    15301 aacaggattt agtgtagcag atgcctatgc cacaggagga caagtggtag ctgcagatca
    15361 agttggaaac acaggaaatt ctaatggaga gtctggaaat tggcaagata acttgaatca
    15421 atatggtgaa caagcaaaag aagggctgaa aaacttcggc gatatggtga aagaaggcgt
    15481 tcagggaatt ggtgaagcgg ctaaagacct ctggcggaaa tatcaaggag agtagcccct
    15541 ttgaaaacga ataaccccat gttacaatag agagtaacta ctaaaagaaa agagggcata
    15601 acatgtcaaa catctacgat agcgcgaatc aaatcgaacg tgagatccgt gaattaccag
    15661 aattcaaagc gttgaaagaa gcgtacgaga aagtacaagc aaacgaagaa gcacacaaac
    15721 tgttcaaaga cttccaagcg atgcaaatcg aattacaaga aaaacaaatg agtggacaag
    15781 agttcagcga cgaagatgca atgaaagcac aagaaatggc tgtgaaaatc caatcagaag
    15841 aagtaatcaa tgacttgatg caaaaagagc aaggcttcag tacaatcatc aatgacttga
    15901 accgtatcat catgacacca gttcgcgacc tttacagcga ataaaactat tttatgaaac
    15961 aacagagggt gtgaaagaga cgttcggcct tgagcgatga caaaattgct tcttattgcc
    16021 gataggctgt ctcttgaaca ccatttacca agaggtcgtg agcctaccgt ttaactctga
    16081 gttacaagga acaatttcaa aaaacagctt ctcatgtttg atgactattg tgacttgtga
    16141 ccgaggagtt ggcaggagaa caccgtttat tcagaaatag gagctacgac aaaagtcttg
    16201 tcatagctcc tattttttgt cattcatttt tcaagcggta gaaatagcct ttttgagcta
    16261 acagtttttc gtaagtacct atttcaacga tttttccttc atcgatcaca acgattttat
    16321 ctgcctcttt gatcgttgaa agccgatgag ccacaataaa agtagtgggt gtttctgtca
    16381 ggtaattcag ggcttgctgg atttttttct ctgactggtt atctaaagct gaggttgctt
    16441 catctagcaa aatgattttt ggctgacgta tcaacgctcg tgcaatggag atacgttgct
    16501 tttggccacc agacagcttg ttgccatgtt cgcctactaa ggtatccaga ccatcaggaa
    16561 gctggtcaat cagtgaagaa agttgcgcag cttcgatgac ttcatctaaa cgttcttttg
    16621 atacgttttt caagccataa gtgatgttct ctttgatcga agcggagaac aacaaagtgg
    16681 tttgaggaac aacggctaaa taatttcgca cacttcgcat atccagttca tccaaaggct
    16741 gatcatctag aaaaattttt cctgaagtcg gctggataaa tccaatcaat aagttaagga
    16801 gtgtagattt tccagagcca gatggcccaa caaaagcaat tgtttctcca gctttcacct
    16861 ctagagaaaa atgatcgata atcggtttga gtgagtccgg ataaagaaaa gaaacatcct
    16921 caaaacgaaa atttccatcc aaatgttgga tttttttctt accgtgataa gtttctacat
    16981 cgccagcaat caacagttcg ccaatagaag tgatagattc catgcctttt gaaatcgtag
    17041 gaatcagcgt gatcattcca gaaacggaat tgacgatcgt cgtgaaatac gtctgataca
    17101 tcacgacatc gccgggttgg atgcgccctt gtaaagctaa tatcccagtg aaaatcaaac
    17161 aaatcagctg aaatatctga aaactagccc agctgactga tccgaaaaca gattgcagga
    17221 tatctaagtg gaatcctttt tcatagattt gagtaaaatg cttatttaag cgagttgttt
    17281 cgtgtttttc taacccatga gctcgagcaa tcggtgctaa ttcgatcact tcgatcactt
    17341 ttgcagaagt ctcttccatt tcttgcctga agtctgagtt ggactgtctg atccttttac
    17401 ggaaaaagag agtaacgaat gcggcgagag gaacagtcaa taagaaaaac atgaaaacaa
    17461 caggactttt ataaagcgtg atacctaaag ctacgaataa attgacagcg atatttaatc
    17521 cgctgacaaa tacttgagag gacaacgttt ggatagcttc tacatcacgg atgatctttg
    17581 actggattcg tccagattgc aattcttttt gataaggaat agatagttct tggattttct
    17641 tgataagggt cgctcttagt ccagcttcta cttgacggat cgaagtgcta tggtatttta
    17701 catgaaaata attcgtcaat atattttgac caatcagaca agccatcaaa aaagcgttga
    17761 aataaatggc ctgcaactga gaagaatctt tcgatgaagc tgcattgatt acattggcaa
    17821 tagcgatcgg cattaaccaa gatggaagat gtttgataat aaaaaagagt cctgacatgg
    17881 caaaacgcca ataatttccc tgataaagat gcagcaatgt ctgaaaagca tttgcatgat
    17941 atttttgata acttttctgt attcgttcct ccataatctt ctccttaaca atcttggtaa
    18001 ataaactgca agtgttctat tgcttcagga acataaatct caggagtagc ttcaagagtg
    18061 gcgtacatta gaggacgctc atatttgata taatgcagta ccgggcggaa atctaaacag
    18121 cccttgccaa caggtaccat gcggatcttt ccattttcca tgatatagtc tttgagatga
    18181 agcgctgtaa tataaccatg taaattgtcc aaggcacctc ggatcacttt ttcctgatca
    18241 gcatgatttt ctttatgcat caaattcgca caatctaaaa tgatttttaa atttggtgaa
    18301 gctacttcat cgattaaacg tttagctaac tgatagttat agattggatg attgatccct
    18361 gcttcaacag caacagttac gccaaaattt tctgcatgag cgactaattc aatgatagaa
    18421 tttctggcga tctgataagc ttcttcagta aaattttctt ttgtgtaacc gacagagaca
    18481 ctaccggttt ccgtagcaat catcttggct tgataggcag agcacagagc aatttgctgt
    18541 ttgaaagttg ccaatgcttg tgcacgaact tcttgatttt tgctggatag gttgatatag
    18601 catcctaaaa tggaaagttc gatattgttt ttttggaagt attcaccaaa ataggcagca
    18661 ctcccaagtg tcatgtcagt taatttaagt tgttcaggaa atgctttttg tggtgcaaat
    18721 tggatatggg acaagtgaag ctgtgatact ttttgaacga tttcttcacg atttcgaaca
    18781 tctaaatcgt gggcacggat gcctaaattt aatggaaaca tctcaaactc ctttttgtaa
    18841 ataattagca agaggcagac gggattcttt tatcaattca acaattattc gggcaacttt
    18901 atcagcgcct tctggactaa agtgggtatt gtcttttagt ccttctgggt aatttttaca
    18961 agtatcaggt ataagatgca ggtagaattt ttttgtctct tcttttgaga aggtctggaa
    19021 caattcttgt gatcttgaga acacatctaa aagaggaata tggtgtttct cagcaaatgc
    19081 acgcatcgct tttggatagt ctccaagtat atctggattc aatgttccgt tttctaagta
    19141 atctcgtcgc gtcacagatg tcagtaagac catttggacg ttcgcttttt ttgcgcattc
    19201 aaaaaatatc tgcagatttt ttaaatagct accgaaggga agcgtacctc tttcttcttg
    19261 gattttctgg tcgttatgtc caaattgtac aaataagaaa tcattcggct gaaactgtga
    19321 agctagacgg gctaaccttc cttcctggat aaatgatttg gtgctgcgtc cattctttgc
    19381 ttggttattg atctggatct cgggtgaaaa ataagcagag attttttctc cccaacctgt
    19441 ctcgggacgc ttgttttcat ctttttctgc cgcagtagaa tcaccggcaa tatggatagt
    19501 aggcatgtta tctcttcttt cataaaggtt tataggcaat gaagcagaac agcaagagaa
    19561 gaagacggag caaatttggt ccgtcttctt ctctaaaacg tggataaacg gattccgtta
    19621 tttcactatc ctgtttttga ttcgttactg ttactattaa tcaattaggc aaaaaagcca
    19681 aagtaatttt tagcatttgt ataactaatc gcttgaacta gacttcctaa aagttgttca
    19741 tcttcaggtg cttctcctcg catgaccatt tcgcctaaaa attcacaaag aacacgacgg
    19801 aaatattcat gtctagtgta ggacaggaaa cttcttgaat ctgtcagcat gccgacaaaa
    19861 ttccccaaag cactaccatc agcaaattgt gtcaattgat cgcgcatacc agctcgagta
    19921 tcgttgtacc accatccaga acctaactgc agttttccat tctgttcttt ttggaaacag
    19981 cccatcaaag ctaaaatcgc aggatagtca ttcggattca atgaataaag gatcgtcttt
    20041 ggcaactgat ccgtttcttc tgcacggttc aataattttt gcagaggaat agtcaaagaa
    20101 agatcattca ttccatcata gccggtatcg ggccctaatt tttccaacat ttgtgtatta
    20161 cagttacgat aagcatggat gtgcagctgc atcgtccaat tatgagaatg atagaagtgg
    20221 gtcaaatgta tcagtgtttc tgtacgataa gcatcgattt catctgaaga taatagtttg
    20281 ttagctcgtg ctttttgaaa aatctcttct aatactgttt catccgcttc tttatagcgt
    20341 aagatgtcta aagcgtggtc ggaaacacga catccatgtt gatggaagta ttctatccgc
    20401 tgccctaaga cttctatcag ttcttgatag gtggtgatcg tttggtcagc tgcttcttcc
    20461 agtagtgtga cccatgcatt aaatccttct ttttcaatat tgagtgcttt atctggacgg
    20521 aatgaaggta gaaccttcaa atctggttct tcatgagcaa gcaactgatg atagtgcaag
    20581 tcgtctgcgg gatcatctgt tgtacaaatc acttctacat tcgatttttt tgcgatttcc
    20641 cgacggcgaa aatcaggagc tgccaaaatt tcatttgctt gtgtccaaat tttctctgca
    20701 ttttcttcat ttatcaacaa gtcaataccg aagaaacgct tcaattcaag atgtgaccaa
    20761 ctgtaaagcg gatttccgat aatttttggt acggtttgac accaagctaa aaatttttca
    20821 aaatcagaag catttcctgt gattttttct tcaggcacgc cgcaagctcg catcaagcgc
    20881 catttgtaat gatcgcctcc taaccaagct tctgtcagat tgctgaaatt gttgttttcg
    20941 taaatttctt ttggatctaa gtgacagtga taatcaataa tgggcatgtt ttttgcgtag
    21001 gaatggtaaa gctttttcgc ccaatcgtct tttaataaaa aatcatcaga taaaaacata
    21061 aatcttcctt ctttcttttt atactgtttt atgaaaatca aagtgttact ccgtttttaa
    21121 aaatggcaat ttctcgaacg tcattttgtt catttttcgt ttcttctcca ctagctacag
    21181 cgatgatttt ttgaacaaac gattctaaga ttacttccat tggttgttct aataagacgc
    21241 ctgcattaaa atccatccaa tgacctttgc gttcaaaaag agttgtattc gtagatacct
    21301 tgaccgttgg gacatatgag ccaaacggag tccctcttcc tgtggtaaac aagaccaact
    21361 ggcaatcgga tgaagcaaga gcagaggcag ctacaagatc attcccggga gcttgcaata
    21421 aactaagccc tggttttctg attttttcgc catattgtag cacatcaacg acaggagagc
    21481 ttcctgattt ttgcgtgcag cctaaagact tatcttctaa tgtagtgatc ccacctgctt
    21541 tattaccagg agatgggttt tcgtaaactg gttccccgta agacaagaaa tactgtttga
    21601 agtcattgat caagtcaacg attgagtcaa aaacttcttc attttccgct cgtgccatca
    21661 atacttgttc tgcgccaaac atttctggca cttctgttaa gatcgtgctg cctccttgag
    21721 caatcaagta gtcagaaaat gcaccaagta aaggattagc agttattccc gatagaccat
    21781 cagaaccgcc gcatttcaaa ccgacattca acttagagag cgggactgat atgcgctggt
    21841 cattttccgc cgctgttaaa agctcttcta atagagccac accttgttcg atctcatcgt
    21901 aaacatcttg agccactaga aatttcactc gttcttcatc atattcacct aaaatttttt
    21961 tgaattcagg aactgtattg ttctcacaac ctaatccaaa tactaaaact cctccggcat
    22021 tgggatgatt tactgcgtcc gctaaaattt ctcgagtatt ttgatgatct tttccaagct
    22081 gcgaacagcc ataaggatgc tttaaaatcg taatatggtc aaactgtccc agatctggat
    22141 gtttttcctt gaactgtttg acgatcagtt cagcaatgcc attcacacac ccaacggtag
    22201 gtactaaaaa caaatcatta cgaatcccta cttttccatt ttttcgcaag taaccttgaa
    22261 atgttcggtt ctcaaaaagg taatgacttg gatggacatc tttttcatat cgataatcca
    22321 attcaccaga aagattggtt tttacattat gagtgtgcag ccattgaccg gcagttacgt
    22381 ctgtcgtcac atgaccgatt ggatagccat atttgatcac gttgtctcca gctttcaaat
    22441 cacccaatgc aatcttatga ccttgcggga tatcttcaag agtggtgatc gtttgctgat
    22501 caatcgtcag ttgtgtttgt tttggaatag gggttaatgc gacgaccaca ctgtctcgtc
    22561 gatttaattt cattgttggt tgcagggttt caagcatgga ccatcgttcc tttcatttct
    22621 tgtaatactg accggacacc tttttgatcg atctttgcag ctatttcttg aactgtatct
    22681 gtcacattcg gtatttgaga caaatcttct ccccaaagcg cagcgttttg caataatcga
    22741 ggaatagctg ttccattatc agaccaagca tcagtaaatt cgcttatcgt cttttcatca
    22801 tcatgtggct ttacttgatc gcctcgatac ataagaagta gcgcagctag tgaaacagtg
    22861 attaacggtg gtacctgatt ttgttcttcg atatacttct tgaacactgg aagcagacgg
    22921 gtgctgaact ttgaaacact atttaatgaa atcgcttcta atttgtgatg ggcaaaagga
    22981 ttcaagaaac gttctttgat ttggtcagca taaatggcta attcatcttt aggtaaagaa
    23041 agcataggaa tcaattctag attgaataac tgatcaacat atgcggagaa taaaggatct
    23101 ttcattactt cttctacagt ttccaattcc gccaacaaag caagagggac catcgctgta
    23161 tgcggtccat taagcaaatg caccttacgt tcacgataag gagtcatatc atctgtgatg
    23221 atgacattca agccagcctg tttgaatggt agttgattct ccaattcttt aggcccttcg
    23281 atcacccaaa gcatgaatgg ttctgctttg accatcaatt gatcttcata gttatgccgt
    23341 tgattgaaaa cacctgcatc ttcgcgagga taacctggga cgatgcgatc aactaaacta
    23401 caacagaaaa cattttcttt ttctaaccac tcaacaaaag caggttctaa atcccatttt
    23461 tctgcgtagc gtaaaatgat ttcttttaga cgttcgccgt ttctgtcaat cagttcgcaa
    23521 ggaatgatgg taaagccagc tttgtttaat ttgaaacgtc ggtataaaaa ggctgtcagt
    23581 ttgcccggga agctctgtgg gggagtatct gttaaattat cagtctccac gaattggatt
    23641 cctgcctctg ttgtattgga gacgatatag gccaagtcgt cattttctgc tagagataga
    23701 taagcatccc attcatcata aggattgatc atttgttcta ctacggtgat aatctcggaa
    23761 gtatcaacga tttcgccatt gctgagtcct tgtaaaatga ccgtatataa attatcttgc
    23821 tggttcatca tatttccaag accttgttct aaaggttgaa tgattgcaac attaccttta
    23881 aacaattgtt ggttattcag ctgctgaatc tgccaatcaa taaatccacg cataaaattg
    23941 ccttcaccaa actgaagaac ttttacaggt gcttcgtttt ttttcactaa ttcttttgtc
    24001 agttttttca tataaacttc ctttctgtgt tatgcacacg ttaacatttt tatataaaat
    24061 tacttgtagt ttacaagtaa ttgatcgact cacgaaggat caacaaggtg gagagctcaa
    24121 ttttagattg ttcgatcgtc ctgccttcga tcaaatctag taaaagcttt gttccttctt
    24181 gacacatttc tttcaaaggc ttacgtacgg tggtaagcgg cggattgaga taggagacga
    24241 ctggagaatc atcgaacccg atcagagaaa tgttctgggg aacacggagg ccagctaaat
    24301 gtacagctct caatgcacca atcgctatgt cgtcgttgcc gcaaaaaacc agagtaggca
    24361 ggtcagaaag tgataatagg tattccatgt tttggaggcc gctttctaag ctgtaatctc
    24421 ctgttaaaaa ataggctgga ttgacaggga tgctcgcttt ttttagactt ttcaataccc
    24481 ctttttcccg ttcttttgtc gaacgaaaat ttttctttcc gccaatataa gcgatcttcg
    24541 tatgtccgca tccaataccg taatctactg cctgcataac accggccgca tcatcggcag
    24601 tgacgttgat aaccgctgga tcattgatgt gacgattgac aacaacgaaa ggaaattctt
    24661 tttctttgag atagtcgatg aaaggctgat catcatcact ttgactcata acgatcagtc
    24721 catcgtagcg ttgcaggttg atttctgtta aatcaacaac atcgatactt tcaacagata
    24781 aactgtaatt tgtgtctaat actgaatgga tgcctgcaat ggaatctgct aggaaatttg
    24841 atgaagttcc tttatgcaaa cttgagaaaa atagtccgat tagatatttt ttttgattca
    24901 ctaaaccttt tgcactatag tttggcgaat aattcatttc cttagctagt tgtcgtattt
    24961 tttgttttgt ttcatttttt attaaactgc tgttattcaa agcacgagaa actgtcgtat
    25021 gagaaacgcc agctgcttta gcgatatctt tgatcgttac cattccatca ttttccttac
    25081 tttttcactt tttgttaacg cattcatcat attacgataa ctacgataag tcaatagatc
    25141 tttgatatga aaagattgca cacgttaaaa attacttgga tttttatttt taattatgct
    25201 attcttgaaa gcggggaatg ctcatttaat tagatgagta tcaattttac agaaaaatga
    25261 aagcctttta ttttaaaagg aggagaggaa gaaaatgaag aaaagtacag tattttataa
    25321 aatattttta ccgatgtttt tattaggagc cgtacttgtc ataggattta gtttattcat
    25381 ttaccagaat acctatgaat cgatcgaatc gagttattta acagacaaaa agaatttgct
    25441 caggcaagtt aaaactaacg ttgaatggaa aattagaacg attgaatatt cgttttctac
    25501 gtatggttcg accaaaaatt tctcggacat attcaagcat ccgctgacat atacagatta
    25561 ctccacttat tctgaaatca ggaaagaact aaattttata gaaacgattg ttatggatga
    25621 taataattat gatttagtca gtttggctgg taaatgggga gtaatcaatg gatcgttgag
    25681 tcaactgacc gaagaggaag ttgaaaagta tcaacagaag tacataaata acaaaaataa
    25741 tttattttgg caaaagaaaa acaaaggaat cgaaatggtg atttctttgc caatgtttga
    25801 aaaagaaaaa tatgcattgg gaattgctac gatagagaaa catacaatag aacaagttgt
    25861 tggtgaaagt ggagaagaca tactttctat tgaaaacgaa gttggcaacc tttttactac
    25921 tcagacagta aaaaataaag agcaaccaac tgtattaacg gacgtggatc tagattacaa
    25981 tcagctgaaa gtgataagag aaaaagataa aacgtatata gtactgaaat cagattataa
    26041 taattggatt tacagattag aagttgactc tagtgcaatt ggcgcaatga tccgcaatct
    26101 gagaatcggg ctagttgcag tgtctttaac actggtagga ctcattggtc ttctttccta
    26161 cttattttcg gacagatttg cccgtccgat ttctcaaata caggaacgtc tgaatcttaa
    26221 atcaaaagga ttcagtggga aagaactaga tttggtcgct caatcagtca gtcggatcat
    26281 aggagaaaaa gaagcgttaa gtgcacactt agttacccaa aagccacagc tggaaacgct
    26341 atttgtactt agtttattta gaaatcgagt agaaaggcga gaactagatc agcggttgca
    26401 gcaatttggt tatagttctc aaaatgactg ctattataca gctttggttc aaatcgatct
    26461 gttagaagat agtcacggtg gggagcgtga tttgctactt ttagcgatca acaacatgat
    26521 cacggaaatt gtgccaaagg aaaatcgaat gctgccgatc gttttgaatg aagaaatgca
    26581 agccactatt tatcgaatgg ataaagatga tgcagaagca agcaaaaaag tcatggagta
    26641 ttgccgactc attcaaaaac gtgtaaaaga atacttgaat ttaactatca gtataggcat
    26701 cagtaaccgg ttcgaaacgc tgaatgaaag caagcaatcg gttgatcggg caaaagaagc
    26761 cctgtattat cgtgtaaata ctggtccaag ttcaattatt ttttatcagg aaatcgttcc
    26821 tgctgctcat gaaaaaacac ttattagata tccgatagaa gaacaaaatc gattgtttga
    26881 agccattcgt tctggaaatc aggaagtgaa gaatttagta catgaattga ttgatgcatt
    26941 gtttgcacaa aataaaaatc ctttgagtcg ggaagtagtt tcggtccgct tgatcaatga
    27001 actcgttcaa ttaggacagt tattgggagt agattcaaaa aactttgata acatgaaaca
    27061 aatttatgta aaagcactga acaattatca tcccaaagaa ctagaaaaac ttgttttaga
    27121 acaattagtt ctgccaatca cagaatgctc tcaagcaatc actgatcatg aatttaaaaa
    27181 tttgtcggag aatattatgc atatcgtcta cacagaatat gatcgtgatc tgtctttaga
    27241 tgtgatcgct gataggttgc attataaccc taactattta agtaatgtat ttaaaaagga
    27301 aacaggagaa aaatttggtg attttgttca aaatcatcgt ttagaggtag caaaaaaatg
    27361 gttgaaagag actaacctct ccgtaaaaga gattgcagaa agattgcaat atcgaaatcc
    27421 gcaaaatttt attcgtttct ttaaaaagaa ggaatcgatt acgccgggag aatatcgaaa
    27481 aaaatatttg tgaatcaaga ggctgttttt cttgtggact gattcaagca ataaaagaag
    27541 ttagaataac cctagtattt gtatttatgg atagttaaat ttcaaggcta agcacgcaaa
    27601 aagaactgtg acaaaagtct tgtcacagtt cttttttacg aataaatggc gttcaagaga
    27661 caggctatcg acaataaggc ataattttgt gaaatatggg aagctatttt tcaaaatcgc
    27721 ttcttattgc tcctgtgggt tactcgtcgc aatgtttgtg agagttttta tcatcgctca
    27781 aggctgaacg tctctttcac aacctctgat acacggtgtt ccaggagtag attcttcgaa
    27841 aaataagccg aaatttcaca aaaatttaag aagcaatttt cgtaaatgcc ttcttatttc
    27901 ttgaagctga ccacttctga cacaactacc attttgcgtt cagtacataa tcaccctttg
    27961 acagaaccta cgagtgctcc tttgacaaaa tatttctgaa caaaaggata aacaatcagc
    28021 ataggcactg ttgcaataac gataacagcc attttaactg tttgtgctgg tggaatcaca
    28081 tcaacggcac tactttctgc ttgcatccca cttgatacaa tgacgatttg gcgtaaaaga
    28141 atctgtatcg gccatttaga tgaatcgttg atatacaaaa tagcagtcat ataagtgttc
    28201 cagtaagtaa cagcataaaa aagcgagata gtagcaatag acggtaacgc taatggcagc
    28261 ataattttga aaaatacacc aaaatctgtg caaccatcca tttttgctga ttcttccaaa
    28321 ctatccggca aggcttggaa aaagtttctc ataatgatca tattcattgc attaacagcc
    28381 acaggcagga tcatggccca gtaagaattg accagcccta gagaacgaac aactaagaac
    28441 gttggaatca tccccccgct gaacaacata gaaaatacaa caagaaagtt gacgaaacct
    28501 cgaccaaaca aatactttct ggaaagaccg taggccatca gtgaagttgc acacatactg
    28561 acgatcgttc cgacacctgt aacaccaatc gaaacagcaa gtgctctaaa aatggtcggc
    28621 gtagaaagaa tgtagcgata cgcgtctaaa gaaaaggtag taggaaaaag gataaacttt
    28681 ttcgcgacga cttcttgtgt agaagcaaaa gatgtagcga tgatatttaa aaaaggaacg
    28741 atacagatca aagctagcaa aattagaaac gtattgttga aaagtgtaaa acttctttct
    28801 cctatagaga ctttcttttt catatagata ggctcctcct caaaataaat taaatgtttc
    28861 tgtttctata tgttcaattt atcatcggtg aaagattacg tatataatca tttcctatgc
    28921 taagtgtcag atagaaaaaa atagaaggtt tgctatgcgt agtttatttg tgaaaaaata
    28981 cactttatcg ttttgtatag cacaaaaatc attgctatgt aagcgttttt acttacggta
    29041 aaaatgatta tatacgtaat tcagatagtt atctatcatg aagtcataag cagcaaacat
    29101 tcacaaaact aaggaggggc ttatggaaag tgtaaagaaa caagaaccat ttcaactgac
    29161 gctatcgaaa aaagaagaaa agctcagaag acgaaaaaaa ataattgcag gaatcaaaac
    29221 gaataaattt ttatatgtga tgattctgcc gggagtcctt tatttcttga tttttaggta
    29281 tcttcctatg gggggactcg ttattgcttt tcaagattat caaccatttt taggaataat
    29341 gggaagtcct tgggtcggtt tcaaacattt cattcgtttg tttacagaac cgacattctt
    29401 tacattatta agaaatacgt tgatcttgtt cggattaaat atcttcgtgt tttttccgat
    29461 gccaatcatt ttgtctttac tattaaatga agtaacgaac aaacgattga aaaacgggat
    29521 tcaaacgatc atttacatcc ctcacttcat gtcttgggta atcattgttt cgattactta
    29581 tgtatttttg aatgttgacg gaggggtatt gaatgaattc cttgcaacaa tgggatttga
    29641 aaaaatcagt tttctaacat cccctgaatg gacacgcaca gtatatatcg gacaagtgat
    29701 ctggaaagag ttgggctggt ctacgatcat ctacctttca gcgataactg cagtcgatac
    29761 acagttgtat gaagctgcag aaatggatgg agcaggacgc ttacgcaaaa catggcatgt
    29821 tacattgccg gcaattcgtc cagtaatcat taccttgctg attctaaaaa tcggtcagac
    29881 attagactta gggttcgagc atatgtactt gttattgaat tcattgaatc gaagcgttgc
    29941 agaaattttc gacacctata tttatacagc tggattgaag aatggacagt taagtttcag
    30001 tacagcaatg ggattgttta aaggggtcgt aggattgttt atggtaattt tagctaataa
    30061 gattgccaaa aagctaggag aagacggcgt ttactaagat agaaagagag ggaaagatat
    30121 gctgcagtca ttagataaaa taaacggaag gctaaatcgt attttacagt tggtttattt
    30181 gaattttctg tggggcggtt tagtcatcgc aggtttggga gtttttactt tcggtcctgc
    30241 atcgtatgcg atggttgcag tgattagaaa atggctgcgc tcaaaagaaa cgtttccttt
    30301 gacgaaaacg tacttcacat attttaaaga aaactatcgt gaaaccttgc tgatgagctg
    30361 gatatatggc gccatcggga ttgttttact tgtggatctt tctttcatac acaattggta
    30421 tcttaggatc gctttgtata ttgctgcgtt catatatgtg ctctcagcac tctatatttt
    30481 cccaatcatg gctcattatg attggaaagg atattttttc aaaatgaaaa tggcattttt
    30541 attcggattc tcacatcttc attactcatt agtgttgata ttaagtatgg tagggattta
    30601 tctgatgatc atccaaacaa tgccaggtgt tttaaccttt atcggtggca gtctcttctt
    30661 ttccgcagtc acgtggacag caaattctat attttgccat ttggaatgga agaactcata
    30721 tacagaaaaa acagaagaaa acaaaaatat tattaaaaca gtgaaggaga aaatttatga
    30781 aaaaagcaac gctcgtaaaa ttaggtctta gttttgcagg aattctagca cttgccgcat
    30841 gcggcagcag ttcaagtaac agttcttcag agacagaaga tggaaaagct aaaatcactt
    30901 ggttaaatat tttgcatcat gcttcaccgc ctacagacac gattgtgaac ttgatcgaag
    30961 aaaaaaccga tacggaactg acattcaact ggattcctga tgcttcaaaa gaagaaagat
    31021 taacaactgc attggcttct gatgaactag gagatatcgt tacacttcaa attgcgatgt
    31081 tgaaaaactc ttctgtccgc aatgcgttaa aatcaggcgt attctgggat gtatcagaat
    31141 atttaaaaga ctataaaaat ttaagtcaaa tttctgaaga tagaatcgaa gctgcgagta
    31201 tcgacggtgc cttgtacggc gtaccgatac aaaaagacta cgctcgtgct ggcttagtca
    31261 ttcgaaaaga ctggttggat aatctaggac tagatgttcc aactaccatg gatgagcttt
    31321 atgaagtagc taaacaattt acagaaaacg atcctgatgg aaatggtgtg gacgacacag
    31381 ttggttttgg cgatcgtgcg cctagtgata ttcgttatac cagctttaaa cttttcactt
    31441 cttattttgg agcaccaaat ggctgggctg tagacgataa tggcaaattc actccagaat
    31501 ttgatacaga agagtatatg aaagctttga atttttcaag agatctattt gagaatggct
    31561 atttggcaca agactttgca gttacccaaa agactgatca gcaagaacaa tttgcacaag
    31621 gaagaactgg aatctacact ggaatggtgg atatcaagaa tctgaagacg atgggtcaag
    31681 gtattcaaga tgatatggaa ttagtgcctg taaacaagat ctcagatggt gaaggcggcg
    31741 gttatcatgt ttggtcagaa ggaaatggta ttggcggctt gctagcattt cccaaatctc
    31801 aagtaaaaga cgaagcacaa ttgaagaaat tacttcaatt tgttgatgat cttttagaag
    31861 aagatgtgta taaattgatg actggcggta tcgaaggaac gcattatgaa ttgaatgatg
    31921 atggttctat cgaatttatc gatatggatc tttggcaaca agaggtgcaa ccgttatcaa
    31981 gctctcgtcc tagtgaagtg acgttctctt tcaaagatac agaccctgaa aaagaattct
    32041 ctaatcaatt gatcaaagaa aatgaagaat ttgctgtaat ggatccttct gtgccattgg
    32101 attcaccgac gaacaatgaa gtaggatcag aaattgaaaa aatcgtcatt gatgctacgg
    32161 tgcaatacat catgggacag cttgacgaag atggattcaa aaaagctgta gaagactgga
    32221 aagcacaagg aggcgatcga atcacacaag agtacgagga agcatacaaa gcagcggcac
    32281 agtaatatag acgattggga caaatacttc ttggtatttg tcccaaatcc tattttaatt
    32341 tttaaaggag agcaaataat ggaaaactgg tcaaagaaat tagcaaaaag tatcatggaa
    32401 agaacacctc gcttatatga agaaaagtgg tataaaggaa aatggtctta tgactatggc
    32461 gtcgtactta aagggttcca attactttgg gaacgaaccc aagaaaaaat ttattttgat
    32521 ttcataaaag acaatatcga ctatttcgtg caagaagatg gaacgatccg tggctacagc
    32581 gttgaagaat acaatatcga ccatgtaaat acaggaaagc ttttcttttt attgtacaaa
    32641 gaaacaggcg aagaaaaata taaaaaagca gcagaactcc tttcccagca gttggcaaac
    32701 catccacgca catccgaagg agctttttgg cataaagaaa tttatcctta tcaaatttgg
    32761 ttggatggct tatatatggg ttctcctttt tatgctgaat ttatccagaa ttttagtgaa
    32821 gaaaaagatt accaagatgt tttgagacag tttgaaatca gttataaaca tctattcgat
    32881 ccaaaaacaa atttgctgat tcatgcatgg gatgaaaaga aggtccaacc atgggctgat
    32941 ccaaaaacag gtctatccca tcatttttgg agccgatcga ttggctggta tttgatggct
    33001 gcagcagata cctatgaatt acttcatgaa gaaacagatt gttctttgct gaaagaaatt
    33061 ctagaaaaga cactggctgc attacttcca tatcaagaag aaaacagcgg gacatggtat
    33121 caagtaacaa cagaaacaaa acggaaagga aattatctgg aagcttccgg cagtagtatg
    33181 tttgtttacg caatggctaa aggaatacgt ttgggactgc tgaacaaaga agaatggctt
    33241 ccacaagtga agaaatcaca tcaaggatta cttgatgaat ttgttctgga aacaaaagaa
    33301 ggctggctga acttgaataa aaactgtcaa gtagcagggt taggcggagc agatcagcga
    33361 gatggttcat atgcttacta catcagtgaa ccaattattt gtaatgatca aaaaggcgtt
    33421 ggtgcattct tgcaagcatt gattgaagtg gaggcattat aaatatgtat gatattttga
    33481 catttggcgc atcaattgat gaattgaata ctgaagcaat acaacaagcc attgatgctg
    33541 cagcaagtga cggaggcggg accgtagttg ttccagcagg tgaatttcta acaggagctt
    33601 tatttttaaa aagcaatgtc gaacttcatt taagcgcagg agcggttctg aaattctctg
    33661 acgatccaaa agattatcct gttgttcatt cacgttggga aggagtccat cgaaaagttt
    33721 atgcttcctg tatctatgct caaaatgtag agaacatctc tgtcactggt ttcggtacat
    33781 tagatggaaa tggaaaaaaa tggtggcata cttttagaaa tgaaccagat aatctcgctt
    33841 atcctcgtcc taaattgatg agtttccata actgtcatcg aattactgtg aaagatatta
    33901 aattgatcca gtcaccaagt tggacgatca acccgattct ttgcagtaat gcaacgtttg
    33961 ataacttgac gatcttgaac ccagcggatt caccaaatac agacggaatt gatccagaat
    34021 catgcaaaaa tgtgcgtatt agtaattgcc atattgacgt gggagatgac tgtattgcaa
    34081 tcaaggcagg gactgaagat acgtacgaaa ggatcgcttg cgaaaatatc acgatcacaa
    34141 attgcacgat ggtccatgga catggcggtg tcgtattagg aagtgaaatg agtggtagta
    34201 ttcgtaatat taccatttca aattgtatct tccaagaaac tgatcgagga atacgattga
    34261 aatctagacg aggtcgaggc gggatcgttg aagatattcg agtatccaat atagtcatgg
    34321 ataatgtgat gtgtccattt atactgaatc tttattattt ctgtggtccg cgagggaaag
    34381 aaccgtatgt atgggaaaaa aaggcttatc cgatagatga acggacacca gcattcagaa
    34441 ggatccattt ttcgaatatc acggcaagga atgtccatgc ttctgcaggt tttatttatg
    34501 gattagcgga acaattcata caagaaatca catttgacaa tatcgatgtc tcgatggccc
    34561 aagatgcagt tcccggtgtt cctgctatga tgacaggaat agaggaaatg agcagacaag
    34621 gattttatat tggctttgcg ggtaaggttt cctttaacca gatgtcgatt gaaaatcacg
    34681 agggaccagc atttctatta gaaaatacta aagaaatcag tattgagaaa ttccaatcaa
    34741 atcaatcaga aaatatggat gaactggttg ttcgtaaaaa ctttgcttat gcagttgaat
    34801 gattttttag gtgctccatc tagcagacat acccctagtg gaaaacgatt ataccgagaa
    34861 aaacaaaacg gttttatgct tgaatactat caattgtact taaatgaatt ggaaacagta
    34921 ccagccctct atgcttatcc agaaaaagaa ggaccgtttc cgccggtcct ttacctgcat
    34981 tctcacggcg gagatttttc agtcgggaaa agtgaattga tccatggtgc tccttactta
    35041 gcttctccat cttttgcaga agtactgaca ggaatgggat atgctgtttg gtcgattgat
    35101 gcttggggat ttgaagaacg cggaggtatt tctgaaagcg agttgttcaa acaattttta
    35161 ttaacaggaa aaacgttatg gggaatgcgt atttatgatg tgatcagctt gatcgattat
    35221 ttggagacaa gggaagacac agatactcaa cggctcgcaa cgattggtct ttctatggga
    35281 ggcttattaa gctggtgggt gacagcattg gattctcgtg tcaaagtttg tattgatcta
    35341 gcggcacagg tggacatcga aatacttttg ctacatcgcc gcctagatca tcatgggttt
    35401 tattactatg tgccaaatct attgacagct tatacaacgt tagcaatcca agaaaaaatc
    35461 gctccgcgcc ctcgtcttag cttagttgga aaaaacgata cgatgtgctt ggacgaaggc
    35521 gtactgaagc tgagaaaagg gctggacaaa aagtatcaat caatgggtag tccagaaaac
    35581 ttttcttctt tttctttaac aggcggtcat atggaaacac aagaaatgag aaatatttgg
    35641 aaacaattca taagggagca tttatgagga tgaaagacaa actgataatt ggaaagaatg
    35701 ctatagatac cccgacggat tttgaaacta tccaagaagg aatcgattat ttggcaggcc
    35761 tgccggatag ccatccaaag gtcttgatag taaaagaagg aagatatgat gaatatatag
    35821 aaagcagatt atccaatttt aaacttgtgg gagcaggaac agtagttatc tccggtaatc
    35881 gttttgcaaa acaggagtat cttgacggta gccagttagg gactttccga agtgctactt
    35941 ttttcttaga aggatcgaat gtcgtattag aaaatcttca catcgaaaat acagcaggtc
    36001 caggagaaaa ggtaggacag gctgttgctt tattcaattc aggagatcgc gtatctttaa
    36061 aaaactgtcg actatctggt tatcaggata ccttatgcac gggtccgttg cctgatttgc
    36121 aaaaagatgg tactcctttt tgtacaccgg cttctgccaa tcctgagtat tgtcgtcaga
    36181 cctatgaata ttgctggatt gaaggaacgg tggatttcat atttggtggg gcagatgccg
    36241 tttttcatca gtgtcatatc catagcttag ccagtaaaaa tcctggatac atcacagcag
    36301 catctacgcc gaaaaatcaa aaaacagggt atcgttttga aagctgtacc ttgactgcag
    36361 aacctgatac tagaaacgtc tatttaggaa gaccgtggcg tccgtatgca aatgtcttat
    36421 ttgaagcgtg taagataggt gggcatgttc acccaactgg ttgggataat tgggatgaca
    36481 agaaaaatga acagactgtc aaattctctg aacgtaataa caattatgaa acatcgatca
    36541 aaagacctga atggatacat ttagaaaagc agggggttca gaaatgaaaa gaaaatggga
    36601 actattgcta ggaatggtcg gtggcagtct atctttgatt ttctttggcg ggttagcagt
    36661 tactttaagt aatatgtctg cgtcagagtt taaaaaaagt tatcagtcgc ttgctgtaga
    36721 tcattcaact ttgtctttag aaaatacatt tggattatta caggacatga ctggtctgtt
    36781 tgccgttgtg ttgtttattt cacttgcttt tttagcagta gcattgtttt tgactgctaa
    36841 aggaaaatac ttaacaactg ccactggttt gtattttata acaggattca tattgttgat
    36901 tggcactcag tttattgcgt ttccttttgc atttttttat ttcgcagctg gggcattcag
    36961 tttatatcga gtaagaatga gaaaaggagc ctgaatcatg cagactatca aagtgaaaaa
    37021 tcctatttta agaggattca atccagatcc taatatatta agagtgaaag agcggtatta
    37081 cattattgtc tcctcttttg aatggcttcc aggaatccga gtatatgagt cgaaaaattt
    37141 agtcaactgg tcacatacaa cggatatttt gaccactcaa gtatctttgc aaggaaatcc
    37201 tacaaatgga agtatatggg cgccacaaat cagttatgct gatggattgt tttatcttgt
    37261 ttacacagat gtcaaaagtg caggccgtcc gtttaaagat gtccacaatt atttgatcac
    37321 tgcatctgag atcacaggtc cttggtccga accaatttat ttaaatagta acggctttga
    37381 tccttctttg ttccatgatc ttgaaacggg aaaaaaatgg cttgtcaatg aactatggga
    37441 ttatcgtctg gacacaccga ataaatccag cggtatcgta ttacaagaat atgatgcaga
    37501 gaagaagaag cttgatggtc ccatctataa aatatttgca ggtacagaat tagcgaaaac
    37561 ggaggcacca catttgtatt ttcgtaacgg gtattattat ttgatgacgg cagaaggcgg
    37621 tacaggaaga ggacattcag ttacagtttg ccgagcaaag aatattactg gtccttacga
    37681 gcttgatcct ggttttccta tgctgacagc tagtgacaaa cctgcttcta cattgcaatg
    37741 tacaggacat gggagcttag tacaaacccc gtcaggaaga tggtacatga catatctttg
    37801 taccagacca ttaaacggtg cggctatttt gggaagagaa accgctattc aagaagtgta
    37861 ttggacgtcc gacgattggt taagacttaa agatagccag acaactccga tggaagaaat
    37921 aatgattgag acaaatgaag aggtacaaca agaaaaagat catcagttta tggatacatt
    37981 tgaaggagag ctaaagaaga actggaatgc cagacgcatt ttgccaaatg ctgattggtg
    38041 tgatacaaaa tgtagaccag gttttttacg tctgatttcc ggtgaatctc ttcaatctac
    38101 cttcgaacat catttgttag ctattcggca aacggatttt acttttgatg cggagacagc
    38161 atttgaatac atgcctaata attttaatca gatggctgga ctggtactgt acttaaatga
    38221 atccaattat atttattgtt atgttacttg gaatgaggac aagggaaaat gcctacgtat
    38281 gatagaatca gaaaacggag tagcacaaat agaagattgg acgattccac tcacagaaaa
    38341 gaagactttt ttgaaagtca ctgtaaagaa agagacagca cagtttcatt tgaaagacgt
    38401 atcaactgaa gaatggaaga aagtaggtcc gcaaaaaaac atgtgtatac tctcaggagg
    38461 gtttactgga aattttatcg ggatagcagt gcatgatcta aataaaaaaa gaggaagtta
    38521 tgctgatttt gagtttttca agtatgatgg aaaagatcat ctgtaattta attctttaat
    38581 aaatgaggtt cactaactta ttttcactag ttagtgaatt tttttgtttg tatatattgt
    38641 tatgtaaaaa aagttatcaa tcttatgcat caatgttttt agattatttt ttgtttttta
    38701 tctgtcaaga taatgcaata aaaagccaac attgtgaata taaaaacaag aaaaaaagag
    38761 ttataatttt gaaaacgttt actagaagag gtaggaggaa ttcaaatgct ttcacaaaat
    38821 gctgttacag caaatacaat aaaaaaagaa aaaaaggaat ttgggatttc cgataagata
    38881 ggctacatgt tcggtgatct aggtaactgt tttattttag gtttagtcaa tagtttcttg
    38941 atgatttatt atacgaatgt tcttggcgtt tcaggggcaa tcgtaggtac gttattcttg
    39001 atttcgagaa ttttggatgc gtttgctgat gtgatggttg gaagattatg tgatgtctca
    39061 gagttaacag aaactggccg atttattcct tggatcagaa aaatgaagta tccattttgc
    39121 ttagtgactg tcattttgtt tttgccattc gtccaacatc ttccaatgac ggtgaagatt
    39181 gcttatattt ttgtttcata cattgtttat gggatttttc tctcaacaat caatatcccg
    39241 tatggagcaa tggcagcagc aattagctca aatcctaatg accgcacctc gttgtccacg
    39301 ttccgaagca ttggttctgc gattggcgga tcaactacag gattcttcat tccgatttta
    39361 atgtataccc ataacaagag tggacaacaa gtcgtttcag ggaatcattt cttttggatt
    39421 tcaatcggat gtgctgcaat agcatttgtc gcatatatgt taacttgtaa attgactact
    39481 gaaagagtaa gagttgtcaa aaacgaaaaa gtatctgctt ctcagttggt aaaaggtttg
    39541 ttagctaatc gagcactgat tattttagtc attgttgaca ttttcattgt gatcaatcag
    39601 attttagctg ggacgaatat gacttattta ttcaatgatt acttccataa taaacaagca
    39661 atgtctgttg ctcttttatt tacttacgga acagtagtag ttttagcacc atttgctaca
    39721 aagctgacaa aattatttgg taaaaaggaa gcaagcgtat ttgcgttact tgcttcttca
    39781 tgtatgtact tgctgatgta tttccttcat ataacaaatg catgggtata tctgattctt
    39841 ttatttatcg ctactttagg atctggttta ttcaatttga tggtctgggc attcattact
    39901 gacgtgattg attatcatca atataagaca ggcttaagag aagatggaac agtatatgga
    39961 gtaaattctt ttgcgagaaa agtaggacaa gcatgtgcag gagcggtagg cggatttatg
    40021 ctggcactca ttggttacca atcgtctaca acaggtggag ccatccaatc agttttagta
    40081 caagaaagga tttatacgat ggcaaacttg ttaccagcat tctgcttatt tgtatccgca
    40141 atcattttgc tagtaggtta cccactgaat cgttcaaaaa ctgtatttat tggtgaagaa
    40201 ttgaaaaaaa tcaatgaaag atagggagaa acgtattgcg gacaaaaaaa gggattatca
    40261 atgctttgaa ctttaaaaca aacgacactc aacaagatga tacaataata ctgcaaaaag
    40321 caatcgatca aggtgcgaaa gaacacttac ctgtatttat tccaaaaggc atttatttag
    40381 tcggagccct ttttttaaaa gataagagtc atcttatctt tgaagaaggt gcagtcctca
    40441 aggggagaac agatatcgaa gcatttccag aaatcgatac acgagtagcc ggcgtagaaa
    40501 tgaaatggcc agcagcgatt ttgaatgttc tctcggcgaa agatattctt atagaaggaa
    40561 aaggaataat cgacggtcaa ggagaccatt ggtgggagct gtattgggga aaagatcaaa
    40621 aaagcgggac aagagctgaa tacgatcaaa agggacttcg ttggattgcg gattatgcta
    40681 ttaaacgtcc acgcgcttgt ttactgtatc atgcagaaca tgttgttgtt cgtgatttga
    40741 tttttcagaa atcaggtttt tggaacttgc aaatcactta ttctaacgat gtattagtag
    40801 aaaaagtaat catccgcaac aatgatggac caagtacaga tgggattgat attgattctt
    40861 caacaaatgt gcgtgtctat gaatgtgatt tggcctgtgg tgatgattgt attgctatta
    40921 agtctggcag agatggcaat ggtgcaagag tcaatcgaaa atcatcaaga atcgaggttg
    40981 ctagatgtaa aatccgttca ggatacggtg taactattgg tagcgaagtt agtgctggag
    41041 ttagtgacgt atatattcac gatatcgatt tttttcagtc agactgtggt attcgaatga
    41101 aatcttcaaa agaaagaggg ggagtgatcg aaaatatcag ggtagaaaat ctgaatatga
    41161 ttgatgtaca atttcctttt tcttggataa tggactggca taatgaatac aatagaaaaa
    41221 atagcaatga ccttgctcaa atgccagaaa gttggcaagc agtggctgaa gaaatacctg
    41281 aatcaaagca aatgacgaaa gtcaaagaca tacacatcaa aaacgtacag gcatgccttt
    41341 ctccaggtta ctcacttcct tcccgagctt ttgatttagt agcatttcct gaaaagccta
    41401 tagaagatgt ctgcttttct cattgccaaa taactgcaaa agaattcggg cgaatcgaag
    41461 cagtacaaaa cttatgtttc aaaagctgtg atttatctat agaaactggc aacacagctg
    41521 caaacaatac ttttgacaat cgttgaaatt ataataaggt caagcttaaa taatatttta
    41581 gcttgacctt tatttttttt aatgatatat taaacaacgt tgtcgcgctt cattgaaaca
    41641 ttttatgaaa attccaaaaa agagttgaca catgttttta gacgtgataa gataactaag
    41701 tcgttaaaaa gaaccacttg cgaagcttga ttctatcgaa aaaaacttca aaaaagttct
    41761 tgacaaataa caaacgataa gttatga
//
LOCUS       DAZBTF010000017        41703 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000017 DAZBTF010000000
VERSION     DAZBTF010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.017"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..617)
                     /gene="dhaL"
                     /locus_tag="ACHGNS_000989"
     CDS             complement(12..617)
                     /gene="dhaL"
                     /locus_tag="ACHGNS_000989"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFN6936952.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(645..1655)
                     /gene="dhaK"
                     /locus_tag="ACHGNS_000990"
     CDS             complement(645..1655)
                     /gene="dhaK"
                     /locus_tag="ACHGNS_000990"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFN6936953.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(1630..2025)
                     /gene="dhaM"
                     /locus_tag="ACHGNS_000991"
     CDS             complement(1630..2025)
                     /gene="dhaM"
                     /locus_tag="ACHGNS_000991"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFN6936954.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            2179..2499
                     /locus_tag="ACHGNS_000992"
     CDS             2179..2499
                     /locus_tag="ACHGNS_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFN6936955.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(2611..3468)
                     /locus_tag="ACHGNS_000993"
     CDS             complement(2611..3468)
                     /locus_tag="ACHGNS_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFN6936956.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(3576..4880)
                     /locus_tag="ACHGNS_000994"
     CDS             complement(3576..4880)
                     /locus_tag="ACHGNS_000994"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFN6936957.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(4877..5386)
                     /locus_tag="ACHGNS_000995"
     CDS             complement(4877..5386)
                     /locus_tag="ACHGNS_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936958.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(5561..7696)
                     /locus_tag="ACHGNS_000996"
     CDS             complement(5561..7696)
                     /locus_tag="ACHGNS_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFN6936959.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(7779..9434)
                     /locus_tag="ACHGNS_000997"
     CDS             complement(7779..9434)
                     /locus_tag="ACHGNS_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFN6936960.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(9436..9750)
                     /locus_tag="ACHGNS_000998"
     CDS             complement(9436..9750)
                     /locus_tag="ACHGNS_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFN6936961.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            9920..10180
                     /locus_tag="ACHGNS_000999"
     CDS             9920..10180
                     /locus_tag="ACHGNS_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFN6936962.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            10194..10508
                     /locus_tag="ACHGNS_001000"
     CDS             10194..10508
                     /locus_tag="ACHGNS_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFN6936963.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(11238..11792)
                     /locus_tag="ACHGNS_001001"
     CDS             complement(11238..11792)
                     /locus_tag="ACHGNS_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFN6936964.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(11918..13847)
                     /locus_tag="ACHGNS_001002"
                     /pseudo
     CDS             complement(11918..13847)
                     /locus_tag="ACHGNS_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(14153..14869)
                     /locus_tag="ACHGNS_001003"
     CDS             complement(14153..14869)
                     /locus_tag="ACHGNS_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFN6936965.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(14870..16693)
                     /gene="glpO"
                     /locus_tag="ACHGNS_001004"
     CDS             complement(14870..16693)
                     /gene="glpO"
                     /locus_tag="ACHGNS_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFN6936966.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(16707..18203)
                     /gene="glpK"
                     /locus_tag="ACHGNS_001005"
     CDS             complement(16707..18203)
                     /gene="glpK"
                     /locus_tag="ACHGNS_001005"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFN6936967.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(18339..19799)
                     /locus_tag="ACHGNS_001006"
     CDS             complement(18339..19799)
                     /locus_tag="ACHGNS_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6936968.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(19789..21084)
                     /locus_tag="ACHGNS_001007"
     CDS             complement(19789..21084)
                     /locus_tag="ACHGNS_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFN6936969.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(21416..22093)
                     /locus_tag="ACHGNS_001008"
     CDS             complement(21416..22093)
                     /locus_tag="ACHGNS_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFN6936970.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(22123..23214)
                     /locus_tag="ACHGNS_001009"
     CDS             complement(22123..23214)
                     /locus_tag="ACHGNS_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFN6936971.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(23315..23749)
                     /locus_tag="ACHGNS_001010"
     CDS             complement(23315..23749)
                     /locus_tag="ACHGNS_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFN6936972.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(24333..25790)
                     /locus_tag="ACHGNS_001011"
     CDS             complement(24333..25790)
                     /locus_tag="ACHGNS_001011"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN6936973.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(25787..27682)
                     /locus_tag="ACHGNS_001012"
     CDS             complement(25787..27682)
                     /locus_tag="ACHGNS_001012"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFN6936974.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(27740..29515)
                     /locus_tag="ACHGNS_001013"
     CDS             complement(27740..29515)
                     /locus_tag="ACHGNS_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFN6936975.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(29565..31061)
                     /locus_tag="ACHGNS_001014"
     CDS             complement(29565..31061)
                     /locus_tag="ACHGNS_001014"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN6936976.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(31255..32877)
                     /locus_tag="ACHGNS_001015"
     CDS             complement(31255..32877)
                     /locus_tag="ACHGNS_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFN6936977.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(33205..34518)
                     /locus_tag="ACHGNS_001016"
     CDS             complement(33205..34518)
                     /locus_tag="ACHGNS_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFN6936978.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(34763..34987)
                     /locus_tag="ACHGNS_001017"
     CDS             complement(34763..34987)
                     /locus_tag="ACHGNS_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6936979.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(35007..35429)
                     /locus_tag="ACHGNS_001018"
     CDS             complement(35007..35429)
                     /locus_tag="ACHGNS_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN6936980.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(35571..36281)
                     /locus_tag="ACHGNS_001019"
     CDS             complement(35571..36281)
                     /locus_tag="ACHGNS_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFN6936981.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(36299..36796)
                     /locus_tag="ACHGNS_001020"
     CDS             complement(36299..36796)
                     /locus_tag="ACHGNS_001020"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6936982.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(36833..37285)
                     /locus_tag="ACHGNS_001021"
     CDS             complement(36833..37285)
                     /locus_tag="ACHGNS_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFN6936983.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(37420..38025)
                     /locus_tag="ACHGNS_001022"
     CDS             complement(37420..38025)
                     /locus_tag="ACHGNS_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFN6936984.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(38475..39095)
                     /locus_tag="ACHGNS_001023"
     CDS             complement(38475..39095)
                     /locus_tag="ACHGNS_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN6936985.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(39097..39966)
                     /locus_tag="ACHGNS_001024"
     CDS             complement(39097..39966)
                     /locus_tag="ACHGNS_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFN6936986.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(39989..40396)
                     /locus_tag="ACHGNS_001025"
     CDS             complement(39989..40396)
                     /locus_tag="ACHGNS_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFN6936987.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(40415..>41703)
                     /locus_tag="ACHGNS_001026"
     CDS             complement(40415..>41703)
                     /locus_tag="ACHGNS_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFN6936988.1"
                     /translation="GVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKTL
                     LGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALTF
                     LAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGACF
                     AIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLPT
                     HFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVYV
                     LLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTIIA
                     MFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALLV
                     TIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT        11759 a         8983 c         7021 g        13940 t
ORIGIN      
        1 ttttattgaa cttaaggttg attgatcaca tctaaaagcg ttgaaaataa aatggcacta
       61 gaagcagctc ctgggtcgat atgtccgatt gcacgctctc ctagatagga agctcgtcct
      121 tttttagcta ccaagacttt ggtgtgttct tttgcctggt caatgatttc ttttgttaaa
      181 ttccctgcct tcaatgcctc tgtgactggc agccaaacat ctaacatcgt tttatcgtca
      241 gcttctgcct ttcctcggct ttgaataccc gctgttccct gctcgatcac ttcgcctaat
      301 ttttcttgag aatcaataga atcgacctct tttgtcgctt tcatcatgtt catgaatgca
      361 gatccgtata acggaccaga agccccaccg actttcgata tcatcgccat agagaatact
      421 ttgaatgtat cgctgattgt ttcaggatga tcggcttgaa aagcttcttc ataagctgaa
      481 acccctcttc ccatgttatt tccatggtct ccatcgccaa tcggggtatc tagatcactg
      541 agatattgtt tgttctgatt gattgcctct gcaaattgcg tgagccattc ttggatttct
      601 tgaacggtta gtttcatatt atcacccttt tatcttgaga ttttttacca tgaaatcgtc
      661 tgtacattgc tttctaaaga atctttccat tgtgtctccg tcaagtcgat cagtgttaga
      721 gaaagacctt gcatatctag tgaggtcata aaattaccga cttttcggaa aactacattg
      781 acgcctcttt cttccaacag agctaaaaca tcattcatga acacgaactg ttccatcaaa
      841 ggcgtcccgc ccattccgtt gactagtaca cctgcttctt ttggtaaatt cgagtaatca
      901 tctaaaatct tagtaacaag ttcctttgcc agtactttag aaggctgcat cttttctcga
      961 cgatagcctg gctcaccgtg gataccaaca ccatactcga tctcatcttc tcctagttcg
     1021 aatcctggtt tccctacttc tggtacagtt gctgctcgaa gcgctagacc gatcgtctta
     1081 gttgcctgta cgaccttttc tcccagttct ttcaactcag ctagggaagc accgtttctt
     1141 gctgcatcgc ccacgatttt gtgtacaagg acagtacctg caactccgcg ttttccagca
     1201 gtatacgtac tattttcaac ggcgatatca tcatctacga tcacactttc tacttcaata
     1261 tcgtccatag cagctaattc ctttgccata tcgaagttca atgcatcccc tgtataattc
     1321 ttaacgatca gcaaaactcc ttttccacta tcggcttcct taatagccgt ctgaatctga
     1381 tctggagttg gagaagtaaa cacatcacca agcaccgctg cacttaacat cccttcccct
     1441 acaaacccgg catgggatgg ttcatgtccg cttcctccac cagaaaccaa accaacttgt
     1501 tccgctttct gattcttggc tacaacacgg gaagattcta cacgatgaac aagttctgga
     1561 tagcttttta ctaatccttc cagcatttct tcgacaatat tgcttggatc attcatgatt
     1621 tttttcaatt tattcccatc ctttctttcc ggcattttgt gcttctgtta agatttgctg
     1681 agtatcatct gttataccgg cagtgatggc tgctgcaaaa gctccttcca ctaaaggcgc
     1741 atccaccaga tgataacgtt gctgctgctc ttcttccaac atgtcttttg ccagttccga
     1801 gttcaatacc gcgctaccga tatccgcaaa aacaaaataa ctagctgcat cgcttgattc
     1861 attgactgct tctacgattt tcatcgggtc tgatccgatc tcgccattta ttccgccgag
     1921 agaaaaaatt tgcacctctt cggatttttg catctgctca atcatttctt taataccatc
     1981 tgttactttt tgactatgag aaataagcaa aatacttttt ttcacaagaa tcttcctttc
     2041 ttttacatag ttgctaacga ttggtttcgc tttcattaat gaacaattca agtatataat
     2101 aaatttctaa ttttgggaaa atatgtgtag tgcttcttga agtttgttat actttaatta
     2161 aaagagagga gtgatcgaat gattacaaca acaacgaaca gtgtagaggg aaaagccgta
     2221 gtagcttata aagggatcgt ttttggtgag gtcattactg ggatccatgc atttaaagac
     2281 ttggaggcgg ggttgcgaaa tatctttggc ggccgctcaa aaagctatga aaacgaacta
     2341 atgggtgcaa gagaagaagc acttgccgaa atggccgaac gtgcaaaagc attaggagct
     2401 gatgcgatca ttggcgtaaa aatggactat gaagtattag gcagcgacaa tggcatgctg
     2461 atggtcacat gtagtggaac agctgtctcc acgagataag taggggacat ctgttttaat
     2521 aactaaagag agatgggaca tgtagtggca ctcaagaatt agatttttca gtctaacttc
     2581 gaggtactct accttcccat cctctctttt ttatttcttt ttgcggatga ttccgtaagc
     2641 tgctgcgact aacacacccg cccctgctaa ggaataactg acagaagctg ttttttcatt
     2701 tgtttttggc aaagtctgac ttgttgattt ggatggctct gtcgtttgct gaactgtttc
     2761 tgtcgtgctt ccagaggtca ctgatttttc tcctgccgaa tgcaaaatat cttttattcg
     2821 ttggaaacta gctgctaagt cagctttttt tgcttcaaaa tcattagcaa ctgtttgtgc
     2881 ttcgctgatc tttccgtgga ttgcttgact ggccgctgtg atatccgctt gagtttgact
     2941 tagctttcca gaagctgttt gagcaacctg atttaattgc tgattgaatt cttttgcttg
     3001 ttggatcttc ccgtcaagat ctgtttggac agcttccgct tttgctttcg cttcggcaag
     3061 ctttttctca agttgtgcac ggtcatttcc cactttattt ttaagatcag ctaaatcttg
     3121 atcgattgct gcttggtctt ctttcaattt gttttttgca gcttctaatg cttcttcgag
     3181 ttcttttctt ttattttcca agtcttgcaa atgatcgatt tggtcagaag aggtagaact
     3241 atttattgat gtatcttctg aagatgaaat cgtttctcct gaattatctg tcggtacatt
     3301 ggaagtcatt tctgtttccg aggcaccgtt ttcagcacca gaagagctta tggttgttga
     3361 aggttctgtt tgagcaggtt cagaagtttc cgcaaaagct gtctgtccca ataagaaaat
     3421 actggaaaca gcaattggcc agattaacgg cttcattctt tttttcatca cttttcccat
     3481 ccttactact ttttaactaa tttcttatta taagcaaaaa tgtttggaag aaccataaac
     3541 acaacaggct gtttgtctaa aaaacggtgc atcttttatt tttttgtacg ttccgggctt
     3601 ttccatgtcc cgcttccata acctaaaatc gttttgactg ctggaatgaa agtcgtcacg
     3661 atcgttatcg cgttcactat ccagaacaaa agcatatata gtggtgcgaa aagaaggtat
     3721 ttcaattttc ttcgtcgatc atctacaatc agcgaagcta gcaactggaa aaatcctgcg
     3781 accatttcaa agcagatgaa cacaaaactg aatgtaaaag taatataaat ctgttcgtag
     3841 tttccttgat atacataatg cccgattaat ccagcaaaaa gaactacgga aatacaaaag
     3901 aatattgacc agacaatact caatgtttga tcaatgaaga tgatcgttct tccaatatgt
     3961 ttaaaaggat ggagcatcac ttttttgaaa ttcgtcagcc agacttctgt tccacctttt
     4021 gcccaacgtt ttctgtgtcg atatagcatc ttcaacgtca caggtacttc catgaaaaag
     4081 ataatctcag gagcaaaaac agataaccag tcatttaact gatgatccca agcaatactg
     4141 atgtcttctg tcgctcgatc ttgtctgaat ccgcccacat cgatcaaggc ttcttttcga
     4201 tacatcgtat ttgctccact gtacgcataa agaccaccga aaacagctga ctgtgttcgt
     4261 ttaatgatgc ccacgatact tgagaattca accgtttgag attttgcaat caatttggta
     4321 cgattttgga catccatatt tgcagtgacc gcagcaatat gtcttgcacc agggcgaatg
     4381 aaataattca catacctaat cagtgcatct ggctccggta ccgtgtctgc atcattgctt
     4441 aaaatcaatt tcccttttgc aaaggcaagt ccgatattaa acgcgtgggc ttttccttta
     4501 tttttttcga tccgaaccac acgaagatta gcgtattttt tcatcagtct agctaatatt
     4561 tctggtgttt ggtccgttga accatcgtct gtcactaata cctcatagtt gtgataattc
     4621 aatttcgtca tcaaatactc gattgtatat tcgatcacaa tttcttcatt atgggctggc
     4681 accatgatcg tgatgaacgg ttcgacactc aagggaacat ctacccattc tttttgctta
     4741 tgtttgtata aaaacacata acaccagaca ccaataaacc atacaaatcc gcctaaaatc
     4801 ggataagaaa cgagaaaaaa atacataata gacaaacaag tatctaagaa taaattacct
     4861 gtatgggtga ttgagatcat ttcagttcca ccccattttc tttatacaaa tctgcaatga
     4921 atgtcgtatc tagatttttc tcttcggaga cagaataaaa acggaccgtt tctctctctt
     4981 cttttgttcc aaaacgttcc gtatacactt cttcaagtaa ttcttgtctt tttttcaatt
     5041 tttcatcttc atattcaaaa gatttattta cttttttgta tagatttcga ttgttccacc
     5101 tagtcaatac aaatgaggta atcaggaaac tgaaaaaaat caatacaaaa aagccgatta
     5161 agaaatacag cattttcttt tctgataaaa aagcgtacaa ggaaaaatcc ttggctcttt
     5221 ctggtctcgt taatgggaaa aagacccata taaaaggaag aacaacgatc atccatccta
     5281 aaagagccac taacgtttgc caaatcttta accagtaatt accttttgaa aaatacttat
     5341 ctttcaccaa aattgatgca acttcttctc ttgttacttt tttcatatcc tcacctcacg
     5401 tttttttgtt acgacgtagg agtttaccat acatttcatt tcttttcaca taaagtgttc
     5461 ctaaattttt cctaagaaaa agaacaaaac atttctcatc agcaaatgaa aaatattttg
     5521 ttctttttat cagttgttct ttttattttt tatctcttaa ttatgttgct ctttccaata
     5581 cgacaatgct tttcattcct ctagatatcg ccgtgtacat ttgtctgcgt cttctttgct
     5641 catctccatc gttttcaaat ggatataaga ttacatgatc aaattccagt ccttttgcta
     5701 gactcaaagg aatgatttgg cagttttcta atccatatga atttttttta atcgattctt
     5761 gtagcgtttg ggtttcctct tctgtttggg tgataactgc cgctttttct ccttttagag
     5821 aaaccaaaat ctcagctagt attttcaaca attcttcaga ggatcgaaaa gagcggtatt
     5881 cgggttcttt tccttgcggt cggatcgata cgatgtcgat cttctcttga tccacagcat
     5941 aggttttgaa aagctctgtg attgctccgc tagaacggta actatttcta agatcatacc
     6001 gatggatcgg cagatggtag tcgtcgaaac aacgcataat atcagcaaaa gtagtggaag
     6061 aattaaagat tgcctgattc tcgtcaccta ctaaagtaaa gtgagttttt ggaaataatt
     6121 cgatcaatag acctaattgt gccagggtat aatcttgtac ttcatcaatc aatagatagc
     6181 gaaatgctgg tacatttacc ttttcaacaa gtaaatgacg aatagctaat ataatgactg
     6241 cttcatcaac agtacgtgtg ggttgccaag cataagctga gccatatcgt ttttcataaa
     6301 ttctttcaaa caactgatgc tcatccaccc atgccatttc ttcgatttgt ctggttattt
     6361 ttcgatattt tttttgtaaa agtttccttg cataagctac aatactttgt tccgaatcat
     6421 cagaaatcaa tttaccgaat aattccaact gcctatcttc tgtcaacgag agtacctgat
     6481 tctgcacagc aggacttttt gcttgtttca aaagatgatt cttccattcg ctcatcagtg
     6541 cctttttcgt tgcttgaagc ctatcgatca atttaggatg agcaggaact ttctcatata
     6601 ttttttcgat cgttttttta gaaaggactg tttttccatt tttagtaagt ccacggataa
     6661 aattcggatg atctaaaaac aaaggatctg attgttttag attatcgata aactttttgc
     6721 tacgcaaacg ttctgtctgc tccgatacat tttctcccgt gatacgtttg aaataagcct
     6781 cttcaccttc tatttcctct gctgaaagct gtgaaaccag ctgtatgatg gttagattca
     6841 gtggatttct ttcacctaat gatggtagga cattcgaaat atagtcgata aaccgatgat
     6901 taggtgatag aatcagcaag tcatcggatg tcatcgtatc tcgatataaa tagagcagat
     6961 acgcaatacg ctgcatgata gcagaggttt taccacttcc tgctacgcca tttaccaaga
     7021 tattttgact tttcacatcc cgaatgattg tgttttgttc cgattggatc gtagcagtga
     7081 tagccttcat ttcattggtc tcatcttggc ctaagacttc taataatacg tcatcttgaa
     7141 tagctacaga cgtatcaaaa aacttgacca aacgatctct ttccaaaaga aattgtcgtc
     7201 tttctttaat aaccgtatcg attttatttt gacgaaccgt ataagaagat ggaccaatca
     7261 tgttattgta aaaaagttcg gcaattggtg aacgccaatc ataaacaaga ttttcatctg
     7321 catcatctgt gaaatttgct gtaccgatat aaaaggattc ttttggctca ttatccagaa
     7381 agtctacaac gacttttcca aagtagggac ttttcaataa acgttcgatc tttgctagct
     7441 gattttctgc gtttttctgt ttgatattca tctgatcgat ttctctattt ttcatttcaa
     7501 tcatcgaaaa agtatccagg ttatctaagt aactgtcaaa attcaatcga agctcgccag
     7561 ccatttcttt caacgactgg tttccttcta tttttgccgt atccatcgat tcccgcagtt
     7621 cgtttttttt atcaattagc ttttgataca cgttcttcag atatgcttct tctaactgtt
     7681 tttgtgtgtt gaccatacac attctcctct ttttttaaag tggtggaacc gattagttta
     7741 cctcttttaa gagtcgaatg caagcttttt aacgagtatc atttgttagg atatatcagt
     7801 tcttcaggtc ggacagtttg atataaagaa aatgcaccat caaggttttt tacgtggtag
     7861 ccagactgct tcaagattct ttccgctaga tagctacgta atccgctatg acagctgacg
     7921 atatattctt gttggctgtc taattcattt aatcggtccc gaagttcatt caaagggata
     7981 tgtttggctt ccttgaattt gcccttttcc aattcttctg ctgttcgcac atctaataaa
     8041 atttttcctt ttgctagttc tgtcggcaat tcatgccatt gtacggtttc actaagccct
     8101 tctgcaatgt tcattgctgc atatcctagc atgtttactg gatctttggc tgagccaaat
     8161 ggcggtgcat acgtcaattc taattccggt aaatcgaaga tcgtcaaatg ccctttgatt
     8221 gctgttgcta aaatatcgat ccgtttatcc acaccttttg ccccaacacc ttgtgcacca
     8281 taaatctcgc cagtctctgg atggaagatc aacttcagta aaatatctgt tgcttctgga
     8341 taatagcttg catgatcttt tcctgataca tgaatgactt caaaagacag ttcgttttct
     8401 ttggctgttc tttcacttaa accggtggat gcggcagtca ttccaaacgt tcgtataatt
     8461 gctgttccaa taccgccttg atttcttcga gaaattccgg aaatcgtgtc cgcaacttgt
     8521 ctaccttgac gattggctgg tgaagcaaga gaaataagtg catcttttcc agtgatttgc
     8581 tgttttacaa cgatagcatc ccccaccgca taaatatcag gaatattcgt ttgataacgt
     8641 tcgtctacta ggataccccc gcgtatgcct aattcgattc cagcggtcac tgctagctgg
     8701 ttatcaggac gaacaccaat ggataaaata actaaatcag cagggatggt acgcccatca
     8761 tccagtcgca atcgtttccc gtggtcttca aatccagcga cagcatgaga agtaatcact
     8821 tctactcctt gatgagacaa ttctttttct ataaaagctg ccatttcttc atccagtgtt
     8881 ggaagaatat gtggtgcttg ttcgatcacc atgacttcta aacctcttct ttttaggttt
     8941 tctgccattt ccaatccgat gaatcctgca ccaacgatca cggcacgctt tggctgtttt
     9001 tctaatgcat gcatcacttt atcgatatct ggtacatttc taatagaaaa aacattgtcg
     9061 gcttctgtta tccctggaat cgatggaaca actggttttg caccaggaga taaaaccaat
     9121 gcatcgtact gttcaatgtg tttttgaccc tcatgtacga cttctaccgt tttgttttcg
     9181 ggaaagatgg ctgtcacttc atgatttgga cgcacatcta aacaaaaacg agcggataat
     9241 gattctggcg tttggacaag aagattttcc cgttcactga tttctcccga aagataataa
     9301 ggcaagccgc aatttgcaaa agaaacatac ggtccttttt caaaaacaac gatttctgca
     9361 tcttccatta atcggcgaag ccgtgtagca gcagacattc cacctgctac acctccgaca
     9421 ataacgattt tcatcttatt tccctcctac tgttcgatca aaccattgat tcatgccacc
     9481 acgtacattc actacatcat aaccagattt ctttaattct ttagctgctt gtttactcct
     9541 catccctgat tgacaaatca catagacggt cttttctttg tctccatcat atctgtttat
     9601 actttgtaat ggaacatttt tagctccttt tatatgcccg ccccggtatt ctgcaggtgt
     9661 tctaacatca agtaacgctg gattttccaa aagcttttcc tttaattctg ggactgttat
     9721 cgattttatt gattgaaata gcaaattcat tttctgcacc tcatttacat ttttataaat
     9781 acctcatggg gtatattaaa caggagcttt attaatatgt caaccagaat gactcttcat
     9841 ttttttcact actttttata tttgcataaa ataccgataa ggggtattat ctaattataa
     9901 aaatagagga gaatgtgtta tggagattga taataaaaat atcctgaacc gtttgaaacg
     9961 tacagaggga caaattcgtg gtatccaaaa aatgatcgaa gacgaaaaag aatgtatgga
    10021 tgtcatcact caattaagcg ctgttcgttc tagtattgat cgcgtaatgg gcatgatcgt
    10081 tgcagataat ctgaaaaatt gctttgagaa acctgatagt aatccagaag aacaagcggc
    10141 taaactagaa caagcgatca agatgattat taaaaagtaa aggaatgatt tcaatgaaag
    10201 tcgttttcca tattgatgaa ttagaaaaat ggtcagaaac agggaaaaac gtaaaaaacc
    10261 tgataaaagc ttctccagaa acgacgattg tcgtatctgt aaatggcatc gcgattaccg
    10321 gttatctaga ctctgccaat gcagaattct tagatttgca aggagtcgtt tttcacgctt
    10381 gcgccaatgc tatgcgtgct aatcatattt cagaaagttc tttacctgaa caagtaatcg
    10441 ttgtaccagc aggtgtcctt gatttagttg aactgcaatc ccaaggttat gcttatatca
    10501 agccttagtc agtagaggtt gcgacagaag tgatcagctt caagaaattt cggcttattt
    10561 ttcgaagaag ctacttctgg aaaaccgtga atcaaaggtt gtgaaaacgc tgtcctgcat
    10621 caagtaataa gaacagccaa acaaaaatag cttctcatat ttttcgtttg gttgggctta
    10681 ttaccgcaga tgcaagcttt tgaacaccgt ttaaggaaga ggttgtgaaa tcagcggttt
    10741 gacctaagta ttagagaaaa aaacgggaaa atagctcctc atattttgcc gttttttgag
    10801 ctaataccga aggtcagctg attgaacacc gtttaaggaa gagattgtga aaaagtcgct
    10861 tagtttcgag tattaagaaa aattttgtga aaatggattt ctacattttt gcataaattt
    10921 ggcttaatac cgagaaactg gcttttgaat accgtttaag gaagaggttg taaaatcagc
    10981 ggtttgacct gagtattaga gaaacaaacg gaaaaatagc tgctcatatt ttgccgtttt
    11041 ttgagctaat accgaaggtc agctgattga acaccgttta ccaagaggtt gtgagcctac
    11101 catttaactc cgaatcacaa gaaataattt caaaaacaac ttctcctgtt tttggttcgg
    11161 ttggcttatt atcgagaagt agctactggg agtctattta tcaaaaaaag gaatgcgaca
    11221 taggtcacac ccctttttta ttttctttga aacatctttc ggtactcgac agcatacaag
    11281 acaaaagatt gtaaaagacc gtaagtgatg acggcaaaaa tgactctcac ccacagcgtt
    11341 ggtaattctg ggaaaagcag caaaaggatc atgccaacat agatgacagc agtcgctact
    11401 ttgccatacc acttcgctcc acttaatttg atatttttag atagcccgat taatccagct
    11461 acaaacataa agccttcttt tatcaagaat agaccaaaca acagccaaag ccacggtcga
    11521 taccatgcca tagacaaaat cagaaaaagt tgtgtcaatt tatctgcaaa cggatcaagc
    11581 accttcccta agtctgttac ctgattatat ctccgagcaa tgaccccgtc tagataatca
    11641 gacaaacctg agacaacaat gaccgctcct gctatataaa atgactgtct cagcacaaaa
    11701 taaagataaa tcggcagcaa aaaaatacgg aatatcgata aaagattcgg aatcgtttgt
    11761 atttctttac gccagtctgt gcttaatttc attttatcgc cctacctcct cagcactctt
    11821 ttctttttat tttagcttac acggggaata aaaccaatga tttcactgtt tttatttttc
    11881 tttacagaca ctgatattat gagctaattt gagataatca cttgctgttt tcatcgttta
    11941 ctcttctttc ttcctgtcgt ttgcgcattt cttttactag tactttatga gctttttttg
    12001 atcctcgctc taggtcacgc tgccgtttac gtgttttata atcgataaac agactgtcta
    12061 aatcgtaacc tgtcgggtac agatcttctg ctgaacgcac cagacgaaca cgctgacgag
    12121 gcacgtggat tatttttttg tctaaatata catcaatcgt atcttcacct atatcttcat
    12181 aaactaacgc ttctttttgg tattcattga caaatatccg atcacctttt gcaaagagct
    12241 gacgttcttt tttattctcc ttcctatttg taactgctgt cttaggaaga aattcttttt
    12301 tctgtgtccg ataggctttt tccgccaagt aacgctctgc ttgatggatc aatttcatcg
    12361 acatatccat tttatgagca atccacaaag cttggctttc tcctgtttct tccaacccgc
    12421 aactgatatt taggacgaag tgcttcccga tcgaaagcca tagcagccgg cacaaaatct
    12481 tcatgatctc cggcgaattt cttgatttct ccataatggg tcgtggcaat gatcaatgcc
    12541 ccttgctcat acatagattc catgattgcg atcgctagtg cagccccttc atttggctct
    12601 gttccactgc caatctcatc taaaagtacc aacgtattcc tttttatctt tttcaaaata
    12661 gcagcgatgt tttgcatatg accagaaaat gtactcaacg cattttctaa attttggtgg
    12721 tcaccgacat cgacgaaaat ctcatcaaag acagcaagct ctgtcccttc ttttgccgga
    12781 acttgcatac cgaacatcgc catcaaagtc aataaaccga ctgtcttcaa cacgactgtt
    12841 ttgcctccgg catttgctcc ggtgataatc aagcctcgat agtctttacc gatttcaaca
    12901 tccaatggta ccgcatgctc aggtaaaaat ggatgtctgc cttgcttgat aatgatatgt
    12961 tccgatttat tgattctagg tgtgcttcca ttgatttctc ggctaaattt tgcccgagca
    13021 aagatgatat ctaacaccgt gatcgtttcg atcaataaat cgatcacttc ttcgttttct
    13081 gcgatagcac cagtcaatgc cgccaggact tgatattctt cactgatttc ttcagctttt
    13141 aacaactgat agtgttcatt caatttagaa atcacagttg gttcaataaa aaccgtcgtt
    13201 cttttatttg attcatcgat aatcgttcca tctattttat ttttatagct ggctttgata
    13261 ggaatcgtat agtattcgcc tttttgaaca atcattgctt cttggatcat ttctttgttt
    13321 ttcggatgac gcaagaattt cagtaatttt gactggattt ctttttcgat tagttgtaat
    13381 tgtttgcgta ctttacgcaa attacgtgac gcgtcgtcac tgactttatt attttttatt
    13441 ttctggtcaa tcaactcttc tacatttatc aaatcaggaa gatgtttgct ataagcaaac
    13501 aacaagggtg tctggtattg attcttatca aaaaactttg tgatcatccg actgcttcta
    13561 agaaaatccg catattcgat caaatcggtt ggctgcagaa tcaacccttt tttgacttgt
    13621 tctgtcagtg catcgatcct cggcaacccc ataaatggaa catgctgact gctttctaag
    13681 atcaagcgtg cttctcttgt ttcttcttga cgatccaaga cagtttgtag gtttgtcgag
    13741 accgtcatat cgctgattcg ttttttgcta tagtttccaa ttgctcttgc ttggatttcc
    13801 tttttgatcc gatcaaattg taattgacta atcatttcgt tgtccatatt ctctcctttt
    13861 cttgaagaga tacctgtccc aaaataaata agggcctaga gataaataaa aggatagaac
    13921 acagcttaaa aaagtcatgt cctatcctta ttagaataca aagatattgt tttcctgaca
    13981 ggtaccgttt cataagcaca ccaaataaac tttgtttatt ggctattcga cttactgatt
    14041 agataccagc tacactcaca aaacataccc cattcttcta ggtcttattt attttccact
    14101 taactatagc aagacacatt ttggttgtca aactttagta tgttttatag atttacacca
    14161 ttaatgaata gacacctacc gcgataattg ctccgatcat tggacctgcg attggtaccc
    14221 atgaatatgc ccaatctgaa gttcctttat tagctattgg caataccgca tgtgctaatc
    14281 gaggaccaaa gtcccgagca gggttgattg catatcctgt tggccctcct aatgaaagac
    14341 cgatggctaa gatcaatcca cctacagcga atacgttcgt tccaggtgca aattcgtttt
    14401 gtccaaaagc tagcaaccca agaactaata caaatgtacc aattaattct gtgatcacat
    14461 ttgctggata attccgtata gcaggacctg ttgcaaacgt tcctaaaatc gcgctttcat
    14521 ctttcgttgc attccaatgt ggcaaataag aaagccaaac aagaatggcc cctgcaaaag
    14581 ctcctaaaac ttgcgcaatg atgaaaggaa cgacaagatt ccaactaatt gcccctgtca
    14641 tcgccatagc tagagagact gcaggattca aatgagctgg gcccatgaat ccagaaatat
    14701 agacagccat tgttacagcg agtccccaac caaatgcgat gacaatccag ccagaagcct
    14761 gtgctttact tttgtttaaa ttcacagccg cacacacacc atctccaagc aaaactagga
    14821 tcagtgtccc aataaactcc cccatgattt gagtcatatc actgttcatt tatttctccc
    14881 cctttagttc ccgcagatct gattctgcaa tcaatgcttc taaacgttta gtctgttgcg
    14941 ccttctcctc ttctgtccat tcaaaatatc cagcaattgc atcgatcaca ggttgcttga
    15001 tctcatcaag ctgatctcgt tcaaacaaca gatgatttgt acgacgaatg agataatcgc
    15061 ctggggccaa aaccatttct tcttctaaac cataacgtaa ccgagctgac tcagcgagac
    15121 tcaaaccagg ataaggtgcc atttcttttg ctaattcaaa gatacgtcgg gcattagtcc
    15181 cgtaaaaatc agcgatataa gtagcatctt cttcctctaa accggctgct actccttctt
    15241 ttgctaattc tgtcactgtt tcttctaatt tagttggatc gaagtttcca cctgaaatct
    15301 gatatttttt agagtcgatt tctttcgtct ctattccgta ttcttctttt aataattggc
    15361 gtatcaatcg caaagctcct tctgccatct tacggtaatc ggtgattttc ccaccagata
    15421 aagtcagcaa accatctggt tctctttcta aagaactacc tctggaaatc gtagaaggtg
    15481 ctttttcatc acggctgttt tccaaatggt tcaacacgtc ttctacttca gcacgagaaa
    15541 ctttgttttc cttatattca cttactgtat cgacaacttt attgaagctc ttgtctgaaa
    15601 tcgatccatt atctccacca ttgtaatcag aaccagaatt accaatcaac agtggacgaa
    15661 gtcctgccca gcttgcttcg atatcattca atgtgatatt tgcttctgga tagcgatgat
    15721 tgatcacatc caatagataa tctacatctt cttgtgtgac tttcggatcc gtaaaatctc
    15781 cttggtaatc cgtatctgtc gtaccaaagt aagtcttgtt ttctcttgga atagcaaaaa
    15841 ccatccgccc atcttgcttt cctgtatcga agtatgtggg ttgcggtaca ggcagttttt
    15901 tcgcatctac aactaaatgg atccctttag ttggacgcat tttaggagag actggacgcg
    15961 taaaattcaa gtttcgtact ttatctaccc aaggaccact cgtattgatc actaattttg
    16021 ctttgatttc gatcacttca tctgtcagca ggtcacgagc tttgacgcca acgatttgat
    16081 cgccatcata taaaaagcca accgctttca ttttacttac tagataagcc ccatcttcgg
    16141 cagccttttt gatattatca atcactaaac gggcatcatt gttgcggaaa tctagataga
    16201 cacccgcgcc ttttagccct tcttttttca aaaacggttc tctttccaat acttcttctg
    16261 gtgtcagcgt ataattctca tatttggttc ctgtcacatt tgccagtttg tcataaaggt
    16321 ccattgctac tttgaccgag aacatattga aggttgttgc tccttcgccc tcatagattg
    16381 gcaaaagcat tggatctggt tttgggatgt gtggggcaat actttgtacg actgcacgtt
    16441 caccaactgt gtcagctact acttccacat caaatgtctt cagataacga ataccgccat
    16501 gaacaagttt ggtcgagcga gaggaagtcc cttctgcaaa atcctgcatt tcgatcaatc
    16561 ctgttttgat tcctgatgct gctgactgga ctgccacacc ggcaccagtg attccgccac
    16621 cgatgatcaa cagatccaat tcttcttgct gcattttttg aatgctatct tgtcttgtct
    16681 tgtttgaaaa catatggatt tcctccttat tcaaatgctt gggtagcttt tactgctttt
    16741 ttccaaccgt tgtaaagttt ttctcggcgc tcttcctcca tttgaacttc aaatagcttg
    16801 ccagcttcat aaaattcgcg gatctcgtct gtatctttcc aataaccgac agctaatcct
    16861 gctaaaaatg ctgcccctag cgccgttgtt tccaagtttt ctgctcgttg gatcggtaca
    16921 ttcaagatat ctgtttggaa ttgcatcaga tattcattgt ttgctgcacc gccatctact
    16981 ttcaatacag gaatcttgat tccagtgtca tcttgcatcg tatcgatgat atctctcact
    17041 tgataagcaa tcgactgtaa agtagcttta atgatatctt cttttgtcgt accacgtgtc
    17101 aagccaaaca tagcccctcg tgctgcctga tcccaataag gagcacctag cccaacgaat
    17161 gcaggaacaa cataaacctc atcttcgctt gtagagcgtt tcgctgcgtc ttctgaatcg
    17221 ctagcttttt gcaacatttg aagtccatct ctcagccatt gaatcgaaga accagccaca
    17281 aaaatacttc cttctaaagc atagtaaacc tttccattta tgccgtaacc aattgtcgtc
    17341 aataaattat ttttggataa ttgcggtttt tcaccggtat tcatgacaat aaatgaacct
    17401 gtaccataag tattcttgac catgcctggt tcgaatgcca tttgtccgaa taatgcggct
    17461 tgctggtctc ctgccatccc tgaaattggt gcttcgcttc cataaaaatg ataaccttgc
    17521 gtataaccat aaacttctga gttagaagtg acttttggta acatcactcg aggaatattt
    17581 agtaaatcta aaatctcctg atcccaatct agctgatgga tattgaacaa catcgtccga
    17641 ctggcattcg agtaatcagt aacatgtgct tgtccatcgg tcagtttcca tactaaccaa
    17701 gaatcgatcg ttccaaacat caattcgcct ttttcagctc gttcttgcgc gccttctaca
    17761 tgatcaagaa tccagcgtat cttcgttgct gagaaatagg catcgatcac taaccctgtt
    17821 ttttcgtgga aaaattcttg atagccttca ttttttaagt tatctgcaat tccagctgac
    17881 tgtctggatt gccaaacaac tgcatgatag attggacgtc ctgtatcttt ttcccatatt
    17941 actgttgttt cacgctggtt cgttatgcca atcccagcaa tctgatttgg tttgatccct
    18001 gattcgatga acgctccggc aataactgac tgtacagaat tccagatttc atttgcatca
    18061 tgttccaccc aactttcttg cgggaaatat tgcgtgaact ctttttgaga actgccgatg
    18121 tgtcttcctt tcttgtcaaa gataattgct cgtgagctag ttgtaccttg gtcaatcgcc
    18181 atgatatacg ttgattctgt cattgtgatt ccctccatta tatgcaaacg ctttctttaa
    18241 ctactcttat catactactg ttttaaaaaa agaaactgtc aaaattttat tctttttttc
    18301 tttttttgac agttttttat tatgaagcta ttttttaatc aattcttttc ttttttcagc
    18361 gtctctattc ttcttttcaa tcgaataatg tcgtaaggtg acgcaaattc tgacagataa
    18421 tattcacact gttttttgtt tctctggcaa aatgtgatca ccaaatcata ttctttacct
    18481 tcttcataac ttccaatctc aataccgccg attcccctca gttctctgat cagatattgt
    18541 tgaaaagcaa tacgataaat cggcagactt tctaaatcga ttccaatcac tactggtttt
    18601 gcaatataaa aatcgatcat caaaagaaca ttggcatact ggatcatcag gtaactaagc
    18661 tcactatctt cagctctttt cctcggcaat tgctgcaccg ctgtttcctg taatttcgcc
    18721 agtaactgtg ttaaggaatg cccgagcatt ttctgctgct gttccaaaag ccgatcacgc
    18781 tcatagactt cgatctttcc aacaaagaaa aaccattcat tgttgatctg ggaaatttgg
    18841 tagcctactg ccttttcttc tttgattttc aaacgacgag gacgataatg ccataacatc
    18901 gtttcccgaa tgtaagtatc taaaacagcg gttggtaact ttttgctccg tgtcaaatca
    18961 tactgataaa atatttcttc gtctactatc gaaaaagaaa caaaaaagct gtaaaacatc
    19021 attgtttcgt atacattcag ttctgctgct gttcgactaa gatgcatcac gatgattgga
    19081 tcgattgctt gataaagccg gtccgactga tagagtaatt tcttttcttt caaagttgcg
    19141 tacgtacttt tttcattcaa tagtcttttt ttcgtaatcc cgagccagca agcaatctgt
    19201 gcttctgctg atgccgtaaa cgttgtttct aatacacggt ccaatcctag aatgaacggt
    19261 cttttttctg gtgtattttg caaaaaaagc ggtcgctgat cttccgggat atattggaat
    19321 agttcataat aaaaatatcg tatctgtagc tcttctccgt ataattgacc gtttttgatt
    19381 tgtaatccaa attcctctaa gagctggttc aattctttga tctttcgaaa aacagaagac
    19441 tcactgatat taaacgctgt cgctaatcga acgatggata cttgctgatg ttctaataaa
    19501 tagacgagca tcttaaactt caagctttcc tgcaataaaa acaatagaat tttttccaaa
    19561 cttacatctt cgtccaacac aagcagcgcc tgatactcat ttcgctgtac ttctactttt
    19621 ttttccatca ttcggccaat caattcaata tcttccaaat attgatcaaa cgaggctttt
    19681 gacagttgga catgctctct caaaccattg atagaggctg ttcccccttg cagaacaagg
    19741 tttcgcaaaa tcaggatctg taactgctct tttctttcta ataatgcttc aatcttcata
    19801 ggcagtattc gccacctttg accacaccat atctggcaca cggtattctg gctggaaaaa
    19861 tagctcatgt tcttctaact gttcaaacgt ccatccttct tctactgcaa ttgtcagttg
    19921 gtcaagaagg tggaacatct cttttttgct ttggagctgt gctccaagaa tctttttttc
    19981 agaatcaaat agaaatttga gtttaacttt gctttgattt attgctgtaa gcttgtagtc
    20041 tacgtatcct gtttcaattt tttggggata aaaaatgctc tcttcttctg tcaaacctac
    20101 gcttcctaag aaatagccaa accactgatt gccgactgga cgataggttc cctgatcctt
    20161 tgtctgtgag ccactaattg tcttgctgac atttcgtgct gtacggacag cgttatttac
    20221 tagagaaaca tacatttttt catttgttgg acgaaaagtt acttggatcg catcgccaat
    20281 cgcataaacg tctttttgag acgtttgcaa gtagtcatct acccagatcg ttccatcatc
    20341 attcaatgtt aattgttctt cccacatctg attgtcggga cgagaatgat tagccagcaa
    20401 aacaagatca tagttctcct ttcttttttc cgtctctaca atagcggttt cattttcggt
    20461 caaagacaca acttgttctt ctaaaaaaag tgaaagccct tgatttttca tttcctttac
    20521 taacggttct gtcatttcag gatctagata acgaaaaaga attgactttc ggctttcgta
    20581 aagatggatt tgtttttttc ttgatgccaa cccttcagct atttcgattc ctgcttgtcc
    20641 tgcaccaata attgcaattt tctttgcatt catgatcttt tgctgaagag ccgttacgtt
    20701 ttgtatcttt ttaactgggt gaataagcgt tgattctgca gaaatatttc gaaacttttg
    20761 atttgaacca gtcgctagaa tcaaacgatc aaaatccaat ttttttcctc ctgctaaaac
    20821 gacttttttt tctttcaatc ctattactgt cttttctgtg taaacatgga ttcgatagcg
    20881 gtcagtcagt tcttctttcg tgatccaaga aagttgtttt tcactggtaa cctttccttt
    20941 taatatcaaa ttgaacccgc tgggaatcca tcctaacttc gattgctttt caatcaaata
    21001 tatttcactt gttggcatca attggcgaag ataaatagcc gcatgaatcc ctgcaaacga
    21061 cccgccgaca attacaatgt tcatctgcat tcctctttct tataaggtgt tctactgcta
    21121 acttctcaat cagaaaacac agtattcata actcgctttc aaccttatga tacatagcga
    21181 tgcagcagaa tcaaccctct ccatcatacc gtgttctagg tctgcttctc aataatgtac
    21241 tagtggacct aattgaagag tatcatttct gaagagatgt ttcaaaaaat gtactcttat
    21301 aaaagaaaaa aactcgcctt ttacgtacct ctgtccaaat aggaaacaga aaagaacgaa
    21361 aagacgagtt ttagcgtgtg taatgtaata gttagaatag acacatcatt tatttttaat
    21421 tctttaatgc ttcaacaact gcttcgccta attgatgagc agattgagga ttttgccctg
    21481 taagaagccg cccatcaatt tgaaccactt ctgaaaaagc tgctccagac tgatagtttg
    21541 ctcctttttc tttcaaagca tcttctgcta agaatggtac ttcattcgtt gtaccatttg
    21601 cttcctcttc ttcattactg aacccagtca ctgtacgtcc agctaaaata gattttccag
    21661 actcatctgt aactggcaac aatcctacaa caccgtgaca aacagctgag accagccctt
    21721 catttcgata gatttgtgaa gcgactttcc caagatctat tgcttctgga aaatcccaca
    21781 ttgttccgtg gccaccagca taataaaccg ctgcataatc atttgcatcc acatcatcag
    21841 gacgtaaggt atttcccaag gcccgatttc tgaaatcttc atcttcataa taggcccaat
    21901 cgatttcatc catattcaaa acactttgcg gttctaaagg tacatatccg cctttaggac
    21961 tgacaaaatc tgcatcgatc ccatttttta ctaatacatc ataaaaatga gtcagttcac
    22021 ttaaccacaa acctgtagga cgctcgatct tttcgtactt ttctacattt gttaatacga
    22081 tcaatacttt cattttccca tcctctttct ataaaaattg cgttattgat aacgtgcttt
    22141 caagaaatta tataatgggc ttggcaaata aagctcttta tatgcttttt caaaaggttt
    22201 cgttacaaat tcatcctcac ggttttccaa taatttctct gcccagtcag gatcgacgat
    22261 catttgctgt ccaattgctg caagttctgc atatttcaat acctgctcta catcttcgcg
    22321 attgcggatc ccaccgacac caactaaagg gatctttcct tgaatcgtgt tatggaccac
    22381 ttcaagaatc gatttatcgt tgtatttatc agaacgagct attcgatcat acatatttaa
    22441 tgaaacatgc agataatcta gttttgactc acgtaatcgt tcaagaagcc agatcgtgtc
    22501 ttcaaaacgg atacctggtg tttcatattc ttctggtgag aaacgatacc ctacgatgaa
    22561 tggtttttta gcatattgtt caaccgctga gaaaaccgtt gttaataatt cttcaataaa
    22621 acgataacga ttttccaagc taccgcccca ttgatcctct cgtctattcg aatgcggaga
    22681 gaaaaattgt tggatcaaat acgtatttgc tccatgcaac tcgattccat caaatcctga
    22741 ttcaatcgct ctacgtgtgg ctttgccaaa tgcttggatt gtttcaacga tttcttcttc
    22801 actcattgct cgtggtgttt ccgcatcagg acgttttgct gcaactgcac ttgcagatac
    22861 aggctgttct ccagataaag ttgctcgact tgtcattcgt ccaccgtgga aaatctgaat
    22921 aatcgctttt gcgccttctc cttggattgc tttcgctagc tcacttaatc gaggaatcat
    22981 ttcatcatga gcgatgctga gttcacctgg ccagcctttt ccgatatctt ggacatttgc
    23041 agcaccagta ataacagcac caagaccttt agaacgttgt ttataataag tgatctcatc
    23101 ttcggtgatc actccattga aaaagctgag ttgtgtcgtc ataggcgaca gcatcaatcg
    23161 gttcttcaat tgaactcctg aaggtaaagt aattgattga tcaaattgtg acattgtcat
    23221 tcctcccata attcgctctg attaacctat cgagaatcat ctatcattaa accactcgat
    23281 tagttgtaca tgcaactaat ttgcttaaaa aattttattt tttttgtaac ttatctgttg
    23341 catcattcat tgtctgggct aattctttac ttgcttcatt tcccaaaact gctttgtatg
    23401 actgatgaag ttcttcccag ctaccatcga cttccttagc aaattggcgt ccttgttctg
    23461 taagataaac taatgacaag cgaccttcgc taactgtttt gacgtatcct ttatattcta
    23521 atttttcaac aaaacgagca atcgttgatg gggcggaata gagcatttgt gcaatatctt
    23581 tttgtggtat tccatctttt tcttttaata tcaataaaat aaaaccataa gttggtgcca
    23641 gacctgtcct tctaaacgct tcatcagcta atttattcaa catgcgatct agctttttca
    23701 cgctaaagaa aagacattca tctaacgctt tttcaattgg cttttccatt tcatcccctc
    23761 cattggcgta catatcatag tatgattttt attttttgtc aaaaaacacg gaataatgtc
    23821 cgcctcctct gtctagattt gaaaaaaaga agtagccatc ttcaaagaat ttcggcttgt
    23881 tttcgacaaa actccttctg gaacaccgtg aatcagaggt tgtgaaaaag tcgtttagtt
    23941 ttgagtatga agaaaaattt tgtgaaaatg gctttccaca tttttgcata aatttggctt
    24001 aataccgaaa aactggcttt tgaacaccgt ttatatcaga ggttgtgaaa aagccgtcct
    24061 gcatcaagta ataagaacag ccaaacaaaa atagttcctc atattttgtc gttttttgag
    24121 ctaatgccga tggtcagctg attgtacacc gtttattgag aggtcgtgag cctgccgttg
    24181 aactccgagt cacaaggagc aatttcaaaa aacagcttct catgttttat gactatggtg
    24241 acttgtgatc gaggagttgg caggagaata ctgtttattc gtaaagaagg ggctgtgaca
    24301 agacttttgt cacagtccct tatttccttt tattatagga tttcgccatt tgattggatg
    24361 acttgtctgt accaatcaaa tgattttttc tttaaccgtt tctttgtgcc attcccttcg
    24421 tcatcttgat caacatagat atatccataa cgttttgaca tttgtgaagt agaagcgctg
    24481 acgatatcaa tgcagcccca cgacgtataa ccaaataaat ctactccatc tttgatagct
    24541 tccttcatct gctcaatatg ctgcttcaaa tagtcaatgc ggtaatcgtc ttgaacggtt
    24601 ccatcttctt ctactttgtc tatagctccg agtccgtttt cagctatgaa cagtggtttt
    24661 tgatacctat catataattg attcaaagca attctcaaac ccttaggatc aatctgccag
    24721 ccccattcac ttgatggaag atacggattt ttaattcctc cagttattaa gtttccttgg
    24781 accccttccc aattttctgg atgagcactg actgcactag acatgtagta gctaaaagaa
    24841 acaaaatcaa cagggtaggc tgctaagatt tcttcatcac ctacttctat ttttaagttg
    24901 acaccttttt cttcaaacat tcgttgcgta tacgctggat aatatcctct tgcttgaaca
    24961 tcactaaaga acaaagaagt tctttgctgc tcaattgttt tttgaatatc atccggatca
    25021 caagaatacg gaacaagaag ctgatagctg atcatacaac cgacttgtga acctttgata
    25081 atttcatgac aagcttttgt agctaaagca cttgctataa actgatggtg tgcaccttgg
    25141 tatttcgctt gttccaaatg gggatggttt tcttcaatta ctcctacgct aacaaaaggg
    25201 tgatgtttta cacagttgat ctcattgaaa gtcatccaat attttacttt atttttatag
    25261 cgtttgaata ctgtttcagc gaaacgaacg taaaactgaa taagctttcg attaggccat
    25321 ccaccatatt ctgtaaccaa atataacggc atttcataat gggaaagaga aacgactggc
    25381 tcaatcccat attttagaca ttcgtcaaac acttgatcat aaaaaaataa tcccgcttca
    25441 ttgggtgttt cttcatcacc atgaggaaaa attcttgtcc atgcgataga catccgatag
    25501 catttaaaac ccatttcagc aaataaacgg atgtcttctt taaagtgatg atagaaatca
    25561 tttccgtgtc ttttaggata ataatacttg ctgttgggat cctgcgccgc ttttacttgc
    25621 tgatgcgtca tcatatgcca ttgatctgtc catttttctt taggtaaatc gctgtcgaat
    25681 gtatacacat cagacacaga taatcctttt tcatctacat cccatgcacc ttcacattga
    25741 ttggccgctg tcgctccgcc ccataaaaat ccttctggaa attttttcat atcgatcccc
    25801 ctaaacttgc tagttttctt gtttgattat ttttcttatg actagtaatg atatttttga
    25861 atctactact atttgtaaca ataaccattg tggtcggatc atatccctct ttgattaatt
    25921 cttgcaaatc tacttccatc aataaatcgc ctttacgtac tttttttcca tcagatactg
    25981 cactttgaaa gtatttgcca tttaaattca ctgtatcaat accgatatgg atcagtatct
    26041 ctgctccttc ttcactgact accccaattg catgtttcgt tggaaaaact gttgaaactt
    26101 ccccgtttac agaactgtag atttttccgt ccgttggaat gatcgcaaac ccttctccta
    26161 aagatccact agcaaatgtt ggatcgttta cttcagataa atcaattatt tcacctgctg
    26221 caggagtact taacactata tcctttgagg attcttcttc gatttcatcc ttgtagccga
    26281 aaagaaaagt tagcatacag gaactgatca tcgctaagaa ataactagca actagccaaa
    26341 caaatccttc accaatataa atcgggaaag acaaaatact tcttgtagca actgccaaag
    26401 caccggctcc tgcggcacca gctactccac caaatatagc tccacatata caagcaatgt
    26461 aaaatggtcg tttatattta agagtcacac cataaacagc aggttcagtg attccaaata
    26521 atgctgtgat aaatgcagat aaagataaac tttttacttt cttatttttc gttttcaaaa
    26581 ataccccaag tactgctcct gcttgcgcag ctacggcagt catacatgct ggacctagcg
    26641 tatcacgacc gtagaaagaa agattgttgg tcattactgg tacaagtccc caatgtaacc
    26701 caaaaatcac taacacttgc caaaatccgc cgataacagc accagataaa acagggctaa
    26761 agttataaac agctaaatat ccagctgcaa tcagctgact tgcataatct gcaattggac
    26821 ctacaactaa taaaataatt gggaccatca cgatgataca aatgaatggt gtcaataaat
    26881 ttcgaatact ttcgtgcaag cttttgcgaa tcttaggctc aatataagaa agaacccata
    26941 cagctaaaat tgctggaata accgtacttg tataccttgc tagaatcacg ggcatcccta
    27001 gaaatcgcaa ggttatgcta tcactatatg cactgacaat cgtaggataa acgagagctg
    27061 ctgcaataac catagcgatg aaacgatctg tattgaactt ttttgcagca gtatatgcca
    27121 aaaatatagg caagaaatag aaaaatgcat cagctgctgc attcaaaatg atataagttc
    27181 ctgattcagt agttaataaa ttcaaactcg tacataaggc gagtactcct ttgatcaaac
    27241 cacttcctgc caagacgcct aaagtcgggg caaaaacagc ggcgatcatg tctataaaag
    27301 agtttaaaat gccttttttc tcagttcctc tctttccact agatgcatca tcttctagtt
    27361 tcatttgttt ggataatacg tcatacactt catttactgc atttcctact acgacttgaa
    27421 acattcctcc agcttcgact acagtgatca caccttctag ttgctctacc ttctccttat
    27481 ctgccttctt attatctttt agggtaaagc gtaaccttgt tgcacaatgg taaacattgc
    27541 tgacattttc ttcgcctccg acaaatcgga caatatcttt cgctaattgt gaatagtcca
    27601 tttttacttc ccccttgtta attaatgccg tagcaatcct tcttacaaga taagtatagt
    27661 tcttagaaaa cggtttctcc attcaattag taccccttct aatagcgaac gaaactgtac
    27721 aatagtgtgt atggtttact tagccgagca aatcactcac tgcttgaaag aaaacaagta
    27781 tatcgtttct cttatagtca aaggagacaa aaaaaacacg cttgatcttc ctttgctgat
    27841 gtataagtgg tatctcaagc tgatactctg tgatttttgt ttctacttgg cttttgataa
    27901 gacagcacaa caaagtctcg gcatttaacg gaagcataac taggtcagaa ctaggagaga
    27961 ttagttgctg aatggaatct atctttttat ggatttttat ttgattttct tctttgtagt
    28021 aatgatcaag cagttttgtt ttcttttttt cttcaactat ggtcagcttt tcacgcaaaa
    28081 tctgataaat ctcccaagat tcaaactcat ctagaacact aggtagataa atcaaatcat
    28141 cccactcaaa aataagctct tgctcagtag tcaaaattgc atcatattcc gttattttgt
    28201 ttgattgatt tttgaaaaga tacactggtt ctatccggca ttctttgatc cacatgttca
    28261 tactttgttg aatctgtttt tttagtgtat tcaaaatact aattggctgg ttactaacaa
    28321 tcagtatctc cccttgtttt cgaaaaggat tcaacgcttc ggcaagatac aaaacaaaat
    28381 aagcgctttc agctaaattg ggacgtaatt gaaaatgtat tgggaagaaa cgccgaatta
    28441 aataaatttc taaaggataa cgggagatga tcttttgtac aaaatgattc atggtattat
    28501 gtccggcatc catcctcgtt ttcatttgtt tgatatgggt tagcaaaaat tctgtttcat
    28561 ggaacaataa cgaaacagga agatctaaaa aacgaataat cgtttgctca aatgctctta
    28621 gattcttctc tatttctttg tttttttcat cattaatcat caaataattt aactctaata
    28681 gtcgattttc aattgcttgt ttttcttgat tggaaaaaga atagggtagt tctgatgata
    28741 acttttttgt gagggtctct accatcggaa taaataatgt atacctgttt gttttttcta
    28801 aaagttcttc tcttcgaaga atggtcaaaa cgagataccg agaaaatcgt atgaagtcct
    28861 ctcctgaaac aatataatta tatgatataa gaacctcttt taatgtcttt tggacagtcg
    28921 gcatgatatt ggaaaaaatt gtactgattt gttgaggaaa ccttgcgtgt tggatcaccg
    28981 tttctgttcc aacaagagat aagaaaattc tttttgaatt ttcttcagca atgattttta
    29041 gtccttttgt cgacgaaact tccagttcta atttaggatt gcgctcgatc caacgcaaaa
    29101 tcgtttttat ttgtaatgat acggctgttt ttgaaagaaa gaaaatgtct gctatctttt
    29161 gcatactgac ataccctttt tgaaacaata agtacgtcac aatagttgaa gctcggaaat
    29221 ccatgctgga atagatttta tgtttcgtca cctcttgtat gatttgctgt ttagtttcag
    29281 attcaattga ttctaaaaaa taacctgaac gtttatttga ctggataatg attttcttct
    29341 cgtagatagc aagcatattt ttgatttcag tttggatcgt tttcttgttg gtacctaaag
    29401 cttcagctaa ttcttcgctt gttatccaat gattcttatt tcttaatact acaagaattt
    29461 ggagttgtcg tttatttaag tccatttgct tacacctcct ctttgttata aacatacagg
    29521 aaaaacctct caaatgacaa tagtcacttg cagaggttta aaaatcattt gaagaaagat
    29581 ctatattcaa ggtttttacc attagactgg ataatttgtt tataatattc aaaactatct
    29641 tttttcaatc tcctaaagct tccgctccca tcattttgtc tatccacata aataaagcca
    29701 tatcgcttcc tcatttcccc tgttcctgcc gatacgatat cgattggtcc ccaccataag
    29761 taaccaatga tttggcaacc atcttttatt gcctcagcaa tttcctgaag atgtattttt
    29821 agatactcac aacgttttgg atcgtggatt cgattcaatt catctggtgc ttcatctagt
    29881 ccaatgccat tttcaactat gaataatggt aattcataac ggtctgtcaa ttcattcatg
    29941 acataacgaa accctagagg atcaattggc caactccaag gttcgggaga tttttccttc
    30001 aaaaagggat tatcgaaacc atttgctccg ccagtatcta cgcgcatttc tgcttctttt
    30061 ttatagacag cgctacgata ataactaaat gcaatataat ctgccgtgtt agcttgcaag
    30121 cactgttcgt ctccatctaa cattactggt gcacagtctt cttccttcca tgtcctgtaa
    30181 atataagctg gataatgccc tcggcacatg acatcggaga aaaaccatga attgcgttta
    30241 aattgcagtg aaccaaatac attctctgga tcacagtcat atgcataagt cgctaatgca
    30301 gaaaagctac acataatacc catcttagca tttggatcga ttttctttaa taattgagta
    30361 gcacgggcgt tcgctagaaa catatgatga caggcttgat aaatcacttt ttttgttagt
    30421 gtagcaatgg gttcaagttt atctctagca gggaatgtaa ccaatccagc agctgcataa
    30481 ggcatacgaa aagcattatt gatctcatta aaggtcatcc aatatttcac tttcccttgg
    30541 tagcgttcaa agacggtctg cgcataaaat tcaaaaaagt cgattaattt tcgattttgc
    30601 caccccccat actctcctac caatgctaag ggggtttcat aatgactaag agtaattact
    30661 ggctcgatat catatcgatt taatgtattg attagtctgt cataaaatac gagaccagcc
    30721 tcatttggct tctgttcgtc tcctcttgga aatattcgtg cccatgagat gcttgttcga
    30781 aaagctttta aacctaactc cttcagtaaa cgaatatcct cttcaaatcg atgataaaaa
    30841 tcaatcgctt catgagaaag ataatggagt gattcgtcta aagcaatctc ccaaatcttt
    30901 ttttcttcat tccactgaat actaggatgc tggttcatga tcccgataag cgtatctgtc
    30961 acattaggta atttgccgtc ttccaaccat gcaccttcac attgattagc agctactgct
    31021 ccgccccata aaaaattttc tggaaatatt tcctgatcca taactatctc tcctcgctat
    31081 caacaatttc ttacttgaag tatagctcga ttcatcaaaa caagatattc taactatttc
    31141 ggcagaagag aagaaaaaaa ttgaatgcta ctcttcattt ttaatattta tatataacaa
    31201 atacataaga cgatccccga aagaaaacac tcgttaacca tctaagcaaa cgatttattt
    31261 tacttccaac acaaatgctt cataaggtcg taaatcaact ttgccgtctg gtaactgttt
    31321 cagaggataa tttgacacaa taacccttaa agcttcacgt ggaatagtga acgtctgctt
    31381 ttcactggac atattacaaa taacgagcca ttgctggtct tcatatatgc gagaataggc
    31441 aaagacatgc tcaggcgtct tttccactaa ttcatagtct ccccagacga tgatgtcatg
    31501 ttttctacgc aaatcgatca atttcttgta catataaaaa acagaatctt gatctgctaa
    31561 cgcttcttct gcattgattt ctttataatt tggattgact cgaagccatg gaattccagt
    31621 agtaaaccca gcatgttcac gggcgttcca ttgcataggc gtcctagcat tgtctcttcc
    31681 tttcgccttg attgattcca agatttcctc ttttgtaaat ccagaagaaa tcctttcgtt
    31741 atacatattg attgtctcaa tatcttttgc ttccgcgata tcatcgattg ggcaattcgt
    31801 catgcctaac tcttctcctt gatagatata aggggtgcct ttcatcatat ggagcaagat
    31861 agcaaacatc ttggcacttt cttttcgata cacatcgtca tttccccaac gagaaacaat
    31921 tcgcggaaga tcgtgattat tccaaaataa actattccat ccttcatcac ctaaagatgt
    31981 ttgccatttg gataatactt ttttcagttc agagatactc aagggtttca aatcccattt
    32041 gtctttgcct tcttgctgat ctaatcctat gtgttcgaat tggaagacca tggataattc
    32101 atttctttta ggattggaat aaagtttagc gatttcggta gttgctcccc atgtttcgcc
    32161 aacagtcatc agatttttgt caccaaaagt tgcttgattc atttcctgaa gatactcatg
    32221 cagtttaggt ccattacttg tgatttcttt atccggttct ttgccaatca tatcgatcac
    32281 gtccatacga aagcccccaa tacctttatc gatccagaaa ttcatcattt gataaatctc
    32341 ttggcgaacc tgtttatttt tccaattcaa atcaggttga cgcttgctat acaagtgcag
    32401 aaaatattgc cccgtttttt catccaactg ccaagcagaa ccactgaata ttgagcgcaa
    32461 tctattaggc tcttttccat tgaccggatc acgccaaata taatagtccc gataaggatt
    32521 ttctttattt ttacgggcct caatgaacca cggatgttca tcagaagtat gattcaccac
    32581 cagatccatg atgatacgga tatttctttt ctttgcttct tcaatcagtt cttccatttc
    32641 ttccatcgta ccgaattctg ccatgattgc ttgataatta ctgatatcat acccattgtc
    32701 atcattcgga gactcgtata ctggagaaag ccaaattgca tcaataccta aatacgccag
    32761 ataatccaac ctggaaataa tcccccgcaa atctccaata ccatcttgat ttgaatcttg
    32821 aaaacttctt ggatagattt gataaacaac tgaatggtgc caccattttt gttccatgat
    32881 tttcctccta taaatggatg tttctatttt ttgcttaatc atttatttta gtctctctca
    32941 ctaaaaattg taacgctttc aaatagctat tgcaccttgt tatcggtaac agaataaaac
    33001 aaaaaaacag ctgaggaaat tccctcaact gttttttgaa aggattgatt tatgcaggga
    33061 agaatgctga tttcacaact tctttataaa cggtgttcac tcagctgtcc cttggtatta
    33121 gctcaaaaaa cggcaaaata taagaaacta ttttttcgtt tttttctcta atactcaggc
    33181 cagaacgctg atttcacaac ctctttattt cacattcaat atccacatga atcccacaaa
    33241 tacaaagaac agaccataca tgattggaga tacttctttt cctcttttag ctgctaacat
    33301 ggtaattgga tagaagataa aacctaatgc aatcccatct gaaatactat aagtcaaagg
    33361 catgccgatc aagatcaaga atgaagggat cgcaatctct aagtttcccc attcgatttg
    33421 tcgcaaagat tgtgccatca atactcctac cataatcaat gctggtgcag ttacttgtga
    33481 agtaacaact gatagtaatg gagaaaagaa caagcctaac aagaagaaaa tccctgtagt
    33541 aatagctgtg atacctgaac gtcctccaac ggcgatacca gctgatgatt ctacaaatgc
    33601 accaacagga gaagtaccaa ataaggaacc cgcaagcata gctgttgaat cagaagctaa
    33661 agctttccct acacgaggca tcttgttgtc tttcatgaaa cctgcttggt ttgctaatcc
    33721 aactaaagtt cctgctgtat caaagaaagt cacaagcaag aatgtcagca ctacgaccca
    33781 catttgaaga gaattgatgt cccctacatg ttttaatgca actaaaaatg ttggtttcaa
    33841 acttggtgct gcggcaacaa tttcagaagg cattggaatc aaacctgtga ccagacctaa
    33901 aatcgttgta gctgccatcc cgatgaagat gccacctggt acacgccgaa caagcaagat
    33961 agcagttacg actaatccaa aaatcgtcaa ccaagtagaa ccaacgttga gtgatcctaa
    34021 tcctactaat gtagattcat tcgaaacgat gatcccacct tcgctcagac caaggaaagc
    34081 gataaataga ccgataccgc cagaaatcgc atatttcaaa tctgctggga tcgcatcaat
    34141 gatcatttcc cgcagcttga agattgtgat caaaataaaa atcaaagaag caacgaatac
    34201 gccggctaat gctgtttccc atggaatccc cattcctaaa caaacagaat aagcaaaaaa
    34261 agcattgatc ccaagagcag gtgccgttcc aattggataa cgggctaata cccccattaa
    34321 aacacagccg atcgcactgg ctaatgcagt cgcagtaaac actgctcctt catccattcc
    34381 agaagcacct aatacagttg gatttacaaa taagatataa gccattgaga taaatgttgt
    34441 aaatcctgcc agtacctcac gttttaccgt ggtgttcaat ttttctattt cgaagtagga
    34501 aagtattttt tccttcatct tccatccccc taaatccgaa cattataaat aaagtccgtt
    34561 aaaatttcga tttattaata aaacaacgtg ttttatcata tactaaaaca aaacataagt
    34621 aaacaaaaaa ttcggatttt aagcaaaatc cgaacgttcc atctgattaa atccctttat
    34681 ctcaaagttc atgaacattc actttggatt acaagaattt ctttattgtg ttaataaagt
    34741 ttcttcattc tagtctcaaa aatcaatagt gtgatttgat tttaccattt cgtttctgtt
    34801 cttctttgat gaattctttt gtatcgacag gattcagatt atttagtccg ccaaaacctg
    34861 tatcgaccat ttttccccag ccttttgtct tcttttttcc tgatttgaga ttctttgctc
    34921 gaagcttctc actatgtttt gctcgccgat acttcaaata accttctaat cttcccatta
    34981 gatccactgt ttttcctcct ttttattcaa tcagacgact cagcttttcc tataacaaaa
    35041 taattgcctt cagggtcaga aaaattgaaa gtcaagatgc cattgttatc tgtgatttct
    35101 cctggttctt ccatgttctc gtagatagtt tcaaattcat ccgtaaaaaa tactaaagaa
    35161 ggcggtggtc ctaatacctc tggtgaaaac ttctgcacaa atacttttgg gaatacgcct
    35221 aattctaatt cttttgagat gctgagtaca atactcttaa attcttctgg caattcgatt
    35281 gtttcagaaa tttctgcacc aattttttct acaaagaatc ggcaaatcat ctctacgtca
    35341 ttgacataaa gcatcacacg tgttttttgt atcatcattt gacccttctt tctatttgat
    35401 tttctttcct gtctataaac tgattccatt atatcacgaa agcgcaaaca ccttgtgttt
    35461 gatactattc aagaaaaata aagcaatagt ttctaggatt gctttcctcc acaaactttt
    35521 tttacaaaaa aaaaacggca atgaatgccg ctcttcttat ttcgattctt ttataattct
    35581 gcttctgatt catctttggt tagtgtgtgt ctaacactag ttacgccaaa tgtttgatcg
    35641 attttttcct tcaacgcttc gattgcctgt gcgccacgtg ccgcttgttc agcattgatg
    35701 gattcaatga ccagcaccgc tgcttgtgtt tcttccgtca gcatcgttct ttgggcttca
    35761 cgccagttga gacagcgaca gatcgctcct tgttcatcat agtagatgat ttcttccgga
    35821 agtgccggag catcggaatc tgcacctaaa ggcaggaatg attcgccacc ttgggctttt
    35881 cctaaatgca gatttcctgc gatcttttcg atatcttctc cgccgcaagg tacgccaaaa
    35941 gacaaggaaa tactattata gatatctaca agcggattga ttgggaaaaa ttcccgttct
    36001 tgatttgcac gtttcagtaa agcttcaata gaagaacgtg cacctttttt agtcttgaat
    36061 gtggtaaatg cctgacgcca ctgagcaatg acttcgtttt ggctgaatgt ttcttctgta
    36121 aggtaatttc ttgcttcttt tgctgcctca ttcaataatt gatgatacgt ttcttcactt
    36181 tcttttgtta cgtggttatc gataccattt acggataaga tttcgatggt tgcttcagga
    36241 aatacttccc agaaattggt atccactatt acttttttca tctgttcaca cctcttgatt
    36301 agaataattg cttgatgaat agatagtctg tttggggatc atctcccata taaaaaacat
    36361 gttcgctgta tttgataaat ccttttttac gataaaaagc gagcgcaaca tcgttgtgtt
    36421 cccaaacacc gagccaaatg gctgtttttt gcttttcttt tgctttttca atagctgtat
    36481 ctagtaaaaa tgtaccgata ccttgatgtt taaatttttt tcgtacatag attcgctcga
    36541 tttctaaaaa atgatcagcc atttcctcag attgactcgt tcctgtattt agtttcaggt
    36601 aacccgcagg ctcttgatcg atactcacta ggaaaaaatc ggtttcttta tcttttagct
    36661 cttctgtaag tttttctagt gcataagctt gtgtcaagta atcagctaaa tgctccggtg
    36721 aattatcttt tccaaatgta tctgtaaatg tttcgatact aatttgttgt agtaagtcta
    36781 catctgaagg cgtcattttt cgaatttcgt atttattcac ggcttcttcc tttcaataga
    36841 tccttttatt gccttttttg acaaaattcc aatcttcgct aacattcttg catactttat
    36901 ttaaaagata ttccaattgt tttgcttctt cgtcagaaag accatttaat gcgactgcat
    36961 tggagtagtt gttttctcga atgatgaaag gatagatttc tgctcctttt ttagtcggat
    37021 agagtttctt gattttttta ttttctttat cttccaaacg ttcgatcatc ccattggatt
    37081 ctaatttctt gattgctcgg gcagtcgttg tacgatctac tttgatcatt tctgctagtt
    37141 tttcttggat gattccagga ttttcgcaaa tacgtactaa ataaagatac tgtcctcttg
    37201 ttaaatcgac ttctttgaat tcgatattgc taatagaatc tagcgctcgg gcaatcattc
    37261 cgatgtctcg aaggatttca gccatttcat cccaccttta ttttgtttgt aagactagtt
    37321 cacctctttt tgttgcaaat gcaactattt tcaataaaaa aatcaagttc ttttttaata
    37381 actagtaaag tatattcttt tcttctttta cgaaaaagct caatcacaat agatctggtt
    37441 tttgttttct aaaataagta actgtttctt cgttgttcgc ttttctgata gctttggcaa
    37501 ctcttctata ctaaaaaatc ccatgttgga agtttcatta ttctcaataa acttggcctc
    37561 ttcaccgtgg atcgcacaag caaaaacaag tttataatac tgaaaaagct gtggaatatc
    37621 tttttgttta ttcgtatcaa aaactgctcg caactgagta gtttctactg ttaatcctgt
    37681 ttcttcatag acttctttac gcacattttc ttcaggagac aatcctattt cagcaaatcc
    37741 accaggaagt gaccattctt tcgtccgttt atcttcgatt agtaggacct tgccatcttt
    37801 tttgatatag gcacgaacat ctacttttgg tgttggatgt ccttctccaa tcgttgtgag
    37861 cgcttttatt tcttcgaaag tttcttttcc tatttcactg atcagttcca atgaaatcgt
    37921 ccggatctcc tcaaaacgct ctttatcaaa tacatctttt ccataatata gaccagcttg
    37981 agaaagtgcg agtagtcttt tgtattgagc caacttcttg ttcattctct tcccttcttt
    38041 tttagcaaat gtatgactat gtaatcaagt atagaagact ggtttaatta cggcaaataa
    38101 atataatgaa atctgcaata aataaaagaa tgttaaaaaa ctcgagaaca aaagacattc
    38161 gacttctctg gggctgtgaa taaatggcgt tcaaaagctt gcatctgcgg taataagccc
    38221 caccaaacga aaaatatgac gagctattct tgtctggctg ttcttattaa ttgaggcagg
    38281 acagcttttt cacaacctcc ccactaaacg gtgttctcct accaactcct cggtcacaag
    38341 tcacaatatt catcaaacat gagaagctgt ttttttgaaa ttgctccttg tgactcggag
    38401 ctgaacgaca ggctcacgac ctctcggtaa acagcgttca aaagccaatt tttcgatatt
    38461 aagccatttc tttgttactt attgtcattg atttcttttt ctaatttatc caatagtgga
    38521 gtgtaatcca cttgagtata gtctgttaca tcaaattcat atagtgttcc taactggaat
    38581 ttgtcatatt ttcggtcggc gatgatcttt ttgaatgctt ctttcgtaat atgattggtc
    38641 cctttcgttc gatcttccaa tacgtcacca tagtgataat gatccatgat atagctcaaa
    38701 tgacgatctg attcacgatc acgtttgtac cgacgttgcc ataacacttc aaagtctgct
    38761 gttaggcgaa tcgtgatcac ctgatatccg tatctatctg ctaattcttg gagtcgtgct
    38821 ttttgcttat cactaaaagg atactcggac acaatcactt tctttcctgc ttccatatac
    38881 aaatcaagtg ctttgtaata gaaaggataa actttcgtcc tttcttgttc cgcccgttct
    38941 tccaaattgt taaacccgat ggattctgca tagtattcct tgatttcatc gagcgtgatg
    39001 gcatacatat ctggaaatcg ttcccgtagt ttattcataa gatatgtttt tcctgtacca
    39061 ggactaccag ccagaagaat gaaatacttt ttcatctcat ttatttccta agttaattgg
    39121 actttccaag ttcataaaca gtccaatctt atgcttgaca atttctttta ctgcttcatt
    39181 tgctggaatc acattatggc gaagagactt gttgatttct gtttcactaa aggtttcttt
    39241 agctactttc gtccagttga ctaaaagctc cgttccaaca ttgaatttgc ggatcccgtt
    39301 attgatcaat tcaggataat cttcttcttt cacacctgtt cctccatgga taactagggg
    39361 gacatcaact actgcatcga tttctttcag caagggaatg tttacttctg tttttgattt
    39421 gaattgtcca tgattcgtac cgatagcaat agctaatgca tccacgcctg tagcttctac
    39481 aaatttcact gcgtcttccg gtcttgtata taccttgtca ttttcagcga catggattcc
    39541 ttcttctgta ccgccgattg ttccaagttc tccttctacg gaaacaccgt gcgcatgggc
    39601 atattctact actgcttttg tcttcaggat attttctttg aaaggcagat gggacccatc
    39661 atacatgaca gaagtatatc cgctgtcgat tgcttctttg atatcatcaa agtctcgggc
    39721 gtggtctaag tgcaagactg catcaaccat ttcttcttct gcaatagctg tcattacatt
    39781 gactaacaca tgcatgccga tatatttagc ggtatcgaca cttgtttgaa taatgatcgg
    39841 cgaacccatt tcttttgctg cgcctagcat agcaggaagc atttctaggt tatgcgtatt
    39901 aaaagctcca actgtaaaat tcaactcttc tgctgttttt gttacttctt tcaatgttgt
    39961 atacatcttt tattgccacc ttatcttttt atttgtttcc tttgatcgtt acttgttttg
    40021 caatttgtcg catctcatcc attttgccca tgtagtaatc gatcccttga tcatcaccag
    40081 cacgagcagc atctgcacgc ttctggttat agaggctcat cagtttttta tacccatctc
    40141 cgatcgtctg tttattttcc aaactctttt ctaaatattg gatggcttga tcgttcacat
    40201 acaattcaga aaatttaagt ccaacttgtt cataaagttt cgctaattta tgcttatctt
    40261 gggtagtgga aatctcttgt ttcaattgtg taatctctga ttgaatcttt tcggtcgctt
    40321 ctggcgatag ggctttttct tcttttgcca gaactttttt cggttctttt tcttttttct
    40381 taaaaaatcc aaacatcatt ttccctactt tcaattaaaa tgctttcaaa actgggctta
    40441 agatccaggc atataagagt gctgcggcta ctgtatctac atcggttgct gtagcattgt
    40501 tgaatcccat cgaattaaag atcgttacta gcaaagcggg caataatgta ataaagaaac
    40561 catgagcaat accgccaatg atcgctcctc ggcgtccgcc aactgcgttg ccaaatatcc
    40621 ctgctgtccc tccggcaaag aaattggtta gcataccagg taaaatcatc gctaagccga
    40681 acatgggcaa agtaaacatg gcaataatcg taccaatcgt tgtcgtaatg aaacctaaaa
    40741 tgacagcatt tgggctataa ggaaagaaaa ctggacaatc taaggcaggg atcgcatcag
    40801 gaactaattt catagctatc cctcgaaatg ccggcacgat ttcacctaac agtagacgta
    40861 caccagctag caatacatac acacccacta cgaattggat ggcttgtaaa aaggcaaaca
    40921 tgatatagtt ttgatctcct gataattcag aagcgaatgg ttcgcctgca aataaaacag
    40981 tgatgatata tagcgggacc atcacgacca tgacagacaa ataagtatct tgcagaaatt
    41041 cgaaatgtgt cggcaacttg atatcttcga tcgacttttt attttcgcct ttctcaccaa
    41101 aaagcttagc aacaccggct tcaaataagt acccgatcgt acaaaaatga cctaaagcaa
    41161 tatcatttga tccagtgatt ttacgaatga tcggttgcgc tactgccggc atagcaatgg
    41221 cgaaacaagc tccgataaaa ccaccgacca agatcaatac gatccctcgc agaccggcaa
    41281 aatagccaaa gactgtcgtc atcgttgcca tccataaaat tgcttgacca gttaaaaaaa
    41341 tgtacttcca ttttgtgaaa cgagccaaaa taatattgaa tacaaaaatg gctaagaacg
    41401 tcagtgcaat atctctacct aaacccaaat cattcattgc ttgaccattg atagattcaa
    41461 tagaagggat gatcccttgc atatgaaatc cttcagtaaa gattttcccg aaataagtta
    41521 aacttccaac gatgatgctc gaaccggcac tcaacacttg aaatcctagg agtgtcttta
    41581 acgtgccaga gatgacttgt cctgctgatt tcttctgcag tagtagaccc aacatagcaa
    41641 tcaatgcaat ggtgatcgat gcctgagtca aaatattttc gatgataaaa ttgataacgc
    41701 cca
//
LOCUS       DAZBTF010000018        39518 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000018 DAZBTF010000000
VERSION     DAZBTF010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.018"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            63..920
                     /locus_tag="ACHGNS_001027"
     CDS             63..920
                     /locus_tag="ACHGNS_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFN6936989.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            1035..1955
                     /gene="pstC"
                     /locus_tag="ACHGNS_001028"
     CDS             1035..1955
                     /gene="pstC"
                     /locus_tag="ACHGNS_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFN6936990.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPIVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTITTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            1955..2839
                     /gene="pstA"
                     /locus_tag="ACHGNS_001029"
     CDS             1955..2839
                     /gene="pstA"
                     /locus_tag="ACHGNS_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFN6936991.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2854..3660
                     /gene="pstB"
                     /locus_tag="ACHGNS_001030"
     CDS             2854..3660
                     /gene="pstB"
                     /locus_tag="ACHGNS_001030"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFN6936992.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3676..4434
                     /gene="pstB"
                     /locus_tag="ACHGNS_001031"
     CDS             3676..4434
                     /gene="pstB"
                     /locus_tag="ACHGNS_001031"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFN6936993.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4447..5124
                     /gene="phoU"
                     /locus_tag="ACHGNS_001032"
     CDS             4447..5124
                     /gene="phoU"
                     /locus_tag="ACHGNS_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFN6936994.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5701..6393
                     /locus_tag="ACHGNS_001033"
     CDS             5701..6393
                     /locus_tag="ACHGNS_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFN6936995.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            6390..7157
                     /locus_tag="ACHGNS_001034"
     CDS             6390..7157
                     /locus_tag="ACHGNS_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFN6936996.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7257..8825
                     /gene="liaX"
                     /locus_tag="ACHGNS_001035"
     CDS             7257..8825
                     /gene="liaX"
                     /locus_tag="ACHGNS_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFN6936997.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            9122..9442
                     /locus_tag="ACHGNS_001036"
     CDS             9122..9442
                     /locus_tag="ACHGNS_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFN6936998.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPFARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            9442..9792
                     /locus_tag="ACHGNS_001037"
     CDS             9442..9792
                     /locus_tag="ACHGNS_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFN6936999.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            9959..10897
                     /gene="hprK"
                     /locus_tag="ACHGNS_001038"
     CDS             9959..10897
                     /gene="hprK"
                     /locus_tag="ACHGNS_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFN6937000.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANKNGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10912..11742
                     /gene="lgt"
                     /locus_tag="ACHGNS_001039"
     CDS             10912..11742
                     /gene="lgt"
                     /locus_tag="ACHGNS_001039"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFN6937001.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11786..12811
                     /locus_tag="ACHGNS_001040"
     CDS             11786..12811
                     /locus_tag="ACHGNS_001040"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFN6937002.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12826..13764
                     /gene="galU"
                     /locus_tag="ACHGNS_001041"
     CDS             12826..13764
                     /gene="galU"
                     /locus_tag="ACHGNS_001041"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFN6937003.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13889..14329
                     /locus_tag="ACHGNS_001042"
     CDS             13889..14329
                     /locus_tag="ACHGNS_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFN6937004.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14332..14934
                     /locus_tag="ACHGNS_001043"
     CDS             14332..14934
                     /locus_tag="ACHGNS_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFN6937005.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(14993..16099)
                     /locus_tag="ACHGNS_001044"
     CDS             complement(14993..16099)
                     /locus_tag="ACHGNS_001044"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFN6937006.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16309..17310
                     /gene="ccpA"
                     /locus_tag="ACHGNS_001045"
     CDS             16309..17310
                     /gene="ccpA"
                     /locus_tag="ACHGNS_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFN6937007.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17647..19992)
                     /locus_tag="ACHGNS_001046"
     CDS             complement(17647..19992)
                     /locus_tag="ACHGNS_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFN6937008.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            20271..21173
                     /locus_tag="ACHGNS_001047"
     CDS             20271..21173
                     /locus_tag="ACHGNS_001047"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFN6937009.1"
                     /translation="MLLGSHVSMSGKKMLLGAAAEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            21386..23161
                     /locus_tag="ACHGNS_001048"
     CDS             21386..23161
                     /locus_tag="ACHGNS_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFN6937010.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(23227..24813)
                     /locus_tag="ACHGNS_001049"
     CDS             complement(23227..24813)
                     /locus_tag="ACHGNS_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFN6937011.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETVEEIE"
     gene            25064..25288
                     /locus_tag="ACHGNS_001050"
     CDS             25064..25288
                     /locus_tag="ACHGNS_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFN6937012.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            25429..27180
                     /locus_tag="ACHGNS_001051"
     CDS             25429..27180
                     /locus_tag="ACHGNS_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFN6937013.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSVKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTK
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIVMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPVKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            27180..28964
                     /locus_tag="ACHGNS_001052"
     CDS             27180..28964
                     /locus_tag="ACHGNS_001052"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6937014.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRTTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVLVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            29290..30141
                     /gene="licT"
                     /locus_tag="ACHGNS_001053"
     CDS             29290..30141
                     /gene="licT"
                     /locus_tag="ACHGNS_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFN6937015.1"
                     /translation="MLIEKVLNNNVVITRDERDREMIVMGKGLAFKKKVGDPILDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVHLNDSVYISLTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GAKSEDGEVSEELFQVIQKKYKDTFGCVEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            30237..32117
                     /locus_tag="ACHGNS_001054"
     CDS             30237..32117
                     /locus_tag="ACHGNS_001054"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFN6937016.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEAEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVSFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTEPAIYGITLPKK
                     KPFIYSMIGGAVSGIYLMASGTISYIMGGLGIFGVVNYITETDASGMFQAFIGIAIAS
                     VIGFSLTFFFWKDEAGETDAIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGI
                     LIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQ
                     QGERVRQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALITA
                     LT"
     gene            complement(32157..33059)
                     /locus_tag="ACHGNS_001055"
     CDS             complement(32157..33059)
                     /locus_tag="ACHGNS_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFN6937017.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKYLLLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNDVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            33290..34693
                     /locus_tag="ACHGNS_001056"
     CDS             33290..34693
                     /locus_tag="ACHGNS_001056"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFN6937018.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLSADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            34693..36126
                     /locus_tag="ACHGNS_001057"
     CDS             34693..36126
                     /locus_tag="ACHGNS_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFN6937019.1"
                     /translation="MGFRKDFLWGGATAANQCEGAYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSNN"
     gene            36271..37197
                     /gene="trxB"
                     /locus_tag="ACHGNS_001058"
     CDS             36271..37197
                     /gene="trxB"
                     /locus_tag="ACHGNS_001058"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFN6937020.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            37411..39138
                     /locus_tag="ACHGNS_001059"
     CDS             37411..39138
                     /locus_tag="ACHGNS_001059"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFN6937021.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            39153..39503
                     /locus_tag="ACHGNS_001060"
     CDS             39153..39503
                     /locus_tag="ACHGNS_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFN6937022.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
BASE COUNT        12645 a         6973 c         8508 g        11392 t
ORIGIN      
        1 cgttttcatc aagtaatgaa aatgataaaa taaacatact ttaggattaa aggagatttc
       61 taatgaaaaa acttaaatta ttcttaccaa taataggatt agctattctt ttatcaggct
      121 gttcaaagtg gattgatcga ggagaatcca ttacggctgt tggctcttct gctttacagc
      181 ctttagtaga aacagttgct gaaacttacc agacagaaaa tcctggaaaa ttcatcaatg
      241 tacaaggggg cggaagcgga actggtttgt ctcaagtaca gtcaggagca gtcgacatcg
      301 gaaactctga tttgtttgcg gaagaaaaat cggggatcga tgcctcagca ttagtcgatc
      361 atcgagtagc agttgttggg atcactccta ttgtgaacaa ggatattggc gtaaaagaca
      421 tcagtatgga aaacctgaaa aaactattta caggcgagat caaaaactgg agtgaagtcg
      481 gtggaaaaga tcagccagta gttatattga accgcgcttc aggaagcgga acccgtagca
      541 cgtttgaaaa atgggtattg gacggagaaa cagctgttca ggcacaagaa caagattcaa
      601 gcggaatggt gcgccagatc gttgccgaca cacccggtgc aatcagctat gttgcttttt
      661 cttatgtttc cgatgatgta gatacgttga atgttaacgg tgtagaaccg acagatgaaa
      721 atgtgacaac gaacaaatgg acgatttggg catatgagca tatgtacaca agagatcatc
      781 caaaaaaact gacaaaagaa tttttagact atatcttatc tgatgaagta caagataaca
      841 tcgttggaga actaggttat atccctgttt ctcatatgaa agtcgaacgt gattggcaag
      901 gaaatatcgt taaagagtaa tctttacaca aaatttacaa acttagctaa acttttacat
      961 gttattaaaa ccaaattgat gttcaatttg tacactgagt gagtatgtta aacctgatta
     1021 agggggagtc atctgtggaa gatgtgaaaa aagtattatt aacaaaatca aaacgttcaa
     1081 aaatggaaca gcgtggaaaa ttcatcagtt ttttatgtat cgcgctgatc gtgttggttg
     1141 tattatctat tttttatttt gtagctagta aaggattggc cacctttttt actgataaga
     1201 tcaatgtatt tgattttctg tttggtacag attggaatcc aagccaaatc ggagcaaatg
     1261 ggaaaccaat agtaggggca ttgcctatga tcacaggttc gtttattgtg acctttttat
     1321 ctgctatcgt tgccacgcca tttgccattg gtgcagcagt attcatgacg gagatctctc
     1381 ctaaaaacgg aacgaagatc ttacagccgg tcatcgagct gcttgtaggt attccttctg
     1441 tcgtttatgg ttttatcggt ttatcagtta tcgtgccttt tgtcagaagt atttttggcg
     1501 ggactgggtt tggtatctta gctggtacat ttgtgttgtt cgttatgatc ttgccgacta
     1561 tcacaacgat gactgttgat gcattaaagg cagtgccacg gcactatcgg gaagcttcat
     1621 tagcgcttgg tgccacaaga tggcaaacaa tctacaaagt cgtgctacga gcagccgtgc
     1681 ccgggatttt aacagcagtc gtttttggta tggctcgtgc gtttggtgaa gccttagcta
     1741 tccaaatggt catcggtaat gcagcattga tgccaacagg tcttactact cctgcttcta
     1801 cattgacgtc catcttaaca atgggaatcg gaaatacaat catgggaaca gttgaaaata
     1861 atgtgttatg gtcattagcg ctgatcttgc tattgatgtc attattcttt aatatcgtga
     1921 tccgtatgat tgggaagaaa ggagagctga aataatgaac gcaaaacgct cagataaaat
     1981 tgctacaggt gttttatata tagtttcggg gatcatcgta ttgattttag cagctttact
     2041 actttatatc ttagttcggg gaataccgca catttcatgg tccttcttga ccgaaccttc
     2101 caaagcctac caagaaggcg gtggaatcgg gatccagcta tttaactcga tttatttgct
     2161 tttgatcacg atgatcatca gttttcctat ttctttaggt gcgggtatct atctttcaga
     2221 atatgctgga aaaaactggc taacagatgt gattcgcacg tcgattgaaa tcttgagctc
     2281 gttgccttct gtcgttgtcg gtttattcgg tttcttggtt ttcgttattc aattccagta
     2341 tggtttctcg attatttctg gtgcattggc tttgactgta ttcaacttgc ctcttttgac
     2401 tagaacagtt gaggagtctt tacaagcggt ccattacaca caacgagaag caggattggc
     2461 cttaggtttg tcacgctggg aaacagtgat aaaagtggtg gttccagaag cgacaccagg
     2521 gattttgact ggtgtgatcc taagttctgg aagaattttt ggtgaagcag ctgcattgat
     2581 ctatacggct ggacaaagtg cacctgcgtt agattttacg aactggaatc cattaagtgt
     2641 ttcaagcccg atcagcattt tccgtcaagc tgaaacatta gctgtccata tttggaagat
     2701 caatacagaa ggcacgatgc ctgatggtgt ttctgtttcg gctggcgctt ctgccgtgtt
     2761 gattattgct gttttactgt tcaacttcgg cgctcgtgcg attggcaaac gattataccg
     2821 aaaaatgact tcagcctaag gagaagaagc aaaatgaaaa aatataactg gaatgaaaca
     2881 aatgtcatca ccatgccaaa agagaaagaa gtagcactat atacggatga tctacatgtc
     2941 tggtatggcg acaatgaagc aatcaaagga gtggatctgg aatttgaaaa aaataagatc
     3001 actgcactga ttggtccttc cggctgcgga aaatctacgt atcttcgttc gttgaatcga
     3061 atgaatgatg ggatcgctaa tacgaaaatc actgggaaaa tcatgtatca aggcgtagat
     3121 attaatgcgc ctcaagtgga tgtttatgag atgcgcaaac ggattggcat ggtttttcaa
     3181 cgtcctaatc cgttcagcaa gtcgatttat gacaatatta catttgcttt gaaacagcac
     3241 ggcgaaaaaa acaaaaagaa attagatgaa atcgttgaaa caagcttgaa acaagctgcc
     3301 ttgtgggacc aagtaaaaga cagcttggac aaaagtgcct tggctttatc tggcgggcaa
     3361 cagcaacgtt tatgtattgc tcgtgcgatc gcgatgaaac ctgatatctt gctgctagac
     3421 gagccggcaa gtgctttgga ccctatttcg acaggaacgg ttgaagaaac acttgtaaac
     3481 ttgaaagaag actatacgat catcatcgtg acgcataaca tgcaacaagc agctcgtatc
     3541 agcgactata ctgccttttt ctatttagga aaagtaatgg aatatgacaa aactaacaag
     3601 atatttacca gaccaaaaat tcaggcaacc gaagattatg tgtccggtca ttttggttaa
     3661 gacggaggcc tcaacatgac aaaagagatt atttcatcta aagatcttca tctgtattat
     3721 ggaaagaaag aagcactaaa agggatcgat ctaagcttca atcaagggga aatcactgcg
     3781 atgatcggtc cttctggttg cggaaaatcc acttatctgc gttctttgaa ccggatgaat
     3841 gacctgattc cgaatgtaac gatcaccgga agtgttttat ataaagacaa agacatctat
     3901 ggtccaaaaa cagatgttgt tgaactaaga aaagaaatcg gcatggtatt ccagcagcca
     3961 aacccatttc ctttttctat ctatgaaaat gtcatctatg gattgaaatt aaaaggggaa
     4021 aaagataaaa aagtccttga tcaagtagtt gaagaaagcc tgaaggcagc gtctgtttgg
     4081 gatgatgtga aagacaaatt gcataaaagt gctttatctt tatccggtgg gcagcagcag
     4141 cgtgtatgta tcgcacgtgt tttggcagtt aatccggaaa tcatcttatt ggatgaacca
     4201 acaagtgcgc ttgatcctgt ttctactggg aaaatcgaaa gtatgctgtt agaattaaga
     4261 gagcggtata cgatgatcat cgtgacccat aatatgtctc aagcttcacg tatttcagat
     4321 aagacggcat tctttttaga tggtcacttg atcgaataca atgatacgaa gaagatattc
     4381 atgaatccag aaaaacaaga gacagaagat tatatttcag gtcgatttgg ataaggagga
     4441 aacggaatgc tacgtacaca atttgaagaa gaattgctga atctccataa tcagttctac
     4501 gaaatgggga tgatggtgag cagcgccgtg cacaagtcag ttcgtgcata catcaaacac
     4561 gataaaatct tagcccaaga agttatcgaa aacgatataa atatcaataa catggaaaca
     4621 cgcttggaga agaaaagttt tgaaatgatc gcattgcaac agccagttac aactgatttg
     4681 cgtatgatca ttacggtcat gaaagctagc tcggatttag aacgtatggc cgatcatgcg
     4741 gtgtctgttg ctaaatccac gattcgcgta aaagggcaga cgagaatacc ggaaatcgaa
     4801 aaagagatct ccgatatgtc cgattatgtg aaaaaaatgg tggacaacgt cctagttgct
     4861 tatgtcaaaa cagacgaaaa agacgcacgt acgatcgcca atatggatca aagagtcaat
     4921 gactatttca atcgaatcta taatgcaaca atcaaaaata tgcaggaaaa tcctgagaca
     4981 gtcatcagtg gaacagacta cttgaatgtt gccagttatc tagaacggat cggagactat
     5041 gtgacaaaca tctgtgaatg gatcgtctac ttggctacag ggaaaatcac tgagttaaat
     5101 acgaaccaca atgagatttt ctaaactgaa gctgtgatta tgccgtttgt ttaggaaaag
     5161 aggttgtgaa atcagcggtt gtgaaaaagc tgttctgcat caagtaataa gaacagtcaa
     5221 acaaaaaata gctcctcata tttttcgttt ggctggactt attaccgcag atgcaagctt
     5281 ttgaacaccg tttattcggg aaagggtttg tgacaaaaca tgtcacaaac ccttttcatc
     5341 atttcataga aaggataaag aatgcaaaaa aaaattagca tcatcactat attttttatt
     5401 tgtgtgctta cttcatgggt tattcagcat caagccgctt tatatgaagt acctgtcatc
     5461 gagatagaaa aagtacgaat aaaagaggat catcaattga ttacaggaaa actgatgaac
     5521 acggatcaaa aaggagaatc gatttattta tcggttcctt atcaaaaaaa tcaaatagaa
     5581 acagcaaata taaaagcgag acaaatgtat ctagtccaaa tgcatggaga tgaatggcag
     5641 ctagtagcaa aaaaaaatga tggctggctt ttcttcttca caagtatctt cattggcgcc
     5701 atgttgctta ttggtggaaa acagggtcta tttgctttag gaagtctcat catcaatatc
     5761 gttttacttt tacttttgtt gctcatcttt actaaaacag aagggatttc tctattaaat
     5821 atatctatcc tgttcgtcgt tatagggatg attattagca tattggcttt agatggatgg
     5881 aatagaagca gtataatcaa aatttcagct gcagttacga gtgttttttt agcatttttt
     5941 atttgtcttt tgacaatgaa tcattggaaa gaccaaggac tgcgctatga ggaactgaat
     6001 tatctgacac gaccgtatcg ttctgttttt ctttctagcg tactgattgg aacagctgga
     6061 ggaacactcg ataccgttat cacagtcatt gcgacattag aagaattgac taagcgacaa
     6121 ccagaaataa cagcgaaaca gttatggctt tcaggaaggc aagtgggtcg agatgtgatt
     6181 ggctcgatga ccaatatcct tttgttcgtc tatatcagtg ggtcagttcc atcgcttctt
     6241 ttatatttag gaaatcaatg gtcgttcaaa gaaacgatag aacagcattt atcattggaa
     6301 tttttacgcg tcattgctgg cagcttagga atagtattat cgatccctat ctcggtcctc
     6361 ttttttctga ctgtaaggag gctgaaacga tgaatgtact tattatactt gttgggattt
     6421 tagcaatcag cgtgctactt attggcggta cgcaaggtat gtatattctt ttaggcttat
     6481 tcatcaattt agggatcttt ttcctgttgt tattcggtta tcatcagaag tggccgatac
     6541 tggcattgtc ggtcatagga tttttattga ttgctgtggt tatcttattt tttatcaacg
     6601 gttataatct aaaaatgcgt gcagcttttg tgagtatact gatcttttta ttttgtttcc
     6661 tactattgct tcctatcact gattttttag caatccaagg ttttacatcg gtagaactag
     6721 aagaattatc tggcttagat aagactttag cgattgattt tagattactt acgagatcgt
     6781 tgcttctgat tagtcttagt ggtgctgttt tagatgcgag tgtagcaatt tctagtggga
     6841 cattcgaagt atatcaggca aatccgcaat tatcttttta ccaattgcgc cattctagtt
     6901 tcgctattgc taaaaaaata ttggcgtcaa cagtaatgac acttcttttt gcattcatgg
     6961 gaagttcttt agcgttgatc ctctggttta ttgatttaga cattcctttt cagcaagtga
     7021 tcaatgaaaa atcatttgtt ctagaatata cgatggctat tcttaccaca ttgtccgcac
     7081 tacttgttct tcctttaacc tgcgtgacta cagcatatct ctttacaaaa aagaaagaac
     7141 aactcaagat ggagtaaaag ttacaactta agtcctatga catttttact gaatcagtgc
     7201 ataatatgga tgtaaggaaa agcttgcaaa caattttgct taaggaggca atttccatga
     7261 aagaaagaga acgtatttta gatttagtga aaaaaggaat cctttcgact gaagaaggct
     7321 tagatttatt agaaagcatg gcaactgaaa aagacgaaaa gcaaatcaaa aaagaagcag
     7381 acaaggtaac tgctagtcat aaagaaaaag atcaggcaag tcaattgatc gataaacttg
     7441 aaaacggcga agaagaaatt tctgagccag ttgatccaaa ggaaagagaa agacaagatc
     7501 aagaaaactt agaaaagatc ttagatgaac tagcaactga agcgaataaa acttcggcgc
     7561 gtctagatga agtaaatgct caattcgctg acaataaggc agcacgcaat gagaaacagg
     7621 aagcattgat gcagctgaat acaaaagaag aattaggcga gttgacagaa gaagaattag
     7681 cacagcgtca gacgttagaa gcagaaatca aagaattaga agctagtggc gatacactgt
     7741 tagaagaaca attgaaatta gaagcagaat taaaagatat ccgtaagaat caatggtctg
     7801 agaaaaaaga aacgtttact gataagtttg aattgccaga tgattggaaa gatcaagcaa
     7861 cagatacact gaatcaagta ggcgaaaaaa tgtcagaagc tggatcacag ttaggcaaat
     7921 tcttgaagaa aacattccaa actgtttcag aaacagtgaa tgacaacatg gaatggaaag
     7981 atgtcagctt acgtgtccct gggattgcga caacgaaatt tgagcatgaa ttttactatg
     8041 aagctcctgc agcaagtatc cttgatatca aggcagcaaa cggaaacgtg acgttaaaaa
     8101 cgtgggacag caacgatgtg aaagttgaag ctaagatcaa actttacgga aaaatgggtg
     8161 ctgaaccatt tgaagcattc tctgaacgga gccagattga ggttaatgaa gatcatatct
     8221 ccttccaaat tccaaataaa cgtgtgcgtg cagaccttgt tttctactta cctaaacgag
     8281 tatacgacca cgcagctatt aaacttctaa atggcaacat catgattgaa acattggaag
     8341 ctaaagatat ttatacaaaa tctacgaatg gtaacatcat cgtggaccaa ttaactgcaa
     8401 cgatgctgga agtagaaggt gtaaacggca atatcgatat tcgaaacggg aacatcttag
     8461 attctattat cgaaacagta aatggtacag tgacatttgg tgcgacagca gaaaaccttt
     8521 ctgtgtcttt ggtaaatggt gatgtacgtc ttacgattaa agaagataat ttgaaaaaag
     8581 tagaagcaag ttcagtgaac ggaaatgtca aagtcgcttt accagatggc atcggtttgg
     8641 aaggacatgc taaaacaagt cttggaagta tcaacagccg tctgtccaat tacgaagtag
     8701 ttcgtgagaa aaaagaacgg acaaaccaaa tgttgcaatt ccgtcgtgtt tcagatggtg
     8761 aaatcgcgca agtgcaactt tcaacgacaa caggaagtat ctatttaaaa gatacagata
     8821 aataaagagg ttgtgaaaga gatgttcagc cttgagcaat aagaagcgat tttgtaaaat
     8881 agcttcccct attttacaaa attgtgcctt attgccgata gtctatctct tgaacaccgt
     8941 ttaccaagag attatgaacc tgccaattat cgccgagtca caaggaataa tttcagaaaa
     9001 agctcgtcac gttttatgaa tatggtaatt tgtgaccgag aattttgcag aaagacaccg
     9061 attaagaaga aggttgtcac aacgattttc ttcatttaaa atagtagaac agaggtgaga
     9121 catgatgaaa aaaaagttga caaaatctaa taaaaatgtt gttttgacag ggactttagc
     9181 aggaatagga gaatacatcg ggattgatcc gactgttatc cgtgtgcttt atgtattcct
     9241 gagcttagtt gcttttggaa gtccaattat tttatacatt ttattagctt tgattatccc
     9301 atttgcgcgt tctgcacgaa caaactatgg acataacaat gcatattatg aaaaaaacaa
     9361 ttatcgtgaa caaactcgtg aacaaacaaa aaaacgcaaa gaagcggaaa aaatcgatga
     9421 tgacgaatgg agtgactttt aatgtcgtac tttcaacgta ttttagttaa tacgttaaca
     9481 ttcatatcat tggctgttct ttttcctgcg atggtccatg tgagcagtat ttggacggcc
     9541 gttcttgcca gcttcgtatt gtctgtgttg aatatgtttg tcaaaccgat tttgacgata
     9601 ctgtccctgc cctttacatt agtcacattg ggattgttta cgtttgttat caatgcagcg
     9661 attcttaaaa tgacctcctt ttttgtggga gcagaacatt ttagtttttc ttcattcggt
     9721 gcagcgttgt tagtagctat cttgatgtcg cttgtcaatg taattgtcag cgaacataac
     9781 agtagaaaat aaaagaaagc tgtgccgaac tatcggcgca gtttttttat ttagaatcag
     9841 gaatgcgttt tctattacat tggtaattaa ataaaaacgt tttttttaat tggtagtact
     9901 tttggctaga caatgataaa atgatagaga acactctaat agaaatgagg tagcatgaat
     9961 gtcagaagtt gtaaaagtta gccaattagt gaaaaaatta tctttggaaa tcgtgacagg
    10021 cgacgagaaa agcttagacc gtgtcatcgt aactggggac atctcacgcc caggactaga
    10081 gttgactggt tattttaatt attattccca taatcgttta cagctatttg gcagtaaaga
    10141 aatcagtttt gcggaacgga tgatcccaga agagcgtttg atcatcatga gaagattatg
    10201 tagtgaagat atgcccgcat ttattatttc aagaggatta ccagctccag aagaaatgat
    10261 ccaggcagca aataaaaatg ggatagctgt actgcgttct cccatctcaa cttctcgttt
    10321 attaggagaa ttatccagtt atttagatgg tcgccttgcc cctcgaacaa gtgtccacgg
    10381 tgttttagtg gatgtttacg gattaggtgt actgattcaa ggtgacagtg ggataggaaa
    10441 aagtgaaaca gcgttggagt tgatcaaacg cggccatcga ttgatcgcag acgatcgagt
    10501 agatgtttat cagcaagatg aattaacggt gatcggagaa ccgcctaaaa tcctggaaca
    10561 tctgattgaa atccgaggaa tcgggatcat tgatgtaatg aatctttttg gtgcgagtgc
    10621 ggtacgtggc tctatgcaag tccagttggc tgtttatctg gaagcatggg caaaagataa
    10681 gaaatacgac cgtctgggct ctgaagacac gaccgttgaa atcgcggaag tgggcattcc
    10741 acaaataaaa atccctgtca aaacaggacg aaacgttgca atcatcattg aagtagccgc
    10801 gatgaatttc cgtgcacgta caatgggctt tgacgcaacg aaaacatttg aagaaagatt
    10861 aagtcgcttg attgaagaaa actcaggcaa cgactagcaa ggagtgacaa tatgttagca
    10921 caaatcaatc gagttgcttt tgagcttgta gggattccta tctactggta tgcattgatc
    10981 atcgtatcag gtatcattat cgctatgtgg cttagcagca gagaggctgt tcgggtagga
    11041 ttaaaagcag atgatgtgac agattttatg ttgtggggat tgcctatctc cattattggc
    11101 gctcgcttat attatgtttt atttgatctt ccccagtata tagctgatcc aatccaaata
    11161 ttcaatatcc gttcaggagg cttagctatt tatggcggac tgattgcagg cgcgatcacg
    11221 ctgtattttt ttactaaata tcattttatt tctttgtgga cttttttaga tatcgctgca
    11281 ccaagtgtat tattagcaca ggcaatcggt cgttggggaa atttcatgaa ccacgaagca
    11341 tatggaccag acacgacgcg aagcttctta gaaagtctgc atttaccgca attcatcatt
    11401 gataatatgt atatcgaagg gatctatcgt caaccaacat ttctttatga atcactttgg
    11461 agtttgattg gatttatttt gcttttgttt ttacgaaaga aaccacacct tttgaaaaaa
    11521 ggcgaagtcc ttttatgtta tctaatgtgg tatagtttcg ggcgtttctt tattgaagga
    11581 ttacgtacag acagtcttta ctttttaggt gtgatccgta tttctcaatt attatctgct
    11641 gtgttatttg tcggtacatt gattctattt atttggcgca gaaaaaaagg aaatttaccg
    11701 gattatgacc gctcattagg aaaaaatcaa caaattatct gatttttaga gcgttaaaaa
    11761 tgaaaaaaat ttcaggagtg gtaaaatgaa ttatagagta gcggttttag gaccaggctc
    11821 ttggggtact gctttggcac aagtcctcgc cgaaaacgga catgacgttc ggatttgggg
    11881 acatcgtgct tcacaagtag aagaaatcaa cacccaacac acgaatcaac gttatttacc
    11941 agatcattat ctacccgatt ccatccaagc attcaccaat atggaagatg cagtaaaaga
    12001 cgcagatgcg gtgctattcg tcattccaac gaaagcaata cgttctgttg caaaagaact
    12061 agtgcctaaa ttgaatacaa agccagtgat catccatgca agtaaaggat tagaacaggg
    12121 aacacataaa aggatctctg acatcttgtt agaggaaatc ccagcagaaa aaagaaaagc
    12181 tgttgttgtt ctttctggac caagccatgc agaagaagta gcaaaacatg acatcactac
    12241 gatcacagct gcaagtacag atgcaaaagc agcaaaatat gtacaagagc tgttcatgaa
    12301 taattatttt cggatctata ccaatcctga tgtgatcggt gtagagatgg gggcagcttt
    12361 gaaaaatatt attgctattg gtgctggcgc tattcacggc ttagggtttg gcgataatgc
    12421 aaaggcagca atcatgacgc gcggattagc tgagatcagc cgtttaggtg tagcaatggg
    12481 tgccaacccg ctgaccttta tcggattaag cggaataggc gatctagtcg ttacttgcac
    12541 aagtgttcat tctaggaact ggcgcgcagg caacttgtta ggacaagggc agcccctaga
    12601 agaggtgcta gccaatatgg gaatggtcgt tgaaggtgtg aatactaccc aagcagcgat
    12661 ggaactagcc caaatgatgg aaatcgaaat gcccatcact caaacaatct atgaagtgct
    12721 gtatgaacaa aaagaaatca aaacagccgc aaaagaaatc atgttacgtg atggaaaaaa
    12781 agaaaatgaa ttctcattaa caattttttg aaggagcgaa tgtatatgaa agttaaaaaa
    12841 gcagttattc cagcagcagg attaggaaca agatttttac cagcaacaaa agctatggca
    12901 aaggaaatgt tgccaattgt tgataaacca acaatccagt tcatcgttga agaagcgttg
    12961 aaatcaggta tcgaagacat cttgatcgtt acaggaaaag ctaaacgacc aatcgaagat
    13021 cactttgatt caaatattga attagaaaca aacctaagtg aaaaaggaaa gaccgaatta
    13081 ttgaaattag tagaagaaac gacagatgtc aacttgcatt tcatccgtca atctcatcca
    13141 aagggtttag gacatgccgt tcttcaagca agagccttta ttggaaatga accatttgtc
    13201 gttatgctag gcgatgatat aatggaagac gaagtaccat tgacacagca attaatcgat
    13261 gattacgaac gtactcatgc ttctacgatc gccgttatgc aagttcctca tgatgaaact
    13321 tcaaaatacg gaattatcaa tccaggagaa gtattggaag atgggttata caatgtcaaa
    13381 aactttgttg aaaaaccaga tccaagcaaa gcaccaagtg atttagctat tattggacgt
    13441 tacttattga ctccagaaat ttttgatgta ttgtccgagc aacaaccagg agccggaaat
    13501 gaaatccaac taactgacgc aatcgataca ttgaataaaa cacaacgcgt atttgcccgt
    13561 aaattcacag gtaaacgtta cgatgtcggt gacaaatttg gttttatgaa aacaagtatt
    13621 gaatatggtc taacacatcc agaagtaggt gcacctttac gtgagtacat cctgtcttta
    13681 ggtaaaacat tagaagcaga aggaagcaag atcgatcata atccaaaagg aataacagaa
    13741 atcaaagaag aaaagaaaaa ataacccttt tttctaacga gcttcagctg ctaattgaat
    13801 aaaaagtagt tgaagctcgt ttttatttgt ttaaaaaaca cgaaaatgct atccttatag
    13861 aaaagagaga caagaaggga gtttgatcat gtcaggagga gaaattgcag gattgatcgc
    13921 agcaatcgcc tttgcgatac ttgtcgtttt tatcgttcgt gtgttgttgc aagtttctaa
    13981 aactttagaa aaagtagatc agactgtagc agaagcaaat acgacgatcg aaatcgtcac
    14041 aaaagacgta gatcttttgt ctagacaagt agaaggctta ctggtcaaaa gtaatgaact
    14101 gctcttggat atcaatggaa aagtcgcaac gatcgatcca ttgttcacag cagttgctga
    14161 tttaagtgaa agtgtatcag aacttaatca ttctagtaaa aacatcgcga cgaaagtcgg
    14221 cggaatcggt aaaacgacag ctaaagcgac ggttgccgga aaagtcggtg ggacagcgat
    14281 gaaatttttt aaaaacaaaa aacatactga aacgacagga ggaaaataaa aatggctaaa
    14341 aaaggtggat ttttattagg tgcagtagtc ggaggcacag ccgcagcaat cgccgcgtta
    14401 ttgttagcac caaaatctgg taaagaactt cgggatgaat tagctgccca agcggatgat
    14461 ttgatggata tggcttcaga ttacacagat atggcgaaag aaaaatcgac tgagttaaca
    14521 ggtattgcaa aagaaaaagc gcaagatctt tctcaacaag caggtgatct agcaggtagt
    14581 gtcaaacaaa aagtaagtga atcagcagat gaagcaggaa gtgaatctga cgatatcttg
    14641 ttaaatctta aagaacaatc agctgactta tctaatcgtt tcaaaaaaac ggcagaagat
    14701 atgaaaaaac ctgcagatga attaggaagt attgctaaag atacttcaga agatatcatg
    14761 attgatgtaa aagacgctgc tggtaaagca ggagatactg tcaatgaagg aatgtcagaa
    14821 gcaaaaccag ttgcagatga ctcaaaagaa atgatgaaaa tggcaaaaga agatgcgaaa
    14881 gaagctaaaa aagaaataaa agatacaatg acagattcat ctacaaatca ctaaaatcac
    14941 aaatttatag aataaaaagg tgggagcgga ctagctctca ccttttttac tcttattttt
    15001 ctaaaacaag taattctttt ggtgtagtcg tgaatggcac acagccatcg cttgtcacat
    15061 atacacagtc ttcgatacgt acccctactt tttcaggaat atagattcct ggttcaagag
    15121 agaagcacat cccttcttcg attactaagt catttccttc tacaagggaa gggaattcgt
    15181 ggacggttgt tccgatcccg tgacctaaac ggtgattgaa atattcgccg tagccatagc
    15241 tgttgatcac gtttcgtgcg atttgatcaa gttcacctgc tgtcacacct ggacggacgg
    15301 cttcttgtgc ggcaagctgt gcttcaagag tgatgttgta gattttttct tggaagtcgg
    15361 ttggtttttg ataagcaatt gtacgagtag catcactgca atagccattc caaacaacgc
    15421 caagatcgaa taacacaagt tcattcggtg aaatcttcgt atttcctggt gtaccatgag
    15481 ggcttgcagc atttgctcct gcaaggacta atgtgtcgaa agacatttgt gaaacacctt
    15541 gttttttcaa ttggtattca atttctgcaa tgatcgcttg ttcagtcacg ccttctttca
    15601 ctgctttaaa gccaatctca aatgcaacat cggcccattt accagcttcg atcagtttgt
    15661 ctttttcctc tagagttttg atcagttgta gacgttggat gatggaagtt agatcagtgt
    15721 aagaatccgt tcccgtcaag atatcagaga tagcatcgaa gcgctcgaca gtcaaggcgt
    15781 tcttttcaat ccctacattg ccaggctgac catagcgagc acgaagttct tttccgatga
    15841 tctcccaagg gttttcgctg tctaaatagc cacggacatc atacgcccaa ctactgtttt
    15901 gagcatcttc aacttccaaa gcaggagtga aaaggaaagg atcttgttca agagggataa
    15961 aaagagctag tacacgctca tgaggatcac tttcgtatcc ggaaaagtaa gcgatgtggc
    16021 cagggtcgct gatataagcc aattcgactt ggtgttgtgc catccattga cgtagttcat
    16081 tgatttttac gttattcatt tggcaaccta ctttctaaat taattttgta ttcaataatt
    16141 gtaccactga tttattaaaa gaccaacgtt ctaaaaactg aaaaaagtaa aaattttcat
    16201 gaaaacagag ataaaacggt tgcaattctt gagattattt gatattctat tttagaaatg
    16261 taagacgtat tttcagaaaa catataaaat atgaatagga gaacacaaat ggaaaagcaa
    16321 acaatcacta tctatgatgt agcgagagag gcaaatgttt cgatggctac tgtttcacgt
    16381 gtcgtaaatg gcaatccaaa cgttaaacca gcaacaagaa aaaaagtctt ggaagttatc
    16441 gatcgcttgg attatcgtcc aaacgcagtt gcgcgtggct tggcaagtaa gaaaacaaca
    16501 acagttggtg taatcatccc tgatgtcagc aatatgttct ttgcttcttt agcacgtggt
    16561 atcgatgatg tggcaacaat gtacaaatac aatattatct tagcaaattc agatggcaac
    16621 gaccaaaaag aagtcaatgt attgaataac ttactggcta agcaagtgga cggcgtgatc
    16681 ttcatgggtc accatatcac agacgaaatc cgtggagaat tttcacgttc aaaaactcct
    16741 gttgtattag ctggctctat tgatccagat gaacaagtag gtagcgtaaa cattgattat
    16801 gtatccgcaa caaaagatgc agtaaacaaa ttggcaaaaa gcggcaataa aaaaatcgct
    16861 tttgtcagtg gtgcgttgat cgatcccatc aatggacaaa atcgtctgaa aggctataaa
    16921 gaagcattaa gcgaaaataa cctaccatat agcgaaggat tgatctttga agcacaatac
    16981 aacttcaaag atggtctatc tctagtcgat cggatccata atagcggtgc aacagctgtc
    17041 tatgtttcag atgatgaatt agccatcggt attttggatg gtttattaga ccgtggcgtg
    17101 aaagtaccgg aagaattcga aatcatcaca agtaacaatt cactactgac agaagtagct
    17161 cgtcctcgtt taacaagtat cacacaacca ttatacgatt taggggcggt atctatgcgc
    17221 ctattgacga aaatgatgaa taaagaagaa gtcgaagaaa aaacgattat cttgccttat
    17281 ggaatcgaag aaaaaggatc tacaaaataa taaaaggaca accattcaga ggttgtgtgc
    17341 ctgccgttta actctgcgtt acaaggagca atttcaaaaa acagcatctc atgtttgatg
    17401 aatattgtga ctcgtgattc ggagttggca ggagaacacc gtttatttgg gaaaaggggt
    17461 tatgacaaaa ttttgttata atcctttttt tgtgtcttaa aaaagagaga aagtggttga
    17521 aattacattt atcgtatata aaacaatccg tgtatagatg ctgcattttg ttcaccacca
    17581 tcaaaaaaat aagggactgt gacaatacta ttgtcacaat cccttattac tgtgtattct
    17641 attaaatcaa tcatctttct catctgattc tttagttgct tgattcgctc ttgcataagc
    17701 tgaagcggtg ttccagtaat ctgtatagtt ttcatcggta ccgccaacgc caaatttaaa
    17761 ggcgcttttc ttcggtccat ttttcgccca tagcgattca actgtttttc ctggtgtttg
    17821 gatggatttt ttattgacag taacttttcc aggtgtttga ccagtaaatc cagatacttt
    17881 tttcttcatc acatcagatg ataattcgaa tttctcatca gtgccaaaaa tgtcaggttt
    17941 tgcttggtaa gcttgattga tcagattggc aagataatta gccgaacgta tccttgcttt
    18001 gctgtccatt ccagtattgt catcgtgccc tgcccagcta ctgatggtaa tagacggagt
    18061 agagacaatt agccatgaat ctcggtattc attggtagaa ccagtttttc cgacccaatc
    18121 tgcttttcct agatttccgt ttaagcttga gatggcatct ttgaatggtg tagtgatttt
    18181 tgcattgatc acactacgca tcatgtcatt catgatcgat gccgttgctt ccgaatagat
    18241 ccggacagag ttgctttcgt gtttataaat tacatttcct gcgttatctg tgatggaatc
    18301 gatgatatag ccttcttcat agacgccgcc gtttgctaaa gcctgaaatc cgttggtctg
    18361 ttgaagcgtc gtgacatcta ctgtacctaa aggagcagat tcaacgcccc aattgtcatt
    18421 tgccgggtag ttcatctttg ccaagtagtg ctcgtaagca tattgtttcg atccaccgga
    18481 attcaacaca tcttgatata agtgataagc cgggatattg ttggaccatt ctagtgactc
    18541 acggacggtt tggaaggtgt tcgatccttc gttagtagca ttgacgattt tctcgccagc
    18601 attttctcct tcttgccaag ttgttgcata atctgataca cgggattctg atccgataag
    18661 ccccatgtcg attgcaggtc cataaacaag aactggtttg attgatgagc cagcttgacg
    18721 ttctgtatcg aaggcatggt tattttgatt ctgactgtaa ttccgtccgc caacgaatcc
    18781 gtagatgcgg ccagttctgt tatccatcaa tacatttccg gtttctactt gactgccagc
    18841 accatcatct aacatgtatc cataattttc aacgccattt tgcaatgcag catagacatc
    18901 tttatcgatt gtcgaatgga ttgtatatcc tttattttgg atcgtttgct gtgccttttg
    18961 ataataagcg tcactgaccg atgaatcaga catatcagcg ttgtcttttt cagccaattg
    19021 ttgtgcaatg attcttgttg cttcattcat cacagtataa tacaagaatt ctctgtcagt
    19081 ctgttctgcg atttgttgtg gtagaaagtc ttttgtaagg tcataggctt ttgcttcttc
    19141 gtattgttct ttcgtaattt ggccttcacg atacatactg aataaaacaa aatcttttct
    19201 ggcaaggccg gcagaaagat cttcttttag cgcgcctgtg ttcgtgtatg gactatacgt
    19261 gatcgggctt tggggtaatc cagcgatata agcagcttgc ggcagcgtca catcttttgc
    19321 atctacacca aagattcctt ttgcagcttc ctggactcca gcaatgtttt gtcccttatt
    19381 attacgacca aatggtgaga cattcaaata cgtagcaatg atctcatctt ttgaaaagta
    19441 tttttctact tgtgcagaaa gaatgatctc gtttgcttta cgtttgaatg ttgtttcatc
    19501 tgtaaggatc tgctgtttga ctaattgctg tgtcaaagtt gatccaccag aagagccaat
    19561 accagtagct tcagatacta atgctcgtaa aacagcttta ggaacgtaac cttggtgttt
    19621 atcgaagtat tcatcctctg tgctgataat tgcagttttc aataaaggtg aaatcttatc
    19681 agatgaaata gtcgtgcgca tcagatctgt ctggattttt gatatctctg tattatctgc
    19741 ataaacaatc tttgacgttt ctgtaatgtt tcctagttct gtctgcagtg cttttttagt
    19801 cggcaattgt gtgtcttcga cgagataggc aaaatagcca gcgcctattc caatacctag
    19861 agctgcgaac atcaaaagaa gaacagtaat aaaaacagtc aatgattgaa aaactctaaa
    19921 aataatctta ggaacaaacc atttttcttt ttttcttcta ttctgttttt ttcgagtatt
    19981 tttgcgtggc aaaagggtca cctcaactaa tggttttatt tcgctaatta tagcaaataa
    20041 tagaacaaaa aaacagacaa ggctgttttt ggcaggaatt tcttttttaa aagagttaaa
    20101 acgtgagaaa atgatcttta ttataaaaga gttcttaagc ttttctttag agaaaatttc
    20161 agttactatg gagaacaggg caaagaaaaa tcaatcattt tctttgtata tcatcttacc
    20221 aatatatagg aattgttagt aacaattgcc tttcaaggag gaatccatcc atgttattag
    20281 gctcacacgt cagcatgagc ggaaagaaaa tgctgttagg agcagcagca gaagcagcca
    20341 gttatggatc ttcgactttt atgatctata caggcgcccc tcaaaacact agaagaaaag
    20401 ccatcgaaga gatgaatatt gatgccggaa aagcgtacat ggcagaaaac ggtctaacaa
    20461 atatcgtcgt tcatgcccct tatatcatta atttggggaa tacgatcaaa ccagaaatgt
    20521 ttccttttgc cgtccaattt ctgagagaag agattgttcg tgcggaagct ttaggcgcta
    20581 ctcaaattac acttcaccca ggtgctcatg taggggcagg aacagaagcc ggaattgcta
    20641 gaattattga aggcttgaat gaagttttga caaaagaaca gacggcacaa atcgctttgg
    20701 agacaatggc aggtaaaggt acggaaatcg gtcgaacatt tgaggaatta gcgcaaatca
    20761 tcgatggagt aaaattaaat gaaaaattat ccgttacttt tgacacctgt catacgaatg
    20821 atgccggtta taatattaaa gaagactttt caggtgtaat ggaagagttt gataagatca
    20881 tcgggttaga tcggcttaaa gtactccata tcaatgattc taaaaatccg caaggttcgc
    20941 ataaagaccg gcacgccaat attggttttg gaacaatcgg attcgacacg ctgaataaga
    21001 tcgtccatga tccaatgttt gacaatattt cgaaaatctt ggaaactccc tatgtcggca
    21061 aagataaaaa gaaagcttac gcaccatatg gaaaagaaat cgccatgttc aaagcacaaa
    21121 tattcgatcc agaagtgttt cctgagttat tagcagaaga agtgatactc taagattttc
    21181 tatctgacag cagcaaaaaa ggaccaggca cattgccttg tccttttttg tttaagaaac
    21241 aaaggagtac tttttgcagt ctttttcatc atgcatcttc tcccccctct ttttaaattt
    21301 agacaaacaa ggtatactgt tagaaaagta aaaaaacgaa aagtttcaca agcaagtggc
    21361 aaaacgtgat tggaaggacg aaaagatgct gaacacttta aaacccttga tcaaccaact
    21421 aaaaaagaaa catacatact ttattcttgg catagcccta ttatttacaa ttattcgttt
    21481 gtatttatat agtaaagcaa tctatggaac agacatgtcg gcaacctatg atgaccagct
    21541 gctgattcgc tatgctcaaa atattttgga cggtaaatgg ctaggggagt atacgactac
    21601 aacactttcc aaaggaatct cctacagctt atttttagta cttgcacata aactgcatct
    21661 ttcttatgct gttttgttcg gcatgttcag tatttttgcc agtatattgg ttgcactcgc
    21721 gtttaaaccg atcgtgaaga acaagtatct tcttttaagt atctattttt attttttgtt
    21781 ttctccgatt agttttacac atgaatattc cactcgaaca tatcgtaatg ccctcgtgat
    21841 tccggctgtt gagatcatta tcgcttgttt actagctatt tattaccgca aatacggttc
    21901 tagcagaaaa ttgctgccat ggtttttagg gctttctgtc gcttttcctt tcttttggtt
    21961 cattcgcgaa gattctatct ggcttcttcc ttttactgta gtagctttgg gtatcagttt
    22021 cgtccagatc ttgctagggc aagacttttc tttcaaagaa gtaaagaagt taaaaaaacg
    22081 actctttctt ctctcgaaac gggaatggct gaaactttca ttgtttttac ttcctatagt
    22141 tggcttttcc atcgtctatc agtggatcga atataaaaat gagacaacat acggtattgc
    22201 aaccatcaat gaccgttcca gtggagaatt tggacgtttg atgaaacaac tgatccgaat
    22261 agatgatgaa acgaacttga ataaagaaaa cagtgagata tgggtctcac atgctgcact
    22321 aaataaagca ttagccgcat ctcctacatt ccatactgtt tctacccgta ttgacgaatt
    22381 atatacgact cacgcgtgga ctcaaggtgg aaaagtaaaa gaattacccg gagatatcat
    22441 cttttgggca ttgcgagatg tggtaagcga atctggttat taccaaaaca atgcaaaaga
    22501 aaccaatgat ttttggaaaa aagtcaacca agaattagaa gaagcctatc acaagggaac
    22561 catcaaaaag aaaaaagagt tctatctgat gggatcagga gatggaaaga cgatgagtga
    22621 tctgccaatc gtattcgatt tccttcaatc aggatacgct tatgcatggc gatataaaga
    22681 tttccatcaa ggaggaaatt atagtttcgg aacgcaagaa caagtcaaac aggctcaaga
    22741 attattaaac cttccgctaa tggagaattg gatcgatagt aatgaaccga gaacacctaa
    22801 tttccaatta acaaaatcag caaaagtcgc gaatgtcatc ataaaaattt accaagttct
    22861 ggctattccc gtgctgattg cttcaatagc agggatatta cttatgatac taggaaccgt
    22921 atttgcaaaa gctgatcgtg cctattatag ttcctctttg attgtggtca ccggattaat
    22981 actgacacaa ttggtttttc ttttcggtgt ctcgtggttt tgttcttatg cgccagaaaa
    23041 aaaggattat tttttaagtg tctacactgg cgcaggcgtg ccattaacgc aattcatcgt
    23101 agtaacaagc atgctagttt tcagtaaatt aaatctgaaa aaggctttat cttctaaata
    23161 aaaagaccct tggtagctag atttttaacc taactatcaa gggtctttgt gtggaaaaat
    23221 cagtttctat tcgatttctt ctaccgtttc ctgcaaacgc tgttcatatt ttttgtttcc
    23281 tttatataaa tcaccaatcg tccatgccaa caggagaagg acaagaacaa tgattacata
    23341 agcgatgtga tcagccaatg ctagttgagc agaagtggct tgttcaccaa agaaactttc
    23401 gatctgtcct ggtaatcctc gtaagttcaa gtaagttaga ctaatcacag agaaccagcc
    23461 aagaagtttt acgatgaatg aatttttaaa tcggctaccc atctccgctt ggctgttggt
    23521 catcatcagt aaagggatca tcgaaaacgg caacgcaaaa gccaagaaca cctgggattc
    23581 attcatcaag ttattcaaag cggtatgttc ttcgatcgtt ccttttgtac ttgtaaacaa
    23641 aacacaaatc aatacaggaa tcacagaaag caatcgtgtg accagtcttc ttagccaaat
    23701 cgggatacgc atatggatga agccttccat gatgacttga ccggtcaacg taccagtaat
    23761 agtagagttc tggccagaag caagaagtgc tacagcaaat aaagtggata agataccaga
    23821 ttttgctaca gcaatcaaga taccattgct catagctgat ggatcagata aggcttcata
    23881 cagaccaaaa aaggaaggat cttggacaga accggttttg aagacagcga cacccatgat
    23941 caatagcagt gcattgacta gaaaagccat cgacaattgg atattagagt cccaagtaga
    24001 aaaacgtaca gcttgtgcta catcttcttc gtcttggtga tcaatcttcc gagtttgaga
    24061 aactgctgaa tgaagatata agttatgcgg catgactgtt gcaccaataa tccctaaagc
    24121 tccagtcagc ggagtttcgc cgccgatgac cggtgagtct gagaatgtat gactgttagg
    24181 gataaagcca gcaaataaac tgtgccaatc tgggttagac aagccaactt gatagacaaa
    24241 aacgaataaa atgacgaaaa ttaagcagac aaccagtgct tcgatcttac gaaacccgac
    24301 ttttgtcagt agtaagagaa cgaaaacatc taatacagta ataaagacgg caatcaccaa
    24361 tggaatatca aaaagtaagt agagggcgat tgctgcacca atgacttctg cgatgtctgt
    24421 tgccataatg gctaattcag tcaagatcca taaaatgaca cccaatgtct tacttgtgcg
    24481 tgctcggatt gcttgggcca aatccatttg ggtcacgatt cctaattttg ctgccatata
    24541 ctgtaaaagc atagcaatca gactagacat caaaataact gacatcaata aatattggaa
    24601 attttgtcca ccagtgatcg aagtggacca gttacctgga tccatataac caactgctac
    24661 taatgcacct ggtccagagt aagcaagaag tgttttaaaa aaacttttcc cctttggtac
    24721 atcaactgtt ccatttactt cagctaaaga taaaccattt gtatgttcaa taagtcgatg
    24781 cttatgaaat tgtttgttgg attgattatt caagaaaaaa ccacctttcc tatttatcta
    24841 cgattacatt ctacacctca aaaagaaaaa attaaagaaa aatattcggt atgcctaatt
    24901 tattttcgtg gcgcgtcttg ttagatgacg aaagaaattt tgttctcctc taaaaaaacc
    24961 actatttttt gcatgaaact aaagaatacg cctttctttt ttcaataaac tttagtaaaa
    25021 taagtcattg agagcaattc ggaaatagaa aggagagatt tatatgttat caacaggtat
    25081 tgtcgtactt attgctatta ttgctttgct aatcggcgca gtaggtggct ttttcctagc
    25141 gcgtaaatat atgaaagact atttagaaaa aaatcctccc gttaatgagg acatgttgcg
    25201 atcaatgatg atgtcaatgg gacaaaaacc atctgagaag aaaattcgcc agatgatgca
    25261 gcaaatgaag aaccaaggga aaaaataggt tctgacccaa cgacttttta cgggctgatt
    25321 ttttcagtcc gttttttatt ttgtaatttg ttagactatt cagattattc agcaaaaaga
    25381 caagcacaga caatcctagg ctcttgataa agacaaagga ggagtaaaat gtcgattttt
    25441 agaaagttag gctggttttt tcgtcaggaa aagaaacatt atttaattgg ggttttctcg
    25501 ctattcgcag tggccttggt acagttagtt cctccaaaag tgattgggat catcattgat
    25561 gagattgccg ataaaaattt atcagtaaag atcattttcg ggtggatctt cctattgatt
    25621 atcgctgctt tcttacaata tctttttcgt tatatctggc ggacgaatat ttggggaagt
    25681 gcagctcggt tggagaaaga acttcggcag cagttattca aacattttac aaaaatggac
    25741 aatctctttt accaaaaata tcggactggg gatttgatgg ctcatgcaac gaatgatttg
    25801 aatgcgatcc agaatgtagc aggagctggt atcttgacct ttgcagattc agtgattacg
    25861 ggcggaacaa cgatcattgt gatgattttg tttattgatt ggcgcttaac actgattgcg
    25921 ctgattcctc tgcctttgct ggccgtcact tcacgagtgc tggggtcgaa actccatgat
    25981 gcttttcgtg attcgcaaga agctttttct agtatcaatg ataagacaca agaaagtatc
    26041 actgggatca aagtcattaa aacatttgga caagaaaaag aagatctagc ggattttgaa
    26101 gaaaagattg aagatgcaat tattaaaaac aaacgagtca attttctaga ctccttattt
    26161 gatccattca ttaccttgat tatcggtctg tcttatgttg caacaattat tttaggtgga
    26221 aagtatgtct tggaaggcac gatctcgatc gggcagatgg tctcatttat tagttatatc
    26281 ggtatgcttg tttggccgat gtttgcgatt ggtcgattgt tcaacgtatt ggaaagagga
    26341 aatgcttctt atgatcgcgt caatgaactt ttgcatgaaa aaacgcatat cgtggaacaa
    26401 ctgggtgcag tgcagacccc ggtaaaaggc gagctgaatg tagctatttc ctcttttaat
    26461 tatccgaaag aagaatcacc agcattagaa caaatcaagt ttgcaatcca agaaggagaa
    26521 acattaggta ttgttgggaa aactggtgcg ggaaaaacaa cgatcatcaa attactgatg
    26581 cgtgaatacg atgattatca agggaaaatc caatttggcg gacgttcgat caaagaatat
    26641 tcattgaatg ctttattagg atcaatcggc tatgtgcctc aggatcactt cttgttttcg
    26701 atgaccattc gagataatat ccgtttttct aatccaaaac tttcagaaga agaagtagag
    26761 catgcggctt ctttagcatt tatcaacaat gaaatcaagg aattaccaaa aggttatgat
    26821 accttagttg gagaacgtgg agtctcttta tctggcggac agaaacaaag gatttcgatt
    26881 gctcgagcat tgatcaaaaa tcctgaatta ttaatattag atgatgcact ttctgcagta
    26941 gatgccaaaa cagaagaagc tatcttgcat aatttaaaag aaataagaaa agagaaaaca
    27001 actatcattg cagctcaccg tttaagtagc gtgatgcatg caaaagaaat cattgttgtc
    27061 gatcaaggga aaatcattga acgaggaacc catcatgagc tgcttgcact aggcggctgg
    27121 tatgaacaaa tgtgggagaa acagcaatta gaagcgaaga tagaaggagg cgaggcctga
    27181 tggatgaaaa ttatcaatcg gaatggacaa aatcgattcc acttaaagaa cagaaaaaga
    27241 tcatcaaacg actgatgaag ttcgccaaac cttttagaac aacgttcatc gttgctatct
    27301 tatttgcgtt tgctctgtct gtgatcaatg tcctattgcc aaggatcatc caaactttta
    27361 tggatgatca tctggctaaa cagtcggcta cgacacaagt tattttgttt tttgctggta
    27421 tgtacttatt tggagtccta gtaaaaagta tcatttggtt tttccaatgg tatttgtatt
    27481 ccatggcttc attgaagacc tatcagtata ttagggtaaa attatttgaa aagctgcata
    27541 cactaggtat gcgttatttt gatcagacac cggcaggatc aaccgtttct cgagtgacga
    27601 atgacacaga aacgctattt gaattttggt acgtcttttt gatggtattg actgggatct
    27661 ttgcagtcgt ttcttcattc attgccatgt ttcaaatcaa tggagaaatt gccttgtgga
    27721 ctttggtgtt cttgccgatt ttaggaattg tcatctggta ttaccagaaa tttagttctc
    27781 gtatttaccg aaatatgcgg gaaaaattaa gtcagctgaa tacaaaattg aatgaatcga
    27841 tttccggcat gcgtattatc cagcagtttc gccaagaagc tcgtttagct aaagaatttg
    27901 aacaagtgaa caatgagtat ttagacacgc gctatgcgat gatcaaaacg aattcgattc
    27961 tgttaagtcc gatcatcaat tttctatatg cattggcgat tgcgttggcg ttgactttat
    28021 ttgggatcga tgcgttgcat tctcccgtag aagttggact gatctatgca tttacgactt
    28081 atgtccaggg attttttaat ccaatgtctc aaatgatgga ctttttgagc atcttcacag
    28141 atgggatggt tgctggtagt cgtatcttga agattatgga tacggaagaa tatgcaccac
    28201 aacaaaaccc tggagcagat caaatgatta cttcaggtaa gattgagttt cgcaatgtaa
    28261 gcttttctta tgatggagag cacaatgtat taaacaatat ctcttttgtt gctaatccgg
    28321 gagaaaccgt tgcattagtg ggacacaccg gaagtgggaa aagctcgatc atcaatgtgt
    28381 tgatgcggtt ttacgaattt tatgaaggtc agatattgat tgatgatcat gacattcgag
    28441 aatatccaat ggaagagtta cgtaagaaaa tggggctggt cttgcaagat gctttcttgt
    28501 tttatgggga tatcgcagga aatattcgga tgatgaatcc agctatcaca gacgaacaga
    28561 tacgccatgc ggctgagttt gttcaagcaa acaaattcat cgatgaatta ccaaaaggat
    28621 atcactcaaa agtgatcgaa catggtgcca gctattctag tggacaacga cagctgatca
    28681 cttttgcgcg aacaatcgtg actgatccta aaattctagt attggatgaa gcaacagcaa
    28741 atatcgatac tgaaacagaa ggattgatcc aagaaggatt ggcgaaaatg cgacaaggac
    28801 ggacaacgat tgcgattgcc catcggctgt caacgattcg agatgcagaa ttgattttag
    28861 ttttagacaa aggtcatata gtagaacgcg ggaatcatga aacacttcta gcgcaaaatg
    28921 gtttgtatgc agatatgtac aaactgcaaa gtagtggtat ataaaagatg ttgtgacagt
    28981 agcattttga cctaagcact agagtgaaaa taaaacatag cttcccatat ttttcatctg
    29041 tgagagttag tgtcgtagtt accagattta aaaaagacga cgtcagctag tcagaagtgc
    29101 ctaatagccc ctaagaaagc gttgacaaaa tcgtttgctt tcgataatat aaagaaaacg
    29161 atacaacttg gatagtgatt gcctgatgca agctaaacct gatgagtctc ccatggtttc
    29221 atggacggct ttttgggttt ttctttttgt tttggataca gccctatata tcatggaaag
    29281 gagcaaaaca tgctgattga gaaagtactg aataacaacg tcgtaataac aagggatgaa
    29341 agagatcggg aaatgattgt gatgggaaaa ggattggcat tcaaaaagaa ggtgggtgac
    29401 ccgattcttg actcatgcat cgacaaaaaa tttcagttga caaatgacgg actttcacaa
    29461 cagttccaag aattggcaca agcgattcct cttgagtact tagaaatttc ttgtgaaatc
    29521 attgaatata caaaagaggc tctgcaagtc catttaaatg attctgtgta tatttcattg
    29581 acggatcact tgtatacagc tatcgaacgt caaaaaatgg ggatctttgt gccaaatgta
    29641 ttgctgtatg atatcaaaca actgtttcct aaagagtatg ccattgggaa aaaaacagtg
    29701 aagaaaatct cggataaata ccacatcaag ttatcagaag acgaagccgg atttatcgca
    29761 ttgcatttag tcaatgcaca aggcattcaa agccagcaag acaataccta cctgtttacc
    29821 acaacaatac aggatatttt aaatatcgtc cgctattact ataagattac atttgatgaa
    29881 gattcagtcc acttttttcg attcgtcaca cacctgcgtt tttttctgac acgtgtccta
    29941 gatggagcga aatcagaaga tggagaagtt tctgaggaat tgttccaagt tattcaaaaa
    30001 aaatataagg atacgtttgg ttgcgttgag aaaatagcag actttttaac cggggctttc
    30061 gactaccaga tgtccacgga tgaaaaaatg tatttaacga tccatactgc gcgtttagtg
    30121 catgagaatc gttgtaaata aataaaaaag gaggacaaat taacaagaac gtatgaaata
    30181 agggtagtga cattcagttg gctaaacctt ccactaatta attggaggga atagaaatgg
    30241 gaaaatacca ggaattagca gaaaacatcg taaagaatat cggaggaaaa gaaaatatcc
    30301 gtagcttgac acactgtgtg acgcgacttc gtttcaaact aaaggaggaa aataaagcaa
    30361 aggatgatgt tttgaaaaag atggaaggga tagtcaccat catgaaaagc ggcgggcaat
    30421 atcaagttgt aatcgggaat catgtgccag atgtctatgc agaagtactg acagtagccg
    30481 ggatttccga tgaagcagaa gaagaggttt cttcaagtag taatctgttc aatcgattaa
    30541 tcgacatatt aagtggctgt ttccagcctt ttttaggtgc tttggctgca gcgggaatgg
    30601 tcaaaggatt aaatgctttg ttcttgtttc tcaaatttta tactgaagga tcgggtacgt
    30661 acatcatgct gaatgggata ggggatgcaa ttttttattt catgccagtg atcttagggt
    30721 acacagcagc gaaaaaattt catctgaatc cgatggtggg gctcgtgatt ggtgcagcac
    30781 tttgttatcc gactgtacaa ggagcagcac tacaaagtgg tttccctgat ggcagtcctg
    30841 cgccttatag cttgctgggt cttccggctt atgaaacatt tttgggtatc ccatgggtgg
    30901 gcagcagtta tactagtagc gtcgtacctg ttatcttcat tatcgcattt gccggacaag
    30961 tccaaaaaat tgccaagaaa tggattcctg cagtcgtcca gacctttttg gtttcttttt
    31021 ctgtgttatt aatcgcactt cctgctggct ttttgattat cggtccgatt gtcagtatac
    31081 tgacagatct attaagcacg ggattccaag cattgatgga cttttctcca gctgtgtatg
    31141 gtgcagttct agggtttctt tggcaagtac tggtgatttt cggacttcat tggagtatta
    31201 tccctcttgc gattatgcag ctagcgcaaa atgggtggag tcagatgtta gtaggcgttt
    31261 atggcgcgag ttttgcacag acagcagtgg tattggcgat gtatttcaag ttcaaggatc
    31321 aaaaaatgaa ggcgctttgc atgccagctg tgatttcagg aatcttcggc gtaacagaac
    31381 cggctattta tggaatcacc ttaccgaaga agaagccatt tatttactcg atgattggtg
    31441 gtgctgtttc aggaatctat ttgatggcat ctggaacaat cagctacata atgggcggat
    31501 tagggatttt tggggtggta aactacatca cggaaacaga cgccagtggt atgtttcaag
    31561 cgtttatcgg cattgctatt gcttcggtaa tcgggttttc gctgactttt ttcttttgga
    31621 aggatgaagc aggtgaaaca gatgctatac agaaaaaatc aggtaaagaa attgtaaaaa
    31681 caccgataca aggacaaatc gctccactaa atgcagcgaa ggatgcagct tttgcacaag
    31741 gaacactagg tcgaggaatc ttaatctacc ctgaaaaagg agaagtacgt gccccttttg
    31801 acggaacagt tatgaccctg ttcccgacga aacatgcaat cggaatggtt tctgaaacag
    31861 ggctggaatt gctgattcat gtcggattgg atactgtgca gttggaaggg aaatattttg
    31921 aaagcttagt gcagcaagga gaaagagtga gacaaggcga tctgttgcta cgcttcgaca
    31981 aaaagaaaat cgaaaaggaa ggatacagtt tagaaacacc tgtgatcgtc accaattaca
    32041 cggattattt ggatattttg gaaaaccaag gggagacggt cgggccagac gatgcactta
    32101 tcaccgcact gacctgaacg gttaggtgaa gaatggacta ggcttaacta ttctttttat
    32161 tccacttctt tattttcaaa attgaaaggc tctttcatga tggctgagct gatagggcgt
    32221 ttatcgaatt tttgaccgac agaaattaga tggactaaat tctgttgata attttctgca
    32281 aaaacacaac tatataacac gtctagaaga taacaaattg atgtattgat cgtaaaatta
    32341 ccaatttttg aatacagtcg ctcgcgtgtc gtaagtcgca aagcggctgt actcattgca
    32401 gatagcgttg aatcaccgat actggtcaaa gctaaaattg gaacatcatt ttgtttcaat
    32461 atttctgcgg tacgaaggat cacattgttt tcacccgtat aagaaatcag aatcccgcac
    32521 atatcgctcg tacaattgta cgcttcatag aatgattcac caaaagtggt gacaattgtg
    32581 acatccattt tgatgcgctt cattttcagc atgaaatctt gagaaatcaa agtattcgag
    32641 ttactggcga agatcacgat tttttttgcc tggagcagta aatatttagc tttttgcagt
    32701 tggtcatgat gaatgagaga aagtgtatca tgaatcgtca tctgttctaa agaagccatt
    32761 ttatttgcga ttgtcataat accgtctgtg gtggaaaaag gaagattcgc atctacgtct
    32821 tgaaaattcc cttcaagata ggctgtttca gctaaaaaag cattacgaaa actgacccag
    32881 ccatcaaaac ccattttttt tgctacacgg atgagtgtag acgggtggac ataacatcgc
    32941 tcagctatcg cttggatggt catttcgcca agtgctggag aatcgcttaa gagaaactgg
    33001 atcacttcat tttctgaagg agaaaaggag atttctttga ttttttcacg taataacatg
    33061 tagatacctt cttcttattt ttttacgtaa attataactt ttttgaaaat gttttgtaaa
    33121 cggatttata ctatataact taaaataggc tttttttgac aagatattta caaaaatcca
    33181 ctagaaaaca gtaacttttg taaatgttac tggaaagaaa cggatttcta ttgtttgttt
    33241 tgtttacaaa aaagataatg aaagtgtaaa aaagtaaagg agtgagaaca tggcaaaagg
    33301 aatcaaaatc gctacgattg gtggaggcag cagttacacc cctgagttga tggaaggatt
    33361 tatcaaaaga tatgaggaat taccgatacg agaaatctgg ctcgtagatg tagaagaagg
    33421 aaaagaaaag cttgagatcg ttagtcagat gtctcagcgt atgtgggatg cttctccata
    33481 tgaagtcaag atctatgcaa ctctggatcg ggaagaagca ttgaaagatg cggattttgt
    33541 tacgactcag tttcgtgtcg gtcaattaga tgcacgaatc aaagacgagc ggatccctag
    33601 ttattatggg atgattgggc aagaaacaaa tggtgctggc gggatattca aagcttttcg
    33661 taccgttcca gtcgttttgg caatcgttga ggacatgaag cgtttatgtc caaatgcttg
    33721 gctgatcaat ttcgcgaacc caagtgggat ggtgacagaa gcagttatca gatatggcaa
    33781 atgggataaa gtgattggat tatgcaatgt gccagtgatg gcgatgatga cagaaccagc
    33841 aatgcttggc aagaaaaaag aagagctgat ctacaagttt gccggtctaa atcatttcca
    33901 ttggcacaaa gtagcagata ataaaggaaa tgaccgcacg gcagaattga ttgatcgttt
    33961 atacgatgaa gataatggat taccgaaaaa tatctttgat atcccgtttt tcaaagaaca
    34021 attaaaacaa atgaacatga tcccttgtgg ctaccaccgc tattactatc gtgaagaaga
    34081 aatgctggaa catgctttag aagaatatca aacgattggt acaagagcgc agcaagtgaa
    34141 agaaacagaa gcagagctat ttgaattata caaagatcca gaattagacc ataaacctga
    34201 acaattgcaa aaacgaggtg gcgcttatta ttcagacgct gcctgtgaaa cgattgcttc
    34261 aatctatgga aataaagaaa cacagatcgt tgtatctaca aagaatgatg gagctgttcc
    34321 tgacctgtcg gcagattgtg ttgtagaagt cactgcctat gttggcgcac aaggagcgag
    34381 aaatgtagcg tttggttctc ttcctgctgc tgaaagaggc tggttgcaag taatgaagaa
    34441 tatggaattg ctgacgatcg aagcagctgt gactggtgat tatgggacag ctttacaggc
    34501 gtttacgatc aatccactta tacgatcagg aaagacagca aaacggatca tggatgagtt
    34561 gtttattgca caccagcagt atttaccgca attcaaggaa acaatcagcc gattaaaaga
    34621 agaaggaatc gaagttcaag atgaactggc aagagaatta tcaggtaata cacatataat
    34681 ggaggaagta taatggggtt cagaaaagat tttttatggg gcggtgctac agcagccaat
    34741 caatgtgagg gcgcttatga tgaaggtgga cgcggactag caaacgtaga tgttgtgcca
    34801 atcggaaaag accgttttcc tgttatcacc ggaaagaaaa aaatgtttga atttgatgag
    34861 gatcattttt atccagcaaa aattgcaatc gatatgtatc atcgttataa agaagatatt
    34921 gctttgtttg cagaaatggg gttcaagact tatcgaatgt ctatcgcctg gagtcgtatt
    34981 tttccaatgg gggatgagaa agagccgaat gaagctggct tgaaatttta tgaggatgtc
    35041 tttaaagaat gccaaaagca cggaattgag ccacttgtga cgattacgca ttttgattgt
    35101 ccaatgcatt tgatcaaaga atatggtgcg tggcgttcca gagaactcgt tggtttttat
    35161 gaaaatctgt gtcgagtcat cttcaatcgc tataaaggac ttgtcaaata ttggctgaca
    35221 ttcaatgaga tcaatatgat ccttcatgca ccatttatgg gcgcagggct gtattttgaa
    35281 gaaggagaaa atgaagaaca aatcaaatat caagcggcac atcatgagtt ggttgcaagt
    35341 gccatcgcaa cgaagattgc tcatgaagta gatccggaga acaaagtagg ctgtatgctg
    35401 gcagcaggag catattatcc atacacttgc aatcctgaag atgtttgggc ttctagaaaa
    35461 tcagatcgag aaaattattt cttcattgat gtccagtcac gaggagaata tcccgcttat
    35521 gcattgaaag aaatggagcg aaatggcatt cagatcgaaa tggaaccagg ggatgccgca
    35581 ttgctgaaag aacatactgt tgattttatc tctttttctt attattcttc acgagtttcg
    35641 actaccgaca acgaactctt ggaacagact gcaggaaata tctttgcttc tgtcaaaaat
    35701 ccatatctgc aagcaagtga atgggggtgg caaattgatc cattaggctt gagaataacg
    35761 atgaatgata tttatgatcg ttatcaaaaa ccattattta ttgtagaaaa cggcttaggt
    35821 gcagtcgata cgccagatga aaatggttat gtggaagatg attaccgaat cgattacctg
    35881 gcagcgcata tccaggcgat gaaggatgca gtagaactag acggagtcga tctattaggc
    35941 tatacgactt ggggatgtat cgatttagta tcagctggta ccggtgagat gaagaaaagg
    36001 tacggattca tttatgtcga tcgtgataac caaggaaatg gcacattagc ccgttcaaag
    36061 aaaaaatcat tcaactggta taaaaaagta attgattcaa atggagaaaa tctttcgaac
    36121 aattgatcaa tgatttataa aagtctattg tttttcagcg gatttttcga gtataataaa
    36181 gctattcgag aaacgcaagc agaacaatag actttttttc gcattggctg taaagtatgc
    36241 ttacttgatt aagtaaagga gaatctacat atgtatgatg taatcgttat tggcgcaggt
    36301 cctgctggga tgactgctgc tttgtatgct tcacgttcaa acttatccgt attgatgatc
    36361 gaacaaggtg cccctggcgg ccaaatgaac aatacggcag aaatcgaaaa ttatcctgga
    36421 ttcgattcga tcatgggccc cgatctagct tataagatgt atgaaagtgt ctcaagattt
    36481 gggacagaaa atgcttatgg aatcgttcaa gatatcaagg atcacggcag ccataaagaa
    36541 gtcatctgtg aagataaaag ttacgaagga aaaacagtca ttatcgcaac aggctgtatc
    36601 caccgtaaat taggcgtacc tggcgaggaa gaatttgctg gacgaggcgt ttcttactgt
    36661 gcagtctgtg atggtgcgtt tttccgtaac aaacgtctag tagtcatcgg gggtggggac
    36721 tcagcagtag aagaagccat ctatttgaca caatttgctt ctgaagtagt gatcgtccac
    36781 cgtagagatg aattacgtgc acaaaagatc atccaagacc gtgcatttgc aaatgaaaaa
    36841 atttccttta tgtgggatac agtcgttgac gaaatcgttg gaaacgaaat ggtagtgaca
    36901 ggtgtgaaag cacgtaatgt caaaaccggc aatgaatttg atttagcagc agacggagta
    36961 ttcatctatg ttggactgga tccattaact gcacctttta gagaagcagg tttgacgaac
    37021 gaagctggct ggattccaac agacaacgaa atgaaaacga atatgccagg cgtttttgcc
    37081 atcggagatg ttcgtgaaaa agatttgcga caaatcacta cagctgttgg tgacggcggt
    37141 attgctggac aacaagtgta taaatacttg gagtctttag aagaaacaac agcataagtt
    37201 taaaaagagt gaaagaacgg tttcaattgt tcttttactc ttttttttcg cgtgaaatcc
    37261 actgaatccg atttatgtga atacttgcaa tcatgcttga taaaagaatc ctttcttgat
    37321 ttaagcgttt tttggtcctc atactttcat atctaataag tgaatgctat acttattaag
    37381 aaccatattt cagaaagagg tgttattttc atgtcttggg aacaagtgta tgaacaatgg
    37441 gtacatgaag aaaatttaga agaaaactta aaaaaacaat taaatgatct gggtgaagat
    37501 cctgaaaaac ttgaagatgc tttttatgca ccgttggaat tcggtactgc aggcatgcgg
    37561 ggaatcttag gacctgggat caatcgaatg aacatctata cggtaagaca agcaacagaa
    37621 ggactagcac gtttcatgga tacacaaggt gaagaaacaa aacgccgagg tgtcgctatc
    37681 gcttatgact cacgccatca gtcaccagaa tttgcaatgg aagcagccaa aacactggca
    37741 catcacaata tcccgtctta tgtattcgaa agtctgcgtc caacaccaga gctttctttt
    37801 gcagttcgtc atttgaagac gtttgcggga atcatggtaa ccgcatctca caacccagcg
    37861 gcttacaatg gatataaagt ctacggagaa gatggcggac aaatgcctcc tgcagacgca
    37921 gatgcattga cgaaatatgt ccgtgaagtc agcaatccgc taaaagtcga agtattatct
    37981 gacgaagaag ctaaacatag cgggttgatc acgatcatcg gagaagaagt ggacgctgct
    38041 tatctagaag aaatcaaagc agtgacgatt gatcgtgaat tggttgaaac aatggggaaa
    38101 gatttgaaac ttgtgtacac gccacttcat ggaacaggga aaatgttggg cgaacgtgcg
    38161 ttgaaacaag ctggattcga acaattcgta ctagttcccg aacaagctgt ggcagatcca
    38221 gatttcacta cggtcaaatc gccaaatcca gaagaacatt cagcttttga atatgctata
    38281 cgacttggag aaaaagaggg agctgacttg ctgattgcaa cagatcctga tgcagaccgt
    38341 ttaggcgcag cggttcgttt gccagatggt tcttatgaag tattgactgg gaaccaaatc
    38401 ggagcattgt ttgctcaata tattttggaa gcacatcaat caagaggcac tttaccagaa
    38461 aacgcagcgg tattgaaatc aatcgtttcc agcgaattgc ctacagcaat tgcggaacac
    38521 tacggtgcta ctatgttcaa tgtgttgaca gggtttaaat tcattgctga aaaaatccaa
    38581 caatttgaag aagaccattc acatactttt atgtttggat tcgaagaaag ttatggttat
    38641 ttagttaaac cttttgttcg cgataaagac gcgatccaag cattgctttt attagcagaa
    38701 gtagcagctt actataaaaa acaaggcaaa acgttgtatg acggcttgca aacgattttt
    38761 gaaacctatg gctatttcgc agaaaaaacg atttctgtga caatgagcgg acaagaaggt
    38821 tctgcgaaaa tctctgcttt gatggaaaaa ttccgtaaag aagcaccgac agaatttgcc
    38881 ggagcaaaag ttattcaaac agaagacttt aaagaattga cgagaacaga ccgtgaagga
    38941 aacgtagaaa aactaacaac accgccatcc gacgtattga agtatacatt ggaagaaaat
    39001 ggctggatcg ctatccgccc ttcaggaact gagccgaaaa tcaagtttta tattggtgta
    39061 aaaggaagct ctacagaaga cgcagccgaa aaagtagcct cttatgaagc gattatcaaa
    39121 aagatgacag aagaataaaa aacttgagac tgatggaaaa agtggtaaac tttagaagtc
    39181 agtctttttt caagttggag gtgaatggat tgccagacac gcaaaaatca aaagtagaga
    39241 aagtcagtca attattcaaa atgttgagtg accccactcg tcttaaaatt ctgctatatc
    39301 tgaaagacgg ggaacaaaat gtgacagcaa tcacccaagc agtggaaatg gagcagtctg
    39361 ctgtatcgca tcaactacgt ttattaagag aaaatcatgt cgtcaagtct catcgcgagg
    39421 gaaaagcgat tttgtatagt cttgatgatt ctcatgtact ggatattttg aaccagacac
    39481 tcaaacatgt ggaacatcaa taacgatagg tgcaaata
//
LOCUS       DAZBTF010000019        36510 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000019 DAZBTF010000000
VERSION     DAZBTF010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.019"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1093..1407
                     /locus_tag="ACHGNS_001061"
     CDS             1093..1407
                     /locus_tag="ACHGNS_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937023.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
     gene            complement(1460..2233)
                     /locus_tag="ACHGNS_001062"
     CDS             complement(1460..2233)
                     /locus_tag="ACHGNS_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937024.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
     gene            2338..2583
                     /locus_tag="ACHGNS_001063"
     CDS             2338..2583
                     /locus_tag="ACHGNS_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN6937025.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            2739..3590
                     /locus_tag="ACHGNS_001064"
     CDS             2739..3590
                     /locus_tag="ACHGNS_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HFN6937026.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSNTLDILYK
                     NTIFTNL"
     gene            3623..4300
                     /locus_tag="ACHGNS_001065"
     CDS             3623..4300
                     /locus_tag="ACHGNS_001065"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFN6937027.1"
                     /translation="MDKKVLIKRFSEAIEKGNAAIFAGAGLSMSQGYVSWPELLNDPA
                     TEIGLDSKKETDLVTLAQYYKNENGGSRGILNQILMDNFGEELEISENHRILASLPIE
                     TYWTTNYDHLIEKSIREAYKNPQVKKNYTQLATTNPNVDTIVYKMHGDIDDVSSTVIT
                     RDDYEKYDDSYALFKETLKGDLLTKTFLFLGFSFTDPNLERILSDIRWVLRENQRPHY
                     CIMRKIL"
     gene            4788..5753
                     /locus_tag="ACHGNS_001066"
     CDS             4788..5753
                     /locus_tag="ACHGNS_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6017 domain-containing protein"
                     /protein_id="HFN6937028.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            5775..5987
                     /locus_tag="ACHGNS_001067"
     CDS             5775..5987
                     /locus_tag="ACHGNS_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFN6937029.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            6062..6538
                     /locus_tag="ACHGNS_001068"
     CDS             6062..6538
                     /locus_tag="ACHGNS_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HFN6937030.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            6535..8343
                     /locus_tag="ACHGNS_001069"
     CDS             6535..8343
                     /locus_tag="ACHGNS_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFN6937031.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            8420..8635
                     /locus_tag="ACHGNS_001070"
     CDS             8420..8635
                     /locus_tag="ACHGNS_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family mobile element protein"
                     /protein_id="HFN6937032.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            8727..9590
                     /locus_tag="ACHGNS_001071"
     CDS             8727..9590
                     /locus_tag="ACHGNS_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB6/TrbL-like conjugal transfer protein,
                     CD1112 family"
                     /protein_id="HFN6937033.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            9627..10163
                     /locus_tag="ACHGNS_001072"
     CDS             9627..10163
                     /locus_tag="ACHGNS_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFN6937034.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            10177..10599
                     /locus_tag="ACHGNS_001073"
     CDS             10177..10599
                     /locus_tag="ACHGNS_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HFN6937035.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            10529..12928
                     /locus_tag="ACHGNS_001074"
     CDS             10529..12928
                     /locus_tag="ACHGNS_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HFN6937036.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            12960..14951
                     /locus_tag="ACHGNS_001075"
     CDS             12960..14951
                     /locus_tag="ACHGNS_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFN6937037.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            14974..15225
                     /locus_tag="ACHGNS_001076"
     CDS             14974..15225
                     /locus_tag="ACHGNS_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HFN6937038.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            15215..16444
                     /locus_tag="ACHGNS_001077"
     CDS             15215..16444
                     /locus_tag="ACHGNS_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1107 family mobile element protein"
                     /protein_id="HFN6937039.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            16441..18522
                     /locus_tag="ACHGNS_001078"
     CDS             16441..18522
                     /locus_tag="ACHGNS_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFN6937040.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            18671..22591
                     /locus_tag="ACHGNS_001079"
     CDS             18671..22591
                     /locus_tag="ACHGNS_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodL domain-containing protein"
                     /protein_id="HFN6937041.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKTD
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLIERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            22592..23536
                     /locus_tag="ACHGNS_001080"
     CDS             22592..23536
                     /locus_tag="ACHGNS_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937042.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            23540..23743
                     /locus_tag="ACHGNS_001081"
     CDS             23540..23743
                     /locus_tag="ACHGNS_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HFN6937043.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(24043..24279)
                     /locus_tag="ACHGNS_001082"
     CDS             complement(24043..24279)
                     /locus_tag="ACHGNS_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6937044.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(24462..24833)
                     /locus_tag="ACHGNS_001083"
     CDS             complement(24462..24833)
                     /locus_tag="ACHGNS_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HFN6937045.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(24830..26158)
                     /locus_tag="ACHGNS_001084"
     CDS             complement(24830..26158)
                     /locus_tag="ACHGNS_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFN6937046.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(26119..26448)
                     /gene="mobC"
                     /locus_tag="ACHGNS_001085"
     CDS             complement(26119..26448)
                     /gene="mobC"
                     /locus_tag="ACHGNS_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFN6937047.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(26706..27076)
                     /locus_tag="ACHGNS_001086"
                     /pseudo
     CDS             complement(26706..27076)
                     /locus_tag="ACHGNS_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            27221..27406
                     /locus_tag="ACHGNS_001087"
     CDS             27221..27406
                     /locus_tag="ACHGNS_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFN6937048.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            27564..27731
                     /locus_tag="ACHGNS_001088"
     CDS             27564..27731
                     /locus_tag="ACHGNS_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937049.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            28349..29008
                     /gene="vanR-B"
                     /locus_tag="ACHGNS_001089"
     CDS             28349..29008
                     /gene="vanR-B"
                     /locus_tag="ACHGNS_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFN6937050.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            29011..30372
                     /gene="vanS-B"
                     /locus_tag="ACHGNS_001090"
     CDS             29011..30372
                     /gene="vanS-B"
                     /locus_tag="ACHGNS_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFN6937051.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
     gene            complement(30463..31764)
                     /locus_tag="ACHGNS_001091"
     CDS             complement(30463..31764)
                     /locus_tag="ACHGNS_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFN6937052.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            32043..32849
                     /locus_tag="ACHGNS_001092"
     CDS             32043..32849
                     /locus_tag="ACHGNS_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HFN6937053.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            32867..33574
                     /locus_tag="ACHGNS_001093"
     CDS             32867..33574
                     /locus_tag="ACHGNS_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HFN6937054.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            33571..34542
                     /locus_tag="ACHGNS_001094"
     CDS             33571..34542
                     /locus_tag="ACHGNS_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFN6937055.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            34535..35563
                     /gene="vanB"
                     /locus_tag="ACHGNS_001095"
     CDS             34535..35563
                     /gene="vanB"
                     /locus_tag="ACHGNS_001095"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFN6937056.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            35569..36177
                     /locus_tag="ACHGNS_001096"
     CDS             35569..36177
                     /locus_tag="ACHGNS_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFN6937057.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(36243..36416)
                     /locus_tag="ACHGNS_001097"
     CDS             complement(36243..36416)
                     /locus_tag="ACHGNS_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937058.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         9800 a         8765 c         9416 g         8529 t
ORIGIN      
        1 tacgcgtacg attattaaaa ataataaatt ttcactagaa gaaaatggaa ataaaatcaa
       61 ataattagga agaaaaaaca aagcaattag aaaagcctgc aagcaaattt gagtaatcga
      121 gatccaattt ttttaaggaa gtttaacaaa aatatgtaat tctattatca ctattaaaga
      181 gaaagttgga gcggaatata aagaaaagag aaaagtaatt aaacgcaaaa aaatgtagaa
      241 cttaaaaaat ataaagagga acaaccgtta aaattaggaa tgatgttgtg taaacattat
      301 aattgctatg atataaatga agtgttaaag aaagcaaatt taaaccaatt ttgtgaacag
      361 aaaagcctta gtcactatga ccaaaactac ggacgaaact atgaccaaaa gtaggtacta
      421 aattatgacc gaaaataggt ccaaaatgga tttttttatg atatcaaaaa ttatgaaatg
      481 attttgaaag aaaaccagtt ggcacagcca acttagccac cttcctgtag gaaggtgcta
      541 tcagttttcc cttatgctct tttctttctt ttttgtttca tatgtcaaat gccattttga
      601 aatgtaccat cttaagaaat ttatccttaa aaacaattgc gttattggat aaaaagatgc
      661 tacattatct taaaaaagga atcaaatatg atgaatttag tggctagcat atcgtgtgat
      721 taagatactt tatagtgtaa gttgaaggag aaaaaatatt taaaatatac ttcccatttc
      781 agtgggaagt aaaattaatc ttgttgtttc tactagtaat gtatcttttt ggaaactatg
      841 taacgatgta aacaagattt ttttcagaag aaaaaacgat aaaagttgtg ataaacgatt
      901 taaaaaatag gagaaaatat atcaagcgca tggtatgaaa cgcacattta aaaagattcg
      961 actaactaaa aaaataagtt tacaaagcat tgttgtgtat tacaagtgat ctatacactg
     1021 aaaaatgata gagaaaatct gatacgtccg caacaatgga caattgaaaa tgaaagtgag
     1081 gaaattggaa gaatggctga tgaaaataaa gttgttccgc ttgaaacact agtgataaat
     1141 aaggaagagt ttgagcaact ttttcttgat ttttttgttc aatatgacag gatttatgat
     1201 tttagttcgt cactagattt atttgaatta gaggcagaaa taatagaaaa cagtcgagag
     1261 gagaatggta aaatcatcat ttcagtagaa aaattcaatg aattattctc agaaacattt
     1321 gaaatagata cagattatta tcaaccacaa gaagttttcc aatcatttag aaatcagatt
     1381 attcgctcag gtaagaccgt aaactgaaaa acaataaaag aaacgttgaa agaataatta
     1441 ttccttcaac gtttcttttt tagaatgttt taatgaccaa agcagtttca ttgtcaggct
     1501 taggtaattc atgaaatccc gtatttatag tgctcaattg atcaactaac catgttttac
     1561 tttttgaatt gtagataaat ctaaaattaa tatcgttatt atcgtgttga aggggcgtct
     1621 ccaaataatc tgctgtatat tcatagtcaa cgtaacctgc taatacaaaa gagagtatcc
     1681 ctttatttaa tgttagattt aatggataat catacgactt tatttttttc aacttcatat
     1741 tgtacatatc ttcagccata aaagttgagt tcgtgatttc atcttgggtc tcattaaaaa
     1801 agttatccga ataatcgctt atttttgaaa agtctttatt ggcaccagcc tcaaaaaatt
     1861 gttcaacttt ttcagtataa agttgtgcta acgctccttt ttcaatatta actttaatat
     1921 tttcatgtct atcaatttgt gtgctgttca gtaagggaag atcagctttt agttttatat
     1981 cgtcttgatt tctttcaatt tcaattggca aaatcttttc agtagattca gtttctgata
     2041 aaggaacatc tagtttttta tcatgataat aagcttcaaa tccatctgga accggaaaaa
     2101 aatgtattgg gtaattcttt tctgaggttg ttgtactagt ttccgtactt gtagaacttg
     2161 tgtcagaagt ggtacacccg cttatagttc cgatgagtaa agcgcaagtt agaatgattg
     2221 caaatttctt caaaaaaatt cctccttaaa attgaatatg gactaatact agtcccattg
     2281 tagcataccg aatctttaag attaataaga aaaaggacta attttagtcc aaaaggtgtg
     2341 ctattcttga aagaggaaaa aattattgat aaagaaaatg taatcggtag caatattaga
     2401 cgagtccgat tagaaaaagg aattggtcaa accgagctga ttagagacct acagcttcgt
     2461 aaaattgcta taactaggga aacgttggtt aagattgaag gtggccgcca acacattaag
     2521 ttggatcaat tgaaagccat caaagatatt ctacaggtgt cttatgaaga tttgcttcaa
     2581 tgactacttg tagtcatttc gtttgaaatt attatgttac acccttatgt gtcacgaaga
     2641 aatactggta taataatgaa tgggatatgt aacgataggg tatactgtta tgttgcctaa
     2701 taaattgtgg atcaaaatac aagaatggag ggatatcctt gtataaggca tatgaattgg
     2761 attttacaaa tgctgactta aatgcattct cctcaagctc gcaatcatat acacatgtag
     2821 taaatcaaat agatcacaac caaaaatcta ttaacaagag aatagaaaat ctatttgaaa
     2881 gtgaaaatga tttagttgat tattattcta aggacagcaa aattctagat gcagacgaaa
     2941 ttattaaaga ttggtttcca actataaaag cagatatttt tatatctcac tctcatgctg
     3001 atgaaaaatt agctatacgt tttgcatcat ggctatttga aaattttggt cttactgcat
     3061 ttattgattc ttcggtttgg ggatacagtt ctgatttatt aaaaaaaata gaccaaaaat
     3121 attgctataa ggaacaaaca aaaacatatg attatgataa aagaaatgta acaactagtc
     3181 atgttcatat gatgctttct acagctttaa ataatatgat tgatagtaca gaatgtctgt
     3241 tttttcttaa tacacctaat tcaattagtt tgagcaatga aatcactaat gaacagaaat
     3301 ttacgtattc gccatggcta tatagcgaac tgacgacagc ttcaatagtg gaaaaaaaga
     3361 atccacgatt ggaaagtaat cctcaaatga gcaccgagga tgttagaagc attataaagc
     3421 actattcttc gcaagaaaat ctgcaaataa aatattcttt aaatttaagt gaattaactt
     3481 atttacgaaa agctgatttg aaaaaatggg ctttagatgc tgtggaatgc actaaaggaa
     3541 gtaatacatt agatatttta tataaaaaca ccattttcac taatctctaa tcaatttact
     3601 gaatcgaaag gaaccgggta gaatggataa gaaagtttta ataaagaggt tctctgaagc
     3661 tattgaaaaa ggaaatgcag caatatttgc aggtgcagga ttatcgatgt cacaaggata
     3721 tgtaagttgg ccggaactac ttaatgatcc tgcaacagaa atcgggttgg attctaaaaa
     3781 agaaacagac ttagttactc tggctcaata ttataaaaat gaaaatggtg gatcaagagg
     3841 aattttgaat cagatactta tggataattt tggtgaagaa ttagaaatat ctgaaaatca
     3901 tcgaatacta gcaagtttac ctattgaaac ttattggaca actaattatg atcatctaat
     3961 agaaaaatca atccgagaag cttataaaaa ccctcaggtt aagaaaaatt atactcagtt
     4021 agcaacaact aatccaaatg tggatacaat tgtttataaa atgcatggtg atatagatga
     4081 tgtcagttcg acagtaataa caagagatga ttatgaaaaa tatgatgatt cgtacgcgtt
     4141 atttaaagaa actttaaaag gagatttgtt aacaaaaact tttctctttt taggatttag
     4201 ttttacagac cctaatttag aaagaatttt atcagatata agatgggttt tgagagaaaa
     4261 ccaaagacct cattattgta taatgagaaa aatattatag aaaaaatttt aggttatcgc
     4321 tgaaagcccc ggaaatacgg gcatttcgca ggatatgaga actcaattta ctaccaattt
     4381 actactttcg gattgagccg catatatgct gaaccatact gctaataccc gccaccccaa
     4441 atacggcaca tcataaattg aatacgacac cgtaaacgcg gcgttcctct ttcccgtcat
     4501 gggagaacag gacgccgctt ttttatgccc aaatagagaa aggaggcgac gccatgctat
     4561 ttacttccgg ccctgaccgg gcgtttgaac ggctgatgca ggaaaagccc ggtttcgatc
     4621 actacgacgg cggcggggcg gacgcgccgg aggattgcgg cacctgccga ttttaccgcc
     4681 cggattggaa atatcaattc tgccagtttg cagagtgtcc ctatcagccg ggcaagctga
     4741 ccgactatgc agcggtcaaa ttcacagtga aaggagcgaa caacgctatg gcagttttcc
     4801 gtgtggaaaa gaacaaaggc tatacggtga tgtcaaatca ccacctgcgg aataagaacc
     4861 tgaccttaaa ggccaagggc ctgctgtcgc agatgttgtc cttgccggag gattgggact
     4921 ataccctaaa aggactgtcc tatatcaacc gggaaagcat cgacgcgatc aggacagcgg
     4981 tatgggagct tgaaaaagct ggatatatcc ggcgtgagca gggccgtgac gcaaaaggca
     5041 aaatggccga tatggtctat accatctacg aacagccggt attggaaaac ccggtattgg
     5101 aaaatccaac atcggataac ccggtgttgg aaaatccgac atcggataat ccggcgtcgg
     5161 aaaatccaac gcaattaaat aaagaagtac aaagaactga cttatcaaaa aaagaaaaaa
     5221 taattacaga tggacaaagt acccattcca ttcctatccc atccccgacc cctgccccta
     5281 tggagggcga ggcagcggct acgccgccgg aacggaaaag gacaggaaag gatgaggctg
     5341 tacggattta tcgggaaatc attttggaga acatcgagta tgcctatctc attcaggaca
     5401 ggagcattga ccgtgaacag cttgacgaaa tcgtagacct gatgctggaa accgtctgca
     5461 ccgcccgaaa gacaatccgt gtcgccgggg acgactaccc tgccgagctg gtgaaatcca
     5521 agtacatgaa gctgaccggc gaacatatcc gctttgtcct tgactgtctg cgggagaaca
     5581 caacgaaagt ccgcaatatc aaacaatacc tgcgggctat gctgttcaac gcccccagca
     5641 ccatttccaa ctactacacg gcccttgtcg cgcacgacat ggcacagccc gattggggaa
     5701 agcctaaatc cggcctgccg gactattcct gcggcgaggg cgaaagcctg taacgagaaa
     5761 aggaggacga ccctatgaag cagggcgcat tgatttttga cgagcgaaca gaccgttacg
     5821 acatccgctt tgatctggcc gactattacg gcggcctgca ttgcggggag tgtttcgacg
     5881 tgatgatcgg cggcaaatgg aggcccaccc gcattgaaat ggccgaaaac tggtatcttg
     5941 tcggcatccg ggccgacgac ctttccggcc tgcgggtgcg gatttgacct atatctacct
     6001 gcggcgtctg gtggttccgc cttagacgcc gcttttgact ttctgacaag gagggatgcg
     6061 attgcaggaa gaagttactc aaaagaccat tgccctgtcc atgaaaacgg gcaagctcac
     6121 ggctcaagtg ctgcaagcgg cgctgaaaaa gtacctgcaa caccgggcca agggcaaaaa
     6181 cacgctgcac cacggccagc agagcctaaa gcagcttaaa aagcatgggg ctgatctctc
     6241 caacattgag atcaccgagg ccaacattgg agcgttcaag ccatgcgcca agaaatacgg
     6301 cgtggatttt accttacgaa aagataagac cacccagccg ccccactacg ttgtgatttt
     6361 caaggccaag gatgcagata accttgatct ggcgtttcgg gagtttaccg ccaagacgct
     6421 ttccaaggag cagcggccct ctatccgcaa ggttttgtcc gctgtgaagc aaaaggcggc
     6481 ggcccagacc aagcagcggg ccaaggagaa aatcaaggaa agggggctgg aactgtgaag
     6541 cctgacgtaa agaaattgct gatcctgaac ctcccgtatc tgatttttgt gtacctgttt
     6601 gcgaaatgcg gggaggcgta ccggctggcc gctggtgctg acctgtcggg gaagctcctg
     6661 cattttgcgg atggttttgc ggcggctttt gccaaccctc tgccaagcct gcacctgttt
     6721 gacctgtgta tcggtatcgc cggtgccgtg atcgtgcggc tggtggtgta ctgcaagagc
     6781 aagaacgtga agaaataccg caaaggcatg gagtatggca gcgcccgctg ggggacggcc
     6841 aaagacatag cgccttacat cgaccccaag tttgaaaata atatcctgct cacccaaacg
     6901 gaacggctta ccatgaccgg caggcccaaa gaccccaaga cagcgcggaa taaaaacgtg
     6961 ctggtgatcg gtggcagcgg ttcagggaaa acgagatttt tcgtgaaacc caacctgatg
     7021 caatgctttc cgaccacgga ctatccgact tcgtttgtgg tgacagatcc gaaaggcact
     7081 ttggttctgg aaaccggcag gatgttccag caggcgggct accgcattaa aatcctgaat
     7141 accattaact tttccaagtc catgaagtac aacccctttg tctacatcca ttcggaaaag
     7201 gacgtgctaa agctggtaaa taccctgatt tccaacacca agggcgaggg ggaaaagtca
     7261 gcggaggatt tttgggtgaa gtcggaacgg ttgttttatt cggccctgat cggctatatc
     7321 tggtacgagg ccccagcgga ggaaatgaac ttctcaacgc tgctggaaat gataaacgcc
     7381 agtgaagccc gcgaggacga cccggagttt caaagccccg tcgatctcat gtttgagcgt
     7441 ttggagcaga aagaccccga ccattttgcg gtgcgccagt acaagaaatt cctgctgtcc
     7501 gcaggcaaga cccgttcttc catcctgatt tcatgcggtg ccagacttgc gccctttgac
     7561 atccgggagg tacgcgagct gatggaggac gacgaaatgg agctggacac catcggggac
     7621 gaaaagacgg tgctgtatct cattatgagc gatacggaca cgacctttaa tttcattctg
     7681 gctatgctcc aaagccagct aatcaacctg ctttgtgacc gggctgatga tgtgtacggc
     7741 gggcggctgc ctgtccatgt acgcctgatt ttggatgaat ttgccaacat tggccaaata
     7801 cccaatttcg ataaactcat tgccaccatt cgaagccgtg aaatatcggc ctctattatt
     7861 ttgcagagcc agtcgcagct aaaagcgatt tacaaggacg ccgccgagat catttcggat
     7921 aactgcgata gtgtgctttt ccttagtggg cgcggcaaaa atgccaagga gatttccgat
     7981 gcgctgggga aagaaacgat tgacagtttc aacaccagcg agaaccgggg aagccaaacg
     8041 tcccacggat tgaactatca aaaattagga aaggagttga tgtcccaaga cgaaattgcc
     8101 gtgatggacg gcggcaagtg tatcttgcag ctacggggtg tgcgcccgtt tttatcggag
     8161 aaattcgata ttaccaagca tccccggtac aaataccttg ccgacgccga caagaagaat
     8221 acctttgacg tggacagatt tttgactatc acgcgccgga aacggcagca agtggtaacg
     8281 caggatgaag tattcgacct gtatgaaatc gacctgtcgg acgaggacgc cgccgctgaa
     8341 taaacagcgg cttttttcat tccaacaaca aaatcaaaaa atcattatga gccggaaacc
     8401 ggcagaaaga ggtaaattta tggcattttt taattccgca gttactactt tgcagactat
     8461 cgtgatcgga ctgggcggcg ctctgtgcgt atggggcggc atcaatctgt tggagggata
     8521 cgggcaggac aatccggggg caaaatcgca gggtgtcaag cagcttgtcg ctggcggcgg
     8581 cgtggccctg atcggtgtca ctctggttcc cctgctgtcc gggctgctgg gctgatcctg
     8641 gcctttgtcc ctgtccaaac ccttaactaa tcgcctccgc gtcctcccgg ccagaccggg
     8701 agggcgcggg aaaggaggtg ctacccgtga ttagtgaaat cattgaagaa tggataaaag
     8761 gcatcctcat ggacggtatc acgggcaacc tgtccgggct gttcgatacg gtaaatgcca
     8821 aagtcggaga aatcgccgcc gacgtgggac gaaccccgca ggcatggaac agcggtattt
     8881 tcaatatgct gcacagcctg tcggaaaccg tgatcgtccc catagccggg gccatactcg
     8941 cccttgtcat gtgctatgag cttatccaga tgataacgga aaagaacaat atgcacgact
     9001 ttgacagctc catgttcttc cgctggatat tcaaatccgc gtttgcaatc ctgatcgtaa
     9061 cgaacacatg gaacatcgtc atgggcgtat tcgacgccac ccaaagcgtc gtcaatcaaa
     9121 gcgccggtgt gattatcggg cagacatcca ttgattttaa caccctgata ccagaccttg
     9181 aaagccaatt ggaggctatg agcgtgggcg ctcttttggg gctgtggttc caaacccttg
     9241 ttgtgggcct caccatgaat atcctttcca tctgcatttt ccttgtgacc tatggccgca
     9301 tggtggaaat ctatgttgtc acgtcgctgg ggcctatccc ccttgccaca atgggcaacg
     9361 cggaatggcg cggcatgggc caagggtatc tgaaatccct gctggcgctg ggcttccaag
     9421 cctttttaat catggttgtg gtgggtatct acgcggtgct gatacagaat atcggttccg
     9481 cttccgacat atccggcgca atctggggct gtatgggcta tacggtgctg ctgtgcttct
     9541 gcctgttcaa gaccggcagt atatcaaagg ccgtattcgg ggcgcattga tcccaaaatc
     9601 caagcataca caggaggaac ttgtttattt caacgtacaa catcatttac gctgacccgc
     9661 cgtggcggta tgactgcaaa aacgtacagg gcgcagcgga aaatcactat tccaccatga
     9721 gcattgacga gctgtgcgcc ctgccggtgg aacggctggc gtccaaagac tgcctgctgt
     9781 ttttgtgggc cacattcccc atgctgccgg aagctctgcg gttaatcaaa gcgtggggct
     9841 tttcattcaa gaccgtggct tttgtatggc tgaaacagaa ccgtaaaagc cttacatggt
     9901 tttacgggct gggccgctgg acaaggggaa acgcggaaat ctgcctgctg gcaacacacg
     9961 gcaagccgaa gcgtcgttct gcctctgtcc atcagtttat tatcagcccg attgaacaac
    10021 acagcaaaaa gccggatgtg acccgtgaaa aaatcgtaga gctggcgggc gatttgcccc
    10081 gcgtggagct gtttgccaga cagaaaaccc ccggctggga cgtgtggggg aacgaggtgg
    10141 aatgtgacct tatcctgacg tgaaaggagg tgccaaatgg cttatgtaac cgtccccaag
    10201 gacttaaccc atgtgaaaag caaggttgtg tttgggctta ccaaacggca actgatctgt
    10261 ttttccgggg cgctggtgat cggcgtcccc ttatattttc tcacccgtga ctatcttacc
    10321 aacagcgcgg cggccctgct catggtattt gccatgctgc cgggcttttt gcttgccatg
    10381 tacgagcgtc acggccagcc ccttgaagtg gtgattatgc agatcatcca gtgctgcttc
    10441 atccagccaa aggagcgtcc ctatcagacc gacaacacct acaccgccct tgtgcggcaa
    10501 taccagatgg aacaggaggt aaaggccatt gtccaaaaag gaaatcccaa gagaaaccga
    10561 aaaaacaaag ctgacccgcg cccagcgaaa agaaattgac gcggtaatcc gtaaatataa
    10621 gggcgacggc aggccccaca cggcgcagca gagcatcccc tacgaggtca tgtacccgga
    10681 cggcgtgtgc cgggtatcgc ccggcgtctt ttccaagtgc attgagtttg cggacatcag
    10741 ctatcagctt gcccagccgg acacgcagac ggctatcttt gaaaaactgt gcgacctgta
    10801 taactatgtg gacgcctcca ttcatatcca gttttctttc ctcaatcgga aagttgaccc
    10861 ggtgcagtac gccaagagct ttgagatcgc gccgcagggg gacgacttcg acgacatccg
    10921 ggccgagtac accggcattc tgcaaaagca gcttgcaaac ggcaacaacg gcatggtaaa
    10981 gaccaaatac ctgacattca ccatcgaggc cgaaagcgtg aaagcggcgc gagccagatt
    11041 gaagcgcatc ggctttgacc tgttaggcta tttcaagagc atgggggccg tagcccatgt
    11101 gatggacggc tgggaacggc tgaacctgct gcatggcgtg taccacccgg acggcgagat
    11161 attcaatttt gactggaagt ggctggcccc gtccggcctc tccaccaagg attttattgc
    11221 cccgtcctcc ctgtgcttcg gcaatgcaaa gacattcggc atgggcggca aatacggcgc
    11281 ggtaagtttc ctgcaaatcc tgtccccgga gctgtcggac gatatgctgg ccgacttcct
    11341 caatacggaa agcggtgttt tagtcaatct ccatgtgcag gccattgaac agaccaaggc
    11401 gatcaagacc atcaagcgca aaattaccga ccttgacgct atgaagattg cggagcagaa
    11461 aaaggccgtc cgcagcggtt acgatatgga cattcttccc agcgaccttg ccacctacgg
    11521 cgaggacgcc aagaagctgc tgacaaagtt gcagacccgc aatgaacggc tgttccagtt
    11581 gacgttcctt gtcctcaacg tggccgacac caagcaaaag ctgaataacg acgtgttcca
    11641 agccgcaggc gtggcccaaa agcataactg tccccttgtc cggctggact accagcagga
    11701 acagggcctt gcaagcagcc tgccgctggg cgtcaatcag atcaagatac agcgcagcct
    11761 tacgacttcc agcgtggccg tgttcgtccc ctttgtgacg caggagcttt ttcagggcgg
    11821 cgcggccatg tattacggca tcaacgccaa gtcccgcaac atgattatgc tggacaggaa
    11881 gcaggcccgt tgccccaatg ccttgaagct cggcaccccc ggcagcggca aatccatgag
    11941 ctgtaaatcc gagatcgtca gtgtgttctt aacgacgcct gacgatattt ttatttcaga
    12001 cccggaggcc gaatattacc cccttgtcaa acggctccac ggacaggtaa tccggctgtc
    12061 gcctaccagc aaggactttg tgaacccgct ggacatcaac ctgaattatt cggaggacga
    12121 taacccgctg gccctgaaat ccgactttgt gctgtcgttc tgtgagcttg tcatgggcgg
    12181 caagaacggg ctggaagcca ttgaaaagac cgtgatagac cgggctgtgc gcgtgatcta
    12241 ccggccctat ctggcagacc cccgcccgga gaatatgccg atcctgtccg acctccacaa
    12301 agcgttgtta gatcagcatg tcccggaggc tgaccgggtg gcgcaggccc ttgacctgta
    12361 tgtgtccggc tccctgaacg tatttaacca ccgtaccaac gtggacatcg gcaaccggct
    12421 tgtgtccttt gacattaagg agctgggcaa gcagctcaaa aagctgggga tgctcattgt
    12481 ccaagaccag atatgggggc gcgtcacggc caaccgaagc caaggaaagg ccacatggta
    12541 ttttgccgac gagttccatt tgcttttgaa agaggaacag acggcggcct attctgcgga
    12601 gatttggaag cggtttagga aatggggcgg cattccaaca ggtgccaccc agaacgtcaa
    12661 agacctgctt tcctccccgg aaattgaaaa tatccttgaa aacagcgact tcattacgct
    12721 gctcaatcag gcgtccgggg atagaaaaat ccttgcggaa cgcttgaatt tgtccaccga
    12781 acagcagaag tacatcgaca attccgaacc cggcgagggc cttttgatct ttgaaaacgt
    12841 ggtgctgccc tttaccaacc ccatcccgca caacacccag ctttacaaga ttatgacgac
    12901 ccgcttaaac gaggtggcgg gagtatgacc cggcgacgcc acaaaggagg tgaaccacca
    12961 tgaaagagct gaaagccaag gcgaaagtga cgcaggccat gacccgcgac gggctgacgc
    13021 tggaaaatca ggccaccggc gaggtggaga acatttccag ccgggaggcc gaacaggatt
    13081 tgtccgcgcc ggggccgggc ggcaccgccg ataagctgct ggaacgggcc gacgccatcc
    13141 atgacggcca taaggccaag aaagccgcga agcaggccgg ggctgtggcc gccgaggggg
    13201 acgctgccct gcaccggcca tccgcccggc tccaactgtc ggaggaagaa cgggccgacc
    13261 cggatttaca gccctatatc cgaaaatcgg acaaacgggc cgataagctg gataaggccc
    13321 gcgccgccat ccccaagaag cgcgtcccgg tcaaggaaac cgtctttgac gaggcaagcg
    13381 gcaaagcaaa gaccgtcctg cactttgaca aacaggacaa ggggcctccc tccctgaaac
    13441 ccaatccggc aggccgtcct gtttccgagg tttttctatt cggccacggg aaaatccatg
    13501 aagtcgagca tgaaaacgtg ggtgtggagg gcggccataa gggggaggaa cttgccgagc
    13561 ggcaggccgg aaaagccatc cgcagcacca tccggcacca caggctgaaa ccctactggg
    13621 acgcggaaaa ggccgagcgc aggctattgt ctgccaacgc ggagtatttc taccagaaat
    13681 ctttgcggga taacccggag atcgcacagg cggccagcaa ccccatttcc cggttctggc
    13741 agaaacagca gatcaagcgc cagtacgcca aggccgcccg gacagcgggc cagaccgcag
    13801 gcgcggcggc acagggcgcg gccaagacag ccggggcctc cacgaaagcg gccaaggccg
    13861 cagccaaaga aagtgaacgg gcggcctcct ttgtggcccg ccactggaaa ggggcgctgg
    13921 ccgtccttgt gttggggctt ttgatgctgc ttatcatcgg cggcttacag tcctgcaccg
    13981 ccatgttcgg cagcgccgga agcggcatca cggcgtcctc ttacttttcc gaggatagcg
    14041 atatgttggc cgccgaggac gcctacgcgg agctggaagc cgagctgcaa gactatctgg
    14101 acaactacga ggccacccat gactatgacg aataccattt tgaccttgac gagatcagcc
    14161 atgaccccta tgtgctgatt tccattctgt cggcgctcca tgacgggata tttaccatcg
    14221 gggaggtaca gggtgatttg tccatgctct ttgacaagca atatatcctg acagaaaacg
    14281 tcgtggtaga aacccgttac cgcaccgaaa ccgatacatg gacggacgcg gagggcaaca
    14341 cccatacaga cacctaccaa gtgccgtatg actattacat ctgctacgtc acgctggaaa
    14401 acttcgacct gtcccatgtg ccagtctata tcatggacga ggaaacgcta agtttctatt
    14461 ccctgtatat gtccacgcta ggaaaccggc ctgatctgtt ttccggcagc cagtacccca
    14521 acgcctccac cctgacgccg ccgacctact acgacgtacc cccggaggct ttggaggatg
    14581 ccacctttgc cgccatgctc aaagaggcgg aaaaatatat cggttatccc tatgtgtggg
    14641 gcggcagctc tcccagcacc agctttgatt gcagcggctt catttcgtgg gtaatcaatc
    14701 atagtggatg gaacgtggga cggctggggg cgcagggcct atataacatc tgcacccctg
    14761 tttccagttc gcaggccaag cccggcgacc ttgtattttt caagggaacc tacgacaccc
    14821 ccggcgtgtc ccacgtcggc ctgtatgtgg gcaacaatgt gatgctgcat tgcgggaacc
    14881 cgatttccta cacgaacctt acttcaaact actggcaatc ccatttttac agttacgggc
    14941 gtttacctta acgccagaaa ggagctttca attatggcaa ccaaacttga ccgcatcgaa
    15001 aaggacatcc agaaaaccaa gtctaagatc gcggagtttc agaaacagct tcgggagctg
    15061 gaaacgcaga aaaccgaaca ggagaatttg cagatcatcc agcttgtgcg cggcatgaac
    15121 atgaagccgg aggaatttgc cgcgttctta cgcagcggcg caatgcaggc cgcccctgcc
    15181 gcaacccctt accatgaaca ggaggacaat gcccatgaag aataagacca gacggatttc
    15241 ccgtattctc cccgcacttc tggccgtgat gctctgcgtc acggcctttc ccgttaccgc
    15301 ccttgcaggc ggcaatgacc cggcaccggc cccgctgccg gaggctacaa ccgaacccgc
    15361 tacgggcggt ttggagcctg aaacagacga aaccggcacc gccacggacg ggacagaaac
    15421 cgaggccgaa aaagataccg gccctctgac cggcaaggac atcaccgacc tgttttccgc
    15481 gctgttcggt tccaaggtga gcattgccgc cacggacgac ggcatccaga tcaccaccgg
    15541 caccgacaaa aaagaacctg aacagaccgg caccgtcacc accaacggcg gcaggctgaa
    15601 tgtccgcacc ggcgcggggc ttgacaagac cgcgttcacc cagcttccca atgggacgac
    15661 ggttgaggta gtcggcacag acggcgactg gattaaaatt ctcttgccgg agcgcatcgg
    15721 ctatgtccat tccgactata tgaccgtcag cgaaaaggaa gtagcggcta ccggcgaggg
    15781 cggtttttcc ctctccattg acccggagga aattgcttct ctgctggaac tgttcaatgg
    15841 cggcggggcc agcgccgccc tgtccccgga cgggaacctg tcactgattg acgacatcgg
    15901 cagcgccgcg aaaaatggca agcagtttat caccgtggaa accaaaaacg gcaatgtgtt
    15961 ttatctgatt attgaccggg acgacgaggg aaaagaaact gtccatttct taaatcaggt
    16021 ggatgaagcc gatctgctga cgctgatgga ggacggggaa accgtcaccc ccgctgctgt
    16081 ctgctcctgt accacgaaat gcaaggccgg ggctgtgaac acgaattgcc cggtatgtaa
    16141 aaccaacctg accgagtgca gcggcccgga gccgcaggaa ccccagccgg aggaacccga
    16201 agccccggag gaagaaccca agggcggcgc gggcggcctg atcgtgttcc tgctggtggc
    16261 gctggcgggc ggcggtgcgg ccctttacta tttcaagttc aaaaagccca aggctgaaac
    16321 caccggcagc gacgatttgg gcgagtatga ttttggggag gacgaggatt tggaggacga
    16381 ggaacccgaa accgagtttg acccggaagc tgattttgtg agtgaacagg aggacgagga
    16441 atgacatata aacttgtaat cgcggaaaaa cccagcgtgg cccgcagcat tgcgggcgtg
    16501 atcggggcca cggagaaaca cgacggctat ttggagggga acggctatct ggtgagctgg
    16561 tgcatcggac atctggtgtc ctttgccgac gccggacgct atgacgagcg tttcaaaaaa
    16621 tggcgctatg aggatttgcc cattctcccg gaaacatggc agtacatcgt ccccgacgac
    16681 aagaaacagc agtttgacgt tctctgttcg ctgatgcagc gccccgacgt gaccggcctt
    16741 gtgtgcgcca ccgacgccgg gcgcgagggg gaattgattt tccgtttcgt ctaccagatg
    16801 gcgggatgcc aaaagccctt tgagcgcctt tggatttcct ctatggagga cagtgctatt
    16861 aaggacggct ttgcccacct gaaaccgggc ggcgactatg atccgctgta tcaatcggcc
    16921 ctgtgccggg ccaaggccga ctggctgatc ggcattaacg ccacccggct tttttccgtc
    16981 ctgtaccaca agaacctgac tgtgggccgc gtccagacgc cgaccttaaa aatgctggtg
    17041 gacagggacg ccaagattac cgatttcaag aaagagaaat atcacatcgt ccatatcacg
    17101 gcgggcggcg cggacgcggt aagcagccgc ttttccgacg ctgccgaggc aaacaccgtc
    17161 aaggcggctt gtgcaaaagc gcaggccgtt tgcgtttccg tgacgcggga gaaaaagacg
    17221 gaacagccgc ccaagctcta tgaccttacc accttgcagc gggaggctaa ccggcttttc
    17281 ggctataccg ccaagcagac ccttgactat gcccaggcac tgtatctaaa gaagctgctg
    17341 acctatccga gaacggatag tcaatatctt accgacgata tgctgcccgc cgtggaaagc
    17401 cttgtgtccg gcctgtggga gctggtgccg tttgccaagg gcctgaacct ttccccgcag
    17461 tttgaccgca tcctgaacag caaaaaggtg tccgaccacc acgccattat ccccaccgct
    17521 gaatttgtga agcagggctt tggcactctg gccgagagtg aaaaaaagct gctgtccctg
    17581 atctgctgca agctcttatg tgccgtggcc gggccgcata tctatgaggc cgtcaccgcc
    17641 acatttacct gtgccgggca ggagtttacc gccaagggca agaccgtcct ttcccccggc
    17701 tggaaagaga ttgaacgccg gttccgttct tccttaaaga ccgacgccga cgaggacgcc
    17761 gaggcggtgc gggagctgcc ggagctttgc gagggccaga cctttgcaga cgtggccgcc
    17821 tctgtcaccg agcatttcac cacgccgccc aagccctaca ccgaagatac gcttttgtcc
    17881 gctatggaac gggccggggc cgaggatatg ccggaggatg ccgagcgcaa gggattaggc
    17941 acaccggcca cccgcgccgc cattctggaa aagctggtgc agatgggctt tgtccagaga
    18001 aaaggcaagc agctcattcc caccaaggac ggcatcaatc tggctgtggt gctgccggag
    18061 gcgcttactt ctccccagct tactgccgaa tgggaaaacc ggctgaccga gatcgccaag
    18121 gggcaggccg atcctgacga atttatggac ggcatcgaga cgcaggcgcg ggagctggtg
    18181 cagacctatt cctgtatcag tgaggaaaag cagaaattgt ttcaggccga gcgtgttcct
    18241 atcgggacgt gtccccgctg tggtgaggct gtctatgagg gcaagaaaaa ctactactgc
    18301 ggaaaccggg cctgccagtt cgtcatgtgg aagaatgacc gattttttga ggaacgcaag
    18361 aaagcgttca ccccgaagat cgccgctgcg ctgctcaaaa ccggcaaagt caaggtaaag
    18421 ggcctctatt ccgtaaagac cggcaaaacc tacgacggga cggtgctttt ggctgatacc
    18481 gggggcaagt acgtcaacta ccgcattgaa cacaggagct aaaggacagt aacacagcaa
    18541 gcatagggag tgtggctaca ctcccggtaa aatcccccgg ccgcgtccct gccgggccgg
    18601 ggatttttgc ttgttgggga agtccccaac cccctatttt ttgaaacaga acagagaaag
    18661 gagtgaccgc gtgaacgaag tattttctat ccaagtccat aaccggcagt ttttggaaca
    18721 gggcaaacaa ggcgtctggc tggatttgcc caccacaacc gaaacgctgc aagccgccct
    18781 gcgggatgtt ggcattactg ccgacaatcc gcaggatttt tttatcaacg ggtattcctg
    18841 cccggaggac agacaccttg ccattcccta tgacatggtg ctggccgccg acgtggacga
    18901 gctgaatttc cttgccgccc ggctgggcca gcttgacgcc gccgagatcg cagagctgaa
    18961 cgccgccctg caaaatcccc gtggcggctt tgagaatatc ggccagatca tcgactacac
    19021 cgaaaatgtg gattactatg tgcatcttcc cgacgttagg ggggatggac agttgggcga
    19081 ctactacctg aaccgttccg gcatggtgga tatgccggag gaatggaaag ccgggattga
    19141 tacggcgcag tttggacgcc acattgccca gcaggaacaa ggcgttttta cccagtacgg
    19201 ctatctggtg agaagcggcg acgaatggca gcgcgtccat gagggccagc cggtgccgga
    19261 ggaataccgg gtgatgcact acccccagcc ggaagctgac cgggaggaac cgcaggccca
    19321 gcagccggag gcatccgaaa gccccgacaa agcgccgccc ccggttatcc cgatcatgct
    19381 ggacggcatg aacagcaaag aacgcatgaa agtgattact gaccgtctgg aaaccggcgt
    19441 acaggagctt tttgaaagta gccgctatat ggcttactta tccgctatgt ccaagatgca
    19501 cgattacagt ctgaacaata cgctgctgat cgccatgcag ggcggttcga ttgtggcggg
    19561 ctataacagg tggcaggatg aatttgaccg ccatgtgaag cggggcgaga aaggcattaa
    19621 gatactggcc ccggccccgt tcaaggttaa gcagcagatg gagaaaaccg acccggccac
    19681 ccagcagccc gtgatcggcg cggacggcaa gcccgtgacc gaagaacagg aagtgacggt
    19741 tccggccttt aaggtggtga cagtctttga catcagccag accgagggca aggattttcc
    19801 catgccctat atcagtgaac ttaccggcga cgtgcagcag tatcaggatt tctttgccgc
    19861 gctggaacgg acatcaccct atcccataag cattgagccg atttcagacg gggccaaggg
    19921 ccgctgcttc tacgcaaaac agcgcattgc catcaacgag ggcatgagcg aattgcagac
    19981 cctaaagacc gccatccatg agattgccca tgccaagctc cacgccattg accgggaggc
    20041 ccccgccgag gaacaggcgg cccggccaga ccgcagcacc cgcgaggtac aggccgaaag
    20101 cgtggcctat accgtctgcc agtattacgg gctggacacg tcggactatt cttttggcta
    20161 tgtggccgat tggagcagcg gcagggagtt atccgagctg aaagcctccc ttgaaaccat
    20221 ccgggccacg gccagcgaat tgataaccga gatcgacggc cattttgccc agcttcagca
    20281 ggagcgcgag gcccggcagg aacaggaggc cgtcacggtt gacgttaccc cggaggcagc
    20341 ggccccggcg caggccgagg aagaacaggc cccggccctt gatccggcag ccgagccgct
    20401 tgtgactgtg ctatggagcg aaagccccca gctaaaggac ggccagcaaa tgccgttgca
    20461 tgaggccgac gcgctgtttg cccgtctgga tgcagcatat ccggcagagg caggctatga
    20521 caaaaccgcg ttccgcattg actgtatctt tcagggcgag ccggacagct acgaggggcg
    20581 gcaggatttt ggagataggg acggttccct gatcgaacat atccagagct accaccaatt
    20641 ttacgcgcag gacgaaaact ggaaaaagca tgtaatcgct acaaccggca tagagggctg
    20701 ggagcaggac aaggcccatc gggatatgct gttaggcgag tttgtcccct atatgaagct
    20761 gcattgcagc cttgcccaaa tggaacagac ggcgcagggc cgcttacagt ccggggaaac
    20821 cctgacgccg gaggaaacgg cctatttggg ggcgcttctc tcttatgtgc aggagtgccg
    20881 ccccatgctc aatcagggcc agtatcagtt gccggagccg ccgaaactgg ctgacttcga
    20941 ccagagcttg caggactaca aagcacaggt acaggccgag atcgcgcagg aggccgccgc
    21001 agccggtatg accgtggagg aatacgcggc ggctggctat gaagcacccc aacaggacac
    21061 cttttccatc taccagcttc gaggcgggga ggaaacccgc gactatcggt ttgagccata
    21121 cgagcgttta caggccgcag ggctggccgt ggacagtgcc aactatgacc ttgtttatac
    21181 ggcaccgctg gataaaagca cgtctttgga ggatatttac cgcacattca acatcgacca
    21241 cccggcagat tttaggggac acagcctttc cgtttccgac gtggtggtgc tgcaccagaa
    21301 tggacaggac accgcccact atgtagacag cttcggctat atggaagtgc cggagttttt
    21361 gcgggaaaat cccctgcgta ccgccgaact gtctacggag cagaacgaaa acatgattga
    21421 cggcattatc aacaatacgc cgcccgaacc gcagccggag gaacccgaac agctatcttt
    21481 tgaggatgtg gcagttgctt ccggccagac acaggaggaa cccccggagg ctgcgccccc
    21541 gcaaacggct gtccgctatt atcccatcaa tgagggagcc gcccgccgcg ccaaggaagc
    21601 catgagcttt tctgactacc ggcccggcag cgccacggcg gaataccggc actatgtcga
    21661 tcaggccgtg gaaattgcgg agcgccagaa aaagcgtgtg gagccagagt atcacgaaaa
    21721 gatagacagc ctccttgaca cctacgcccg caagctggcc gagaacatga ataagggcta
    21781 tgagattacc gcccgcgtcc cctctgtcct gattgccggg ccgtccaatt tcccggtacg
    21841 ggcgaaagaa aagcagaacg ccgccagtga ccgcaacatg gaggaatttc agtacattca
    21901 gggcttgctt gacaaaatcc gcagcaccgg catgggtggt atcagcgccg acgatccgca
    21961 ggccgtttcc aagctggaaa agaaactgga aaagctggaa gcgtcgcagg agttgatgaa
    22021 ggccgtcaat gcctactacc gcaagcatgg gacgctggac ggatgccccc atctgatcga
    22081 aagaggcatt gaaaacctga aagcggatat ggcgtcgggc tggcactatg agaaaaaacc
    22141 gttccagtcc tggcagctct ccaacaataa cgccgagata cgccggttaa agggccgcat
    22201 tgaggaattg acccgccaaa aggaggcggc ctatgtggga tgggagtttg acggcggcac
    22261 tgtggagatc aaccgggtgg ccaaccgcct gcaaatcttc tttgagggga agccggatgc
    22321 tgctgtacgc gacgagctga aaagcaacgg tttccgctgg tcgcccaagg ccggggcatg
    22381 gcagcggcag ttaaacgaca ccacaatccg cgtcgctgac cgtatcaagt gcatccagcc
    22441 cctttccggc gaaaagccca gcgccttaca gcttgcggcc cgcagggaga aagaaaagcc
    22501 ctctatccgg gcgcagctca acgcggccaa gaccgaacag gccaagcagc caccggcgcg
    22561 ggaaaaatca aagggattgg aggtagagta aatgcagaag tttgaaaatg tggacgttct
    22621 ggccgcgctg gaacagatca tgcggcagaa caccgcgttt tatcaaagtg actttggcat
    22681 tgacaaagac attatcaggc gggatgccgc cagcgatcag gccgcagaca agaccctgct
    22741 ctggatgtcc cggccctccg ggacgtactg tttccgggaa cgtgatgttt tcctgaaaga
    22801 cacccgacag tacaacacat ggaaatttta cggggagcag acccgtgaca agatactggc
    22861 ctacgcggtg gagctgaccg gcacacaggg cggcactatc cggggcaatc tctatgagct
    22921 ggactatccg cagcatttcc ggcacgtcat tgaaaccgcc cagccggtga gtgtgaaccg
    22981 gctcttttat gagcatggca cccgtgacat cccggagagc cagcgttttg acggaagccc
    23041 tgaccgggta ttgggaaact ttttgtacta tgaggcccag ccccatgacc cggctgtgct
    23101 gcaggaggcg ctacggacgg agcagcacag ccgggggcgg gccgtccccg gcgatttcaa
    23161 agtccacgtc accgctttgc atgacagccg gattgaaacc gaggcccggc gtattgtgga
    23221 cagcctgaaa gatctgtccg cgcctaacag ccccaataaa acgcatttca tggtggagat
    23281 ctccccgcac tttgcggcga ttgcttccac caaggataat gaccggctct ttgccatgct
    23341 gccctataaa tccctgtgtt ttaccgggat gaaaggcagg catggcgtgt atgccgtgat
    23401 taaccgggac gagcgccggg atgtgagtat tcgcaggccc cggccctcta tccgcaagca
    23461 gctatccgac agcaaacagg ccgccagccc aaaaaaggcg gcggccagaa ccaaaaagaa
    23521 tgaattggag gtataaggaa tgatgcactt taccgtggag gaagaaaacc tgatctgtat
    23581 gtaccacaat gcagaccggc gcagaacgat aaacaggctc accgccgtca tgccggttat
    23641 ggatggggat atgcgggcgc aggcccagca gaccctttcc aagctggaac gcatgacgga
    23701 tgccgactat gacggccaga agttccactt cacagacgaa taacgaacag cagacgccgg
    23761 gcgcggcggc catgtggtcg cttgcgtccg gcgtcttttc tttttgccta ttcctacgca
    23821 tttagaaccc ggtattgtcg gggataggat gaagtattcg cttgcccctg caaacatgcg
    23881 ggagaagcct tgcagcgggc ggttttcaat cccttaaatg cgtaggttgc agctataatt
    23941 agacccctga aacagatata gccgcgcagc gggtgtatct gtttcagggg tgtgcaggtg
    24001 atagccgcaa agcggcgtaa gagggtgcag ccctcttgcc gtctatctct gttccagcgg
    24061 gaagctcggc aatgcttcca gcagtttcag ggaaatgcgc tgccgtatat cttcgtcaat
    24121 gactttcttc tttttgccgt ttgcctgtct gatctccacc gtggaaagct cgttgatgta
    24181 gtcagcataa tgggccaaca ctttttccgt ggcccacctc tcaccggcaa cggcggcttt
    24241 gatcgtttca tagtccagca gcttttcttg tccgtccatc ctgatacccc cgtcagactg
    24301 attttttcta tgataccata ttcggaagca tggaaccaga gaaaacggcg cttttctgct
    24361 ggtaaattct acgcatttag aacagcactt cggcggggat aggataaaac acccacatac
    24421 cccgctgacc atgccggaaa agccttgaaa tataagggct tttacagctc taagtgcgta
    24481 ggtttccagt atcttttccg ctggcgctcg gcctctttgc ggcggcgcat ccgtttggag
    24541 caggccgcgc agtatttggc ccggttagac ttcggcgtga acgtggcccc gcactcacag
    24601 cagcgtttca cttcgtcccg gtctttggtg atctcggcat acagggccgc atccagcggg
    24661 aggacagcgg ccttaaacca gcggcacaga agcgaatagg aaatgctctg tacgcaaata
    24721 cattcttcgc cgtcgtccag caacaggcag ttgccgtcac aatagttgca gcactcatgc
    24781 gtcagcttcc gggccttgcg atattgaggg taattcattc gtggaatact catagctctt
    24841 gcgtccattc ctttcccggc gtcatacgca aaatgccgtc cacattctgc ttgatgatgc
    24901 cgtattcctg catctgcttt ttcgcttcgc cgtacttctc atacagccgt tccttttcct
    24961 cggccaatcg cttgtactcg gctttcaggg cgtacagatc ggggagcttt ttcacaccct
    25021 gtccctttag ggcctttgcc gcagcttcat acaaaatcaa cgtgctttca tgctcacgcc
    25081 ggaattttgc cttatccgct gcattgcggt attccatagc cacgggccag agctgcttgt
    25141 aggtagaaat attctttatc agcaccgcca tatccgcaag ccggtgttcc atagctttga
    25201 gggtagcggc ggcctcgtcg ctggcggcgg tgatctcggc cactttgctt tccaaatccg
    25261 ggtaggtgtc tatcccatgt tcagtcagga aattcaaagt ctttgcggcc tgcttcaaat
    25321 tgtggagctt cgcccacctc tcatagcctg ccgactgctg ggccttaatg ttgttttcca
    25381 gatcaatccg caaagaaatc cccttgcgct ctttgggcgc tctggtgata acgcggcctt
    25441 tgatacgctc tttgatggct tcctcggtgt aggcttcccc caatgtcttg cagcgggtaa
    25501 agcgttcctg tccgggagcg cggaaagaaa taaactttcc cggcttgatc tcatagccct
    25561 gcgcctgcat gagccgcagg aaatcatcaa aatcttttgc ctgcgggata gcggcgtcaa
    25621 tggctgcttt gagcttcgct ttccagctcg tccctttccg ctgggcgtcc cactcggcat
    25681 agctcttgcc cttactctgg ccgggcttca ccacggacaa tccgttttcc ttgcacagcc
    25741 tgtcgctggt gcgccggata taggcatagc tctgtttgtt ggaaatgtac ttgcgctgat
    25801 ttaccatatc cacggcacag aaaataatgt gattgtggac gtggccctta tcaatatggg
    25861 tagtcagcac atagggatat ttcccttgca gcacttcctc ggcaagctgc ttcccgatct
    25921 catgggcctg ttccggcgtg gcttcgccgg gggcaaagga ttgtatcagg tggtggccca
    25981 gattattgcc cttttggtat gcctgcgcca gcagcatttc aaattcaatg tccgctgttt
    26041 cataagagca ggcaaaggag gccaccagca gcttgccgtc agttttgtcc gggttctcga
    26101 tgtagtcaag ggcttttttc agcgttgact tgataggatg tatctttgta actgccatat
    26161 ctgcgccagc gcccccttta tatcctcgat gtcctgtgcg tacaccggcc ccataccgtt
    26221 cagcctcttt gcaatctgat tgacgttgac gccgatcttc tgtatctcgg cggtctgcgc
    26281 ccggatgtcg ctggtgtcca tgttgacgat atagccgtca atgagcatct tccgggcgta
    26341 ggctgaaaag ttcttcgtat gcagcagcgc cattttctgt tcaatcatag cccgttcctc
    26401 cggcgtcaca gggacgcgca gcacgatttt tcgctttctg tttgccatgc ttcaccgtcc
    26461 tttccattca ggggtttggg gatttcccca acaagcaaaa cggacgcggc ccggcaggga
    26521 cggggcaggc cgtttttccg ggagtggcta cactccctat gcttgctacg ttactgtttc
    26581 tccactaaca agacgtttcc aaagccccaa actacccgca aaatagccct ctggaaagaa
    26641 aaaagtgcaa aaaaatagaa gctacgctgt gcagctcccg ctaaaagtaa aacagaggat
    26701 gccagttaca catcctccgt ttcattcttc ttcggctttt caattagtat ggcgctgtca
    26761 atcgcgccct cgatgatcgg caggtattct tcggggcaga gtttcagctt gtggctgacc
    26821 ctttgccgca gttcgctttc ctcccgcatg atctccgggt taaagtagcg ttccacagga
    26881 agcccgcagg ctttaataag ctgtatgaca accggcagac tgggaatggc ccgtcgtttt
    26941 cgatgttggc aagatagcgc cagtcaatat ttattttctc tgccattgct ttgcgcgaaa
    27001 cgcgctttgc ttgccgggcc gctttcacat ccgcgccgaa agtttcaaag ccgggacaat
    27061 cttcaatatt cgccatatcg cacctcccac ttacattgta tatttcatac tctgcccgtg
    27121 gaatgttggt atatgcacct ttcttaaagt ttatatttca cattttgttt cttttattct
    27181 tcgagcaaaa gaattataat gtaccctgtg gaggtgtact atggactaca tgacgttgaa
    27241 agaggcaggc gagaaatggg gtgtgccccc acgatggata aactactatt gtgctgcggg
    27301 acgcatcccc ggcgctgtaa agatggcgac gatctggctc attcccaaaa ctgcggaaaa
    27361 gccgattgac aggcgtacaa agaaaggaaa ggcggttact gaatagtata atgaaaccgg
    27421 cgcattttgg atatggtttt gaagagagag aaagcatggt attttgtcac ggaaaaacta
    27481 taagatgata gatggcaggc tgctccagac cgataaaaag tattctcatc tgaaacagaa
    27541 agaaaagatt tacacatgga tgtatgaaga agcgaaactt tatcatgaca gaaatggaaa
    27601 atgcccgaaa caaagacgag gaagctgctg ctgtatcagc cgtatatggt agaattgaaa
    27661 aggccggcat ctggattctt tatggtgaag tctccagaca ttggataact gctggtctaa
    27721 tgcgcctttg agtatggggt gattgatttt aataccgggg aggtacggct ttttgaggaa
    27781 aatattctgg gatttacatt tggcgattat tacatttcct gtaactgcaa ggacacggat
    27841 gatgaagata tgaggaatat agacttgctg attttctact gtcttgaacg gtattaggac
    27901 tgaatctgct gcgcggagtg cggcaagagc gaccggaggg agtacacaca ggccctgcct
    27961 gtgtgaaaag ccataccgca aggtgcattc gcaaaaaaga ggccgcgcat tttgctgagg
    28021 cttggggtgt ccccaacaag caaaacgaga acgccgcagg cgggcaagtg gtggatcaga
    28081 atatccgtaa aggatattct gatccattgc ttgccagtat tatcgggatg ttttgacaac
    28141 cgctgtaatc gaaacatccc aaacattaag aaggtgttaa ttattattat cttgatttaa
    28201 ttcgtttaaa ccaataatat agatacatac accatgaaaa gaagcgagta tcccaatgac
    28261 ggatgattaa aatccgaaaa gaaatcgctt tgaaactaca gggaaactac agactgttat
    28321 gttatcttct taaaatggag ggatttttat gtcgatacga attctacttg tcgaggatga
    28381 tgatcatatc tgtaatacag taagggcgtt tctggctgag gcaggatatc aggtggatgc
    28441 ctgcacagat ggaaatgagg catacaccaa gttttacgaa aacacttatc aactggttat
    28501 tcttgatatt atgctgcccg gtatgaacgg gcatgaactt ttgcgtgaat ttcgtgcgaa
    28561 aaatgatact cccattctga tgatgacagc cctgtcggat gacgaaaacc aaatccgggc
    28621 gtttgatgca gaggcagacg actatgtaac aaagccattc aagatgcaga ttttactaaa
    28681 gcgggtggaa gccctgctgc ggcgcagcgg tgcgctggca aaggaaatcc gtgtcggcag
    28741 gctgacactt ctgccggagg atttcacggt actttgtgac ggtacagagc tgcccctgac
    28801 acgaaaagaa tttgaaatcc ttttgctgct ggtgcagaac aaaggcagaa ccttaacgca
    28861 tgaaatcatt ttgtcacgta tatggggata tgactttgag ggtgatggca gcacagtcca
    28921 cactcatatc aaaaatttgc gggccaagct gccggaaaat atcatcaaaa ccattcgcgg
    28981 tgtaggatac cgattggagg aatcataata atggaaagaa aagggatttt cattaaggtt
    29041 ttttcctata cgatcattgt cctgctactg cttgtcggtg taacagcaac actgtttgca
    29101 cagcaatttg tgtcttattt cagagtgatg gaattacagc aaacagtaaa atcctatcag
    29161 ccattggtgg aactgattca gaatagcgat aggcttgata ttcaagaggt ggcagggctg
    29221 tttcactaca ataaccaatc ctttgagttt tatattgaag ataaagaggg aagcgtactc
    29281 tatgccacac caaatgccaa tacatcaaat agttttagac ccaactttct ttatgtggta
    29341 catagagatg ataatatttc gattgttgct caaagcaagg caggtgtggg attgctttat
    29401 caagggctga caattcgggg aattgttatg attgcgataa tggttgtatt cagcctttta
    29461 tgcgcgtata tctttgcgcg gcaaatgaca acgccgatca aagccttagc ggacagtgcg
    29521 aataaaatgg caaacctgaa agatgtaccg ccgccgctgg agcgaaagga tgagcttggc
    29581 gcactggctc atgatatgca ttccatgtat gtcaggctga aagaaaccat cgcaaggctg
    29641 gaggatgaaa tcgcaaggga acatgagttg gaggaaacac agcgatattt ctttgcggca
    29701 gcttctcatg agctaaaaac gcccatcgcg gctacaagcg ttctgttgga gggaatgctt
    29761 gaaaatatcg gtgactacaa agatcattct aagtatctgc gcgaatgcat caaaatgatg
    29821 gataggcagg gcaaaatcat ttccgaaata ctggagcttg tcagcctgaa tgatgggaga
    29881 atcgtaccca tagctgaacc gttggacata gggcgcacgg ttgccgagtt gctgcccgat
    29941 tttcaaacct tggcagaggc aaacaaccag cggttcgtca cagatattcc agccgggcaa
    30001 attgtcctgt ccgatccgag gctgctccaa aaggcactat ccaatgtcat attgaatgca
    30061 gttcagaaca cgccgcaggg aggcgaggta cggatatgga gtgagcctgg tgctgaaaaa
    30121 tgccgccttt ttgttttgaa catgggcgtt cacattgatg atactgcgct tccaaggctg
    30181 ttcaccccat tctatcgcat tgatcaggcg cgaagcagaa aaagtgggcg aagcagaaaa
    30241 agtgggcgaa gcggtttagg acttgccatc gtacaaaaaa cgctggatgc catgagcctc
    30301 cagtatgcgc tggaaaacac ctcggatggc gttttgttct ggctggattt accgctcaca
    30361 tcaacattgt aaatatttaa aacttaaatg attttgggct ctttgtcaaa taggactgat
    30421 agagtgagat aagaacatct aaggtagaat tccttttcta ctttagatgt tttttgattc
    30481 ataggtaatc agattattta gtaagaaaat tctggtcttc attttccgaa agcttcgaaa
    30541 gccataagcg actcgtttca atgctttgat gtgggtatgc agattttcta ttttggcatt
    30601 tgaataaggc aattcgaggg ctaaacgaat gccggattca tagtttataa aagctttttt
    30661 tatagaatgc agttcttttg gaacggaatt gggcatcgtt cttacaagtt taaagaaagt
    30721 gtccgcctct ttgtcgtgaa acgcttgaag aatttcttga tagtaaaaat aggcgattcg
    30781 taattcgtca gagatcgaaa gcagacgatc caccacttca ctttcggtga gatacgccca
    30841 accaaaattg cgccatttgg tccatttgtc ataaacaagc ttgtcctgtc gtttgatcag
    30901 aagccgagac aacgccttta attgtcgata atatttggct tgtgttggat cgtttcgatc
    30961 aaagcttttc atgattcgta cgcgaatatc ctcaaattta cgaatgagat gtttgacaat
    31021 gtgaaaacga tccacaacga gttttgcctt tggaaaacat tcctttatca gagaggcata
    31081 agaaacattc atgtccgtca cgatgacctt taccttcaat cgtgcgcgtc gttcaaattg
    31141 cataaaataa gaaacgagag cctttttctt ccgtgaagga agaatatcca gtaattttcc
    31201 agtatcgcca tccatacaag aaaaggagta gggaccgact tgatttttca gtgaacgaaa
    31261 ttcatccatt aataaacaac gcggtaacca atttcttttt acttttgtca tggggatcag
    31321 agaagcgagt gtccgagaaa cggtcgacca agaaacgccg caatgcttag caattgtcgt
    31381 cattgcttgt ttttcagtta gctcttctaa aattttatag cgcacttgtt tagcgataaa
    31441 gcagtttcta tcgacaatgt aggtattcgc cgtaaaatag gtgtggcagc cacgacagtg
    31501 gtagcgttgt ttcgctaaag caagataagt cagttggttc gcacatttat taagttgaat
    31561 gagcgatttt ttctttccgt tcttaacgat catgccttca tgattcatct gtttacacaa
    31621 aggacagcga cgaatttctt tacttaacgt cgcagaaaca acaagactct tgatattctt
    31681 aatgctcttc gattccacat ttgtgatgaa aatgttttta tctttgattc caagtagtag
    31741 tttgatatga tgaagatcag gcatacagaa cgctcctttg attgtgttgt ggtgattcaa
    31801 ttctaacgga gcttctgtat gttttgtttt ttcatgctca aaagggaccg atagaatgat
    31861 tttctatcag tcctagaaat tatagagccg attttgaccg acaggtataa ccctgccggt
    31921 ctttttttgc tgctacagga aaactacaga ctggctacag ggaaagtaca gatgcgcttg
    31981 ccataataac aatcgtacca gccacaaatc gtagttttat tgcaaaggag gcattcgatc
    32041 aaatggaaaa aagcaactat cattccaatg cggatcatca caaacggcat atgaaacaat
    32101 ccgtggaaaa gcgggctttt ctatgtgcgt tcattatttc gttcacagtc tgcacgctgt
    32161 ttttggggtg gagactggct tccgtattgg aggcaacaca gataccgccc atccctgcaa
    32221 ctcatacagg cagcagcact gacgtagtgg agaatttgga ggaaaacgct cttgccaccg
    32281 ccaaagaaca gggagatgaa caggaatgga gcctgatttt agtgaacagg cagaacccca
    32341 tccccgcaca gtacgatgtg gagcttgagc aactatcaaa tggtgagcgg atagatattc
    32401 ggatttctcc ctatcttcaa gatttgtttg atgccgcaag aactgatgga gtttacccga
    32461 ttgtcgcatc cggataccga acaacagaaa aacagcaaga aattatggat gaaaaaattg
    32521 ccgaatataa ggcgaaaggc tacacctctg cacaggctaa agcggaagca gaaacttggg
    32581 tggccgtgcc gggaacgagc gagcatcagc ttggtcttgc tgtggatatc aatgcggacg
    32641 gaattcattc aacaggcaac gaggtttata gatggctgga tgaaaacagc tatcgttttg
    32701 gttttattcg tcgctaccca ccagacaaga cagagataac cggtgtgagc aacgagccgt
    32761 ggcattaccg atatgttggc atcgaagctg ccacagagat gtacaaccaa ggggtttgcc
    32821 ttgaggaata tttaaaacca gaaaaatgag aaaaggatgt tatgttatgg acagaaaaag
    32881 attgacacag cgtttcccgt tcctgcttcc aatgagacgg gcgcagagaa aaatgtgctt
    32941 ttatgcggga atgagatttg acggctgtcg ctatgcgcag acgatagggg aaaaatcgct
    33001 ttcccatttg ctctttgaaa cggattgcgc attatacaac cacaataccg gatttgacat
    33061 gatataccag gaaaacaagg tgttcaactt aaagctggca gcaaagacct taaacggctt
    33121 attgataaga ccgggggaaa ccttttcttt ctggttgttc ctgcatacgc cattgacaat
    33181 tatccagcgc agaggtcacg aagtaaagga gtttccagag ccaaacagtg acgagattaa
    33241 aggggtggat gcaaccattt cagagggctg gattgattta aaagtgcgaa acgataccga
    33301 ctgcacctac caaatatggg tgaccttaga tgatgagaaa atcatcggtc aggtgttcgc
    33361 cgacaaagag ccccaagcat tatacaaaat tacaaatggc agtatccagt atgtccgtga
    33421 aagtggcggg atttatgaat atgcccaggt taaacggatg caagttgcct taggtaccgg
    33481 ggaaataata gattgcaagc tgctttatac aaacaaatgc aaaatctgtt atcccctccc
    33541 ggaaagtgtg gatattcagg aggagaacca atgagaaaaa gtgtgggcat tactgttttt
    33601 ggatgcgaac aggatgaggc aaatgctttc cgcgctttat cgccggattt tcatattatc
    33661 cctacgctga ttagcgatgc gatatcggca gacaacgcaa aattggccgc tggcaatcaa
    33721 tgcgttagcg taagccataa gtccgaggtt tccgaggcga caattcttgc gctgagaaag
    33781 gtcggggtaa aatacatttc tacccgcagc atcggctgcg atcacattga tacgactgcc
    33841 gccgagagaa tgggaatctc ggttggcacg gttgcgtatt cgccggacag cgttgcggat
    33901 tatgctttga tgctgatgct gatggccata cggggtgcaa aacccaccat gcacgccgtg
    33961 gcgcaacaag atttcagatt ggatcgtatc cgggggaaag aactggggga tatgactgtg
    34021 ggagttattg gaaccggcca tatcgggcaa gcggtcgtca aaaggctgcg gggatttgga
    34081 tgccatgtgc tggcctatga taacagccga aaaatggatg cagattatgt ccagcttgat
    34141 gagcttctaa aaaacagcga tattgttacg ctccatgtgc cgctttgtgc ggatacccgc
    34201 catctgatcg gtcagaagca aattggagag atgaagcaag gcgcattttt aatcaacacc
    34261 gggcgcggag cgcttgtcga taccggggcg ctggtggagg cgttggaaag cggaaagctg
    34321 ggtggtgcgg cactggatgt gttggagggc gaggatcagt ttgtttatac cgattgctcg
    34381 cagaaagtgc ttgaccatcc ctttttgtcg cagcttttaa ggatgccgaa tgtgatcatc
    34441 acaccccata cggcgtacta cactgagcgt gtgctgcagg atactacaga aaaaacaatc
    34501 aggaattgtc tcaattttga aaggagttta cagcatgaat agaataaaag tcgcaatcat
    34561 cttcggcggt tgctcggagg aacatgatgt gtcggtaaaa gccgcaatag aaattgctgc
    34621 gaacattgat acggaaaaat tcgatccgca ctacatcgga attacaaaaa acggtgtatg
    34681 gaagctatgc aagaagccat gtacggaatg ggaagccgac agtctccccg ccatactctc
    34741 cccggatagg aaaacgcatg ggctgcttgt catgaaagaa agcgaatacg aaacacggcg
    34801 tattgatgtg gctttcccgg ttttgcatgg caaatgcggg gaggatggtg cgatacaggg
    34861 gctgtttgta ttgtctggta tcccctatgt gggctgtgat attcaaagct ccgcagcttg
    34921 catggacaaa tcactggcct acattcttac aaaaaatgcg ggcatcgccg ttcccgaatt
    34981 tcaaatgatt gataaaggtg acaagccgga ggcgggtgcg cttacctacc ctgtctttgt
    35041 gaagccggca cggtcaggtt cgtcctttgg cgtaaccaaa gtaaacggta cggaagaact
    35101 taacgctgcg atagaagcgg caggacaata tgatggaaaa atcttaattg agcaagcgat
    35161 ttcgggctgt gaggtcgggt gtgcggtcat ggggaacgag gatgatttga ttgtcggcga
    35221 agtggatcaa atccggctga gccacggtat cttccgcatc catcaggaaa acgagccgga
    35281 aaaaggctca gaaaatgcga tgattacagt tcccgcagac attccggtcg aggaacgaaa
    35341 tcgggtgcag gaaacggcaa agaaagtata tcgggtgctt ggatgcagag ggcttgcccg
    35401 tgttgatctt tttttgcagg aggatggcgg catcgttcta aatgaggtca ataccctgcc
    35461 cggttttaca tcgtacagcc gctacccacg tatgatggcc gccgcaggaa tcacgcttcc
    35521 tgcactgatt gacagcctga ttacattggc gttaaagagg tgataagtat ggaaaatggt
    35581 tttttgtttt tagatgaaat gttgcatggc gtccgttggg atgccaagta cgccacatgg
    35641 gataacttca caggaaaacc ggtggatggg tatgaggtga atcgcatcat cggcacaaag
    35701 gccgtggcgt ttgctctgcg cgaggcacaa atccatgcgg ctgcgcttgg ctatggcttg
    35761 cttttatggg atggatatcg gccaagaact gcggtggact gcttcctgcg ttgggcagcg
    35821 caaccggagg acaatctcac aaaagaaaaa ttttacccca atatagagcg agccgagttg
    35881 attacaaagg gttatgtggc ctcacaatcc agccatagcc gtggaagcgc aattgatctt
    35941 acgctctacc acctggatac aggggaactt gtttcaatgg gaagtaactt cgattttatg
    36001 gacgaacggt cgcatcatac agcaaaaggg ataggggatg cagaggcaca aaatcgaaga
    36061 tgcttgcgta aaatcatgga aagcagcgga tttcagtctt atcgctttga atggtggcac
    36121 tataagttga ttgatgagcc ataccccgat acctatttta attttgccgt ttcataatga
    36181 aagtattggt tttgtatcag attttggatt tgataaaatc tatgctggtc atggacaatc
    36241 ttttactgga aagtggttaa agcatctttt gtaatacatt gcaaattata gtattcaatc
    36301 tgcccagcgg cagaaaagaa aaaaaccgct gttgggcaga cctttcccac gcccattttg
    36361 tattttgctg gcggcggttt tgagaaatca aggccgccgt tcttttctgg cagcaaaagg
    36421 tgtggcggcc cataggagga tgccgctatg ctgttttgct attgccatgc tctaatccac
    36481 ttccaccact caaaaaaagc cttgcccctc
//
LOCUS       DAZBTF010000020        36462 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000020 DAZBTF010000000
VERSION     DAZBTF010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.020"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..3654
                     /locus_tag="ACHGNS_001098"
     CDS             127..3654
                     /locus_tag="ACHGNS_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFN6937059.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="ACHGNS_001099"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="ACHGNS_001099"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFN6937060.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="ACHGNS_001100"
     CDS             7442..7783
                     /locus_tag="ACHGNS_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFN6937061.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="ACHGNS_001101"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="ACHGNS_001101"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFN6937062.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /gene="pheT"
                     /locus_tag="ACHGNS_001102"
     CDS             9140..11560
                     /gene="pheT"
                     /locus_tag="ACHGNS_001102"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFN6937063.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="ACHGNS_001103"
     CDS             complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="ACHGNS_001103"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFN6937064.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12994..13731
                     /locus_tag="ACHGNS_001104"
     CDS             12994..13731
                     /locus_tag="ACHGNS_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFN6937065.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13745..14560
                     /locus_tag="ACHGNS_001105"
     CDS             13745..14560
                     /locus_tag="ACHGNS_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFN6937066.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14573..15220
                     /locus_tag="ACHGNS_001106"
     CDS             14573..15220
                     /locus_tag="ACHGNS_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFN6937067.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15236..15892
                     /locus_tag="ACHGNS_001107"
     CDS             15236..15892
                     /locus_tag="ACHGNS_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFN6937068.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16054..16875
                     /gene="racE"
                     /locus_tag="ACHGNS_001108"
     CDS             16054..16875
                     /gene="racE"
                     /locus_tag="ACHGNS_001108"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFN6937069.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16878..18233
                     /gene="rph"
                     /locus_tag="ACHGNS_001109"
     CDS             16878..18233
                     /gene="rph"
                     /locus_tag="ACHGNS_001109"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFN6937070.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18234..18752
                     /locus_tag="ACHGNS_001110"
     CDS             18234..18752
                     /locus_tag="ACHGNS_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFN6937071.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18859..19359
                     /gene="cbpB"
                     /locus_tag="ACHGNS_001111"
     CDS             18859..19359
                     /gene="cbpB"
                     /locus_tag="ACHGNS_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFN6937072.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19639..20559)
                     /locus_tag="ACHGNS_001112"
     CDS             complement(19639..20559)
                     /locus_tag="ACHGNS_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFN6937073.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20685..21737
                     /locus_tag="ACHGNS_001113"
     CDS             20685..21737
                     /locus_tag="ACHGNS_001113"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFN6937074.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            complement(21847..22602)
                     /gene="fabI"
                     /locus_tag="ACHGNS_001114"
     CDS             complement(21847..22602)
                     /gene="fabI"
                     /locus_tag="ACHGNS_001114"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFN6937075.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22822..23262
                     /gene="fabZ"
                     /locus_tag="ACHGNS_001115"
     CDS             22822..23262
                     /gene="fabZ"
                     /locus_tag="ACHGNS_001115"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFN6937076.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23377..24138)
                     /locus_tag="ACHGNS_001116"
     CDS             complement(23377..24138)
                     /locus_tag="ACHGNS_001116"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFN6937077.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24327..25421
                     /locus_tag="ACHGNS_001117"
     CDS             24327..25421
                     /locus_tag="ACHGNS_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFN6937078.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            complement(25507..26466)
                     /locus_tag="ACHGNS_001118"
     CDS             complement(25507..26466)
                     /locus_tag="ACHGNS_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFN6937079.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            26652..27587
                     /locus_tag="ACHGNS_001119"
     CDS             26652..27587
                     /locus_tag="ACHGNS_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN6937080.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            complement(27672..28682)
                     /gene="gap"
                     /locus_tag="ACHGNS_001120"
     CDS             complement(27672..28682)
                     /gene="gap"
                     /locus_tag="ACHGNS_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFN6937081.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            28970..30283
                     /gene="obgE"
                     /locus_tag="ACHGNS_001121"
     CDS             28970..30283
                     /gene="obgE"
                     /locus_tag="ACHGNS_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFN6937082.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            30504..31091
                     /locus_tag="ACHGNS_001122"
     CDS             30504..31091
                     /locus_tag="ACHGNS_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFN6937083.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            31268..32209
                     /gene="rnz"
                     /locus_tag="ACHGNS_001123"
     CDS             31268..32209
                     /gene="rnz"
                     /locus_tag="ACHGNS_001123"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFN6937084.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            32206..32994
                     /locus_tag="ACHGNS_001124"
     CDS             32206..32994
                     /locus_tag="ACHGNS_001124"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFN6937085.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            32995..33438
                     /locus_tag="ACHGNS_001125"
     CDS             32995..33438
                     /locus_tag="ACHGNS_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFN6937086.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            33580..35877
                     /gene="recJ"
                     /locus_tag="ACHGNS_001126"
     CDS             33580..35877
                     /gene="recJ"
                     /locus_tag="ACHGNS_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFN6937087.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            35891..36403
                     /locus_tag="ACHGNS_001127"
     CDS             35891..36403
                     /locus_tag="ACHGNS_001127"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFN6937088.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
BASE COUNT        11947 a         6322 c         7842 g        10351 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aaggaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
    11641 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
    11701 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
    11761 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
    11821 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
    11881 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
    11941 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
    12001 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
    12061 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
    12121 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
    12181 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
    12241 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
    12301 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
    12361 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
    12421 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
    12481 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
    12541 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
    12601 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
    12661 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
    12721 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
    12781 tatcttaccg tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg
    12841 ataaaataac taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag
    12901 aaactttaca aattactaaa ctatcagata taatgtattt tattatacat ctttaaaaga
    12961 aatcttacta aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg
    13021 gataaatatt atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga
    13081 gaagttgtcg ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc
    13141 aatggattag agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat
    13201 aaaaagacaa aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat
    13261 ttgtacccaa ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag
    13321 caagaccagg caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta
    13381 gataagaaag attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc
    13441 gccagaggtt tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg
    13501 gatccggaaa tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg
    13561 tcaatgattg tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc
    13621 ttcatggctg atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat
    13681 ccaaatgaag tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa
    13741 aaagatgcgc aggttaaaaa aaatacgcct gattttcctt ttaggcattg cttttcttgc
    13801 actagccgga tgtaaaggaa atgggttggc aaatgaagat attttagagc gaagcaaatc
    13861 taccaatgaa atcatctggg gtgtcaagta tgatactcga ttatttggaa tgatggatat
    13921 tgagagcaga actgtacaag gatttgatgt agatattgca aaagcaatca ctaaaaagat
    13981 ccttggtgac aatggaaaaa cagaatttgt tgaagtcact tctaaaactc ggattccatt
    14041 attgaaaaac ggaaatatcg atgcaattat tgcaacgatg acgatcacag atgaacgaaa
    14101 aaaacaggtt gacttttcag atgtctattt cgatgctggc caagctttgt tggtaaagaa
    14161 aggaagccaa atcaaaagtg tagatgattt gaatgcttct acaacggttc tagcagtcaa
    14221 aggttctaca tcggctgcga atatccgaca gcatgcacca gatgcaaaaa ttttagaatt
    14281 agaaaactat gcagaagcat ttactgcttt acaatctggt cagggagacg cgatgactac
    14341 ggataatgcg attttgttag ggattgcaga tgaaaatcca gagtatgagt tagttggtgg
    14401 aacgtttact aatgagcctt acgggattgc gatcaacaaa gggcaagaaa acttcttgaa
    14461 agctgttaac caagcgctag aggaaatgca tgcagatggc acatacgata aaatctatca
    14521 aaaatggttt ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg caatgagcga
    14581 attgatacaa acttatggac caatctttgt cgacgggttc aagatcacat tgttttcgag
    14641 tgtcttagca cttgtgttta gtttgattat cggaacgtta atggcaatct tccaattatc
    14701 aaagaataaa gtagtgagcg ccttagcaaa ggcatatgta gaatttttta gaaacattcc
    14761 attattaatc atcgtgatgt ttttttatgt tgtcgtaccg atttattggt ttaatctaga
    14821 tggattccaa gcaggtacga ttggattaac gatctataca tctgctttta ttgctgaaac
    14881 agtacgttca gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg
    14941 ttttacctat tcagaaatca tgcgttatat tgttttaccg caagcattca aaatcgttat
    15001 cccgccatta ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt
    15061 agctggttta gatttgatgt atcaaggaga cttgatcgct agtgaaacat tcaatacctt
    15121 cgatacgtat atcatcgttg ggttatttta cttagttatc acgctaccgt tatcttactt
    15181 aatggtttat cttgagaaaa aatgggcggt tcgttcatag aaggagggga aatacatgga
    15241 ttttagcggc gctttttcat ggatgaatat ccgtttttta ttggaaggat taaaggttac
    15301 tgttgaagtt tcattgtttt cgatcatttt tagtttcttg atcggtggtc tgacaggtgt
    15361 gattcgtttt gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga ttgatattat
    15421 acggaatctt ccattattat tgatcatttt cttcacttac tttgccttac ctcaaatcgg
    15481 cattcagatg aacatcttct ggtccgctgt cgcagcatta acgatttttg agtcagctat
    15541 gttgtcggaa attttccgag ctggactcaa tgctgttcca aaaggacaga tggaagcagg
    15601 attatcaacg ggactgactt atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt
    15661 taaatcaatg atcccggcaa ttgtgagcca attgatttca ctgatcaaag atacttcatt
    15721 agcagtaatc atttctttac ctgaactttc tcatcaggca cgtattattt atggacagaa
    15781 caccaattat gtgcttccga tgttcatcat catgacactt atgtacttcg tggtatgtta
    15841 cgcgctttct ctgctttccg gttacttgga aaagagacgg tacagttatt aatagatgat
    15901 tcttttagcg gttgtggcaa actaaagtca caaccgcttt tcctctttcc attcgaaaaa
    15961 aagagatttc gattgtttta agcaagtaag agcacttgaa aattcacgct aaaaagtaca
    16021 caatagggag gatagaattt attgaaaaaa aggatgatac gattgacaga taatcgccct
    16081 atcggattta ttgattcagg tgtcggcggc ttgactgtag taaaagaagc cctgaaacaa
    16141 ttaccgaatg aaaatatttt atttgtagga gacacagcac gctgcccata tggccctaga
    16201 cccgcggaac aggtaatcca gtatacttgg gaaatgacgg attatctggt ggagcaagga
    16261 atcaagatgc tggtgatcgc ctgcaatacc gcaactgcgg tggctttaga agaaatcaaa
    16321 gctgctcttt ctattccagt catcggtgtg atccttcccg gtactagagc ggcagtaaaa
    16381 aaaacacaaa ataaacaagt tggcattatc ggtacgattg gtacggtaaa aagtcaagct
    16441 tatgaaaaag cactgaaaga gaaagtacca gaattgactg tgacaagtct tgcttgtccc
    16501 aaatttgttt cagttgtcga aagtaatgaa taccattcat cggtggcgaa aaaaattgtg
    16561 gcagaaacat tagctccttt aaccactaaa aaaatcgata cattgatttt gggatgcacc
    16621 cattatccat tattacgccc catcattcaa aatgtaatgg gagaaaatgt tcaactgatc
    16681 gattctggag cagaaacagt aggtgaagta tctatgctgt tagattattt caatttgagc
    16741 aattcaccgc aaaatggtcg gacattatgc cagttttata caactggctc tgccaaactt
    16801 ttcgaggaaa tagctgaaga ctggcttgga atcggacact taaatgtaga acatatcgaa
    16861 ttgggaggaa aataaggatg cgtcatgacg ggagaaaagc ccaagagctt cgcaaactgg
    16921 agcttcaaac aaatgttttc aaatatccag aaggatcggt agtgatccgt tttggtgata
    16981 caacagttat ttgttctgca acaatagaaa attctgttcc cccttttttg agagaaacag
    17041 gaacaggatg ggtgacagca gaatatagta tgctgcctcg agcaacgagt acgagaaatc
    17101 gtagagaaag cagcaaaggg aaactttctg gtagaacaat ggaaatccag cgtcttattg
    17161 gtcgttcctt gcgtgcagta gtagacttgg aaaaattagg agaacgtagt atcatcgtag
    17221 actgcgatgt gatccaagcg gatggcggta ctagaacagc aagtatcact ggggcatttg
    17281 tggcattgaa attagccatt gaaaaacttt taagagaaaa agaattgtca gaagatccaa
    17341 taaaagaaca tcttgctgca gttagcgtag gaatcttacc agatgggact tgcgtcacag
    17401 atttggatta ccaagaagat tccgcagcac ttgtagacat gaatcttgta atgacagaat
    17461 ctggaaaatt cgtggagatt caaggaactg gagaagaagc aacgtttgat ggtgaacagt
    17521 tgaatgaaat gctctttttt ggaaaaaacg cgattgaaga tttgatcaaa gagcaaaaac
    17581 atgctttgct aacagagttt gctcaaaatg atgagcggat agaagaaaca aaaacaatca
    17641 tcattgcgac aagaaatcca ggaaaagcag aagagtttcg aaatatgttc aaagaagcgg
    17701 gatatcatgt aaaaacatta cttgactatc ctgaattgcc agatgtggaa gaaactggca
    17761 gcacatttga agaaaatgcc cgattaaaag cagaaacgat tgcacaattg cttgatcagc
    17821 cagtcttagc agatgattca ggtttgaaag tggatgcttt aggcggtatg ccaggaatct
    17881 attctgcacg ttttgcaggg gaacaaaaaa gtgatgcggg gaataacgcc aaattattat
    17941 atgaattgac agatgtgccg gatgaaaaaa gaacagcaca attccattgt acgttagtat
    18001 tcgcggcacc caaaaaagac agccttgtag tagaagcgga atggcccggt cgagtagccc
    18061 gtataccatc tggcgaaaat ggctttgggt atgatccttt atttatccca gaaggtaaga
    18121 aacaaacagc agcagaatta tcgagcgaag aaaaaaacaa gatcagtcat cgggctcaag
    18181 caatgaaaaa attgagtgcc gagtggaaac aatggctgga aggagagaga tagatgcggt
    18241 atttagtagt aagtgataat catggagaca gagatatcct cgttgactta gtagatcgtt
    18301 atcgttctga agtggatatc tttttccatt gtggtgattc ggaattagaa gcttctgacc
    18361 cattatggga tacattccaa gtggttcaag gtaattgtga ttatggacca ggattcgaac
    18421 agaaaaaagt gatccaaact ggtcaggata cagtattcat gactcacggt catttatcga
    18481 atgtccgatt tggtttgaca cagttagcaa tcgaagctcg agctgcaact gccgatatgg
    18541 tgttttttgg tcatacccat caaataggct gtgagatgga tcagaaaatc ctgtttttga
    18601 atcctggtag catttctcag cctagaggcc cgatccaaat cccatcatat gctcttatcg
    18661 aaagtttaga tgaccaaatt tctgttcaat actataaccg tgcccatcag gcagttgaag
    18721 agttggcctt ttctttttcg agagagcgct gaataaaaaa actctaattt ttaataaaaa
    18781 taagctttat tttatgaatt ttgcatccgg tttcggtaaa atagtagtgt tgaatgaaat
    18841 agaaatggag gccatcagat gattggacct atagtaagag aaatgctgct ggaaaatcag
    18901 gaaacgtttt tgattcctgc agaaaatgtg gcaaatgtta tgtatcatca tccattgtcc
    18961 catggtttac ttgtgttatc gaaggtcggt tattccaaaa ttcctgtatt aggaaaagat
    19021 gatcactttg taggactagt aagtttatca gatgtggtca ataaaatgat tgacttgcaa
    19081 acaatcagca tggacccgtt agaaggacta acggttgctg atgtgacaga aacagatgtt
    19141 ccgacaattg atgaaaattg ggaactagaa gaagtactcc atttgttagt cgattcgcct
    19201 tttttaccgg ttgtcacaga agatcggatt ttcaaaggaa tcattacaag aaaagagctg
    19261 cttaaagccg taaacttcat ggtgcatgaa ttagaacgtc gtaatatatt gtctccgaaa
    19321 atcgaagaat taaaagaaca aatcgatatc gtgagctaaa gaacgagaaa ctaaaagata
    19381 atcaaaaaat aagtagaaaa aacagcgtac cagttttgac tttatcttga aaaagaggct
    19441 gggacaaaag cgtttaactc caagaaataa gaaggaattc acgaaaattg cttttcaatt
    19501 ttttgtgaat ttcagcttat ttccgaagga gttgcttctg cttccgccgt ttatacgtgt
    19561 ttagagcgtg ggacaaaagt gttctttact tttgtcccac gctcttttta gtgagattcg
    19621 ttgcatcgtt tgtccaaatt attttccgac agcaattgga ctgttcggaa tcgtgaaacc
    19681 agaagctgtc aattctttga cataggagct caagaactct tcttttaagg catattgttt
    19741 tccgttcaat gcgtagcaga ccgtacgaat cgcaaaattg ctgtttccaa gatccactaa
    19801 tccaaagata gaaggttcag tttggagctc ttcttggtat ttttcagcta atgtacgatt
    19861 tactcgatca atgatctcat agattttgtc atatccttct tcaggaacaa tccgaatatc
    19921 taaaagtact tgcatatttg cacgagagag attgctgatc gtggtgatgt ttcgattcgg
    19981 gatgaaatgg acagtaccat ccgttgcttt cagctgaagg gtacggatac caacagatac
    20041 gacggttcct tcaatcgtta agtttgctag cttgatgtag tccccaacat ccatctgttg
    20101 ttccatgata ataaaaaatc cggtaataac atcactcatg aacccttggg caccaagacc
    20161 gattgccaca cctgcaattc cggcaccagc caaaagtgat ccgatcggca caccgatagc
    20221 agataaaatt gcatagataa aaaagaaacc aagggtataa tgaaaaatag tatttaaaag
    20281 actatgtaaa gtctttaagc ggctctcgtt tagaatggct tttttagaat actgattaaa
    20341 agacttatca atcagatatt ttcccacacg gtgaagaatc caaaaaagga cgatcaaaaa
    20401 aatcagatat aaactttttt ggataaaggc agccaaaatg gcgtcccaat caatcccgtc
    20461 ccaaaaccgc tgaaacgcat ttactttgct gacagcttga tctgtcaatg tctcgccaga
    20521 agagtctgtc gtaccggcag ttgctaataa tgaaaacata aaatcctctt ttctaaaatt
    20581 tgattctttc tgtattgtac caagaagttt tcaaaaaagg aaatagcaaa aataactata
    20641 atgaaggaaa aaagctgaaa ataaaatgac aaataatatg gataatgata aaatgtggag
    20701 aaaataacga aggtggtaag tatatgcaaa tcgattggaa caatctaggt ttttcttata
    20761 tcaaaactcc ttggcgtttt attgccaaat ggaaagacgg acaatgggaa gaaggagaat
    20821 tgacagaaga taattacctg acgattcatg aaggctctcc agcgcttcat tatggtcagc
    20881 aatgttttga aggtctaaaa gcttatcgtc gaaaagacgg caaggtcaat ctctttcgac
    20941 ctgaacaaaa tagtcgacgg atgaatcaaa gtgctagacg attgctgatg ccaatggtcc
    21001 ctgaaaagat gttcgttgat gctgtcaagc aagtggtaaa agcaaatgaa gattttgtgc
    21061 cgccctatgg aacaggcgct acattatact tgcgcccttt actgattggg gtcggtgaaa
    21121 acattggtgt ccatccagct ccagaatatt tatttgtcgt cttctgtacg ccagtaggag
    21181 cgtatttcaa aggtggacta aaaccaacga attttattgt atctgattac gatcgtgctg
    21241 ctcctaaggg gactggtgca gcaaaagtcg gcggaaatta cgcagcgagt ttacttccag
    21301 gaacagaagc aaaagaactg gattactcag attgtgttta tcttgatcca gccacacata
    21361 cgaaaatcga agaagtaggt gcagcaaatt tctttggaat tacaaaagac ggaaaattca
    21421 tcactccgca atcgccctct atcttaccaa gtatcacgaa atattccttg ttgcagttag
    21481 ctgaagaacg actaggactg caagcaattg aaggagatat tttcattgat gatctagatc
    21541 aatttgcaga agctggtgca tgtggtacag cggctgttat ttctccaatc ggtgggattt
    21601 atcacaataa caatcttcat gtattttata gtgaaacgga agtaggacca gtaacaaaac
    21661 ggttgtatga tgaattgaca ggtattcaat ttggagacat agaagcacca gaaggctgga
    21721 ttgaagtcgt ggaataattt caaagtaatg agttgaaaaa aacgtaaaaa gaaaaaatca
    21781 ggaagagact ttaaaaagtt ttttcctgat ttttatatgt tttactttaa agaatgcaac
    21841 aaaagtttat gaaagatgga cacctttgtc aacgtagata atatcaccga tgattccgct
    21901 ggaaagtggg ctgattaaga aagcacaagc attaccgact tcttcgatcg tcacaccttg
    21961 ctgatcaggc gtacgagatt cagataactg gatcagtttt tgatagtctt taacacctgt
    22021 gacagctaat gttttgattg ccccagctga gattccattg acccgaattc ctttaggcga
    22081 gaactcagct gccaaataac ggatagcagc ttcaagagaa gctttcgcaa tacccatcat
    22141 gttataattc gggattgcgc gttctgaacc tagataagtc atgctaacga tcccgctgtt
    22201 tggtgcaaga aacggttgtg cgtattttga tacagcaatt aaagagtagc tgctgatatc
    22261 ttgagctaat tgatagccgt ctctggaaat atcagacaca tttccggaca attcttcttt
    22321 gttggcgtaa gcaactgcat ggacaaggcc gtggatctcc ccagcatatt caccgatggc
    22381 attcattgct ttttcgatgc tttcgtcgct agaaacatca cattctacag tgaatgcttc
    22441 ttcgccgacc aatttgacta atgatttttt cattcgttca ttttggtaag tatagataat
    22501 ttctgcccct tgtttcaaca ttgcttcagc acaaccccaa gcaatgcttt ttttgttagc
    22561 gacgcccata atgacgattt tttttccttc tagaaatgac ataatgactc tcctttgttt
    22621 ctgataaaaa tttgatagtc gtaaaaaaat agagaataat agctaaaata acttcttgat
    22681 ttcttaatta ttagtcaaaa tgttttgatt gtcaaactat tttttcttaa attaaaaaaa
    22741 gattgctaaa tatttaggaa tatgatactt tgatagtcaa agtaatgaaa atggcaattg
    22801 cctgaaagaa ggagtttaat tatgtcttac gtattatctg ctactgaagt aatggaattg
    22861 attcctaatc gttatcctat ttgttatatt gattatgtag atactttaga accaggaaaa
    22921 aagatcgttg cgacaaaaaa tgtaacaatc aatgaagatt ttttccaagg tcactttcct
    22981 gacaatccag tgatgccggg tgcgttgatt ctagaaacac tggcacaagc aggttcgata
    23041 cttatcctaa aatcagaaga gtttctagga aaaagagctt atatcggtgg gatcaataag
    23101 gccaaattcc gtcaaaaagt tgttccagga gatgtgctga aatgtcattt tgaaatcacc
    23161 aaaatgaagg gatcagtcgg tacagcgtta tctgcagcat atgtagatga taaaaaagtc
    23221 tgtgagtgcg agttcacgtt catcgtcaac caaaatgctt gactataaaa cctgatatag
    23281 caaaacaaaa ggaagcttac ttgcttttta tgtaaaaaga agacgcctga atcctagcat
    23341 acgttgtcat ctatgcagga ttcagacgtt atttttttat ttttgttttg cttcttgcag
    23401 tatattgatt ttatcgattt ttacttcttc ttttggtttg tctgaactat ctgtttcgat
    23461 atttgcaata gcatccacga catccattcc ttcaatcact tggccgaaga cagtataatg
    23521 gccatctagt gaaggataac cgccgttttt ataagcgtcg atgattgctt gaggatagtc
    23581 gtcttttaaa agaccatcat gcatatcatc tgtattttga acgataaaga actggctgcc
    23641 gttactgtta ggatcttgtg agcgagccat agataacgca cctcttatat tataaaggtg
    23701 gttgttgatc tctgtttcaa atccttctcc ccaaatgctt tcccctccag tgccatcgcc
    23761 tttaggatcg ccaccttgga tcatgaaatc tttgatgacg cgatggaaag tcagtccgtt
    23821 gtagtaaccg tcttttgcat gtgtcataaa gttttcaacc gcttttggtg cgtgttcggg
    23881 aaataatttg attttgattg tgcctttatt cgtcaccatt tcgaccagat cttcattttc
    23941 ggcgacatct ttcgtcaatt gtggtaattc caatgcgttc aaatcgacgc tactggcagt
    24001 cgtgctttcc gcagtagatg tactttttga actttctgta gacgaactag aagaagtcgt
    24061 gttgtttgca gaacaaccag cagcaaacag caatgctgct gctaaactaa tacttaaaat
    24121 aactttattt ttcttcataa ctgttccttt ccaatttact attttgttat ctttaatgat
    24181 aacaaaatag agggaaaata cctacttaaa ttattggaat ttatcattta ttttaaaaga
    24241 aaaagaaaag gtgtagacca tttactttgt gtttgctata atgtaagtgg atacaaaaac
    24301 gaaaagatgc aggaggaaat ggtaatatgg gaaaattagg aatctcgatt tatccagaac
    24361 ggtcaacatt tgaaaaggac aaagcgtact tggatttagc tcataagtat gggtataaac
    24421 gggtttttac cagtttgctg cagatcaatg atgataaaga aaaagttctt tcagaattta
    24481 aagaagtagt tgattacgcg aatagcctag gcatggaagt aatggttgat atcaatccag
    24541 cgttatttga acagttggag atttcttacg atgacttatc ttttttccat aagatgggtg
    24601 cctacggggt ccgcttagat atcggattca caggagcaga agaagcaaaa atgacgcgca
    24661 atccttttgg tatcaaaatc gagatcaata tgagttcggg cacgaattac gttgacaaca
    24721 tcatgagtta ttcgccaaac acggataatt tattaggttc tcataatttt tatcctcatc
    24781 gatactctgg tttaggatac gaacactttg ttttttgttc agaaaaattc agaaaatata
    24841 atttgaatac gatggcattc gtcaattcgc aatctgctga gtttggccct tggcctacgc
    24901 aagatggctt gtgcacgctg gaagaccacc gagatcttga aattgcgaca caagtgaagc
    24961 atttaatcct tacaggattg attgatgata tttctattgg aaatgcgtac gcaagtgaag
    25021 aagaattgaa agaaatggca gaagcattta acgcggatta tccaacattg aaagtagata
    25081 cggaagaagg tattacagaa aacgaacgaa tctgcttgtt tgataacctt catagttatc
    25141 gaggggatcg ctcagaatat atccttcgtt caacgatgac tcgtgtatac tacaaagata
    25201 aagatttccc gcctcataat acgcgagata tgcatcatgg ggatgtactg attgacaatg
    25261 aagggtacgg tcaatacaaa ggagaaacac aaatcgcctt gaaggatatg aaaaacgacg
    25321 gacgtgtcaa tgttgtagga agaatctccg atgacgaatt attcctcctt gactttttga
    25381 aaccatggtc aagctttaaa ttaattgaaa ataaaaaata gataattagt tattgcagaa
    25441 ataatccgct agaaatagcc agttatttct gcttttttat tttgtttttt tataggttat
    25501 gatttgtcaa attaagctag aataaaatgc ttcttgtaca tagctcaaaa atatttcgat
    25561 tggtgttctg taattcaatg attttcttgg aatatgatta cgttgattgc tgacactgga
    25621 aataaatgtc tgattcactt ttctaaaatc cattgatttc ggcagtccat tacgacgcag
    25681 aatcccgtta gaattctcgt ttaatgggcg ttgagaaggt gttccagggt ccgcaaaata
    25741 tatatcaata tcatgttggt tactaatggc tttccagtta gaaaattctt ttccacagtc
    25801 aaacgtaata gatttaaaga agtttttagg gaagcgagaa aaccattgat taagggcagt
    25861 ttcaatatct aatgccttac ggccgttggg tttaatcgtg ataatgactt tagataatct
    25921 ttcaactaaa gtaatgacgg cacttttatg atgaatgcca acgatcgtat caccttcaag
    25981 gtgaccaaac tcagaattga aatcaggata attatcaggt cgatcatgga ttgagcgctg
    26041 atactgttgt tttccccgtt tttcctgatg gccattgggt tttcttttac ctttcatcgg
    26101 tagtgtgtca atatcaaata ttcctttaga aaataaacga taaagtgttc tcataccgca
    26161 tgaaacagac ctttcttttc gcccgataat gacgtcaggc gtccaaccaa gagtgacttt
    26221 ctctttaatg taatctactt catgagcagg taattgaatg acttttctac cacatttttt
    26281 cttattttct ttatactggt gccaataatc aagagcagtc ttgccttgct tgaacttatt
    26341 gactacatta taaatagttt gtatagcacg tcccattcgg ttagcgattt caaccggctt
    26401 attatgttga agataatatg attctatcat tgtcagttcg tctatggtaa gatgtttgta
    26461 ggtcatttat ggttacactc ctttgttttc tttcgtcgga aatacaattt gagtgtacca
    26521 taaatgcctt tttatttttc taacttaatt ttacaattcg cgggttataa aaatgaacat
    26581 tgcatagtag agattctttg attatactaa ttcaaggtta tatgaaaaaa gagaaatgag
    26641 atggatgatt tatgaaactt tggatgaaaa tccttttagg cgtcgcttta gctctgattg
    26701 ttgcgttagt gtttgcaggg aattattttt ataattatgc tgttgtgcct tctgagaaag
    26761 actttttaga cgaagataca cctggtacaa cacagacatc caaagaaacg gatgcacata
    26821 aatggtttag ttcaaaagaa aaccgaagcg attggaccat cacttcaaaa gacggtctga
    26881 aattatcagc gatttatctt cctgctgaaa cgaaaagtga aaaaacagct cttgtggctc
    26941 atggctatat gggaaacgct gaaacgatga ccaattacgc aaagatgttt catgatatgg
    27001 gttacaatgt ccttgttcct gatgcaagag gacatggaaa aagcgaaggg gattacattg
    27061 gatttggatg gccagagcgt aaagactatg tacagtggat aaacaaagtg ctagaaaaca
    27121 atggaaaatc gcaagaaatc gttctatacg gtgtcagcat gggagcagca acagttatga
    27181 tgactagcgg agaaaaacta ccagataatg tgaaagcaat cattgaagat tgcggttatt
    27241 cttccgttca tgatgagctt gcttatcagc tagacgatat gtttagtctt ccagcatttc
    27301 cgcttatgca agtaacgagt ctagtgacaa aagtccgtgc tggctacttt ttcggtgaag
    27361 caaatgcagt ggaacaattg aagaaaaacc agcgcccaat gctctttata catggagacg
    27421 aagatacatt tgtaccattt gaaatgttag ataaggtcta tcgtgcaacg aagggaccaa
    27481 aagaaaagta tgttgtaaaa ggagccgagc atgcggaagc atacaagaca gatcctgaaa
    27541 aatatcagca agtagtccaa caatttttaa gccaatatgt tcattgaacg agtttaaaag
    27601 taggggatga aaagaaagaa gcctttgact ggatccagtc aaaggcttct tttatcgatt
    27661 gatctatcaa attacaagtt agcgaatttt tctagtaagc gaatcatttg gcaagtaaat
    27721 ccatactcat tgtcatacca tgcaacagtt ttaacaagtt ggaaatcacc agcagttgtt
    27781 acttctgttt gcgttgggtc aaagatcgaa ccttgagttg ttccaatgac atcgctagaa
    27841 acgatttgac ggtcatcata cccgaatgaa ggattgtcaa ctgtgtgttt tttgattgct
    27901 tcgtttactt gatcagcagt tacttttgtt ttcaatactg aaaccaattc agttaatgaa
    27961 ccatcaacga ctggaacacg ttgtgcgtgt ccttgtaatt ttccttgtag ctcagggata
    28021 actaagccga ttgctttcgc agcaccagta gaatgtggga tagtattgtc agcagctgaa
    28081 cgagcagcac gcaaattacc gccttttaca ggtccatcta agagcatttg tgtggaagtg
    28141 taggcatgaa ctgttgtcat tgtaccaact tcgatcccaa attcattatt taagaaataa
    28201 gccataggtg ctaaacagtt tgttgtacat gaacctgctg agatgatttt gtcgttttca
    28261 tctaatgtat catcattgac gttatagacg atggttttca tagcaccagc tggggcagaa
    28321 ataactactc gtttgacacc agcatctaaa tgcgcttttg ctttttcttc agatgtataa
    28381 aaaccagtac attctagtac gatatctaca ccgttttctt ttgcccaagg gattttgctt
    28441 gcgtcgggtt ctgcataaac acgagttgct tcgccatcca ctacgattga gtcttctgtt
    28501 gcagtaactg tgccaggata agtaccatga gtagagtcaa attgcaataa ttgtgctaac
    28561 atagttggac ttgttaagtc gttgattgct actacttcaa tatcatctga gacttctttg
    28621 atgcggcgga aagccagacg gccaatacgc ccaaatccgt taatacctac ttttactgtc
    28681 atatcaaaag ttcctccttt attaggggtg ctgtgaaacg gcatcctcaa ttaatacaat
    28741 taatacactg ttattctgct acgcaaagaa tattttgtca aaaaattata cttggtagca
    28801 gaaaaaaggt tggaaacctt accaaatgat aaaaaaagaa gaaggtaaac aatctttttg
    28861 ctgaaatgat agacaaaatc agatttcacc gataaaataa gtaagacttt tgtttattgt
    28921 ggaaattcag gcagtcaaat aaataaatga ataataggag gacttaatta tgtccatgtt
    28981 tttagatcaa gtaacgatcg atgtgaaagc tggcaagggc ggagacggaa tggttgcatt
    29041 tcgtcgcgaa aaatacgttc ctgacggcgg accggctggc ggagacggtg gtcgtggcgg
    29101 cgatgtcatc ttgatcgtcg atgaagggtt acgtacattg atggattttc ggttcaatcg
    29161 ccatttcaaa gcgcagccag gtgaaaatgg gatgagtaaa gggatgcacg gtcgtggttc
    29221 agaagatacc tatgttaaag taccacaagg aaccactgtc cgagatgcgg agactggtgc
    29281 tcttttagga gatttaattg aaaatggtca aacactagtt gtggcaaaag gtggacgcgg
    29341 tggacgaggg aatatccgtt ttgcctctcc tcggaatcct gcaccggaaa ttgctgaaaa
    29401 cggtgaacca ggacaagagc gaaaaatcga actagaattg aaagtattgg cagacgttgg
    29461 acttgtaggt tttccatctg tcgggaaatc gactttacta tccgttattt catctgctcg
    29521 tccaaaaatc ggcgcttatc atttcacgac gctcgtacct aatctaggaa tggttactac
    29581 aagtgatggc agaagttttg ccgcagcaga cttgccaggc ttgattgaag gagcttcgca
    29641 aggtgttggg ctgggtacac aattccttcg tcatatcgaa agaacacgag taattttaca
    29701 tgtgatcgat atgagtggga tggaaggacg agatccttat gaagactatc ttgccatcaa
    29761 caaagaatta tctacttata acttacgctt gctagagcgt ccgcaaatca tcgtggcaaa
    29821 caaaatggat atgccggatg caccggaaaa tctagtgaaa ttcaaagaac agctgaacaa
    29881 ggaaaaagaa gatgaatttg ctgatgacat cccagtattt ccaatttctg gagtgactcg
    29941 acaaggattg gacgcgttat tgaatgctac agcagatttg ttagaagtaa cgccagaatt
    30001 cccattgtac gaagaagaac tggaagaaga aactgtacac tatggattca atccagaagg
    30061 tcctgagttc caaatcgatc gtgattcaga tgcaacgtgg atcttatctg gtgagaagat
    30121 cgaaaaatta ttccaaatga cgaatttcga tcatgatgaa acagtgatgc gttttgctcg
    30181 tcaattacgt ggtatgggtg tggatgaagc tcttcgggca cgcggtgcga aagatggcga
    30241 ccttgtacgt atcggtgagt ttgaatttga atttgtcgaa taggcaagca aataaaaaca
    30301 aataccaaaa aatatagatt cagatttaag ggaggttgtg acagaagtga tcagcttcaa
    30361 gaacgccgtt tattcggaaa aaggagctgt gatacgactt ttgtcacagc cctattttat
    30421 tggaaaaaaa gagtgctttt cttttcttat ttttcaatta aaggtaaaat aaataacaag
    30481 aaggtatgaa aggggcgttt ttaatgagca aacgagtaat cattttaaat tttcaagtag
    30541 aaagccaggc atatcaggca ttttcagaaa tcaaaaagtt acatgctgtt cgttcgatta
    30601 aaggagaaca gatggcagtt gtcagccatg atcctaatgg gacacatcaa ttcaaaatag
    30661 aagattttat cgactttacc ggtaataaca aatcttctac gggtggatta atcggaatgt
    30721 tggttggtgt attaggtggt ccgctaggga ttttattagg atggtttgcc ggaggaatga
    30781 tcggtgcgac acaagatgcc aaagaaataa aagatgccca gaccgtgttc gactttgtag
    30841 gaaataaaat cggtgaagga gctacaggac tgatcttgat tgctgatgaa gaggacaatc
    30901 gcccactgaa tcaattggta atgatggaat taggcggaga aattactcgc ttggattatg
    30961 aagatgtcga aaaagatgtg catgatgcga aagaagctga aaatcatgcg aaagaagctg
    31021 cacaaaagac ttggaaggac aaacatccgc aaacttccaa cgacaatgaa agttctggat
    31081 cttcaaatta ataaaaaaca gcagaggatc tcaaacgggt tcgctgctgt ttttatagta
    31141 attttcaaag aaaacgctat cgctttagaa cgttattttt aatttgaacc cattctcgac
    31201 ttacggttga ttatcccttc tttttaccct aaaattaaag aggaacttaa agaaagtcag
    31261 gtagattatg gaattacaat ttttaggaac aggtgcaggt gttcctgcaa aacatagaaa
    31321 tgtgacaagt atagcattga aacttttaga cgagcgcaat gaagtttggt tgttcgactg
    31381 tggcgaaggc acgcaaatgc agattttgcg aacgactatt cgtccgagaa aaatcggtaa
    31441 aattttcatt acacatttac acggcgatca tatttttgga cttcccgggc tcatcagcag
    31501 ccggtcattt caaggtgggg atacgccatt agagatttac gggccaaaag ggatagagga
    31561 atacataaaa gtctctttag gtatttcgca aacgcgttta agttatccgc tgaaattcat
    31621 tgagctaaat gaaacagccc cgatttttac tgatcagcaa ttttctgttt atacaaaaaa
    31681 actaaatcat gggatcgaca gttttggtta tcgtgtggtt gaacatgatc ataaaggaga
    31741 attgcaggtg gatcgtctaa aagaattagg tattccagct ggaccactgt acggcaaact
    31801 gaagcaaggt gaaacgatcc aactagaaga tgggcggacg atcaatggaa aagatttcgt
    31861 aggtccagat aaaaaaggac gaatcgtgac tattttaggc gatacgagaa aaacacataa
    31921 ttccgttgtg cttgcggaaa atagcgatat tctggtacat gaaagcacat ttaataaaga
    31981 tgaagcaaga atggcgcaca actatttcca ttccacgact catcaagctg ctgaagttgc
    32041 aaaagaagcg caggcaaaac ggctgctact tactcatatc agtgcgagat atttaggaaa
    32101 agcagcttta gagcttgaaa aagaagcaca agaagtgttt gaaaacacga cgattatgaa
    32161 agattttgat agcattgaga ttccatttcg cgaggaggaa gaggcatgaa tttagaagat
    32221 aaagttgttt tagtaacagg tagttcaggc ggtctgggcg cacaaatctg ttatgaagca
    32281 gcaaagcaag gagcaatcgt tataagttgc gcacgaagaa tggcatttgt agaaggtgtc
    32341 agagacgagt gcagacgctt gagcggcaaa gaggcttatg ctttcaaggt agacgtcagc
    32401 aatcctgaaa gcgtggacga attatatgaa aaggtgatgg aagaagtcgg gcgcgtcgat
    32461 attctggtaa acaatgcggg ttttgggatt ttcgaagact tccttacatt cgaccttgga
    32521 aaagcatacg atatgtttga agtcaacatc ttaggaatga tggttttgac acaaaaattt
    32581 gcgatcgata tggcagaaag aagacaaggt catatcatta atatcgcctc tatggctgga
    32641 aaaatggcaa ctgcaaaatc aacggtttat tctgccacga aatttgcagt ccttggcttc
    32701 tccaacgctt tgcgattaga attgaaacca cttggcgtag ctgttacgac agtgaatcca
    32761 ggtccaatcg agacaaattt cttcgataag gctgatccaa gcggaagcta tttggaaaaa
    32821 gtcggacaaa tcgttttaga acctgccaag ttggcaaaaa tgatcgtccg caatatgaga
    32881 catccaaaac gggaaatcaa tcaaccgttt attttagaag ttgcatcaaa attttatacg
    32941 ctgtttccaa cgattggtga ttatttagcc agcggaattt ttaacaaaaa gtaggtgaag
    33001 gaattgaaaa agcaaagcaa tgtgattctt ggatttgttt tagttttgat tattgttctt
    33061 tttgctgtat tgaacaataa aaatgttcct gtaagttttg gcttcacttc cttttctgcg
    33121 ccattgatct tagtgatcat cggttctgcg ctgattggtg cactgattgt atttttgact
    33181 gcttcggcaa cactatggca gcaaaaaaag caaatcaaac agctgactca ggaaatgtca
    33241 gaatatcaaa cagaacttca aaagaaaatt gaagaagcaa aagaagaaca gcaacgcgag
    33301 tttagaaacg aacgtgcgga gttggaagct gcttatcagg cggagttaca agcgaaaacc
    33361 gcacaaatca gtcaattgaa agcgccggaa gaacaaaaaa ctcaatcgcc caaacaaagc
    33421 ttcaattatt ttgactaata gggatatttc ataaatggtc gtacaaaaga tcaggtcctg
    33481 ctgaacatct gtctagcgga tctgatctgt ttttattctt tctttctgct tttttttgct
    33541 gatttccttt gctataataa ctaagttagg agtgagaaag tgagacaagc aaattttgat
    33601 tggaagttaa gcgagacaga gccttctgaa tcctttctcc aaatgatccg tgaactgaaa
    33661 ttatctccat ttgtcggtaa gttactgtgg caaagaggtt accatgagga aaaggatatc
    33721 aaccgatttt tacatccaaa ggaacaagaa ttacatgatc cgtatctgat acacgatatg
    33781 gataaagcaa tcagccgaat ccaagaagct gtaatcagtg gagaaaaaat cctcgtttac
    33841 ggtgattatg acgcagatgg gatcaccagt gcaacggtaa tgaaagaaac actggaatta
    33901 cttggtgcag aagtagaagt attcctgcca aatcgatttg agcatgggta tggaccaaac
    33961 caatcggtat accaagaaaa gatcgaagaa ggaatccagt tgatcatcac tgttgataac
    34021 ggggtagcag gaaatgatgc ggtagcatac gctcagcaag ctggtgtaga cgtgatcatt
    34081 acagatcatc acgaactacc tgagaattta ccagacgctt atgcgattgt tcaccctcgt
    34141 catccagcgg ggcactatcc atttccagat ttggcaggag tcggagtagc attcaaagta
    34201 gcttctgcat tattggaaga accgcctgca gaatttttgg atttagtagc gataggaacg
    34261 atcgccgatt tagtttcatt gactgatgag aatcgaatcc ttgtttcatt aggaatcgat
    34321 gccattcatc attcagaacg gataggactt caggcgctat ttgaagaaag cggcgtaaaa
    34381 atgagggacg cagacgaaac aacgatcggt ttttcgattg ctcccagact aaatgcaatc
    34441 ggtcggatgg gcgatccacg tcctgccgtc tcgttacttg cgacgtttga tgaagaggaa
    34501 gcatccgttc aagcaaaaaa attgaatgag atcaatgaag aaagaaaagc aatcgtagag
    34561 cagataacac aagaagcatt agcgatggtc aataaggaga atcatatcca tctattggta
    34621 aaccgaggat ggcatgaagg cgttttgggc attgttgctg ggaaaataat gaatgaaacg
    34681 ggtaaaccaa cgcttgtcct tactttaaaa gaagatggat cagctaaagg atctggacgt
    34741 agtattgaag ccttgaatct ttttgaaatg cttgaccaga tgcgagattt atttacttac
    34801 ttcggaggac atcatgcagc agtcggttta acgatgccaa gtgaaaatgt cactatacta
    34861 caggagaaga tgaaccaata tatagtcgat catcaaattg acttgatgag agggcctgag
    34921 ttgagaattg atgaagtatt gctgccaaat gaagtaacag tagagcgaat tgatgagctg
    34981 aaactattgg cgccatttgg tacggataat cctttgcctc agtttttatt tcgacaagtc
    35041 caagcagaaa atatcaaaag aatcggagca aaccaacagc atctgaaatt tgtcctttcc
    35101 gatgcatcga gtcagcttga tgcggtcgct tttggttttg gtactcaaga agaagaactg
    35161 ctgaataatc aggtagatgt ggtaggaaag ctttcgatca atgaatggaa cgggcgtaag
    35221 aaaccgcagc tgatggtatc tgatttttct gtgggtggtc tgcaagtatt tgattggcgg
    35281 gcaaaacgtt tccgtgaaca gagccgaacg tcggaaggta gcctttattt agcatttgat
    35341 tcttcgacgt tgaaattcgt gcctgagatt atccaggaag ctgcgattgt tttcgaagga
    35401 ctagattctg taaaggcaat aattgaaaaa agaaaaccaa actctctagt tgttatggat
    35461 tgtccagatg atttagcgga tctgaaagaa gtttttcggt cttattcatt caatcgtgtt
    35521 tatctgatgg ggatttcacc tgatgaggcc tatttgaatg gggtaggcac aagagaacag
    35581 tacgcaaaat tgtttaaatt gatccattct caagaacgaa ttgatatacg tcataaaata
    35641 aaagcaatag ctcaatattt gcgtatccct gaaaaattat taatttttat gatacaggtg
    35701 ttttttgaat tgaaatttgt tacaatagag aacggtgttt tacaaaaagt cgctagccca
    35761 gaaagtcatc ctttgactga gagcagatta taccagcgac ggctcaataa aatcaaagtt
    35821 gaggaatttt tgcttttgag cgatattcct accataaaga aatggttaac aacctaggag
    35881 gaaacaaaga gtggatttaa aagattatat tgccagtatt cccgactatc cgtcaaaagg
    35941 aattgtgttt cgagatattt ccccactgat ggcagatggt gacgcttatc gggaagctac
    36001 taaacaaatc gtaaattatg caaaagaaaa acgaattgat atggtagttg ggccagaagc
    36061 ccgtggattc atcgtaggat gtcctgttgc ttatgagtta ggagtcggtt ttgccccagt
    36121 ccgcaaaaaa gggaagttac ctcgagaaac catcgaagtg acttatggat tagaatatgg
    36181 aacagataca ttgacattgc ataaggatgc cattaaacca ggtcaacgtg tgctgatttg
    36241 tgacgatttg ctagctactg gaggaacaat caaagcgacg atcgaactga tcgaacaatt
    36301 aggcggtgta gtcgtaggct gcgcattttt gatcgaattg atggatctcc atggacgcga
    36361 caaaatcaaa ggatatgaca tcattacatt aatggaatat taagcagaaa aaagaagcta
    36421 ttcttgatgt tattcttaaa gaagcgattt taaagcgacc ct
//
LOCUS       DAZBTF010000021        35882 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000021 DAZBTF010000000
VERSION     DAZBTF010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.021"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1152
                     /locus_tag="ACHGNS_001128"
     CDS             <1..1152
                     /locus_tag="ACHGNS_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HFN6937089.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="ACHGNS_001129"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="ACHGNS_001129"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFN6937090.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="ACHGNS_001130"
     CDS             2728..3003
                     /locus_tag="ACHGNS_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFN6937091.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="ACHGNS_001131"
     CDS             3009..3776
                     /locus_tag="ACHGNS_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFN6937092.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="ACHGNS_001132"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="ACHGNS_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFN6937093.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="ACHGNS_001133"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="ACHGNS_001133"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFN6937094.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="ACHGNS_001134"
     CDS             7920..8222
                     /locus_tag="ACHGNS_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFN6937095.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="ACHGNS_001135"
     CDS             8225..9388
                     /locus_tag="ACHGNS_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFN6937096.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="ACHGNS_001136"
     CDS             9414..12842
                     /locus_tag="ACHGNS_001136"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFN6937097.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="ACHGNS_001137"
     CDS             12863..13987
                     /locus_tag="ACHGNS_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFN6937098.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13968..14414
                     /locus_tag="ACHGNS_001138"
     CDS             13968..14414
                     /locus_tag="ACHGNS_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFN6937099.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="ACHGNS_001139"
     CDS             14398..14736
                     /locus_tag="ACHGNS_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFN6937100.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="ACHGNS_001140"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="ACHGNS_001140"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFN6937101.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="ACHGNS_001141"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="ACHGNS_001141"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFN6937102.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="ACHGNS_001142"
     CDS             15893..16936
                     /locus_tag="ACHGNS_001142"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFN6937103.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="ACHGNS_001143"
     CDS             17095..17823
                     /locus_tag="ACHGNS_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFN6937104.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="ACHGNS_001144"
     CDS             17939..18184
                     /locus_tag="ACHGNS_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFN6937105.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="ACHGNS_001145"
     CDS             18181..18399
                     /locus_tag="ACHGNS_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFN6937106.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="ACHGNS_001146"
     CDS             18535..19074
                     /locus_tag="ACHGNS_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFN6937107.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="ACHGNS_001147"
     CDS             19067..21340
                     /locus_tag="ACHGNS_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFN6937108.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="ACHGNS_001148"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="ACHGNS_001148"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFN6937109.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /gene="rpsT"
                     /locus_tag="ACHGNS_001149"
     CDS             complement(22513..22767)
                     /gene="rpsT"
                     /locus_tag="ACHGNS_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFN6937110.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="ACHGNS_001150"
     CDS             complement(23167..23436)
                     /locus_tag="ACHGNS_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937111.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23698..23826)
                     /locus_tag="ACHGNS_001151"
     CDS             complement(23698..23826)
                     /locus_tag="ACHGNS_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937112.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(23906..24361)
                     /locus_tag="ACHGNS_001152"
     CDS             complement(23906..24361)
                     /locus_tag="ACHGNS_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFN6937113.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..26427)
                     /locus_tag="ACHGNS_001153"
     CDS             complement(24499..26427)
                     /locus_tag="ACHGNS_001153"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFN6937114.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26624..27457)
                     /locus_tag="ACHGNS_001154"
     CDS             complement(26624..27457)
                     /locus_tag="ACHGNS_001154"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFN6937115.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27649..28353)
                     /locus_tag="ACHGNS_001155"
     CDS             complement(27649..28353)
                     /locus_tag="ACHGNS_001155"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFN6937116.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28376..29002)
                     /locus_tag="ACHGNS_001156"
     CDS             complement(28376..29002)
                     /locus_tag="ACHGNS_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFN6937117.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29328..31703
                     /locus_tag="ACHGNS_001157"
     CDS             29328..31703
                     /locus_tag="ACHGNS_001157"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFN6937118.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(31727..32218)
                     /locus_tag="ACHGNS_001158"
     CDS             complement(31727..32218)
                     /locus_tag="ACHGNS_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFN6937119.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32405..33325
                     /locus_tag="ACHGNS_001159"
     CDS             32405..33325
                     /locus_tag="ACHGNS_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN6937120.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33479..33823
                     /locus_tag="ACHGNS_001160"
     CDS             33479..33823
                     /locus_tag="ACHGNS_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFN6937121.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(34013..34513)
                     /locus_tag="ACHGNS_001161"
     CDS             complement(34013..34513)
                     /locus_tag="ACHGNS_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6937122.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            35041..35124
                     /locus_tag="ACHGNS_001162"
     tRNA            35041..35124
                     /locus_tag="ACHGNS_001162"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35075..35077,aa:Leu,seq:cag)
     gene            complement(35448..>35882)
                     /locus_tag="ACHGNS_001163"
     CDS             complement(35448..>35882)
                     /locus_tag="ACHGNS_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFN6937123.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        11868 a         6262 c         7405 g        10347 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttgtttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgctg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca
    24961 gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg
    25021 gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg
    25081 ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga
    25141 aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt
    25201 gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa
    25261 ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc
    25321 aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg
    25381 ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt
    25441 tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc
    25501 agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga
    25561 aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac
    25621 taataatacc attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg
    25681 gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg
    25741 ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat
    25801 cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg
    25861 attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc
    25921 gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac
    25981 agctgctcct ttagcgacaa ataccaagtt atccaccttt agttgagaaa catctacttc
    26041 ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat tcatcaaagc
    26101 agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc ttaaacaaaa
    26161 gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac caatagcagc
    26221 taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt tcaagccttc
    26281 cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg gggtgatggt
    26341 aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc cgattgttgc
    26401 tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa acaaggatag
    26461 cataataaaa atgtaattga taataataac agctaaatga gaacgataat ggttatcatt
    26521 tggtgattaa gcttgatgtt gtttttatta tttataatga ttattattaa agaaaataaa
    26581 aaaccagaac atttctcaaa taagagaagt gttctggttt tttctacatt gcaataaacc
    26641 aataaccaat caaaatgata ccaaggataa tacgatacca gccaaaagcg gtaaagtcgt
    26701 ttttcttcaa atagcttagt aagaatttga tagctaagac ggaaaccaca aaagatacga
    26761 atgttcctac aagaagaaca acagtttctt ccattttgaa tgaattccct tcagctaaat
    26821 atttgacgat cttcaagccg cttgcgccaa acatgacagg aatccctaag aaaaatgaaa
    26881 actctgtagc gacaaaacgc gaagcaccaa tcaagataga tcctaggatc gttgcgccag
    26941 aacgggatgt tccgggaaca agtgccaaga cttggaataa cccaacaaga agagctgctt
    27001 ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt gtttttgttg cgtttttcga
    27061 caataataaa ggcaataccg taaacgatca aaacaatggc aactggtaag aacttgtgga
    27121 aatttgcttc taaccaatca tccaaaggca aaccaatgat gatggctggt atgcaagcaa
    27181 cgatcacttt aaaccaaagt gtccacgtat cttttttttc taccgttgat ttacttggag
    27241 aaaatgggtt taacttgtga aaataaagaa cgacgacagc caagatcgcc cctaattgga
    27301 taacgacatt gaacatcgac ataaattgtg aactggcatc cagtttgatg aattcatctg
    27361 ctaggatcaa atgaccagta ctactgatgg gcagccattc cgttattcct tcaataatac
    27421 cgagaatcac tgctttgata atatttgcaa aaaacataaa ttccttcctt cccaattaag
    27481 atacatctta tagtataccg ttattttttg agaaacccaa tctgtttttt aatattaaga
    27541 aaaacttgag aaataaagca gataagtaca cgaagagaaa aaagaagaca aagccctcgt
    27601 tcagactttg ccttttatga ttctctggta aaaattaaaa tatccccatt atagaaacga
    27661 gtcgcgacta tgtgttttcc cgttatcgat ataagacgta tcattgatgc cgtggataga
    27721 aaatcgaccg tttgcataag tgacctttgt tacgctgcca tttaaaagcc cagcacgaac
    27781 aggctgttta ggatcgatca gattaagcaa aaaggcgatc gtcaaaccat gagatacgac
    27841 taaaacattt ccaccaccat ttttttcgca gtgatgggcg atatcttcaa atcctgtcca
    27901 tacacggtct cgcaatgttt cataagtttc cgcccaccca gcggtatcta agttgtatac
    27961 agcattagca atttgttcaa aagaaacttc tgtggtaaac atttcatcat atgttttaaa
    28021 attcaataca cgagggatca caccccacat ttccatgtca tatccacctt caagagaccc
    28081 gaaacaccat tctctaatcc ttttttcaat aaaataagga atttcttttc ccttagaatg
    28141 ttctcctaga ataatacggg cagtttcgat tgcacgacca ctgtcactag aataagcaaa
    28201 tgaaaagtcg acatttttta acccgcgacc taaataatga atgccttctt gtccttgctt
    28261 tgtgaggggt gtatcacacc agccttgtac tcgttcaatt gtattgaaca tcgtttttcc
    28321 gtgtcgtacg atataaagtg tagtttctgt cattttattc tcctcctaag tcgttttaac
    28381 gattgaaaaa aggatttgtc cttttttcct gttcaatcgt cgttgcatcg ccatgtcctg
    28441 gatagactgc aaattcatca ggtaatacaa acagctgggt ccgaatacta tgaagtaatt
    28501 gttctaaatt ccctgtatac agatcggttc taccgatact tcctctaaac agggcgtcgc
    28561 cagaaacgac aaaatcatca aaaataaagc tgacgcttcc tatagaatgc cctggggtag
    28621 ggacgacgcg aaaagacatg tttcctaaag tataagtttt catttcaaat tcatattctg
    28681 caggcgagac aataacatca gcaatatcat catgtctacc gagtccagaa agattcaaaa
    28741 tcggatcacc tagccatttt tgctctagtg ggctgacata aactgggatt tgataatacg
    28801 cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt atgggtaagc aaaatcgcta
    28861 ccggctgttg gtttgttttt ttgatttctt gtttgattcg ttccgcatct tctccagggt
    28921 caatgatcaa aagtgcttct tcgttgtaga ccaaataaca gttttcttca atcattcctg
    28981 tttttaatct ttcgatatgc atgtgcattc ctccttgata gccaaaactt ctttcatcca
    29041 ttagtataac gcattcattg ctaggaaaca aaaacaggta cttatttctc tttacttatc
    29101 tgaattatca gactatatga aaagtatttg gatattctaa agagaaggaa agtaagtttt
    29161 catagcattt taatatgtcc ttgttacatt agattcatcc aaaaatccaa attctatatc
    29221 ctatatccct gcctaatcag cagggtgttt ttttatgttt ggaaagagag ctctttttgc
    29281 taaactaggt tttttcaggt aatcttagag agaaagaggt ggtggaaatg atcaaacagt
    29341 caaaaatatc tgtgcggcac ttagtagaat ttattttgcg acgtggaagt atcgacaatc
    29401 gtaaaaaaag taaccatact gcattagaag gagcgagaat ccaccgcaag ctccaaaaag
    29461 atgcgggaga aaactatgaa aaagaagtct ttttaaaaac aacagttcaa ttagatgaat
    29521 attacctgat cgttgaaggc cgtgcagacg gtattttcaa aagagacggt atttattata
    29581 tagatgaaat caaaacctcc gaaccacgat ttgaagattt agaaccagaa caagtggaac
    29641 ttttctttca tcaagcaagg gtgtatgcct atatctatag tcaagaacat gatttacaag
    29701 aaataaatct gcaattgact tacttccaga cggcagaaga agtgatcacg agaaaggttg
    29761 aacatcagac aagagagcag ttagaaactt tttttaaaaa gctgactgaa gattataaag
    29821 aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa tgcctctttg atggctttga
    29881 aattccccca tgatgagtat aggaaaggtc agcgtgaact agcggtagct gcttataaaa
    29941 cgatccgtac gaaacagaaa ttgtttgtag aagcacctac cggaacagga aaaacaatct
    30001 ctacgttatt ccccgctttg aaagctgtag gggaagaaga gggagaaaag atcttttatc
    30061 taacggctaa aacaataact agacaagtag ctgaagatgc tatgacagca ttaaaagata
    30121 caggagctga agtaaaaagt gtcactttga cggcgaaaga caagatctgc tttttagatg
    30181 agacgacctg taatccagat cagtgcccat atgccaatgg ttattacaat cggattaatg
    30241 aaggtttatg ggatcttttg aatcatgaga atcagatcac tcgtgaagtc atcgaaactt
    30301 atgcaagaaa gcatacactt tgtcctttcg aactatcatt agatgtcagt atctggtgtg
    30361 acgtaatcat tggggattac aattatttat ttgatccaac tgtttattta agacgttttt
    30421 ttgaagatga aaaaaacgag gactatctgt ttttgattga cgaagcacat aatttggtga
    30481 atcgttcccg agagatgtat tcggcagaac tatcttatga aaaaacgaaa cgaagtaagg
    30541 aagctatacc aaaggaattc aaaaaacttc atcgccgttt caacaaacta ttaaaagaat
    30601 ttgattcgat ccgagaaata gcaaaagaag atcattggga ttatcatcat caaaaagcac
    30661 cggctgaatc tttagtaaag gcaggttatc aattaagcga aaaaattaaa gaatggctgg
    30721 cagaatttcc tgagcaccca tcacaagaac aattgcttcc ttattatttt gatttgcttc
    30781 attttttgaa ggtcagtgaa ttttatgatg atcactacga aacaacagtc gaaaaaacgt
    30841 atcgcgatct gatagtaaaa gagttttgta ttgatccaag cttgtttctt gagcagtcgt
    30901 tggataaagg aaaaagcagt attctttttt ctgctagttt ttcaccactc tcttattatc
    30961 aggaaacact ggggggacaa aaaagtctgg cttacaaact gccgagtcct ttcccagaag
    31021 gaaaccagca agttttcatc gcaaattacc tagagacgac ttatcgaaaa agggaacaca
    31081 gtctggcaaa attagtagag acgattcatg cttttgctga tggaaaggtc gggaattatt
    31141 tcgttttctt tccttcttat caatatttag atcttgcggt ggaagcattc agacagcaat
    31201 atcccgacag caatgtgcta atccaagaaa cggacatgaa tgaggaagaa cgcgaacgat
    31261 ttttaacaaa atttcaaaca aatcccgctg aaacattgat tggattttgc gtacttggcg
    31321 ggattttttc agaaggaatc gatttgagag gggaccgatt gatcggcagt atgatcgtag
    31381 gtgtaggttt gccgcagatg aatcatgagc aggaactgat caaatcatat tatgatgaga
    31441 aggaacacct tggatttgct tacgcttatc agcttcctgg aatgaacaaa gtcttgcaag
    31501 ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat cgttgtattg gcagatcaac
    31561 gattttctag tttttcttat cgacgtttat tcccacaaca ttggcaaaca gcaagagaag
    31621 tacgaagtgt tggggaattg gaagaaggaa tacagcgttt ttggttatca aaagaagcag
    31681 taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt ttttgattaa cgatagttag
    31741 tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa agcaatcaca gcttgtagat
    31801 catcacggtt cttacctgtc actctaatct gatcttcttg gatttgtgct tttactttca
    31861 gtttttcatt tttgatagct gtagtgattt tcttcgcgtt ttcacgatcg attccgttga
    31921 ccaaatcggc agtttgtcga gctttgccac ctagagcgtg ttctgattcg gaaaaatgga
    31981 tatttttgat tggcacattt cttttgatca gcttaccaaa taatacatct ttgatctgtt
    32041 cgattttgaa atcatcatcg ctcaatacaa ctagattgct gttctctaat ttgatttcta
    32101 ctgtagaatc cttgaagtca aagcgattgg acaattcttt tgttgtttgc tgaatcgcat
    32161 tttttacttc ttctgtattg acttctgata caatgtcgaa gctagcatct tttgccatag
    32221 gaaccctcct gatatacata taaatattta ccatacatct atcttatcat aggattaggg
    32281 cagattctta cctgtttgct aaaattcttc tcatttgagt tcgaagtatt ttattttaat
    32341 agataaactg ttattcttac atttggaata ccaaagtagg ggagaggtgc ttgtgagtaa
    32401 aattatgact gcgtatcata aaaatgaaag attaaagaaa atagtggtta ttttgataac
    32461 cggattgacc gctgcggtcg ctttaaatta ttttttgatt ccagccaatg tcttttctgc
    32521 agggatgaat gggatcgctc aaatcatttc ctcgctttta tatgaatggt ttcatatcaa
    32581 gatagacaca ggaacattta tttttctttt gaatatccct gtgtttgtat tagggtttct
    32641 caaagtagga aaacgagcaa cgatccttag ttttgtgaat gttgtatgtg tatcggtgat
    32701 gacgatgctg cttccagtcg gacagatcac tgataatatt ttgatgaatg ccctagtagg
    32761 cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa atgggcttta caactggtgg
    32821 gatggatatc atctctcttg tactttcaca aacaactgga aaaacagtcg gaaactacat
    32881 gctgattcta aatggtatca ttgtagtcat cgcaggcttc ttctttaact gggaaagtgc
    32941 cttgtatacg attatttcca tttatgcaat gactcaagta attgatgcca tccacacgag
    33001 tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca gaagaggtaa cagaagaact
    33061 agcaaaacgg atggtacgtg gaatgacatt actgccgtct gtcggtggct attcaaaaaa
    33121 acaagggaac atgatcatga tggtcatcac ccgctacgaa atgtatgatc ttgatcagat
    33181 cgttcatgaa atcgacgacg atgcatttat caatatttta ccaactcatt cagtatttgg
    33241 ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca actggcgtat tgccagaaat
    33301 aaaaaatcgt tcacaaaaaa aatgaggaat caggtttatt ttagaataaa tttaatctat
    33361 tacatctaat aagaacattt ttataaattt aaacgagaat cttacttcta aattctcatt
    33421 tacaagcagc taaaaaaaga gtatgataaa taaaaatcgt gttaaggagt gtcgtacgat
    33481 gagcgaagca aaccaagaac ggtctggaca agaaatagaa ctaatcaaag aacgtattct
    33541 tgctgccttg gaaatggtga ttgatcctga attaggaatc gacatcgtca atttaggctt
    33601 gatttatgat atagaattca atcctgaaaa tggagaaaca gtcattaaga tgactctaac
    33661 aactatgggc tgtccattag cagatatttt aacagaatct atccatgacg cattaaaaga
    33721 agtgccagaa gtaacgaaag cagaagtcaa actagtctgg tatccagcat ggacgacaga
    33781 taagatgagc cgttatgcac ggattgcgtt ggggatacga tagaaatctt ggctgactag
    33841 ctatgagaag caaataggaa aaacaaaaaa gccttttatc ctttctagaa gaaaagagat
    33901 aaaaggtttc tttgtttata aaattttgtc tgctatatta taagttaatt aaataaatgt
    33961 gttctattga taaaggacat tcaaatccct ttccttttgg aatccttctt ttttacatca
    34021 gtttgatttt cgtatagtat tctttctcat cttcacgata aatcgcagcc ccattttttt
    34081 gttgtgcttt aagactgcca agattttttt tatttacaga caaaagtgcc tcatcaatgc
    34141 cgattttttt tgcctcttgt agtactaatg ctaatccttg tgtggcatat ccttttttac
    34201 gataattagg ggagatgccg taaccaatat ggccagcgcc gtttcttaat gtttcgttca
    34261 gtttatggcg caaattaaaa attcctacat actcatgttc atcactttct aaaataaaga
    34321 cggttgcggg aacgtaactc tcaggaagat cgattcctaa agacgttttt tcgcattttt
    34381 ttacataatc taaaaattca gcaaatgtat agccataagc attgttgata aatccactct
    34441 catttggtcc aaagctagtt tgtagctgat aagctttctt taaatctgct tcccatagtt
    34501 tgatcagttt catcgtattc acctctttaa gtaaccttat tcatttgttt tctactatag
    34561 cactattgtt gtaaatagcc atgcctattt ttatttattg tgtcatatcg tattaagaat
    34621 catcgaacaa tacaaatatg aattacaata aaaaaattat ttgaagttcg ttcattcgtt
    34681 acttatgatc tctagaagaa agaaaacgtg aattacttca gcgtctagaa ttccagaagg
    34741 atagtaatca acagaagaaa taatcaatat ccaaatgaaa gaagatagct gaatcgagaa
    34801 aaagaggaaa aaagaaatga agaaaaaaat aaaatagtta ctaatcccag agtcgttcgt
    34861 ttaacattga aatcgatcat atggataaaa cagatttttt caccaaccct caatagatga
    34921 tggagttgct cggatgaatt atttgtgtag atggcataag ggagggcgta cagaaatgaa
    34981 aaagaacaaa aatatgttct tgacaaaaaa cacatgtaat cctaaactaa aagatgtgat
    35041 gcggctatgg cggaattggc agacgcgcta gcttcaggcg ctagtgaggg tgacctcgtg
    35101 gaagttcgag tcttcttagc cgcataattt gtttttttat aaagagaagg tacacctgga
    35161 gcgatgagaa agaaaataac atattatcta aaagaatata ggaaaaattt taaacaacgg
    35221 aaccagattc aaataaatca aaaaaaattt gtgtgaaggt gggacagttt aaaacctaat
    35281 agattgattc aaatagtaga tttatagatt ctgatacaag aaatactggc aaacgatagt
    35341 taaggaataa agactaaact taaactcaaa aagaggtcat gcgaaagaat aaatcgcatg
    35401 acctcttact atattttgaa tgtgtttaaa cgttcaaata atgaatctta gtaccaaccg
    35461 tttgcttccc agaatgcttt cgccgcttcc caagaaccat aacgtgaagt aacatattgt
    35521 tctgctactc tttcttggtt tgcagcagag tagtcaccat ttaagtaaga agaatctaat
    35581 tggtaacggc cgatgtaacg tccgttcgtt gctgtgtaag aaccgcttga ttctttttgt
    35641 gcgatccatt cttttgcaga gcttgtactt gctggagtag cagcagtttc tgttacagca
    35701 ggagcaggtt gtgtttcagc aacaggcgct gcttgtgttt ccgtttgaac cgtttcttgt
    35761 acaacaggtt gttcagcttg aacaggttca gcttggactg gtgcttcttg aacggctgct
    35821 tgaggttcag cagctgcttg tgcagagcct tctgttggga ttgttaattg ttgtcctgag
    35881 ta
//
LOCUS       DAZBTF010000022        34962 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000022 DAZBTF010000000
VERSION     DAZBTF010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.022"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="ACHGNS_001164"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="ACHGNS_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFN6937124.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="ACHGNS_001165"
     CDS             complement(2020..2922)
                     /locus_tag="ACHGNS_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN6937125.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="ACHGNS_001166"
     CDS             complement(2919..3611)
                     /locus_tag="ACHGNS_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFN6937126.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="ACHGNS_001167"
     CDS             complement(3654..4295)
                     /locus_tag="ACHGNS_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFN6937127.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4647)
                     /locus_tag="ACHGNS_001168"
     CDS             complement(4333..4647)
                     /locus_tag="ACHGNS_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937128.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4635..5264)
                     /gene="pyrE"
                     /locus_tag="ACHGNS_001169"
     CDS             complement(4635..5264)
                     /gene="pyrE"
                     /locus_tag="ACHGNS_001169"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFN6937129.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="ACHGNS_001170"
     CDS             complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="ACHGNS_001170"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFN6937130.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5984..6916)
                     /locus_tag="ACHGNS_001171"
     CDS             complement(5984..6916)
                     /locus_tag="ACHGNS_001171"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFN6937131.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="ACHGNS_001172"
     CDS             complement(6913..7689)
                     /locus_tag="ACHGNS_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFN6937132.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="ACHGNS_001173"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="ACHGNS_001173"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFN6937133.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /locus_tag="ACHGNS_001174"
     CDS             complement(10933..12009)
                     /locus_tag="ACHGNS_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFN6937134.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="ACHGNS_001175"
     CDS             complement(12014..13294)
                     /locus_tag="ACHGNS_001175"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFN6937135.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="ACHGNS_001176"
     CDS             complement(13313..14239)
                     /locus_tag="ACHGNS_001176"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFN6937136.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="ACHGNS_001177"
     CDS             complement(14252..15535)
                     /locus_tag="ACHGNS_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFN6937137.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="ACHGNS_001178"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="ACHGNS_001178"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFN6937138.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="ACHGNS_001179"
     CDS             complement(16323..17234)
                     /locus_tag="ACHGNS_001179"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFN6937139.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /gene="lspA"
                     /locus_tag="ACHGNS_001180"
     CDS             complement(17231..17695)
                     /gene="lspA"
                     /locus_tag="ACHGNS_001180"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFN6937140.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="ACHGNS_001181"
     CDS             complement(17700..18398)
                     /locus_tag="ACHGNS_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFN6937141.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="ACHGNS_001182"
     CDS             complement(18385..18771)
                     /locus_tag="ACHGNS_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937142.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="ACHGNS_001183"
     CDS             complement(18918..20588)
                     /locus_tag="ACHGNS_001183"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFN6937143.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="ACHGNS_001184"
     CDS             20771..21004
                     /locus_tag="ACHGNS_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN6937144.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="ACHGNS_001185"
     CDS             complement(21103..21534)
                     /locus_tag="ACHGNS_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFN6937145.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..22026
                     /locus_tag="ACHGNS_001186"
     CDS             21613..22026
                     /locus_tag="ACHGNS_001186"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFN6937146.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22064..23212)
                     /locus_tag="ACHGNS_001187"
     CDS             complement(22064..23212)
                     /locus_tag="ACHGNS_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFN6937147.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23334..23930)
                     /locus_tag="ACHGNS_001188"
     CDS             complement(23334..23930)
                     /locus_tag="ACHGNS_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFN6937148.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="ACHGNS_001189"
     CDS             complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="ACHGNS_001189"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFN6937149.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27160..27591
                     /locus_tag="ACHGNS_001190"
     CDS             27160..27591
                     /locus_tag="ACHGNS_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6937150.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27636..28985)
                     /locus_tag="ACHGNS_001191"
     CDS             complement(27636..28985)
                     /locus_tag="ACHGNS_001191"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFN6937151.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29055..30218)
                     /locus_tag="ACHGNS_001192"
     CDS             complement(29055..30218)
                     /locus_tag="ACHGNS_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFN6937152.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30347..30670
                     /locus_tag="ACHGNS_001193"
     CDS             30347..30670
                     /locus_tag="ACHGNS_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937153.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30853..32202)
                     /locus_tag="ACHGNS_001194"
     CDS             complement(30853..32202)
                     /locus_tag="ACHGNS_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFN6937154.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32271..33224)
                     /locus_tag="ACHGNS_001195"
     CDS             complement(32271..33224)
                     /locus_tag="ACHGNS_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFN6937155.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33326..34516)
                     /locus_tag="ACHGNS_001196"
     CDS             complement(33326..34516)
                     /locus_tag="ACHGNS_001196"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFN6937156.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            complement(34716..>34962)
                     /locus_tag="ACHGNS_001197"
     CDS             complement(34716..>34962)
                     /locus_tag="ACHGNS_001197"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937157.1"
                     /translation="IRKNRTVTRLLSQSFFRIQQVKLLRNIQRVKLKIADKRRWKFYV
                     FLLVYAINKLNIQNTIDFNERASINGCVIFICPGRVK"
BASE COUNT         9952 a         7641 c         6120 g        11249 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttaaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagcag ataatcttgc acgtcaggct ctccataaac aattaaaaga aaaaaagact
    22021 ctttaaatta aaaactttaa ggagtctttt tatagtttct catttaatat aaaagataat
    22081 tgtgatcaaa cgtaatttcc aattggtaat ctccgatttt ttcttctatt tgactacgta
    22141 agattctttc tagttctgta tcaacaaact ggaatttctc agggaccact acatcaaatt
    22201 gcagcgtttt tccttgatga attactctaa aatcatgaat attaagccct gtacctgttt
    22261 caagaagaat ttgctttaac tgccgcaaga tattatagta tttttcgtta tcaacaggca
    22321 ttggatccaa gtgacagaca agttccacat ccaaagactt tttaaagtct ttttctatca
    22381 gatcaataat cttatgagcc acattcaaat tcattgattc atctacttcg atgtggactg
    22441 atgcaaagcg tttattgggt ccatagttat gcaccaataa atcatgatag ccaagtatat
    22501 catcgcaagc atttaaatgc tcttccattt cttcaatctc tgctggcgtt gggcgacttc
    22561 ctaatagttc gtatacaaaa tttcgaagca tcgtcaatcc actataaagg atatataatg
    22621 ctaaagcaaa acctatatat ccatcaatac gccatccagt tacccattcg attgctgctg
    22681 aaagcaatac agccagtgta gtaaagacat cattataact atctgttgct gtagctctca
    22741 atgtttcaga atcaatggat tgcgcaataa cctgatacat tttgccttgt gcaaatttca
    22801 aaaagatgga aatgattagg acaaaaaaga cgatgggtgt taaagagaca ttttctggat
    22861 ggatgatttt ttcagcagaa gactctaaaa attgaaatcc tacataagta atgatgatcg
    22921 aaacaaataa cccactgata tattcgaagc gttcgtgtcc ataaggatgt tcctgatctg
    22981 ctggctttgc agcaatttta aatccgatca aagtcaatat ggatgaagca gtatctgata
    23041 gactgttcac tgcatcagcc ataatggaga cacttcctga aaaaagcccg atcattagct
    23101 tagagacaaa caagatcaga ttcgtaacca atcctaagat acccgctagt actccttttt
    23161 ttgtccgctg ttgcggtatt tgtttctttt tttcttcttg tttcttagcc aagtttttta
    23221 ccccttctag tttgcttgtt gctcaggtaa aaagatggag aggacgattg ttttcctctc
    23281 ctctcttcct ttttctatct atcgtgcgtc ctattactat taaaagatgg tcattattct
    23341 ggtttcaacg gtgttgctgc aaataacgct tttgctagta atggtgttat gattcccgca
    23401 cctattgctt ctggtatccc attgattgct gcaacccctg ctaaggtttt aaataaaaga
    23461 gcagagtcta caccataggc atctgccgtt gcacctgtat aaaataatcc catgaatcct
    23521 aacactaaga ctgtattgac caatgaaccc agtacccctg caaaggctga gctgatcgtt
    23581 attgatttat ttttttgata aatccagtgg aaaaccaagc ctgctactaa gccaacaagt
    23641 actctgggaa ctaccgagat aatcggattc gtgaacacta atgtatcaaa tggcgttgtt
    23701 ggcatggcat aggcacgaac aattgtagcg attccccaaa acaagccaat caccatgcca
    23761 tcttttgggc ctaatactac tgcagcaata attactgtga tatggataat tgttaaacta
    23821 atgaatccta atggaataaa ccccagccaa ggaaccatcg cttgtacgat gatgattgct
    23881 aacaagatcg cacgtaagac tagtcgaaag gtcttatttc tactattcat tttttcaccc
    23941 ctttttattt atactaatcg taaaaatttc tgtcactttc gtcaactaaa gaccatccga
    24001 atccgaccat ttgattagat gcgccatcca attgaaaaaa gtatgagggc aagacttgtt
    24061 tgtcttcgcc cccataatca tgtttcgttc aatgtttgaa cttttgtttt tctgtcaaac
    24121 gtaaacgccc tattgttgcg caacccattc tttcgcagct ttaagtgcat ctggaatacc
    24181 agcaggattt ttcccgccag cttgtgccat gtctggacgt ccaccaccgc caccgcctac
    24241 tttcggtgcg attgctttaa tcaagtcgcc agcttttagt ccagcttcat ttgattcttt
    24301 cgtcattgcc gctagcagac tgacttttcc ttcttgttcg tttgctaaaa caagaatatc
    24361 tgaaatattt ttttgtttcc attggtcagc taattgacgc agctgattca tatctttcac
    24421 ttttacttgt gctgcaatga ttgtttttcc attgatcgtt tctggatctt tgaatacatc
    24481 accagcttgt tgattcgcta acttgctagc taattgttca ttttcctttt gtagctctct
    24541 tacttgctgt tgcaattgtt ccgcttttga aacaacttct ttcaattgag gtgatttaac
    24601 aattgttgct acttgtttta gttgtttttc ttcttcttca agtagttcgt acgcttcttt
    24661 acttgttaca gcctcgattc gtcgcacgcc tgcaccaatt cctgattcag aaacgatttt
    24721 gaagattccg atgttttctg tatttgctac atgtgtgcct ccgcacagtt cgattgacca
    24781 gccgccaata tttactactc gaacgtcatg tccgtatttt tcaccaaaca aagccattgc
    24841 acccatgttt ttggctgtat cgatatctgt ttcaattgtt tcaactggga tagcttccca
    24901 gattttttcg ttgacgattc tttccatttc agctaattca tctgaagtga tttgaccaaa
    24961 gtgggtaaag tcgaatcgta aatgtccagg agcgactaaa gaaccagctt gatttgcatg
    25021 ctcgccaagt acttctttca atgccttgtg cagtagatgg gttgctgtat ggtttttgat
    25081 gatccgattg cgcatcgatt gatcgatcaa tagatgatag gtttgtcctt cactcattgg
    25141 tgcttttact tctacggtgt gcataaattg accattaggc gctttttgaa catctgtaac
    25201 aactgctacg atttcgccta cttcattcat gatccatcct tgatccgcta cttgcccacc
    25261 catttctgca tagaaaggtg tttgatcaaa gatcatttga gcttcacctt ctgcgacttc
    25321 attcaaaaga gactcacctt taacgatgac caataattga ctatctgatt cttgttctgt
    25381 gtatccaatg aatttacttt ctactttgat atctgtcaat aaagcagatt gtacccccat
    25441 tgatttttca gtactgcggg cagagcgagc acgttcgcgt tgtgcatcca tttctttttc
    25501 aaaaccagca tgatcaactt tcagtccttc atcttctgcc acctcttccg ttaattctac
    25561 cgggaagcca tatgtgtcgt atagtttgaa gatgtctttt cctgctagag tcgtcccgtt
    25621 ttcgtttttt acttcttgga tcacttgatt taagatttcc aaaccttcat tgattgtttc
    25681 attgaaacgt tcttcttctg tacggataac tttttcaata aattcttttt gttgaagtac
    25741 ttcaggataa taacttacca tgatttcccc aacaacaggg actaaacgat ataagaatgc
    25801 ttgttcaata cctaattttt tcccatgcat gacagcacga cgaagcaaac ggcgtaagac
    25861 ataccctctc ccttcattcg atggcaatgc accgtcgcca atagcaaatg aaagggcgcg
    25921 aatatgatct gcgattactt tgtaagaaat atctgttact gggttttcac catattttac
    25981 ttgtttgctt agcttttcta cggcgtggat aataggcata aacaagtctg tctcaaagtt
    26041 cgttgacgca tcttgaacga ttgagaccat gcgttccaat cccatccccg tatcaatgtt
    26101 tttatgagga agtggttcaa atgtgttatc aggcttatgg ttgaattctg aaaatactaa
    26161 gttccagatt tctaaataac gttcattttc tcctcctgga tagttttcag gatcgtcttc
    26221 tgctaaatcg ttataagctt ctccacggtc atagaaaatt tccgtatccg gaccacatgg
    26281 gcctgcaccg atatcccaga agttgtcttc tacatcaata atgtgatcta gtggcaggcc
    26341 aacttcttca tgccagatgc gttttgcttc tgtatctttt ggataaactg ttacataaag
    26401 tttttctgga tcgaatgcca tccattttgg atcagtcaaa aattcccatg cccagtggat
    26461 ggcttcattt ttgaaatagt caccaatcga aaaattaccc agcatctcaa acatcgtatg
    26521 gtgccgtgct gttttaccga cattttcgat atcatttgtt cgaatagatt tttgggcatt
    26581 tgtgattctt ggattttctg gcacaactga accatcaaaa tattttttca atgttgcaac
    26641 accagagttg atccacaata aggttggatc gttcacgggg atcaaagacg cactaggttc
    26701 aatcgtatgt cctttagatt ggaagaaatc aagaaacatt tgtcgaactt gtgcactact
    26761 taattgtttc atgtcattac ctgctttcta taaattaaat acaaaaaaac accatcacag
    26821 ccaaggacga aaactcgcgg taccaccttg attgcaatga tgtgtaatca ttacctctta
    26881 aactcgataa cgctgagctg cgtcggtatt tctaccaaaa tagaaaaagg gagcaatttg
    26941 attagtgact attctttttt cagccaagaa agaattttct gtgagtctta aaaaacaaat
    27001 catatccttt tcgtacttaa ttagtatacg cattctattg atcactgtca atatctcaat
    27061 ttttattttc atttcctttt ggtcatactc cacgattttt ggtacgataa gaataaagga
    27121 atcaatacat ctctgctatt catgacaagg aggaatatga tgaaaaaaat agttggagcg
    27181 gatgaatgga tacgcgcagc tagctgcttt ttgagatatc aagtttttgt attagaacaa
    27241 gggattcctg cagaactaga attcgacgga aaagatatcc cgcaacaaac ctattatcta
    27301 tttcttgatc aaaaaagacc agtagctact ttgcgttatg aaaagaaaac acctacttgc
    27361 ttcaagccgg aacgattttg cgtagcaaaa gaataccgag gaaaagggtt aggcggtcag
    27421 ttgcttgaag ctgctgaaaa acgggcggtt tcagaaggtt gtcagactag ttatgtagta
    27481 gctgaaatga cggctgtaga tttttatgaa aaacaagggt atcatacttg cacagaacct
    27541 tttattgaag atgaagtact ttgtatcgga atgaaaaaaa gtctagctta aaaacaagag
    27601 attgtgacac gccttttgtc acaacctctt gttttttatt tatgtgaatt tttcttattt
    27661 ttacgagcag tacggctttg ctgtctgcgt tcgattttgc gttctttttt atttttttct
    27721 gcaattgccc aatcgatttt tttcttataa ccaggcttga tctttttctt tttcttcttc
    27781 acaagaccaa tcagcgtggg atctaataca tcttttgatt tttctcgttt ggcacgtcta
    27841 ttacgatcgt atgttttgac gatttccccg tttttgattt cttttggttc aaatgagacg
    27901 cccaattttt ctatcgcatc aatggcttgc tcatcatttg gcgaatataa cgtaatggcc
    27961 gttcctttta agccattacg tccagtacgt ccgacacgat ggataaagaa gtctagatct
    28021 tctggaattt ctgcattaat aacatgagag acaccttcga tatcgatccc tctagcagct
    28081 aaatcagtcg ctacaacata ttgatactcc agattctgta cttgtcgcat cactcgtttt
    28141 ctctctcttg gggtgatgtc tccatgaatc tttgctactt tcaagccttg gtttttcaag
    28201 taatcggcaa tttcatccac atgctgtttt gtattggcaa atacgattgc taaataagga
    28261 tgaccaattg tcagcagttg ataaatcatt tgattctcat ctttaccttt tgttgaaatc
    28321 agccaatttt caatgtcttc agaaataact gcttttggtt tgatttcttc aatgattgga
    28381 ttttccatgt attttcgtaa gaatggacga agtttttctg gaatagtggc agaaaaaact
    28441 aaaaactgca gcttttctgg taatcgactt gcaatttggt ctacttcatt taaaaaaccc
    28501 atatccaatg tcatatccgc ttcatcgaca acaaatgcaa atgcggtgtg aatcttcagc
    28561 gcttgttcat tcatcatgtc taatattcta ccaggcgtgc ctactacaac ctgtggctgt
    28621 tggtgatgca gacgatttaa ttgacgctgt ttgtctgtac cgccgacaaa attcgagaca
    28681 cggatctccg gtgtagaaaa tttagcgatt tgtgtagctg cttcatagat ctgtgcagct
    28741 agttccctac ttggagctgt aataacaact tgtacttcct ctttttctgc attgattttt
    28801 tcaaacaaag gcaacaaaaa cgtatgtgtt tttcctgtac cagtttgaga ttgcccaatg
    28861 atattttttc cttttgcaat taaaggaatc aaacgttcct ggacctctgt gggctgtttg
    28921 aagccctttg cggttaacgc ttcattgata aacggtttga agcggaatat atcaaaatta
    28981 ttcattcgtg cacctcttct ctttaaatta tcacgacttt ggcattataa cacagacaaa
    29041 gcgtttttgc tcagctaatt tcgagaatca tcatcctttg tttggccaat cgtgctaaca
    29101 gtatatgtgt gactagcttt ttcataaaac agaatccgtt tttttctcaa aaatgatagc
    29161 aagatattta aaataaaaat accttgtagt aacaagacac cgagagaaat gaatactgca
    29221 ctcatctgca acaccgtatt cgttgtcgaa ttcatgattt gctgaagcaa ctgccccaac
    29281 cagcttaaac cataaaaaag aaagtacaga tacccgtagc ggatcatcaa tgcttttaaa
    29341 gaaattcgtt cgcggtcttc ttccaccaaa cggatacgca caattttttt gccaagtgtc
    29401 catccattgg ttataaacgg tacaatcata aagtataccc ctacttctaa taaaagccac
    29461 cagatatgag aagtaactgc ccctgaatcg aacgcaattt taaagaaaat cgccgctacc
    29521 aaattaataa gagataaaaa tgtccagtcg atcaaataag cgaccaagcg tcgagtcaat
    29581 gagacagtct ttcctttttc ataagaaagt tcatccattc gtcttcttgt aggaagcata
    29641 aatgtaaata acggcgtaac gacaaaacca atgattccac ccaatgtatt tgtcactaaa
    29701 tcattcacat caaataagcg gtatggtctt gggtagataa aataaagtcc acttagctgc
    29761 gtcaattcaa aaaatagaga aaggcagaaa ctagcaatca tcgttttcat aaaagaaagc
    29821 cggaaataat atcttaaata aatgccaaac ggtatgagta ataaaacatt aaaagctggt
    29881 tccaaaaata cactctgctt cattgccggc aggtaggtac taggttcaga caagaccaat
    29941 accgtgttgt ctctgaaatg ttgccatgaa gcaaataaat gcagctccat ctttggccct
    30001 gtcatttggg caaccgtctc ctttgccgga agtggtaaga tcaccaaaaa atacgcacac
    30061 aataaataaa aaacgaaaga atacaaaata actgcccttg ttactagaaa tgttccatac
    30121 tttcgatatt cataaataaa aaaaacagat gagatcgcta aggctaaaaa tggaaaaaca
    30181 atcagcgcaa aacgaatcgg ttcgatataa cttgccatat gactcctcct tgattctttg
    30241 tatctctctt atcttagcag tcctttgctg tttcaagcaa gtaacgaggc tattatttct
    30301 catttctttc aaagtaaagt aagataaaca caaagggggt gtcgagatgc tgaatatgta
    30361 ctttgttttt ggcgtaccta tttttttact gttcctttat gctacgatcg cttatgtaag
    30421 aaaaagaact acaattcatt atttaggatt tattctcctg attatttcgg gtttcatgct
    30481 agtcttcaat cttcagactt ggcagcaagc acttcttgaa atggataaaa tgactccaca
    30541 tgctttgtct aaagtgcttg gctaccctgt ctatctgatt tggcttccca ttttcatttc
    30601 tggttgcttg gttttattaa atatttatcg cggcgtacgc cgaatagttc agttacgcaa
    30661 atcaaagtaa aagacagaaa tcggttgagc ccgatttctg tcttttactt tgctatttca
    30721 tcgtagacaa aacgacattt acgtgatcaa aattaacgag tacttttgac atttcttcca
    30781 cgtactcctc atttaaataa aatattttgt ttggcagaga agtgcagttc taataaaatt
    30841 cgaatgtttt agctatctac gttttaaata gagataaatc gggataccag caagtgtcaa
    30901 tactatcccc atcgaagcta aaaaggtctg agtgataagc gtgcttgata cgataaaagt
    30961 ccctccaatg attgctacta gtggaataaa cggatacatt ggaactttgt atggtcgaaa
    31021 caaatcaggt tcttttttac gaagaagaat gacaccaaca aagaccatcg tataaaaaat
    31081 ccaaatgaca aagatcagca tatccgttag tgtatcaaaa ccaccgatca tcatcatgcc
    31141 aatagcgatg actagctcta agatacctgc ggctacagga actttagttt tatcatgaag
    31201 tttagcaaag agcttgctaa aaggcaggtt gttttctttt gccataacat aagggagacg
    31261 cattccagta agggtatagc cattgatggt tccataaaca gaaatcaaga taccaactgt
    31321 gaccagtctt ccaccgaagc cgccaaaaat catttttgca acgtcacttg ccgcattgct
    31381 gtttcctgcg actccgtcga tagatgcggt tcttaagaac accgcattta ctaataaata
    31441 aacgatcatg attccgatga ttcctaaaga aattgctttt ggcaaatctt tcgccggctt
    31501 tttcaattct cccgaaatat ttcctacgtg tatccaaccg tcgtatgcga acatcgttgc
    31561 tagcaagccc gctcccaagg ctgagaaaaa tgaaagattt tctcctgctt ggatcggaaa
    31621 cagttggaag tctactcctt cttgtcggaa caaaccgaaa atgacgatca caaacaaagg
    31681 aataagcttg cagacaagcg taatcgactg aaaagcccct ccagctttag aacctaaaaa
    31741 attgatcaac aggatcgata cagcagctgt aacagctacg ggaacaatca ttgactggga
    31801 aagaccaaat agattgacga actgtgtacc gaaaatgatc gacaaggctg ctacatttgc
    31861 tggaaaataa atcacgactt gcgcccagcc caataaaaaa gctgctgtat ttccgtaaat
    31921 ccgttcgata tatttaatca tccctcctgt ttctggaata gcagctgcca gttcagctcc
    31981 tgttagtcct gcacagacag aaatcatacc accgagaaac caagaaaaca tatgcaaact
    32041 ggcggatcct gtaacttctg ctacgcttgc ggctttaaaa aatacccctg caccgatcac
    32101 tgtgcccata acagtcgata aagcagtaaa aaatcccatc gtacgtttta attcattttc
    32161 tatttttact tcttttactg gcgtggcttg atattcttcc atcccttgtc ccctttcata
    32221 ccatagccaa tcccaaaagt ataaaaaact ttttggatgt ggttcttgtt ttataccgtc
    32281 tctaccaatt tttctttcag tttttccaac atatcctttg tcatcacatc taagtcgtat
    32341 ttcggcgaaa agccccattc ctcttgcgca caagaaacat ctagtgaatt tggccaagat
    32401 tctgctattg actgacgtac aggatccaca tcataatcca tttcaaaatc tggtatgatt
    32461 ttttgaatag atgctttgat ctcttccggg tcaaaagaca tcgccgatat attgaaggca
    32521 tttcgatgaa ccaatcgatc aggatctgca ttcatgagtt ttataatggc atcgatcgca
    32581 tctggcatat acatcatatc cattgatgta ccttgatcaa tgaaactagt gtactttcct
    32641 tctttgattg ctgaataata aatatcgact gcgtagtctg tcgtaccgcc tcctggcaag
    32701 gttgcataag aaatcaatcc agggaaacgg acaccacgag tatctacgcc atatttcaca
    32761 taataataat cacatagtag ttctccagct accttcgtta ccccatacat ggttgtagga
    32821 cgctgaagag tatcttgtgg ggtatgatct tttggagtat ttggaccaaa cgcgccgatt
    32881 gaacttggcg taaaaaattt cagctggaat tctctagcga cttccaatgc attgaccagc
    32941 ccagtcacgt tcaaattcca ggctaattgt ggttttgctt ctgcaacagc tgacaaaaga
    33001 gcagccaaat gaatcaatgt gtccacttga tattgttctg ctatgctgcg cattttttcg
    33061 taatccatta catctaggat ctcaaagata tcgtttttct caatgacttc aggcatgcgt
    33121 atatctgtcg caatgacatt ttcactgcct agttcttgac gtagccgtgt gatcaactct
    33181 gaaccaattt gtcctaaaca tcctgtcacc aatacttttt tcatgatgat cctccctgat
    33241 cttttttact actatcttca gatgattaac aatatatgcg tgaacatgtt ctctaatttt
    33301 aaatagacga aaaaactaaa tttagttaaa taatttccat ttcttttcct attttttgat
    33361 aaactgctac tgcttcatca agcatttccc tcgtatgttc cgcagtcggc atatttctga
    33421 tcctgcctgt cccaagaggc acagttggat aaacgattgg tttagcataa atcccatgtt
    33481 cgatcagctt cttagagaaa gtctgagtca ctttttcgtc tcccaaaatg actggagtaa
    33541 taggtgtttc agacataccg atgttatagc ctactttttt caattcttct ttaaaataat
    33601 tagcattttc ccaaactttt tcgacaagtt ccggatgttc ttgcatcaaa gtaatcgaag
    33661 ccaatgcagc tgcagctgca cctggagtta gcgaagtaga aaataggaat ggccgacttc
    33721 tagctttcaa ccagtcgatt agtgttttcg atccagctac ataaccccca acaacaccta
    33781 tagctttcga taacgtgccc atttgcatat cgattttgtc gcttaatcca aaatgcttga
    33841 cggttcctgc accttttcca gttacgcctg agccgtgcgc atcatcgacg taggtgatca
    33901 gtccatattt ttcaacaatc ggaataattt ccggcaatcg tgcaatgtct ccatccatgg
    33961 agaaaacacc atctgtgata tacatgattt ttttatattt cttactttct atcgcttcct
    34021 ttgctttttt ctcaaggtct ttcatatctt gatgtttaat tcggatgatt tttgctccgg
    34081 ataagcggca accgtcaata atagatgcat ggtttaattc atccgataag atagcatctt
    34141 cttttgtcat tactgcagag atagcgccca tattgcagtt gaatccagat tgaaaggcga
    34201 ttgctgcttc tgtccctttg aattctgcga ttttttcttc taattgctga tggattttca
    34261 gcgaaccatt gatcgttcgg actgctcctg ctcctacacc ataagtttcg gttgcttcaa
    34321 tacatgcttt tttcaattct tcgcgattgg caaatccaag ataattgttg gatgctagat
    34381 tgatcatctt ttttccgtct acgatgatac aagcaccatt ttcactttct agtatgtcga
    34441 tcgtattgta taaccccttt tctttcagtt gatccaattc tttctctaaa tacattgtta
    34501 gttctttgga agacattgtc tccacctcca tattcatctg tcttgcagaa cataccaaac
    34561 atatatcgtg tcattcatct acaaagcaag tatgctatat ttttgataac gcttacactg
    34621 attatgagaa ttatttaacc acatttttta tttacagtat ctttttactt tcaaatgact
    34681 tatatagtat gaacaattac cttttagata atgatctatt ttactctacc tgggcagata
    34741 aatataacac aaccattgat agaagccctt tcgttaaaat caattgtatt ctgaatattt
    34801 aatttattta ttgcatagac caataaaaat acataaaatt tccatcttcg tttatcagca
    34861 atttttaatt ttactctctg aatatttctt aaaagtttta cttgttgaat tcgaaaaaag
    34921 gactgtgaca aaagtcttgt cacagtccta ttcttccgaa ta
//
LOCUS       DAZBTF010000023        34424 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000023 DAZBTF010000000
VERSION     DAZBTF010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.023"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            352..1041
                     /locus_tag="ACHGNS_001198"
     CDS             352..1041
                     /locus_tag="ACHGNS_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFN6937158.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="ACHGNS_001199"
     CDS             1028..2236
                     /locus_tag="ACHGNS_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFN6937159.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="ACHGNS_001200"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="ACHGNS_001200"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFN6937160.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACHGNS_001201"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACHGNS_001201"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFN6937161.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6339..7034)
                     /locus_tag="ACHGNS_001202"
     CDS             complement(6339..7034)
                     /locus_tag="ACHGNS_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFN6937162.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="ACHGNS_001203"
     CDS             complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="ACHGNS_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFN6937163.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8233..8424)
                     /locus_tag="ACHGNS_001204"
     CDS             complement(8233..8424)
                     /locus_tag="ACHGNS_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFN6937164.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8445..9335)
                     /locus_tag="ACHGNS_001205"
     CDS             complement(8445..9335)
                     /locus_tag="ACHGNS_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFN6937165.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9322..10089)
                     /locus_tag="ACHGNS_001206"
     CDS             complement(9322..10089)
                     /locus_tag="ACHGNS_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFN6937166.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="ACHGNS_001207"
     CDS             complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="ACHGNS_001207"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFN6937167.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="ACHGNS_001208"
     CDS             complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="ACHGNS_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFN6937168.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="ACHGNS_001209"
     CDS             complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="ACHGNS_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFN6937169.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14660..15043
                     /locus_tag="ACHGNS_001210"
     CDS             14660..15043
                     /locus_tag="ACHGNS_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937170.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15211..15969)
                     /gene="jag"
                     /locus_tag="ACHGNS_001211"
     CDS             complement(15211..15969)
                     /gene="jag"
                     /locus_tag="ACHGNS_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFN6937171.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15987..16805)
                     /locus_tag="ACHGNS_001212"
     CDS             complement(15987..16805)
                     /locus_tag="ACHGNS_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFN6937172.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="ACHGNS_001213"
     CDS             complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="ACHGNS_001213"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFN6937173.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="ACHGNS_001214"
     CDS             complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="ACHGNS_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFN6937174.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17933..19267
                     /gene="dnaA"
                     /locus_tag="ACHGNS_001215"
     CDS             17933..19267
                     /gene="dnaA"
                     /locus_tag="ACHGNS_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFN6937175.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19465..20595
                     /gene="dnaN"
                     /locus_tag="ACHGNS_001216"
     CDS             19465..20595
                     /gene="dnaN"
                     /locus_tag="ACHGNS_001216"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFN6937176.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20824..21066
                     /gene="yaaA"
                     /locus_tag="ACHGNS_001217"
     CDS             20824..21066
                     /gene="yaaA"
                     /locus_tag="ACHGNS_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFN6937177.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21053..22177
                     /gene="recF"
                     /locus_tag="ACHGNS_001218"
     CDS             21053..22177
                     /gene="recF"
                     /locus_tag="ACHGNS_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFN6937178.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22174..24120
                     /gene="gyrB"
                     /locus_tag="ACHGNS_001219"
     CDS             22174..24120
                     /gene="gyrB"
                     /locus_tag="ACHGNS_001219"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFN6937179.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24140..26611
                     /gene="gyrA"
                     /locus_tag="ACHGNS_001220"
     CDS             24140..26611
                     /gene="gyrA"
                     /locus_tag="ACHGNS_001220"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFN6937180.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            26847..27146
                     /gene="rpsF"
                     /locus_tag="ACHGNS_001221"
     CDS             26847..27146
                     /gene="rpsF"
                     /locus_tag="ACHGNS_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFN6937181.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27196..27708
                     /gene="ssb"
                     /locus_tag="ACHGNS_001222"
     CDS             27196..27708
                     /gene="ssb"
                     /locus_tag="ACHGNS_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFN6937182.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27734..27970
                     /gene="rpsR"
                     /locus_tag="ACHGNS_001223"
     CDS             27734..27970
                     /gene="rpsR"
                     /locus_tag="ACHGNS_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFN6937183.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28113..30086
                     /locus_tag="ACHGNS_001224"
     CDS             28113..30086
                     /locus_tag="ACHGNS_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFN6937184.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30092..30544
                     /gene="rplI"
                     /locus_tag="ACHGNS_001225"
     CDS             30092..30544
                     /gene="rplI"
                     /locus_tag="ACHGNS_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFN6937185.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30818..32185
                     /gene="dnaB"
                     /locus_tag="ACHGNS_001226"
     CDS             30818..32185
                     /gene="dnaB"
                     /locus_tag="ACHGNS_001226"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFN6937186.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            32965..34257
                     /locus_tag="ACHGNS_001227"
     CDS             32965..34257
                     /locus_tag="ACHGNS_001227"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFN6937187.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10506 a         6694 c         6817 g        10407 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtgtattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6301 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6361 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6421 ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc
     6481 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6541 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6601 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6661 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6721 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     6781 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     6841 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     6901 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     6961 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     7021 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7081 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7141 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7201 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7261 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7321 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7381 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7441 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7501 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7561 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7621 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7681 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7741 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     7801 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     7861 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     7921 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     7981 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     8041 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8101 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8161 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8221 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8281 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8341 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8401 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8461 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8521 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8581 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8641 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8701 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     8761 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     8821 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     8881 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     8941 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     9001 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9061 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9121 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9181 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9241 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9301 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9361 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9421 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9481 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9541 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9601 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9661 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9721 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
     9781 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
     9841 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
     9901 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
     9961 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
    10021 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10081 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10141 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10201 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10261 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10321 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10381 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10441 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10501 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10561 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10621 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10681 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10741 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    10801 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    10861 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    10921 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    10981 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    11041 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11101 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11161 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11221 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11281 tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc
    11341 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11401 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11461 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11521 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11581 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11641 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11701 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    11761 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    11821 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    11881 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    11941 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    12001 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12061 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12121 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12181 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12241 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12301 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12361 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12421 ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12481 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12541 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12601 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12661 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12721 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    12781 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    12841 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    12901 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    12961 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    13021 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13081 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13141 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13201 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13261 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13321 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13381 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13441 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13501 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13561 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13621 caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa
    13681 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13741 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    13801 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    13861 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    13921 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    13981 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    14041 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14101 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14161 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14221 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14281 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14341 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14401 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14461 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14521 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14581 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14641 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14701 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    14761 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    14821 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    14881 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    14941 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    15001 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15061 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15121 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15181 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15241 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15301 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15361 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15421 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15481 gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat
    15541 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15601 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15661 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15721 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    15781 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    15841 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    15901 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    15961 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    16021 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16081 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16141 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16201 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16261 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16321 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16381 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16441 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16501 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16561 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16621 aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16681 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16741 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    16801 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    16861 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    16921 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    16981 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    17041 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17101 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17161 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17221 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17281 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17341 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17401 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17461 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17521 aacttatcca caagattatt tttttctatg ggaatcctat gtggaaaaag ttatgcacag
    17581 ggattatcca cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17641 aatccacaat caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt
    17701 ttttatccac agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt
    17761 agtggtgtgg aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg
    17821 tgtggataat tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa
    17881 aatgctaaat tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc
    17941 cctcgatgct ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag
    18001 ctataataca tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt
    18061 tgaggttcct agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt
    18121 tgaagtcggt tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga
    18181 agcagagcaa gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct
    18241 aaaaaaagcc atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa
    18301 ccagatggct catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc
    18361 gctcttcttt tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca
    18421 tcaaatgctg caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc
    18481 taatgatttc atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg
    18541 aaatgttgat ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca
    18601 agaagagttt ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac
    18661 cagtgatcgg ttgccaaacg agattccgaa actgcaagaa cgccttgttt ctcgctttgc
    18721 atgggggctg tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg
    18781 taaaaaagcg gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg
    18841 acaaattgat tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc
    18901 tgcgatgaat agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa
    18961 atcaggaaaa ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta
    19021 ttatcacatc caattgaaag atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc
    19081 tagacaaatc gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg
    19141 agctgaattc ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca
    19201 gctgatggat tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact
    19261 aaattaattt gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc
    19321 aaaagttata cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt
    19381 tttccacaga atcaacatgc cctattactt ttattattct ttttaactaa taataaataa
    19441 acaaacattc aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag
    19501 gaattgcaaa ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt
    19561 gtaaaaatca cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca
    19621 attgaaactt ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt
    19681 tccattgttt tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca
    19741 tttactttag aagttttaga aaataaacaa gtagcgatca cttctggaaa agcgaatttt
    19801 atcgtaaatg gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac
    19861 cagatgaaat tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc
    19921 tcacaacatg agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct
    19981 ttattagctg tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa
    20041 gcggctgatc attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca
    20101 ttaacaaatg aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa
    20161 acagaaacga tgtatttcta ttcccgtttg ctagaaggaa actatccaga taccaatcgt
    20221 ttgattcctt caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg
    20281 atcgaacggg cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt
    20341 cgtccagatg cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc
    20401 ttgagttata cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg
    20461 aaagcagcgt tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt
    20521 cgtccattta cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct
    20581 gtacgtacaa attaacaatg attttcaatg ttcttgaaaa tttaattgaa aagaaataga
    20641 aaaaataaaa ttttaaacag aaggccctaa aatctctttt aaggctttct gtttattttt
    20701 gtttaaatca ccttttttag attaaatcgt cttatatgga aaaatatatg gataaatttg
    20761 ttttattgcg aaaaaaaagg tataatagag tatactttta ataatgaaaa tgagggatgg
    20821 gcattgaaac aacaagtcat tctaaatacg gaattcatga cgttgggcca aatgttgaaa
    20881 gaagtcacag ttatcggtag tggcggacaa gcaaaatggt atttggcaga aaacactgtg
    20941 ttagtcgatg gcgaaccaga aaatcgtcgt ggacgcaaac tttatcccgg catgatggtt
    21001 gaagttccag aagtaggaac tttttttatg gtaaaaaaag gaaacgacga taatgaggct
    21061 gaataagctg tatttaaaaa attatcgaaa ttacgaagag ctgaacatcg aattttccaa
    21121 gaacctcgtc atctttttag gagaaaatgc gcaaggaaaa acgaatcttt tggaaagtat
    21181 ctacgtatta gctatgacgc gaagtcatcg gacaagtagt gagcaggaat tgattggctg
    21241 gactgacgat caggcgatga ttcaaggaga gatcacaaaa ggttcatcca ctcttccatt
    21301 agaaatctta ttatctaaaa aaggccgaaa aaccaagatc aaccatatcg aacagaaaaa
    21361 gttaagtact tacgtgggac aaatgaatgt cattttattt gcaccagaag atctttcttt
    21421 agtaaaaggc agcccgcaga tcaggcggaa gtttttagat atggaattag gccagatcaa
    21481 tccaatctat ctttatgatc ttgtccaata ccaagcaatt ttgaaacaaa gaaaccagta
    21541 cttgaagcaa ttgaatgaaa aaaaacaaac tgatttgctt tatttggata ttttgtccga
    21601 acaactagca gcgtttggca gcaaagtatt gaaagcaaga gcacagttcg tcaagcgatt
    21661 ggagttttgg gctaattctt tgcatcagca aatcacccat caaaaagagc agctggaaat
    21721 cgagtatctc accgcagtcg attctttaga aacacatacg caagagcaaa tccaagaaca
    21781 atttctcgca cttttgaacc aaaataaaaa gaaagacctt tttcggggaa caacgacagt
    21841 tggtcctcat cgtgacgatc tttccttttt tattaatcag aaaaatgtgc agacttatgg
    21901 atcgcaagga cagcaaagaa caactgcact aagtgtcaaa ttagcagaaa tcgatttgat
    21961 taaagaagag acaggggaat atccaatcct tttgttagat gatgtgatga gcgagctgga
    22021 tgataatcgt caactccatc tgctagaaac aatcgaagga aaagttcaaa cgttcttaac
    22081 aacaacgaca cttgaccatg taaaaaataa aatgactgtt gaacctgaga ttttttatgt
    22141 tcaacaaggg aaactaaaag gagaagaaac tgaatgacag aagaaagaag tttagtagaa
    22201 cgcgctaaag agtatgatgc cagtcagatt caagtacttg aaggacttga agccgttcgt
    22261 aaacgacctg gcatgtatat cggttcgact agttctgagg gtctgcatca ccttgtttgg
    22321 gaaattgtcg ataattcgat cgatgaagta ttagcaggtt ttgcaacgaa aatccatgtg
    22381 attattgaaa aagacaacag tatcaccgtt attgacgacg gtcgggggat acctgtagat
    22441 atccaggcaa aaactggacg tcctgcagtt gaaacggtct ttactgtttt acatgctgga
    22501 ggaaaattcg gcggtggtgg gtacaaagtt tctggtggac ttcacggagt aggatcttct
    22561 gttgtaaacg cgctttctac acagcttgat gtcaaagtat acaaagaagg aaatgtgtac
    22621 tatcaagaat atcgtcgtgg agctgtagtg gacgacttaa agattatcga acaaacggat
    22681 cgccatggaa caacggttca ctttacgcca gaccctgaaa tttttacaga gacaactgag
    22741 tttgattttt caaaattggc tacccggatc cgtgaacttg ctttcttgaa ccgcgggatg
    22801 cgtatatcga ttgaagacaa acgagaagaa gaaccagtca tcaatgaata ccattatgac
    22861 ggcgggatca aaagttatgt cgaatacttg aatgcaaaca aaaccgtgat ttttccagaa
    22921 cctgtatatt tggaaggtga acagcaagat atcgcagttg aagtctctat gcaatataca
    22981 gacggctacc attccaatat catgagtttt gccaacaata tccatacgta tgaaggtgga
    23041 acacatgaat caggattcaa gactgcttta actcgtgtga taaatgatta tgcccgaaaa
    23101 caaaagctga tgaaagaaaa tgacgacaac ttgaccggtg aagacgtgcg agaaggattg
    23161 acagctgtta tttctatcaa gcatcctgac cctcaattcg aaggacaaac aaaaacaaaa
    23221 ttgggcaatt ccgaagtacg gacagtcact gatcggttat ttagcgaaca tttcatgaaa
    23281 ttcttgctgg aaaacccaag tgtgggccgg caaattgttg aaaaaggatt actagcttcc
    23341 agagctcgtc tagcagcgaa acgtgcgcgt gaagtaacga gacgtaaagg tgctttagag
    23401 attagtaatc tgccaggaaa attagcggac tgctctagca acgatcctga aaaatgcgaa
    23461 ttgttcatcg tcgaaggaga ttcggccggc ggatcagcta agcaaggtcg taatcgagag
    23521 tttcaagcca tcttgcctat tcgtgggaaa atcttgaatg ttgaaaaagc aagcatggac
    23581 aagatcttgg caaatgaaga gatccggtct ttatttactg ctatgggtac agggtttgga
    23641 gaagattttg atgtatcgaa agctcgctac cacaaattag ttatcatgac tgatgccgat
    23701 gtcgacggcg cgcatatccg tacattgctg ctgactcttt tttatcgtta catgcgtcct
    23761 attgttgaag ctggatacgt ctatattgct cagccacctt tatacggggt aaaacaaggg
    23821 aagaatatta cgtatgtaca accaggaaaa aatgcagaag aagagttgaa acaagtcgtt
    23881 gcttcacttc ctgcctcacc aaaaccaagc gttcagcgtt ataaaggact tggagaaatg
    23941 gatgatcatc aactatggga aacaactatg gacccaagca atcggatgat ggcccgcgtc
    24001 agtgtggacg atgctattgc agcagatcag atatttgaaa tgttgatggg ggatcgagta
    24061 gaaccccgtc gtgcattcat cgaggaaaat gcacactatg tgaagaactt agatatttaa
    24121 tcatgaagga ggaaaaagaa tgagtgaaga aatcagagaa aacatccatg atgtcaatct
    24181 aaccagcgaa atgaaagact ccttcatcga ttatgcgatg agtgttattg tcgcacgtgc
    24241 acttcctgat gtcagagatg gtctgaaacc cgttcatcgc cgtattttat acggcatgaa
    24301 cgaattaggt gtaacaccgg ataaagccca taaaaaatct gccagaatcg tcggagatgt
    24361 catgggtaaa taccatcctc atggagacat tgcgatctat gagtcaatgg tacggatggc
    24421 acagccattc agctaccgtt atatgctagt ggatggtcat ggaaactttg gttctgttga
    24481 cggtgacggt gccgccgcta tgcgttatac cgaagcacgg atgagcaaaa tcgctacaga
    24541 aatgctgcgt gatatcaata agaacactgt tgatttccaa agcaactatg atgatacaga
    24601 aaaagaacca gttgtactac ctgctcgctt cccgaatctt ttagtgaacg gaacaacggg
    24661 gatcgccgta ggtatggcca cgaatatccc acctcataac ttgtctgagg tagtagcggc
    24721 tatcgatttg ctgatggata atcctgatgt gacgaccaat gaattgatgg aagtattacc
    24781 tggaccagat tttccaacag gtggtttggt tatggggaaa tcaggaatcc gaagagcgta
    24841 tgaaacaggt aagggttcta ttaccgtacg tgcaaaagtt gagattactg agatgccaaa
    24901 tggtaaagaa cggatcatcg tgacagaatt gccttatatg gtcaataaag caaaattgat
    24961 cgaacggatt tctgaactgc acagagacaa gcgtatcgaa gggatcacag atttgcggga
    25021 cgaatcttcc cgtgaaggta tgcggatcgt cattgacatc cgtcgtgacg ccagcgcatc
    25081 cgttatcttg aataacttat ataaattgac atccttgcaa aattcatttg gattcaatat
    25141 gttggcgatc gaaaaaggtg tgccgaaagt tttgagcttg aaacaaatcc ttgaaaacta
    25201 tatcgaacat caacgcgaag tcatcacacg acggacggtc tttgaaaaag aaaaagcaga
    25261 agcaagagca cacatcttag aaggtttgcg tattgctttg gatcatatcg atgaaatcat
    25321 tgcgattatc cgcgggtcta aatcagatga cgaggcaaaa gcaacaatga tcgaacggtt
    25381 cgatttatcc gatcgccagt ctcaagctat tttagatatg cgtttacgcc gattgaccgg
    25441 tttggaacgg gaaaaaatcg aaaatgaata ccaagaactc ttaaaactga ttgaagattt
    25501 aactgacatt ctggcaagac cagaacgtgt tacagaaatc atcaagaatg aattagcaga
    25561 cttgaatcaa cgatttggtg ataaacgacg cacagaatta ctagtcggtg aggtcttaag
    25621 tctggaagat gaagatttaa tcgaagaaga agaaatcgtt attacattga caaacaatgg
    25681 ttacatcaaa cgtttggcaa ataatgaatt cagagctcaa cgaaggggtg gacgtggtgt
    25741 ccaaggaatg ggtatccaca atgacgactt cgtcaaaaat ctggtttctt gttctaccca
    25801 tgatacattg ttattcttta cgaataacgg aaaagtgtat cgggcaaaag gatacgaaat
    25861 tccagaatac gggcgtacag ccaaaggaat cccaatcatc aatttactag gtatcgattc
    25921 gagtgaaagc atccaggcaa tcattgcggt gacgggagaa gcagaagaag gccactatct
    25981 cttctttacg acgcgtcaag gtacagtaaa acgtacttct gtcactgctt ttgctaacat
    26041 ccgaagtaat ggtctgatcg caattggttt gaaagaagac gatgagctcg tcaatgtgct
    26101 actgacagat ggtcaatcta atatcattat tggaacacat aacggctact ctgtcacttt
    26161 caaagaagaa gcagtccgcg atatgggacg tacagcttcc ggggtcagag ggatacgcct
    26221 gcgtgaggaa gattatgtcg ttggggcagc cttaatgaag gaaaatcaag aagtattagt
    26281 gatcacagaa aaaggttacg gaaaacggac aaaagtatcg gaatatccag tgaaaggccg
    26341 cggaggtaaa gggatcaaaa cggcgaacat cactgaaaaa aatggtccac tagctggatt
    26401 gaccactgtt tctggagatg aagacatcat gctgattaca gataaaggtg tcattatccg
    26461 tttcaacgta tcgactgttt ctcaaactgg acgttcaaca ttaggcgtac gtttgatgaa
    26521 gatggaagct gatacgaaag ttgtcacgat ggcagcagtc gaaccagaag ctgcagaaca
    26581 aacagttgaa gaacaaggat cagaagaata accatcaaga ctaagtgtgt aagctagcaa
    26641 gaataaccga ttaaaaaatc tggttattcc tgctggcttt tttgattagt tgaatttctc
    26701 ttaaaatatt cgaaactgtt cattctgtat tgcttttgac caaaatagag tgtataatta
    26761 gagcttgtga gtagtctaat taattagata actctccttg ctcttaggaa cctaagagcc
    26821 gaaagtccac agggaggtga atatcaatgg aaaacacgaa atatgaaatc atgtacatta
    26881 ttcgtccaaa cattgatgaa gaagcaaaaa ctgctttaat cgaacgtttc gatgcaatct
    26941 tgaaagataa cggagcagaa gttatcgaat caaaagattg ggaaaaacgc cgtttcgctt
    27001 atgaaatgaa cggttaccgt gaaggtatct atcacatcgt taaagtatct tctccatcat
    27061 cagcaaatgc tgtcaatgaa tttgaccgtc ttgctaaaat caacgacgat atcattcgtc
    27121 acatgattgt taaagaagaa gcttaattgt tgtttcatgt gaaacaatag aaaacgatga
    27181 gaggagatga ctattttgat taataatgtt gtattagttg gacgtctgac gaaagatcca
    27241 gatttgcgtt acacagcaag tggtacggca gtcgcaacat ttactttggc tgtgaatcgt
    27301 aattttacaa accaaagcgg caatcgcgag gctgatttta tcaactgtgt gatttggcgc
    27361 aaatcagcag aaactttagc aaactatgct cgtaaaggaa cacttttagg tgttactgga
    27421 cgtatccaaa ctcgttctta tgataatcag caaggacaac gtgtatacgt gacagaagta
    27481 gtagctgaca acttccaatt gctggaaagt cgttctgcat ctgaaaatcg ccaacaaagc
    27541 ggcggattcc aaagttctgg tcaatcagct ggcggatttg gcggaaacaa taactctaat
    27601 caaacatctc aatcatccaa cggtatgcct gatttcgacc gtgatacttc tgacccattc
    27661 ggctcaagtt caacgatcga catttcagac gatgatttac cattctaaac gaatttgata
    27721 ggagggaaat agaatggcac aacaaagaag aggcggacgt aaacgtcgta aagtagacta
    27781 cattgctgct aaccatattg aatatatcga ttataaagat atcgaattac taaaaagatt
    27841 tatttcagaa cgcggtaaaa tcttgccacg tcgtgtaact ggtacaggcg ctaaaaacca
    27901 acgtaaaatt actgttgcaa tcaaacgcgc tcgtatcatg ggcttgctac ctttcgttag
    27961 cgaagactaa taaatcctag gaattgaatg actgatcctt ttgatcagct attcaattcc
    28021 tttttttctg taaaaataac ctctattcgt ttagtcgctc gaatgacgaa agttatggta
    28081 aaatcaaatt agtcatatat gggaggaaaa ttatggaaaa taaaaacaat catttatccc
    28141 cgaaacctct cttccttatc ctgtttatat taatcgaagt attggtagtc ttaattttac
    28201 catttcgttt tgttagcgct tcggtcattc tattgatgaa tttatggggt gttcgctatt
    28261 ggttgaaaat gaaacgttgg cttattttaa ataaacgtga aaaaatccag caggcttcga
    28321 ctgtagctga aaccaatcta gcttatgtct cagaagtcat gcctgtagga gtgattgctt
    28381 atcagccatc aagccaaaaa atcgaatggc tcaatccatt agcattgaaa atcaaagatc
    28441 aaagtggatt aacagaggaa gaattgctag atactttttt tactgcaaaa gaaaaaaaga
    28501 aaaaccaagt ctatctagca gataaaacat ttcagtttga tatggataca gtaaagggtg
    28561 ttgtttattt taatgatatt acccaaaacc agttgttaaa actccatcag acgaacagtc
    28621 agccggtgat tggtatttta tctattgata actatgcaga tgccattgat aaattagatg
    28681 acaaagagat ttcttatttg aactctttcg tcactacact agtttctgac tggatgaatg
    28741 aataccatgt ctttttcaaa agaatcaacg cagaacggtt tttcttcgta gcacagatga
    28801 tcgatctgaa aaagatgatg gataaaaaat ttgatttgct ggatcgcttt cgagcagaag
    28861 ctgaaaaaca gagctcgcct atcaccctta gtatggggat tgcttatggc tcaagaaccc
    28921 tcgatgaaat cggtggagaa gctcagaata atttagatgt tgctttagtc cgtggaggcg
    28981 accaagttgt cgtgaaagag gtaaaagaag gagcaaaacc tgtttattat ggggggaaat
    29041 ctgctaagaa tgtcaaacgc acgcgtgttc gttctcgagc gatgagtacc gctttgcgtc
    29101 ggatttttga tgaaacaggc gatgtgttta tcatggggca caagtttccg gatatggatg
    29161 ctttaggctc tgcttttggt gtcagttgtc tggcacgctt caaccagaaa aaagcttaca
    29221 tcgttatcaa tgagaacgag atcattccag atgtagaacg gtgtcttgaa gaaatccata
    29281 agcatcctga attagaaatg caactaatct ctccagaacg tgccatagag ctgaaaaaag
    29341 aagaggacct cctcgtgatg gtggattacc ataaaccttc tctatcgatc tctcagggcc
    29401 tatatgagca atttgataag attgtgatca ttgatcacca tcgtcgtggg gaagagtttc
    29461 caagtaaacc tctgctgact tatattgaat cttcagcttc ttcagcttca gagctagttt
    29521 ctgagctgat ccaataccaa agcagcaaaa caaatcgctt agatcgtttt gaggccacat
    29581 tattgcttgc agggatggtt gtagatacaa atagtttcaa tgtccgtaca actgcacgaa
    29641 cttttgacgt tgccagctat ttaaggtctt gtggagcaga cgcatcctta gtacaatatt
    29701 tattgagctc ggatttagcg acatacttgg agatcagcaa tttgattgct ggaagcgact
    29761 atgtgacttc tgatattgtt gtggcggcag ggacagacga aaaagagtat aatagtgtta
    29821 tagcagcaaa aacagcagat acattgcttt cgatgatcaa tatcaatgca gctttcgtca
    29881 ttacaaagcg gacagacggc ttgattggga tcagtgccag aagtaccggc acgatcaacg
    29941 tccaaatcat catggaatcc ttaggtggcg gaggacactt cacaaacgca gctactcaac
    30001 tggaaaatac gacagttgag gaaacaaaac agaagctatt tgctgcaatc aaagaaaata
    30061 tgaatcaaat atatgataag gagtaatcca aatgaaagta atttttttac aagatgtaaa
    30121 aggaaaaggt aaaaaagggg acatcaaaga agttccagca ggttatgcac aaaattatct
    30181 aatcaaaaat aacttggcaa aagaagcaac gaaaagcagt atcagtcagt tagcaggaca
    30241 aaagaaagca caagaaaaac acgaagctga aatcttggaa gaagctaaaa agttaaaaga
    30301 attcttggaa aaagaagata ctgtagtcga attgaaggcg aaagcagggg aagacagccg
    30361 tttgtttggt tcgatccctt caaaacaaat tgctgaaggg ttaaacaaac aatacaaaat
    30421 caaattagat aaacgaaaaa tcgaattaga aaatccgatc cgttcactag gctacacaaa
    30481 cgtacctgtt aagcttcatc atgaggtgac ggctacgatc aaagttcatg tcgtacaaga
    30541 ataacaaatc ttgatagcga tgttcagctg gtaagtataa ccagaaggaa aaaagacaaa
    30601 ccgaaattta tctgagaaaa cagctgcagc aatgaaagca aaagctgcaa atcgatttca
    30661 gattgatttc ggtttctttt ttgggaacag caaaatagca taaagaaaac gagtataata
    30721 aaagagaact atttttttat tacactttcg gtacgaatga tgacttttct ctcattttct
    30781 tgtaaaatga agcaattgaa gacaaggagc gatacgtatg aatgaagtgt ggcaggatcg
    30841 aatcccaccg caaaatattg aagctgaaca agcggtttta ggctctgttt ttcttgacgc
    30901 agaaacgatc attgatgcca tggagtacat agagccaaaa gacttttatc gtcgtggcca
    30961 tcaaattatt tttgagacaa tgatcgagtt aaatgatcgt aatgaagcga ttgacgtggt
    31021 tactgtcaaa gaccggctgg aacaagccaa tttgttagaa gatgccggtg gtttaagtta
    31081 tctctcagac ttggcattag cagttccgac agcagcaaat atcgtctact atgcgaaaat
    31141 cgttgaagaa aaatcattgc tgcgtacatt gatccagaca gcaacaggaa tcgttacgaa
    31201 aggattcgag caaggagaag aagttcaaac aattttagat gatgccgaac gcagtatctt
    31261 ggaggtttcg gaaaaaagaa atcgaagcgg ctttttatct attgctgaag tactgaactc
    31321 cacgattgaa aatatcgatc gactagctca aaacaatgaa gaaatcacag gtctaccaac
    31381 tggctatcaa ggtctggata aaatgacagc aggtttccag cccgaagaac taatcattct
    31441 tgctgcacgt ccagcggtag gtaaaacagc gtttgcattg aatattgctc aaaatgtagg
    31501 aacaaaaaca gatcgttctg ttgcgatatt tagtttggaa atgggggcag aatctcttgt
    31561 aaatcggatg ctttgtgcag aaggttcgat tgaggccagc catttacgta caggtcagct
    31621 ttcggaagaa gaatggtcga atctgatcgt tgcgatgggg agtctttctc gagcaaatat
    31681 ttttatcgat gatacaccag ggatcaaaat ctctgaaatc cgtgcgaaat gtcggaaact
    31741 agcgcaagaa aaaggcaatc taggcttgat attgatcgat tatttgcagt tgattgaagg
    31801 taacggtaaa gagaaccgtc aacaagaagt atcagatatc tctcgccaac tgaaaaaact
    31861 ggcgaaagag ctgaaagtac ctgtcattgc tctttcacag ttatctcgtg gagtagaaca
    31921 gcgacaagac aaacgaccag tgctaagtga tattcgtgaa tccggatcaa ttgaacaaga
    31981 tgcggacatc gtagccttct tgtatcgaga tgattattat gaaagagaag gcggagatga
    32041 cgatgatgga aacgagccaa aaaatgacaa cgtcatcgaa gtcatcatcg agaagaaccg
    32101 aagcggtgca cgaggaacag ttgaactact gtttatcaaa gaatacaata agttttcgtc
    32161 gctttcgcca agagaagaat tttaaagaag aggttgtgag ccttgagcaa tcccgagagc
    32221 tctcgtaaga agatgcaagg agtaaccgca ggaacgacta gagtacttaa tttaaacaga
    32281 tctccaaagg gattgtgaca aaagtcttgt cacagtccct ttttccgaat aaacggtgtt
    32341 caatcagctg acccttggta ttagctcagc aaacgtcaaa atatgaggaa ctattttgac
    32401 gtttgtttct ctaatactca ggtcaaaacg ctgatcttac gacctctttt ccttaaacgg
    32461 tgttctcctg ccaactcctc ggtcacaagt cacaatatca tcaaacatga gaagcagttt
    32521 tttgaaattg ctccttgtga ctcagagtta aacggcaggc tcacaacctc tttcttaaac
    32581 ggtgttcaaa agcttgcatc tgcggtaata cgcccaatca aacgaaaaat atgagaagct
    32641 atttttgttt ggctgttctt attacttgat gcaggacagc tttttcacaa cctctgattc
    32701 acggtgttcc aaaagtagct tcttcgaaaa ataagtcgaa atttcacaaa aatttgagaa
    32761 gcaattttcg tacattcctt cttatttctt gaagctgacc acttctgtta caacctcttt
    32821 tttaataacc attctaaaaa gataaagttc gttttttaag cgtttttata aaataaaaat
    32881 aaaaatcatt cgattttatt tgaaatatgt atctattatt gatacaatgg ttcaggatta
    32941 aataatatag aacgaggtgt tcacatgtca tctgtagtag tagtaggtac gcaatggggc
    33001 gatgaaggga aaggaaaaat cacagacttt ctcagtgaaa atgctgaagt gattgcacgg
    33061 taccaaggtg gcgacaatgc agggcacaca attaagttta acggtgtgac ttataaactg
    33121 catttgattc cttctgggat tttttataaa gacaagatca gtgtgatcgg aaacggcgtt
    33181 gtagtcaatc ctaaatcatt ggttaaagaa ttagcttatt tacatgaaaa aggtgtagaa
    33241 acaagtaatt tgcgtatttc tgaccgcgcg cacgtaatct tgccttatca tatcaaattg
    33301 gaccaattac aagaagatgc taagggcgaa aataagattg gtacaacgat taaaggaatc
    33361 ggacctgcct atatggataa agcagctcgt gtaggtatcc gtattgcaga cttattagat
    33421 aaagaaattt ttgaagaacg tcttcgtttg aatctagatg acaaaaaccg tcaatttgta
    33481 aaaatgtttg attcagaacc acttgatttt gacgatattt ttgaagaata ttatgaatac
    33541 ggacaacaaa tcaaacaata cgtcacagat acttccgtta tcttgaatga tgctttggat
    33601 gcaggcaaac gcgtgttgtt tgaaggcgca caaggtgtaa tgctggatat cgaccaggga
    33661 acttatcctt ttgttacttc ttctaaccca gtagccggtg gggtaacaat cggaagcggt
    33721 gtagggcctt caaaaatcaa taaagttgtc ggcgtctgca aagcctatac gtcacgtgtc
    33781 ggtgatggac catttccaac tgaattattt gatgaaacag gagaacaaat ccgcgaagta
    33841 ggacgagaat acggtacgac aactggtcgt ccgcgtcgtg taggctggtt tgattctgta
    33901 gttatgcgcc attctaaacg agtttcaggt attaccaacc tatctttaaa ctcgatcgat
    33961 gtattaagtg gattggaaac agtaaaaatt tgtacggctt atgaattaga tggcgaacta
    34021 atctaccact atccagcaag cttgaaagag ttgaaccgct gcaaaccaat ctatgaagaa
    34081 ttgccaggct ggtcagaaga cattacagga tgcaaaacac ttgcagaact gccagaaaac
    34141 gcacgtaact atgtacgtcg tatctcagag cttgttggtg tacgtatctc aacattttct
    34201 gtcggacctg atagaacaca aacaaacatc ttagaaagcg tctgggcgca aatctaagga
    34261 taaaaacgac gactgaatca ggacgttctt accagagcga gtcgaagtgt ttatctatac
    34321 gataataata acttgaagag gtagtgaaag agacgtttag ccttattgcc gataggctgt
    34381 ctcttgaaca ctgtttattc agaataaggg actgtgacaa aaac
//
LOCUS       DAZBTF010000024        33096 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000024 DAZBTF010000000
VERSION     DAZBTF010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.024"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..1259)
                     /locus_tag="ACHGNS_001228"
     CDS             complement(27..1259)
                     /locus_tag="ACHGNS_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFN6937188.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1514..2083
                     /locus_tag="ACHGNS_001229"
     CDS             1514..2083
                     /locus_tag="ACHGNS_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN6937189.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2261..2701)
                     /locus_tag="ACHGNS_001230"
     CDS             complement(2261..2701)
                     /locus_tag="ACHGNS_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFN6937190.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2859..3623
                     /gene="map"
                     /locus_tag="ACHGNS_001231"
     CDS             2859..3623
                     /gene="map"
                     /locus_tag="ACHGNS_001231"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFN6937191.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3655..4578
                     /locus_tag="ACHGNS_001232"
     CDS             3655..4578
                     /locus_tag="ACHGNS_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFN6937192.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(4803..5042)
                     /locus_tag="ACHGNS_001233"
     CDS             complement(4803..5042)
                     /locus_tag="ACHGNS_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6937193.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(5047..5466)
                     /locus_tag="ACHGNS_001234"
     CDS             complement(5047..5466)
                     /locus_tag="ACHGNS_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFN6937194.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            complement(5693..6895)
                     /gene="mef(H)"
                     /locus_tag="ACHGNS_001235"
                     /gene_synonym="mefH"
     CDS             complement(5693..6895)
                     /gene="mef(H)"
                     /locus_tag="ACHGNS_001235"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFN6937195.1"
                     /translation="MLVVIGQIISLFGNAILRFALPYYLLKETGSSALFGIATACSFL
                     PMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPIIPLFIVALMLLYG
                     ISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGVVFQFYGINPILI
                     VSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKKEKPIFISVIFLV
                     CIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGILTALVASKLKLK
                     NLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTMFVVQIYTLVQTQ
                     TPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVASIASIMISIYSK
                     RIFQKLEI"
     gene            complement(7037..7672)
                     /locus_tag="ACHGNS_001236"
     CDS             complement(7037..7672)
                     /locus_tag="ACHGNS_001236"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN6937196.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            complement(7786..7878)
                     /locus_tag="ACHGNS_001237"
                     /pseudo
     CDS             complement(7786..7878)
                     /locus_tag="ACHGNS_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(8014..8649)
                     /locus_tag="ACHGNS_001238"
     CDS             complement(8014..8649)
                     /locus_tag="ACHGNS_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN6937197.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            9161..10303
                     /locus_tag="ACHGNS_001239"
     CDS             9161..10303
                     /locus_tag="ACHGNS_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFN6937198.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            10296..10502
                     /locus_tag="ACHGNS_001240"
     CDS             10296..10502
                     /locus_tag="ACHGNS_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFN6937199.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            10581..11774
                     /locus_tag="ACHGNS_001241"
     CDS             10581..11774
                     /locus_tag="ACHGNS_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN6937200.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            11979..13118
                     /locus_tag="ACHGNS_001242"
     CDS             11979..13118
                     /locus_tag="ACHGNS_001242"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFN6937201.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            13111..13911
                     /locus_tag="ACHGNS_001243"
     CDS             13111..13911
                     /locus_tag="ACHGNS_001243"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFN6937202.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            13911..14738
                     /locus_tag="ACHGNS_001244"
     CDS             13911..14738
                     /locus_tag="ACHGNS_001244"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFN6937203.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            14743..15450
                     /locus_tag="ACHGNS_001245"
     CDS             14743..15450
                     /locus_tag="ACHGNS_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN6937204.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            15550..16416
                     /gene="rfbA"
                     /locus_tag="ACHGNS_001246"
     CDS             15550..16416
                     /gene="rfbA"
                     /locus_tag="ACHGNS_001246"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFN6937205.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            16430..17002
                     /gene="rfbC"
                     /locus_tag="ACHGNS_001247"
     CDS             16430..17002
                     /gene="rfbC"
                     /locus_tag="ACHGNS_001247"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFN6937206.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            17024..18052
                     /gene="rfbB"
                     /locus_tag="ACHGNS_001248"
     CDS             17024..18052
                     /gene="rfbB"
                     /locus_tag="ACHGNS_001248"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFN6937207.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            18150..19001
                     /gene="rfbD"
                     /locus_tag="ACHGNS_001249"
     CDS             18150..19001
                     /gene="rfbD"
                     /locus_tag="ACHGNS_001249"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFN6937208.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            19035..21068
                     /locus_tag="ACHGNS_001250"
     CDS             19035..21068
                     /locus_tag="ACHGNS_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937209.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            21112..22392
                     /locus_tag="ACHGNS_001251"
     CDS             21112..22392
                     /locus_tag="ACHGNS_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFN6937210.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            22602..23408
                     /locus_tag="ACHGNS_001252"
     CDS             22602..23408
                     /locus_tag="ACHGNS_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6937211.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            23420..24640
                     /locus_tag="ACHGNS_001253"
     CDS             23420..24640
                     /locus_tag="ACHGNS_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6937212.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            24630..26216
                     /locus_tag="ACHGNS_001254"
     CDS             24630..26216
                     /locus_tag="ACHGNS_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN6937213.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            26255..28393
                     /locus_tag="ACHGNS_001255"
     CDS             26255..28393
                     /locus_tag="ACHGNS_001255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN6937214.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(28761..29744)
                     /locus_tag="ACHGNS_001256"
     CDS             complement(28761..29744)
                     /locus_tag="ACHGNS_001256"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFN6937215.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            30122..31513
                     /locus_tag="ACHGNS_001257"
     CDS             30122..31513
                     /locus_tag="ACHGNS_001257"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFN6937216.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            31529..32569
                     /locus_tag="ACHGNS_001258"
     CDS             31529..32569
                     /locus_tag="ACHGNS_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN6937217.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            32588..>33096
                     /locus_tag="ACHGNS_001259"
     CDS             32588..>33096
                     /locus_tag="ACHGNS_001259"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFN6937218.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNG"
BASE COUNT        10660 a         5738 c         6537 g        10161 t
ORIGIN      
        1 ttttttgcat gtgtgcgaaa gtatactcaa gcccaatcgc catttcggaa aatcggtact
       61 gtagagccat cttcacggat tccgtcgata tccatcttgt cagatcctac catgaaatct
      121 acgtgggttt gacttctatt caatccagct tctgctaatt cttcatcgga catttctgta
      181 ccgcctttca cactgaaggc ataagcagaa cctagtgcta agtgattcga tgcgttttca
      241 tcaaataatg tattgaaaaa gatgatgcca gattgagaaa taggtgaagg gtcaggtaca
      301 agtgcgactt ctcctaaacg acgcgcacct tcatctgtat caagcagttt tgctaagaca
      361 tcttctcctt gttcggcaga aaaatcaaca acttttccat ctttaaaagt aaatttcata
      421 cccgagatga tggtgccagc ataactcagt ggctttgtac ttgaaatgta accatctact
      481 cgatgactgt caggtgctgt gaacacttct tccgtcggca tatttgccat gaatttttct
      541 ccacgagcat tatagcttcc agcgccttcc caaagatggt ttttaggcaa tccgataatg
      601 atatctgtac cgggagcagt atagtgaagg gcagaaaatt gttctcgatt caattcttct
      661 gctttttttg ctaatttttc atcatgcttc ttccatgcta agacagggtc ttcttcatag
      721 acacgagtcg ttttgaaaat ctgatcccat aatgcctcaa cttgtttttc ttctggcagt
      781 tctgggaaga cttttgctgc ccattgttta ccagccgcag ccacgacagt ccaactgact
      841 ttatttgctt gagttgcctt gcgtaaattc attagtgctt tgccattcgc tgattgataa
      901 gacgctactc gatggctatc tacaccagca aatgcatcgg gatcggcaga aacaacgctg
      961 atcctgcttg ctcctttttc aagccattca tccgcttgat cgattttaga ttgtggtaca
     1021 ttctcaatac gatccgttgc agcatgtaaa agaaattctc tttggatctg gtcgtcagtc
     1081 cattgaacga taacctcagc ggctcctagt ttataagctt cttgtgtgat caatcgagca
     1141 aggggtgcct gttccacgct gatctgtaaa acaactgtgt gtcctttttc tgtggctacg
     1201 ccagtttctg caattaagcg tgcatatttt tttagaagtt cattaaaatc agataacata
     1261 ataaaaattc ctcctcacca ttttttctgg taactattag catgatacca tttaattatg
     1321 attcaaacaa tgaggcttta aataaaaaaa ggaggttatt tttttaaggt taagtttcat
     1381 gatatatgtt actacttgct atttcagctt gtgaagaaaa tcaaaaacta attttgcata
     1441 tcaaaaaaaa catgttacaa tgaaattaat caatggtgga agcgttataa aaaaattaag
     1501 gagattaaaa aatatggcaa gaaaaaagac gattactaga gaacagattt tggcagctgc
     1561 ttatgaagta gctgccaccg atggatttac acgatttact gcgagaaaca tcgcgaacaa
     1621 aatgaattgt tccacgcaac cgatttatct tgagtttaaa aatatggatg acttgagaca
     1681 agctcttttt gagcagatct atgatcattt acaatatgaa gtgttcactg ttgagcatac
     1741 agggaatacg attatcgatc ttgcttttaa ttatatccat tttgcaagaa gagagaagaa
     1801 gttgtatcgt tcactgtact tagaagaata tggtggagga aaagagatgc agaatttctc
     1861 ttatacttac tttgtgaata cagtgaaacg cgatccagct tatgtgaatc tttcagaaga
     1921 gcagatcgaa tctttgtata atgggacatg gatcatagct actgggatcg cagctttgat
     1981 gtcttcaagc atcatccatc caactgatga acagattgaa aagatgatcc aagacagtat
     2041 agatgctatc ttgaaattca aagaaccaat cgaaatcgat tgaatatact caaaagttcc
     2101 acaaatcagt aggtaacaag agatgtgtca aaataaggaa aagttcgttt ttcttacagc
     2161 gtatgcattt ttaactcccg aagaactctt ccatgaatgc aatgaataaa agagccatac
     2221 agattgaata aatctggatg gctcttttat tgcaagtata ttaaattttt gcgactagtt
     2281 ctttagcgaa agcttctaaa ttttcgatat cttcttcttc agcattcaga tccactttaa
     2341 cattttcggc tccttttgat gcgcctgttt ttgcaaaagc agcatcgaaa tcatccaccg
     2401 atttgcagaa ttcatcgtaa aaagtatcac cagatccgca gacaccgtaa atttttccca
     2461 atagatccaa ttcttgcaag tcttcataaa agtctacgat ctcatcaggc agatcgccat
     2521 catcgtatgt ataagtagct actacgcaaa tatctgcctc ctcgaattca tctgcatcta
     2581 cttgggtaca ttcgttgatt tcgacttcga tatccagatt ttcaaatgct tctgcaacaa
     2641 tatcggcgat ttcttcagtg tttcctgtca tgctagcata tacgattttt gctaaagcca
     2701 tgatgtgcct cctcgtttat aattatttaa caattatgaa ttataccaaa acatcattga
     2761 aaagaaaata aaacctagag acacctgggg aatgtcattt ggctaaatgt atgccgctat
     2821 gttaaaatga gagggatttg taaaaaggag acgaaaatat gattacttta aaatcagcac
     2881 gtgaaattga aatgatggca gaatctggag ctttattagc agatgtacat aaaaatctgc
     2941 gtgattttat aaaaccagga atcacaagtt gggatattga ggtatttgtc agaaactata
     3001 tcgagagtca tggcggaatt gctgcgcaaa ttggctttga agggtatgaa tatgcgactt
     3061 gtatcagtat aaatgatgaa atttgtcatg gattccctcg taaaaagcct ttgaaaaacg
     3121 gtgatttggt aaaagtcgat atgtgtatcg atttgaaagg cggcatttct gattcttgtt
     3181 gggcctatgt agttggagaa tctacacctg agatcgaccg attgatggaa gtaacgaaga
     3241 aagctttgta catcggtatt gagcaagcac aagtagggaa tcggattggt gacatcggtc
     3301 atgctatcca aacttatgta gaaggcgaaa atctagcgat cgttcgtgat tttattggtc
     3361 atggcgttgg tcctacgatt catgaagaac cagcagttcc tcattacgga gaagctggaa
     3421 aagggttgcg gttgaaagaa ggcatggtta tcacgatcga acctatggtt aataccggaa
     3481 cttggaaaat gaaaatggat cctaacggct ggactgcgta tacaagagac ggcggacttt
     3541 cttgccaata tgaacataca ttggctatta ctaaagatgg tccaagaatt ttgacatcac
     3601 aaggagaaga aggcacttat tgatctatcc agtcaatagg tacgggagag gtacatgaaa
     3661 gtttggaaca aaataaaaca aaatgaaaat ctgatgcgct ttattgagac gactcaaaag
     3721 cgcatcactg attcagaaat gagtacgacc tctgtcgtcg ttgcttatta tttactcttg
     3781 tctttatttc cattgattat tgcttttggt aatattttac cgtttctcca catcgatcaa
     3841 gagactgtac tgacctattt acgagaagtg attcctgaga ccgtttacca gtttatcggt
     3901 cctgcaatca aagacctttt gacacaaagt tcaggaggct tgttatccgt ttctgcatta
     3961 gcggctttat ggtctgcaag tcaaagtatc aatgctttgc aaattgcgat gaataaagcg
     4021 tatggcgtag aaaatagaaa aaattttttg atcgtccgtt tcttctcact aattgtcatt
     4081 gtcttgttca tgattgctat tagtggggta actatcgttc ttgggttggg acagctgatt
     4141 ttggatgcta ttcagccaat cgtacaaata cccgtgaact ttatcgatca gttccaaact
     4201 ttgaagtggc caatcacatt ggtagcttta tttgtcatca tgttcttgat ttatttaatt
     4261 gtccctaatg ctcagttgaa gttgaagacg atcattccgg gcacgatctt cgctactgtc
     4321 ggctggatgc tgttatcaca agtgtttagt atctatgcta ggtattttgc aacgaggatc
     4381 agcggttatc agatcatagg aagttttatt gtactcatgc tttggcttaa ttttgctgct
     4441 acgatcatca ttttgggcgg aatcatcaat gcagttgttc aagaatacgt gacgggtcat
     4501 caaatcaaaa aacgtcgaga tccaagccga aaatggttca ataaattgaa aactaaattt
     4561 tctcataaaa aaaagtagaa acaagagttc ttattgtaaa tttatatcag taatttattt
     4621 gaaattttat atctaatttt caattatccg cataaacact atacattcaa gcattttgaa
     4681 gtatagaaaa acactcatct taccacgaat ttaccacgat tgaatgagcc ttgatatgac
     4741 cgtaaaacta ctaagggaga tagcacgaaa ctgtgtatct cccgattttt ttatacagtt
     4801 gattacttca ctccgatttc aaatttcaat attgctttca tcatgtctgt atgaatttct
     4861 ccttttagtt cttcatcaac ctgcataatg attttaccac tttcagagtg gtaaaaagga
     4921 cgcaagcata atttagaaat ataaccgtca taaaacttca aaatctcttt aactgccatt
     4981 tcatctccgc ttgacgcttt gcaaatcaat tcaaaagagg gatagccata atctttactc
     5041 atttctttac ttcctttcgc tcaaaagcat ttttaacttc cttagtcctc tagttcttct
     5101 actgcaaata gcagaacgtg atacatggta cagttctgaa atttctctgt cgttcattcc
     5161 ttgaaaataa cgtaataaaa tcgcactacg ctgtttttct gacagtctat gtaaaactgt
     5221 accaagtgtt tcatcagata cataaattgt aaagtgcaag actttaaatg atacacaatc
     5281 ataaaaacag gtatcaatcg ttccacgttc aacttgtttc gtttcggcta tttcacaaaa
     5341 caatacttca cgtttagaat gtcttgcaag ctgtctgttt cgacttttca ctttgttctt
     5401 aatgacaatc agcattaaag aattgaactg taatctgacg atatgctcga aagaagatgt
     5461 cctcataatc tcaccccctt tctgctaatg ctgaaatata taaacggaat accgtttata
     5521 ccctttagaa atacagaaaa atggtaagaa aaaagcccac acaaaacata tatttgtgcg
     5581 agccttaaat actgcaatat tactctttct tttataacac tatcctttta gcagatttca
     5641 aattttcttt tatatattgt ttttagccta acatactatt taaccccttg tattatatct
     5701 ctaacttttg aaaaattctt tttgaatata ttgaaatcat gattgaagca atagaagcta
     5761 caaacataac aacccatgtt tcttgtataa atacatcgaa taaaataccg taaaccattt
     5821 gtccaatagg ttgaccgcac atagcaactg ctaccagcgt agccattatc tttcctacta
     5881 attgcggtgg tgtttgtgtc tgaacaagag tgtaaatttg aacaacaaac atagtagata
     5941 cacccatagc cacaaagcac atcactgtaa ctacccagta ggaaactatt gcaggcaatt
     6001 ctaaaaatag cgaaattccc ataagtaaaa cagaaacaga acaaataagt aacaaggtat
     6061 aaaggttttt tagttttaac tttgaagcaa caagagctgt taaaatacca ccacataaac
     6121 cacctaatga taatgctgat tgtgaaaaac catatagtgt atctggcatt tttaaaactt
     6181 cagaaattaa gataggcaga ccaacaatta aaacagcact taaaaccaaa ttgaaaatgc
     6241 aaaccaaaaa gattacggat ataaaaatgg gtttctcctt tttcaaaaag gtataactat
     6301 cttttaaatc gtttttagca actgcaatta cacctacttc acgaggtctt ttttggtgtg
     6361 gaatatggat aaaaatttcc ataatggctg aaaaagtaaa acatataata ctaacaatta
     6421 aaatgggatt gataccataa aattgaaaaa ctactccgct tatagctggt ccgataaagt
     6481 ttgctaaagt atttacttga tttattatag cattaccgac ggtaagttta tctgatgata
     6541 ccaatagagg aatacttgct tgtaccgctg gttgatatgc accagaaata ccataaagta
     6601 acatcaaagc aacaataaac aaaggtatga taggaatttt tcccaatgac aaataaaata
     6661 ctaaaattat gattgctgtt acaaaatcta gtatgaccat aatgtttctt ttatttgtcc
     6721 tgtcagcaat aataccacct accagcgaca aaataaccat aggtaaaaaa gagcaggctg
     6781 tagcaatacc aaacaaagct gatgaaccag tttcttttaa caaatagtac ggtaatgcaa
     6841 atcgtaggat agcgtttcca aataaagaaa ttatctgtcc tataacaaca agtataaaat
     6901 cacgattaaa aagttttttt ctgcttgcat aaaatctctc ctttttattt aatcaaacag
     6961 accgtcggtt tatataatgg tttaaataaa ctgctctttt atagagcagt ctatcgtata
     7021 gggataacaa atacagttat ttacaaaact gtataaatgc agaaattgtt tcttcgtcta
     7081 ataatgtgtc tatgcctatt ccatttaaaa gggcattgaa tgtattgcaa cgtttttcca
     7141 taccctcttt atccgtcatt tcaactaatg ggttgagcca tacattactt aataccatga
     7201 taacttctgc aatttcatgt gaattttcaa tctccaaaga cttatccttg ataccttgtt
     7261 ctaaaacggg ttcaacaaat tcaggtacta caatttcata catttgttct atttgcatag
     7321 caagaaaacg aggattgttt aatagttgag gagaaactgt caataaaacg ctttggttag
     7381 aattgaatac cgctgtctta aatatttctc ttaacttttc taaaccgttt aaactatcgt
     7441 tatctctaat tttagcaaat attactgcat tttcatttcc aattctttga aaaattgctt
     7501 ttaaaatatc ttcctttgaa tcgaaatgat gataaatagc acctttagaa agttttgttt
     7561 cattgataat atcttgcaat gatgtgttat catatccttt ttcacaaaac atttttgtag
     7621 aaacatctaa aatgagtttt actgtttctt ctggatattt atttcgtgcc ataacttacc
     7681 tccaatacaa accgttggtc tgattaaagt atatcttagt attttatgct tgtcaatata
     7741 aagacttgca attttataaa aatgcagaat gcaagccttt ttcatttatt ctacaatctt
     7801 ccagttgctt tccttatgcg gtgtcaattc atactgtgat acctgcgttg cctttgcctg
     7861 atttaccaca aatcagattt tatccctttc ttcctcttga ttgcccacaa cagaaaagaa
     7921 aaagccctgt caagagcttt gccaccattg gtggtgcttt gcactcttga tcgggctttt
     7981 tgtttgctgt atcttctcgc aagaggaaaa aagttactcg tcctcatcat caaaattgtt
     8041 attccttaat acttctcgta acagttccat atcttctttt gagttcgggt ttatcatttt
     8101 cacgacttga taaggtgtct tatacttatg tccctctatg ctttccccaa acatatccat
     8161 actgtataaa tcatcatagc ggtctaataa tttttgaaac ttcaaatgct ggataaattc
     8221 tttgattggg taaaggtcgg acgcttcaat gcttttcccg ttgatatagt ctgtaatata
     8281 ttcccgtaac ttttctaact catattccag ccattcttcc tcgctgtcaa agaagttatc
     8341 atagtgtcca tgtttcagtt ctgcatttag acgttcttcg gctcttaaaa actgacagac
     8401 ttccttttca gattgattga catacttcca ttctcccgac aacaattcat tgggcgtaac
     8461 gtccagcact tccattatct tttccagcgt ttcataggca ggatagttca accctctttc
     8521 aatcttggaa aggctctgca tattgatacc gattttgtcc gcaagttcct gttgtttcat
     8581 tcccctaaat tttcttatgg tctgtatatt atttcctaaa tggcttattt tcattgcata
     8641 accctccata tctcgttata tcttcgctat tataagtcta tcatgcttaa tatgatttgt
     8701 aaaggcaaaa aaagatattg acaaataagc atataagaat tactatgttc ttgtaatcga
     8761 ataagcacaa tagaatgaaa tataaaaaca ttcaaaaaga aatacctatc tgattaaatc
     8821 cgtagatatg aatatcggtt gaaatggaac ttgttagaaa aggcaaaaca gaacttgctg
     8881 acaaagtatt tctgacacgg tggcggaagc ctctcaggct taccgccatg acaccttgtg
     8941 tcaaggggaa gaaatgcttt gtcggctggc gagcctgcga gccttgtctg tgcccccgtt
     9001 atctaacagg ggggcactat ttcacaaaag acaagtcaaa aaccatacac ccccaaacct
     9061 gtgagtagta atgagaggtt ttaagaagat agtacatatc acactttttg aaaggtggtt
     9121 tacaggtatt tcaaggctat tgtgggattg gaggaataca atgaacaatt tagaatttgc
     9181 acttgaaatg aagaacaaac gtcttgccag cggtctttca caaggagaac ttgcaagtct
     9241 tacccatgta tcaagatata gtatcaaccg ctttgagaac ggaaaagcta acgccagcaa
     9301 ggaaacacag aacattatcc tgcgttgtct gaattactat atctgcgata agccttttta
     9361 tctgctcgtt gattatctgt ctgtccgttt tccaacgact gacgcattgg aagttatccg
     9421 aaaggtgctt ggcatgaaag cagattattt tatccattat gactatgggt attacggcta
     9481 taaggagcat tatgcttacg gggaaatcaa ggtcatggct tctgatgatg aacacatggg
     9541 cgtattttta gaattaaaag gggcaggctc acgcaacatg gaatatgtct tacaggcaca
     9601 gaacagggac tggtattcat tcttaaaccg ctgtcttgac tgtggcggtg ttatccgacg
     9661 ttttgacttg gcaatcaatg atatgtgcgg tctgctggat attcctgtgc tatctgaaaa
     9721 atacagaaat ggtggtgcgg aatgtcgctg taaaaactat gaaaatgtac aaggtggaaa
     9781 attaagcgga aaaaaccgta atttagcaag taccctttat atcggctcaa aggcaagcac
     9841 aaaatatttc tgcctgtatg aaaaacaaaa ggaacaggca acgaaaaaga aacataccga
     9901 tattatcaac cgttttgaaa ttcgtctgcg tgacaaaaag gcggtacagg cagttgaaga
     9961 actgttattg acgtataacc ctcatgggct ggtgttttat ctcattaccg attttgtgga
    10021 atttcctgac tatccattgt gggagatatt catttcccat gacagtttac cttttgaaat
    10081 gaaccctgta cctgtcaata tggaacgcac tctgcaatgg ctggaaagac aggtcatgcc
    10141 gtcaatcgtg atgatagagg aaatcgacag gctgacaggt tcaaactaca tgaaaatgat
    10201 tgatgaatgt acccaccttt ctgaaaaaca ggaaatgctt gtggaacaga tgtgtacgga
    10261 catatcagac gtaatcgaaa gtgagggggt gttttatgag taatacacag gatattcccg
    10321 tatgggaaaa atacaccctt accattgaag aagcgtcaaa gtatttccgc attggagaaa
    10381 acaagttaag acgattggca gaggaaaaca aggacgctgg ctggctcatt atgaatggca
    10441 accgcataca gattaaacgc cgacagtttg aaaaggttat tgataaattg gacgcaatct
    10501 aatgcaaatg agccttgtat gtgttatgat gaacacaagt catatcaagg ctctttccaa
    10561 caaggaaagg agcagacacc atgaaagaaa aaagacggga taacaaaggt cgtatcctgc
    10621 atactggaga gagccaacga acagacggaa aatacttata taaatatgta gacgcattgg
    10681 gaaacacaaa atatgtgtat gcttggagat tgacacccac agacccgaca ccaaagggaa
    10741 aacgggaaaa accctcactt cgagaactgg aacagcagat aagacgggat attgaggacg
    10801 gtatcgacag cacaggcaag aaaatgacgc tttgccaact ctatgccaaa cagaacgcac
    10861 agaggacaaa tgtgaagaaa agcacacaga aacaacggga acaactcatg cggttattaa
    10921 aagaggacaa gttaggtgct aggagcattg atacgataaa accctctgac gctaaagaat
    10981 gggcgttacg catgaaagac aaaggctttt cctacaatac cattaacaac cataaacgct
    11041 cgttaaaagc gtcattctac atagccatac aagatgatta tgtgaggaaa aacccttttg
    11101 atttcaagct aagtgaagtc atagaaaatg ataccaaaga gaaagtcgca ttgacagagg
    11161 aacaggagca agccttattg tcatttatta agacggacaa cgtgtatcac aagcattatg
    11221 atgatgtgct gatactgtta aagacaggac ttcgtatctc ggaactgtgc ggactgacag
    11281 tagccgacat tgatttcaag aatgaagttg tgattatcga ccaccagtta ctaaagagta
    11341 aggaacaggg ctattatatt gaaacgccta agacgaaaag cgggataagg caagtgccat
    11401 taagcaaaga aacgatacag gcatttcaac gggttatcaa cagccgacca aagataaagt
    11461 cgatagaggt agacggacac agcggttttg tgtttatcaa tccgaaaggt aatccaaaag
    11521 ttgccattga ttataacgcc ttatttatcc gtatggtaaa gaaatacaac aagcaccaca
    11581 aggacaatcc cttgccacat attacaccgc atacgctacg ccatacattc tgcacaaggc
    11641 tggcaagcaa gaacatgaac ccgaaagatt tacagtatat catggggcat tcaaacatca
    11701 gtatcacaat gaactggtac gctcatgcgt ccatagatac cgcaaaatca gaggttcagc
    11761 gtctaatcgc atagaagtat ttaccacgat tttaaccacg attgacaggg aaaatataag
    11821 aagatagacc tagatatgcg aggtttacca caaaagcaaa atgcccgtag agccgataaa
    11881 ataaggcttt gcggacattt gagaagatat aaaaagatag tcaaaaagac atatataatt
    11941 tataataatt atgactctta attaaagtag gtgcccatat gcttgtttct tttagtatgg
    12001 ttataagatt ttttctgaca attttattat cactgatatt aacacctgta ttcaaaatca
    12061 tttcagtaca aacaggtatg gtcgacaaac caaatgaaag aagaataaat aaagttccaa
    12121 tgccttcagc gggcggactg cctatcttta tttcatttgt tatatcgact ttattcttat
    12181 tccgtaaaat tatccctcaa tcgtatattg taccgatctt gattgcttct ttagtaatca
    12241 tagtcacagg agtgctcgat gataaatatg agctgacacc taaacaaaaa agtataggga
    12301 tcttgattgc ttctcttatt atttattttg tagcagatat acgtattgat tccttcacgc
    12361 ttccttttat tggatatatc caactaggct ggcttagttt tcccgtaacg attttttgga
    12421 tcttcggtat cacgaatgcg gtcaatctga ttgacggttt ggatgggctg gcggcaggga
    12481 tttcattgat cggtttaatt acgataggaa tcattggata ttttttcctc catgcttcta
    12541 cagtgtatat tccagttgtt attttctgtc ttgtagcaag tattatcggt ttttttcctt
    12601 acaattttta tccggcaaag atttatttag gcgatacagg agctctattc ctagggttta
    12661 tgatggccgt cctttcacta caagggctga agaacgtgac ttttgtttca tcgatttctt
    12721 tgctgatcgt catgggagtg ccggtaacag acacgttttt tgctatcatt aggagaaaag
    12781 caaaccgtgt ttctttttct acagcagata aaaaacatct tcatcaccga ctaatcagtt
    12841 taggctttac tcataaaggt gctgtcctga cgatttatgc gatggctttg atgttttctt
    12901 tcacagctat ggtcatgaat tatactggaa gaatcggaac gatcatattg attattgcca
    12961 tgttgtttgc tgcaatttta ttatttgaat tgattggtct gatcaatgag aaacatcagt
    13021 ttattttaaa aacattgcga tttttaggga ataaagagta ccgtacgcaa gtaaaacaga
    13081 aatatttgaa gaaatggaac cgtcatatag atgaataaca aattaaacca gccgatcctt
    13141 attagtattg tgacggcaaa cagtgataaa atttttcgga cactagataa ttttatttca
    13201 actgtaaaag atgaaacagc agtaaaaatc cgaatttttg acaacaattc acttcccgag
    13261 tacaaaaaaa gactaacaga gtataggaaa tatccctaca tcgatatcta ttttcatgat
    13321 gaaaattccg gatttggtta tggacataat cacaatctga tagctagcga ttctcgctat
    13381 ggcattattt gtaacccaga tattttagtg gatgaagaga cagttacagc attggtcttg
    13441 ttattgaaag aacatccgga atgtgcgatg ttagcaccta agattcttaa tgaagatgga
    13501 acaactcagc acctcatccg tgagcagctg gctgtttttg attacgtttt gcgctttgtg
    13561 cctttcaaat gggtcaaaaa aaagtttgac aaacgactgg ctgtgtttga atgccgagga
    13621 ttgcctactg atgctaatag ttatgtacgg atgatctcag gtagttttat ggtagctgat
    13681 attgataaat ttaaagagat aaaggggttt gatgagcgtt tcttcatgta ctttgaagac
    13741 aacgacttgt gtttgagttt tgaaaaagca ggttacaagt tattgtacac acccttttat
    13801 gcagttatcc atttttacgg gaagggtgcg catcgaaatg caaaactgtt tcgcgtgttt
    13861 ttgacttcta tgatgaaatt tttcaataag tgggggtggc gttttttctg atgaaaccag
    13921 aaatattagt agtcgttgtg ctttatcagc aactattcag tcaatctcca gcttatggac
    13981 cattaagcaa agcacttagc gaaaagcaaa ttcagcttat tatttacgat aatagtcctc
    14041 aaaggcagca aaatgagctg tttgaaaaag aaaatatcgt ttatcatcat gatccgcaaa
    14101 atcctggttt agcggtggct tataattttg cgttgtctca agcagatgaa ggaacacgtt
    14161 gtctcgtcac tctggatcag gatacgaggc tagtcgacga ttattttgaa ctattgcgta
    14221 aagtaacctt tacagatgaa tgtgttgctg cagttccgat gattttttct gggagccgac
    14281 aagtttctcc cctatattca gatcgttata tcaatcgtac agctcaagca gtagaagttg
    14341 aaggcccaac agctgaacga atcatggcaa tcaattcagg tgttgcctgg tctgtcaaat
    14401 ttctaaaaaa gattggcggg tttaatcctg ctttttcttt agattttttg gatcattggc
    14461 tgttttggaa agtggatcag ttggaaaaaa ctgtggagat ccttcctgta cgaatggaac
    14521 atgatctttc ggttttagac tatgaaaaag tttcaagtaa tcgataccgt tctattttac
    14581 aagcagaaaa tcgattttat catgaatatg atcaaaggca tttatccagt caccgacgac
    14641 aattattgtt aagaacagct aaacagttct tgaccgtcaa aaaccggcat atctggcgga
    14701 tgactttgcg ctcatatgtt aagaattgga aggtgtgaat tcgtgcgttc ggtttgtata
    14761 gctaccttta atggaggcag atatttaaga gagcagttag atagtatttt atctcagctt
    14821 tcagcagaag atgaagtagt catttctgat gatgggtcag tagatgatac atgggatata
    14881 ttaactgaat acgcagaaaa agataagcga atccagcttt tttcggggcc tcgtcaagga
    14941 ttgatcgcta attttggtta tgcgatccag cagacgaaag gagatctaat ctttttggca
    15001 gaccaagatg atgtttggtt ggagacaaaa gtgaaaaaga tcactgacta ttttgagcag
    15061 aatccggatc agctagtagt agtttctgat ttaatcattg tggacagcga gctgcaagag
    15121 atccatgatt cgtatttttc gtttcgaaat tccaaagaag gatggcttaa taatgtgata
    15181 agaagtcact atattggtgc tggtatggct ttccggtctt ctttaaaacc cgatattttg
    15241 ccgatcccgt cagaagtgcc gatgcatgat atgtggattg gtttgctggg aggaaagaag
    15301 actgggttcc taagagaacc gttaactctg taccgaagac atgatttaaa cgcgagtgag
    15361 attgaaacac attctaaatt acggcaaaaa atttcttggc gtgttcatct gattacggcg
    15421 ttgatcaaac gaaaatggtt taaacgttga taaaagatgt ttaaatcctt aatatttcca
    15481 tgacaaatat aacaattgat gagataataa caaagtgaaa tttgaaatca aaaaggagag
    15541 cagatatcca tgaagggaat cattcttgct gggggcagcg gaacacgtct gtacccttta
    15601 acaaaggcaa catcgaaaca attgatgccg atttatgaca aaccaatgat ttattatcca
    15661 atgtctactc tgatgttggc gggaatcaaa gaaattctga ttatttcaac gccacaagat
    15721 acaccacgtt tcaaggaatt atttggaaac ggaaatgatt taggactgca gatcgaatat
    15781 gcagtacaag aaagtccaga tggattggca caagcgttta tcattggaga agaatttatt
    15841 ggagatgata gtgtctgctt ggtattaggg gacaatattt actatggtgg cggtctgtct
    15901 aaaatgttgc aacgagcagc ttcaaaagat acaggcgcga cagtatttgg ctatcatgtc
    15961 aatgatccag aacgctttgg tgtagtagaa tttgatgaga atatgcaagc tttgtcgatt
    16021 gaagaaaagc ctgctcagcc aaaatcaaat tacgctgtaa ctggccttta tttttatgac
    16081 aatgaggtaa tttctatcgc taaaggtatc aaaccttcag aacgtggaga actagagatt
    16141 acggatgtca ataaagcata tttggataaa ggaaaactat ccgtagaact tatgggacgc
    16201 ggatttgctt ggcttgatac aggaacacat gaatcattgc ttgaagcatc tacctttatc
    16261 gaaacaattg aaaaacgtca gaacttaaaa gtggcttgtt tggaagaaat tgcttaccgt
    16321 atgggctaca tcgataagga acaattgctg gcccttgcac agccattgaa aaaaaatcaa
    16381 tacggtcaat atttattgaa tttggccgct gaataaggag tccttacaga tgaaagtaac
    16441 agatacaaaa ttaacagatg tcaaaatcgt agaaatggac gtctttggcg atcatcgcgg
    16501 tttcttcaca gaaagttatt caaaagcaaa atttgcagaa catggattga aatatgactt
    16561 tgttcaagac aaccattcac tatcagctga agctggtgtc cttcgggggc ttcatttcca
    16621 aaaaggagaa gctgcacaga caaaattgat tcgggtagtc actggagcag ttttagatgt
    16681 agtagtggat atccgcaaag gtagcccgac ttatggtcaa tgggacggat ttatcttatc
    16741 tgaacataat catcgacagt tgcttgtacc taaaggatat gcccatggat tcgttacttt
    16801 gacaccaaat gtgaacttcc tttataagtg tgataattac tataatgcag aagcagatgg
    16861 gggcatcgca tttgatgatc cagatttagc gattgattgg ccaattgatc catcaagagc
    16921 gattatgtcg gaaaaagatc accgtcatcc gacattaaaa gaatttgaag cagaaaatcc
    16981 atttgtttac ggagaaatct aagaattaag gagaaatttt cttatgaaaa acatcattgt
    17041 taccggaggt gccggattca tcggctctaa tttcgttcat tacgtggtaa acaatcatcc
    17101 agaagtgcat gtcactgttt tggataaact gacttatgca ggaaacaaag agaacttggc
    17161 tggacttcct agcgatcgcg tagaattggt cgtaggagat gttgcagatg cagaattagt
    17221 ggaccgtcta gtaaaagaag cggacgcagt tgttcattac gcggcagaat cccataacga
    17281 taattcatta aaagatcctt tcccatttgt acaaacgaat atcattggta cgtatacact
    17341 tttagaagct tgccgcaagt atgatgtacg ctatcatcac gtatctactg acgaggttta
    17401 cggcgatctt ccattacgtg aagacctgcc aggacatggg gaaggcgaag gagaaaaatt
    17461 cactgcggaa acaccatata atccttcaag tccatattct tctacaaaag ctggttcaga
    17521 tctgttagta aaagcatggg tgcgttcttt tggcttacgt gcaaccattt caaactgttc
    17581 aaacaactat ggtccttatc agcatattga aaaatttatt cctcgccaag taacgaatat
    17641 cttaagcggt attcgtccaa aattatatgg cgaaggaaag aatgttcgtg actggatcca
    17701 tacgaatgat cattcttcag cagtgtggct gattttgacg aaaggcagaa ttggcgaaac
    17761 ctatctgatc ggtgcagacg gtgaagaaga taataagacc gtgatggaat tgatcttaga
    17821 aatgatgggt caaccaaaag atgcgtacga ccatgtaaac gaccgtgcag gacacgactt
    17881 gcgttatgca atcgattcga caaaattacg ggaagagtta ggatggaagc ctgagtttac
    17941 gaacttccgt gatgggttgg ctgacacgat caaatggtac gaagaacatg aagactggtg
    18001 gaaaaaagaa aaagaggccg ttgaagcagc atatgccaaa aatggacaat aatacagaga
    18061 aaatgtcact gtattaatgg aaaaacacat gtgaggcagg cgctcgcctg tctctttttt
    18121 aaaataaaaa tcaaggagcg gataatcata tgtttttatt aactggagga aatgggcaac
    18181 taggtacaga attgcgccat ttattagatg aaaaaaatct gcaatatgtt tcaacagatg
    18241 caaaagactt ggatatcaca gatgccgaga aaacaatggc ttacatcaca gaattaaaac
    18301 ctgatgttat tttccattgt gcagcttaca cagcagtaga taaagcagaa gaagaagcaa
    18361 aagaattaga tgagaagatc aatgttgatg gtacaagaaa tgtagcactt gcagcaaaag
    18421 ctgtgaatgc tcaattcgtc tatatcagta cagactatgt tttcgatgga aaaaacaaaa
    18481 atgtagaata cgaagtagac gaccaaacga atccactaaa tgaatatggt cgtacgaaat
    18541 tattaggcga aaaagcagta gaagagattt tagaagacta ttatatcata cgaacttctt
    18601 gggttttcgg gatctatgga cacaatttcg tcttcacgat gcaacgccta gcggaaaccc
    18661 gcgacaaact gactgtagta aatgaccagt ttgggcgacc aacttggaca cgtactttgg
    18721 cagaatttat ggtatatgtc atcgaagaaa aagcaccatt tggtatttat catttgtcga
    18781 acgaaaatag ctgttcttgg tatgaattcg caaaagaaat attgaaagat acagatgtag
    18841 aagttgcacc tgttacgtca gaagaatttc cgcaaaaagc aacacgtccg cagtattcag
    18901 tcatgagcct gaaaaagaca gaagagttag gatttgtcat accaacttgg caagaagcac
    18961 ttgcgcaaat gctggaagct gccaaaaaaa tgaataagta atttcctttt atcaaataga
    19021 atttggagca tgatatggaa aaacaaacaa gaagtgcacg acgaaaagaa aagccaaaag
    19081 cgaaccaggc agattcgaat ttggtgcttg acctgtttct aaaactccgc tatcttattg
    19141 gcgttttagt catcgtggtc atcgttactt ttaatttaaa cggaagttca cttggtgttt
    19201 gggataaata tgtgtcgcaa agagacgatg ggaaaaaaac tgatgtgatt tttggacaga
    19261 atcgggaagt gcggtcagac gaatggctgg tgcagacacc tttttacctc tcacaagctg
    19321 aagaggactt tccgttggtg aacgaggatt attcattaaa cggacaaaac atgatcattg
    19381 cgtataattc tcctgtaaag gatattacgg ttatcggaaa accattcaac tggggattct
    19441 ttttcctcgg aagagatcga ggactttcct tttattgggc gatgaaatta gtcgtcatgg
    19501 tcttgcttgg attcgaagta ctgatgatat taacgaagag aaaaaaagct ctatcgctaa
    19561 tcggtgcatt tgcgctgaca ttttctccag cagtccagtg gtggttcatg caacatgtgg
    19621 gcgatttgat tttctttact ttgggactga tgactgcttt ttatcattat ttctacaatc
    19681 atgataaaaa atggctacga acgttgatga tgcttttggt ggttattttc ggtattgggt
    19741 ttattttagt gatttaccca gcccatcaag tgatgctggc gtacttgctg attttttatt
    19801 ttgttggcct gcttatttac tatagcaaga aagtttcatg ggatctgttg gatgtagtgt
    19861 tgattggagg ggcagtttta tttattggcg gagtcatggt tcatttctgg ctaacgtcaa
    19921 aagacgcatt gttagcttct ttgaatacct tatatccagg aaaacgggta agtactggag
    19981 gaaagtggac gattggcgaa tttttttctt tccttacaaa ttggaaaatt ccttttgaag
    20041 atatcaacta ttcgaataac tctgaagtgg ccctgttcta tcactttttt ccaactgtat
    20101 tcctagcaag tccatttgtg ttatttggca aaaaagatag cgaacaaaag ttactgggac
    20161 gtattttgat gattttttgt ctgttttcta tcttttggat taccgttggg ttgccaaaat
    20221 ggctagcaga aatcactctg ctgtcttatg ttcctccatt aagagccatc cttactttta
    20281 gttttgcggc ttgtttatta tcgatctggt ttatcgctta tatttggaga gaagatgtta
    20341 ttcctatttg gtataaattc attttgttgg cagtaaatgc atggtcttat ttctatgcga
    20401 ttacgcattc gaaaatggat aattattttt cggattttga aaccatcgca gttgctgtgt
    20461 tgggaacagt gattttagct tttgcattat ttggctggag acgcttgttt tacctcgctt
    20521 ttgccgcctt agttatcgta tctggctttt tcgtcaatcc agtcgtagaa ggtacaggag
    20581 cgatttttga aaaaacactg gcaaaagaaa tccaaaaaat cgatcaacaa gatcctggta
    20641 aagtttggtt gtctgaagat gaattatata acttcacccc aacattggga gtgaaatcct
    20701 tcaatacagt acgcttttat ccagatatag ctgcatggaa gaaaatcgat ccgaaagaag
    20761 aatacgagaa gtattataat cggtatgctc atacaagagc ttttatcgct aaggaagata
    20821 caaagttcat tttagatcgt ccagatatgt tttcggtcac gttgaatttt gaagacgcag
    20881 aaaaattagg gatcgactat gtgatcagta aacgaccatt aactgattac aaccaattat
    20941 atgaagcgca gttcacgcag ctatatggac cggataaaga cggttatatg atttttaaag
    21001 tagaatatcc ggattatcca gatgtaccac aaacacaaac tcaggaaaat cagctagtcg
    21061 tacagtaata ggctagctga gaataatcct gaaatgttag tagaaaggag agtggagata
    21121 attcaagcaa tcgtacaaaa atttgtcaac aaaatgagtg tcggtttctt gcttttgtta
    21181 ctgacgctag tcttttttgt ttggacagca gggttaggtt ttccagcaac tttgtttata
    21241 tataacaata gttcattact tatcgctttt agtgtggcta ttttgctcat tttaaatgtt
    21301 tataaagcaa aaggcgtcga ttttctttat ctaggtgttg cgcttgccct tttcattttc
    21361 ttttcttact ttcgggaatt aagaaatgag tctttatttg gagacttact gtttcctttg
    21421 attattttat tttgtttagt aattaaatgg atcgagtgga caaaatggga tcgcggtatt
    21481 tatctgattg tcttttcggt cttcctcgga atcacgttat atcgtgtatt tacagagatc
    21541 caagtaccag aaggacaaag catctgggct ccaggcaatt cgctttctga tatttggatc
    21601 aatacgaata taatcggaag ttctttgatg actcttgctt tattgatcag tggatttgcc
    21661 agttcttttg aacgatggta tgtacgaatc atcggtgcag tagcagttgt tgcagctttt
    21721 ctcgggattt gggtctgcca atctagaggt gcattagtcg cgttgattgt ttttgcgata
    21781 ttggatcttt tacctaaatc gttgatgcgt gtcattcgtg cgccatttat tgcttacacg
    21841 gttacgattc ttttagcttt gccgatttct tatctggcgg ctgtctctga aaaagtcaat
    21901 ttgtttaccg gtcgagaaga tatttggcat aaattctatc agacgctagg agaaaaatct
    21961 gaacaaattt tactaggaat gaaaacattt atcttccaac gaggaaacca atttttagga
    22021 aaccacaata gttataattc tattttgaat atctatggat tgataggatt tgggattgct
    22081 gctttgctgc ttatcctttt tattggtcgc ttaacgttga aagcagattt atccaacggg
    22141 cagatgactt ttatctgggc cttttttgcg gtgatgatgc agtcatttat ggaggataca
    22201 ttgacttcat acccatgggt tccgattatt tatatcctgt tggctatggc cgttcatcga
    22261 tatgatgaac cagaagttgc ggaggaattc tatgttatgg aagaggcata tgacgatatg
    22321 acttatgatg atacatctta tgaggaacaa ccagatagcg ctcattcttc caggatccaa
    22381 cgctatcatt gataggcaca aaatacgaga gaggctgtga caaccgtcgc agcctttttt
    22441 ggtttattgc aattgtctag catcttctgt aaaatagaaa cgttagagaa aatcttgtat
    22501 catagaaaat taagaaagaa ataagaacgc agctcggtac caaaaatatg gtaaaatggc
    22561 acttgactgt aattcactga aaatcgcttg gaggaacaca aatgtttaaa gatttgtatt
    22621 tatttatgaa ggacatttat cagaacagaa aattattgct acagttttcg atcaatgact
    22681 tcaaatcaag atatgcagga tcgtttttag gaatcttatg ggcgtttatc aatccgctat
    22741 ttactgtatt gatttattgg ctggtatttg gtgttggact gaaagcacaa atgatcgatg
    22801 caaaataccc atttatcgta tatttagtta ccgggatgat tccctggttc ttcttctcag
    22861 atgcatttgc atcaactacg gttgtttttc gggaatatac ttatttagta aagaaagtcg
    22921 tattcaatat tcgtatcttg cccacgacga aaatcctatc caatctgtac acccacttat
    22981 tcttcattct gattggcata gctgtcgcgc ttataaatgg tatatatcct tcagcaatgt
    23041 ctttacagct gatttattat ttattttgca tgtgtgcttt cttgacagga ttgacttggc
    23101 tgacggcatc gatccaacca tttttacctg atatcatgca attaattacg atcgtgctgc
    23161 agttgatcat gtggacatta ccgatattat ggagccctaa tcagtttgat tcaggtcttg
    23221 taagcatatt gaaattaaat ccgctgtact atgttgttca aggttatcgt gagtcatttt
    23281 taagtgaagc ttggttctgg gagcatggaa catatacttt gtatttctgg gttttcacgt
    23341 tagtaatgtt cgtaatcggc tctgcagtgt tccgtagatt gaaaccacat ttttcagatg
    23401 tgctgtaagg agaatagcta tgacagaata tgcaattaga ctaaaagata tcacaaaatc
    23461 ttataatatg tacgcaaaac cttcggatcg atttcgtgaa gccctcaatc cttttaagaa
    23521 atcctatcat gatgtatttt atgcgttgaa aaacgtgaat gtagaaataa aaaaaggtga
    23581 gatgatcggt ttcgtgggag aaaacggatc gggaaaatct acaatgttga aaatcattac
    23641 gggagtactg acaccaacat ccggcacgat ggaaatcgaa ggaaaaatct cagctttgct
    23701 agaactagga tcaggattca atcctgagta ttctggctat gaaaatatct atttaaatgg
    23761 aatggttcta ggattttcaa gagaagaagt cgatgaaatg gtcgatgata tcattgaatt
    23821 tgctgacatc ggagaccata tttatcaacc tgtaaaaact tattcaagcg ggatgttcgt
    23881 tcgtctagct tttgctgtag caatcaatgt gaatcccgat attttgattg tggatgaagc
    23941 attggcagta ggcgatcttg aatttcaact aaaatgtatg gaaaaattta cagaaatcaa
    24001 gaactcggga aagacgattt tattcgtttc tcatgatatc agttcgattc gtcgtttttg
    24061 tgatcgttcc tattggttga aaaacggaga agtcgtcgaa tatggcgata cgatggaagt
    24121 aacgacgaat tatgaaaact ttttaaagaa aaaatcagtt aaaacagtgg atcggaataa
    24181 aaaccaagta gaacggttcg ccgctccaga tatcgtcgac gtgacgaaag cggaattgtt
    24241 ggataaagac aaaaatccgg tggatatgct ggaacaagga gaaaaactat acgttaaagt
    24301 aacttatgaa gtaaaagatg atacaatcaa aaatccggtt ttaggaattg cgctacgaac
    24361 agtcgacaat ttttatgtat gcggacttaa tactctacta gatggtatca agattccttg
    24421 gaaaaaagga gagaatatct tttatctgga gtatgaaaag ttaggtttgt tagctggtga
    24481 atattatttt gacgttgcgg tctttgaaga aaatgcaacg gttcctttgg tatataagac
    24541 aaagtacatg aacttgtttg tcagcggttc ttatatcgga gaaggcatcg tggtcttaga
    24601 ccataaatgg gaagaaggga ctcatagcaa tgaaatatga ttttgaaatg gacctagatg
    24661 aacaaagcag tgtgggaaaa attgccgcac agatcaaacc aggaagtaaa gttttagagt
    24721 ttggtcccgg aaacggccga ttaacgaaac atttaatcgg agcaaaacaa tgtgaagtga
    24781 gcattgttga gttagataag gaattgttcg attttgtcag cgaatttgct caagatggat
    24841 tttatggtga tatcgaaagt tttgagtggg caaactatta tgctggacag acatttgatt
    24901 acgtcttgtt tgcggatgtt ttagaacatt tggtagatcc tggaaaaaca ttgaagaaag
    24961 tcagagaatt tttgaatgaa gaaggagaga tcctgattac attccctaac ttggcgcata
    25021 attctgtcat gatcgatttg ttcaataacc aattgccgtg ggcttcttat ggtctcctag
    25081 atgaaaccca taattctttt tatactcatg atggcttcca aaaagttttt gaaaaagcgg
    25141 gattattcat taatattgaa gattatttgt acttagctgt aggtgataca gaattaaaat
    25201 caacttatga agaattgccg gaagctgtac gctatgattt caaaatgcga ccatttggtg
    25261 aagtctacca atactttttc tcattgatga aacatccagt agcacaaagt tccattgcag
    25321 agccacaaaa ttctaattat gtaaaagtgt tagaagtaac ccagcaaacg aaacaagatg
    25381 aaacaatcca acaaatacca ttcaataatt tcacagggga aaatgagaca ctaagtttcc
    25441 ccgtatcaga aacaacagaa agaatggtgt ttcgcttcgc gcaacaacct agctttatcg
    25501 aattcagcgc agaagctgcc ggagaaaaac taacatttat cgattcgaat gctgtagtca
    25561 aaacagtaaa tgattgttat ttgtttgatg ggaaagaatt accagagttc gttctaacgg
    25621 atatttctgg gaaagaagtg actatccatt gtcactatcg ctttatcggg gaattgacac
    25681 caacaatgaa agaattgttg gaaacgattc gtcccatggc tgaagtcaca aagcagcttt
    25741 ctgagaaaaa tgacgaatta gaaagagaaa atcatcatct tcttgaggaa aatacaagac
    25801 tagaccatac gttaaaaaca accacaaatc gctattgcac attgttggaa agtgatgaat
    25861 ggacaataaa acatagacta ggacgacgta agaaagaaac ttcgaaaaaa atccaagaaa
    25921 aagaactttc tatatgtatt gacgagaaaa tctgggatgc agaaacaaaa atattaaaaa
    25981 tcatcggatg gggaatcgct aattcggatc gccagccctt gtcctataag ttatcagctg
    26041 accaatcgcc atttttcgaa gcaattcctg tttcacgaga agaagtcaat caagcagaac
    26101 agctcgcaaa aggaacagaa gcgggatttg agttacgaat cctttgcgag caagaacgtt
    26161 cattcttagt ggaagcagta gctcaaaatg gccaatcatg gatcattgaa atgtaaataa
    26221 tgattaactt ataaagataa ggagtaaacg aatcgtgcaa aaaaaagaaa ttgccgtatt
    26281 tatcgatcaa atcactcgtg aacgggccac tggcgatttg cttattgtgg gctgggcaat
    26341 tgatgaagtg accaaagaaa tccctacgat aaaggtagaa aaagaaaatg tgattgctga
    26401 agcaacacat gtcgtaagat tggatatcaa tcatttatat aatcttgatg ttaagaccca
    26461 aagcggtttt aaggtacgtt tatcagggaa aatgagagga aaagccatct tagacttcca
    26521 aacagcgaga catcaaaatg gcatagctgt gaaattaaac ggtaaatacc cttatgatga
    26581 cggcattgaa tcttcatggg aaagaaaaaa aagattactg aaaaaaggga taaattatgc
    26641 aaggactcat ggcgtaaaaa aagcgatccg tcgggtcaaa ttggaattga agccaggatc
    26701 gatcgactat gcagagtgga tcagtcgtca tgagaaacca gatttgaaag agcaaaggaa
    26761 agaagcacaa ggatttgcgt accgtccatt gatttccata cttatgcctg tctataacgt
    26821 tgaaatcaaa tggctcgaaa aatgcattga ttcggtactg gatcaaacgt atgatcattg
    26881 ggaactatgt atcagtgatg atgcttctac tgatccagct atccgaaagt gtctggaatc
    26941 ctatcaagca aaagatgata gaatcaaagt cgttttccga aaagaaaatg gacacatcag
    27001 tctggcaact aattctgcat tggaaatagc agaaggagaa tttatagcac tcttagataa
    27061 tgatgatgaa ttaccgccat ttgcattata tgaagtggca aaagtgctaa acgtacatcc
    27121 agaactggat gtgatctaca gtgacgaaga taaaatcgat gcagatggca atcgctttga
    27181 tccgcatttc aaagcagact ggtcgcctga tacattgatg ggaaataatt atatctctca
    27241 cttaggcgta tatcgaacaa gcattgttaa agaacttggc ggtttccgta aaggctatga
    27301 aggttctcaa gattatgatt tggtccttcg tgtgacagaa caaatccctg cagatcatat
    27361 ttaccacatt gaccgagtct tgtatcattg gcggacgatc ccaggatcaa ctgcaagtaa
    27421 tggcgaagcc aaaagttata tctatgattc cggagtgaaa gcattgacag atgccctgtc
    27481 tagaagaaac atcaaaggaa gcgtacgtcc tggcagaatt tctggatttt atgagattgc
    27541 ttatgacgtc ttgcaagagg atttggtcag tgtgatcatt ccaacgaaga atggctatga
    27601 agatctaaaa acttgtgtgg attcgatcat cgaaaaaacg tcttattcaa attatgaaat
    27661 cattattgcg gataacggaa gtacagatcc aaaaatgcaa gaattatttg cagaatacaa
    27721 gcatcagctg aaagaccgct ttatcgtaga attgattgac attccattca attattctcg
    27781 aattaacaat ttagcagcag aaaaagcaaa cggcaaatac tttttattct tgaataacga
    27841 tacagaagtg attgagccag attggatgac ggtgatggtt tcttatgcac agtttgatcg
    27901 aatcggctgt gtaggagcaa aactgtttta tccagatgat acaacgcagc atgcaggagt
    27961 attattagga atcggtggtg ttgcagggca tgcattgaat aactatgatc gaacacattg
    28021 tggttatttc ggccgattgg tcattgacgt caattactta gcagtcactg cagcttgtat
    28081 gatggtcaag gcagctgact tcaatgctgt taacgggttt gatgaaacat tagaagtagc
    28141 attcaacgat gttgacttgt gcttgaaagt ttatgaactg ggtcgttaca atgtctatgc
    28201 acatcaagca gaattgtacc atttcgaatc taaatcaaga ggttatgaag atactccaga
    28261 aaaacaaaga cgttttgctg gtgaaatcaa aaaaatgcaa gataaatggc cagcatatat
    28321 tgcgcatgat ccattttaca atgataattt aacaaaagaa ggaatcggcg acttttcgtt
    28381 gagaccagat taaactataa taaaaagctg ttatgttgtg attcgctcgc acataacggc
    28441 tttttatagt tttttgtcaa aaaaaggcag aaggtgccga ctcaaaaaaa taagtcgagg
    28501 ttgggacaga agtgtttaac tccgagaaat aagaagaaat tcacgaaaat tgcttttcaa
    28561 atttttgtga atttcggctt atttaagaag aagttgcttc tgctcccacc gtttattcgt
    28621 ttttaaagaa tgagacaaaa gtgtttctta cttttgtccc acgctcttat tcatgctcag
    28681 agcgccaata gatacaaaat catagccaaa gtttcacgaa ccctagctgt caaagaatcg
    28741 atattttatt ttaatatagg ctattttaaa ataaatggtt taaggtattc ctctacaaaa
    28801 gccactctat caattatcat gggtttaaaa taagaagtga tggagataac cagattgttc
    28861 ttagcgtcaa tataaagaac atttccacta ttaccaattg ctgaatatat ttcgttctca
    28921 gaatctaata cccaccataa atagccatat ctcatatttt gtaagtcttc taaagcactg
    28981 gttctaggtt tgaacatctc gtttatccac atgggtggta cgatttgttt tcctttatac
    29041 atccctttat ttaggcataa tacaccaatt tttgccagat cacttgccga taaacacaaa
    29101 ccatatcctg ccgtcccgat atttagtgga tcacaaaacc agacattggt tttaggagac
    29161 ttatccattg taaactgctt atgttcttca gctgtttcag cataataatt gacatggttt
    29221 ggtatcccta taggatcaaa tagatactta ttggcaaaat caacagttgt tagcttactt
    29281 gtctgataca acaagccagt taatatgtgt aaacagacag tttgatagtt gaattcgccg
    29341 gttatacctt ttcttcctcc caataaatct agtgatgttt ttgtccaatc ttcacttgaa
    29401 cagacttttg tccatggatc gcctttacat ttgtagggtg ctttcattgt tagcaagtgc
    29461 ttgattgtaa tagcgtaaat agttttctcc ccgcgtttca ctttatactc tggaaaataa
    29521 tctaatactt tatcatcgat acttttgatt aatccttgct ctaaagcaat gccaatcagc
    29581 aaagatacaa tacttttcgt tgctgacatg atatgacaag tatcgtcttt tttataacca
    29641 ttccattcat ctgtataaac ttctacccca tccttatatg cgacaatttg acatatattt
    29701 ggttgtcgaa aaccaataaa atcatgcaac tcttgtttat tcataagata ttacctcact
    29761 ttcttctata agcataatgt cattaaaaat cgatttcaat tttttcgatt cttcacgtaa
    29821 tacaggtatt cttatggact agacagcgct ttgggaagat ggattctctg aaaaatataa
    29881 aggttcactt tatgagtgga taaaaaaagc agcaaatact tcgacttctt taatttacca
    29941 acattagtga aaatcgattc atctggagat ttgtaattta gagtccttgt cttgattaga
    30001 ggtgtataaa gctttttatt agacattttt aaattccatt acaatttgct tataaactag
    30061 gtttttaaag gtgactatgg taagataata gagatattat tttgtgatta ggagtgtata
    30121 tatgaataaa aatggagaat ggagtgctgc acgacggata ttcattatat taatggatgt
    30181 tattgtatac aatgcagcaa tatatagtag tttcttactg aagtttcgtg gagagatccc
    30241 attacgaaat ttagaaactt tccaacactc agcaattatt atttcagtga tttttattgc
    30301 gttgaacatt ttgttgggtg catatgtttt ttataatcga atgatcagtg acatcgtgtt
    30361 tgtcacagtc atcggtcagg tattgatgac actagggatc atggtagtga cttttgcggg
    30421 ccgctggttt gcttttccac gtcttgttat cttactttca tttgttctcg gtacgctttt
    30481 cttaagtatc tatcgtattc tcgtatataa actttattta cgcgtaagtc gagataaaaa
    30541 ggtcgtcatt attgggttag aaaaggatgt cgcaccagca atccaaaatt ttggttcaaa
    30601 gaaaaacaac aaacataaag tcgaagcagt cattcttgat aactattatg caaacatcaa
    30661 aaatatgatt aaagacatcg atattgtcta tcttgcttct catatcgaag aaactgaaaa
    30721 aatcaaaatc tatcaattag ttactaaaaa tgaaaaaaaa ttgtttttga atacaacatt
    30781 tgaaaacttg acattgatca atccaaatat tatgaatttc gaagatgaaa gtattattga
    30841 agcatctgga tttagaatcc aacctgaata tgaactattc aaacgtttgt ttgatatcat
    30901 cgtttcactt gttttattag ttattgcttc accatttatg ttattcacag caattttagt
    30961 caaagtgaca tcaccgggac caatcatcta taaacaagtg cggatcacaa aaaatcaaaa
    31021 ggaattttca atctataaat tccgctcgat gaccgccaca gccgaagcaa aatctggacc
    31081 agttttagct aagagcaatg atgcacgagt aacgcctgta ggtaaattta ttcgagctgt
    31141 ccgctttgat gaattaccac aaatcttcaa tgtgctgaaa ggcgatatgt cgatcgtagg
    31201 accacgtcct gaacgtcctt tcttcgtcga tcagttcaat gcagaaaacc cttattacta
    31261 cttacggcat aatgttcgtg ctggtatcac tgggtatgca caagtctatg gaaaatatgt
    31321 ctctgattac aacagtaaat tacgttttga tttactttat atcaaaaaat attcattgat
    31381 gcttgattta aagatcttgc tgcaaaccat caagatccta tttgataaaa tgtcctctca
    31441 aggacttgaa gaagacgaaa atgtgatctc attggcagaa attgcttttc cgaatgaaaa
    31501 aatctttcgc tagttgggag gcgtaaaaat gaacaatccg aaattcagca taatcgtacc
    31561 agtatacaat gcagaagcaa ctattggtcg aacgattgat attctaaaag aattggatta
    31621 tgaagagtac gaagtcgttt tagtaaatga tggatcaaca gatcaaacac aggaaatcat
    31681 tcaagaactg attgaaaatg atgaacgttt tgaattgatt actacgacca atcaagggcc
    31741 cggattagca agaaacgaag gaataagaca gtcaaaagga gaatatctct tattcttcga
    31801 tgcagatgac acgccagtaa aaacgatctt gtcagattat gatcatttgc tagaaaagag
    31861 tccaaaagcg gatcttgttg ttagttcgtt tgtttttcgt acaatggacg agggaagaat
    31921 cgtttccgaa aaagaaaact tagttacaga atgtcactat ctaactcacg aagagtttat
    31981 aaaagacatg tatcagttaa tgaatgaaca gttgatgtat gttgtatgga acaaatgcta
    32041 tcgtagcgat attctgaaaa ataaccaaat tctatttaaa acttataata gttgtgagga
    32101 tcgaatattt aacttacatt attatgaata ctgtcatcaa gtgatcatga acccaaaagt
    32161 agaatatatt tatgaatttg aaggtgggaa ggggatcact aatcaatacc gacctaacaa
    32221 gttcgaaaca tttaaagaat tctattcatt agcaaatact gtaacagacg atattaataa
    32281 agacggtatg gcggcgcttc ttttaaaagg gacaacttca gttatttttt ctatttatag
    32341 tacctctgca agaacttcaa aagagaagaa agacgaagca aagcagatat tgagtgatcc
    32401 agtgatccga gaagccaagc aaattgctaa gacggattct tcagcaaaaa aaataacaaa
    32461 gttgctttac aatatgccag ctccactatt tttatctgct gtaaaagttg gaagcttcgt
    32521 tgaagtgaaa atgccagggt taatggctgc tttgaagcga gtatattagt taatggagga
    32581 aaatataatg aaaagaattt tgcattttca gggacgaatg ggtttaggtg gagcagagtc
    32641 tttcatgatg aatttatatc gtaaaattga tcgtacaaac tatcaatttg attttttgat
    32701 ttatgaagat tatgcagatg ttcaagacta tcattcagag attgaaagat taggaggccg
    32761 tatttttgtt gtacctaatc ctaagaaaaa tatttttaaa tatttaatag aagtgaataa
    32821 gttactaaaa aaagaatctt tttctatagt tcacaatcag gtttattttg gcgggggaat
    32881 taatttatgg ttagccaaaa agaatggaat cagacagaga attgctcata gccatgcaac
    32941 tgaagatgga aaatctcaaa atattgtaat gaatgtattg cgtaaatttt taaccaaatt
    33001 gttgttacaa aatgcaacag actaccttgc cgttagtcaa gaagctggtg aaagtctgtt
    33061 tcaaaaccac ccctttgaga tagttcataa tggtat
//
LOCUS       DAZBTF010000025        31963 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000025 DAZBTF010000000
VERSION     DAZBTF010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.025"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(75..320)
                     /locus_tag="ACHGNS_001260"
     CDS             complement(75..320)
                     /locus_tag="ACHGNS_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFN6937219.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(435..1013)
                     /locus_tag="ACHGNS_001261"
     CDS             complement(435..1013)
                     /locus_tag="ACHGNS_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFN6937220.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(1090..1620)
                     /locus_tag="ACHGNS_001262"
     CDS             complement(1090..1620)
                     /locus_tag="ACHGNS_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFN6937221.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1624..2484)
                     /gene="rlmB"
                     /locus_tag="ACHGNS_001263"
     CDS             complement(1624..2484)
                     /gene="rlmB"
                     /locus_tag="ACHGNS_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFN6937222.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2477..2872)
                     /locus_tag="ACHGNS_001264"
     CDS             complement(2477..2872)
                     /locus_tag="ACHGNS_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFN6937223.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2869..4281)
                     /gene="cysS"
                     /locus_tag="ACHGNS_001265"
     CDS             complement(2869..4281)
                     /gene="cysS"
                     /locus_tag="ACHGNS_001265"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFN6937224.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(4278..4814)
                     /gene="epsC"
                     /locus_tag="ACHGNS_001266"
     CDS             complement(4278..4814)
                     /gene="epsC"
                     /locus_tag="ACHGNS_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFN6937225.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(4900..5139)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(5136..6596)
                     /gene="gltX"
                     /locus_tag="ACHGNS_001267"
     CDS             complement(5136..6596)
                     /gene="gltX"
                     /locus_tag="ACHGNS_001267"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFN6937226.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6766..7923)
                     /locus_tag="ACHGNS_001268"
     CDS             complement(6766..7923)
                     /locus_tag="ACHGNS_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFN6937227.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7957..9327)
                     /gene="radA"
                     /locus_tag="ACHGNS_001269"
     CDS             complement(7957..9327)
                     /gene="radA"
                     /locus_tag="ACHGNS_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFN6937228.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(9390..9917)
                     /locus_tag="ACHGNS_001270"
     CDS             complement(9390..9917)
                     /locus_tag="ACHGNS_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFN6937229.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            10147..10953
                     /gene="proB"
                     /locus_tag="ACHGNS_001271"
     CDS             10147..10953
                     /gene="proB"
                     /locus_tag="ACHGNS_001271"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFN6937230.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            10950..12197
                     /locus_tag="ACHGNS_001272"
     CDS             10950..12197
                     /locus_tag="ACHGNS_001272"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFN6937231.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            12273..12617
                     /locus_tag="ACHGNS_001273"
     CDS             12273..12617
                     /locus_tag="ACHGNS_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937232.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(12734..13060)
                     /locus_tag="ACHGNS_001274"
     CDS             complement(12734..13060)
                     /locus_tag="ACHGNS_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937233.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(13675..14877)
                     /locus_tag="ACHGNS_001275"
     CDS             complement(13675..14877)
                     /locus_tag="ACHGNS_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN6937234.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            14994..15509
                     /locus_tag="ACHGNS_001276"
     CDS             14994..15509
                     /locus_tag="ACHGNS_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFN6937235.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(15708..16154)
                     /locus_tag="ACHGNS_001277"
     CDS             complement(15708..16154)
                     /locus_tag="ACHGNS_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFN6937236.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(16201..17679)
                     /gene="msr(C)"
                     /locus_tag="ACHGNS_001278"
     CDS             complement(16201..17679)
                     /gene="msr(C)"
                     /locus_tag="ACHGNS_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFN6937237.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(18033..18662)
                     /locus_tag="ACHGNS_001279"
     CDS             complement(18033..18662)
                     /locus_tag="ACHGNS_001279"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFN6937238.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(18646..19110)
                     /locus_tag="ACHGNS_001280"
     CDS             complement(18646..19110)
                     /locus_tag="ACHGNS_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFN6937239.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(19597..20865)
                     /locus_tag="ACHGNS_001281"
     CDS             complement(19597..20865)
                     /locus_tag="ACHGNS_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFN6937240.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(21096..21833)
                     /locus_tag="ACHGNS_001282"
     CDS             complement(21096..21833)
                     /locus_tag="ACHGNS_001282"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN6937241.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(21900..22469)
                     /locus_tag="ACHGNS_001283"
     CDS             complement(21900..22469)
                     /locus_tag="ACHGNS_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFN6937242.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            22597..23469
                     /locus_tag="ACHGNS_001284"
     CDS             22597..23469
                     /locus_tag="ACHGNS_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN6937243.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            23811..24683
                     /locus_tag="ACHGNS_001285"
     CDS             23811..24683
                     /locus_tag="ACHGNS_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFN6937244.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            24680..26638
                     /gene="nagE"
                     /locus_tag="ACHGNS_001286"
     CDS             24680..26638
                     /gene="nagE"
                     /locus_tag="ACHGNS_001286"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFN6937245.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(26970..27821)
                     /locus_tag="ACHGNS_001287"
     CDS             complement(26970..27821)
                     /locus_tag="ACHGNS_001287"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN6937246.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            27944..28411
                     /locus_tag="ACHGNS_001288"
     CDS             27944..28411
                     /locus_tag="ACHGNS_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFN6937247.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(28674..29156)
                     /locus_tag="ACHGNS_001289"
     CDS             complement(28674..29156)
                     /locus_tag="ACHGNS_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFN6937248.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(29256..30119)
                     /locus_tag="ACHGNS_001290"
     CDS             complement(29256..30119)
                     /locus_tag="ACHGNS_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFN6937249.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            30717..31592
                     /locus_tag="ACHGNS_001291"
     CDS             30717..31592
                     /locus_tag="ACHGNS_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFN6937250.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(31737..31889)
                     /locus_tag="ACHGNS_001292"
                     /pseudo
     CDS             complement(31737..31889)
                     /locus_tag="ACHGNS_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
BASE COUNT         9248 a         6722 c         5749 g        10244 t
ORIGIN      
        1 ttttttattc ttagcatacc gattcgcaca caaaaaagca aacgaacctt acgaatctca
       61 ggctcgtttg ctttttaaac tgcttcagtt aaaaattcga tctcaactgt ccgagtaagt
      121 acatctgaat agctgtaaga tactcgttca aaagaatttt cttcaggatc caaatcaaca
      181 acgaatactg aaggataggt ttctgttaaa acgccgcggc gttctgtttg acgctttctt
      241 cctgtctgag caaccaacat gattgggctg ccgatacgac cttctaaatc tttcttgatt
      301 gaagctaaag ttgttggcat acagttcacc tctactatca actataccac atatttttaa
      361 aattttcaag tttaccataa aaaaaaaaga aatgcaataa aaataaataa gaagctcatt
      421 aagatttttc taaattattc ctcattataa aatttcaagt gtttttttat tttgagtttt
      481 actctcccgt aagcgctcat tacttgcttg tgatccattg ctaaacattc agccatgtct
      541 acaaaatcaa cgccttgcat ataataatga aaaattttag cttctatttt tgaaaactgg
      601 atttcgcaat tttcgattgc ttcttttaat aaaagatgct gaattgctaa aggttcttgc
      661 aatgtgatct ctgctaaaaa gtctggtcct tctgtctcta ttttttggtc gattgagact
      721 tcatcttttt gatcacgacg cttcagcgcg ttctgcttcc tcaacagaga atagatttga
      781 ttgcttataa cgcttttata ataacttcca aagctaacac ctttgctttt ttcgtaagtt
      841 ctcaatgcct gatataaagc gatccttgct tcttgttccc aatcctcaga aacataattt
      901 cttaaataat aggtgttttt caaacgtagt aaaagtggaa aatatttttg atacagggca
      961 gcaaatgctt cttcgtcacc attacgtgca gcgcgcaata actcctcaga cataaaataa
     1021 ttcctccata aattcgttta tggaggaatc ttatcatcaa tgattcatcg aactgattag
     1081 aacgatatgt tatttatttt ttctcattaa atcgtccaat ttttgggaca atttttttaa
     1141 ctgctcttca ttccatggag aattacgtcg gaaatcaata aaatgcatat ctttattttc
     1201 ttgggctatt gttttttccg ttttttttat cgtcttatat aattcataag cagaagttcg
     1261 taatgctcct tgggaaaaga tcatccactg ttctgctaaa tcgcttgtcg ccactgtcac
     1321 ttgtgtcaaa cggtcattca gctgaccagc gattctctcg atataactgt cagccgtttc
     1381 gtcttctttc gtaaaaacaa catccaactg gtatttctta taattttgtt ggatgcccgg
     1441 aactagttgg gcatcgaaga caacgatgac ttccattcct tcatatttcg cataatttga
     1501 taaacgatgt aaaagagctt ctcttgcatc cgccattttg tcctgctttt taagctggac
     1561 aagttccggc cacgaaccaa tcatgttgta gccgtctaca atcaacagct gttttttcat
     1621 ggtctactcc ccctgacgtt gggaaaaagc ctgatacata agcaaaccag cagcaacact
     1681 ggcatttaag ctctggacgt gcccggtcat cgggatcgtc agcaattcat ccatttcttt
     1741 tttcaatcct ggactcattc ctttgccttc attaccaatg actaaagcaa ttgatccttt
     1801 ggcattccat ttttggaagt ttgtgccggt catatctgtg ccaaataccc aaaatcctgt
     1861 ttctttgagc tgttgcacag cttgtaccaa attcgtcacg cgtgcaacag ggacatgttc
     1921 gactgcccca gtggaagtct ttgttacgat aggtgtgatt ccaactgctc ggtgttttgg
     1981 tatgatcaca ccatcgacgc cacttgcgtc tgctgtccgt aaaatcgacc caaaattgtg
     2041 tggatcttcg atgctatcca aaatcaaaaa gaaggggtct tcttttttct cttttgttcg
     2101 ttcaatcagt tggtcaaggg ataaatattc ataaggtgta atcgcaagaa cgactccttg
     2161 atgcacgccg tgatcgctca aagtttctaa tttttgttta ggaacccatt tgactggcac
     2221 agcttgttca cgtgcgattt ctttcagttc ttctactttt tctcctcgtg aatcttcttg
     2281 aagaaagagt ttattccctc gcccttgttg tagcgcttct actgcggcat ggtgcccaaa
     2341 gacgaaatta tcttctaatg cttcttcatt ttgatcagaa actgactgat ttcgtccagt
     2401 ttgcttttta tttgaatatc tattttgttt tttgtcttca aatccacgtt tcgattgatg
     2461 tttgtttgtt ctttctttac ccattttttt caccaacttt ctgaatacac cagcggatca
     2521 attcttccat ccgctcggtt tgttttgtca aatgcagata gcccatgagc gcttcgaacc
     2581 ctgttgcgac ccgatacgtc gtaacatcag cattttttgc gctcgtatgg cttttagcat
     2641 ttcttcctct gcgataataa agttcttctt cttctgtcaa aagatgttct tccagcattt
     2701 gctgaatcaa aaaggcttgt gcattcgcag aaacataatt cgttgccgca cgatgcagct
     2761 tattgggttt tgtttctcct tgatcaatca ggtggtcacg aacatagatt tcgtaaatcg
     2821 catctccaac ataagccaag gctaaaccgt tcaattgtgt gtaatctctc atgcttctct
     2881 tctccatcgg gttccttgcg gtgtatcttc taataagatc cctttttctt tcaacaaatc
     2941 acgaatttcg tcgcttcgga caaaatcacg attgcttctt gcttgatttc gttcttcgat
     3001 caattgttca atctcttcat ctaataactc ttctgctgta aataaaatac cgaagatcga
     3061 aagccattgc gagaataaag cttctccttg tgccaagaca gcggcagaaa ctttcggctg
     3121 gtctagataa gtgttcagcc acttagcaag ctcatagacc actgtgatcc catttgctgc
     3181 attaaaatca tcgtccattt cttcgatgaa tcgctgttct aaattttcta acaaagcgat
     3241 tttatccgca tcatctgcca aattctctga ggcagtttct tggcggaatt tcaaattgtc
     3301 aaacgtattc ttcagtcgtt gtaaattggt ggctgcttct ttcatcgtcg tttcactgta
     3361 gcggatcgga cgacggtatt gtgtggtcgc catgaagaaa cgcaagactt gtggatcaac
     3421 gtttttgatc atttcatgta cagtgatgaa attccctaga gatttactca ttttttcatc
     3481 gtcttcgcca atcgttacat agccattgtg catccagtaa ttagcgaatt ttttccctgt
     3541 tttcgcttca ctttgtgcga tttcattttc atgatgagga aattctaaat cttgcccgcc
     3601 accatggata tcgatcgttt ctcctaaatg ttttgttgcc ataacggaac attcaatatg
     3661 ccaaccggga cgtcctttgc cccatggtga atcccaagaa atctcgccag gttttgcttg
     3721 tttccataaa gcgaaatcta atgggtcttc tttttgctgc tgttcttccc ctgtacgctg
     3781 acttgctccg acttccagtt catcgatgga ttggtcactt aatttcccat aattttcaaa
     3841 tttacgggta cggtaataca catcacctga agattcataa gcatagcctt tttcaaccaa
     3901 cacttgaata aatgctaaaa tatctggcat atgatccatg actcttggat gcagtgtagc
     3961 aggttcgaca ttcaacgctt tcgtatcttc ttcaaatgct cggatgaaac gatcagcaac
     4021 ttctggtgct gtaataccta attcgtttgc tgtacggatg attttatcgt ctacatccgt
     4081 gaaattcgat acgaaatcga ctttatagcc tcgatattca aaatagcgtc gaattgtatc
     4141 gaaagcaatg gtgctacgag catttccgat atggatataa ttatacacag tcggtccgca
     4201 gacatacata ccgacatgtc ccttttgtat agggatgaat tcttcttttt ttctggtcaa
     4261 tgtattgtag atttttatca ttctgatctc ctcacttttt ctcctttgat tcgaacgact
     4321 tttgctggta ctcctaccgc tgtagcgtcg tctggaacat ctttcaacac aacggcacct
     4381 gcaccgattt tggcgttttt tccgatcgtt accggtccta atatctgcgc attcgcagag
     4441 agcatggctc cttgtttaac agttgggtgt cttttacccg tttcttttcc tgtgccgcct
     4501 aatgttacgc catggaataa aatcacatcg tcctcgattt ctgcggtttc gccaatgacg
     4561 attcccattc catggtcgat gaacacgcca gttccaatcg ttgctcctgg atggatctca
     4621 acaccagtta ggaaacgcca gaattgagca ttgatcttcg ccaataaaaa gagccggtgc
     4681 tgatataaaa aatgagagaa acgatgccaa aacaaggcat gaaaaccagg gtatgtcaaa
     4741 acgatctcag ctgtcgaacg tgcagccgga tcgtttctct ttgctgcatc aatcgctcgg
     4801 gtaaaccaac tcatgacttt ccactccttt cgtaagaatt ttatgtagga aatcatcttc
     4861 ttataaagaa aagaagcgcc ttttaaataa gtttccttat caaaagacgc tttccgcgtg
     4921 gttccacttt tcttcataat caatgattat ctctgacagc taacgcctgt tatacgttct
     4981 tggctacttt gtttcctata aaacagtttt tcctgttttc tgtgtttcgc caagaccact
     5041 cccagaggca tttcggcaac cattcattga taccttgcac cacccggtat ctctcttgta
     5101 atgatcagct gcttactttt ttctgttcat cgtgtttagt tcagtacttg atttaaatgt
     5161 gctaatgatt tttcacgacc aagcaattcg atcgtttcag gcaattctgg accatgcatt
     5221 tgtcctgata cagctacacg gattggcatg aaaagatttt ttccttttac gccagtttct
     5281 ttttgaacag ctttgatggc agctttgaca gttggagcat ctactacttc catttgttct
     5341 agctgtgctt tgaatgcgtg gagaacagta ggaactgttt caccagcaag tacttctttt
     5401 gctgcttcat tcaatacagg atgttcattg aaaaatagcg tagacagatc aacgatttct
     5461 gcagcatagc tcatttgagg ttgatacagg ctaacgatct tcttcaccca ttcgattgtc
     5521 ttttcatctg ggttttctgc tacacgtcca tcagcgatta gataaggcag acacatgtca
     5581 gtaaatgcat ctagatccat tgctttgatg taatggttgt ttacccattg cagttttttc
     5641 gcatcgaaag ctgcgggtga tttacttagg cgttttggat cgaacatttt gatcaattct
     5701 tggtgttcga agatttcgtc ttcgcccaca ggtgaccagc ccaataatgc aataaagttg
     5761 aacattgcat ctggtagata gcctaattcg cgatattgtt cgataaattg caagattgat
     5821 tcgtcacgtt tgcttagttt tttacctgtt tcactgttga tgatcaatgt catatgtccg
     5881 aaggttggtg gtgtccagcc aaatgcttca tagatcatca gttgttttgg tgtattggca
     5941 atatggtcat ctccacgtaa aacgtgagtg attttcatca aatgatcatc gaccgcaaca
     6001 gcaaaattat aagttggcat accatcgcgt ttttggatga caaagtcgcc accgatatta
     6061 tcggattcga acgaaatagc gcctttcacc atatctgtaa atgcgtattc tgtattgcga
     6121 ggcacacgga aacgtacaac agattctaga ccttgtgctt ctttttctgc ttgttcttca
     6181 ggtgtcaagt tcgcacatgt tcctgcgtaa tgaggcattt ctcctcttgc gcgttgtgct
     6241 tcccgttctg cttctaattc ttcttctgtg caatagcatt tgtatgctcg attgctagca
     6301 agcagctgat caatcagcgg ttgatagatt tctttacgtt cagattgacg gtaagggccg
     6361 tattctccag gattttcagg agattcatcc caattcattc ccaaccaagc aaggttttcc
     6421 aactggcttt tttcgccatc ttcgatatta cgtttttggt cagtatcctc aatacgaata
     6481 ataaagtctc catcattgtg gcgggcaaac aaataattga ataatgcagt tcttgcattt
     6541 ccaatatgca ggtgcccagt tggacttggt gcatagcgta cgcgtacttt cgtcattttc
     6601 ttcctcttct ttcaaactag actttgacta acagcaaaaa ttccgtcagt tacggcttct
     6661 caaatatcat ttatttaaag ccactcagca caaattttac cttttctaag gtaaatagta
     6721 aagccaactg gttgaatttc cttaccagtt ggctgaattt cttttttatt ttgattgttt
     6781 agtctttttc tctgtctctt ttttattgtc gattcctcgt cctgcatgtg caggtttagc
     6841 aaagatcatg cgtccagcag ctgtttgaag ggcacttgtt acaacgactt cgatacgttc
     6901 attcatgtaa tgctgtccat cttctacgac aaccatcgtg ccgtcatcaa ggtaggctac
     6961 tccttgttgg cgttctgtcc cggcttttac tactagaaca ttcatcgttt caccagggat
     7021 gacgactggt tttacggcat ttgccaacgc attgatatta agaacaggta cattttggaa
     7081 ttcagagact ttattcaagt tatagtcatt ggttacaatg acaccatcta aaagcttagc
     7141 caatttgatc aatttgctgt ctacttcact gatatcttca aagtctccat cgtacatttc
     7201 gacagatata ccctcttctt tttgcaaggc atttaggata tctaatccgc gacgaccacg
     7261 cacacgtttc aaactatccc ctgagtccgc gatgtattgt agttcgtaaa gaacaaaatt
     7321 agggatcatc aaggttcctt ctaagaatcc agtctttgta atatcataaa tccgaccgtc
     7381 aatgatcacg cttgtatcca agattttata tttatggaaa ttttctcctg attttcgttc
     7441 taacagttga ctctcaactt gactagctgt ttcttgctcg atttttttgc ttcgtgaacc
     7501 aaaaactttc ttccattctt cgattcgtgt cgttcccatt ctcaatccca gatagcctaa
     7561 gatgatcatc agtaaaatcg gcaatacact attgacgaat ggaatttcca tattgtaaag
     7621 aggggtcgag atcacaacac ctaaagtcaa tccgatgatc gcaccgatcg caccgaagag
     7681 aagataagtc aagctgacct cacttagttt ttgttctact tgtttgataa tagctgagat
     7741 atgtttggcc aacagcaaag ataataaaaa gaaaataagt gcgcctaaca ggctgttggt
     7801 aaatgtatta ttcagccatc cgtttgactg ttgaccgatt gcctgccaag ctgagggtaa
     7861 aaatgaaata cccaagcttg cgccgacaat gatcatcagc aaagtaatga cacgtttttg
     7921 catcgttttt cctccgtttc gacaatcatt tattagttag cggaacacac gcttcaacgt
     7981 ttcagccaaa gttgctactc caactatctc gattcctttt ggcgcttccc agttacctaa
     8041 gttgttttta ggtaggtata cttttgtgaa gccgagtttt tggacttctt tgactcgttg
     8101 ttcaatatga ctgacacgtc tgatttctcc agtcagtcca atttcaccga tgaaacattc
     8161 cgttggctgt gttccttttt ctttatagct tgaagcaata ctgacagcga tagctaaatc
     8221 aatcgctggt tcatttaatt ttaccccacc tgcagctttt aagtaagcat cttgattctg
     8281 aagaagcaac ccagctcttt tttccagcac tgccatgatc agagataccc gattgaaatc
     8341 aagacctgtt gttgttcgtt tggcatttcc gaacatagta ggtgtgacta atgcttggat
     8401 ctctaccaaa attggacgtg tcccttccat tgccacaacg atagcagagc cagttgcacc
     8461 gtctaggcgt tcttctaaga aaatctgtga aggattagcg acttcttcca atccatgctc
     8521 atgcatttca aaaatcccaa tctcattcgt tgagccaaat cggtttttta ctgctcgcaa
     8581 aatccgaaaa ctatgatgtt gttcaccttc aaaatataac accgtatcca ccatatgttc
     8641 taacattctt gggccggcga tcgagccttc tttggtaaca tgaccgacga taaaaatggc
     8701 tatcccattt gttttagcgg ttttgagtag ttctgcagtg gtttctctta cttggctgac
     8761 acttcctgcc acacttgtga tgtctggctg cgtcatcgtt tggatggaat caatgataac
     8821 ataatcaggg gagatatgtt cgatggcacg actgatttcg ttcatatctg tttcagcgta
     8881 tagataaaat tctgtgttga tacttccgag gcgttccgcg cgtaatttta tctgctctgc
     8941 gctttcttcg ccggatacat ataagacttt tccgcctatg gctgctaatt gctgagaaac
     9001 ttgtaacagc aaagtggatt tcccgatacc tggatcgcca ccaatcaaga ccatagaccc
     9061 tggcaccaca cctccgccta acacacggtt cagttcttct aattttgttt tgatccgagg
     9121 ttctttttta gggacgacgt ctgcgatttt tgttggtttt gctttttctc ctgtcaaact
     9181 tgtccgagtt ctgcggtccg ttgtatcttg ttcgatttct tcgaccatcg tattccattt
     9241 tccgcaattt gggcagcgtc ccaagaactt aggcgacaca tacccgcaat tttggcaaac
     9301 aaattgtact tttgattttt ttgccattgt accgcctcct tttatcctaa tcatgagaca
     9361 ttaaatcaag cttaagtatc tttaacgttt tatttactgg aagaaccaaa tccgccttga
     9421 cgttgagcag tcgtttgatc ttgatctgct tttaaaaacg gcaaaaagat cccttggccg
     9481 atacgctcgc cttttttgat cactacatcc ttgaaaccaa agttggtgaa ttggaacatg
     9541 atatggcctt catttgcctc attattgtaa taatcactgt caatcacacc tactccgttt
     9601 gtcaacacta aaaaccgctt caacggattg cttgaacggt tagcaagctg caaaaattca
     9661 tcttctccca tgtaagcttt gattccagta ggcacgaggg ttggtttcaa ttgtttttca
     9721 ttttctgata catctttttt caatccatct gcgactgttt ccaacccctt tttccagcct
     9781 gctttccaga tacttggaat cacgacctct tctgctgctt caaaatcata gccagcagca
     9841 tgatgtgtag cgcgctgcgg tagattgata ttttgatctt tatatttaga aataacttca
     9901 aatcctcttt ttctcaccgg ttttcctccc taaataaact actcctattc taacatatcc
     9961 gaggctgtaa aagactttct ttacaaattc ttcaagaaat tctgctatct taaagtaaac
    10021 cattaattcg ttctttttcc aaacgactgc aagttgtaca aaaaaactat tttttacatg
    10081 actttactcg tcaaaacaag tagaatagaa cgaaattaat gagtggatat ttgattgagg
    10141 tgtcacatgc gtaaagaaat taaaaatgca aaacgaatta ttgtcaaagt aggaacaagt
    10201 acattgattt atcccaatgg aaatatcaat cttgcggcaa tcgatcagtt agcttttgtc
    10261 ctttctgatc ttagaaatcg gggaaaggaa gtgatcttag tctcatcagg agctgtaggc
    10321 gttggtatgc ataaattaca tttgtcagaa cgtccgactt tgattcctga acagcaagct
    10381 gcggcggctg ttggtcaagc cgaactgatg aatatctata atcaaagatt cagtgtatat
    10441 aatcaacaga cggcacagat cctcgtgaca agagacgtgg tagattatcc tgaaagtcgt
    10501 aaaaatgtga ggaatacatt ggaacagctt ttgattatgg acacgatccc tatcgtgaac
    10561 gaaaacgata ctgtagcagt ggatgaatta gatcatctga caaaatttgg cgataatgat
    10621 cagctgtcag ccatcatggc aaaacttgtc gcggcagacc ttttgatcgt cctttctgat
    10681 atcgacgggt tttattcaga taatccgact acaaatcccg aggctcagat gtatcgctat
    10741 atcgataaaa tcgatgaaaa gattctggca caagcaggtg gtgctggtag cacatttgga
    10801 acaggcggta tgcagagtaa attaaaagcc gcttctagga tatttgaagc aaacagcgca
    10861 atgattttag caaatggaaa aaatccggca attatttttg atattcttgc aggaaaagaa
    10921 gtaggtactt tatttaagga ggacttgaaa tgactgactt gatgcaatta ggacaacagg
    10981 caaaagatag cgcgcaacaa ctagcactta tgcctacaaa ccgcaaaaat gagctacttg
    11041 ttcaaatggc acaaaccatc aaaaagaatc aacaagcgat cattgaagca aatcataaag
    11101 acttagaaaa agcagtcgaa aacaatatca gtgaaacgat gcaagaccgc ttgcagctga
    11161 cacctgagcg aatcaatgcc atggctacag agatcgaaaa aattgcttcg ttagacgatc
    11221 ctattggaaa agtagatgag atgtggacga acaccgacgg attgagaatc ggcaaaaagc
    11281 gcgtgccact aggcgtgatc ggcatcattt acgaatctcg tccaaatgtc acgacagatg
    11341 cagctagtct agcattcaaa tcagggaatg ccgttatcct tcgcggtggc aaagaagcat
    11401 tcttctcgaa tcaattatta gtccaattat tgcagcaagt actggtcagt gctggcgaat
    11461 ctccttatgc catccagttt gtcgatgata cttctcatga aactgcagga cagttgatgc
    11521 gattgacaga atacttagat gtgctgattc ctcgaggcgg agcaaaatta atccaacgag
    11581 tcaaagaaca ggcaacggta cctatcattg aaacaggtac aggaaataat cacgtatata
    11641 tcgataaaga agcacagtta tctatggcag tgaacatcat cgtcaatgca aaagcttccc
    11701 gtccatctgt ctgtaacgcg gcagaaacat tgctgatcca ttctgagatc gcaccttttt
    11761 tcttgccagc tattgaaaaa gaactggtag aacacggggt ctctttacgt gcggatgctc
    11821 gtgcgttaga atatctagag acggctgctt tagcagaaga cacagattgg gatactgaat
    11881 atctagatta tatccttgct gtcaaagtgg tcgattcttt ggatgaagca attgcacata
    11941 tcaatcggca taatactaaa cattcagaaa cgatcgtgac agatagttat gcggctagtc
    12001 aacgtttctt aaatgaagtg gacgcggcgg ctgtctatgt caatgcctcc actcgtttta
    12061 cagacggatc ggtatttggt tttggtgcag agattggtat ctctactcaa aagttgcatg
    12121 cccgtggtcc aatggggtta aatgaattaa cttccacaaa atatatcatt tatggcgatg
    12181 gacaaatccg cgaataatgt ttcgtgtgaa acattattcc cgccttttgc cttttttctg
    12241 taaaggttct atccaactag aaaggtgtca atatgtttga aattttaatg atttttttta
    12301 tttaccttat ttctctgaat atcgctgctt ttctgggagt cggtatttta tcccttttct
    12361 ttcaattcaa aaaacgaagc atagggagtc aacgggaaaa atggtcgcag tattttgaca
    12421 aaatcgggca aaaagggctg gttacacgct tgcacattag ttacatggtt gcattatgct
    12481 tattagcgac aatcaattac tattcttttt tcgatcattc aatcgcttat acgattacgc
    12541 tactgattgc tgggattttt cacttatctt ataaatatca gctgaataaa aaccacttga
    12601 atcggacttt ccgctaaaaa atatccgtac gataagtaga gtgccttgaa gatcggcaaa
    12661 aaactgccgg actttttaga ggctgtgaca aagattttgt cacagcctct ttttccatat
    12721 aaacggcgtt cgcctaccag ctgcctggta aatctgtttc ctcttcgtta agaagcgaaa
    12781 atccgtcaaa tgtttcgcca tcataataac gaagcagctc atccatggac aagttcatcg
    12841 ttttttcttt ttcttcttcc gctgattcag ccagtagctg ttcccgatac acctgataca
    12901 taccagctat tttttgctgc atcgcttttc tagtttcacc ttccgcattg attattggca
    12961 attctttgat atagccacga taaatcaacc gatcatctga aaattcaatg ggtaattcaa
    13021 cgagtgtcaa cccgacttcc tgaaaatatt cttccagcat ctattatccc cccattcttt
    13081 ttcttaaatg aattttagct tacctactga aaattttcca gtttcattat tttgagaaaa
    13141 aaagggaatg tgacaaaaat cgtgtcacat cccctttttc cgaataaatg gtgttcttct
    13201 gccaactcct caatcataag tcacaatatt caacaaacat gagaaactgt tttttgaaat
    13261 tgttccttgt gattcagagt taaacgacag tctcacaacc tcttggtaaa cggcgttcaa
    13321 tcagctgacc ctcggtatta gctcaaaaaa cggtaaaata tgagaagcta tttttccgtt
    13381 ttgtttctct aatacttagg ataaaacgct gattttacaa cctctgttaa acggtgttca
    13441 atcagctgtc cttcggtatt agctcaaaaa acggcaaaat atgaggaact atttttccgt
    13501 ttgtttctct aatactcagg acaaaacgct gattttacaa cctcctgata aacggtgttc
    13561 aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aatatgagaa gctatttttg
    13621 tttggctgtt cttatgactt gatgcaggac agctttttca caacctctga tttcttattc
    13681 ttcttcattt gtagcaaaat aattacggtg ataaatgata caacatacac tgataagaac
    13741 aatggcacct gcaaaataaa acgcatagct ttcaccaata tgttcaccaa tcacacctgc
    13801 aataaatggt gctaaagctc caccgaaaaa gcggacaaaa ctatagctgg acgaagcaac
    13861 gtttcgttgc agacctggta tttccatagc aatcgtcgtc aacagtgtat tgataattcc
    13921 ttggaaaaat cctgctaaca caatgctgat cggtacaagg actggcatag aagttccagc
    13981 tcctaataac agcaaacaaa taatgaataa gacaaatgct gctaaaattg tccgataagt
    14041 cgtaaacttc cgctcaagag ccggtgcaac gaaaatcgaa gcaatagcca gaagcacacc
    14101 ccagccaaaa aatacaaaac cgacttctaa ttcagaatag ccaatcaata aaaatggcgc
    14161 atacgcaagt aaagtaaaga atccgaaatt atataacaat gcgattagtc caactgaacg
    14221 aagtttatga ttgcttaacg ctttgacacc ttcccaaagg cctacttttc tctttggttt
    14281 ctcagtttca ggtaaaagta ccgctagggc tataaaaccg caaaacatca aaaatgctac
    14341 cccaaaaaat ggataacgcc atgaaccact gcccaagacc ccaccgacta gagggccaac
    14401 agccattccg cagccaagtg ctgcttcata catcatgatt gctttttctg tgttaccaac
    14461 taaaatactg acgatcgttg ctaaagcagt agagacaaat aaagcattcc cgacacccca
    14521 gccggcacgt aacccaatca gcattccgat attcccagat aatccgccaa gaccagcgaa
    14581 tacaacgatg ataaacaatc ctgtcatcaa tgtttttttc aaaccgatcc gactagataa
    14641 gaaacctgta aatagcatca ccacaccagt gactaacatg taactagtaa ataacagggt
    14701 cgtttcagct ggcgtagcgt ttaactgtgc agcaatcgtt tttaagattg ggtctactag
    14761 acccactccc ataaaagcga tcatacaggt agctgctaca actagcgctg aacttttccc
    14821 ctttgtttct tcatttgttt gttcttgtat atctatttga ttctccaata tttccatccc
    14881 tttctctcta ttagcatcat acatgattta ttttacatgt caatatgcac atgtaaaacg
    14941 tggaaacttt actgtgatat aataaagaaa ataagacgag aggtaggatt tttatgaata
    15001 ctttagccta tatattatta tgcgtgcttt tatccaaacc acgttctggg tatgaattga
    15061 agcagctgat cacaattttt tgggaagccc atcacagtca gatctataca acgcttgcca
    15121 agttagacca gcagggttat gtgaccgttt tggacaaaga cgagcactct caaaaaaaga
    15181 tctaccactt gactgatgaa ggacgtgctc ttgtagagga atggatcaaa gaggaaactc
    15241 ccgctcctac gcaaaaagat gaatttctag caaaaatcta tgctttttcg acgctcgata
    15301 aaacaacagc tactggactg ctattcacta gagaacaaca gctgaacaaa atcatgcaaa
    15361 aaaatcaaaa aaaattgaat gacctctcgc tagacaagaa acaagaattt ggacgttatg
    15421 ttgtgatcca gcgcagaatt atgctttgtc agcaggaact ccaatggtgt cagcaagtaa
    15481 gagaacaaat gcagcagttt tttgagtaag ggataaacag aacctttcta gaaatatcta
    15541 ttgaagagca aattatcttc aataaaagag caatacagtt ggaaaagaaa aagcgatccg
    15601 aacgacaggc caaaacctat cgttcggatc actatttttc tagaactgca ttcaagagac
    15661 tatgacaaaa agcatgccaa ggcctttttt caaataacag tgttccatca ttcattactt
    15721 tgaattgctt ttagcacact tcttgaaagc gggccaacga tgatcaactg caacggtaaa
    15781 gcggcaatca cattcatttt ccaagcattc atataagcag agaataattg atcaggtatt
    15841 ccttgttcca tgatcacacc gaataaagac atacatgtca ccattcctaa aaccatcaag
    15901 catgaaatag ttaagatcat ttggaccttg ctttctggat ttactggtag tttaaaggca
    15961 atttttttcg ccagcacacc gatgatgaag acatccaaaa tgaaagccac cacgaatcca
    16021 ggcactagac caatcaacaa acttgataaa tgaagatctt gatgcagcca taaattatac
    16081 gcgctcatcc ccaagaccat caaaaaacac atcattgttg taaaaattat tccttctttt
    16141 ttatttgtag gcaatctgta ccactccttt aaatttgtca cgaaaatcat cgtagcacat
    16201 ttaaaaattt ctcgtaagta ctttttttat tttctgccct tttatttcat aaacttcatc
    16261 agccgtacgc tcgacaaagt aagaatcatg agaagtaaac aaaatcgttc ccggatatat
    16321 ttgcattagc ttctctaaag cttcgatcgt tgccatatca ataaaattag tcggttcatc
    16381 taacagcaac acattacttg gcttagtaaa aagcaaagca atcgtcagac gggtcgcttc
    16441 tcccccactt aatgtacata acggtttcgt caaagtttcc tgagcaaacc ctaagtgatt
    16501 cagcaaacta cggacaagcg attctgaaaa ctctgtttgt ctcatgacaa aacgaatgat
    16561 ggattcttca gatgtcattt gataaccctt ttgctgatat acttgaaagc ttactttcgg
    16621 agagagaagg attccttctc cttgtttgcg gatatgttct aataaggttg tcttacctga
    16681 accattttca ccgacaatcg cgattttttt acctaaagaa aactgaaaat caccatttac
    16741 cagtaatgtt ctcccacttc tttccaattg gacattttgt cccatgatgg gatagcggct
    16801 gtggagttca agagattttg gtactggaaa acggatttgt ttcatctgct gcggcttggt
    16861 tgtttctcct attttttgga gtcttctctc taatgttttc gcattctttt ggatggcttt
    16921 ttgtacactg tctttttgtt tagaggaaga caaccgatcc agtctgattt gttgcttttt
    16981 ctttttactg gacacttttt ccaacttttc cgcttgtttt ttcttttctt ggatcgattt
    17041 ttccaatcgt tttttctctt tcatgatcga ctctgcttct cgtagctgag tctttttctc
    17101 caattccttt tgagtgagat aggcactata attccccgaa aactctcgga tttctccgtc
    17161 ttttacttcc cagattttcg atgctagctc atctagaaaa tagcgatcat gactaacaaa
    17221 aaccagcgta ccataataat aacgaagctc ttcaatcagt cgatcaatac tttttctatc
    17281 tagatgggtt gttggctcgt ctagaagcat cccttgttca taagaagaaa gaagacgtat
    17341 caactcgatc tttcgttctt cccctccgct ccatttccgc tctgaagttt cttccagtct
    17401 taactggaaa tgactggcta attccaaatt taggtccttt gcttcagcga gataggttaa
    17461 ttgaggcagg tagttgaagg tgatctctgt ttggattttc ccatgatcgg gaaaaagttc
    17521 accggctatt agtttgagca aagtcgattt accgacacca ttttctccta tgatccctat
    17581 acgatcttgc tgatagacac gaagagaatc aatagatagt tctagctggt tgccaaagct
    17641 gacttgtaga tttgttatat ttactgctaa attttccata tgttttacac tcctatttca
    17701 tgtaaaacaa caattatcga tactaaaaaa gagcagaaaa atctgtttgc aaaaaaactg
    17761 catcacatct tttcctactc tgaacgaaat aactatgact aaatcaccaa atctgtcata
    17821 gctttcacct attctactgt cgttcgaaaa aatggacata ctccatccat gaaattgttg
    17881 tttatacatg tcttttgaat ttatttagtt gttattcaaa agttcgaaac gtaatttcat
    17941 cgatgcagtc atttttccga cctccttttt tattaggtta ccacacacga ttcaaaaaaa
    18001 gcaatagcta ctgaatagta actgaaaacc ttttattcga acatagtgat cttttctggt
    18061 gctaaacgaa cagactcgta accttcttct accgcttttc ctattgataa aataataact
    18121 ggaacataac ggtctttatc caaaccaaat gcctctgcca actgatctgc ttcgaatcca
    18181 ccaattggat ttgtttcata gccatgatca cgcgcaacta acatgaattg catagccgct
    18241 aagctagaat cgactttgac tacatcgttc atttcttctc ttgaaaaact cttgtaataa
    18301 ggaatgatcg cagctaattg ttgatctctg acttcttttg gcatcttccc tgattcgtaa
    18361 gcttggttgt agatttcttc gccatattcg taacattgca tatcgccaaa aatcatgacc
    18421 attgcagaag aagtatcatt ttgtctcgta ttgaaacgga tcaaaggacg caagatttct
    18481 tttgcttcct cgctttcagc gaccacaaag cgccatggtt tcatattgac agaagaaggt
    18541 gcagtcgtcg cttctttgat catttccagc atttcttcgt gtgggatttt gtatgtttca
    18601 tcatatacgc gaacggatct tctaccagtc ataattgttg aaaaatcatt tttttgttcc
    18661 attattatta acctctttcg tttctgttgt tatattgcaa agttgggtat tcaatttatc
    18721 taaaagtgtt agaaattgtt tcatttctac atcgctgaaa gccggggata gcacggaacc
    18781 tgtatcttta tggtccattt cacaatgctt gattttcttc tgggcttttt ctgttagcgc
    18841 tacatacact tcccggttgt tctgaggatt tctctgacgc gaaacatagc catttgcttc
    18901 caataccttc agatgacgag taactgctcc ttgatcaatc tgcaactttt cttgcaaact
    18961 gctttgtagc aatggatttt cttctttcag tctcatcatg atctcgtatc tagtaagact
    19021 tataccgata ttttcttcaa agatctggac gattttttga tcggctaatt tgatttgata
    19081 taaaaggtga ctaatctctt ctaaagacat aatttcctcc tttctaataa ttgactcgtc
    19141 aacgattgac ttgtcaataa tagaataaaa aaagaaaaaa gtcaaagaat cagctgtgta
    19201 ataaaaaaaa cgctctcaat agaaaagtat agagaaaaga aattttttgt agaatgaacg
    19261 gaaaaaatga taaagaaagc acaacgctaa aaccaagatt attgattctt atcaaactat
    19321 agaagatagc tcttctgcag tagataattt tacaccgcca aaaatttccg aaatcgcctt
    19381 ttgcaagaat cccgtcgaat attagaaatt ggatcagtat gcttaaacag taataaaaaa
    19441 gttatgacaa gatgttgtca caaccttttt tgagtaaacg gtgttcaaaa acttgcatct
    19501 gcggtaataa gttcaaccaa acaaaaaaca tgaaaatctg tttttgtttg gctgttctta
    19561 ctacttgatg cagggcagct ttttcactac ctctttttaa taaccataaa atcctattcc
    19621 ttcatatctc cagcccatcg gtgttagcca atctctttcc ttagcactca aggtatagtg
    19681 atgggtacca gttttttcat ttggattaaa tagtcgataa attggcttgg aaccaccaga
    19741 ataaaaactt agaccttcat aacgccaacc gatttttgtt aaagagttga tctcagattg
    19801 tgccattgtg taatgatggt ctccactgta aggattgtat aaacggtaga caggttgtcc
    19861 gctgtttggt gcttgccaag caatcccttc atattcccag cctacagttg gcagataatt
    19921 tttttctccg gcattcaatg tatataaatg ttctcctgta tgacggttat acagacgata
    19981 catcggagaa gttgaagttg gtgctgtatc atactgtgtc agattatagg tttcgattac
    20041 attgttcaat gtagaaccat aattaggcgc agtggcataa cgacctgtta gccaagccgt
    20101 agcatctttg taagagttcg tattgctttt ccatgcaccg gaatagtagt aaacgcctgg
    20161 ctgaaaagaa gtcgtcttca ataccgttac gttatcttgc aatgattctt tgtacgaagg
    20221 atattttcta aaagcggcat tcaccgtaat ccattgccca ttcacatatt ccgaagtagg
    20281 catcgtaaca gattggccat tatagcttcc tttaatccca aatagattgt aattaggggc
    20341 agaagccaaa gtactgttcc cccagccact ttcaacgatg gcttgagcaa tcatgactga
    20401 tgcatataag tcattggcac ttgccaaact ttgtgcagtt gaggcaatac tatcaatgaa
    20461 cgcttgttga gaattagcag atgagcgagc ataaaaagca tagccagtat agttttcgaa
    20521 ttcagacaaa tctgcttgat gggtatgtgt caatgtttgc ccatctgtca attggttcgt
    20581 gatttcttct gcattctctg gattgttctg agcttctgct gatgtactgt cttcggtttg
    20641 tgtttgttct gttgtttcag ttgttggttc ttcggttgaa attgtatttt ctgagacagt
    20701 agctgactgc aaagtacttt ctgtatcgtt ttcaattcct gtttctgaag aactcgatgt
    20761 ctgagttgcg acagtttctg tcgattcttc agctagcact gttgtgttgg caactgctag
    20821 atttcctaaa agaataacgc taaataataa attctttgct ttcatctttt ttctcctttg
    20881 aattatgaaa cttttcctaa ataacagtgt aactaaaaaa gtaaaagcaa gtcaatgtcc
    20941 atatttaaat aacatttgtt tctttttaat aaatttgctt tttctgcata aaaagacgta
    21001 aatgaagaat attcttctcc attcacgcct taaattgatg gtcgtttgaa taactattta
    21061 acaagtaatt ctctctggat attatccgtt atggattata cttcctgtgt cgtcggacgg
    21121 ataatcatgt tgctgacaga catccggtct ggcgtttcta tcgcaaatgc tacagcagaa
    21181 gctatatctt cagatgttaa tccccattct ttttgcgctt catgaagttc aagagcagcc
    21241 tttttgtcgc tgatggtttg atataattca gtttgaacag ctcctggaga gatgatcgta
    21301 gatttgatgt tattttgccg ttgctcttga cgtaatcctt ccatgattgc acggacagca
    21361 aattttgttc cgcaataaac ggcggaatca ggataaacaa catgtcccgc cacagaatca
    21421 gttgaaatga tatgcccaga tttttgtttt tccatgattg gaagtacggc ggaaattcca
    21481 tttaacacac ccatgatgtt gatatcaagc atattttgcc attcatcacg gtgaccttca
    21541 actaatggag cggttggcat gattcctgca ttattgtaca atacatcgat tcgaccgaac
    21601 ttttccatcg ctaaatcgat aactgcttgg acttgtgcaa aatctcttac atctgctgtt
    21661 tgaatatata gctcagcttc tggtaaagat tcttttattg cttttaggcg atcttctcgg
    21721 cgtgcagcta taactagttt agcccctttt tctgctaata gcttcgttgt tgcttcaccg
    21781 attccgcttg aagcgcccat gatgacaatc actttttctg ataaatcatt cattctattc
    21841 tcctcctgtt ttgaaaaatc tcttgcttat actcactatc tcttatttca tcttactgtt
    21901 caaataatcc gttcgacagg tttgacgatt ttggcaggga ttcctgcaac gatggtattg
    21961 tctgggacat ctttcgtaac agttgcattc gctgcaacaa tggcattctc gccaatcgtt
    22021 acgcctggca gaatagttgc tcctgctccg atccaagcat ttttcttgat gtggattggc
    22081 tttactgaca agcctcttcg ttcttttgga tcggttagat ggttcaccgt gatgaggttg
    22141 acacgaggac caatcaatac tgaatcttct aaacagatgc cacctaagtc aacgaacatc
    22201 acttgacgat tgataaagac atttttccct atactaatat gtttgccgaa atctgtataa
    22261 aaaggcagca gaacagtcac cgtatcatct actttgtcac taatgatatt ttctagccgt
    22321 ttgcggatat ctgctggttc atggtaccca gtattcagcg ctgctaaagg ctcttcgttt
    22381 tccgcttgga tctgatggat cacttcaaag agcggatccg ttactcgaat ctctttattg
    22441 acgatttgtt tttcgatgag gcttgtcatt ttgctccctc cttttatcct tactttataa
    22501 cggacggctt atgatgtcta atacttataa aacatactta actattcctt ttaactatac
    22561 ttagtggtat gcttttttaa aaacaggagg gaaaaaatgg atattcgtat actgacgtat
    22621 tttttagcag tagctagaga aaaaaatatc agccgagcag caaaatctct gcatatcacg
    22681 caacctactc tctcaaaaca actgaaagat ttagaagaag aacttggtgt gacacttttc
    22741 actcgaggct cccgagagat acagttgacg gattctggtc gatatcttta ttcaaaagga
    22801 aaagaaatct tgtcgttggt agaaaaaacg gcgaacaatt taacaaatga cgcaatcatc
    22861 tctggagaaa tctacattgg cggaggcgaa actaaagcaa tctctttcat ttctgatagc
    22921 cttcattcgt tgatcaaggc gcatcctgat atccagtttc atttatatag cgggaatgca
    22981 gatgatatca tagacaaact ggatagcgga ttacttgatt ttggactcgt aatcgaaccg
    23041 acaaacaaac aaaaatacca gtcatttcga ctgccagccc aagatcgttg gggacttttg
    23101 atacatcgga atcatccttt agcaaagcaa acagtcatcc atccgcagga actgaaaact
    23161 gttcctttga tcatttcccg gcaaacgtca gtggacagtc aactagctga atggctaggt
    23221 ttcagcatag atcagttatc gatcgtcgga acatataact tattgtacaa tgcttctttg
    23281 atggtcgata acgggacttt tggtgcatta gctctagatg ggatcatcaa tacttatggg
    23341 acggaaaaag tattcatccc actcgatccg ccattacaag cttcactgaa tctcatttgg
    23401 aaaaaagacc agcctctttc tcctgctgct cgtaaatttt tagatgtcct aacggagaaa
    23461 tacagttaag tagagttttt ttccttcgct ttcagctttt taacaacgaa taagttaagc
    23521 aaaagatcca atgattaaat aacataagtt tcttaatttg ttccttcctt ttatttctga
    23581 taattccagt aaattactat aattataaaa aaagaattga caaaacataa ctgtctatat
    23641 aaaattgatt acgttaacaa taattcgttt catgtgacta gatcatacta ggcatgaaaa
    23701 atctctgagg aacatggttt ccctaggagg tttttcgtgt ctatttttgt ttggccgcaa
    23761 tcaactggtc tagcaaacgt gaaaaccaaa tcttcaaaaa gggtgcgcgc atgaaaatca
    23821 aaaagatctt taatcaaaat gcagtattgg tagatgacgg tggacaggaa aaagtagcta
    23881 ttggaaaagg gattggattt gacagaaaaa gaaatgattt gatttttgat cgtgatgtcg
    23941 aacggatatt tgtcatggaa ccagaaggtc aaatcaagtt gcaaaattta cttaatcaga
    24001 ttgatgaacg ctttttattt gcagcagaaa cgatcattga tcatgcggaa actgttttga
    24061 tggaaaagtt gaatgaacat gtattgatcg ctttgaccga ccacattgct ttttctgcgg
    24121 agaacattaa taacggtatc gtcatccgca acaaactttt aagagaaatc gaagtgcttt
    24181 acagtgatga attcagtatt gctcaatggg cagtagagta tctgacaaaa gagcttggga
    24241 ttccatatac ttatgacgaa gcaggatata tcgcaatcca tattcacagc ggacgaagcg
    24301 gtcggaacaa taaccatcgc agtatccgtg aagtgacgat tgtatctgac atcatccatt
    24361 tagtggaatc agaattggaa acagatatcc attcagaacc attttcactg aattattcaa
    24421 gactggtgaa tcatttacga ctgcttctac aacgaaccca tgcgcaacaa tatgctgttt
    24481 tagacacgga aatcgtagaa atggtcaaac gaaaataccc agaaagctat aagatttcta
    24541 aaaaaatccg tgtgttgctg accaaagaat accagctcgc gatcacgaaa gaagaactgg
    24601 gttacttggc catccatatc gagcgtttaa gaagcgcaat cgtacaaaca aatgttaaca
    24661 atcatgagga ggaaaaaaat tgaaagcata tctacaacga atgggacgtt cccttcaact
    24721 tccagttgcg gtcttacctg ctgctgcttt actagtaggg atcggaaact ggtgggcatc
    24781 ttatagtaac gacattgtcg ctcatttttt acaagctggc ggtaacgcag ttttaggtca
    24841 attacctttg ctgtttgccg ttgggttagc actagggatg tcagtggaca aatcaggtgc
    24901 tgctgccttg gcaggtttgg tcgcatttga agttccagta aatgttttaa aaactgattc
    24961 agtagctact ttattgaata tcaaaccaga atcagttgat ccatcttttg ctcagatcag
    25021 taacgtattt attggtatta ttgccggatt gattgcggca gctctttata atcgtttcca
    25081 cgaagcaaaa ttaccgatgg ctttatcttt ctttagcggc aagcgtctag tgccaatctt
    25141 ggctgccttt gtcatgttgc tgatcagtgc agtgctactg ctcgtatggc ctccggttta
    25201 ctctgcttta gtttcttttg ctaaattcat tttgagttta ggctggggtg gtgcaggtct
    25261 ttatggcttc ttcaaccgtt tactgatccc tacaggattg catcaagcaa tgaactccgt
    25321 gttctggttt gacgtagcag gaatcaatga tattggtaaa tttttggctt ctgaaggaac
    25381 aaaaggcgta acaggaatgt atcaagctgg atttttccca atcatgatgt tcgggcttcc
    25441 agcaggagca tatgctattt acagaaatgc acgtccagaa aacaaagcaa aaacagcttc
    25501 cttgatgtta gcagcagctt ttgcttcatt ctttactggt gtaacagaac cacttgaatt
    25561 ctcatttatg ttcgttgctt ggccattata tgtcttgcat gcaattttca ctgggatttc
    25621 tttagcagtg agtgcattct tccattggac agcaggattt gcctttagtg ccggatttgt
    25681 cgattacttc ctatctttga aaaatccgat tgcgaatcag ccattgatgt tgatcgtcca
    25741 aggattagta acagcagtga tttactactt tggctttaac tttgctatca aaaaattcca
    25801 tctgatgact cctggccgcg aggaagcaga tctttcagat gacgatactg caacgactaa
    25861 ttcagacaat aaatatgcgg cacaagcaag caaaatctac gctgcattag gcggagccga
    25921 caatgtaact tcgatcgata actgtactac acgccttcgt ttgcaagtca aagatacagg
    25981 cctgatcgat caaaataaaa tcaaagccac tggcgtacct ggtatgaaga tcatcgacgg
    26041 aaaaaatgcg caagtcatcg taggtacaga agtacaattc gttgcagatg agatggcaaa
    26101 actacatggt ggtgctgcaa cacgacctgc acaaacaaac accgtggtta aaacagaaac
    26161 atttgccggt ggaacgatcg aacaagatat ttacgctatc gcaaatggga aactgattcc
    26221 aatcactgaa gtttcagatg atgtattcgc ggaaaaaatg atgggtgacg gctatgctgt
    26281 attaccagaa aatggagaaa tcttctctcc aatcgctggt acgatcacaa acattttccc
    26341 aacgaaacat gctgtcggaa tccaaacaga tgctggtatc gaagtcttgc ttcatatggg
    26401 catcaacact gttgacttaa aaggcgaacc attcacttta tatgtaaaag aaggtcaaaa
    26461 agtcgcaaga ggtcaattga ttgctttagt tgatcttgcc gctattcaat ctgcaggcaa
    26521 aaacacggat atggtcgttg tattcacaaa tggcgacaaa gtccaaagct tagaaatcga
    26581 acccgcacgt gatgtaaaag caaatgataa aatcggctct gtttccaaca aagcctgagc
    26641 catcgtcttc cccagacttt tttatcgata aacaaacagg agctgagaca aaaatcttgt
    26701 ctcagctcct ttttccgaat aaaaacggcg ttctcctgcc aactccgcgg taataagtcc
    26761 agccaaacgt aacatatgaa gagctatttt ttatttgact cttcttatta cttgatgcag
    26821 aacagctttt ttacaacctc tgataaacgg tgttcaaaaa ccagtttatc ggtatgaagt
    26881 caaatttatg caaaaatgtg gaaagccatt ttcacaaaat ttttcttcat actcaaaact
    26941 aatcggcttt ttcacaacct ctttatacat tattcctgat ccaatatttc ttttaatgct
    27001 gcttcaagag aagttaatgg ttttcccaat actttttcta aatcatcaga ggaaatagct
    27061 aactgatcct ttttaatatc atgttggatc gctaaaaaca tgtcagctgc ttcttgaggg
    27121 aatccggctt cttttagatt ttcgacaaac gctgcatcac ttgcttcaat aatttctaac
    27181 tttttacctg tcgcacgctc taatgccttg gctaattctt catagtcagt tagattgccg
    27241 gataattcta agatttctgg agagtcactt tctactagta cttttgctgc agcttctgca
    27301 tattcacgtt tcaatgccca acctgttttt ccatttcctg cagcataaac gaattgacca
    27361 gtttttaaag cctgcccaat gataggtaat tcattctcta cataccaatt attccgtaaa
    27421 aatgtatggg aaatacctga atccttaatc attttttcag tgaattggtg atctggcgct
    27481 aatacactga tcgagtgatc ggcatctgca aaacttgtat aagcgatata agatacccca
    27541 gctgcttgtg ctgccttcac tacattctta tgttcggctt gacgatttcc tggaacgcca
    27601 gagacaaaaa gcaatcgatc tattcctttc aacgcttctt tcattgagtc taaatcatca
    27661 taatcaccaa tacgtatatt aaatccagct tctttcagtg cttctccttt ttcttcacta
    27721 cgtactagta catagatttc attttgtgga accatctttt ttaagaattc cagcgcataa
    27781 ccaccaaaac ctccagttgc tcctgttact aaataatgca tatttgccac tccctattct
    27841 ttttatttgt taagatttac agctgtacat ttataaatta cacctttatc ataaagagtg
    27901 tggtaagata agtcaagcaa aaaaaatgaa aggatgacaa ctgatgaagt attctctgca
    27961 atttagtgat gcaatccatg tacttgccta tattgaaatg tttcaagata cccgatcact
    28021 ttctagtgag atgattgcca gtagtatcga aacaaatgca gcaaatgtac gtaaaatcat
    28081 gagtcagctc aaaaaatccg gtttgatctt tacaaaaacc ggaaaaccgc agccttccct
    28141 tgccaaaagt ccagatcaga tttctttact agatgtctat cgcagcatcg aaggtaatac
    28201 acagcttatc caagtcgatc caaaaacaaa tccaaattgc gtcgttggtg cgaatatcca
    28261 agaagcttta gaagaaagct acgctcggct gcagaaaaag gcagaagaag aaatggctaa
    28321 gatcacactt gatagtctga tacataaaat agccgttttg gaaaaagaaa aaagaccaga
    28381 aaacacaggc ctcgtagaga gatttttata attctctcta ctgtgcctgc atttttctga
    28441 tcggaatcgt ctacataaat tcttttattc tccgggggac aaaagccttg tcatgcccct
    28501 ttttcctctc taatgctcag gtcaaagcgc tgaagtcatg acgtcttgat aaacggtgtt
    28561 caagtagctg accctcggca ttagttctga aaaatgcaaa atatggaaag ctatttccca
    28621 tttttcctct ctaatgctca ggtcaaagcg ctactttcac gacctctata ttatcatggt
    28681 ttattgacta ccggtatcca gatttctccc atatatttcc cgttggtaac tcccatttcg
    28741 actacagcat ttggaccccc aacataggcg taatcttcta cttctcttaa aacatgccca
    28801 aaggccgtat tcgtcaaatc atttcttaat tcttcaggcg attctgctaa agcgattaca
    28861 acaagatacg ttccttttgg gaatgggatg attcgggtag tgtttggaac atcctgactc
    28921 gtttgaacac cgacatagta catcattttc ccgttatagg gttcgttgac tgtaaagaaa
    28981 taatgatttt cagcagcttg tttcaatgca tctattgttc cattaccgat aactttttgc
    29041 tcaaatgctt gtttctctgc agccaaaccg acaaaatccg tattgctact cttcaattcc
    29101 acaccaatac ccgtcaccgt aaaatctggt ttttcttcga tcctatactt tgccatttct
    29161 cccacccttt ctattatatt aagttcatct tacataaaat taaagggaaa tgacacgatt
    29221 acgctctttt ttctagggat atttttattt tttaactatt tttttgcttt cactgcaatc
    29281 caaatctcac tgtaaacatt actgaaatcc tctccccatt ttgtaaaaga gatctcggga
    29341 gcttcgacta actcataatc tgaagaagga agccattctg catagatttt cccccaagta
    29401 tcttgtaaag tttggggaaa tggcccttta ttaggaaaaa tggcccaagt gtgttcttct
    29461 atagcaagca tttctaaatc agcgtacgga ttttcttttg tcgtagcaaa gccaatcatg
    29521 tgcatcaaac tgcccttttc ttccaagcgg gctccatcaa atccataaga aacgttgatt
    29581 acttgatttg gatataaatc tcctaactta tgcatctctt ccctttgagt ctccgtgatc
    29641 gtctgcgcta acttttgtat ttcttgattg actccttcaa atttgacagg aacttttttc
    29701 gccacacctg ccacataaaa ctgttctttc ttttcgatac gaaattccat cgattttcct
    29761 cctctgacat caataaaaaa tgtcaagcgt ggaaaagatt tttgtatgcc gtatctgctg
    29821 atttctgaag gcagatatcc tgaccaatca cgaaaagctc ttgaaaaacc ttcgactgtt
    29881 tcatatccat atctaaacgc cgtttctgtc actcttgatc catctaggag atcgcgatta
    29941 gccaaagcca atctacggtt tttgatatag tccattagcg acatccctgc aataaacgta
    30001 aagatgcgtt ttaattgata ttcagagatt cctgtatttt cggctattat tttataggaa
    30061 agtccttctg tcaaatgttc ttcgatgtaa tcgatcaatt ggttcagatc tttaagcata
    30121 tttatcttcc tccttgcttg cctttttatc atattatcaa agaaagtcaa ttacttgatg
    30181 aatcaggaac caatttagtc agatgtagaa aatgttgtat ggatctaaag aaaattctca
    30241 agcagcgttt cgtaacggat aattggtatt atctgcctat ctatgtttta cctttagaaa
    30301 aataaatttt ggggaagaca cattctaagt aaacaaagcg gtaaaaatat cttttattcc
    30361 cttagttaat ccgcttacta cctactgtac aaagcaaatt gttgttcaaa ctgctgatca
    30421 gaacgctgct tggatttttc tttgcgatac atcatacctc ttttatcaat tggaacaatt
    30481 atttatttct aacaaaatca cttcattttt gtcagataaa taaaaaacaa aaaaagttcc
    30541 atctttaaaa cacgctattc ttttactttt tctcacattc tctaagaaaa gaaaaggaga
    30601 atgaattttt gacaattcga gcaaaagtga ttatcaaata tgtaaaatca gctacttttt
    30661 tcttaaacgt ttctttatac taaatataaa taagtgtttc atgaaaggag aactagatga
    30721 ataattggct tgagtatttt cctgaaaacg ttttggaacg cggttacagc tatcatctgc
    30781 acggttttgt ccgtcatttg aactatacaa gcaagtactt gtccgcaaca gtttctggca
    30841 cggaagatta taaggtagtg atcacttggg atgaaaagac caacatgact tgtgattgtc
    30901 tatatgccat agaaggaaaa aaatgcaaac acatggctgc tgtcttattc gcttacgaag
    30961 aacgtccaat aaaaaaatcc aactactcac tttccgaact ctcgtccctt gtttcctccg
    31021 ctagttcctc tcttgtacgg gagcttttaa cagagattct tatagaacat ccgcaattta
    31081 tcgaacgctt caaagttaag atgccatttc atgcaataaa ttattcagat aaattaacaa
    31141 ctatcatcca taaatacgat catattatca agaaaaacaa aaacaggaaa acagcgaagt
    31201 tcatcatgga gatgcgaaag ttcatccaag aagcagttga atcgctcatt cagcaaaatg
    31261 cctatcttcc cgcattcgaa ttgatcaatg aagtgattgc tactctcgaa acattctatt
    31321 gggagcctga ggatgaacga acccttcttc tgatagagga ctgttattat ttatggaaag
    31381 aattattagc agaagcacct catgcagaaa aaaggcaaat gttctcttgg ttcgtctgcc
    31441 aagtcgatca tactgacgcc tcttattcaa aacgctactc cataaaaatc ctgaaagaag
    31501 actttcggga aaaagaattt tctaaccaaa agaaaaaaat agataaaaaa actaaagaaa
    31561 cggtaaagaa agacgatttc aatgaaaaat agcaagtaaa cgataacaac catcctaaat
    31621 ccacaagaaa tacagcagac cttgctttaa aaacacgaat catgcaaagg atgaagtaaa
    31681 gaagacttaa cgcgtaccca atattagtca tttgactatt aggtacgcgt tttgttttat
    31741 aagtcatgac aaatttcctt cgttgctggg tagattttcc ggcattgttg atagatttta
    31801 tgatactttt ctacattttg taagagaggt tccactgcat ctgtatattg gataaaattc
    31861 tgacaacaac tttcaggtgt gtcgaaccac tctaatcttt aaagataaat attaaaataa
    31921 cgattaataa tattaacagt ctttattttt caccaccaaa aac
//
LOCUS       DAZBTF010000026        30947 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000026 DAZBTF010000000
VERSION     DAZBTF010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.026"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(296..1024)
                     /locus_tag="ACHGNS_001293"
     CDS             complement(296..1024)
                     /locus_tag="ACHGNS_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6937251.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="ACHGNS_001294"
     CDS             complement(1002..2576)
                     /locus_tag="ACHGNS_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFN6937252.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="ACHGNS_001295"
     CDS             2805..3743
                     /locus_tag="ACHGNS_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6937253.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="ACHGNS_001296"
     CDS             3755..4666
                     /locus_tag="ACHGNS_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6937254.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="ACHGNS_001297"
     CDS             4690..6162
                     /locus_tag="ACHGNS_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFN6937255.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="ACHGNS_001298"
     CDS             6213..6824
                     /locus_tag="ACHGNS_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFN6937256.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="ACHGNS_001299"
     CDS             6854..8767
                     /locus_tag="ACHGNS_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HFN6937257.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="ACHGNS_001300"
     CDS             8895..11876
                     /locus_tag="ACHGNS_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFN6937258.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="ACHGNS_001301"
     CDS             11873..13534
                     /locus_tag="ACHGNS_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HFN6937259.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="ACHGNS_001302"
     CDS             13574..14506
                     /locus_tag="ACHGNS_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFN6937260.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
     gene            complement(14898..16445)
                     /locus_tag="ACHGNS_001303"
     CDS             complement(14898..16445)
                     /locus_tag="ACHGNS_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFN6937261.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(16547..16900)
                     /gene="tnpB"
                     /locus_tag="ACHGNS_001304"
     CDS             complement(16547..16900)
                     /gene="tnpB"
                     /locus_tag="ACHGNS_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFN6937262.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(16890..17084)
                     /locus_tag="ACHGNS_001305"
     CDS             complement(16890..17084)
                     /locus_tag="ACHGNS_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937263.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            complement(17310..18512)
                     /locus_tag="ACHGNS_001306"
     CDS             complement(17310..18512)
                     /locus_tag="ACHGNS_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN6937264.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(18515..18709)
                     /locus_tag="ACHGNS_001307"
     CDS             complement(18515..18709)
                     /locus_tag="ACHGNS_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFN6937265.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(18756..19604)
                     /locus_tag="ACHGNS_001308"
     CDS             complement(18756..19604)
                     /locus_tag="ACHGNS_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HFN6937266.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(19809..20159)
                     /locus_tag="ACHGNS_001309"
     CDS             complement(19809..20159)
                     /locus_tag="ACHGNS_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937267.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            20337..21200
                     /locus_tag="ACHGNS_001310"
     CDS             20337..21200
                     /locus_tag="ACHGNS_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN6937268.1"
                     /translation="MFGLVQPNKVKVGQRIKEIKDGMSLSFTELGNRLGLKKPTISSY
                     VQGYALAPKSVINQLSSISGKPVGWFYFGHVEEYIREYLFLIGQKKIVDDYPEIIEKI
                     KQEFYTGEFKNPAWENELGYPLEEFIQECFVEYSSEIMKDYVVQITKEVLEESKTLNK
                     LSSKAKEEAVTILSSGIIDYIEMSGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSY
                     LIGKLINILGTDEDTELFLSSLSMIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREK
                     TPDEFYDWFEK"
     gene            21824..22816
                     /locus_tag="ACHGNS_001311"
     CDS             21824..22816
                     /locus_tag="ACHGNS_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6937269.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            22832..23716
                     /locus_tag="ACHGNS_001312"
     CDS             22832..23716
                     /locus_tag="ACHGNS_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6937270.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            23765..25372
                     /locus_tag="ACHGNS_001313"
     CDS             23765..25372
                     /locus_tag="ACHGNS_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN6937271.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            25373..26857
                     /locus_tag="ACHGNS_001314"
     CDS             25373..26857
                     /locus_tag="ACHGNS_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFN6937272.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            27034..28026
                     /locus_tag="ACHGNS_001315"
     CDS             27034..28026
                     /locus_tag="ACHGNS_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN6937273.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            28078..28947
                     /locus_tag="ACHGNS_001316"
     CDS             28078..28947
                     /locus_tag="ACHGNS_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFN6937274.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(29367..30932)
                     /gene="guaA"
                     /locus_tag="ACHGNS_001317"
     CDS             complement(29367..30932)
                     /gene="guaA"
                     /locus_tag="ACHGNS_001317"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFN6937275.1"
                     /translation="MSSPTRFDEEPKIIVLDYGSQYNQLITRRIREFGVFSELMSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGK
                     VKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCP
                     IASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGK
                     FGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
BASE COUNT         9705 a         5152 c         6244 g         9846 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagttgaaa
    14821 agtaagcgcc ccatagaaca gtacctgtac gtcaaataac ccgacctata gaatagaatc
    14881 gggaatgttt ggcgttttta gttacgagaa aaatgttgtt gtacatcctt gttccaaggc
    14941 aacagttgat ctaaagtttc tgctgaaagt acgggaagat ttggtatctt atcaaataga
    15001 aactctagat actttacagg attcaatccg ttaagttctg ctgttttata tacactcaga
    15061 atgattcctg aagttcgtgc tccttcaaga cttgtggaat gcaaccaatt tttccgtcct
    15121 atcaccagct ctttgatatt tcgctccgca cgattatttg tgagttctaa acgaccatct
    15181 tcaaaaatag gttcaaaacc acttcgtagt ttacacgcat attgaacggc tgcatccaac
    15241 ttggattttg caacaggatc tatcgttgcc atccactcat aaaaagcaat aaataatgga
    15301 cgcagttctt gttgccgaca gcttaatcgc ttctcaggag aaaagtcttt ccatttcttt
    15361 tctaggacaa accatttatc caattgtttg actgcttgag ctgcgggaat atccgtgttt
    15421 ttctttcctt ttggaattgc ttcgaaaaat ttccgccgaa catgcgcgaa acaatagaca
    15481 agtcgtacac tttccacggc attatacccg ctatagccgt cacagtgtaa ataccctgta
    15541 aactccttga gaaaagtttt cggaacatct ccagcacgac tttcgtcgtg gtgataataa
    15601 acgatgggat gctgactgtc ttttcctgta caaaactgcc agtaataggt atctgttttg
    15661 gcactttcaa tcacacgata acttgtttca tccccgtgta gaatctcttc ttgattaaga
    15721 tagactctta atcgttctcc taaagggtct aacgcgtttt gcactgcttt aatatgccaa
    15781 ttggtgatat tatctctcga aatatccaac ccatgtgttt gtttccaata gttctcttgc
    15841 cgataggcag gaactttctg accaaatttc atccggatcg tttccgccac taaactattg
    15901 gaacctaagc tattagaaat caaaggttta ggaacgatcg cttttttaaa agaagaaagt
    15961 ccttcttttt cgcagctttt acaacaatag gtatgttgat aatgggtgag acaatccaac
    16021 atagcctgat ggaaacgaat ttcttctcgt acttttgtcc gtccgatatc tttcatctgt
    16081 gaaccgcaat gttcacaaaa ttgttcttcc aaaggaagga gatgttcttt ctctgttata
    16141 ggtaagtgtt tgattttgtc tgctttcgtt cctacaactc gttgatgtcc ttttactgga
    16201 ataatggtgg cagctgcttc tggaacactt acagaaattt cttcatcact aaaaagattc
    16261 aattgcccat tcgtttcctc aaagagagtc tctttagagc gtccaaataa tttgttggtt
    16321 aaataattga ccttttcatt caatagttgg atttcagttg taagattctg aatggtttgt
    16381 tgttgtgctt gattggtttt atgggcttct tgaagcaact gaagcaacgt atctgtagtt
    16441 tccaaaattt ttcaccacct tgaatcgaac tgattttgtt acagaatacc acaagaaacg
    16501 cacaagaaat aggggttcaa cacatttttt gtgttaaccc cccagcttaa aaaactcctt
    16561 tttttcctgg caaaatagct cgaggttgat cgatacttaa tccttctaac aaccatcgaa
    16621 cctgttggtt cgttaaggtt cgaacttcgt ctttcgtccg aggccatttt aacttgccgt
    16681 tttctatccg cttataacag agaagaaaac catcaccgtc ccaatagaga agtttgaaac
    16741 ggtcttgacg gttcccgcaa aataaaaaaa cggcatcttc gtaaagctcg agttgatact
    16801 cttgttgaat aagactggca agaccatcaa ttccttttcg caaatctgtt tttccgcaca
    16861 caatataaat atgtttggtt tgaagtaaat cataaagcat tggacaacag ttctttcaaa
    16921 atagcttgaa gaatataatt gttaataccg ttatagatca aaacttcaga accattctct
    16981 cttttgatgc gcatcgctaa cttggcagcc gcgggtttac ctttttctgg tgcactcttt
    17041 tcgtttccta attggacagg aacaatctca tttaatttat acatagaaaa cctccagact
    17101 tcttgatagc tcaagtatat cttgaggtgc atttgttagt aaggtactgt tctatggggc
    17161 gcttacgttg aaaaacgttg atttatcggc attccccatc catattatat ttacgtctta
    17221 ttcccactca atatgagtag cctcgtaaag aagctattat atcagtgctt tttcagccta
    17281 ttcctcattt tggtagggaa atggtagggt tagattttta ctgcttgatt tccattaaag
    17341 tttgcaactt tagattttgt tgcacaggta aatttaatgt ttcctgtcat ttgttctgca
    17401 attccttcat cattacggct ccatagatga gcgtagtgct ttaaagtgat ttcagggcta
    17461 gaatggccta accgttgcga cacgaccaga atgtctgcgt taaactcatt gattaggtag
    17521 ctaacatgag aatgacgtaa tccttttcct tgtattaggg gtaccttagc tagtttggca
    17581 taacgctgaa taattcgttg aacagttgaa cgatagagtg ggaggtcagt gtagctaaga
    17641 ataaattgtt ctacaccatg atttttttga ataactttcc acttttttag cacttcaatt
    17701 gtatcatcgt ccagagaaat gatccgcaag ccgctttcag tttttgtgta aggtttccga
    17761 ataaaatctt tttgatttgt catttccaag gtgtgatgaa ccctcaactt tttacgtttg
    17821 aagtccacgt cattccaatt aagtgctaat ccttcgctta cacgaacacc agtcatgtaa
    17881 tatagccaga tcattaaaaa acacatatgt tcgtagaaat catctttata aatttttgcc
    17941 aaaacttctt caaattcttc ttttgtccag taaggaacga ttgattttcc ttttggaatt
    18001 gcttttgttc ttttggaaat attctcattc agaaattgca gggtgaccgc atagtctaat
    18061 gatttacgaa acataccgta cataagtgaa ctgtatgctt gtgagtatcc gctatcgttc
    18121 aataaccaaa ttcgataatt ttcacaatct cgcacattga tttcacgaag cttcttatca
    18181 ccgaaaaatt cagtaatctg aatcaatcca cactcacgag aactccaagt acttttttgt
    18241 acgctagctt tgtagtgtgg aacgtaagac tgctccataa attctttgta agttaaatca
    18301 taattcgcaa atccatttac aaccatgtat tcatttttta tacgcatgac ttctttgtgt
    18361 gcttcacgag ctgatgagaa tcgttttcct agagaatctt ttgttccttt cttctggatc
    18421 ctctttccag tcacttgatc tgttcctaac tcaactagat aagaaaagcg accattttta
    18481 tcttgataca cacaagggta ttttgttttc atagttattc ctcctgtgaa agaggaacac
    18541 ctataatttc ttcaattact tgtcggggta ctaccccaac tcttttatta ttataatatg
    18601 gatatccctg atttaccatt cttgtttttg cttgtcggat tattgtggta gcttgatgtg
    18661 ttttaaatcc tagagcctct aaatcttttc gacaaattgt atttttcatt gtataaactc
    18721 actttcaaag aaaacactat cattgggtaa tagtgttatt tctttatttt tgtataatga
    18781 acggtacttt cttgaaaatc catcacttgt tcaggacttc ccaactcatt taaagctaaa
    18841 gcattgtaaa aaaatggacg tctaactata tattcgcttg tatatagtat atcttgattc
    18901 gtggaatcat agggtttctt taccatcttt ttggtgacag ttggtagttt gagattccgg
    18961 gacttgaaaa aagctttttt cttatgctct ttcaattcca gctctttatc gaaatatttg
    19021 ctgatatagc gcccgcgatt ttcgatactg tctacatcga tcttgttgat cttaacgaaa
    19081 ccctgacccc aaatcgtagt taaccggtta aatgagataa aggggaaatc aaacaagata
    19141 atatggtagt ggattgcacc gcgttgctgc ttttcccacg tggcgagata ctttaaattt
    19201 gcttttttag ttttatagag gtcataattc agtcgtttga taaatttttt aaattcatta
    19261 tttgcatact caatgtcttg aatgttttct ttgaaagtga gagttaaaaa tttcgtttga
    19321 ttgtcaaaat tcgtgtctac aaggcgggca atctcaaatc gcatactttg ataatgtttc
    19381 tgtttgcgtt ttaaactgtc atattgtcct tgagcggata attcatcata gttttttctt
    19441 ttttcaagag taatatcatt ttcataaatc caagctaatt tttgttttcg ttccagattt
    19501 acttcgtttt cgatcgtttt acgtttggga gtaatgattg cttcataatc ataaatttca
    19561 atatacgtgg gagtttcaat gattttctga ttataagctt gcaaaaatca tcactccttg
    19621 ttgcgtattc gattgtcaaa gaaccagtca ttttctcctg atgttgatct ataaatcaag
    19681 ttaaggggga aggcctgcgt agcagccctt ctagcctatg gggtgttggg cggtcttaac
    19741 ggacagaaac ggctctgtgg gcgctgcccc gccgtttcgt cctaaccacc caacactcca
    19801 tagtatagtt agttttgtgt agtggtacct tgttttctgg gaatcaaagt tgccgtatca
    19861 gcggttgccg ataacccata actgacaaat tgtccattcg agcgtcccca aggtacaatc
    19921 accaaattgg taaatgtaat tatggctaaa ccttgttggg taagttcttc cggcgtgatc
    19981 acaggatgtt cagatttgac tttgacactg attttttcgt agcgaagttt tggtaggata
    20041 cattcgtagc tccaacctag aatttcgccc tccttttgcc agcgagtgac attgacgact
    20101 tggtattctt tttcgagtgt ttcttttggt aaactgagat cggtaatttt tattggcatc
    20161 acaattctcc ttttttatac gaaagtttat acgatttgat taaaaaatat agatggccat
    20221 ctacgacttg tattatatac gagaacttat acgtagtcaa gagattttct aaaaaaatat
    20281 atacgatttt ttatacgagt gttataatga aaaaaatgat aaggaggaat ttgaatatgt
    20341 ttggtttagt acaacctaat aaagtgaagg tgggccaacg aatcaaagaa ataaaagacg
    20401 gaatgtctct ctcttttaca gaattaggga atcgattagg tttaaaaaag ccgacgatca
    20461 gttcctacgt tcaaggctat gcgttggctc ctaagagtgt gatcaaccaa ttatctagta
    20521 tcagcggaaa acctgtcggc tggttttatt ttgggcatgt ggaagaatat atccgagagt
    20581 atttgtttct cattggtcag aaaaaaatag tggacgatta tcctgaaatc attgagaaaa
    20641 taaagcaaga attttatacg ggcgagttca aaaatcctgc ttgggagaat gagctaggct
    20701 atcctttgga ggaatttatc caagaatgtt ttgtagaata ttcgagtgaa atcatgaagg
    20761 actatgtcgt acagattacg aaagaggtac ttgaagaatc caaaacgcta aacaaactat
    20821 catcaaaagc aaaagaagaa gctgtcacga ttctttcatc tgggatcatt gactatatcg
    20881 agatgagtgg tgagatcaag tatggagaga aagatcgcat tgcgaaaatg gtgaaagaat
    20941 caataaaaac ctttgatgtg aaacaagacc tgacatttaa tgattcctat ttgattggaa
    21001 agttgatcaa tattcttggt acagatgaag atacggagct gtttctttct tctttatcaa
    21061 tgatatttac tgataagcat ttttccactc gatttggtgg ggaagaatta gtcgaaatat
    21121 ttcaatcgat gcgaccagcg ttaatcaaac tctatcggga gaaaacacca gatgaatttt
    21181 atgattggtt tgagaagtaa aataagaaaa gtggcttaga ttacaagttg gtgaaaaaat
    21241 ttttttcagc ataacatcag taatctataa aggggttgtt gattatactg tagaagttaa
    21301 gtactcaaga gattttttgt acgtaaacaa agcaaaaaag ggtgcagttt cactagaaaa
    21361 tgtactcttt tttgtgattt ttcttagtta tagagttttt ttgaaacttt aaaaatgaga
    21421 aaacatattg attttaaaga gtgtagacat agcttctttt gctaaataga ttaaacttac
    21481 tggtgagtgg tcttgaacat acttttaaat cacattttac tgagttttat tggattttat
    21541 aattcattgg aaacctcgta cttaatctgt ttttatctat cgactagaat ctgtatatat
    21601 tccagctatc aatgaggccg cagaaaaggg tgcttaaaaa taatctgttg tttaaaatat
    21661 tattatcaga atacttagat acaaaattct ggcacaaagt aaaaaaaata ttgacttaaa
    21721 aaaatggata tggtaatcta taaacgaaaa tagtggtaac gtttccaaaa tgcatcttcg
    21781 ttaaattttt atttttttaa acgaaaatgg aaacgtttct aaaatgtatt gggaggagat
    21841 tgaattggaa aagacattgg gaccaggtgg tgagataagg gcggtacaac gggataaaaa
    21901 aaatatttcg aagtgggaaa aattaaagaa acagttgcgg cgtgataagt ggatgtactt
    21961 gttgctctta ccaggtatca tcatgacgtt tatctttaag tatgtgccaa tgtacggtgc
    22021 tgtgattgca tttaaagact ataatcctat gctaggaatt ttggaaagtc cttgggtagg
    22081 actagatcac tttagggaat ttatcagttc accaaatttt acgcttttgc tgagcaacac
    22141 attaaaaatt agtatttatg gtttattgtt aggttttttt ccaccaatta ttttagcact
    22201 gagcttgaat caaattatca ataaaaaaat aaaacagaaa atccagttga ttctatatgc
    22261 gccgaatttt atctcagtag ttattattgt ggggatgctg tttttgttcc tatccgcaaa
    22321 tggacctatt aatgggttag tagaaaaggt tacggggcaa ccaattatgt tcatgtctaa
    22381 tccagcttat ttcagaacga tttatatttt atcgggaatt tggcaaggaa tcggctggtc
    22441 atccattctt tacaccgcaa cgctggcggg agtcagtacc gatttatatg aggcagcaaa
    22501 tatcgatggg gcaaatattt tacaaaagat ttggcatatc gatttgcctg cattgaggcc
    22561 tgtgatggtg atcagcttca tcttggccgc cggcggtatt atgaatgtag gatatgaaaa
    22621 agcctacttg atgcaaactt caatgaacat ccctgcctca gaaatcattt ccacttatgt
    22681 atacaaagtc ggcttgcaat caggtgatta tgcctactct gctgctgttg gtctgttcaa
    22741 tatgattatt aatgtcgttt tattactgat tgtcaacaat ataaccaagc ggctaaacga
    22801 tggtcaaggc ttgtaatgaa aggagaaaat tatgaatagt gctttatcaa aaatcgaccg
    22861 tcaaatctta tggttgaatc gagtgattgt aactttttta gtcgtcatca ccattgttcc
    22921 gctgatttat gtactggtag cttcttttat ggacccagct actttgatca ataaaggtat
    22981 aagcttcaat ccgaaagatt ggacaataca gggatatcaa cgggtatttg cagatgattc
    23041 cattttacgc ggttttgtaa attcgttgtt ttactctttt agcttcagct tattgactgt
    23101 atttattacg atgatcactg cgtatccttt gtctcgacct aacttggcgg ggaaaaagat
    23161 tattatgact ttttttgtca ttacaatgtt cgtgggtggt ggcttgatac ctacctattt
    23221 attggtaaag aacttgggca tgttagatag tgtatgggcg attatcgtac caggtgctat
    23281 aaatgtgtgg aatattatct tagcccgtac ctattttagt gctttgccaa aagaactttc
    23341 tgaagctgct actatcgatg gcgccaatga cttgcaaata ctgttcaaag tcatgcttcc
    23401 attagcgaaa ccaattatgt tcgtattgtt tttatatgcc tttgttgggc aatggaactc
    23461 atactttgat gccatgattt acatcaaaga tcccgactta cagccgttgc aactcgtatt
    23521 aagaaatatt ttgatccaaa accaaccttc acaagacatg gtgggagcca atactgccat
    23581 ggcagaaatg aaacaaattg ctgagttgat caaatacgca acgatcgtca tttctagtct
    23641 gccattaatc ataatgtatc ctttcttcca aaaatatttt gataaaggtg ttttagttgg
    23701 ttctgttaaa gggtaagtat tgttcactat ttgtatgaat aaaaattata aaggagattt
    23761 taagatgaaa gcaaaatgga tgctgccaat cgtttttact tccgtactaa ttttgggggc
    23821 ttgtggaggc tcaaaagaaa agagtaaatc aagccctgat tatgaactag aaaatgtctc
    23881 ttttccattg aaagaaaaag tatctttaaa aatgatgtct caaagtgccc ctttggctcc
    23941 caatgatcct aatgacaaat tgattttcca acgattagaa gaagagacca atctccatat
    24001 tgattggaca aactacagta gtgattttgc cgaaaaaaga aatttggata tcgctagtgg
    24061 tgatttacca gatgccatct tcaacgccgc tgcaggtgac tatgatttat taaactgggc
    24121 ggaaagtggc gttattgtac cgattgaaga tttgatcgaa gattatatgc caaatctgaa
    24181 gaaaattttt gatgaaaacc ccgaataccg acaaatgtct actgcacctg atggtcacat
    24241 ttactctatg ccttggattg aagaattagg cgaaggaaaa gagtcgattc atacagtaaa
    24301 tgggatggct tggatcaaca aggagtggtt ggataatttg ggtcttgaaa tgccgcaaac
    24361 cactgatcag ttgatgactg tgttggaggc tttcaagacg caagatccaa atggtaatgg
    24421 tgaagcagat gaaattcctt ttagctttat taacaatgga ggaaacgaag acttaaaatt
    24481 tttaatgggg gcattcggtc taggtgataa tgatgaccac ttagtcgttg ataacgatgg
    24541 tcaggtacaa tttactgcta acaaagaagg gtataaagaa gccatcaaat atttcaatga
    24601 aatgaataat aaaggtctta ttgataaaga ggctttcgaa caagattgga atacgtatgt
    24661 tgccaaaggg aaagaacata agtatggcgt gtactttact tgggataaag caaatatcac
    24721 gggtgcagat gatagttatg acgtcttgcc agtcttagcg ggaccagatg gaacgaaaca
    24781 tgttactcgt acgaatggca tgggtttctc aagagatcgt ttcgtaatta ctagtgcgaa
    24841 taaaaacttg gaacttacag ctaggtggat agataaaatg tatgaaccgt tacagtctgt
    24901 ccaaaataac tggggaacct acggtgatga agcacaggcg aatatttttg aattaaagga
    24961 tggtatgtta aaacatttgc ctttagaggg gacagcaccg ggggaactaa gacaaaaaac
    25021 cgaagctgga ggaccattag cagttcttga ttcttattac ggcacagtca ctactatgcc
    25081 agatgatgcg aagtggcgtt tagatttgat gcatgacacg tatttgccat atattacaga
    25141 agacaatatc tatccacgtg tctttttaga aaaagaagaa acagatcgat tagcaaaaat
    25201 cgaaacggat atgtgggatt acatctatcg caaaagagca gagtggatca ccggcggtaa
    25261 aatcgatgaa gaatgggaag aataccttgc tgaattggaa cgtgtaggtt tggaagaatg
    25321 gattcaaata aaacaaactg gctatgataa ttttttgaag ggagcacaat aagtgttagt
    25381 agaagaaacg gcgattttgg ataaagcaaa tcgatttatt gaaatgatgc gaggaaaagt
    25441 taaccctcag tatcgacaaa actatcattt ttccgcaccc atcgggtgga taaatgaccc
    25501 caatgggttt atatattaca aagggcatta ccatctcttc tatcaatact atccttacga
    25561 tagtcgatgg gggcccatgc attgggggca tgcgcgcagt agtgatttgg tccattggga
    25621 agatctacca gtagcgttag ccccagatca accttatgac agcggcgggt gtttctcagg
    25681 aagtgcaatt gaaagagacg gaaaactata tttattttat acaggctgtc aagaaattga
    25741 tggtattagc catcagcatc aatgtttggc ggtctctgaa gatggtattc attttagcaa
    25801 atacagtggt aatcctgtgg tagcagaaaa acagctaggt gaaaatgggc taatacaaga
    25861 atttcgtgat cctaaagtaa ttgttcacaa cgatggttac tatatggtag tagctactaa
    25921 atctacacag tctactggta gaatagtgat gtttcaatcg tcagatttgt atacttggca
    25981 cttttactct gtgctgctag aagggacttc agagcaagga ataatgtggg aatgtcctga
    26041 tttattccat ttagacggac gagatgtctt aatcatgtct ccgatacaaa tcaagaagaa
    26101 cggactggaa tatcataata tcagttctac catggcttgt attggtgagg taaactggga
    26161 aacaggtcag atgatagttg ataatttcca tgaaatggat tttggaatgg atttttatgc
    26221 accacaaaca acaagagatc ctaatggtgg acgggtgatg attgcttgga tgcagatgtg
    26281 ggatcgtaca ttgcctacac atgagttagg ccatctttgg gcaggctcta tgagtttgcc
    26341 ccgagagtta agagtgaaaa aaaatcgatt ggtacaaaaa cccatctcaa ctgtatatag
    26401 tcatttagtt tatcaacaag gcagtgaaaa tattgcagtg actgacacat cagtcgtatt
    26461 tcgaaacgcg ctgtctgatt cttcatattt acatctagtc gcagatttgg agaaagctga
    26521 agtttttaca attgaattac tgcggtctaa gacggattgt ctcaaactag cctatgacac
    26581 tgctacacag gtttttgaat tggatcgtca gaaactagga agagagctta caggggcgga
    26641 agaagaccct ctgaatgtac gaaaagttga agtacctttg attgataatc agctcgttct
    26701 tgagattttt agagatacct catcaattga agtctttatt aatgggttag aagttatgac
    26761 ggctactttc tatgagctgg aaaaagctcg agatatcttg tttcattcga tcggagaaag
    26821 tcgtattcaa gcttttgaaa cagcgagtgt gaaataaatg tttgattgct taggcgatga
    26881 atcaattatt taataggttt tgaaggataa tgtttttaga aaagaacaga tagctagatt
    26941 tcccatggat ttgctattag tattttgaaa aaattagtaa tttatgacaa aattcattat
    27001 actaaacttg tgcagaaatg tgagggatca actatgaaag caacaatgaa agatgtcgca
    27061 aaattagcag gtgttggagt aggaactgtt tcccgggtat tgaataacgg ttctgttaaa
    27121 gcttctacta gagaaaaagt tgaagcagca atgacagagt taaactatca accagatttt
    27181 tatgcccggg ggttaaagat gaagcatacg aacacggttg ctttgattct acccacggta
    27241 tggcatccat tttttggcga atttgcctac catgtagaaa cagcattaga aagacaaaac
    27301 tacaagcttt atttgtgtaa ctccagtggt aatagtgaaa aagaacaaga atatatccag
    27361 atggtcagcc aaaataaagt agacggcatc attggaatta cttattcaga tatcgatgaa
    27421 tacatttcga gtaatctgcc ttttgttagt attgaccgtt atttcactga agaggtgatt
    27481 tatgtgaccg ctgacaatgc ggccggtgga cgtttggctg caaatgaact ttatcgccga
    27541 ggatgtcaga atgtagcata tatcggtggg taccaagaaa caccgaatga aacgaagagt
    27601 cgtcgaaaat attttgaaat cgagtgccgt caactgggga tttcctataa agcgttagat
    27661 atgttagaac ctttagacca tctctcagaa cgtgtaacaa attttttgtt agagaatccc
    27721 gatatcgatg ggatttttac gatcaacgat ccaatggcat tagctgtcat tgatatgctt
    27781 gaaaaaatag ggaaaaacgt accagaggat gtccaagtta tcggatttga tggtcaaaag
    27841 atgacagaag gagatcctta tctagtctca acgattgccc aacctacaaa gcaaatggcg
    27901 gaagtggcag tggcaaaatt aattgctgtc attaatggag agacagtaga aaaacgtacc
    27961 gtattgccga ttgaatttgg cgaaggtgac acaacgaaac ctttagtgag aaacgatttt
    28021 gcttagtgca aatacgtttc tttttttgaa aatgagtgaa tgaaaggtgt agataaaatg
    28081 ttatatggtg gaatagaagc cggtggaaca aagtttgtct gtgggattgg taatgaacaa
    28141 ttacaagtga tagaacggaa aagttttcct accacaacac cagaagaaac gatggagcaa
    28201 gtgtttgatt tctttgataa gtacaaagga aatatcgcat caattggtgt aggagcattt
    28261 gggcctattg atattcaaat cgaatcaaga acttacggat atatcaccaa cacaccgaag
    28321 ttagcttggc aaaattatca ttttgtagga gctttggaaa acgccttgtc ggttcccatc
    28381 tattggacta ccgatgtaaa tgctgcttgt tatggagaat atatggcagg gtatggaaaa
    28441 ggtaaaaata gtgttctgta ttacaccgtt ggtacaggtg ttggtggtgg tgctgtagtg
    28501 gatgggaaaa tagtcgcagg ttttagtcat cctgaaatgg gacatatgct cttacaacgt
    28561 cataatgaag atcaatttga aggaatctgt ccttttcatc atgattgttt ggaagggtta
    28621 gcatcaggac ctgcgattga aaaacgcttt ggaagaaaag cccagactat ctcatttgat
    28681 catccttatt ggacgattga ggcagattat cttgctcagt gtgtgtataa cacaacgtta
    28741 ctatatgcac cggaagtaat aattttaggc gggggtgtaa tgaaacagcc gcaactactc
    28801 gagttgattc gccaatcgtt taaagaaaaa atgggttcct acgtggatac gcctactctc
    28861 gaagaatatc tactaactcc aaggcttgct gacaatgcgg gcttgattgg ctgcctagct
    28921 ttagcaataa agaaactcaa tcattagaat agtatttatt agaactattc ataacatacc
    28981 ggaagtttaa aagggtattc tgaaacttaa atagtctcaa ttcattcttc tttcttgaga
    29041 atctgagtag agacttttat tctgtttcaa attaatataa ttacgaaatg atattgagta
    29101 caagaaagtt ctttttacca gatcttttaa atcgaaaaat ctgatatata tatttatagt
    29161 actaaaaatc tgattagata aaaagctaat tcaataggaa aataaaggta gtgaataaaa
    29221 gcaaagccct tgttagtagt taatctaaca agggctttgg tagggaaaat ggtaggaaac
    29281 catcttattt tttacgaaaa tcaggaactt tagtaattga ataatgttga tttataggta
    29341 ttcctcatcc atattatgtt ggctctttat tcccactcca ctgttgcagg tggtttgctg
    29401 gtaatatcat acacgatacg gttaacgtgt gcgacttcat ttacgatacg gacactgatt
    29461 ttttgcaaga cttcccaagg gatacgggca aagtcagcgg tcatgccgtc aatagaagtg
    29521 acagcacgga tacctacagt gtagtcatac gtacggccat ctcccataac cccaacagag
    29581 cgaatgcctg gtaggacggt gaagtattgc cagatgtcgc gatctaaacc ggcattggcg
    29641 atttcttcgc gcaagatagc gtctgattca cggacgattt ctaatttttc ttctgtcaat
    29701 tcaccaagga cacgaatacc taagcctggt ccagggaatg gttggcgcca aacgatgctg
    29761 tcaggcatgc ctaattgggt acctaattca cgtacttcgt ctttgaacaa tgtgttcaat
    29821 ggctcgatca gcttgaattg catgtcttca ggtaatccac ctacattgtg atgtgatttg
    29881 atggtttggg cagtttcagt tcctgattcg atcacatcgg tataaagggt tccttgtgca
    29941 aggaaagaaa cgccttcttc accggcaagt ttggttgctt catcatcgaa gacatataca
    30001 aattcatttc caatgatttt acgtttttct tctggatcag acacgccagc aagttttgat
    30061 aagaaacgtt ctttggcatc gacttttata atgttcaagc caaattttcc gccaagactt
    30121 tccataactt gttctgcttc acctttacgc aacaatacgt gatcaacgaa gatacatgtt
    30181 aattgatcgc cgatcgcttt ttgtaaaaga acaccaacaa cagaggaatc tactccgcct
    30241 gaaaggccta ataaaacttt tttgtcaccg acttgttcac gaatttttgc gatctccatg
    30301 tcgatgaagt tatccattgt ccagtcacca gtacattggc agacatctag tgtaaaatgg
    30361 cgcaacaagt catttccata ttcggaatgg cgaacttctg catggaattg gattccgtac
    30421 attttgcgtt ctgcattttg gatcgatgcg atggggcaat ctttgctggt agcaacgaca
    30481 tcgaaatcag tcggtgcttg ggtaactagg tcaccatgac tcatccaaac ggtttgtttt
    30541 tcaggagtgt tttggaaaag ggccgcttct gcagaagtga tttccaattc tgcttttccg
    30601 tattcacggt ttttggctgg tttaactttt ccgcctaatt tataagtaat caactgcata
    30661 ccgtaacaga ttcctaaaac tgggatcccc aaatcaaaaa tcttttcatc aataccaaag
    30721 gccttctcat cataaacact gtttggtccg ccagaaagga tgattccttt agggttcatt
    30781 gcctttactt cttctgcggt aatgcggtgg ctcatcaact ctgagaaaac accgaactca
    30841 cgaatacggc gagtgatcag ttggttatac tgactaccgt aatcaagtac gataattttt
    30901 ggctcttcgt caaatcgtgt tggtgaagac aaatttgaat aaaactt
//
LOCUS       DAZBTF010000027        30911 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000027 DAZBTF010000000
VERSION     DAZBTF010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.027"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..253
                     /locus_tag="ACHGNS_001318"
     CDS             <1..253
                     /locus_tag="ACHGNS_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN6937276.1"
                     /translation="TEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVPHSNG
                     VTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            complement(351..1934)
                     /gene="abc-f"
                     /locus_tag="ACHGNS_001319"
     CDS             complement(351..1934)
                     /gene="abc-f"
                     /locus_tag="ACHGNS_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFN6937277.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIVLVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(2039..2167)
                     /locus_tag="ACHGNS_001320"
     CDS             complement(2039..2167)
                     /locus_tag="ACHGNS_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937278.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(2512..2655)
                     /locus_tag="ACHGNS_001321"
     CDS             complement(2512..2655)
                     /locus_tag="ACHGNS_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFN6937279.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(2878..3357)
                     /locus_tag="ACHGNS_001322"
     CDS             complement(2878..3357)
                     /locus_tag="ACHGNS_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFN6937280.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(3430..3714)
                     /locus_tag="ACHGNS_001323"
     CDS             complement(3430..3714)
                     /locus_tag="ACHGNS_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937281.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(3792..3986)
                     /gene="ssrS"
                     /locus_tag="ACHGNS_001324"
     ncRNA           complement(3792..3986)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHGNS_001324"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(4058..5344)
                     /locus_tag="ACHGNS_001325"
     CDS             complement(4058..5344)
                     /locus_tag="ACHGNS_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFN6937282.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            5482..6153
                     /locus_tag="ACHGNS_001326"
     CDS             5482..6153
                     /locus_tag="ACHGNS_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFN6937283.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            6119..6607
                     /locus_tag="ACHGNS_001327"
     CDS             6119..6607
                     /locus_tag="ACHGNS_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFN6937284.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            6620..7567
                     /gene="prmA"
                     /locus_tag="ACHGNS_001328"
     CDS             6620..7567
                     /gene="prmA"
                     /locus_tag="ACHGNS_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFN6937285.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            7568..8332
                     /locus_tag="ACHGNS_001329"
     CDS             7568..8332
                     /locus_tag="ACHGNS_001329"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFN6937286.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            8565..10778
                     /locus_tag="ACHGNS_001330"
     CDS             8565..10778
                     /locus_tag="ACHGNS_001330"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFN6937287.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            10789..11235
                     /gene="dtd"
                     /locus_tag="ACHGNS_001331"
     CDS             10789..11235
                     /gene="dtd"
                     /locus_tag="ACHGNS_001331"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFN6937288.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            11406..12665
                     /locus_tag="ACHGNS_001332"
     CDS             11406..12665
                     /locus_tag="ACHGNS_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFN6937289.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(12754..13701)
                     /locus_tag="ACHGNS_001333"
     CDS             complement(12754..13701)
                     /locus_tag="ACHGNS_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFN6937290.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            14095..15387
                     /gene="hisS"
                     /locus_tag="ACHGNS_001334"
     CDS             14095..15387
                     /gene="hisS"
                     /locus_tag="ACHGNS_001334"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFN6937291.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            15402..17171
                     /gene="aspS"
                     /locus_tag="ACHGNS_001335"
     CDS             15402..17171
                     /gene="aspS"
                     /locus_tag="ACHGNS_001335"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFN6937292.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            17280..18155
                     /locus_tag="ACHGNS_001336"
     CDS             17280..18155
                     /locus_tag="ACHGNS_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN6937293.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            18421..20304
                     /locus_tag="ACHGNS_001337"
     CDS             18421..20304
                     /locus_tag="ACHGNS_001337"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFN6937294.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(20379..20663)
                     /locus_tag="ACHGNS_001338"
     CDS             complement(20379..20663)
                     /locus_tag="ACHGNS_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFN6937295.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(20653..21387)
                     /locus_tag="ACHGNS_001339"
     CDS             complement(20653..21387)
                     /locus_tag="ACHGNS_001339"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFN6937296.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            21516..22139
                     /locus_tag="ACHGNS_001340"
     CDS             21516..22139
                     /locus_tag="ACHGNS_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFN6937297.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            22331..23452
                     /locus_tag="ACHGNS_001341"
     CDS             22331..23452
                     /locus_tag="ACHGNS_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFN6937298.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            23459..26587
                     /locus_tag="ACHGNS_001342"
     CDS             23459..26587
                     /locus_tag="ACHGNS_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN6937299.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            26606..29800
                     /locus_tag="ACHGNS_001343"
     CDS             26606..29800
                     /locus_tag="ACHGNS_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFN6937300.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(29946..30653)
                     /locus_tag="ACHGNS_001344"
     CDS             complement(29946..30653)
                     /locus_tag="ACHGNS_001344"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN6937301.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        10083 a         5637 c         6558 g         8633 t
ORIGIN      
        1 tacggaaggc tttcaagaaa cccttaaaac aatttcatct acgttacctc agtcgtttaa
       61 aaagaaattc acgatcttcc atcgctacca atcgggtatt tcaaatgcct ttaaggtgcc
      121 ccactcaaac ggcgtcactg aggggctgaa taacaagatc aagctgatta aacgaattgc
      181 gtttggttat cgcaactttt ataactttcg ggcacggatc tatttacaac aaggtctgat
      241 ctttgaaaac taaaaaaata tgcggcgcac agaacgcgcc gcatatcgga ctaagtttta
      301 ttcaaatttg tcttcgccaa cacaatttga cgaagagccg aaaaaacgtc ctagtcaatc
      361 agttggccga gcggctggcg gctgatctgc tcctgatcaa taacgagtgc ttcttggaag
      421 tgttgctgca aaaagtagcg gtcatggctg actgctagga gcgttccttg atagtcttgc
      481 agcatttctt caatttcttc tcgagcatag ctgtcgaggt gattggttgg ttcatccaaa
      541 atcaggacgt tgattttttc taaaaagagt ttcagcaacg ccaaacggat tttttcacca
      601 ccggataaat ctttgatccg tttacccaca tcgctttgat aaaagccact tttggctaat
      661 tgccgtcgag cgacttcttc gtttccagtc aatgtgatgg cgtaagccag caaacggcta
      721 tcgggatcgg caaactgcag ctgctgcggc aaataaccga tttgcaagct actacctaat
      781 ttgatggtgc caccgtaagg cttttcctca ccgagtaata atttgagtaa ggttgatttt
      841 cctgcaccat tgtcgccgat gatggctaaa tgatctccac gatagactgc aaaactgctg
      901 tctttgaata gtacttgatc agcgtactgc tggtgcaagt tttgaatgat gaaaacttct
      961 ttgccagatt ttccaccatt agacaagctt tgcagccgat ttttcggggg aggtggcggt
     1021 ttgacgagtg tcagtttcgc caggcggcgt tccagttctt tggctttttt gaagaaggac
     1081 tcattgtctc cttcatgtgc ccactgacga aaacgacgaa tcatgacttt caaccgctgg
     1141 acttcttttt gctgcagttc gtaatttttt gtcagctcag ccaagcgagc ttctttcaag
     1201 actcgaaaac ggctgtagtt tccgggatac tcaatcaatg caccttcttc cagttcccat
     1261 gtttttgtgg tgacttgatc gagaaattgc cgatcatgag aaacgaccag aaccgatttt
     1321 tttgtattct gtaaatagtt ttccaaccag cgaatactgt taagatctaa atgattggtc
     1381 ggttcatcga gtaaaagtaa atcggcatcg gctgctaaaa ttttggctaa agcgacttta
     1441 actcgttcgc ctccgctaag ttctgttaac cgtgcttcct gacggtgtgg aatccctaag
     1501 ccttgcaaga tcgctgcaat ccggtcttct aatacgtaac cgcctagttc ttcaaagcga
     1561 tgctgcagct caccgtaacg ggccagacac ttctctaagt ttgtatctgg ttctgtcatc
     1621 aatgcttcgt agcgactcat ttgctgtttg aggtcctgta tctcagcggc agaatggagc
     1681 aagtagtggt tgacggtcgc ttcggaaact gtaagttctt gttcgaccgt cccgatcttt
     1741 aagccttttt tgcgactgat aacaccgctg tcgacacctt ctctgccggt caagatttgt
     1801 aaaaaggttg atttgccagt tccgttgata ccgactaaga caattttttc accagcttca
     1861 atttgacagg ataaatcctc aaataatgga ttcccgccga ttgttttttt aatagaaatt
     1921 gcttgtatga tcatgatctc tctccttgag accactcaaa aaggccatgg ttatccatgg
     1981 cctgtgttat ttaaactcag attatcatga gaaaaatacc ttgagatggc cattgatttt
     2041 acgtgaattc aactgttttg gacaaacttg tcccgtcaac agcaactgta aaaccaattg
     2101 catggctaaa tgcgatatgg tatcgttggt aattttcatt acttgttatt ttagtcatgc
     2161 gcatcatagt attttccctc cttcgtgtag ttgtcttttc cagtgtacct gaaagtgtca
     2221 gaaagttcaa ctagcgctga catttactta atatttatcg atgtgtaatt ctgtcagaga
     2281 gcttttttta tctgactgag ggtaatgtct cgtggaccac atcattgtat tctgagctaa
     2341 accagcgttc aggcgaaatc atatggtgtt tgataaaaaa ctttgtctac atgacttcct
     2401 tttgccattt gcttaatacc gttgcattaa acagctcttc tctttggaaa tctcaccaac
     2461 atcgcgttca ggagtatcgt tgtggttgca atggcaagcg gtagcctttc atcaattctt
     2521 gaatgattta ccgaatgtag cacttgcctt ctcgcggtac ccgaacccga aataaaattt
     2581 tagtttggcg ataaaatcgg tatccgccat cgatacttca tattttttcg tatcagattc
     2641 atcggaaact tccacattga cgaaccaata agctttggca tgcttaggtc gttttaaacc
     2701 acaccagggc gactaatcgc cctggtgttc actttttttt attttacttg aactcattga
     2761 ctgttttccc tttattttaa acattataaa gaccgatgag tgcttgccgc gtcttttgtt
     2821 tttatcacgg ttttttgtgc aaaaaaacct gccgatagtt ggcaggtcgt ctacgtgtta
     2881 agctttggtg tggtctttag ctggcaattg gttttctaaa tcagttcgga caacaagcac
     2941 atcgcaagct gcgtttctaa taacatattc agaaacagaa ccaataaata aacgttctac
     3001 agcattcaat cctgtggctc ctaacataat caaatctact tgattatctt ctgggatttc
     3061 tctggcaata atttgttttg gtgagccata ttctacgaca gaggttacat tattcaagcc
     3121 agctttcttc gcattgcttt cataatctgc caatgtttgc tttgccattt cagtagcttg
     3181 ttctgcaagc attccatcaa aggatgaaac ggtttggaaa gaacgtgtgt caatgacatg
     3241 tgctaataga agttctccgt tatttcggat agctacattg accgcctttt taaaagctaa
     3301 ttcagcttca tcagaaccat ctacggcaac catgattttt ttgtattgtt gtaacataag
     3361 cattcactcc tcaagtcaat cgtattgtac tcttttattg tagaacactt tagggaaaaa
     3421 gagaattatt tactgctttg gttcagcaat tttttttgca aatcggatac tgaaggtggc
     3481 agaaacaata attacgatca gtaaaaagac caaggcaata aagcgttgat tgaaaaaagc
     3541 tgttaaaata cccattacgc ctaaaacagc atatgcagct ccgtaaaatt gtaggaatcc
     3601 ttgattttta tcatttttgt tgatcaatac aaaaaagata tcctgtttat taaataaata
     3661 atagctaacg aataataata ctaaagtggc gataatcatt aaaatcctaa tcatgaacaa
     3721 atcctccaaa ataagcaaca aatagaaaaa gcaatttgcc aagagcaaat tgctttattc
     3781 tttaaaagat ggtaatctcc gttaatgccg ttgtttgtcc tgtagtattg atcccgcgaa
     3841 ttcaagcagg tgggtcccat ggtcgtagct tcgaactacc aaatgataag gcatgttacc
     3901 agcagtgaca caagacgggt cccaagatat caataaaaat gttcggtcaa cacacaaaga
     3961 ggacaattcg aacatcacag atttcccacc tctatagtag catatgaatt taaaaagagc
     4021 aatgagagag ctcgcagaaa cagaaccgtt ttcttgtcta gttttttgga ggatgctttt
     4081 tccactcttt tatacggtaa tactgttgac ccagagcctg ttcatatttc cctgtatctt
     4141 ttggctgata atactgagca ttcttcagtt tatctggtaa atattgctga ttgacccaag
     4201 cctgatcaaa atgatgagga tactgataac ccacgccacg gttgagtgat ttcgcacctt
     4261 tgtaatggct atctctcaaa tgatcaggaa catctccagc tttgccctct cgaatatcag
     4321 caagtgctgc atctaatgcc atgtaggcag aattggattt tggcgacaag caaagatcga
     4381 cgacgacgtc tgctagtggg atacgtgcct caggcaatcc caatttttca gcagctaata
     4441 cagcgttcac tgtacgagcc gcagcagcag gattacctaa acctatatct tcataaccga
     4501 taaccatcag acgccggcaa atacttgcca gatcacctgc ctcaactaaa cgggcgagat
     4561 aatgaagtgc agcatccaca tcacttccac gaatcgattt ttgaaaagcc gaaataacgt
     4621 cataatgagc atctccattt ttgtcatggg taagagcttt tctttggaca cattcttcaa
     4681 tgatcgacag tgtaagatga atccttcctt ctttatctga aggagtcgat agtgtagcca
     4741 gttccagccc attcaatgca ctccgcaaat cgccgttcgt tgcacgcgaa aggtgaagaa
     4801 gagcatcttc gtctaactgg attgcttgtt gtccaaggcc gcgttcttta tcccttaaag
     4861 catgttcgac tgccaactgg atgtcttgtt ctgttaatgg tttgacttca aagatctgtg
     4921 tacggctgcg gatcgctgga ttgatggtaa tataaggatt ctcagtagtc gctccgatca
     4981 agatgattcg gccactttct aaatgaggaa gcagaaaatc ctgttttgtt ttatctaatc
     5041 ggtgtacttc atccaataag aggatcactg taccactcat ttttgcttct tccgctacaa
     5101 cttgtagatc cttttttgaa tctgtagcgg cgtttagcat gcgaaatgca taattagtag
     5161 agccggcgat cgcacttgcg atactcgttt ttccagtacc aggaggtcca tatagaatca
     5221 tggaagagag catgcgagca tcgaccatgc ggcgtatgat ttttccttcc ccgactagat
     5281 gctgctgacc aacgacttca tcgatcgttc gaggtctcat gcgataagcc aatggctgtt
     5341 gcataggatt ttcctccttc ttgtttttac gtcccttaat tatatcatat ttagcaagat
     5401 gcctagaaag aacagccttc tgttgttttg ttttcatagc ttatccaact aatattgtgt
     5461 atgataaagt aggtgagaaa aatgacggat aaacaaaaga tagatgaatt tttttggaat
     5521 aaaacgacgc cagaagtggc acgggattta ttggggatgt atttggagca tgacacacca
     5581 gaaggaagat tagctggata tattgtagac gcggaagcat acctaggtcc agaagacgag
     5641 gccgcccata gctatggttt aagaagaaca ccgcgtgtgc gggcaatgta tgaaaaacca
     5701 ggtacgatct acctttacac gatgcatact catcggattt tgaacatcat tactcagcca
     5761 gaagggattc ctcaaggagt aatgatacgt gcaatcgaac ccgccgcaat gattgatcaa
     5821 atgagtaaaa atcgtggcgg aaaaactggt ccagatatca gcaacggtcc gggaaaatta
     5881 gtggaagcat tagcaattcc tcaagaactt tatggtcagt ctatagcaga tagttcgtta
     5941 cgcttagttt ttgagaaaaa aaagacacca aaaaagatca atgctttgcc tagaatcggt
     6001 atcccaaaca aaggtgtatg gacagaaaag ccgttgcgtt ttgttgtgtc gggaaatcct
     6061 tatatctctt tacaaagaaa aaaccaaatc gagaaagatt ggggttggag gaaagaaaat
     6121 gaaaaagaag gatctattaa catatttaga tgaaataatt gaaaaacaag tgacagacta
     6181 tgatgtagct ttagattggg atacaaaaaa tcatacgatc gagatcgttg ttcgcttgtt
     6241 tgcagaaaat aaagcacaat ttgagattga tgatgcagaa gggatcgttt cgcaagaacc
     6301 aattatcgaa tttgaagacg gcgtacttct ttttaatcct cagaaatctg tttttgatga
     6361 acgcaattat ctagcagtaa tcccttatga aggaaaaaaa gggttggcaa aatcagtagc
     6421 tgattcttta gtggaatatc taaatgaagt attagctcaa gggcaaagcg atttgcttga
     6481 ttttttggat gaagacgatg acgaagctgt attcgagctt cactgggata atcagcaact
     6541 agcagaatta gtagaagtaa aacagcaaaa tgaagcagat acttatttac cgtacccaag
     6601 ttattaagga gtgacagata tgaaatggaa tgaagtaaaa gtagaaactg aaagtgaagc
     6661 agtcgaagca gttgctaata ttttgatgga ggcaggggcg agtggtgtcg cgattgaaga
     6721 ttctttagat gttgaacatt ttcagtcgga tcaatatggt gagattttag ataaaaatga
     6781 ttttacatcg ctgaaagaag gcgcactagt catggcttat tttccggaaa cgactttctt
     6841 accggaaatc ttgcctttta tcaaagatcg tatcacacaa ttacctgagt ttggtctagc
     6901 tattggaaaa aataaagtaa cagttagtga agtagaggaa agcaactggg caacagcctg
     6961 gaagaaatac tatcatccgg tgcgtattac gcgaatgctg acggttgcgc caagctggga
     7021 aaactaccag acagaagatc ctctagaaaa aattatcaca ctagatcctg gtatggcatt
     7081 tggcacaggc actcatccca cgacttcctt gacattgaca gcactagaaa cagtcttgcg
     7141 tggtggtgaa acattgctag atgtgggaac tgggtcgggc gtgctaagta ttgcagcaaa
     7201 atatcttggt gcaaaagatg tctatgcctt tgatttagac gaagtagccg ttcgttcggc
     7261 gaaagaaaat atggatatga atgaaatagc caaagatgtc catgtatcag ctaacgatct
     7321 tttgacagga atcgagattg aaagtgatgt aatcgttgcg aacattttag cagatatcat
     7381 cttattgatg atcccagatg cttggcgttt actgaagcaa acaggcacgc tgatcgtttc
     7441 aggaatcatt gaagagaaga agcaaatggt tctggatgcc atgttagaac aagggtttat
     7501 cgtggatcaa attttccagc aaaaagactg gtatgcaatt attttgaaaa aaccagaggt
     7561 ggaatagatg cagcggtatt ttttaaaaga atcatatgaa tcaaaagact attttgtttt
     7621 agaaggggaa aactatcacc acattgtacg ggttatgagg atggaacccg aggatgaagt
     7681 ttttttggca ttcaatgatc aaatagccat tgttgcagaa atcacggaga ttactgaaga
     7741 ggctgtttat ctgaaagaaa ttaaaaaaga agaaaatcaa aaggaactgc caagttcgat
     7801 taccatagcg agtggctatc ctaaagggga aaagctggat tggatcgttc agaaatccac
     7861 tgaattagga gcgcaagctt ttcttggttt cccggcatca gcctctgtcg ttaaatggga
     7921 tgcaaaaaaa cgtatgaaac gacaacaacg gcttgaaaag attgctcaag aggcagcgga
     7981 acaatcccat cggcagatcg tgccatcagt agagttattg gaaacgaaac aagatttatt
     8041 tgagcggcta gatgattatg acgtggtctt ggttgcttat gaggaatcag caaagcaagg
     8101 cgaaaaatca aatgcagcca aaattcttag tgaaacaaaa ccagatagtc gaatcttagc
     8161 tgtctttgga ccagaaggtg gattatcccg cgatgaagtc gattattttg tagaacacaa
     8221 agggatactt tgtggattag gaccaagaat ccttcgtaca gaaacagcac ctctttattt
     8281 attaagtgct gctagctatc aatgggaatt aatgaatcct tctttcagct aacgttcttc
     8341 attaataaaa gagtatcgac agaaagaatt gaaaaaatag tcaacaattt gtataataga
     8401 aacaattatt tatacaatgg atacgaaaaa tcgtttgaaa aagcttttaa aaagcgaaca
     8461 tttccgtaaa aaaacgtata actgcacgga ttttgggaca attatcaaac agatatttgt
     8521 cccgattccc gttttaccga aaaaagaaag ggggatcgat tcctgtgaca aaagaagaaa
     8581 ttttaacggg acccggtgtc ataaagctcg ttagtcaata catgggtccc gaacatgtag
     8641 catttgttga gaaagcatgc gaatacgcga ctgctgcaca tgacggacag tttagaaaat
     8701 caggcgaacc ctacattatt catcctatcc aagtcgcagg tatattagca gatttaaaaa
     8761 tggatcccca tacagtggct acaggcttct tacatgatgt tgttgaagat acagaaatca
     8821 ctttagaaga tctgagagaa gaatttggcg atgacgttgc tatgttagtg gacggcgtaa
     8881 ccaaattagg gaaaataaaa tataaatccc acgaagagca gctagcagaa aaccaccgaa
     8941 agatgctgct agcaatggct caagatttac gagttatcat ggtcaaatta gctgatagac
     9001 tacataacat gcgtacgttg aagcacctgc gagaagataa gcaaagaagg atcgctcagg
     9061 aaactttaga aatctatgcg cctcttgcac atcgtctagg gatcagccgg atcaaatggg
     9121 aattagaaga tacagcactt cgttatctaa atccaaaaca gtattaccgt atcgtccact
     9181 tgatgcagac gaagagagaa gaacgtgaaa aatacgtaag tggtactgtt gaagatattc
     9241 gaatagcgac agaagagctg gggatttttg cagaaatcta tggacggcca aaacacattt
     9301 attcaatcta tcgtaaaatg aaagatcaga aaaagcagtt caacgaaatt tatgacttgc
     9361 tagctattcg agtgatcgta gattcaatca aggattgtta tgctgtatta ggagcaatcc
     9421 acacaaaatg gaaaccaatg cccggcagat ttaaggatta tattgccatg ccaaaagcta
     9481 atatgtacca atctttgcat actaccgtga tcggtccggc aggaaatccg gtagaaattc
     9541 aaatccgaac acaggaaatg catgaaatcg ctgaattcgg ggttgctgca cactgggcct
     9601 ataaagaagg aaaaaatgaa aaagtagaac cagatggtat gacgaaacaa ttaagctggt
     9661 tccatgagat actcgaactt caagacgaaa gctatgatgc ttctgaattt atggaaggcg
     9721 taaaaggaga tatctttagt gataaagtct acgtcttcac accaaaagga gacgttactg
     9781 agttaccaaa aggatccgga ccattggact ttgcatacag tatccataca gatatcggta
     9841 acaaaaccac tggtgcaaaa gtaaatggca aaatggtgca gcttgattac aaattgaaaa
     9901 acggagatat cattgagatc atgacttctc caaattcatt tggcccaagt cgcgactggt
     9961 tgaaattagt tgctactagc aaagcaagaa ataagatcaa acgtttcttc aaagcccaag
    10021 atcgagaaga aaatgtgatc aaaggccacg aatccgtggt caaatgtatt acagatctag
    10081 gatttacgcc taaagatatt ttgacgaaga acaaactgca agaagcactc gatcgtttta
    10141 attatcaaac agaagatgat ctctatgcag ctgtagggta tggagaagtt agccccttga
    10201 cgatggccaa tcgtctgact gaaaaagaac gtaaagaaca aaaaatcgag cagcaaaagc
    10261 aagaagcaga agaaatcatg aatcagccga aaaaagaacc tgacaaaatg aaagtacgtc
    10321 atgaaggtgg cgttgtcatt caaggggtag aaaacttact aattcgtatc agtcgctgct
    10381 gtaatccgat tcctggtgat gatatcgttg gttatatcac taaaggcaga gggatatcca
    10441 ttcatcgtcg agattgtccg aatgttcagc ctgacaaacc aaatgtagca gaacgtttga
    10501 ttgaagtcga atgggaagat acatcgaata cacgaaaaga gtatgatgca gatttggaaa
    10561 tttacggcta taatcgttca ggcttattga atgatgtact tcaaacagtc aatgcgctaa
    10621 cgaaaaatct caacagcgtt gaagcacgga cgaataaaga taaaatggcg acgatccatt
    10681 tgacggttgg tatccagaat ttatcccacc taaagagtat cgtggataaa atcaaagcag
    10741 tacctgatgt ctacagtgta cgccggacga atggatagga ggacagatat gagagcagtt
    10801 gttcaacggg taagtaaagc tgctgtgtca gtcgatcagc aagaaattgg aaaaattggc
    10861 tacggcctac ttgttttatt aggcattcat gaaaccgata cgcaaaaaga tgtggactat
    10921 ctggtaaaaa aaattgcaca aatgcgtatt tttgaagatg aacaaggaaa aatgaatatt
    10981 agtgtagaag atgtgggagg acaaatttta agtatctctc aattcacact gtttgcggat
    11041 acgaaaaaag gaaatcgacc aagctttgtg gaagccgcac gaccagaaac agctattcct
    11101 ttatacgaat cattcaatga aggaatcaga agtcgcggca tcacagtcgc taccggacaa
    11161 ttcggagcag atatgtcggt ttctctcgtc aatgatggac ctgtaacgat cattattgat
    11221 tctaaaaaca aataaagtaa tctggttttc actagtaaaa actatgaaac acgtcgctta
    11281 agttagactg ataaactcta acttaagtgg cttttttgtc tgcaaaaagc gcttcttttg
    11341 tcagcagtcg gaaaaaaagt taagcttaaa ttatacaagt tattttttat aggaggttgc
    11401 tgaagatgaa agaagtacca gtagtagata tcaaaatcac agatttaaaa aaattgtatc
    11461 aaaaaggata caacatgttg gaggagttac gccatgaagc ggacgctccc gtcgtcaaag
    11521 caaagatttt caataaagaa gctatcacga tctacggatc atcagcagca aaagtatttt
    11581 atgatccaag gaatttcaaa cgaaaaggtg ctatgccaaa attagtattg aagacacttt
    11641 ttggtcaagg tggagtacag actttggatg gagctgctca tcaccatcgg aaaaatattt
    11701 ttatggatct aatgacaccg gaaagaatgg aagattatca tcgtatctta gataagaatc
    11761 tgacacaagc acttgaagca cagcatggtc aatttgaact atttgattta agtaagatgg
    11821 ttttctttac atctatttgc gagtgggcag gtatcaattt atcagcgatt tctaaagatg
    11881 aggtcgaaaa attagcagaa tatcaaatct ctatgatcag cggaacgttt acttctccga
    11941 ttgatcacat aaaaggtgtc gaaaatcgca aaaaatcaga aaaatgggcg caaggcttga
    12001 ttgaagaagc tcgacaaaat cctgtggctg gaaaagaaaa tgtggcatta tatgcttttg
    12061 ccaatgccac agatttagat ggtcagcttt tacctctgga agtggcagct gttgaattat
    12121 tgaacatcat ccgtccaacg gttgctctaa ctgtgtgggc ggcattgatg ggacatgcac
    12181 ttttcagtag accagatttg tatcagcaat taaaaaatga cttttcgacg ttacaagatc
    12241 cgtttattca agaaatgaga cgctattatc ctttcttccc aatgctacct gcgatttcgc
    12301 tgaaagaagt agaagtagat ggttatcgta taccagaagg cagttgggtt attttagatt
    12361 tatatggaac agaccatgat gaacgaacag ttgaagcgcc agattccttt atgatcaaac
    12421 ggtatgtagg gaaagcaaaa gatatttcct ataaagaaga atatgagatg attgcacaag
    12481 gcgggggaaa ttttcgacaa atgcatcgct gtgcaggaga atggatcact cttcatagtc
    12541 tacgtgtgtt ttccgatcag ctagtaaata agtttgagtt ctctgtacct gaacaagatt
    12601 ggacgattcc attcaatcag tttccgactt atccgaatag ccgagcgcta ctttacaaga
    12661 attagattag cataaactta tcgaaaaaaa ggaagatcaa acagtgctga tcttcctttt
    12721 ttcatattac tataaattga taaggtcaat tgatcaaaat ccacgaaatg cttcatcaat
    12781 ggtttgataa tcttcattgg ataaatgcaa atctaaagct tggacattgt cagatacttg
    12841 ctctggacgc ttagctccag gtataactac agacactcgt ggattcttca tgtaccaagc
    12901 taaaacaagt tgcgcaggag tggcttggta tcttttagca atggggcgaa gtacatcgac
    12961 agcggttaaa atttctctga aacgttctcc ttggaaatct ggatttcttt ttcgaggatc
    13021 tccttcacca aattgttttt cctcaccaag ttcatatttt cctgtcagca aaccggaagc
    13081 aagaggaaaa tagggaacaa aactgatttt gtttttttca agataaggga ataattcttt
    13141 ttcagcctgc cgatggatca aactgtattt atcttctact acatcgacat atccatctgc
    13201 attcgcttct ttcaattgtt ctaacgtgaa gtttgacaca ccgactgctc ggatttttcc
    13261 tgcttctttt aattcgtgca atgtagcaac tgattcattt tttggtgtgc tttcatccgg
    13321 aaagtggata taaaaaatgt cgatgtaatc agtttgcaga cgttttagtg catcttccac
    13381 cgattgtttg agaaactcag gagaattatc aaatgttcgc cctttatttg gtacatgagc
    13441 agcttttgta gcgatgacga cacgtgagcg atcatattct ttttccttca gtacctcgcc
    13501 aatcagttct tcggaacggc cgtttccgta agcataagcg gtatcgatca aattgatccc
    13561 gctattcaaa gctgtacgaa ctacttgttt tcctgtctca tcttccaaac cgtcaaaaag
    13621 attatgtcct ccaacggcat ttgctcctaa tccaagctgt tctgcgtaga cttgtgaatg
    13681 tccgattctt atttctgcca tagctgattc ctcctttttg tctagtctaa aacaaaatac
    13741 caaaaagctg aactaaaacg tttttgtgag gggaaatagt cagatatctc ttgactttga
    13801 gactggtttt tagtaggtta taagtaatat cgaatgtcaa tgaaagagcg agtaagtttt
    13861 tgaaacttta caagagagga tcatcgaggc tgaaaatgat ctaaagtttc attagcctaa
    13921 agacactctg gagatggacg agtgaaaagc cgtcccgcgt acgagcgtta ttcgtaagag
    13981 gaaagagaaa tctttcaaaa ttaggtggta ccgtgtatga atttcattca cccttgttca
    14041 tgatgaacaa gggtgatttt tttatttaca tagctaagaa agggagagaa aactatgagt
    14101 ttccaaagac caaaaggaac aaatgatatt ttgccaggcg aatcagaaaa atggcagttt
    14161 gtcgaagaaa cagcacgttt attattcaga gattatcagt ataatgaaat gcgtacaccg
    14221 atttttgaac actatgaagt gatatcccga agtgtagggg atacaacgga tatcgtttca
    14281 aaagagatgt atgattttta tgacaaaggg gaacgccatg tgacgcttcg accagaaggt
    14341 acagcaccaa tcgttcgttc ttttgttgag cataaattgt tcggcccaga gtatgcgaaa
    14401 ccgttcaaaa ctttttatat gggaccaatg tttcgctacg aacgccctca aaaaggacgg
    14461 ttacgtcaat tccatcaaat cggagtagaa gcatttggat cagaaaaccc tgcaactgat
    14521 gtggagagca tggtgatggc acttgatttc ttcaaacagt tggggatcac gcaaatccgg
    14581 ctagtcatca attcattagg tgataaagaa acgcgtcaag cctatcgtca agcattgatc
    14641 gactatcttt taccttttga atcagagctg agtgaagact ctaaacgccg ccttcatgaa
    14701 aatccattac gcgtattgga cagtaaagac aaacgagatc aaaaatttgt ggcagaagct
    14761 ccatctatct tagattatct aagcgagcct gctcaaaaac attttgatac cgttgtttcc
    14821 atgctggatg cattggctgt tccttatgaa atcgacagta atatggttcg cggactagat
    14881 tattacacac atacgatttt tgaaatcatg agtgacgcac caaaaatggg ggcacaagca
    14941 acgatttgcg caggcggacg ttatgacaat ctagtggaag aactaggtgg tccttcaaca
    15001 cctggcttcg gatttgcaat gggtatcgag cgattactct tgactatgga ggcagaggaa
    15061 gtagttatcc ctgcatttaa tgagttggat gcttacgtgg tcgcactcgg agacgaaaca
    15121 aatatcgaag cattgaaagt ggtccaagcg attcgaaact ttggtttttc agctgaccgc
    15181 gacttcatga accgtaaagc aaaagcgcaa tttaagacag cagataaagc gaatgccaaa
    15241 ttagtattgg tgatcggtga agatgagctt gctagtcaaa cgattaatgt caaagcaatg
    15301 gcaagccgaa aagaaaaaag ttttccatta gaagagatat atgaacattt tgatgaagtc
    15361 tatgatgaaa tgactttatt cgagtaggag gaaaaaaata aatggcacaa agaacgatct
    15421 attgcggatt agtttccgaa caacaattag aacaaacagt tacattaaaa ggatgggtac
    15481 aaaaaagacg agaccttggg ggagtaatct ttatcgacct acgtgatcgt gaaggaatcg
    15541 ttcaaatcgt ctttaatcct gaaatcaaca aagaagcatg ggaaatcgcg gataaatgtc
    15601 gcagtgaata cgtgatcgaa gtgactgggg aagttagaag acgcggagaa aatgcagtaa
    15661 atcctaaaat ggccactggt gaattcgaag tgattgcaag tgaaatcgtg attttgaatc
    15721 aagcaaaaac cccgccattt caaattgaag atgatcaagc aatcagtgac gaaatacgtt
    15781 tgaaatatcg ttatttggat ctgcgtcgtc caaaaatgac taaaaacatc aaattaagaa
    15841 atgcgacaac aaaagcgatt cgtcattatt tagatgataa tgactttctc gatatcgaaa
    15901 caccttacct agggaaatct acaccagaag gcgcaagaga ctacctagtt ccttcacgtg
    15961 tccatgctgg acatttctat gcgttgccgc aatctcctca attgtttaaa caattgctga
    16021 tgaatgctgg attcgaccgt tattatcaaa tcgttcgttg tttccgcgat gaagatttac
    16081 gtggagaccg tcagcctgaa tttacgcaag tagatattga aacaacgttc ttaacagcag
    16141 aagaaatcca aacttacaca gagggattga ttgcgaaggt aatgaaagaa gttcgcggta
    16201 tcgaagtcac attgccattt cctcgtatga cctatgatga agcaatggct cgctacggca
    16261 gcgacaaacc ggatacacgt tttgatatgg aattgatcga tctaagcgat acagtcaaag
    16321 aagtagaatt caaagtcttc caaatggctc ttgaaaacgg cggtgtcgtg aaagcattga
    16381 atgctaaagg cgctgcagat cgctactcac gaaaagacat ggatcagtta ggtcaatatg
    16441 taggacaatt cggagctaag gggcttgcat ggctgaaagt agaagaagac ggattgaaag
    16501 gtccaattgc taaattcatg ggagaagcaa cagaagcaat cattaaagca acagatgcaa
    16561 aaccaggtga cttgctgatg tttggcgcag ataaaagtga aatcgtcgct gctgcattag
    16621 gtgctatccg gacacgttta ggaaaagaat taggactaat tgatgaatcg aaattcaatt
    16681 tcttatgggt aactgattgg ccacaatttg aattttcaga agaagaaggt cgatatgtct
    16741 ctgctcacca tccattcacg atgccaaaag aagaagatat cccgttattg gctgaagacc
    16801 ctgcaaaagt ctatgcagaa gcttatgata tcgtcttgaa cggttatgaa ctaggcggag
    16861 gttctatccg tattcatact cgtgagcttc aagaaaaagt actagaaact ctaggcttta
    16921 caaaagaaca aatggaagaa caatttggtt tcttgttaac agctttagat tacggattcc
    16981 ctccacatgg cggtattgca ttaggattgg accgttttgc aatgttatta gccggagaag
    17041 ataatattcg tgaagtcatt gcctttccta aaaacggaaa agcagcagat gtgatgagtg
    17101 atgcaccaag tatggtttcg cctttacaat tgttcgaatt gaatattgat gtaacgaata
    17161 tagaagaata gccgataaaa aggtaaaaag gatttgtcga atacgcttct tttgacaacc
    17221 ttgatttaat tgaaaaacac tttataatga agccataaca tataaagcgg ggcttttcca
    17281 tgaaaaaaac attcgaaaat tgggcttatc atgattacac gaccaagctt tcggtatcta
    17341 tcgtttatgc gttgatcgca tcagtcgcat tgaatttctt ttatcagcca ggacatattt
    17401 attcaagtgg tattactggt ctagctcaga ttttgacgac tttgtcaaaa tcactgatag
    17461 gattcgaagt accggtatca acaactttgt atttactgaa tataccttta tttgtattgg
    17521 cgtggttaaa aatcgggaag aagtttacaa ttttcacttt tatcacagtc actttgacct
    17581 ccctttgtat ccatatttta ccggaaacag tcctatctac cgatccaatc atctgtgcgt
    17641 tgtttggggg ggctgtgatg ggactaggta ttgggctagc gttgagaaac ggcctatcat
    17701 cgggcggtct agatatcgtg agtatcacga ttcgtaaaaa aacaggaaag tcgattggtt
    17761 ctatttcgat ctacttcaat gcattgatcg tttttgttgc cggaattcta tttggctggc
    17821 aatatatgtt ttacagtacg ttgtctattt tcgtcagtgg aaaagtaact gatgtcgttt
    17881 atactaaaca aaagaagatg caggtaatga tcgtaacgaa acatccagaa aaagtagtaa
    17941 atgaaatcca aaactgtatg cgtagaggga tcacgatcat caatgaagca gaaggtgctt
    18001 ataagcatga taagcagaca gtattgatga cgatcgttac acgatacgaa ctgcctctac
    18061 ttagagaagc aatgaaagct tctgatccac aagcttttgt aagtattgca gaaaacgtcc
    18121 agatacttgg tcgtttctat gaggatggca tttagatcga gtttgacaaa aattcatgaa
    18181 atactgctgt agacagacgt tcgtttgtct gcagtttttt tctaccatag tagaatcagc
    18241 aaagaaaaac tgctttttta tttgaaattt tcttaaagtt gaaaaaaagc atgtttattt
    18301 ttttaaattt atgggatgat agaaaaaaat agtagagaaa ggacacgatt ctacttgcta
    18361 ttttcactta atacccgtat attacgaatg aaaaaagttt atgaaaagtt ggaatattga
    18421 atggaaaaac aaaaaaggtt aaaaaacagc cgctacatca ctgggtttga tgggctccgt
    18481 tcattagctg tggtgggtgt tatcttctat caccttctgc caactagtat gaaaggcggc
    18541 tatttgggcg ttccgatttt tttcgtcgta tctggttatt tgatcacgga tctgctgcgg
    18601 caggaatggg aacaaaatgg aaaaatcaat atttggcaat tttatgtcag aagaatgaag
    18661 agactctatc cggcacttgt ttttatgctt gtgacagctt cggcttatat cactttgttt
    18721 cagcgagggc tgttaaacaa tcttcgagga gttgtggtca gcagcttact gtatgtcaac
    18781 aactggtggc aaatcaataa cggtttatct tatttcgatc gatttgctaa tgagtcaccg
    18841 tttacgcata tctggtcttt agcagtagaa ggtcaaaact atttggtttg gccgatcttg
    18901 tttgtattgt tgatggtatt tgttcgtaaa agaaaatgga tcttttatac tgtgttaggt
    18961 acatccttcc tctccgccat cttgatgatg atcttgtata caccaggagg cgatccaacc
    19021 cgtgtttatt acggtaccga cactcgtctg ttttctatat ggttgggaag tgcgcttgcg
    19081 tttgtttggc caagtacgcg tttaaagaaa aacatcccga aacaagcgaa aaatattctg
    19141 aatatcgctg gaggaatctc gcttttggca ctgattcttt gcttcttctt tttagatgat
    19201 cattacaatt tcatctatta cggaggaatg ctcctagtga gtctgttatg cgtagtgcta
    19261 gttgcagtga cagcacaccc cggagcaagt ttgaatcgat ggctgacaaa tccagtattt
    19321 acttggattg gcaaacgaag ctatggcatt tatctgtatc agtttccagt tatgattttt
    19381 tatgaagcaa aggtgactga tattgccgat catgttttgt tgcatacttt agtggaaatc
    19441 acattgattc taatcatcag tgaactttct tatcgatatg tggagcgtcc gctagcacgt
    19501 tttgattatg gtcgtacatt ccaagtgatg aaaggatggt tcagcaaacc gatcatcagt
    19561 aagagcaaac catgggttgt tccaggcaca ttacttgtag caattgcact cgttggtttc
    19621 gttacggcac cgaaaaacgc tgtaacagcc gatcaaaaac aactgcaggc aaaaatcgct
    19681 gaaagtaaaa aattagcaga agaaaccaga aacaattcag aaaacaccac tagttcggtt
    19741 gaccaagcga tccttgataa gtacgagttg tccaaggcac aagggaataa ggcacaaaaa
    19801 ttagaactaa cggcatttgg tgattcagtt atgttggatg ctgcaacgga tctacaagaa
    19861 ctttatccaa aagtagtggt agatggagat gtcggacgac aattatatgc cagtgtaccg
    19921 tacattgaaa aattaaagga acaagatttg ttgaaagaca ctgttctgat cggactagga
    19981 acgaatggct cattctcgga agctcagttt gatagtgtga tggatgcaat cggtgatcga
    20041 aaagtatact gggtcaatgt ccgagtaccg acgaagcgct ggcaaaatga cgtgaatgcg
    20101 atgcttgaac agatgacgaa aaaatacgac aatatgacgc tgattgattg gtacagctac
    20161 agtaatgacc atagtgactg gttttatgat gatcaagtcc atccaaatcc tgaagggatg
    20221 acacattaca ttcacttagt cagcgaagcc ttgctaggag gaagttcaag tacgaacagc
    20281 agtgagacaa acgtgacaga atgatagcta caaaaaactt caggcaaaaa catttgtttt
    20341 tgcctgaagt ttttttatat ttaccgtgtt tttaacattc atatagattc aggaagcaaa
    20401 acggttgagt gtgtctgtaa ataacgttct ttttgttttc gggtgagttt tttgaatgca
    20461 gcttctgctt ttgttgcttc gcttcttgaa gaaaatactt ctgcatggat catcttaaca
    20521 ggtcttcttt tttgaagacg agtatattta gcaccagtac cattgttatg ttcctctaat
    20581 cgtctagaaa gatcggttgt atatccacca taaaatgtct gatctttaca gaataatacg
    20641 taaaaataat gttcatttgc catagagcat ctcacgaatc tctgcagtgt attctccatc
    20701 catattgtaa gtgatcaatg gtggagcgac tttgaatccg tctaatttgc cttctttgat
    20761 tccttcaact aacaaaatat ttgcctcttt tccaggtttt gggtaaacaa attggatcct
    20821 tttaggtgca attcgatagt cttccatgac ctgaatgatt tctaagaacc gatcaggacg
    20881 atggaccata gtaaagcgtc catttgtttt tagtagctta cttgaaatat cgatcacttc
    20941 ttctaacgtc gtatgaatct catgacgggc gattgccagg tgtttattag ggttcgtttt
    21001 atttgttggc agccctttga aataaggtgg attacagacg actaagtcac aagaattatg
    21061 tcgaacgaca gataagctgt ctttcagatc gatggtgtgg acagtcattt gttcatttaa
    21121 atggttaagc gcgatactac gtcttgccat gtctgctaga cgaggttgaa gttcgatagc
    21181 gtcgatagcc gcctgggttc gtttacttaa aaaaagtcca actgcgccat ttcctgcaca
    21241 aaggtcaaca atcttccctt ttttaggtac acgggaaaag tgagcaagca gcacagcatc
    21301 taaagaaaaa gaaaacactt tggaactttg gataatcttg atatcttctg cgtataattg
    21361 gtcgattcgt tcgtcttcgt gaagcatgga ttccctccaa aatgaaaaaa atagtcgtct
    21421 gtattatact atttttatta aaaagaacca aggtggcttg tcaaacctct attttttatg
    21481 catactatga ccataagaag ggaaaggaag agactatgtt ttatcgattt atgcgcgggg
    21541 tagttcgagt actattagcc ttagttaatg gaaatgcccg ctatgagaat aaagaagttt
    21601 taccgcaaga tgagaattac atactagttg ctccccatcg tacttggtgg gacccattat
    21661 atctggcggt agctgcagca cccaaggaat tcagctttat ggcaaaagaa gaactgttca
    21721 aaaatccagt tctgcgattt atattaactc gatccaatgc ttttccagtt aaaagagaaa
    21781 atccaggacc aagcgtcatt aaaacacctg taaagatttt gaaatcgaca aatttgagtt
    21841 tgatcatgtt tcccagtggg acaagacatg ctacagaact taaaggcgga atggcattga
    21901 tttccagaat gtctaaagcg aaaatcgtgc cagctgtata tcaaggtccg ttgacacttg
    21961 gggatctgtt caagcgtcag agagtgacag tgcgtttcgg cgaaccaatc gacttatcag
    22021 atatcaagaa aacggataaa gaaggtatgg cggagatcga acgacgaaca caagcagctt
    22081 ttgacaaatt ggacaaagaa gtgaaccctg atttcaaata tgaagtcaaa aaaaaataaa
    22141 atgataagtt tatgcgtggg cggtcttttg aatctactgt cctgttcagt tcagactttt
    22201 tcaatgaaaa cgagggagag acgtcaagtc tgccctcttt ttgcgttcca atatgtaaaa
    22261 tagaagacag gtgtcatgta atggatcaaa gtgttataat aattagaagg acattttttg
    22321 aggtgattga atgcgttttt tacatacagc cgattggcat atcggaaaaa aactacaagg
    22381 ttatgacttg attgaagacc agcgtcatgt actatcttct atactagata tcgcgaaaga
    22441 agaaaaagta gaggcaatcg tgattgccgg agacttatat gataggagtg tcccagctgt
    22501 tgatgccgta gaattataca atgaattgat ggtcgattgg aatttaaagg aaaagatccc
    22561 gatttttgct atatcaggaa atcacgatag cagtacgaga ctttcagcgg gtacgccatg
    22621 gttcagttat tcgaactatt ttttgcgaac acagctagca gaagcatttg aaccagtcga
    22681 gtttaaaaac gtacaatttt tctttcttcc ttactttgaa ccaattgcag cgagattata
    22741 ttttgaagat gacaatatcc gaacgatcaa gcaagcggta gccaaggtga tagataaaat
    22801 gcaggagcta tttgattcag aaaaaaaaca agtattgatt agccattttt ttgtaatggg
    22861 aagtgtaaaa tctgattcgg aaacgaaaat cgaagtaggc gggttggatt cgatccctgg
    22921 tgaactactt acaccgtttg attatgtagc actaggacat ttgcataatc aagctgcttt
    22981 gagacaggat aatgctcgct atagcggttc cccattgaaa ttctctttat ctgagatcaa
    23041 ccaaaaaaaa ggcgtatgga tcgttgacct gacaacaggc ctggagttga attttaggga
    23101 gatcgaaccc ctgcatgata ttgaagaaat aacagcgagt ttcaaagaat tgctggatcc
    23161 tgccttttat cagtccgtca atcgggagaa ttacatctat atctatctca ctgatagaac
    23221 gatcatcccg aatatgatga accagcttcg gcaaatatat cctcgtattt tggggattga
    23281 acgggcaaac ggtcgagcag aacaaacaaa gcgcaaacga aaagtactag aaatgaaaga
    23341 tcctcaaaaa ttagcagaag cattttttag tgaaatgacg gaagagcagc taactgcaaa
    23401 ccaagaaaaa tggctcggag aaacgttgca agaaatcatg gaaatggaat gatcaagaat
    23461 gaaaccgaaa aaattacaac ttaaaaattt tggtcctttt atcaatgaga ccgtagattt
    23521 ttcacgtttg acagaagcac cgcttttttt gatcagcggg aagacaggag ctggcaaaac
    23581 gacgattttt gatgggatca ccttcgcact ttttggtgaa acttccggaa gacttcgctc
    23641 tggaaaagag atgcgttctt tgtttgctac accagacgaa gaaacaagcg tgactttttc
    23701 ttttgaacat cagcagatga gctatgaaat cgaacgaaaa ccagaacaag ttttagcaaa
    23761 acgaaaagga aacggaacaa aaaaacaggc agccaaagta acgttgacca tttttgatgg
    23821 gaaaggaaaa gaaataaaac aaatcacaaa acgtacagaa gcagatcagt tgatcagaga
    23881 actgatgaat ctcgatgcga aacagttttc tcaaatcgtt ttactacccc aaggagaatt
    23941 ccgtaatttt ttggtttcat caagcagtga taaagagact gttttacgtc atttattcgg
    24001 tactcaattt ttccaacagt tcaatgagcg attaaaagaa aaagcaaaac aacagcaaca
    24061 aaaattggat catttagagc aagagctgca tcttctgcaa aaaaggtttg ttttagtcaa
    24121 agaagaggaa ccagcagcag ggtttgaagc cgttttagcc caatggacaa accatcaggc
    24181 aatactcgaa aaacaaatca cggaagaaaa agaagctcta acggaacttc aggaaaaaca
    24241 aaagcaacta gaaaattcct actatcgttt tgaaagcttg aaaaaaagtt atgaagaaaa
    24301 aacgatatta ataaacaagc aaaaggaatt agctgctcaa aaagacgaaa tagaggaaaa
    24361 gaaacgatgg attgggtatt atgaatttac gcaacgatta gtcgaaccaa tccagcgcct
    24421 acaagaaata aaagttgaaa aaatagaact gtcacaaaaa agtagtgagc aaacaaagaa
    24481 actagcgatg actacgcaga attatgaaga gtggcaaaaa aatgaagcaa accgcgtacg
    24541 ccgacaaaca aaaatcaaag aagctgctca aaaactgcaa aacaaacaga tgatggtgcc
    24601 aattgtcgaa tccttacgta gtaaaaaaca agacgcagaa caattaacaa tttcattgga
    24661 taaagaagaa ttgaatgccg agaagctgta tcaaaatctc gctatctatc aggaaagaac
    24721 tcgaattctt caagaaaatt tactggatca ggaaaaactg caagaagatc gtcttgcttt
    24781 tggacagtta aaatatctag atgaaaagca gcaagaaata gaaaaacaaa aagcaaaaga
    24841 cactgggtcg ctcgcacaga taagagaaga actcaaaaaa gcaactgaag aaataacaaa
    24901 aaatagtcag ctattgaaag aacagacaga acaagcagaa cagctgaaaa gccaatgggc
    24961 aaagcagcaa attgctcgtc ttcagctttt tttgcgtcca ggggagccat gtcttgtttg
    25021 tggatcaacg gaacacccca tacaacaatt aaaacacgaa cagccaaata aggaagatat
    25081 cttaaaagta gaacaacaac tggcagaagc tgagtctgct gtagaaagaa ctcagcaaat
    25141 cattgccaaa agtgaagcac atgaaaataa tcttcaacaa caattatcag atttagaaca
    25201 aaaaatcatg gcaacgaata tacaattggc tgaacaagaa gaacacttgc aacaggcttt
    25261 gaaaaaggca tttcctgaga taacagaaca aaaaacaaaa acaactcttg tagtaatcga
    25321 acaatcttta gatagacgaa aaagcaagat ggccaatgca agaatagagc tggaagaaat
    25381 aagacaacaa gcagaaaagc tcgaacaaca attagctgtc tacaaacagc agcagacaga
    25441 aaaaaataat cagctccagc aactgcaagg agaaatcaaa agtttagaac aacaattgca
    25501 agatacggat agtaactgtt tagaagagga gatacgacga cttgaaaaac agttagaaga
    25561 agactcgaat caactagctg ttgagcagaa agcaggagtg caattgttgc aagatcttca
    25621 tacgcttcgg acacagcttg atcttcaaca tgaacaaata gaaaaaatag atgaaaaaat
    25681 agaaaaggaa caagaaagaa tagctaaaaa gttagaaagc cagtcgtatt tttcaacaat
    25741 tgaagaaatc tgtgcatttg cacaacagca agcacaatat aaagaaaacc aacgtacagt
    25801 tacttcttat catgaagagc aattagtgac agctgaccgg ctgcgtcaat tagaaaatat
    25861 ccagctaact gagacattcc cagatacaga agagatcagc caacagttag aagaaataag
    25921 acgtatgatc gaaataaagc aagaaaagat atacggttta caagaacaaa aaattagtaa
    25981 tcaaaaaatc tacgatgaat gtctttcgat ttatcacaca agccagacga aattagatga
    26041 gcttacgcaa ttacagcagc tttcgcaaac attcaacgga gaaaatccta agaagactag
    26101 tttggaacgg tatgtgcttc aggtttatct tcaagaagtg ttacaagtag caaatgatca
    26161 tctccaacgt ttaacgaaaa atcgttatca atttgagcta gcggatacca ttggcagtta
    26221 tcgagggaaa accggattag aaatcaatat ctatgatgat caggcaggta tgtctcgaag
    26281 tgcgcatacc ctgtctggcg gtgagagttt tattgcagca ctttctttag cattagctct
    26341 tgccgatgtg atccagacac aagctggtgg tgtagcaatc gatgcattgt ttattgatga
    26401 aggatttggg tcattagatg aagaagcttt ggaaatggca atggaagctt tagaaacgat
    26461 cgaaagtgaa ggaagaatga tcggcatcat tagtcatgta agagaattga aagaacgagt
    26521 attacaacaa ataagaattg atacagaagg tagcggacaa agcaaaatac gctatcaact
    26581 tggataaaaa gggagattgg taactatgaa atggagtatt ccagaaaaag tcattgcacg
    26641 tggacgtcaa tacttgcaag aaggccgagt gttatcggtt gtccctgacc aagaagaaaa
    26701 cgtgtggcat gcagaagtct tgggcagtga attgtatatg gttgatttgg atggtacagc
    26761 aaaagagata gattactgcc aatgtcccta ttgggaggaa cataaatatt gtaagcatac
    26821 agttgctgtg gaactttatt tacggtcgaa aaatctatct cgtatcatga aaaaagacca
    26881 gccaatgcta gaaccggtaa aagcaacaag tgagggagaa atcattgcaa aaggtttttc
    26941 tcgtctaaaa acacaaaaca atcctcaagc ggtgcaacct ttgattattg aataccaagt
    27001 agaaacgatt gagactaatc agtatcaccc agagctttct attttggcac tttacttgcg
    27061 tatcggttca ttggcatcac cgaaaaaaac atatatcgta aaaaacatct atgatttttt
    27121 acaagcttac acagaaaaag aaacttacac tgttaataaa cagtaccagt ttcgtctaga
    27181 taaacgggcg tttcaaaaag aagatcaaaa aatactcacg catcttgcag gaagtgctca
    27241 gacgcatcaa ttattaggta cgaacggctt acaagtcaaa ggaaaactag ataaaagata
    27301 tctgcttctc ccagtagaac aagcccgctt cctgttagag cagatgaatc agactacacg
    27361 tttgcatctg cagataaatg aacaaaacga tcaaggaata caattttccg atcaaaacaa
    27421 acctttatca ttcaaagtgg aaaaatttgg agaagattat ttgctgcaag cattgaatga
    27481 ttttgatttt ttctttatgc attatcagtg gggagttatc caagggacat tttattctct
    27541 gacgaaatat caacaaacgg tttatcaaac ttggaaacaa ttgattcgcc gattggagaa
    27601 accagaagta gtgttcaaga aaaaagaatt accgtctttg tttaaagaga ttatccctct
    27661 gctgtcagag atcggcgacg tagctattgc ccctgcagta gaagaggaag tagcagatat
    27721 ccccgtcgaa tttattttct ttttccgaaa agcaaaagga aaaatccaag ctcgagtcga
    27781 tttcgtctat ggcgaagtca tctattcgac tgacccgaag catgaggtct cgacagaaca
    27841 ggcttttcag gtgttacgtg acacagtaga agagaaacga gtacttgatc tattcagaat
    27901 gtatcgctac cagaaaaatg aaactggtta tgaacgtcca ttgccaagcg gggaagaact
    27961 atatgccttt ttccgcacag aactatctgt cttccgtcag tttggtgaag tcagacttgg
    28021 aagaaaattg cgagagttgt atttagatgc gcataaacat caaccgaaaa tcgaagtaac
    28081 cgagaccggt tcatggctcg atattcgatt cgatgtgaca gggatagagg agcaagaaat
    28141 cgatcatgtc ctgcaaagct tgctgagaaa tgatgctttt tatacattag aaaatggtca
    28201 ggttctttct tttgattcag aagaatttca acaaacaagc caagtgttgc aacaattacg
    28261 ggaatcgata cgtacagaag aaggaacgat ccatgtacct aaaaatcaag gattgatcat
    28321 tcaaaatcaa ttggaaaaaa gcaatgctac ttttagtgaa tcgttccaaa cgatggtaca
    28381 agatctcatt catccagaaa gataccaggc acagcttccc aaaggattga atgcaacgat
    28441 gagagattat caaaagcaag gttttcgatg gttgaagatg ttaggtcatt atcagtttgg
    28501 cggtatttta gccgatgaaa tgggacttgg gaaaacacta cagacaattg cctttttact
    28561 ttcggaaaaa gaagaacgga aaagcttctc tgctttgatc gtagcgcctg cgagcttaat
    28621 ttacaactgg caggcagagg tgagaaaatt cgccccttct ttaagtatcc aagtaatcaa
    28681 tgggaataaa aaagaacgtg aagaattact ggctaaagac acagatatcc gagtgacttc
    28741 ttatgccagc ttaagacagg atctagctaa ttatcagtcg caaaaaattg attatttgat
    28801 tcttgatgag gcacaaatgg tcaaaaatag cagtacgaaa actgcacaag cattgagaga
    28861 attagctgta cctcagcggt ttgcactgag tgggacacca atcgagaata atttagaaga
    28921 attgtggtca ttgtttgcaa cgattatgcc aggatttttc ccgactaaaa caaaattcag
    28981 ggaacttgcc gcagaagaaa ttgcgcaaat gattcgaccg tttattttac ggcgagataa
    29041 acaaactgtg ttgaaagact tgccggaaaa aacagagatg aatctatatt ctgcacttac
    29101 agaagaacaa aaaactgttt atttagctta cttgcgtcaa atgcgtgaag aaatcacatc
    29161 aatggattct gaagcattca agaaaaaccg gatcgggata cttgctggtc tgacaagact
    29221 gcgacaaatt tgctgcgatc ctcgtctgtt tattgaagat taccaaggcg gttccggcaa
    29281 gctcgaacaa gtaaaagatc tactggtagc tgccaaagag aacaaacgaa gagttttgct
    29341 gttttctcaa tttacaggga tgctgactat cttgcaagaa gagttagccg agctagggat
    29401 ctcgacattt tatttaagag gcagtacgaa accacaagat cggttaagta tggtagacgc
    29461 atttaatgct ggcgaaaaag atgtttttct gatttcctta aaggctgggg gaactggatt
    29521 gaatctcacg ggagcagata cggtcatctt gtatgacctt tggtggaatc ctgccattga
    29581 agaacaagct gctggacgtg cacatcggat cggacaaaaa aatgtggtag aagtgtggcg
    29641 tatgatctct gaaggaacga ttgaggaacg catggattca ctccagcaag aaaaacgcga
    29701 actgttccaa aaagtgatcc aaggtaatga agaacagttg acgaaactga ctgaagaaga
    29761 catccgcatg attttaagtg taggagaaat cgaggagtaa gagactcgaa aaagcgttca
    29821 gtaatgggta agaaagcctt tctaaagaag cgattgttta tttgatttaa aaaatagcaa
    29881 ggaggctggg attccatttt atcccagctt ccttgctatc atttttttat ttctattttt
    29941 aaaggttata caaagattgt cttcattgtc tctaacagct gatcaaaaga agaaatctcg
    30001 atatcgcaaa ttggagtctg ttcaattttg cgttctcgat ggttgaacca aagcgcttgc
    30061 cagttagcac tttttgcacc tagaacatca ttgtcgtaat tatcgccaac gtataaggtc
    30121 tcttctggaa gcatatgaaa ttctttctca gctagctgaa aaatctcttt ttctggtttt
    30181 tggaaacctg ttgcttgaga aatgagcata ttgcgggaag gaatccaatt gttcaattga
    30241 agttgcttca gtttttttgt ctgatggtca gtcggcccat ttgtgataat tcctaatggg
    30301 acattctttt ccttcaagaa atcgagggtt tttttgacct cctcatgcaa gcagatatta
    30361 tcaagttctt cctcgtaaat tttttgaaat aagaggccat cttcttccgt aatatgaggg
    30421 tagtctaaat cttttagtga ttggctgatc cgatgtgctc gcatatattc tagtgtccaa
    30481 tctcctgcca ttacttttgg gaagttttca tcactatgat ggcggaaccg gatatatagt
    30541 ggatgcatat ctgcatctga aacaagcgga acgactcttt tgaccgcgtt tcgaaatggc
    30601 tgctgctgat catacatcgt atcatcaaca tcaaacacaa ctgatttaat caaaatttta
    30661 cacgcctttc ggttttcatt gattttttca caaatccttg actattgtaa cagaaaaaat
    30721 tgtctaaaaa agctgataaa taaaggtttt tcttactata tttctatttc cttgacagaa
    30781 taaggttttt atatttcagt ttttttcact aaaaatcgtg aaacacgtca ataagtttga
    30841 tgaacaaatt aatcattatg gtagaagtcg attgatttat tttaatgcaa tctctgtgga
    30901 attttcgaaa g
//
LOCUS       DAZBTF010000028        30017 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000028 DAZBTF010000000
VERSION     DAZBTF010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30017
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.028"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..2424)
                     /locus_tag="ACHGNS_001345"
     CDS             complement(61..2424)
                     /locus_tag="ACHGNS_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HFN6937302.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            2589..3093
                     /locus_tag="ACHGNS_001346"
                     /pseudo
     CDS             2589..3093
                     /locus_tag="ACHGNS_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3201..3548)
                     /gene="rbfA"
                     /locus_tag="ACHGNS_001347"
     CDS             complement(3201..3548)
                     /gene="rbfA"
                     /locus_tag="ACHGNS_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFN6937303.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(3575..5881)
                     /gene="infB"
                     /locus_tag="ACHGNS_001348"
     CDS             complement(3575..5881)
                     /gene="infB"
                     /locus_tag="ACHGNS_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFN6937304.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(5894..6205)
                     /locus_tag="ACHGNS_001349"
     CDS             complement(5894..6205)
                     /locus_tag="ACHGNS_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFN6937305.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(6202..6495)
                     /locus_tag="ACHGNS_001350"
     CDS             complement(6202..6495)
                     /locus_tag="ACHGNS_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFN6937306.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(6517..7692)
                     /gene="nusA"
                     /locus_tag="ACHGNS_001351"
     CDS             complement(6517..7692)
                     /gene="nusA"
                     /locus_tag="ACHGNS_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFN6937307.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(7716..8189)
                     /gene="rimP"
                     /locus_tag="ACHGNS_001352"
     CDS             complement(7716..8189)
                     /gene="rimP"
                     /locus_tag="ACHGNS_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFN6937308.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(8333..12685)
                     /locus_tag="ACHGNS_001353"
     CDS             complement(8333..12685)
                     /locus_tag="ACHGNS_001353"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFN6937309.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            complement(12897..14606)
                     /locus_tag="ACHGNS_001354"
     CDS             complement(12897..14606)
                     /locus_tag="ACHGNS_001354"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFN6937310.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            complement(14674..15942)
                     /gene="rseP"
                     /locus_tag="ACHGNS_001355"
     CDS             complement(14674..15942)
                     /gene="rseP"
                     /locus_tag="ACHGNS_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFN6937311.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(16103..16903)
                     /locus_tag="ACHGNS_001356"
     CDS             complement(16103..16903)
                     /locus_tag="ACHGNS_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFN6937312.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(16900..17712)
                     /locus_tag="ACHGNS_001357"
     CDS             complement(16900..17712)
                     /locus_tag="ACHGNS_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFN6937313.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(17907..18464)
                     /gene="frr"
                     /locus_tag="ACHGNS_001358"
     CDS             complement(17907..18464)
                     /gene="frr"
                     /locus_tag="ACHGNS_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFN6937314.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(18467..19189)
                     /gene="pyrH"
                     /locus_tag="ACHGNS_001359"
     CDS             complement(18467..19189)
                     /gene="pyrH"
                     /locus_tag="ACHGNS_001359"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFN6937315.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(19325..20206)
                     /gene="tsf"
                     /locus_tag="ACHGNS_001360"
     CDS             complement(19325..20206)
                     /gene="tsf"
                     /locus_tag="ACHGNS_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFN6937316.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIEYDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(20305..21087)
                     /gene="rpsB"
                     /locus_tag="ACHGNS_001361"
     CDS             complement(20305..21087)
                     /gene="rpsB"
                     /locus_tag="ACHGNS_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFN6937317.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            21446..21925
                     /locus_tag="ACHGNS_001362"
     CDS             21446..21925
                     /locus_tag="ACHGNS_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFN6937318.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            22142..23233
                     /gene="phnW"
                     /locus_tag="ACHGNS_001363"
                     /pseudo
     CDS             22142..23233
                     /gene="phnW"
                     /locus_tag="ACHGNS_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
     gene            23226..24011
                     /gene="phnX"
                     /locus_tag="ACHGNS_001364"
     CDS             23226..24011
                     /gene="phnX"
                     /locus_tag="ACHGNS_001364"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050194 - phosphonoacetaldehyde hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFN6937319.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(24357..26048)
                     /gene="argS"
                     /locus_tag="ACHGNS_001365"
     CDS             complement(24357..26048)
                     /gene="argS"
                     /locus_tag="ACHGNS_001365"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFN6937320.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(26470..27417)
                     /gene="arcC"
                     /locus_tag="ACHGNS_001366"
     CDS             complement(26470..27417)
                     /gene="arcC"
                     /locus_tag="ACHGNS_001366"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFN6937321.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(27532..28551)
                     /gene="argF"
                     /locus_tag="ACHGNS_001367"
     CDS             complement(27532..28551)
                     /gene="argF"
                     /locus_tag="ACHGNS_001367"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFN6937322.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(28642..29871)
                     /gene="arcA"
                     /locus_tag="ACHGNS_001368"
     CDS             complement(28642..29871)
                     /gene="arcA"
                     /locus_tag="ACHGNS_001368"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFN6937323.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         8008 a         6455 c         5345 g        10209 t
ORIGIN      
        1 cttaatttga caaataatac aatgtaaaaa attctgtttt gcgggatcga ttttatcatt
       61 ttattggtca agatttttcc atttccaatc caatacttct ggcagatcaa taccattttc
      121 acgaatatag ttttgatgtt cttgtaattt ctgatccatt ttttccgcaa aatcttctgc
      181 ttgacttcct ttgattttta aagctgcgtc ttttgccaaa tgataacgat caagttcatt
      241 tactacacgc atgtcaaatg gtgtcgtgat atctccattt tctttgtagc catgaatcat
      301 taaatgatgg ttatgacgat caaagaaaat cgtgcggacc aatgtttcat aaccgtggaa
      361 agcaaataag atcggtttgt cttttgtaaa atattgatca aattcttcat cgctcaaccc
      421 acgcggatct tgacttggat gacgtaattt caataaatct acgacattga tgaaccgaat
      481 ttttagtttt gggaattctt ccagcagcaa agttacagct gctaatgctt ctaagttaga
      541 ttccgttcct gcagcagcaa tgaccaattc aggttcctcc tcttttgctg tcgaagccca
      601 atcaatgatt ttcaagcctt cattcactaa gacggctgct tcttctgctg agtaaaattg
      661 agcacgcgga tgtttggaag agatgatcaa attgattttt tctgacgagc ggaatgcttt
      721 atccatgact gcgagcaatg tatttgcatc cgcaggcaga tattcacgga tgaactccgc
      781 cttcttttct gccaaatgtg tcaatattcc tggatcttgg tggctatatc cattgtgatc
      841 ttgctggaag actgttgagc ttgcaatgag attcagcgaa ggataatctt ttcgccaaga
      901 aaggtcatgt gatttccgta tccatttgaa gtgctgagtc aacatagagt caaccactcg
      961 caagaatgat tcgtaacttg caaaaaaacc atgccgtcca gtcagaacgt acccttctaa
     1021 gaacccttca tcttggtgtt ccgataattg actgtcgatg acgcgaccag atggcgaaag
     1081 ccattcatca ttttctggtt cgattttttc catccattgg cgatttgtcg ttttgaaaac
     1141 ttgattcaat cggtttgatt ttgtttcatc tggtccaaat atacggaaat tttctggatt
     1201 tttctcaatg atctcgcgaa cgaatttacc aagttcggtc atatcttctg ctttaacgga
     1261 gcctggtttt tcaaattgca aagtatattt tttccaatct ggcataataa gcggtcttgg
     1321 atcgattcca ccattggtga taggattttt tgccattctt tggtcaccag taggcgcaat
     1381 ttctctgatt tcttctaaaa tacgtccttg tgcatcaaat aatttttctg gttcatagga
     1441 tttcagccaa cggagtaaag catctgcatg atccatatgt tcttgatcta caggaatcgg
     1501 tacttggtgt gctcggaagg ttccttcaat tggttccccg tcccattctt ttggaccggt
     1561 ccatcctttt ggtgtgcgta cgatcaatac tggccatttt ggcatcacga catctgttgc
     1621 tgtcttcagg cgagcttctt tttggatgga tttgattttc tcgattgctt gatccatttt
     1681 ttcagccata acaggattta atttttctgg atcatttcct tcaataaaga aaggctccca
     1741 gccaagtccg ttaaagtaat ttgctaattc tccatctgat ttacgtgcca agatcgttgg
     1801 gttggctatt tttgcaccat ttaaatgaag gatcggtaaa acagctccat ctatcactgg
     1861 attgataaag acatttgaga accagcttcc agctaaagga ccggtttctg cttcaccatc
     1921 accaatcacg acagcagaga ttacttcagg attgtctaat acagctccga ctgcatgtga
     1981 aagagagtat ccaagctctc cgccttcatg gatagaacct ggtgtttctg gtgctgcatg
     2041 agaagcaatg cctccaggaa atgaaaatcg cttgaatagt ttttgcattc ctgtctcatc
     2101 ttccgttact tctggataga tctctgtgta acttccatct aaataagcat tcgataccat
     2161 gacttgtccg ccgtgtcctg gcccttcgat ataaaacatg ttcaaatcat atttattgat
     2221 gactcggttc aaatggacat aaataaaatt ttggccagga atcgttcccc aatgaccgat
     2281 tggatgtttt ttgacatctt ctggtttcag tgtacgtctc agtaaggggt tatctttaag
     2341 ataaagttgc ccaacagaaa gataattagc agcacgccac caagccgtca ttttatcaaa
     2401 gtattcttta ctagaataat ccatacataa cactcctttt cattattact cttattctag
     2461 cgcagatact cccaattgaa acctaataag cacttctgtt catttgtttg gattttatat
     2521 ctagccaaat gaacaggtat aaaaaaccac gaaatttttc ctttcgctga cgttttttac
     2581 attgatccaa aaaacttgtc gttatcacaa tggctgttcc gcagttcatt tcgctcctca
     2641 tcatgcataa tttgctgaat gacgctaggc cagttaacac attgttggaa aactggggct
     2701 ggatcgagca attcctttcc tgattgatcc gttttatgta aaagtctctg ttatctttgt
     2761 caatatgtga attggtatcc cagctactat gctaatttct acagggatta tccaaaattt
     2821 ggatcgtgac caaatcgaag cggcgtaatc ggaaatatca ataacttcaa cgtgattttt
     2881 cttttaactg tcaacggacc gacaaactca aacttttatg gtgcaggttc cactgatctt
     2941 ctagttactt ggctatacct gactgtcact aaaatagatt acaatctagc ttccgttatc
     3001 ggtatcttga tctttatcta tatgcaaaaa tttatatact atagaaaaaa gaaagtcgaa
     3061 ttttttactt ttctttttct cttttttggt tagctgacac gctaccagac taaagtcatg
     3121 gaactcttag gtaatacgtc ttaatgaaaa caaaaaaatc ccgcaacccc tgatttcaca
     3181 ggatttgcgg gattttttga ttagtctttg ttcaagtcgc gaagcaattc atcgatatgg
     3241 ttcccataga caactgattc atcaagttca aagaacaatt caggtgtctt ataaatactt
     3301 agattgtgcc ctaactctcg acgaatcaat cctgatgctt tattcaatcc atcttgtgct
     3361 tttttcttat ctgatgctaa gtcagataaa atgctgtaat agatcgttgc ttgctgtaag
     3421 tcccccgtaa ctttcacatc tgtgatcgtt acgttctcca cacgagggtc acggaccttt
     3481 ttgcgcaaaa tatcattgac ttcttttaag atttcttgac cgactcggcg atcacggtag
     3541 ttagccataa tgattggcct ccttttatca gtttttagtc atttttgact tcttccatga
     3601 taaatccttc aattacgtca tctactttga tgtcattgaa tttttcaatc atcgcaccac
     3661 attcataacc cattttgact tctttcacgt catctttgaa tcgtttcaga cttgcaagtt
     3721 ggccttcata gattacgatt ccgtcacgga ttacacggac accgctatct ctacgaatat
     3781 atccttctgt tacataggca cctgcaatcg taccaacttt agaaactttg aaggtttcac
     3841 gaacgatcat ttgaccggtg attttttctt caaattcagg gtctagcatc cctttcatcg
     3901 cagtttcgat ttcttcgatg gctttataga tgatacggtg aagacgaata tcgacttctt
     3961 cagtatcagc ttgcactttt gcctgcggtg taggacgaac gttgaaaccg atgataatag
     4021 cgttgctggc tgctgctaag gttacatcac tttcattgat tgctccgaca gctgaatgga
     4081 cgatttttac acggacacct tccacatcaa tcttcttcaa tgatgcagcc agtgcttctg
     4141 ctgaaccttg tacatctgct ttgatgatta cgttaacttc tttcaattcg ccttctttca
     4201 ggctttcaaa taagttgtcc aaagtcacac ggcttgtggc agcacggttt tccaataatg
     4261 cacgttttgc gcgttcttca cccgctgcac gtgctgtctt ttcgtcttcg aagactacga
     4321 aacggtcacc tgcttgaggt acatcattca agccagtgat ttctactggc gttgcaggtc
     4381 ctgcttcttt atcgcgacgg ccaagatcgt ttgtcattac acggacacgt ccgtaagtat
     4441 taccgacaac gattgggtca ccaacgtgaa gtgttccttc ttggacaagt aaggttgaca
     4501 caggaccttt acctttatca agacgtgctt cgatcactgt tccgattgcg cgttgtgttg
     4561 gatctgcttt taaatcttct acttctgcga ctaacaggat catttccagc aattcttcga
     4621 tgttttggcc gaatttcgct gagatctcaa caaaaatcgt ttctccaccc catgcttcag
     4681 gaattaattc atactcactt aattcttgca taacatgctg aggatttgca cctggtttat
     4741 caattttatt tacagcgacg atgattggaa cgcctgcagc tttggcatgg ttgattgctt
     4801 caactgtttg tggcatgacg ccatcatctg cagcaacaac taagatcgtg atatctgtga
     4861 tacttgctcc acgtgcacgc atacttgtaa aggccgcatg tcctggtgta tccaagaaag
     4921 tgattggttt accatcaata tcgatctggt aggcaccgat atgctgtgtg attccgccgg
     4981 cttcgccgct tgttacacgt gaatggcgca atgtatccaa taaggttgtt ttcccgtggt
     5041 ctacgtgtcc cataatcgtt actactggcg gacgtgagac taatttatct ggatcgactt
     5101 cttctgcctc gaagaattta tcgatatcag caatgtcaac ttggactttt tcttgtggtt
     5161 ccatgccata gtccgtcgct aacagttcaa tcgtatcttt atccagtgct tggttttgat
     5221 tgaccatgac accaagcata aataattttt taatgatttc tgctggctca cgatgaattt
     5281 ttttcgcaat atctgctacg ttcatacctt cagtatactc taatacatct ggcaattcac
     5341 ggaatttgcg tggtggtaca gctggtttag ttgattgttg ctgtttgcct tttttacctt
     5401 ttttattata acgattacgg ttattattat taaatttgcc gcgattgtta ttgtttggtt
     5461 tgttgttatt gttagtacgg ttttgattgc cgccttggct cgttccttga cgattttggc
     5521 tgctggtatt tcccgtatta ttttggtttg ttgttggttt ttttccttga ttcacttgac
     5581 cgctcccttg accgcgatct tgataactgc ggttattttt atttttttgt tgattgttcg
     5641 ttgtttcttt tttacgttga tcgttcggct gttgattggc tggttttctg cttgcttgtt
     5701 gtgcagcttg ttgtttcgtg cttgggtttt tgaacgcttg ttgcaatttc ttctcatcac
     5761 tgctagtaat cgcacccata tggtttttca catcgatgcc aagttgttgt gctttttcca
     5821 cgacgtcttt actagatttt ttgagttctt ttgctaattc atagattctt ttgttgccca
     5881 tgcaatcacc ttcctaacct tcaattagct ccttggtctt cttcgcaaat ccggcatcca
     5941 ttattccgaa taccttgcga ggttttccga tcatctgcgt gatttctgct tcagaaaata
     6001 gctcaaagca aggaacttca taatatgaac tcttatcttt tatctttttt cttgtattct
     6061 cacttgcatc ggttgccaca aacaccaatt ttgctttttg tttgcggatt tcagcaatgg
     6121 tcagttcttc acccgtgatt agttttcccg cgcgcatcgc tagaccgatg agattcatgg
     6181 ctttttgccg attaactgta ttcattttcc aaataactct ttacgtgctt tttggtgagt
     6241 gacatagtca agaagttctt gataaaactc atctgttaat gttgtttcga gtactcgatc
     6301 gagaattttt ttatcccaag cttgttgtac ttcttccggc tcaagtgaga catacgctcc
     6361 tcgtccaggc atttttccag ttggatcgat cgatacttcc ccttcttttg aacgggtgat
     6421 tcggaccatc tcttttttag gtttcatttc acctgaaacc acggatttgc gtaaaggaat
     6481 tttcctttgt ttcatctctt tgcctcctta ctaaagttaa gcttctggtt tttcttcgac
     6541 cgtttcattg ttgaatgcaa ttgtttcata ttcatcgtct gttaaatctg actgaacgat
     6601 tgcttcatca tgcagttctt ctgtttcttt ttgttgttct tgattttcat aaaattccgt
     6661 catgtcagat tctgatttga tatcgatttt atggttagtc aatttagcag ctaaacgtgc
     6721 attttgtccg cgtttaccga ttgctagcga taattggtag tctggaacga caactgtgca
     6781 ggcctttgga tttgtctcat cgaagatcac atccatgact tgtgcaggat tcaatgcatt
     6841 tgcgatgaat acagctggat cttcattcca ttcgacgata tccatgtttt cgccttttaa
     6901 ttcattgacg attgcttgaa ctcgttgtcc ctttggcccg acacatgtac cgactgcatc
     6961 gatatttgga tcatttgagc gaactgccac ttttgaacga tcgccagctt cacgggcgat
     7021 gctgacaatt tcaacgattc catcatagac ttctggtact tcttgttcaa ataagcgacg
     7081 caataggtct ggatggctgc ggctaacaaa tacttgcgga cctttagacg tattttctac
     7141 tcgggaaaca taaactttga ttcgatcatg tggttgataa aattcatttg gcatttgatc
     7201 ttgtttagaa agaactgcct caattttacc aagattgaca taaatataac gtttatcctg
     7261 gcgttcgacg attccttgca tgatgtcttt ttcataagca ctgaattcgt tataaataat
     7321 cgttctttct gcttcacgta cacgctgtaa aataacttgt ttagccgttt gcgcagcgat
     7381 ccgtccaaaa tcttttggtg ttacttcaaa acggatcgta tcaccgatct catatgctgg
     7441 attgattaaa agtgcatctt tcaatgagac ttccaactgt gaatccatca cttcttccgt
     7501 tacttctttt accgcataga catggatctt tccttttttc tgatcaaatt ccacttcgac
     7561 gttttgtgct tggccatagt gacgtttgta tgcagaaacc aatgctgctt ctaaagcatc
     7621 gatcacgatt tcttttgaga tccctttttc agcttctaaa gcgtctaacg catttaacat
     7681 ttctttactc atttttttct gattcctctc cgtgattaaa attggatagc taagcgagct
     7741 tttgcaatat tttttcggtc aaaagtcaat tctttttctc gtgtttttat gcgtattttc
     7801 aatgtaagct gttcagcatc aaatgattga agaaatcctt catattgttt ttcgccatct
     7861 actggttggt ataaggagac atggacataa tcaccaagtg ccttttcata gtcgctttct
     7921 tttttcaatg gccgctctgc tcccggggag gatacctcca ggaaataagc ttgaggaatc
     7981 ggatctggat ctgctgcatc aagtttttca ctcagtttct cactgacaaa ggcgcattct
     8041 tcgatatcga taccgccttc tttgtcaata aatactcgta agaaccagct ttttccttct
     8101 tttacaaatt cgacttcgac aagttcaaaa ttttgttcat ctaaaatcgg tgtcaccatt
     8161 tctgtgactg tttcaacgac gctactcaaa agtttcgcct ccttaaaaat taagtgcgta
     8221 attccattaa aaagagtgag cgaaaaatcc gctcactcac agttagtatc attacctata
     8281 agaactatac catactctgc ttaaaattac aagcagacag gaaaacagat gcttatagca
     8341 tatcgaataa cgataattga ttttcatctg gtagatcttt caatacgcca ttgtcgttca
     8401 tatattcgat caatgttttt gaaactttac cgcgggtggc aagatcttct ttggataaaa
     8461 atggaccgtc ttttcttgct tcaacgattt gtttcgccac gttggttccc aaacttggga
     8521 cagctctaaa tggtgcgatc aacgtatcac cttcaataac aaagttaacg gcatccgatt
     8581 tatacaaatc aatcattcca aatttgaatc ctcgttccag catttcattg gctaactcca
     8641 gcactgtcag ttgattcttt tcttttacgg aagcatctaa tcccttgtcc gtgatttctt
     8701 tcattgcttg tttcacagca tcttttcctt tgcacatgga gactagatca aagtcgtctg
     8761 cacgtacaga aaagtatgca cagtaataca aaatagggaa ataaaccttg aaataagcga
     8821 cacgaagagc catcaatacg taagctgcag catgggcttt cggaaacata tatttgattt
     8881 ttgaacaaga atcgatatac cagtcgggca cattattttc tttcatcgcc gatagatagg
     8941 tttctcgcaa ttcatcaggg atcttgttcc attgtccttt acgaacagtt tccatgattt
     9001 taaatgccat cccgctatct aatccagcat ggatcaaata aaccataata tcatcacgac
     9061 agccgattac ttccgccagt gtcgcatctc ctcgtcgaat caattcttcc gcatttccta
     9121 accatacgtc tgtaccatga gatagtccgg aaatttgcag tagttctgca aaagttgtag
     9181 gatgcgtttc ttctaacatc cctcgaacaa aacgagtgcc gaattctgga atacctaatg
     9241 tccctgtttt ggaataaatt tgttcctgac ttactccaag tacttcagga ccggcaaata
     9301 tccgcatcac ttccgggtca tccgtaggga tcgtttgtgg gtcgatccct gataaatctt
     9361 gaagcatccg aataacagtt gggtcatcat gccctagaat atctaatttc aatacattat
     9421 cgtgaatcga gtggaaatca aaatgagtcg ttttccattc tgaattttga tcgtctgctg
     9481 gatattggat cggcgtaaaa tcatagacat ccatataatc gggaataacg atgatccccc
     9541 ctggatgctg gccggttgtc cgtttgactc cagtagcgcc ttttgctaat ctgtcgactt
     9601 ctgcacttcg aaactgcaga ttattgtccc tttcgtatcc ttttacatag ccatatgctg
     9661 ttttgtcggc gactgtacca atcgttccgg cacggtaaac atagtcttcg ccaaacaata
     9721 ctttcgtata gttatgggct tctgcttgat aatcacctga gaagttcaaa tcaatatcgg
     9781 gcactttgtc tccatggaat cctaaaaatg tctcaaatgg aatatcatga ccgtctttgt
     9841 ttaagcgtgc accacatttt ggacattgtt tttctggcat gtcaaatcca gaaccatacg
     9901 taccatcttc aaaaaactcg gaatattggc actctggaca ataataatga ggggctaatg
     9961 gattaacttc agtgattcct gtcatcgtcg caacaaagct agagccaaca gagccacgtg
    10021 aacctactag atagccatct tcattacttt tatgcactag tttttgggcg atcagataaa
    10081 taaccgagaa gccattacca ttaatagaat tcaactcttt ttctaatcgt ttttgaatga
    10141 tctcaggtaa cggatctccg tacatttgtt ttgcttttgt gtaactcaat tctgatattt
    10201 catcttctga acctggaatc ttcggtgtat aaagttcatc tttgactgga atcacttctt
    10261 cgcagatatc tgcgatttta ttcgtatttt ctactacgat ctctttcgct gtttcttccc
    10321 ctaaaaagtg aaatgcagtt agcatctcat cggttgttcg gaaatggaca tccggcaaac
    10381 tatgacgatt caacggattc gctccgccca tagaattgat caaaattttt cggtaaatgg
    10441 catcttcttc gttcagataa tgtacattac ctgttgcaac aacgatttta tccaaacttt
    10501 ttccgatttc taccaaattt tggataattt cttccaaatc atgttcattt tttaccaatt
    10561 cttgctcgat caaaggcgca tagaccgctt ttggcataac ttcaatataa tcatagaatt
    10621 ttgctcggtt tcttgcctct tctacccctt tttgcatcat ggcttcgaat atttctccct
    10681 tatcacaagc tgagccgatc aatagatttt cacgcatctt tttcaattgg gagcgtggta
    10741 ttcgtgggac gcgttcgaag tattcgacat tcgacattga aatcagtttg aacaaatctt
    10801 tcagtcctgc ctgatttttt gctaaaatcg ttacatggaa aggtcgagcc cttttgtaag
    10861 aatctccttc tccgatatgc tcatttagct gatcatgata aagcatgtta tgattgtcca
    10921 ttgcctcttt cacaaagatc catgctaagt gaccagttgc ttcagcatca tagattgctc
    10981 ggtgatgctg ttccaagcta acaccaaact ttttagataa cacacctaat ccgaaacgtt
    11041 tgaattgagg atacagatat ctagctaatt ccaacgtatc aatgactgga ttggccgctt
    11101 caggaatacc ataccttgcg tagctagtat tcaagaaccc catatcaaaa gccgcgttgt
    11161 gggctaccaa aattgtatct tttgaaaatt caaggaacat ccgcaatact tcttcttccg
    11221 actttgaccc tcgaaccatt tcatccgtga taccagtcag atctaccgtt gttcgtgaca
    11281 atggatgtcc tggatcgata aattcatcaa agctttcgat cacatttcct ttatacattt
    11341 tgactgcggc tagctcgatg atcgtgtcat atactgcaga caatcctgtc gtttccacgt
    11401 caaatacgac gtatgtggct tcactcaatg cttcatgtgc atcattataa gcaattggga
    11461 caccatcatc tacgacattc gcttccacgc cgtataagat tttcacacct gctttttttc
    11521 cagcactatg tgcttccgga aaggcttgcg caccaccatg gtctgtaatg gcgatagctc
    11581 ggtgtcccca ttttcctgct tgtgccacaa gatcagagat gctgtttgtt gcatccatcg
    11641 tactcatatt gctatgaaca tgaagctcta ctcgtttttc cccttcagga gcataatctt
    11701 tgcgaggtgt atgtttgact tctatgatat cttgcgcatt catgaccaaa tcacggacaa
    11761 acgtatcttc ttgaatactt cctcgtacct ttaaccagct accgacactg atcgcatcaa
    11821 atacttgttc atctttttcg ccgttcgaga attttttgac gataaatgat gaagtataat
    11881 ctgtgatttt caaagtcaag atcttgcgtt ttgaacgtag ttctctgact tctttgtcga
    11941 atacgtaccc ctcaagtgtg actcttcgtt cttcttcaac gatactgatc attggtgtcg
    12001 ttggttcatc ggctggaata tttcgtccta gctggatggg tccttcaaga gctggcgatt
    12061 gctcttttct ttcctttttc ttttgttcgt ggacaactag actttcagct gcttgcttca
    12121 taaaggcttc tgcctgttct tgtttgcgtt cttcaaaaag tttcaataca cgttccgctt
    12181 gctgctcatc cacttctggt tcgatacgaa attttgggaa tccgtaactg acgaacaact
    12241 cttctactaa tggcaagtat tgttgcttca aataagaaat agctcctgta ctgtcgattg
    12301 gcaagatgac tttacgatct tttatcacag gtacctgcgt tttcaatact tgctgtgcta
    12361 gtggtgtatc gcattgctga ttttccaaag cttgggccca gtaatcttgc aagagctgtt
    12421 catcgaatgt ctggtcatct gctaagatat tcagtttgac ttgtgcaata tctttaaaag
    12481 cgatagtcag atgctgagtc aatgaccgat atagcataat aggcaaaatt ttggaaaaat
    12541 gtagcgtaaa ttcccataaa cgcgatttac gatggaccac tacactatcc atttcaccag
    12601 cagtaagcag tggatgattt ttttctgctt cctctagttg gatttgttcc aacagttttt
    12661 caaataaatc tcgtttttct gacaaagata tacgcccttt ctgatgagaa acaatgcaat
    12721 tgatcgcatc ttatcctctt ctttttcagt accttttagt atactcgttt tttctgagtt
    12781 ttacattaaa aaattggaac tgcgattcaa atcgcagttc caattttaaa agcgaatcat
    12841 cagattgtca aaaacagctg attcagctat ttttattttc tgtgttttaa cgcttattat
    12901 tcgccttggt tcaagaggat tggtaaagta ttcgccaatt cttctttacg tacttcaacc
    12961 atttcgccag ttttcttgat tttcacttct acgattccat ccactgcttt tttaccgaca
    13021 gtgattctga ttggacagcc aatcaaatca gaatccgcga atttcactcc tgcgcgttca
    13081 tttctgtcat caaccagtac ttcatagcca gcttttgtca tcgcttcttc tagttcattc
    13141 gtcaggcttg tttggtattc gtcttttaga ttcatttgga cgatatgcag atcaaatggc
    13201 gcaattcctt ttggccaatt gattccttgt tcatctgaat gttgttctac gattgctgat
    13261 aataaacgac ttacaccgat accataacag cccataatca caaatttttc tcgtccattt
    13321 tcgtctagta cagttgctcc catagattca ctgtaacgag tgccgatttt gaatatatgt
    13381 ccgatttcga ttcctcgagt aaaggcaaga acaccgttgt tgtctggtga aggatctcct
    13441 tcttgtacaa aacgaagatc ttcataagaa agcacagtga aatcgcgttt tggattcaca
    13501 tttttatagt ggaatcctgt ttcgttcgct ccagtaatgg cattcgctaa atcttggaca
    13561 taacggtctg catatacttt tacgtcatct gaaacaccga ctggtccgac agaaccaaac
    13621 tcagcaccta gatatttttg tgcttcttct tctgttgctt cttctaaaaa gtcagcagat
    13681 aaaaagtttt tcagttttac gtcattgact tcatggtctc cacgtaccaa aacaaggaca
    13741 ggttgttcat ccgcaatgaa caatacagac ttgatgattt tttgaggttc aacagaaaag
    13801 aaatctgcta cttcgtcaat tgattttacg ttaggtgtag ctactttctc caattcaagc
    13861 tgtgtttcat gcgatttttt tgatacatag tagcttgtag ccatctccaa gtttgcagca
    13921 taatcacttt cagtcgaata acagatcgta tcctccccga tttctgagat cgccatgaat
    13981 tcctttgagt ctttcccgcc cattgcgccg ccgtctccga tgatcgagcg aaattctaag
    14041 ccacaacgct tgaatacttc ggtataggct ttttcgtaat ctttatatgt ctcgtccaaa
    14101 ctttcttccg ttgcatggaa agaatatgca tctttcatga taaactctct tccgcgaagc
    14161 aaaccgaagc gaggtcgttt ttcgtcacga tatttaggtt ggatttgata aagatttaaa
    14221 ggtaatttct tatatgaatt gatttcatta cgcatcaaat cagtaaaagt ttcttcgtga
    14281 gttggtccaa ggatcatttt tcgatcattg cggtctttga aacgatacaa gtttggtcca
    14341 tatgtttcgt aacgcccgga ttcttcccaa agttctgctg gcaagatggc aggcatcagc
    14401 atttctaccg ctccgatttt ttcaaattct tcccgcataa tgcgtttcag tttttccaat
    14461 acacgatttg ctaatggcag atatgaataa atacccgctg atacttggcg gatatatcca
    14521 gctcttagca agatttgatg gcttaatact tctgcatcat ttggaacttc gcgtaatgtt
    14581 ggaatcaaca tttttgattg tctcattcta tatactcctt tatttcctca aaatagttat
    14641 cgtttgttat tataccctat ttgtcaactt tttctagaaa aagaatcgtt gaatatcgtt
    14701 ccatgtaact agcaccatca acaacattaa aaatccaaag ccagctaatg tcaatattcc
    14761 ttctttttct tgacttaatg gtttcccgcg aatcccttca aaaatattca agactaattt
    14821 cccgccatct aaggcaggaa tcggaagcag attgacgatt ccgagattca ttgaaagaag
    14881 cgccatcaaa ctaataacag ttgttacacc ttgatttgct gcttctgaag acaattggta
    14941 catcatgaca ggtccgccta atttatctaa actgaaacct gtaaatagtg atcctaaggc
    15001 tttgaaaatt tccaatgaac cgctgaaagc ttgacgcgtc cctccaatga tcttatccat
    15061 aaaacctgta ttcattgggg cttttatacc aagttggcca actttttcgc catttgactc
    15121 tacagatttc ggtgtcacgt cgacagattg cacctgacct tctcgttcga tcttaaagtc
    15181 taatgtttta cctggatttt tcgtaatgac tgttgtcaga tcattccatg aatggatttc
    15241 ttttccgtcc acactgacta cctcatcatt ttcttttaag cctgcttctg ccgcagctcc
    15301 attaggcatg atctctccta cgcgattagt gttcgtcact tgaacaccac cttgcataaa
    15361 tgccagaatg atgaataaca cgattgccaa aataaagtta ttcatgggtc cagcaaagtt
    15421 ggtcagcata cgctgccaca atttagccga ttggaactgt acgtcttttg gcgcaattct
    15481 gatttctgtt ccgtcgtttt caataatcgt cgcatcgtga tcaacagggt aggttactac
    15541 ttctgtttcg tctccattca cataaccagt gatagtcagc tcatcttcta aatcataacg
    15601 gctcaattcc atcgggatag catttgtcaa ttggattttc ttacttaaat ttatcttctc
    15661 tacgatgccg tcagaattta gtaaaagaga aagaggcatt cctggtgcca tttctgtttc
    15721 atcatcccca ttgcctgcca ttcgaacata accgccaata ggtaataggc gaagcgtata
    15781 tgttgtcccg tcttttgctt gatgcccata aattttcgga cccataccga tagcaaattc
    15841 gcggacgagg attcctgatc gtttcgcaaa gaagaaatga ccaaactcat gaacaatcac
    15901 taatatacca aaaacgatga taaatgtcag aatcgttttc atagcttttt tctcctttac
    15961 ttgttttttg tgttttttat aaaacaacga taaaacacta taattttacc atagtatacc
    16021 aaagacacca atagtatcaa tataatgaaa aataaaaacc tgcgtctttt atgaaattca
    16081 caaagaaacg caggtatact tactaaataa tgccaaataa atgcatgata ggaaatacaa
    16141 acagcaaact gtcgaaacgg tctaagattc caccatggcc tggtaagata tttcccgagt
    16201 ctttcacatc ataatgacgt ttgattgcag attcgaccaa atcaccaaat tgtccgactg
    16261 ctgaaaaaat aatcgtcatc agaagcatga caaacatacc atgattgaat agttcttttg
    16321 ctgggtatag caataaataa agaaatgcga ctacaacagc actaccgata ccaccgagcg
    16381 caccttcaat cgttttgttg ggcgaaatat caggacttaa cttatgctga ccataacgtc
    16441 gaccaaccat atatgctccg atatcagttg cccaaacaat aaatagacca aatatcaaaa
    16501 cagccaagct ttctgttcgt gcattgacaa aattctggaa acctattccc acatacaagc
    16561 tgacaagcac aggaaaacct gcttcatcaa tcgtatacgt atttttagaa ataactgatg
    16621 ttcctaaaag aatcataact ataagataaa ataacataaa tgtacttaca ttctctggaa
    16681 gaaagaaaaa ccatctttct tttggcaaaa caagtaacac tgcaccaacg gcggataaaa
    16741 ctccttcaaa acttaagagt gtcaatcctt tcatccggaa cagctcgtaa actcctacta
    16801 ccgctaagat tgctgctgta agctcaaccg ctatgcctcc gatccatata atcggaataa
    16861 acaagactag cgccacagct gctgtgatca cacgttgttt catgactgat cccctttctc
    16921 atcttctgtt ttttttacac cgccgaatcg acggtctcga ttttgataag aggcgattgc
    16981 ttcttcaaga tgcgcaccat caaaatcagg ccaaagcgct tttgtaaaat acagctcact
    17041 atatgcgatc tgccataaca gaaaattact aattcgctct tctccacttg tacggatcat
    17101 cagctcagga tcttgtaact ctttaggcaa gaaacctgtc attaaatgct tagcaatcaa
    17161 ttcatcatcg atttcttctg cgtgtatttc tccttttgct acttcttcgg caatttcttg
    17221 gacagcagtc acgatctctg ctctacttcc ataattcaaa gcaaaattca ataccattcc
    17281 agtattgttt tttgtttgct caattgcacg tcttacagca tcttgcgtat gttctggcaa
    17341 tacgttttca taacccatga catgaacttt gacattttct ttaatcaatt ctggtacgaa
    17401 cgtatcaaag aagtcgacag gtagttgcat gagaaaactt acttcatctt ttggtcgctt
    17461 ccagttttca gtcgaaaagg cataaagcgt caacactttc actccaagcc ttgatgcttt
    17521 ttttgtgact ttctttactg tttccatgcc ttctttatgg ccagcaactc gtggcaaacg
    17581 acggttttgc gcccaacgac cattgccgtc catgatgatg gcaatgtgct gaggaattgg
    17641 tccatcggta ttaaattgaa attcgctttt ttcttgaata tatttgtttt tttgcggaaa
    17701 aaaacgtaac attctttttc ctcctatact taaaagattc ccactatctt aagtattata
    17761 gcaatatcta actgtttttc ctatgaaatc attgtttttt tatttaaaaa ctgaataatt
    17821 ttttcttttt ttaattttta aataaaagaa atataaataa aaacagagga agcggaatga
    17881 tccgcccctc tgtttttatg attttattaa acttccaata attctttttc tttatcagca
    17941 gcgatcttat ctacttcttt aatgctgtta tctgtcaatg tttgtacgtc tttttctaat
    18001 ccgcgcaaat catcttcggt aatatcacca tttttttgtt gttttttata ttcatcgatc
    18061 gcatcacgac ggatgttacg aatcgcgatt tttgcatttt ctgcttcttt tttcacatct
    18121 ttagctaatt cttttctgcg ttcttctgtc agttgcggga taactaagcg aatgacattt
    18181 ccatcattgg ttggactgat tccgatatca cttgccataa tagctttttc aacatcttga
    18241 agaatgctct tgtcaaaagg tgtgatcatt aaaacacgtg cttctggaat ttggattgat
    18301 gccatttggt tcaatggtgt tggcgcaccg tagtagttca cagtaatacg gtccaaaagg
    18361 ctagcgttcg cacggcctgc gcggatttgc ccaagttcac gttgaagatt ttgtgctgct
    18421 tttttcattt tttcttttgc ttctgtcata attgcatctg ccatttttat ttccccctta
    18481 cagttgttcc gatattttcg cctaagattg cacggcgaat atttccgtgt tcatttaaat
    18541 tgaagacaag cagtggaata tcattatcca tgcttagtga acttgcagtt gaatccatga
    18601 cttgtaatcc tttggcaata acttccagat gagtcagttc ttcaaatttt actgcatttg
    18661 catctacttt cggatcagca gaataaacac cgtctacatt gtttttagcc atcaggataa
    18721 catctgcgcc gatttctgcc gctcgtaatg ctgctgttgt gtctgtcgag aagtacgggt
    18781 tgccggtacc gcctgcaaag ataacgatcc gtcctttttc taaatgacgt tctgctttac
    18841 ggcgaatata tggctctgcg atttgacgca tttcaatcga agtttgcacg cgtgtcggta
    18901 cacccacgtt ttccaacgta tcctgtaaag ctaaggcatt cataactgta gctaacatac
    18961 ccatgtaatc agcttgagca cgttccatgc ccatttgtgc tccgatttgt ccgcgccaga
    19021 tatttccgcc acccacaacg attgccattt caacgcccag ctcgtgaact tctttgattt
    19081 cttggatgat ttctttgatt actggtggtt tgattccaaa tcctgcatcc ccagctaaag
    19141 cttctccact caattttaag actacacgct gatatttcgg tgtgaccatg ttcattcctc
    19201 caatattttc tattgccatt ttaccatact tagatgattt cgacactcta agactggttt
    19261 ttcttgtttt gcttaaaaaa agggagcgca caaaccgtgc gttcccgtct gttattcaac
    19321 agaattattt tttcacttgg ctcataactt cttctacaaa gttatcttcg cgtttttcga
    19381 taccttcgcc aacttcgaaa cgtacgaatg ttttaacagt tgcgcctttt gatgcaacgt
    19441 atttttcaac agtcatatca ggatctttaa cgaatggttg gtccaccaaa gcgatttctg
    19501 ctttgaattt cttcaagcgt ccttctacca ttttttcaac gatattcgct ggtttgcctt
    19561 cgttcaatgc ttgttctgta agaacagttt tttcatgttc taattcagct tctggaattt
    19621 gtgattcgtt tacataacga ggattgatag ctgctacgtg catagctacg tctcttgcaa
    19681 ctgtttcgtc agttgttcca tctagaacag tcaatacggc aatacgtccg cccatatgca
    19741 agtatccacc aaatgcagca ttgtcgtctt tttctacaac ttcaaagcga cggaagctga
    19801 ttttttctcc gataacttga gttgcttcga tcaagtcata ttcaattgta cctttatctg
    19861 ttttgatttt cattgcagct tccatgtctg ctggtttgtt ttcagcaaca agttctgcaa
    19921 tttcttttac taaatcttgg aacatttcgt ttttagaaac aaagtctgtt tctgagttta
    19981 cttcaacgat tgctgcagtg ttacctttca cagcaactga agccaatcct tcagctgcaa
    20041 tgcggtcatt tttcttcgca gcctttgcca ttcctttttc acgtaaaaga tcaactgctt
    20101 tttccatgtc gccttctact tcaactaatg cttttttcgc atccatcatt cctacgccag
    20161 tcatttcgcg tagttctttt accattttag ctgtaacgtc tgccatgttt aactcctcct
    20221 gtgtgttttt ctttttaaaa aaagctgctt caaaggagag tgaggcttgt cgcccagcct
    20281 ttgaaacagc tccatcattc aaaattattc tgctgaagaa ttgtcgcctt caacaacatc
    20341 tacgatttct tcgattgatg ttgctgtgtt ttcttctgtg aaatcttctt caactacttg
    20401 atcttcacct tggtttcctt cgatgaaagc atcagccatt tttgaagtaa tcaatttaac
    20461 ggcacgaatt gcatcgtcgt ttgaagggat tactacgtcg atttcatctg gatcgcagtt
    20521 tgtatcaacc atagctacga ttgggatatt taatttgtgt gcttcttgaa cagcaatacg
    20581 ttctttacgt gggtcaacga tgtacattac atctgggatt cttggcatat cagcgatacc
    20641 gcctaagaat ttttcaagac gttcacgttc tttgttcaaa ccagcaactt cttttttagg
    20701 tagaacttca aaagttccat cttcttccat tgcattgatt tgtttcaaac ggctgatacg
    20761 tttttggata gtatcccagt ttgtcaatgt tccgcctaac caacgatggt taacgaagta
    20821 ttgtcctgca cgagttgctt cttctttgat tgcttcttgt gcttgttttt ttgtacctac
    20881 gaataatgct acgccgcctt cttcagcaac gctcttcatg taatcataag ctgcatctac
    20941 taatttaact gttttttgca agtcaatgat gtagattccg tttctttctg tgaagatata
    21001 tttcttcatt tttggattcc agcgacgagt ttggtgacca aagtgtacgc cggcttctag
    21061 taattgtttc atagaaatta ctgccatgtt tgtttcctcc gatttggttt tatttttcct
    21121 cttcttatct tctaccttgc cggcaaactt tttcaagcac ctaccgacaa tccaataaga
    21181 atgtggattt gctgatgcat aaagcaccct cagctattat acagacaaaa gatccgtatc
    21241 gcaagagcaa atatactttt ctgctcaaca ttcaatagtt tacctgaaag ataaaaagaa
    21301 ttcaagaaaa acagtagatt tctctttgtt tctttattat cctttttcca taaaataatt
    21361 ttaggtataa aagacgaata ttattgggca atccttcttt tttttgttat gatacatgtg
    21421 aaatctttcg taaaggagaa agaaaatgcg caaagctcaa agacatttgc tgattaaaca
    21481 agtcattgaa gaattcccga tccgtacgca agaagaacta ttaaacaaac ttgggcatta
    21541 tggcgttacc gccacgcagg caacgatttc tcgagatatc cgtgatctaa aaatcgtgaa
    21601 ggcacctgat gaaaatggcg tttcccgctt cgtattgttt cgcgaaaaag gaactcctga
    21661 gactaggact gaggaagagg atcggctggt tcaaatgatt gaagacattg tattgaaagt
    21721 cgaacgagtc cactttttga cgatcgtcaa taccttgcca gacaatgctc atttatttgc
    21781 atctgtttta gatgagatca aacctccctc tattgtcagt acaattgctg gattcgatac
    21841 gatcatggtc atctcggaaa atgaggagga cgcaaaacga gtagaagaat ttcttcacca
    21901 tccaactgaa gtatcacttt tttaatcaga gcacgtattc ctaatatgga atgcgtgttt
    21961 ttttattttc aatattttcc ttattaaata aacctttaca ctctttacaa aaatacatca
    22021 ttttttattc aattttttca acaccttgat tgaatcacaa taatcatcat ttatattgga
    22081 tgttaacaag agcagcttct tgctctttgt gtatttcaaa aaagaaatga aaggaaatca
    22141 tatgtataaa ttattaactc ctggaccatt gacgacttct gaaacagtga aaaatgctat
    22201 gctgatcgat cattgtactt gggatcaaga atacaaagaa attacacaat ggataagaaa
    22261 aaaattattg gatcttgctc aagtatcgga aggaatatac acaacggtct taatgcaagg
    22321 tagcggctcc tttggggtag aatcggtatt aagttctgtg atcaggccag aagatactgt
    22381 actgatttgt tcaaacggcg catatggaca acgaatgatc aacatgtgcg aatgtctgaa
    22441 actgaactat accgtttatg aggtatcgta acatcaaatc ctccaagctc atgtgattac
    22501 acagctgatt gaagagaatc cctctatctc cgcactagca atggtccata gcgagactac
    22561 ttccggtatt ttgaataatt tggaagagat tgctgctgtc acacaagaaa aagatctttt
    22621 gtttatagtg gacgcgatgt caagctttgg tgggataccg attcgtgtag ccgaactagg
    22681 gattgatttt ttgatttcca gctcaaacaa atgtattcaa ggtgttccag gcttttcttt
    22741 tgtacttgct aataaagcgg aactagaaaa gcgaaaaaat gtgagtcgaa gtttgtcact
    22801 tgatctatat gaccagtacc gcgtgatgga aaacgagaat ggcaaatggc gtttcacttc
    22861 ccctacccat acggttttgg cttttcaaca agcgctgaag gaactagagg cagaaggagg
    22921 aatcgaaact cgatattcac gttatctcgc caataatcat gtactacgac aaaaaatgac
    22981 agagatgggg ttccagcctt tcattcaaga atatcagggg ccattcatca cctcttttct
    23041 ttaccctaag gactggcgat ttgattttaa tcatttttat cagtatttaa agcaaaacgg
    23101 atttgtgatc tatcccggga aaatcagcga tcatgaatgt tttcgtatcg gaaatattgg
    23161 cgagatctat ccggaagata ttttggaact gacagcaatt atcaaagact ataaggagaa
    23221 cgttaatgat taaaactgta atttttgatt gggcaggaac aactgtggac tttggctgta
    23281 tggctcctgt ccacgctttt agaaatgctt ttctagaaaa aggaatccaa ctgactgaca
    23341 aagaaatccg cgaaccgatg ggaaaattaa aatgggatca tatccaacag cttctttccc
    23401 tcccttctgt caaaaaacaa tggatagcta tttatggaca actacctcag gaaaaagagg
    23461 tggaggaatt gtatcaacaa tttgaacatt atctgtttaa agatttggtc aagcattcta
    23521 ccttgaaacc cgacacattg gaaacgatcg aacggttgag aaacgctggg atcaatattg
    23581 gttccacgac tggatttact gctcctatga tgacaatcgt agcagaaacc gcaaaaaaag
    23641 aagggtattc accagacttc atggcaactt ctgaagacgt cgaagggtat ggtcgaccgt
    23701 ttccttatat gatccataaa aacatgcagt acttcaaaaa caagtcggtt gatgaagtaa
    23761 tcaaagtcgg agatacggtc tcagatatcc aggaagggaa aaatgccgga gtgttgacag
    23821 taggtgtaat tgaaggtagt tcgctaatgg ggatttctta tgatgagtac caaaaattag
    23881 agatggggaa aaaagatcgt ttaaaacagt ctgttgctct tagattccaa caagctggcg
    23941 ctgatatttg tgtaaacaat ttaaaagagt taacagaata cattttgtac gctgacaaga
    24001 atccagaatg acttttatac aaaatcagag gtcgtgaaaa agctgtactg catcaagtaa
    24061 taagaacagc caaacaaaaa tagctcctca tatttttcgt ttggttaggc ttattaccgc
    24121 agatgcaagc ttttgaacac cgtttattcg aaaaaaaaag ggagctgtga cacgactttt
    24181 gtcacagtcc ccttatttac aaacaaacag tgtttaagag acaacctctc cactaaacgg
    24241 tgttcattca gctgactctc ggtatttgct caaaaaacga taaaatttga aaagctattt
    24301 tctcttttgt ttctctaata ctcggatcag aacgctgatt tcacattctc tttttcttac
    24361 atcttatctg gtgcatgaag tcctagcaaa cgaaggtctt ctttcaataa tacagtcact
    24421 gcataaacta gagctaagcg tgcttctttt tgttcatcat cagctaaaat cttcgtatga
    24481 gcatagtatt tgttgaatgc ttgtgctaat ttgattgcat gtttcgcaat cactgacggt
    24541 tcgtattttt ccccagcaga caagaccgtt tcagggtatt tttgaactaa tttaaccact
    24601 tcccagctgg tatcgtcatt caatgcatag tctgctgttt ctgaagggac aaatcctgct
    24661 ttttccaaca ggctgactgc acgagcatgt gtatattgca cataaggtcc agtttctcct
    24721 tcaaatcgaa caacttcttc caggttaaag tcaaatgtat tcagtcgatc gtttttcaaa
    24781 tcgtggaaaa tgactgcccc aaccccaact tgtttagcta cagcatcttt gttttccaaa
    24841 tcagggtttt tctcgctgat ttgttcttta gcagattgga ttgcttcatt taatacttct
    24901 tctaataaga cgatctttcc tttacgagtt gataattttt taccaccttg cgtgatcaaa
    24961 ccaaatggaa tatggcgcat atcgtcagac cagtcaaagc ccatttcctt caagacagct
    25021 ttcaactgtt taaaatgata gctttgttca ttccctacta catatagaga ttgtttgaag
    25081 tcgtaagtac gtttacggta caatgcagca gctaagtcac gtgtaatgta tagtgtcgca
    25141 ccgtctgact tcttaatcaa cgctggattc aggtcataag cagataggtc aacgatctct
    25201 gcacctttat cctcgttcag caaatgtttt tcttctaaaa gtttgacgat ttcatccatc
    25261 ttgtcattgt agaaagcttc accgttcaac gaatcgaaac gtacttccag cagatcatag
    25321 attttattga attctttcat cgattcatca cggaaccatt gccataattg aatagcctct
    25381 tcatcgcctt cttccagacg tttgaaccaa gcacgagctt cttcatttaa ttctggttct
    25441 gtttcagcta cttcatggaa ttggacatat aaacgaagca attcattgat tggttctgct
    25501 ttaacagctt cttctgtacc ccattttttg taagcgacga tcagttttcc aaactgtgtt
    25561 ccccagtcac ctaaatggtt gatgcggatt ggctgataac cgattttttc cataatgaaa
    25621 ccgatcgaat taccgataac tgtcgaacgc aaatgtccca ttgagattgg tttggcaata
    25681 ttaggagaag acatgtcgat tggtactgtt ccttgattcc cgatattgct atccccataa
    25741 tgttcttttt ctttcactac tgtttgcaaa acttttttgc tgataagttc tttattcatg
    25801 aaaaagttca agtaaggtcc gacaacttcg attttttcaa agttggcaga atcgattttt
    25861 tctgctaaat ctgcagcgat ttgctgtggc gcttttcggt aaacttttgc taatgaaaag
    25921 gctggaaatg caacatctcc gtgttcagct gattttggat tttctagtaa ttgttcgact
    25981 tgttccagtg ttaaatcatc tttgacagcg tcatagacag cttttgcgac aagatcttta
    26041 ttattcatct ttattcctcc taaagtgctc ccttgattgt taaacaaaaa aaatctctag
    26101 cgacggcata cttagtatgc catcactaga gacgagattt cccgcggtac cactccaatt
    26161 gtcctaaaaa aggaccagct ccatctgata tcggtagacg accgtctgtt taaaacagaa
    26221 ttctccaagt gcgcttcgtt gtatcttctt cattcggctt ccaccacttc cgaattcgct
    26281 agccaagaat gatacaagta ctctcttgtt caacaaatct tagtcttatt aaactttctt
    26341 gaattatatc agaaaaaaaa acgaaaggct agtcgtttct gataaaaaaa caatccagat
    26401 ggcctaaagg agaagaaata ccacctggat agctataaat catttcgtaa atacgaaaga
    26461 cttaacaaat taatcagcta cgataatcgt tccttcttca gaagctaaaa gattctcgat
    26521 attttctaaa gaagtgatga ttgccttagc gtttggtttt gcttcaacaa atgcaatagc
    26581 tgcctctact ttcggaagca tgcttcctgg agcaaattgc ttttcatcaa tgtattgttt
    26641 catttctgaa acagtaacag tttctaattt cttttgatct ggtttttggt aattgacata
    26701 cacattgtct actcctgtca atacgatcag caaatccgca tcgatgattt cggctaattt
    26761 ttctgaagca aaatctttat cgattacggc ttctcgtcct tctaatccag tagctgtttc
    26821 tacaacagga atcccaccgc caccaacaga gactgtgata actccttgat cgaccagcgt
    26881 ttcgatgaca cgtgcttctt taatcgagat gggtttaggg gaagcgacta cttttctcca
    26941 tccacgtcca gcatcttctt tgaatgtcac agtggagtca gcattcattt gttctttggc
    27001 ttcttcttcc gtgtagaatg gaccgactgg tttacttgga tttttgaatg aaggatcatt
    27061 ttcatccaca ataacttgag tgactaaaga aaccacatct ttatcgatcc ctttttcctt
    27121 cagtacttcg cccatggcat tttgtaacca gtaaccgata gatccttctg tcattgcaac
    27181 acatgtatct aaaggcattg ccggtgtttt ctcagaatca gcagcttgct gctgaatcaa
    27241 taggttaccg acttgcggac catttccgtg ggagatgatc aattcatctc cttgttcgat
    27301 aaatttcact aaatactttg ctgtttccat taacgcttct tgttgggctt ttgcacttgc
    27361 atccgtagac aagattgcat taccccctaa agcgacaact acttttcgtt tggccatttc
    27421 attcgtcctt tcttcagttc taccgaattt tctagataac aaaacgagcc agcgttttgt
    27481 gattttccct caatgctgac tcgttttttc gtgagatgaa tgtggttatg cttaaacgcg
    27541 agggataaac aagttaccta atgttgctgc catgatcgct ttgattgagt gcatacgatt
    27601 ttctgcttgg tcaaattgac gagcatattt gctgcggaag acttcatctg ttacttccat
    27661 ttcagtgatg ccaaagcgtt ctttcatttg ttcaccgtaa acagtgtttg tatcatggaa
    27721 agctggcaag cagtgcaaga agatcaaacg atctgtgttg tgtgtttttt ctaccatttc
    27781 catattgatt tggtaaggtt tcaacaattt gatacgttct tcgaatttat cttcttcacc
    27841 cattgatacc catacatcag aataaaggac gtctgctccg tctacacctt tatcaacatc
    27901 gtcggtaatc atcaattgtg caccggattt ttcagcaaag ccttctgcca ttttgacgat
    27961 ttcttcttca ggttgtaatt cttttggtgc aacgatacgc atattcgctc ctaaaattgc
    28021 acctgtgact aataaagagt tagccatatt gtttcgacca tctccgcagt aagcaacagt
    28081 gataccttct actgttccaa agttttcttg gatagttaaa aagtcagcga tcatttgtgt
    28141 tggatgccat tcatcagtca agccattcca aactggaaca ccagagaatt ctgctagttc
    28201 ctctaccatt ttttgactga atccacggaa ttcgattcca tcaaacatac gacctagtac
    28261 tttagctgta tcttctgtgg attctttttt acctaattgg atatcatttg cacctagata
    28321 ttcaggatgt gcacctagat cgatcgctgc agtagtaaat gcagaacgag tacgtgtaga
    28381 tgttttttca aataaaagcg caatattttt tccttctaga tagtgatgtg ggataccacg
    28441 ttttttcaag tctttcaagt gttctgaaaa atcgatcaaa tattgtaatt cttcttttgt
    28501 aaagtctttt tctgctaata agcttcttcc ttggaatact gattctttca tattaaacat
    28561 tctcctttag attgttttta tttaagatta aagaggaaat cccgacggca gctgacagta
    28621 agaccgccgt caggaaacaa attacttcaa gtcttcacgg acaagtggtt ggctcatgca
    28681 gcgtggacca ccacggcctc gtgacaattc acttgaattg atttctaaca ctttgatgcc
    28741 atagctacga agcaattcgt tcgatacata atttcggtta taagtgacta caacacctgg
    28801 agcgattgcc aatgtgtttg atccatcatt ccattgttct cgaggtgcaa cgatctcatc
    28861 tccattacca gttgggatca gtacaagatc atctaatcca agagcagctt tcatcgtttc
    28921 gtgtaagtta tctgaatgag taatcttgat atcatcacca tctggttcga ttgtaaagac
    28981 atccatctta ccgtctttac tttgaattgc tggatggata gtgaatttat cgtgatctac
    29041 cattgtaaat acggtatcca aatgcatcat tgcacggttg tttggaattt tgattgctaa
    29101 cactttttca aatcctgaat tttttgcgaa taggtggcgt gctaattttt ctaaagcttt
    29161 cgcattagtt cgttgagaaa tcccaacagc tactacttta tcgcttaaga tcaattcgtc
    29221 tccgccttcg atatgatctg gattttcacg atccaaccat acttcgattc ctttatttgc
    29281 aaatcttgga tgatgtttca aaatatattc tgtaaacatc gattcacgac gacgtgcttc
    29341 aaaagtcatc ttattgactg tcatcccatt tcccattgat gcggaagggt cgcgtgtaaa
    29401 gtacaagttt ggcattgggt ccatgtaaaa tggatagtca tcgtctgctg acagatcgta
    29461 tagactgcta gaacgtactt caatatcttt tgtacgaacc ccagccatga ttttatctac
    29521 catttcttgc gtttccattg atagaaggaa ctcatgaaga ccctctctta cagcattcga
    29581 tgcaatatga gattcatcca acattttatt caagaattgt tctttgacat tgcctgcgtc
    29641 aatcgcttct gccgctaatt tttccaagta aagagtctcg acgccttcat tttgcaatgt
    29701 ctttgcaaag ttgtcatgtt cttcttgagc aatcggaaga tatggaatat catcgaacaa
    29761 cagacgatcc ataatatctg gtgtcaaatt ttccacttct tgtccaggcc ttttcagtaa
    29821 aactgtcttt aatttcccta tttcagagaa aacgtgaata ggtttatcca tgttgtgttg
    29881 cctccttctt tttgattaca tgtataggat atcctccttt tgtaatcact ttcataattt
    29941 tgtctcattc aaaatgagac attcctcaca ttaatattcg ataattaaat aattaaacgt
    30001 ttttctattt ttaagcg
//
LOCUS       DAZBTF010000029        29100 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000029 DAZBTF010000000
VERSION     DAZBTF010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.029"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1031)
                     /locus_tag="ACHGNS_001369"
     CDS             complement(<1..1031)
                     /locus_tag="ACHGNS_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN6937324.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSVVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="ACHGNS_001370"
     CDS             complement(1113..1802)
                     /locus_tag="ACHGNS_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFN6937325.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="ACHGNS_001371"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="ACHGNS_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFN6937326.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="ACHGNS_001372"
     CDS             complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="ACHGNS_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFN6937327.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3036..3163)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="ACHGNS_001373"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="ACHGNS_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFN6937328.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="ACHGNS_001374"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="ACHGNS_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFN6937329.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="ACHGNS_001375"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="ACHGNS_001375"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFN6937330.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="ACHGNS_001376"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="ACHGNS_001376"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFN6937331.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7544)
                     /locus_tag="ACHGNS_001377"
                     /pseudo
     CDS             complement(6444..7544)
                     /locus_tag="ACHGNS_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(7636..8523)
                     /locus_tag="ACHGNS_001378"
     CDS             complement(7636..8523)
                     /locus_tag="ACHGNS_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFN6937332.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="ACHGNS_001379"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="ACHGNS_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFN6937333.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="ACHGNS_001380"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="ACHGNS_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFN6937334.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="ACHGNS_001381"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="ACHGNS_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFN6937335.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /gene="cbpA"
                     /locus_tag="ACHGNS_001382"
     CDS             9754..10398
                     /gene="cbpA"
                     /locus_tag="ACHGNS_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFN6937336.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="ACHGNS_001383"
     CDS             complement(10454..10957)
                     /locus_tag="ACHGNS_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFN6937337.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11554..12519
                     /locus_tag="ACHGNS_001384"
     CDS             11554..12519
                     /locus_tag="ACHGNS_001384"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFN6937338.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12618..13790)
                     /locus_tag="ACHGNS_001385"
     CDS             complement(12618..13790)
                     /locus_tag="ACHGNS_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6937339.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13777..14922)
                     /locus_tag="ACHGNS_001386"
     CDS             complement(13777..14922)
                     /locus_tag="ACHGNS_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6937340.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14936..15868)
                     /locus_tag="ACHGNS_001387"
     CDS             complement(14936..15868)
                     /locus_tag="ACHGNS_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6937341.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15985..17064
                     /locus_tag="ACHGNS_001388"
     CDS             15985..17064
                     /locus_tag="ACHGNS_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFN6937342.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17061..17679
                     /locus_tag="ACHGNS_001389"
                     /pseudo
     CDS             17061..17679
                     /locus_tag="ACHGNS_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(17789..18016)
                     /locus_tag="ACHGNS_001390"
     CDS             complement(17789..18016)
                     /locus_tag="ACHGNS_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN6937343.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18119..20707)
                     /locus_tag="ACHGNS_001391"
     CDS             complement(18119..20707)
                     /locus_tag="ACHGNS_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFN6937344.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20727..21386)
                     /locus_tag="ACHGNS_001392"
     CDS             complement(20727..21386)
                     /locus_tag="ACHGNS_001392"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFN6937345.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLLNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="ACHGNS_001393"
     CDS             complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="ACHGNS_001393"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFN6937346.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22159..23025)
                     /locus_tag="ACHGNS_001394"
     CDS             complement(22159..23025)
                     /locus_tag="ACHGNS_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFN6937347.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23134..23775
                     /locus_tag="ACHGNS_001395"
     CDS             23134..23775
                     /locus_tag="ACHGNS_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFN6937348.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="ACHGNS_001396"
     CDS             complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="ACHGNS_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFN6937349.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25216..26115)
                     /locus_tag="ACHGNS_001397"
     CDS             complement(25216..26115)
                     /locus_tag="ACHGNS_001397"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFN6937350.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26117..26914)
                     /locus_tag="ACHGNS_001398"
     CDS             complement(26117..26914)
                     /locus_tag="ACHGNS_001398"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFN6937351.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26892..27596)
                     /locus_tag="ACHGNS_001399"
     CDS             complement(26892..27596)
                     /locus_tag="ACHGNS_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFN6937352.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27727..28302
                     /locus_tag="ACHGNS_001400"
     CDS             27727..28302
                     /locus_tag="ACHGNS_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFN6937353.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28420..29097
                     /locus_tag="ACHGNS_001401"
     CDS             28420..29097
                     /locus_tag="ACHGNS_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFN6937354.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8564 a         6167 c         5230 g         9139 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactac acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctag ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    11401 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt
    11461 cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa
    11521 atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc
    11581 aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta
    11641 aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag
    11701 attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca
    11761 atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt
    11821 gatactttct atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga
    11881 aatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt
    11941 ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa
    12001 aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg
    12061 acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat
    12121 ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca
    12181 ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt
    12241 tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt
    12301 gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag
    12361 aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt
    12421 tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat
    12481 atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag
    12541 ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc
    12601 ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct
    12661 ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca
    12721 ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta
    12781 ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc
    12841 aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct
    12901 ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta
    12961 atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac
    13021 gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag
    13081 taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc
    13141 tggtttcgtt tactgatctc tttgcgatta aacgctagat tgatgacgct gaaaccgcta
    13201 aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt
    13261 ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt
    13321 tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt
    13381 tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc
    13441 tgtatcttga tcgtcgccat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga
    13501 tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt
    13561 ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca
    13621 ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca
    13681 tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa
    13741 ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc
    13801 tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag
    13861 attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac tttcgctgat
    13921 caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc aatacttcac
    13981 ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat
    14041 gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg tacctaacaa
    14101 aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac gttcaccaaa
    14161 gtctacttga tagataaaac gaaagacagc taacataata taaatctctg tcaaaacaat
    14221 cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag gaatcaagga
    14281 caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc ggacccccca
    14341 catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac ttttgaaact
    14401 catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa cagtcatgat
    14461 gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac caatacgctg
    14521 taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt gacccacatg
    14581 gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg catttagctt
    14641 ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat cattgacttc
    14701 gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg ctttgattac
    14761 aattccagta ttaatcgttt caagattgcc accttgatca atctcgccaa aggccgccat
    14821 aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg atttatttcg
    14881 cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc ctcctctagt
    14941 ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct tctttggttg
    15001 ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc aacaatactg
    15061 tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt ccatcagaag
    15121 ctattgttcg ctgaatcgct gacaacacat tttctgggag taatgggatg tcgatcgtca
    15181 aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct
    15241 gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg
    15301 ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat
    15361 tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt
    15421 gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac
    15481 gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc
    15541 ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta
    15601 gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat
    15661 tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag
    15721 aaagcataca gttgatggca gtcgatttcc cactgccgtt tggacctaac agacctaaga
    15781 cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt
    15841 tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat
    15901 ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca
    15961 tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt
    16021 ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc
    16081 tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat
    16141 tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt
    16201 ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg
    16261 ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc
    16321 gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt
    16381 gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa
    16441 caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa
    16501 cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt
    16561 ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa
    16621 aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc
    16681 catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct
    16741 tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc
    16801 ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt
    16861 gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcacc
    16921 agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa
    16981 gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt
    17041 ttaccaaggg agggacacca atgataacag tagtcattat tgatgatgat ccatttgtga
    17101 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    17161 attgtgccaa ggatgcgcga aacccatcgt cccgatgttt tgctgacgga catccgcatg
    17221 cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc caaagccatc
    17281 cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt ttccatcggt
    17341 gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt caaatccgct
    17401 tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca aatgatgaag
    17461 aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga gcttgcaatc
    17521 atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt gtacttgagt
    17581 gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt acgggatcgc
    17641 acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa cacaaaaaaa
    17701 cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt caattgtccc
    17761 gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca ggattcgttg
    17821 gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt tctagttttg
    17881 attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat tctttttcca
    17941 tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct aatttatctt
    18001 cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca tatcacactg
    18061 aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat ctttaaaatc
    18121 aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca atcatcggat
    18181 ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa acagcaggtt
    18241 ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt tccactgttt
    18301 ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt agcttttctt
    18361 ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt ccgaccaaag
    18421 aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca ttatccatga
    18481 tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa gtgacatagg
    18541 cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc gtgtagagca
    18601 aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc atcttgaatt
    18661 cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta ttccattcgt
    18721 ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc actttatctt
    18781 cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg aatacattca
    18841 attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg gtgtcgttcc
    18901 aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc attttattta
    18961 acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata tcttgtgcac
    19021 ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca atttgataag
    19081 catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt ggcagacgtc
    19141 cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc cgataaatct
    19201 gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt acttgaccag
    19261 gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata ttcgtcggaa
    19321 tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca tcaacaatca
    19381 aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt ccattcagcc
    19441 ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt cgtttagctg
    19501 ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat tttgaaggat
    19561 ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc acagtagttt
    19621 tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct tcagcgatag
    19681 cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc cgaatattct
    19741 tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg cgctggatcg
    19801 aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta gtctcttctt
    19861 gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga tcaggctgga
    19921 tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat gtctccgccg
    19981 gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag atagctgctc
    20041 gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta tctgcacgtt
    20101 tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg atgatatcca
    20161 aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg aacccgtaac
    20221 gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc tcagcaagca
    20281 tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga tcagcaatga
    20341 ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt ttcttcccga
    20401 ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt gttggctttt
    20461 cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc actaattctc
    20521 cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg actacaatct
    20581 ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact ttgtaaaaat
    20641 tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc atttcttctg
    20701 gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt ttcagttcct
    20761 aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca aggctcttca
    20821 gtctctaaaa tcgtcaaact actatttaac aatccaccca tttcacgcaa ctcacttagc
    20881 tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc tacgaataaa
    20941 agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat acgtgtgatt
    21001 gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa atgattacgc
    21061 aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg accttctaaa
    21121 gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg acgagcaagc
    21181 tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga ataaattgct
    21241 ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta actttgtggc
    21301 aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca ttgtgtcttc
    21361 ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt ttattttact
    21421 ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa ttctaaatta
    21481 gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt cgataaattc
    21541 aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc gttttcacgc
    21601 ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg ctcttctcca
    21661 tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa atgtagttta
    21721 cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat atttacatca
    21781 ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa acctagtggc
    21841 tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt tccagtgttt
    21901 gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc tctcatattt
    21961 atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt atcaactcgg
    22021 tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt tatcagaaca
    22081 ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc ctcaactttt
    22141 ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag ttccttttaa
    22201 taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg ctttgattcg
    22261 ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat tttcctctgg
    22321 tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt cagcaaactt
    22381 ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat aaaaagcctg
    22441 acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc catcttctga
    22501 gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa ccgcgttgaa
    22561 tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg cttcggcaga
    22621 ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag cattttgaat
    22681 ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa catctaatag
    22741 cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat acatgccaga
    22801 ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt taaccgcatg
    22861 ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat ttttgcatat
    22921 caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga acagctgatg
    22981 attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc cccctcattt
    23041 ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg atacaatgcc
    23101 tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg cgctgaaaac
    23161 tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt gtgtgacaac
    23221 ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt aagttatgct
    23281 ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga cttcgtctat
    23341 aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa attagggacg
    23401 gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga ggccttagaa
    23461 ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt tgacagtatt
    23521 cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt tgaccgtatc
    23581 ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg gcttaaagaa
    23641 ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt cactgcagag
    23701 aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac caaaatcgtg
    23761 gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa tgcttattgc
    23821 aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata ttgaaataaa
    23881 tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct actgctggat
    23941 caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc gccactgtga
    24001 ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt ttccaaaaac
    24061 ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg gtcaagactt
    24121 cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca agtcgacgaa
    24181 cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc agcgaaatga
    24241 agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt aaagaagcgg
    24301 tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac gctgccttta
    24361 ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa tcgctagcag
    24421 cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac aaatgatcat
    24481 cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt gccacatctt
    24541 cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca gaaatcattg
    24601 cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc tgaccttctt
    24661 gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc ttcagcacgt
    24721 acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt gtcatgatcg
    24781 cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca tcgtctgccg
    24841 acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc aagtcaacag
    24901 cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc atttcatcta
    24961 ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc gctaattctt
    25021 taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat tggtagaatt
    25081 gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga atattatttt
    25141 cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt tcatccaaac
    25201 caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt ctgcggctaa
    25261 aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca gatcataaca
    25321 atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat catcaccgag
    25381 cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc ctgtatgcag
    25441 ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact cagtctgtgc
    25501 cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc gggcgatcgg
    25561 caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta aggcctgata
    25621 ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg caaatccatg
    25681 ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca catggattac
    25741 ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca ttgtcccatt
    25801 tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta ccagaacgtg
    25861 ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt catgttctcc
    25921 ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca agacattttc
    25981 tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta acagaccctt
    26041 tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt tctcataatc
    26101 ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga tgccaaaaat
    26161 gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa taaattgagc
    26221 ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct tctgtatttt
    26281 ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc aaagtacgaa
    26341 acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata ctaggatgga
    26401 tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta gaaatcgtca
    26461 atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg gctaccatcg
    26521 tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg tccggtgttt
    26581 catccaaata actgatccga acatccagca agggataact aacactttgt tcccggttcg
    26641 ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg taaaacccta
    26701 aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg tagtgatgga
    26761 acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc tcatgatcat
    26821 ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact tctatagtct
    26881 ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct ttcgctttct
    26941 ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg ggagaataac
    27001 cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa ctgataggca
    27061 gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc ttgatattta
    27121 taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg aatctggatt
    27181 tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat caccagatga
    27241 taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg gagaactttc
    27301 atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac aaactgacac
    27361 atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat tggaatatgc
    27421 cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt gacaaattca
    27481 atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag tttcactttc
    27541 aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg ttccatagct
    27601 gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa gttttttcgt
    27661 actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca aaaaggagtc
    27721 gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa gcaagagtac
    27781 gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca gactaatatt
    27841 tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg gatccgtttc
    27901 gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg tctcgaaaca
    27961 acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg tctgcctcgt
    28021 tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc attaatccaa
    28081 accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact tttagctatt
    28141 gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt aggggatgca
    28201 tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc ttatcgtcct
    28261 gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt cattttcaca
    28321 aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg aaagaatcta
    28381 gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat ctatttattt
    28441 gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca atttatcgaa
    28501 gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc tttggtcaat
    28561 ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa tcttgaggaa
    28621 cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc ggcgcagttt
    28681 caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt cggctgtaat
    28741 gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg cggcgatttg
    28801 aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt ttctgatcaa
    28861 aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta caattatgcc
    28921 tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct ttggagaatc
    28981 acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga acatttacat
    29041 acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc taattaaaaa
//
LOCUS       DAZBTF010000030        27866 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000030 DAZBTF010000000
VERSION     DAZBTF010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.030"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(412..1356)
                     /locus_tag="ACHGNS_001402"
     CDS             complement(412..1356)
                     /locus_tag="ACHGNS_001402"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFN6937355.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1652..2596)
                     /locus_tag="ACHGNS_001403"
     CDS             complement(1652..2596)
                     /locus_tag="ACHGNS_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFN6937356.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLKIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2817..4031
                     /locus_tag="ACHGNS_001404"
     CDS             2817..4031
                     /locus_tag="ACHGNS_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN6937357.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            4053..6068
                     /locus_tag="ACHGNS_001405"
     CDS             4053..6068
                     /locus_tag="ACHGNS_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFN6937358.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6151..7524)
                     /locus_tag="ACHGNS_001406"
     CDS             complement(6151..7524)
                     /locus_tag="ACHGNS_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFN6937359.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7550..7834)
                     /locus_tag="ACHGNS_001407"
     CDS             complement(7550..7834)
                     /locus_tag="ACHGNS_001407"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN6937360.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7836..9851)
                     /locus_tag="ACHGNS_001408"
     CDS             complement(7836..9851)
                     /locus_tag="ACHGNS_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFN6937361.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(10030..10335)
                     /locus_tag="ACHGNS_001409"
     CDS             complement(10030..10335)
                     /locus_tag="ACHGNS_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFN6937362.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10424..11455)
                     /locus_tag="ACHGNS_001410"
     CDS             complement(10424..11455)
                     /locus_tag="ACHGNS_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN6937363.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11662..12537
                     /locus_tag="ACHGNS_001411"
     CDS             11662..12537
                     /locus_tag="ACHGNS_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFN6937364.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12689..13531)
                     /locus_tag="ACHGNS_001412"
     CDS             complement(12689..13531)
                     /locus_tag="ACHGNS_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFN6937365.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13623..15500)
                     /locus_tag="ACHGNS_001413"
     CDS             complement(13623..15500)
                     /locus_tag="ACHGNS_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFN6937366.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15497..16918)
                     /locus_tag="ACHGNS_001414"
     CDS             complement(15497..16918)
                     /locus_tag="ACHGNS_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFN6937367.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16921..20310)
                     /locus_tag="ACHGNS_001415"
     CDS             complement(16921..20310)
                     /locus_tag="ACHGNS_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFN6937368.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20568..21959
                     /locus_tag="ACHGNS_001416"
     CDS             20568..21959
                     /locus_tag="ACHGNS_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6937369.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(22032..22679)
                     /locus_tag="ACHGNS_001417"
     CDS             complement(22032..22679)
                     /locus_tag="ACHGNS_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN6937370.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22687..23373)
                     /locus_tag="ACHGNS_001418"
     CDS             complement(22687..23373)
                     /locus_tag="ACHGNS_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6937371.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23379..24452)
                     /locus_tag="ACHGNS_001419"
     CDS             complement(23379..24452)
                     /locus_tag="ACHGNS_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6937372.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24674..25348
                     /locus_tag="ACHGNS_001420"
     CDS             24674..25348
                     /locus_tag="ACHGNS_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN6937373.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25353..26693
                     /locus_tag="ACHGNS_001421"
     CDS             25353..26693
                     /locus_tag="ACHGNS_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFN6937374.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26842..27828)
                     /locus_tag="ACHGNS_001422"
     CDS             complement(26842..27828)
                     /locus_tag="ACHGNS_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFN6937375.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8125 a         5267 c         5049 g         9425 t
ORIGIN      
        1 cctctctcga acggtttttt atcaaaaatc gtcatttttc catcaaaatc tgcttatgca
       61 aaattataaa aaaattttgc agtttttctt actttttttg tcgtttaatt gaatgataaa
      121 aaccagtttc tttattcagt taatcaaata tgaacgaatt agatatgaat cattgtattt
      181 gccgttgcgg aaggattttt taattaacca cagataaaca ttgataacga ttcgcttatt
      241 gtaatcaaca gattaaattt catatgttca tatgtacgga tacattttcg gaccgattaa
      301 ttttaaccat tctatttatt tgcttaccta ttagatatca ataaaaaact gcttaacagc
      361 tatttcagct gttaagcagt tttaggattt ttagttcata ttcattgttt cttataatac
      421 agtgttcaat acttctctta aagcacttgt tgatttgctg aacattgctt tttcttcttc
      481 gttgattgat aattcaacaa cttcacgaac accattttcg tcaacgattg atggcacacc
      541 tgtaaagata tctttttcgt cgtattcacc agttaaataa gcagatacag gcaatacagc
      601 ttgttcgtta ttcaagatag cttttacgat acgtgcagta ctcataccaa taccatagta
      661 agttgctttt ttacgatcaa tgatctcata agcagcatta cggactttat cagcaatcac
      721 atctaattca tcttgggcaa tacgatggtc tttttctaca atttcaaaca ctggtttacc
      781 accgacagtt gtatgtgacc atgcagcaac ttctgaatca ccatgttcac ctaagatata
      841 gccgtggacg ctgcgtgggt caacttttaa tttcaatgcg atttctttac ggaaacgagt
      901 tgtatccaat gttgttccag taccgataac acgtgatgtt ggcagaccag attcgttcca
      961 agcgatatat gtcaaaatat ctactgggtt tgaagcaaca acgataatac cgtcgaatcc
     1021 tgatcccatg atttctttta cgatttggcg catgattgac gcatttgttt taactaaatc
     1081 taaacgagat tgaccaggtt tttggttgat accagctgtg ataacaacga tatcagcatc
     1141 tttacattct tcatagccac ctgaccatac agccacattt tcatcgcccc aagccatacc
     1201 atctaaaagg tccaatgctt ccccttctgc tttttcttga ttcacatcga tcaatactaa
     1261 ttcattagag attccttggt tgatcattgc ataagcgata cttgttccaa caaaacctgt
     1321 accgacgatt actactttac gacttgtttt tttcatgaat aaacagctcc ttttcataac
     1381 tatttgttac tttcatgaaa agtatagtct tgtttattca ttttatcaac atgttaatta
     1441 taatagttgt tattataatt tttgatggaa acgaattcct gaatacgcat aacagataaa
     1501 tgacaaatgt ttacacctat atcccctgat aatacaacca aaagtttaga actgaaaata
     1561 aatccaaaaa aattcgctgc aacgatataa taagacagca atcgcaccta aaacaatttt
     1621 ccttcatatg ctttagtgaa agattttctt cttaatgttt atttctccat tcattgggag
     1681 tacatgttga atacttcttg aataatttga ataaagaagt cgtttgttta tatccgatac
     1741 gattagcgat ttcttccaac gaaaaactcg tttcttgaat cagttccaac gcaaagtcga
     1801 attttttctt tagaaccagt tcttgaaagg aagcatcaaa atacattttc aatttattac
     1861 taagatagac cggattgtag ccaaaatgag ctgctgtttc ttttaacgta caagaagtaa
     1921 aatgcttatc gatataatct aatactctaa gcatctcatt ttctgcgcat ccatctgctg
     1981 atgaaaagct ttgttgttct aataaatgag tcaattcgat cagtaatagt gatgtataga
     2041 gatttaacga acggatctta tgactgtatg tatcaaaata aatcaacagc atattttcaa
     2101 tcaaatgcgt caaacgactt gtttttccgc cgcgaaaatg aatgaattgt gtatgttccc
     2161 ctttttcttt tgatgcattt atcaaaaaag aagaaagtag attatttgtg cttgccaaat
     2221 gattcaatat ctctgtagat aaggtttctt cttttaataa gatgttgatt aaaatatcct
     2281 cttgccctgt gtattcgatt ctttgagtag cttctttatc aatgatcacc acgtcacttt
     2341 cttccatctc tatcctttct ccattaataa tttttaaaca tgtaccgcta tacatataat
     2401 tgatttcaac aaaatcatgt gtatgctcgg gaacttccgc atatcgacgg tgtttactga
     2461 tatgaatatt tccctgttga aatagatccc ctcggtggat ttttggcggt gtctgaagat
     2521 ggtaagctaa gtttaaatca ttgatattca ttccttgttc acgctgcaga gtttcgattg
     2581 attcttcgtt acgcaaaaaa aatgtctaat tcagctagtt ccaattgact ccctcctttt
     2641 tgtaagcggt ttctttcgct tattagttca cgctgtactg ctattatatg caaagctatt
     2701 tttggtttca taaagacttt ctatggttgg gacatgaact tcctgtgatt ccgctattga
     2761 gagaataacc acttcatact atatttttga taagcgctta caaaaatgaa aggaacatgc
     2821 ttatgaataa aaagatgatg aaattttctc tgctgtcagt ctctcttatt ttgacttctg
     2881 ctggtgcagt cacgtctgca attcccagta tgtcagctac ttatgccacg cgttctttat
     2941 ctgctgttga attattaacg actgctccct cttttatggt cactattttt gttttgatta
     3001 gtagttttat tgctaggaaa atcggacaaa aacaaactgt gatcatcgga ttagtgattg
     3061 catcgatttg tggtatcctc cccctcttct cacaaagtta cagtattgtc ttagcttcaa
     3121 gagctggact aggtattggc tttggattga tcaattcttt ggcagtcagc atgattgctg
     3181 ccttttatca cggagatgaa cgagcctcta tgattggttt tcaaagtgca tttcaaggat
     3241 ttggtgcagc tttaatgaca tttgttgcag gacagctagt acagttcggt tggcaatata
     3301 cctttttagt ctacgccatc agcttaccta ttcttgtctt gtttgttttg tttgttccta
     3361 cacctgaaac aactgaactt caatcgcatg gcacagcccc aacaagacaa gtaatcaaaa
     3421 cgaatcctaa aatgattagt tatggtattt ttctcttgct ggtcatcgtg gtatataatg
     3481 ctgtatcgat taaattagcc acagtcctga cgacttattc tctaggaaac gaaactaatg
     3541 cagcgaatat cttaacaatc atgcaattgg caagtatggc tgccggagtt tgttttggct
     3601 ggcttcagac aaaagcacat cgctatatgc ttacactttc tttactactg atgggaatcg
     3661 gctttgtatt gattgccact gtgataaacc tgtatgtgat tgggtttggt gcgatcctaa
     3721 caggaatttc tttttcgtta ttcattcctt acttattcaa ccaagtaagt atccaagcgc
     3781 ccaagtacgc ggagagtttc aatacttcaa tcttattggt tggcgctaat ttaggtactt
     3841 tcattgcacc atatggtttg ctctttctct cttttgcttc tcttgggagt aaaacacttg
     3901 ctgttttcgt caatggcagt atcttgctgt tgattagcgc gcttattagc cttctagtag
     3961 tacacaggac gaatagaaag acagtgataa gagtagatga agaaataaaa gaacaattga
     4021 caacaaaata aatttgatgg aggtcgtgta aaatgacaaa tgaaaaacat aaagatagat
     4081 ggctttggta tcctggagac tttgagatcc gccatggact gctacaaaat ttccaacggg
     4141 aagaacgagg ttttgactgg ccagcgtact ggtacatgga tgattgccat cggaacgtca
     4201 agtttaagag atattacttt cttgatcaac cttccatgtt taaagtgacg atccaaggag
     4261 ttgggtatgt agaaattaat ggacaaaaac atccatgtgg aaaatggctg acttgtccag
     4321 ctggtaaagc aaaaattcgt atctttgtag gacatacatc agggctacca gcgatgttta
     4381 tcgagggaga cgaagtaaaa tcagatattg gctggacagc aagtaatttt attgaagaat
     4441 atccagctgg ctggagtccg ctttatgtcg atatcgcaaa agatccaaat caaatctatt
     4501 atcaaaaaga atgcgtccat ccagtcactg aaacggagat tggcggagga atcttattcg
     4561 attttgggcg agcagtgaat ggctgtgtag aactaatatc tctggatgac catatcgaat
     4621 ctatcactct atgttatgga gagtccgatg tagaagctat ggatatcgat tattgctact
     4681 ataagcaaat aggaatgaca agaaaaatga aagctcgtaa acgcgctttt cgctatttgt
     4741 ttataccaga ggtttctact ggaatggttc aacttcaagt ttttcatgaa tttttatctc
     4801 tttcgaaaca agctgctttt tcaagcgatt cttctttact gaatcagatc tggtcagtat
     4861 cagaagaagc cttcaagtta tgtagtgatt tgttttttat cgacggtatc aaacgagatc
     4921 gctggatttg gtctggagat gcgtatcaat cattcatgat caatcaatat cttttttttg
     4981 acgaggaaat caataaacga acgatattag cattaagagg acaaaatgaa atcaagcagc
     5041 atatgaatac tatcgttgac tattcgatct tgtggttgat tggtatcgaa aaccactata
     5101 tgatgacgaa agactatgaa tttttacgca tcgtctaccc taagatgcaa tcattaatga
     5161 gatacttgat ggagcaaacg aatgaattag ggtttattta tgggagagca aaagattgga
     5221 tatttattga ttggtctgaa atggataaag aaggaacgct tgctgcagaa caagtcttat
     5281 tactgaaagc ctatcattcc atgatcactt gcggagatat cttagctgaa acatatggag
     5341 agaacgaaaa ttatcatact catctctatc aagaaaaata tcaactattg aaacaaaacc
     5401 tatttgacta cttttgggat gaagaacttg gcgcttttat cgattgctat gaatctggga
     5461 gaagaaatgt gacgcgtcat gcgaatattt tcgctgtttt attcgatttt gctaacaaag
     5521 accaacagca atcgatctta aagaatgtct tattgaatga cactatcaca cagattacta
     5581 ctccatattt caaattctat gaacaagatg ctttatgcaa actcggacag acgaatatcg
     5641 tctaccaaac gattcttgat tattggggag ggatgttatc tcatgatgcg gtaactttct
     5701 gggaagaata cgaccctact caaacaggaa atgaaaagta cagtatgtat ggagatcctt
     5761 atggaaaaag cttatgccat gcatggggag caagcccaat ctatctttta ggacgctatt
     5821 ttatcggttt gtatccaaca aagccaggtt atgaagaatt tatgatacaa cctaatctag
     5881 attcatttga aacactgaat tgtacgctac ctatcaaaga tggaaagatt tcgcttgaaa
     5941 aaacaaaaga taagatcgtg attacttctg atagaacagg cggaacagtg aaaatgaatg
     6001 gtaaaagtta tcctcttcta gcaaaacagc cacttgtctt aacaagctat tcaaaaataa
     6061 atgcataaaa caaaaaactg cttgcgacat atcagaagac tttaaatctt cttctgatat
     6121 tgtccaagca atttttattt tgaagtttgc ttaattctgt tctttagaaa caatgtttgt
     6181 tctttttccg gcaaaaagaa agctagataa tacgacaagt aaaaagaaag cgatgattcc
     6241 aactgtcaag gctatttgac cgtattctcc tagtttccca agtacaagtc ctggtactag
     6301 ataatcagta tcggcaaatg tagaagaagc gattttaaga tctcctaata ctggaaggat
     6361 aaataatggc agccaactaa tcaataagct attgaaagca gaacctaata ccgcccctct
     6421 tctaccgcca gtcgagttgc cgaagacacc agaagcagct ccgcagaaga aatgtcctac
     6481 taccccagga atgataactg tagtttgcaa gcctatcata gcaaacatgg atagtgttcc
     6541 tacaaagaag ctaacaaaaa agccaattaa tacagcatta ggtgcatatg gaaaaacgat
     6601 aggaacatct aatgctggtt tagaatcggg tacgagtttt ttagcaatcc cttggaatgc
     6661 tggaacgatt tctgccaaca ccattcgaac cccttgaagg acgataacaa acccagctgt
     6721 gaatgttcca gcttggatca gtgcaaagat tacagcgttg tctccattgc ttagttctct
     6781 ttcaacaaat ccaggaccag ctaacaaagc aagtgcgaca tatacgatcg acatcaagag
     6841 catgatcgat acagtagaat ctcgcaaaaa gctgaaactt ttaggaatct ttatgtcctc
     6901 tgtagaacgg tctttgtttc caaaaaactt tccgatccat ccactagaag cataaccgat
     6961 atttccggta tgccctaacg caacttgatt gtttccagtg atgttcttca taaacggttg
     7021 acaaagagca ggcgtaatag ttaatagtgt tccttcgaag accccgccta ataagatcat
     7081 ccacaaattt gcagtaaacc ccgcgcttac taaaatgact gctgttagac aagaaacata
     7141 aagcattgct tgacctgtaa gaaaaatgaa tttatatctt gtgaatctag caagtaaaat
     7201 attcacgatc atccctacta gcatgatcaa agcagtaggt gtaccgtatt gttgcaaagc
     7261 aatcgccact actgcttcat tgctaggaac tactccttga gtatgcagag ctgtttggaa
     7321 catcgaagca aaaggggtta gtgaattact gattaaacca gcaccgccga tcagtactag
     7381 gaatccgaca aatgttttga tagttccttg gatcacttct gaaaatttct tcttctgaag
     7441 aacgagacca agcagtgcaa tcaaagaaac gaggatggag ggctgactga cgattgacac
     7501 aaacaaattc aacaatttat ccattgatag ttcctccctt gaatcatttt tattcttttg
     7561 acaagatctt cgttactttt tcttttactt cttcgctgtc aatgatcgat tgcaaaggta
     7621 cgagcttttc atcaggtata gaagttagcg tatctttcaa agaatgctca atgaagaaat
     7681 aatcagccat atcgctagat acacttccaa catccgtatg acttacttca aactcgtcca
     7741 catctgctcc aagatctgca agtgcttgtt tgatgttcat ctctaccata aaacttgttc
     7801 ctaatccact ttgacatact gcaaaaattt tcatatcatt cgtctccttt tcttatgatc
     7861 gctaatattt cttcttttgt tgatgcgtgt aataaatctt gaagattttg tttattagaa
     7921 agtagctttg ttaaatttgc caaagcccga agatgtgcct cattatcgat agcagctaaa
     7981 cagatgaaca aactaatggg atgggctgga ttatcaagca gatccacagg ttgttgaagt
     8041 ttcagcaaag acatccctac ttcgtttacg ccatcttctg gacgagcatg aggtaatgcg
     8101 atgtgatcgc caatatggat aaacgcacca tactgctcca cgcgctcgat catagctttg
     8161 atataacttt cttctatttt attttctgat aatagcggct gtgcggcaaa tgtgattgct
     8221 tcttgccagt tttttggttg atccgataat tggatcattt cttctgttaa taattcagat
     8281 agcataggac gttgatcctc cttcttttct aaaagtttgt tcattttacg atttaatact
     8341 tgatagagct tttcttcgtc tacgccttct tttaattcga tataaggcag tagcgccgac
     8401 aacaattctt gggcagaagg cattgaaaat cctggtatca gccaatctct ttgtacttga
     8461 ttgatcagct gatttttttc caagtcagtc atgattggat gcagaacata gagagacttt
     8521 ttcttatcag taagtgtgag aggtacagta gaaaaaataa tatcgtaaga agtttcgtca
     8581 acctcgtgta agcggtcagc tgaattagtc tctgtaaaaa agatcatcgg gaataattgc
     8641 tgaagttcac tttttaagat caatgaagag ctaatgccac ttggacagac gatcacggct
     8701 cttatttttc tatttctttc ttctgcgtcc gcttttctga tctctccgcc aaatagtata
     8761 gtgaaaaaac caatctcttc ttcagggatg ctcttcccta ttcgtctttc taacggaagt
     8821 aatgcttttc ttgtcatttc gtagatggaa gcatattgat gcttaatttg atcgatcatc
     8881 acattaggta aataaaaaga ataggaaata cgaaaatagg cagggaccaa gtgataaaag
     8941 acattcatca ataattcacg aaacgaagca aattctacgg ccatcaggaa ttccatgcga
     9001 tggatgactt ctgaagaaca ttccagcaaa aaatcaagtg caggctcctg gacatctcct
     9061 tcaacgatcg atgctaagca aagagagaaa aatataatct ccgaattttg atgttcggaa
     9121 tagccaaggc cagtcaatac ttgtcgacta atgtcagtga ctgcaagaga agccaacagt
     9181 tcagcatctt ttaccagttc catggatgtt tttccaattc gaatggttgt caaactcaaa
     9241 taataagcta attcatccat tcgatttggt gcgaatcgaa gatctgatag tgaaatcgtt
     9301 ttatctagca aatctctaac tttggcgtaa taatcaatgg aaagggttgc ggttaaatac
     9361 gtgagcccaa atcttccagc aagtgactgc atcaaacgag cagtgcgatt ttttgcttct
     9421 cttcttaata ttgcttctga ccctagcagc ctaaaaccag ttttcctagt atacgacagc
     9481 tgacaatctt ccacttctaa ttcatttctt atctttttga tatctgacag gactgtccct
     9541 ttagaaacct gaagaaaatc ttgaaaatga aaaacagaaa cagacgttgc agtcgaataa
     9601 accatcaaat aaagaagatc tcttcgctct tgttcggtat acacagcttc aaatcgagta
     9661 atatccaaaa atttttctgc ccagtcgatt gctcggaatt tctttatacg gatacgggta
     9721 tcatccacat attcggcaaa aggagttaat tgtttgatcg cctcgcttgt atttacttcg
     9781 atgatctctg ctgctttttc tagactgatt tctcttggtg ttttcaggag ataactaatt
     9841 ttgtagttca cagcgctgtt cacctcctac ttatattcta gtatggtagc gcttttaaat
     9901 cgaggggtgt ttggtacaac ttacagttgg atttttttta cgactgtacc gaacgatttc
     9961 tatcgaatgg atacagaaaa aaagcaggtg accaaatgtc tcctgctttt atgaattgat
    10021 ttcgtccgat caatgatgat catgtttatt ttttgaggtc agttcttcaa atttttcttt
    10081 gacatcttca accgcttttt cgatatcttt ttcaaagtga ttgggctcat taggctttgt
    10141 ttcctcttct acagaatttg aaccagataa agctgtttct ttattttctg tttcaccaga
    10201 gtttgtgcca gctaatgatt cttctttgtc ttctgtttca ccagaattag ttcctgctaa
    10261 agccacttct tcttctgttt taggggccat tgaacctctt aatcgtttgt gtttttcacc
    10321 gtcataattt gtcatgttgg ttcctcctag tagttctata ctttttcatc gtacaataag
    10381 acagtttatg cgtcaaaaaa caaacccgat tcttacaaat atgtcatatt tttccttctg
    10441 attcttgaat gcttttgatt ccttcttcca aaattttcat ttgtaagatt gtttcttcat
    10501 ctttgatgac tttttctgcg ccattaacta atgtctcgta aatcccttcg tagacacggc
    10561 tgtaatcacc aacctcggaa attacttttt cttcatgata actcccttgt tcatctatat
    10621 aggtcaatac accataatgt tcgggcaaat cgataccaaa gtcttcatga tcaggcatgt
    10681 aaaacatttt cagatgttct tcttgtcgat cttttgtttg tttcacgaac atcccttttt
    10741 ttccgtaaac gacgaaactt ggacgttctt tgatgcggaa ataactggat tttatcgaaa
    10801 ttttcatatt tccatagaaa aaatccagat cgaagtaatc attcattcgt cctttcccta
    10861 aaagttgacg tacatcatag ctgatatcat ctggttcgcc gaaataagaa agaacttgat
    10921 ctactgtatg acaagcatgt ccatataaat agctgttcgc agtagaaaat tcatgcgtat
    10981 tttctggtac ttccggtctg aaataatcat aatgcatttc tacttctaaa atatccccta
    11041 attttccgct ttcgattact ttttggactg tcaagaagtc agaatcgaac cgtctgtttt
    11101 ggtaacattg gataaataga cctttttctt ttgctaatgc aaataactct tctgcttcct
    11161 ttgacgtttc tgcaaaaggt ttctctacta gtacattctt accatgttcg agtgccattt
    11221 ttccatattc ataatgaaat tgactaggtg tagtgatgac aactaattgg atctcaggat
    11281 catcccaaag atcagacagc tgatcagtat attcgatgtc ttcgaatgtt ttccacactg
    11341 gttcagatgg ggaaaaaatt tttttgatat tgattttgtt cgtaagtttt cttgaaaaag
    11401 gcaaatgata gcgatttgta ctctttccat tgccaagata agcgattgta agcatgagtg
    11461 gtgcctcctt ttattgatgt tttcagtata tcctagaaaa gcgcagaaag tatttataat
    11521 agctattctg gtaaaaaagt tcagaaaaac acttgttatc attcggtttg gtgaaattaa
    11581 tgttcaaagt aaggataaaa atcaaaaaga tagtagcgga accaagtgtt tatcagtaag
    11641 atagaatttg gaggtcagat catgcttatt gaagaaaaac taacaaaaca agagttgttt
    11701 accacgacag agaaacggat tgtcgattac atccgtagga atatcgaagc tgcagtctac
    11761 atgacgattg aagaattagc aaaagctacg tatacttccc attcagctat cattcgttta
    11821 tgcaaaaaaa tgggattcag cggatttaaa gaattccgtt tcgagctggg gcgggaagtt
    11881 catcaattga ttgctcagtt caatcaaacg attgatccta attttccttt ttcaagtgaa
    11941 gatagcactc agcaaattgc aaagaaaatg gcggatttat cgatccagtc aataaaaaaa
    12001 gcacaattgc agattgaaca tgaagattta gatcaaatcg gtaaagtatt gaacaaggca
    12061 cagcgaatct ttctttttgc aaaaggtgat tctcaaatca cggcaaggaa attccagaat
    12121 aaaatggtca agttgaataa gtttttgatc atggctgaag aatacagtga ttcttcttgg
    12181 aatgcagcca atttgactag cgaggattgc gccattttta ttagctacag cggtcgcatc
    12241 catcattatg aacgaataat gacctatttg aagcatgtag aggcccctac cttgctgatt
    12301 accggaaatc agcattcaga aatggccaaa caggctgata tgtgtcttgt tatatcgcag
    12361 gaggaatatg attttgctaa agtagcgact ttctcctctc aaattgcctt cgattacgta
    12421 ttaaatacgt tattctcagt gatatattct caaaattttg aagagaatat ccgcaacttg
    12481 aaaaacaaac aagaattgat gcaaaaaggc cttcttaaag aatatcggaa aacttaagaa
    12541 aagatcctca tttagtaata agaaagatag tgtttgatag gctctctttg atatcatcat
    12601 aaaaacaggc tgaagttttc tttttcaaac ctcagcctgt ttgtttacgg aacgattcga
    12661 atagatatat tatagaaatg aggaatagct aacttctgtt tcttattttg aacgattgac
    12721 gaaaaagata tataagaagg aggaaaatca gtatacatgc agctaacaat ccatacagga
    12781 aaaatcgttt gttctcggat actttctgta cttctttttg aacttctttt gcatgataag
    12841 gaacccggtg ccctctgaca agtaagcgat gactgttgac catataaggt gtacaagtta
    12901 ggagtgtcac gaaatcctgc cctttttcga ttctcagatc ttttgtatca gtcggttcta
    12961 ccgtcttgat ctgatcgact tgataagcga gtgtccgtcc attgatttcg atatagaaag
    13021 aatcacctaa agtcagtttc ggtaagtctg tgaacaactt tgcttgaggc agtcctcgat
    13081 ggctggaaat aacggcatgt gtattttctc cccccgtagg ataagacgtt ccttccaata
    13141 atgaggcacc tttttctagc agcacagtat tcgtttcatc aaaaattggc aaatggacat
    13201 tgatctcagg gatcgtcaaa ataccgatcg tatgtttctc aaaataggtt ctatcctttt
    13261 tcgcaggttc ttttttctta gtaaagggat ctgcacctgg atttccacct tcttttgcta
    13321 attgctggtt tttctttgtc atgttttctt ggattttagc catgacttct tcattttctt
    13381 tgaccgcttg ttgctgataa tgagagatga tctgctgatc cagataatta ttcagcgcgt
    13441 cgctaacaaa tggataagct agtatcccta ttcctgagag aagcaagaaa agcatcagca
    13501 gatctagaag tttccttttc tttggtttca tttgtgttct ccttgtgatg actgtttgat
    13561 aataaaagaa aaaggagcat ttgcgtaatc cgctgctcct ttttccaatt cttgcaaaaa
    13621 gactaaatct gactgtgctt gcgtctagca aagtacagtc cagcaatcag tagaaggact
    13681 actcctgcac cgatatacac atagattccc ttaccgcctg ttgaaggaag tgtacctttg
    13741 tgtttatttg gtattttttc aggagaaatc aactgtcctg ctgttacata agattgttca
    13801 tcgatagtaa atgctacacg gtttgttaat ggaacatatt tttctggcgc tttcgtttct
    13861 tccagatagt acgtgccata ttttagacct gtcacatcga ttaaaccgtt acttgtggtt
    13921 gtaaaaaccg ttgctgattc tttcgccgat acccagctga cggcttttgt ggatgggtcg
    13981 atcgataaat atttcgctgt gtcactatct tgatcacgaa cgacgaattc tgctccagca
    14041 agtgtttggt ctgatgtaac gtcaccatct actttaacaa atcgtttgcc cccagtaacg
    14101 acatcgactg acggtggtgt ttgatcattt gtatgcccgt tatcgacatt tgcttggtta
    14161 gaaaatcctt tggttggatc tgctttttcg ttcaaatgca tatagtaaac gaatttcaat
    14221 gtaccgccag gagttaatga aggaatataa ttcggatcaa ctgaaaccgt gaatccgtct
    14281 gtttgctcag tgacagaata atttactggg tcgatttctt tatttccatc atataaggca
    14341 taagcatacg ttcctgaaga atcattatca aatgttaaag cagcgtcatg agtatcgata
    14401 agtttgaatg ttgtgtactt gttttgcgtt ccttctttat cagcgatacc taatgggata
    14461 ttgacagaaa tctcatattg aattttttca ccgattgcga catcttttcc attcaattga
    14521 ggtgttgttt tatcaacttt agacgtatca tttttgatgg tcttttctac tggtgtttgg
    14581 ctatttgcca ggatctcaaa aggcgtgatt gtttgctttt caatcatttc cgcattatca
    14641 ggagctttta cttcttctaa attatatttt ccaacttcta aatgattaac gatcaactgg
    14701 cctttttcaa tagatgcctc ggcaaagtca ttgttgccga tgtcataaga atgaccagta
    14761 atgaaatgtt tggctttggt ttgatcagtt gtccaagtgt acaatccatc tgtgacactt
    14821 tggatgtatt ttttgacgct tggtgttcct tcttctttag aaataataaa ttctgctcca
    14881 tttagtgctt cgttttcggc agtaccgatt tttgtaactt tcaacgttcc atcattaccg
    14941 actgtatttt tagggtagag atggatagta tctagttctt ctgtcccgta tttataagag
    15001 ccatctgctt gtttgatcat ctcatataca gggaaagcta aaaccatgtt tgcggcagct
    15061 gacactccgt cttttggttc ttctttgatc gtgtagactg catctttccc attttgtttt
    15121 ttaggtaaag ataaagtgac atttccatca gcatctgtcg ttcctgaagc aactggtgta
    15181 ccaggagtca aagactggac tgcttgtttt gctgcatcca cggacgctcc tttatctcgt
    15241 tgcgcataaa attcttgagt gacgttataa actgaaaatg aaatatcggc taatccttgg
    15301 tattgatcga attcgctcat ttctttcccg ctgttttgga ttaaaggatc gggtaaatca
    15361 gtcattttct ttttatgaag gatgacttga gctgctgtct cctctgcaaa cacttttttt
    15421 gcgccgaagc tgtttgtgag taatggtaaa ataaggaaaa ggactcctaa cataacgttt
    15481 atttttttat gatttttcat tttttcactc ctgttctttt tttgataaac aaccagattg
    15541 caaagcttgc gaataccata ccaacaacgc ctaaaagtgt atttttcgtt tcgtttgttt
    15601 ttggaagaaa tggacggcta ttttcttttt tattgactac tttcaatata ctgtccgcct
    15661 ctttatcctc agaaagaaaa gttgaaatgg taaacggaat ttctgtttca cttaaaatat
    15721 aaccagaagg tgcttcgatc tcttttaatc gataacttcc tgttttcaac ccggaaattg
    15781 aaaaagcgcc atttttatcg gaaatcaggt gaagcgcatc tgattcgttc tttgtccact
    15841 tgtatccgtt ggctgtttgc tggaggtaat ttcctgcttt gtttttaata aggaatactg
    15901 cgccagctaa tcgtttatct ggtttatttg tctcgatttt gacaaattgc ttactccctg
    15961 tatatacaga gactttcttt gtactgacac caaaaccaaa atccagtgtt ccgttgttgt
    16021 tgatttcctt gttcgcctgt gctgtactgc ttagctgcat ctgataagaa atggtcagtt
    16081 tttttccagc atatttttcc aacgaaggga tcgaaaataa aacatcaaat ccgtgaggtt
    16141 tcttgtgtat gacaaagcct tctgtcagct tttctccatt cgatgaaatc gttaaactat
    16201 taggtaaaaa cgttaatgct tcatccgcac tatctgacaa ttcgaacttt tgatagtcaa
    16261 ggatatttac aggaattgtc gtatgcaagg aataagtgat tttttcacca atcgtaaaat
    16321 catttctagg ctcttctaat accttctcaa aaggtggatc agggtagtcg ttttcttcat
    16381 ttttaggata caaatggata gttgaaagtt tttgattgtt ttgtccatgt acaggaagaa
    16441 caactaccat attctctgcc ttttctttga cgacttctgg tactttggat tcaatgaaaa
    16501 gatacgcttt atctcttttt tcttgatctt tagcggccag agaaaaagaa gcgataccat
    16561 cttcgttgtt aagagttgta gttgttgctt ctgcggtaaa cataccggaa gacgcaccgt
    16621 tttttgcgat ctctgcttgt gcttcttcta ccgttttgcc cttttcccgt agatggtaaa
    16681 aagaatctgt gacatcataa acttggaatg tgacaccatt taatcctcga tacgtttgta
    16741 ataaagcttt ttcttgtccg tcatttggat gatttttcgg cagttgcccg tttgggaaaa
    16801 gcaatttgtg taaagtaatc tgaaccattt ctgtttctgt ttctgttgct acctgagtaa
    16861 aagctggttt aaatagtaac aagaagagac acatactaag ccaaccaagt tttttcatat
    16921 ttagctgccc cctctttctt ttttcaaaaa aagataccaa atagaaaacg cacaaccaat
    16981 catccctact agatagattc ctaaacggcc aattcctccc gtttcaggta atggtacttt
    17041 tgcctgattc gtgatgtcta aagaaatctg gttgtttttg gctcctggtg acagaacaga
    17101 ttcatgatct tgtccatcca cttgaattga cccatcttcg tgtataacta tagtaactgg
    17161 ctcttttaga ccttggtatc cttctggtgt aaaagtttcg gtcagcgtat aagttccagg
    17221 ttttaaattt tcgaatagaa aggtatctgt ttcttgtcca tcttttggcg attctaagtc
    17281 catttctggt ccctgcagtc tgaattttgc tccttttaat gtctgtccgt tgtcttcttt
    17341 tttgtgtacc gttagatcaa acgcttttag tcgattttga tgagtgagcg tccatacatt
    17401 atttgtcatc tcggtatttt ttcccgtgta aatgattttt ccatatttat cgatttggaa
    17461 ttcataattt cccggcgaag tatcatatcc ggcaggacca gctgtttcaa cgactcgata
    17521 tttaccaggt gtgagtgaat caaaactggc aagacctttt tcattcgttg tttggctgtc
    17581 aattggctga taagtaccat ttgcttcttt tttctgtagg gaaaatgctg ctcctttaag
    17641 atttatctct ttgtccgtcc cttgcatcgc atacttacgt actgctacag gaacttcaac
    17701 aaaaggattt tcaatagtca actggatcgt atcatcggaa gtagtgactg tttttccatc
    17761 aatggttacc gtaccatcag aagcgatctt gatttcccaa gtagtctttt tgcttaatcc
    17821 atgcccttct ggagcttttg tttctgtcag cgtataggtc atttcttttt gcaatttgac
    17881 attttctgga agagaatagg ttccgtcgcc atgatctgtt agtaaagtat caatagaatc
    17941 tcctgttaat ttgaaaacag cgccaataag atccttttca cctgtagagg aatttttcgt
    18001 tattttcaag tttaacggtg tgaattgttt agtatttttc catgtcattg catctatttg
    18061 ttgagaatcg tatcctggta caggtaattc tttgattgtt tggtaactga atgcttgacc
    18121 ttgattattg tattgaggta gtgataatat ctcttgataa ctttctccgc tatttgcaga
    18181 taatttgtca atgtctgcac gttcccacgt atttgttgta tctttagctg gtttaatgat
    18241 tcgaatatat ccgtttttcc aagctgcagc atttgtcgta tgttcccgtt gaatctcaaa
    18301 agtaacttga tctggacgat cagctagatt gttgtcaaat tcttcccata actttttgat
    18361 gtgaagactg actccgggag ctttagcaga tggtataccg aattcagcta attcattggt
    18421 atcaatgttt ggctggaaag ttgtcctgcc gttcatttga taccagaaat ttggatggaa
    18481 gtcctcattt tctgtttgga ttctcacttg gtaatggatt tggatttctt ggtcttttcc
    18541 taaatagatc tgattgctct tgatggtatt tccttctatg gaaatagatg gagtgaccgt
    18601 tgtaggactt gtccccacac tcttgactga aagtgtacca ggctgataaa tgaatggttc
    18661 tgcgattgga tcatttattt gtccattgct tacagttgct gagatctgta cagctttttt
    18721 ggctaggtat tcagagatat ctgttgcatg atcagctgat tcatagtaaa gatcgccttt
    18781 ttcatctgat gaaaccattt gtcgcatacg agactctact tctgcttttg agagaccagc
    18841 tgccggatcg ctttgaagtt ggatgccaag accatgtatt tcgattcctc gttctttgag
    18901 tgccatcgct tctccgatgg ttgcgataaa cgtactgtcg attcttctgg atagattgtt
    18961 ttggtcaggt gcatcataga ttcttgagat tagagaagta tttcccggcc gatcttgcgt
    19021 attagaaaac tgagttccgt aataattgct gcttgattgt gcgtgtaccc gctgtacttt
    19081 ataggaaaat gttggtacac catccgtcag caaaacgatc acttttttat gtccatttgg
    19141 aacggatagc atgcttcctg catctcttag tgctttttgt gtaaaagtac caccatttgt
    19201 ccgtgaaggt gtaatggatt ttacttgatt tttcactgaa tcaaatgaac ccatctgtac
    19261 tgcaccgtta ctgtagctat atccttcgct tgagtagccg acatatccca tattgatttt
    19321 gtctgtgata ccgctatctg ctaaagtatc gacaaaacga tcgacaccaa tctttacttc
    19381 accgatccga ttattgtcgt tcatacttcc tgaccagtct acgaccaata cgagatcaag
    19441 aggcgtgata tctttttgta catttccacg agcattcaaa taaacatcaa acaatccggg
    19501 tgtacttgtt tcctttgcat actttcgtat cgcatagtct gcttgattac cggttcctcc
    19561 atattcgata tacgaatggg tccgatcatt aggatctcca ttccaagaat ttataccatc
    19621 ccattgattt gttccgtttt tattgccttg atgatcaaga acatttgtat ttcctgtagg
    19681 ttgccaactg gctttaggat aagtgccctg atcatctgtt gtatattctg taggaatcgt
    19741 ttgattagat ggcacactca cagctggcaa ggataatgta ggcaatgtaa atgggctgac
    19801 agaattttcg ttttcttttg atgattcttc ttctatttcg tctggctttg gtactgacac
    19861 atctgtatct ttcttatttt ctgtctcatc aataatgtca tattctttat tatcgacgat
    19921 cagcttgttt gcctggctgt ggtaaagtgc tggatcgatt ggaaaactca gatcgatagt
    19981 ttgtttagtg tttgatgaga tttccaacgt aattttcccg ctatttacct gataagcata
    20041 gccatctcct tcttttcttt ccacatcact taagtaattt tcgactagac caatttcttt
    20101 ttctatcttt tcagcactgg tgttattcgc atttagcttg atgttcagtg ctttttttcc
    20161 agactgatcc gaaatgactt cagctgaaat tgtttctaaa actgttggat catctgtagt
    20221 gtctgctatt gcagataatg gcacaaagtt tgataaactc aatattatca gcatgaaaat
    20281 aactttcagt ttcttccctg tggtcgtcat ttttcccctc ctttttcttt ttttctgttt
    20341 tgacgtgaat agtgaaaatt attaaaataa acaggcgtta cataattata ttttatttta
    20401 atatgataat tattatacga agggcgctaa agggaatcac accaattttc ataccaattg
    20461 gtttcattta tgaagttgat tccttcgatt aatgagcgtt tcattgaaag aaaagggttg
    20521 agggagaaaa acatatgatc aggttgttgg aaggaaacta tagaaagatg gaaaaaatca
    20581 ttcgaattct gtccatttct gacaagtgga tgactaaaaa agaacttgcc aaacgaattg
    20641 gtagttcaga atctacattt gtaaggtata tcgaagagat caaaatccgc tggggaacgg
    20701 atgtaacgat tcaatcttca caaaaattgg ggtatcgtct tgaacatttg aatgtaccag
    20761 cttatttaaa tgttctgatg gaaatgtcgc aaagttctac aaacagtcgt ttattaaatg
    20821 agattattgt caatccgggt aaaacgattg agtattattg tgaaaaaatt tccatcagcc
    20881 ggtcttcttt tacacgtaag ttgaaacaat gcaatcaaat tttagaaagc tatttattaa
    20941 aagtgatcgt tgaccaaggt tttcagttaa ctagccttaa aagtgagctc cagttgcgta
    21001 tctttgttac ctttttcttt ttaagttact acggaagacg cacgttgcca tttccaatcg
    21061 aaaaaaagaa gatcaaagaa attatggtca agaataattg tcagatgaat ttgtttcagg
    21121 aagaaagccc atatgagcaa tctttcttta ttatgtattt tgttgtgcat ctcatgagag
    21181 aatcccaagg aactccattt gaatggaaag ctggagactc ttatgtacag ttgataaata
    21241 tcgataaaaa agacttctta atcttgaaaa atctcttagg aaaattgaag tacagcagct
    21301 atataaaagt agtgaactat tttgtcaata cgattttttc agaattttcg gagaaagaaa
    21361 aaaatgaaat cagtgagcag atacgaaaaa atatgtccta ctgttcgttt tcatccgctg
    21421 attgcaaaga aaaaaacgaa gaatatgttg ttcaattatt aacgcaaatt tattttttct
    21481 cccgtatttt cccatacgat acgactcatt tgacgatccg tgcaacttac tttgcacagc
    21541 agatgcaaca ccgccagata cgtctatatg ctggatttcg tgcagaactt caattttatt
    21601 ccactctact gaatcagaat ctgatttatt atttatctag ctatatcttt tggatgctta
    21661 tcggtaatcc tgaaattcac cattatactt ctgataaaaa aatactgatt atcagcgact
    21721 taagtcttcg acatagtcaa tatattgaag aatatatcag tgatattctt gctgtgcata
    21781 aaattcactc tgagacaact gctattactg aagatcagct gtcgaaatac aatcttctcg
    21841 agtatgatct gatcgttacg aatcaaccca ttttgaatgc ccatgttcct catatattga
    21901 ttgatgatag tgtgtcattc gcaaatgaag aagaactcat ccgcttattc gagttatgaa
    21961 taatcaaaaa acagctatgt ataaaccacc ctgataaaaa tttcaggaca aatacacata
    22021 gctgtttctt tttattgtat tccttcagca atccaacgag atttatagcg aaagtctttt
    22081 aacaactctt cttttgtcca attatttgca atgagatccg tcaaagtgtt cattacgagg
    22141 gtcatggaaa agtataggaa actaactgct tcttccgacg attgaatgga gaaatgattg
    22201 attcttaata attccagcca tttttcgttc attggagaat cagccgatag ttctctacgt
    22261 ttggttgtct gctgatcatt tcgctgggtt aacaagtata acctttgcca atagatactt
    22321 tcgtgatcta agtcgcagca ccactctaaa tatttttcga tccccaaaaa gaaatgttgt
    22381 ttgttttcgc taatgaattt taagatatct tgatggacta tgtgagaact gcttttgatc
    22441 gtatacaaat aagcatcttc caaatcttga aaatatttat aaaaagcacc gcgcgacatc
    22501 cccatcttgt caacgatttc tgagactttg acttggctga tgtgctgtga gcaaaatatc
    22561 tccagtaata ggtcatcgat tctctgtttc tttgcgttcg ataaattgaa aaaagtttct
    22621 tttggcatcc acatctctcc tttatctctg attatacgtg gatagccctt cattgtcatg
    22681 aaggattcat tccttctttt tctcttcttc tgtcaagtac ccatcttcca ttcgataaac
    22741 gcgatcactc cattgaatca tacggacatc atgagtaacc ataatagtcg ctttttgttt
    22801 ctcatgagct tctttaacta gtaatttgac gacttcatat gcatgatctg tatccaaact
    22861 agctgttggt tcgtctgcta agatcaagct tggctcatta tataaagcgc gagcaattgc
    22921 cactctctgt ctttctccac cagaaaggtc tttaggataa ctatttttaa gtgactggat
    22981 atctaaagat tgcagcaagg aatcgatttt tgttgaatct tctttttttg aagtaacttg
    23041 gtcaactagt tcaaattgtt cctttaccgt taaaaaagga atcaaattag agctttggag
    23101 aataaatcct atttctttaa agcgtaaatc tgctcgtttt ttttctggaa gctcagttag
    23161 tgagtgaccg ttgatcacta tttctccgga agtcggtgtt tgcaatcctc ctgaaatggt
    23221 aaggaaagta cttttacctg aaccagaagg accaatgatg ctgacgaatt ctccttcctt
    23281 tacataaaaa tcaattgctt tcaaagctga gacctttgta tgaccagaac caaatgtttt
    23341 ggtaactttt ttcatcgtta atattttttt catttttatt aacctcctat tgctgtaatt
    23401 gggtctactt tggttatcgt acgtatcgaa aacaatccgc ctagcattgc gacaatgatc
    23461 aaaatcccac tatatagcaa ccattgtgac caaaaaacag caaaaggcat cgcgcttggt
    23521 aaaatcagac tagtcaaata agcaaacaaa agagcaagca gaacgcctac caccccaaca
    23581 ataaaggact gtgctactaa agatttagcg atgaagaaat ttttgatccc ttgtgctttc
    23641 attactccaa aaatagcagt cttctgaagt gtcatcacgt acatgaaaat accgaccacg
    23701 gcagcaacca ctaagaacaa aaagtatatc atcgcattta aggtcatatt ttgggcagaa
    23761 tagccaggaa tactttcaat gaatgtccca atagttaata actgtagacc cttcgatgct
    23821 ttttctttcg aaattttagc tgaatccttt attacgatcc cattgatcgg ctgttcttct
    23881 tcagaagcaa acggctgttg tccatacttc aaagcagtcc acgtatctag ggacgcatag
    23941 atgacgggag aaactgtgta ataagtttca ggaaagatac cgacaatcgt caaaggatcg
    24001 tcgtaactgc caattttgat cgtatctcca attttatagc ctgaatcagc cagattttga
    24061 gagatgatga tttcattctt tgttttaaat tcatgccctt tcgtgagttt tggtaataaa
    24121 aatgcttctt tcgttgtgcc gaaaactgta acattttctt tgttttttcc tttgattgct
    24181 ccgctgtaca atccgattgg tgctttctct cctccttcaa tgtatttcag atctcctctt
    24241 gttaattgtg aagctgcaaa tacttgattt gcatcctccg acaaaacaat ctcttgtgcg
    24301 tcccaatcat cgattgcttt tttaaactct tctgaaagtc cattcgctag tcctgacaac
    24361 atgaatacga catacgcaat caagaacata ataccgacga tcaagccata tcgtaattta
    24421 gaatagcgaa tttctttgat agctagaaac atttgttttt cctccctatg atagattgat
    24481 agtgacaaaa tgtcacttgt tgaatagtgt aactctaaag tgacaatttg tcacttgtgt
    24541 tgtttttttc ttatcaataa attgtctaag acatctttcg ataaaacctt aacatgagtc
    24601 ataaaaaatt tatttaagcc ttatttatgc tttccatgat aaactaaagt gaccaaatca
    24661 atgaggtggg aaaatgatta aactattagt agtagaagac gataaagtat tatccaataa
    24721 tataaaagaa atactcgctg caatcggcga gataacacaa gtttatgatg gtagtgaagg
    24781 actgttcgag ttggaaagtg gagtttatga tcttgcaatc ttggatttga tgatgccgga
    24841 aatgaatgga tatgaagtgt tagcaaaaat acggaaagaa aaaatacaga ctccagtatt
    24901 gattttgact gcaaaagata gtttggaaga taagattgaa ggatttgaac gaggtgcaga
    24961 tgattatcta acaaaacctt tctatcgaga agaactgatc atgcgcgtga aagcattatt
    25021 gaaacgctcg ctaggtctct ttgatgaaaa tgttctgaag tataaggata tcagttgtga
    25081 tttaaaaagc aatgaagtct tgtataaaga acaagttttg cccatacaag gaaaagagtt
    25141 tgaattgctc gtttattttt tacaaaataa gggaatcatt ttgacaaaag agcagatatt
    25201 tgaccggatc tggggatttg attctgatac gacgattaca gttattgaag tttatatgag
    25261 caacttacga aaacatctgc gaagtactgg attggataaa gaaatcaaaa ccttacgtaa
    25321 tgtcggttat atactgcaaa gggtgtgatg caatgaatgg agatattaca cgaaagcaac
    25381 gtagtcgctt ttttgcgaca aatgtagcag catttgcttt tatctttttt cttttgggat
    25441 tcattacttt acagatatta aaccaatctg catacagaga aacagatctg tcattagaga
    25501 aaataaattt tgattctcga acagtacaat tagagataca aagatatcag caaggcgatc
    25561 cctttttaaa cccgactcaa acggaacaaa acagaacaaa catggaacag ccattaatgg
    25621 aaccatcaaa tgaccgtttt aacactcaag tgatcttgtg gtctaaaaat ggtgagatat
    25681 tgaataaagc agctattggt gggcgtttca accaaatcaa agaattaaaa atggatacga
    25741 aaaatctgaa taccatccaa aatattgaat taaatgaaga cagtggctca tctgccctct
    25801 ctttccgttc gattacgaaa aaagcggaga cgaatcaaga tggcgttgcc tacatccaaa
    25861 ttttagaaaa tacaaatcaa atcaagaatt cattagcgac ttttcgcacg attatgattt
    25921 tgtgtatgat tgttttttgg attctctcta ttggtatcag ttattacttg tctagtctaa
    25981 gcatgcgccc tattattctt tcttggagaa gacaaaaaga atttgttgag aatgcttctc
    26041 atgaattgcg cacaccgtta acgattattc aaaatagttt ggaacatctt tttaccaagc
    26101 ctaatcatac tattattgaa gaatccgaaa gcatcgctca agcattgtca gagacgaggc
    26161 gcctcactgg attgacttcg gatttgctga caatcgctcg gaatgattct gatcagcagc
    26221 tgctttcaaa gcaaatgatc aatacacaag aatatatcga acaacttgtt aaaccatttc
    26281 aagagatggc catcatagat ggaaaagagt ttattctcca taacgatgca acattagaag
    26341 taccaattga tgaaaaaaaa atccatcaag ttttggttat tttgctggat aatgcattaa
    26401 aatatacgaa tccagaagat aagatcattt tggattcatc tgttaaccat aaagaatggc
    26461 tgattcacgt aaagaatact ggcccttcta tcagtgaaga agatcaagca aggatttttg
    26521 atcgatttta tcgggaagat caatctcggg cgaaagagac tggcggatac gggcttggct
    26581 tggcaattgc aaaacaggta atcgaacagc atcatggaca gatttctgta tccaattatc
    26641 ttccacaagg agtcgatttt aaagtccaat tgccgttgaa gcctaaaaaa taatgcttat
    26701 ccctaaaaaa gggctgtgac aaaaatcttg tcacagccct tttttgttgc gaataaacgg
    26761 tgttccagaa gtagcttctt cggaaataag ccgaaatttc tttaagctga ccacttctgt
    26821 cacaacctct ttagcttacg cttacgattt tttattgcgg tcttcaatgt gtacatcacc
    26881 gcttgtgtca atatccaatt cttctcggct aacatcttct gtcacttttt caacatcttc
    26941 atgttcttct ttatgaatcc caacttcttc acgaataact gtatgtttgc ttacatctac
    27001 ttgctcttct ttgattggga tagtaatcgt gtcatcttcc aatttttcgt ctgttccctg
    27061 tgaagattga tctgttactg gtttgcgttc gatgacgatt tcttcacgtt tgaccggtac
    27121 ttctaccgtt tcagtatcgt tgaccacatg tttgtggatg tcgacttctc cagttgtcac
    27181 atcatgtgta tttacatcta atttttcttc ttttagttga attgtatcat catctagatt
    27241 tggtttgcga tcgatgtctt cagtcttgct tgtacccatt gtatcagtct cgccaggtac
    27301 gtccatcgga tcagttcctg gtgtcatatt caatggatcc gttgtatttt gaaagcctgt
    27361 cgtgccacct acgtccattg gatctgttcc agtgccagtt tctaatccag tccccattgt
    27421 gcttgctggg ctaacagtat cttgtgtatc cattgtatct tctgttgttt ctggctgacg
    27481 atagcctttt actgtgatga caaattttcc atcagaaata tctcctctat attgatataa
    27541 aggatcattt tctggtgtag ccgtctcaga atcataggta tcaaaagaaa aagtatcttt
    27601 tattttttcc cataaagaac ggtcttcttc accatccatc cgattttcat ctgtttcaac
    27661 atctatacca gacaatccgt caaatcctag tctagatgct gcttcatcag tagtataaat
    27721 aacgatatca tctcgttcat acccttcgtt acgcaaccgt tcaattactg ataatgcttc
    27781 ttccctcgta ctatagcttc cgatgaccgt taaatttttc tcttccatgt aaattcctcc
    27841 tagcaaatag atttatgatt ggtata
//
LOCUS       DAZBTF010000031        27703 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000031 DAZBTF010000000
VERSION     DAZBTF010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.031"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            148..1887
                     /gene="yfcC"
                     /locus_tag="ACHGNS_001423"
     CDS             148..1887
                     /gene="yfcC"
                     /locus_tag="ACHGNS_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFN6937376.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2044..3228
                     /gene="iadA"
                     /locus_tag="ACHGNS_001424"
     CDS             2044..3228
                     /gene="iadA"
                     /locus_tag="ACHGNS_001424"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFN6937377.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3339..3542
                     /locus_tag="ACHGNS_001425"
     CDS             3339..3542
                     /locus_tag="ACHGNS_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFN6937378.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3643..4305)
                     /locus_tag="ACHGNS_001426"
     CDS             complement(3643..4305)
                     /locus_tag="ACHGNS_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6937379.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4318..5820)
                     /locus_tag="ACHGNS_001427"
     CDS             complement(4318..5820)
                     /locus_tag="ACHGNS_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6937380.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6368..8047
                     /locus_tag="ACHGNS_001428"
     CDS             6368..8047
                     /locus_tag="ACHGNS_001428"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFN6937381.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8174..8653
                     /locus_tag="ACHGNS_001429"
     CDS             8174..8653
                     /locus_tag="ACHGNS_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFN6937382.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8872..9552
                     /locus_tag="ACHGNS_001430"
     CDS             8872..9552
                     /locus_tag="ACHGNS_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFN6937383.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9560..10891
                     /locus_tag="ACHGNS_001431"
     CDS             9560..10891
                     /locus_tag="ACHGNS_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFN6937384.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10906..11868
                     /locus_tag="ACHGNS_001432"
     CDS             10906..11868
                     /locus_tag="ACHGNS_001432"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFN6937385.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11979..12317
                     /locus_tag="ACHGNS_001433"
     CDS             11979..12317
                     /locus_tag="ACHGNS_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFN6937386.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12396..13103
                     /locus_tag="ACHGNS_001434"
     CDS             12396..13103
                     /locus_tag="ACHGNS_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFN6937387.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13206..14687)
                     /locus_tag="ACHGNS_001435"
     CDS             complement(13206..14687)
                     /locus_tag="ACHGNS_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6937388.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            14996..15532
                     /locus_tag="ACHGNS_001436"
     CDS             14996..15532
                     /locus_tag="ACHGNS_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN6937389.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            15610..16980
                     /locus_tag="ACHGNS_001437"
     CDS             15610..16980
                     /locus_tag="ACHGNS_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFN6937390.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            17047..17382
                     /locus_tag="ACHGNS_001438"
     CDS             17047..17382
                     /locus_tag="ACHGNS_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937391.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(17463..18320)
                     /locus_tag="ACHGNS_001439"
     CDS             complement(17463..18320)
                     /locus_tag="ACHGNS_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6937392.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            18426..18941
                     /gene="tadA"
                     /locus_tag="ACHGNS_001440"
     CDS             18426..18941
                     /gene="tadA"
                     /locus_tag="ACHGNS_001440"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFN6937393.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            18964..19048
                     /gene="ffs"
                     /locus_tag="ACHGNS_001441"
     ncRNA           18964..19048
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHGNS_001441"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            19258..20643
                     /locus_tag="ACHGNS_001442"
     CDS             19258..20643
                     /locus_tag="ACHGNS_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFN6937394.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSCLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            20680..21357
                     /locus_tag="ACHGNS_001443"
     CDS             20680..21357
                     /locus_tag="ACHGNS_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFN6937395.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            21365..22129
                     /locus_tag="ACHGNS_001444"
     CDS             21365..22129
                     /locus_tag="ACHGNS_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFN6937396.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            22131..22790
                     /locus_tag="ACHGNS_001445"
     CDS             22131..22790
                     /locus_tag="ACHGNS_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFN6937397.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(22820..23329)
                     /locus_tag="ACHGNS_001446"
     CDS             complement(22820..23329)
                     /locus_tag="ACHGNS_001446"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFN6937398.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(23441..24439)
                     /locus_tag="ACHGNS_001447"
     CDS             complement(23441..24439)
                     /locus_tag="ACHGNS_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFN6937399.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            24640..25224
                     /locus_tag="ACHGNS_001448"
     CDS             24640..25224
                     /locus_tag="ACHGNS_001448"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFN6937400.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(25301..26476)
                     /locus_tag="ACHGNS_001449"
     CDS             complement(25301..26476)
                     /locus_tag="ACHGNS_001449"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFN6937401.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            26558..26938
                     /locus_tag="ACHGNS_001450"
     CDS             26558..26938
                     /locus_tag="ACHGNS_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFN6937402.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            27012..27374
                     /locus_tag="ACHGNS_001451"
     CDS             27012..27374
                     /locus_tag="ACHGNS_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937403.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
BASE COUNT         8996 a         4681 c         5779 g         8247 t
ORIGIN      
        1 tcatacatag gagaaaaaaa acaaaataga aaaaagtgat tttctaataa attattcatg
       61 tggaaatgtc atttttactg tataattcat ttgattatcc attttttatt catcaaaatt
      121 actgaaaaaa gaaaggaaga aataaatttg aagaaaacaa attcttcagg aaaaccgaaa
      181 cgcgacttca ctaaattatc gacaccccat acgtatgtca ttatttttgg tgtagtcatc
      241 tttgcatgga tactgacttt tgtcgttcca gcaggaaagt tcagcacgca agatattgaa
      301 tacaaagatg caaatggcga aacaagtact cgtactgttt tacgtcaaga ttccttccgt
      361 tatgcttacg agctggataa gtcgtatgtg tttgatcaat tagaagaact gcaagatcat
      421 ccagcagaac gtgaaaaact ggatgttccg gaaaaaggat tggaaaaagt cattgctgac
      481 ggagaaaaaa atcttacgca ggaaaaacta gatgaaatct ccttgacaga tgatgtgctt
      541 tacgatcagt acggcgaaaa tatctatgat acttctaaaa aattgcataa aacagccaaa
      601 atatggggaa cagatgattt tggcggcttt ggtttcttaa attttgtttt tgaaggacta
      661 gtatctggag ataagtatgg atctgctgta gggatcgctg cattgatcct tgttgttgga
      721 ggtgcttttg gtattattat gcgtaccgga gcgatcgacg caggtatcta tgcgtttatc
      781 agtaaaacta agggactgga gcgtttagct ttaccgctat tattctttgc tttttcattc
      841 ggtggcgcta cattcgggat ggcagaagag gtaattccat tctcaatggt gatggttcca
      901 tttgtcattg cgttaggata tgactcgatc gtagcagtaa ccgtgactta tgtcgcatct
      961 caagtcggaa atgcgacctc ttggatgagc ccgtttagcg tagcggtggc tcaagggatt
     1021 gctggaatcc ccgtgttgtc tggtgcgaca ttccgattga ttatgtgggt agttgttact
     1081 gcattagccg ctggttatat gatgatatat gcagaaaaaa tccgtaaaaa accagaaagt
     1141 tcgttgactt acaaatcaga tgattatttc cgttctcata tcaaaaagac atctgatgaa
     1201 aataaaaaat tcatgcttgg gcataaattg atcttattag agatgttagt tgtccttgtc
     1261 tggatcgttt ggggcgtgac tcaaaaaggc tattacattc cagaaatcgc ttctcaattc
     1321 tttgtgatgg gattagttgc tggaatcatt gctgttcttt tcaaattaga tggaatgaaa
     1381 gtcaatgaca tcgcttcttc tttccaatct ggtgcagctg atttagctgg tacagcaatc
     1441 gtagtaggta tggccaaagg gattcttctt gtcttgggag gatctgatgc atctgttcct
     1501 tcagctttga atacgatttt gcatgggatc gggacggcgt tgaccggtgt tccagctgtc
     1561 attggtgctt gggcgatgta catcttccaa agtttgttca acttggttgt tacatcaaat
     1621 tcaggacaag cagctttgac tatgccaatc atggctccac tggcagattt agtaggtgta
     1681 tccagacaag tagcagttct tgcttatcag cttggttcag gttttatgga cgcttttaca
     1741 cccgtatcag ccagcttgat tggtgtgcta ggtgttgcac ggatcgaatg ggcaaaatgg
     1801 gcaagattcc aaatcaagat gcaaggattc ctgtttgttt taggaactat tttcatcatg
     1861 attgccattg caataggtct gcagtagtac tggagacctg taaataaaaa agtaatccaa
     1921 acagtagata aggactccaa gaaccgtcat tttacggtaa aatcaaagga gtaaagatcc
     1981 tatcgtttgg attatttttt aagatcctat catcagttat acttaaagaa agaggaaaaa
     2041 tgtatgaaaa tcattcgaca aattgaagtg tttgcaccag attacttagg aaaaatggat
     2101 gtactgattg ccggtgggaa gattttggct gtagaagaac agctagaagg cggctacgaa
     2161 ggagtggaag tagaagaact ttcaggtgag ggaaaagtac taaccccggg tttcattgac
     2221 tgtcatttcc atattttagg tggaggagga gaaggcggtt atcaaaatcg aacgccagaa
     2281 gtcacattga gccaattgac tacagctggt gtaacgacag ttgtcggttg tttgggaaca
     2341 gacggagaag gtagagatat gactgcgttg atcagcaaag cgaaaggtct ggaagcagaa
     2401 ggaatctcca cttatgtata cgaaggttcg taccgattgc cggtaaaaac agtaacagat
     2461 tctatcatca aagacttttt gacaattgat aaaattatcg gaatcggaga aatcgcagta
     2521 tccgatcacc gttcttctca gccaagtttt gaagagttcg ctcgtgcagt agcagatgct
     2581 cgtgtaggag gcatgttatc cggaaaagct gggatcatca atgtccactt aggcggagga
     2641 aaaagaaagc tagagctatt gacacgaatc gtagaagaaa cagaaattcc gatcacacaa
     2701 tttttgccta ctcatgcaaa tcgcacacca gaattatttg aagcttgcgt ggcatttgcc
     2761 aaacgtggtg gcacgattga cttcactgct agcgaagatc ctgatttttg ggagaagaca
     2821 gatggtgaag ttcgcttcag caaagcactg aaacgattga tcgaggaaga tgtttcttta
     2881 gataatttca cgatgacctc agatggacaa ggaagcttgc cttattttga tgaaaacaat
     2941 catttcctag ggttaggagt aggaagtgcc aaggcattgc tagtagggat caaagaagct
     3001 gtgcaaaaag aaagtatccc tttggaaatc gccttacgag ctataaccag taatccagca
     3061 cgtatattga aattggataa aaaaggaaaa atcgaaattg gtgctgatgc agatctgtgt
     3121 atcttggata aagaaacgct tgatattgat acagttatcg ctaaaggaga aataatggtc
     3181 caagaaaaag aagtcaaagt atgggggact ttcgaaaaaa gtttttaatc aacagctcta
     3241 actatctttt ttagaaaacg agcgttctgt ttgtctgtat ctgttgttga tgttaccctg
     3301 aaggtagatc aataaacaaa ctaagaggag gaagcacaat ggcagattta aaaggacgtt
     3361 ctaaagatat gaaagataaa gtagtcggtg aaggaaaaga agcatacggc aaagcaacag
     3421 acgacaagag caaagaaaca gaagggaaag cacaatcact aggtgctgac atcaaagaaa
     3481 aagctcgaaa tttaggcgac gaagtcaaag acaagttttc tcgaaaagac gaaaaaaaat
     3541 aaaaaccact tattactaat tgaatcaagc aaataaaaaa tccgaaccat atctgcgaga
     3601 cagatatggt tcggattttt tatttgatca ttatcctgtt ttttaaactt cgattttttg
     3661 aaatttttta ttttttagtt caaagacttc atcacagctt tttgctactt caatctcatg
     3721 agtcacgatg ataatacagc gattttgttc atgggcgatt tcttggaaaa gtttgacgat
     3781 atcttgtgaa gttgtttcat ctagatttcc agtaggctca tccgctacga ttagttgatg
     3841 ttcacagcag atagcacgaa cgatagcgac tcgctgctgc tgacctcccg aaagtttggt
     3901 cacctgtttt tttgctaata tttcatcaat tcctacacga gccagtcgat ctaaagcaac
     3961 ttgtcgataa ttctcttgct tggtcttagt gatttccatt gctgctatga cgttgtctag
     4021 agccgataaa taaggaagca agttgtaagc ttggaaaata atcgagacat catttttacg
     4081 ataacttctt aacccgattt tgttcagtga acgatcttga tagtatacct ttccttcttt
     4141 tggtgcatct agtccagaaa taagcgaaag aaaagtcgtt tttccagaac cacttgctcc
     4201 taaaatagcg tacatttttc cagcttcgaa attcagttct acatctttat aaagcgcatc
     4261 ttgcggatct tgataccaaa atcccaattg ttctgtccgt aacatcaaat tcctccttta
     4321 gtttaatagg atttttcttg ggtttaatct taatatccct atagaagcta aaaagacaga
     4381 aaacagactg attccaaatg ctactccggc taatagtccc aattgtgcag gtttgacatt
     4441 gatttccaat tcgctgactt gatcagaaac agcaaatgga ttattcgtgg ttccttgttg
     4501 accatcgcgt tgaccgcctt gttcttgtct gttacctggc atttgtccac cgccaggagc
     4561 ttgctggttt tgtgtcgtgg tttctgtctg ttggtcaagt agctgctgtc ccacggcatt
     4621 cgcgacgacg tttccgctaa gcgaagcaac tcctaatgca agaatcatac aaatagccac
     4681 ttcggtaaac aattggagaa tgattttact tctggattct cctaaagata gcaagacgcc
     4741 taactcatgc cgacgctccc gaatggtaat cataatgatc agagtaagta tgataatacc
     4801 ggctacagcc actagtaaaa caacattttt agaaaaactt gccacgttgt ttaaaggttc
     4861 caacattgac tgatacatgg aatcatttga ttgtaagcta aatgtatctg tgtcgatcag
     4921 cttctcagct tctttgagaa actcatccat tttatctgga tcagatagag tgtaaatagc
     4981 agaatctatc gtgttatcgt cagatgtccc atttagttca tttgcaaaag tgtaagaagt
     5041 gtaaagtgta ttcgacggat tcatgaagtt gaatttcata cccatactgc cgcttgtttc
     5101 gctggattca taaattcctt tgatcgtcat ttcataagtg gtatcttcat cttcagtact
     5161 cgtgattgtg aaagtatctc cgactgccag gtcatttgtt tctgctagag tagaatcaat
     5221 gactacattg tttgtgcctt tgtcgctgtc atctattcct tcaccatccg tgatttttgc
     5281 tgttccatca gaaaaattac tattttgtgc agttgctgac acaccactga tttgaaagtc
     5341 tgcttgagtc atttgcggat tcccgcccat tcctccagca ggttcaccgc ctgcatcagt
     5401 agtttctgaa ctgtcttcac ttgaatcaga actagtgatg gcagttattc catcttttgc
     5461 catagctgag gcggaacttt caaaggaata actctttaca ttatctaatt cagctatttt
     5521 ttccgcgtcc gttaaggaaa ctggtgtaag agaaaaactg cctggatctg gacgttcttc
     5581 agtggtagaa ccgcttgaag aagtttgttc ctgtttttta aatgaagctt ctcgattggc
     5641 acttaaagtc actgttgctc caacttcttt ttgtgcttga ttcgctgctt cttctgcagc
     5701 gcttcgaatc gtgaggccag ctagcacaaa aataagaatg gctgtgaaca cggcaaacaa
     5761 taacaatgaa cgtccccatt ttacttttgt acttttccat gcacgtttca taaaattcat
     5821 attttgcatc cctcctagta gatgcatttg ataatagaca acctatctta aagctagctt
     5881 aaatcttatg ctgtttgcac accatcaaca tgaggttctg tatatataaa aagtcactat
     5941 agttattgca aaaaatgtgg taaaatgaaa aaagtatgtt tcaaaccgac gaagaagaaa
     6001 tgaaagagga atagcaaaag aaatcctaga tgctacagag ggatttttac gtgcatagtt
     6061 gttctaacaa ttgctgatac tagcgattta gtttatttgt tgtataataa tggggaacag
     6121 ctgaatactt atataagtat tctggctata gcgtttcgca aaagatccat tttgttaatg
     6181 aagtatatcc aaaataaaat gcccatccaa ctgtcagaac gcggatgtgc tggtaatttt
     6241 cctgctgatt ctccataagt tgagaaatag tgattttgca tatgcttcat tggaatggct
     6301 catatattta gataaagaag aaaatcgaca tctttaaact tggatgtcat aagaagaggt
     6361 gaagtttgtg agtaccatta aaattatccc attaggcggc gttcgtgaaa atggaaaaaa
     6421 catgtatgtc gcagaagtgg gcgaggatat ttttatttta gattgtgggc taaaatatcc
     6481 tgaaaatgag ttgttgggga tcgacgtcgt aatccctgat tttacgtatt tagaagaaaa
     6541 cattgatcgt gtagctggtg tctttttaac acatggacat gcagatgcga tcggggcact
     6601 tccttacttg ttgtcaaaaa tttctgtacc agtattcggg acagagttga cgatcgagtt
     6661 agcaaaattg aatgtcagtc gtaatgaagc agccaaagga tttaaggatt tccatgtggt
     6721 agatgagcat acagaaatag actttggtga aacagtagtg agctttttcc gtaccactca
     6781 tacgatacca gattcagtgg ggatcaatct gaaaacaaaa gaaggtagta tcgtttatac
     6841 gggagatttt aaatttgacc aaacggctat cccaatgtat caaacagatt atgcacgatt
     6901 ggcggaaatc ggcaaagaag gagtcttagc cttactaagt gaatcggcaa atgcagaaaa
     6961 tcctcagcct gtagcttctg aagcccaaat agcagatgaa gtatttgata cgatcaaata
     7021 ttgggaaggg cgtatcatcg tcgcttgtgt agcaagtaat ttgcaacgag tgcaacaagt
     7081 attggatgca gcagaaaaag cacaacgaaa agttgtcttg actggtcaag atttcgaacg
     7141 aattattcgc acagcgatgc gtttagaaaa acttcaatta ccaagtgaag atctgcttgt
     7201 tacgttgaaa gacatgaaaa actatgatcc agaagaactg atcattttag aaactggccg
     7261 aatgggtgaa cccatcaagt ctctgcaaaa aatggcaaac ggtacacacc gtaatgtcaa
     7321 gattgaagaa ggagatttgg tttatatcac tactacgcca agcatcgcga tggaaaccat
     7381 cgtggcgaaa acggaagata tcatttatcg agctggtgga acagtgaagt tgatctctga
     7441 gaatatgcgc gtttctggcc atgccaatcc taatgacttg caattaatga tcaatttgat
     7501 gaaaccaaca tattttgtgc cagttcaagg agagtatcgt gaattagctg ctcatgcaga
     7561 tttggctcat gcagtaggga tgccatacaa aaatatctat attactggac gcggagacgt
     7621 gctggaatat aagaacaagc gtatgagtgt tgcaggaact acaagtgcag aaaatgtaat
     7681 gattgatgga ttaggcatag gtgatatcgg aaacattgtt ttacgtgatc gaaaaatctt
     7741 gtcagaagac gggattttcg tagcggttgt cactatcagt cggcgagaaa agaaaatcat
     7801 ttcaaaacct caaattactt cacggggatt cgtttacgta aaagctagcc gtgatctgat
     7861 gaaagaaagc agcaatatga ttgaaacaat cgttgagaaa catttagaaa gcaatgattt
     7921 tgagtggagc aaattaaagc aagacatccg agatcaatta ggccgttact tatttgaaca
     7981 aacaaaacgc cgcccagtga tcttgccagt cattatggaa gctactcaac gtcgaaataa
     8041 aaaataaaca aaaagagtaa gatgaacctc tctatagagt gctcgtcttg ctcttttttt
     8101 gcgaaaaaca aataattttt gaatacagca tccctcgctt tataataaac gatagaataa
     8161 agggagtgaa tcaatgatga gtcaaacaaa agtcgaacgt tatttagaag aacaaaagat
     8221 cagctatcaa ccacttgatt ttagtcatgt ggtagaagaa tctttcacag aagaactaca
     8281 aaaaagaggg attgatccag ctttgatatg taaaactctc gtagtgaaag gaaacaaaac
     8341 tggggtgatc atcgcattga ttcctttgaa cgatcactta gattacaaaa aaacgagaca
     8401 agtgacgaaa gatcgtaaag ttggttttcc tggaatggat tttgtattaa tgcatacagg
     8461 atatccccac ggagcaaaca cccctattgg aataaaactc gcacatcctg attattgttt
     8521 tttagcagac agttctttaa aagaaaaaga agaagtaatt gtgtcaagcg gtgaaatcgg
     8581 gagaagcctt cggataaaag tagccgattt agaaaagatc gtccatccta tttacgctga
     8641 tttaattagt taagggacga gaagagaagc taagcagagc agagacctca aacttgagat
     8701 tatatctgct tggtacttgg tgcatcctgt attatctgtt acaattgctt tgtacatact
     8761 gtactatcaa tccaatgaaa atatatttag cagctatatt ttttcattga tgtgcaagtg
     8821 atgaaatcgt ttttaataaa aaagatacag agaaaacgcg aggtgtaaaa tatgaaaatt
     8881 acgtatcatg gacattcctg tttaacaatt gaaatggaaa atggccaacg cgtgatgatt
     8941 gacccgttta ttacaggaaa tccattgaca gacttaactg cagaagaagt aaagacagat
     9001 tggttgctga ttactcatgg tcatagtgac cacataggag atatgattcc tattgcaaaa
     9061 gaaaatcaag ccacaattat cagtattgtt gagattgctg attatgcgaa cagcagagga
     9121 gtagattcat tcggtatgaa tatcggtgga aaacatgcgt ttccattcgg cgtggtaaaa
     9181 ttcgtacatg cacagcatag ttcaagctat gaagtagacg gtatcgttca gtatatgggc
     9241 gagcctagcg gaattattat tcaagcagaa ggaaaaacga tttatcatgc aggggatact
     9301 gcttatttca gtgatctagg attgcttgca gaagaattcg atattgatgt cgctttcttg
     9361 ccgattggcg ataactatac gatggggcca gaagatgcag caaaggctgc gcaaacaatt
     9421 cgtgccaaga aagtagttcc aatccattac aatactttcc ctgtcattga acaagatccc
     9481 gaatcttttg tagagttact ggaagatagt gaaggaaaaa taatgagcgt ctcggaaacg
     9541 ctggaagtgt aggcagaaaa tggcgacaaa acatgatttg attttagaat atattgaaag
     9601 tttacctgtt ggcaatcgaa tttctgtaag aagcatcgca aaagaattga atgtcagtga
     9661 aggaacagcg taccgtgcaa tcaaagatgc agaaaatgtc ggactagttt caacaattca
     9721 acgagtaggg accatccgta tcgaacgcaa attaaaaaaa catattgaac gtttgacatt
     9781 tggcgaggtc gtcaggatca ttgaagggga cgttttaggc ggggcggcag gattagacaa
     9841 ggtcttgaat aaattcgtca tcggcgcgat gactaaacat gccatggaac gctatattac
     9901 gccaggatca ctaatgatcg tcggcaaccg ggcggaagtc caaaaattag cattggaaga
     9961 tggcgccgca gtattgatca caggaggatt caatacgact gctgagattg caaagctagc
    10021 tgatgatctt gaaatgccga tcttacgcac ctcttatgat acatttacag tcgcaaccat
    10081 gatcaaccga gcgttaagcg accaattgat caaaaaagat atcatgcttg tcagtgatat
    10141 ctatatgcca gctgaaaaga ctcattattt gcagcagaca gatacgattt ctgattatca
    10201 gcgtttgtca gaagaaacgc agcattcgcg ttttccagtc gtcaaccgcc atcatcgttt
    10261 gatggggatt gttacggcaa aagatgtttt agggaaatcg ccaaaccaga tcatcgatcg
    10321 ggtgatgacg aaagaaccaa tcagtgtaaa gaaaacaatg agtattgcct ctgttagcca
    10381 tcaaatgatc tgggatgggc tagaagtcat gccggtcgtt tccgatgact tgacattgga
    10441 aggcttgatt acccggcaag atgtaatgaa agcgatgcag cttgtccagc gacaaccgca
    10501 gattgcagat acgatttcgg atcagatatc tggaaatatc catacgattg atactgaccg
    10561 tgaaggaaat cgtttggaaa accctaagtt caaattcgta gtagcaccac aaatggtcaa
    10621 tggcgttgga accatttctt ttggcgtact tagcgaaatc atttctgatg tcgcacaaaa
    10681 aacaatggtg atgaaccaaa aacgaaatat cttgattgaa caagtgaacc tacattattt
    10741 gcggctgatc caattggaaa gcgaattaga cattcgtcca cgtgtattgg aaatcggcag
    10801 acgttccgct aaactggata tcgaagtgtt tattgaaaac accatcgttt ccaaagcaat
    10861 cgttgtttgt caagtaatgg aacgttctta gaaagcagga gtgaaatgac agtacagcaa
    10921 gaaatagtag atacaataaa aaaatacgat aaaatcatta tccacaggca tttgcgccct
    10981 gatcctgatg cattaggatc acaagtagga ttagccgaac tattacggga aagtttccca
    11041 gaaaaagaaa tacgtcaagt aggtgaaacg attgaagggc ttcgcttttt agcagaaatg
    11101 caagaagttg aagatgtctt ctaccaagga gcgctagtca tcgtgactga tacagctaat
    11161 tcacccagaa tcagtgatca aagatatcag ttggcagaaa aactgatcaa aatcgaccat
    11221 catcccaatg atgaccctta tggagatctt gtgtgggtaa atacaaaagc aagtagctgt
    11281 agtgagatga ttgcagaatt tgctttgatg tttcctgaat ctttaaagat gaacgttaag
    11341 gctgcacggt tactttatgc aggaatcgtc ggagatacag ggcgcttttt gtatccctcc
    11401 acgacgtccc gtacattaga gattgcttcg atattgagaa attatccatt tgatgcaagt
    11461 gctttgaaca gagagattga acaaatgccg atgaaagtcg ctaaactttc cggttatctt
    11521 tatcaaaata tccaggtgga tgaaaatggt gcaggaagaa taatccttcc gcaagaaatt
    11581 cttgataaat atggaatcga tgattcagag acagcttcga tcgtttcact tcctggagtg
    11641 attgacgaag tgctggcttg ggggattttt gttcagcagc cttcagggta ttaccgcgta
    11701 cgtttacgtt ctaaggggcc gatcatcaat gaattggcta aacgccatca tggaggaggc
    11761 catccattag caagtggtgc gaatgcaaaa gatttagcag aagtagagga aatctataaa
    11821 gagatccaag atatatgcca atcgtataag agcagaaaaa cagaatgata cttcgttcga
    11881 aaacgtgcct tttgtagtaa aaatcacaaa tgtatctttt caagatcttc tttttccgtt
    11941 acaatagtgc tcactaaaga ttttaaagga gaaagaagat gacattacca aattgcccag
    12001 aatgtggctc agagtacaca tatgaagatc gaggcttgtt gatttgccca gaatgcggga
    12061 acgaatggaa caaagaagaa caagaaactg tcatagaaga cggattagtg gtattagatg
    12121 caaatggaaa ccgtctgtta gagggcgata gcgtgacagt gattaaagac ttgaaagtaa
    12181 aaggtgcgtc aggtgccatt aaacaaggaa caaaagtcaa gaacatccgt ttagtagaag
    12241 gcgatcataa tatcgattgc aaggtagaag gatttggacc gatgaaactg aaatcggaat
    12301 ttgtgaaaaa gaattaaaga atattcaagg tcttttcaca ttcttttcct actactaatg
    12361 ataaaataaa gtaaacgaaa aggagtgaaa cgaaaatgta tggttatgga atgtatggtt
    12421 ttttagatcc gacatttctt ttggtcattc ttggattagt tatttccgga attgcgtcag
    12481 cgtacgtcaa tagtactttc cgaaaatacg atcaagtgag aagcaaaaac aatgtgactg
    12541 gtacacaagc agcacaatat atattacaaa gtcaacagat aaataatgtc ggtgttcaac
    12601 aaattgccgg agatctcaca gataactaca attcaggtaa taagatgttg agtttgtctc
    12661 aagcaactgc acagtctact tcagttgctg cgataggtgt agcagctcat gaatgcggac
    12721 atgcggtaca agatgcttcg ggttatgtcc cattgaagat tcgtgcagca cttgttccag
    12781 cagctaactt tggttcgatg atttcgttcc ccttgattat ggtaggtgta ttgcttagct
    12841 ggaactctac gttaatcaat atcgggattt tcgctttttc tttagcatta cttttccaat
    12901 tggtgacatt accagtcgaa tttaacgcgt ctcggcgagc aatccaaatt ttgagtgaag
    12961 gcggactact gacggaagaa gaagtaccga tggcaagaca cgtattattt gcagcagcac
    13021 tgacttatgt tgcagcagcg ctttcgacat tcctgcagct gttgcgcttg atcttattgt
    13081 tcggcggcaa ccgaagagat taaaataaag aagttgtgaa atcagcgctt tgatctgagc
    13141 taataccgag agttagctga ttgaatacca tttatttgga ataagaggat gtgacaagaa
    13201 ttttgtcaca tcctctttgt tttgttctca taaccttttt tcatatacag ttcatgaacc
    13261 gcttttatta acttgccaaa taacactgta ttgtgcgttg tatccagata gaaagtctcc
    13321 gtttcatctg aaattttctc atgctgatct gtaatgatca aatcagcatg ttctgaatat
    13381 gcggtaattt taacagcatc tttgcgaaat aactgctgca aatggctttg aagatgataa
    13441 tttcctgtaa actgtttggt tatctgcaaa tggatgagta actgctcttt cgtttctgtt
    13501 tgtagtaagt gttggatcaa ttgactaagg tattgactga tttggtttct ttccccattt
    13561 tttgaaaaaa ttgtatcgat tttttctctt aatttctttt cctgtataaa agaactagaa
    13621 tgattggacg tggcttctgg aaagcaaagt gtagaaaaac tactgctgga ttcacccaac
    13681 agttcgaaaa aaacaactgc caacacaaga aaataaaaat aaattggtat tttattttct
    13741 aaatggttga gaggaaactg ttgagttaag taagtcatca attgaccgac ttgtcgacaa
    13801 ataaggtttt tgcttgtgga aaataaaaaa ccaatctcct gtttttgtcg aaaagagatt
    13861 tgatttggaa aaaaacaata gaaaaaataa ttaaagtagg ctctctctgt ttctgtctct
    13921 tcacaagggc gactcactga aaaagaagta aagtattctg aataatcatg tgtatgctgc
    13981 aataagtcca tcaattcagt gatttcagaa gaagtttttg gaaaatatgc ctgttgtttg
    14041 tttcgtaata tgaataaagt taacagcaag tagatatttt tttcttgttt tgaaattttt
    14101 tctatttgct catctgtact gttcattttt agtgcattta attcaagtga ggaaaatggc
    14161 caatcgttac ctccaaatgt atctgaaaga aaaaagtaaa gatagagacg gatagataac
    14221 tcatttcctt caagagtaca gcaatttttt acagatagga tccgtaaatg gaattttatt
    14281 aaggaatgat tgagctgttt tgtcagtttt tcgagatagg agggcgatat ataaagttgt
    14341 tcaagtaaat atttttttgg tacagcagtt tgtggataat tgattagtaa catcaaatag
    14401 ttaaaacgat tggattcttt taggtagcat aattttaact tagaaaaaac agctaacttt
    14461 ttagtttgat cggtttcttt ggtttggatc tctcctttat gattagaata aataaattca
    14521 tcaatcaata aaaattgctt gagtgataca tttatttttc ggatcagttc tttgattgtc
    14581 cgttttggaa tatgtaagtc attggataat tcttgtaaag aacaattatt tttttgaatg
    14641 accatttgta gaacaagtaa ctcttttttt cttgcttcaa ttagcataaa tacctccttt
    14701 ttattctgtt tttgtatata gttagacaaa aactgtgagt ggaaagtcat ttcagtctgt
    14761 gttattctca cagagtgtcg ccttcttttt tatataacgt tatttaattt ttttgttacc
    14821 ttgtttattc tattgtttat ttcctagggt attttgtcaa gatattcttg tttttaaggc
    14881 ggaaaaattg atttttaatg gatataacta aatatgtttt gataattatc aataatattt
    14941 ttagaattat ttatgttatt tatttatggg tagatgaaaa gagacggagg taagaatgat
    15001 ggggagagta acaaattata gtaaagaata tttgctgttc aaaagtatgg tatacgtgga
    15061 agaatatgga atgaagtcaa ttactgcaag agagttagca gagttctgcg gttgttctac
    15121 atacccgatt tatacacatt tcaaatcaat cagcgggtta aaagagaaga tattagaaga
    15181 aatcactgtt tattttgaaa actacttagc tgaatgcagt tccgatgatg ttttaagcat
    15241 tgtctactta ttgaaagatt ttttttctat ccatgaaagt attcgtgaaa tcattgcaaa
    15301 atctgattta gacatggatt cgctatttaa gcggccattt ttctcatatg tcaaaggtca
    15361 cacgaagatc aaagaagaaa agtcgcttga attggtatgg cttaatgcat tgggaagttt
    15421 gcgctcagat gattcttctc aagattttct tgaaatgatg aacgctgttt gggataaaaa
    15481 cgaggtgacg gatgaatact tggaaaatat cttaaaggaa cagtcacctt aggcagcaac
    15541 atgaaaaagt agatcagcca cctttatttt tcgtaaaaaa gattgtaaaa tgtctaacga
    15601 aacattggaa tgaaggagga agcgacaacg ttaccacagc aatttatcaa aaaatatcgt
    15661 cttcttttag gcgaagaagc atccgatttt ttttctgcgc ttgaacaagg aagcgtgaaa
    15721 aaaggatttc ggtggaatcc actaaaaccg gcaggattgg acatggtcca aacttaccat
    15781 agcgaagaat tacaacccgc tccctacagc aatgagggat ttcttggcac tgtcaatgga
    15841 aagtctttcc tccatcaagc tgggtatgaa tatagccaag aaccaagtgc aatgatcgtt
    15901 ggaacagctg ctgcagcaaa acccggtgaa aaagttttgg atttgtgtgc ggctcccggt
    15961 ggtaaatcaa cccaattagc tgctcaaatg aaggggaaag ggttattagt gacgaatgag
    16021 atctttccga aacgtgcgaa gattttatca gaaaatatag aacgatgggg agtttcgaac
    16081 gcaatagtga caaatcatgc acccgcagaa cttgtccctc atttttcggg cttttttgat
    16141 cggatcgtgg ttgatgctcc ttgttcagga gaaggaatgt tccgaaaaga tccaaatgca
    16201 ataaaagagt ggacagaaga atctccgcta tactgtcaaa aacgtcagca agagattttg
    16261 tcttcagcaa taaaaatgct aaaaaataaa ggacaactga tttattcgac ttgtacgttt
    16321 gcaccagaag aaaatgaaga gatcattagc tggcttgtag aaaattatcc agtaacgatt
    16381 gaggagattc ctttgactca atccgttagt tcaggacgtt ctgaatgggg aagcgtagct
    16441 ggtttggaaa aaacaatacg tatatggcct cataaagatc agggtgaagg acattttgtt
    16501 gcaaagctga cttttcatgg acaaaatcag atgcataaag aaaagaagac acgaaaaaaa
    16561 agtaaagtgc aaatgacaaa agagcaggaa aaactctgga cggagttttc aaatgatttt
    16621 cattatgaag ctactggaag acttttagtg ttcaacgacc atctttggga agttcctgag
    16681 ttagcccctt ctctcgatgg tttaaaagtc gtacgtacag gtttgcattt aggagatttc
    16741 aaaaagaatc gatttgaacc tagctatgca ttagcgcttg ctacaaaaaa gatagagaat
    16801 ataccttgct tgccgatcac tcaaaaggaa tggcaatctt acacagcagg agaaacattt
    16861 caacgtgatg ggaatcaagg atgggttttg ctagtattag ataagatacc tgtcggattt
    16921 gggaaacaag taaaaggaac agtaaagaac tttttcccta aaggattgag atttcactga
    16981 gtggattagt tcgtttattt ttttgtctat gctatgatgg agatagataa aaaggagctg
    17041 atacttatga aaacatcaac aaaagtaaca atcggattaa gcgtagcagc cgctgccagt
    17101 gtagcgacag cagtcgttgt atcgggaaaa gtaatggaaa agatccatca tatgactact
    17161 cggacgaaag taaaaaaatt cgtctatgac aaatttgatg gaaatgaaaa attactagaa
    17221 atcgtagatg gtctttcaga tagtgacttg gattctttga tgggaatgtt agcaaaaatc
    17281 aaatcaggaa aaaagaaaat ctctgtttat ggtgattcaa tcaaagattc aaccgaagat
    17341 gtaaaagacc ggctaatgca ttttgttgaa aaaatgatgt aagagaaact agcttttgaa
    17401 cactattcat tcgaaataag gggttgtgac aaaattattg tcacaacccc tttaatctat
    17461 ctttaattat gttcatgaag caataaaaga tagcaaaaca gtaaatcgtt ggcttttttt
    17521 agattgaaac cagactgttc ttgaaatttt tcgattcgat attttagagt attacgatga
    17581 agaaacagtg tttttgcagc agagctgaca ttgcctaatt cattccataa ttcttgaatg
    17641 atcacttcca tttcattttg tttgatcagt tctatgtaac ttttgatcaa cttactatct
    17701 tttacagtat cttttgtata gtaatgtaaa gccacatctt gcatagaaaa agtacggctg
    17761 tgagtataaa gattgttttg ctctgttaaa aaaatttttc tttcttcagc aaacaaaggg
    17821 acgaggtcgg tgccaggtga atggaaagaa ccaacgaata cagacgttgt cgtatcaaaa
    17881 tcagcatcta aagatagaaa aataccgttg atttcagtag catcaagata atcacggttt
    17941 atttgttcga ctaagagcgc atctgtgtct gtataaaaaa agaaatcagc agaatagcga
    18001 aataattcca aaatagtatc ttgccattct ttttttaaga attctccttt tgattcgacg
    18061 tgaagctgga tcaaacgaaa gcttttttct tgaacacaag aagcatcttt aaaaagatag
    18121 tgataccatg ggtgtttttt ttgatcgttg attacaggga ataacgcttt caaaaggctg
    18181 gtttcttgtt gagataatga atcttgtttg atccagataa aagcatcatc aattgcaaaa
    18241 gaaaggatat ccgcttctga tgctggagag ttttttttct gtgcttgtgg gtaaagagca
    18301 agtaatttat ccattttcat cttgttcacc tcttgtattt attttaccat agaaaaaaag
    18361 ttagttacag cctttttatc atatgctaaa gtaaaacaag aaaaaacaaa ggcagggaag
    18421 ggtaattggt aaatacaaat cttgatgcag tagaaaaaga aaaatggatg cgccttgcat
    18481 tggcagaagc aaagaaagca gaaatgcttc atgaagttcc gattggagca gtggttgtat
    18541 tggatggaaa agtgattgga agaggatata accttagaga aaccacgcaa gatgctacta
    18601 cacatgcaga aatgcttgca atcaaggaag cctgtgaaaa agtgggaagc tggcgcttgg
    18661 aagatgctgc actttttgtt accttagagc cttgtcctat gtgcagcggc gcgatgattt
    18721 tgtctcgtgt agcagaagtt tatttcggcg cgtatgatcc aaaaggcgga acagccggta
    18781 ccttgatgaa tcttttggag gacgagcggt ttaaccacca agcatatgta gaaggtggca
    18841 ttttagaaga cgaatgtggg atgattttga ctgacttttt ccgaaagctc cgccagcgaa
    18901 aaaagaaatt aaaaaaagac tggtcaacga atgaaaaata aggtatacta ttctttgccg
    18961 aaaggctagg acaatggtgg gcttgtgaag cgtgtcaggt ctggaaggaa gcagcactaa
    19021 gcaggttccg ccatgtgtct gattataatg aaacgacaac agactttcgg gtctgttttt
    19081 tttattttta tagtactgta aaaatgaatg ttcctgattt tttagccaga ctgatttatc
    19141 gaatattgta cttatacaat ttgcttcaag gcaggaaaaa ctctgaaata gagcttgttt
    19201 caatggaaga aaaagctgcc tatggtatga taaagataaa gagaggcgag agggtacatg
    19261 gaaaaaatag tgattttaca tacgaatgat ctgcattctc atttagaaaa ttggccaagg
    19321 atcagaaggt ttttggatca acgtaaacga gaaaacgaaa agaaagaaaa aacgacaacg
    19381 attgcagtag atctaggtga ttttgtggat cgttggcatc cattgtcaga agcaacgaat
    19441 ggacaggaaa atatcttgct gatgaatgaa gttggttatg atgcagcgac gataggaaac
    19501 aatgaaggcg taggaaattc caaagaagag ttgaatcatt tatacgatca tgctgagttt
    19561 gatatcatcc tggataactt attcgataaa aatacgctcc agccaccggc atggacgaaa
    19621 ccatataaaa tcattacaac gaaacaccag acgaaaatcg gactaattgc atttactgct
    19681 ccatttccgc ttacttataa tccgaatggc tgggacatac ggaatcctta tgatattttg
    19741 ccagaattag tcgaagattt acgttcacaa gtggatgttt tagttttgat gagtcatctc
    19801 gggattcagg atgatttcca aattgcaaaa gaaataccag caattgatgt tattttagga
    19861 tcgcacaccc atcatttatt cagagaaggt cacgtaatca atgatgtcca aatagcagcc
    19921 gctggaaagt acgggcagta tgtcggtgaa gtccatttaa ccatcgatga agagaaaaaa
    19981 atccttaagc attcagccaa agccatccct accgaaacaa tgacagcttt tacagaagat
    20041 gaacaagaaa taaatagttg tctagaacgt gggcatgaat tattaaaaga aaaagaagta
    20101 gcagatttgc cattttctct gagtctagat atcttcaatg agcattcgtt tatccaagta
    20161 gcctttgagg cggtgaaaga acgaggaaag acggaagcag caatattaaa cagtggtctg
    20221 tttttaactg aaattccaaa agggcgggtc aatcaggatc agcttcacac tgcgttgccg
    20281 catccgatgc acttgttaaa tgtcacattg ggtggaaatg atctgatccg cttagtttta
    20341 gaaatagaaa aaaatcgtaa ctttttacgt aactatccta tgaagggaat gggatttcgc
    20401 ggtaagattt ttgggcagat ggtctataat ggaatcacat atgattctat taaccatcaa
    20461 gtcttttgga agaacaagcc aatcgatctg aataaaaatt atacttttac taccgtagat
    20521 catttgatgt ttgttccatt ttttccaacg attgagattg ctggaaaaaa tgaatttttg
    20581 ttccctgaat ttatccgaag tgtggtagga gattacttga aagctcacta cccaatcaaa
    20641 taaaaacaag gtataataaa aaagataggt ggtgaacgca tgtctgcaaa acaaaatgaa
    20701 cagcagaacg ttgagcataa aacagaaatt gctaagaaaa aatcatcagc tgaacaggaa
    20761 acaacagtca ccgaagaact gacagctgct ttggaagaaa tcgttgagga gcctaaagca
    20821 gaaaaagtca aaggcgagct agtgacactg ttagatgaca gtaatttttt gataggtgaa
    20881 agacattatc ggcttgtgtc cgattatcgt gaaggattta atgcagaaaa gcttggagaa
    20941 cgttacagtg atgtactagc ccgatatgat tatatcgtag gagattgggg ttatgaacaa
    21001 ctacgcctaa aaggattttt ccaagcggat aatcgacggg cgcatccaga tcagcgaatc
    21061 gattccttgg aagactatct ttacgaatat tgcaattttg gttgtgcgta ttttgtcatc
    21121 gagagaatcg gcggtaaaaa agaaaagaca actcagcgcc gaaaaaagaa aaaaaaccat
    21181 aatcgtgcgt atatcgatga gaaaaaagga ccagtcgcat ctaatcgcaa aaaaccggtt
    21241 attaaaaatc gaaaaacaga gtcagcaaaa acctctgaaa aagtaaaaaa acaagaacaa
    21301 cctaaacaag tagaaaaaac aaaaacagca tttacgatca gaaaaaggga agagtagtgg
    21361 atgaatggac tataaaggat atttaattga tttagacgga acgatttatc gtgggacaca
    21421 acccataccg gccggtaaac gatttgtgga agaattgcaa aaaagaaaat tacctttctt
    21481 gtttgttacc aataacacga ctaagtcacc agaaacagta gctaatcgtc tagcagatga
    21541 gtttgacatt catgtagctc cagagacagt ctacacagct actttggcaa caatcgattt
    21601 tatgaaagca gatggaaaag ggaaaaaagt ctatgttatc ggagaagcag gattgattga
    21661 cttgatttta gctgccggat ttacatggga agaagaggct cctgactatg tagtggtagg
    21721 cttagataat tatctgacct atgaaaaagt agttaaagca acacttgcta ttcaaaaagg
    21781 tgctactttc attggaacga acccagataa gaatatacct actgaacgcg gccttcttcc
    21841 tggagcaggg tcagtcatct cttttgtaga gacagcaacc caaacgccgc cgatctacat
    21901 cggaaaacca gaagccatca ttatggataa agccgttgaa gtattagggc tgcaaaaaga
    21961 agaagtaatc atggttggag ataactatga aacagatatc caagctggga tacgcaataa
    22021 tatagatact ttgctcgttt tatcaggttt tacaaaagaa gaagatgtac ctgggttgcc
    22081 tatcccagct acctatgtga aacaatcact ggatgaatgg agcttctaat atgaaaaatt
    22141 cgagatggca gtggatggaa tatgctggat tgttcagcct atttctgaca ctgatcagtc
    22201 tggcagttgg tgtgacgatc aactttcgac cactatacgt atttgatatc ggacatttgc
    22261 aaattttgga ttatacttct ttagatcaag agacgttact gaaaaatttt gatcacctaa
    22321 tgaattatct caataatccc ttcaaaacca tcctcagctt gccagatttc ccagtttctg
    22381 ctagcggagc ccatcacttt tatgaagtga aaattttgtt tttagtggat tatgctgtgt
    22441 ttttcatcac tttgatcccg agtattttgt ttatcaaata tttgcaaaag aatgatcggc
    22501 tttggcggtt gattcgtccg tttcaaatcg gcatgttact gccagttgtt ttcggttttt
    22561 tcatgatgat aggatttgat cgctttttta tcctttttca tgaaacgttt ttcaacaacg
    22621 atgactggtt attcgatccc gttactgatc caatcatcaa tgtacttcca gaagaatttt
    22681 tcatgcatag ctttatctta ttttttgtat tattagaatt attttttgct gtttttcttt
    22741 tcttaggaaa aaatagcttg aaacaaacaa aaaagaaaga actcgtctaa atagacgttt
    22801 tctttctttt tttgttgaat tattgagtaa ggcgacttct tttttctggt tctttgtgtg
    22861 ctaatcgact ggcagcagca gcggcgatcg ctccgacgat atcatctaag aaggtatgga
    22921 catgaacgcc atcgtggctg tttagttctt ttaaaatacc aggtttcacc ttgtcgatat
    22981 aaccataatt cgtaaagccg atcgtaccgt aaacattcac gattgacaag gctaggatct
    23041 catcgatacc gtaaaggcct tcgtcatctt cgataatatg ttgcaacgga ctcatcaatt
    23101 ttttcttttc tgctaatata tctaattgga ttccagtgat aatggcgtta tgtacttcac
    23161 gctttgttag cacgctgttg acactttcag tacaatccgc caaagtcaaa tcagaaatat
    23221 agtctttttg taaaaacatg acaagttgtg cgatatcttc tacagttacg ccacgttcct
    23281 tcaatagttc tcttgccttt tcttctaaag tctcagtttt gacgaccata ttattgctcc
    23341 ccctttattt tttctttctc tatttcatgt taacggaaca tgaaaaaaat gcaagaaact
    23401 gctttcgggt ttaacttgaa atgcaaagag atataaaaaa tcaactagga cgaagatttt
    23461 tatcgtataa gcttgtgctg tgaccaggct gagttcgttc ctttggatta atatagtgta
    23521 aagcgttgtt cactgcagta ggtgcttcac cgagcccagt agcaatcagt ttcacttttc
    23581 cttcataggt acagatgtca ccagctgcat aaacgcctgg aatagaagta gacatatctg
    23641 attttacagt aatggcattt ctggtactat caagtcccca agaagaaaga tgttctaaat
    23701 tagaagagaa tccataattg acgatcagag aatcaatcat caaatcaatc gtctcgtcac
    23761 ttttcacttt ttttaaccgg atatctgtca gttccccatt attgcctgat aatccatcga
    23821 tgagataggg agtaagcaag ttgacagatg aagatttcaa gcgtgataca ctatgttcgt
    23881 gcgcacgaaa ttccgggcga cgatgaatca agtaaacttc gcttgcaata ggctctaaca
    23941 tcaacgtcca atcgatagca gaatcaccac ctccagcaat agcgactttt tttccagcat
    24001 acttcatcaa atcattcacg aaataatcca atccgtggtt ttcaaatgat tcagcattat
    24061 ctaaattcaa ttttcttggc tggaatgaac catttcctaa agctaaaatg actgctttgg
    24121 aataatggat gcctttattt gtagtaatct cgataacttc gtcttcacga gtaagcgaga
    24181 gaacttcttc ttttaaatgg aatgtatggt taaaagtagt taattgtttt tctaattgat
    24241 cgattaattc acttgctttt attgctggat acccaggaat atcgtaaata tatttttcgg
    24301 gatacaaagt tgctaattga ccaccaagtt gaggtagact gtcaatgatt tttgttttag
    24361 cttggcgcat tcctgcgtaa aaagcggcaa acatccctac aggacctgca ccgatgattg
    24421 taatgtcata gatttccata aaaattcctc ccttttcaca ctatatcaga atttaattga
    24481 gaatgcttct caaaaatgtt tgaattcgaa atcgaaacgg taagttatag aatgtatcta
    24541 gtgatgaaaa ataaaagaaa acaaatctag tatttttacg agaggttagg tattatgtta
    24601 ttattattat gaagtatgca aatattagga ggaaacaata tggcattccc acaactagat
    24661 ttaaaaagtg aaaaaggacc aaaagctgtt atcaaaacaa accgtggtga cattactgtt
    24721 caattattcc ctgaactagc accgaagacg gttcaaaatt tcattgaact ttccaaaaaa
    24781 ggttattatg atggcgtgat ttttcaccgc gttattcctg atttcatgat ccaaggcggc
    24841 gatccaactg gaacaggaat gggtggcgaa agtatttatg gcgaaagttt tgaagatgaa
    24901 ttcagtcgtg aactgttcaa ccttcgcgga gctttatcta tggcaaattc aggtccaaac
    24961 acaaacggaa gccaattctt tattgtaaat aacacaaatg tgccagctaa tatgcttgga
    25021 caactagaag gtgctggatt cccatcagaa atcattgaag cgtataaagg tggcggaact
    25081 ccttggctag atttccgaca tacagtcttt ggacatgtac tagaagggat ggacacagta
    25141 gatgaaatcg caaatgtaca acgtggtcca caagaccgac ctgtacatga tgtagtgatt
    25201 gaaacaattg aaatttcaga atagtttggt atggtacgga gtaattatga aaaaggacga
    25261 aacctagtaa ggtctcgtcc ttttattttg aataaatgat ttaatcgatt gtctctaaag
    25321 catacttgat tcgattaagt ccttccagta aagtttcttc tggacaagca atattcaagc
    25381 gcatatgccc atgaccggaa ggaccaaacg tgatgccagg atttaatacg acttttcctt
    25441 tccgaacaag agtgttctcc aattcttggt cgtctgaaat ataggcggta aaatctaacc
    25501 acattaagta agttccttca gggttcatta ttctcacttt cggaagatac tgttcaaaaa
    25561 actgattaac tgtttgaaca tttttttcta gataaggaat cagttggctt aaccattctc
    25621 cacccgtctc ataagccgct tgggttccaa tcagtccaaa agtgttgatt tcgtgctgtt
    25681 ggttcatcac taaatgtttt tcaaaatcag ctttcatctc tggatttttg ataaatacca
    25741 ttgaattttt gattgcagct agattgaaag tcttggttgc ggaagtaaaa gcaactagta
    25801 gatcatctaa attaggatca atcgttagca tggaagtaaa tgtatgatcg aataaagtta
    25861 aatcttgatg gatctcgtca ctaaacagaa tcacttgatg ttttaaacac aattcgctta
    25921 actgtttcaa ttcttctttg ctccaaacac gccctccagg attgtgagga ttgcataaaa
    25981 tcattgcttt tacattgttt tcttcgattt ttttctccat gtcagacaag tccatgatga
    26041 aatgattatt tttttctagc aaagagcttc gaacgatgtg acgttgattt gtttctataa
    26101 tagaggaaaa tggaggataa actgggtcat gaatcaaaat agaatcgcct ggttttgtaa
    26161 aagtttgtac tgctgcagct aaactggtta acacaccact tgtaaaaacg atattttctt
    26221 tcgtcaactc cacagcatgg tgttggcgtt cccaatgaat gatagcctga tatagtttat
    26281 cagacagagt ggcataacca aagattccat gtttgatgta ttcactaaac gctttagcta
    26341 ccccaccggg agacaagaag tccatatctg ctacccacaa aggaagtaag tcatccattt
    26401 gatatgtctc agcaattgcg tcccatttta cactttctgt atcttttctt gaaatctgtt
    26461 tatcaaattc gatcatctta agacctccag tcattttcgt cgcttctttt tttgagctat
    26521 ttacagtata atgataaatg cgaagagagg gaagtagatg acttataaaa ttggacaaat
    26581 aattgaagga aaagtgacag ggatacagcc ttatggcgca ttcgtttctc ttgataacga
    26641 gacgcaagga cttattcatg tttcagaagt acaatccggt tatacgaaaa atatccattc
    26701 attgttaaaa gtcggacagc cagtaacagt tcagatcatc gacattgatg aatattccaa
    26761 aaaaatcagt ttatctttaa gaacgttaga aaaaacaaat ccagctgttc catatcgcag
    26821 aaaaaggtat ttcacgaata aaaatcgtaa gatcggattt accactattg cggagcagtt
    26881 acctatttgg atagaagaag cattatctga actagaacgg aaagaaaata agaaataaaa
    26941 agtgtatttt ttctgaaaat gtgctagaat ttttatgtaa gccatagaac aagttgatcg
    27001 aggtggaaat tttggcaagc aaaagagttg ctggtacaat tatgttgcat ttggaggatg
    27061 gtactaaaaa atttttggtc cattcaattg atgacaagtt ggaatttgcg ttagcggaac
    27121 tttctgaagg aaagactggt ttagcgaata tacttaattt cttaaaagaa atagttcata
    27181 tcgatgttag caagattagt ttgatggaat tgacaaatac acatattaaa gaggaaaatg
    27241 ttccgttgtt tgtctttgaa actgaacaaa agaatcaaag agaagaatta ggcgaaggat
    27301 atatttggga agaaccaggt caaatgcgct ctgttcttgg aacttacgaa gtagagggag
    27361 tgcctttctt ctaaaataca gattaagcga acaaagaagt ttgaattttt ttcaaacttc
    27421 tttttttatt gtttggtcca aaaaatacaa tgaaatgttc tgctaaagat agttttttag
    27481 tgcattttat tttcaaaata tgttgaaaac ggttgtataa cgaataataa taattgatcg
    27541 aaaaatcatt tcacaaagta atgcttaatt tatagttgac caaggaagaa atacatgata
    27601 aatttaacag cgttgattct gaagtggatg ttatatcaca tttctaaaaa atttaaaaaa
    27661 tagttattga catgttacta acgacatgat aagataactg agt
//
LOCUS       DAZBTF010000032        27666 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000032 DAZBTF010000000
VERSION     DAZBTF010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.032"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="ACHGNS_001452"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="ACHGNS_001452"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFN6937404.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="ACHGNS_001453"
     CDS             complement(937..1518)
                     /locus_tag="ACHGNS_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN6937405.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="ACHGNS_001454"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="ACHGNS_001454"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFN6937406.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="ACHGNS_001455"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="ACHGNS_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFN6937407.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="ACHGNS_001456"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="ACHGNS_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFN6937408.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="ACHGNS_001457"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="ACHGNS_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFN6937409.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="ACHGNS_001458"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="ACHGNS_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFN6937410.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="ACHGNS_001459"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="ACHGNS_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFN6937411.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="ACHGNS_001460"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="ACHGNS_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFN6937412.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="ACHGNS_001461"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="ACHGNS_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFN6937413.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="ACHGNS_001462"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="ACHGNS_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFN6937414.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="ACHGNS_001463"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="ACHGNS_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFN6937415.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="ACHGNS_001464"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="ACHGNS_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFN6937416.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="ACHGNS_001465"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="ACHGNS_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFN6937417.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="ACHGNS_001466"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="ACHGNS_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFN6937418.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="ACHGNS_001467"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="ACHGNS_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFN6937419.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="ACHGNS_001468"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="ACHGNS_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFN6937420.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="ACHGNS_001469"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="ACHGNS_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFN6937421.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="ACHGNS_001470"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="ACHGNS_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFN6937422.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="ACHGNS_001471"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="ACHGNS_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFN6937423.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="ACHGNS_001472"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="ACHGNS_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFN6937424.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="ACHGNS_001473"
     CDS             13972..14157
                     /locus_tag="ACHGNS_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFN6937425.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="ACHGNS_001474"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="ACHGNS_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFN6937426.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="ACHGNS_001475"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="ACHGNS_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFN6937427.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="ACHGNS_001476"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="ACHGNS_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFN6937428.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="ACHGNS_001477"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="ACHGNS_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFN6937429.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="ACHGNS_001478"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="ACHGNS_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFN6937430.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="ACHGNS_001479"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="ACHGNS_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFN6937431.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="ACHGNS_001480"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="ACHGNS_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFN6937432.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="ACHGNS_001481"
     CDS             18232..18879
                     /locus_tag="ACHGNS_001481"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFN6937433.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="ACHGNS_001482"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="ACHGNS_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFN6937434.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="ACHGNS_001483"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="ACHGNS_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFN6937435.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="ACHGNS_001484"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="ACHGNS_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFN6937436.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="ACHGNS_001485"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="ACHGNS_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFN6937437.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="ACHGNS_001486"
     CDS             20317..21255
                     /locus_tag="ACHGNS_001486"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFN6937438.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="ACHGNS_001487"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="ACHGNS_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFN6937439.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="ACHGNS_001488"
     CDS             21935..22774
                     /locus_tag="ACHGNS_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFN6937440.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="ACHGNS_001489"
     CDS             22750..23619
                     /locus_tag="ACHGNS_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFN6937441.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="ACHGNS_001490"
     CDS             23609..24406
                     /locus_tag="ACHGNS_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFN6937442.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24609..25355
                     /gene="truA"
                     /locus_tag="ACHGNS_001491"
     CDS             24609..25355
                     /gene="truA"
                     /locus_tag="ACHGNS_001491"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFN6937443.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            26019..26213
                     /locus_tag="ACHGNS_001492"
                     /pseudo
     CDS             26019..26213
                     /locus_tag="ACHGNS_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(26401..27177)
                     /locus_tag="ACHGNS_001493"
     CDS             complement(26401..27177)
                     /locus_tag="ACHGNS_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFN6937444.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
     gene            27300..>27666
                     /locus_tag="ACHGNS_001494"
     CDS             27300..>27666
                     /locus_tag="ACHGNS_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HFN6937445.1"
                     /translation="MLDQTDINIIEELSKNSRITMKELGEKVHLTGPAVSARVTKLEE
                     SGVIENYTVKLNMEKLGFSIHAFLIIITQNLNHKPYLSFIKNHEKFLIRNYKISGGGC
                     YLLECRFTSKKSMNSFLEQL"
BASE COUNT         8996 a         5062 c         5843 g         7765 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggt attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttgaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtagggttt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatgg tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggta gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttgaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcgagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    24541 agaacgaaaa aattttataa aaaaatcatt attccagata accttttagg aacaaaggag
    24601 agaaaaacat ggtacgttac aaagcaatca ttgcatacga cggaacaaat tttaatggat
    24661 tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa
    24721 tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg
    24781 cgattgggca agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt
    24841 tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag
    24901 aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag
    24961 ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac cctcttgatt
    25021 tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt
    25081 gtgcatcagg aagctcgata gaggataaag tacgtacgat ctacgaggct aagatggagg
    25141 taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga
    25201 tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata
    25261 gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacatcctg
    25321 aaggacttta tttgtatgaa gtgaaatatg aataagaggt tgtgacagaa gtggtcagct
    25381 tcaagaaata agaaggaatt tacgaaaatt acttctcaaa attttgcaaa atttcggctt
    25441 attttcgaag gagctacttc tggaacaccg tgaatcagag gttgtgaaaa agctgccctg
    25501 catcaagtaa taagaacaac caaacaaaaa tagctcgtca tatttttcgt ttggttgggc
    25561 ttattaccgc agatgcaagc ttttgaacac cgtttaagga agaggttgtg aaatcagcgt
    25621 tttgacctga gtattagaga aacaaacgga aaaatagttc ctcatatttt gccgtttgtt
    25681 gagctaatac cgaaggtcag ctgattgaac accgtttaag aaagaggtcg tgagcctgtc
    25741 atttaactct gagtcacaag taacaatttc aaaaaacagc ttctcatctt tgatgaatat
    25801 cgtgacttgt ggacgaggcg ttgataagtg aatcatcttt ttgttaccta ttttttctgt
    25861 aaccaaaaaa cgatggaacg ttgttttgat tgcccttaat gaggacagtc ggaacaggtt
    25921 tgtcattttt atatttaaat ggcacaaatt tttctaatta tttgtcattt agcgctgaaa
    25981 atttttgcta tttatctaaa aaagtgataa ctacttaaga tgaagcagaa tatttacgcc
    26041 atacggcata caaccgcgaa aaatataaaa aaaagaaaac gattgaacgc gtttttgcag
    26101 tcgcaaagga aaagcatgga atgcgctaca tcaagtatcg agacttggaa aacaatcagg
    26161 tgtatacgct gattgttttt gctgcaatga atctgaaaaa gttgtccact tggcagttgg
    26221 aacatatgtc ttcatatctt ttattataca aaaatagcaa aagcaccagt ctacactctt
    26281 agtaaagagg aaactgatcc ttttgtctac attcttaaag tacttaaact aagtacttta
    26341 ttttactctt taggattgat acagtagcat ctatcccttt attaataatc ttttattttt
    26401 ttaaataaag atgttgtctc catcttcagg cactaaaact ttttcggaaa ttcctttttc
    26461 tttattgaaa gactttaatt cttctcttga cagtccccaa tggtttacag cttccatatg
    26521 aacggcaata attgtggaat ctggtattgc tttatgcaat tcatataaat cgtctttccc
    26581 cataataaga gattctccaa ctaaaaaatg attatctcca gcatttgcta ctatgatttc
    26641 tggattatac tgatcaatag tttgtttaac agcttcatac caaactgtat ctcctgtaaa
    26701 gtatagtgtc ttctcagtcg aatgtttgaa aacaataccg caaacattac ctacataatt
    26761 caagatttct cctctgccat gtttgccttt tgttttaacc aattgtatag attcaaaaac
    26821 tgtgtcttct cgtagtacct ccacattttt aaaaccatat agtcgaattt cattcgcatc
    26881 attttcatct tgcacaaata attttatttc tttaggtagg acctctttcg ctatatcgtc
    26941 ccaatgatct agatgtaaat gtgttacaat aacagcatca ataccgttga taatatcttc
    27001 gactggcatt ggcaaatcaa ctagaggatt catttgatct tttcttggtg atggaaaagg
    27061 acctttcgaa ataaatggtg gaagtgttcc tttttccgct aacattggat caattaaaaa
    27121 ttttttacct ccatagtcta agatagctgt tgcattacga atgtgatgta tattcataaa
    27181 cgttgtctcc tttttgttga tatgaaatat ttttagtata attcaattcc atacaataaa
    27241 taaaccgaac aaaaaaagtc ttttactatt tttacttaat ttatgaaagg ttgtttacca
    27301 tgttagacca aacagatata aatatcattg aggaactatc aaaaaatagt cgaatcacaa
    27361 tgaaagaact tggagaaaaa gtacatttga ctggacccgc tgtctcggcg agagtgacaa
    27421 aactagagga aagtggagtt attgaaaatt atacagtaaa attaaatatg gaaaagctag
    27481 ggttttcgat tcacgctttt ttaattataa ttacgcaaaa tttaaatcat aaaccctatt
    27541 tatcatttat taaaaatcat gaaaaattct tgattagaaa ttataagatt agtggagggg
    27601 gctgttatct tttggaatgt agatttactt caaaaaaatc aatgaacagc tttttggaac
    27661 agctta
//
LOCUS       DAZBTF010000033        27565 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000033 DAZBTF010000000
VERSION     DAZBTF010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.033"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            49..696
                     /locus_tag="ACHGNS_001495"
     CDS             49..696
                     /locus_tag="ACHGNS_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFN6937446.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            780..1724
                     /locus_tag="ACHGNS_001496"
     CDS             780..1724
                     /locus_tag="ACHGNS_001496"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFN6937447.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            1859..3310
                     /locus_tag="ACHGNS_001497"
     CDS             1859..3310
                     /locus_tag="ACHGNS_001497"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFN6937448.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            3331..4167
                     /locus_tag="ACHGNS_001498"
     CDS             3331..4167
                     /locus_tag="ACHGNS_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFN6937449.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            4167..4826
                     /locus_tag="ACHGNS_001499"
     CDS             4167..4826
                     /locus_tag="ACHGNS_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFN6937450.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            4952..5641
                     /locus_tag="ACHGNS_001500"
     CDS             4952..5641
                     /locus_tag="ACHGNS_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937451.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            5647..6603
                     /locus_tag="ACHGNS_001501"
     CDS             5647..6603
                     /locus_tag="ACHGNS_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFN6937452.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            6647..7159
                     /locus_tag="ACHGNS_001502"
     CDS             6647..7159
                     /locus_tag="ACHGNS_001502"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6937453.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(7415..7963)
                     /locus_tag="ACHGNS_001503"
     CDS             complement(7415..7963)
                     /locus_tag="ACHGNS_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFN6937454.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(8052..9233)
                     /locus_tag="ACHGNS_001504"
     CDS             complement(8052..9233)
                     /locus_tag="ACHGNS_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFN6937455.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(9469..10977)
                     /locus_tag="ACHGNS_001505"
     CDS             complement(9469..10977)
                     /locus_tag="ACHGNS_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFN6937456.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            11136..11327
                     /locus_tag="ACHGNS_001506"
     CDS             11136..11327
                     /locus_tag="ACHGNS_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937457.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            11530..12447
                     /locus_tag="ACHGNS_001507"
     CDS             11530..12447
                     /locus_tag="ACHGNS_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFN6937458.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            12440..13513
                     /locus_tag="ACHGNS_001508"
     CDS             12440..13513
                     /locus_tag="ACHGNS_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937459.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            13661..14179
                     /locus_tag="ACHGNS_001509"
     CDS             13661..14179
                     /locus_tag="ACHGNS_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937460.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            14160..14552
                     /locus_tag="ACHGNS_001510"
     CDS             14160..14552
                     /locus_tag="ACHGNS_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFN6937461.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            14628..15563
                     /locus_tag="ACHGNS_001511"
     CDS             14628..15563
                     /locus_tag="ACHGNS_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFN6937462.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            15696..16475
                     /locus_tag="ACHGNS_001512"
     CDS             15696..16475
                     /locus_tag="ACHGNS_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFN6937463.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            16468..16785
                     /locus_tag="ACHGNS_001513"
     CDS             16468..16785
                     /locus_tag="ACHGNS_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFN6937464.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            17029..18639
                     /locus_tag="ACHGNS_001514"
     CDS             17029..18639
                     /locus_tag="ACHGNS_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFN6937465.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            18796..19644
                     /locus_tag="ACHGNS_001515"
     CDS             18796..19644
                     /locus_tag="ACHGNS_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFN6937466.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            19875..20354
                     /locus_tag="ACHGNS_001516"
     CDS             19875..20354
                     /locus_tag="ACHGNS_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFN6937467.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            20387..22615
                     /locus_tag="ACHGNS_001517"
     CDS             20387..22615
                     /locus_tag="ACHGNS_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN6937468.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            22744..24387
                     /locus_tag="ACHGNS_001518"
     CDS             22744..24387
                     /locus_tag="ACHGNS_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFN6937469.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(24460..25005)
                     /locus_tag="ACHGNS_001519"
     CDS             complement(24460..25005)
                     /locus_tag="ACHGNS_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFN6937470.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(25028..25513)
                     /locus_tag="ACHGNS_001520"
     CDS             complement(25028..25513)
                     /locus_tag="ACHGNS_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFN6937471.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(25503..26315)
                     /locus_tag="ACHGNS_001521"
     CDS             complement(25503..26315)
                     /locus_tag="ACHGNS_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFN6937472.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(26454..>27565)
                     /locus_tag="ACHGNS_001522"
     CDS             complement(26454..>27565)
                     /locus_tag="ACHGNS_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFN6937473.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         9029 a         5021 c         5521 g         7994 t
ORIGIN      
        1 gttatacttg taatagaata ctattttata acaaagggga tcgaatcaat gaaaaaaact
       61 gttatttata ctagtttatt tgcagctttg tttttagctg cttgtcaatc aaataccaat
      121 caaggcacat ctgggaatac gtcttccagt agcgaacagc gctcttcaca ggtatcggac
      181 tcctcaacaa aagcaagcac atcttcgtct agccagacaa aaagttctag tgaagcaagt
      241 actcagtcag atatcaaagt gtctgcagct cgtgcgatcg aattgttcca agataaatac
      301 ccagatactg caattactag tttggaatta gatagtgatt ggggatctta cttctacaag
      361 atagaaggtg tcgatgatca aaaagaatac gaagcaagaa ttgatgctca aaatgaaaaa
      421 atggagacag agaatccaga aactctagat cgagatgaac aaaatggtca aaagaaaaaa
      481 gaagatggtc tggatgttga taaattgatc acgattgagg aagcagcaaa aatcgccgtc
      541 gacaaagtag gaagcggtac ggcaacagat tgggatttgg ataaagattt aggtactact
      601 tattgggaag tcaaagtaaa agacggcaat caaagtacat cagtcaagat caatagccaa
      661 acaggcgaag tactgtcaac tgaacaagat gattaagaat ttcttcggaa aacaagagtt
      721 gatttttctt ttagtcatga tacactagac aaaataactt gtttgaagga gcgatttact
      781 tgagtttaga aacgaccttt gccaatcata tctttgccaa tcctttgatg aatgcatctg
      841 gtgtccactg catgactacc caggaattag atgaactggc tcattcagaa gcaggagcat
      901 ttatcactaa gagctgtacg atcaatgaac gaaaaggaaa tcccgaacca cgctattttg
      961 atgttccttt agggagtatc aattctatgg gacttccaaa cttaggtttt tcttattatc
     1021 tagagtatgc tcttgcgtat gaaaaagtgc aggaaaacca aaatcaaccg ttattttttt
     1081 ccattgccgg gatgagtgtc caggaaaact tggaaatgct tgagaaaata gaaaaaagtg
     1141 ggtttaatgg gatcactgaa ttaaatttat cttgtcccaa tgtgcctgga aaaccacaac
     1201 tagcttacga ttttgaggcc acttatgaaa cactgaaaga agtattcagc atcttttcta
     1261 agccgctagg aatcaaattg cctccttatt ttgattttgc tcattttgat cagatggctg
     1321 atatcttgaa tcagtttcca ttgacctatg tcaatgctat caacagtgtg ggcaatggtt
     1381 tgtatatcga tacagaacaa gaagcagtag tcatcaaacc aaaagaaggg ttcggcggca
     1441 ttggcggaga gtatatcaag ccgactgctt tggcgaatgt tcgcgctttt tatacacgtt
     1501 tgaaaccaga aatccaaatc attggtacag gtgggattcg gacaggacaa gatgcatttg
     1561 aacatctgct atgtggcgca agcatgttac agattggtac cgagcttcat aaagaagggc
     1621 cggaaatttt ttctagaatc ataaaagaat taacgcagat tatgtcagaa aaaggatata
     1681 cgtcaattga cgaatttaaa ggcaaattac gcaccatttc atagcaaaga gacgaaggtc
     1741 atgattgatc ttcgtctttt tttataaaaa acactcgtct gttcattcct gtgcaagcgt
     1801 actcattgta tgttagacga acttgtggga aaataacaga ggcaaaaagg aggataatat
     1861 gaatgatgca acagaattag caaaagagct taaagaaaaa gaatttagtt ttcaagaact
     1921 agttacggat atacagaaaa aaataaagaa gcaaaatcat gaattgaatg cgtttgtcgc
     1981 gcttgaacca ttcgagagta attcccacga gctattgatt ccccagtcag aaaaagggcc
     2041 attggctggt atcccatttc ctttaaaaat gctcggtcaa gaaaaaaagg gatggctagc
     2101 tacttcagga tctcgtttat ttgaaacaca ccgagcttct catacatcta attatgtgcg
     2161 gcaagcagaa gcgataggtc tagttccttt tggacaaacc aatgcgccag agtttggttt
     2221 caaaaatatt acagacccag tcatctatgg cccagcaaga aacccttgga accttgaatg
     2281 tacaccgggc ggatcaagtg gaggagctgc tgctgcagtt tcatcaggaa tcgttccttt
     2341 agctggtgct agtgatggcg gtggttctat tagaataccc gcttcgtttt gcggattgat
     2401 tgggctgaaa ccttcgcgag ggacgatgcc agttggtcca tatgcttgga gaggatggca
     2461 aggagctgct attgatttcg gcttgacagt ttctatgaga gacacggagg ctttatttta
     2521 tgggatgaga agtatccatt ccggtgcgcc atatcaagta tcgccagttg agtggcaaac
     2581 acatccaaga aaaaaacggt tgaagatagc tttttgtgtt gattcaccga tccactcaca
     2641 gattagtgaa gaggcagtac ttgctgtaaa aaaagctgta gatttccttg gaaaagaagg
     2701 gcatgatttg acagagattc cttacccact agatggtcgt aaactgatca cgtcttatta
     2761 tcagatgaat ggcgcagaaa cagcagcaat gatccgctcg attgaaacag gcatccagcg
     2821 agctgtcaga aaagaagaac ttgaacccat ttcttggact ttgttccaat atggtcagaa
     2881 gctctctgcc gctacttaca tcgattctct gcatgtatgg gatgaagcag caattgtcat
     2941 ggaaaaccta catcaagaat acgatctatt cctttcacca acgactgcat ttaccgcccc
     3001 taaaatcagc gaagatttgc aaagtgatgc gataagagca cagatggaac acatcagtga
     3061 ttgttctgaa gtagaaggtt tagacgttgt ttcttccatg tttgaggaga gcttgaagat
     3121 cacaccttat acacagttgg ctaatctgac tggacaacca gcaatcagct tgcctactca
     3181 tttgacaaaa gaaaacatgc ctctaggaat ccaatttatg gctgccagag gaagagaaga
     3241 tttgctattt caaatcggct atctatttga agcaaatcat cagttccatt taccaaatcc
     3301 atggaaatag cagaaacaag gagaagaaga atgaagaaaa aaagattgtt tgctctaata
     3361 actagtgcct tttttatttt aagcgcttgc ggtacagctg ccgctaatca ggaagataac
     3421 agctggagta atgtagaaaa aaagaaagaa ttagtagtag cgacttctgg cacactgttt
     3481 cccgcatctt attataatga tcaaaatgaa ttagttggtt atgatgtaga tttggcaaaa
     3541 gaggtggcta aacggttaaa tgtcaaaatc tcctttaaag aatacaacgt ggatggacaa
     3601 atcacttctg tttcaaaaag agaatcagat tttgctgcga acgattttgg tatcacaaaa
     3661 gaaagagaaa aaaaattctc cctttcagag actttcaaat attcatttga cagtatgatc
     3721 gtacgaaaaa aagatgattc aggaatccac tcacttgaag atcttaaagg gaaaaaagca
     3781 gctggcgaac ccaatactag ctatatgcgt ttagcggaaa aacacggagc taaattagtc
     3841 acatatgaca atgccacgaa tgatcagtat ttaagcgatg tagcaaatgg tcgaacagat
     3901 gttattctaa atgattatta tctgcagaaa atggccgtat ctgctttacc agatatccct
     3961 gtaaaaatac tggagaacgt ttatttcaat gaaaacgaaa gtggtttttt attgaaagaa
     4021 ggaaatgatg ctctgaaaaa gaaaatcgat ggcgtattaa aagagatgaa agaagatggg
     4081 acgacaaaga aaatatctga aaaatatttt caaactgatg tttcagtcaa accgaaagaa
     4141 acgatggctg agtcggtgag tgagtgatgt actttcctct aattgatttt tccctcatgt
     4201 ggacttcact tcctttcgta ctgcaaggct tggtatacac attagggatt ggcttcgtgt
     4261 cgtttctttt aggaaatctg gtagggttgc ttttgacagt tttaggtctt ttggattggc
     4321 tgcctctgaa tgtatttatt cgtttttatt tatcgttctt tagaggcatt cccgcactag
     4381 tcctgctgtt tctgttatat tttgggttac cttatcaatt aagtgcctta acggcttcag
     4441 tcatctgctt tacgattacc agcagtgcat ttattggtga aatctatcga ggatcactag
     4501 caggcgtttc ttcaggtcag tgggacgctg gtttagcttc tggtttttct ttctttgggg
     4561 tattgaaata tatcgttttg ccgcaagctt ttcgaatctc agtaccggca ctcagtaacg
     4621 tagctatgga tctagtgaaa ggtacttcgt tagcagcaac aatcacagtg ccagaaatat
     4681 ttcaaaaagc aaagatcata ggcggtcgga cattcgatta tttttctctc tatattttag
     4741 ttgcactgat ttattgggtc atttgtatcg ggatcgaaaa agtgcaacag tatttagaaa
     4801 aacgttctat gcatcagtca atctgaaaaa tagaaatggt gctagcgtgg ctggcaccat
     4861 ttctattttt tgtttcttgt atattctgcc tagtatttta aaaccatttc atgtaaaatg
     4921 taaatcgaaa atatgttcgg aggtagaaaa catgaagacc ccgctaacca tcgaaataga
     4981 atcctcgtta taccaatatt gcatcgagca aggcggcttg gttgtagaag aagtcacgat
     5041 gcctgatgac caaggaattg tagatacgct tagctgcttg acaaaatttg atggaagccg
     5101 agaatggcga tgctatgagc tgaaagtgac aaaagcagat tttcgctcta cagccaaact
     5161 gtcatttgtt ggacattata attattttgt tttaccaagt aaactatatg aagaagtaaa
     5221 aactgaaatc cctacaggaa taggtgtttt agtgtatcgt ccgtacacgg ttgaagagga
     5281 gatgcctgcc tctggtacat ttctgattgc gaaaaaagca gcaaagaaag agcttgctgt
     5341 accggaagaa gctttgacaa accgcttcat ggcttcttta ttcagggaag taagaaaagc
     5401 aaagcaaatg gagcgaggcg taaaatattt tccaacagat caattgtaca aagaattgaa
     5461 aagacgttca gaaaatcaag atccattttc taacgaacga tattatgaac ggtttttaga
     5521 ggaaatagct agtcaggcaa tcaatgagtt gcaagaagaa gttatcgcgc ttcagcatga
     5581 ttatgaatat ttgaggcaac aacagcagat ccgtcggttg cctacagagc cattggagtg
     5641 aaaaatatgt actatcctta tttaagagga aaacaatttg acctattagc attaaaagaa
     5701 gcactgtcgc gaggattgct ctccaataaa atccaaccag tgatcgaacc agtacgtgat
     5761 tccgctacgc ttaaaaatgt gatcgaatta tttcaaaaaa agcaccatcg aatcgctgtg
     5821 atccaaaatc cccaagtagg acaattcaaa ttattcgatc agcatgtgca cagttggaca
     5881 gtcaaagaaa atagctctgt cgtccctgca cagatcttaa cgccagaaaa tctcagtgaa
     5941 gtactggatt cacctccagc gtttcttgtt ttcgatggac agcactaccc aaaagataca
     6001 gaggtgtgga aacagttggc aggagttgat tcgaaatttt tgataccaga tactagtcgc
     6061 tttcgcatct ggctgcccga aaacaaaatt gtgatccgtg actcttttca aacgagaaag
     6121 catgttgaaa gttatgcaga taaaacagat gactttttta gtgatgacta tctttttttc
     6181 cacgcagacg gatatattgg attttctgat ttcacgatcg aaggtagccg ttatttcgat
     6241 aaagggttcc ctagccgagc gcttgctatc catctaacgt atattgatgc ttacggcaat
     6301 atacgcgtga aacattttgt ctctgacagt aacgactctg ctaaagacca agcattgaag
     6361 tttcttgaag caggagataa aatgaaaaaa tggatcatgc gtcaccatca tcaactcttg
     6421 attacttcag gaatgattga attattggct ctttaccagc aacaaaaatt ccctggactg
     6481 ggagtgttga aaaagtggtc acttatgcat catttggaac ttattagtca cctgctagat
     6541 catccaaaag attggcttaa atattattca aaagttcagg aactatatga agtgatccaa
     6601 tgattagata atgtaaatga aaaaggaata gaaaagagga aactggatgt atattcggca
     6661 aacaaggaaa gaagaagtag aagaagtggt agaaatcatt aatgccgcag cggatttcat
     6721 tgcttcgcaa ggctctcctc aatggcaaaa cgggcagaaa ccaacaaaag aaacgatttt
     6781 gaacgatatt gaaagaaaag aaagctatgt attaatagtg gaagatcaaa ttgctggaac
     6841 agctgcatta gtctcaagcg tggatcccgt ttacacaaaa atagatggta gttggaaaga
     6901 atcaactggt ccttatttat cgattcatcg tgtcgcttta gcagaaagta ctcgaggaaa
     6961 aaaactagca aaaccgttct tagaacattt gttgacgatt gcagccagca aaaatatcca
     7021 agatatccgt atcgatactt atccagctaa tgaaccgatg gaaaagacca tctattctgc
     7081 tggattcact tatcaaggga tgattgaatt tccttttgaa cacggcgaaa gaaaagcata
     7141 tcaaataatc atcaattgac tataaaatca tctgagcaaa gataaagaca ttaacactac
     7201 tgaaaaaaac aatcatatga tagccaagag gttgtaacag aagtggccag cttcaaaaaa
     7261 caagaaagaa tttccgaaaa tgcttctcaa atttttgtgg aattttagcg tattttacga
     7321 aatagctgct ttctgaaact cccttcatcg agaggtcgtg acatagcttt tgtcacgacc
     7381 tctcggctta tcctacgagt attattcgat acatttatat caataatcct ttttttattt
     7441 tatctgcagc ttcttggaac gctccatcgt ttgacacgac taaaagtgag gggatagctt
     7501 tcaaacgttt caactgtgct aaatcttcta cataaaccgc cagtctggct gaaatctgtt
     7561 cagatgaatc tccacgtttt ttcaggcgcg attgcaatgt ttctttagag atcgtaataa
     7621 agataggacg gactaccgtc ccgaaatact catataaatg ccaaaaacca tttgctgtca
     7681 aaacggcgta acaatttcct actgtcagtt tctttttaat ttctgcttta ctggaaccat
     7741 atttatgttg agcatattgg tcccactctg caaactcatt tcccttgatc atctgattga
     7801 atgtattgtc agaaacaaaa taataatcct cttgatcttt ttccccaggt cttggtgatc
     7861 gattcgtgta agtaatgatc ttttgttccg gctgaaaaat agcttctgcg agtgaagttt
     7921 ttccagaacc agaaggaccg ataaatacat agcaataatt cattgtcatt tctcctttga
     7981 tgattgaaca agtgaaaaag ctttggaaaa tatttccaaa gcttttaaaa gtaactttgc
     8041 ttttttgtat attataccag caagccagtc acaatagcac ttaaaaagct gactaaagtc
     8101 gctgttagca aaattttcaa tccgtgtttt gcgacaagat ttcctttttc ttcatttaat
     8161 cccttcatcg caccagaaat gatcccgata gaagagaagt tggcaaatga aacgaggaag
     8221 acagatataa ttgctgttgt acgctcagta aaatggaaat taccatttgc taattctgtc
     8281 atagcaacaa attcatttgt aaccagttta gtcgccatga tactaccggc atccactgct
     8341 tctttccatg ggattccgct gatgaaggca agaggggcaa agatgtaacc taggatctct
     8401 tggaaagtga tgccgaatat cccgtggaag atagcattga tcatggcaat caaagcaaca
     8461 aaaccgataa gcatagcagc aaccgtgatc gccacatgga aaccatcaag aatgtattct
     8521 cccaacattt ggaaaaatgt ctgttctttg ttttcttcaa tgatcagctc atcttctttt
     8581 tcttctaata catagggatt tacaattgaa gccagaatat aaccgccaaa taagttcaaa
     8641 acaagggcag tgatcacata tttagaatcg atcagtgcca tataagatcc gacgatcgac
     8701 atagaaactg tagacatagc cgatacactc atggtgaaca aacgtttctc agataggaag
     8761 ggtaattcct tttttatgga aataaaaact tccgattgtc ctaaaatagc agaagctact
     8821 ccattataag attccaattt gcccatcccg ttgattttac ttaatcctag accaacagct
     8881 ttcataaata atggaagaat cttgatatag cgtagaatac cgataatagc agaaataaat
     8941 acaattggca tcaatacgga aagaaagaaa ggtgcacttc ctttatttgc tacaccggca
     9001 aaaacaaagt caacaccttg cccagcaaaa gcaagaagat gatcaaacat ttttgccaat
     9061 acagaaacaa ccgctgtacc aaaagttgta cgtaataata cgaaaccaag aacaagctgt
     9121 aaaacgaaca tcacaattag tggtttcaat ttgattgctt ttttgttact gctgatcaaa
     9181 aaagcaagcc ctaatgtgac aaataaacca ataataccta ataaataact caaataatca
     9241 ctcctattcc aaattccgtt ttattttacc tgttttgcaa ataaaatact aatctttagt
     9301 gaaattttca ttactttttt agagtgaaac gcattcattt tccattagaa aataaagcga
     9361 acaaaataaa aggtttaaaa agtgtaacgt cttagtaaag tctataaaaa aacgaaacta
     9421 tcgattaagc taatagtttc gttttcaaca tttataagta aactttttct attggtaata
     9481 tagattttcg gaagggtaga caggtgcgca ggtaatatcg attcctagtt ttttcaatgt
     9541 ttgttcatca tttttattga ccatcacagt agagtgggct tgtgctccct caagttcagc
     9601 tagtttatca taagcaactt gagcgattgg attcgttact gcactgattg ataaagcgat
     9661 caagacttca ttgatgctta gtgcatccaa agaggaatga agtgtctttg atttcaatcc
     9721 ttgtattgtt tcaagaacta atggtgacaa taaaggaatg tcatctgcga tatgggctaa
     9781 atgttttact gcatttaaga taactgccgc tgacgcatcc atcaatgaac ttgtacgccc
     9841 ggtaatgatg acttcatctg gtaattcgat tgcaatcaca gcagaaggat cttttgtagg
     9901 actttgcgct tgaatcttat ctgcatagtc atgggcaggg actaccacgt ttcggtcttc
     9961 aggacgtaaa ccgacttctt ccataatcag tttgattcga ttaactgctt cttcgtcgat
    10021 caatcctttt ttgaagtcgc attctgtttg gaaataacga cggatgattt cttgtttaga
    10081 ggcttcttgg acaacggtat catccacgat cccaaatcct acacgattca cacccatgtc
    10141 agtaggagat tgataaacag attctttacc agtaattttt tcaataatac gtttgattac
    10201 aggaaatgtc tcaacatccc gattatagtt cacagctact ttattgtagg catcaaagtg
    10261 gaaggaatcg atcatattga catcttttag atcgactgtg gctgcttcat aagcaatgtt
    10321 cagcggatgt ttcaacggaa cattccatac tgggaaagtt tcgaacttgg agtaaccagc
    10381 tgcatgtcct ttttggcttt catgatacag ttggttcaag gaagtagcga gctttccact
    10441 tcctgggcca ggagctgtca ctacaacgat tggtttagtt gtttcaatat actcattttt
    10501 tccaaaacca tcttctgaga cgattttttc tacgttgact ggatagtctt cgatttctga
    10561 gtgtttatag accttgatgt ttcgtctttc tagtttattg ataaagactt tcgtggcagg
    10621 ctgtccacta tagcgagtga taacgacact gttgattaat aagccataac cacgtaattc
    10681 atcgattaac cgcaagatat ccatatcata agtaatccca taatcaccac ggatcttatt
    10741 acgttcaata tctccagcat agacgcaaat cagtatttca gcttgatctt tcaatttttg
    10801 cagcaatttg attttcgcat cttcttcaaa tcctggaagt acacgtttcg catgcttgtc
    10861 gtcaactagc ttcccaccaa attctaaata taatttgtca tagtggttca ctcgttccaa
    10921 aatatacgct gactgctctt caatatattt ttctgaatca aaaccaattt ttttcactag
    10981 gttccctcct taagtactaa agcatttttt catttttttg aggattcgtc aactggaaga
    11041 gcgaaattcc tcaaaaaaac aaagaaaatg gtttaaacca acttgtctag atagttattc
    11101 attggtatga tagtttttga taaggaaggt gctacatgaa aaaaagacag aaaaagaaaa
    11161 acgcatataa acactatatc cgttcgattt ttaccggtta tgaaagaatg cttgaagatc
    11221 ccgaattgga acaattgacg tttacttatc taaacgaaga aacgcagcta actcgtgatg
    11281 agcatcagcg cattcatttc acaacaagag atctgcctag taaataaaaa ataagataaa
    11341 ggtcatcgga ttcgattctg tcctatcaga tggaaagact agttgatcga aaaaaaggct
    11401 gtaccaaatt ttggtgcagc ctgttttatg gagcattgcc cgctcttttt tataacaatt
    11461 ttttacagaa aatggtacta ttgaagtatt aataagtgaa aaacagatga gtaagaggag
    11521 gcaagtagta tgaaagagcc agatacaaat gcaaattatg agccagacga actagaggag
    11581 acgaatgcgg atcaggatac aggacaagaa tcgtcttctt tttacgatat caaagaaaaa
    11641 gcaagcatga atctttccca attattagaa gacttacagg cttttgaaaa agctgtccgc
    11701 gaagaagata tccctgagat ctaccgttta tacaatggcc gattgaatga agaattaaaa
    11761 agaagctcaa atgaaaatca cgagatcgat caattgctga tgcgtaagat ccatgatcgt
    11821 tttttacagg aatttccttt tatggaacaa gtagaaaaca tctcgccaac gatgagctat
    11881 tacaaaatcg gcacttatta ccatgatcgg cccacggtcg gtattgatgc aagtctgcca
    11941 gaaatttttg ttttgcctaa gatcgatgaa gagtgggaga aatattccca accggagtca
    12001 acggactatg acaaaaaaat caatgagcta gacgcaaagg tgatcactgc tcagacagaa
    12061 attgaacgtt tagatgaaca aataaaagaa atcaatcgac aaatgactga tttgaacgac
    12121 aataaaggat tcttgaatcg taaaaaaatc gaggaagaaa ttcaagaatt agagaagaag
    12181 aagcaagtac tatctaatga aaaattaggc tggcttcctt atattgagac gcctgaaaca
    12241 atccagcagc aaaaagaagc attgaagcaa gaagcaagag cagaccagct tcgtgcagcc
    12301 atcgtagaaa aagaacaaag gcaaatcaga cgctatttcg gtagcaaaga gggattcggt
    12361 caagcaatcc atgaattctt gatgaattat cttggcaatg agaatcctaa taatcaatct
    12421 aatgaaggag gttccgagta tgaatgaaca cgatgaagca gaatggacaa aaaatgaaga
    12481 acaaacagaa gatcatgaag aaacgaacac ttcattggaa gaacaaacaa gtaatcctct
    12541 ggatgacaca gaagaaatcc ctatgctaga ggaaacaaaa atacacgaaa aagaaaaagt
    12601 aactccgacg aatacgaata ctgcagaaaa cacccatgct tacgaaataa ctgaaaatcc
    12661 cgggtattta gaaggaaaag agcaagatca tcaagatatc gtcagtttga ttgaaacagc
    12721 ggaagcagaa ttagcggatt tacgtatgag aaaagatttg atcgaatcag attttccaca
    12781 attattagaa tcatatgatc gttttatatt gaatgaagaa ccaatctcca cgatcggaaa
    12841 gacaagcctg atcgaatact tcacgtatga attactgaaa ccgttgaatg atattggtct
    12901 ggaacggtca aattcagctt atgacacatg gaaaacaaga catttttcga tcaattatac
    12961 gttgaatgga caaaacgaat tagtcttttc ctttgtatta ccgaccatcg atcaacgata
    13021 ccaagtggaa tcgatgagtc tcctaaacgt atcaccagag acaatggaaa tcaatgtcca
    13081 agatgaccgg gtgctttctt tgatccgaca ctggtcagtg gatcgagtat tctctgcagg
    13141 gcagatcacg atattcaatc acaaattgaa tcaattatta gcccatgcaa gaacacttgg
    13201 ttttaccgtt aatcagacat tactagacaa tacgaagcca cttcgtttga atcttcagag
    13261 cgaatttgaa ttgacgaatg aagtattgga tgatattttt atcgtggcaa tgaaaaatcc
    13321 atcctacgat atggaaaaga tcaacgaaac tacttatcaa gtacgattag ataaaggaca
    13381 gtctttaacg ataacaacaa acgaaaaaga tcaaacagtc ttgtcgatct catctggaga
    13441 ctacccacga tctgtcattg atttctttat caattacgaa ttcttggtcc cattaatggt
    13501 gagaaagtcc tgattggata agttggatta ccagatgtca ttcagataat tctgttccaa
    13561 aggacttgcg gcagttatcc agaataattg tcacaagtct tttctgtatg tactaaatgg
    13621 acgtgaataa cttgctgatg aaggagggat ttcattagga atgtccacaa ataaaatagc
    13681 acagcttagc ttattgtctg ctgcttgtgt agcaggtagg atcgcttttc aatttattcc
    13741 gaatttccag ccaatgactg ctattttttt atttatagta ctttacttaa gcttaaaaga
    13801 tgcgttaatt gtcatgagtc tttctattgc gatcagcagt ttttattttg gtgttggacc
    13861 atgggtcatc ggccaatgga tcagctatac tgttgttctg agtttgtttt caatcgtctg
    13921 cttgagaaaa acagtgcaaa ggaatctatg gttaaaggga gtctgttttt ttctggcggg
    13981 catggtttac ggaataggta tgaccgtttt tgatacgctg ttatatcgtt tgcctcaacc
    14041 ttggatctat taccttcaag gggtgtcgtt tgatttgatg cacagtatag gcaatgttgt
    14101 gttctttctt gtttttctgc cagtcgtcaa acgtttttac aatcattcag gaggaaaaaa
    14161 tgaaaaaaat aatttatagt ttgcttgcaa tgagtgtcag tatactcatt ttgacaagtt
    14221 gcagtacaac ctatacacaa tcggcttcca accaaaagga ggaagtaaaa acatcgcaaa
    14281 aagcaacaat cacgttacaa gaaaatggtc aaaacttttc ggaaaaagaa gtgttcttca
    14341 aaaaaggcga tgatttactg agtattttga aacgaaattt tgaagtcaaa gaagataacg
    14401 gcttcatcac ttcgcttgaa gggcactcac aggatgagaa aaatcaactc tattggatgt
    14461 ttactgtgga cgggaaaagt gctactactg gtgccaaaga gatcaaattg aatgatggac
    14521 aatcggtcat tttcaatttg tctaaactat aagtttcata aaaaactgtt aaagggcgaa
    14581 tattgccata attcagtgag ttcagttgat gaaagaagga aaataacatg gaaaatttta
    14641 aaaaaagcgg aatgttttcc gtaattgctg ctctaggggc taatattcta gttgctatca
    14701 gcaaatttgt cggctatgca atcagtggca gtgccgccat gctaaacgaa agcatccaca
    14761 gtatcgttga ttgcagcaat cagattttgc tgttattcgg tgataaacaa gccaataaag
    14821 gacagagcga gttgcatcaa tttggtgaag gacgagcaaa atactttttc agtacaatcg
    14881 tcgcaatgat gcttttcttc ggcggtgggg cacttggggt gatggaagct tttgaaaagt
    14941 tgacgcatcc atcccatgaa gtgggtaatc cgatcattgt tatagcaata ctgttatttg
    15001 gtttggtaat cgaaggaagt tcgttacgcg tggcaatgaa agaaatcaaa gaattgaata
    15061 ctgaaaaact ttccacgatg cgctttttgc gcgaaagtcg acacagtgaa atactgatta
    15121 tttttacgga agacttgtgt gctgtaatcg gtttggtctt ggctttagca ggaacaatat
    15181 tgacttatgc aaccgggatt gctgcatttg atgcgatcag cggattgctg atcggtttct
    15241 tgctgatggg agccgccatt ttcctagcaa aagaatttta tagtctgatc atcggtgaaa
    15301 gcgtgacagc aagcgattta tcaaaaatca agttagcttt tgaacgtcct gagatcagtc
    15361 ggctgatcga tataaaaaca gttcatttaa gtccaacgga gattttagtt gcagcaaaag
    15421 cagatatcgt tggtaacaaa gagaatgaag cttactcggt catcaatgat attgaaaaaa
    15481 atatgcgtca gtctcttcct gacaagaaga tgtatatata cattgaaaca gataaatatg
    15541 acccaaatta ttctcgtaaa taaaacgttt attcatgaat tgaagcaaaa atgagaaaaa
    15601 tataatcttg tttttaaata aaacggctac aatttagcga atttatgtta caatgagtcc
    15661 gctgtttatt tgttgtaatt aaaggagata ttttaatgaa caaaaagaaa agtgcgccat
    15721 ggcttcaagc tttgcatgca gtgatgccac tttgtataag ctatatacca gtaggattag
    15781 cttgcggagt attgttgcaa aaagttggat ttgatcctct cttatcagga ctgttatcga
    15841 ttctggtctt ttccggtggc gcgcagtttt tagttgcatc tatgcttacg acgcaagcat
    15901 catttgcaac gacattattg atggttttct ttttagaatt acgctataca ttattaggag
    15961 caagtcttgc cggttttatg aaaaaagaaa agcggacgtt tttggctgtc ttttctcaat
    16021 cactgaatga tgaaaactat gcggtgaact acttaaagtt ttcaactgac ccttcctgga
    16081 ataaaagaaa ggctttatat gtcaattggt tctcaatgac tgcatgggcc ggtagcaata
    16141 tgatcggtaa ttttttcggc tcggtgattc gagtagatgc tgaccttgtc cattttgctt
    16201 tgacggccat gttcattttc atgtttatca tgcagatgaa aagcgcacta ctgatcttta
    16261 caggtttatt ctcaggcgta ttaggggtcg tattcatggt attattccaa aatactttcg
    16321 gattgattgc agcaacagtc attgcttcat ttgccggatt tatgctggag aaggcattca
    16381 aaaaagaaaa agctaagaaa ctaagagacc gcggtaaaga tgtcaatgag ccgctcttca
    16441 acttcccgga acaggaggcc aatcaccatg actaaactat atttgctact tttagttgct
    16501 tgtctgttca tcgtggcata tatcccgcgc ttgttcccga tgctttattt tactcatcga
    16561 aaagtgccat cctggttcag tgactggatg aaatatgtac cggtggcatt atttgcggcg
    16621 ttagctttta aagatgtttt cattactcat gaacatctgg atattgcttg gaatatcaaa
    16681 atagctgcaa tgattctagt agcaggcgtg gcttacaaaa cacgttcgat ggcgttgtca
    16741 gttttgacag gacttgcgtc tgtctttttg ctgtctatgc tttaaactgt tttattttat
    16801 taaaaacaga gattgcgaca taagttgttc aaactgatct atccgaactg catttgcaac
    16861 agtcaattct ggatgcaagt ttattcgtag ggataaaaaa gattagtgtt ggcttatgat
    16921 gcaagctctt ttttatttta ggaattagag agggatttgt ccttctatga ttttttgcta
    16981 taatagggaa gatgtttatt ttatgaaaga atagttgagg gaatttaaat gaataaaaaa
    17041 ttgatgcaag gaacattttg gctaaccttt gctaatcttc tatgtaaagt tttaggggta
    17101 gtctatttga ttccctggct aagtatgatg gggaacaatc aagatggtat gcttgctacg
    17161 acattgtata atgttggcta tcttccttat ggattgttcc tgatgcttgg tacagtcgga
    17221 tttccaaatg cgattgcaaa aaaagtagct gtagccacaa aagaaggaga taacgctgct
    17281 tgtcggctga tttttagaag tacgatcaat atcatgtttg tcatcggtat cgcctctgct
    17341 gcactgatgt atttatttgc tcctttacta gccggaatca gtccgattgc taacgttaat
    17401 aatgggatac tagctatcag aagtttatgc ccatctttga ttgctatccc aattcttagt
    17461 gcaatgagag gatatttcca aggaaaaaat gatctgcggc cttatggaac ttctttgatt
    17521 atcgaacagg ctgtgcgagt gatcgttatc ttggctggta cttattactt gcgcgtattg
    17581 actgacggaa ccatattaga agctgtattg atcagtacag tcgcttcttt cttcggtggg
    17641 ttagctgcga ttatccatat gtatatcgtc ggacgcagaa aagacttctt cgaacttaaa
    17701 gattttttga tttccagccg ttatttcgaa agagaaaacc gatctgcctc tgtctctatc
    17761 attcgggaaa cactaccgtt tatttttgtc ggctcagtca ttactatcgt tcaaatgatc
    17821 gaccaagtaa cgatgaaacc actgcttcac tttttccgtc cagagatcgc agatcagcaa
    17881 ctggaattct tattcagccg tgcttctgtc aatccaaata aattgacatt gatcctgatc
    17941 tccatggtag gaacagtagc tataagcagc ttgccaatat taagcacgat gaagaaaaaa
    18001 gatcggatac aaatcgaaaa aacagtagga gatagttttt cgattgcgtt attgattctt
    18061 cttccttcat tgactggaat gtctttatta gctggaccac tatacacatt gttttttggc
    18121 tatgatcctg aaagtgtcgg atatttccaa atggctttgc tagcttcttt gtttttctct
    18181 ttattcacga tcctatctac gatgcttcaa tctttgaatc accatcgttt tgcgatccga
    18241 ttaactgtag aagcaattat cctgaaagtc atcttccaag taatcggatt aggattagtt
    18301 ggcggatacg ggatgagctt atcaaatggc ttagcattcg gtattgtctt tgttcgcggc
    18361 tatcacttta tgtgcaaaga ataccgaata gcacctttag caaagatcag caattttttc
    18421 ttaaaaactt tccgctccac cctgattatg cttgcagtat gttttgccgt tttctttttg
    18481 ctgaatcagc gattgtcaat ggaatcaaaa agctatgcaa tgatttattg tgtggctgta
    18541 ggaagtattg gcggcttagt atttttattt tctcaattcg gaaaaaatgg gtttcgaatg
    18601 ttgaaaaatg ttaaacatca tagcagaagc aaagaatagg ttaactaatc gttttttgct
    18661 gtcacttgaa aaacggcaac tcgtatgatt acgcttgtcg tttttctatt ttccaatttt
    18721 agtttcatat ctaatcttac ttatgatata gttataaggt ctggaaaaat tctttgaaac
    18781 gaggaaatta aacacatgaa acttgcagta gttacagata gttcggcata tctgccggaa
    18841 cgtatcaaga accatcccga cttattcgtt attccaatcc ctttgattat ggatgggaaa
    18901 atttataacg aaggaatcga tatcgaagcg gatgaatatt atggactttt aaaaaacagt
    18961 aaagattttc ctactacttc acaacctgtc gtaggggaag ttttgggttt atatgaggaa
    19021 ttgaaagaga aagggtatga cacgatttta agtatccatc tttcttcagg gatttctggc
    19081 tttgtcaaca cattatttgc gatgaaagat gacatcagcg gagtaacggt cattccttat
    19141 gactctaaga tcactagtat gccgatggga cacatggtag aagctgcctt agacttaaat
    19201 gatgaaggtc agtcccttga tgaaatactt gcacatatcg atcgaatccg agataatacc
    19261 tacgcttatc tgattgtaga tgatttgaac aatttagtac gaggaggacg tttgacaaat
    19321 ggtgcagcgc tgattggcag cttattaaaa atcaaaccga tcctgacatt ttctgaaggg
    19381 aaaattgttt tattcgaaaa aatccgttca agtaaaaaag catttgctcg agctgaagaa
    19441 attattggta gaagaaataa cgaaatacaa ccgccagtca aactttacgt gatacatgca
    19501 aataatttag aagtagcgat tgcggagaaa gagaaattgc agaaaaaata tccaaatgca
    19561 gaaatcgaaa ttggtcattt cggaccagtc attggtacac atttaggtga aaaagccatc
    19621 ggcattgcca tttcagcgca ataattcctt ttattcaaaa aacataccaa aaatattcca
    19681 acatcatctt ggactttcta catgttacta atgagcgtgt aaaagaacga gatggtgttt
    19741 ttatttctat caatgaagct ctttttcttt gcaatcctca cttcaatcct ctataatgtc
    19801 gcatatagtt tagtaaatga gtaaactact aatttactta tttactaaaa ataaaaggat
    19861 gaggagttaa aataatggaa ataccaacaa atttaaaaca taaaccagta atcgttgtag
    19921 aagactatga taaagttgat ggaagaaatg cattaaatac ggatgcaaaa gggctttcac
    19981 tgggccttgc tcagtggaat gatcgtggaa aagtcgatat ctcaggcaag gtatggcgac
    20041 atactggaga aaaatggtca cgacagtcgg aagaattacc aatcacacgt attttagatt
    20101 tagcgatctt agctgcacaa ggaagtttat acttccaaga tgcttatcgt tatgaaaaat
    20161 tttatgatcc agaaaatcca gtcgtagaca tcattggatt acaagggaac cgaatgacgg
    20221 tagaagtaga taccaaaaat ccaaagatcg atgaagacat cattttgtat tacgatacac
    20281 tacaaaaaga cggagaactg attggggaac gattccgtat tttaaaacgt cttttagatg
    20341 agctaggata ttaatagaca gagatgatca aaaggggaag agaagcatgg agaatttctc
    20401 actaacagaa aaccgaacct tttcacggct agaaaagcaa atgatctgga agaaaccgaa
    20461 aagccatcgg acaagtgcag aagaatggcg gatcgcctcc gaaatcaaag ggaattggca
    20521 agatcctgaa atgaaaatca gtaacgtact tttggaagga gatgccgggt cggggaagac
    20581 acaattagcc aaagcactat ctgctgatct gcaacttccc tatacaaaag tgacctgttt
    20641 tgcagatatg gacaaatcag atgttttcgg agccttatta cctgtagtag attctaataa
    20701 ggaacaagac caagaattac tagaagctat ttaccaaaca gatacacttg aagctgtact
    20761 gacactgatc cagaaccatt acggcattga tcctttttct gctaaagaaa aattagcaga
    20821 gctagttcaa aggatcgaag ctgattatcc agatgcaatc cattacagct attacccttc
    20881 tgaaatcgtt cgagcgattg aaaaaggata tttattagag attcaagagc cgactgtcat
    20941 ccgagatgcc tctgttcttg tggcacttaa ctcagcgctc gaaccaaatg ggatgttgaa
    21001 tttaccgaca ggaatcattc gtcgccatcc agattgcgtc attgtgatca ctacaaatcg
    21061 aaattaccaa ggaaatcgcc cattaaatga atctttaaga gatcggatgc aacatgcgga
    21121 aaaaatggat cttcctgcct tagaagtcat ggcagaacgt gcgatggcga aaactatgat
    21181 ccaaaaacaa gaacttctct taaagatggc agagattatc cgtttgttgg atgaaactgc
    21241 aaaagctaac gccatcaaag gtgtagcagg aatgcgttct tatttttatt gggcaaatac
    21301 ctataagcaa ggacaagatt tactccagtc gatttatcca aaagttttgt ataaattatc
    21361 cactgattca gatgaattgg caattcttga acaagctttg tctgacagtg gattgctact
    21421 ggaacttcag gacatattga gaaaagaaaa atggggtcat cctcaatctg agcatacaaa
    21481 aggaagaacc attagcgccg aagaagcaaa tgaacgaaac atcactgaaa atgctgaaga
    21541 aattctgcgc ttagatgaaa agaaatcgtt gtctgctgaa gaaccaaatg agggagaaga
    21601 gaaacaagaa gaaacaatag aaaaaaacga gattcctcct caaatgcaaa aagaacgcag
    21661 tagcgatatc gaaggaaaaa atcaaaaaga acaacaaaca ggagatgatt ctcaagaaat
    21721 gtctgcttct gacatggaag cacatgataa ggaaatcaag aagcaactga ataaagaagc
    21781 gcgacaaatc atgaaggaaa cgattcatga aaaagaaggt ctcatcgttc accgaccagc
    21841 tttccaagga aaaacgccgg aagcacgtga gctacacatg caagtaatgc caatagtaga
    21901 atcacttagc cggcaaattt tagagttact ggataatgaa caaacagaga cgtaccaaaa
    21961 aggaaaatac gaaggtcagc ggttcaatgc aagcaaagta gcatatggtg atttgagaaa
    22021 ttttgacaaa aagaacccgc cacatgagca accttcttta gcggtagctg tacggatcga
    22081 tgaatctggg tcaatgatac gggatgatcg gatcgaagcg gcaaaaaaag cagcgattgc
    22141 cattgccgaa ttttctaaga aagtcgatat cccattgttg atttacggag atacagcaga
    22201 ccgttcgtca agagaaaaga cttctttgtt ttcttataaa gaatttgaag acggatttca
    22261 atggttaaat gaaaaattag tcactatgaa accaaggcaa aacaatcgtg atggtgctgc
    22321 acttcgttta gcagcagaaa aattaaacag acaatccgca acaaccaaac tgttgcttaa
    22381 tattagtgac ggtcagccaa aagcgttgcc ggattataca ggcaaaaaag caaaagaaga
    22441 tatccagtct gttttgaaag aatatgaacg tcaaggaatc ctatttgtct ccgcagctat
    22501 tggccaagac aaagaagaaa tcaagtcgat ctacggtgaa agtcgttttg tagatattac
    22561 tgacttgaat gaatttccca agcaaatgat tcaactgatt gcccgctatc tatgataaaa
    22621 taaagctcaa cgaacgcact tagcagattc gttgagcttt tcgtttctag ttaccggtaa
    22681 caaggatact tctaaaatag aatcggttat aatcaagagg gaccgttaaa ggagtgaaac
    22741 gatatgatac aagaacaaaa atggtggaaa gaagcagtag gctatcaaat ctatcctgct
    22801 agtttcaaag atagcaatgg cgatggtcaa ggagacatca atgggatccg tgaaaaactt
    22861 aattatttga aagatcttgg tatcggcttt atctggatca atccgatcta tcaatctcca
    22921 tttgtcgata atggttatga cgtaagtgac tatcaagata tagaagagag attcggaacg
    22981 atggaagatt ttgatttatt gctaaaagaa gcacatgaat tagggatcaa agtgattatg
    23041 gatcttgtca tcaaccatag ttcggatcag catcaatggt ttgaagaatc gaaaaaatct
    23101 aaagaaagcc cgtatcgcga ttattacatt tgggtcgacg gggtagatgg caaagaaccg
    23161 aataattgga catcgatttt cggcggttct gcttgggagt attctcatga aactggacag
    23221 tattatctgc atgtttttgc aaaagaacaa ccagacttaa attgggaaag cgagaaattg
    23281 aaagaagaac tattcaatat gatccgatgg tggttggata aagggataga tggatttcgt
    23341 ttagatgcga tttctcatgt aaaaaaagat gaatattcag taaaagccac agagaaccct
    23401 ttctcaccct ttcaaaatgt ctcaggaatc gaagaacatt tgaccgattt gaaacatgtt
    23461 tttgaagagt acgatatcat gactgtcgga gaagcaagtg gtgtcactgc agaagaagga
    23521 ccacaatggg tagggaaaga cggttatttc gatatgatat tcgaatttga tcatatccat
    23581 atctggcagc aagaaaaaga agggcaatta gatgtcctta aattgaaact tgcattaagc
    23641 gcttggcaaa cttcattgga tggtatcgga tggaacgctt tatatatgga aaaccatgat
    23701 gttcctcgtg ccgtttcagt gtttggagat actcgtcccg atttttgggc tatgtctgca
    23761 aaagcaatcg ctatgatgta tttctttttg caaggtacgc catttattta tcaaggacag
    23821 gaaatcggaa tgacgaatat gccctttgaa tcgatcgatc aagttgatgc agtagattcg
    23881 aaaagacttt ataaaaggct gcttgcggaa ggtaagacaa gagaagaagc attggatatc
    23941 attcgtgaaa caacgagaga caacagcagg acacccatgc aatggacttc ggaacaatat
    24001 gctggttttt ctacacatga accttggctg atcactaatc caaataccaa aacgatcaat
    24061 gtagaacaac aagaatatga accagagtcg gtcctgcaat tctataagaa catgatccgc
    24121 attagacaaa ccaataaagg tctgatatac ggttcttata aagaatatct tcatgaacat
    24181 ccacaacttt atgtctatga aagatattta gaagatgaac aatatcttat tatggtaaac
    24241 ttaacagaat cacttgctga ctatgaactt ccaaaagaag cagatcagtc ctggacatta
    24301 ctcttgtcaa attcctctag cggagaattt gaagctaaag gcatactagc accttatgaa
    24361 gctcggttat ataaaacaaa caagtaaaac ttcctcgtta aagttcatta gttggacttt
    24421 gcgaggaagt ttttcagatt gctctgtcaa ttactcattt caataattcg ctctaggacc
    24481 accgatatat ttaggacgag agcgaagggc tgtcacgtga gcttgtccaa gggttttcaa
    24541 acgaggtctg acaggtactt ggacatgttt gatatgcata ccgattgatg tgtcaccgat
    24601 atcgataccg gcttgtccaa ctacgtgttc cacttcgaca ggatcattga actggtcaaa
    24661 agcagcaaca gaacaagcac ctccagcatg tagtgcagga acaacagaga caatttcgaa
    24721 attttttgct tctgccactg ttcgttctac tactaatgct cggttcaaat gttcacaacc
    24781 ttggacagct agcgcaattt ctttaggatc caacaactct tttaacgtac ggatgatcca
    24841 ttgaccaact tccgcactgg aattttttcc aatatgtcct ccaacgactt cgcttgtgct
    24901 gcatcctaaa acaaaaagat cacctttttt caagtgtgcc tcagccaata tgtcattaac
    24961 tatttcagtc aattggtctt ttaattcttt ttctgacaac ttcattttct aaaaccttct
    25021 ttttcaatta atattgcttc cgtgcttgaa tacgttgaag acttgcagtt ataggaaagg
    25081 caagtcccat tccaatcaga ttttggaaga tgtttcctgg tatagaagcc actccagccg
    25141 gccaatcata taaaatccaa ccggcgatac catagccgat gatcataact aagcttccta
    25201 ataaaagccc taatccccac atacgagtag aagatttgct gaaagaacca gcaacatatc
    25261 cttgtaaccc gtggatcact aatgaaaaaa ctgcccactg cggatatcct gaaatcaaat
    25321 cgatcaatag accactggaa gcaccaacaa ctaatccacc cgttggacca taaagaaaag
    25381 cagcggtata gatcccggcc tcgcataatg tcacaaatcc attcgtagcc ggtacaggga
    25441 tcaaaattgt taatgaaagg gcaactgtca acgcaatcag catagcatag atcgttaatt
    25501 gcttagtttt catttctcat tcctcctttt tgaataccgc cagaccagac acttccggta
    25561 tgatcgttca tcagcacgcc attagaaata caatgggcaa caaatttttt actttctgct
    25621 actgctttgg taaaatctgc ccctaacaca agattcgctg ttattgcaga agaaaaggca
    25681 caaccggccc cgtttactgt tgtagtgtcg atttttttat gagaaaatac ctcataatga
    25741 tcaattgaat agagaagatc cacagcttca tctccagcca tccttgtgcc gcctttgatc
    25801 acaatgtttt ttgttcctga agcagtgagt tctttagcga gatggatcat ttctgttaac
    25861 gaagagggaa gagcttgacc agaaagtaaa gcagcttctg ccaaattagg cgtaatcatc
    25921 gtaacaagtg gaaaaagttc ttcgatgagc tgcgaaatat acaattgatt gatcacagct
    25981 tcagtttctt tgaatgccaa aacagggtct aaaatgattg ggatattttg ttttgttttt
    26041 atgaagtctc gaacaaccat gattgcttca cttgaattca ataacccaat tttgatacca
    26101 tctaatgaag tcattttatc aatcgaaact aactgtcgtt taagcaattc caaagatata
    26161 gattccagca taaatcgctc attaatatct agtgccccta tagaagtcaa ggcgcttagt
    26221 ccgaatagac caaaattctc aaaagttttt aaatctgttt ggattcctcc accagcaaat
    26281 ggatcagaac cgccaattgt caagattctt ttcataacat tttcctccat gaataagagt
    26341 ataacgaaat tttcaaaaaa gaaaaacagc caattggcat atttttatct catccaattt
    26401 aaaaaaacaa aaagaaaaga aagaccaaaa tggatctttc tttccttaaa tagttaggac
    26461 ttttgtgatt ttaattcttt ctcgatttga agccgttgca ctttcattgt agctgttcgc
    26521 gggattgcat caaaatcacg gatgataggg acgttcaagc gaggaagttc atggacttgc
    26581 tcccaccatc gattccaatc catttcttta tcaggagcta aagcaataat cggttgtgga
    26641 gaatgatttt tatcccttac aatcactact tctgccaaaa aatctaacga atctaataaa
    26701 aagtcttcaa tagctaatgt actattgatc gtttcgatca aatccacttg ccgatctttc
    26761 aaaaataggt ggccatgctt atccataaaa ccgtaatctc cgctatccca ccaatcgcca
    26821 taaacatttt cctggaaccg tgcatcttcc ttgtaataag tcaaagcccg tcctttagac
    26881 agtaattgta tatgaccatc tgtgttttca ggtaggacat tcccaactga atctgtaatc
    26941 cgagcgcttg tcatgtcttc cagtccaacg cccatatctc ttgcatcact tgttttcaaa
    27001 ctctctagtg tatgagcctt aagaatcatt ggtccgcatt cactttggcc atatacttga
    27061 agaaataccg catcctgtgc catcgacgtt cgcaaaaatg tcttcattgt ctggttgttt
    27121 atagcatcaa aagtggagtg atagtatcgg atacccgaaa aagcttctgg atgctcctta
    27181 gcagtaaaac gccactgaac aaagttattc ggatgggtct ctagtgcaat tggccggtga
    27241 gcttccaaca tatgaacaac ttttgaactt tgtgcgtttg ctaaaggcat cataggaaat
    27301 cccatggaca tcaaagaaga gataccaata ttgaatctgg aatgcactgg agagatgtga
    27361 aaagcgatta atttcttttc cgaaattttt gtgaaaactg ttttttgcca tttcgtccgc
    27421 catcccatag aatgagcaga atgacaaata agtttaggga taccagttgt accagaagta
    27481 tgggtcatat aagaaatttc atttttgtct aaaaatgatt gagaaacaga caaagcaggt
    27541 tgttccatta aataaagaat aggaa
//
LOCUS       DAZBTF010000034        25366 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000034 DAZBTF010000000
VERSION     DAZBTF010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.034"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            21..1658
                     /locus_tag="ACHGNS_001523"
     CDS             21..1658
                     /locus_tag="ACHGNS_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937474.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(1803..2543)
                     /gene="truA"
                     /locus_tag="ACHGNS_001524"
     CDS             complement(1803..2543)
                     /gene="truA"
                     /locus_tag="ACHGNS_001524"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFN6937475.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(2734..3369)
                     /locus_tag="ACHGNS_001525"
     CDS             complement(2734..3369)
                     /locus_tag="ACHGNS_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFN6937476.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3516..4736)
                     /gene="sstT"
                     /locus_tag="ACHGNS_001526"
     CDS             complement(3516..4736)
                     /gene="sstT"
                     /locus_tag="ACHGNS_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFN6937477.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            4955..5953
                     /locus_tag="ACHGNS_001527"
     CDS             4955..5953
                     /locus_tag="ACHGNS_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937478.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            6149..7984
                     /locus_tag="ACHGNS_001528"
     CDS             6149..7984
                     /locus_tag="ACHGNS_001528"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFN6937479.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(8044..8760)
                     /locus_tag="ACHGNS_001529"
     CDS             complement(8044..8760)
                     /locus_tag="ACHGNS_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFN6937480.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(8987..9529)
                     /locus_tag="ACHGNS_001530"
     CDS             complement(8987..9529)
                     /locus_tag="ACHGNS_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFN6937481.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(9534..9761)
                     /locus_tag="ACHGNS_001531"
     CDS             complement(9534..9761)
                     /locus_tag="ACHGNS_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFN6937482.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(9840..11720)
                     /locus_tag="ACHGNS_001532"
     CDS             complement(9840..11720)
                     /locus_tag="ACHGNS_001532"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFN6937483.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(11909..12523)
                     /locus_tag="ACHGNS_001533"
     CDS             complement(11909..12523)
                     /locus_tag="ACHGNS_001533"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6937484.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            12665..13225
                     /locus_tag="ACHGNS_001534"
     CDS             12665..13225
                     /locus_tag="ACHGNS_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN6937485.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(13530..13844)
                     /locus_tag="ACHGNS_001535"
                     /pseudo
     CDS             complement(13530..13844)
                     /locus_tag="ACHGNS_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(14098..15733)
                     /locus_tag="ACHGNS_001536"
                     /pseudo
     CDS             complement(14098..15733)
                     /locus_tag="ACHGNS_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16172..17041)
                     /gene="scrK"
                     /locus_tag="ACHGNS_001537"
     CDS             complement(16172..17041)
                     /gene="scrK"
                     /locus_tag="ACHGNS_001537"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFN6937486.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(17061..18182)
                     /locus_tag="ACHGNS_001538"
     CDS             complement(17061..18182)
                     /locus_tag="ACHGNS_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFN6937487.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(18184..18579)
                     /locus_tag="ACHGNS_001539"
     CDS             complement(18184..18579)
                     /locus_tag="ACHGNS_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN6937488.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(18598..19395)
                     /locus_tag="ACHGNS_001540"
     CDS             complement(18598..19395)
                     /locus_tag="ACHGNS_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN6937489.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(19397..20185)
                     /locus_tag="ACHGNS_001541"
     CDS             complement(19397..20185)
                     /locus_tag="ACHGNS_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFN6937490.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(20199..20675)
                     /locus_tag="ACHGNS_001542"
     CDS             complement(20199..20675)
                     /locus_tag="ACHGNS_001542"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN6937491.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(20747..23404)
                     /locus_tag="ACHGNS_001543"
     CDS             complement(20747..23404)
                     /locus_tag="ACHGNS_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFN6937492.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKR
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNIRKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            complement(23468..23827)
                     /locus_tag="ACHGNS_001544"
                     /pseudo
     CDS             complement(23468..23827)
                     /locus_tag="ACHGNS_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
     gene            complement(24005..24325)
                     /locus_tag="ACHGNS_001545"
                     /pseudo
     CDS             complement(24005..24325)
                     /locus_tag="ACHGNS_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(24441..24962)
                     /locus_tag="ACHGNS_001546"
     CDS             complement(24441..24962)
                     /locus_tag="ACHGNS_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937493.1"
                     /translation="MDKPFLDSVREYLSNPKSTLFPHEDYKIIMNYYLPIGAPQNSKI
                     KIPNDIVNDGDTHNVIKGFLPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGL
                     DLKNLLCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLM
                     ELKKYSNTQTKFS"
BASE COUNT         8001 a         4958 c         3837 g         8570 t
ORIGIN      
        1 cttttaaata ggagaaaaaa atgaaaaaaa acaaaaacca aataatagat atcattttta
       61 tgttatttct aagttttgct tacgtcatcc ctctattcat gtctaaggga atctatcata
      121 gtgtcaatca ggatacttac tttcatttat ctcgaatcat aggactagat aatgtatgga
      181 gttctccagt aaacttcaat aacttcgatc atcatggaac aatgatgaat attttttatc
      241 catggcttac tctttatcca gcttttttat tttataaaat gatgggaaat cttgttttaa
      301 gttataatat ctactatttt tttatcactt ttttaacaat ggtcgtttct tatttttcta
      361 tgaaacaaat aaaaaacaat cgttatattt cgctgttatt ttcgattatt tatacatttt
      421 ccgcctatcg tgccattgac atattccatc gtgcttcatt gggagaagct gtcgctttaa
      481 catttttgcc tttgatacta atggggtgtt acgaaattta tattagagat taccaaaaat
      541 ggtactggtt gtctattggc atgacgctag tagtgtatac tcatttactt tctgtcgcta
      601 tggtttctgt atttattgga ggaacgttgt ttctttcttt ttatttttgg gaccaaaaaa
      661 tcgctagact cttaagtctt ttaaaagcta ccgtcctcac ctttttttta agtgcgggat
      721 tcttaattcc ttttattcaa caatctagag cacaagaatt gaaagtccca ctgggaaaag
      781 aattaagtgg tatggctcct agtgatatgc tgacacatat tttaaataat aactataaca
      841 actatactat tggattattc ttatttctcg gattgattgg tgcttttatt tttataaaaa
      901 aattaactgc tgatgattta tttattttct ttcttggtgt atttgttctt ttttgttcaa
      961 ccaatttatt tccttggcaa ctttttaacc atacaccagt taagagtcta caatttgtct
     1021 ggcggttaaa tggtttttct tctttattta tagcctatac gatgagtata gtgatctatt
     1081 atattttttc tactaaaaat aattcatgga aaataatggt ctttacgttt cttgcactaa
     1141 tattgcatct ctcaggagta agcaattcta tccatgctaa caaagattca ttaacactta
     1201 tagcacctga agacgctgtt gctatagctg aaaattacaa tcatactgat tatgcaaaca
     1261 aagagtcaat ctatcatcct gatatgatta ataatgatca atatttacta ggtaaccagg
     1321 aaataaatcc aacaactcat tttatgagta acaaacttac aattgaatta gataattcga
     1381 ataataaaaa tactgtcttg acgacaccta tttatcgtta taaagggcaa gtggcttcta
     1441 ttaatggcaa actagtacaa acaaaattat ctaagtttgg cacaactgaa ttaactattc
     1501 ctccaggtat aaataaagtc gttattactt atcagtacac aaaactagca attgcttcaa
     1561 gatatttatc tatcgttact ttgattttat ttttacttta tcgatttact ttttctaaac
     1621 aaaaacaacc gagaagagaa gtacaacatt ctcactagat ttttatttaa gaaagacttt
     1681 ttagctgtac acataaacag gctaaaaagt ctttttttat atttctttaa tttgcttcat
     1741 acattattta acatcgatat ttatactgtg aaacatgcgt ttcatccatt atcaaagtat
     1801 ttttatcgat aataaacttc atccaaaaac agaccttgtg cagggacggt ttctcctgca
     1861 tgttgccgca ctttgctttc aaatatttca tcgattgttt ctatcggcat ggcacctgtg
     1921 ccgatttcta gtaatgtccc catgataatc cgcaccattt tatataagaa tccgtctccg
     1981 acaaaagtaa aatgaagcat ttctcccttt cgttcgatag ataattcatc aatcgtacgc
     2041 actgtggatt tttttgattt tttcaatgca gaaaaaccaa tgaaatcatg tgtacccact
     2101 aatttttcgc aagctgcatt cagcttgtcc atatccaact cttgagggaa ataaaagctg
     2161 tggtttcgct caaatgctga aggaatcacg ttgttccaaa cataatagct gtattttttg
     2221 cccaccgcat tatatcttga atggaagcgc tcaggcattt cttcgattct tttgatgaca
     2281 atatcccgcg gaaggtaacg attcataaag tcgatcattt ctgccagtcc aatttctgtg
     2341 tttgttttaa aatttgctac ttgtcctcgc gcatgggtgc cagcatctgt tcttcctgat
     2401 ccaataattt ctatctcttc atttgtcatc tgatgcaata ctttttcgat ttttccttga
     2461 atggttttat cgtcatctcc aagtctttgc catcctgaat atctttttcc gtcatattcg
     2521 attgtcaact tgatgtttct catattttag tggctcctct gtcttccatt tggtcttctc
     2581 tctacctttt taagattgta gcacaaaata tagctccttg cttctctaag acagaagcta
     2641 tacaagaaaa ctgtggaaat atatatttca gaaaaacaaa aaacagccac cggatgaagt
     2701 atccgctgac tgttcaattt gacggctcaa gttttactgt ttgagatgca agatactcaa
     2761 ccaggcaatg ggcacataat taaagaaaaa actgattctt tggcaaaaac ctattttatt
     2821 cagtaatggc caagaattta tagaaggaat tcttgccagt ccataaatcg ccgcaaaaag
     2881 caaactgatc acagacagtc caaggtataa gtggcttacg tccatttgcc cattttttcg
     2941 atagatcaag aaaagaagaa ctggaaacaa taaaaaacca ctgtaaccta aacctgatcc
     3001 gatattatgg acccatgtcg agacattcca cgaagattca tgggtattga tactgaacaa
     3061 acctgtaaag atacaatcac ctataccata gaacgcgatg gctgctgtaa ctagggtagc
     3121 taacacacga gatgtactaa caaatgcagc atacaatgca ggcaatgaca ggatgaaaaa
     3181 gatccctgaa accacagacc aaataagaaa cgcttttctt acaggactgg aaacctctcc
     3241 aaatacactg atcacgctct tcatttgatc taattcagga taatacaatc ctaatacgta
     3301 cggagtcaaa aaatcactca gcacgcctaa taataaaaag aaaaaaccaa atttacgtaa
     3361 aaaatccatg atacgcctcc aataacaatt acctatgctt ttaattgtta ttataaacga
     3421 ctttatcttg atttaacaga aacctctatt tacacgaaca tctatataac tcattaaaaa
     3481 ctcgaatcat tttaaaaata ctaaatatct aacgttcaac gttcacttaa tccatctaaa
     3541 caatctttca atttgatttt ttccccactt cttttacgcg cgcctagctc tcctattgca
     3601 gtaaacaaca gatcacttga cgaatttaag gcagtttcca ttgaatcctg gataacaccg
     3661 ataataaatc caataccgac gatttgcatc gcgacatcat tagagatacc aaacaaacta
     3721 catgctaatg gaatcaataa cagtgaaccg cccgcaacac ctgaagctcc acaagcagct
     3781 aaagcagaca aaatacataa aaggactttc aaaacaaaag gtgcttcaat ccctaaagaa
     3841 tgtacagcag tcaacgtcat caaggaaatg gtaatcgcgg caccgcccat attgatcgta
     3901 gcacctaacg ggatcgatat ggaataagac tcttttgtca atttcaatct ttcagccaac
     3961 atcatgttta tcgggatatt tgcggcagaa cttcgggtaa agaaagcggg aagtgcgctt
     4021 tctttcaaac aaaagaaaat caatggatag ggattttctt taataaacaa aaacgtgagg
     4081 aatgggtaca caactaatgc gacaaacagc atcgtaccga tcaaaacaag taaaagctgc
     4141 agatactctg ccaatccagc gattccagtc gtagcaattg attgataaac caatcctaaa
     4201 atccctatcg gagcaaatgc aataatcact tgtacgactt gtgacaacgc tatagatatc
     4261 tgatccacca actcttttgt cgattctgaa cgtgctcgaa aagctagtcc caacaataca
     4321 gcccagaata agacgccaag gtaattcgca tcaataagag attgaacagg attttgtgtg
     4381 acattcgtca acatagtaga taaaacactt tccaaacttt gcggtgcttt ttcagatact
     4441 gtttcttgta aagtaatagg aacagtaaaa attgaactta caataactgc tacaagtgca
     4501 gaaagcaatg ttcccactaa atataagatc aaaataggct tcacgaatgt cttattccca
     4561 attttatgct tggcaattga cgccatcgtc aagaaaaaaa ccagaatagg tgcgattgcc
     4621 ttcaagcaac tgacaaacaa cgttcccaat attgaaataa atgtccatgt ggggaaaaag
     4681 ataccaaaca atattccgac taaaatacca acaacaatac gttgaactaa cgacatatcc
     4741 aaaaacttct ctaacaaatg accacccttt cacaatttta acaatcattg tatcatttta
     4801 tgcaaagaaa ctaaaggtat aattttctaa aaattctaac aatacaaaca accatttctt
     4861 gctaattttg tactttttct gtctacacgt tgttttttga gatattctaa gttaggatgt
     4921 tattaatggg caacttatca ggaaacgttt tcttatgttt ttgtccaatt tttttgtgag
     4981 gtgtttattc ttgaaaaaaa taaagaagga gagttatggc ttcctgacaa tctttgtcgc
     5041 ttgtataatg atggttttac agcgtatatt gatttcagca gtcactattc ctacatatgc
     5101 aacaaaccct ggtcgattct acatctatac atttttacaa acttccgttg ttattggtgc
     5161 aaatttgatc ccactttaca tgggttacca atatcaaaaa atcgcccgtt taaaagtttt
     5221 acattattta agtcgttttg cctttatatt cctaatagcc acactagttt gcaatatctt
     5281 tttttacgta caagctggca gcttgaatat tcgtgattat tggcttatcc taacacctat
     5341 cagtcagaat tactttactt atgctgtttc ttgtactttg cttttttgta gtattcctta
     5401 tacagttgga tttttaaata aattatcgaa aacaacaatt aaatatctac tattattttt
     5461 aacaattgga ttagttggct gctctactct atttaataaa gacccgtttg cattacagaa
     5521 tggacagagt attttatgga ttttttattt atttttaatg ggttatggtt tgaaagaatg
     5581 gaactggaaa gaaaaggtcc gctataagct tcctcaagta tttatcagct tgattgtttt
     5641 attcggtttg attattttga tgacacaaat ttcactcatc atcagaggaa atacagatac
     5701 agcgctgagg ttttctaaac catattctct cttcgcaatg tattatactt tctcttcgtt
     5761 tttattttta gaaatgctat ctgataaatt aggtataaaa ggaagaggaa attttcttgc
     5821 tgtatttttg attgtcactc aagttatatg taatttccct ttagtttctt atcgcgtatc
     5881 tacatttatg aaacgtgaat ttccaaactc agggaaggca tggctgataa atatcgggca
     5941 gttttttatt tgatatttat tagcagtgtc cctcctagtt atctttttat tttatataca
     6001 aaaaatccgt ttcattcatc gtttcatcga atacttcgct tttgattcga ttgaacaatt
     6061 aactcagcgt tggaaaaaaa gaattcattg gctttttgtt cataaaagtt attttttagt
     6121 tgcagcattt ttttactgct ttaccttcgt gcagattttc gttttagaac caaaagaagg
     6181 ttggaaagaa actatccagg ttgccttaaa aatatttact cagcgacaag cacccatgat
     6241 cctgacaatc atcattatta tgggattttt cttcttacta ttgttgattt ccaatcgttt
     6301 ttggtacgcc ttgactgcta ccttgatcat taacttactg ctcactattt cgacagtaat
     6361 caaaatggaa atgcgggaag agcctgtctt tccttcagat ttaaaaatgc tgaccggatt
     6421 gtcagaacta ttatcgatgg ttagcccagt cttattaatc gtgggcggat tgatccttct
     6481 cctattattg attactagta ttattgtaca acgtagacta caaaaacagt attccttgaa
     6541 gattcattgg aaacgtcgat ttatcagtat cgctgtatta ttaggatgtt tttcgggtgt
     6601 ctttttcatt aatcacaaaa attcgccttc tttcttgctg tttaatttat ttaaagtaaa
     6661 caagactttc tttaaccagc aaggcgccgt aaaagataac ggaccagtca ttcaattcct
     6721 aaacaatatc gacattaaaa tcatggaaga acctgctggc tattcgaaag aaaaaattaa
     6781 tggaataatg aaaaagtaca acacaaaggc agatgaaatc aaccaaacaa gaagtgactg
     6841 gttaaacaat cagacgatcg ttctcaattt aagtgaaagc ttcagtgatc ctgcacgtgt
     6901 ccctaatctg acagtaccta cagaccctat tccttacatc aaaagcatta taacagaagg
     6961 aacaggagga ttgatgttat ccgtcggtta tggtggcgga acagctaaca tggaatggga
     7021 aggactgaca ggcttgagta tcagtaacct gtctgcttcg ttagttacac cttataccca
     7081 gctagtggag cgtcagaaaa taagtccaaa tattaccgat ctattcgatg aaaaaatagc
     7141 catccatcct tttacagctg ctctttatcg cagaaaacaa gtgtttgata aatttggttt
     7201 tgacaggttc tattatttag acagtccgga taaattaact tatacggata aaatagaaca
     7261 aagcccacgc atatcggatg attcagcata caaagaaacc ttgaaagctt tacaaagcaa
     7321 tacggaaacg tcacaattca tccaattatc cactatgcaa aaccatatgc cttatgatga
     7381 ttactatagt gaattgaact acactgctga aggaactgct gtcattccta atagaagaaa
     7441 agaattacaa acttatatgc aaggccttca ctataccgat acagcagtaa aaaaattcat
     7501 ccaagaaatc gacaaaatcg ataaaccgat tacctttgtt ttttatggtg accatttacc
     7561 cgcaatctat agtgggaatg attcgaaaaa atatggatta gaacagcacc agacagatta
     7621 tttcatttat aacaacaagt atagtcgtga acaagcagct acgttaaata aagaagttgt
     7681 tgctcctaat aactttggtg ctatggctct tgagcatgcg aatatcaaag taactcctta
     7741 ctatgcgttg ttgacagaag tatctaatca tctacctgca ttaacaattg atcctacaga
     7801 aagcttgtct aatcgtttta atggaaaaca ggtctttgtt acagaggata gtcaaattat
     7861 ccaagaaaaa aatttaactg agaaacaaaa agaaattcta gaagattatc gtctgataca
     7921 atatgatctt gttgcaggta accagtacag tgcaacatgg gcgacacaaa aaataaaaga
     7981 ctaacaaata aactgagcaa ggggttgtga taaaagacat gtcacaaccc cttgtttatc
     8041 tcattaattt gcttgtccat aaacgatttg attaaagcca tttttcaatt cattattcgc
     8101 tgtctcgttg aagtaaagat aattatcggg attcccctga ttttgttgtg tcaccaatac
     8161 ttttggctga ttttgctgaa acccgaatag atagacgtgc tttttcagat atggttctgt
     8221 ctctgcatcg gaatagacgg ctgtgatttg atagtctgct tgacctgtcc cactttctga
     8281 ccattctgca gaaacaggca ctccaccaat agccatcttc cactccccgt tcaaaatagc
     8341 ttgagggact ttaagaccat ataagtccac actcatttga ttcgtataac ttttgtattg
     8401 ttgtcccatt gtttggcccc atttggtcat aaatgcctct aattgtgcgg atttgtctgc
     8461 attccacagt gtttgttcaa cttgttcgct gctttctgaa gaggaagatg aagcctctac
     8521 agaagaactt gaagtagatg tttgagttgt ttcagtcgtt ttggaagact ctgacgaagt
     8581 agaggtatct gctgtttctg tacttgctgc agttgacttg ctctctgtgc tagttgttgt
     8641 agtagttgca actgttgctt tcgctgtttc ttttgttgat tcttttttcg attcattccc
     8701 gttggtacaa ccagctaaca aaaaagctag accaattgtt actaaaatcc tttttttcaa
     8761 ttaaaaaact cctttcgatt actcccactt ctttattcta tcaaataagc aatgtgattt
     8821 tattgatttt tcgtattttc tcatcaatct ttcaaatctt ctaaaaactg agaagcaccc
     8881 gctgatgttg tctgtaatac atcagcgggt gcttctttta ttttaatctt cgtgattttc
     8941 caatttacct cacgctttat atttgtattt gaagaaacat agacttttag tcatcatcgt
     9001 cttcctcatc caaatcttgt cggacagttt ttccttggag tctttgtact tggtagatga
     9061 tttctttatt gaaacttaaa agttcaacaa gcaattgttg caagccatat ttttcttctt
     9121 tttcagctaa tttgattgcc cgtgtgataa agacattttg gaaatcaaaa tcttttgcaa
     9181 agttttccaa cattgttgag gctggtaaat atttgttttg accatgttcg taaatttttc
     9241 cataacgcat gacttcttct aaagtagacg gaacgatttc attttcaatc actaactcat
     9301 gagagatttt attgaaccat ctcgcttctt gatcagcata ttctgaaaaa agggtactta
     9361 attcttgcga agatgtaccc aaaacataca attgtgcttg gatcaatttc atttcttcta
     9421 atttaaaatt tgctaataca tgccccacca tggcgccagc agttggttta tgatggtcga
     9481 tatctgcttg ttttgtttct tgaacaaatt tttcttccac tatattcatt gatttacgcc
     9541 tcttttactt ttgctgattg gacttcataa cctaattttt ccacaactgc agctaattgt
     9601 tcagggtgga cttcatctgg atcaaagttt gctttgattt tgctcgcatt aaacaaaact
     9661 ttcgtattgc ttaccccagg ctgttgagaa accgcatgtt cgattttttg catacagctt
     9721 ggacaagcta gtgtatctaa ttgcataata atttttttca tcgtagttct tccttccttt
     9781 ttcttgatag tcttattcta gattacagct tccataaaaa aattgattct tatcaagttt
     9841 tacgattttt cttctttgct ttcgtgtcca aaatacaata aacgcatcgc gttaaagata
     9901 acgatcaaga tactagcttc gtgaacgaac atcccactag ccatgtaaat aaacccggca
     9961 aataagccgt tgagtaaaaa taagacgaca ccaatggcaa tcactacatt ttcacgggta
    10021 tttaaaacag ttttcttagc caagcggtag gcatgaacca acgattcaaa attagattgc
    10081 atcaagacaa cgtcactcgt ttcgatagca acatccgttc ctgatcccat agcaatcccg
    10141 atatcagccg cagccaaaga gggagaatca ttgattccat ctccaataaa agcaacgcgc
    10201 aatccttgtt cttggtattt tttcacaaat tgaacctttt cgtctggtaa taattcagca
    10261 tggacttcat caatgcctaa catatcagct acataatcag cagtcatctg attatcacct
    10321 gtcagcatca ctaactgttt tgctcctgct tgtttcaact tagctaattg ttcagcgact
    10381 tctggtcgaa tcacatctga cacacctatg atttgagtga tttttttgtc gattgctaca
    10441 ataatgaccg agctgcctgt ttgttgtaat tgatgtaaat cttgtacttg ctttggtgtg
    10501 agagaaagtt tgtgagcggc aatcaatttg gtgttgcccg cacaaactgt atgaccatca
    10561 atctcagctg tgattccttg tcccttgatt gtctgattat ctttgactgc taattgttga
    10621 aaatcaatgt ctttctcctg agcataatgg atcacggcgc gccccagagg atgatcggat
    10681 aacgtttcta atcgagccac taaagctaag ctgtcccctt catcctctgt atagttcttg
    10741 atagtggata cagcagtagt tcctttggtc aatgtaccag ttttatcaaa gacaaaagta
    10801 tcgattttgg aaaaagtatc cattgcttca ccgcctttga ctaagatgcc ccgtttcgct
    10861 ccattaccga ttcctgctac attcgaaaca ggcgcaccaa tgactaaagc gccaggacaa
    10921 ccaagaacca aaacagtgat tgctaaacga aaatcacgac taatcagacc aacaaccaac
    10981 gcaataagta aaacggttgg agtataatac tgagaaaatc gatcaatgaa tttttccgca
    11041 tgggatttgg tatcttgtgc ttcttctact aattcgataa ttttggcaaa ggtcgtatca
    11101 tctccgacct tagttgcttc aatcgtcacg tagccatctg ctaaaatcgt tccagagaag
    11161 acttcatctc cactgatttt gacgacttgt cgtgattcac cagtcactgc ggcttcatcc
    11221 gcataccctt tcccgtcaat gactcgtccg tccacaggaa tagctccacc agtttttact
    11281 aagacgatat cgccttcttc cacgtcatcg atttccactt cttcagtcgt tccatcttct
    11341 tggacaacac tagcagtaga tggggccatc tcggtcaaag ccttgattga ctcccgcgtt
    11401 ttggtcaatg ttttttgttc cagatagcta ccaaacaaaa acaagaatgt aacgatagct
    11461 gactccttgt attctccgat cataaaggca ccaagcacag caatagtaac gagtaaatca
    11521 attgagataa ctttgacttt cactgcctga tacgcatgga tcataatagg tatgaccgca
    11581 ataatagaag ctatggctag agcaatgtta tagaacaaga caaaaccagt aaattttccg
    11641 ataaaaccaa aaataatcaa cacagctgat aaggcggtga tatgatttcg atgctcttgg
    11701 atccactttt gtaatttcat ctttctccct cttttccttt cattcttacg agactaagta
    11761 tagatactta tccatcataa aaaattgacc actatcaagt ttagtcaaaa aggcggtttt
    11821 cttttggtag aatacactac aaaaaatagt tagcaagaag actcactaaa agtgatcttg
    11881 ccaactattg tcgtttctat cgaaattttc attgaatgat tttcctgtac agcatacatc
    11941 ggagatctat ttctttatct aagattttta tcttgctaat tttttccttt agtctttcaa
    12001 atccacgtcg ttcatagaat tgatacgtac agccactatc tgtaaaaagg aatatttctt
    12061 ttcctttctc tcttttttca aattctgtta gcaacttggt accgatgcct tttgttttca
    12121 aatcaggatt tgcagctaaa aaaatgatct ctccatctgg cgaatgcttc tttaaatatg
    12181 ctgcaagcat ttgttcattt gctgtctcgt aagctccttt actttcttta gcaaatatat
    12241 tttgtaaaaa atcgaatatc ttaacatata tttgctgtcc aaaagaacga tgtttctttt
    12301 tttctccttt tatctccgcc agcaaaacac ctgccaactc gtcctctgca tacaaagcaa
    12361 gaatttgagt tgctcgattc aactccatat acaaaaaata tcttccgtaa agattcagca
    12421 gaagttcatt ttccatatat ctctcaaagt tcattccttc aacagcaaaa tagatagctt
    12481 ttttgtaatc tttcttttct aagggtttaa aagttagttt catcatttcg cctcctcaac
    12541 ttcaatgatt gtgttatact tacattataa tagacacagt gtctactatc tataatcaat
    12601 catctataag tttgtcgatt aagcgacagc tttgctttga tatgtcggat aaggagatag
    12661 gtaaatgaaa aagacagata gacgcgtcaa aaaaacagaa aaagctttgg ctgaaacact
    12721 atccacatta ttagtaaata aaaagattca agcaatcact atacgcgaac taacagaaac
    12781 ggcagatgta catcgttcga ctttttatac acattataag gatatctatg atctatatga
    12841 tcaattagaa agcaactttt ttcgggatct aaatgaaatt ctctcttatg atccagccca
    12901 tagctatgag gaactataca ctcgcttgat tgactatgtt tacgcaaata cttttttata
    12961 tcgcttattt atcctcaacc atggtgatat ttcgtttcag aagaaagtta aacaagttat
    13021 tgaagaaaac tatctgaaca tatggctata tgaagacaag aaaacgatta tcacagaaga
    13081 aatgagattc tttactacgt accacgtcca aggttgtcta tccgttctca atcggtgggt
    13141 agaaagcaat tacaagtact ccaaaaaaga attattagaa ctcttacaga aactgaatga
    13201 tcacatagag aaaatcatgc cttgaataaa aacatactaa gcttagttaa gaagaaccgt
    13261 aaccttctcg tataacaact gaatcttatt tagccaaatt gtttactgaa gattttttga
    13321 gtaaaatttc tataattcct acttattgaa aagtagtttg tagtgctgct tctccctctc
    13381 taagtgatat atcttgttgt accaatcaaa ttcacctcat cattaataaa ttacttttag
    13441 gctaatttat tacaacttat aggttgaagc tgtaaccaat ttatagtcat attatatttt
    13501 tatcatccag tttattgaat tcttctcgtt tacctcaatt cattttcaag gggaatcagc
    13561 tcatcatcta acattttacg ccatgctcgc ttcatttctt gtgggtattt aaatggtttc
    13621 gcttctggat gtttggcttt atatccttcg taatccttac tgttgataat tgctttcata
    13681 ttaggaattg gatcttcacc tggcatatat tgcagattag aagcatgtaa ttttttctca
    13741 gaaacaaacc actcattgga aaagacatga atcaaatcat cacttacttg tgtaaaatag
    13801 cgacgtttaa tgacaaagat atcttcattg ctatctagtg taccttcatt tctttgtgct
    13861 taaattcaaa gataattttc tcaataaaga tttttgattc cagtaagatt cctttactcc
    13921 ttttacaaca tagcgttgtc ctttgttttg aaacatcttc ttcccctctt tgccattaaa
    13981 aatagcccaa ttctctataa agaagattaa gcttcattat tataatataa agtttaaatt
    14041 tggtttatta cttacttttt cttattctct agtaagctaa tattgaaaaa aatgtaatca
    14101 gatcattatt tttatatgtt gtaacttcct gataagaaaa ctaattaaaa gaaagaggta
    14161 actacataaa ctaattaaaa aagctaactt catagttgga ggataaatgg aagaaacttc
    14221 aatgattgtt tctccttgtg ttccttcaat cgacactagt cctattatca atctctcttt
    14281 tttgtccatt tacttttgcg actgttcccc aataatcaaa tacaggaagc gttatttttt
    14341 gtcctttaga ttcatgtact gtatagacta ttttcgaatc catcacttga taaggcatag
    14401 tatgccattc cttgccaatt cttacttcat gattctgcaa atgttctttg tattgtaatg
    14461 cttccgtggg tgcatagtca taaagtcctc cgggaaggag tgtcgtgtaa cgaataagat
    14521 tttcttgatt cattaccaag tgatctctct gagtgacaac tttatgttca tagtttgctt
    14581 ttgttgcagc agatacagcc aacagtaata aaacaattgc cgttatatta actacttttc
    14641 tttgcttagc tgatgtgctc ttcatgaaaa cgaacgataa aatcaacgag ccaaacagta
    14701 cttgaaagcc taagattcgc caaggaaatt gaatcatttg aatgggtgta ttttgtaata
    14761 aagaccatgg aaaaaatttg gtggataaga gccaagagac taccgcacta aggtaaatca
    14821 ctttttcttt gcgattaaat cctttaaaaa agaagagtgg acagatcaaa gaaataatac
    14881 caatgattcc taccgtaaag gttgtcaggt cattattcat cgcaccaatc aaactatccg
    14941 agacatttaa tgcttggtgt tgtaaggtcg ttttatctgg ctcattaatc gattgaacca
    15001 tgatttgtcg gaacatcgga taccaaaagt atgctgttaa aagaatcgtc attcctactg
    15061 cctttgacaa ctccacaatc aaccggatgc tttctttttt tcttgtttca cttactacaa
    15121 agccgcctaa gactagtaag ccaacggcta ttacactcat aaaagtggaa agaacatgtg
    15181 tataaatgag taagctcata ctgactgcta atggaattgc acatggcttt ttctcataaa
    15241 tcagtgcgta agcataatac ataactatcg gtataaccgt aagtgcaatc gcttcagcaa
    15301 tagcagaacg atgataaata tcaatcgtac ggtacccata aaatgtatat aaacagctaa
    15361 atataaaagc cgcatcgatt ctttttaaaa attttagtcc ataatgaaac atcagcatga
    15421 tagtacaaac attcaatagc caaacatata agatataact aacgattaaa ttattactta
    15481 tccaataaaa aatgactgcc ggaaaaaagg tcaagtaggg ataaaaataa ttcactccgc
    15541 tcccataatg attaaacgtc gtaaaattga ttggaccaga aaaaattgaa gataaccctt
    15601 tgactctagc aaaatggaag gcaatatccc ccgatgtaga aatagtatga acatttctaa
    15661 aataattgat tgaataaagt ccagcaataa ttgctaggag aatggacaat attatttttt
    15721 tctttacttg cattacttaa ttatatactc ctttcatgga ttgctttatc tagtataact
    15781 taaattttaa ataaatcgtt atttattttt actctcactt aaaaaagcac taaaattata
    15841 agagagggag gtaatggtct gaacaccaag aattgttcca acatcgaatt gcttaactaa
    15901 tgagttcaaa gcttaattat gtggctctta tatttaccga gcgtggattg cgatctcttt
    15961 agcgaaacaa acatctttca taaaaaaaac agccatatcc gctttcttgg atatgactat
    16021 ttactactac tttactatct ttcattcttc ttgtctcact tatgataagg cgctgggtat
    16081 catgagtgaa tgatattgct gaatacaaat agtccgacca cattaagttg atcggacttc
    16141 ataagcattt tattgactaa tacttttttg cttaagaatt gattctaaca tcttttgcta
    16201 aagttaagca tcctatagtt cctgcattat ctgctaactt aggtgtgaca atgtattctt
    16261 cgatatttgg cgtatcgaca tagccgttaa ttagttctaa aaaacgtctt tgtactttct
    16321 ttttcagatg ttcttgttgc atgacaccac caccaaaaat tataatatct ggtgaaagca
    16381 tcagggtcgt attacaggca cattgtgcaa tataatccgc ctcgatttcc cagaacggat
    16441 cttctataga aagatcttgt cccttggttc ccgtacgcat ctcgatagcg ggtccagatg
    16501 ccaacccttc gagacaatca tgatgaaaag gacaagtacc agagaaagta tctcctgaat
    16561 gtctcttaac taacgtatgc cccatttctg gatggctaaa cccttcaaca aaaatcccat
    16621 cttgtatagc tcctccacca attccagttc caacagtgta ataaacaaca ctggataaac
    16681 cttttccatg acctgaaaca tactccccgt aacaagcagc attcacatcg gttgtccagg
    16741 caattggaat ttcgaaatgc ttattcaacg ttccaacaaa atcaaaattt ttccaggcag
    16801 tttttggagt agaggtgata tgtccatagg tctttgattc acgatggata tcaatcggtc
    16861 caaatgaccc tactccaatg ctttctaatt gttctttata gtgattaaag aaatcaatta
    16921 ccaaactcat tgtttcattc ggtgtagttg taggaaaact cactctttct aaaaccttta
    16981 aatcatcatt gccaatggca caaacaaatt tagtcccacc tgcttctatt gagccataca
    17041 tttcttactc caccgtcctt ttatctagga attcgttctt ccctgtctaa tagtttttcg
    17101 aaaaactcat gtggttcctt ttggtaccaa taggcaacag aagaaatatc gtcttgacgc
    17161 tcataaagct tgatgtcatc atttcctagg gtttggaaag taacacgtaa attttctttg
    17221 aaactaatgg ggtcgggtaa atgccagcgg tacaacccat gcatcggtaa agcatcatcg
    17281 ccaaaaccat gaactggatt tggattaggt tttccagcac taaaataatc ccgggtgtga
    17341 tctcttttag attgaaaagg atagcccata aatagcgtat taaaattttg agcatatgcc
    17401 ctttctccaa agttcttttt atggaaagcc caagcgccgc caaaataatc ttctgctcct
    17461 gtagatgtta ttgtagggaa atcttcatct ccatctatat aaaacttaaa ttctccttcc
    17521 ccccaccagt atctttccaa tgcgcacaaa gcaaagtagg ttcctacata atacccagtg
    17581 cctttgattc catcaataag ggtgtaatct tttccttttg tcgtgatggg ttcacggttc
    17641 catttagcat gaaaatacat cgcatcatcc ggtacttcag tcaatgcata attaaatgta
    17701 taaaaaatgt gcttaataaa ctcaggatgt tcattcgtta aagtaatctt agcttttttt
    17761 cgaaatggca tctcaaaata gcaattcatc cctgatgttg gattcactac gattggtaag
    17821 gaattcactt cgcatcttgt cccaaaccca ttacaaaaaa aatctcctaa tggactttct
    17881 acagaaggag tggattcatc atcccaataa atacgcaaaa ttaaatcacg taagacgaaa
    17941 ctcccagcga ttgttttgtc tgtcactgtc atccaaaaat gacgtaaaat tccagggcct
    18001 tccatttctg ctaaagtaat tgtctctcca gcatttatcg gaacagaagg tgaccctttt
    18061 ctcccaattc ctagatgact cgctgtcatt gccgctttcc ctttttcacc atttttattt
    18121 tctggtgaaa tagttctact ttcctctccc ttgtggagcc atatattatt taaacaattc
    18181 attttattcg tcctctatcc tttcttcact tttttgatta ttcatataga tgatttgttg
    18241 ttgtgcctcc gcaataattg ctccaatatc taaattatca tttcctaact gcaaagcgat
    18301 tgacattgcc aagggaagat tcattcctgc aattacgtgc actctgtcag aaatataggt
    18361 aaaaaacttt tgatttacac ttcctccagc catgtcagtt aaaacaatta tttcgtctgt
    18421 agtatccact aactctttaa tttgttcatc tatatcatca atttcttcga catatgcaca
    18481 taagctacta atttgattat gcatacctgt taaaaactct agtgtctctt taaaaccctc
    18541 agctagtatt gaatggctag caatgatcaa tcttctcata attcaaatcc ttttccttta
    18601 cgctaatatt ccaaatgcag ccataaccat cgcaaaaaca ataacgagta gaatcaaaat
    18661 ggtcatactg atttttttct tactgagtag ataatatgct gcagcagtaa taattactgg
    18721 aattaatgtt ggtaaaattt tgtccaacat tggttggatt ttcatactaa cttcaccaaa
    18781 agtgaattta agtggcgttg aaacactgat aactgaagca attaatgatc caactactgt
    18841 tagccccaat actgaagcag cttcagttaa ataattaatc ttttcaccgt attcagtaac
    18901 aatcttcatt ccctgcttat aaccaaattc aaagcctctc attcggaaaa agaagattac
    18961 catgattaaa atgaaccata aaaagattcc tacaggatta ttttctaaag ccatataagc
    19021 cgcaattgat cccatgatgg ttgggatcat cgtcatcaaa agagagtccc caataccagc
    19081 aattggaccc atcgtaccaa ctttaaagtc attaatcgca tcttttgctt ctaatccttg
    19141 cttttcctcc attgcaacac aagcacctaa aataatggct gccagccaag gctgtgtatt
    19201 gtaatactga aagtgattat ttaaagcttc gatgtattca tcatcatctt tgtataattt
    19261 tcttaaaata ggtgacaatg cgtaagtcac agatgcacct tgctgtgttt gataattaaa
    19321 ggtacaaact gacatcaacc aacgatagtt cgctttccta agatcctttt tcgttacttg
    19381 atatttttcg ctcattttat tcatcctccc caataaaccc aacatcagaa gctcccgcag
    19441 aagcagtgat ttgtgcatct ttttcagctc tatgatacac attaaatgct aaagcgaatc
    19501 ctatgatcgc cgctccaata ataggtacct tcaaaaaagc ggctaagaaa aatccaacaa
    19561 gtaaataact aatgtatcga cctgtaggca taaagcgtaa caacatcgca ataccaacag
    19621 caggcaacat ttttccggct aatgttagcc catccataaa ccaattaggt aaaacatcca
    19681 aaacgtaatt aacagcagac tgtcctaaag taacggcaat aaatactgga attgctgcag
    19741 ataaaccaaa gaaaattggc cccaaccaca aaacactatt cattttctta aattctcgtt
    19801 tattcgcata ttcctgtgat ttgcgaacaa caaaaccatt taagattttg acaattacat
    19861 ctagttgcac ccctaacata cctacaggta gtcctattgc taatccggct tctactccaa
    19921 gctggctacc aaatgccgtt gcaataattg cgcacgttcc atagtccggt acagatgagc
    19981 caccaaaacc ggcaactcct aatgacatca attgaactgt accgccaact atcaaacctg
    20041 tttttatatc acccatcaaa attcctgtga ataatcccca aaaaactgcg taatttacaa
    20101 agatcatcgg aatcccataa taatcaacat gtttaataaa tgctaatata gtcagcataa
    20161 taatttgaaa aatagtaaaa tgcattatcc ttactccttt atcctaattt tgattgaatt
    20221 tcctgtgcct tatccgccgg aacaaattgt gtaacaattt ctacaccaga atccaacaat
    20281 tttttatacg tgctgatatt ttcatctgat gcataggctc tagaagatag ctgtaatgca
    20341 ttatctttaa caacagtacc gccaacaatt agtcggtcaa ttctaatacc gttatctact
    20401 aaatgaagga ttgtttccgg ctctttcacg atgacaaata ctttttcctg atcgtatttc
    20461 ccctttttaa aattcgcaat cgctgtttct tcagtgataa ttgatacagc cattccagca
    20521 ggtctagcta aacgcatact tgatttttgg atgtcgttat ttgcgacaga atcatcgatc
    20581 accatcactc tgtttgcttt agacattggt gcccattgcg tgacaataat cccatgcaat
    20641 aaacggttat caactctcga cataacaata ctcataaata cttcctcctt tatctttcat
    20701 tttcttacaa gcaattacca tgccaaaaca aaaagaactt gataaatcaa gctcttttta
    20761 cataatcata aacgtattta acctcatgaa tcgggatgtc gacactataa taatgttcaa
    20821 cgacactgaa agattgctta aaatgtgtct gaaatttttg aaattcctca ctcgtttcat
    20881 tgaaataaat ttcatcttcc aaagtatttt gttttactaa tcgttcaatt aagcaactaa
    20941 tgtgaacata taatccgaaa cacgtattat tatcaaatga aattcctaga tcattttgta
    21001 agatgtatat agcatctgaa acccgttcta ataatttagt cggatttaat atggtcaatt
    21061 gctccattaa attcgttaga ctaaaattct tcagaatatt attgttcaaa actttcagtt
    21121 cattatcatt aaagtgtcta ttcaattgat tggagaattt tcttatattc attcctaaaa
    21181 tcatatcttc aattgaaaag aattctacat cttctagatg tggatttagt gttccaacaa
    21241 taaaaagaat attgttctct ttacgaagct ccgataaata gttttcattc actaaagagt
    21301 aatagtcaca cgtttctatg acaagatcag tatcccttgg aaagctatct tcaagtatcc
    21361 tttttaattt aaacgaagtt cctaatccgc ttgcacaaga acagacaatc atatccttct
    21421 taatatttga tgattcataa atataatcca cattttgatt ttcttttact tgttcaagaa
    21481 tctgtttaag cggtaaatct aataatatct gatttgcaac atcaatagcc attcttgttg
    21541 aaacattgtt tagtagtccg aatttttttt gagcgtctag ttccaaattt agataaattt
    21601 tctccagtga acccatatca acaagtaaaa taatttcttc atatccggta cactcttgta
    21661 agtatctatt aattttgtct gcaatctcac tacttgaagt cattagtggc atatcaatgg
    21721 cgtcgtatat atatctctca acgatcctat tagcagtact tgctatacag gttgccgtta
    21781 gatctccatg agccaaaatc agtgctaaac gtttttgctg aaattcttta ctacacatta
    21841 acttaaataa aataaccaaa acattttttt gaatttttgt taaataaata tcccctattt
    21901 gaataaaacg attgactaat tgaacttctc gactacacca actattaata tcattgatga
    21961 aggaatataa tttatcgtcc gatgttaatt ggatttgata aatccaagaa ttcaaaaaga
    22021 tagacgttat ttgaagataa taatagtcaa attctatttg aaaatctctc tctatttcct
    22081 taacatactg tctaaatcga atctcttgat aggtgggata tgttaaagct gaattatttt
    22141 ggatatcaac attcttttgt ctaaaatatc tatcatattt ctcaaaaagc tgctgcttat
    22201 cagtgtcttt cttccacgtt tgataaagat actctataag ttcgttgata ttaaaaactt
    22261 cgtctacttt ttccaaatca tccatttgaa tatattttaa ttcattctgt cttttcataa
    22321 ccttcatgcc gttgaaattc acccattgtg gtaaatgata catctgaata taaagttctt
    22381 ccaaatcatc taagaaataa aaggcattgg cacaggtcaa ttgaatgata tttttcaaat
    22441 tgccaacatt ttcactaaag cgatagttta ataaaaaatc taataattgt tgagatatca
    22501 gtattttctt tgctagtctt tcttcttctg atttaaaaaa tatcttgatt aactgttttc
    22561 tttcataaag agaacgactg tctaatgttg gaagtgttag ctttacaggt attcttctca
    22621 acaaagttga taatagttta tcctcaggtg gttctgatgt tgcaaatata atccagcaac
    22681 ttgatgtgta ccagttttca ttatcaccca tccgatgaaa tttcctcgta tccataaact
    22741 gaaaaagttt ctcctgacat tcattgctca atgcatgaat ttcatctaaa aataagactc
    22801 cattattagc taattctgct aaaccctttc ttccatcttc ggctcctgta tatgcacctt
    22861 ttacatgccc aaaaaaattg gacaagaaaa gttctggatt attggcatat tcactacaat
    22921 taactgaaat gaaagatgcc ttctcttcta tataattatt ggccaacaaa tccttataca
    22981 cataatttgc taaatagctt tttccagttc cagtctcgcc atgaagtaaa atgggtaagc
    23041 catttggcgg atagtagatt gctgctttaa tttgagcaat tacatttcgc agacttcctt
    23101 gacttccgat aaccctttct attccaaccc taaaagagct atcataccat ttttccgctt
    23161 cctctgaaga gctaaaaatt tgttgatttg gaactttctc cggtaaagaa aagaagatta
    23221 ctggacgagt attgattttt ccaaattctc catcttttac gaattcattc aaatattgag
    23281 agacaatatt acgactaaca tttaattttt cagatatttt ctgtgctgta ttgttccccc
    23341 atttattttt aatctcagag ttctctatga cataattgac aatgcgttct tttaattctt
    23401 ccatcacagc acctcctcga cactattcga attttgccga cagtattttt ccataaatat
    23461 gacttgctaa aaaggctttg tttagatcat tcaattggaa cgttgcttgt tttgcttgat
    23521 ccgctaagac gttgaaaaga tactctggcg aaatcgcata ccccaaaata tcaacgattg
    23581 ttgcaatcag atcttctttt gattcttcat ctgaaagtaa ttcacgatac aacatcgttt
    23641 gtttgcttac tgtgtcatat tcctctaagg attcatctgc taaaagaacc acttgttcca
    23701 acaaacggtc ggataaatat ttatagaaaa tcagccccaa taaataattt ttatattctg
    23761 acgcatccat tttactacgg agattatctg ccgcactaaa taacttctga tttaattctg
    23821 cacccaaaaa gatacactcc tatctcttat ttaaagttgc tatataccct cttattttag
    23881 catactcagt tttagaaaaa gcaaaaaaat aaagaaactt attcttgata acaagctacg
    23941 tgacgtatat ccctaacaca aaaatagcca tatcctttgg tttggatacg gctatttgat
    24001 attactcatt caattatcaa atcacagtag gtgtgaatct catcgggatc ggcaatatct
    24061 acttctgatt aggatcaaag gtgattttct caataaaggt gtgatacaaa gctttaactt
    24121 gcttcttctc actctggata agaaagcaat ccaaactagt caatatctgt tggacatctt
    24181 tgacttgaat cggttcccct tgttgttgaa agatccctaa gatttcattc tctcgaattt
    24241 gtaactgtct tcgtttttca gtcaattcat ttaaacgatc ttttaacatc ggaaacaatt
    24301 cgggactatc ttccaaagca gcttcctgtt tttgctctaa tagttagatt ttgcgtacca
    24361 ttttcttctt ttattttcct tcgattgctg attttaatat agtctaaagg aaatttatat
    24421 ttattgtatt ctttataact tcaagagaac ttcgtttgag tattactgta tttttttaat
    24481 tccataagaa aattagttat tacctgccac ctaatcagca aatctgaacc tgagtcaata
    24541 taattaatat tattttccat ctccaacatt gtaagtactg ttggtgttaa tatagggact
    24601 ttgtacaatt gtattttttt gattttatca tctgcacaaa ggagattttt taaatctaat
    24661 ccttctttta tttcaaaagt tgaatttttt aaatctataa gagtaaaacc aattccttca
    24721 tactgaattt ctgaaaatcg aatttttctc ccagttgata gatcaagggg cagaaaacct
    24781 tttattacat tgtgagtgtc accatcattt acaatgtcat taggaatttt gattttagaa
    24841 ttctgtggag cacctattgg aagataataa ttcattataa ttttgtagtc ttcatgagga
    24901 aataaagtac ttttaggatt gctaagatat tctcgtaccg aatctaagaa tggcttatcc
    24961 attgaatctt catccccatt taaactaaaa aacattccta caattgcttt tgcaactcta
    25021 agtgaattca ttttctttat tgaatgtgtt aaatcagact taccattctt taaacttgta
    25081 ttgttataaa ggaaattttt attgtcttct gcaaatctaa tatattcctc aacatactca
    25141 ctaccaaaaa aactattaca tttactacat aacgtattgg aatggcaaca gtttttttga
    25201 caaattttat aaggtgcaga acttctttcc gtatgctatt ccgattgtcc ttgacaatga
    25261 gcctcctcgg atgtgacgat cttcggcagg agctaacagt taaagttaga ctgcctcgct
    25321 agcgttagca catccgagct cattatcaag gattcgctgc gccaat
//
LOCUS       DAZBTF010000035        24561 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000035 DAZBTF010000000
VERSION     DAZBTF010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.035"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..1018
                     /gene="rapZ"
                     /locus_tag="ACHGNS_001547"
     CDS             134..1018
                     /gene="rapZ"
                     /locus_tag="ACHGNS_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFN6937494.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            1015..2013
                     /gene="yvcK"
                     /locus_tag="ACHGNS_001548"
     CDS             1015..2013
                     /gene="yvcK"
                     /locus_tag="ACHGNS_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFN6937495.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            2047..2982
                     /gene="whiA"
                     /locus_tag="ACHGNS_001549"
     CDS             2047..2982
                     /gene="whiA"
                     /locus_tag="ACHGNS_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFN6937496.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            3550..4206
                     /locus_tag="ACHGNS_001550"
     CDS             3550..4206
                     /locus_tag="ACHGNS_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937497.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            4385..4950
                     /locus_tag="ACHGNS_001551"
                     /pseudo
     CDS             4385..4950
                     /locus_tag="ACHGNS_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5649..6170)
                     /locus_tag="ACHGNS_001552"
     CDS             complement(5649..6170)
                     /locus_tag="ACHGNS_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFN6937498.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            6368..10048
                     /gene="nifJ"
                     /locus_tag="ACHGNS_001553"
     CDS             6368..10048
                     /gene="nifJ"
                     /locus_tag="ACHGNS_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFN6937499.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            10237..11124
                     /gene="cdaA"
                     /locus_tag="ACHGNS_001554"
     CDS             10237..11124
                     /gene="cdaA"
                     /locus_tag="ACHGNS_001554"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFN6937500.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            11121..12290
                     /locus_tag="ACHGNS_001555"
     CDS             11121..12290
                     /locus_tag="ACHGNS_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFN6937501.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            12314..13669
                     /gene="glmM"
                     /locus_tag="ACHGNS_001556"
     CDS             12314..13669
                     /gene="glmM"
                     /locus_tag="ACHGNS_001556"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFN6937502.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICARYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(13741..15546)
                     /gene="glmS"
                     /locus_tag="ACHGNS_001557"
     CDS             complement(13741..15546)
                     /gene="glmS"
                     /locus_tag="ACHGNS_001557"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFN6937503.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            15956..16246
                     /locus_tag="ACHGNS_001558"
     CDS             15956..16246
                     /locus_tag="ACHGNS_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937504.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            16259..17284
                     /gene="sppA"
                     /locus_tag="ACHGNS_001559"
     CDS             16259..17284
                     /gene="sppA"
                     /locus_tag="ACHGNS_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFN6937505.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            17287..17946
                     /locus_tag="ACHGNS_001560"
     CDS             17287..17946
                     /locus_tag="ACHGNS_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFN6937506.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            18103..19035
                     /locus_tag="ACHGNS_001561"
     CDS             18103..19035
                     /locus_tag="ACHGNS_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937507.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            19189..20478
                     /gene="tig"
                     /locus_tag="ACHGNS_001562"
     CDS             19189..20478
                     /gene="tig"
                     /locus_tag="ACHGNS_001562"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFN6937508.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            20725..21975
                     /gene="clpX"
                     /locus_tag="ACHGNS_001563"
     CDS             20725..21975
                     /gene="clpX"
                     /locus_tag="ACHGNS_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFN6937509.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            22058..22645
                     /gene="yihA"
                     /locus_tag="ACHGNS_001564"
     CDS             22058..22645
                     /gene="yihA"
                     /locus_tag="ACHGNS_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFN6937510.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(22719..22874)
                     /locus_tag="ACHGNS_001565"
     CDS             complement(22719..22874)
                     /locus_tag="ACHGNS_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFN6937511.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            23010..24338
                     /locus_tag="ACHGNS_001566"
     CDS             23010..24338
                     /locus_tag="ACHGNS_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937512.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            24349..24558
                     /locus_tag="ACHGNS_001567"
     CDS             24349..24558
                     /locus_tag="ACHGNS_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937513.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
BASE COUNT         8080 a         4218 c         5319 g         6944 t
ORIGIN      
        1 aaaattggat ttggggcaaa tatcctaaaa gatatttggt ccaaatcttt ttgtaaaaga
       61 aaaagaaaac tggtaatgat acgcccggaa tgatataatt ttactatgca caatgtgaga
      121 ggagttcgta attatgacag ataatttgca attagtcatc ataacaggta tgagcggtgc
      181 agggaaaaca gtagctatcc aaagttttga agatatgggc tatttttgta tcgataatat
      241 gccgcctcga ttgattccaa aattctggga attaatcaaa gaatcaggca aagtcacaaa
      301 aatggcttta ggagtggatt tacgttctcg ttccttcttt gaagaaatcc aaaacatgct
      361 gatcgaaatc gaaaatacga acttcatcga cacttctatc ttatttttag atgcttcaga
      421 cgaagaacta gtggctcgct acaaagaaac aagacgagca catccaatgg cgatggacgg
      481 tttgatcact gaagggatac gtaaagaacg ggcgatacta gacgacttga aagcccaagc
      541 ttccgtgatc attgatacga cgacattgac accacgacaa ttacgtgaaa aaatcaatga
      601 gtcattcaaa tcaaagggag accaaggatt tcggatcgaa tttgtatcat ttggttttaa
      661 atacgggttg ccgatcgatt cggatattgt gatggatgtg cgttttttgc ccaaccctca
      721 ttatatcccg gaactaagac cattgaccgg aaaagatcag ccagtctatg attatgtgat
      781 gtcctttgat gaaacgcaat cattctacca taaatttttg gatcttctgg aaactgtatt
      841 gcctggatac attaaagaag gaaaaagcag cttgacagtt gcaatcggct gtacaggcgg
      901 tcaacaccgt tctgtggcat tgactgaacg tttggcaaat gctttaggga aaaactataa
      961 agtcaatgtg acacatcggg acaaggataa aaggaaagag acggtgaacc ggtcgtgaaa
     1021 atgaaaacct accggataag aaaaccaaag atcgtagtgg tcggcggtgg cactggtctg
     1081 ccagttatcc tgaaaagttt gcgaaatcaa ggagcagata tcacagcagt agtgactgtt
     1141 gcagatgatg ggggaagcag cggtgcaatc agagaatcga tcgctatggc tcctcctggt
     1201 gacttgagaa atgtcttagt ggcgttatcg gatatgccac aattctatga agatattttc
     1261 caataccgtt tcaaaaagga agatcagttt ttagccaatc atacgatcgg taatctgatc
     1321 attgccgcag tttccgagat gcggggaagc acttatgagg cgattcaatt attagcaaaa
     1381 atgatgcatg tagatggaca tgtttatcct tcttcagaaa ctcctttgac cttgcatgct
     1441 gtctttaaag acggaacgac tgcagtagga gaatctaaaa tcgcgatcga ccgtaaaacc
     1501 attgaccgtg tgtatgtctg caacacaaat gataaaagtg aagccaaagc ggctcgaaaa
     1561 gtcgtttcag caattatgga cgccgacatg gtcgttttgg gacccggaag tttattcacc
     1621 agtatccttc caaatttagt gataccagaa atcggcgaag caatgctcaa gacaactgct
     1681 gaaaccgtct atatctgtaa tatcatgacc caaaaagggg aaacggaaca tttcacagat
     1741 gccgatcata tacgtgtttt acacaagcat ttgaatcagc cttttatcga tacagtattg
     1801 gtcaatacgg aaaaagtgcc tgaagattat atggacccag agatctatga tgagtatcta
     1861 gtacaagtcg cacacgactt caacggattg agaaatgaag gatgtcgagt gatttccacc
     1921 gactttttgg aattgaaaga tggtggtgtc ttccacgatg gagacaaagt agtagaagaa
     1981 ttgttccgtt tagttttcgg ttcaaaatat taagcaattt attgttggag aaaggaggag
     2041 aataggatgt catttgctgc agatgtgaag aaagaactaa ctggtttgga agttcatcga
     2101 gaacatgcga aagcggaatt agctgctttg ctgcgaatga acggctcttt aggtattgtg
     2161 aaccagcagt ttattttgaa cgtccaaaca gaaaatgctg caattgccag acgtatctat
     2221 tctcttttga aagatcatta tcaggtacga agtgagctac tcgttcgtaa aaaaatgaaa
     2281 ttaaaaaaga acaatgtcta tatcgtccgg ttgaagcaag gcacaaaaga tgtgttgatg
     2341 gatcttgata tcatggatgg tatgatgttc aatggacacg tttcggacga gattatggga
     2401 aattcgcaaa aaatgcgttc ttacttacgt ggtgctttta tggcctcagg ttcagtcaat
     2461 aatccggaaa cgagccgtta tcatttggaa atattttcta tttatgaaca gcataaccaa
     2521 gacatttgcg atatgttaaa ttattacggt ctacatgctc gaacgcttga acggcgcaat
     2581 ggctatattt cttatttaaa aggagcagaa aaaattgctg atttcttaac attgattggt
     2641 gcaaccaact caatgctccg atttgaagat gttcgaatcg tacgggatat gagaaattcg
     2701 gtcaatcgtc tagtcaattg tgagacagca aatctgaaca agacgattga tgcggcttct
     2761 aaacaaatcg aaaacatcga attgatcgaa gcaaaggtcg gtctacatgc ccttcctgaa
     2821 aaacttcagg aaatcgcaga acttcggctt caacatccag aggtcagctt gaaagaatta
     2881 ggggaaatga ttccctcagg tgcgatatca aaatcgggaa ttaatcaccg aattcgcaaa
     2941 ataaatgaat ttgcggaaaa actgcgtgaa gaagcagttt aatccaaaga aaaaactgat
     3001 acatgaagca ggagatgcga atgtatcagt tttttttaaa agttatactt gcttttcttc
     3061 cgtcaaacta taattccatc tttactggag taacataatt tggaaaggct tctattgttt
     3121 ttaatacaat cgtataaaaa gcggggagac aactgaatga agattttagt tttgtacata
     3181 aatttttgct taatatagaa gtcctgatat agataagatc aagtatttat ctataactgg
     3241 cctgattaat taaaaaactg gaaggaactg ctcgatcatc cttgagaact gctcttctta
     3301 cttctggtca agattctcgt ggttggtgga taaggatggt tgataagttc attggattag
     3361 ctagtcgaaa acaaccaggt gtaattaatg tagaagtttt aacaagggga tggagtgatt
     3421 tattcagtga taatcataag ttcataaata gtaattgaga tgtatggttg gaatacgtta
     3481 tacctgaact tgaactacca agatttggtt tagaatttga gaatcgatgt ttataaaggg
     3541 agggcaaaaa tgcgtttttt tacaactttt actatcatta tgattgcagt actttttatt
     3601 tttctagata tagcaaagcg aaatacagca tttttattat atcgggtttt acttcgagca
     3661 ggtctcataa catttatatc gatcgttgga ttttttctat tcacagttat tgtatttatt
     3721 tggagaactc cagcaccacc attgccagag attacatatg gagaatttcc ttttagactt
     3781 gagtatgaat tgaatgaaga attacatgtg atagaagaca cgttaatagt tgaatttgat
     3841 ggatttggaa tgaacgaagg catagggaga tatcgtagat ggacaagtcg tttagccagc
     3901 ggagaagatt tagtgttgct gttagaagtt agtgataata agcaaatatt ttattttcca
     3961 ggacctgcta attattacat gggagatagg ttgaatggtt ataatcatac ttttcctagt
     4021 gcttctttca ttgaaaggga aagaggcatt actagaaggg atattttaca tgacaaagaa
     4081 ttattagaac aatttggtcc gcttgatcaa aatacaataa atgaagaaga attgttaaat
     4141 caatataata taaggctagt gaattgggaa atatctgaac cgattgttaa caattttggt
     4201 gattagaaaa caattacgat aaatagaaac aagccaaatc atcagcaagt aaaagctgat
     4261 gatttggcct ttcttatacg agtagtagga attcatagcg aatattatta cgatcagtag
     4321 atattttgat attaaaggag tattacgaac tagtatgtga gaatgtagag gtgatttgcg
     4381 aataatgaga aatttatcaa atagaaacaa aatattaatc attattgttg ttatagctgt
     4441 gtttcatctg ggaacgaatg cggttttaag tagaataata ttgggtccta agccaccaag
     4501 acctgcgatt acccgagggg agtttgattt tagattagaa tatgaagtag atggggaaag
     4561 gattgtgata gaagatacaa ttgttgcatt atttgatgga ttttctgcag atgcaggtag
     4621 catggcatgg tatcgcacgt ggcgtttgca tttggcgagt gacaagaggg cacaacaatt
     4681 attgttacat gagttagatg atggtcgaaa aatatattat cattttggag gagctggatt
     4741 ttatatgggg aagttagtag cagtgtaact gtaacaagga aagatttgcc aggtcttggc
     4801 attcgaaact ccatatttta tatcgagcgt gggagagata catctattgg gaacaggaaa
     4861 gggttaactc ttgaagagtt atataatgac tttggaatcc gtcttgttag ttgggaacac
     4921 aatccaccaa tagaaaatcg ctttgaataa tcgaaatcta ttaatccatt tcatcagcca
     4981 gtgaatgttg taatggaaca atttggttta gtgttcaaaa ccattgaaat actaggctgt
     5041 gatcagcaaa aatgtatctg aataaaaaag agagcaacta ttaaatgaga ggagttagga
     5101 gttgttagct ttatacgaat caaagtggca aatacagatg cgtatgcatt tgtgtttgcc
     5161 atcttgcgtt tggtagacga gataggtata agctaaagaa acaacttgtt taaaataaac
     5221 gacctagctc aacaaacgac catctaatca actaacagca tacataaaag caactatttt
     5281 tgaagtagaa ttgaaatcgg gataacttaa aaagtgattg ggatactagt gtgaatcacc
     5341 gtacataaat attactcgtt aagggaattt gagaaatttt gtatgaaaat ttaagaatta
     5401 gttagtatgc gcaagtcaaa aatatgaata aattatttgg tggagataat ttgctcagct
     5461 tgtcataaga aatgagataa tctggcagat gcaagctttt ggacaccatt tattcggaat
     5521 aaggaggctg ggacagaagc gtttaactcc aagaaataag aaggagttgc ttctgcttcc
     5581 gccgtttata ggtgtttaga gcgtgagaca aaagtgttct ttacttttgt cctacgctcc
     5641 ttattttttt attttgtttg gatcaattct tttaattcct ctattgaaat agattcatca
     5701 aagatttctt tacccaagat gacagtcgga acaaatcgaa tatgtgcttg ctcagcttcg
     5761 ttgatgacag cttggctcag ctgttcgtct ttttgttctg tcagctttag tttatctact
     5821 gcaaaatccg ctacttcttg gagagaaaga tgtttccact cgtcttgagt atcgaatatt
     5881 tgtttgatct ctttgatcgc tttttgacca tcgctgattg tgagataccg gtgcatcacg
     5941 tttcctcgct gcaagctttc tttttcttta tcgaacaatt tgatcactcg ctgcaattgc
     6001 ccgctttgga ctgcttcttc taatagctca taagattctt caaaccattg tcggcaatac
     6061 ggacaagata ggttgatgaa ttcgaccatt tttttaggag cattcgatga accataaagg
     6121 attccttttt gtgtatttgt tttcgttgca tcaatcactg atatatccat gttaaaacac
     6181 ctctttcgct tatagtttat acatgtctga caaaaaaagg caacgattta gactgtttgt
     6241 ctaaaaatta gatgaccaat tcaaatagac atatgtgaaa tatttttata aataaaagaa
     6301 aaaatggaat gttttacgtt ataatggaac agataataag agagaatatg aacaaggatg
     6361 atgaatcatg cgaaaaatga aaacaatgga tggcaatacg gcagccgcat atatttctta
     6421 tgcatttacc gagattgctg caatatatcc aataacaccc agttcgacca tggctgaatt
     6481 agtagatcaa tggtcagcag caggaaaaga aaatgtattc ggtcaaccag taaaagtagt
     6541 agaaatgcaa tccgaagcag gagcagctgg tgtggtccat ggatcattga aaactggtgc
     6601 actgacaact acatataccg cttctcaagg gctgcttttg atgataccga atatgtacaa
     6661 gattgctggg gaactactgc caagtgtttt ccatgttgct agtcgggcag tgacgacaaa
     6721 tgcgttgaat attttcggag atcacggaga cgtgatggct gcaagacaaa caggatttgc
     6781 tatgttagct gaaagcagtg tacaagaagt gatggattta gcacctgttg cccatcttgc
     6841 ttctattgaa ggagaagtac cttttttgaa tttctttgat ggattccgta cgagtcacga
     6901 gattcaaaaa atcgaagttt tggaatatga agaattagct cctctgatca atcaggagaa
     6961 attagcttca tttcgtaaac gagggatgaa tcccaatcat ccgactgtta gtggtacgaa
     7021 ccagaatcca gacatccatt ttcaacaacg ggaaacgatc aacccctatt atgataaatt
     7081 acctgaaatc gttcaaaaat atatgaaaga gatcaaccga ttacgaggaa cttcttatga
     7141 cttggtgact tattacggag cagacgatgc ggaagaagtc attgtttcta tgggatcagt
     7201 tgcacagacg attgaacaaa cgattgatta cttgaatgca aacggacgga aaacaggttt
     7261 tttgaatatc catctttatc gtccttttcc agtggaaaca ttcgtgcaaa agttgcctca
     7321 tacagtaaaa acaattgctg ttcttgatcg aagtaaagaa ccaggggcag gtggagaacc
     7381 tctcttatta gacgttcaaa gtgcgttata tgattctgaa cttcgcccgg ctgttattgg
     7441 tggtcgctac ggattggggt ctaaagatgt gactcccgac caaatctcag cagtttttga
     7501 tgaattgaaa aaagacccaa gcatacgcaa aaaacggttt accataggga tcgtggatga
     7561 tgtgacttat caatctttgg aaccaaagga aagtctggat ttgacagaac cgcaaacatt
     7621 ccaggccaag ttttggggat ttggatctga cggaacagtt ggagcaaata aatcagctat
     7681 caagattatt ggagatcaca ccgataaata tgcgcaaggc tatttttatt atgattcgaa
     7741 aaaatctggc ggtctgactg tctctcatct tcgttttgga gatacgccga tacgttcagc
     7801 gtatcttgtc gagcatgcgg accttgttgc ctgtcacaca ccagcttacc tgcatagcta
     7861 tgacttggtc aaaggattga agccaggtgg cattttttta ttgaatactc tttggagcga
     7921 cgaacaatta gaaacgcatc tgcccttaaa attaaaacgt tacttggctg aaaataacat
     7981 tagattctat acgatcaatg ccatgagatt ggcccaagaa gtcggtttgg gacgccggat
     8041 caatacggca atggaaacgg ctttttttaa attagctgac atcattcctt ttgatgaggt
     8101 gcttccgctg ctaaaagaag aagccctgaa aagctatgga cataaatcaa tggctatcgt
     8161 agaaaaaaac atacaagcaa tcgatcggac gaccgaactg cttcatcaag tcgcgatccc
     8221 agaccattgg agaacgttgg agatgcctga aaaaatcaat aaaaggaaca ctggttatat
     8281 acaagaaata cttgaaccag tgaatgcaca ggaaggaaat caactctctg ttggtacact
     8341 tgtagcaaat ggaatgacaa ctggcgaaat gcctttagga acggcttcta tggaaaagcg
     8401 gggcgtcgct ttagaagtac ctgagtggat tagtgatcgc tgtacgatgt gtaatgaatg
     8461 tgcctttgtt tgtcctcatg ctgccatccg tccattttta gcagatgaag aggaaatgga
     8521 agaagcgcca gaaggattta tcgtgagaga aatgcgaggc gcagacggcg taaaataccg
     8581 tatccaagtt tcagtggaag actgtacagg ttgtggtctg tgtgtagagg cttgtcccgc
     8641 aaaagaaaaa gctttagtga tgaagcctta tgaagaacag aaagaacaag ctgtgaattg
     8701 ggcttttgca atgacattaa gacaaaaagc aaatccagtc aaaggaaaga atactgtctt
     8761 gtctacacaa ttcaatcaac cgcttcttga attctcgggc gcttgctcag gctgtggcga
     8821 aacaccttat gtaaaattat tgacacaaat gttcggtgat cgaatgatga tcgcgaatgc
     8881 gacaggctgt tcttctattt ggggcggtgc ttctcctgct tctccttata cgaccaatga
     8941 atgtggtcaa gggcctgcat ggtcaaattc tttattggaa gataatgctg aatttggtta
     9001 tggtatgctt ctagcggcac agactcgtcg cgaggctttg gcaagttcaa tcaaagaagt
     9061 attgccaaaa gtatcagaag aactacgaga attgatggaa gactggttgg tacacatgtt
     9121 tgagagcgaa ggaacgcagc aaagagcagc aaaactgcaa gctgcgatcg aaagcgaacg
     9181 atcaattgtc cctgaactgg aggatatata caagagaaag gatctattcg taaaaaacag
     9241 ccaatggatg attggtggag acggatgggc ttacgacatt ggttttggcg ggatcgatca
     9301 tgtacttgcc agcggagcag acgtgaatat tcttgttttg gataacgaag tctattccaa
     9361 tactggcggt caaacctcaa aagcaacacc agcttcggca atcgctaaat tttcggctag
     9421 tggaaagtat gcttcaaaga aagatctagg aatgatggcg atgacatatg gaaatgttta
     9481 tgtggcgcaa attgcttcag gtgccaatca aatgcagacg ataaaggctt ttgaagaagc
     9541 agaaaagtat cctggacctt ctttaatcat tgcctatacg ccttgtatca cacatggttt
     9601 gcttggtgga atgaaagaat cgcttaaaga agcaaaagaa gctgtttctt ctggctattg
     9661 gtcattatat cgatataacc ctgcaatggt cgaaaaaaac aagaacccaa tgactttgga
     9721 ctacaaaaaa cctgatttct ctgctatgcc tgatttcatg aggaaacaag ttcgcttttc
     9781 ctcgctagag aacgcaaatc caaatcttgc aaaagcttta tttgaaaaga ctatccaaga
     9841 cgctaaaaca cgtttttaca actatgctgc actgacggga gaattagaaa aaatcaagaa
     9901 acgacttgaa ccagaggaat ctatcgatgt acccgttaag actagaaaag aaaaaacgac
     9961 ggaccttgaa gcagaagaaa gaagagctgc cagagcagaa cgacgtaaaa aaatggaaca
    10021 agctacaaaa gaaaaaaagg aaaaataaca gaaaaaatat aatgaataat tctctgaagt
    10081 ttaaaaatat ttattgcaga gaaaaataaa acgcatactt gcaattttcc tgaaaattat
    10141 gcgtttttct caagaaaaat acgagcagat cagcctctaa aattaaaaca tatgatatac
    10201 tagagaggaa atctcaaaag agaagagagg ctttttatgt catttcaaat cggggaactt
    10261 tttaatttgg actattggca gcaaatgttt tccggtgact tgttctctct gaattttttg
    10321 gtgaacatac ttgatattct ggttgtctgg tatcttgtat acaaactgat ccaactgctg
    10381 cgaggcacaa aagcgattca gcttctgaaa ggtgtagcta tttttattgt tatccgaatc
    10441 gtagcggaaa tcattggtct gcacaccttg tcttggctga tgaaccaagt cattacgtac
    10501 ggggtgatag ctgctatcgt gatttttcag ccggaaatcc ggagaggact ggaacatctt
    10561 ggacgcagtt cgcttttcaa acaatcaaaa agcgaacagc aggaagatga gaagatgatc
    10621 ttgtcttttg acaaagccat ccaatatatg tccaagcgaa aaatcggtgc gctaattact
    10681 atcgaacggc atactggatt agatgagtat attgagacag gaattgcatt agatgcagat
    10741 atcactggag agctgctgat caatatcttt attcctaata caccgctgca tgacggtgca
    10801 gttattgtaa aagaaggaaa gatcgcagta gcaagcgcct atcttcctct atcagaaagt
    10861 atgttgattc caaaagaatt cgggactcga caccgggcag ctgtcggtat aagcgaagtc
    10921 agcgacgcga ttaccatcgt tgtctctgaa gaaactgggg atgtcagcat tacattagat
    10981 aatgaactga tggctggctt atcccagcaa gaatatttgg caattttgcg tagagaactg
    11041 attgctgaag agaaaacaaa aaacaagaaa aacgtgattc aatctttcct agacggtatg
    11101 actaagggag gggatcgcaa atgatctcaa aagagaaacg gacaagcatt ctttacagtt
    11161 ttattgcatt gctgttcagt attgcgctgt acttcaatgc aaatggccag tccgttccaa
    11221 atacgctttc tggaaatgaa gcgtataatc aaacagttac tggggtgcca atcaagattt
    11281 cttatgattc aaagcgctac tatatccatg gatatgaaaa tgcagtgact gtcaaacttt
    11341 ccagtgcaaa ccgagttcag ttgaatgcag aggcaaacga agatacacgg atgttccgcg
    11401 tcacagcaga tatcactaag ctgggcgaag gaacacatga ggttccgcta aaaattcaaa
    11461 atttgagcag tgctgtcaca gcaaagatcg aaccatcgac tattacagta acggttgaga
    11521 aaaaggtaat aaaagatttc gatgtggaaa cgttgattcc ttcaacgttc acaccatctg
    11581 gatatgaact tgatgatact tccgttagtc ctaaaaaagt ctctatcact acaggtgaca
    11641 aaacattggc agaaatcaaa cgagtagtag ctgatgtgga cgcttcgatg gtcacagatg
    11701 atgggattaa tagtgaggtc ccaatacagg cgctgaatgc agcaggggaa gtattaagta
    11761 tcgtctctga tccagtgcaa gtaacagtga gagctgattc gatcaagcca agcaagtcgg
    11821 tacgacttta tgggatacag caaggaacac cagctgccgg ggtagaaagc tatgatttca
    11881 gtttttcaaa actggaagca gaagtaagcg gctctagcga tttgcttgca tcgatcggtg
    11941 acagtattgc tgtaccgatc aatgtctcag gtatcaatca tcgaacaact cgtaaaattg
    12001 aaataccagt agaagagggg ttaagtatca agcccgaatc agttagtgtg gagattacac
    12061 ccgtgttgca aacgtcaagc agtagctctg cttcgacgag cagtcggaca caagaaaata
    12121 caagtacgag tagaagtatt tcatctgctg attcaggaac ggaaccatcg acaacaacca
    12181 tttcttccag ttcggaatct gggacaagca gctcgaccac aagtacagaa gaaacatcag
    12241 aaacaagcga atcaacacaa atgagcagaa caaattcctc agaaaattaa gttaaaggag
    12301 taattttaca actatgggta aatattttgg aacagacggc gtacgcggtg aagccaacaa
    12361 ggaacttacg ccagaacttg catttaagct gggacggtgc ggaggttatg tcctaagcca
    12421 gcatgaagaa gaaggaaaac gcccacgcgt gttagtaggg cgggatacaa gaatctcagg
    12481 tcaaatgtta gaagaagcgc tgatttcagg acttttatcg gtgggtatcg aagtgttcca
    12541 gctaggtgtg atctctacac caggtgttgc ttatctgaca cgtttgcaaa aagcaagcgc
    12601 aggggtcatg atttctgctt ctcataatcc agcacaagat aatggaatca aatttttcgg
    12661 tgcagatggt tttaaactag tagatgacca agagctagaa attgaagcat tattagatgc
    12721 tgaagtggat gaattgcctc gtccatcagc tgaaggattg ggaactgtag aagaatttcc
    12781 agaaggctta ttgaaatatt ctcaattcct tgtacaaacg atcaacggtg acctttccgg
    12841 tttgaccatt tgtgtcgatg cagcaaacgg agcaacttca acaagtgtta atcgtttgtt
    12901 tgccgatctt gaaacagatt tttataccat gggcacaaat ccagacgggt taaatatcaa
    12961 tgatggcgta ggctctaccc atccagaacg tttagctgag atggtcgttg aaaaaggagc
    13021 agatgctgga ctagctttcg atggcgatgg cgatcgtatt attgcggtgg atgaacttgg
    13081 aaacattgtc gacggtgata aaatcatgtt tatctgtgct agatacttgg cagaaaaaaa
    13141 tcgattgaaa aaagatacga tcgttacgac tgtcatgagt aacttaggat tccacaaagc
    13201 ggtggaagga atcggtttga aagatgtcgt tacacaagtt ggtgatcgtt acgttgtaga
    13261 agaaatgcgc aaaaacgatt ataactttgg cggcgagcag tctgggcaca tgattttctt
    13321 agattttaac acaactggag atgggatgct ttcaggaatt cagttactga atatcatgaa
    13381 acagacaggt aagaaattat cagagcttgc agcagaggta acaatctatc cgcaaaaatt
    13441 agtgaatatc cgagtaagtg ataaaaacgg tgcgatggat gttccggcaa tcaaaaaagt
    13501 gattgaagag gcagaagctg aaatgggatc agaaggaaga atcctagttc gtccttccgg
    13561 tacagaacca ttacttcgag tgatggcaga agcgcctact gatgagaaag ttaattacta
    13621 tgtcgataaa atcgcggcag tagtcaaaga agaaatcgga ctggattaag agatattttc
    13681 ataaaaaata aaagaacaag gaccagtgtc agatgtctga cactggtcct tgttctttta
    13741 ttattctact gtcactgatt tcgctaaatt acgtggtttg tcaacgtcga atccccgtaa
    13801 tagcgttgcg taatatgcaa gtaattgagt cggtacgact gtggttagag tagttagata
    13861 agggtggaca gttggtagca ccagctggtc actttccttt tctaatccct ctgagacaat
    13921 aactagtgtt ttagcgccac ggctttcgac ttctttcagg tttcctcgag tatgcgcacc
    13981 agtcacttct tcactgatga ttccaatcac tggtgttcct tcttcgatca aagcaatcgt
    14041 tccatgtttc aattcgccag cggcaaaacc ttctgcctgg atataactga tttctttcaa
    14101 tttcaaagca gcttcaagag aaacgttgta gtcagcgcca cgtccaatgt aaaaggcatt
    14161 tcttgtatcg ctgagatata cttctgccaa ctcttctaaa taatcttttt gggcgatgac
    14221 cgattccatc gcattagcaa ctaaactcaa ttcatgagtg atatcgaata caagagaagt
    14281 ttcaaattct tttttatcac cgatagcttt ggctaataaa gttagtacag ctatttgtgc
    14341 agtataagct ttcgttgatg ctactgcgat ttcgggtcca gcatgcaata gcagtgtaaa
    14401 ggatgcttcc cgcgaaagag tagaaccagc aacatttgta atcgtaagtg aagggtagcc
    14461 gagacggttg atttttacca gaacttggcg gctatctgct gtttcgccac tttgactcaa
    14521 gaaaataaag aatggttttt ttgaaagaag aggtgtgtta tagccaaatt cactggctaa
    14581 atgaacctca acagggatct gtgtcaattt ttccagagtc tgtttacctg ctaaccccgc
    14641 atggtaacta gttccgcaag caacgatata gattcgatcg ctatcagaca atgtatcgac
    14701 cagcttttga tcaagagaga cacgattgtc ttctgttacg tatttttgag cgatacgacg
    14761 catgataata ggctgttcgt cgatttcttt cagcatataa aaaggatagg tacctttctc
    14821 gatatctgaa gaatctactt gtgcttcgaa tgaagaacgt tctacgatgt cacctgcaaa
    14881 attttgaata ataacttttt ctgatgtgac ggtaaccatc tcttcatctt caatctcaac
    14941 gaattccttc gtatgagcaa gcatagccat tgcatcgctg gcaatcacat tgaatccatc
    15001 gccgagtccg attaatagtg gacttttatt tttagcgacg taaataacat caggtgctgt
    15061 ccgatcaatc aaagcgaaag cgtaagaacc tttgatgacc cgcaaagctt ttaaaaacgc
    15121 ctcttgcgtt gtagtatcct tcgcgaaagt ttcaattaga tgagcgataa tctcagtatc
    15181 ggtctcacca ataaagtggt catttgctaa ataagcaatt ttaagttctt caaagttttc
    15241 gattacgcca ttgtgaacga gcacgaagcg tccactttgg gaagtatgtg gatgggcgtt
    15301 ttcttcagaa ggttgcccgt gagttgccca gcgtgtatga ccaataccta tatttcctgt
    15361 agtatcaata tttattttgt ttttcaaatt ttgaattctg ccttgtgctt tgaccagata
    15421 atcagttgtg ccatctgaga caaagatacc agctgaatca taaccacggt attctaattt
    15481 ttctagaccg tcaatcaatc ctggtgttac attttttaat ccaatcattc caacaattcc
    15541 gcacataaaa aacatcctac tttctataat gaattcgtat gaatgcttcc agctgcagga
    15601 aaatcctttt gttttatctt tgcagatatt ttttgtggat tttctgtaga gtgcagtccc
    15661 tagggccatc cgccgaatct ttcgataaac cctctcctcg tcatctgttt acacagacct
    15721 ggcgctatcc taaaaggtat aactatcctc ctcttttgtt ttttatctgg aaacatttaa
    15781 aatcataacc ttttgcgaat gttcttgtca acaaaaaaag aataaaaaat aaattggtat
    15841 atatcaaatg ctttgtcaaa tgctcttttt atgtcaaaaa ataatgggtt tttatctatc
    15901 atctttttca ggagttcggt acaatagaaa gtaatcggat agaggaggag taaaaatgga
    15961 ttcaaaaatt gagatcatga cactgggaat gttaaaaaaa caattgtcca aattcgaagc
    16021 atcagctggt gtatcagatg atacgaaaat atttttagat actggctggg atagcatcca
    16081 agaaatcgca cctgatgcct tggaagttgc tgaagctcgt gagtttacgg tagaagatga
    16141 attgacaaaa gagtcgtttt ccgggtatgc gcgggaagaa aaagcagagc ggttcgacac
    16201 ttctgagcag tcagaaactg tgatcgtcat aaaaaacttg tattagaaag gagaaagaat
    16261 gaataagaga cgttgggcag cagttctgat tgctgcagga ttatttgtgg tttcgttagt
    16321 ttcagctagt ttgactaagc ctcaggaaaa cgatcaaatt gagggattga acagttggtt
    16381 atatggggat gaagaactag cttcaaatgt gttggaagag ggagacagta cgcaaaaaat
    16441 cgtaaaatta tcagtagatg gaacgattgc tgcgggaggg tcttcaggtt tatttgcttc
    16501 tgaaggttac aatcataaaa attttatgga acaactgaag gcgattgaag aagatccaac
    16561 agtcaaaggt atctttttag aagtaaattc tcctggtggc ggtgtctacg aaagtgcgga
    16621 aatcgctaag aaattggata cgattcgtaa agaacatgat atcccaatgt atgtcagcat
    16681 gaaaaacatg gcagctagcg gtggctacta tatttctgct caagcagata aaatctttgc
    16741 aacagaagaa acagtgaccg gctcaattgg tgtgatcatg tcaggattga attattcagg
    16801 acttctagaa aaactgggtg tggaagatac aacagttaaa agcggagcgt tgaaagatat
    16861 gggttcagga acgcgaccag agacaaaaga agaagaagcg gtgcttcaag catatatcga
    16921 cagcgcatat agtcgttttg tagcaatcgt cagcgaggga cggaataaat ctgaagaaga
    16981 agtcaaaaaa attgctgatg gaaggattta tgatggtgtc caagcaaaag aagtaggttt
    17041 agtcgatgaa ttgggatttc ctgaagatgc tttaaaagct atgcgaaagg aacaacagct
    17101 tgaagatgcg gaactagtag aatattcttc cacgccatct gggtttgcta gtacatggtt
    17161 aggaagtaaa ctagcagaat tccagggatt gaagccttct gagactagtc agatcttatc
    17221 cgttcttgag aatctaggga cagcggaatc accaaaagca atgtattatt acggaggtga
    17281 gtaagcatgt cagagctttc aaatgaaaat aaggaacaag aaaaagataa gcaacagcag
    17341 cctgctgtag gacctgactc caacttttcc caaaaggtac tgagctcttt tgaaagagag
    17401 cctttgacgc ccgaagaaat caaaaagaaa caacgtaaat ggaaaaagta tacggacaca
    17461 agaaatgaac cgccaaaagc aaatgatttt cctaagtatt tctatgcagg tttttttatc
    17521 cgaatgtttg ctttcttggt cgatcttttg tgtatcggag ctattacgcg tttaacactt
    17581 ggtattgcca aaaatctagg ttggcttaat ttttcagatt cttatctgag tatctacgga
    17641 ttcacagcct tacttattta tctagcgtat tttattttat tgacgaaatt caatcatggt
    17701 caaacgattg ggaaaatgat ctttggtatt cgagtcatca gctttgatga agaagaactt
    17761 agctggtcaa ccgttttagt tagagaaggt gcttgccggt tcattctaaa atttcctttg
    17821 ctgatgttag ggtatcttcc agcaatcttt aatcagaaaa agcaacatct aggcgatttt
    17881 ttcactaata caagtgttgt tacgatcaat ctgatcaaag cattcaacca ggaagtcaac
    17941 agctgatgag caataaaaag cagtaacagt tgaaagcacc ttctaaaagt cagatctaaa
    18001 atctagcttt tagagggtgc ttcagttatt tttaattgtc atgctttttt tgttttgcta
    18061 ctattgaaca tgagctgaac atgagcaaga ggaggacact gcgtgaagcc gattgatttt
    18121 ccagaaaaat atgagaattt aatgcgagta gcacaacaag cactagcaaa ccagcaatat
    18181 cagcaggcaa aagaattatt tcaacgagct tacgagttga aagaaagctt tgaggctaat
    18241 agcctgcttg ttttttgtct ttacgagttg gacgagaaaa aagaagcatt gaagcaagca
    18301 ctcttgcatg aaaaacagta tcttcaaaat gaggagtttg ctgaatttta ttttgacttg
    18361 ttaatcagta cacaagattt tttatatgct agaaaattga tagcaagtac agatttttat
    18421 gaagctttcg agcagcgggt cattgaaaaa atccaatttg cagaagaatt atccggacaa
    18481 atggagaggc agaagatcaa ggcactccac aataaatcgg aggaacttcc ttcgttggaa
    18541 cctgcacgtc agcttgcgtc tattgatgag atcgaacagc ttccttatca tgagtttatc
    18601 caaacagcga gtaaacttat tgtatcgcca gaggtccata ttttagcaag ggcaaaatta
    18661 ctggagacac ttaggcaatt gaacgaatgc catcctgttt tctatttgac gattgaagaa
    18721 aagttagtca aagtcatacc aaaagatctg ccaaagccgc agcagcaggc aagttaccag
    18781 caattatgtg tattttcaga tcgttacggt aatgaagatg cgctgcttag ttccgtgtta
    18841 aatgaagaat tcacgttgca atctgcaatt gtttatcctg tctatgatac atacatcgaa
    18901 gacccgaagc gctggtttca actgactgta gaggcgtgta caggaaaaac gatgtatgaa
    18961 ggtacagaag atgaaaaaca agatttttta aaaaaaagag aaaaaatttt gcagcaaatg
    19021 atcttcttcc attagatttt atcagtgaaa aagataaaaa tgttatgaaa aacaaggatt
    19081 ctatccattc aatatgttta caatttgaaa gggttagtgt actatttaaa ggtatgcatg
    19141 aaacgttttt gatgtataaa atgaatttct aaactcggag ggaattaaat gactgcaaca
    19201 tttgaaaaaa aaggcacgaa tgatggtgtt ttaaaattta cgattgcgca agacgaaatc
    19261 caaaaaggat taacaactgc gtttaataaa gtgaaaaaaa gcttgaacgt acctggattc
    19321 cgtaaaggga aagtttctcg tcaagtattc aaccgtatgt acggtgaaga agctttatat
    19381 gaagatgcat tgaatgctgt attaccagaa gcttatgaag ctgctgtcaa agaagctggc
    19441 atcgatcctg tttctcaacc aaaaatcgat gttgaaagca tggaaaaagg tcaagactgg
    19501 gtaatcactg ctgaagtaac agtgaaacca gaagtaaaat taggacaata taaagatcta
    19561 gttgttgaaa aacaagaccg tgaagtaaca gatgctgacg tggatgcacg tatcaaacgt
    19621 gaacaagaag cacaagctga attagttatc aaagaagatg ctgcagctga aaatggcgat
    19681 acagtagtaa tcgacttcga aggctttgta gatggcgaag cgtttgatgg cggaaaaggc
    19741 gaaaattact cacttgaatt aggttcaggc tcattcattc caggttttga agatcaatta
    19801 gttggtcata aatcaggtga aagtctagat gtaaatgtaa ctttcccaga agattatcaa
    19861 gcagaagatc ttgctggaaa agaagcagtc ttcaaagtga caatccatga agtaaaagca
    19921 aaagaagtgc ctgaattaga tgacgaattc gcaaaagatg ttgatgacga agtatcaaca
    19981 ttagacgaat tgaaagaaaa atatcgcaaa gaattagtag aaacaaaaga aaaagcagcg
    20041 gacgatgcaa aagatgaagc agcgatccgt atggctgttg aaaacgcaga aatcgttgaa
    20101 ttgccacacg tgatggttca tgatgaagta catcgttcaa tggacgaatt cttgaacaac
    20161 atgcaacgtc aaggtatctc tcctgaaatg tactatcaat taactggttc tacagaagaa
    20221 gacttgcata aacaattcga aggtgaagca gaaatgcgca caagaacaaa tcttgtgatc
    20281 gaagcaattg cagctgctga aaatcttcaa gcaacagaag aagaaatcaa tgcagaaatc
    20341 caagaattat cagaaacata caacatgcca atcgaacaaa tcaaacgcgt gttgacagaa
    20401 gatatgctgg aacatgatgt gacaatgaaa aaagcaatcg aattgatcac aggtacagcg
    20461 acagaagcga aagaatagaa atcaagtaat aaatcatttc tgaagaagct acttctagaa
    20521 aattgtttat tcgaaataag ggactgtgac acgattttgt cacagttcct tattttttgt
    20581 taagagtaaa aaatgcaaag gtataggtca gttgatcctt tgttaaacac tttttctttt
    20641 tactaaacta tgttatgata gtaaaagact gtaaagtctt tgatagtttc ttgcatactt
    20701 tttgcaagtt aaggggtgaa gaccatgtat gacaatccaa gtagcaatga gaccgtacgc
    20761 tgttcttttt gcgggaaaac ccaagaagaa gtgaaaaaga tcgtagctgg tccaggggtc
    20821 tacatctgta atgaatgtat cgacctatgt aaagaaatca ttgatgaaga attttatgaa
    20881 gaagctgtac gcgaattcac tgaagtccct aaaccactag aaattcttga agtattgaat
    20941 aactacgtga ttggacaaga tcgcgcaaaa cgctcgttag ctgttgccgt atacaatcac
    21001 tataaacgtg tcaatcagca agcacaagaa aacacagatg atgttgaact gcaaaaaagc
    21061 aatatctgct tgatcggacc tacagggtct ggtaaaacat ttttagcaca aacattagca
    21121 aaaacattga atgtaccttt tgcaatcgca gatgcgacaa gtttgacaga agctggttat
    21181 gtgggggaag atgtagaaaa cattttgctt aaattgcttc aatctgctga ttacaatgtg
    21241 gaacgtgcag aaaaagggat catctatatt gatgagatcg ataaaattgc gcgtaaaagc
    21301 gaaaatgtat ctattactcg tgacgtttcc ggcgaaggtg tccaacaagc cttgctgaaa
    21361 attctagaag gaactgttgc aagcgtgcca cctcagggag gacgtaaaca tccacatcaa
    21421 gaatttattc agattgatac gacgaacata ttgttcatcg taggtggtgc atttgatggc
    21481 atcgaaacga ttgtgaagaa tcgtatgggc gaaaagacga ttggatttgg tacacaaaac
    21541 caaaaacttt ctgaagacga aagtgtcatg cagcatatca ttcctgaaga tttactaaaa
    21601 ttcggattga tccctgagtt tatcggacga ttacctgtta tggcagcttt agagaaattg
    21661 acaacagatg atctggtacg gatcttgact gagccgaaaa atgcgctagt caaacaatat
    21721 caaaaacttc tttctctaga taataccgaa cttgaattcg aacctgaagc gttgagagcc
    21781 attgcgaaaa aagcaatcga acgcaatacc ggtgctcgag gacttcgttc catcattgaa
    21841 gaaatcatga tggatgtgat gtttgatatc ccttcaaatg aaaacatcga aaaagtgatt
    21901 atcacaaaag aagcagctga attgtctggt gagccaacag taatctataa agattcagag
    21961 aaaaaagcgg gttgatgaaa agaggctgcc gtaaaagtca ttttggcttt tgcgacagcc
    22021 gcttgcttta ttcatcacct tgttaaggag agagaccatg aaagtccatc aagctgaaat
    22081 tatcataagt gcagtggcac caaaacaata tccagataca caattgccag aaatagctct
    22141 ggcaggacga tcaaatgtag ggaaatcttc ttttatcaac acattgattg atcgaaagaa
    22201 tttagcccgt acttccggta agccaggaaa gacccagaca ttgaattttt atttgatcga
    22261 gaacgcatta cattttgtcg atgttccggg atatgggtat gcaaaagtat ccaaaactga
    22321 gcgagcaaaa tggggaaaga tgatcgagac atatttaacg actagagacc agctacgtgc
    22381 ggtcgtttct ttagtagatt tgcgtcataa gccttctcaa gaagatatcc agatgtatca
    22441 atttttgaag tattatgata tccctgtaat tgttgttgcc actaaggcag ataagatccc
    22501 tcgtggtaaa tggaacaagc atgagagcga aatcaaaaaa gcgttggatt ttgatcgcaa
    22561 tgacacgttt attctctttt cttccgttac aaaacaggga aaagaagaag cgtgggcagc
    22621 aatcgaacaa gcaattgaat cataaaaaga ataaaaaaga cttaccagtc aaactctttc
    22681 ataaaagttt ggctggtaag tctttttttg agcttaactt acttttgttt cttgtctttt
    22741 ttcttttcat tttgtggctg tggttttaga aatcgatcga tctcttcttt tggtgcaact
    22801 gttttttccg gatcgacagc aaatgattcg aaaagtttat ctaaattatc tgtacgttca
    22861 aatttcaacc ccattgtaaa cacctctttg tttcttttta cttagtctat catgtaatcg
    22921 tccaaattgg tttctaagac ataaagtttt agtttttctt tatcgttggt tataattaaa
    22981 ttataaaaag attttaaaga ggtgagaaaa tgattggcga agataaatta ttgatgacta
    23041 tccagctcac agacttaaat gaagaagaaa aaaaacagga aataagcgat tgggctattt
    23101 tgacacgtct gttgatccag ccaacattta tccgctcttt cacacaacaa gcggatccgt
    23161 atgatcttcg aaagcttcaa gaaaaaataa tgctagcatc ggtaagagac gaaagttggt
    23221 tagttgtagg aaatgacgag aacgaatgta gttttcgctt ggaagataat caattattga
    23281 taaaaaatgt attgtctatc tctgttttca aagaaaagca atttttgatt cgagacttca
    23341 ttcagaaaaa aatgattgct catggggtgt tcgcctatat gcgagcttat agcgagttta
    23401 tctatcacaa tacaaaacag atctctcaac ggctattgtt tgagaaaaag gatgaaatcg
    23461 aacatttacc taaaatgaaa caacaaaacg gggaagtagt agtcgattgc aaccaatttc
    23521 caggatacga tcttttttac caaggtttat gtttcacttc gtgttgggaa atgtattata
    23581 gtcgctatta tcatcaaatc atccctaaac cgatttttct cgacatccaa caagtagaaa
    23641 aagtgaagga attagaaaac gaagtcatat gtatccagct ttatcgagac ccctttaact
    23701 ggcaaaagcc aaacaatcaa atgtttcaat cctattttag ggaccagctt gggtttgatc
    23761 atcttgcttg ggacaatggt gtcggtttat tgaaaccgcc attcgttgaa tacatttaca
    23821 ctgaccatac gatccaatct gtccagtatc agaatgcaca aatgcaacct gttcctaaaa
    23881 agaatgcttc attttttgta acaaaaagtt atgatattca acaaggagat tataaagaaa
    23941 ggcgtgttcg cggaacacta aatgctcagg cgtattttcc ttgggttgac gagaatcgct
    24001 cgcgcatgat gtgctataaa gttatcgatc cgactgttgc tttggataat ggaatcgaag
    24061 cttattgtta ttatattagg gaatatctag aagtagaggt aacagatgaa aaatatcaag
    24121 agtatctttt atccttaaga atctatgttc cttcagaatc cctgtcagaa ttaccgttaa
    24181 aggaaatcaa acatagactc tctgatgtta ccttcaaacg aataaagaaa aagcggcgaa
    24241 gtatccaatt cgatgtcaag aaaggtgagc agcatctgcg tgtgcaattt ttagattacc
    24301 gagaattaga acaattgatt actttacaaa aaatatgagg tgaagaaaat ggataaacaa
    24361 ctctattatc gaataatggt tccggcatca ttattatccg tctgtctctt ttttgttcct
    24421 aattttccta ttcgatcagt catcggtttt ttggtcatgt ttggcgggat gggactttat
    24481 tattatcttc ttcataagga aagaaaacct gcttctgttt atatgaaaaa aacaagaagc
    24541 gaaaataaaa aacgataaaa c
//
LOCUS       DAZBTF010000036        24426 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000036 DAZBTF010000000
VERSION     DAZBTF010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.036"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(53..787)
                     /locus_tag="ACHGNS_001568"
     CDS             complement(53..787)
                     /locus_tag="ACHGNS_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFN6937514.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="ACHGNS_001569"
     CDS             1114..1524
                     /locus_tag="ACHGNS_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFN6937515.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="ACHGNS_001570"
     CDS             1517..2218
                     /locus_tag="ACHGNS_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFN6937516.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="ACHGNS_001571"
     CDS             2218..3804
                     /locus_tag="ACHGNS_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6937517.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="ACHGNS_001572"
     CDS             3823..4044
                     /locus_tag="ACHGNS_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFN6937518.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="ACHGNS_001573"
     CDS             4041..4802
                     /locus_tag="ACHGNS_001573"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFN6937519.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="ACHGNS_001574"
     CDS             5182..5691
                     /locus_tag="ACHGNS_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFN6937520.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="ACHGNS_001575"
     CDS             complement(5766..6305)
                     /locus_tag="ACHGNS_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFN6937521.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="ACHGNS_001576"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="ACHGNS_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFN6937522.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7318..8169
                     /locus_tag="ACHGNS_001577"
     CDS             7318..8169
                     /locus_tag="ACHGNS_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFN6937523.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8197..8832
                     /locus_tag="ACHGNS_001578"
     CDS             8197..8832
                     /locus_tag="ACHGNS_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFN6937524.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="ACHGNS_001579"
     CDS             8852..9625
                     /locus_tag="ACHGNS_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN6937525.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="ACHGNS_001580"
     CDS             10033..10830
                     /locus_tag="ACHGNS_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFN6937526.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="ACHGNS_001581"
     CDS             10808..11221
                     /locus_tag="ACHGNS_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFN6937527.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="ACHGNS_001582"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="ACHGNS_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFN6937528.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="ACHGNS_001583"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="ACHGNS_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFN6937529.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /locus_tag="ACHGNS_001584"
     CDS             15998..16558
                     /locus_tag="ACHGNS_001584"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFN6937530.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="ACHGNS_001585"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="ACHGNS_001585"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFN6937531.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="ACHGNS_001586"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="ACHGNS_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFN6937532.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="ACHGNS_001587"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="ACHGNS_001587"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFN6937533.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGVFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="ACHGNS_001588"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="ACHGNS_001588"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFN6937534.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="ACHGNS_001589"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="ACHGNS_001589"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFN6937535.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="ACHGNS_001590"
     CDS             21905..22483
                     /locus_tag="ACHGNS_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN6937536.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="ACHGNS_001591"
     CDS             22535..23017
                     /locus_tag="ACHGNS_001591"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFN6937537.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="ACHGNS_001592"
     CDS             23134..23508
                     /locus_tag="ACHGNS_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937538.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            23938..>24426
                     /locus_tag="ACHGNS_001593"
     CDS             23938..>24426
                     /locus_tag="ACHGNS_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFN6937539.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMD"
BASE COUNT         8007 a         4390 c         5213 g         6816 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaca
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcaccgggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggtat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaacaa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagttttt gagagtataa aatattttgt gtaaatgaaa aaatccatac aaaaaaggaa
    23881 gtcccttctg tagaataaag ttaacgacaa ccaattcaca gaaaagagga cttccctatg
    23941 aatgatttta ctacagaaat tgtgcaaact ctagtcacta aaggcgattt aaatgaatta
    24001 ttccgttcgc acttagaaaa agcgataaac acactcctac ggactgaatt aacggctttt
    24061 ttagattacg aaaaatatga tcgcactggt tttaattcag gtaattcgag aaacggttct
    24121 tactttcgat caatcaaaac cgaatatggt gaattaacat tggaaatacc tagagatcgt
    24181 aatggtgagt ttaaacaaca aactttacca gcctacaaaa gaacaaacga tacattggaa
    24241 accactatta tccatttatt cgaaaaaggt gttacgatgt ctgaaattgc tgatttgatc
    24301 gaaaaaatgt acggtcatca ctatactcca caaaccatgt ccaacatgac taaagttctg
    24361 actgaagaag taaatgcctt taaatccaga gccttaaatg ataagtatgt cgctattttt
    24421 atggac
//
LOCUS       DAZBTF010000037        24260 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000037 DAZBTF010000000
VERSION     DAZBTF010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.037"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(44..1339)
                     /gene="purB"
                     /locus_tag="ACHGNS_001594"
     CDS             complement(44..1339)
                     /gene="purB"
                     /locus_tag="ACHGNS_001594"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFN6937540.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1352..2476)
                     /gene="purK"
                     /locus_tag="ACHGNS_001595"
     CDS             complement(1352..2476)
                     /gene="purK"
                     /locus_tag="ACHGNS_001595"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFN6937541.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2469..2957)
                     /gene="purE"
                     /locus_tag="ACHGNS_001596"
     CDS             complement(2469..2957)
                     /gene="purE"
                     /locus_tag="ACHGNS_001596"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFN6937542.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3373..4680)
                     /locus_tag="ACHGNS_001597"
     CDS             complement(3373..4680)
                     /locus_tag="ACHGNS_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFN6937543.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4683..5264)
                     /locus_tag="ACHGNS_001598"
     CDS             complement(4683..5264)
                     /locus_tag="ACHGNS_001598"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFN6937544.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5380..5477)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5836..7128)
                     /locus_tag="ACHGNS_001599"
     CDS             complement(5836..7128)
                     /locus_tag="ACHGNS_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN6937545.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7125..7748)
                     /locus_tag="ACHGNS_001600"
     CDS             complement(7125..7748)
                     /locus_tag="ACHGNS_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937546.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7751..8587)
                     /locus_tag="ACHGNS_001601"
     CDS             complement(7751..8587)
                     /locus_tag="ACHGNS_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6937547.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8604..9491)
                     /locus_tag="ACHGNS_001602"
     CDS             complement(8604..9491)
                     /locus_tag="ACHGNS_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6937548.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9507..10790)
                     /locus_tag="ACHGNS_001603"
     CDS             complement(9507..10790)
                     /locus_tag="ACHGNS_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN6937549.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11473..12156)
                     /locus_tag="ACHGNS_001604"
     CDS             complement(11473..12156)
                     /locus_tag="ACHGNS_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFN6937550.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12174..12791)
                     /locus_tag="ACHGNS_001605"
     CDS             complement(12174..12791)
                     /locus_tag="ACHGNS_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFN6937551.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12801..13625)
                     /locus_tag="ACHGNS_001606"
     CDS             complement(12801..13625)
                     /locus_tag="ACHGNS_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFN6937552.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13618..14178)
                     /locus_tag="ACHGNS_001607"
     CDS             complement(13618..14178)
                     /locus_tag="ACHGNS_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937553.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14352..15308
                     /locus_tag="ACHGNS_001608"
     CDS             14352..15308
                     /locus_tag="ACHGNS_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN6937554.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15613..16569)
                     /locus_tag="ACHGNS_001609"
     CDS             complement(15613..16569)
                     /locus_tag="ACHGNS_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFN6937555.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16673..17275
                     /locus_tag="ACHGNS_001610"
     CDS             16673..17275
                     /locus_tag="ACHGNS_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFN6937556.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17363..18862)
                     /locus_tag="ACHGNS_001611"
     CDS             complement(17363..18862)
                     /locus_tag="ACHGNS_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFN6937557.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18878..19402)
                     /locus_tag="ACHGNS_001612"
     CDS             complement(18878..19402)
                     /locus_tag="ACHGNS_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFN6937558.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19700..20449
                     /locus_tag="ACHGNS_001613"
     CDS             19700..20449
                     /locus_tag="ACHGNS_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFN6937559.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20440..21225
                     /locus_tag="ACHGNS_001614"
     CDS             20440..21225
                     /locus_tag="ACHGNS_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN6937560.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21194..21910
                     /locus_tag="ACHGNS_001615"
     CDS             21194..21910
                     /locus_tag="ACHGNS_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFN6937561.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22012..23031)
                     /locus_tag="ACHGNS_001616"
     CDS             complement(22012..23031)
                     /locus_tag="ACHGNS_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFN6937562.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23050..24051)
                     /locus_tag="ACHGNS_001617"
     CDS             complement(23050..24051)
                     /locus_tag="ACHGNS_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFN6937563.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         7188 a         5222 c         4225 g         7625 t
ORIGIN      
        1 ttttgctatt ctattttaat tgaattttgc aactcactct tctttattcc aaacctactc
       61 gtttgaatat cacgtcaacg tttttcaaat gatgatgata atcaaaggca tcgtctattt
      121 cttctttaga taataccgat gtgatggttg gatcttcttc taacagcggg cggaactgca
      181 cttggttgtc ccaagcgtac gcagtttttg gctgaacaag atcataagct tcttctcttg
      241 tcattccctt atcgatcaat ttcagcataa cgcgctggct ataaatcaaa ccaaatgttg
      301 cagacatatt ccgcttcatg ttttcaggaa agaccgttag atttttaacg atattgccaa
      361 aacgatttaa catgtaattc aataaaatcg tggtatcagg caagatgatt cgttctgctg
      421 aagaatgaga aatatctctt tcatgccata atgtgacatt ttcataggca gtgaccatgt
      481 gtccgcgcac cactctggct agaccagcca tattttctga accgatcgga ttccttttgt
      541 gcggcatagc agaagaacct ttttgacctt tcgcaaaaaa ttcttctact tcacgtgttt
      601 cggatttttg caatccgcgt atctctgttg caaaacgttc aatacttgta gcaataagcg
      661 ccatcgctga gaaatattca gcatgtagat cccgagggag aacttgagta gagatttctt
      721 gtggacggat ccccaattgt ttacatacat actcttcaat aaatggcggg atatttgcga
      781 acgttcccac tgctcctgag atttttcctg cttctactcc ttttgccgca tgttcaaatc
      841 gttcgatgtt ccgtttcatt tctgaatacc atgtggcaag tttcaatcca aaagtagttg
      901 gttcagcatg gacaccgtgt gtccgaccca tcatcactgt atatttgtgt tcttttgctt
      961 tttcgccaat gatttccgta aaacgtttca agtcttcccg taaaatatca tttgcttgct
     1021 tgatgagata gccataagca gtatctacga catccgtact tgttagacca taatggaccc
     1081 attttctttc ttctcccaat gtttcagaaa ccgctcgtgt aaacgcaacg acatcatggc
     1141 gtgttaattt ttcgatttcc aagatccgat cgatatcaaa tgatgcatgc tcacgaattt
     1201 ttttcacatc ttcttgcggg atttcaccaa gttctgccca agcttcatct gctaaaatct
     1261 cgacttcgag ccacgcacgg tatttgttct cttccgtcca gatattcccc atttcagggc
     1321 gtgtataacg ttctaacatg ttttttctcc tctaatccca aatattcgtt tgtttgattt
     1381 cttccagcgt ttgctggatg tcttctgtca agatagtgat atgtcccatc ttgcgacctt
     1441 tttttgcttg tttttttcca tagaaatgga attgccagtc tggcttattc tcaatcaagc
     1501 gatagctttc cagcagttgt tctcccaaaa cattgaccat tactgcattg cttatcaaac
     1561 gtactttttc tcctaatggc caaccacaaa tacctcgaat atgggcatcg aactgactca
     1621 tagaacaagc ttcaatagaa taatgtccag aattgtgagg acgaggagct aattcattca
     1681 catatagccc gcctgttttt gttaaaaaca tttctacccc taaaacacct gaaaggctca
     1741 ctgcttctgc aatcacacgg gcaatacgct cagcctcctc gatcacatcc tgatcaatcg
     1801 cggctggagc aatcgtttca tgaagaatat tgttgtggtg gatgttttcc acgaccggaa
     1861 aagtcgtgaa atctccttga ccatttcctg ccaccatgat cgaaatctct ttttcaaacg
     1921 ggatccatgc ttctaaaaca caggttcctt ctcttaaaag gtccatggca ggagctagat
     1981 ccgcccgact tttcaaaaca tactgcccct tgccatcata gccgcctcga gtggttttca
     2041 aaacacaagg atagccgatc ccgtcaatcg catcttggat atccgtcgga ctgacgatcg
     2101 ttgcataagg agcaatcacg atgttattcg tttccaaaaa agatttttct aacaggcggt
     2161 cttgcgtaat cgcaagcaag tctgtccctt gaggaataaa tgacatgggt aaaatcgcat
     2221 ttaacgcctc tacactgacg ttttcgaatt cataggtaat gacatctgtt cttctggcaa
     2281 gctctttcag cgcaaaggtg tcatcataga cactttcaat gtgccaatct gcaacttgtg
     2341 cggctggaca atcgctcaca ggatccagca cacctacttt aaagcccatt tcttttgcac
     2401 taatcgccat catacgacct aattgaccgc caccgacgat cccgatcgtt gaaccaggca
     2461 acaaaggttt aaccaagttg atcactactt tctataacag ttttagccaa cattgaacgt
     2521 ctttcagcca atttagcctc caattctaaa tcatacatgg aaagcatctg tatcgcaagt
     2581 agtccagcat taattgctcc cgcttttccg attgctgtag tagctacagg tactcctcca
     2641 ggcatttgga caatcgaaag aagcgaatcc aaaccattta atgttcgtga ttggaccggt
     2701 acaccgataa ccggcaaagt tgtttttgat gcgaccatac ccggcagatg cgcggctccc
     2761 cctgctcctg caataatgat tttgattcct ttttttcgag cgttttgtgc atattcaaac
     2821 atcaaatctg gggttcgatg agcagaaacg accttttttt cataagacac accgaattca
     2881 tccaatattt gacatgcttc tttcattgtt tcccaatctg aagtactccc cataatcaca
     2941 gagatttttg ctgtcattgt ttttcctccc agtttttcta cattcatatt ttaacaatca
     3001 ctttttttga tgtcaaagaa atttcgaatg ttaacaaaga aaaacatttt attgttcgtg
     3061 tttattaaag ataaaaaaaa ggaatgtgac aaaagttagg tcacattcct ttttatggat
     3121 aaacggtgtt ctcctgccaa ctcctcggtc acaaggaaca atattcaaca aacatgagaa
     3181 gctttttttt gaaattgttc cttgtgactc ggagatgaac ggcaggctca cgacctcttc
     3241 cttcaacggt gttcaatcag ctgaccctcg gtattagctc aaacaaacgg caaaatacga
     3301 ggaactattt ttccgtttgt ttctctaaca atcaggtcaa aacgctgatt tcacaacctt
     3361 tcttttgctc tattacttct tgaaggcttc ttccaatatt tcttcatctt ttttcaatcc
     3421 attaaagatc aaattcaata tgatagccgt aatgctactc attacgatgc catttccagt
     3481 gaacatttgc aatgtttgcg gcatttgact gaataatgtc ggcgtaacat tgaaacccag
     3541 cccaaatcca acagaaacag caacaatcaa tagattttta tcattgctga aatcaacttc
     3601 caacagcata cggattcctt ggactgccac catcccgaac atgactaaca ttcctccacc
     3661 aagtaccggc tcagggatga tttgtgctaa cgcaccgatt ttagggaata atcctaaaac
     3721 aatcaagaag aatgcagaga aatagatggg ttttcttgtt ttgattcccg aaagttgaac
     3781 caatccgaca ttttgagaaa agccagtata agggaatgta ttgaaaatac cgcctaagat
     3841 aacggcaagt ccttctgcac gatatccctt tttcaattct tcttctccaa catttttccc
     3901 agtaatgtca ccaagtgcaa aatatacacc tgttgattct accatgctga ccatggaaat
     3961 gatgatcatt agtaaaatcg accatacgtc gaaagttggt ttaccaaaat aaaagggttg
     4021 tggaacatgg aacaacggcg catctccaat cgcgctcaga tcaatcattc ccatgaaagc
     4081 agctaagatg cttcctccga ctaaaccaat caataccgcg attgaacgaa tgaacccttt
     4141 tgccagcatt tgcacgccta caattaggag aatcgtcaca aatgcaagta gcagatttgt
     4201 tttgtcacca aaatcagctg ctgttgcatt acctccaccc attttctcga ttgctacagg
     4261 aatcaaagtc aaaccaataa cggtgataac cgttcctgtt actataggag ggaatagttt
     4321 tttaatttta gaaaagacac cagaaactaa cacaacaaag atcccagaag cgataataga
     4381 tccgtagata gctcctactc cttcattggt tccgatcaag atcagtggag ctactgcttg
     4441 gatcgcacat cctaaaacga caggcaagcc aattccgaaa aatcggttaa ctgtcaattg
     4501 caataatgtc gctaatccac acataaagat atcaattgaa atcaaataag tcatctgttc
     4561 ttgattaaag cctagtcctg taccgatcaa aagaggcacc gctactgctc ctgcatacat
     4621 cgctagcaga tgctgcagcc ctaaaaccgc agcttttcca ttttgtgttt ctttttccac
     4681 gattatgcgt cctcctcaag aaactcaact tgtccattcg tcaaggaagc aatacgcgca
     4741 agagaaacga ctctcaatcc catttcttca agtaactgac gtccatcttg gaaagacttt
     4801 tcgatgacga tcccgattcc ttctacttgc gctccagctt gctggcataa ttcgatcaat
     4861 ccttttgccg cttgtccgtt tgccaagaaa tcgtcgataa tcaatacttt gtcttctgaa
     4921 gataaaaatt tacgagaaat agaaatcgta cttgtcactt gtttcgtaaa agaataaaca
     4981 gaagctgtca ataattcttc atccatcgtc aaactttttg cttttctagc gaagatcatt
     5041 ggaacatcta attctcttgc agcaaataaa gctggtgcga tcccagaagc ttcaatggtc
     5101 acaactttcg tgattccttg ttctttaaat acttctgcaa accgttttcc catttgttcc
     5161 atcaatacag ggtctacttg gtgagtgacg aagctgtcga cttttaatac gccctcgcct
     5221 aatatacgtc cgtctttttg aatacgttca actaattctt tcactttatt tgctccttta
     5281 tcatttatta ataccgaaat aaccgctttt gtgcaaaaac ctactatgtt tctgttaaag
     5341 aaaaacaccc tccactttat cataagaaaa gaagggtgaa atagccaatc ttttacttat
     5401 agtccagcat ttacggtact ggctagaaac tgtcatccat attatgacga tatataagta
     5461 ggtatgtaaa actaataata gtgtaaattc attttcttgc aactgttttg cacgtgaagg
     5521 cagtttgcca tatttgaata aaaaatgttg tttcaaatat tgaaaacagt ctaacactac
     5581 tttctgaaac tgacaacgtg gtttacttat ttttttgtaa aatctaatga tttttaatgc
     5641 attattcctt tttgttcggg tttaaatagt tatttttatt ttctcctatc taaaaaagca
     5701 gcccacataa aaagaaggca tgacaaaaag catgtcatag cccctttttc cgaataaata
     5761 gtgttcaaaa gacagcctat cggcaataag gcaagatttt ataaactacg ggaagtaatt
     5821 tccgtaaatc tattcttatt cttttaagct gacaagctcg tcaatcacta ctgcttttcc
     5881 tgaagcaata gaatgatttg ctgcgattcc tgtcaaaatc gacaaaatac catctacgtg
     5941 agttgctgcc cgattccacg gatcttcttc tttttcttca ccaaatagat catctaataa
     6001 acgcggatct cctccgccat gtccgcccgc ttcttgttcg acgattacct catatggctc
     6061 accgaacatc ggatagacat ggatggactg aagaacggct gctccttcat cctttttatc
     6121 tccacctgca ttgatgtaag acttttcgat cactttcatt tcgatccgtc ctttgctgcc
     6181 attgaaagaa acattgaatc cttcccacgg catatacgcg tttagcgaat acgtcaaaat
     6241 cgcaccagtt tcatatttga ccgttacgcc tagtgtatct tcaatagaga tatcatcgga
     6301 aaatacactg cggtcacgaa tatatccact gtctttttct gcttctaaat acatttctcg
     6361 caattcctca ttggctgaca ggtctatagc aaatggatca gctttagcta gttcgcttcc
     6421 atagcatcga ctgtaaaata tttctactcc gcgtttctcc gcattatgca tcccataaaa
     6481 gtttaatcca ccgaatgcaa ataccttttg gggacggctg ttcaaccaaa aattcactag
     6541 atcaaaatga tgggtagatt tatgaacaag aagcccgccg ctattgtgct tatttcgatg
     6601 ccatcttctg aaataatctg ccccgtgttg cgtatttaac agccattcga agtggataga
     6661 aaacacatca ccaatcacat cactttctat cagttccttt atcttcgtgt gatacggtgc
     6721 ataacgataa ttaaaagtga ctcgcaggct tcttcccgtc tgtttttgga ccgttattat
     6781 ttcttgagct ttttcctcat cgatagtcag aggtttttcg caaataacat catatccttt
     6841 tttcattgca cgaatgatat aatcatgatg cgttcgatct actgacgtaa taatgacttc
     6901 agccggccgt acgtcatcca gtagttgctc aaattgtgat ggcatacatg tctttacttt
     6961 tttctgtcca tacttgttca caagtatccg atttgcataa tccatccttg tttgagatac
     7021 atcacaaaaa ccgactagtt cacaagtttg atgatacgta gaggcaattg cctcataaaa
     7081 aaaacgcgct ctcccaccaa gtccgacttg tacatatttt ttcttcattt aaagtcattc
     7141 tccttttctt ttaagcaaaa tactgtgaca aatggcagcc ctggatacag taatgcagtg
     7201 agtaaaaaat catgctgtat acaccagaac agtccataca aaaggcataa ataccacaga
     7261 aaccccttga cgttttttga caacagccag atattccttt tgatcaaata aacaagacta
     7321 tctgctttat cttctttgca gatatcaatt agcgtactaa aaaaactatg ggatacaaaa
     7381 agcaacagac acaatagaaa aggaatcaga taccaagctc caatctttcc cactaataaa
     7441 taccaatcaa ctgcaaaaat aatgacaaac agctgaaaca acgtaatgat gaaacacatc
     7501 cgtttgattt ttcctttcct ccaacatacc tttttttctg ccaagtaatg gacagataga
     7561 taataagaag gatacatcat caaaaaataa atcagataga caacacgagc agacagcact
     7621 aatcctaaag tgaacaaaca tcctagaact aaagcgaaaa gagcattact gataagactg
     7681 gttaaaaaaa tccgacagac atagtagttg agcttgtaaa gaaacatctc ttctattggc
     7741 tttttcatat tcagcccttc agaccgcttg tactgatccc ctggacaaga tatttattga
     7801 agaataagaa aatcagaaaa accggtagca gcgacaacgt agacatcgca aacatactcg
     7861 cgtaagtcgt actagatcca gcgtctgcaa ataatttgat ggcaatcgat accgtgtaag
     7921 atctcggctt gtttaaataa atcaatggtc ccatgaagtc gtcccatttc caataaaact
     7981 ggatgatgat cgtcgtgatg atcgaaggtt tcagcaaagg aaaaataatt ttggtaaaga
     8041 cactatactt gctgcatccg tcaataatcg ctgattcatc taactctttg gggatgctga
     8101 ccatgaattg catgatctgg taaatgaaaa atgcttgtcc aaaaaaatgt ggtaaaatca
     8161 gcggcacata cgtcccaacc aaattcagcc gattgtaaat aataaactgc ggaatcaaaa
     8221 tcacttgtgc aggaatcatc atcgttacta acatgacgac gaaccagaat ctttttcctt
     8281 tgaaatcgat tctcgataat gcgtaagcaa tacaagtcga gctcagaact gtccctatcg
     8341 tcgaaactgc agagatgaca aacgaattcc taaagaaagt aaaaaatgaa attccagcaa
     8401 accccgccca tccttttgcg taattttccc attgaaaagt ctctggaatc aaattcagcg
     8461 aaccactcat aatctcttga tccgttttaa aagaacctgc gatcatccat aagaccggat
     8521 aaatcatgac tagacctaat agcaaaataa aaccgtattg aaaccatttc gttactgtat
     8581 tattcatttc ttcctcctct cattcacaca acgacccatt tttttgatga tttgaaaatc
     8641 agaaaggtga cagctcccat aatcaacagc atgatccacg aaatagcact agcatacccc
     8701 atagaattaa aagaaaaagc ttggttatac acataaagag cataaaagtt cgtggcatca
     8761 ttcggaccac cttgggtaat gataaatgcc tgcgtaaaag tcataaacgc cgcgattgtc
     8821 tgcataacca agttatacaa aatgatcggc gaaagaagag gtaacgtgat cttgtaaaac
     8881 gtctgccatt tgttcgctcc atcgatctct gccgcttcat aatacgagcc tgggatctct
     8941 ttgatacctg cagcaaagat gatcattgcc gaaccgaatt gccaagcaga catcaaaaca
     9001 agagggatca tgaccagttt ttgatcgcta aaccaggaaa gcgcaggtgc gcctaatcgt
     9061 tccaggaaaa tattcactaa cccgttttta gaaaacaact cccgccaaac tagagcgata
     9121 gaaatactgc caccgatcaa actaggcaca taatacaaag aacgaaagaa agtcgttcct
     9181 ttgatattcc tcgttaaaag ataagcaatc aatagaccaa agatcagttt cacaggtaca
     9241 gatgcaaata catagagaaa agtgactcgg atggagttca aaaaacgggc atcactcagc
     9301 atctttatat aattatccaa gccgatccat gagactggat ttcccaaacg ataatcggtg
     9361 aacgaataat aaagtgacaa gatcatcggt atcactgtta tcaatagaaa tcctaggatg
     9421 aatggagaag caaatacgta accgaccaca ttgtcttgat ccaagaaccg ttttagtttt
     9481 tcttttttca tgccttcctc ctgctgctat cctagaatgt ttgacgcaaa ctcataaaga
     9541 ctctctgctg cttcttctgc tgttacttct tcataaatga ctttttcaac taaaagattg
     9601 tattggtctt gaatttccgg attcaaagca ctatcaaaga cattcacttc gccgttgtcc
     9661 atatcggaaa tcatttctaa ataatcaaat gtcgcttgca aagttgcatc atctgcacga
     9721 gcgagctgtt ctcgaatatt cgacatgatt ggtacaccac gttctccttt taaaatttca
     9781 ttggcttctt cactgttttg aaagaaatca ataaacttag cagcttcttc tttatgggct
     9841 gagctttttg ccatactcag catttgcgaa gaacgtaaag ggataccaga ttctccatct
     9901 gcttttctgc gaggtggatt aatcaactga atctccatat cttcaggagc ccctgacaat
     9961 atttctgtca actggttact tgatagaaac accattgccg cttctctatc cctaaccgcc
    10021 tgaaaagaca gatccgtcgt acttgcagct ccaccacctg gttctggatg ggcccctttg
    10081 tctaccagat ttttacgcag ctggaaaaaa tctgttagat agtctggatt ttcaaatccc
    10141 aagctgttag atagatcttc ccgattataa aaattcaatc ctttctcaac ttgtggtatt
    10201 tgaataatac agccagctat aaaatcttcg aatcttgaaa tagcatagat tcctaattct
    10261 tcagtgattt gatagctgat ttgttcaaat tcatcccatg tccaatttga agctggttca
    10321 gggatattta attgttcaaa tatattttta ttgtaagcaa tcgcaaacga attcaccccg
    10381 ttagacagtc caactaattg cccgtcgtaa gtcgtcgttg ctaaatagga ttcatctgca
    10441 ttagaaatat caattatccc ttgctccaca tattcatcta atggttcaat ctgattgatg
    10501 tattgaggaa attgattgtt cagttggaaa atgtcccaga tatcatctga tgctgctagt
    10561 gtacctaatt ttgtgtaata tccttcataa tcataaaatt cataatcgat cgtcacatgg
    10621 ggattttttt cttcataaag ctcgattact tttactgttg catcgtgtcg gacttgcgaa
    10681 ccccaccaag ccatgttcag cgtgattttt ttgtcatctt cttgcccaca agccgtcaag
    10741 aagaataaca taacacttaa agctaagatg attctcattc ttttcttcat tctctgcacc
    10801 tctacttcca ttgttatttt tcaatatact gtatttttat acagaacata tttattaaac
    10861 gatctttttc aaggggaata aaaaccaaga cttgccttat cgttctgcct aggatgaaca
    10921 actcaatact ttcaagaaaa attagatagt gctgattctt ctttcatcgc taaaaacact
    10981 tcttgcttca atgatgggag gataattacg caaagaaaaa aggatatcag acaactgccc
    11041 aatatccttg acatagctaa ataagcatgc ttcttcgtgt gcgctcttcc ctaaaaaaca
    11101 gaaaaataac tagtaattct gtatgaaatt aggtacaata tacacgtcga tgcacaagca
    11161 ttgactatgg caacttgatt ggtcagttcc cgctgacttt tcaagccccg acaatttagc
    11221 tgctactaaa ttgtcgggtg ccttttttag tttttattcg tttttcattc tatagaaagc
    11281 gcttcaataa atcaagacat ttttttgtta gaatgctaat aataacaaaa ggttttaact
    11341 cttcttatgt acattacagg taaaaaaaca aaaaaatttc tctttttttt gaattctgaa
    11401 caaatagtac tggagtacaa aagacgagga agagccacta tctgctcttc ctcgtctttt
    11461 gattctcacc tgttattcat gattcttttt ctcagtttcc cgatattttt ccattttacg
    11521 actaaaaagc tctccgctac ttggtggcgt atatgtgatg gcatttgctc ctgcttgaat
    11581 ggtctcagca atactttctt ccgttggacc acctgtcgca atgataggaa gttcaggata
    11641 ttttttacga aaatggcgta ctgcttctgc agtttcttta cctgcactga tattgataat
    11701 cgaaatacca gctgctaact tttcatctat ttctgtatat ttcgacgtta ccgtactgat
    11761 aactggaata tcaactactc gacaaacttc ttttactgtc tctaaaggcg taggaccgtt
    11821 caaaacgacc cctaacgaac cttgcgcttc tgcgaacatg ctcatgtagg aagaacgagc
    11881 accttgtgtc aaaccaccac ctacaccgga gaaaactgga atgtcagctg cctcaataat
    11941 gcttttagta atcgctggat gaggtgtaaa aggatacaca gcaattactg cattcgcatt
    12001 tgtattacgt atgatcgcaa tatccgtagt aaagatgatc gaacggatca attttccaaa
    12061 tatcttgatc ccgcttgctt ctaaaatgat ttccggtact cttacgatat cttttctcaa
    12121 ttccgtcatg atttctggcg taaattgttc tgccatcagc cacttctcct ttgctatctc
    12181 cggtcatact gattcaattg atacgtctcg atgacctgga tgattttttg cgcataaccg
    12241 gggtctgtcg cataacctgc atcttgcaat gcttgtgcgg cttcctgata gttggtggcc
    12301 gtaatcactt tttcatacag cgcaggattc caatctactc cttggacgaa cagttgtgta
    12361 tggtcatcca tggactgttc ccatgaatca tacactttga agtctccttg gatagttatc
    12421 cattcttcgt taacaaattc ctttgtttcc aggcttactt tcttctgatc cccataagcc
    12481 ttgatcccga aaagattatt gtatttgcta gccaaggtac tttttcccca atttgattct
    12541 aatatagctt gtcctaaaat gatactagga agcactccat aaccatcttg tagttcttgg
    12601 gcatgcggaa caagccgttc aataaattgc tgatgggatt gcgcttcatt ttcttgctga
    12661 ttatcagtaa aaggagaaga cagattcttc aaggaaaaaa caaaggctaa gagtatcact
    12721 gctacaccgg caaccactgc tggccaacgg atattcttgc gcttttgctt tttccacttt
    12781 tttttcgcca tgatcaattc ctacttttca tttaattcat tcagatattc ttctagcgtc
    12841 aattggtgtt tggtgatata ctcagcgttt tctttaccga cataacgtaa gtgccaaggt
    12901 tcatatgtga tcttagttat atcttcgtgt ccttttggat aacgaacgat aaatccatac
    12961 tgcgcagcat tatctgcgat ccatttagct ccaggctgat ctccgtaact tgcatccaat
    13021 acagtagaag gataactgtt gtaccagttt tgatcgacga catcgactgc cagacctgtg
    13081 tggtgttcgc tgtatcctgg ctccgtaatc gtttctttag taatctttgt cgcctcctct
    13141 tctgatacac cttgacgact catcacatct tggacatttt gttcgaaaac ttgttgttgg
    13201 gatgatacag accggtaagc tgagaccatc actaatggaa atccagctgc ttcagcagcc
    13261 ttggcaaatg cttcatattc tggagcaatg cgtttgtcga tcaagtatcc gttacttaat
    13321 gatgccaatt gagtagactc atcaatttct ttttctaatt tgtgatctgg accaaccaaa
    13381 actagcttcc aatcgtctgt tttaacatct ggcaattgtt tggacgtacg atcttttgtt
    13441 tctttgtcct ttaccttgct gctggttgtt tgactgttct tttctttact agcagttgtt
    13501 ttggtgcttg ttcgttcgac tgcttgattg ccggcttgaa catgcgaatc atcatcgcta
    13561 aaaataacat agcccattgc tgcgatcata atcacaactg ctccaaatcc taaaaattta
    13621 ttcacttgaa aaatcctttc gttgttcttc acgttcccca ttcgcctcat gaacgtaagt
    13681 ttcttccaga ttttcattca cccaatcgag aagttcgatt ttttctcctt cgatctgttt
    13741 ttgaacatct gctggcagac gaaaattcaa aatatcatca tagccccatt catcatattg
    13801 aatacttact ttcaagtcaa ggtatctacc agcctgctga ttatttttca aagggatcag
    13861 ctcgactttt gctgctttgc tgctaagcca tttttgcgcg atgcgtgttt tgatttgctt
    13921 gtactctgca acggcaacgt ttcgtttttc gggaaaaata atcgtttgac gcaaaatctt
    13981 ttgcgttagc atccattcat aatcagtgaa aagatttttt actttctgca tcgcttcaga
    14041 tggcagattt ttctcgccat ctttccattt ttgccattgt tctggactga tcagcaagta
    14101 ttcatctaaa aaattttttt cagtttcaaa ctgttctaat aatacactca taataagatc
    14161 aatatatact tgatgcacgt ttatcccctc cttttaacta ttctacttaa gttttgatca
    14221 aaaagcacct cttaaatcgc tgctttcatt ttttttaatg tttcaagtcc tctcctcttt
    14281 attcttcgtg atttttgtta taataataga cagacaagaa actcaatcca actatgaagg
    14341 agcgttttca gatgaaaaaa tatcaatggg gaatcgttgg tcttggtacc atcgcacatg
    14401 agtttgccga aagcttcaat caagagacga gcgaactaac agcagttgct tccagaactt
    14461 cagaaaaagc ggaaaacttc gctcaccgtt acaatattcc aaaagcttac ggaagctatc
    14521 aagaaatgtt agacgatgct gaaatcgaca tcgtgtacat tgctgtacca aatagacagc
    14581 atatcgacca tatcttggca gcattggaag caggcaaaca cgttctatgt gaaaaagcca
    14641 tcacgatgaa taaaaaagaa ctggctgacg ctatgcgctt agcagaagaa aagaatttga
    14701 tcttagcaga agcgatgaca atcttcaaca tgccacttta tcagcagtta cgctcaataa
    14761 tggatacagg aaaactgggg gctttaaaaa tgatccaagc acctttcggc agttacaaag
    14821 aaccagaccc taaaaaccgg ttcttcaatc cagaattagc tggaggagct ctgttggata
    14881 tcggtacgta tgctgtatct ttcgctcgtt tctttttgag ctctcaacct gaagtagttg
    14941 cctctacaat ggttcctttt gaaacaggcg tggacgaaca atccgtcact atcttgcgta
    15001 acaaagaaaa tgaacttgct gctgtcagct tgacttttca ggcaaaaatg ccaaaagtcg
    15061 gtatcgttgc ctttgagaac ggttatgtga cgattgctga ttacccgcgt gctgatcgtg
    15121 cggagatctt atttacagat ggtacaaagg aattcatcga aagcgggaat acatctgaag
    15181 caatgaatta cgaaataaag aatatggtac acatgattga aggtgacttg cccaatcgct
    15241 ctctcttctt gacaaaagat gtcatcgaaa tcttggatca aatgcaagtt ctatggaaaa
    15301 atatgtaatt ttaaaaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    15361 acaaaaatag ctcctcctat ttttcgtttg gctggactta ttaccgcaga ggaaagcttt
    15421 tgaacaccgt ttaagaaaga ggttgtgagc ctaccgttca gctccgagtc acaaggagca
    15481 atttcaaaaa acagcttctc atgtttgttg aatattgtga cttgtgaccg aggagttgac
    15541 aggagaacac cgtttaaaga agaggttgtg aaatcagctt tttgacctga atattagctg
    15601 aattcacaac ctctattttt ggattttttg ttgggagaat tctgccttga tttcaataaa
    15661 acttttaaaa gactgcaaaa actggaacaa ttctgctcga tcctcgaatt cagggcgact
    15721 agtcggcaga ggcatttgcc gatacaatac ataaacttct tcaatacgtg ttaaaatttg
    15781 tctgccatca tttgactctg aaaatgtttc tgcagtataa atcaataaac catagatatg
    15841 ttttccataa taagctggtt gctggattct ctctagattt tccagcatgt ctttaatcac
    15901 attggcctgt gctcttctca tagagaagta agtttcgtag tatagcggct gtctgagcca
    15961 gtagttttct tgatgttctc tggcatcttc ttggctttcg cgaatgaatg tcagtaaatg
    16021 ttcgcatttg atctgcacat cttctttatt ttctgataaa agaaattctg ccatttgtct
    16081 caaaatattt cgaaattctt tttcgatctg tatttggttt tccttcagct gcttcgtacg
    16141 atctggcatg taaacatttg caagcaaagc aaatactaat ccaatcgcca ttaatcccgc
    16201 ttcattgccg atcagctgcc aagacatgtt ttcctccact aaataatgag tgaccaaaac
    16261 cgaatttact actatccctt cggttaattt gaagcgagag gcaagcggga tgaacaggaa
    16321 taaaaaaaga ctgaagccaa aaatagtata cccaacagca aaaaaactga acagtgctgt
    16381 tgccgtagct aatataaagg ctgcgattcg attcatgccc gtacgcaatg tagagcgctt
    16441 ggtatttcct acacttaata atgcaatgat ccctgcagct ggccaataca acagcgataa
    16501 gctacccgct gtgaccatcg aaaagaagac actgatcact gttttgactg ttcgcattcc
    16561 tatacgcatt tacttccccc ttttttattt taattatact tgaataatga aaaactagct
    16621 atcagacaaa agtttaggta tgatagtaaa aagaccaaaa aggagaatct ctatgcaatt
    16681 tggccgtttt cgactaggta tgcgtacgat caaaagtgcc ttagctgttt ttttatgtat
    16741 cttgtttttt catttgactc aacgaggaat cccgatgatt gctgcattat ctgctgtttt
    16801 ttctttgcgt caggacttga ctacaaccgt ttcttttgga aagtctagga tcatcggcaa
    16861 tacattaggt ggtacattag ctatcattta tttttttgtc aaaagctatt ttcacaatga
    16921 ttttttggta gaattgatct tgcttcctat tctagttatt attgtgatcg tcgtgtcaga
    16981 tggtattaac aataattccg gtatcatttc cgcaatagct acgttgttgc tgattgcgtt
    17041 gagcatccct caaggcgaat ccgctttgta tgctatccaa cgtgtactag atacttttat
    17101 agggacattc attgcaatcg gtatcaattt ctttcttcgt cctcctgaaa cagaaaagaa
    17161 ggaagaacta tcagaagatt tggtcgaact ccaaaagaaa gaacgattgc taagaacaaa
    17221 tttacaagag atagaagcta agatccaaaa acaaaagaaa aatagcgagg actaattttc
    17281 ttttgcaata aaataaaaaa atggagagaa gaactttaaa aagtcttcct ccatttttga
    17341 ttgaacggtg aagtgtacta ctttattctt ccttcatcga gttttgttct gcctcttttg
    17401 gcgcttcata catatttact tggctagtag tcccacgtgt cgacaaattc tctacgattt
    17461 ccttcaagtc aagtcctgtt gtcgctttta gtgtttcttg agtagaagaa agcaaatttg
    17521 tggcataatt tgtcactcgg ttcgctccac tggtttctcc tgaactgctt cctgtatcga
    17581 caacagagat tttttcgata tttcctagcg gctgtgctgc ttcgcgcatc aattgcggta
    17641 gcatatcgat aaccatactc aagacagctg cttcgccata ttccttgaag gcattggcga
    17701 tcttttgttt tgcttcggct tctgcttttc cttttgctag tgctgcttcg gcttctgctt
    17761 gtccagctaa acgtgtctta ttggcttccg cttctgccag tgcttccaca cggaatcgtt
    17821 ctgcttcggc ttcagtcact tctttgactt tttgagcttg tgcttcttgt tctttagcgt
    17881 accgatcggc atctgctttt ttcttcactt ccgaatcata ttgcttttca cgacgtgtga
    17941 tttctttttc ttccagttcg atttgttttt gacgttctac gattttaatc tgcatttcct
    18001 gttcgattac ttgttgttgt gctcgggcac tttctaaatt gtaggcttgg tctgcttttg
    18061 ctttagcaac atcttgttct tgtttgtagg ttgctaattt cagttctttt tcttttaatg
    18121 attctgcgat ctctgtttgt cgctgtaatt cagctgcttg tgattctttt tctgcttgag
    18181 cttttttgat acgcgtttct tttagagctt ccgcttctgc tatatctgca tctcttttga
    18241 cttgggcgat tcttggtttc cctaaagagt caagataacc gtttttgtct ctgacctctt
    18301 tgatagtgaa agaaacgatg atcaacccca tttttgcaag gtctacagaa gcgacttctt
    18361 gtacgctttg actgaactta tcgcgatttt gatagatttc ttctacagtc atagaaccaa
    18421 gaatcgagcg caagtgtcct tctaatactt ctcttgcttc attttccagt tcttctcttg
    18481 tcttgcctaa aaactgttct gctgctgtag caatctcttc tacagatgaa ccgattttga
    18541 tgatcgaagt accatcacac atcaccggta cgccttgttc cgtatacact tcaggggtgg
    18601 agacgtccaa tttactcgat aatagactga tccgattcga acgttgaaaa actggcaaga
    18661 cgaatgcgcc acctccacgc acaattttga ttttgttatt gctttcatca atgtgcacat
    18721 ttttgcttcc taaataactt ccactgatga tcaacgcttc atccggtttt gctgtctggt
    18781 acttcgctac aaagacgatc agcaacatca gtaaaatcaa agctgcgatt gcgatcgcga
    18841 taaaaacaac tggtatttcc atttaacaca cgctccttta agcttttatc atgctttcat
    18901 aaggcatgac ataacagatt ctttccttga tctcaatgat caatactttc gttccccgct
    18961 cagctgtctg ttcttggggg ctgtaaaaac aagctggacg actaatggaa ccggtaacac
    19021 ttttcaactg aatttctccc atacctcgta cagggatcgg tgtgatcaca acagctactt
    19081 gtccttccag agttttttca gaagacgaca tcgttgattc ggcatgtttc atcggcatca
    19141 atatatagaa attaagcaaa aataccaaca ctatcgatat gcctccacta gcaataaata
    19201 aaaataaaga tgattgatct gttaaacgct cacctagaaa accaaataag cttgtgaaag
    19261 ttaaccaagg gataataagc atcggatcaa gcgggccatc aaaatcgaat acatctccaa
    19321 aaataatcaa taggactgcc aatcctgcac aaatcagcaa tgtataccaa taaatcgtct
    19381 caagtgcgat tccccctatc atatacacgc ctccttccat ttctacgtct atcataccaa
    19441 tgtttgaaaa ttctgtatag cttcttttac cgactcagtg gtgtttttta taggaaggtt
    19501 gtgtttatcg ttgacaattt gttaaatttt tgttttcatg ttaaaatttt cacaataaat
    19561 tctttcgagg tagcctatgt ttcctttttt taagaaaaaa ccattaaagc tccagaacac
    19621 gatcatcctt gtcgtttgtt tctccacatt cgtttctttg attgtttctg ccatattgat
    19681 caggaatttt attctttcga tgaaccgatc tagtgtgtca gataaaatct ctagcattgc
    19741 taaaatcgtt gccaatgata aactcatcca aaaaaagctg cagactggtg acccgacaca
    19801 tgcgattcaa ggaataacaa atgaaatcat gattgcatcg aatttagatt ttatcgttgt
    19861 catggaccct gaaggcattc gatattctca cgcagaccca agttacatcg gtcttccttt
    19921 ctcaagtcat gaagatgtga aaaaagtgat tggaggacaa gaatttttct ctgaaaaaga
    19981 aggaatgtta gggttcggct tccgctattt cgtccctatt tattatgatc aagagattat
    20041 tggtgcagtt tgtgttggct taacaaacaa cacgattgac aatcaagtca aagatggaca
    20101 aaaaatgatt gttttttcat tgattattgg acttagcgta ggcatcatag gcgccgtttt
    20161 tcttgcccaa aaaatgaaaa aagtactgct tggacttgaa ccacaagaaa tagcagaaca
    20221 gttaagtgaa aaaacgatta tcgaaaatga agtagatgaa ggaatcttag ccatctcttc
    20281 tgataaaact atcctcttga tcaatacacc tgcaaaaaac attttctacc aaacagagca
    20341 gctattggat ttcgaagtag gaaaaaaatc agtgataatc tatatgacgt tttgtttaaa
    20401 gagacgattg agcatcaaac gaccgttcgg actcaatcca tgtatttaaa tgggatagaa
    20461 actatttcta gcgttacacc tctttttagc aatgaccgtt ttttaggagc agtcgcaact
    20521 ttacgaaatc gatcagaagt aatgaaatta atggaggagc ttggggggac gaaacaatat
    20581 ataaactcca tgagagctca aacccatgaa ttcatgaata aacttcatgt catttctggt
    20641 ttgattgatt tgaagaagta tgaagaagtc acccatttta tccagcagct aaatcaaaat
    20701 tataaaaaag agattggcca agtctcaagt ctgatcaaag tacctgcaat tgccggtttt
    20761 ttattaggca aaataaacga agccaaagaa caatctgttg aaatgctgtt agaaagcgat
    20821 tcttttatcc ctaaaatgga gatggatgac tccatccatt cacttttgca agtgttaggg
    20881 aacttacttg ataatgcaaa agaagccgtg ctacaaaaac atgaaagaaa cggacaggtc
    20941 cggattcatt taaactatga cacagaagct gaagtattca tcgtgaccgt ctgcgatgac
    21001 ggtgtcggga tatcagaaga aatctcagaa aaaatattta cgctaggttt ttcgacgaag
    21061 ggaatcaatc gtgggtttgg attgaatctc atccattcaa tcgtcaaacg ccacggtgga
    21121 tttttagaat tttccaaaca aacaaatggc ggtacaatcg tctatctaga attaccaaaa
    21181 aaggaagatc aaaatgatga caaatgtcct gataatcgaa gatgatccaa tggttgcaga
    21241 attgaatcgt acttttgtgg aaagcatccc taatatgaac gtaatcgaaa gtttcagaaa
    21301 tgtagaagaa ggaaaaaaat atttcagaca aaaaaacgca gatctgctgc tgcttgatgt
    21361 atacatgcct ggcgaaagtg gtatccattt tttatcttgg cttagacagc aaggaaatga
    21421 tatcccagtg attttgatca ctgctgcaga tgatatcgaa accataaaga atgtgatccg
    21481 ctacggtgcc ttagattatt tgatcaaacc gttcactttg caacgttttc agctggcgat
    21541 cgaaaagtat cttgctttct ctcagacaat ccaaacgact gaaaaagccg atcagaaatt
    21601 gatcgatcaa gtattcaacc acaccactcc cattcacgaa ataccatcat tcatcgattt
    21661 gcctaaaggt ctgtcaaaac tgactttaaa aaaaataagt gcatttattc taaaagaaga
    21721 tactattttt tcaacaaaag aactggctca aaaaagctca ctgtcacgta tatccgtcaa
    21781 gaaatatttg aattttttag tagaacaaga tatattgaac gaaagaatca aatatttgga
    21841 aatcggccgg ccgctaacct gttatgaatt gacaaagaat gggcggaaaa agctcacaga
    21901 ttactgttga gtcatcgaat tatcccatac aaaatagtcc aagtatacag aagtaacaac
    21961 tgtttacttg gactattttt aaacatttta ttgacgaata tccatgtttt cttatcttaa
    22021 ttgacgggct ttaggaagag atacctcatt tttcgttttt ccagtttcta ttatttcacg
    22081 aaagttctcg aaacttgtcg aaatcatatt tgataatgcc tcatctgtat ttgaacctac
    22141 atgcggggtt acaagaactc tagggtacat ttctactaat tgctggatag cagggtctgg
    22201 aatcgtctcc catggcttga aggatcggaa aaaaacctct gtttcatttg caaaaacatc
    22261 ggttgcaaaa ccagctaaat gattgctttt caaagctctt aaaatagctt cattatcttg
    22321 aagctctcca cgggctgtat taatcaagat gctacctttt ttcatttgag ctaaaaatcc
    22381 ttcattgatc atttgatcgt tttcacctgg gatatgaggt acatgaatac tgacaatatc
    22441 gcttttggct aataactctt caagcgagtg gaacgtcaat acttttttag cagcttctgt
    22501 ctgatgaata tcgtagccga tcactttggc tcctagtccc ttaaacaaaa cagcttccgt
    22561 caaccctatc ctgcctgttc ctatgatccc tactgtgcaa tttctgattt cacgactaaa
    22621 catttgctca tccactcgaa aatctttgaa agatgttcgg atcgtcgtat acgcagtatt
    22681 tctcaaaagc atcatggcaa aagtcaaaga taactctgct atcgcatttg gcgaatacga
    22741 tggtacacgg gctactgtca taccaaaatc agctgccgct tgtagatcga tatgattgac
    22801 tcccacagtc cgtgtaaaaa cataacgaat cccatattcc tgcatttttg caagattggt
    22861 ctcatctgct atacaatttc ccctcaacaa tacagcatca tggtcctttg ctgacacgat
    22921 attgtcatgt gtcaacagtt cttcataaag actgagatca taatggtaaa tattcaattt
    22981 ttcaaaaaaa tctatctcgt tcggacgaac accgtaacat gcgattctca tactttcttc
    23041 ctccatttct tatgaaaata aacctatgcc atgaatcatt tgtaatacaa cgatccatac
    23101 aggtgtcagt accacagcaa aaatagttga cagtaaggat gcattggagg ctaaaagtgc
    23161 ctccctatca aaactgatag cataggctgc tgccactgtt gcagctggtg tagccatcat
    23221 gataacgatt gttgccagtg cttcaaaatt gactggtaga atattcgttg ccgtcaaaac
    23281 tgctagaatc acgatattca atgcaggtac aaataccact ttgactagac tgtaatacca
    23341 tgaagttttt tcagatgcag cttttttgaa gcttacttca cctaatgttg cgccgattgc
    23401 tagccacgcc aatggtgaac ttaaaccagc taaataatcc atcggctgaa atagccacgg
    23461 tgcagtctgg tcaattcgca aaaacgctac ttggcttatc tctccttctg cacctgtaac
    23521 tgacacttgt ggcagatacc cttggaacag ccaaatgaaa aggccggcaa aagtagcgat
    23581 aatgattgga ttgacgaaca tcgctttgat atttttagct tccatcttca agccactcat
    23641 ttttatgtat ccataagaat acaaaaagat tcggtagcca atattgaaaa ttgaggaata
    23701 catcacgccg acaggaccat aaatcgcact gacgataggc gttccgaaaa aggtcgtaga
    23761 accaaagatc gtcaggacac gcaacgttgt ttgcttatcc cctttatagc ttaagaaaaa
    23821 aggttttgaa actagaataa gaataatata aataactaat ccccaaatca agacattgat
    23881 tccttgcatc agtaattctg tattgatatc ttgcatgaaa gctcgaaatg ccagtgctgg
    23941 caaagccact gttaagacga ctttcgatag aatttttcca acagattcag aaaaaatccc
    24001 cttttttcta caaacaaacc ctaataatat aataaaaact gttgaagtga ttgcactgac
    24061 aatcgacata tctgtcaaag tcgatcgaaa aatgtcaaat aagttcatct ttctcctcct
    24121 aattatgttc atttattcac aataaaaaaa gcttttattt tgctaatacg tattaacatt
    24181 tttaatttta tctttacatt gttacttatt gaacaatagg aaattaaaga aacttttgaa
    24241 acttatgtaa agtgattttt
//
LOCUS       DAZBTF010000038        24204 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000038 DAZBTF010000000
VERSION     DAZBTF010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24204
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.038"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..890
                     /gene="purR"
                     /locus_tag="ACHGNS_001618"
     CDS             72..890
                     /gene="purR"
                     /locus_tag="ACHGNS_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFN6937564.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1393..2766
                     /gene="glmU"
                     /locus_tag="ACHGNS_001619"
     CDS             1393..2766
                     /gene="glmU"
                     /locus_tag="ACHGNS_001619"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFN6937565.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2860..3831
                     /locus_tag="ACHGNS_001620"
     CDS             2860..3831
                     /locus_tag="ACHGNS_001620"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFN6937566.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3837..3939
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4005..4574
                     /gene="thiT"
                     /locus_tag="ACHGNS_001621"
     CDS             4005..4574
                     /gene="thiT"
                     /locus_tag="ACHGNS_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFN6937567.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4655..5428)
                     /locus_tag="ACHGNS_001622"
     CDS             complement(4655..5428)
                     /locus_tag="ACHGNS_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937568.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            5695..7395
                     /locus_tag="ACHGNS_001623"
     CDS             5695..7395
                     /locus_tag="ACHGNS_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFN6937569.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGRGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            7365..7583
                     /locus_tag="ACHGNS_001624"
     CDS             7365..7583
                     /locus_tag="ACHGNS_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFN6937570.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            7603..8493
                     /locus_tag="ACHGNS_001625"
     CDS             7603..8493
                     /locus_tag="ACHGNS_001625"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFN6937571.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            8511..8795
                     /locus_tag="ACHGNS_001626"
     CDS             8511..8795
                     /locus_tag="ACHGNS_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937572.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            9282..9896
                     /gene="gmk"
                     /locus_tag="ACHGNS_001627"
     CDS             9282..9896
                     /gene="gmk"
                     /locus_tag="ACHGNS_001627"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFN6937573.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            9901..10206
                     /gene="rpoZ"
                     /locus_tag="ACHGNS_001628"
     CDS             9901..10206
                     /gene="rpoZ"
                     /locus_tag="ACHGNS_001628"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFN6937574.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            10459..12867
                     /gene="priA"
                     /locus_tag="ACHGNS_001629"
     CDS             10459..12867
                     /gene="priA"
                     /locus_tag="ACHGNS_001629"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFN6937575.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            12882..13373
                     /gene="def"
                     /locus_tag="ACHGNS_001630"
     CDS             12882..13373
                     /gene="def"
                     /locus_tag="ACHGNS_001630"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFN6937576.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            13366..14304
                     /gene="fmt"
                     /locus_tag="ACHGNS_001631"
     CDS             13366..14304
                     /gene="fmt"
                     /locus_tag="ACHGNS_001631"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFN6937577.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            14304..15662
                     /gene="rsmB"
                     /locus_tag="ACHGNS_001632"
     CDS             14304..15662
                     /gene="rsmB"
                     /locus_tag="ACHGNS_001632"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFN6937578.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            15675..16415
                     /locus_tag="ACHGNS_001633"
     CDS             15675..16415
                     /locus_tag="ACHGNS_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFN6937579.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            16412..18481
                     /gene="pknB"
                     /locus_tag="ACHGNS_001634"
     CDS             16412..18481
                     /gene="pknB"
                     /locus_tag="ACHGNS_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFN6937580.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            18646..19545
                     /gene="rsgA"
                     /locus_tag="ACHGNS_001635"
     CDS             18646..19545
                     /gene="rsgA"
                     /locus_tag="ACHGNS_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFN6937581.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            19558..20208
                     /gene="rpe"
                     /locus_tag="ACHGNS_001636"
     CDS             19558..20208
                     /gene="rpe"
                     /locus_tag="ACHGNS_001636"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFN6937582.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            20209..20847
                     /locus_tag="ACHGNS_001637"
     CDS             20209..20847
                     /locus_tag="ACHGNS_001637"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFN6937583.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            21002..21856
                     /gene="mreC"
                     /locus_tag="ACHGNS_001638"
     CDS             21002..21856
                     /gene="mreC"
                     /locus_tag="ACHGNS_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFN6937584.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            21860..22369
                     /gene="mreD"
                     /locus_tag="ACHGNS_001639"
     CDS             21860..22369
                     /gene="mreD"
                     /locus_tag="ACHGNS_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFN6937585.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            22624..24198
                     /locus_tag="ACHGNS_001640"
     CDS             22624..24198
                     /locus_tag="ACHGNS_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFN6937586.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT         8137 a         4395 c         5203 g         6469 t
ORIGIN      
        1 aatgctcgaa tttttgaaga aaaacttata taatgtaaaa cgataaaatt tcttaaaagg
       61 agttggaata ggtgaaagta cgtcgcagtg aacgtctagt tgacatgaca cagtatttat
      121 tgaatcaccc gcatgaattg atttccttaa caagctttgc cgaacgttat gaatctgcta
      181 aatcttctat cagtgaagat ttgggaatca tcaagaaaac atttaaagaa cgagggtatg
      241 gaaccttgga aacaatcccg ggtgctgcag gtggtgtatt gttcatccct gaaatttcat
      301 atgaagaggc gaaaaaatat attgaatctc tagcagaacg tctatcagaa caagaccgtc
      361 tgcttccagg aggatatgtt tatctctctg atttgttagg agaacctgaa ctgctgcgtc
      421 aagttggaag aatcattgct tctaaatacc ttggtgaaaa aatcgatgct gtaatgactg
      481 tagccacaaa aggcgtacct attgcccaag ctgtgtccta ttatctgaat gcgccattcg
      541 ttattgtacg ccgggactca aaaataacag aaggatcaac agtaagtgtc aactatgtgt
      601 ctggatcttc tgaaagaatt gaaaagatgg aactgtctaa gagaagtttg aaacgtggtt
      661 cacgtgtcct tgtagtggat gatttcatga aaggcggcgg aacagtcaac ggaatgaaga
      721 gcttgattga agaattcgaa tcagaacttg ttggcatcac tgtatttgcc gaatcaaaat
      781 tcaacggtca tcgcgccatc gatgattata cgtcactgct ttatgtcaaa gatgtagata
      841 ctcaaacgaa aaatattacg gttgtgccag gaaattattt ttcagaataa gaggttgtga
      901 aaaagccgct tagtttcgag tagtaagaaa aattttgtga aaatggcttt ccacattttt
      961 tcataaattt ggcttaatac cgagaaactg gcttttgaac accgtttatc tagaggttgt
     1021 gaaatcagcg tcctgtcctg agtattagct caataaacgg aaaaattgct cctcatattt
     1081 tgccgtttat tgagctaata ccgaaggtca gctgattgaa aaacgtttaa tgaagaggtt
     1141 gtaagcctac cgtttaactc taagtcacaa gaaacgattt caaaaaacag cttttcatgt
     1201 tttatgagta gtgtgacttg tgaccgagga gttggcagga gagcaccggt taccaaatag
     1261 ataggcccgt tatggtctat ctattttttt atccagaaaa cgctgggata aaagaaaatc
     1321 acttgttttg ttgaaaaaat gggtatactg ggataaactg aaatcagcaa actatattta
     1381 ggagtgacgc agttggacgc acgttatgca attattttgg cggcagggaa aggtacccgc
     1441 atgaagtcaa aattatataa agttttacat cccgtttctg gtaaacctat ggtagaacat
     1501 atcattaatc gggtcagtga aacgaaacca gatgaagtga ttacgatcgt tggacacgga
     1561 gcagaacaag ttaaagcaca actaggagaa cgcagtaaat atgcgttaca agcagaacaa
     1621 ttgggaacag gacatgctgt tttgcaagct gcttctttct tggaaggaaa aaaaggaaca
     1681 acgttagtta tcagtggcga tacaccatta ttgactactg aaacgctgaa caatcttttt
     1741 gaatatcacc aaggaaaaaa tgcaagtgca acgatcttga cggcacaagc agaaaaccca
     1801 acaggatatg gacgaatcat ccgtgaccat attgggatcg tagaaaaaat cgtggaacaa
     1861 aaagatgcaa caccagaaga agctcttgta caagaaatca atacaggaac ttactgtttt
     1921 gacaacgaag ctttgttcga tgcgttaagc aaagttggaa caaacaatgc tcaaggtgaa
     1981 tactatctaa cagatatcat cgaaatctta aaagaagaag gccatacagt tgctgcttat
     2041 caaacggatg attttgaaga atcaatggga gtcaatgatc ggatcgcgtt agctaaagca
     2101 aatgagatca tgagaaagcg aattaaccag atgcatatgg tcaatggcgt aagctttgtc
     2161 gattcagcaa cgacatacat cgatgcaggc gtagaaatcg gtccagacac attgatcgaa
     2221 gcaggtgtac aaatccaagg aaatacagtc attggttctg attgtgtcat cgggtcacat
     2281 tcaaaaatcg tagacagtag gattgaggat cacgtcgtca ttgaaaactc agtcattgaa
     2341 agcagccatg tcaagaaaca tgcagatgtg ggaccttatg cacatttacg accaaaagct
     2401 gaaattggcg aaaacgtcca tatcggaaac tttgtggaag tgaaaaatgc tcaaatcggc
     2461 aaaggaacta aagtcggtca cttgacttat gttggtgatg ctacgctggg agaagaaatc
     2521 aatgtcggat gtggtgttgt cttcgtcaac tatgatggaa agaacaaaca tcatacgact
     2581 gtcggcgacc acagctttat tggttctagc acgaatatca tcggacctgt cgaggtagct
     2641 aaaaacagtt cgattgctgc cgggtcaaca attacagaca atattccaga atatgcgctg
     2701 gcaattgcga gagcaagaca agtcaataaa gaaggatatg caaaaaaact cccttatcta
     2761 aactaagact tttgcaaaat cacttgattt atttaaaaaa tatgactact atttattagg
     2821 tagaattcta gagttgtgaa aaagaaagtg gaggtcccca tgtcaaaaca ttactttgac
     2881 ccaagattaa aaatttttgc gttaaattcc aatcgcccat tagcagagaa aattgcagca
     2941 gcagtcggcg tcgagttagg aaaatcatct gttactcaat ttagtgatgg agaaattcaa
     3001 gtaaacattg aagaaagtat tcgtggatcg catgtctatg ttattcaatc aacgagcagc
     3061 ccagtcaatg ataacttgat ggagttgctg atcatgattg acgcactgaa acgagcaagt
     3121 gcaaaaacaa tcaacgtagt catgccttat tatggctatg cgcgtcaaga ccgtaaagca
     3181 cgtgcacggg aaccaatcac agcgaaatta gtagcaaaca tgattcaaaa agctggtgca
     3241 acaagaatgc tgactttgga ccttcatgca gtccaaatcc aaggtttctt tgatattcca
     3301 gtcgatcatt tgatgggtgc gccacttatt gccaattatt tcttagaaaa aggaatcaaa
     3361 ggcgatgatg ttgtcgttgt ttcaccagac catggtggtg tcacacgggc acgtaaatta
     3421 gcggaatttt tgaaatctcc gattgcgatc atagacaaac gccgtccaaa agcaaatgtt
     3481 gcagaagtaa tgaatatcat tggacaagtc aaaggcaaaa cttgtgtact gatcgatgat
     3541 atgatcgata cagcaggtac gatcacattg gctgccaatg ctttgaaaga agctggtgca
     3601 actagtgtgt atgcttcatg tacacatcca gtattatccg gccctgcttt acaaagaata
     3661 gaagattcag ctatcgagcg tctagttgtt acagactcga tctatcttcc agaagatcgt
     3721 aaaatcgaca aaatcgatga agtaagtgtt ggcggactaa tgggagatgc aatcaaacgt
     3781 atccatgaaa acaaaccagt tagtccattg tttgaaacaa aaaataaata ataaataaac
     3841 tcacaaaggg gagtccaatt gggctgagat tgaatctatt tctaaaccct tcgtacctgt
     3901 atcggttatg cgagcgtagg aattgtgaat aaacagctgt ttgactattt tcgaaagctg
     3961 cttcctcctt tgtgactttt ttattcaaag gaggaagttt ttttatggga aaaaatagag
     4021 tttgggtaga aggaacgatt gttgcagcgt tagcgatggt gttgtcgttg attccgatcc
     4081 agatcggcag cagtttttct atttcattag ggcagattcc attgactctt tttgcgttaa
     4141 gaagagggac aaaggcaggg atgctggcag gtttcatttg gggcatccta cattttccgt
     4201 tgggacaagt ttattattta agtgtcgttc aagtgttgat tgagtacccg attgcctata
     4261 cttttgcagg cgcagcaggg ctcatggctt caaatgtcca aaaaggctat gtagacgata
     4321 atacgaaaca aattagaaaa agcatcgtgt ttggtgcatt gattggcgca ctttccagat
     4381 atttttggca ttttattgca ggagtggttt tctggggagc gtatgcacta tgggggatga
     4441 atccttggct cttttctttc gtaatgaatg gtgccagcgg gttgagtaca gctattgtaa
     4501 cgatcatcgt tttattaggg atccaacaaa cgactccttc gatttttgtg ccagataaac
     4561 gacaggagct gtaaataaaa aagaagcaag aaacttgaga cagaatcatg tcctagtttc
     4621 ttgtttcctt tttatttttt gataagaaat agatctactt caatggcaga taagaaagat
     4681 cggcatcttc taccaattgc cagtgtcgat cggtgacaga gtagattaaa gtagcgatga
     4741 tttcctcttc tcgataccgg gtgcctttta atttccaaat ataatcttca atcacataga
     4801 ttgggatacg atatttacca tcgatcaaac caacttgatc aaaagaaaaa atgatccctt
     4861 cctgcatcaa attacgcatt tctttcaata attccacact gattttttca acaggtgttt
     4921 cttttttgaa aaaagatttt tttacattga tcttatctga caagatcgaa cttaatttac
     4981 tagcaaaaat cctataaaat tcatctaaat agcgataata aatccgattc agctgatcat
     5041 taagatcgaa gtatacaaaa ttattctgta gtttataaaa aaacggcgaa tgaagatgtg
     5101 ttttcatatg accaaaatat aataattctg aaatttctaa aggtgtcaat tctttcagca
     5161 tcgttaaatc agtaaagtcg atccacttca attcatcgaa tccacgcctg ttacgagaag
     5221 aggaaaagaa acgctgaata ttttctgtac cacggattac tttcagccct gtatgggatt
     5281 catattctcc agcagaagca gatggatcaa gcaagagcaa gttttgagga aaatgcacga
     5341 tactgtttgc aaagtcagta gcagtcagcc cttttgttaa aacagcatta cttgtggtat
     5401 caatatgtac ataaactgac tcagtcatca tgttcctcct cttatttcct cttaatctct
     5461 tttattttac aacatttgca ttaaaaaagc ttgtttcttt tattttaaaa ttccattaca
     5521 aaatcacgtt ttcattgaaa acactctcat tttcaattaa tttttgtgat ttttgtattt
     5581 ttttatttaa aggaggtttt atgaattgtt ttcttatgga atctacaaag tcgaatacga
     5641 gactcatgac ctttttatga cgtactggta tacttgaaga aaaaggagag cgcgatggag
     5701 ataaaaaaag tttacttttc acagaaaatg tcgctgtatt cgcctatcca gcaagcaaaa
     5761 caaccccttc cattttttca aagtcatcaa gaaagtagag acgctttttg gcgttctgca
     5821 aaaaagcaat gtgcttttaa agcagtatcg aaaaaatata ccgtctattt tactattgct
     5881 aaaagtagag gagatgttat ttttaataat ctgttgattg aaggtgaaac ttttgaatgg
     5941 aaaaaatttt ttcggtatat ggaagcttgc gctcgttttt ttattaaaga aaactgttgt
     6001 tttttatttc aatctcctct atcagaagaa tggttgcgta tttttcataa aaacggctat
     6061 caaggaacca cacaattaaa taaaaagctg gtctatcata cagcacttgt tttaggtaga
     6121 ggaggagcgc acggtgctta tcagattggt gtgtggcagg cgctgaagga acatgatatc
     6181 aactttgaga ttatcacagg tacctcggta ggtgcgttga acggcgcatt gattttacaa
     6241 ggagatatga aaaaagctgt gaatttatgg aaaaagctgt cgacaagaca agtgcttgcg
     6301 ttaccagaaa tggctgcagt tgaagacctt cgtgaacgct tctatcgaga aagacgccaa
     6361 atgacgaaaa cagcactaat tgaaggcggg gtttcttctg ctccattgga aaagatggtc
     6421 aaagacaatt tggatttgtc cttactcaaa cataatccca aaatacgttt atatacggtg
     6481 tctactaaac tgccagaact agcggaagta gtgactgata tccaacagac aaaaacagaa
     6541 gaaataccag attggatact cgcctctgct tcattttatc ccgctatggc ttatcgaaag
     6601 atcggaaaaa ataaatatgc agacggtggg tatcgcaaca aaatacctat tgatattgcg
     6661 attaacaaag gagcaacaga ggcatttgtc gtagatgtgc aagggccagg cccagcgaaa
     6721 aaaattcgta tgccagacat ttttatccat tggaaatgtc aaacattgtg gacactaggc
     6781 agctttctgc tttttgatag ccaaaggaac caattaaatt tacaattagg ctatctggaa
     6841 atgaaaaaac gattaggcta ttactatggg aactggtata catttcattc tgttaaacct
     6901 gcaagaacat actggcgagg atttttgtct tacttaacaa aagaaattca gttagaatta
     6961 tcttttttca aaagcataaa attttggcaa aaactgcgta atttatataa gaacagggtc
     7021 gtaccagaga catgcggatt ggcgatgcta gaacttttag caaaaaaaca ttttttactg
     7081 cccaatgaaa tctatcaagt agacatgatg attcaaatga tctgccaaca agatataaaa
     7141 agtatgccgg atgatttatt ggcacaaatt ggtcaattaa gtactgaaga atggcgaagg
     7201 tatcgaaaat accagcaaaa aatacaaaat gagcgcacga aaactgttta ttttgattat
     7261 ctgctacaag gaaaacgcaa ggatcgtttg cagcatgaat tgcttaagca accagttgac
     7321 acattactga tcctatattt atattatctc aaggaggaac aaccatggca caagaatttt
     7381 catatgaaat cgtagaagaa atcgctatat tgtcagaaaa caacaagggg tggcgtaaag
     7441 aactgaacct agtcagctgg aatggtcgcc cgccaaaatt cgatcttcga gattgggcgc
     7501 cagaccatga aaaaatgggg aaaggcctga cattgacgaa cgaagagttt gaacaactgc
     7561 aaaaagcaat agaaaatatg taatgaggaa ggaccctgtc gaatgaaaag tatgactggt
     7621 tttggcaaag ccatccgcga aacaaaagcg tatcagttag atattgaaat aaaaagtgtc
     7681 aaccagcgtt ttctagatgt tcaaatacga agtcctaaaa tcctgaactt tgttgaaaac
     7741 gattttcgtc agcttattaa acagcagtta agccgcggtc gaatcgaagt atttgtgaat
     7801 ctagcttata ttggcaaaaa cgaaaaacag atcgtcatca actgggaatt gattgattca
     7861 cttatcactc aattgacaga cggaattcaa caacgatacg gtagtcaaaa tcaagtgtca
     7921 attagtcgga tggcggaacg cttcgtgctg gacgaaaact tcgtaacggt agaagaaaaa
     7981 ccggaagaga gcatggaaga attggcacag ttagctctag atacattgaa aagcgcactt
     8041 gattctattg aggcgagtcg agaaaaagaa ggtcaagctt tagctttagt gttgaaaaag
     8101 aatactgaag aattcaaaga agtactaaaa aaactcgata gttttgtaga aatctatgaa
     8161 aaggaatttc aaataaggta tcaaaaaaag ctggaggaat ttcttggagc gaaagtcgat
     8221 gagcaacggt tgttgacgga gctagccatc ttgttggaac gcggagatat tcacgaggaa
     8281 ctggatcgtc tggcgatcca tgtacaaaaa ctagatgaac ttatcgaagc acaatgtcct
     8341 gtaggtcggg aattagattt cctgatccaa gaaatgaatc gggaaatcaa cacgatcggt
     8401 tccaaatcaa atgcaatcga aattaaaaat caagtcgtcc agtctaagac gatcttagaa
     8461 aaaatcagag aacaagtaca aaatattgaa tgacgaaaga ggtagagata atgaaatatt
     8521 atgacgtaac gttccacgaa ttaagtggaa aaactgtggt taaacgggat atcccttctg
     8581 aaaaaaacgg ctttgacgta tggaaagatg cctgtgcaga ctataatgag aacgaactgt
     8641 ttattttgat caacgacggt gcttacgtga cgatgaatcg gaaatttatt gttcgaattg
     8701 acacagaaga ggtagaagat ccgacagaaa aagcacgttc tcgaaaagat gagatcatgg
     8761 gtgtagtgaa tactttgtcc aatatgggct tttagcaagg atgctgaaca aagaaataca
     8821 ataaataaac agaattggat ttcttcgctt agcttctttg ctctaggaat acatttaaag
     8881 gttgtgacaa aattcgtgtc acaatctttt tttattttga ataagtgatg ttcaaaaacc
     8941 attttctcgg tattaagcca aatttatgca aaaatgtgag aaactatttt cacaaaattt
     9001 ttcttcatac tcgaaactaa tcggcttctt cacgacctct tatacacggc gttcaaaagc
     9061 ttgcatctgc gataataacc ccaaccaaac gaaaaacatg agaagctgtt tatgtttggc
     9121 tgttcttatt acttgatgca ggacagcttt tttacgacct cttggtacag ggtagctttt
     9181 ttaccaattt ctagctataa ttaaagataa ccttgaaagt tgcagaaaaa aagtgcaaaa
     9241 taaacgataa gtacaaaaag actagttgaa aggacgtcgt catgtcagag cgtggattat
     9301 taatcgtgtt gtctggtcca tccggtgtag gaaaaggcac agtacgaaaa gcaatttttg
     9361 agagtgagga taatgatttt cagtattcga tctcaatgac tacccgtaaa atgcgtgaag
     9421 gggaggtcga aggcgttgat tatttcttcc gaagcaaaga agagtttgaa tcaatgatcg
     9481 aagctggaga gatgctggaa tacgcagaat atgtcgggaa ttattatgga actcctttgt
     9541 catatgtcaa caagacattg gatgaaggaa aagatgtttt cttggagatt gaagtccaag
     9601 gagcaaaaca agtcaaagaa aaagtgcccg atggtgtatt catctttttg actccgccag
     9661 atttagcgga attaaaatca cggatcgtag gccgaggaac agatgcagat gaagtaatcg
     9721 aaaaacgaat gaaagttgca aaagaagaaa tcgaaatgat ggctctttac gactatgctg
     9781 ttgtaaatga tgaagtgccg ctagcagttg aacgaatcaa aaacattatt gccagtgaac
     9841 attttcgagt agatcgcgta atcggtaaat atattaaaat gttaaaggag atgtagtctt
     9901 atgatgttaa aaccttcaat tgactcacta ttagatcgtg taaattcaaa atattctcta
     9961 gtgattttag caagtaaacg cgcgcatgaa ttggatgcaa gtgcacagcc tactctagat
    10021 tcttttgatt cagtaaaaag cgtcggtcaa gctttagaag aaatcgatgc aggaaatgta
    10081 gtcaatgatc cacatcctga attaaaacgt gaacgtttga agatggaaga agaggaacgt
    10141 caagcacaaa aagagcgtga acaacacgaa ttggaatcac gtatccgtga agagcaaaaa
    10201 atgtaataaa gaaaaataga gcgaacatct aaaaagaaaa gcaaacccag ctgtttcctt
    10261 ttcaaacatt cgccacttat tgtctggtag aaaaggctgt gtctgaactt tttgacacag
    10321 ttcttttttt tcttatctgc tgttaatttt ttgttaggat ttggcagaag ataagacaaa
    10381 tccatcgatc tctagtaaaa taggcaagga actttttgtc gcttgataag aaaagacaaa
    10441 aaaaggagga aacgaaccat gacattaaca gctgaagtca ttgtggacgt acctactatg
    10501 cagaccgatc agccattcac ttatttggtt ccctctgaaa tggaaacagc tttgcaagtc
    10561 gggatgcgag tagaagtccc ttttggtaat ggcaatcgtc atgtccaagg atttgttatg
    10621 gctatccaaa aatcagaaga atcagctaat ccatctttga aggcagttat tcgtttgcta
    10681 gaccttgctc cggtggtcaa tgaagaatta ctttcattag ctgattatat gaaaaaaatt
    10741 acttacgcat tcaaaatcac ttgtctacaa acgatgcttc cgagtgtgat gaaagcagaa
    10801 tatgataaat tgatctatcc tttggcagac acaccagaag ttcaggtatt gtttgatcag
    10861 cgagaagtga tttcctggaa ggaagcagaa gaagaaggca gcctttctca attgattcgc
    10921 tggcgccaag agcaattagt agatatcaaa tatgaagtcc atacacgaaa taaagtaaaa
    10981 acgattcgtt tggtccgttc tttgttaact gaaaagcaaa tcgaagaaga atgggcaaaa
    11041 ttacggcaaa atgccaagaa acaaaaagaa cttcttttgt gtttatctga actgagccaa
    11101 gaagaaccaa ttgcttattt caaaaataaa ggaatcagta ctgctgtctt gaatcaagga
    11161 aaagaaaagg gctggctgga gtttatagaa tcagaacgtt atcgcgaccc ttataaagac
    11221 cgagtatttg accaaacgac ggcattggaa ctgaatgcag aacaaaaaaa tgctgtcgaa
    11281 caaatcatca cagcaggtca gcagcaaaaa gatcaggtct ttttgctgga gggaatcact
    11341 ggcagcggga aaacagaagt atatcttcag gcaatcgctg atgttttgtc agaaaataaa
    11401 acggctatta tgttagtgcc tgaaatcgca ttgactccgc aaatggtcga gcgattcaaa
    11461 ggacgcttcg gagaatcagt tgcggtctta catagcggac tttcccaagg tgaaaaatat
    11521 gatgaatggc gcaagattga gcgggaagaa gcgcaagtag tagtaggcgc acgatctgct
    11581 atttttgccc cgctgaaaaa tatcgggctg atcattatcg atgaagaaca tgaatcaagt
    11641 tacaaacaag atgagacacc gcgttatcat gcgagagatc tagcaatttg gcgcagtaaa
    11701 tatcatcatt gcccaatagt tctaggaagc gcgacgccct ctttagaatc aagagcacga
    11761 gctcaaaaag gcgtttatca gttattgcag ttaaaccacc gagccaaagc agctgcacag
    11821 cttccagcta ttgagatcgt agatatgcgt gaagagtttc agaatcatcg gacctctact
    11881 ttttctgcta atttgcagga aaaaatacaa aatcgattag acaaaaaaga acaaactgtc
    11941 ctactgctca atcgtcgagg atattcgtct tttgtgatgt gtagggactg tggatttgtt
    12001 cttccatgtc ctaattgcga tatctctctt acactgcata tggatacacg ttcaatgcgc
    12061 tgccattatt gcggacatga agaacctatt cccaatcgct gtcctaattg tggcggcaat
    12121 aaaatccgct actatggaac aggtactcaa aaagtagaag aggaactccg agaactttat
    12181 ccgcaagcta ggattttgcg tatggatgta gataccactc gaagaaaagg ggcacacgaa
    12241 cagattttac aaaaattcgg tgcaaaagaa gcagatatat tattagggac acaaatgatt
    12301 gctaaaggtt tggactttcc agaagtgaca ttagttgggg tgttgaatgc tgatacctcc
    12361 ctgaatttac cggattttcg ttctagtgag catacattcc aattgcttac ccaagtttca
    12421 ggacgagcag ggcgagcgga aaaagcaggc gaagtcgtga tccaaacatt caatcctcaa
    12481 cattacgcca tagaattggc taaaaaacaa aattatgagc agttttatca gcaggaaatg
    12541 catgtacggc atcgtggagg ctatcctcct tattacttta ctgtgaaaat aaccgccagc
    12601 catcctgaag aacaagtagc ggcaaagaaa atctttcaga ttgccaatca gctaaaagaa
    12661 gtgttaagcc ctcaaagctt gctattaggg ccaacgccca gcatgatttt gagagtgaaa
    12721 aaccgatatt attatcaact aatcatcaaa tataaacatg aaccgaatct tcctcatgtt
    12781 ttggatgaaa ttttgaatgg aagccagaaa gaacagcgac aaggtctttt tgtggcaatt
    12841 gacaacgaac cattaaactt tatttaagaa agggccaaat tatgcgctat cctatattga
    12901 tccaccctaa tgataaatta aaaagaactg ctcagccaat cgatgtaatc acggacgaga
    12961 caatcgcttt attagacaat ttatatgaga caatgattgc gaatgacgga atagggatcg
    13021 ctgctcctca agtaggacaa aacaagagaa ttgctgtgat cgaagtagat gaaggcgaga
    13081 aatttgaact gattaaccca gaaatcatcg aagcaaaagg tgaaagcctt gacgtagaag
    13141 gttgtctcag tattccgcat gtctatggaa cggtcaagcg agcagatgaa gtgaccgtgc
    13201 gttattatga tcgagacgga gaagagatag aagtgacggc ttttggctat ttagcccgtg
    13261 ccttccagca tgaaatcgat cacttagacg gtatcttgtt cattgagaaa atgatacagc
    13321 agatcccgga agaagaactt gaagaatata tggaggaaca tcttgatgac taagctagta
    13381 tttatgggca cacctgcttt ttctgtgccg attcttgaag ggctgataga atcaggttac
    13441 gagatccagg cagtggtaac acagcccgac cgtccagttg ggcggaaacg agtaattacg
    13501 ccgacaccgg taaaaaaagc ggctctgaag cacggtatcc gtgtgctgca gccagagaaa
    13561 atctccggtt cgccagaaat ggaagaaatc attgaattgg tgcctgatgt gatcgtcact
    13621 gcggcatttg gccaattctt gcctgaaaag ttattgcaag taccaaaatt aggggcaatc
    13681 aatgtccatg cttctttact tcctaaatat cggggaggtg cgcctgttca ttatgccatc
    13741 atgaatgggg agaaagaaac tggtgtaacg atcatggaaa tgatcaaaaa aatggatgca
    13801 ggcggaatct atgcgcaaga aagcatacca atcactaaac aagatgatgt agggacgatg
    13861 tttgaaaagc ttagcctttt aggaagaaag ctattgctag aaacattgcc caatattttg
    13921 gatggacaaa aaccagtacc tcaagacgag tcagaagcaa ctttttcgcc aaacatcaca
    13981 cgggaacaag aagcaatcga ttggcataaa actgcagaag aaattgacaa ccaagtacgc
    14041 ggaatgcgtc cttggccaat tgcattcacg acctatgagg gcatacgctg gaaattatta
    14101 tcggttgaag tattggcaga aactacagac aaagcacctg gtacgatcat ccggaaagac
    14161 aaaaaaaatc tatggattgc ttgcggtaaa cagaccgtct tagccatcaa tgaattacag
    14221 ccagctggta aaggaaaaca ggccgtacat gatttcctta acggaagcgg tcagcaagtt
    14281 cagattggtc agcaggtgaa ataatgaaaa aaatccctaa aaaaatgaaa aaatcagtac
    14341 gatatatggc attactgaca ttagagcgag tgaacaaggg tggcgcctac tcgaatcttt
    14401 tgttgaatga gatgatcaaa caaagcgagc taaatcaaaa agatgtgggt ttatttacag
    14461 aattgatcta tggtacgatt agccatcagc gactattgga attttacgtt gcaccattga
    14521 tcgctaaagc aaaaaaagtg gatgactggg tcaagatgct tctttatctt tctgtatacc
    14581 aattagaatt tttagataaa gtccctgcac atgctatttt aaacgaggcg gtcgaaatcg
    14641 caaaagtaaa aggaaatcct gggacaggga aatttgtaaa tggtgtcttg aggaattatc
    14701 agcgtcaagg agcaccagat ctgcaaggca ttgctgatcc tatcgaaaga ctttctgtca
    14761 aaatcagcct gcctctatgg atgacgaaac gactggtgga tcaaatcgga tacgaagaaa
    14821 ctgaaaagct cggtttatcg ctatatcagc ctagccatgc tagtgcccga gtagatacac
    14881 gccgactttc cagagatcag gccatcactg ttttgcaaga agaagagatc acagctgaaa
    14941 aaagccaagt cagtccatac ggtatagtag cgaaaaaagg gcatttagct tctagctcat
    15001 tgttccacga tggcgtcatg acgatccaag atgaaagttc gatgcttgta gcgccgtcta
    15061 tgcaaatcga gcctgaacat catgtcttag atgcctgtgc agcacccgga ggtaagacga
    15121 cgcatattgc ttctttttta gatccgtcat gcggtggaga agtggtcgct ttagatgtac
    15181 acgaacacaa agtcaaactc attgaagata atgcagcacg tctaggtgtc tcttctgctg
    15241 tctacgctca aaagatggat gctagagaag tcagtaaaca gttcgaagca gagcagtttg
    15301 atcggatttt agtagacgcg ccttgttcag gtcttgggct gcttcggcga aaaccagata
    15361 ttcgttataa aaaacagccg gatgaattag cgaatttacc aaaaatccaa ttggaaattt
    15421 tagaaagctg cgcaccaact ttaaagtctt ccggtattct gacttatagc acttgtacga
    15481 tcttaaaaga agaaaaccaa gacgttgttt ctgctttctt ggaaagacac cctgattttg
    15541 agaggattga tgttttgacc aatcctatgc tacaatcatc aatcgaagag aaaatgttga
    15601 ctttgtatcc gcatcagttt tacacagatg gatttttcat ttgttgtttg cgcaaaaagt
    15661 aatgaggtga aaaaatgcag attgaatatc aatcagatgt aggaagacga cgtaacacaa
    15721 atcaagacta tgccagtgta tttaccagcc gttcaggtct caagcttgct gtattagcag
    15781 acgggatggg tggtcatcgt gccggagaca ttgccagtca gatggcggta acgaatctag
    15841 gcgaagcttg ggaaaaacaa gaagctgttg acgatgaaaa aatcgcccaa tggttcattc
    15901 aatcgattca agaagaaaat atgctgattt atcagcgtgg acaagaacag ccggaatata
    15961 acggtatggg aacaacaatc gtagcggccg ctctgtcaga tgactggttc acgattgcca
    16021 atgttgggga tagcagagct tatttgattc gtgaagataa aatcatgcaa ttgacggagg
    16081 atcattcttt agtcaatgaa ctagtcaaat caggagaaat cagcgaagaa atggcagtga
    16141 accatccaag gaaaaatatt ttaacacgtt ctgtcggcat gcctggaaca gtcgaagtag
    16201 atgtttctac tcatctttgg caactaaaag atcgactgct gttatgttca gatggcttga
    16261 cgaacatgct gtctgaagaa gaaataggaa cgatcatcaa tcaagaaggc ccattgtctc
    16321 ataaagtaac tgagctgatt gatcgtgcca atgaagcagg cggagcggat aatatcaccg
    16381 ttcttcttat cgaatataag gaggacagtg tatgatcgag ttgggcaaaa aattaaatgg
    16441 ccgttatcaa atcactggaa atatcggtag cggtggtatg gcaaatgtat ttttggcgca
    16501 cgatttaatc ttggatcgtg atgtagcagt taaagtctta cgatttgact ttcaaaatga
    16561 ccagactgcg attcgacgat ttcagagaga agctttagca gcaacagaac tcgttcatcc
    16621 aaacattgtt agtgtttacg atgttggtga agaagacaac atgcagtatc tggtgatgga
    16681 atacgtcaaa ggaatggact tgaaacgata tatccaaaca cattatcctg ttccttatga
    16741 aacagcagta aatattatgc agcagatcct ttctgctatt tctttagctc ataaccatca
    16801 aatcatccac cgagatttga aaccccaaaa cgtactgatc gataatgaag gtgttgtaaa
    16861 aatcacggat ttcgggattg ctatcgcttt atctgaaaca tcgatcacgc agacgaatac
    16921 gatgctagga tctgttcatt atctgtcccc tgagcaggcc agaggaagta tggcaacgaa
    16981 gcagtcagat atttatgctt tagggatcat cctttatgag atgttgacgg gtagcgtacc
    17041 gtttgatggt gaatctgctg taacaattgc tttaaaacat tttcaagatg atcttccatc
    17101 tataaaagca cttgatccaa atgtgccaca agcactggaa aacgtgattt tacgggcaac
    17161 ggctaaagaa cctgcagacc gttataagtc agccgaggaa atgtctgatg atctttctac
    17221 agtattatca ccagctcgtg caaacgaaga aaaatggcag cctcatgtga tggataatga
    17281 aacaaaagtt atcacacccc ttacggaaga tgcaccaatg cctgattcat tcaaatctat
    17341 gcctctgcca aaagacaagc cagaaaacga aaccttacca gaggaggaag aagttctgcc
    17401 tgatcctaaa aagaaaggac agaaaaaatg gtggctgatc ctattagcta ttcttgctgt
    17461 tttaggaatc ggaacagctg tattctttgc ttctggcggg cgaggagaag ttgttgttcc
    17521 tgatgtttct gatctttctg aagcttctgc tagagaaaca ctcgcaaaag cgaaattaga
    17581 agtagcaaca aaaacagaag aaattgcaga tgacaaaatc gaagaaggta atgtagtgaa
    17641 gacagacccg gcagcgggaa cgacagtcaa gcaaaaacgc gaagtcactc tgtatattag
    17701 tagcggcacg aaaaaaatca aattagatga ttacagtggt aaaagttatg aagatgccga
    17761 agcaagtttg aaagaattag ggttttctgc tgacttgatt caaaaagaag aggaattcag
    17821 ttcagaggta gaaagcggaa tgatcatttc gcaatctcca tcagaagata cagaagtcga
    17881 tcctaaaaca gacacgatta ctttcacagt cagcaaagga ccagaaacag tgactttgag
    17941 tagtcatgta ggagaaaact acgaaactgt tcgtaattgg cttgttacac aaggattcaa
    18001 tatcaataat attagtgcta cttatgacta tagcgacact gtaccatcag gttcgatcat
    18061 cagtcaggat ccagcctcgg gtgaagttgt agcagaagaa acttacatca attttgtcgt
    18121 aagccaagga acagagccta aattgaatga tatctccggc tatacaaaaa gcgaagcaca
    18181 aagttattta gctagtgtcg gtgcagaata tatcgggcat gagacttccg aatattcaaa
    18241 cacggtggat aaagataaag tgattcgtac agtaccaggg gcaggaacga caattaccaa
    18301 aggaatggtt gtgaacgtaa tttactctaa aggaccagat ccagcgtctt ctagttctga
    18361 aaaagactct tcagacagtt ctagcagctc aagtagctcc agtagcacaa gcaactcttc
    18421 aaaagaagaa tcttccagct ccacaagttc atcagctagc acctcagaaa gttcaaaata
    18481 agtgctgaat aaaatatatc agaagggaat gcgacattga tttgttgcat tctctttttt
    18541 gtgtttctat ctcttttgat caattttcaa gccatatact ttgagaagtt tgcatggaat
    18601 aacacctact ttcctcttcg tatggtaaaa taaacaaaaa cggaggtggc acttatcaaa
    18661 ggacaaatca gaaaagcaat cagcggtttt tactatgtat acgcaaacgg agagacctat
    18721 caaacaagag gaagaggaaa tttccggaat cggaaaatca cgccgcttgt cggagatcag
    18781 gtgatctttg agagtgaaaa tcaaacagac ggctatcttc tggaaatctt gccgagaaaa
    18841 aatcaattag ttcgcccacc agtcacaaat gttgatcttg gaatcattgt cacaagtatc
    18901 gtagagcctg cattttctta taatctactt gaccgctttc tagttacctt ggaatacgag
    18961 tcgatcgaac cgattatttt tttgacaaag accgacttgt cagacgaccc agaaaaaatc
    19021 gaagagatca agcaaaccta tactaaaata ggctatccgg tgattgtacc caatcatacg
    19081 ggtgatacga cagaactgat ccgttacttc ccagaacggc tatcggtctt tatgggtcag
    19141 tctggagcag gaaaatcgac gctactgaat caaatatcac cggaactgaa tttagcaaca
    19201 ggagagattt ctgatgcact tggaagagga cggcatacaa cacgtcatgt ggaactgatt
    19261 cctatctatg gcgggttagt agcagataca ccaggattta gctcgattga ctttttgaca
    19321 attgaagcag gagaactgcc gagacaattt cctgaatttg tagaagcttc tgtccattgc
    19381 aaatttagag agtgcatgca tgctaaagaa cctggctgcg aagtaaaaca tagagtagaa
    19441 acaggtggta tcgcacagac acgttacgac aattatttgc aattcttaca agaaatcgaa
    19501 aaaagaaaac caatgtatgc aaaaaaagaa ggtaaacgaa aataggagga aacaagaatg
    19561 aagatagcac catccatttt gagtgcagat tttgccaatt tacaaagaga cattgagtta
    19621 gtggaaaaag gaggcgcaga ttacatccac gtagatgtaa tggatggccg atttgtccca
    19681 aatatcacat tagggccaaa tattgttcag gcaatccgtc ctgttactaa attaccgtta
    19741 gacgtccatc tgatgatcgt tgatccagaa aactacattc cagcatttgc aaaagctgga
    19801 gcagatatca ttactgtcca cgtcgaagca actcctcata ttcatcgggc acttcaaatg
    19861 acaaaaagct taggagttaa agctggagta gttatcaatc caggaacacc agtctccatg
    19921 atcaaacatg ttttgccttt ggctgatcaa gtactagtca tgactgtcaa tcctgggttc
    19981 ggcggacaaa gctttattga agaaacagta gaaaaaatta cggaactcgc tgatctacgt
    20041 agagagaaca attggactta tgcaatcgaa gtagatggcg gaatcgttcc agaaacagca
    20101 aaaatctgta aagatgcagg ggcggatgtc ttcgttgcag gatcatatat ctatggagcc
    20161 gaaaatccag tcggacaaat cgaacgatta aaagaggcat tagactaaat gaaaatttta
    20221 ttagctgctg gaggtaatcc gcaaaactgg ccgcagtctc tgctgtccaa atacgatgcc
    20281 tatgtcggga tagaccgtgg tgcgctgtac ctgcttcaag caggctaccc agtagatctg
    20341 gcagtgggtg attttgattc attgtcgaac gaagagcaga aggaagtatt tgcaaaagcc
    20401 aaagagtata cccgttcccc tgcagaaaaa gacgatacag atacacagtt aggtatctta
    20461 aaaacatttg aacgttaccc gcaagcggaa gtcactttga ttggagctac gggcggaaga
    20521 ttggatcatc ttttagccaa tctctggtta ggtatggaac ctcgcttcaa accctttgcc
    20581 catcagctta ttatcagaga ttgtcaaaat tatttgtctt attatttgcc tggagagcat
    20641 atagtaagac agataccaga tatgaagtat ttagcttatt gctgcttgac tcccgtctcg
    20701 aatcttacat taaaaggcag caagtatgta ttggaaaata aagaagtaga atatccgaca
    20761 tcttatgcaa gtaacgaatt tctttcagat accgcagaat tttctttttc agatggtatg
    20821 attgcagtta ttcaaagcaa agattgatcc ataaacaaat aaaaaaattt gtctttcagg
    20881 ctaaaaatga cagaatctat tatttgctaa aaaaaagctt aagaggagca acttaccttt
    20941 tgtttcacgg catttagtga taaaattgaa cacaagagtc taattaattc gaggtgtctt
    21001 tgtgaagaag ttcaatccta ataaaaatat tattattacc ttggttattg ttatcatcgt
    21061 ggtgacgata ctaagtgtga ccgctgcgca gcgagcgaat gaaggaaagc ccaatttctt
    21121 ccaatccatc gttaatgaca gtgttgcaat ggttgataaa acaatctctg ccccagtacg
    21181 gtgggtacga aatggagtag attctgtcca taatcttttt tcaacttatg ccgaaaatga
    21241 gcggctaaaa gaaaaaatcg atagctatga tgacgtttca cagcaaaaca agaattacga
    21301 gagagaaatc gaagcgttga aaaaagaact tgagttgaat cagacgctga ctagttacga
    21361 aaaagtaacg gcaaatgtca ttactcgttc gcctgacagt tggcaggaca tgctgatcgt
    21421 agacaagggt tctaaagacg gtatcgaagc aaatatggca gtcatgtcgc aaaaaggatt
    21481 gattggccga gtgatcgagg tcaatacggc ttcgtctaaa atcgaattac tgtcatcctc
    21541 taatgaaagc tccaatcatt ttccagtacg ggtatcttcg gctaatggcg aagcgtttgg
    21601 tttgcttaaa aactatgatg aaaagctcca tgccttagtg gtgacccaat taactggtga
    21661 tacggatatc aaagaagggg atgttgtcca gacatccggt cttggaggga attctccagc
    21721 taacttgccg atcggtacgg ttattaaaac gaaaccagat agttatgggc tggatcggga
    21781 agtttatgtg aaaccttatg cagaaatgta tgacgtgtca gttgtgacga ttgtccaacg
    21841 gctagcggag gattagtcca tgttgaagaa agaaacaatg aagtactatc tgccaatcgt
    21901 tttgttcttt ttgatgttga tagatggtca tttaacaaga atgctagggg agtggtcgaa
    21961 aggcacctat atgtcaaatg cccactttct gatattggca ttattatgtt gcagtatggc
    22021 gtttgaaaaa cgttatttac tgattaccac gattgttctc ggggctatct atgacgctta
    22081 ctatattggc gttatcggta tctatgcagt agctctccct ttaattgtat ggttgatgta
    22141 tgtaatgaaa gacgttatcc atgtcaacat ctttactgaa tttttcagta tgatcatctt
    22201 tgtcacgggt tatgaattgt ttacgatggt ggtccagttg atttttaaat tagcagtagt
    22261 aaataacacg tattttatta caaggttttt aggacctaca ctgctgttga acatgattat
    22321 atttgtatta ttcatttttc cctttaagaa attattcagt gatgaataat ttcttttttt
    22381 atttttaggt cgaaagcgaa aaagaatcgt tgttatctta agaaatgcta gacttaacgc
    22441 ggctttttca tgatcatttt ctgaaacgaa cagttttgtc atttttaaaa gttgttttga
    22501 aacatttttg taacataact attatatcgt tgacacactg ctatggtaca atttttatat
    22561 cgttaagaaa gacttaaata tatttagata tttattaaat taagccagtc ggaggaatga
    22621 agagtgaaaa agagtttaat atcagcagta atggtatgtt caatgacctt gactgccgta
    22681 gcatcaccga tcgctgccgc ggcggacgat tttgattctc agatacaaca gcaagatcaa
    22741 aagattgcag acttgaaaaa tcaacaagca gatgcacaat cacaaatcga tgccttagaa
    22801 agccaagttt ctgaaatcaa cacacaagca caagacctat tagcaaaaca agatacattg
    22861 cgtcaagaat ctgcacaatt agtaaaagac attgcagatt tacaagaacg tattgaaaaa
    22921 cgtgaagata caatccaaaa acaagcacgc gaagcacaag taagcaacac aagctcaaac
    22981 tacatcgatg ctgtattgaa tgctgattca ttagcagatg ctattggacg cgttcaagca
    23041 atgacaacga tggtaaaagc aaacaacgac ttgatggaac aacaaaaaca agataaaaaa
    23101 gctgtagaag acaaaaaagc tgaaaacgat gctaaactaa aagaattagc tgaaaaccaa
    23161 gctgctttgg aatctcaaaa aggcgatctt ttatctaaac aagctgattt gaacgtactg
    23221 aaaacttcat tagctgctga acaagcaact gctgaagaca agaaagctga cttgaaccgt
    23281 caaaaagcag aagctgaagc agagcaagct cgtattcgtg aacaacaacg tctagcagaa
    23341 caagctcgtc aacaagctgc acaagaaaaa gctgaaaaag aagcacgcga acaagcagag
    23401 gctgaagcac aagctactca agcttcatct actgcacaaa gctcagcaac agaagaatca
    23461 tcagctacac aatcttcaat gactgaagaa tcatcatcag ctacacaatc ttcagcaaca
    23521 gaagaatcaa caacacctga atcttcaaca gaagaatcaa cagcgcctga atcttcagca
    23581 acagaagaat caacaacagc gcctgaatct tcagcaacag aagaatcaac aacagtgcct
    23641 gaatcttcag caacagaaga atcaacaaca gtgcctgaat cttcaacaac agaagaatca
    23701 acaacacctg cgcctacaac accatcaaca gatcaaagtg ttgatacagg aaacggcaca
    23761 ggaagtagta ctccggctcc aacgccaaca ccaacacctg aacaaccaaa acctgtaaca
    23821 cctgctccag ctccttctgg aagtgtgaat ggtgcagcaa tcgtagcaga agcttacaaa
    23881 tatattggta ctccatatgt ttggggcggt aaagatccaa gtggctttga ctgctcaggg
    23941 ttcacacgct atgtttacat gcaagtaact ggtcgtgaca tcggtggttg gacagttcct
    24001 caagaatcag ctggaacaaa aatttcagtt tctcaagcaa aagctggaga cttgttattc
    24061 tgggggtcac aaggcggaac ttaccacgta gcgattgctt taggcggagg acaatatatc
    24121 catgctcctc aaccaggcga aagcgtaaaa gttggttcag ttcaatggtt tgcccctgac
    24181 tttgctgtca gcatgtaaga atga
//
LOCUS       DAZBTF010000039        24180 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000039 DAZBTF010000000
VERSION     DAZBTF010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.039"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            289..606
                     /locus_tag="ACHGNS_001641"
     CDS             289..606
                     /locus_tag="ACHGNS_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFN6937587.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(697..1062)
                     /locus_tag="ACHGNS_001642"
     CDS             complement(697..1062)
                     /locus_tag="ACHGNS_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937588.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1270..1752
                     /locus_tag="ACHGNS_001643"
     CDS             1270..1752
                     /locus_tag="ACHGNS_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937589.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     LVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1949..2839
                     /locus_tag="ACHGNS_001644"
     CDS             1949..2839
                     /locus_tag="ACHGNS_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFN6937590.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            3165..5639
                     /locus_tag="ACHGNS_001645"
     CDS             3165..5639
                     /locus_tag="ACHGNS_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFN6937591.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRRNNTVAMTGDGVNDI
                     LAMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLV
                     KNIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNV
                     MEKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKF
                     RSQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLF
                     ERKNEKEQKPFSRVVKKFLSIFKREN"
     gene            complement(5742..7478)
                     /locus_tag="ACHGNS_001646"
     CDS             complement(5742..7478)
                     /locus_tag="ACHGNS_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN6937592.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNGDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRAYTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLHGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(7475..8179)
                     /locus_tag="ACHGNS_001647"
     CDS             complement(7475..8179)
                     /locus_tag="ACHGNS_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN6937593.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            8310..8813
                     /locus_tag="ACHGNS_001648"
     CDS             8310..8813
                     /locus_tag="ACHGNS_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFN6937594.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            8773..9111
                     /locus_tag="ACHGNS_001649"
     CDS             8773..9111
                     /locus_tag="ACHGNS_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFN6937595.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            9132..10550
                     /gene="ffh"
                     /locus_tag="ACHGNS_001650"
     CDS             9132..10550
                     /gene="ffh"
                     /locus_tag="ACHGNS_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFN6937596.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10662..11240
                     /locus_tag="ACHGNS_001651"
     CDS             10662..11240
                     /locus_tag="ACHGNS_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFN6937597.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            11244..11765
                     /locus_tag="ACHGNS_001652"
     CDS             11244..11765
                     /locus_tag="ACHGNS_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFN6937598.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11844..12398)
                     /locus_tag="ACHGNS_001653"
     CDS             complement(11844..12398)
                     /locus_tag="ACHGNS_001653"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFN6937599.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            12651..12926
                     /gene="rpsP"
                     /locus_tag="ACHGNS_001654"
     CDS             12651..12926
                     /gene="rpsP"
                     /locus_tag="ACHGNS_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFN6937600.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            12938..13189
                     /locus_tag="ACHGNS_001655"
     CDS             12938..13189
                     /locus_tag="ACHGNS_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFN6937601.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            13313..14104
                     /locus_tag="ACHGNS_001656"
     CDS             13313..14104
                     /locus_tag="ACHGNS_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937602.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTATTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            14274..14795
                     /gene="rimM"
                     /locus_tag="ACHGNS_001657"
     CDS             14274..14795
                     /gene="rimM"
                     /locus_tag="ACHGNS_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFN6937603.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14785..15546
                     /gene="trmD"
                     /locus_tag="ACHGNS_001658"
     CDS             14785..15546
                     /gene="trmD"
                     /locus_tag="ACHGNS_001658"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFN6937604.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15682..16029
                     /gene="rplS"
                     /locus_tag="ACHGNS_001659"
     CDS             15682..16029
                     /gene="rplS"
                     /locus_tag="ACHGNS_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFN6937605.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            16339..16581
                     /locus_tag="ACHGNS_001660"
     CDS             16339..16581
                     /locus_tag="ACHGNS_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFN6937606.1"
                     /translation="MNDGKVKQVPSSTKKKNILLKEVLKRFDHGVTYTETEVNSILLN
                     VFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(16669..16815)
                     /locus_tag="ACHGNS_001661"
                     /pseudo
     CDS             complement(16669..16815)
                     /locus_tag="ACHGNS_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            16872..17186
                     /locus_tag="ACHGNS_001662"
                     /pseudo
     CDS             16872..17186
                     /locus_tag="ACHGNS_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345994.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
     gene            17215..18474
                     /locus_tag="ACHGNS_001663"
     CDS             17215..18474
                     /locus_tag="ACHGNS_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN6937607.1"
                     /translation="MKRWKKMMIGSIVLGMSLVFGACGNQEDSSEESANEITVSTWNY
                     ETTPEFKALFEAFEKKTGIKVKAVDIASDDYDTKLTTMLSSGDTTDVLTMKNLLSYSN
                     YALRDQLVDQTERIRELDTDAAEETYEMYDIDGKTYALPYRTDFWVLYYNKKMFDDAG
                     IEYPKNLTWDEYEDLAKKLSKNDGQVYGAYQHIWRSTIQAIAAAQNEANLVEPDYQYM
                     TDYYDRALRMQKEGAQMDFGTAKSTKVTYQSQFEEQKAAMMYMGTWYMAGILANKEAS
                     KTEVEWGITAIPQKKKGESVTTFGSPTAFAVNKNSKKQKAAQEFIEFAASEEGAKVLA
                     GVGVVPSYRTDEIDQLYFNLAGMPTDEISKKAFSPDEIKLEFPIDTYGPAIDKILQEE
                     YDLVLVGDETPEKGTVNMEKRVAAEKE"
     gene            18527..19423
                     /locus_tag="ACHGNS_001664"
     CDS             18527..19423
                     /locus_tag="ACHGNS_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6937608.1"
                     /translation="MENSINKKATNHLRRKRTWIAYSFIAPNFIGFFVFTLIPILFSL
                     VLAFMKWDSFSTPEFAGLDNFRKMANDETFWISLKNTFLYTAAVVPGTLVCSLGLALL
                     LNQKIRGMKLFRTIFFFPYVTSLVAIAVVWNMLFHPTVGPINIFLRRFIEHPPGWTSS
                     SNWALWAIVIVSIWRGMGYYMILYLAGLQSIQKELYEASSLDGANRWKQFLNVTLPSL
                     RPTTFFVTIMLVINCFKVFDLVQVMTDGGPGRATNVLVYQIYNEAFKKFDFGYASAIS
                     LVLFVIVLGVTVVQFSWNKRRT"
     gene            19434..20303
                     /locus_tag="ACHGNS_001665"
     CDS             19434..20303
                     /locus_tag="ACHGNS_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6937609.1"
                     /translation="MEESVRNERKKAKIRSKNMPFIQWLGMILLIVLALLTIIPFLWM
                     VSASFKANNEVLTIPIQWIPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFT
                     SSFAVYGFSELNFKGRDILFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQ
                     AFNAFGVFLLKQYYSSIPDSLCESARIDGLSEWEIYRKIILPLTKPAIASLTIITFVN
                     TWNDYMGPFIYLSSTENKTIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQK
                     YFVEGIASSGMKE"
     gene            20313..20988
                     /locus_tag="ACHGNS_001666"
                     /pseudo
     CDS             20313..20988
                     /locus_tag="ACHGNS_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
     gene            21194..22858
                     /gene="alsS"
                     /locus_tag="ACHGNS_001667"
     CDS             21194..22858
                     /gene="alsS"
                     /locus_tag="ACHGNS_001667"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFN6937610.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            22871..23581
                     /gene="budA"
                     /locus_tag="ACHGNS_001668"
     CDS             22871..23581
                     /gene="budA"
                     /locus_tag="ACHGNS_001668"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFN6937611.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            23711..>24180
                     /locus_tag="ACHGNS_001669"
     CDS             23711..>24180
                     /locus_tag="ACHGNS_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFN6937612.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHADYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFN"
BASE COUNT         7998 a         4135 c         4997 g         7050 t
ORIGIN      
        1 agattacaaa tagtatcgta tttcgcaatc ttaaaattat cttcttactg tgaataagat
       61 aataaaattt gtatttgtcc tttttaggta acttatttta atctatatac ctttctggat
      121 caacgaaagt atactttata tagtcataac gccgatggta actatgcact gtaacgataa
      181 tgctgctaaa atatcttttt acgaaaaaca tcacaaggaa aaacttaact atggtattat
      241 ctaaacataa ttttttcggt atttaatatt taattaggag gtaatatttt gcttttttat
      301 agtaatttta tcttaatagt agcgatatta ttacttctga atatatggat ttttgatagg
      361 agtagaaatg caagtatagg ttttagaact aaacgaagtc tgtcttctaa aaaaaattgg
      421 gtatactcac aaactatttt ttatggagga attgttttaa ttagtttact ttcttcaaca
      481 ctttattctt tgaatattat agatgtttcc acctcaaatt caatatctat tattggaatc
      541 attattgctg caattattac acaactgttt ttagtgtttg gagagaagaa aagaagtaaa
      601 aaatagaaaa actagtgata gttttggtga tataagaaaa acatatatgt ataaaaagaa
      661 gaaagtagct tctctttgta catatatggt ttgtttttat ggatgccgag cttttttata
      721 agataaggct accgcccata acgacccaag gatttgtgga actaagtaaa tacctgcaag
      781 aagcaagatc agctgggtcg gttttaaagg atgatagaag taacgccatt cagtaaaatt
      841 ggcgcaaaat aaataggaag tgacagtaga tataatattg gcaatgaata aagatataat
      901 acgcctgttc ctttgaaaat aatatccagc aaaaagagat aaaaatatca atgtgatgaa
      961 cccgcttacg gaatgataac gaaaatcaag cagtaaggcg aaatgcacat aaggaacaaa
     1021 agataaaagc aacggaaggt ttttccagca agtttttatc atctttcatc accacctaac
     1081 aagtgttatt attttgttag ttttattgta aattgaatcc tgcaataatc aattactttt
     1141 tcattatttc tagattaaat gaataaactc tcatgccatt atctctaaaa aaaaagtttt
     1201 tccccctaat ttttttagat agaaaattgt atagtatgat gagagaaaaa gtagtttggg
     1261 ggaacgttaa tgagaagaaa agcagtaatc gatcggcttt atttattttt ttggtttttt
     1321 cctatcgttt tgctaccttt tacatgggta aatgctgttg tacaaggtgc ggagatatta
     1381 ggcctgatcc aaggaagcgg attagtggtt tctaggaatt ttccagcttt gactgttttc
     1441 tatttagtga taagtgtagg tctcttatta ccaaaatctc tgatcaatca ctacaattgg
     1501 cagctgcttt acttttcttg cttattagta tttacatcta tgttttcttt gctgcctcgc
     1561 ttgattatcg gccctaatgg tttgagcgag agtggattga aggattcagc ttattcctcc
     1621 atgtatttct tgacgatcaa accatggttc tatttagtaa ttgcctcatt tattcttgca
     1681 acattttttt acttctggac agaaatcagg ataaaaagtg aacgatctgt atctataaaa
     1741 aataactatt gatggtggat catcagtaaa aaagcatatg agctgcgtaa aatgcatgtt
     1801 tctcatatgc tttttttatt attatgtaaa gaatgcgaaa aacgtttaca tgatttttac
     1861 ataacttaac tatattttta cagcaaccat acgagggatt tacagtcgac tgataaactt
     1921 tagttgtaaa taagaaagga gcaataaaat gaaaaaagta ttgtttactc taggtatgat
     1981 gggaattctt ttagccggat gcggctcagg atcaggaaat acagatggct ctgccacaca
     2041 aaataattca agtgactcaa atgaacaagt aaaaatcgtt gcggtaggtt cgacagcatt
     2101 acagccgtta gtcgatgcag cacaagaaag ctttgtacaa gaaaatccaa attatcaaat
     2161 ctctgtacaa ggaggaggaa gcggaactgg tttgagccaa gtcgaagctg gagcagttac
     2221 tatcggaaac tctgatgtat ttgcagaaga aagagacgga gttgatgctt caaaattagt
     2281 ggatcataaa gtagcagtgg tcgggatggc accaatcgtc aataaagata cagatgtcaa
     2341 agatatcacc aaacaggaat tgattgatat ctttacagga aaaatcacta actggaagga
     2401 agttggcggg aaagatcaaa aaatcaacgt cgtgaaccgt gcaaacggca gcggtacccg
     2461 cgcgacattc gaaaaatggg gactggatgg tgctacccct gtgcagtccc aagaacaaga
     2521 ttcatcagga acagttcggc agctggtaag tcaaacacca ggagcaatca gctatttagc
     2581 tttctcatat ctcgatgatt ccacacaagc actaagtatc gacggtgtag aaccaaaaga
     2641 agagaatgta gcagataaca gctggggaat ctggtcatat gaacatatgt acacgaacgg
     2701 aaaaccttcc cctgaagtcc aaaaattctt agactacatg atgacagaag aaatccaaga
     2761 aggacctgtc aaagaattgg gctatttacc aatcacaatg atggaagtag aacgtgatca
     2821 cgaaggaaat atcaaataac tagcagataa acatgccacg gtagtcgtta ctattaagga
     2881 aaaatcaact atgtttcccc acatatacat ttttccttta attgtgaaaa atagttcctc
     2941 atattttgcc gttttttgag ctaataccga gggtcagctg attgaacacc gtttattcga
     3001 aaaaaagagc tacgacatag aactgtcgta gctttttttt tgctatatga caggaatgaa
     3061 aggtgtttgc aagcttaatg tgaggaacac tttccatatc tgttctatac tggataggag
     3121 aagtttggaa atatccagaa aacgaaatca atcgaggtag ccacatgaag aataaatgga
     3181 taaatcgaag taaaaaaaag aaagtaacac aaagatcatc agataaagtc atccgtcttt
     3241 ttccatcgta taaagaagga ttaacggcag atcaagtcaa ggaacgaata gaaaaaggtg
     3301 cagcaaataa ttcagtcgat cccacattca aaacaaatca gcaaatcgta ttggaaaaca
     3361 tatttacgta tttcaatttg atctttctta tcttagccat tttgctttgt ttagtagaat
     3421 cctataagaa cttgactttt ttaccagtta tcatagccaa tacaggaatc gccatttttc
     3481 aggaaattcg atccaaaaag atcttggacg aactaaatgt tttaaataca gcaaaagtca
     3541 atgtcatacg agacgggata gaacaaacaa tagatataga agaccttgta atagatgaca
     3601 ttgttatttt agagactgga catcaaattc cagcagatgc agaggtcgtg gaagggaaac
     3661 tgcaagttaa tgaggcttta ttgacaggcg aagcagatga aataaccaaa gaaataggcg
     3721 atccattaat gtccggcagt ttcattgttt ccggaaaagc ttacgcccga ttagacaaag
     3781 ttggagcaga ttcgtatatt tcccagctga cagtcaaagc caagacaatg ggagaaggag
     3841 aacagtcgga gatgattgct tctcttaatc aattgatcaa atggattggg atcatcatta
     3901 ttcctatcgg tatcctgctt ttttctcaaa gttacttttt taatggaaac acgatcaaag
     3961 aaagtgtcgt tgcaatggaa gcagcattga tcggtatgat acctgaagga ctgtacttgc
     4021 tgacgacgat tgcattagca ttaagtgcga ctaaactagc aaagcaaaga gttcttttac
     4081 acaatatgaa aagcattgag acattggcaa gggtaaatgt cctttgtgta gataaaacag
     4141 gaacgatcac agaaaataaa atgtctgttc aaaaagtgat cgtctctaaa aaacaagaaa
     4201 taatcagtca gtcagatcaa ttggaagcat gcattgctga ctatgcaaaa gcgatggcag
     4261 gagacaatgc aacaatggaa gctgttaaag catattttac aaaaaccact gatcaatcct
     4321 atcaccatgt tattcctttt tcttctgtcc aaaaattcag cagtgtagcg ttggcagaca
     4381 aggtgtatgt tttaggagcg cccgaaatgg ttttgcgcga tcaattgccg gaatatgcgg
     4441 aagagtttgt ttctttcgct gaaaaaggct atcgcgtgtt ggtctttggt gtgtacgatg
     4501 ggattcttga agaaccagta ttgacagaag cagtcacacc attaggctat attctgattg
     4561 ctaatcctat cagaaaagaa gcaagagcca catttgatta cttcaaaaaa cagcaagtag
     4621 ccatcaaagt gatttctgga gataatccgc tcaccgtatc caatgtagct gtccaagcag
     4681 ggatttcaca tgctgatcag tacgttgatg catctaaatt atcagaagaa gaatatgaag
     4741 cagctgtcga aaaatataca gtttttggac gcgtcaagcc agaacaaaag aaaatcttcg
     4801 ttcaattgct aaagcgtcgt aacaatacag tagctatgac aggtgatggc gtgaatgata
     4861 ttctagccat gaaagaagca gactgcagta tagcgatggc ttccggaaat gaagccgcca
     4921 tgcaggcctc acaagtggtc ttacttgatt ctgacttttc acgcatgcca gaagttgttg
     4981 cagaaggacg gcgcgttgtg aacaatattg agcgatcagc cagcttgttt ttagtgaaga
     5041 atattttctc ctttttgctc tcactgtttt cagtgatctt tgctctgacc tatcctttag
     5101 aaccatcaca aatcacattg attagtttgt ttactatcgg acttccatct ttcttgcttg
     5161 ctctagaaga aaacaaaaag cgaatcagag ggaaattcat catgaatgtg atggaaaaag
     5221 ctgtgccagg tggactaaca gatatgatcg tagtcggtgc gttggtgatc tgcggggtga
     5281 ctttgaatct aaataaaaca gatgtttcta cagcatctac catgcttttg attgcagtag
     5341 ggttcctcgt tctctataaa atctgttcac cgctgaataa attcagaagt cagatcattc
     5401 ttttctgtgc tagcggtaac tttttttcgg tcatcttttt gcataagctg ttttcaatca
     5461 ccggtatctc agctgtatca cttttgctgt tgagcattct atttttctcg gcagattcga
     5521 tttttaggca tttgaccaca ttggtagaat atttgtttga acgtaaaaat gaaaaagagc
     5581 aaaagccgtt tagtcgcgta gttaaaaaat ttctatcgat atttaaacga gaaaattgac
     5641 gaaaagtatc tcaaaaaaga gtaaaaaaga acaaccactc gtgtatcttt cgaagcagga
     5701 tgagtcatcc aactttaaaa cgcacgagca gttgctcttt tttacatttt cggaatggta
     5761 acggtgaaag tcgaacctgt tccaagatga ctgtctacag taatcgtacc atgaaggagt
     5821 tgcacgtagt cttttacaat cgctaaccct aatccggtcc cgcctgaatg ccggcttcgc
     5881 gccttgtcta cgcggtaaaa acgctcaaag atccgttcct gatcctcaat gtcgatacct
     5941 ataccaaagt cttggacaga aaacgataga tcatctgttg cctgatagcg gataataatc
     6001 tcgctgtcgc tttttgaata ttggacagca ttttcaatca gatttttgat aattggataa
     6061 aggatatctg ttttagtagt aaatggttcg ttctttccaa tcaatcgaat cgtcaaccgt
     6121 ttcttttcga tgattggctg ataattttga acgatttgct ggaaataagg ttctgtataa
     6181 attgtctgga tctcgtaaga attctcgcca tcttttgaca gctggatgat ttcccgaatc
     6241 agtttatcta accggatcgc atctttttgc atgatctcaa gaaaagaagt cagagtttgc
     6301 gggtcatctt ttgcaccatc cagtaaagtc tcggtaaaac caatcagtga agtcacagga
     6361 gtttttaatt cgtgagagac gttgccaacg aaatcctttt gtagcttttc taaacgtcgc
     6421 actttcgtta agtcatacgc cacccctaga atctgtccgg taccttctga attagtaaag
     6481 tagcgcaaac tcaagtctaa tgtctgccga ccttctgtaa gggtgatctc ctgatggatc
     6541 agcggatgct caggagtcac ttgatgaatc agctgaatga gttttggctc ttgaatgact
     6601 tctgtgtacc gctgttcagg ttgatagctt accacgttta aatgtagctg catctttgga
     6661 ttaagcagta gtaaacgaga atcagcatca tcaatcagga atacgccaat catcagttcg
     6721 ttcagtaacg tatataattg ctcttctgtg gtagtatacg cccgatacat cttactcatt
     6781 tgttcgctca acgcattgat cgtctgatac aattcttccc attgatcggg tgtttgcatg
     6841 attacttccg cctgattagg attttgcacc atttttttta gtacgggcag cacggttttc
     6901 aatggctggt ttttttgata gatcagataa tagatcattc ctgcaagaac aaggaagaaa
     6961 gacagaaaga agaaaaagac ccaacggcga aaactctcag ttctcggtaa aaaaccgctt
     7021 gtcggttctg cgatacgtaa aatggcttct aactggccac tttttttgac tggtaatgcg
     7081 acatataaaa gatccccatt caaagtagta cttttgcgca aagcgctacc taggttccca
     7141 ccattcaata cggctttgat ttcgggacgg gagtttcgct gttcattcaa tgtttcattg
     7201 tttgtgtcaa ataagatctt tccagtagca tccattaaag taatccgttc gttcgactga
     7261 tggacgaatt cttccaaagc agttttgttt tgcgctgctt ggaggttttc gacatctaat
     7321 tgccggatca gcagttgtgc tttttttgtc aggtagcttt cttgctgttc cacgatctgc
     7381 tgttgaaaat aatgggaaat caattgccag ccaccaaaaa acaaactaac taaaagaata
     7441 agaaagaaga gacgttcata aatttttttc catttcatcg tttgggttcc tgaaacttat
     7501 agccaaatcc tctaatagtc accaagtact ttgggtgttt tggatctcgc tcgatttttt
     7561 ctcgcaaatg actaacgtgt acatctacga ttcggctttg tcctgcgaag tcaaaattcc
     7621 aaatgcggtc tagcaatgtg tctcggtcga tcacacggtc ttttcgtttc atgaaataaa
     7681 caagtaattc aaattctttc ggtgttagtt caatcagatg gtcgtctacg gtcacttgat
     7741 aattggtcaa atcagcagtg atttgtccaa tcgatagata ttcaggctca gcttcatctt
     7801 ttgattgctt acgaggttca attcgtctga atattgcttt catacgggct aatacttcgc
     7861 gaggactaaa tggtttggtc aaatagtcgt cagcacctat ttctaggcca atgatccgat
     7921 ccacttggtc atctttagcc gtcagcatca aaatcggtgt ttcatttttt tcttgacgca
     7981 gccgctgagt gatctccatg ccatccatcg ttggaagcat cacatctaaa atgatgaaat
     8041 caaatgattg ttctaatgct aattctaatc cttcgcctcc atctgtggca ctggttacct
     8101 tgtagccttc tttttccaaa ttgaacgtca agagcgttac aatagaaggt tcatcatcaa
     8161 caactagtat ttttttcatt caaatctcct ttaaataggt ttcattctgt ttatatttta
     8221 gcatagaatc agtacaaagg gctattttac aaaagaaata aatggtataa taggaaggca
     8281 ctttgctgaa tagaaaggaa gagcagtcaa tgattggaat cagtgcatgt ttaggcggtc
     8341 tggcctgtcg ctatgatgga cagaaaaaag aagtaccaga attgatggaa ctagttgaaa
     8401 ctggagaagc tgtcatggtc tgtccagaag tgatcggcgg attaccaatt cctagagatc
     8461 ccgctgaaat cattggtgga aatggattcg atgtgtggaa agatcaagca aaagtatgga
     8521 cgacaagcgg cgaagatgta acggaagcgt acaaaaatgg cgcgatcaaa gcctatcaaa
     8581 aattgcagga aaaacacatt gatctagtca tcatgaagga aaaaagccct tcatgcggaa
     8641 cccattcgat ttatgacggg agtttttccg gaataaaaaa aaatggtgcc ggagtggcta
     8701 ctgcttattt tattcagcaa aaaatgactg tgctctctga tgaagaatgg ctgaaaacta
     8761 gaggtgagca gaatggaaat cgaaaagacc aatcgaatga acgcgctctt tgaattttat
     8821 tcgaccttat tgacagaaaa acaaatgaac tacatggaat tatattatgc agatgatttt
     8881 tcattaggtg agatcgcgga agaatacgaa gtaagccgac aagcagtata tgacaatatc
     8941 aagcggacaa gcaagatctt ggaggattat gaaaaaaaac ttcatctttt ttctaattat
     9001 attgttcgag aacaggtatt ggaaaatatg actgcatata ttacagataa gtacccggaa
     9061 gataaaaaac tacttgaata tgtacagcaa attcaagcaa tcgaagaata aataaaagga
     9121 atgaagaaaa tatggcattt gaaagcttaa ctgaacgtct ccagcaggcg atgggaaaaa
     9181 taagaaaaaa aggaaaagta tcagaagctg acgtaaaaga aatgatgcgg gaaatccgcc
     9241 ttgccttact agaagccgat gttaacttgc aagtagtcaa agattttact aagcgcgtac
     9301 gcgaacgtgc ggtaggggca gaagtgctgg aaagtttatc acctgcacaa caaatcgtaa
     9361 aaatcgttga tgaagaattg acgatcacac ttggttctga aacagcagag ttaaataaat
     9421 caccaaaaat accaactgtt atcatgatgg ctggtcttca aggggcaggt aaaacgacct
     9481 ttactggtaa attagccaat tatttaaaga aaaatgaaaa tgctcgtccg ctcctaatcg
     9541 caggggacgt atatcgacca gctgcgatcg accaattgaa agtattaggt caacagctag
     9601 acgttcctgt atttgatatg ggaactgaag tcagcccggt agagatcgtc cgtcaaggga
     9661 tggagttagc aaaagaaaag aaaaacgact atgtattgat cgatacggca ggacgtctgc
     9721 atatcgatga aacattgatg gatgagttga aacaaatcaa agaattgacg caaccaaatg
     9781 agattttgct tgttgtcgat gccatgactg gtcaagatgc cgtcaacgta gctgatagct
     9841 tcaaccagca attaggtatt acaggggtcg tcatcactaa acttgatggg gatactcgag
     9901 gcggggcagc actatcgatc cgctcagtga caggcgcacc aatcaaattc atcggttctg
     9961 gtgaaaaatt gactgactta gaagtattcc acccagatcg tatggctagc cgtatccttg
    10021 gtatggggga tatgctgaca ttgattgaaa aagcgcagca agactatgac gagaaaaaag
    10081 cggaagaact tgcacagaaa atgcgggaaa attcattcga tttcaatgac tttattgaac
    10141 agcttgatca agtgatgggt atgggcccac tggaagatct aatcaaaatg atcccaggaa
    10201 tgaatcaagt ccctggaatc gaaaacgtca aggtagatcc aaaagatgtt gaacgcaaaa
    10261 aagcgatggt ttattctatg acgccagcag aaagagcaaa tcctgatctg ttgaacccaa
    10321 gccgtcgtcg ccgaattgct gccggttctg gaaacagtgt tgttgaagtg aatcgaatga
    10381 tcaaacaatt caaggaatca agaaaaatga tgcagcaaat gtccaaaggg gatatgaaca
    10441 tcccaggtat ggaccaaatg tttggttcag gcgtcaaagg aaaactaggc aaaatggcga
    10501 tgaatcgaat gatcaagaaa aataagaaga aaaagaaaaa acggaaataa gatgaaaaaa
    10561 acagcttcgg tacagactga aaatgtctac tgccggagct tttctatcag caatttttat
    10621 tctttactaa tacaattact ctaaacgaag aggtggcaca gatgagatgt ccgtggagtg
    10681 aacaaacaga aagtatgcgg caatatcatg atactgtctg gggtgttcct gaatatgatg
    10741 accaaaagct gttccgaaaa ttgatgcttg atattaatca agccggattg agctggcaga
    10801 cgattttaaa taaaagtgaa gcattcgatg aagcatatga tcgattcgat attaaaaaag
    10861 tcgcaaatta tggagaaaca aaaatcagtg aactaatgga aaacaaagga attattcgta
    10921 accgaagaaa aatcgaagca gccgtccaaa acgccaaagc agtcttgaaa attcaagaag
    10981 aaatcggcag tttttcagag tatctttggt cgttttctaa tggaaaaatc gttgattctt
    11041 cttatcattc acaagaagaa gtcccaacaa caagtcctct ttcagatgaa ttagcaaaag
    11101 atctaaagaa aagaggattt aaatttatcg gaagcacaac aatctatgct tttttagaag
    11161 ctgtcgggat catcaatgat caccttgaca gttgtttccg caaagaagag gtcgtacgta
    11221 tcggcagaaa gggcgaataa agcatgcgaa aaatcatatt ttatggtgct ataagtttag
    11281 atggctattt ggcaacgaaa acggatgatc ttcaatggct ttttgacact ccaacaggta
    11341 agcaaaccac ttatgaagct ttttatgaaa caatcgatac gaccatcatg ggcagaaaaa
    11401 cttatcagga agcaaaaaaa tatttggaga ccgaagcaat ttatccagag aaaaagaatt
    11461 tcgttttttc cacccacgca caattacaat tgccagatgc tgaggtcgta aacgaagatc
    11521 ctgttgcttt tctaaaaaat ctacaaatga cagaaggaaa agatatctgg gttgtgggtg
    11581 gcggtcgatt gatcaaaccg ctattggaac atcaaatgat tgatgaatgg tatatccaga
    11641 ttacccctgt attattaggg gacgggattc ctttattcca aaaaggaaca tatgaagcgc
    11701 gttttgaaat gctagacacg acacgattcg gtgaatttat cgaattgcat tatgtacgaa
    11761 agtagaagta aataaaataa aaaaggcaac tatatgcaga aatgtcctgt acatagttgc
    11821 cttttttatt accgttatgc tttttactct gttaatttgt tagaaaaatc tagatacgca
    11881 tctaaaatcg tttggaagaa acttaatgtt ccttcaacga tattaccatt ttcatcaatt
    11941 agattcgcaa cgtttccgat atatgcttcc ggttgctgca aggttggcac attcaaaaag
    12001 acaagtgatt gtcgtagatg gtggtttgcg ccaaagccgc cgatcccacc tggtgatgca
    12061 gtaacgacta aacctggttt atgatcccaa acactgtgtc cataaggtct tgagcctaca
    12121 tctaaagcat ttttcaatac tgctggcaca gaacggttgt attcaggagt aacgaaaacg
    12181 acaccgtcta gtcccctcac ttcctcacga aaacgagtcc attcagcagg agcctgttct
    12241 ggagtttcga gatcctcatt gtaaaatggc agatcatcga tttttacaaa tacactttcg
    12301 tacccttcag gaagcaattt cgcaaaagct tctgctactt ttttgttgta agaatctttt
    12361 cttaaactac caataaaaaa tccaattttt tttgtcatac gatttttcct ccttcaaact
    12421 atctataaca atcttacaaa aaataagtaa ctggagcaaa taaaaacact tactgacaaa
    12481 agctacatag aagatagatt acttattttt aacaaaaaag aagtctaaaa tcttctattt
    12541 cctaaaaaaa atcgaattta atcaagctgt aaagaaaaaa agctttacag ggtaaaaaaa
    12601 aactgttaca ctacatcttg tgaataaaac aatggaggtg gaaataaata atggcagtta
    12661 aaatccgttt aaaacgtatg ggttctaaaa agagtccgtt ttaccgtatc gtcgtagctg
    12721 attcacgttc tcctcgtgat ggacgtttca tcgaaactgt aggtacttac aatcctttga
    12781 aagaccctgc agaagtagtt ttaaaagaag acttagttct tgactggtta tcaaaaggtg
    12841 cacaaccttc tgacactgta cgtaacatcc tttcaaaaga aggcgttatg caaaaacacc
    12901 acgaagctaa attctcaaag aaataaggtg accggatatg aaagatttaa gcgacttagt
    12961 cttaacaatc gttcgtccgc tagtcactta tcctgatcaa gtcaagttag acgtcgtaga
    13021 gtcgaaagac ttttacgagt acaatctaac agtggctcct gaagatattg gccgtatcat
    13081 tggaaaacaa gggcgtgttg ctaaagcaat ccgcacaatc gtttacggtg ttcggatcaa
    13141 tgcaccgaaa aaagtgcgtt tgaatatcat cgatgataag gaaaagtaaa taaacgtgct
    13201 gtactcgaat tttcgagtgc agcttttttg tttttttatt aattgaagaa atgattattt
    13261 ggcaaaacaa gcaagaaagc gtatccttat aggaggaggg agtgaacagc caatgagaaa
    13321 gaaaatgtta tttattccat ttgctctatt gttaagcgct tgtagttcgg ttactcaaga
    13381 atcagacaat acacatctat caagcagtat atctgaaact gttaccagtc cagaaaaaag
    13441 tagtaccaaa acggctacca ccactgaaca aacaatggct acctcaaata atgagaaaaa
    13501 gactgcttta gaccagttga aagaacaaca gccaaatgtt cccatgcctc tagatgtacc
    13561 agtctcttca ggctatctca atatcgcagc gacacataca aagcaaggtt attctatttt
    13621 atattacaga acagatcgtc ccctcgggtt gaatgctgac gaattgaatc aagaaacgcc
    13681 tattgcaacc tatctatatc aatatgggtt tgcttctagt caagaaacaa tccaagtttt
    13741 gcagcccttt gagatcgaca caaatggtca gcaagtggac ttaggttctc gaatcacagg
    13801 gtatcagcaa ggtgcagcag gttcaagttt tcttgaatgg caagaaggaa actggcgtat
    13861 tcgaatccgt gggaataata tagaaggcca agatccctta ctattagcta aagaaatcgt
    13921 agcttatttg gaagaaaaca gtctaccagc acctgaacaa tttggtaaaa ttactgttga
    13981 tatgggtgat acgacgaatc gagccgtaga agtaagctgg caagaaccga aaaatgccta
    14041 tacgatcact catcaagatc ctatgtctgc gttgaagatg gcagtttcta tgaagaggtt
    14101 gtgaaatcag cgcgctgatc tgagcccatc atttaactcc aagtcacagg aagtaatcaa
    14161 aaatagcacg ccgcattttt gcataatttt ggcttaatac cttaaaagaa aactagaatt
    14221 gcctcttcac acatgctaaa ataaaacaga caaaaaagaa atggggatat aagttgactg
    14281 aatacttgaa tgtaggaaaa atcgtaaaca cacaaggtat caaaggcgaa gtccgtgtta
    14341 tctcaacaac ggatttccca gaagaacgtt acaaaaaagg aacggtgtta acactattcc
    14401 aagacggaaa agcacctgtg gaattaacag tcaaaagtca tcgcaaacat aaaaattttg
    14461 acttattgag ttttgaagga catccttcta tcaatgatgt ggaaaaatat cgggatggca
    14521 tattaaaagt gtcaaaagac aactctgtag atctagctga aaatgaattc tattaccatg
    14581 agatcatcgg tctgcatgta ttagaagatg gaactgagtt aggtaaagta aaagaaatct
    14641 tgtcaccagg tgccaatgat gtttgggtcg ttcaacgtcc gaaaaaagct gatatattgc
    14701 ttccatatat cgcttctgtc gtcaaagaag tcgatttaga agctggagtg gtccgtgtag
    14761 aaattccaga aggactgatt gacgatgaag attgatgtat tgactttatt tccaagaatg
    14821 tttgaaggtc ctatggggga atccatcatc ggtaaggctg tagataaagg attattagat
    14881 atcaatatct ctaacttccg cgattattca gataacaaac atcagacagt agatgattat
    14941 ccatacggcg gtggagcagg tatgctgctc aaagtccagc ccatctatga taacatccaa
    15001 gccattgaaa aagctgcacc ggatgtcaaa aaaagagtaa ttcttcttga cccagctggt
    15061 aaacgattcg atcagaaaat ggcagaagaa ttttcacaag aagaacactt agtattcatt
    15121 tgtggtcatt atgagggata tgatgaacgt atccgcacat tagtgacaga tgaagtttcc
    15181 ttaggagatt atgtccttac tgggggagaa ttaggtgcga tggtcatgat cgatgcaaca
    15241 gttcggcttt tgccagatgt gttagggaat cagacttcag ctcaaacgga ttcttattca
    15301 accggtttat tggaacatcc ccaatatact cgcccggcag aatataaagg gatgaaagta
    15361 ccagaagtat tgacaaatgg aaatcataaa ttgattgaag aatggcaatt aaaagaatca
    15421 ctaagaagga cctatcaacg tcggcctgat cttttggaaa cacttgaatg gacacctcaa
    15481 atgacgaaat ttttagaaga aatcaagaaa gaagaacaag aaaaaacagc agatgaagcc
    15541 aagtgacttt ttctaaaata ttctttacag taggttgata atatgttagt attttatggt
    15601 gagtgcaaaa gcacttaact attacgatat tccgctatga gaaaagctca taagaatatt
    15661 tggaataagg agaagaaaac catgaatcca ttaatcgaag aattaacaaa agaacaatta
    15721 cgttctgaca tcccagcgtt ccgccctggt gacactgtgc gtgttcacgc aaaagttgtt
    15781 gaaggtactc gtgaacgtat ccagttattt gaaggtgttg ttatcaaacg ccgcggtgct
    15841 ggaatcagcg aaacttatac agtacgtaaa gtttctaacg gagtgggcgt tgaacgtaca
    15901 ttcccattgc atacaccacg tgttgcaaaa atcgaagtag ttcgctacgg taaagtacgt
    15961 cgtgcaaaat tgtactacct acgtgcatta cacggaaaag ctgctcgtat caaagaaatc
    16021 cgtcgttaat cattgttatc agactgagaa agaacccttg gtattcaagg gttctttttt
    16081 cttagcatgt aatttgaaca ccctgatcca gtttttttct ttaattattg gtaggggaga
    16141 ataatggatt agaataaatc tacctataag tagtagtaaa aaaaagagca ctatgataat
    16201 atctttctta agagtaaccg ctttaataaa aaatccagaa tattctaaca taaatggagt
    16261 attcttagaa tcaaaaggag aaagaagatg gtttatactt atacggagca agaaaaaatg
    16321 aaagtttgag acacattcat gaatgatgga aaagtaaaac aagtaccaag ttcaacaaaa
    16381 aagaaaaaca tcctattgaa agaagtcttg aaacgctttg accatggagt gacttataca
    16441 gaaacagagg ttaattcgat actgctgaac gtattttcta gtggcgatta tgtcgaacaa
    16501 agacgttatc taatcacatt tggctttttc aaaagaagtt cagacggtcg ggcatatcag
    16561 atgatgggaa ttgaaaatta gaagttttaa tttatactca ttgaaaaaag ttcaagtatg
    16621 cgatattggt tctacaccgc atacttgaac tttttttgtc atctgtatct ataatacaaa
    16681 gccaagaacg ttattcaaaa tatggatagc tatgataaaa gctaatgagc gattcgcagt
    16741 taaataagca atcgtcagta aaatcgttcc aggagcgtac atgaaaaaat caattggatg
    16801 cgttacatga ataaagataa ttctctttta tttgtacgaa taaataccaa aacgtacctc
    16861 ctaaaattag attcaaagaa gatagcaaag aggaaaaaga tagttctgca gcagctatag
    16921 cggcatgtgg attgctagaa ctttccaaac aactgcctgt ttcagatcct cgtcatttcg
    16981 attacgaaga gatagcaatc aaaatcttgg aagaattaca gaaaaactat acaacgaaac
    17041 gagttaacgg atcaaacggt ctgcttctcc atgccgttta tgataaaaac agcttaaaag
    17101 gtgtagatga atgtgtgatc tggggcgatt acttttatgt agaaggaatc actcgtctag
    17161 ctaaaacatg gtactgttac tggtgatgta taagagaaga ggaggattca tataatgaag
    17221 cgatggaaaa aaatgatgat tggatcaata gttttaggaa tgtcactcgt atttggtgct
    17281 tgtggtaatc aagaagacag tagtgaagaa tcagcaaatg aaattaccgt ttcgacatgg
    17341 aattatgaaa caacacctga attcaaagcg ctctttgaag cattcgaaaa gaaaacggga
    17401 atcaaagtga aagcagtaga tattgcttca gatgattatg atacgaaact caccacgatg
    17461 ctttcttctg gagacacgac agatgtattg acgatgaaaa atcttttgtc gtattcaaat
    17521 tatgctttac gagatcagtt agtcgatcaa acagaacgaa ttagagaatt agacactgat
    17581 gctgctgaag aaacatatga aatgtatgat atcgatggaa aaacatatgc attgccatat
    17641 cgaacggact tttgggtact atattacaat aagaaaatgt ttgatgatgc aggaatcgaa
    17701 tatccaaaaa atctaacttg ggacgaatat gaagatttag caaaaaaact tagtaaaaat
    17761 gacggacaag tttacggagc atatcaacat atctggcgtt cgacaatcca agctattgca
    17821 gctgctcaaa acgaggcgaa tcttgttgaa ccagattatc aatatatgac tgattattat
    17881 gatcgagcat tgaggatgca aaaagaaggc gcgcagatgg attttggcac ggctaaatca
    17941 acgaaagtaa cctatcagtc tcaatttgaa gaacaaaaag cagcaatgat gtacatggga
    18001 acttggtata tggctggcat actcgcaaac aaagaagcaa gtaagacaga agtagaatgg
    18061 ggaattacag ctattcctca gaagaaaaaa ggagaatcgg tcacgacgtt tggctcacca
    18121 acagcatttg ctgtcaataa aaactcaaag aaacaaaaag cggcgcaaga atttattgaa
    18181 tttgcagcca gtgaagaagg agcaaaagta ttagcaggag taggagtagt tccttcatat
    18241 cgtacggatg agattgatca gctttatttt aatctcgctg gaatgccaac ggatgaaatt
    18301 tctaaaaaag ctttttctcc tgatgaaatc aaacttgaat ttccgattga tacgtatggg
    18361 cctgcaatcg ataagatttt acaagaagaa tatgacttag ttttggttgg agatgaaacg
    18421 ccggaaaaag gaacggtaaa tatggagaaa cgtgtagctg cggaaaaaga ataatcatga
    18481 aaggagagaa gccaagagat aaaacactct tggtttcgtc atcgaaatgg agaatagtat
    18541 aaataagaaa gctacaaatc atttgcgtag aaaacgaacg tggatcgctt attcgtttat
    18601 cgccccgaat ttcattggtt ttttcgtttt tacattaata ccaatcctgt tttcgcttgt
    18661 actagcattt atgaaatggg attctttttc aacgccagaa tttgctggac tagataactt
    18721 tcgtaaaatg gccaacgatg agacattttg gatttctttg aaaaatactt ttttatatac
    18781 cgctgctgta gtgcctggca cacttgtttg ttcgttaggg ctagctttac tactaaatca
    18841 gaaaattaga gggatgaagc tttttcgtac gatatttttc tttccgtatg ttacttcctt
    18901 agtagcaatt gctgttgttt ggaatatgtt gtttcatcca acagtgggac ctattaatat
    18961 ctttttaaga cgatttattg aacatcctcc aggttggact tcaagttcaa attgggcgct
    19021 ttgggcaatt gtaattgtca gtatttggcg agggatgggt tattacatga ttctatacct
    19081 tgcaggacta caaagtatcc aaaaagaact atatgaagcg tcttcgctag atggagcaaa
    19141 ccgttggaaa caatttttga atgtgacatt accttcgttg agaccaacaa ccttctttgt
    19201 aacgatcatg cttgtcatca attgtttcaa ggtgtttgac cttgtgcaag tgatgacgga
    19261 tggcggccct ggtcgagcga caaatgtgct ggtttaccaa atctacaatg aagcattcaa
    19321 aaaatttgat tttggttatg cttcagctat ttcacttgtt ttgttcgtaa ttgttttggg
    19381 cgttacggtt gttcaatttt cttggaataa gcgtcgtact taggaggtgt gatatggaag
    19441 agagtgttcg aaatgaaaga aaaaaagcaa aaattcgttc aaaaaacatg ccctttattc
    19501 aatggttggg tatgattctg ctgattgtat tagctttatt gacgatcatt ccttttttat
    19561 ggatggtttc tgcttcattc aaagcaaata atgaagtgtt aacgattccg attcaatgga
    19621 tccctaaaac ttggcattta gagaattatc tggttatttg gtctcgtatt cctttagtga
    19681 cattttttaa gaatacagct tttttaagcg ttgtgatcac gttgattcag ttgtttactt
    19741 ctagttttgc tgtttacggg ttttctgaac taaattttaa agggcgcgat attttatttt
    19801 tagcatatat cggcacgatt gctgtccctt ggcagtctta tatgatcccg cagttcatta
    19861 tgatgagaaa agtgggttta acagatacgt tgtggtcgtt ggtgctatta caagcgttca
    19921 atgcttttgg tgtgttcttg ttgaagcaat attacagtag tatcccagat tctttatgtg
    19981 agagtgcgcg gatcgatggc ttatcagagt gggaaatcta tcgcaagatc attttacctc
    20041 ttacaaaacc ggcgattgcc agtttgacga tcatcacatt cgttaataca tggaacgatt
    20101 atatggggcc attcatttat ttgtcttcta cggaaaataa aacaatccag ttagggttga
    20161 aaatgtttat tgggctattt gatgcagagt acgcattaat catggctgcc tcagttgttt
    20221 ctgttcttcc tgttgctttt atatttttag cgatgcaaaa atattttgta gaagggatcg
    20281 cttcatcagg aatgaaggaa taggaggtga ttatgaaaga gcagcgtttt gacaaaaatg
    20341 tttatatgga attcttttat tggttgtatc aatcattggc aagtagcctt tgtttttggt
    20401 gtgtcaatat cttgtatata gcagctgcgt tccttttgtc gatagatgga aggaatgtct
    20461 tttcctttat tttttcttta ctttttattg gtccaggttg catcgtgatg ataggtatga
    20521 cagaagaatt agcaaaagaa aaaagttttt caccagtacg tttgttcata aagacgtttc
    20581 gccaattttg gctaaaggga tttttttatt ggctatttgc ttggatagtt agtgtgataa
    20641 tgatttttga ttgtttcttt tttatacgtt tttcttatgg aaaatggttg attccgttgt
    20701 ttgttttact tgcttgtttt acttgcttgt ttatcagttt cctttagcat caactgctgg
    20761 tattttcagg tgagaaatcc agcgtcgaaa ccaaatcaag ttctacggat agcgttctac
    20821 tatacgttga aaaaatggta tgtgagttta ctagattttt tactattgac aagtttgttt
    20881 ctcttctttt tcgtaaagcc ccaatggtgt attctactag gtccttctat cgtatttggt
    20941 ttgatttact tcaacaatcg taaattgatg agaactatgg atctgtagac tttataaatt
    21001 gaaactttca attaagaatg acagtttttt atgtaaaaac tgtcattctt tttcattttt
    21061 tgaaacatat ggctaaacaa atgaaataaa acatgctata cttttgaatg aaacaggttt
    21121 agaaactgta tcacttattt gtgattattt ctaactaata attaaaaata aagcataaaa
    21181 aggacgatgt tcaatggctg aaaaaatgaa acaaggttcg aaatctgtag tagaaagttt
    21241 gattaatcat catgttgatt atgtattcgg tataccaggt gcaaaaatcg atggtgtatt
    21301 taatgaatta gaggatcaag gtcctgaatt aatcgtgact cgtcacgaac aaaatgctgc
    21361 atttatggca caagcaatcg gacgtattac aggagagcca ggagtagtta tcgcaacaag
    21421 tggtcctggt gctagtaact tagctacagg tttggtaaca gcgacagcag aaggtgaccc
    21481 agttttagca attgccggac aagtaaaacg aagcgatcta ttgaagttga cccatcaaag
    21541 catggataat gcagcactgt tccagccaat cacaaaatac agtgcagaaa tccaagatcc
    21601 agaaacaatt tcagaagtga ttgccaatgc ttatcgtatg gctaagagtt caaaaaaagg
    21661 cgcgagcttt atcagtatcc cgcaagatgt agtggatgca cctgtccaag gtaatgtcat
    21721 caagccattg agcgatccta aattaggttc tgcttctgca gatgatatcc gttacctagc
    21781 tgaacgcatt agagaagcaa aactaccggt actattagta ggtatgcgag gatcaagtga
    21841 aaaagaaacg ttagccatca ggcaattagt tgaaaagaca gcattgcctg ttgtagagac
    21901 attccaagca gctggagtga tctcacgtga attagaagcg catttctttg gtcgtgtggg
    21961 tttatttaga aaccaaccag gagatatgct tttgaaacga agtgatctag ttattgcaat
    22021 tggctatgat ccaatcgaat atgaagcaag aaactggaat gcggaaaaag atgcgcggat
    22081 cattgtgatc gatgaagcgc cagctgaaat cgatcctttt atgcagcctg aaagagaatt
    22141 gatcggtgat atctcagcca ctcttgattt attgacaggt tcattagagc ctcaacaagt
    22201 gtcagaagat gcaaaagaat atttagcatc tcttcaagca aaattaacgg agcgggacat
    22261 tgttcaatca aaaggagaag caggtatctt gcatccttta gaagttatta acacgcttca
    22321 gtcaaaagta acagatgata tgactgttac cgtagatgta ggtagtcatt atatttggat
    22381 ggcacgccac tttagaagtt atgaacctcg tcatttgtta ttcagtaatg gtatgcaaac
    22441 gcttggtgtt gcattgccat gggcgatttc tgctgcattg gtgcgtccaa atacacagat
    22501 cgtatcagtt tccggcgatg ggggattttt attctctgcc caggatttag aaacggcggt
    22561 acgtaaaaaa ttgaatatcg ttcatttgat ttggaatgat ggacactaca atatggtaga
    22621 gttccaagaa aaaatgaaat atcagcgggc gtcaggtgta gactttggtc cagttgattt
    22681 tgtgaaatat gcagaagctt ttggcgctaa aggaatccga gctacaagtg tagaggaatt
    22741 agaaaaagct ttagaagaag gatttgcgac agaaggaccc gtaattattg atattccgat
    22801 tgattaccgt gataatgaga aattgggaga aacaatctta ccagatcaat tctattaagg
    22861 aagtgtcgac atgactgaaa aaatcttata ccagcacggg acattaggag ctttgatggc
    22921 cggcttgatg gatggaacag aaacgattgc tcatatatta gaaaaaggaa ctctgggatt
    22981 gggaacattg catgggcttg atggtgaagt catcttttta gatggcgtag cttatcaagg
    23041 aagatcagat ggctcagttg cccaattaga tggaagtgaa cttaccccct atgcagcaat
    23101 tacagatttt acgccagata ctagtttttc tgtctcaaaa actgcagatg gagaacaatt
    23161 gaaaaaagac atattagtaa gagaagccgg ggaaaaccta tttttggcag tgaagataac
    23221 tggattattc aaaaatatgc acatccgtat catgccgaag caagaaaaac cttacagaag
    23281 actagcaaaa atttcagagt ctcaaccaga attccaacaa agcaatgtcc aaggaacatt
    23341 agtcgggttc tttacgcctg aattattcca aggagttgct gcagcgggat ttcacctgca
    23401 ttttatcgat gatacacata catttggcgg acatgtaatg gattttgaag tggctgaagg
    23461 aaaagttgag attagccgca tctcgtccct tgtacagcat tttccagttg atgatccgac
    23521 ttttgttcac gaaacgattg attatgctga tttagcagca gaaatccaac aagcagaata
    23581 gaaaaagaca gcgagatgcg ttcaaggtga ttaacctgac gtatctcgtt tttttgagga
    23641 gaaaacctag cgtttacaaa aaaactgagc tataatcttc tccatagatc aaaaaggagc
    23701 ggtttttatc atgtggacag aaaaacagaa aaaagatgca tacgaaattt tattattaca
    23761 gcaacaagga ttattagaag cagaagataa ctggttagca aatttagcaa attcatctgc
    23821 attgttgaat gagactttgc ctgagactgt ctttgcagga tattatttat tcgatggtaa
    23881 tgaattgatt ttaggaccgt tccaagggcg agtttcttgc acacgtatca agatgggaaa
    23941 aggagtttgt ggagaatcag ctgaaaaaca agaaacattg atcgttgatg atgtcaaaac
    24001 ccatgcagat tacatttcct gcgattcagc cgcacaatca gaaatcgtag tgccaatggt
    24061 aaaagaaggt aatttaatcg gtgttttaga tatcgattgc ggagtaacga aagggtatga
    24121 tgaaatcgat caagcatttc tagaaaaata tgtagatctt ttattagcaa attttaatat
//
LOCUS       DAZBTF010000040        23655 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000040 DAZBTF010000000
VERSION     DAZBTF010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.040"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..120)
                     /locus_tag="ACHGNS_001670"
     CDS             complement(1..120)
                     /locus_tag="ACHGNS_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFN6937613.1"
                     /translation="MVFQKPLNNFRYIVYYCHTKIKKNKQKETVTKVLSQSLI"
     gene            299..1960
                     /locus_tag="ACHGNS_001671"
     CDS             299..1960
                     /locus_tag="ACHGNS_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN6937614.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2030..2392)
                     /locus_tag="ACHGNS_001672"
     CDS             complement(2030..2392)
                     /locus_tag="ACHGNS_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFN6937615.1"
                     /translation="MKEQSNEKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFL
                     WSLPFLIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLII
                     AGILIALSAIFMLFSLLG"
     gene            2460..3401
                     /gene="opp3b"
                     /locus_tag="ACHGNS_001673"
     CDS             2460..3401
                     /gene="opp3b"
                     /locus_tag="ACHGNS_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFN6937616.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3402..4445
                     /locus_tag="ACHGNS_001674"
     CDS             3402..4445
                     /locus_tag="ACHGNS_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6937617.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4451..5521
                     /locus_tag="ACHGNS_001675"
     CDS             4451..5521
                     /locus_tag="ACHGNS_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6937618.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5511..6464
                     /locus_tag="ACHGNS_001676"
     CDS             5511..6464
                     /locus_tag="ACHGNS_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6937619.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6476..7486
                     /locus_tag="ACHGNS_001677"
     CDS             6476..7486
                     /locus_tag="ACHGNS_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFN6937620.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7771..8592)
                     /locus_tag="ACHGNS_001678"
     CDS             complement(7771..8592)
                     /locus_tag="ACHGNS_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFN6937621.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8689..8967)
                     /locus_tag="ACHGNS_001679"
     CDS             complement(8689..8967)
                     /locus_tag="ACHGNS_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937622.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9235..11244
                     /gene="metG"
                     /locus_tag="ACHGNS_001680"
     CDS             9235..11244
                     /gene="metG"
                     /locus_tag="ACHGNS_001680"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFN6937623.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11723..12310)
                     /locus_tag="ACHGNS_001681"
     CDS             complement(11723..12310)
                     /locus_tag="ACHGNS_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937624.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12485..13807
                     /locus_tag="ACHGNS_001682"
     CDS             12485..13807
                     /locus_tag="ACHGNS_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFN6937625.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13962..14738
                     /locus_tag="ACHGNS_001683"
     CDS             13962..14738
                     /locus_tag="ACHGNS_001683"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFN6937626.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14731..15300
                     /gene="rnmV"
                     /locus_tag="ACHGNS_001684"
     CDS             14731..15300
                     /gene="rnmV"
                     /locus_tag="ACHGNS_001684"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFN6937627.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15293..16177
                     /gene="rsmA"
                     /locus_tag="ACHGNS_001685"
     CDS             15293..16177
                     /gene="rsmA"
                     /locus_tag="ACHGNS_001685"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFN6937628.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16628..17257
                     /locus_tag="ACHGNS_001686"
     CDS             16628..17257
                     /locus_tag="ACHGNS_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937629.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17381..18490)
                     /locus_tag="ACHGNS_001687"
     CDS             complement(17381..18490)
                     /locus_tag="ACHGNS_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6937630.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18699..19121
                     /gene="mgsA"
                     /locus_tag="ACHGNS_001688"
     CDS             18699..19121
                     /gene="mgsA"
                     /locus_tag="ACHGNS_001688"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFN6937631.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      19308..19406
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            19444..19980
                     /locus_tag="ACHGNS_001689"
     CDS             19444..19980
                     /locus_tag="ACHGNS_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFN6937632.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20020..21783)
                     /locus_tag="ACHGNS_001690"
     CDS             complement(20020..21783)
                     /locus_tag="ACHGNS_001690"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6937633.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21786..23501)
                     /locus_tag="ACHGNS_001691"
     CDS             complement(21786..23501)
                     /locus_tag="ACHGNS_001691"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6937634.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7623 a         4322 c         4800 g         6910 t
ORIGIN      
        1 tcagataagg gactgtgaca agacttttgt cacagtttct ttttgtttat tcttttttat
       61 ctttgtatgg caataataga caatataacg aaaattattt aatggctttt ggaagaccat
      121 ggaaaacctt gtacaaagct attttttctg aaaaattcct tgaaacctca cgaaaaaatc
      181 attattttat gaaaaagaca aaaaaaagtg ttgatttttt aataattatt cattaaactc
      241 ttaaacaact tcagaaaata ttaattattc tgaaaattcc aataaagggg tacgtaaaat
      301 gaaaaagaaa tttacatttg gtttggttgc ggtatgcggc cttgtacttg ctggatgtta
      361 tggcggcagt tcagccacaa gcagtagttc agcaaaaagc ggttcagcag atggaggagg
      421 cgtatttaac ttagtcgtgc ctcaagaaat gcctacagct gacttatcag ttgcaacaga
      481 tacaatcagt tttacagcat tgaataacgt ttatgaagga atttaccgtc ttgatgaaga
      541 cagcaaacca cagccggcag gagctagcga gttggcagaa gtcagtgaag acggattaac
      601 ttacaaaatc aaattacgag aagacgcaaa atggtcaaat ggtgatcctg ttacagcagc
      661 ggattacgtt tatggctggc aacgaacagt agacccagct acagcttctg aatatgctta
      721 cctgtatgcg ccagttgaaa atgcagaaga cattacagct ggaaagaaag ataaatcaga
      781 actagggatc aaagcagttg gtgactatga attagaaatc aaattgacga aacaaacacc
      841 atatttccaa tatctgcttg ctttcccatc atttttccca caaaaacaat ccgtagtgga
      901 agaaaatggg gatgcctatg catctgcaag tgataaagct gtttataatg gaccattcac
      961 attagaagat tttgatggac caggaacaga tacagaatgg acttataaga aaaatgatca
     1021 ttactgggac aaagatacag taaaactttc tgaaatcaaa gtcagtgtag taaaagaatc
     1081 ttctactgca ttgaacttat tcaaagatgg ccaagcagat gacgtgatct tgtctggtga
     1141 gttagcacag caaaacgcaa acgatccagc ttatacttct gttaaagaag cgcggacaag
     1201 ctatatcgaa ttcaatcaac gtgaaaagaa ttcaccattc aacaatgtga acctaagaaa
     1261 agctatttct tattctatcg atcgtgaagc tttagtaaaa caagtactag gtgacggatc
     1321 agttgtctct acaggattga tcccatcaga tatggtaaaa agtccaacaa cgaacgaaga
     1381 ctttgcaaaa gaagctggaa aattagtttc ttatgacaaa gataaagcaa aagaatattg
     1441 ggaaaaagcg aaaaaagaat tgggcgttga ttctttagaa tttgatttga tggcttctga
     1501 tgatgattca tcgaaaaaag taatcgaata tgttcaaaac tcaatccaag aaaacttgga
     1561 tggcgtaaca gtcaaaccaa cacctgttcc attctctgta cgtcttgacc gctcaacttc
     1621 gggtgatttt gacgcagtac taggcggatg ggctgctgac tatgcagacc caagcagctt
     1681 tactgatttg tttgtcacag gtaattcata caaccgtggt cgctggagca atgcagaata
     1741 tgacgaagca gttaaagcag cttcatcaac agacgctggc gatgaacaag cccgttggga
     1801 tgatttccaa aaagcaatca agatcatctc tgatgatatg ggtgtcgttc cggtgtacca
     1861 aaaagcagaa ggtcacctag tgaatgaaaa aatcaaaggt atcgttcacc atgcggcagg
     1921 cgcttcatgg gattacaaat ggacatacgt tgaagaataa tcgttaatac cgtgagcatg
     1981 agaaaaaacg aggctgcggc aaatgtcgca acctcgttct catattcact tatcctaaca
     2041 aagaaaaaag catgaaaata gcagaaagag caattagtat tccggcaatg atcagccaaa
     2101 agctgtacat accgtagctg gcagacgaga gcgtggaaaa ttctggtttt ttctttcggt
     2161 tccttgttcg tgcaagatgg accgatcgct gaaaatgatc gaagaaaccc gaagaaaaga
     2221 cccataaaaa accgccgata atcagaaacg gcaatgacca taagaataga tcattagata
     2281 attgaatgag tgtaaaggaa tggttgatga gattttttat taaaataacc agaatcagta
     2341 gaaaagctac tgcgtagggg atagattttt tttcatttga ttgctccttt atttttggtt
     2401 cattatctaa taatgtagcg aaacatctgt cagaagtcaa aagctagggg gaaacattag
     2461 tgaatagtta tatcaaatat gtattaaaac gtgtcttttt catgatcatc acgctttggt
     2521 taattgcaac aatcaccttt ttcttgatgc aattgctgcc gggcacgcca tatacgaatc
     2581 aggaacgttt gagtcctgaa acaatcgaga tgctaaataa acaagtcggt ttggataaac
     2641 cagttatcgt acaatatggg atttatcttt ctaatttgct tcaaggagat tttggaattt
     2701 ccttccaatt taaaaaccag ccagtcgcac atttgttagc tggaagaatc ggaccatcct
     2761 tgcagttagg gttacaggca attatctttg gaacagtcct agggacgatt ttaggaacga
     2821 tctctgccat gaagcaaaat acatgggcag atacatcttc tacactcgta gcgatattag
     2881 gtcgttccat tccaaacttt gtatttgctg ttttattaca atatatcttt gcaatcaaat
     2941 tacaagtttt gccaatcgca aaatgggatg gctttgtgta cacaatctta ccaacaattg
     3001 cgttggccat gtcgccgctt gctgactctg ctcggtttat ccgtacggaa atggtggaag
     3061 tactgcatag tgattatgtg gaattagcaa aagcaaaagg attgagccgt tgggaaatcg
     3121 cctttaaaca tggtcttcga aatagtttga ttccattgat gactttatta ggaccgttag
     3181 ctgtagcatt gatgaccgga tcactggtgg tagaaaatat ctttgctatt cctggtatcg
     3241 gggaacaatt cgttaagtca atcacaacaa atgattatcc tacgatcatg gctgtaacga
     3301 tcctttactc atttatgctg atttttgtta ttttgatcgt ggatctattg tacggtctag
     3361 ttgatccaag aattcgtgtt tcagaaggga gccgaggata aatggaaatg ataccaaagc
     3421 gttatgaaac agttgcagat atccctgcgg atgaatttct tccattacaa aaaaataccg
     3481 aagaagaacg tgaacagatt actgcacctt ctttgaattt tatccaagat tcatggcgcc
     3541 gtttaaagaa aaacaaagct gccgttattt caatggtttt actattggtc atcattttga
     3601 tctctgtgat cacaatcttt gtttctcctc atgatccaac tgcccaaaac gtggcttata
     3661 tcaatttgcc tcctcgtatt ccgggaatca atatcgatgg attgaatgga aaagcaatgg
     3721 ttggtggtga acttgttgat aaatatgctc aagcaaacgt cccttcaaac gtaaatttct
     3781 ttttaggaac agacggtttg ggacgtgatg tattaagccg cttatttatg ggaatgagaa
     3841 tctcgttgct gatcgcattt attgctgcat tactagatgt aacgattggt gtagcttacg
     3901 ggttggtttc tggtttgtta ggcggacgtg tcgataacgt gatgcaacgt tttttggaaa
     3961 tcttatcagg aattccaaac ctcgttgtca tgattttgat gttggtcgtt tttgaaccag
     4021 gaattttttc aatcgtggct gctatggcca tcacaaactg gataccaatg gcacgtatcg
     4081 ttcgtgcaca aacaatgaaa ttaaaagatc aagaatatgt tttagctgga ttgacattag
     4141 gtgaatcaag aatgaaaatt gcattcaaac atattttgcc gaatatctct agtgtcatta
     4201 ttattcaaat gatgtttagt attccatctg ctattttctt cgaagccttt ttaagcttta
     4261 tcggtttagg tcttacgcct ccgtcagctt cattaggaac gatgttgagc gaagggtaca
     4321 agacattcct gtatctgcct tatctattat ggattccggc agtaacttta tcggttatca
     4381 tgattggctt taacttactt gctgatggat tgcgtgatgc ctttgatcct aaaatgaaag
     4441 agtgaagacg atgacgaaaa ttttagaagt gaaagactta gaaatctctt ttgatacgta
     4501 tgctggaaag gtacgagcga ttcgcggtgt caattttcat ttgaataaag gagaaacatt
     4561 ggcgatcgtt ggggaatctg gaagtggaaa atcagtgacg accagaagca tcatgcgctt
     4621 gctgtcaagc aacgccaata tagatgctgg tcaaatcctc tttaaaggta acgatatcgt
     4681 tggaaaatca gaaagagaga tgcaaaaaat ccgtggtaaa gaaatagcga tgattttcca
     4741 agatccaatg acctctttag atccgacgat gcccattggg aagcaagtcg ctgagtccct
     4801 tagaaagcat aataaagttt caaaaaaaga gggacaaaaa gctgcattag aattgcttaa
     4861 attagttgga atacccgaag ctgagaaacg tatcaacagt tatccgcatc agttctcagg
     4921 aggtcagcgt caacggatcg tgatcgcgat tgcactggta tgttatccgg aaatcctgat
     4981 tgctgatgaa cctacaacag cgcttgatgt gactattcaa gcacagattt tggagctgct
     5041 gaaagaaatt cagacaaaaa tcgatacatc aattatcttt atcactcatg accttggtgt
     5101 agtagcgaat gtcgctgatc gtgtcgctgt catgtatgga ggaagaatcg ttgaagttgg
     5161 aacatcggaa gaaattttct acaacccgca acatccctat acttggggac tgcttggttc
     5221 aatgccgaca ttagatagtg cgaacgatcg attgtatgct attcctggtt ctccaccgga
     5281 tttattgaac ccgccaaaag gagacgcatt ctatccaaga aatgaattcg cgatgaaaat
     5341 cgatgcagaa caagaaccac cattttttga actatcaaaa acacacaagg cagcaacttg
     5401 gctacttgct ccgcaggcac ccaaagtgac accgccagaa gaaatccaac gtcgctgggc
     5461 tatctttaaa gagaaacaaa aacatactta cggtcaagga ggaattgctg atgccaaata
     5521 aactacttga agttaaagga ttgaaacagt atttcaatgt aggaaaaaag aatgaagtac
     5581 atgctgtaaa tgacatcagc tttcacattt atgaaggaga aacattcggt ttagttggag
     5641 aatctggaag cggaaaatcc actactggcc gtacgatcat tcgcttgaat gaacctactg
     5701 atggtgagat attattcgac ggtcaagata taacgaagat caaagataaa aaaggtttga
     5761 cgaaattccg gcatgatgtt cagatgatct ttcaagatcc atacgcttcg ttgaatcctc
     5821 gtatgaaagt cagagatatc attgccgaag gaattgatgt taatggacta gctaaatcat
     5881 ctgaagagcg tgcaaaaaaa gtcgatgatc tattgagaac tgttggattg aatccaagcc
     5941 acggtactcg ttatcctcat gaattttcag gtgggcaacg ccagcgtatt ggtattgcac
     6001 gagcattggc agtaaaaccg cgttttatca tttgtgatga accaatttct gcactagatg
     6061 tgtcgatcca agcccaagtg gtcaatttgt tgcaagatct ccaaaaagaa catcaattga
     6121 cctatctatt tattgcacat gatttgtcga tggttaaaca tattagtgac cgaatcggtg
     6181 taatgcataa cggcttgtta ttagagatgg gaacaagtga tgaaatctat aatcacggtg
     6241 ttcatcctta tacagaaagt ttgttatctg ctattcctct accagatcca gaccatgaga
     6301 gaaaacgcag aagaatcaaa tatcaacctg aaccagatga cggacaagta cgtcaacttc
     6361 gtgaaatcgc accagaacat tttgtctatg caactgagca ggaagtccca tattacgcaa
     6421 aaaaactaaa acgacaaaag gaatctcttc tggttgctaa ctaggaggga catagatgct
     6481 aaaagctttt aaattccgta tgtatccgac agaagaacaa aaacagcaat tgattcgtac
     6541 ttttggatgt gctcgattta cctataacca tttgctgaaa aagcggcaga aaagttggca
     6601 acaaacagga gtagccaatt tttcgctcac acccgctaca ttaaaaaaag aatacccatt
     6661 cttaaaagaa gtagacagtc ttgctttagc aaatgcacag cttaatttgg atcgggcttt
     6721 tcgcaattat ttcaagggac gtgccagctt cccaaagctt aaaacgaaaa aaagtatgtg
     6781 gcaatcttat acgaccaaca accaaacccg tacgatctat ctggaaaatg gctacttaaa
     6841 gttaccaaaa caaaaagaac taatcaagat aaatcagcat cggccagttg aaggatcgat
     6901 tcgctccgct acgatttctg ctcgatataa tgaagaattt tatgtcgcac tactttgtga
     6961 tgtttcgcca gtaaaaaaag aatctttagc taaatggatc gggatcgcct atcatccgaa
     7021 gacattgatc caaacatctc gacctctaga agtaacttta ccaaaatttc accagacaga
     7081 agaaaagctg caacatgcac agcggaaatt gaatgtgaaa gtccgctccg ctcaccatag
     7141 aaagatcagg ttagatcagg caagcaatta tcagaaacaa aagagaaaag taatggatct
     7201 ttatctgaaa caaaagaacc agcgagaaga ttatcttgaa caattatctg ggaaattggt
     7261 caaacaatat gactacttat ttgtagaatc ttttccaaaa gaagaggcac atgctgattt
     7321 ttctattcac gactggcata aattaataac aaaattgcgt tataagtccc aatggtataa
     7381 taaaaaattt cttttgatca ataccgacgg agcagaagaa agcaattccg taagaaagag
     7441 ccaagtagta gaaaaaatgg gcagacattc ggttatcaag ggataaaaaa tgaatgaaac
     7501 tgataggagc gttagggtta gccttgttaa taaaaagtct taatgacttt tgtttctagg
     7561 aatttttctt ttgaagaaaa gctcactaat tatgacaaac caataaaaca atagcagaaa
     7621 aagttcgtac gagctttttc tgctattgtt aattttttat tcagaaagtt tacgaataca
     7681 tcaaaattct gaaacaaaaa acgtaactca caaagagttg tttttgcgtt ttttcttgaa
     7741 tgattaggtc tgaatctttt gctcacaacc ttattcatgg tgccccggct tgagctgata
     7801 gatttttgaa ggacggccga ttcctacagg acgttcaccg atctcatcga aatattggag
     7861 catagctttc ttaaaattgg aatgatcgat cgttttataa tcgatcccta agaatttagc
     7921 aaatactttt cgagcttccg taatcgtgaa atctttacct aaaacttgta gaacttgcgg
     7981 ctcgtgttcc attttattga ctacacggtt gaatgctttc aagatgattt gactatggtc
     8041 aaaagccaaa gtatctttgc cagaagactc tccagtgact aaatcaagcg agatagccac
     8101 ttctccgcta gataaatgga tcttgttact atgacgttct agtgtgaacc agcgtacttc
     8161 cttggcatca tctccggcga ttagcggttc ttctccaata aaggctaagt agctgactgt
     8221 gacgacccag cctcgcggat ctcgattagg cgtactgaat gtatgcagtt gctcaatatt
     8281 ttcttttgaa ataaccacac cagtttcttc ctttgtttct cttaagacgc tttctccagt
     8341 tgactcatcc ttttggacaa agccaccagg caatgcccat gaatttcgat aaggatgccc
     8401 ttttctttgg atcaacaaaa ctttcagctg atcttcttct ttgttatagc acatcagtac
     8461 gatatcgaca gtcaaagagg gcttgtcata ttctggacgt tcctgttttt gataccagtt
     8521 aagaaattct tcttcacttg ctttatgttc ataatataat ttttcttctt ctttagaagc
     8581 aaaacggttc atctgaccac ctccattgac agtaaaagta ctttttacct tattgtagca
     8641 gactctgaaa aaaaaccaag gatttacttg aaaaatctag gttatcattt aaatatgaaa
     8701 aataaatgga agtatgagat tgaataaaaa gaaaagcaga taaaacggca atactttata
     8761 ccgctcttca cgaatggcaa agaagccaca aataacgata tatacgtcaa tgataaaacg
     8821 caaaatccaa gctgctgtca taaaactgtt caaatgccca tggaggttaa aataaccata
     8881 catacgaata taaaaatagc tgaatattaa atgtgctaat gaaagaaaag caatactaag
     8941 tgtaaaaaaa agatgtttct tctgcatgat tgttccagtc ccttactaag atagatagtc
     9001 tttattatac cacgtgtgag gggagaaata aaaagccgtt ttttagtaga tatgaaacaa
     9061 aacaaaaacc cgcatttatt caacctattc atccttttaa aggatagatt gaacgtagat
     9121 agaagtcgtt tatccagtct ttttcatttt gaaaaaaagc taacagcatg ctttttttat
     9181 ggtatactag caaggaaacg aattcgcatc atgcgataaa ggaggaagag ccccgtggca
     9241 gaaaaggaaa ctttttatat cactacgcca atctattatc caagcggcaa acttcatatc
     9301 ggcaactcgt atactacgat tgcatgtgac gcaattgctc gttataaacg gttgatgggc
     9361 tttgatgtgt tttatctcac tggagtggat gaacatggtc aaaaaatcga aacaaaagca
     9421 gaagaactag gtgtaaaacc gcaagaatat gtagataaaa tggcggctga tgtcaagaaa
     9481 ttatggaaaa cgcttgatat cagttatgat aaattcattc gcacgacaga tgattaccat
     9541 aaagcagctg ttcaaaaaat atttgatcgt ttattagaac agggagatat ttacttaggt
     9601 gaatatgaag gctggtactc tgtttctgac gaagaattct ttacagaaac acaacttgca
     9661 gaagtgtaca aagatgacga aggtaacgtg atcggtggta aagcgccaag tggacacgaa
     9721 gtagaattgg taaaagaaga atcatacttc ttccgcatga gcaaatacgc agaccgtcta
     9781 ttggaatact atgaagaaca tcccgaattc atccagccag aatctcgtaa aaacgaaatg
     9841 atcaacaact ttatcaaacc aggattagag gatcttgcag tttcacgaac aacttttact
     9901 tggggagtac cagtcaagag tgatccgaaa catgtggtgt atgtatggat cgatgcatta
     9961 tccaattata ttactgcact aggatatggc tccgaagatg aaagtctatt cgaaaaatac
    10021 tggcctgcag atgttcagat ggtcggtaaa gaaatcgttc gctttcatac gatctattgg
    10081 ccaatcatgc tgatggccct tgatttgcct ctacctaaaa aagtatttgg gcatggctgg
    10141 ctattgatga aagacggaaa aatgtctaaa tcaaaaggaa atgtcgttta tcctgaaatg
    10201 cttgtggaac gttatggttt agatgcactc cgttattatt tgcttcgtgc tgttccgttt
    10261 gggtcagatg gtgtctttac gccagaagat tttgtttccc gtgtcaatta tgatctggca
    10321 aatgatctag ggaacttatt gaatcgaacg attgctatga tcaacaaata ttgtgatgga
    10381 ctcgttcctg actatgcctc acttgtcact ccatttgata gtgaactgtc tacgacggct
    10441 gctaacgttg tcggacgtta ccatgatgca atggaaaaaa tggaattcaa tacagcattg
    10501 gctgaaatct gggtgctgat ttctcgtgca aacaaataca ttgatgaaac agaaccatgg
    10561 gtattagcaa aagacgaaga aaagaaagct gaattgaata gtgtcatgat ccatttagct
    10621 gaatctttgc gtatcgcagc gatattactt cagccaatca tgacagaaac gccagagaag
    10681 atcttcaatc aactaggatt ggactctgaa acaatgaatc ttgaaggatt acactttggc
    10741 gaatttcctt caggaacaaa agtagttgca aaaggtacac caatcttccc acgtttagat
    10801 atggaagaag aagttgcttt tattcaagaa aaaatgtctg aaggtactca aacaaacgaa
    10861 gatacagtca aatgggatcc agaagaaaca gaacttgttt caacgaaaga aaaacaaatc
    10921 aaatttgatg tatttgaaaa agtagaacta aaagtagcag aagtcatcaa ctgtcaaaaa
    10981 gtagaagggg cagataagtt gctgcaattc cgactagatg ctggagacag ccaagaccgt
    11041 caaatcttgt caggaatcgc tgaattttat cctgatccaa gcgaattgat cgggaaaaaa
    11101 gtcgtcattg tagcaaatct gaaaccacga aagatgcgtg gacaaatcag tcaaggaatg
    11161 atcctttctg cagaagcacc agatggctct ttacaagtaa tcgaagcacc aaaatccatg
    11221 ccaaatggct cagaaatcgc atagaaattc aaagggggta tgacactagt tgtgtcatac
    11281 cccctttttc cgaaatttga gaagcaattt tcgtaaattc cttcttatta cttgaagcca
    11341 atcatataat acagaaacag agaactatcc aaaataaatc ctagaaaaag aagtacgagc
    11401 ttcgctgatt tttgttaatt tagaataata ctaataggaa tcgtaaaaac agaaaaaaag
    11461 cataatgata aagggtcggg cagattttaa ttgattcatc tggtactctt ttttattgtc
    11521 cttacattca taaagtttga atgcaatgac tttattacat ctatatttat atctaacagt
    11581 agcgttgatg tgttaattat aagttctatc actttatgtt caattagttt gtgctcttat
    11641 atgtgtgatt gaaaatatag ataaaaaaac aatcatcggc tttgacttcg atgattgttt
    11701 tctttatttt gtaaaaatcc tatcacttga ttttatggta accttttatt tttgtcttca
    11761 aacggcttgg taagagagca gtaagcagtg caaatgggat cgaaaagaca taccagaaaa
    11821 tcgtacgtcc gataccataa tgttgataga gctttgtcaa catcacgtgt aacaagatca
    11881 accctaaaag caaggagcca gctataaacc aatgattatc ccaatgcatg acttcactaa
    11941 gtgccatcac tccaaaagga tagaaataaa aatagatccc gactaaggtc atccgccagt
    12001 tctttgtaaa tgttccgata ttcaataaaa agaaaatcgc tgaaagcaaa ataccgatag
    12061 gcggaaaaag cccaaccaaa aaggaataca acatccccca caagatcatc acaggacctt
    12121 tgatcaatgc aacgattgcg atgaacaaag tcagcaatat tagtttttgt gataacgttg
    12181 caacagtcat taatccaaat aaaattaatg tgaaccaatg ccaatgggac tgctgttgtt
    12241 ctttacggga aaaatcgacc cggttggata aataatctgc taatttcgga taagaacgct
    12301 taaaatccat aaactgttcc tccttcgtgg aaaaggtagg tttagtatac gaaaaataaa
    12361 aaagaaatac atccgtctta agagggagtt tttcactgaa agtggagaaa aaagaatggt
    12421 aaattaaaat attctcagtt atactgaaaa ttataccaaa tttcaaagaa gaaagaggat
    12481 gggaatgaaa ggcaaactaa aacgagaaat caatctgttt ggcgcattag ctaccgttat
    12541 gggaaccgtg atcggtgcag gcgtcttctt caaaactgct gctgttactg ctagtactca
    12601 gtctacaagt ttgacattgc tagcttggct tttgggtggt ttcttaacga tctgtgctgg
    12661 attgacagtg gctgaattag ctacagcaat cccggaaact ggtggagctg taaaatatat
    12721 tgaagctgcg tatggaaaat tgcccagctt tttattagga tgggcgcaaa gcttgattta
    12781 ctttcctgct aatattgctg cattatcgat tatctttgcg acacaattga cgaacttatt
    12841 acaattatct accgattatc ttttgttgat tgcagtgatc accgctgttt ctgtgacagg
    12901 gttgaatttg ttagggacaa aagtcggtgc ttctgtacaa tcagtgacgt tgattgttaa
    12961 attaatacca attgctgtga ttgtcatatg gggattattg accccaggac aaggaactgt
    13021 ccagttattt ccaatcgaag ctggaaaaga tgttactttt gtcgaaggat tgagcagtgc
    13081 gttgttagca acgttgtttg cttatgacgg ctggttaggt gtcggtgcaa tggcagggga
    13141 aatgaaacgg ccggaaaaag atttgcctaa agcaattatt cttggtttga gttttgtaac
    13201 agtcgtttat ctattgatca attttgtttt tttgaaaact ttgccgattg atcatcttgc
    13261 cggtaatttg aatgcagctt ctgaagcatc agacgtcatt tttggaggaa tcggtggaaa
    13321 gctggtcact attgggatct tgatctcggt atacggtgca ttgaacggtt atacactaac
    13381 tgggatccgc gtgccttatg cgatggcttt agaagacgag ctacctttca gtaaacagct
    13441 gacaaagctt tcaaagaagt ttacggttcc ttatgttcct gcggttttcc agttagcagt
    13501 agcttgtatt atgatgagcc ttggttcttt cgacttttta acagacatgt tgatttttgt
    13561 gatgtggcta ttcagtttac tgatttttat cggtgtcttt attttaagaa aaaaagcgcc
    13621 ggaattgcca cgtccatata aagtaccact ttatccaatc gttccgatta ttgcgattct
    13681 cggtgctatt ttcattttag gtatgacgat gatgacgcaa acgaaattag cattgatcgg
    13741 tataggtgtt accttgatag gtatcccagt ctattatcaa aaaaagaaaa aacgctcaga
    13801 agagtaaaat atttcaacaa cagacagaaa aagtacgaac taggtcctct agatcttcgt
    13861 gctttttcta tttcaataaa acaagttctt ctattttgat ttttgctact tctgcaagta
    13921 gtgcttatgt gctaaagtaa gaatgaaaag gagagataaa catgatattt gattctcata
    13981 cccatttaaa tgctgaacag ttcaatgaag atattccaga aacgattcag cgagcacaag
    14041 aaatgggggt aacgaaaatg gcggtcgtgg gcttcgatac gccaacgatt gaaaagtctt
    14101 tgcaattaag ccatgactat ccaaatatat acagtattat cggctggcat ccgacagagg
    14161 ctggcagtta tacgaaagaa atcgaaaata aattacaaga acaattgact ttaccaaagg
    14221 ttgtagcatt aggggaaatt ggcttggatt atcattggat ggaagacccg aaagaagtcc
    14281 aagataaagt atttcgcaga cagattgcta tcgcaaaaga aatgaaccta ccaatcagta
    14341 ttcatactag agaagcactt gaagacacgt atcaaatcct aaaagaagaa gatgttcgag
    14401 atatcggtgg aatcatgcat agtttcagtg gtgattatga atgggcaaaa cgatttcttg
    14461 acttagggat gcatatttca tttagcggtg tcgtcacttt caaaaaagca ttagacgtcc
    14521 aagaggctgc agcaaatgta ccaatcgaac gattacttgt ggaaacagat gccccttatc
    14581 tggcaccagt tccttttaga ggaaagcgaa atgaaccagg ctatactcgt tatacagtag
    14641 aaaagattgc tgaacttcgg aatcttccga cagaagaagt ggctgcccaa acatggacaa
    14701 atgcgcaccg attatttggt ctaagcgaac atggataaga tgaagatcca agagattata
    14761 gtcgttgaag gaaaagacga tacaagaagg ctgcgggaag tagttgatgc agatacgata
    14821 gaaacaatag ggtctgcaat caatgaagag atcctgatgc aaattgaaca tgcccaagag
    14881 actcgtggag tgatcgtttt taccgatcct gatttttcag gagaaaaaat aagaaaaatc
    14941 attatggaag ctgttcctca ggcaaaacat gcttttttgc cgcgcacaca agctaaacca
    15001 aagaaaaagg gaagccttgg tgtcgagcat gcaagtgatg ctgctatttt agaagcattg
    15061 aaaaaggtcg tgacgccaga catttcccaa acggctgttt ctgaaatcac tagacaagac
    15121 cttttagact atggtttaat agctggggca caagcaaaag aaaaacgaga aaaactaggt
    15181 gacgagttgc gcatcggtta cacgaacggc aaacaattgg aaaaacgttt gaagatgttt
    15241 cgtattacgc cgaaagaact cgcccaagca atgaaaatag tggaggaaca acatgagtga
    15301 atatcgtgaa attgcgacac cctctagaac aaaagagatt ttattgaaac atggtttttc
    15361 atttaaaaaa agtttaggac aaaattttct gactgaacca aatattttaa ggaaaatcgt
    15421 agaaactgcg caaatagatg aacatacgaa cgtgatcgaa gttggaccag gaatcggtgc
    15481 attgacggaa caattagcca agcatgcaaa acaagtcgta gcatttgaga tcgatgatcg
    15541 tttgatccct gtattggctg atacgatgca accctatgac aatgtgacta ttgtacatca
    15601 agatatttta aagaccgatt tgtcaacagc tgttagagaa acatttcatg aggagcttcc
    15661 gttaaaagtc gtagcgaatt taccttacta tattacgaca ccgatcatga tgcattttct
    15721 tgaatcagat ttggttgtgg atgaactagt cgtcatgatg caaaaagaag tagctgatcg
    15781 tatctctgct gaaccaggaa cgaaagctta tggttcatta tcgattgctg ttcaatatta
    15841 tatggaagcc agtttagcat tcatcgtacc aaaaacagta tttgtccctc agccaaatgt
    15901 agattcagcg attttgaaac tgacaagaag agatataccg gcagtcgaag tgacagacga
    15961 aaaagcattt ttccgattga caaaagcggc atttcagcaa agaagaaaaa cgctttggaa
    16021 taacttgcag cattcgtatg gcaaagatga ccagacaaaa gcatggttag caaaaagctt
    16081 ggaaacagct ggaatcgatc caaaacgtcg tggggagact ttgtcattac aagaattcgc
    16141 cgcattaagc aatgcgatgt cagaaaatat gcagtaaaaa aagaggttgt gacagaagtt
    16201 gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa acaaaaatag ttcctcatgt
    16261 ttttcgtttg tttgggctta ttaccgcaga tgcaagcttt tgaacaccgt ttaagaaaga
    16321 ggttgtgggc ctgccgttta actctaagtc acaaggaaca atttcaaaaa acagcttctc
    16381 atgtttttga gttagttggt cttattactt gatgcaagct tttgaccact attatgccga
    16441 acaaggagat gtgacacaaa ttttgtcaca tctcctattt ttgtttcttg atcaaaaaat
    16501 tcttatactt gatactcaca ctcattttgt atggatttag aaaagaatat gtgtcttgac
    16561 agttattagt aagtgagtta tactaagtcg acatttatgt gtcaagacat attaggagag
    16621 agaaaaaatg agaaaaaatt cagttcgaaa actaacgatt tctgctttat tgattgggat
    16681 gggggtcatt atcccaatgg tcatgcctaa aatcatgatt ccaccagctt cctttacttt
    16741 ggcgtctcat gtgccattat ttattgcgat gttttttaca ccaggagttg cagtggctgt
    16801 tgcgctaggt acgacatttg gtttcttttt aacaacgccg gttatcatcg cattgcgagc
    16861 attgtcacat cttgtgtttg cgttgattgg ggcttggtac ttgcaaaagc atccgagtat
    16921 cgttttaaaa aatggacagt ttacttttgc taattggcgt ttccaagggt tcaattttgt
    16981 gatcggtgtg attcattctt tagcagaaat gctagtagtc agcatctttt actttattgg
    17041 taatatgcct gaaacatatt attctcaagg atatttttat acagtattca tcttgatggg
    17101 gattggtggg cttatccaca gtttagttga ttataatatt gcttatttta tagcaggaac
    17161 actcagcaaa tattttgata tccccgtatt tacggcagca aaaaaagaaa atcggaaaga
    17221 gtcgattcaa gggtacgcga agaaaacgac atattagaca tgatctctca aaaaaaaaac
    17281 tctcatctag taatggataa taaatcctct aagatgagag ttttttgtcc taaaaagagc
    17341 tgcaccaaca tcttctggca cagctctaac agggggcaat ttatggaagt ttgatgactt
    17401 gttctgtttc tttatcaaag aagtggcctt tgctgatgtt gaaggcaagg tcgactattt
    17461 cgcctggttt atggaaatct cttgcatcta cttttgagat gaactcagta gaaccagttt
    17521 ttgtgtaaag catcgtttca gctccaagta attcggaaac gacaacttct gatttcacta
    17581 cggcttcagg tgaagcttca agggctactt gttcactgtg gatgtcttct ggacgaatac
    17641 cgaagataac ttcttttcct tcataacctt tttcaactaa tactttgttt tttccttcag
    17701 gaatacgaag cttaagccca tgtccatctg tgataacacc atttttcaat gtcacattaa
    17761 agaagttcat ggcaggagat ccaataaagc cggcaacgaa catattgacg ggtgtatcat
    17821 aaacttcttt cggtgtaccg atttgttgga taaatccgtc tttcatgata acgattcggt
    17881 cagccatagt cattgcttct gtctgatcgt gagtaacata gatggtcgtt gtttcaagac
    17941 gctggtgaag tttggcaatc tcagcacgca tggctacacg taatttagca tctaaatttg
    18001 ataatggctc atccattaag aagacttttg catcccgaac gattgcccgt cccaatgcca
    18061 cacgttggcg ttgtccacca gataaagcag ctggttttcg tttcaagtat tctgtcagac
    18121 ctaagatctc tgctgcattg tccacacgtt ttttgatgtc tgctttatca tatttacgta
    18181 atttcaagcc aaaagccata ttatcaaaaa cagtcatatg aggatacaat gcgtagtttt
    18241 ggaaaaccat ggcaatatca cggtctttag gtgccacgtc attcatgact tttccgccga
    18301 tatttagttc tccttcagag atatcttcca gtcccgcgat cattcgcaaa gtagttgact
    18361 ttccacaacc ggaaggacca acaaaaacga taaattcacg gtctttgatt gatagattaa
    18421 aatcagttac agaataattt tctgcattgt catatttttt atatacgttt ttcagagcca
    18481 tttctaccat aatacgttcc acactttctt ttttagtttt attaactatt taaagtataa
    18541 atgaaagcgt taactaactt caatgtcatg ttgaacaaga attttttctt ttttttgtca
    18601 atgtgctagt tttaagcttt aataaatatc cataagaaat aaaaactgat acactggagt
    18661 aaataagtat gttaacaata tcaaaaggag tgtctcttat gaaaatcgct ttgatcgctc
    18721 atgaccgaaa aaaacctatg atggtgaaat tagcaacagc gtataaaaca atattggaaa
    18781 aacatgaact ttttgctacc gggacgactg ggcagaggat cacggaagca accggcttgc
    18841 cgatccatcg gtttaaatcc ggaccgttag gcggggatca gcaaattgga gccatgattt
    18901 cggaggatca gttagatttg gtgatttttc ttcgagaccc gctggctgca cagccccatg
    18961 aaccagatgt aacagcactt atccgtttat ctgatgtcta tgagattccg ttagctacga
    19021 atatcggaac agctgaggtg ctactaagag gactggaagc cggcttccta gattttcgaa
    19081 gaatcgtcca tgaaatagac aagcatccac tttcattttg atggataagc gtatggaaaa
    19141 ataacgttta atgttcgcct atatgtagct ataaaagcta aacaaactga taatatgcga
    19201 tataatacga acgtattgga taatataaac aataatatta gtttttcgta tttgcagtaa
    19261 atgtgaaaat atgctatagt ttctgtggga aaagatccca aaaataatac agagtagaaa
    19321 ataaagcgtt aagtgctaag aggatgggat gttgcctctt ggacgaagga cgtaatgttc
    19381 gcggttttat tttcgcattc gctgcatgat acgatgtagc agctcttaaa ggagatgcta
    19441 aaaatgaaga aacaccggtt agacgcacgt atgattgcta ttatgggctt attgattgca
    19501 ttgatggtca ctttgtcccg actggtagca atcgagaccc catttattaa aatcagtgtc
    19561 acatttattc cacaagtgat catgggcatt ctttttggtc ctttttggtc agggataggc
    19621 gcagttttag ctgatttagt aggaatggcc ctcttctcga aatctgcatt ctttattgga
    19681 tttacattga atgcatttat tgaaggagca atctacggat tctttttcta tcgaaaagag
    19741 atcacttgga aaaatgccat tctggctacg cttagtgtca cgctgatcat caacttattt
    19801 ttgacaccat tatggcttgc gttgatgtac catgtgccat tgttcagctg ggtcgtttgg
    19861 gcgccccgcc tgttaaaaac agtaatatgg ttaccgattc aatcaattgc catttattac
    19921 gtaggacgta gtattcctta caaaaaaatt ttaagaagtt tagcgatcca cgctaaataa
    19981 aaaacaagca gtcttcccat cccagaagac tgcttgtttt tattgtgctg tgaattgtgc
    20041 ttgatagagc cgtcggtaat aaccatcttt ttgaagcagc tcatcatggg tgcccatttc
    20101 gacaatagct ccctgatcca tcactaatat ctggtccgca tttttgattg tggacaggcg
    20161 atgggcaatc acaaaactgg tttttccttc catcattgct aagaaggctt gttggatttt
    20221 tttctctgtc aatgtatcga ctgagctggt agcttcatct aagatcaaca tagggggatt
    20281 actgatcatc gttcgagcaa tcgtcaacag ttggcgttgt ccatctgata tctttaaccc
    20341 ttgaccgcca atcttagtat ctaatttttc cggcaaacgt tctacaaatt catacatata
    20401 agaggctttt agggcatcat tgatttctgc gtcacttgct tccggattcc caaaacgaag
    20461 attttctcgc aatgtactgt caaatagcca agtatcttgt aacaccattc caaaactttt
    20521 acgtaatgta tcacgttgga tagagcggat gtcatggtca tcgactgtaa ttgttccttt
    20581 tgttgtgtca taaaaacgca tcagcaaatt aacaagtgtg gattttccgg cccctgtttt
    20641 tcctactata gcaatggttt gacctggata agcagtaaaa ttgaaatctt cgatcaaacg
    20701 ctgcttgggg tcataagaaa aagaaacatg atcaaaagag acttttcctt tgatattttt
    20761 gggttccaag gcataggcat ctggcaaatc aggcttttct tctggctgat cgatcaaatc
    20821 aaatgctcgt tctagaccag aaaaagcggt ttgcagttgt gtcgtgattc cagaaagttc
    20881 gataaatggt ttagaaaact gacttgagta gatcgtaaag ctggaaatca caccaacagt
    20941 gattgacgaa ccactcttca atgccaatag gccgccaact aaaccaatcg ataaataagc
    21001 aaggtgatct acaaaacgag aagacgggtt tgtcaaggaa gaagaaaatt gtgcccgttg
    21061 tcctttttca tatagatcag catttagcag ttcaaattgc tgttgactag cttcttcttg
    21121 ctgaaaagct ttcacgattt tctggttacc gattcgttcg gtcacaaagc ccgagatatc
    21181 cccaataatc ctttgctgtc tatcgaaatt tgtttgtgaa gcacgagcaa cgatccaact
    21241 cacaaggaaa ataattggtg tagaacaaag aacgaccaat gtcagaagtg gacttagacg
    21301 catcataaaa ataaacgaaa gaatgatgat tgccactcca gaaaataatt gattgaaggc
    21361 cgcagaaatt gcaatggaaa tattgtccat atcatttgta aatcgactga cgatatttcc
    21421 gtgggagttt tgatcataat aacgaagtgg caatcgattc agatgagcaa atgcatcttt
    21481 tcttaatttt gcaacggata agtaagctaa ccgattgctt aacacttgga tgagccattg
    21541 actgactacg ttgattaaaa cgataccacc gaatagaata agtaaatgga acagtcgatt
    21601 gaagcggacc tgtccttctc caatcatttg atcgatactt tgaccaattt gtaacgtcat
    21661 cacaactgtt gcaatcccat tgatgattcc tagtatcaca gctagccaaa tgtcttttgc
    21721 ataggcagta aggtaaggaa tgaatcggcg gagcgtctgc aaggataatt tttttacttt
    21781 cataactatt cttcctcctg tgatgcgacg atttcctgat attctgctga ttgagcaagt
    21841 aattcttcat gggtaccctg agcagcaatc ttaccttgat ccatcactaa gatatgatgt
    21901 gcttcttgaa tagagcggac acgttgggag ataataatca ctgtactgcc aagataagtc
    21961 tgtagcgcct tacgcaaatc caagtctgtt tgataatcca acgcacttaa agagtcatct
    22021 aaaatcagta atgctggctt ggcaatcaaa gctcttgcaa tcgtcagtcg ctgtttttgt
    22081 ccgccagaga aatttttccc attttccatg acttttgtat ctaaaccatt tggaagctga
    22141 cggacaaatt cttctgcctg agcgattttc agagcttccc aacattcttt ttctgtggcg
    22201 tctttcttac cccattgaag gttttcacga atagtcccgg taaatagtac agaagtttgg
    22261 ggaacaagag caatttgctg acggagatga tccagctgcc aagaacgaac atccattccg
    22321 tccattataa ctgtaccgga actgacatca tagaaacgcg gaatcaattg tgtcaaagtc
    22381 gttttcccgc ttccggttgg accaatgatt ccaaggactg tatttcgctt cagctgaaat
    22441 gtaatatttt ggagagaaag accagaatca ggagtataac ggaagtcaac atggtcaaaa
    22501 gcgataattg cttcagaaga aaaatcggga gtagtactga cctcagcttc aagaatcgaa
    22561 ttttctgtgt ctagcacctc tttcacccga tttcctgaag cttcggcacg tgtgaagatc
    22621 acgacgaggt ttgaaacaac gatcagtgcc aaaagcattt gattcatata attgatcaaa
    22681 gcaagaactt gtccttgttg caagctacca atatttactt tgatcccgcc aactgtcagc
    22741 aaacagatga tcccgacatt catgatcaag gttgtagcag gtgataatag ggcagagata
    22801 ttggaaacac gtaaataatt gttcgctaga tcatttgtgc ttttatcgaa cttctctgtt
    22861 tctttttttg tctgcgcaaa tgcgcggatg acacgaacac cgctaagatt ctgactaacg
    22921 tgttcattga gctgatctag tttttcttgc actttttggt agagaggaac ggtttttttg
    22981 atgataaaaa ataaaatgat tgagaaaata ggcaataatg ctaagaaaaa cagacctact
    23041 tcccagtcga tcacaaaagc catgaccact gacccgatac ttaaaaacgg tgctctgatc
    23101 aataaacgaa tgaccattgc taaagctagc tgaagctgat tgatgtcgtt tgtcattcgg
    23161 gtgatcaagg tatctgttcc aaaatgattc aactcgttgt gagagaaagt attgattttt
    23221 ttgattagct gattacgtaa ctctgtacca aatccttgtg aagctactga agcatagtat
    23281 tggcaaatga tagcacaaat cagaccaatc acagacatag cgaccatcca taagcccatt
    23341 tcaatgattt ttcctcggtc attcattttt agtccattat cgatcaagga agccatcatt
    23401 aaaggcagga caagttcgaa gacagcttca agaaatttga agaacgggcc caaaatgatt
    23461 tgcttacgat agtcttttgc ataacgtaat aactgaatca tatgattcct cctattattt
    23521 atttgtccac aattgaaaaa acaagtacta ccacattgta gcttaggaaa tttagaatga
    23581 aaagagaaca agccttttca taaatgaaag ttttattatg aagaagcttt agaaattgaa
    23641 tagactattt ttttt
//
LOCUS       DAZBTF010000041        23434 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000041 DAZBTF010000000
VERSION     DAZBTF010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.041"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(270..1766)
                     /gene="lysS"
                     /locus_tag="ACHGNS_001692"
     CDS             complement(270..1766)
                     /gene="lysS"
                     /locus_tag="ACHGNS_001692"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFN6937635.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1877..2881)
                     /gene="dusB"
                     /locus_tag="ACHGNS_001693"
     CDS             complement(1877..2881)
                     /gene="dusB"
                     /locus_tag="ACHGNS_001693"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFN6937636.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2882..3769)
                     /gene="hslO"
                     /locus_tag="ACHGNS_001694"
     CDS             complement(2882..3769)
                     /gene="hslO"
                     /locus_tag="ACHGNS_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFN6937637.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3986..6097)
                     /gene="ftsH"
                     /locus_tag="ACHGNS_001695"
     CDS             complement(3986..6097)
                     /gene="ftsH"
                     /locus_tag="ACHGNS_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFN6937638.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6187..6732)
                     /gene="hpt"
                     /locus_tag="ACHGNS_001696"
     CDS             complement(6187..6732)
                     /gene="hpt"
                     /locus_tag="ACHGNS_001696"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFN6937639.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6732..8177)
                     /gene="tilS"
                     /locus_tag="ACHGNS_001697"
     CDS             complement(6732..8177)
                     /gene="tilS"
                     /locus_tag="ACHGNS_001697"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFN6937640.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8297..8767)
                     /locus_tag="ACHGNS_001698"
     CDS             complement(8297..8767)
                     /locus_tag="ACHGNS_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFN6937641.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8813..9238)
                     /locus_tag="ACHGNS_001699"
     CDS             complement(8813..9238)
                     /locus_tag="ACHGNS_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFN6937642.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9305..9574)
                     /locus_tag="ACHGNS_001700"
     CDS             complement(9305..9574)
                     /locus_tag="ACHGNS_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFN6937643.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9585..11180)
                     /locus_tag="ACHGNS_001701"
     CDS             complement(9585..11180)
                     /locus_tag="ACHGNS_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFN6937644.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11195..14716)
                     /gene="mfd"
                     /locus_tag="ACHGNS_001702"
     CDS             complement(11195..14716)
                     /gene="mfd"
                     /locus_tag="ACHGNS_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFN6937645.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14733..15293)
                     /gene="pth"
                     /locus_tag="ACHGNS_001703"
     CDS             complement(14733..15293)
                     /gene="pth"
                     /locus_tag="ACHGNS_001703"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFN6937646.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15646..16620
                     /locus_tag="ACHGNS_001704"
     CDS             15646..16620
                     /locus_tag="ACHGNS_001704"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFN6937647.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16790..18190
                     /locus_tag="ACHGNS_001705"
     CDS             16790..18190
                     /locus_tag="ACHGNS_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFN6937648.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18268..18990)
                     /locus_tag="ACHGNS_001706"
     CDS             complement(18268..18990)
                     /locus_tag="ACHGNS_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN6937649.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19069..19575)
                     /locus_tag="ACHGNS_001707"
     CDS             complement(19069..19575)
                     /locus_tag="ACHGNS_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFN6937650.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19698..21497
                     /locus_tag="ACHGNS_001708"
     CDS             19698..21497
                     /locus_tag="ACHGNS_001708"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFN6937651.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            21991..23379
                     /gene="ltrA"
                     /locus_tag="ACHGNS_001709"
     CDS             21991..23379
                     /gene="ltrA"
                     /locus_tag="ACHGNS_001709"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFN6937652.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
BASE COUNT         6646 a         5056 c         4323 g         7409 t
ORIGIN      
        1 ttttgttatg tttttgaata aatattttct tctgtctttt ggacaagtaa taatatacca
       61 actaaattta gcttggtcaa cgaatttttt gtattttttc aagttttttt caataaacga
      121 ttattcataa cttttctttc tttatcgttc gttttcgtct tctttaatgt ctagaatagt
      181 cggcattagc ttttgaactt tcgttattat ttattccttt ctacacagca aaagctgcct
      241 aaggaattcc ttagacagct tttattcgat tatctcattg ttgggaatag caaaacatcc
      301 cgaattgatt gtgcatcagt tagcaacatt actaatctgt cgatcccgat gcctagacca
      361 ccagttggcg gcataccgta ttctaaggct tccaagaagt cttcgtctac accgtgagct
      421 tcgtcatttc ctagttcacg ttctttttct tgcgcttcga atctttctct ttgatcaatt
      481 ggatcgttca gttctgtaaa ggcattagca aattcgcgcc ccactatgaa tagttcgaat
      541 cgatctgtga atcgaggatc ttctggattt ttcttcgcta aaggcgaaac ttcgactgga
      601 tgtccgtata caaacgtcgg ttgtgtcaat gtttcttcaa caaatgtttc aaagaattca
      661 ttgataacat gtccgacacc catagcttcg gttacttcta cattgtgttt ttcggctaaa
      721 gctaatgctt cttccaatgt catttcttgc cagaagtcta caccagtttg ttctttgatt
      781 gcatctacca tatggatgcg tttaaagtca ctttctaagt cgacagcttg tccatcatat
      841 gtgatttttg ctgttcctag tactttttca gctgcgttac ggataatacc ttctgtcaga
      901 ttcattacat cattgaaatc agtatatgct gtgtatgctt ctaacatcgt aaattctgga
      961 ttatgtgtag tatcaattcc ttcattacgg aaaacgcggc cgatttcata aactttttcc
     1021 attccgccaa caattagtcg ttttaaatga agttctagcg caatacgcaa atataaatca
     1081 atgtccagtg cattatgatg cgtgataaat gggcgtgcgg cagctccacc tgcctcattg
     1141 tgcaatactg gtgtttctac ttcaatatag ccattgccat ctaaatagcg acggatttcg
     1201 ctgataattt ggcttcgttt cataaatcgg tcaaagcttt cgcgattact gattaaatcc
     1261 aagtagcgtt gtctgtagcg ttgttcaata ttcgttaagc catggtattt atctggcagt
     1321 ggacgaagtg cttttgacaa aatcgtgatt tctgaagctt tgatagaaac ttcgcctgtg
     1381 tctgttttca tgatttgtcc agttacacca aagaaatctc caaggtctgc atgtttgaat
     1441 acttcatagg cctcatcacc tacttggtct tttcttacat aaatttgaat ttggccttca
     1501 cgatcttgta gatgcgcaaa tccagctttc ccttttcctc ggcgagtcat catacgacca
     1561 gctacactag cggtcaatcc catttctgct agttcttctt ttgttcttgg atcaaatagt
     1621 tcatgcaatt cttctgagtt gtgcgtgcgt tcaaatcgtt ttccaaatgg atcgattcca
     1681 tcttcacgca attgttccat tttttgacga cgtacaagca tttgatcatt taaatcttct
     1741 acttgatgtt gttgttcctg tgtcacaaaa gttcctccat tctcatgtta atctctttcc
     1801 tataatgcca atgaaacaca aaaaaagcaa gccgaacttg gaatttcggc ttgaaaattc
     1861 acgaaaaact tagaaatcac aacgtttgtt tcaatagtac tttttccgct ttttctacaa
     1921 attgatccag caggtcattg atttcttgct gtttttctgt ctgattgatt gctactttca
     1981 ctttcgctgc tcgaggaatc ccttttaaat aataagccgc atgtgttctg aattctctac
     2041 aagctacttt ttctcctttg agatctgcta agcgttgtaa atgaagttta gctgtagcaa
     2101 ttttttcggc tggtgttggt tcaggaatca gttcccctgt ttcgagataa tgctgtgtgc
     2161 ggtgaatcat ccatggattg cccagcgctg cacgaccaat catcacaccg tctgcaccga
     2221 catagtcaag catccatttt gcatcttctg gcgttttgac atccccgttc cccataaatg
     2281 gaatcgtcaa atgttttttc acttctttca atacttccca gtccgctttt ccttcataca
     2341 tctgaacccg tgtacgtcca tgcatagcga tggcacttgc tccagctgat tgtgctgcta
     2401 atgcattttc tacagcaaag acgtgctctt catcccaacc aatacgcatc ttgattgtta
     2461 ctgggatatt tactgctgaa gaaacagctt ggaccatttc gtatactttc tcaggatcta
     2521 aaagccattt tgctcctgct tctgctttga ttactttatt tactggacag cccatattga
     2581 tatctattat atcggcagct gtgttttctt cgacaaattg agcagcttca actagactat
     2641 ctttatttcc gccaaatatc tgtaaactta atggatgttc tgtttcttca atgtggagca
     2701 tctctaaagt ttttttgttt ctgaaatgaa taccttggtc actgatcatt tcacatacga
     2761 ctaagcccgc accaaattct ttgacagtga ctcgaaatgc tgaatttgtt attccagcca
     2821 tcggtgcaac aacgacacga ttagggattt ccacattgcc aattttccat gtgtgttcca
     2881 cttaaatccc tcctaaggct tctttcttca atccatctaa ttcttcttcg ctatacttgt
     2941 atttgttatt acagaaagaa caaaccgctt ctgcaccgtg atcttcgtct atcattgctt
     3001 ggatttcttc tgcacctaac gcgataattg cttctgcaaa tttttctttt gaacagtcac
     3061 atttgaattg aacaggcatt ttttccaaaa tgttcaattc ttctgtacca agcaatcctt
     3121 tcaatatttc ttctggtgtc tgtccttcat ctaagagggt agagattctt ggtgtcgttt
     3181 ttaattgttc ttcgatcttg ctgattgttt cttcatcggc acctggcatg atttggatca
     3241 tgaatcctcc cgccgtcttg atgctgtcgt cagtattgac caatacactc agtccaacag
     3301 ctgaagggat ttgttctgag atagcaagat agtaagtaaa atcttcagcg atttctccag
     3361 aaacaatcgg cgtttgccca gaaaaaggtt cttttaatcc taagtctttt gtgacagtaa
     3421 acattccttg tgttccaact gctccccgca catcgatttt accttcacta tttagaggaa
     3481 gactgagatg cggatttttg atgtaacctt tcacatctcc ctttccattt gcatctacta
     3541 caattcctgt tgctggacca tcgccttgga ttcgaacagt tagtttgtct tctcctttga
     3601 gcgtcgcacc aagtagcaga ctaccgacca atgtccgtcc caaagcagct gaggaggtgt
     3661 gccaagtatc atgacgtctt tgtgcctccg ctacggtatt tgttgcttga acggcgtatg
     3721 ctcggacgaa tccgtcgtga gcaagtgcct taaccaaata atctttcatt tgttatgtac
     3781 tcctttttcc gtttttaaac tcatagtgaa tcatactagc aactactgct tttttcaact
     3841 ttggaatgct tcttttaaaa ataattcaga atcatctaga aaacaggaca tcaccctatt
     3901 tacaacttaa ataaagaaat ctcccgaaaa ccggtttttg ttccggtttt cgggagtcat
     3961 atcagatcaa ttgacctata tttttttatt tgtattgatc gtctgaatga ttatcttctg
     4021 attgttgaga atctgaatca tgttctgctt tattttcctc attcagttca tgttttgctt
     4081 cttcaaaatc acgtttttct tccgcttgtt tttcagcgtc tttttcttcc aaagcacgtt
     4141 ttgcttcttc aaaagttttc gcttcttttt cgcttggata atcatttgac tcagtacctt
     4201 caggcatcac accgtgttca aacaatgact tgattgcttt ggcatccaat gtttcgaatt
     4261 ccaaaagttt ttcagcaatc agtttatgct gtgcacgatg agcttcgatg atttctcgag
     4321 ctttatcatg agcttccatc aagatttttc gtacttcttg atcaatctcg aaagcgactt
     4381 gttccgagta agcttttgtt tgaccatagt cgcggccaac aaacacttga tggtttcctt
     4441 catattgaac aggacctaaa cgatcactca ttccatattc tgttaccatg cttcgtgcta
     4501 gagctgtcgc ttgctcaaag tcattggaag caccagttga ttggacattg aagatgattt
     4561 cttcagctgt acgtccacca agtaagccta cgatttgttc aaacatatct tctttcgtca
     4621 tcaagaactg atcttctttt ggtaaagcga tcatatatcc gccggcgcgt ccgcgaggga
     4681 tgatcgttac tttgtggacg acccgagcgc gactcaatac taatccgaca atcgtatgac
     4741 cagcttcgtg ataagcaacc atttcgcgtt cttttttgct gattacgcga tcttttttag
     4801 ctggacctgc aatcacgcga tcttccgctt catcgatatc agaagcatcg attttctttt
     4861 tatttcttct agcagctact aacgctgctt cattcaagac attttctaaa tctgctccgg
     4921 caaaacctgg tgtctgttga gccacaactt tcaagtcaac gtcatctgcc atcggtttat
     4981 tccgtgcatg gactcgcaag attgcttcac gaccttttac atctggacgt ccaactaaaa
     5041 tctggcggtc aaaacggcct ggacgtagta acgctgggtc tagtacatca gaacggttgg
     5101 ttgcagcaat gacgattact ccttcatttc cgtcgaagcc atccatttct acaagcaatt
     5161 ggttcaatgt ttgttcgcgt tcatcatgtc cgccgcccat accggcacca cgttgacgac
     5221 ctaccgcatc aatttcatca atgaaaatga tagctggtgc gtttttcttc gctgtttcaa
     5281 acaaatcacg aacacgactt gctccaacac cgacaaacat ttctacgaaa tcagaacccg
     5341 agatcgagta aaacggtact ccagcttctc cggcaacagc ttttgctaat aaggttttac
     5401 ctgttcctgg aggtccttct aaaagtacac ctgccgggat acgggcacct aattcaacaa
     5461 atcggcgagg atctttcaga aattcaacaa cttcaactag ttcttgtttt tcttcttccg
     5521 cacccgctac atcagaaaaa cgtacgcgat tagctttttt gtctgcttct ttggcttttg
     5581 atttaccaaa gttcatgaca cggccgccac cgccaccgcc gccgccttgt tgtcccatca
     5641 tcatgtaaaa gaaaaagatg atgattacga ttggcaagaa gctaagcaat agggaaaccc
     5701 atataccgct gcttgattct tcttttactg ttgtagcaac tttgttttct tttgccagtt
     5761 ctgttacttg gctcaatgtt gtatcatttg gcaaaatgat tgttgtgaaa tgggtagtag
     5821 tcgtatctgt tgaacctaag atagctaacc caccgctgtt agcgacggtc tgctgttctt
     5881 tgtattcacc agtgatctta tatacaccat ttgcaggctg cacttcaaat tctttgatct
     5941 tgcctgcttg tagttgttct gtgaattttg agtactcgat atctgacgat cgctggccgt
     6001 tattaccaaa aatgaaataa acgaccatta ccatcgctaa gattacgagg acataataca
     6061 aggcattttt catcatgccg ttgttctttt tgttcatagt tgccctccct aagcgtcaat
     6121 ttaatttaac agttttttat caatcctgac cattctattg taccataatt taattgagat
     6181 acagcattaa tttgattgat aaacactagg tttcaataca ccgatgtacg gaaggttacg
     6241 atatgcttct gcataatcca agccataacc gaccacaaat tcatttggta cgttgaagcc
     6301 aacataatct gcttcgatat ttactactct tccttcaggt ttgtcaagta gtgtaacaat
     6361 tttgactgat tttgctttgc ggtatttgaa aagatccact aaataagcaa gtgttcttcc
     6421 gctgtcaatg atatcttcaa cgatcaacag atcgcgacct tctacattcg tatctaagtc
     6481 tttgacgatt tttacttccc ctgacgagac tgttgcattc ccgtagcttg aaacatccat
     6541 gaaatccaat tccaaatacg tatcgataga acgaacgata tctgccatga atggtaccgc
     6601 tccttttaag acacctacta ctaggggatt ggtgttttgg taaatttctg ttaactcttt
     6661 gcctagttct ttgcaacgaa cttgtatctc ttcttgtgag atcaaaattc tctcaatatc
     6721 cttttccaac atcaggttct ccttccaatt gcttcttttt gatatttgta aagaagtatg
     6781 tagtgtattt tatcagtttc ttcacgaata ctcaaatatg aaaaggcaaa aggtatcaag
     6841 ctatatatat ttcttttggc atctgtaatg atccaagcat gctgcctttg tgaattaggt
     6901 actttctgat cgataaaata tctagatact ttttttgtga gattctctgt taatttaatg
     6961 cggtctcctg cttttctttt ttcgacatac agttgcacat cagcaggaat ccaaatgtca
     7021 tgagaaaata gagaccatga atctgttgct ttgatgtccc ctgcattttc aggtttgacc
     7081 agaccaatcc attctgtttc attcaaatat attccttgtt ctggaatcag caacaagcgc
     7141 tggtcttctg tctggctagt tgcttgtttt tcttttttag agagtacaac aaaatgatac
     7201 tctcttttca gtatccaatt attgccaata ctgagttgcc attggctttt tttctgttgc
     7261 cactggtcta aaatcgactc catttgttgt tctttcaaaa taattcctgt ttttgagaat
     7321 gctttgtcga aaaaggagta aaaaaaatag tatcgttcag catctgtcat ttcttcaatc
     7381 atttctgcag aaaatcgaaa ttgttcctcc cactgttcga cttccttttc gatcaactgt
     7441 cccatatatt tttgaatgac ttggtctgcc cattggatct gttgcgaaaa ttgctggaaa
     7501 tgtctcaacg cctgtgtatt ttcttgtttc aattgaggaa ctaccaaatg acgaagccga
     7561 tttctctgga tatctaacaa ttggtttgtc tgatcctcaa agtaaacaag ctgcgattcc
     7621 gctgcataag tatataggtc ttctttgcta aaactcagca acggacgaat cagtctgccc
     7681 gtagcaaatg gttgtacttc tttgattcca gaaaaagtat ttaactgtcc cccacgaacg
     7741 attttcatta acaccgtctc gatttgatca tctccgtgat gagcagtcat caacgtgtca
     7801 tattgtcctt cttccatcac gcgagtgaat tcttgataac gaaagttacg agcttctgtt
     7861 tctatggcgg atttggctgg atcttcccaa atacttacat ataaagggag attcttctct
     7921 tcacaatatg agcgaagata ggcagcttct tctgctgatt cttctcttaa ttggtgattg
     7981 atgtggacga tacctaaaga aaaaccgatt tggcgttgca cttgtaaaag taaatggagc
     8041 aaaaccatcg aatcaacacc acctgatacc gctgctaaaa ttctcgatcc ttgttgccag
     8101 aatccttgtt cattcccgtg ccgaataaat gactgctgta atagcggtgc ttttttcccg
     8161 cctttttcaa cgttcatcct ttccacaccc tttcttccta aaaaaaagag agcgggaaaa
     8221 cgtctaaaac gacaattaac ctgccctctg ttgatactat gacccaaata gtcgcactat
     8281 cattatttgc ggctttttag ctgcgacgtc cgccacggcc accgcgcttt ccttctgtgt
     8341 ttcgcttcaa tgaagataag cggtcatcgc tatctttcaa aaatgaactc atcaatgaat
     8401 cgaagtcttc tttttgagct gaaggttgac tgcgagaaaa agattttttc tgtgctggac
     8461 gatttcggtt gtcacgggga tctttttgtt gatatttttt aggacggctt tcttctccag
     8521 attcagcagg ttgttcctgc gctttccgga tagatagtcc gatttttcca tcatcattta
     8581 ctgaaataac tttaactgtt acttcatctc ctacacttaa aacatcatgg atgtctttta
     8641 catagccatt tgacacttcg ctaatatgaa ctagacccgt ttttccagca cctagatcaa
     8701 taaacgcacc aaaatttgtg attcctgata cttttccttg cagttttgct cctacctcga
     8761 ttgacataaa aaaaatgttc ctcctaatta tttccctttt ttatattcct gcttattttg
     8821 cagttgtaga actagttgat tgatctgttt catttgtcga gctgtctgta tttgattcta
     8881 atataggata aactttttca ccgtcttttg agtaaaagaa tttgcttctg gcaagtttag
     8941 ctacatactc atcatctttc agcaatgaaa catcttggtg cagctgatca acgtctgcgt
     9001 tgacttcttt catttcagta tttgcttcga ctctcaattc gttcaattga tgcatccttt
     9061 gcatgtcatg gaaaatctgc acacctatta tcgcgaaaat gaccaaagca ccaacaaaaa
     9121 ttgcagccag tctgcgtctt cgaaaaatca gttgacgttg ttgttgctga aattcagcat
     9181 actttttctt tgcatattcc gtatctagcg cagcgatttt attgcttttt ttcgtcactt
     9241 ggattcgctc caatcttttt gattgtcttt ttcattatac caatatcgtg aaagcttgtc
     9301 taatctattt ctctagtgtt tctgtgcgat tttcagaaat aatttcgtac attttttgtg
     9361 catcttcttt tttggttgat tcatgtaatt ccgtaatttt cacttccaac gttttgttgc
     9421 caaactggac tttcaaaata tcgcctactt tgacatcact ggaagatttc gccaatttac
     9481 cgttgatttg aattcttcct ttatcagcta cttcttttgc tactgatcgt cttttgatga
     9541 ttcgggaaat ttttaaaaat ttatctaatc gcatatctta ttcctcactt tttaattttt
     9601 ttatttaatc gcaggatttt cttaccaaaa ggcaacatta gccattctcg tactgtaaac
     9661 agacgaagac gaatcactgc ataaatgaac acagaagccc cgatcccaac tcctatcacg
     9721 ctgacaaaaa aagcagtgga acgatgagtt acaggaccaa atactatatt caatacacca
     9781 taatacagca gcagtactac tgtcatacaa agaaggctga acatcaactt ccgtccaaaa
     9841 tgccgttctt tccaaaaagc atttagctct tttgtttcaa ttcgaacaaa ataccaaagc
     9901 gtcacaccta atcctaaaag ggtagacagg cttgcacctg ctgttcctaa taaaccggtc
     9961 agaaaagaag tagtcaatcc ttttaccaat agtccccatc ctgcgccttt taaagaaggg
    10021 cgaaacgagt tcttgctttg agcaatactt tgataagctt ggatgactgc agtgaaagca
    10081 atggaaaaaa caaataatac aagcgctgca tttcccgcat aatctttaaa caacgcataa
    10141 ttgatataag ggagcagcaa ggctaatccc aaagacgcag ctaatgctaa agcggtcgtc
    10201 aatctaagat agattttggc tgtctgtaaa aactgacgat agatccggtt ggtcaaatga
    10261 cgagtcaacg caggcaggaa tgtcgcactt aatgcagtgg caatcactaa acctaattgt
    10321 actaaaggct gacctcgatc atagacccct tttgctatct tcgcaccgta ttctgacagt
    10381 ccgtaaactt ccaaagcatt ttttacaaag aaagagtcga tcagctggaa aaagataagc
    10441 aatccactat aaatcgaaac cagtccgcct tctaccagaa aacgccggat caagggcttt
    10501 gacggctgtt tgaacaatgg aaaccgtcgg aagcttaacg ctcctccatg aatcttttgt
    10561 tcatagtagt gaaggatccc atatgcgcat aaaccgccaa aaacagctcc actcattgct
    10621 gccgtccctg tttggtacac cgtccatcca agctttctaa aagcaaacgc actacagaca
    10681 atcacaaaaa cacgaacgaa ctgctcaact acttgagaaa cagcagttgg ttccatcaag
    10741 aatcttcctt ggaaattccc tcgataaaaa gatagaccag gcatcagcaa aaaggtaaag
    10801 gaaactactt ggatcagcgg tttcaattgt atgtctccca tcatccaagc aatccagctg
    10861 ctaaatgcaa agacaaacaa ccacaaaaag acacctgtcc aaaatactaa gggataaaga
    10921 ttttgcaaag cttgctttcg ctcgaaagga ttctttcttt ctgcaacgta ctttgatatg
    10981 aactgcggaa gaccggataa agctaaagtc attgctatcc cataaattgg ataaacttgc
    11041 tgataaacat aaaaaccttc gtcgccgact aagttttgga ggggaattcg gtaaagggca
    11101 cttaatactt ttgctatgaa agaagcaatc gtcaatacaa atgctccttg catgacgcgc
    11161 cgcatttctt tatgatccaa atcattgctc cttcctacgc tgacagatat ttttgctcac
    11221 gcaacgcttt gacaaacttt tggatttcct gaagccatac tgcttcttcc atttggttgg
    11281 ggatcgtcaa gcgtatcacc atctgttctt tttcaacgcc tagactggct tttagttttg
    11341 tcgctgtcag tgcttcgaat aactgttcca cactatagcg ttttgttcca actttactta
    11401 gagtaaagac tatatgcgcc tcctgtttcc ttatcgtttc taggagcgct cgatcgccat
    11461 ccattttgat ctgcccaatt gtcaagagat gtgctacttc ttctggatat tctccaaaac
    11521 ggtcgagcag atcatcttct agttcatcta gcatttcttg cgaatcaagt tcacgaattc
    11581 gtttatagat ctcgattttc tgccgttgat ctgccacgta tgtttcaggt agatacgcat
    11641 cgacaccgag gtcgatctca acagtggtct tctccacttg actattcttt ccttgtttac
    11701 gagaaacagc ttcgcttagc atttgagaat acatatcgaa accgactgca tcaataaatc
    11761 catgctgctg agcacctaag agattacctg caccgcgaat ggaaagatcc cgcatcgcaa
    11821 tcttgaatcc agatcccagt tctgtaaaat ctttgattgc ctgaagacgt ttttcactga
    11881 cttcgttaag gatcttctgc tgctcataca taaagtaagc atatgcgaca cggctgcttc
    11941 ttcctacccg ccctcgcaat tggtataacg tggataatcc catataatca gcgttctcaa
    12001 cgaacaacgt atttgcgttc gggatatcta ctcctgtttc aatgatggtt gtagtgacaa
    12061 gaacatcata ttgaccttca ataaattcaa aaagcgtatt ttctaattgc gcctctgtca
    12121 tttgcccatg agcataaccg atccgtgctt ccggaacgag ttcttggatt tcttctactt
    12181 tttgttcgat cgtttctacc cgattatata aatagaatac ttgtccacct cgtcccattt
    12241 ctcgatggat tgcttcgcga atagccccag gatttttttc cataacatac gtttggatcg
    12301 gataacgatt cgcaggcggt gtttcgatta ctgaaagatc ccgcacgcct aacatcgaca
    12361 tatgcaaagt tctcggaatc ggtgttgcag tcagagtcaa aacatccact tgtgcacgta
    12421 actgtttcaa tcgttcctta tgttttacac caaaacgttg ttcttcgtca atgatcagta
    12481 gacccaaatc gctaaattca atatcttttg acaaaattcg gtgggttcct acaacaatat
    12541 caacttgacc tgtacgcagt tgttcgatgg tttctttttg ttgctttctt gttctgaaac
    12601 gacttaatag cccgatattt acggggaaac cttcaaatct ttccagcatc gtttcatagt
    12661 gctgctgtgc caagatcgtt gttggaacta aaaaggctac ttgtttgctt tcctttatcg
    12721 ctttaaaggc cgctcgaagc gctacttctg ttttcccata accaacatca cctacgagta
    12781 gtcgatccat tggtttttct ttttccatat cccgtttgat ctccgctgca cttctcaact
    12841 ggtcatcagt ttcagagtag gggaaggcat tttcgaattc tttttggtag gcgtcatcag
    12901 gtccaaaagc atatcctttt tctgactctc ttgcggcata taatttgatt agatcgtcag
    12961 cgatatcctc gatttttgaa gagacttttc gcttggtttt cgtccattca cttccgccta
    13021 gcttattgat gcgaggcgct ttggcttcag aggcgacata tttttggatc agattcagtt
    13081 gagtaacagg aataaacagt ttgtcgtcgt tttgatacaa aatagtcata tagtcttggt
    13141 gaacgccatc tacttctagc gtttccatcc caatgtattt cccgatacca tgattggcat
    13201 ggacaacgta atctcctgct tttaactcat tatagctttt caatcgctct gcgtttgtta
    13261 ctgtttgacg tctcgtttgt tttttcgtcg ttttttggaa aatttctttt tctgtgatag
    13321 ctactatttt ttcttgagga agttcaaaac cagcctgcaa agctccttca actaattgta
    13381 cctgtcctct gatcaatgca tttttttctg tttctactac taatatgtct tcatctcgaa
    13441 gcatttgttc tgctttgcgt attctgtcct ttgtcggaat aaagatcaca acagtctgtt
    13501 tttgcttgcg ccaacggtcc atttctgttt tcagcaatgg catttgtccg aagaactgtt
    13561 gcatcgtccg atattgaaaa ttatggattg cttggaaacg aagatttccc atcccttttt
    13621 ggaagagtga gaaaaaggtt gtggtgaact tttccttctg cacgattttt tgtatatctg
    13681 ctgaaaatgt ctgctcagaa aacacccgca attcgctgat tttttgagtc tgccattctg
    13741 ccgcttccct ttcgatttcc cgatttgttt ccatgattcg ctgataatca tcaacgacta
    13801 gtaaactatt tgaagggaaa taatccaaga tcgtcacaga ttcttggtaa agaagatctg
    13861 agtagaattt tgcttcttct gtcgggacac ctgcttgcca gcttgaagtc agctgaccga
    13921 agtaatcttg caaaaactcg cggtctgctt cttctgtggt cgcagcaagc cgtttttcta
    13981 gcattttttg catttgctct attccatgtt ctagatcctc tggtccgtag acttgttcac
    14041 tcgttgggga aacccagatt tcttccgttt tttcgattga gcgttgtgtt tctgcttcaa
    14101 aataccgcat agaatctatc tctacatcaa atagttctac acgaacagga taggttgtcg
    14161 ttaacgggta aatatcgata atgcttcctc gaatactgaa ttctcccggt tttgcgacca
    14221 tctcttctcg tacatagccc atcaaagtca attggcgagg aagattttcc acgtctatct
    14281 catcaccaat atgccaattc aattgcgcct gtagccaagt tttcttattc ggcagtcgtt
    14341 tgtgcaaaga tgcgactggc aacaaataga tacctgcttt tttttcttga agtgcgttaa
    14401 gcgttgcaac tctctcgcct cgcgcttctg gagaagaaaa ggccatttca gcagaaagca
    14461 cttcatccac tggaaataaa tggatctgat cttgcggaat cacatgttgc agatcttctg
    14521 ctaactgatt cgcataatac aaatttggca tcagaataat catgttttta tttgttttct
    14581 taaacgctcg agcaaataga agtgttttcg ctgagcccgc taatcctgtg attagttgtc
    14641 taggcatttt ttcttctaaa ttgctgaccc attcttttac ttgcggatct tttccgacta
    14701 attgaataat atccattcct tttcctcctt cctcaattga atcgattcat cgcatccacg
    14761 aacgaatgcc cttctatgag atattcgact gctttaacag attgacacat gctttcttct
    14821 atcaaaggct gattttcttt gctgaaagga ctaagcacat gctggactac tgtttttttc
    14881 ccttctggcc tgccgattcc caccttgatt cgatcaaaga cattcgtatt caaatgggaa
    14941 ataatacttt tgataccgtt atgcccgccg gcacttccct tttgacggag ccggattttt
    15001 cccactgcca gatccaaatc atcgtagatt acgactaatt cctctggata aatcccaaaa
    15061 taagtcatta gtgggccgac tgcacgacca gattcattca tgaatgtttg tggtttgact
    15121 aaaaggatct tttctccatt atgaaaaaat tcggcaactt ctgcttcgaa cggatttttt
    15181 ttgaaagtag cttgatgctc gcgtgcaatt ttatccacga ccataaagcc gatattgtgt
    15241 tttgtatatt ggtatttggt acctgggttt cctaacccga cgatcatttt catttttgtt
    15301 ttccacctaa ttctaaaaag cagcacaaaa ggctggactg ctaattagcg gtccagtcca
    15361 tcgagctttt ttctttttta cacttgtcaa tgcatagtat aacacaaaac aaggcgtttc
    15421 ggctaaaaga gaatcgttaa taaatgataa agaaattaaa aatttcttca aagtttaatg
    15481 aaataattga tatataaaaa tagtgaaata acgcacatgt aagcttttac atataataca
    15541 accttgttat ctgttatatt ttatagccaa atagcgtgac atggggcttg agaaatgtta
    15601 gcataagcat gggaaaaaac aaatcaatgg aaggattgat actaaatgac tgcagctgca
    15661 gaaaaaaaag atcatcaaaa agttattctt gttggagacg gcgccgtagg ttctagctat
    15721 gcctttgctc tagttactca aaatatcgct caagaagtag gtattgtgga tatcaatact
    15781 gctaaaactg aaggagacgc aattgacttg tctcacgctt tggcatttac ttcacctaaa
    15841 aaaatctatt ctgctactta cgcagacgca catgatgctg acttagttgt tattacagct
    15901 ggcgcaccac aaaaaccagg tgaaactcgt ttagacttag taaacaaaaa cctaaaaatc
    15961 aaccgcgatg ttgtcacaca aatcgttgaa tcaggattta acggtatctt tttagttgcc
    16021 gctaacccag tagatatttt gacttactca acttggaaat tctcaggttt ccctaaagaa
    16081 cgcgtaatcg gttcaggtac ctctcttgac tcagctcgtt tccgtcaagc acttgcggaa
    16141 ttagttgacg ttgatgcacg taacgtccat gcttacatct taggtgaaca cggagattca
    16201 gaattcccag tttggtcaca tgcaaatgtt gccggcttgc aaatctatga atgggtaaaa
    16261 aacaacccag aaatcgatga agaagctatg gttaacctat tctttggcgt acgtgacgct
    16321 gcttacacaa ttatcgagaa aaaaggcgct actttctatg gtatcgcagt agcattagca
    16381 cgtatcactc gtgccatctt agatgatgaa aatgctgttc ttcctttatc agtattcatg
    16441 aatggcgaat atggattgaa cgacatctac attggtgcac ctgctgttat caaccgtcaa
    16501 ggtatccaaa aagtaatcga aattccttta agtgacagcg agcaagaccg tatggctgct
    16561 tctgctaagc aattaaaaga aattctagat gaagcttttg ctaaactaga cgctgaataa
    16621 tctttgaaca tgacggaaaa catctattcg gtgttttccg tttttttatt ttatcaccct
    16681 tatggtttct aaattttttc ataaagtaga gataaccttc gtcttttcct ctaaattatg
    16741 ctatcatgag accgttccac cctaaacttt tacttaagga gttgtcgata tgacaagatc
    16801 ttcgcatcga aaaaaatcga aacatgttgc tttgctttca gttcttggtg ttctcttagt
    16861 gctgatcagc ttttatacct atcgcagtac atactacaca aagcattttt tgccgaatac
    16921 agaaataaat ggaataaatg tcagcaatct aacagtcgaa aaagcaaatg aaaaattaaa
    16981 agaagcttat tctgataaaa aactggctat taaagaagat ggaaaagtct ggcaggaagt
    17041 accaaaatcc gagctcggct acaaagatga tttcacttcg gagctttctc cgatcctaaa
    17101 tgaacaaaac ggatggactt ggggaatgac ttatgtctca gcggcagaaa aacaagaaat
    17161 agatcctgtc agtcaaaacc accaaaaact agacactacc gttcagacat tgacaactaa
    17221 gctgactgat ttgaacaaag accggacacc tactactgat gcaacgatcg aaaaagcagg
    17281 tgactctttc gttatcaagc ctgaagtcaa tggaaatacg atcgacgtcg atgctgcggt
    17341 caaacaatta aaaagtgcgg taaactctgg taaagataca attgaattga ctgaattcaa
    17401 agaaaaacca aaagtaacat ctgaagacag ctcactgaaa gaacaactcg catccatgaa
    17461 tgctatcgca aatgtaaaag ctacttacag tataaacggt gagactttcc agatcccatc
    17521 aagtgatatt atgagttggc tgacctacaa tgatggaaaa gtcgatttag atacagaaca
    17581 agtacgtcaa tatgtaacag atttaggcac aaagtataac acaagtacga atgacacaaa
    17641 atttaaaagt acaaaaagag gagaagttac ggtcccagtt ggcacttaca gttggacgat
    17701 ccaaacagac agtgagacag aagcattgaa aaaagcgatt ctggcaggcc aagattttac
    17761 tcgttcaccc attgtgcaag gtggcacaac cgctgatcat ccattgatcg aagatacgta
    17821 tatcgaagtt gatctggaga accagcatat gtggtattac aaagacggaa aagtggccct
    17881 tgaaacagat atcgtttccg gaaagccaac tacacctaca cctgctggag tcttctatgt
    17941 ttggaataaa gaagaagatg caactttaaa aggaacaaat gacgatggaa caccttacga
    18001 gagtccggta aactactgga tgccgattga ctggacaggt gttggtatcc acgattcaga
    18061 ctggcaacct gaatacggtg gagacttatg gaaaacccgc ggttcacacg gctgcatcaa
    18121 caccccacca agtgtaatga aggaattatt cgggatggtc gaaaaaggaa cgcctgtatt
    18181 ggtcttctaa caaaaataat atataaaaag aaagcacccg tacgattggt acaggtgctt
    18241 tctttttatt atctaacaat gtgctactta catttcagca gcagtaaaga cttctgaaac
    18301 atcatcatcg tcttctagct tatctactag acgttccaat tgctcttttt gttcctcatt
    18361 taaatcaatc gtcgtttgtg gaatcatcgt caattctgct tgcgccaaag tgaactcttt
    18421 ttccaactca tctcgtacag cagcaaaatc ttctggtgca gtataaatct cgaatacttc
    18481 aggagaagtt tgcagatctt ctgcccccgc ctctaataca tcctcaaaca tcgtctcttc
    18541 atcaacagtc aacccatcgc gttcaatagc gatatatcct ttgcggtcaa acaagtaact
    18601 gactgaaccc gtttctccca gtgatccacc gtttcgagta aaagctactc ggacatttgt
    18661 cgctgtccga ttacggttat ctgtcaatgc atggactaag attcctactc cacccggacc
    18721 atatccttca tacgtgattt catcgtaatt cgtttcatct acggcactcg tagccttttt
    18781 aatcgcccgt tccacattgt cgttaggcat atttgcagct tttgctttgt ccacaacaag
    18841 ccgcaaagct ggattcatag atggatcagg accgcctgct ttagctgcca tataaatttc
    18901 acgagaaagt ttctgaaaga ctttccctcg ttttgcatct tgggcatttt ttcttccttg
    18961 gatattatgc catttactat gtcctgacat gatttttcct cccttgttct tttgagaata
    19021 cacttctatt ctagtactat ttcacaaaaa aacaaagcca cagccaatct ataaacgctt
    19081 cttgaatgcc gcaaaaagca cacataatat cacagcagtc agagcaccga tcgaatccaa
    19141 catgacatct tggaacatag gtgttcgtcc accggtcatc atctgatgga attcgtccaa
    19201 acctgcgtac cctgttgctg aaagccaagc aaggatcgct gtcagccacc aaatttttag
    19261 cttggggaaa acacctaaaa acaagctgcc tcccaaaata aaataggtac cgaaatgtgc
    19321 gcccttcctt aaaaagaact ctaaaaattt ggcgtagccc agatggttga tactcaccat
    19381 acttccacca taattaaaac gtatacccgc taatgtgtct ttaaatggct gattaggcag
    19441 ccagctttct aatcgcgaaa cttgtgattg ctgtccgtaa gtttgagaag aactgtaaaa
    19501 taagattata acgactatca gtgcgattac taaataaata tttccgtttt tcatttgttt
    19561 ttttgaagaa ttcataagtc acctctgtat tttatcttag actttttttg aagaaacaac
    19621 aatcattttc atagagactt cttccataaa aaagtgctat acttaatttt gtattacaat
    19681 atgaagggag ttttttcatg agttattcat taaattggaa tctagattct attttttccg
    19741 gaggcagtca ttctgatgct ttgaaccaac ggatgaagca attggaagac caaatgaacg
    19801 agtattatca ccgcgtaaca aaatggtcac ctagttctga caatgcagag cagttaaacg
    19861 ctattcttca attgcaagaa acgattacga atggttttac ccaatgcagc agctatatta
    19921 ctgcattgct ttctgccaat gtcaatgatt cagatgcaaa ggtcttatca ggcaagcttt
    19981 acgcaatgct gcctcgtctg caatcagccg aaactgtatt gtcaaagaaa tttgcagaaa
    20041 tatctgacaa tgattggaat cagatgcttt ctcaatcttc gtttgaaaca attgcttttc
    20101 gtttaaacga gattcgtcgt gatggctcac aattattatc agaagctgaa gaaaacatta
    20161 taaatacgct ttctttagat ggtctaaatg cttggagcag tcattatgat acgattgtag
    20221 ccagcatttc gattcccttc gaacaagatg gacaagtagt cgaattatct gctggacaag
    20281 ccttcaataa gatgatgggc gatccagatc caaaagtcag agaaacactt ttcgcagcat
    20341 gggaagaagc ctggaaagaa aaggcacccc tcttcacgga tactttgaat catttagatg
    20401 gttttagact ttctgactat gaacttcatg gtgtcacaga ttttctacaa aaaccattag
    20461 agtacaatcg gttgaagaaa gaaacattgt ctgtcatgtg ggatacaatc cagaaaaaca
    20521 aacaacccct tgttaattac ttgactagaa aagctaatct tttcggtaaa gaaaaaatgg
    20581 aatggcaaga tcaagatgca cctattattt taggcgattt gaaagaaaaa acctttagtt
    20641 ttgacgaagc agcagcattt attatcgaaa actttaacaa attcagtcca aaaatggcta
    20701 aatttgccca atctgctttt gaaaagagct ggatcgaagc agaagatcgt cctgggaaac
    20761 gtcctggtgg ttattgtact gagttaccag aaacaaaaga atcaaggatc tttatgacct
    20821 acagtaattc tgttaatgag gttgcgacac ttgctcatga actaggacat gctttccata
    20881 gcagcacgat gtgggattta ccttctttaa atcgcgaata tgccatgaat gtcgcagaaa
    20941 cagcaagtac attcgcagaa ttgatcgtag ccgatgctac attaaaagtc gcaaaaacaa
    21001 aagaagaaaa aatcaatttg ctagatacaa aactgcaaaa tgctttagct atgttcatga
    21061 atatccattc ccgattcatt tttgaaaatc gattctatga agcacggcaa gaaggattag
    21121 tctcagaaga taagatcaca gagatgatgg ttgaggcgca aaaagaaggc tatcaaggag
    21181 ctttagctac ttaccatcca tatttctggg ctgcaaaatt gcatttcttt attgatgacg
    21241 taccgtttta taacttccca tacacatttg gctacctctt tagtttgggc atctatgctt
    21301 acgccaacca aaagggagca aattttgaag aagaatatat ccatttacta cgcgatactg
    21361 cctcaatgac gacggaagat cttgcgaaga aacatttgaa tgtcgatctt acccaaccag
    21421 atttttggca agcaggtatc gacatggtga ctgaagatat ccaaacgttc atggaattga
    21481 cagaagaatt tatctgattt ctattttatc acaataaaaa aagagttagt ccagaatggg
    21541 ctaactcttt ttgatgtgcg ccctgcatgg gtgaaaactt ggtggtgaaa gtccactaca
    21601 ggcttggcag taggaactgt tagcgaaaga caaggcggca atcgtgaggt tgtggctgaa
    21661 ggaagtcgga cgcaaactcc tgaactgacg aacagaaact agatacaagg ctgaattagg
    21721 tcggataagt cttcatcaca agacaaagtc caatactgcc cgaaacctat acagtaaatc
    21781 tagcagttac atgggaggaa agttttcact cttaccgggg gagatctcat gagggtgttg
    21841 tgggcgttga atgaaaacaa accagcacaa cgatcatggc gacatgatgg tgaatcatga
    21901 gaagtcagcc gaggtcatag tagtaacgac actgaccgtt acgaaggact gaacaataag
    21961 aatcttgaat aatttaggag gtgtgagaag atgcgacgac cgcagaaaac aagaaaactt
    22021 ggctatcatc aaagagctag tgtggaacac gaagggtatg atgaagtgcg cagcacttct
    22081 tgcggtgaac agaggaaaca agatggtgta gaaaatctgt ttgggagaat tctagatcgg
    22141 aataatctca atcaagcgta tcttcaagta gtcagaaata aaggggcggc tggtgcagat
    22201 gggatgaccg tcgatcaact gcttccttac ttgaaagagc accgcgagga attactatcc
    22261 aatttacgcc aagggaacta taaacctcag cctgttttgc gagtagaaat accgaaacca
    22321 aacggcggtg taagaaaact aggaattccc acggttattg atcgtatgct tcaacaggca
    22381 atcaatcaag tgttacaacc gctatttgaa ccagaattct cagataatag ttatgggttt
    22441 cgcccaaatc gcagcgcaca tgacgcagtg aagaaagcaa aatcttatta tgaacaaggt
    22501 taccgatatg tggtggacat tgatatgaaa gcctactttg acaccgtaaa tcacgataag
    22561 ctgatgtatt tcattgagcg gaaagtaaag gacaaacaag tcctaaaact gatccgtcaa
    22621 tacttaatga gtggtgtaat ggaaaatgga ctcatcacaa caactgaaga aggaacgccc
    22681 caaggaggca atctctctcc cctgttaagt aacatctacc tgaacgaatt tgatcaagtg
    22741 ctagaaaatc taggacataa atttgttcga tacgccgatg actgcaatat ctatgttaga
    22801 agcaaacgag caggacagcg tgtgatgaag aaatccattc aatttctcga agaacaacta
    22861 aagttgacgg taaatcgaga aaagagtgcg gtaggaagtc cattgaaacg aaagtttctt
    22921 ggattctgca ttctgccaac aaagaaaggt attaaaattc gccctcacgc aaaagcaaaa
    22981 cagcgtgtga aaagcaagct caaacaactc actaaaagaa accgtggaag aagtatccaa
    23041 cttattttaa aagaaatcaa gcaactaatg actggttgga tcaactacta tggaattggt
    23101 gagatgaaag aattcatgag ggagttaaac ggttggttga agcgaagaat cagacaatat
    23161 atttggaaac aatggaagaa tccaagaacc aaaagaaaaa atctgattcg tttagggatc
    23221 gataaagcga aagcgtatga atggtcaaat acaagaaaag gtatttggtc agtctccaaa
    23281 agtcatattc ttcatcgatc attaacagac aaagaactgg cccgccaagg atatgaggac
    23341 atctccttga agtaccagtt cgtacactca acttattgaa ccgccgtata cgggtccgta
    23401 cgtacggtgg tgtgagaggg gcggaaggtc agtt
//
LOCUS       DAZBTF010000042        23335 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000042 DAZBTF010000000
VERSION     DAZBTF010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.042"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(193..900)
                     /gene="deoD"
                     /locus_tag="ACHGNS_001710"
     CDS             complement(193..900)
                     /gene="deoD"
                     /locus_tag="ACHGNS_001710"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFN6937653.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(917..1735)
                     /locus_tag="ACHGNS_001711"
     CDS             complement(917..1735)
                     /locus_tag="ACHGNS_001711"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFN6937654.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            complement(1759..2925)
                     /gene="deoB"
                     /locus_tag="ACHGNS_001712"
     CDS             complement(1759..2925)
                     /gene="deoB"
                     /locus_tag="ACHGNS_001712"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFN6937655.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            complement(3024..3980)
                     /locus_tag="ACHGNS_001713"
     CDS             complement(3024..3980)
                     /locus_tag="ACHGNS_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6937656.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            complement(3980..5113)
                     /locus_tag="ACHGNS_001714"
     CDS             complement(3980..5113)
                     /locus_tag="ACHGNS_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6937657.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            complement(5106..6668)
                     /locus_tag="ACHGNS_001715"
     CDS             complement(5106..6668)
                     /locus_tag="ACHGNS_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6937658.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            complement(6880..7962)
                     /locus_tag="ACHGNS_001716"
     CDS             complement(6880..7962)
                     /locus_tag="ACHGNS_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN6937659.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            complement(8028..8426)
                     /locus_tag="ACHGNS_001717"
     CDS             complement(8028..8426)
                     /locus_tag="ACHGNS_001717"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFN6937660.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            complement(8447..9109)
                     /gene="deoC"
                     /locus_tag="ACHGNS_001718"
     CDS             complement(8447..9109)
                     /gene="deoC"
                     /locus_tag="ACHGNS_001718"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFN6937661.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            complement(9155..10456)
                     /locus_tag="ACHGNS_001719"
     CDS             complement(9155..10456)
                     /locus_tag="ACHGNS_001719"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFN6937662.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            complement(10597..11613)
                     /locus_tag="ACHGNS_001720"
     CDS             complement(10597..11613)
                     /locus_tag="ACHGNS_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN6937663.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            complement(11921..12526)
                     /locus_tag="ACHGNS_001721"
     CDS             complement(11921..12526)
                     /locus_tag="ACHGNS_001721"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN6937664.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            12722..13645
                     /gene="coaA"
                     /locus_tag="ACHGNS_001722"
     CDS             12722..13645
                     /gene="coaA"
                     /locus_tag="ACHGNS_001722"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFN6937665.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            13704..15269
                     /gene="guaA"
                     /locus_tag="ACHGNS_001723"
     CDS             13704..15269
                     /gene="guaA"
                     /locus_tag="ACHGNS_001723"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFN6937666.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(15760..17391)
                     /locus_tag="ACHGNS_001724"
     CDS             complement(15760..17391)
                     /locus_tag="ACHGNS_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFN6937667.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(17405..19078)
                     /locus_tag="ACHGNS_001725"
     CDS             complement(17405..19078)
                     /locus_tag="ACHGNS_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFN6937668.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(19091..19408)
                     /locus_tag="ACHGNS_001726"
     CDS             complement(19091..19408)
                     /locus_tag="ACHGNS_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFN6937669.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(19433..20833)
                     /gene="lacG"
                     /locus_tag="ACHGNS_001727"
     CDS             complement(19433..20833)
                     /gene="lacG"
                     /locus_tag="ACHGNS_001727"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFN6937670.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            21070..21855
                     /locus_tag="ACHGNS_001728"
     CDS             21070..21855
                     /locus_tag="ACHGNS_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFN6937671.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            21858..22550
                     /locus_tag="ACHGNS_001729"
     CDS             21858..22550
                     /locus_tag="ACHGNS_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN6937672.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(22824..23195)
                     /locus_tag="ACHGNS_001730"
     CDS             complement(22824..23195)
                     /locus_tag="ACHGNS_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFN6937673.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT         6710 a         5222 c         4217 g         7186 t
ORIGIN      
        1 aaacatgaaa agctgttttt tgaaattgtt ccttgtgact cagagttaaa cggcagattc
       61 acaacctctt ggtaaacggt gttcaaaagc ttacatttgc aataataagt ccagccaagt
      121 caaaaacatg aggagctatt tttgcttgga tgttcttatt acttaatgca gggcagcttt
      181 ttcatttcct tcttattctg ctatcgctgt ttccaatcct acttcgatca tttcattgaa
      241 cgtagattga cgttctgcag ctgttgtttc ttcaccggtg atcaaatggt cgctaacggt
      301 catcaaactt aatgtctgca catggtattt cgcgcctaag taataaagag ccgctgcttc
      361 catttccact cctaaaacgc caagttcagc taattggaac gtctctgtca aatcatcttt
      421 gtagaaagaa tcttccgaaa gaacatttcc tacatgaacc gtatagccct tttctttagc
      481 tatctcatat gctttcagta atagattgaa atcactgatt ggaggaaaat gaaaggcttg
      541 gaaatttttt tcgatcatcg aagaactggt cgctgcacct tgtgctagta ctaaatcacg
      601 aacatggacg tcttttgaca atgcaccgca tgtacctact cgaatcaatt tctttacacc
      661 atacgactgg atcaattcat gggcatagat ggtggctgat ggcattccca ttcctgttcc
      721 ttgaacagaa atacgttctc ctttatattt gccggtaaag cctaacatac cgcgtacctg
      781 attgtaacaa actggatctt ccaaaaaagt ctccgcaata tatttagctc gcaaaggatc
      841 tcccggaagt aaaatcttat cggcgatctc gccttctttc gcttcaatat gcacactcat
      901 tttattgttc ctcctattac aataatgtca atgtttcttt aaccaatgct ttaaaagatt
      961 gtttgactct ctgagttgtt tctacaactt cttcatggct cagattcgct tgcatacctg
     1021 ctgctaagtt ggtaatacaa gagattccta acacttttaa tccgctgtga gctgctacga
     1081 tgacttctgg aacagtcgac atccctactg catcggctcc tattgtccga gacattcgga
     1141 tctccgcagg cgtttcataa gttggtccag aatagcccat atatacgcct tctttaagat
     1201 ccaaacctag tttttctgct gctgcttttg ctgttgcttg atagtcaggc gtataagctt
     1261 gactcatatc tggaaatcta gggcccattg catcttcatt cggaccgatc aaaggatttt
     1321 caccagtaaa attgatatga tcggtaatca gcatcaaatc tcctggtaca aatgattcgt
     1381 tgacaccacc acatgcatta gtcacaatga ctgaatgggc ccctagtccg gccatcacac
     1441 gtactggata ggtcactgtt tgcatggaat gaccttcata gtagtggaat ctcccttgca
     1501 tggctaacac tttcttgcct gccagagacc cataaactag ctttcctgca tgcccaacta
     1561 ctgtcgaaac aggaaagaac gggatctcat cataagaaat cattacagca tcttcgattt
     1621 catctgctaa ttcacctaat cctgaaccta gaatcaaacc aaaatcaatt tcttttactc
     1681 ctttatcaag gataaactgg gtcgtttctt gtaacatctc gcttaacttg gtcatttgct
     1741 ttctcctatt taaaaagatt attttaataa tttcaagaag ctttcgccat tttcagtcga
     1801 agaaacacta aagttctcag caatcgttgc tgaaatatct gaataatatc cttgaggcaa
     1861 tgccccttgt tctttcattt ttttactgta aaccaataac ggtacatatt ctcgcgtatg
     1921 atcggtacca gtaaacgtcg gatcgtttcc gtgatcggct gtaatcatta gcaagtcgtc
     1981 ttcttccatc gcttcaagca attctggaat tcgtaagtca aattcttcaa tcgcatgcgc
     2041 atagccgact acatcccgac gatgaccgta taatgcatca aaatctacta aattggtaaa
     2101 gctgattcct gaaaaatcgt ttttcataac tttcagcagc tggtcaacgc catccatatt
     2161 gcttttcgtg cggacagatt cagtgattcc ttgtccgttg aaaatatcgt tgatctttcc
     2221 aacagcaatc acgtcctttc ctgcttcttt caaagaatca aggactgtgt gtccgaatgg
     2281 attcaacgca taatcatggc gattgcttgt tcgtgtgaag tctcctggtt cacccacgta
     2341 cggacgtgca atgattcggc caatcatata cggatcatct tttgttatat cacgaacgaa
     2401 ttggcaaatc cgatataatt cttctaaagg aatgatttct tcatgtgccg cgatctgaag
     2461 gacaggatcg gcagaagtat atacaattaa atcgcctgtt ttcatttggt gttcaccgta
     2521 atcgtcaata acagctgttc cgctataagg tttgttacat acgactttac ggccagaaaa
     2581 ttcttcgatt tgtttgatca gatcatcagg gaatccatta ggaaacacac gaaatggagt
     2641 tttgatattc aatcccatga tttcccagtg gcctgtcatc gtatctttcc ctacagaaac
     2701 ttcttctaat ttcgtcgcat agccttgatg atctttcact gattccacac cttcaagagg
     2761 acggatcgtt cctaaaccaa gctgctctaa atgtggaacg ttcaatcctg cttctttcgc
     2821 aatgtgtcct aatgtatcag aaccaacatc accgaactta tcggcatctg gtgcttctcc
     2881 aattcctact gaatccatga cgatcagatg tacgcgttta aacatgatta ttccttcttt
     2941 ctttttattt ttattgcctt taaaacaaaa agggcgagac gaaaaacact tcccgtaacc
     3001 cacccttttt attcgctaaa agattatttt gatttgatat agttcttccc gtttgctttt
     3061 ggaccagaag ctttccctaa gaaaacaacc agcacaatga ttgtcagcaa atatggagca
     3121 atttgcagca atactggcgg aatcgaagaa ataactggta attgtgcgcc aatgatactc
     3181 aagctttgag caaaaccaaa gaacaatgag gcacccattg cccctaaagg attccactta
     3241 ccaaagatca tagcagccat ggagatgaac ccttgtccaa cgatcgtacc agctgaaaag
     3301 ttaccggaaa tcgactgagc aaaaacagca ccgcccatac cgcctaacaa gccagaaatc
     3361 agtacgcctg aataacgcat agcatataca ttgataccta atgtgtcggc agcttgagga
     3421 ttttcaccaa ctgaacggag gcgcaagccg aatttcgtct tgaagatgat cacccaagct
     3481 aggacagcaa tcaaaatagc acaataagca ggtaagaatg tatttttaaa gaaaattggt
     3541 ccaatgaccg gaatatctgc taatcctggg aaagaaaaat accccagatt ctgcgtgatg
     3601 ttatcagttt gacctttacc ataccatgct ttgatcaaga aaacacctaa agcaggtgcc
     3661 atcagattaa ttactgtccc gctgataata tggtcagcac gcaaactgac tgttgctact
     3721 gcgtgaatca gagaaaatag gatacctacg acgccaccca ctaaaatagc gatccatggt
     3781 gtcatagaac caagtgatgg actcatcgtc aagttgaaaa cgatggaact aaaagcaccc
     3841 atgaccatga ttccctctag ccctacgttt acgatcccgc ttcgttcaga aaaagttccg
     3901 cctagtgacg ttagaattag cggtgttgaa taaaccagtg tagaagtaag gatcaaggca
     3961 attaattcga tagtcgacat tatagttcgc ccccttcatg gttttgattc gttggtttcg
     4021 attctattgt cgcaacgatc tcttcttgtt ttttcccttg gaagacttta gccatgacga
     4081 aacgaatcag gaagttgatc ccgacaaaga agataatcaa agcaatcgat acattgacga
     4141 tctcaaacgg aacacctgct cctgtctgca tgccaagtcc accgatcttc aaaatactga
     4201 acaagatcgc tgataaaaga ataccgattg ctgatccaga accaagcaag gaaacagcca
     4261 ttccgtcaaa tccaatagat agcgaagtcg tttgaacaaa gaagttttga tacgttccta
     4321 atccttcaac cacaccgcca agccctgcca aagcgccaga aatgaccatc gacaaaacga
     4381 tcgtacgttt actactcatt cctgcatact cagaagcaaa aggattcaat ccaacagaac
     4441 ggatttcaaa tcctaaagtc gttttcttca tcagcagcca aacgaaaata agcgcgatga
     4501 ttgctaaaaa taatcctgag tttacgcgag agccgccaaa agcttgtgtt aaccatggta
     4561 accgcagtga ggcattttga ctgatcatat tggaagagtc caaacttgag cgatagcttt
     4621 caggcatcac ttcatgtaaa gcaaatgtgc ttacgtataa taagatatag ttcatcatga
     4681 ttgttacgat cacttcactt gttccaaaaa atgctcgaag taaacctgga acagccgctg
     4741 ccatcgcacc agctaaggca cctaagatca cgcataacgg aagcaaaaca acttttggca
     4801 gatctggaaa cgcaagtgcc acccagacac tgacgatcca cccgcaaagt gcttgtccag
     4861 aaagcccaat attgaaaaac ccagctgagt tagctacaga aaaacctaat gctgtaaaaa
     4921 tcaacggcgt tgcttgcctt aacgtttcac ctatccctct ttgcgaacca agggctgcat
     4981 ctagcataga agaataacct tggatgggct gaaaaccaaa agcagccatc aatatcgcgc
     5041 ctaagatcaa tcctaaaaca actgaaagta caggcactaa taaattacgg aatttttcgc
     5101 tgcgatcatt catgtgcgcc agcccccttt tctaactctt ttcgtgcttc ttctaaggta
     5161 tagcccgcca tcaatagacc taattcattt tcagatgttt ctttcggatc aacgataccg
     5221 acaatttctc cttcatggat aacagcaata cgatccgaaa catttaaaat ttcttctaat
     5281 tcgaaactga tcagtaacac tgctttgtat ttgtcccttt gttcgatcag acgtttatga
     5341 ataaattcga tcgcacctac atccagtcca cgagttggat ttgcaacaat caacagatca
     5401 ggatctcggt cgacttcacg agcgataatt gctttttgct gatttccgcc tgataaagct
     5461 tttgcgggaa caagttcatt cgttgttcga acatcatatt cttcgatcaa ttctctggca
     5521 tgttcattca ttactgaata attcagcatg ccgtttttac tgtaaggttt ttgatgatag
     5581 gtttgtagtg cgatattttc agataatgtc atatctagga ctaacccgta tttatgacgg
     5641 tcttctggca cgtgtcctac accatgttca gtgatttttc ggggtttttt attagtgatg
     5701 tcttcccctt ttagcttgat atgtccgctt tctgcctttc gcaaaccagt caaagcttgg
     5761 atcaactccg actgcccgtt tccatcgatt ccagcgatac caagtacttc gccagcacga
     5821 acctctaagt tcaagttttt cacggcttct aatccacgat tttctttcac cactagattt
     5881 tcaatagaca aaacgacttc ttgaggcttc gcttcttttt tcatcgtttt gaatgaaacc
     5941 gttcttccaa ccatcatatc ggctaattgt tgtgaggtta cgtctttaac gttgactgta
     6001 ccgattcctt ttccacggcg gataactgta cagcggtcag ctactgcttt gatttcatct
     6061 aacttatgcg taatcaaaat gatcgacttg ccttctttga ctaattcctt catgatcacg
     6121 attaattcat caatttcttg aggggtcaat acagctgtcg gctcatcaaa aatcagtaca
     6181 tctgctcctc ggtaaagtgt ttttaaaatt tctacccgtt gttccattcc aactgaaata
     6241 tcacgaacgt aagcatctgg gttgactgac aatccatact gttcagaaac tttttggatc
     6301 tcttttcgtg cttttttatg gtcaagcatc cctgcgcgac taggctcgct tcccaacacg
     6361 atgttttctg ttacagtaaa ggcatcaaca agcataaagt gctgatggac catcccaatc
     6421 cctaattggt tggctttggt cggacttgtg atctttactt ctttcccacg catcaaaatt
     6481 ttccctgatg tcggctctaa taatccggaa agcacgttca tcaatgtaga tttgccagca
     6541 ccgttttctc caagcaacgc atgaatttct cctgccttta cttgtaaatt gatgttgtca
     6601 tttgccttga atgttccaaa ttctttggta atattctgca tttctatgac aatattttct
     6661 tgaaccacct ttttttcaca tcctaacgat ttctatctag ggcaacgatt gactaagatt
     6721 cacgcttagt gagcgttctt cttttgaaac aaacattact tcagggagtt gttcccttat
     6781 ccaaactgtc acaaggcagc ttggataaat gaacaattct tgttctaatc aaaccatagc
     6841 cttgtcaaac atgtgtctaa caaggctata gaaatggttt tagttttctt caggagtttc
     6901 tggaactttt acgtcacctg aaatgatttt ttcttttgct tcatcaactg cttttttcgc
     6961 ttcgtcagaa agttgtcctt ctgttagacc aacaccgtct tctttcaatc cataaacagt
     7021 gtgttcgccg ccagggaatt taccgtccgc tgatttttgt gccaaatctt ctactactgt
     7081 tccaactgct ttcagtgtag atgtcaatgt gaagtttttc ttttcgccgt ttaatgtgta
     7141 ttcgccttcg tctgattggt cgcggtcaac accgataacc caaacttttt tgtcgcctga
     7201 ttcgttcaat gattttgctt cttggaatac accgttacct gtaccgcctg aagcatggaa
     7261 gatgatatct gcgttttgtg catacatacc ttgagcaatt gaacgtcctt tatctggagc
     7321 agagaagtca ccagcatatt gattcaatac tttgatttct tttcctaatt ctttcgcacc
     7381 tgcatcaaca ccggctttga atccagcatc aaaacgatcg attacttcac cttttacacc
     7441 gccgataaag ccgactacgt ttgtttcagt cgtataagct gctgcaacac ctgcaagata
     7501 agaagcttcg ttgtctttga aagttgcaga tacaacgtta tctaatccat cgatgacatc
     7561 atcgataata acaaagttcg tacctgtatt gtttgttgct tgttcttgga tcgctggttt
     7621 tagtttataa ccaataccaa aaatcgtttt gaatccagca ttcaatgctt ggtcaatatt
     7681 tgggatgtaa tctgattcat ttgaagattg gaaatattgg aagccatcat ttcctcgtga
     7741 aagtccttga tctttccccc atttttctag tccttcccaa gctgactggt tgaatgaacg
     7801 gtcgtctaca ccacctgtgt ctgtgatcaa tgctgcagtt gttgtgtctg atgagctttt
     7861 gccaccggat gaatcagtag agccgttgtt gccgcatgca cctaaaaata accctgctgc
     7921 tagtgcgaca gcacctaaac caaataattt tgcttttttc atttctgtaa agcctcctaa
     7981 aataattgtt ttatttttca acagtcagct gactgaatga acatgagtta tagatctttt
     8041 tcagtaaatg cataaggcag cagtgcacga acggtcgttg ccttcgtcac accattgtct
     8101 cctactagcg taaccggcat atcaggggaa cagaattctg ccattacttg tcgacatgca
     8161 ccacaaggag agataggatc gggggtgtgc cccgctacga ctagatgcgt aaatgttttt
     8221 tctccttcag atactgcttt gaaaaaagct gtgcgctcgg cacaattcgt taatccaaat
     8281 gatgcattct caatgttgat gccttgataa atcttgcctg attccgttac gagacaagcg
     8341 cctaccggaa aatgggaata aggaacatag gctttatcta acgcttcaat agctgtgttg
     8401 atccattctt gtttgagttc tgccatgagc ttctcctttg aaaaaattaa tagccttcac
     8461 cagttgaacc agatacgatt gctactcctg cgcttgttcc gattcttgta gcaccagctt
     8521 cgatcattgc cattgcttct tcttcgttat ggataccgcc ggaagctttg acacccattt
     8581 ctggtcctac agtttcacgc atcaaacgga catcctctac ctttgctccg cctgttgaga
     8641 aaccagttga tgttttgaca aaatcagcgc ctgcagcttt tgctatttcg caagctcgga
     8701 tgatttcttc tctatctaat aacgatgttt caataataac tttgacaagc gcacgatctt
     8761 ttgctgcttc aacaactgct tcgatatctt tttggacttg ttgatcatcc tttgatttca
     8821 aagcaccgat attgatgacc atatcgactt catctgcacc gttattgatg gcatctgttg
     8881 tttcaaatgc ttttgtctcg ggtgtgtttg ctcctagagg aaacccgatt actgtacata
     8941 cggctactgg ctcaccttgt aattgttctt tcgctagcgc aacccatgct ggattgatgc
     9001 agacagaata aaaatggtat ttttttgctt cttcgataat ttgcatcaca gcttctttag
     9061 atgcgtctgc tttcaaaata gtatggtcga tcattcggtt tagttccatt tcttttcccc
     9121 ttttctttaa aacggtaaag tccgcttttt tcctttattc tgtgatgatg tcatgaatca
     9181 acgttggctt cattgccgat tcaccaattt caatattttt atataataat tcaatgacag
     9241 aagtgatttc ttcgtcgtta ctatagatcg tcaatagtga ctcacctttt gtcacagtgt
     9301 caccaatttt tttatgcagt ttcaatccaa cagcgtgatc aatatcttct tctttggttt
     9361 tacgcccagc acctagcatc attgcagcaa ttcctaattc atttgccacg atttttgaca
     9421 caactccgct ttgtttagca ggaagctcca ttgtgtagcg tgcagtcaaa attcgttctg
     9481 gatgttccac gatcgctggg tctccccctt gattttggat catttctttg aattttgcta
     9541 acgctttacc tgattccaga gcttcttgaa gcaacgttct tgcttcatcg gttgtcttag
     9601 ctttgccagc aagaacaacc atttggcttc ctaaggcgta gcacatttct actagatctt
     9661 ctggaccatt tccttgtaat gtttcgattg cctcgacaac ttctagacta tttccgatag
     9721 cttcgccaag aggttctgac atatctgaga taaccgccac tgtttctcgg ttagccagtt
     9781 tcccaatgct cgtcatggtc tttgccagac gtcttgcatc ttcgatgttc ttcatgaaag
     9841 caccgtctcc agtggtcaca tccaaaacga ttgcatctgc tcctgccgcg atttttttac
     9901 tcataatcga actggcaatc agcggaagag aatcaacggt cgctgtgacg tcacgcaagg
     9961 catacaattt tttgtccgcc ggtgccaagt ttcctgattg accaattacc gctactttac
    10021 tttcattaac aatgcgtaca aattcttcat ccgaaagctc gatctgaaat ccgggaattg
    10081 cttctagctt gtctagtgta ccacctgtgt aacctagacc gcgtccagac attttagcca
    10141 ctgtcactcc gacactagct actagggggg ctaaaatcaa tgtggtcgtg tcaccaacgc
    10201 cacctgtcga atgcttatct acctttattc cttcaagaga actcaaatcg atgacatcac
    10261 cagaattagc aatcgccaat gttaagttcg tgatttcttc atttgtcatg tcttgataaa
    10321 aaattgccat caataatgca ctgacttgat aatctggaat ctttccattc gtatagttcg
    10381 tgacgatgta ctcgatttcc tctttcgaca gctcgtttcc atccctcttt ttttcaatca
    10441 gatccaccat tcgcattgcc tgatcctcct tgtcttcatt cggtattata cattattttt
    10501 aggaaaggta aaccacttta gtaaactggt ttactaaaaa taaaaaaagc actaactagc
    10561 ctttgtaaat gcttacataa gcaatatacc gccgagttag ccctttgtca atacttttac
    10621 gctctcacga ataattaatt ttgtttcgaa cactttgttc ggaacttttc tttgaggaaa
    10681 ttcgatcgta tcgatcagga aacgtgcagc ataaaatcca atatcaaaac ttggctgctg
    10741 cactgtcgtc aattgcggaa tcatgtattc tgacagcttt aatccatcga atcctatgac
    10801 tgagatatct tctgggattt ttttgcccaa ttcatcgatc gcctgatagc atccgatcgc
    10861 catggcatca ttcccgcaaa aaatagccgt gatatcttta tgtttgagta atttttttga
    10921 tgcagtatat ccaccttgga cagtcaactc tccatgttca acaaatttcc cattaaaagg
    10981 caggccatga tctttcagcg catgacgata tccgttgaaa cgttcttcta attgatagta
    11041 tccagtggtt tccttcagca tgccgatctt cgtatggcca ttttcaataa acagcgaaac
    11101 agcttgatag gcgccctcat attcctttac gataagccgt ccgttatccc gacgattcaa
    11161 tccgcgatcg attaaaatga ttggcatttt ttgataaaaa ccgctgtcta atgcatattt
    11221 ctcaggtaaa atatttggtg tggccagcaa gattccgtcc actgagcggt gggaaagctc
    11281 ttgtaaatac ttgatctcat tttcttggct atgacgagaa ttgcataaca tgatcacata
    11341 gcccaaagga ttcaaatagc tctctacacc ttcgatcagt ttggagaaga agaaatcagt
    11401 gacatcaggg acgatcattc cgatggtctt tgaatgtcga ttgatcacat ttgaagcgaa
    11461 ataatcgggc ttataatgat gttcattcac gatcgacaac actttttttc ttgtcttatc
    11521 actaaatctg cttcctttgt tattcaaaat ctgtgagaca gttgttactg atacgcctgc
    11581 catttctgcg atatctctga tggtcagctt catcctattg gctcccttct atctaaattc
    11641 cctatcagtg taccaaaacc cccaagaaaa gaatagagaa aaaaggaaag tatgtttttt
    11701 gataaaaacc tcctttatct accgacttgg cagccttcta tacaagttct gtatcttatt
    11761 tattttatcg taaaaaacaa cgactctgtt cctactattt ctttttgaag aaaaaatagt
    11821 tctttgtttt taaaaacttc tcaacaaaaa aagaactgat acgtacccgt acatgcacgc
    11881 ccagttccac tctttcgtcg ctccactatt tttcagctag ttattctttc acacttttga
    11941 tgatataata tcctttatct tttgtgacga tttctgcatt accaaatgtt tcttccattt
    12001 tcttttgcgc actcggcgct ccttgtttct tctggatcac aattgtcagt gtaccgccct
    12061 tcttcaaccg aggatatgcg cctgtcaaaa tttcatgtac aacttttttt ccagcacgga
    12121 tcggcggatt gcttacgata gctgcatagg tttcttcatg aactgcttca tagatattcg
    12181 attgatggat atcaacatgt tcgatttggt tacgctttgc gtttccttgc gctagactta
    12241 ccgctcgttc attaatgtcg accatttcaa caaaccgctg tgtcgcaaat gccaaagcta
    12301 acccaatcgg tccatacccg catcccacat ctaagatctt tccttcatca ggtaactctt
    12361 cccaatcgaa agcatcaatc aggacacgtg aaccataatc cactgtttca cgtgaaaata
    12421 caccagaatc tgttacaaat tgaaaggttc tgccttttaa ctcaaaagcc cattgttcaa
    12481 aatcgtgcgc cgtatctggg cgttcagaat agtaatgatt tgtcatgtct tccatccttc
    12541 tcatttcttt catgacaaca gtctatcctt ttatgtatcc gtgcgcaagt caagaataag
    12601 actccgtttc tttctattat aaagattata caaaaagatc ggcaatctct cttaagtggt
    12661 ggggtttgcg tttattttgt gttaaaatag ttccattgat gggagaacgc aggaggctaa
    12721 tatgaatgat ccaatgaact actaccgtgt ggcaagagaa gaatggcgag aattttatcg
    12781 taatgggcaa gcacctttga cacaagatga actagataat atcaaaagtt tgaatgaccg
    12841 catttccatg caggatgttc gagatatcta tgtacctctt gctcatctga tttatcttta
    12901 tatgaaagaa tttgaatctt tgttcttgag taaaggttta tttcttcata aatacgtaga
    12961 agtaccgcct tttattatcg ggatcgccgg aagtgtagcc gttggaaaaa gtacaacagc
    13021 cagactgctg cagacgattt tgtctcgtac cttcaaacga agaaacgttc agctgatcac
    13081 gactgacgga tttctttatc caaatcgtat attaaaagag cgtggaatca tggaccgtaa
    13141 aggatttcct gaaagttatg atatggagat gctgatcgat ttcttgaacc gtgtaaaatc
    13201 tggtcaggaa gagataaaag tacctgttta ctcacatgat gtgtatgata tcattccaga
    13261 caagtacgaa ttgatccagc agcctgatat cttgatcgtt gaggggatca atacgcttca
    13321 attacctgcg aatcagcaaa tctacgtcag cgactttttt gatttttctg tctatgtcga
    13381 tgccgatccc gtgctgatcg aaaaatggta tttggatcgt tttggtgcgt tactggatac
    13441 ggctttccaa aatccagaga attattatta tcgttttgct atcggagatc gtgaagaagc
    13501 ctttaaaatg gcaaaagatg tatggaaaaa tgtgaatttg aaaaatttac aggaatatat
    13561 cttgcctaca cgaggaagag ccgatataat tctccataaa actgaaaatc atatcatcga
    13621 ttcgattttc atgcgaaaat attagcattt taaaatagtt tgactatttt gagataaaat
    13681 ttaaagggat aggggtaata aatttgacaa acgttgccga tatgacgaac gttgaaaaga
    13741 ttatcgtatt agactacggt agtcagtata accaattgat cacaagacgt attcgtgatc
    13801 ttggagtctt ttccgaatta ttgagccacc gcattactgc agaagaagta aaagcgatga
    13861 atccaaaggg aatcattctt tctggcggac caaacagtgt atatgatgag aatgcatttg
    13921 ggatcgatcc tgagatcttc aatttaggta ttcctgtatt agggatttgc tatggtatgc
    13981 aattgatcac gtacaaactt ggcggttccg tagagcctgc taaaaaccgc gaatatggga
    14041 aagccttgtt agaagtaaca agcgatcaag ctgtcttgtt taaagaaaca ccaaaacaac
    14101 aaacagtatg gatgagccat ggtgacttgg taactaaagt tcctgaaggc tttgaaacag
    14161 tcggaacaag caaagattgt ccgattgctt ctatccaaga cacaacacgt aatttttatg
    14221 gtatccaatt ccatgcagaa gtacgtcatt ccgaatacgg aatcgaattg ctgcgccact
    14281 ttgcctttga tgtttgtcac tgtgaaggta actggacaat ggataacttt atcgatatgc
    14341 aagtagaaaa aatccgtgaa caagttggtg acaaaaaagt cttgcttggt ctttcaggtg
    14401 gcgttgattc tagtgtcgtt ggggttcttt tacaaaaagc gatcggtgac caattgacat
    14461 ctatctttgt tgaccacggt ctgctacgca aaggcgaagc ggaacaagta atggaaagtc
    14521 taggcggaaa attcggcttg aacatcatca aagtcaatgc aagagatcgc ttcttagaca
    14581 agttgaaagg tgtatctgac ccagaacaaa aacgtaaaat cattgggaat gaattcgtat
    14641 atgtttttga tgacgaagca acaaaacttg ctggtgaaga aggcgtctct ttcctagcac
    14701 aaggaacgtt gtatactgac gttatcgaat ctggtacaga aacagcacag acgatcaaat
    14761 ctcaccataa tgtaggtgga ctgccagaag atatgcaatt tgaattgatc gaaccattaa
    14821 acacattatt caaagacgaa gtccgtgaat tagggactca gttaggcatg cctgattcaa
    14881 tcgtttggcg tcagccattc cctggtcctg gtcttggtat tcgtgttctt ggcgaaatca
    14941 ctgaagacaa gctagaaatc gtacgcgaat ctgatgctat cttgcgtgaa gaaatcgcag
    15001 ctgctggact tgaccgtgat atctggcaat atttcactgt tcttccaggg atccgctctg
    15061 tcggtgttat gggtgacgga cgtacttacg actacacagt cggtatccgt gctgtaactt
    15121 caatcgacgg aatgaccgct gactttgccc gtatcccatg ggaagtcttg caaaaaatct
    15181 ctgtacgtat cgtaaacgaa gtagcccacg tcaaccgtat cgtgtatgac atcacttcta
    15241 aaccaccagc aacagtggag tgggaataaa gagccaacat aatatggagg gggaatccct
    15301 ataaatcaac attcttcaat agctaaaatg cttgattttc attaaaaata agatggttcc
    15361 ctaccaaaag agactataat ataagttaat ttgtttcatt cttatcataa aaagaagttc
    15421 tcgaaaaacg aactgttttc cgagaacttt tttctatcca tctaagtgct caaatcatat
    15481 tcacgacgga attctaaggt taccctttct taaacaaact ttatgttatc tgcacaactt
    15541 tttgcaattt tttcattgct tcttacgaaa tcgttgaact ctctttagca taatacaaag
    15601 tgtagatgaa gtctggtgct ttactcgttt tggtaaaaat gactggaaat cgtttctggt
    15661 tatttttggc aaaggcgagg tgcaggttcg attcctgcct tctacgctaa cgatgtggct
    15721 gaagggaacc tccgatcttg aatcgaaacg atccgcagtc tattgtttca ccaatgaaac
    15781 aaaatcaacg ttcgtccaag catttcctga agccttgcta taaaccccaa tttcacaaac
    15841 acctgttgta attggaatgt ctttgattgt aatttttgtc cattcacttg ttttaacaac
    15901 cgcagtattt agttccgctc caccataatt tttgatatat aactgattag tatcaaactc
    15961 tcccccagac attacatagg cactcaatgt gtaagttcct ttgcttatgc cactaacatt
    16021 ttgataagct gatgcctcgt atgcttgatc ctgccaaaaa gttagtttgt agtccccaaa
    16081 aaaagtgccc gcttccgttt tgatcgtacc cttagcggtt ccttgttgaa accaggtttg
    16141 ccaattggaa ggactggtcg ttggtccatc gctttcgaaa cttgcatttt taactagatt
    16201 tatttgcgcc cctgcttgtt taaacgcacg caagcttggc aaggcgtttc cagcaaaatc
    16261 aaacatcgct tggttgtccc attcgtttcc catagtggat tgaacactga gataattcat
    16321 acctgcttgt gttgcccaac tcacatttcc gttgtaccaa agaggttccc aatagaagaa
    16381 gcccgccccg cgattattcg gtactgcctg aatccgatcg gcaagatcca gcaaatactc
    16441 tgcttgtcct gtcggtgttg gttcatatcc ccccgcagct gcttcggctt caccaaaagc
    16501 atttggcata gtatcggcat tcgctgttgt atgcccataa gctgtttcaa ctacgatcac
    16561 ttctttacca taccgttggc tgatcgcatt catattggtt tgaagttcat tcattgaacc
    16621 gtgccaatac ggataatacg acactccaat taaatcaaat gacacacctc gactcgtgat
    16681 ttcatcaaac caccaagtaa atgctcctgt atcgccacca tcagctaagt ggagcattac
    16741 tggagttgtt ttaggttgag tgtctcgaac agcttggata ccagacttca aaaaagcagc
    16801 caatcgatca aattctcccc cgccttctcc ccaagacttg ccataaggcc agagcattcc
    16861 gctattcagt tcattgccga tctgaaccat atcaggataa acgtccagct gcttcatttc
    16921 cgacagaacg ttctttgtat agtcgtacaa tgccgtattc atttcttcaa aggtcagacc
    16981 ttgccatgct ttaggaagat tctgtttccc aggatcaacc caaaaatcac tgtagtgaaa
    17041 atccagcagc accttaagtc ccagattttt tgctcgtttt gcaaggtcaa tcgtcgtttc
    17101 cagatcattt gtccctgctc cataactttc tccattacta ttataaggat cctgccacaa
    17161 acgcagtcga acatagttga caccattatt ttttaaaatc tctaaggcat cttttgaaat
    17221 atcgtcttca taatattttg caccagattt ttccatatct gacaggatcg aaatatctgc
    17281 tccgcgaata aattcgctgg cagatgcttc aacagttttt gaaacagccc ccaaagcccc
    17341 taacacagct gcaaataaaa cagctttacg caaccaaatt cccattttca ttatggctcc
    17401 tcctttattg aaattggttc agaataaact gaacagcttt ttcaggatct ctcgttaatc
    17461 cgatatattc tgctcctttt gtcgcaacca gcttgattcc taagcgatcg gtgtcttctt
    17521 tgatatcatc atagtaagaa ttgacttgcg gtgctaagac caccatatca aaatcctgca
    17581 gcatttcgta atgagaacca taagcacccg ccattgcact gatcggtgct ttcgattcag
    17641 atgcaccttt ttttaaagca ttagctaaca tcccacttgt ccctgcgcca gcacaaagta
    17701 ccaacacatt gatttcttta tttgcatcgc tgataacttg actcgctgcc acaggtactt
    17761 cctcacgtac atcggcagaa gcaccttcct cggttgcgat cacctctgct ttttgtgctt
    17821 ctttctctac taagacagca tcgtatgctt tgcaaaatgg tagatagatc aggaagtcaa
    17881 cgacgattag aaccgctgct agaacaaaag ctaagatacc catgcccgtt cctagaacca
    17941 aaccgatcgg tgctggagtt gcccaaggca aaacgtacat aaagctgttc atgcccaata
    18001 catcgacaaa aaacttgaac aggcaaacgt ttacaacagg tgttaacaaa aatggaataa
    18061 aaaaataagg gttcaaaatc atgggtgccg caaatagcaa tggttcgttg acagcgaaac
    18121 ttactggaat aaaggaagct ttcccaactg ccttcaactg cttagactta gcaaagagta
    18181 ggaagataaa aggaacaacc aatgttgccc cagttcctcc catagttcct acaaaattcc
    18241 ctaaacccac cgtcagtact ttagaagctt gttcaccgga attgaacaat tgcagattgg
    18301 tttccacatt ggcatagata attgccgcaa ttgccggctc cacgatcgac ggtccatgaa
    18361 caccgacaaa ccaaaacagc gccatcgctc cccagataat agcgattcct aagtacccgt
    18421 cagctgctgt aaatagcggc tgcagcaccg taatgattgc ttctgcaaat gagatttgga
    18481 agatttgtcg aatgcctaaa tcaattaaaa cggccgcaaa tactgaaaaa gagaaaggaa
    18541 aaatatctcg gaaggtttgc gaaatcgttc caggaacttc tttaggcatt ttaatggtaa
    18601 tatctttcag cacacaaaac ttatataaat taactgttaa aaatgcagcg acaaaggaag
    18661 ccagtagtcc ctttgtcccc atatattccg ttgaaaatcc accttcaatc ggagagacgc
    18721 ttaacaacag gaaacttacc acagaggcca gcatcacaga ggtggtgttg attactttgt
    18781 ttttcggtaa gcgacgattc atcgaatcag ccaagcagcg agcagtggtt gctgaaacga
    18841 gtaaccctac aacacccatc gaataattat agactttcca tagccatgtg gagacagatt
    18901 caggccattg gtaaccaaaa atctcaggaa ccgcagcaat caaaatgaaa atgcttgaaa
    18961 ataaaatagc tggcatcgct gttaaaaatc cgtcacgaac ggctcctaaa taactattac
    19021 gtgagatttt ttcaaaaaat ggtttccctt tttcgatttg tttaatgatt ccatccatag
    19081 cgttcttctc ctatccttga ttctgtctgt acagtacaat aaagtcttga atcagatctc
    19141 tcagcaataa agtggtcatt aaatgatcct gcccatggat aaaaatgaat cccatctcca
    19201 tctctttccc agatgcttct tccgctaaaa tttttgtttg cgaattatgt gcaagggtta
    19261 aattttcgtc ggcttctttc aacgccgaat caacatgttc aaagtttcct tgtcttactt
    19321 ctttcaataa tttcaataat gtactgcgag cgtcgcctga atatgcaacg atctcaaaac
    19381 caagcatttg taattcctct ttattcatct tttcttcctc cattatcact tattaaataa
    19441 tcgttttggt ttcactgacc aacttgtacc agtaagctga ttcttttggg taacgcttct
    19501 gggtttcaaa atcaacataa aacaagccat accttttgtt atacccattt gtccatgaga
    19561 aaagatccat aagcgaccac aaaaaatacc ccttcacatt taccccggct gttatcgcct
    19621 tgcttaagga ttccaaatag actctcaaat agtcgattct cggctgatcc ataataatgc
    19681 catcttcaaa ctgatcttta taccccatcc cgttctcagt gatatagatt ttgttgtaat
    19741 gtgggtaatc gcttttaatt cgcagcaaca agtcatatag accttctgga taaatcagcc
    19801 agtcccaatc agttcgagga attccttctt tataaatacg ttctccgatt cccttcactt
    19861 tataaacaga ggtccctttt tcacccgtac cgttatgatg gattgcattt tctccatcgt
    19921 aagctttgac aaaatggcat tggtagtgat tgatccctaa gtagtcatta cgcgtggatg
    19981 cttttttcaa ttcaacgaaa tcttcttcag gaaaatgata gctggcctga tttgcttcac
    20041 atatctcatc aagagcagtc aaggtttcag ttgaataata gcctaaatag gtagcatcta
    20101 ataagaaacg gatcgacaat gcatcgtcta aaaaggcagc atgcttatct tccggcgcgt
    20161 ctgttgccgc atatttcgtc tctaatgagt gaactacacc aatttcaccc ggaagttcgt
    20221 tttctttgaa atagttcact acacgggcat gagcaaccat catattatga agacaggcga
    20281 cgatctttgt aaaatcatat ttgattcctg gtgggaaaac acctaacaaa tattgattgg
    20341 ttgcaactgg atagatttca ttaaaggtac tccacacctt gacttctttg aattcatgaa
    20401 aacaaaaaat ggcataggaa acaaatgctt ctattgtttc tcgattcaaa aaatctccat
    20461 ggtcaaacaa acgtttaggg gtatcaaaat gatgcaacgt cacaaacggc gttacgtttc
    20521 ttttcttaca ttcttcgaac acacgatgat aaaaagcgat tccttctgga tttggtttcc
    20581 cagctccatc aggaaaaatg cggctccaag caatggataa tcttagacca tttacaccaa
    20641 aacgttcaca caattcaatg tcctttgcat attgatgata aaaatcactt gccggatcgg
    20701 gactgaatcg cccttgttcc tctaaaaagt catcccaagc aacagcgcct ttgccacctt
    20761 ccttagtggc tccttcaacc tgataggctg ctgtggctcc gccaaaaata aaatcttctg
    20821 gtaatttaag catgtacatc tctcctcaaa tctataatca gttttcgctc tttctaactg
    20881 attgcattgt aaacgcatcc aaaacatttg tcaacaaaaa caaacacaaa taatcataaa
    20941 aaccagaaaa cagcaataaa atctattgta acaaacataa aaaccaccct aaatgtttac
    21001 ttttgttcga attagattga ctgttgtccg tttatatgat agattatcct taaattagag
    21061 gtgaagacaa tgttgaaaaa agaaagattg cttactatcg tagaaatggt caataaaaaa
    21121 ggaatcctta ccgttaatga aatcatcaat caattagatg tttctgatat gaccgttcgc
    21181 agagatttag atgaattaga aaaatcgggc aagctgctgc gtgtccatgg aggggctcaa
    21241 agtctttcct acactttaga tcaagaactt tctcacactg aaaaagccac tctgcagatt
    21301 caagaaaaaa aagaaattgc tgccacagca gcctccttga ttcacgaggg agaaacgatt
    21361 ttcttaggac caggtacaac aatcgaacta ttggcgacgc atttactgaa caagaaaatt
    21421 cggatcatta caaataatta tccagttttt gatattcttg ctcaagcaga tggaccagaa
    21481 attctactga ttggcggtga tttccggaaa aataccggcg catttgtcgg accgatcacc
    21541 aacgacaatt taagaagact aaagtttaca aaagcattta tcagttcaaa tggcgttcac
    21601 aacgaagcga tctctactta cagtaccgaa gaaggagaag cacagcagat cgctctaaat
    21661 aattcccgta agaaatatct actcgttgat aataaaaaat tcaatcgaga tgatttttat
    21721 gtgttttata atttgcatga tttcgatctg ctcattacgg atcgccagat ctcgcgggag
    21781 gttcaagagc actatgcaca atatgtcgat ctgcaaattg ctgattcaac cgaaaatacc
    21841 actataggag cgtaactatg aaagggatta tatttgattt caacggaaca atgtttttgg
    21901 actctcatct gcatgaagct gcttggctgc atatgataca tgatcataca aaagatggtt
    21961 tatcagacga ggatatttta aaaaatattc atggtcggac gaatacggag attttgaaac
    22021 atttcatttc agagcatttg accgcctctg agatcgccgt attgtcagaa gaaaaagaaa
    22081 gctattatcg tatgctttgc ttgcaaaatg aaaacgagct tcagttgaca aaagggcttg
    22141 aagcgactct tgaccagctg actgaccaac agattccatt aacgattgcc acggctactg
    22201 tcaaagagaa tgttgctttt tattttgata ttttcgattt agcccgctgg tttgactggg
    22261 acaagattgt ctatgacgac ggctctttcc ctgggaaacc gcaaccggat atttttctta
    22321 aagcggcaga caaactgagc ttagctccgg ctgattgtct tgttatcgaa gatgcttact
    22381 caggattact tgctgccaaa cgtgcgggga tcggaacaat tattgcaatt gatccattcg
    22441 gtaaaaacaa aacagtattt gaacaggcac agctaaataa agatggaacc atcaaagatt
    22501 ttactggttt ttggacagag cacctgtctg ccacatccgt taattcttaa acgattcaac
    22561 taactaccaa agaaaagcaa ctgaaatgca cacctccttt tataaaaaac tgccagtgaa
    22621 acgacatgaa agatacactg ctatatcctt agtgaaatat agcagtgtat ctttgacttt
    22681 ttcattcttt taaccatatg acgcgttcat ctctatctat aattaacatt aaaaatagtg
    22741 ttttcacatt atttctcaaa ccaatcttaa aaatcatcag gagtactttt ttgatatact
    22801 tttattaatg ctgggcgtaa tggttaaaag atttccacta gttcctctcc accaagctga
    22861 gttgaaaaat gcttatctgt aaacaacata gacaaagagg aaataaacgt ttccgtatct
    22921 ttctctgtcc caaggatatt aattaatttt cctactaggt aagtatcatt aaacttaaga
    22981 tctgccttca catcaaaagt tttgataaaa gcttttatca tgcgtacaat tttgtcttta
    23041 tcgccataat tgatttcacc actcatatca atatagtcac taatccctga agaaataatg
    23101 gcaactgctt cttctttcgc cttatcagat aattcattta atgactggga ttgattgatg
    23161 acttcttgag ttttatctgt tacgtaatct ctcattatcc tatcggcata ctccgcaaag
    23221 taatcttgaa aaaattcttc tacagggtaa ccaacttcgt tttcccaacc aggagaaatt
    23281 tcattatcag tacttattaa atctattaaa aaccaacccg ttctctgagt gttga
//
LOCUS       DAZBTF010000043        22790 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000043 DAZBTF010000000
VERSION     DAZBTF010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.043"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(122..499)
                     /locus_tag="ACHGNS_001731"
     CDS             complement(122..499)
                     /locus_tag="ACHGNS_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937674.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            755..1483
                     /gene="purC"
                     /locus_tag="ACHGNS_001732"
     CDS             755..1483
                     /gene="purC"
                     /locus_tag="ACHGNS_001732"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFN6937675.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1483..1737
                     /gene="purS"
                     /locus_tag="ACHGNS_001733"
     CDS             1483..1737
                     /gene="purS"
                     /locus_tag="ACHGNS_001733"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFN6937676.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1739..2410
                     /gene="purQ"
                     /locus_tag="ACHGNS_001734"
     CDS             1739..2410
                     /gene="purQ"
                     /locus_tag="ACHGNS_001734"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFN6937677.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2411..4633
                     /gene="purL"
                     /locus_tag="ACHGNS_001735"
     CDS             2411..4633
                     /gene="purL"
                     /locus_tag="ACHGNS_001735"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFN6937678.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4618..6057
                     /gene="purF"
                     /locus_tag="ACHGNS_001736"
     CDS             4618..6057
                     /gene="purF"
                     /locus_tag="ACHGNS_001736"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFN6937679.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6080..7132
                     /gene="purM"
                     /locus_tag="ACHGNS_001737"
     CDS             6080..7132
                     /gene="purM"
                     /locus_tag="ACHGNS_001737"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFN6937680.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7129..7707
                     /gene="purN"
                     /locus_tag="ACHGNS_001738"
     CDS             7129..7707
                     /gene="purN"
                     /locus_tag="ACHGNS_001738"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFN6937681.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7704..9239
                     /gene="purH"
                     /locus_tag="ACHGNS_001739"
     CDS             7704..9239
                     /gene="purH"
                     /locus_tag="ACHGNS_001739"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFN6937682.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9255..10529
                     /gene="purD"
                     /locus_tag="ACHGNS_001740"
     CDS             9255..10529
                     /gene="purD"
                     /locus_tag="ACHGNS_001740"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFN6937683.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10651..11565
                     /locus_tag="ACHGNS_001741"
     CDS             10651..11565
                     /locus_tag="ACHGNS_001741"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFN6937684.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11698..13263)
                     /locus_tag="ACHGNS_001742"
     CDS             complement(11698..13263)
                     /locus_tag="ACHGNS_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6937685.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13256..14707)
                     /locus_tag="ACHGNS_001743"
     CDS             complement(13256..14707)
                     /locus_tag="ACHGNS_001743"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN6937686.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14721..15737)
                     /locus_tag="ACHGNS_001744"
     CDS             complement(14721..15737)
                     /locus_tag="ACHGNS_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN6937687.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15957..17045
                     /gene="chvE"
                     /locus_tag="ACHGNS_001745"
     CDS             15957..17045
                     /gene="chvE"
                     /locus_tag="ACHGNS_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFN6937688.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17099..18625
                     /gene="mmsA"
                     /locus_tag="ACHGNS_001746"
     CDS             17099..18625
                     /gene="mmsA"
                     /locus_tag="ACHGNS_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFN6937689.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18627..19832
                     /gene="mmsB"
                     /locus_tag="ACHGNS_001747"
     CDS             18627..19832
                     /gene="mmsB"
                     /locus_tag="ACHGNS_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFN6937690.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20089..21066
                     /gene="guaC"
                     /locus_tag="ACHGNS_001748"
     CDS             20089..21066
                     /gene="guaC"
                     /locus_tag="ACHGNS_001748"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFN6937691.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21330..22754
                     /locus_tag="ACHGNS_001749"
     CDS             21330..22754
                     /locus_tag="ACHGNS_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFN6937692.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
BASE COUNT         7342 a         3843 c         5159 g         6446 t
ORIGIN      
        1 gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc aataatagga
       61 acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa aaatagagga
      121 attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga tgttgcagca
      181 agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg atacgataat
      241 gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct ttgtgttctg
      301 tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct cggcggattt
      361 ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg atcgacagca
      421 aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa agctgacctt
      481 gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa atttttagtt
      541 aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta taagattttc
      601 gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca tatttttgtt
      661 gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa aacgatgtat
      721 ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac ttttgtatga
      781 aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga tagaatattt
      841 agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa aaggagaatt
      901 gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa taaaaaatca
      961 ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa ttatccctct
     1021 ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag gtgttcccga
     1081 aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc agttggatga
     1141 tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg aagaaatcag
     1201 tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat ttgctaaggt
     1261 cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac aaattttgct
     1321 tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga atgaacatat
     1381 ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc aagaagtata
     1441 taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt cgtaaaagta
     1501 tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa aaaagcagtc
     1561 catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt tgagatacaa
     1621 cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga caagctgctt
     1681 gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga agtataacat
     1741 gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt tatgggcagt
     1801 tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct ctttagaagg
     1861 atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga gatgcggtgc
     1921 gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag aagggaaacc
     1981 agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt tgccaggtgt
     2041 actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga aagtcaaccc
     2101 caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc cagttgctca
     2161 tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa aaaataatca
     2221 gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg cagggattac
     2281 gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag tagaagactt
     2341 gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact tcggaaaggt
     2401 gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga aaaaaagaat
     2461 ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag atatcttagg
     2521 aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg aacattgttc
     2581 ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc atgtgttgca
     2641 aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag ttgtcttcaa
     2701 agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg cgacaggagt
     2761 tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt tgctagattc
     2821 attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag aagtagttgc
     2881 aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg aaattgcatt
     2941 tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt tgattgaaca
     3001 caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt atgtaggtgc
     3061 aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat ttaatcaaga
     3121 agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa agctgctgct
     3181 tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta tacaggatat
     3241 gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg gctcaggact
     3301 tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt atgaaatgat
     3361 gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg aagaaaaaat
     3421 ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag taacagacga
     3481 cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag tcgatgcatt
     3541 agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa tcgtagaatt
     3601 tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat tgcttgatct
     3661 gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg attcacaagt
     3721 acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg ttagagggac
     3781 gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt tggatccaaa
     3841 aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta gcggtggtaa
     3901 gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag aagttttttg
     3961 ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag gaactcctgt
     4021 tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct atccaacacc
     4081 aatgattggt atggtcggat tgattgagga tcataaacat atcactactc aggaattcaa
     4141 aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg atggcagtga
     4201 attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg atctatccat
     4261 tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga ttgaaagtgc
     4321 tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt tcaaacatca
     4381 gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag aaactcaatc
     4441 aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa tgatgggaga
     4501 tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt cagcaactga
     4561 cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg caatcgtatg
     4621 tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg gggtcatcca
     4681 gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg acaagaaggt
     4741 gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt ggggctgctt
     4801 tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc tgcaatcgga
     4861 catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc ttttctattc
     4921 aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa tgctcgttcc
     4981 cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga tacggagata
     5041 ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt aaaaagtgct
     5101 ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc aatgattgcc
     5161 gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa cggagcatac
     5221 gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat aagagatgtc
     5281 cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg ctttacgaag
     5341 gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc agattcaaac
     5401 atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt agcgaaagaa
     5461 tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc agccgcaagc
     5521 ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa tcaatacatt
     5581 gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg aatgaaatta
     5641 tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga ttcaattgtt
     5701 cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc aaaagaagtc
     5761 catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat tgatattcaa
     5821 acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga atgtatcggt
     5881 gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg tctggaaaaa
     5941 gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc gacgccgtta
     6001 tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt cttttaaaag
     6061 cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag ctggcgtaga
     6121 tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa aaacggaacg
     6181 tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat cggtctttca
     6241 attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat tagcagtggc
     6301 aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt gtgtcaatga
     6361 tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa cggggaaaaa
     6421 tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg ttcaaggaaa
     6481 tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag gtaatgatta
     6541 tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga caggagaaac
     6601 gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt caaatgggta
     6661 ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat caaaactgcc
     6721 agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct atgtttcttc
     6781 cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca ctggtggagg
     6841 gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa tcaaacttgg
     6901 ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa ttcctgagat
     6961 ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc aagaatcgaa
     7021 agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg aaatcgggaa
     7081 agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat gagaatcgct
     7141 gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct atcgaaaaaa
     7201 ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata tgttttgaaa
     7261 cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt tgattcaaaa
     7321 aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga tttgatcgta
     7381 ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga taaaagaatc
     7441 atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat acgtgatgcc
     7501 tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca aggtgtagat
     7561 actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac gttcgattct
     7621 ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat tagtgaaatt
     7681 attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca gataaaactg
     7741 ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt tcaacaggag
     7801 gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa gaagtgacag
     7861 ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc catggtggtc
     7921 ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa aatatccaac
     7981 caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg aaaccagatg
     8041 tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg ctccgtagtg
     8101 cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat gaagaagtac
     8161 tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa ctagcggcaa
     8221 aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta acggattggg
     8281 tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa acgcttcgtt
     8341 acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg gaaaaatatt
     8401 cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat atccgagatg
     8461 cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc gccttgaaac
     8521 acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg gaagcagcgt
     8581 atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga gaagtagatc
     8641 tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc ccttcttata
     8701 cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg acagtagact
     8761 ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt ggacttcttg
     8821 tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg acaaaacgta
     8881 agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc aaacatgtga
     8941 aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga gccgggcaga
     9001 tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa atggaaggtg
     9061 cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat gcagccaaac
     9121 atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat tctattgata
     9181 tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt agacattaga
     9241 gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa catgcaataa
     9301 gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca ggaaatccgg
     9361 ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat tcagcgttaa
     9421 tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa gttcctttgt
     9481 taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt ccgacaaaag
     9541 cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat acttatcaaa
     9601 tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg tatatcaaag
     9661 aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa ggtgtagtcg
     9721 tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac cagcatcgat
     9781 ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa gaattctccc
     9841 ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa gatcataagc
     9901 gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat tcaccagtac
     9961 cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa ccagcggctt
    10021 tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt ttgattgcta
    10081 cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct gaaacgcaag
    10141 tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta ttagagaatc
    10201 gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg gttgcagctg
    10261 ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg gaagaagagc
    10321 tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga gcaggaggac
    10381 gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa aaaatttaca
    10441 ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt ggagaaaaag
    10501 ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa aaaatacaac
    10561 aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt ggtacacttt
    10621 tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg tacttctgat
    10681 cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga agatccagta
    10741 aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc agatatttat
    10801 ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc aatcaaacga
    10861 gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt tgatttttct
    10921 aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat ggattatgtg
    10981 gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt agctgaagcg
    11041 tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa tcaaaaacca
    11101 atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa tcagctgcaa
    11161 tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat gacagatgaa
    11221 gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga tatgacgatc
    11281 caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg gaacgaaaaa
    11341 ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac gacaccaact
    11401 ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat cgccggtacg
    11461 atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat gtctcgggaa
    11521 gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt gtcataagca
    11581 ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt cagcttcgag
    11641 atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt tcgtttatta
    11701 gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa tcccttgttt
    11761 tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa aagcaatatc
    11821 tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc tttgttctgt
    11881 cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga aatgagaggg
    11941 acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg taaaattttc
    12001 tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg aaggctgtga
    12061 gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa gcgagtcgtc
    12121 ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt gcgggatttt
    12181 cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac gatataatcg
    12241 tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag atacttcgtt
    12301 tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat caactggaag
    12361 tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg aactggcggt
    12421 ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc tttctgcgac
    12481 aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat ggaccaacgt
    12541 ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa ctattaaaaa
    12601 tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc taaataaaaa
    12661 cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt ggtttgctgc
    12721 taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag caagtttcaa
    12781 tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat tttttttcaa
    12841 ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta aagcagttac
    12901 tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca gataatcatc
    12961 tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac taataaatat
    13021 gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc cgtccatgaa
    13081 aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg aagacagtgc
    13141 catctcacca tctccagctt cgccgacata agtgattgga taataagaac caaggacagt
    13201 tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa aaactttata
    13261 catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg acagttgttc
    13321 cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa taaagaagga
    13381 gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg atccctttgg
    13441 ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg ttgtctataa
    13501 ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat cctttgtgac
    13561 ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag atcattttta
    13621 aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc atatggccgt
    13681 atcttatttt taagatgact agataatcat tgatatggct aatttctttt tcaagaggaa
    13741 tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca tgggtcatag
    13801 tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc aaactgttat
    13861 aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt gctaaatggt
    13921 attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt ttattcatgc
    13981 tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag ccaaggttcc
    14041 ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg acctttctct
    14101 ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact ccaagttcca
    14161 ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt gctaagtcga
    14221 tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca gaatctacct
    14281 gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta ttgatcagct
    14341 cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac gtactaattt
    14401 ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt tcttctagga
    14461 tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc cacatacttt
    14521 ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata tttgcgatcg
    14581 tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc aaagggataa
    14641 cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg cttaaatttt
    14701 ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat attctgctgg
    14761 gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt ttgaatattt
    14821 ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt acactcgact
    14881 acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc atcaatagtt
    14941 acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc taaagcaccg
    15001 tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc atttgtttct
    15061 aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac aattcggtga
    15121 acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt tggtttacga
    15181 ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt ctctgaacca
    15241 gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt gctgacaaat
    15301 aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac gaaaagctcg
    15361 ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc tcgctttgct
    15421 tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat catatccact
    15481 ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat gtagccattt
    15541 cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc agtatgcttt
    15601 ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg gtcaggtgct
    15661 tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag ggctaaaaaa
    15721 aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta tcttatcaga
    15781 taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa acttaaaaaa
    15841 atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa aaaatcaatg
    15901 ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga taaaaaatga
    15961 aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc attctattaa
    16021 gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga atcgctatgc
    16081 cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg gaattagaaa
    16141 aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat caagtagcac
    16201 aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct atcgatggtt
    16261 cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg atcgcctatg
    16321 atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat aattttggag
    16381 taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa ggaaaaggac
    16441 cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta atcaattaca
    16501 acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc gtgccatctg
    16561 gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg gcgcaagcaa
    16621 ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct gttttgtcgc
    16681 catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg tatgggagtg
    16741 aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc gttaaatcga
    16801 tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta gcaaaaaata
    16861 cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat gatactgaaa
    16921 cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta tctgtcgata
    16981 aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc gatctgaaaa
    17041 attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga taagaattat
    17101 ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa tccgagcgtt
    17161 agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg gagaaaacgg
    17221 agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg gctcctatga
    17281 cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag atagtgaaga
    17341 aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt caataaaaga
    17401 gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg aactgacaga
    17461 aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga atcttctagt
    17521 atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat tatcaaaatc
    17581 cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag aaagcgcgaa
    17641 tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca ttatttcaca
    17701 taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg atggacagac
    17761 gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg gaatggtggg
    17821 cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag tttattttaa
    17881 agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa acgaccacat
    17941 caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg gagcgggcag
    18001 aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat ccggacgtat
    18061 attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg caaacggact
    18121 cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg atattcggga
    18181 aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag ataaagaaaa
    18241 agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa actccatcca
    18301 tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat ccaagtggct
    18361 gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag atgttggggc
    18421 aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag ctgtctgtct
    18481 tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata cgatgaacga
    18541 aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct tgatgaaatt
    18601 aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg gataaaaaag
    18661 agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac agcatggtga
    18721 tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt ttctggcgtc
    18781 cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta gctgcaggga
    18841 tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg atggcgtttg
    18901 ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg atgacagtta
    18961 tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg gtagcttatg
    19021 cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt ggactgacac
    19081 aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa aaaatgtcta
    19141 cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca ttgattttag
    19201 gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat cgtcggaaaa
    19261 atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta tttacagcag
    19321 tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg attattttga
    19381 tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca gtagcaggtc
    19441 gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg atcaaaacaa
    19501 agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta gctggattag
    19561 ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg gaactggatg
    19621 caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga acaatcatgg
    19681 gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg attttaggcg
    19741 tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc gttgtgctag
    19801 acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca gaaaaaagac
    19861 tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca ttgtttcgtt
    19921 tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc tttttgctct
    19981 tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc ttgattaaat
    20041 taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat gaaagtattt
    20101 gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg ttctgaatgt
    20161 gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc tgccaacatg
    20221 cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta tttctatatc
    20281 atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa aaagcgtgga
    20341 ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat agagaagttg
    20401 gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg acatgctaac
    20461 tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt tgtgattgcc
    20521 ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc agatgcaaca
    20581 aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg ctttgggact
    20641 ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa gccgatcatc
    20701 gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt tggtgcgaca
    20761 atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga aacaaaagtc
    20821 gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca aaaaggcgaa
    20881 aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt gaaagataca
    20941 ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg aagagatctg
    21001 gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa tggagacaca
    21061 atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca ttaagtaata
    21121 agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt attatcgcag
    21181 atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt tttgtcacag
    21241 tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta aaatcaaaag
    21301 aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa aaacaaggac
    21361 cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga aggatcaatc
    21421 ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg aatcgttcca
    21481 ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc aaacgtggca
    21541 gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga atcgattggc
    21601 ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa ataagagaag
    21661 caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg ccagatgagc
    21721 ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg gaagaaaaag
    21781 aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat ttggaaagta
    21841 aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt cagcaagcaa
    21901 tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta gagtcacctt
    21961 ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt gggataccga
    22021 tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa aataaattga
    22081 aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg agtcaaaaac
    22141 gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt gaagacgatg
    22201 tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga acgattgatc
    22261 cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca acgaaaatcg
    22321 gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa gaaatgatgg
    22381 agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat gaagcaattg
    22441 atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg ataagcgctt
    22501 ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac tatgcctgga
    22561 ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta gatatgggtc
    22621 ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga atcaattaca
    22681 gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt ctagaaaagt
    22741 ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct
//
LOCUS       DAZBTF010000044        22634 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000044 DAZBTF010000000
VERSION     DAZBTF010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.044"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(6..812)
                     /locus_tag="ACHGNS_001750"
     CDS             complement(6..812)
                     /locus_tag="ACHGNS_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937693.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            950..2464
                     /locus_tag="ACHGNS_001751"
     CDS             950..2464
                     /locus_tag="ACHGNS_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFN6937694.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(2867..3850)
                     /locus_tag="ACHGNS_001752"
     CDS             complement(2867..3850)
                     /locus_tag="ACHGNS_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFN6937695.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(3855..4502)
                     /locus_tag="ACHGNS_001753"
     CDS             complement(3855..4502)
                     /locus_tag="ACHGNS_001753"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFN6937696.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(4587..5219)
                     /locus_tag="ACHGNS_001754"
     CDS             complement(4587..5219)
                     /locus_tag="ACHGNS_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFN6937697.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(5235..5786)
                     /gene="coaC"
                     /locus_tag="ACHGNS_001755"
     CDS             complement(5235..5786)
                     /gene="coaC"
                     /locus_tag="ACHGNS_001755"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFN6937698.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(5783..6520)
                     /locus_tag="ACHGNS_001756"
     CDS             complement(5783..6520)
                     /locus_tag="ACHGNS_001756"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFN6937699.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(6517..7506)
                     /locus_tag="ACHGNS_001757"
     CDS             complement(6517..7506)
                     /locus_tag="ACHGNS_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFN6937700.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            7764..8306
                     /locus_tag="ACHGNS_001758"
     CDS             7764..8306
                     /locus_tag="ACHGNS_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFN6937701.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            8323..9408
                     /locus_tag="ACHGNS_001759"
     CDS             8323..9408
                     /locus_tag="ACHGNS_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6937702.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            9420..10226
                     /locus_tag="ACHGNS_001760"
     CDS             9420..10226
                     /locus_tag="ACHGNS_001760"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6937703.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            10227..11054
                     /locus_tag="ACHGNS_001761"
     CDS             10227..11054
                     /locus_tag="ACHGNS_001761"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6937704.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            11051..12124
                     /locus_tag="ACHGNS_001762"
     CDS             11051..12124
                     /locus_tag="ACHGNS_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN6937705.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            12552..15248
                     /locus_tag="ACHGNS_001763"
                     /pseudo
     CDS             12552..15248
                     /locus_tag="ACHGNS_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
     gene            15313..15813
                     /locus_tag="ACHGNS_001764"
     CDS             15313..15813
                     /locus_tag="ACHGNS_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN6937706.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            15825..16319
                     /locus_tag="ACHGNS_001765"
     CDS             15825..16319
                     /locus_tag="ACHGNS_001765"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN6937707.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            16340..17149
                     /locus_tag="ACHGNS_001766"
     CDS             16340..17149
                     /locus_tag="ACHGNS_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFN6937708.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            17146..17967
                     /locus_tag="ACHGNS_001767"
     CDS             17146..17967
                     /locus_tag="ACHGNS_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN6937709.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            18018..20117
                     /locus_tag="ACHGNS_001768"
     CDS             18018..20117
                     /locus_tag="ACHGNS_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFN6937710.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            20114..21232
                     /locus_tag="ACHGNS_001769"
     CDS             20114..21232
                     /locus_tag="ACHGNS_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFN6937711.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            21271..22149
                     /locus_tag="ACHGNS_001770"
     CDS             21271..22149
                     /locus_tag="ACHGNS_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFN6937712.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            22295..22492
                     /locus_tag="ACHGNS_001771"
     CDS             22295..22492
                     /locus_tag="ACHGNS_001771"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFN6937713.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
BASE COUNT         7033 a         3913 c         4847 g         6841 t
ORIGIN      
        1 atgttttatc gtttgatttt tttaggaata aaattcttga tgaacgtaaa tttttggtaa
       61 tcgtttcgac tatagaggta accaaataca ctgaaaacaa ctgctccaat aaaattaaca
      121 aataaatcct tcatagtatc catgattccc acgtctaagt aaccattttg gatgattgtt
      181 ttatctgttt taccatctgc attttgactt tcaatgattg tccgagtgat atctggaaca
      241 tgatagacag tgtttccgtc tttacttaac tccacactat tgatttgctg gatgatcgta
      301 tctttttgca tatcgaattg cagtaattta tctgctgaaa attccacaaa ctcccacata
      361 acaccaactg tcatcgaaaa acagaaggaa acaactgcta gtaataaagg agtcaagttc
      421 atggtttttg tatttttatt gagcaaataa acaagggaaa agccgacacc cccagctaaa
      481 aatccgctga atgcatgtag aaaagtatcc cacattgaaa aatagccata aaaatcactt
      541 aactctccta aaactgttga actaaaaata aaagataaca aaatgatttc tagcatattt
      601 ggaatctgta tctttaatat cttttccaga aaataaggga tttcaaataa aattaatgtc
      661 agtaataaaa gcattgtatt tccccagtgt tggttccaga cctgatccac tagcataata
      721 acgacgatga tacgtaataa aatatagatt ccgtttgtga ttctacctgc agtttttgta
      781 ctgttcgtaa aaaaggtttt caaatgattc ataaaataac tccttcatgt atatccttaa
      841 ttatactaag aagcgtgtgt ttaccaaagc tatatgactt aaagaaaaac agagttgata
      901 ttgtgaaaaa aatcacttaa tatagtataa ggagaaaagg gggatacaaa tgaaaaaatt
      961 agttgctggt ttattaacag ccgttgcact tcttgctatg agcgcatgcg gcgcagatca
     1021 gaaaaataca gcgcagacaa gttctagtac gaaggaatct gccgaagtat catcaggcgc
     1081 ttctgaacaa gtatatacag atccaaaaga aatgaaagaa tcttatgatg tcgtgattat
     1141 tggagctggt ggtgcaggga tggcagcagc tatcgaagca aaagatgctg ggttgaatcc
     1201 agtaattttg gaaaagatgc ctgttgccgg aggaaacaca tctaaatcat caagcggtat
     1261 gaatgcttca gaaacaaaat ttcaaaaaga agaaggaatc aaagactcga atgatgcctt
     1321 ctatgaagaa acgctaaaag gtggtggagg aacgaatgat aaagaaatgc ttcgatactt
     1381 tgtcgatcac tctgctgatg cggtcgattg gctagatcaa aacggtatca aactggacaa
     1441 cttaacgatc actggaggga tgagtgaaaa gaggacacat cgtccgtctg atggctcggc
     1501 tattggtgga tacttagtgg aggggttatt gaataacgtc catgagcggg aaatccctat
     1561 ttttgtcaac gcagatgtga cagacattaa gaaaaatggg gaaacggtca atgaagtgac
     1621 tgtacatgtt caaggagaag aaccaaaaga ggtcaccgga aaagcaatcg tagttaccac
     1681 aggcggtttt ggtgccagca aagaatttat tgaaaaatat cgtcccgatt tagctgatta
     1741 tattacgaca aatcaagaag gatcaactgg agatggtatc accatgattg aaaaattagg
     1801 tggacaaacc gttgatatgg ataaaataca aattcatcca actgtgcagc aggatgaggg
     1861 ggtcttgatt ggtgaggttg ttcgcggaga aggagcaatc ttggtcgatc aagaggggaa
     1921 aagatttgtc aatgaaatgg atacaaggga taaagtatct gcagcaatta cagcattgcc
     1981 tgaaaaatca gcttatttga tttttgacca aggcgtgcgt gatcgtgcca cagctattga
     2041 attttatgac caaaaaggat atgtcacaac tggaaagaca atggaagaat tagcagaaaa
     2101 aatcgattta ccaaaagaaa cactagcaaa aacagttgat ggatggaata cggatgttgc
     2161 ggagaaaaaa gatgctcaat tcaaccgtgc cacagccatg gaacatgact tatcgtctcc
     2221 gaattattat gcgatcaaaa tcgcaccagg aatccattac acgatgggcg gtgtgaaaat
     2281 caatacgaac actgaagtat tagataaaga aaaccagcca attagggggt tatatgctgc
     2341 tggtgaagtc acaggtggct tacatggaaa caaccggatc ggtggaaatt cagttgcgga
     2401 gatcattatt tttgggcgac aagcgggcat acaagcagct cgatttgctt cggaaaatga
     2461 atgaaataga acgtaaataa aaagaggttg tgaaatcagc gttttgagct aataccgagg
     2521 gtcagctgat tgaacactgt ttagtgaaga ggttgtgaaa aagctgccct gcatcaagta
     2581 ataagaacag ccaagtaaaa acagcttctt atgtttttaa cttggttgga cttattaccg
     2641 cagatgcaag cttttgaaca ccgtttattc ggaaaaagag cgtgggacaa aagtaaagaa
     2701 cacttttgtc tcacgctcta aacacgtata aacggcggaa gcagaagaaa ttccttcgga
     2761 aataagctga aattcacaaa aatttgaaaa gcaattttcg tgaatttctt cttatttctt
     2821 ggagttaaac gcttctgtcc cagcctcttt tttgatcagc atgattttat tcagttatct
     2881 tcaaaatgat ttttccgatg tttttgtttg cttccatata ttcatgcgct tgtttgactg
     2941 cgctgaattg gaatgtacgg tcaatgattg gccgaatctt gccattttct agaaaaggcc
     3001 cagctttttg cataaaagtt tgtgcgagct ctgctttata ctgatcactt ctaggtgtca
     3061 ataatgtacc aagcagttgg acacgtttcc caatcaatac accaaggtct atctcaggca
     3121 cgatggtccc acctagcata ccaatcaaga cccaacgtcc atcaacggct atagaagcta
     3181 aattcttttc ccagtaagaa gcgccgatga aatccaaaat aacatcgact ccccgacctt
     3241 cagtgatttt tcgtacctct tcttgaaagt cttgtttttt atagttgatc aattcatctg
     3301 caccaagttc tttacaaaag gcgagttttt cttctgatcc tgcagtaacg atgattttcg
     3361 cgtgagaaag ctgtctagct aattggatcg ctgctgttcc cacaccactt gctcctgcat
     3421 ggatcaagac agtttcgcct tcttgtaatt ttccaagcca gtaaagtgtt tgataagcag
     3481 tcaaaaaaac ttctgagatt ccagcagcag aaacatagtc tagcgtatca gggagaagga
     3541 ttgcacgatc ggcaggcatc acagcatatt cggcataacc acctagattg actaatccgt
     3601 atacttttgt cccaggagta aaagtggaga tatcactttt attctcaact acctctccag
     3661 ctacttcgac tcccaaaata gggtagggag gtttcaaaga gactgattct cttttcataa
     3721 tatctgttcg attgacagct gctgtatgga ctttgatcag taattcgcct tgttttggtt
     3781 taggaacagg cacttcaatt tgttctaata catctacaga accaggatgc ttgattgaaa
     3841 tggcacgcat ggatctattc ctcctttgcc cattcttgga atgctgtatt caactctttg
     3901 ctaccttcag aaagtactag agtcgtacga gaaatatccg tttcttccac tagagcggca
     3961 agtacagcag caactgtaga gcgagtgatc gtttttgatg ccttctcatt aggcttagtt
     4021 aattgaatgg aagtaacttc ttcatcgtta gtcaatgtga ctggttgaag aatgacaaaa
     4081 tctaagttag ttcgtttcag ccattcatct gcataatgtt tagtaatata ataatctgtc
     4141 atggactcag gccattttgc tggttcatct gcataaacag cactgaccat cagatagcga
     4201 gagacatgcg cattttcagc tgcgatcact gttttgattg cgccatccaa gtctacttgc
     4261 agaagattct ttccttgaga gccggcagca aaaatcaata catcgatgcc tttgaatgct
     4321 tcggtcattt tttccacgga ccaagttaaa tcaaaagaga catacgaaac attctctttt
     4381 tttactaatg tttgggaagc aacatctctc acccctgcaa aaatttcatg ggaacttgga
     4441 gctaaatctt ttatcaggtg tcggccaatt tgtccgtttg cacctacaac aaaaattttc
     4501 atttggatca tcctttcgac gtcaaatctt tccattcaat agtaacacca aaagagaaaa
     4561 aaatcagaga tcggctacga tgactatcag ttagtaagag aagggtgctt gaagtatctt
     4621 aacaagacat tgcatactgc tgcagtcgca agacctgcga cgattccttc aggtatacca
     4681 cttgtaaaaa taaccgctag aatagctgaa taaacagcat ttaaatttgt tccgatgact
     4741 ttggcataat cttgctggaa taaaaagtag atcatattca tcactaaaat cgtattggtc
     4801 aatgaaccgg aaagtccagc taagaataaa gaaacggaat ctgctttttg tttcattagt
     4861 ttgcgcagac ctttataaac gaaataagga accaccccaa tcaagatgcg aggaataaac
     4921 gcgatcagca aggctttcca gcttccatgt tctgttccga tgactgggat aaacggagaa
     4981 aagacaaaag acaaaggtgt ttggataact gtgctacgga tcatgctgat cagtccaaat
     5041 gtaccgccaa gaaaagcacc tagtcttggt cctaaaacga tcgaagcaat gattacagga
     5101 atatgcatgg ttgttgcatt gattggaccg atagggataa aacctaaagg cgtaacagct
     5161 aataaaatca aaatagctaa aaacatcgca gttaatgtaa agttttttgt atttttcata
     5221 agtactcctt ttttctaagc atttctttca ttaagagtca gcatgacctg ttcgatgatc
     5281 gtctgaatat cagctaaggc accttttcca aaatctccac aagctaaaag agattctctt
     5341 ggttcaattt cctgatagcc gacagatctc aatgtttcca gattacgctg agtgatcggg
     5401 ttttgataca tattcgtgtt catggcaggg gcaatcaatt ttggtgttcc cactggaagc
     5461 gccattgcga ctgttgaaag aagatcgtct gcaatgccgt ttgctagctt gccgataatg
     5521 tttgctgtag caggcgcgat gagaaacaag tcagcttgtt ttgctagttc gatatgattg
     5581 attacctgcg gatttgattc ttgcatgacg tctgtatgga ctgcgcgctt cgacagagat
     5641 tgcaaggtta atggcgtgat aaaatgtgtg ctgcttttag acataatcac atctacctgg
     5701 gcacctaatt tgacgaattg acttgtaaga tcagcgcttt tgtaagcaga aatacttcca
     5761 gtaacgccta gtaagatctt tttcattttg attcctccag tctaattttc tcagtaatca
     5821 atgcagcaat ctcagattta ctttgagctt cttccactgt tccatcttta gataagagat
     5881 agccgtggtg tgtctcgcca tggatttccg tcaaatcatt tgcgaaaata aaatcagttt
     5941 tgttatttat cagactattt tttgcgactg ccagaagaga ttcctttggt acatctacta
     6001 atagtttgaa accaatgatt tttgcttttg gctgccattc acgtagataa gaaatgattt
     6061 ttggtgtttt cttcaaaacg agcacaagat gctcggtgtt agaagagatc tttgaaacag
     6121 tggtatctgt ctgttcattt tccgcaaacc aattgtccag ttcttcttgc gttgaagaag
     6181 tgggtaggtt ttttgccagc tgttcttctg aaaaactaaa agcaggagta aaatcactga
     6241 ctgccatact gtggataatc gcatcgtagt gttctttttt cgttagttct tctaattgta
     6301 aaaataagtc cagtgcagat tcgatcgtat aaatcgttat attggagtgt cttttcggtt
     6361 tcagtgcttg tttcgtagtc acgtaatcga ttgtggtatc cggattggca gctaaataat
     6421 cagctaatgc ttgccctaat cgtcctgtcg aatgatttgt aatagcacgt acttgatcga
     6481 ttttttcagt cgttcctcca gctgtgatta gaatcttcat ttatggtcac ctacatttac
     6541 tagtgttctt ccttggtgtg ttccagcaag tagtttagat gctgtttcag gcaattgatc
     6601 cagagtaatc tcttggacga ccagttcatc tgcaatctgc aaatccgcca tctgttgcca
     6661 taaagaaaga cgttgatcta ttggtacatt gacagaatca attccaatca actggatgtt
     6721 tcgcagaata aaaggcaaga cagtcgcgtt tagtttgatt cccccagcat tgccacataa
     6781 agctactgca ccgttataag agatcaatgg aagaagactg cttagctgct cgccacctac
     6841 tgtgtcgatg acatagtcga tttgctgttt tcctaaagct tttgttgtct ctggcagaaa
     6901 ttcatcaggt cgtacgactt gcaaagcacc tagacttttt agccatgtga cagactcttc
     6961 ttttctggaa aaggcaatga ttgactggaa tcctagttta tgtaacaggg ctatcgctgt
     7021 actgcctaca ccgccagaag caccagtaac gacaactttt ttatcggagg tcataccttg
     7081 acggattaaa gcgttcacac acaacatagc agtaaaccct gctgtaccaa ggatcatcgc
     7141 ttgacgcagc gtcatatttt ttggtagagg aactaaccag tctcctggta ctttttgata
     7201 ttggctataa ccgcctggat gggtgacgcc aagcccaaaa ccagtagcaa gtacaagatc
     7261 ttcttttgaa aaacgtggat tgttactttg gacgacagtt ccggataaat caatccctgg
     7321 agtcatcgga tatgttcgta tcacctgacc agactcgctc attgccaatg catctttata
     7381 attgacatca gaataggcaa tttttaccaa gacttcattt tcagccaact cagtaaatgg
     7441 cagatcactg attgctgtat ggaaaacagg ttcctttatt acctgaaaag atcgaaaagc
     7501 gttcattcgt gagacactcc ttttaaaatt ttcttttatc ataccataaa gcctactctt
     7561 ttattcttgt tcttccgtaa atttagacaa aaattaggtg aaaaataaag aatcaaacaa
     7621 ctagaattta ttccaaaaga taaagaaata ttcagcaaaa aaaattttac aaagggttgc
     7681 ctttttcaat aaatttcgta tactaatacg agtggtagta aacaggaagt ttactattag
     7741 aaaacttcga ttcggaggaa attatggaaa ttggtgagaa gttaagaaat ttgcgcatcc
     7801 aaaagaattt gacacaagaa gaactaggcg aacgcacaga tttgtcaaaa gggtatattt
     7861 cccagttgga acgagattta agttcaccat caatggaaac attcttttct attcttgaag
     7921 tcttaggtgt gactccagaa gaattttttc atcaagaagc agcgaatctg caagtggttt
     7981 attcaaaaga agatcatacg gtttatttcg atgaagaaaa cgggtatgaa ttggaatggc
     8041 tcgtggctga ttcgaatgaa aaagaaatgg agcctgtctt gctgactttt gaaggtgcag
     8101 gagaatataa gacgtttgaa ccttcattat ctgaaacgtt tatttttgta ttagaagggg
     8161 aattagagtt aagcttagga gagacggtct atgtggcaaa agcaggacag tcgatctatt
     8221 atcaagcgac aaaacaacat caattaagga atcatctcaa aaaacgaaca aaagtattga
     8281 ttgttgcaac gcaatcatat ttgtaatata gggggtaaaa acgtggggaa accaatcatt
     8341 tcatttgatc aagtagtgaa acgctatgat gatgaaacag tattaaaaaa agtaagtttt
     8401 gagatcgaac aaggaaaatt ttatacactg cttggtcctt ccggctgtgg taaaacaacg
     8461 atcctacgta ttatagctgg attcaccgaa gctacagaag gggacatcta ttttgaaggg
     8521 aaacggttga atgatttgcc agctaataag cgacaagtaa atacagtctt ccaagattat
     8581 gcactttttc ctcatatgaa tgtttttgat aacgttgctt ttggattgaa gatcaagaaa
     8641 atgtccaaat cagatatcga aaagaaggta aaagatgctt tgcgtatggt ccagcttcct
     8701 ggatacgaaa aacgggagat tagtgaaatg tctggtggtc aaagacagcg tgtagcgatt
     8761 gcacgagcaa ttgtaaatga accgaaagta ttgctattag atgaaccgtt atcggcgctt
     8821 gatttgaaac tacgtacaga tatgcagtat gaactgcggg atctgcaaca acgattagga
     8881 atcacgttta tttttgttac tcatgaccaa gaagaagcgt tggcaatgag tgatgagatt
     8941 tttgtcatga ataaagggaa aatcgtccaa agtggtacac ccgtagatat ttatgatgag
     9001 ccaatcaatc atttcgttgc tgattttgtt ggggagagca atatcgtgga cggtgtaatg
     9061 atcgaagata atctagttga gtttgttggt aagcaatttg aatgtgtcga tggcgggatg
     9121 cgtctaaatg aaccagttga agttgtttta cgcccagagg atttgacgat cacgacacca
     9181 gataaaggaa aactagtcgt aacggtcgat actcagctgt ttcgtggtgt ccattatgaa
     9241 atcatctgtt atgatgaaca gcaaaatgaa tggatggtcc attcaacgaa aaaagctaaa
     9301 gaaggaagca aagtaggatt ggcctttgag ccagaagata tccatgtcat gcgtttcaac
     9361 gagtctgaag aagaattcga tgcacgtctt gacagctacg aagaataggg gggacaagaa
     9421 tgaaaacaat gcgcaggatc tattccgtcc cttatatttt gtggctcggt ttattcgtga
     9481 tcgcgcctgt attaatgatc atttatcaat ctttttttga tatgaatggg caattcacgc
     9541 tgaataatta tctatcttat tttacgtcgg ggacatcgct ttctatgacg atcaattctg
     9601 tatggtatgc atttttgatc acgtttttca cgctgatcat cagttacccg actgcttatt
     9661 ttttaacaaa actgaagcac aagcaattat ggctgatgct gattattttg cctacatggg
     9721 tgaatctgct gcttaaagct tacgcattta ttgggatttt cagtgtgcac ggcagcgtga
     9781 atcatttctt tgattttatc gggatcgggc cgaaacagat attattcaca gacttcagtt
     9841 ttctgttcgt ggcaacgtat atcgagattc catttatgat tatgccaatt ttcaatgcat
     9901 tggaagagat gaatccttct ttgatcagtg ctagtagaga tttaggcgcg aatagctttg
     9961 agaccttccg acgagtgatt tttccgttat cattaaatgg tgtgaaaagt ggtgtccaag
    10021 cagtatttat tccttctctt tctttattca tgctgactcg tctgattggc ggaaatcggg
    10081 tgatcacttt aggaacggct atcgaacagc atttcttggt tacacaaaac tgggggatgg
    10141 gttctacaat cggtgtgatt ttaatcattg ctatgttcat cgtgatgctc ctgacaggcg
    10201 agaagaaaaa aggaggtcgc tcataaatga aaaaatttaa atggtcgtat ctttatttag
    10261 ggatcgtctt tttattccta tatgcaccga tcttctatct gattttttat tccttcaatg
    10321 aaggaggaac gatgaacagc tttacaggat ttacttggga aaattatgaa gctgtttttg
    10381 aagatacacg tttgatcatt atcgtattga atacgttcat gctggctttc ttatctgcat
    10441 tgatcgccac gatcatcggg acatttgggg ctatgggaat ctattacacg aaaaaacgac
    10501 gtgcacggac agcgttgttg agcatgaata acatcttatt agtctctcct gatgttatta
    10561 tcggtgctag tttcttgatt ttctttacac tactaggatt cggattaggt tttcagagtg
    10621 tattgctttc ccatattgct ttcagcattc caatcgtcgt cttgatggtc ttgccaaaat
    10681 tgcaggaaat gaacgattca atggtcgatg cggctagaga ccttggtgct aataatttcc
    10741 aagtagtcaa aaatatcatt ttgccttatc tttctccagg aatcatagct ggatatttta
    10801 tggcatttac ctattctctt gatgattttg cggttacatt tttcgtcaca ggaaatggct
    10861 ttacgacgct ctcagtagag atctattctc gtgcaagaca aggaatcagt ttagaaatca
    10921 atgcgctaag tgcgttagtt ttcttgttct ccatgatatt ggtcattgga tactacttta
    10981 ttagtaaaga aaaccgtcca aaacggctga agaaaaacag aagagtacgc gagggggtgg
    11041 cacggctgcg atgaaaaaat tacaatctct gtttttaggt attctcatca ttattgcggt
    11101 gctgctgctt ggtgttcgtc aacttgaaaa aacaagtggc atgagtggag caaaggtact
    11161 taccatctat aactggggag attatatcga tcctagtttg atcacgaaat ttgaaaaaga
    11221 atatggatac aaagtgaatt atgaaacatt tgattctaat gaagcgatgt tcacaaaaat
    11281 ccagcaaggt gggacaaact acgacatcgc tataccaagt gaatatatga tccaaaaaat
    11341 gatcaaagaa aaactagtac ttccattaga tcattcgaag atcaaaggat taaataatat
    11401 tgatccacga tttttagact tggattttga tccagataat cagtattcga ttccatattt
    11461 ttggggaact ttagggatcg tatataacga taaattcttt gatgcaaatc aaattaagca
    11521 ttgggatgat ttgtggaagc cggaattaaa agacagtttg atgctgattg acggtgcacg
    11581 agaagttatg gggctttctt tgaatagttt aggctattct ttgaatagta agaatatggc
    11641 acagctgaca gaagcagcaa ataaattgaa tcatttaaca ccaaatgtca aagcaatcgt
    11701 ggcagatgag atcaaaatgt atatgatcaa tgaagaagcc tccgtggcag tgacattttc
    11761 tggcgaagct gccgatatga tgtgggaaaa tgaacattta cattatgtga tcccaccaga
    11821 aggatccaat ctttggtttg ataatatcgt tatgccaaaa accgtgaaaa ataaagaagg
    11881 tgcctatgat ttcatcaact ttatgctaga accggaaaat gcggcacaaa atgcagagta
    11941 tattggttat tctacaccaa atgaagaagc aaaagcgtta ttgccaaaag acatttcgtc
    12001 ggacgaacaa ttctatccat ctgatgacac gatctcccat cttgaagtct acaaagatct
    12061 aggatcgaaa tacctaggca tctataatga cctattctta gaattcaaga tgtataggag
    12121 ataatggcaa aactcagtaa gaatggggac ttatcaacgt tctaaagagc taaaaatcgt
    12181 cctttttcta tctgagtttt ccaatcttct accaaaagcg gatgaaacgc cgcttttttt
    12241 gtgttatttt attttttcta ccacgttttt ctaccagaga aaagtggaca aaaccattgt
    12301 aaaatagggc ccgagacggg ctagtaaatc taagattgaa aaagttacag aaggataaag
    12361 taattggaaa ctatgcaaat atacttaacc cttctgtaat ctattaataa aactaatata
    12421 tgaatccagt tcaatatttg tagctatgaa aaaacataaa aatcatatta gataaaaact
    12481 aaattggtag ataaattatt ttgtcagata tctgaggcaa atattcgact atttgtgttt
    12541 caaagtaaaa attggaatac aaatatagaa tagacatatt gcagccgata gtttattatg
    12601 cttttagaca cattaaactg gaggaaaaaa tgaaaaagag cgctaaagat cgagtttttg
    12661 atgagctgat acgactaaca gggaatatgt cttctgtcac tgccgagaaa atttcgaaga
    12721 atctaggtat ctctcgtcag aatgccagcc attatttgac gagattagtg gaagataaaa
    12781 aggtcgagaa attaccggga aagcctgttt tgtggaagcc tttagatgaa tatgcggttg
    12841 tagataatac ggagcaaata aacgaagcct ttcattctgt agttggtcat gacggaagtc
    12901 tgagagaggt cattcaaaaa tgtatagccg ctgtcaaata tccgcctaac ggactcagtg
    12961 tattaataaa tggagccacg ggtgttggaa aaagtttttt agccacaaaa atttttgaat
    13021 acgctatcca tgagcaaatt attgaaaaag atgcaccatt tgctatctta aactgtgccg
    13081 attatgcgga caatcccgaa ctgctttcgg caacactgtt tggatacaag aagggggcct
    13141 ttacgggggc tgaaaaggat accgatggtt tattggcaac ggccaacaac ggctatcttt
    13201 ttctcgatga ggtccatcgc ctatcgaaag aaaatcaaga aaagctcttt ctatttattg
    13261 atactggaaa ctatcgtcca gtcggtgaaa atgtaaattg gcattctgct aaggttcgtt
    13321 ttattttcgc cacgacggaa aaaggtgaaa attatttatt agacaccttt gatcggcgta
    13381 ttcaaattag tgttatgttg ccaacttttg aagatcgccc cattcgagaa cgattagagc
    13441 tgattcaatt attttttcaa aatgaagcgg atgttttaca aaaagatatt attgtttcaa
    13501 gagaagcctt gtctttaatg ttatctcatc cctttagtgg gaatattggt aagctgaaga
    13561 atatcattaa aatcagttgt gcggatgtgt atagtcgaac acaagaagaa ccattaacga
    13621 ttgggaaaaa tgagatcttg attcagttaa atatgctgga atccgaagta gaaagcttgc
    13681 cgattgctag tttattgata aagaaaaatc aaaagataaa aacggatcga atcaaaccgt
    13741 ttgattatga ttcgattttg gaaaaaatta ctcagctgat aaatgaaggt aatttcaatg
    13801 aaaatttttc ttcgataaag aatgaagtac agcatttggt gcatcacatt gataaggatt
    13861 cttttttatc aatgagtaga aaattgatga ttcagatgtt tagaaaggtc tgggtatccg
    13921 ttttaggaaa aacgtacgga ttgacgacga gtggacctgt tgcagaaatt attagcagat
    13981 cctatgtcat ttccccaagc tataagacag acttaaccgg gataaatcgt acattagctg
    14041 caaatctacc tcggacgact tacttatgta atcagttctt ggagcattta ccaccgctct
    14101 cagaaaagga cgagaacttt ctaagactgc ttattgtcgt gactttatct gattatgtag
    14161 atgaatccat cgagctgaag ggcctgttgc ttgctcacgg agatagtacg gctagcagca
    14221 ttcaagcagt agtgaatcaa ttatgcggca attatgtttt tgaagcgttg gatatgccag
    14281 tagagagtaa tgtgtcagag attgtcgaat cgacaaggaa tttcgtgaaa cggcaagtga
    14341 atacagatag tctagtgctg atcgtagata tgggctcttt aaatcaaatt tacacacaga
    14401 tcaagaacga tttaaaaggg gagttacttc ttctgaataa tttgacgact tccattgcac
    14461 ttgatattgg cttgaagatg acgatgaatg cgccatttaa atagattgcg gaggatgcag
    14521 cagacaagta ttcaatcaat gttcaatact ttgaaggctt ttctcaaagt agcaacatta
    14581 tcatttcctg tatgtcgggt ttggggattt cggacaaatt gaaagatgtt tttcgtcatc
    14641 atttatcgga tgaatccatc gaagttttca cgaaggacta cagagaacta cgtgaattaa
    14701 tcgaccaaaa tgatgaaact tattttgaca agacaaaatt ggtcatcacg acttcggatc
    14761 ttccacgttc cttttcaatt cctaatatta atgtttatga catcttagac ggtgatggag
    14821 cagccaattt ggagcatatt ttgagtcctg aaatgaagcc ggcagaattt aaccgcttga
    14881 ttcaggagct agtaaaattc ttttcgttgg aaggaattgc ggaccgtttg aactttctga
    14941 acccaacgat cgtgatgagt gaagtggaaa cagtcatttc taaatatgaa aattactatc
    15001 atttcacttt aaatggccgt gtaaaactaa atctctatat gcatacggca ttgatgatgg
    15061 aacgtttatt cttggctcgg acagaaaaat ctgaagaacc gaattatgat ttgaaaccag
    15121 atgaagatga attttattat gtttctagaa gtatttttca atcaatggag atgaaatata
    15181 attttaaagt caatgcttat gagctttcgc tgctctatga attattagga ccgtatattc
    15241 aaaaataaag tgtctaaaaa agtgtctaat ctgttttttt taggttagac acttttttta
    15301 atgtgaatta acgtgattaa accaatgttt ttaaaagttg gcacaggaat tgcttatata
    15361 aaagtgaaca gaaagaaagg aattaaccat atgaagaaaa ttctaatagc gactcacggt
    15421 cacttagctt caggctttct tagcagtatt caattactga cggggaaggt tcaagagatt
    15481 acggtgatta atgcttatgt ggatgagtgt gattttgaac aggagttgaa tgtatttgta
    15541 aaccaggtga acgagcagga tcagattttt gtttttaccg atttatttgg cggaagtgtc
    15601 aatcagaaga ttaccaaaac ctttttggag aaagagattg ctgtaactat tttgtctggc
    15661 tttaatctgc caattctgct cgaaatagta ttggcaacca aggaattaac aaaggaggat
    15721 attcaggaac tggtaaataa atgccaacag gaattaaaga tgaccattct tgatgaggta
    15781 catgtagtaa atgaagagga agattttttt taggaggaag aaaaatgatt aaagcagtgc
    15841 gaattgatga acgattaatt catggacaag tagcgatgac ttggacaaag agtctaaatt
    15901 taaccgggtt agtagtagca agcgatgagg cggctaccaa cgatattcaa aagatgacat
    15961 tgaagatggc tgcaccatcg aatgtcaaag tcattattaa aacagttgat gacgcgattc
    16021 atttgttaaa agatccgcgg gcgaaggaca tgcgcttgat ggttttagtc cctaatgtca
    16081 aagatgcggt aaaagttgcg aaagctttcc caacggaaat cgaattaatg aacgtgggga
    16141 atgctgggaa aatgacaccg ccatcggaag ataagaaaac cttatcaaag gaagtgatgt
    16201 taacgacgtc agaaattcaa gacttaaaag aattagttga actttatcca gataccgttt
    16261 ttcagcctac accggcgatg gaaaaacgtt cagctatctc tattttgagt gatttttaga
    16321 aaaaggaagg agaaaaatta tgtatgatgc atttgtagtc gctttggccg tttttattgg
    16381 agttgctgga catgaaatgt tcgggatgtc catgctgagt cgtccgattg ttgttgctcc
    16441 gttagcagga gcgttgatgg gggatttgca gacggggttg attgtcgggg cgtcactaga
    16501 agccatcttt atgggtgtcg tcaatatcgg aatctctagt acggcggagc cttctttggc
    16561 tgctgggctg gctacagcgt tcgcaatcaa aatgggagga aacgtggatg cgattattcc
    16621 cattgtcttt ccactagcag tattaggatt acagttaatg aatatgattt tttcttttgt
    16681 atgtggaccg atggctgcta aatttttcaa atttgcaaaa tctggtgatc aaaaaggaat
    16741 catcaatctg cacttcttta tgtggtttgt ccactatgcg ctttatgctc ttattccatt
    16801 ctttgccgtt ttatttggag cagatgttgt caaaagcgtc ttggataata ttccagaggt
    16861 catcatgaac ggattaacag tcgctggaaa tctgctgccg gcagttggga tggcgatgct
    16921 gttacggatg ctttggagta aaaatattgc tgtgtggttt ttctttgggg ccgttgttat
    16981 ggcgtatctc gagttaccat tgattgcaat tgccgcagtt ggaacgatta ttgcaattgt
    17041 tatagcccaa agagatctac aactaaaaaa tatatctgtt catgcagttc aaagcgatgc
    17101 tgctgtaaat gcaacgcttg accaagaaga ggaggatttc tttcaatgaa attagctgat
    17161 ggaatttcta aagatgagaa aaaattggtt cggaagatgt tttggcgctc gatgactatg
    17221 tatgcttcgg tgaatccggt gacgatgggc ggcggtggtt tcgcctattc tatgatgccc
    17281 ttcattaatc atttctacaa aagtgaagaa gatcgagctg cggcgctgga acgtcataca
    17341 cagtattttt cgacgaccat tccttgcgcg tcctttgtca tggggattgc ggcatcgatg
    17401 gagaaggaaa attctgaaaa agctggtttt gacgtggaaa gtatcaacgc gatcaaaacg
    17461 agtttgatgg gaccattggc tggaattggc gattcattgt tctggggagt ttggcgtgtc
    17521 gttaccgcgg gactagcgat caacttagcc aatgccggaa atattttggc accaattatc
    17581 tttttagtta tgttcaatat acctaattac ttgatgcgat actatggcgg cgttttaggc
    17641 tattcattag gctctaaata tattgaaaag ctgtatgcga acgggttaat tgaaattctg
    17701 acaaaagctg ctagtatctt gggtctgatc atggtaggtg caatgacgat cagtatggtc
    17761 aaattcaata ccgttttgag cttttcaatg aagggcgaag aactaatgaa tgtccaagaa
    17821 atcctcgata gtatttttaa aggaattgta ccgttattgg taacattgag ctgtttcaaa
    17881 ttactagata aaaaagtcgg cgtcatcaaa attatttttg gaatggtcgt cttgggaatt
    17941 ttattgtcat tacttggaat cgcatagtag gaagctgcag ctactaggct gcagtttttt
    18001 tagcaaggag ggaaaagatg actattaatg aagtaattta ttcacccaaa cacccatttt
    18061 ttaatgctga gttaaacgac ggaaatgtga ctacttcaaa cgaatacagc tactatcgga
    18121 atattcctga tgtaaaggga ttgaccttcg atgtaagtaa tcaaaagaca ttagcaaacc
    18181 ttgatgtttg cgggacgatt caaacgttga ccttttatcg agaaaattta ctaactgagg
    18241 aaaaaccagg tgtttgggtc aataaacagc tctctcaaac caattcgtta tccttaaagg
    18301 ttgaagtaaa cgggattgtc taccatttgt cagaatcgga tcatcgtatt gaagtcgatg
    18361 tgattcaaga ctgtttgcct agatatattc atcattattc gacgtttaag gtcattgtag
    18421 tatcctttgc tcccatactg gctaataagc gtttaagtat gcttgttcaa caggtttaca
    18481 ttgtgaatga aacacacgaa agccttcaag tttcagtgaa agatgtgcct tcgtatcaag
    18541 agaagtattc ggatcagcaa aatgtgttga tttctcaaaa aggaaataag gacacgatcg
    18601 cacaacagga agtggcaagc ttcgccattg cttttgtgga cccaaatgct tttgaagaag
    18661 taatgacttt tcaggaatct gatatcgaaa aatggctgaa ggaaacattg aactattttg
    18721 ctcagctatt tgggcagctg tcaatgccaa acgatagagt tgtccatctc tatcgtcgtg
    18781 cactttatca aagcttttct agtttcggta tgaatcgaca gggacaggtc gtcggcagca
    18841 attggggcag ctatcctgct actaatcgaa tttggaataa ggatatgtat tacgcatcat
    18901 tgccattcac catgtttgaa ccggagttat gtcaaaaaac ggttctatgg tttgatcaat
    18961 atggcgttaa gtttcctggt tcaaaattta caggaggtat caatcattct ctgagtaatt
    19021 cgttatccag tgcgttactg gctagtcttt attttgaaca tactggggat acaaaattct
    19081 ttgagcggtg tccgcaggtt ctactgaatg cttcaagaat tattgaggac atattagctc
    19141 aacgtcggtc aggggaacca atgctttttc catctgtttg gttatcagac gcctttgcac
    19201 ttggaaagta tcataccggc agcaatcttt gtctttggaa ggcctgtatt ggacttgcag
    19261 aaatttttga agccctagaa caactgagtc ttagcaagcg atacaaaaac attgcctgta
    19321 aactaaagga atcaatttgt aaacaaatga ctatcgatgg tccttttggt gagcaatttt
    19381 tggagggaat cggtgatgag gagaaaacaa cctattcagt gaagcactat caaaaaccga
    19441 ttcttgagca agggttaatc tttttaagtg atgtgattgt ggatggaaaa attaatttat
    19501 tgatgcatga tggagaagaa tcagacacta cgttgatgcc tttttatcaa tttttagata
    19561 ataaagatca tttatatcag aacaccatgg ccttttctgc tagttctttc aatccaacct
    19621 atagcgagga aatcaaaggc atcacttggg gattagaatc cggcgccact tttcctggct
    19681 ttatcactgt tttaatgagc gagctgcaca accctcaagc tttcgtaacc cgattagaag
    19741 aactaatttg tttggcagat ttagacggtt cgtggtggtg gtggccgtat cgcttagagg
    19801 ctcaacaagg agaagtcgtg cgagactttg gctgcggcaa atgcggctgg gcatcgggtc
    19861 tttttgtcag cctaatgatc agtcaatatt tcggattgaa attcaaaaaa ggagtattgc
    19921 aaattgatcc tgttgataat ttaacgtttt cttggaagaa tttgaagttc ggtcaagcct
    19981 tcatttcgat tgaatgtacg caatccgagt tgagtatttc aaatttgtca gaaaagccat
    20041 tgaaaataag tgatacgaaa aaaattcttc acgtagaaaa gggaaaaacg attcacattc
    20101 aaaggagaaa aagatgagta tactaaaaaa tattacccaa tttaaaaaaa tccagagtcg
    20161 agcaatcact cccgaaaatc caacaggcga gaaaggtaaa gctgcgatgt tgccaagcga
    20221 gctaggacca agccgcaaag gtcgtggcgc aattccgctg acttctggtg aagaaactgt
    20281 gattgcggat attactggaa cgggaataat tcgtcatatg tggatgacga ttcgtgatcg
    20341 aacagaattt ggcagttttg ttttacgtga tttaatctta agaatttatt gggatgggtc
    20401 ggatagccct gctgttgaaa gtcctttagg ggatttcttt tgtaatgggt ttggagagcg
    20461 ttgcgaggtg aactcgttgc cgatagtggt taatcctaca gggggcttta attcctattt
    20521 cgaaatgcct ttccaaacat ccgctaaaat tacgatcgag aatttacatc cgaaggatat
    20581 gtcctcattt ttttatacga taaattacgc tttggtggac gaattggaag aaaatagcct
    20641 atattttcac gcaaattggc atcgggaacg cattaccaaa ttacaggagg attataagtt
    20701 aattgatcga ttaaagggtc aaggctatta cgtgggtact taccttgcgc ttacctgcct
    20761 agaacgttat tggtggggcg aaggcgagtt taaattctac attgatgggg atagtgagta
    20821 tccgaccgta acctcaacag gttcagaaga ttattttggc ggggcgtggg cctttcatga
    20881 gtacgatgag ttcggacgtc cacatgcgaa aaactattca acagcctttt tagggtatcc
    20941 ttaccaatca aatagagatg ccacaagaga tcgttttgaa acgggaaaat tgaatgccgt
    21001 tcatgccttt ggtgacgacg caatgccacg acatggtctt tatcgttggc atcttcaaga
    21061 ccccatctgt tttaccgaag atttatcggt cactttacaa caaatcggca acgatgatgt
    21121 tcgcttattt gagcggcaag acgatgttgc tagtgtcgct tactggtatt caacagaagc
    21181 ttcaggaaat actaaacgtc atttcactga tcgagaacgc ttgccgcgtt aatgaagtgt
    21241 ttttcacata ttcgaaggga ggaacctatg atgatatatg gggcaattga agctggaggg
    21301 actaaatttg tctgtgcagt cggtactgag gaattaacga ttttggaaag agtcagtttt
    21361 cccacaacaa ctccagttga aacaatgaag caggtcattg atttttttaa accttttaag
    21421 gaacaattgg caggcatcgg ggtcggctgc tttggtccaa ttgatattca atgcaaatca
    21481 acgagtttcg gtcatatcac atcaacacca aaacttgctt ggcaaaattt caattttgta
    21541 ggaacgctaa agcaatattt caatattccg attgaatgga caacagatgt caatgcggct
    21601 tgctatggtg aatatgttgc tggttctggc aaagggttag caggtgttgc atacttcact
    21661 gtgggaacag gtatcggagg tggtgccttg ttcaatggga ctttcgtgga agggttcagt
    21721 catccggaaa tggggcatat gttgatcaag cgacatccaa aggacaattt ttcaggaaac
    21781 tgtcccttcc accaagattg tttagagggg atggcagcag gaccagcgat tgaaaaacgg
    21841 ttgggtgtaa aaggccagaa tttattggct gacgattcgt tttggcagat tgaagccttt
    21901 taccttgccc aatgtgcata caatacaaca ctgatgtttt cgccagatag aattattttt
    21961 ggcggtggtg taatgaaaca agaacacatg aagaaaaaag ttcaggataa atttgttgag
    22021 cttataaatg gatatgttga aataccacca attgatagct acattattac gcccgaatta
    22081 ggtgataatg ccggaataat cggcggatta gcattggcca gaaaagcggt aagaaataag
    22141 caaccataat taatgaatgt ttcattgata ctcatgtcaa aagtatttaa ccgagtaatc
    22201 aaatgatcaa atgaagttgg ataaagcgaa aatgaattca aataaaaagg ctctaagtca
    22261 aatcgtgttg ttttcattaa atcactgaaa ggaaatgctt ctgcgtttcc ttttttcttt
    22321 ttgttgtgcg ctgtatacct tgaggatatt cactggtggc gtacacttaa atagtaatat
    22381 attgtgttac tttatgtctg tggtaatagt tatagttgct taccaatgta tgttcatttt
    22441 tcgaaatgtt gacttcataa tccttggctt aatgtttgaa tttttttggt gatagatttt
    22501 ttcagccgta taaaataaat cgtaattcta ttatagagag ttgtgattat aaaaagatag
    22561 caagaaaaaa aaagaaagtt tctatacatt ttaaatattt tattcctatt tctattgcta
    22621 aaaaagtaga aatt
//
LOCUS       DAZBTF010000045        22464 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000045 DAZBTF010000000
VERSION     DAZBTF010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.045"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            144..542
                     /locus_tag="ACHGNS_001772"
     CDS             144..542
                     /locus_tag="ACHGNS_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFN6937714.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            526..1220
                     /locus_tag="ACHGNS_001773"
                     /pseudo
     CDS             526..1220
                     /locus_tag="ACHGNS_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            complement(1814..2701)
                     /locus_tag="ACHGNS_001774"
     CDS             complement(1814..2701)
                     /locus_tag="ACHGNS_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFN6937715.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2886..3725
                     /locus_tag="ACHGNS_001775"
     CDS             2886..3725
                     /locus_tag="ACHGNS_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFN6937716.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3921..4895
                     /gene="sgrA"
                     /locus_tag="ACHGNS_001776"
     CDS             3921..4895
                     /gene="sgrA"
                     /locus_tag="ACHGNS_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFN6937717.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSEISTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5193..5549
                     /locus_tag="ACHGNS_001777"
     CDS             5193..5549
                     /locus_tag="ACHGNS_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937718.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5594..6709
                     /locus_tag="ACHGNS_001778"
     CDS             5594..6709
                     /locus_tag="ACHGNS_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937719.1"
                     /translation="MKNYHLSCSLKKGNPIEGEEYRIRYFFHLMYWQSFDENHSDILS
                     SIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSITYVPEEMNKFVHPL
                     ITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYLEEDLLDTPDKVQES
                     WGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGTKKKVDAFGKSAEDR
                     DFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCMLVRDIFIKHEQPVIF
                     YIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYPIDKKAYDQTNTQIF
                     YWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6735..7175)
                     /locus_tag="ACHGNS_001779"
     CDS             complement(6735..7175)
                     /locus_tag="ACHGNS_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFN6937720.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7335..7841
                     /locus_tag="ACHGNS_001780"
     CDS             7335..7841
                     /locus_tag="ACHGNS_001780"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFN6937721.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            8009..9922
                     /locus_tag="ACHGNS_001781"
     CDS             8009..9922
                     /locus_tag="ACHGNS_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFN6937722.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9927..10928
                     /locus_tag="ACHGNS_001782"
     CDS             9927..10928
                     /locus_tag="ACHGNS_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN6937723.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(11130..11336)
                     /locus_tag="ACHGNS_001783"
     CDS             complement(11130..11336)
                     /locus_tag="ACHGNS_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFN6937724.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11982..12830)
                     /locus_tag="ACHGNS_001784"
     CDS             complement(11982..12830)
                     /locus_tag="ACHGNS_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFN6937725.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12994..13896
                     /gene="gnd"
                     /locus_tag="ACHGNS_001785"
     CDS             12994..13896
                     /gene="gnd"
                     /locus_tag="ACHGNS_001785"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFN6937726.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            13898..14899
                     /locus_tag="ACHGNS_001786"
     CDS             13898..14899
                     /locus_tag="ACHGNS_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFN6937727.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14896..15180
                     /locus_tag="ACHGNS_001787"
     CDS             14896..15180
                     /locus_tag="ACHGNS_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFN6937728.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15213..16010
                     /locus_tag="ACHGNS_001788"
     CDS             15213..16010
                     /locus_tag="ACHGNS_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFN6937729.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            16024..16857
                     /locus_tag="ACHGNS_001789"
     CDS             16024..16857
                     /locus_tag="ACHGNS_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFN6937730.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16875..17732
                     /locus_tag="ACHGNS_001790"
     CDS             16875..17732
                     /locus_tag="ACHGNS_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN6937731.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17754..18263
                     /locus_tag="ACHGNS_001791"
     CDS             17754..18263
                     /locus_tag="ACHGNS_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFN6937732.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18300..18701
                     /locus_tag="ACHGNS_001792"
     CDS             18300..18701
                     /locus_tag="ACHGNS_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937733.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18813..19166
                     /locus_tag="ACHGNS_001793"
     CDS             18813..19166
                     /locus_tag="ACHGNS_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937734.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19200..19643
                     /locus_tag="ACHGNS_001794"
     CDS             19200..19643
                     /locus_tag="ACHGNS_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN6937735.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19660..20745
                     /gene="uxuA"
                     /locus_tag="ACHGNS_001795"
     CDS             19660..20745
                     /gene="uxuA"
                     /locus_tag="ACHGNS_001795"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFN6937736.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20738..21451
                     /locus_tag="ACHGNS_001796"
     CDS             20738..21451
                     /locus_tag="ACHGNS_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN6937737.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(21658..21807)
                     /locus_tag="ACHGNS_001797"
     CDS             complement(21658..21807)
                     /locus_tag="ACHGNS_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937738.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            22203..22403
                     /locus_tag="ACHGNS_001798"
     CDS             22203..22403
                     /locus_tag="ACHGNS_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN6937739.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         7746 a         3451 c         4641 g         6626 t
ORIGIN      
        1 tccatacatc cattgtactc tgagaaatca cacaatatcg aaacaaattc cgttaggaat
       61 gtgttattca ataaatttta taatctagaa acattttgaa taaaaactat tagacaaatg
      121 atattttacg aggaggaaca gagatgacga tctttgaaac ggttgtaaca gatgttggta
      181 tagaagcaga agtattcagg aaagaaaaaa taatgatttt atttggagat gaagcaccgg
      241 aaacattaac agattattgt tataagattg aattgaatca ggtaagaaaa gaaataactt
      301 gtgatatgaa gctctgtttt gataaacaaa tgtatgtaat cacagcagta ggagatgttg
      361 tccaaactaa tttgaatgaa ttaggacata tcacgataaa atttgatgga tctcatgagc
      421 cggaacttcc aggaacaatg tatgtagaag ataaggaaat gccgcattta agaaagggga
      481 caatcattaa aatcgatcaa gaggatcaga atgggggaaa gctatatgga aatcatgctt
      541 gatacagcaa atacaaaaag tatttattct tgtcaacagt ttttgaattt aacaggcgta
      601 acaacaaatc ctacaattct gaaaaaagaa ggagcgtcaa acttcttctc gcatatgaaa
      661 gaaatcaaaa aaataatcgg acagattcct atccatattc agacaatagg aagaacggaa
      721 gaagaaatga tagaggatgc tcgagttatc atagaagaac tgggaaaaga tacgtttatc
      781 aaggtgccgg tgaatcaagc aggccttcaa gcgatcaaaa agttgaaaaa agaaggttat
      841 ttgattaccg gtacagctat ttacacagaa ttacaagggt atttagctat taataatgga
      901 gcagattata ttgcacctta ttataacagg atgcaaaatc aaggcattga tgctatgaaa
      961 gtgattgatt cgttgcggac tgagatcgaa cgtacgcaaa gaccaacaaa aatattagca
     1021 gctagtttca aaaattgtga acaagtcact acagccattc gatctggagc tcagcgataa
     1081 cgttaggtaa agagctacta gatcaagtat tagagatgcc ctcgattgat aaagcagtga
     1141 ggggcttcca agaagattgg gtgcaaactt tccaagcaga aacgattggt gaactaaaaa
     1201 aggtcgctga ttacttctga atcaatttct ccataaaata aacgatagcc ttctcgaaaa
     1261 ctcgaggagg ctatctgacg attcaatatt cattaattta aagtttgggt gactattact
     1321 aactattact tatttctcat ttaaacgatg tattccctaa cctctaatac atccttgtaa
     1381 ctctgaagtt acgccagttt tgaataaatt tcataaaaac aaatgaaaag tgtcgcataa
     1441 aaatatcttt cgtgtttctc tagtataaca gaataagaag aacaagtcca gaagggatat
     1501 ttttagtctt ttttaaaata acttttcagt cgagtttgag tttatgatag ttgtttttga
     1561 agcgatacgg caagtgaaaa aatagggaag catgaaagaa tctacttaat ggcgcattga
     1621 ttcatgtgat agaaataaaa aactttttta aattatatcc tcatgattgt aacaaatcca
     1681 gtgatttgtt ttggtacaaa taaaaaaagg ccccgatgaa gttgtggcaa aagtggtttg
     1741 cctcaaaaat aagaaggaat ttccaaaaat cacttctcaa attttgccac atccccaatt
     1801 gggtcaaatt tgtttatttg aatatatttt ttacataagg atctttaata gttacattgt
     1861 cttttttcat cactttacgg attgttttat ccatgaattc cgtatcaaga gtttttgctt
     1921 cggtagcaat tttttctagc tcagatttgt atttgtcttt attagaacct ttatcctgtt
     1981 tttttaccat ttttactaga taatagcttt gctgataagt cgttggatct gttacaggaa
     2041 tgatatcaga aacttccccg ttttttagtt taaatgctgc tttttttact tcagtaggaa
     2101 tctctggatt ggttgaatca aaattcattt ttccatcagt ttcctttaat gttttatttt
     2161 ttccataagc ttgaacaagc ttggagaaat tttctccttc atttgcttct ttttttgctt
     2221 tatctgcttc ttctttagtc gaaaatagaa tgatctgtgt actaacttct ggatagaatt
     2281 ctttccaagc agtgtctaga tctttatctg ttaattttac attggctttc aaaccgtatt
     2341 ggaaagccag catctgttta aagagtttct tatatgtctc ttctgtttgg ccaactgctt
     2401 ttaattgttc cttgtagttt ggaccgagtt ttttgatttg ttcattatat tcttttgtta
     2461 cttcctttga agaaatctct tttccgtata catcatcaaa tgctttgaag acaatcattt
     2521 tttttaatag tgtttgatta tttgatttaa cttttgggtt atcataaaaa tcctgaactg
     2581 taagtgttcc acctttaaac gttgcaatcg tttcattgga cttgctacaa cctgagaata
     2641 gtaaaattgc acatgctgaa aggcacataa ttagtagttg ttttttcaaa aaatttccca
     2701 tcctttcctt attaaaaaca gtctaacaga agaattaggg agacagtctt tcattttcgt
     2761 aaggttgctg tagatattct tagttcgtaa aaatctattc tctctatgat ggagaacatt
     2821 tacatagttt tcacgagaca agaaagaaaa gggttgttaa acttaatata aaggagggat
     2881 gaaagatgat caggattatt cggttgattg gatctgtttt aatagtcttt gttggttttg
     2941 ttttaggaat tcttgtcaat aatgcacttg cggatatgtt tattggaaat agtgaatgga
     3001 ttggagcaat tgccggtacg gtcggacatt tagctgtttt tttactagct ttatttcttg
     3061 ctagtaaggt cgaaggaaaa agaatagaag attatggtgt ttctgttcga ggaaaagact
     3121 gggggtattt agcgggaggt ctcgttgttg gaattggagt gtttctgctt attactagtc
     3181 cattatattt gacgggggcg tatcggttag attcaggaaa cgcaaacatt gtgccgctga
     3241 taacgagttt tattttattt attgcagtgg gggcatcaga agagctttta ttccgtggct
     3301 tttttcagca tcagatgctt agatttggtc cgttaatagc aatgattgga tctgctgcat
     3361 tatttgctct tttgcacgga ttgaatccta acatgacatt tctggcagtt ttcaatatct
     3421 ttttagcagg ttgctttttc agcgcactta tctacagtac aggaagttta tttacggcta
     3481 ttggtgctca tattacttgg aattggacac aaggagctat tttgggtata ccagtcagcg
     3541 gaacgcaaga acaaggtttt ttctccactc tgatccaatc gcaaaataca ataattactg
     3601 ggggaaactt tggggctgaa gcatctatat caactacatt attcttgtta gtcttgattg
     3661 ttggtttgtt actctatgca tataaaacaa agcggatgaa gaaatacact gctgtttatc
     3721 aataaaagat gaagaaagca aggctgatat cggtcttgct tttttaagtc tgtcattgat
     3781 taaacgaaaa agtaaacaaa ttcaagcgaa tacacgacat cttttgttag attttcacaa
     3841 ctgactgtcg tgttctcatc attttcattt tatataattc ctttatcaaa tagaaggtaa
     3901 ttatatggag agtgatagaa atgaagaaaa cagcgaccgt attatttgtt attcttgggc
     3961 tgagtataat tgcagcaatg attggaatca gtacttctgc aaatgaacgg gcaaatgaga
     4021 atctttcatt tactgtgaaa actgatcgga ttgtttatga catgacacaa caagtcatta
     4081 ctattccagt caaaccaaac aaaagcgtga atgctagtga tgtacatgct gttctaacat
     4141 atggttggga tggtaatgga agttcagaaa aagttattgg ggaagtttat ttaaaagacg
     4201 tacaatggac cgctggtatt gagtatacaa taatgatttc tgcagaacta tcgattgatg
     4261 agatcaagag taaagataaa gttgatttga ttgtttttta tgatggacag atgacgatca
     4321 cggagaattt gaaaccaagc agttggacag ttgttggacc gccaagcagt acggatagca
     4381 caagtagtga aagcagtaca gaaaacacga gcggtgaaag cagtacagag agtacgagca
     4441 gtgaaattag cacagaaaac acgagcagcg aaagcagtac ggagagtacg agtagtgaaa
     4501 gtagcacagg aagcacgagc agcgaaagca gtacggagag tacgagtagt gaaagtagca
     4561 caggaagcat gagtagtgag agtagcacag gaagcacgag cagcgaaagc agcacaggaa
     4621 aagtaaataa tgaacttgat ttggtaccta tcataccaac taaggaacaa aagagaaatg
     4681 cagggagcaa cctaaaaagt gtgctaaaac agcctgattt tgtttctcgt ggaattgatc
     4741 atcaagaaaa agcgaaagaa acagataata gaaaaagcga tttaccagaa actggtagta
     4801 gaaccttgaa caagctaatt acatggatgg gtgtgttact cattttgatt gtaggagcaa
     4861 gctattttcg ttctctcttc catcgagtga aataaagaat aaaagcaaca gatctaaaat
     4921 tgaataaaac gatagatttt tgagtgaata tttcattcga gagtgctgta ttgcctggcg
     4981 aagttgtagc tttgccagat ctatttttgt ttactaacat atttgagaaa caggaaataa
     5041 caggaacaca aatggaaaat aagagcaaga ataattgaca atgcacactg cgcatggtag
     5101 tatctctgaa aaatcagtaa cgaatctatt tgtacgttat tcatgtagct gtcggaataa
     5161 gtctaaataa tagataaaaa ggagtatgta ctttgtttga agaatttatt gatatcaatg
     5221 aaaggcaagt atatcaattt ttgaactatt gttatgaaag agacgagaaa ttatatgtgg
     5281 tgaaggatat cgctttagat ctaaactaca ccctggctaa gatgaattcg gtgattcaac
     5341 aagcagaatc gttttgcgaa aggtatccag aatataaatt atcttttctt tcagaaaaca
     5401 agatgataaa agtcgagttt tcttctcagt ttttactgag taaagtttat tctatacttt
     5461 tagagggaac gattggttat attttactgg attcacttta caaaggtact tatcaatcct
     5521 tagaaaatct tagtcaaaaa attatataag tttgcgtacg attcaaagaa aattaagaga
     5581 acttaatact attttgaaaa actatcattt atcttgcagc ttgaaaaaag gtaatccgat
     5641 agaaggagaa gaataccgta tccgatactt ttttcacttg atgtattggc agtcattcga
     5701 cgaaaatcat tctgatatat tatcaagtat aaaaaataaa cgtcaactat tagaaaaatt
     5761 tgcgaaacat gcgccatata cgcgtagtat agatagaaag aaatggatca gcctactaag
     5821 aatttcgttg caacgaatga aacaaggcca ctcgattacg tatgtcccag aagagatgaa
     5881 caaatttgtg catccattga taacaaaaga agagtttacg cattacatac tagagccttt
     5941 ttttgaagca aacttctggt cgatgaatca catcactcca acagagcaag cttaccttta
     6001 ttttatgttt ggtgtaatga acacctattt ggaagaagat ttgttggata ctccagataa
     6061 agtacaagaa agttggggct ggattgaatc gtctattgta gaaaattttg aaaaaaactt
     6121 caagatttgt tttacaactt cagaaaaaaa ttatctatat gtcaatttgg caatgatcca
     6181 tttgtattca agggtttttg ggacaaaaaa gaaggtggat gcttttggaa aatcagcaga
     6241 agacagagat tttcagcata tatttccagt gatgtatcca atcatgcaac agttttttag
     6301 tggggtagct cgcagaattc cagagcttaa tacttattat gaaaagaaca gacggttgat
     6361 ttttcaatac tgtatgttag tcagggatat ttttataaaa cacgaacaac ctgtcatatt
     6421 ctatattcaa tcaaagtatg gtaaaacaca ggaattatgg gcaaagaaaa ggatattatc
     6481 tatgactgaa cgcccaataa aatatagtgc atccgctgat cagttacctg atattgtcat
     6541 tagcgattat cctatcgaca aaaaggcata tgatcaaacg aatacacaga tattttactg
     6601 gaatggacaa cctacaaaaa atgattgggc gcgtctgaaa gctgaaatca taaaaatcag
     6661 gaatgagaaa aatagatcca tattggaaaa attggatttc gttaattaag aaaagcagat
     6721 tgtttatgcc aaaattaaga cattgcaaaa agattatttt ctttaaaatc gacaagatga
     6781 ccagaaagca agagttcgtt gagctcttgc tttcttttag ttttagaaag gaacatcctg
     6841 attttatctt cgctaaatcc agtcaaaagt tttgcagatt ggtcgtagat aatcggtttt
     6901 ttcaataaag aaggggatgt acgaatctca atatatagtt cactcaaact gagttcatct
     6961 attctgtgaa caagattttt ataactaata gaatgagtag agagcatttc ggtcacgccg
     7021 ttgtttgtca attttagtaa gtggataaat tcatttttgg ttagtggttg acgaattaag
     7081 ttctgttcga taaacggtat atcatggacg ataagccaac gttttgcgcg tcttgaagac
     7141 gagcagccag aacctgtata caatttaatc atcatacatt cctccaatag ctaagtattt
     7201 atgaaataat aacatgaaga aatagtcgaa aagtgccatt tagaggtgta aaataagaaa
     7261 atgttgtcac gtataatgga actagctaga taagaaatac tattaaatca agaaaaaaga
     7321 aagaaggcac aagtatgtca aacacagccg acgcaatagt agtgatcgat ttacaaaaag
     7381 gggtatgttt tgattcttcg ccgatacacg aattagaaac agtgacagca ttagtgaata
     7441 aaagaatcaa aacatacgaa acgattggga aaccagttat tttcgttcaa cacgaagatg
     7501 ctcaattagt gccacaaaca aaaccatggg agctattgtc ggaattatct attcctgagc
     7561 acagttattt tgtgagaaaa acccatgcga attcatttta taagactaat ttgagtgttt
     7621 tattaactga attggactgt cattcgatcg aattatgtgg ggcacaagca gagtattgta
     7681 tagatacgac tgtgaagttt gctcatggat taggatacca gcttatgatg tgtagaggag
     7741 caacgacaac ttatgataat ccttttatga aagcaaagga aacaatcgct ttttacgaga
     7801 aaatttggga taaacgattt ctgacatttt tggagcaata agttgtttct tcgtttaaaa
     7861 aaagcagaat ggatactggt ttatccattc tgctttatta cgtaaaatga gaaaataatc
     7921 ctgttctatt tttatgggat aaaccattta ttttttgtat aaaaaatgtt tgcgtttaca
     7981 tataattctc ctagaaacat aaaaaggagt gaatagcttg agaacaaaac aagtattaaa
     8041 gggagcgttg gtcatcttat ttgctgtaag tacaagcgtc ccgcaatgga cagtatatgg
     8101 agagagcatt tcttccgaat cggaatctcg ggaggtagaa accacagaaa ctactgatgc
     8161 tggaaatgaa acgaaatcga gtacttcaca aacagaaaca aatactgaaa caacggttga
     8221 gacatctgtt actagcacag atcaagaaac gaccgctacc acagataacc aacaaacaga
     8281 agaagcaaca gctacaaaaa tgtcaaacga cgaatcctct tctgctaaag taagtgacaa
     8341 agagaatggg acagaaaatg atgatatcgg taaaggtgaa aatttcaatg gtaaagaata
     8401 tttgaattac ttcggtaaac agatgacaga aatacttaat ggaccaacag aagacatcaa
     8461 gtcaaaatta atagagttat tagtcactga gcttgctgtt aatgattatg gtgtagataa
     8521 agagattatc aaacatctga ttgctactta tctattgtac gatatcgctt ttgcaaaaaa
     8581 cagtgtgttg atgtcgatgc ttggaggtac agatgatggg acaacgccta cagaaaacac
     8641 aggcttctat ccaaatacaa aacaacttca tcaaactgta ctaaacaaac aagatggcaa
     8701 aatgatggat gtatatgcat attatgtcga tcaaggttca gataaaacag tcatgatcca
     8761 tggcggattt cgagggaatt ggaataacgg gatcgttaca gaagaataca atgattttta
     8821 taaggccggg tataatttat tatttgttga ttccagagcg actggtaata gcggaggcga
     8881 ttatgtgacc tatggacaat atgagtcaga tgacgtcctt tactggataa atcaagaagt
     8941 tcgagagcgg ccatctcaaa agattttact ttatggtggt tcgatgggag cagctacgat
     9001 gatgtctgta ttagcgaaag atattcctgt caatgtcaaa ggaattattg aaaattgcgg
     9061 atttgcatca attgatgaac aactacgttt tacgtacagc cagacagtag cccccgcact
     9121 accggatgcc ataaaaaatc agttagacat cattggggat caagagcatg aagatttatt
     9181 tatgggcctt ttgaaacaat actattttga tcaggagatg catttggata caaaagctgc
     9241 tctacctacg attggaatgt cagattcatt gcctaaattg attatccatg gaacagcaga
     9301 tgatgtagta cctgtgtcta acgcgcaaaa attatatgag ttatcaggag ggtataaaga
     9361 tcttttatta gtagaaggag cagggcatgg aaaggcacag gaagttgacc atgctgccta
     9421 cacgaaacat gtaacggact ttttgaaagt agtgttccat gatcagataa acgtaaagta
     9481 tgtggatgaa aacaataaaa gtctactcaa agaccaggac gaaatccagc tttacggagc
     9541 ttatggagaa aattatatga cagagcaaaa gacattcgaa ggatacgaat tagcaaatgt
     9601 tgaaggaccg actgaaggga tttttaatga gacgatacca actattattt tctattacaa
     9661 aaaaatccca gtagctccac caaaaaagca agatccgaca gagaatgcag acaataaggg
     9721 aaagaatgaa cctcgagagg atcaatcaag tgatgatacg caaccgattc agatgacagt
     9781 gaaagtacaa aaaaagacag atgcaaaaaa aagaatgttg ccgaaaactg gagaaaagaa
     9841 acattcgctt ctcatttatt caggtggatt tttatctgtt agcacgatta tcacatggat
     9901 ttggcgaaga aagagagggt aaatgaatga aatggttcaa gcgaatagtg attgcagtac
     9961 cagttatcat cgtagttatt ttagtcactg tatttttgat taatcaaatc acaccaaagc
    10021 cgattagctt gcttgttaga aaacaatttg acacaagtga aaaagaacaa gtatatgccc
    10081 gaccagatga tttccagcaa aaaatcgaac aaatcgatat aaaaaaagaa atatcctatc
    10141 cttcaaagta caaaaataat aatatggata tctacacacc gaaagtggaa aagaaaaaat
    10201 taccgattct tttctggatg catggaggcg catatgtagg aggggataaa aatgattgtc
    10261 gtgattattt ggaatatcta tgttcagaca cgcagcaaat catcgtaaat atagattatg
    10321 aacgatcacc ggaagcaaaa catcctacac cagttatcca attgaacgaa gccattcaag
    10381 cagctaaaaa agagataaaa gatcaagcag attggtcgaa tatttcgatt ggtggaaatt
    10441 cggcaggtgc acaaattagt ggagaatatt tgttagcact tcaaaatgaa gaaattaaaa
    10501 aagctgatca tcttgatccg actttggaga acaagcagat tacgaagttt atttctttgt
    10561 ctggtctgtt ggatccttct gcacttactc aagtaagtga taaaatctct agctttcttt
    10621 atgaaaaatg tggatgggct tactttgata ataaggactt tgaacaatca gagaaggtca
    10681 aaagtttagc cttggtcaga catgcggatc gttggacgca aaacacattc ttaacggatg
    10741 gaaatacaaa tacgtttacg aaacaaatgg aagaagtaac ttctgtattt gagaaagcgg
    10801 gggtgaaagt cacaaaagta gattatgcgg agaaaaatgc tgttttgaac catgaatatc
    10861 agtttgattt cagcaataaa caagcgcaag aaacatatca aaagttagta atgtttttca
    10921 aggagtaaag tcttatgatg caaaagaggt tgtaatctgt cacttagctc caagtcacaa
    10981 ggagcaattt caataaacaa gcttttcatg gtttatgaat attgtaactt gtgactgagg
    11041 agttagagat ggcaaggggc accttttatt cgaaatttgg gaatatcaca cgattattgt
    11101 cacgatctct tttacgtaaa ttttataagt cagatttgtt gttttgatcg tttaaaaaaa
    11161 gcaacaaatc cgaagcctgc ggaggcaaga agggacaaaa agccgagtgg taagcaaaag
    11221 aatgctggtt ccactaatat tccgttgtta ttgatataag gctgggaaca aagatgaata
    11281 gcaaataaaa tcaatgcact agcgaaaaaa agcaaagaaa tatggatagc tttcataagc
    11341 agcacctcct tttttataat tgtatgataa atagattaag aatctataaa tgtgacaagt
    11401 ttgttaggat gtgaataaaa tgatcatcta gcgaacttga aacaaagatg gctaatgata
    11461 agcaattaat gattcagcaa tcagataggt acaaagttta ccatcatacg tctagaccaa
    11521 taatagaaaa tatgggaata taatagaaaa gatttaggat acccccaaaa gaatttcccc
    11581 aaaaattcga tgtatcctaa acttttttat tttacataac aatcatatca actgtgaaat
    11641 aaaattacaa atgataacct aaaatgaggc caaaagccag tgttatcaag gaaatactta
    11701 attcaccaaa atttttttta attttttaga aaataagaaa caaaaataat aaaaaaaaga
    11761 aaagataaat tttttactac tgtatactaa ctttgttatc gcatctaaca agtaactcga
    11821 ataataccaa tacaaccttc tccaaagatg cgatgacata cgttctaacc caccgactat
    11881 tgatgttctg taccaccctt gcagaaccaa aaaagccact accaaatatt tggtagtggc
    11941 tttttttgtt ttatggattt ttcgcttttt ctgttatggt gctattcttt gatttcagaa
    12001 atagtatgtc gaactttagc gatagaacga cgtgattctt tttcgttgta aaacatcaaa
    12061 atcacatata gagagtccaa aatgcttagt tgagaaatac gggaagctaa agcttcagat
    12121 cggaattctg tttcttcaga aatagaaata aaaacgacat cgccaagttt agcaagggaa
    12181 gaattagctt ggctagtaat aacgattgtt tttgctccag tttttcttac ggtttcagca
    12241 atatggattg tttcttttga ttgccctgtg tgagaaatca aaacagcaca gtctttttcc
    12301 gtcaataaag aagcttccat caattgaata tggtaatccg tgctgtgaga agttgcgata
    12361 ggggaacgca aaaatttatg ataagcgtca gttgctacaa tctctgaacc gccaacaccg
    12421 aaaaaataga cacgtttaga ttcattgata atagtagccg ctttttttag atccttttgt
    12481 tgtaacaact tttttgtgtc atttaaagtt gaaatactag actcgaacac cttttgcgcc
    12541 atagtgagct cagtatctgt tttctgaata ttttcgtgga tagaaatagc agatagatca
    12601 ttttcttgga tcaacatggc cattttaaaa tctttaaatc cattataacc aagttttttt
    12661 gtaaattgaa aaaaagtaga gtcagcaatg cccagttcac ttgctaaatt gctaatagga
    12721 ctatgactta ctttgcttgg attttctaaa atataatcgg cgatagcctg ttcttttggg
    12781 ctgaagtcgt tgtacattgt tttgatttgt agtcttatgg agacgctcat gagaattcct
    12841 cattttcata ataagattat cattttttcc gttctcatta atgttaaaga tacaaggaga
    12901 gtaggaaaaa atcaaacaaa aaatattttt gtaaaatcta tttacaaaaa aatttttttg
    12961 tataatgagg gtataagaaa tggaggcgct atcatggaaa ttggtgtaat tggtttggga
    13021 aaaatgggat tgaatttagc tttaaactta aaagagcacc atcacaaagt acaaggactc
    13081 gacatttcaa gtacagctgt tagtcaggca agagaaaatg acattcctgc ttatcaagag
    13141 atctctgaat ttctagctac ttttgagcag aaaaaaatca tctggttaat gcttcctgct
    13201 ggagagataa ccgaaaacat gttaagaaaa ctttttccaa aactcggacc aggagacatt
    13261 attattgaag gcggaaactc ttattataaa gattcaatcc gtcgagcaga agagtttcag
    13321 aaaaatgaaa tcggttactt ggattgtggg acttctggag gaatcgaagg agcacgacat
    13381 caagcctgct taatgattgg aggagataaa gaaacttttc tatccgtgga acaattattc
    13441 aaagatgtag caattgaaag aggatatctg tatgctggtg catccggaag cggacacttc
    13501 ttgaaaatga tccataatgg aatcgaatat ggaatgatgc aagcaatcgg tgaaggattc
    13561 caacttgtac aaaaaagtga gtatgctttt gacttggaac aagtcgcacg tgtttggaat
    13621 catggttcgg tgattcgcgg ctggctgatg gaaatcatgg aagatcagtt aaacgaacat
    13681 cccaatttaa gtaattatcg gggaatcgtt tctgcatcag gtgaagcaaa atggacggtt
    13741 gaaacagcgc tagaaatggc tgttccagtt ccagtaattg caactagttt gttcatgagg
    13801 aatctttcac aggaagaaga tagtttttca gcaaaagtag tttcagcact aagaaatggg
    13861 tttggcggac atgaaatagt tgaaaagggt gagtaaaatg ccagattatc atatagcaat
    13921 cgttaattcc agcagtttcg gaagagtctt tccacaccac ttggaaagat taaagtcagt
    13981 tggagacgtt catttcttta ctgttgatca agagattcca ggaaaagaac tagcggagtt
    14041 acttaaagga ttcaatatca ttattgcaag tgttacgcca ttttttcgac gagatttttt
    14101 tgaaaacaaa gatgaactgc tattgatttc ccgacatggt attggttaca acaatattga
    14161 tttaaaagca gcaaaagaac accagacaat cgtatctatc atacctgctt taattgaacg
    14221 aaatgctgta gcagaaaata atataactaa tttgttgtcc atgcttcgac gcacgacaca
    14281 agcacaagca agagtcaagg acgatcgatg ggaagatcgg gcggagttcg tcggtaggac
    14341 gctgtttaac aaaacagttg gtgtgattgg tgcaggaaat accggaagcg gagtaatcga
    14401 aattttgcgt aacggttttc gctgtgatgt tctagcttat gatccttaca aatcagattt
    14461 atacatcgaa agctttggtg caaaaaaagt agaacttcaa gaattactgg aacaatcaga
    14521 tatcatttgt ttatgtgcga atttgacaga agagaactat cacatgattt ctgataaaga
    14581 agtcgatcag atgaaagaaa atgtttatct atctaatagt gcaagaggcg ctctagttga
    14641 tgaagaagcg atgattaatg gtctgaaaag cggaaagatc gcaggatatg ccacagatgt
    14701 tttagaagaa gaaccgggac gtcaagatca tccgtattta gcgtttgaac aagtagtgat
    14761 tacgccacat atatcggcct acacaaggga atgtttagaa gcaatgggtg aaaaatgtgt
    14821 cagagatgtc gaacagatca gtcagcacct tcttcctgaa cgttctgtcc agacaaagag
    14881 caactatata aaaggatgat gaagaaaatg gtgaacggtt taaaagtgaa aacaggccct
    14941 caattttatt tatatgaaga aggtggtatt tcaaaagttt ctgatctctt gaaatcatac
    15001 ggcgcgaaaa gagtactggt cacccacgga acggtatcat gggaaaaggc gctacctaaa
    15061 ttagtatttt tgaatgacga aacgattcaa tttttttatc atcgttattc tggagaatgt
    15121 agttatgctg aagccagacg aatagctaca atcatcaaaa aaatgaaatc gatttcgtag
    15181 ttggcgttgg cggaggaaaa ctgaccgatc tagtgggtta tagtgccaat cttgcaagaa
    15241 tcgatttcgg agtcatccct actttagcta gtaattgtgc tccatggaca cctcttgctg
    15301 tcatgtataa ggagaatgga caagcagaag ggaaaacaga acacttttac agacaagctg
    15361 ctttttttat aacagatccc caattagtga ttgattcccc tgttccatat ttcattgccg
    15421 ggttagcaga tacactagca aaatggtatg aatctgagct catcttagaa caagattttt
    15481 tgcaagatga atcatttttg aagctagcac aagatactgc gaagatctgc aaagaagaga
    15541 ttttgaatca ttctgctaaa gcaatccaag atatggcaca aagaaaactg acatcggagt
    15601 tcaaacagct ttcagagatt atctttgctg ttgctggttt agttggagga ttaggagata
    15661 aatacgcaag aaatgcggca gcacatgcta tgcatgacgc tatcagtaaa tatattccgg
    15721 aaagccatcg ttttctacat ggagaaaaag tagcgtatgg gattttctat caactggcac
    15781 tagaaggtcg ctgggaaaaa atagacgagt tatatccttt gtatcgggaa ttagaactgc
    15841 cggtttctct agaacaaatg ggattatttc cgaaagataa acagataata gataaaatgg
    15901 ctgaatttat tgattcgaaa gagaaagtgc atttgattcc tatagaggta acacagaaat
    15961 cactgataca gtcaatgatg gaactggaag gttatatcag aaagatctga aggaggagga
    16021 cacatggaaa cattaagtgt agtacaaagt ttgttgattg ctttatgggt agccgcggtt
    16081 atgtcccgct ggttaggtgg aggagccacg ttgaccttac gtttttctcc tttgatgact
    16141 ggattagtcg tcgggttgat catgggggaa gttgcacaag cgatgattgt tacagccgct
    16201 ttgcaaatga tttatatggg ggttttttct ccaggaggat caatgccagc tgaaccatct
    16261 attgcagcag ctatcgcagt accagtcgca ttattagggg atctgtcgcc agaagcagcg
    16321 attgccgttg cggtgccagt cggattgctc ggaagttact tgtatcagtt tcgttttttc
    16381 atcaatactt ttttaggtaa atatacagac aaagctgtag aagatttgaa tacaaaagct
    16441 atggcccgat caattattct ttatccaacg gttgcctcat ttttattatt cgttccgctt
    16501 gttttcattg ctttatattt aggggcgcca ttgatcgctg atgtaatcaa agctttagag
    16561 ggaacagtcg ttatccacat tcttgaagta gtaggtggag ggttagcggc aatcggtatc
    16621 gcgacaactg tatacgttat cggtcgaaaa gattatctgg tctttttctt ccttgcttat
    16681 tttatgagtg ttgttttgaa atctttagat attacgatgg ttacttatgc catttttggg
    16741 attattatcg ctttgatctt tgtccagtcg caaaaaggga aacaagtaca gcctgccaca
    16801 agcaataaca attcagcatc ttttgatgac gaagatgact atgatgacgg attttaattt
    16861 aggaggaatg ggaaatggct gaaataatgg ataatcaaac aggtgctcct gaagaaatca
    16921 cgaaaaaaga tgtaacaaaa gcctatctga gatggcattt tgcaaacgaa attcctcact
    16981 cttttgaacg ttacttggcg ccatcactac tatacggaat gatgccaatc ttgaaaaaac
    17041 tttataaaga tgaagactct ttaaaagcag catataaacg gcagttactg ttcttcaaca
    17101 ctcaattaag ctggggtggc ggtgtgatta ctggcctaat gtcttctatg gaacagcaaa
    17161 gagcagaaga agagtatgaa gaaaaagaga tcttgatgca ggatgacttg atgtataaca
    17221 cgaaagcggg attgatgggg gcactagcag gaatcggaga tgcaatcgat tcaggaaccg
    17281 tccaatatat ttttatcgca atcgcggtac cttgggcaca acagggaagt cctttaggcg
    17341 cactttttcc ttttatctgc tttgggttgt atcaggtttt actaggggta tttttcgcaa
    17401 gacaagcttt cactatgggc cgtaatgcta caggcctaat gcaaaacact ggtgttcaga
    17461 cagcgattga aatgctatca gttttagggt tgtttatgat gggggtgctt gcgggaaact
    17521 atgtaaaagt agaatcttca ttgaaatttg cgatatccgg acgtgagttt gttatccagg
    17581 aaattcttga tcaaatcgtg ccagggatat tacctttagc tgtcgtaatg ggtgtgtact
    17641 tgttctatac gaaaaaagga ttgaaagtca cacaagcatt attatggctg actggaatat
    17701 taattgtgct agcaggaatc ggtattttat aaaaagaagg gaacgtgaat aagatgggtg
    17761 tagtcaattt agcgcgtgtg gatgagcgtt tgatccatgg gcaagtaatg gtaacgttat
    17821 ctcaaaaaag tggagtgaat tccatttttg ttgtagacga agttgtagca aaagataaat
    17881 ttatgagaga tctttacaaa aatgcaggaa gccgtacagg acaaaaaaca attgtcatta
    17941 caccagagaa agctaaattc tattgggatg aatatcaatt taaagaatac aattgtattt
    18001 tgatcgcaaa aacagtccaa acgatttacg acttagtaaa acacggaatc cccatggaag
    18061 aattgaatat tggagggatt gctcaaaaaa atcctgatac agatctttta gtgacgaaat
    18121 ctgtttattt aaataaatca gatgctgaaa aattgaaaga aatgaatgaa caatatggcg
    18181 taaagaatat ttattttcaa gccactccat ctgcacctaa aacaggatta aaagatgtat
    18241 tgaacaagtt tgatttgtca taaacaaatg cttagaaaaa agataaagag ggcgaaaaaa
    18301 tgaagaagaa cgagctgttt cgtgattggg aatttcgtta tcgctatatt taccgtaagc
    18361 gccgtacaaa aaagagtaaa cagcgttttc tttcagcatt agtatcagat atttacagta
    18421 tgagaacaga tgtgacagtc attgcttacg atacgctagc ctaccgatca aaaaatattt
    18481 atgttggaga tatcgaaaaa gcagagaaag ttatctgtac ttattacgat acacccgttc
    18541 atgcattagg aagttatttc atgtttgatt ggaaagacca aaggaaaaaa acaatctact
    18601 ctatattatt gtccttcatt ttgttgttca gtttaggttg gtggggaatg atgatatata
    18661 acaaaaatcc tcatcacgta ttcgatctgc tatccgtata aacaggtatc actgtactag
    18721 catttgggag ctactttttc ttattgggaa aagcagctcg cggatggcct agcagacaaa
    18781 cttttattcg aaatacttca tccatattaa cgatgctaga aatgatccgt acgattgatg
    18841 atccgagtgt agcgtatgcg tttgttgatg aaggctgtta tggagaaaag ggcttagaca
    18901 gcgtgagatc gggtatgaaa aaagaggcta ttctttttta tctagactct gttggggcag
    18961 atacaccgct acagttttcg ggaaattatt tttcaaataa agaacagtgg ctgaaacaag
    19021 ttgataagtt gaaagaaaaa aacgtcaatt atatttttag tgcaagaaaa aagcaagcgc
    19081 agttcttcta tttgaccaaa acagatttac gagggaaaac atttaattgg caaaatgcca
    19141 atcagattat tgctcttttt cgataagtaa aaagtgcaaa acaaggagtg agaagactaa
    19201 tgttggaaat catgatcgtt acacatggta agttgagcga aggactgaaa gatgcggcgg
    19261 aagtaattgt cgggacatcg agccatattg aaacaatgtg cttagagtct ggggaggaca
    19321 tccaaacatt cggagagaag gtcaaagaaa aattaactat gatcaacaca gcagaaccca
    19381 tgctagtatt tgttgatcta attagcgcaa gtccgtacaa tcaagcgctc ttggcagtca
    19441 atgaactgac tgaagaaaag aaagcagcca cttatgtcat agggggcgtg aatctgccaa
    19501 tgttgttaga agcaatcaat catcagatgc ttcacacacc gatagaagaa gcggtggaac
    19561 aaattgtcaa acaaggaaaa gataatttaa ctgtttggca tgcttcagcg gaagaaaaaa
    19621 aattagatga ggacgatttt tagaaggaag gctgaaaaga tgaagtgggg atttagatgg
    19681 tatggtgaaa aaggggattc gatcccatta aaatatgttc gccaaatacc gggaataaat
    19741 gggataattg gaactttgct gactaaaatg cctggagatg tttgggagat ttcagaaatt
    19801 gaggagttga aagcatctgt tgagagccaa ggactggaac tgcttggaat tgaaagtgta
    19861 gcgatccacg atgcaatcaa ggcagggaca gatgaacgag attactatat tgataactat
    19921 atacgaacaa tcaaaaactt gtctcgatgt ggggttcgat tgatttgtta tagcttcaaa
    19981 ccaatttttg gatgggcgaa aactgatttg tttaatgaaa acaacgatgg aagctattct
    20041 cttttatacg atcagaaaat cgtcgatcag atggagccag gagaaatgta tcaattgata
    20101 cacagccagt caaagggatt caaattgccg ggttgggaag aagaacggtt gaaaaaattc
    20161 gctcagctgg aaaaactgta tgaaggaatg accgaagaag aattatttga aaatctgact
    20221 tattttttag aaaaagtgat tccagtgtgc gaaaaagcag atgtaaaaat ggctatccat
    20281 ccagatgatc cgccatggga actttttggt tttcctagag tgacgaaaaa tcttggcgac
    20341 ttgaagaaaa ttctaaatgc agtagattct ccatataatg gactaacgct gtgcacagga
    20401 tctttagggg caaatccgga aaacgattta gttgaaatca tccatgaggt aggttcgcgc
    20461 atccattttg tccacttcag aaacgtggag tttttaggag aaagaaagtt caaagaaacg
    20521 gcacatccct ctacagaagg ctcccttgat atgtatcata ttatgaaagc gttggtgcaa
    20581 gaagggttcg acggtcctat acgccctgat catggtagaa ccgtgtgggg tgaagtggct
    20641 atgcctggat atggcctgta tgatcgtgca atgggactca cttacatgca aggtctatat
    20701 gaggcgataa gtaaggaaaa accactggga gagcattatg aataaaatca agctcttcat
    20761 ctttgatatg gatggccttt tattagaaac tggacgttta gcttatcgtg cttatgtaaa
    20821 atcagcacaa aagtatgatt atgaaatgcg taaggaagtc tattatttac taactggcca
    20881 aacagaaatg gcgataagag aacaaatggg cattttgtac ggagaagatg ttccatatat
    20941 caaatggcga gaagcaatca atcaatataa agaaaaaatc gtgaaagaag ataagagagt
    21001 atatacgaaa aaaggagcag aagaaatttt atcttttgca aaagaaagag gaatccatac
    21061 aatcgtggct tcgtctaata cacgggtaaa agtagaaatg tatttaaaaa tggaaaatct
    21121 atatgccttg tttgatcaaa tcatttcagg ggatgatgta aaaaaaggaa agccggaacc
    21181 agagatcttt ttgaaagctt gttcaaaaat gaatattcct ccaagtgaag cacttgtatt
    21241 tgaagactcg attgcaggga tcgaagcagc aaggcgagcg ggcattttgt cttttctcat
    21301 caaagatcat ttgatagggt tgcccgatca taatgggaag cataagttga aagtagatgt
    21361 aagtttatat gaatccaata aagcagacta tgaattttat gacctgcatc aagcaaaacg
    21421 atttttagaa agtaaagggt tgtatctcta ataaagagga ctattttcta gtcctcttta
    21481 tttattgtcc tgcacgactg agggaacaga tgaaaaagaa gtaatttttt attttgaagt
    21541 atctacctta tttgtagtga acaatttaga acaaacacaa aacgcatcta ctgttatcta
    21601 ccagtagatg cgtttaaaga gaagaacgag aatcgtccat atgagggttt ccactcctta
    21661 tgcagatttt tttaattctt gcactgcttg ttcgatcgta acacctgtcg ctacatgatt
    21721 ggtattttct ttgtcaaaaa cgacaaatag attcaaatct gtatccaagg ttactcgata
    21781 ttgtatatct ttgttataga atgtcatagc cattctcctt tctttaaata cttgctgaaa
    21841 ggcaacgaac gcgttgataa cgaaattcca tttataagta taccaccaat tgaaaggttt
    21901 tcatagttaa gaaattttaa agtcaaaagt tgcagataaa agcaaataaa caagaaaaca
    21961 aaaagtgaat caatcgcggt tattcaactt tttcttcgat tcgaagacaa tagaagacaa
    22021 tgaattttat atttcattgc attatccttg tgttattaga aaaagcatga tatattaaat
    22081 gagtaatcta ttttgaaacg taatttgagc gatatatcta catagataaa actccttttg
    22141 cctatcaaat atttattgca acgaaaaaac gtacattgat acgtatcagg aggaatttat
    22201 ttatgaataa cggtacagta aaatggttca acgcagacaa aggttttggc tttatcactg
    22261 gtgaagatgg aaatgatgta tttgcacatt tttcagctat ccaaggtgac ggcttcaaaa
    22321 ctttagaaga aggccaagca gtgtcttacg atgttgaaga tggtcaacgt ggccctcaag
    22381 caacaaacat tgttaaagct taataaaaat tgacttttaa ccgtcttttc gaaagaaaag
    22441 acggtttttt tgtacgaaaa aatt
//
LOCUS       DAZBTF010000046        22175 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000046 DAZBTF010000000
VERSION     DAZBTF010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.046"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            240..2075
                     /gene="tdc"
                     /locus_tag="ACHGNS_001799"
     CDS             240..2075
                     /gene="tdc"
                     /locus_tag="ACHGNS_001799"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFN6937740.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2380..3858
                     /locus_tag="ACHGNS_001800"
     CDS             2380..3858
                     /locus_tag="ACHGNS_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN6937741.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3824..5155
                     /locus_tag="ACHGNS_001801"
     CDS             3824..5155
                     /locus_tag="ACHGNS_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN6937742.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5269..7821
                     /locus_tag="ACHGNS_001802"
     CDS             5269..7821
                     /locus_tag="ACHGNS_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFN6937743.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7828..9267)
                     /locus_tag="ACHGNS_001803"
     CDS             complement(7828..9267)
                     /locus_tag="ACHGNS_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6937744.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9242..9424
                     /locus_tag="ACHGNS_001804"
     CDS             9242..9424
                     /locus_tag="ACHGNS_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937745.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            9613..9975
                     /locus_tag="ACHGNS_001805"
     CDS             9613..9975
                     /locus_tag="ACHGNS_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFN6937746.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10162..11019)
                     /locus_tag="ACHGNS_001806"
     CDS             complement(10162..11019)
                     /locus_tag="ACHGNS_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN6937747.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11139..12050
                     /locus_tag="ACHGNS_001807"
     CDS             11139..12050
                     /locus_tag="ACHGNS_001807"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFN6937748.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12057..13708
                     /gene="mdcA"
                     /locus_tag="ACHGNS_001808"
                     /pseudo
     CDS             12057..13708
                     /gene="mdcA"
                     /locus_tag="ACHGNS_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
     gene            13810..14109
                     /locus_tag="ACHGNS_001809"
     CDS             13810..14109
                     /locus_tag="ACHGNS_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFN6937749.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14099..15775
                     /gene="mdcD"
                     /locus_tag="ACHGNS_001810"
     CDS             14099..15775
                     /gene="mdcD"
                     /locus_tag="ACHGNS_001810"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFN6937750.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15779..16372
                     /locus_tag="ACHGNS_001811"
     CDS             15779..16372
                     /locus_tag="ACHGNS_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFN6937751.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16396..17472
                     /locus_tag="ACHGNS_001812"
     CDS             16396..17472
                     /locus_tag="ACHGNS_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFN6937752.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17497..18366
                     /gene="mdcB"
                     /locus_tag="ACHGNS_001813"
     CDS             17497..18366
                     /gene="mdcB"
                     /locus_tag="ACHGNS_001813"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFN6937753.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18477..19241
                     /locus_tag="ACHGNS_001814"
     CDS             18477..19241
                     /locus_tag="ACHGNS_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFN6937754.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19730..21823
                     /locus_tag="ACHGNS_001815"
     CDS             19730..21823
                     /locus_tag="ACHGNS_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFN6937755.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21848..22150
                     /locus_tag="ACHGNS_001816"
     CDS             21848..22150
                     /locus_tag="ACHGNS_001816"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN6937756.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         7179 a         3788 c         4725 g         6483 t
ORIGIN      
        1 tgtttgttcg ctttttttag gttatctatt ttagaagact gatttattga aaaattgttc
       61 gcttttttgc gttgaattat cagaaaaatc cgactttatt tatttttgtt tgtaaaaatc
      121 tacgtggatt tgatgttata aaaaaaaaat tctcattttt ggcgctatag tgaagaggtg
      181 ttacagataa acacatgtat ctgcaagaca ttgaccaaca aaatatggag ggaagaaaaa
      241 tgaaagatat ggatatcaag gccgtcttta taggcgacaa agctgaaaat ggaccagttt
      301 ataaaatgtt gttgaacaag atggtagatg aacatcttgg atggagagaa aattatattc
      361 cttcagatat gcctgcaatt agcgaaggag ataagctcac tccagattat ctagccaccc
      421 gcgaccatat gatagaggtc ttggatgaag tcagtcagcg attacgcgca ggatcgatcc
      481 catggcattc agcaggccgt tactggggac aaatgaatgc tgaaacattg atgcctgctt
      541 tattagctta caactatgcg atgctgtgga acccaaataa tgttgcattg gaatcttcta
      601 tggcaacttc gcaaatggaa gcagaagtag gacaggattt tgcttcatta ttcaatatga
      661 cagacggatg gggacacatt gcagcagatg gttctattgc taatcttgaa ggattatggt
      721 acgcccgttg catcaaatcg attcctttag ctgtcaagga agtgctgcct gaaaaagtga
      781 aaaaaatgtc tgagtgggaa cttttgaatt tatctgttga agaaattttg gaaatgaccg
      841 aaagctttac ggacgaagaa atggatgagg tcaaagcagc ttcttcccgt agcggaaaaa
      901 atatccaaag attagggaaa tggctggtcc ctcaaacaaa acactattct tggatgaagg
      961 cgttagatat ttgcggagtc ggcttagatc agatggttgc tatcccagtt caagaggatt
     1021 atcgaatgga tattaatgct ttggaaaaaa caattcgtga attagctgga caaaaaatcc
     1081 caattctagg tgtggttgct gtcgtaggaa cgacagaaga aggtcaagta gacagtgtag
     1141 ataaaatcgt ccaattaaga gaaaggttaa aagatgaagg gatctatttc tatttacatg
     1201 ttgatgccgc atatggcggc tatgcgcgtt cactgtttct aaacgaagca ggagaatttg
     1261 tgccgtatgc ttccttagct gaattctttg aagaacatca tgttttccac cactgcgtaa
     1321 cgattgacaa agaggtatat gaagggttca gagctatttc agaagcggat tctgtcacga
     1381 tagatcctca taagatgggc tatgtccctt atgctgctgg aggaatcgta atcaaacata
     1441 aaaatatgag aaatatcatt tcttatttcg cgccatatgt gtttgaaaaa tcagtaaagg
     1501 ctccagatat gttaggagct tatattttgg aaggatcaaa agctggagca accgccgccg
     1561 cagtctggac agcacatcgt gtattgccat tgaatgtgac cggatatggt caattgattg
     1621 gggcttcaat cgaggcagct caaagattca gagaatttct cgatcatctg acttttactg
     1681 ttaaaggaaa aacaatcgaa gtctatccat tgaatcatcc tgattttaat atggtcaatt
     1741 gggtattcaa agaacaaggc tgtacagatt tgaacgctat caatgaatta aatgaaaaaa
     1801 tgttcgatcg gtcttcttat atggatggcg atgtttatgg tgaacggttt attacttctc
     1861 atactacatt tacacaagaa gattacggtg attctccgat tcgttttgtt gaaagaatgg
     1921 gtctaacgaa agaagaatgg aaaaaagaac agaaaatcac tctgctacgt gcggctatca
     1981 tgactcctta tttgaatgat gatcgaattt tcaattttta tacaaaaaaa atcgccaaag
     2041 caatggaaaa gaaactaaac gaaatcatcc aatagaactg atttttgtgt gaaagaataa
     2101 caagagacat atggagaaca gacaattatt tgcttcatca aaacaattgt atttccccct
     2161 gtttttcata tattcctcgc caagatattc ttttttataa aacgttctat aagttggatc
     2221 gttcgtttca tgtatcaaac ccagttcact aagatcgttc aatggatgtt tcggtgatac
     2281 tgggttttgt actgaatcga tgaaaccttg tcatttctgt tcttgaacaa aaataaaaga
     2341 aaacaaaaac atatgtcgct attgaaagaa ggaataaaga tgaaagatac tggttcatca
     2401 aagcaaatca cgaccatgac ctttatcgga atgacctgcg cccttgtagc aagtgtacga
     2461 aatattccag atgtagcagc aacgagctgg acgatgattt tttacatgct ggttgcagta
     2521 ttattgtacg cttttccaat ctcattgatc tctggtgaat ttgctggaat gtttccccaa
     2581 aaaggtggtc cagaactttg ggtatcgaat gctctaggaa aaaaatgggg gttcgttgtc
     2641 tcatggctgt tatgggtcca aatgtttcct gggatggtga tggtcgcttc agcactcgct
     2701 ccgttgtttg gtaatattat cgataatgtg ccgcttggtt tgaacagcaa atttacatta
     2761 gttgtcattt tagtcgttta ttggatcatc acttttttga atttgaaatt tgatatggca
     2821 aaaatcggcg gaaaagtcgg tgtttggtta ggattgtacg tgcctttgac gatgatgctc
     2881 ttattagggt ttgctgcgtg gatcaaagtt gggatcgtac caaccggaac attaggcagt
     2941 ttttcatggg acaaattaat accagatact acgactgcat cctcatttgt ttattttgcg
     3001 ccgatcatgt ttatttttac tggtatcgaa atgtcctctg tgtatatcac tcgattggaa
     3061 aatccagtta aaacgtatat tcgcggagta tttgccgcgt taatttttat atttttcgtc
     3121 aatattttga atgctttagt tgtagcaaat gtcgtaccaa aaggacaaat ggaattgaat
     3181 aatatcgcgc aatctatttc gatttattgc caaattcttg gtttgccaca tcttattgtg
     3241 aatctgttta gtttacttgt cttcattggt gtagcagtcc aattatctgc ttgggcctca
     3301 ggaccagcaa agacagtgac agaaagtgct agaaaaggtg cgtatccacc aaaattcaat
     3361 ttttggaaga cgaatcaatt tgacgtatcc aaatccgtta ttttgactca gtctgtcatt
     3421 atctcaatct ttgcactttt ttatttgctg attcctggcg tcaatcaagc atttttgatg
     3481 ctcgtaaatt caactactgt tatctactgt atcgtctatg tcatcatggg gatcgcagtt
     3541 ttgagattgc gttatacaca tgcaaagttg aatcgaccat ttagaattgg aaaaaaagag
     3601 aaaaagagca atcttggagt ttggactgtc gttattgttt tatttgcggc aatcggattt
     3661 tctgtcggac tgacgatgaa agctggaaca tggatcaatc taattgcagt cacagccatc
     3721 agtgtcattt tgtttgtagt accactctgt attgaaaaaa taaagaaacc ttcttgggaa
     3781 caggaagtcg ccatgctgct tgaaaaagaa aaaggtgaac ttgatggaaa aaccggcaat
     3841 gaaacggact ctaactaatc gaaatatcca gttaatcgca ttaggtggcg caattggaac
     3901 aggattattt ttaggttcag caaaggcaat tgcatcagca gggccagctg ttattctcgt
     3961 ttatttgata tgcggtttgg ctatttatgg tttgatgcgg gccatgggtg atttattttt
     4021 agcaaatagt acatttaact ctatcagtga tttcgttgac tattatttag gcaaaaagtg
     4081 ggcatttttt gtcaattgga cttattggct ttgctggctt ggagctggac ttgcggaatt
     4141 aacagcaata ggattgtata tccatttctg gtttgtacat gtaccagctt tgataagcgg
     4201 aggtagcgca ctaatgatct taatggcagt caaccttgtt gcagtcaagt ggtttggtga
     4261 attggaatct tttttctcaa tcatcaagat cttggggatt ttccttttta tcttattggg
     4321 tataggattg agtgtctcag attttcatac ccagtctatc ggaatcaaag aaaattttgg
     4381 acagatcgat gaattactac cgtttggctt tgctggaatg gtagtctcat ttcctatggt
     4441 tttattcagt tttgctggaa ttgaaatgat cggcttaacc gttggcgaga caagtgatcc
     4501 gcaaaagaat ttgaaaaaag caatcaactg tctgccttgg cgtattctct ttttctatat
     4561 aggaacgatt ttgtcgattt tatttgttgt tccatggcat tgtttagatt taaaagaaag
     4621 tcctttagtg actatgctgc aagtgatcca ttgccaaggc ggagcagcaa ttgtcaatct
     4681 aatgattttg gttgcttctt tgtcctcagc aaatagtgca gtctacagta ctagcaggat
     4741 tttatatcaa acagcaaaag aaaaaagttt gccccattgg ttttgtgcgc tttctaagaa
     4801 aggcgtacct ttacacggta ttttaattac aggaatgtta ttttttgccg gtctttgtct
     4861 acattttttt gtagctgata gtcgattgtt ttttcagctt ttggcaggca tgacaacgag
     4921 ttgttttcta tttgtgtggt cttcgatctt gtgtgcacat gttcaattta ttaaagaaaa
     4981 gaaacggaac actacacaaa gatataaaga tcttgctttg ttggtgtttt ttacttcaat
     5041 tgctttcagc ttgttattcg aaagcacgac acgctttatt ccttttttat tggttatctg
     5101 gtttgtattc ttaaattttg tatataaaag aataagcaat agaaatatca gttaggccca
     5161 gatacatttt gcaaaacgaa ttatttcaga cccccctgaa ataatcgata cgcctgaaaa
     5221 aacttccctg cgtataataa cagtatcaat taaacaggag gggataggat gaactggtac
     5281 agattgacgg tagaagatgt attgaaaaac tatcaaacat caacagaagg cttgtcgcaa
     5341 gaagaacgac agaaacgcct gactgatcaa ggatataatg aaataaaagc aaggcagcag
     5401 acaaaacgat ggaaaaaaat agcaaagcat tttactgatc tgctgatgat cgtgttgatc
     5461 attgcctctt ttttgaaatt tgcttctagt gaatatattg aaggaagtat tattttattc
     5521 gtagtgatcg tcaatggact cgttagttac tggcaagaaa gaaaagcgga agaatcattg
     5581 aatggtttga agcagctgat gggacaagaa gcgatagtac tatcaaacgg gatgcaagaa
     5641 aaaatccctg cgaaagaact ggtaaaagga gatgtcattg tcttacgtgc cggagatgtc
     5701 gttccagcag atatccgatt aatcgaagca catgatttat tagtggaaga atcgattttg
     5761 acgggagaat cagaagcatc tgaaaaaagt catgccgttt tgacagaaga agaatcgatc
     5821 ggcgaccaga aaaatatggg tttttcagga acgcttgtac aatctggttc tgcagtcggt
     5881 gtcgttgtag aaacaggaaa cacgacagaa atcggtaaaa tcaatcaagc attgcaatcg
     5941 gtcgaacaac aaacgacgcc tctgattaaa aaaatcaacc gattaaacaa acaaattttt
     6001 caaggcattc tttgtttgag cctattctta gttattttta cgacatttcg atatggtatg
     6061 gattggcata tccttctttc ggctacgatt gctttggtgg tgtcaatggt acccgaagga
     6121 ttgccagctg ttttgaccat gattctatcc gttggagtac atgaaatggc aaaagaaaag
     6181 gcgatcatca aaggacttcc ttcggtagaa acattaggtt ctatgacagt gatctgttca
     6241 gataagacag gaacattgac aaaaaatgaa atgacagttg tggatgtagc tgcaaaagaa
     6301 gaagcctgcg tgttgtccat tatgaaaaac tgtcaggaat taaaaacaaa agaagaacag
     6361 aaaacagaaa atttaagtgg caatccgaca gaagttgcgc tgattcgaca cacagaaaca
     6421 gccaatctac ctttgaaaaa aatagaagca aagattccgt tcagttctga atacaaatat
     6481 atggcaacga tgcatgcgga ggaaaaagga gcggtgattt acgtcaaagg tgcaccagaa
     6541 gtattatttg ctaaatcaac tctttcttct gcagaacaag aagcatggag tcagacagct
     6601 gcagaatttg cgagaaaagg tcagcgtgtt ttaggatttg cttataaaaa agtagattcg
     6661 aaacaggaac tcacgcatga gactttaacc caattaactt ttgcagggat cgccggattg
     6721 atcgatccgc caaaagaaag tgcagtcaaa gctgtaaagg aatgccaaca agcagggatc
     6781 tcagtgaaaa tgattacagg ggatcacaaa gacacagcaa aagcgattgc ggaacaaatt
     6841 ggcttaaaac atactgccaa agttttagaa gggatcgatt tggatcttat gtctgatgaa
     6901 gagttgattc agcaagtgcc aatagtcgat gtctttgctc gaacgacacc agaacataaa
     6961 ctgcgaattg ttaaagcttt gcagaaaaat ggagaaatcg tcggaatgac cggagacggc
     7021 gtaaatgatg cacctgcttt gaaaagatcc gatgtaggaa ttgctatggg aatcaaaggt
     7081 agtgaagtaa gcaaacaggc agctgatatg gttttaggag atgataattt ccatacgatt
     7141 gcaaaagccg taaaagaggg ccggcggatt ttggataatt tgcaaaaaac catcaatttc
     7201 ttcttgccaa ctgcattagc tcaaggattg atcttgattt gggcacttat gctgaatcgc
     7261 ccgctacctt tgtccccagt acagatttta tgggtcaata tggtaacgac aatcacttta
     7321 tcttatgcac tagggttcga gtcgccagat ccagaaatca tgaagcgccc accaagagat
     7381 ccaaagcaag gaatcctatc tggttatcat ttgtttcgga tcatctatgt ctccctactg
     7441 atcatggttc ctgcctattt attggctatg cagtttgaag ggcaaagctt acagcaaacc
     7501 attcttctgc aaaatatcgt cttagctcaa gctgtttaca tgatcaattg cagagagttg
     7561 tccaagccgt cattgaacca aggtcttttg caaaacaaag cgctttttct ttcattaggt
     7621 atccttgctt tattgcaagg gatggttatt tttctaccag tcggccagca gctgatcggt
     7681 acagtaagtt taacgatgac acaacagtta ttgattggag caaatgtgat tcttctattc
     7741 ttagttgtag aaatagaaaa agggatcatg aacaaactgt ttcgcaaaaa agaaaaaaca
     7801 gctagagaat acagtcgcta gccgttacta taaaaaagtt ttgaaagtga gttcagtcaa
     7861 acgattgagc tcatttttcg ttggaataga agaaatgtat acaataggga tatctagaga
     7921 atctagaggg aaattggaaa tcaagatatc tgttttttca aaagaaatag tggaaagatc
     7981 ggttagttcg ataggtaaga agcagacacg ttttcctaag taatctttta attcctgctg
     8041 caagaacgcc ttccatgaag gttctccttg gaaaataaaa tagacattga acaaattcgg
     8101 tttgatagaa aaaatcgttt gttggatcag tagagacaag agttggagaa agtaatttgg
     8161 ttcattgatt tctatttctt tttttagatc gttcagtaaa ttctctttct gttgatacaa
     8221 gtgtgcatat tgctgatcat atgtttctaa aaaacgatgg tattgacggt ccaatgcttc
     8281 aatcaatatc ggcgattcat gatattttaa tatgaggaga gccaagttat cagtcagaga
     8341 tgtttttttt agttgatcaa gagacagctc tgaagctagt tcctctacaa acgtttcgag
     8401 gacctctaaa aatggagaat gatgcaggca gcgagcggtt tcttgtccat agttattgtt
     8461 gtaattccaa ctttccagaa agcagaaaga agcaaaagaa agctcagtgt ctttaagata
     8521 aataccttcc gttaaataca attttttcaa tgcagagtca tacagggagg agagtgtgct
     8581 gttggtatgg atagaagaat ctaatcggca attcgctttt atacggatcg tattgataaa
     8641 gaaccaacat gttccgaaaa tctcttctgt ttcaaccgct agttcagttt gactgaggtt
     8701 tcttaaaaat tgaaaataat gggattcatg gatcgaataa tcctcgaaaa aatagttgtt
     8761 atgagtgaaa ggaatcagca atcgatgata aaaagtacga atcgcacgct cttttccaag
     8821 aatacaagct gtatatttcg tcaaatcgat ctgcaagtcg taacttgcta aacgctgatt
     8881 gattttagtc acatgtcttt tcaatgtccc ataagaaagc ccatggtcgg ctagaaaatc
     8941 agggacagca actttttttg taaaaagcag atttttcagc agttcgacac tgactgattg
     9001 tttgaaaaaa atctcccaaa ggtcatttgt taataaattt tgctggtttt gcaaactgat
     9061 tcctgcttga tcgctgatga tgtcccagcc ttcgggcagc tgagagcgaa tagattgaag
     9121 gtcagaaaaa atcgttcgtt cggttgtccc tatgatttca gctaattcat ttacggttat
     9181 ctttgtatga ttcagcaatt gttctactaa agaaacatga cgtcgaatat ctttttcggt
     9241 aatgattttt ttcattagtg aatacatcaa tcagactccc ttctattcta agaatacgga
     9301 gaaaataaac ggtggtcaag tttcaactga tttttcaatc attgtttatt tttctggagc
     9361 atttactgat gataagaatt tcatcatgaa cttgaaaaat gataccacta aaatcaaaaa
     9421 ataggtgggc aaacaataat aaacatttta atagtagatg gtagcaagac caacacaggc
     9481 ggattgaaca tttaagagtt gataaaaaag aaaaatatcg aagtatttga ttagcaacta
     9541 cttgtattca tttttctatt cttacgtggt atctttgaat aaagcgaatc aataagaaag
     9601 agatggaagc agatgctaac gaaaaaagaa ctaatcgatc acatcaatga aacatacaat
     9661 ttgtctccag aatatatttt taaaaaattt cctgattatt gcgtattcag acatcaaaaa
     9721 agcaaaaaat ggtttggggt cctgatgtct gttccagaag aaaaaatcta tgatgatgca
     9781 agtgaccata ggatcatcaa tattcttgat ttgaaggtcg atcctgaact tggcggaatt
     9841 ttgcgaggaa aagaggggta ttttccagcc tatcacatga ataaagaaca ttggatctct
     9901 attgatctac gtaaaataga aaagctagat gaactagcag atttgattga tggaagtttt
     9961 aaatcaacgc aatgaatgtc taggacaata gagaagaggt tgtgacagaa atgtttagct
    10021 tcaagaaata agaaggaatt tacgaaaatt gcttctcaaa tttttgggaa atttcggctt
    10081 attttctaag aaattacttc tggaacaccg tttgttcgaa aaaagagctg cgacaagaat
    10141 tttgtcccag ctcttttttt tttattttcc taaataaaat tcccgcatcg tttttgcaat
    10201 cattttgatc aaaggatgat cttctgtgaa gctagaagat aaataaattg ttcgtgtcaa
    10261 gggcaaatct ttgatttccc gcaagacgaa aggatcattt ttatcgtaag accaagaaac
    10321 ttttgggaaa aagctaacag ctaatccttg cttgacaata gaacgtaatg tcgcaggatc
    10381 atctgtcgtc gaactgatcg tcaattcgat acttttcgct tcaagtgctg catccagtgt
    10441 atgacggaag gcgttgctct ttgacagcat caagatgggg gtgcgttgga tatcttctaa
    10501 ttgcagatgt tcttttttag caagaggatg tttcttagga atggcgacat aaacaggttc
    10561 tttcatgata ggaatattgg tcatgccagg cttcatctcc gaagtgatgt acagtacagg
    10621 atcggtaata tcatctggta aatgatgttg catgatcgtt aattggatat caggatgctt
    10681 ttccataaaa attcggacca agttcggaat agaaacagag gctacctcta aatacaatat
    10741 gacggaagta acgatcttct cattgaccga agacaataat ttgattcctt cttctaatgt
    10801 gtctaatgtc ttgactgcat atggataaaa ttcttctgca taaacggtcg tctcaatgtt
    10861 tctgccgcta tggtaaaaca aagggacacc taattcgttt tccaaatgtt tgatcgcacg
    10921 gctcaaggca ggttgagaaa taaataattc tttggctgct tgggtaacat tttttgtttc
    10981 gaatactttt ttaaaatact gcagatgaag caatttcata attatccatc ccatccatac
    11041 ctttttgtta tggttttttc tttttttggc attttttatt atagcagaaa ttgactaaga
    11101 tagcagtgga aaaagtaagt gctgaggagg cgaggctcat gagtacaggt ttcttatttc
    11161 ctggacaagg cagtcagaag ttagggatgt tagcggatat ccctgaaatg tacatggaac
    11221 gtttagaaaa tgtgacgggg tatcggttag ttgataaaga agaaaactat caagatacgg
    11281 tttttatcca gctagcatta ctgacaaaag cagtatttta tttagatgaa atggaaaaaa
    11341 acggtattca gcctgatatt gcagcaggac attcgattgg tgctttttgt gcagctgttg
    11401 cttgtcgatc tttgagattt gagaatgcag cggaactagt ctatcaccgc gcagtcttaa
    11461 tgaaagaagc ttatccaagc ggttatgcaa tgggcgtagt agtaggtgtg acgagaagcg
    11521 aagctgagga aattgtagca aaaacatttg atcctgacca tccagtttat ctgtcaaatg
    11581 aaaattgtcc gatgcaacat acgttatctg ggagtatcga aggtttatcc gttacgttgg
    11641 cagaagccaa acggcaccaa gcgcaaacag cgaagctgct gaaagtccct gtgccatctc
    11701 actgtctctt gatgaatgat acggtcaaga aattcaagcc gtatatgcag tctgtccatc
    11761 tatctgatgc tagatgtttg tatataaaaa atacggatgg aagagccact caggacgcag
    11821 aagagattcg taaagatttg ttggaaaacc tagctttccc tgtgcagtgg aaccaaatga
    11881 tggacttggc aaaagaatta ggaatggatc tgacgattga atttcccccc ggcaatactt
    11941 tgacaaagct gattcataca aaatttggtg tagataaagg gatccgtacg atcaatctag
    12001 accaacatgg tattgatgac gccacgtttc tatatcaaaa atggagatga ctaattatgg
    12061 aaaaaccaaa ccgcaactgg gcttctaaac gcgaaaaaaa agcgcagcga ttggaaaaaa
    12121 tcagcaactt gctcaatggc aagtatgtag aaactgaaaa aatcgtagaa gtaatggaaa
    12181 aactgattgc tcccggcgac aaagttgtat tagaaggcga caatcaaaaa caagcaagtt
    12241 ttctttcaga aagtcttgaa aaagtagatg cagaaaaagt gaatgatctg catttgatca
    12301 tgtctagtat ctcacgacca gaacatttga atatttttga aaaaggaatc gcaagcaaga
    12361 tcgatttctc ttatgctggt gcacaaagct tgcgtgtcgc ccaaatgatt gaagacggca
    12421 caatgaaatt aggtgatatc catacttatt tggaacttta tggacgctta ttcatcgatt
    12481 tgattccaaa tgttgtttta gtagctgcag ataaagcaga taaagacggc aacttgtaca
    12541 caggtttcaa tacggaagaa acaccaacaa tcatcgaagc tgcagctttc aaagatggta
    12601 tcgtgatcgt tcaagttaac gaagttgtcg attctttacc acgtgtagat atccctggtg
    12661 actgggtaga cgttgtcgtg aaagcagata aaccttatca attagaagct ttattcacac
    12721 gagatcctca aaacatcaca gaattgcaaa ttctaatggc aatgatggca atcaaaggaa
    12781 tctacgcaga acacggcgta caatctttga atcacggtat tggattcaat acagcagcta
    12841 tcgaattgtt actgccaact tatggtgaat cactagggtt aaaagggaaa atcgcgaaga
    12901 actgggtatt gaacccacat ccaacgatga ttccagccat cgaatcaggt tgggttgaaa
    12961 gcatccatag ttttggtggc gaagttggta tggaaaaata tattgctgct cgcccagatg
    13021 tcttttttgt cggtaaagac ggcacgatgc gcagtaaccg aacattatca caagctgcag
    13081 gacaatatgc catcgatatg tttatcggtt caaccttgca attagattac gaaggaaact
    13141 cttctaccgt aacaaaagga cgtttatctg gttttggcgg tgcgcctaac atgggacata
    13201 atcctggcgg tcgtcgtcat tctacaccag catggcaatc acttcgcgac aatgatgatc
    13261 cattagggaa aggccaaaaa ttagttgttc aaatggttga aacatatggt tcaaataaaa
    13321 aaccagtctt tgtcgaagaa ttagatgcac tagctgttca aaaacaagca ggcttagcca
    13381 atgcaatgca ccagtgatga tttatagcga agatactaca catatcgtga cagaagaagg
    13441 aattgcttat ttgtacaaag caaaaacaaa agaagaacga caagcagcaa ttgcagcaat
    13501 tggcggtgtt acacctcttg gaatgactag cacgaaagaa tcactagata gcttgcgtgc
    13561 agccggcatc gtgaaattac cagaagatct aggcatcaaa cgtagtgaag cgaaacgttc
    13621 attattagct gcacaaacca tcgatgattt gatcgaatgg tcagatggcc tgtatgaacc
    13681 accaatgaaa ttcagaactt ggtcataaaa ttggcgagaa aaaccttttg tcgccattta
    13741 cccagatgga agaagaaaga cgaataagca gaacgtgtat gctctgtttt acaaaaaggg
    13801 gagagatcca tggaacaatt agcttataaa tatccaacga aacagccgat caataagaaa
    13861 atccatgtag gagtggtcgg ttcaggagat ttggaaatct tgatgtttcc aacagaaaaa
    13921 acagaatcta ctgtaaaaat ttgtacagga agcgatggct tcggcgaagt atggaacaat
    13981 gtattaacga gattttttga tcgttatcca atttcagcag atatcgtgat caatgacttc
    14041 ggtgctacac caggagttgt gtacctacga ttgacgcaag caatggagga actttcagat
    14101 gaaaaatagt tttgtagaat taaacgccag agaacgagtg aacgctctac ttgacaatgg
    14161 acgtgaattg attgatccat tcgatcaaat catcgcgcca agtttaccag cacaaggaat
    14221 cgtccctgaa agtgatgacg ggatcgttgt cagccgtggc acgatcaatg gcaaacaagc
    14281 agtagtaatc gctatggaag gaaaattcca aggtggcggt atcggtgaag tttccggtgc
    14341 aaaaatcgtt gctgcacttg aacatgtgtt agaagaaaat aaaaaaggaa atgagatctt
    14401 tccagtcatt gtactagata caggtggtgt tcgtttgcaa gaagcaaact acggactgtt
    14461 atctatttca gaaatcggaa atgtaatcat tgctctaaaa caatatgtac cagtcatcgg
    14521 tttgattcca ggacgtgtag gatcatttgg cggtatgtcg attacttcag cattaatgag
    14581 ttatttaatc gcaacaaaaa aagcgagagt cggtttgaat ggaccagaag ttattgaaca
    14641 agaagcaggc gttcgagaat ttgattctag tgacaaagac ttgatttgga atacattagg
    14701 atctcgtcaa cgggtagaat caaaaatcat tgatgaatta gtgactgata gtgtagaagc
    14761 aatcaaagct gctgtcatcg cagcaatgga cgaacgaaaa gacacgcatc gcagtgaaag
    14821 ctcagcattt tatttatcat tacttgattc tttagatctt tcaaaaccaa tggccattga
    14881 cgattacaat gaagcaattg ctaaccatca agcaaaatca attgacttac cagaagtagc
    14941 agaaggaaat gaaccagctg cagcaagtgt tggttacgat tggtttgcgg cactaactgg
    15001 catgaaaaat ccaacttctt ctattagtac tgtgtattat ggtaaatcaa gtaaattcat
    15061 agaagaagcc atcgtgacat caatcgtacc tgatcctaaa aacccgatgt accgtgtacg
    15121 aaacggtgaa gttggtttag ttgaaggttt ccgcatggga caaattttgg atcacgttta
    15181 cgaagaagat aaagacaaaa aagtgaaacg gccaatcatc gttgtgatcg atgtaccaag
    15241 ccaagcatac ggctataacg aagaattgat cggtatccat gtggctttgg ctaatagtgc
    15301 tgctgcttat gcaaaactcc gtcaagctgg acatccagtt attggattga ttgcaggaaa
    15361 tgctatttca ggtgcatttt tagcacatgg attacaatca agccgcttga ttgctttaaa
    15421 caataaagaa attaccgtcc aagcgatgtc taaagaaagt gctgcacgtg tgacaaaacg
    15481 ttcggttgct gaaatcgaag cagctgctga aaaagttcca gctatcgctt acgatatcaa
    15541 taattataca aaactaggag cgatctatca tttcctagaa gatatcaccg accaagctgc
    15601 ttcagaacaa aatgtcagca aagtcatcga agcaatcaat gaagccattc aagatgtccg
    15661 caatacaaat gacacgatgc tgacaaatcg atacacaaac gaaacagcca aaacattcgg
    15721 acgtgtcgaa acaaataaag tccgtgccaa aatggacgaa gaatgggatg cataaaaaat
    15781 gattcaagca catgatattg tcctttttga taaacatgaa atagaagggt tcaccttgcc
    15841 tgaatgggtc gttctaactg aaaagatgtt cggaacagtt cgtcgtgcgt cgacggatca
    15901 cgcagaaata ctaccgattg gtttacgtgg acctgcccgc aatcagagat ggtcaacaga
    15961 aatctcaaaa catgccgtca aacaagtcat tcatccgtgg gacatcatcg aacaagaatc
    16021 gtatagacaa gagaaaatca tggattatcc tgtttatcag caatttcatg ctgctagaaa
    16081 catattatca ggatgtaaat ggggaatcgg cggcagctta ggatttgaat tatcgactgg
    16141 gattccggca gtaaaagaaa ccagtgattt tgatttgctg ctgtacgcaa attcaccgat
    16201 tgaattgcca attcaggtaa ttcaatcgca tcctgctttt tttgagcaat tcgataccca
    16261 agtagtgact cggcaaggcg gtttttcact aaaagagtat ttgagagagc cagagaaaaa
    16321 aattctattg aaaacagtcg aaggacctaa attaaccaaa gaactttggt aataaagaaa
    16381 aagagagaga aagagatgtt tgaaaacaaa aaaatcaaaa tcaacgtaag tgacttccag
    16441 aaagtatcag ctgtaacggc tgttatgatg caaacgatat tgagctttgc tttggcaaac
    16501 agccacagcg caacaaatac agcttttctt ggtacctttt acgtcatcgt taaatacaca
    16561 gcaccaatgt ttatttttgc aattgtttat aatatggtaa aaacaagtca gcatttgtct
    16621 tattgggaat ttttgaaaga aaagtttttt gaactagtcg ttccgtatct tctatggacg
    16681 acggcttatc tttggctatt tccagcagtc caacaacaaa ctccgtatac ggatactgca
    16741 agctttttac tgaaatatgt cacaggtgat ggagcgcctc atttatggta taccgttatg
    16801 atgctgcaga tccaattgtt catgccgttt tttgtttggc tcggttacaa agtatttgct
    16861 aataaaaaat atgtatggcc agtattgatt ggttcaacga ttttatatgt cgcatggtat
    16921 ctgttctatc aaacacaagt attgactggg ccttatcatg acagctggta cttattagat
    16981 cggtttgttg ctagtttcat gatttatggg gtagccgcat ttgtttatca cgaaaaagtc
    17041 tatcaatatt tggacagagt acgttatctt ttcttacctg ttgccttagt tatcgcgttc
    17101 ttttctgtcc gttcattgct cgcacatcca ggagacttga gctttgcgaa tgcaccttac
    17161 ttgaatacga tccaaagttt atacagttta gtcatcatct ttgctgtctt cataggcgct
    17221 tccaaaatga ttgtaaacga ttcgccaaaa ttgccattgt tcaaatggtt gtctgtttat
    17281 gcttatcgta cgtatttagc caatgtattt gttttccaag tactgttatt attgtttaaa
    17341 gatagttggc tgcagttacc ttcaggagtc atgattcttg tagcttatct aatgacggca
    17401 agttgtgcat ttgctttaag ctggttactt catatcattt gggtagctat caaaaaaggt
    17461 ttcagcaagt aatagtagga agaaggagtg agtgtcatga taaatgacga acgaaaaaaa
    17521 gcagtgactc agcaactcgc tgcgttggta gaggaagcat tgatcgcaga agtaacatta
    17581 agtccaaaac ctggattagt ggatgcactg gatgcgggtg cacattccga tatggactat
    17641 gctttatttc ttaagtctgc taagacgcta actccttttt ttgaagaaat ggctcaaata
    17701 gcatggcatc atgctattga tcaggagtta agagaaagaa ttgctgagat tgggcgaaaa
    17761 gcggaaaagg caatgctgat tgctacaaat ggcatcaata cccataaagg agcgatttgg
    17821 gcaatggggc tattgatcag tgtcattgca caaaaaatca gtatggaaca gcctttttct
    17881 ttatcgaaaa tacttgatga tgtcagcaag cttgctgctt ttcctgatac aaaatatcgt
    17941 cataaaaaaa ccactcatgg tcatagggtg aagcaaaaat atggcgtaaa tggtgcatat
    18001 gaagaagcga tattagggta tccgcatatc aggcttgcaa ttcaagcagc taaggagcta
    18061 gataatgatt catatgtaca gaaacaaatg catatgttgt tagttttgat gagttcgcta
    18121 gatgacacat gcgtattgca tcgcagtgat cagaaaacgt tagtggaaat gcaaaaactt
    18181 gctcatgctg ctagtcaaag tgctttacca aatcgtccat ttacagcact gttgcacttt
    18241 tgtcaggaaa accaaatctc tcctggagga agcgcagatc tcttgtctgc aagcttgttc
    18301 atgctgaaga tagaggaatt atggactgcc gacacataca tttgtccgat gttactcaat
    18361 gactaaataa gacaaaattg ctaaaaaaac agtttatgca tctggttatt gactcttcct
    18421 tttagcaagg ctatcataaa aatagttttt tgtaataaaa gataacgagg aagacaatga
    18481 aaaaaatcaa ttatttagca atcattttgt tagtgggcct atgtttgttt gctctgccga
    18541 cccaaggatt cgctgtcgcg ggtggtcatg gtggaggtac atctagcggt cgaagttcgg
    18601 gcagaggttc atttggtggt cgttcatatg ggggcagtta ttttggcagt cgttcttacg
    18661 gcagagattc ttatgggtat tatggtccag gtacaggtcc ttctagttat gagcaaacgg
    18721 gatttcttgc attagctgtt ttgtcagtag gaggggtttc tgctttttct atcgtcaaga
    18781 ataaaagaca aaagaaaagg ctgaaagcgg agatattgtc ccgcatgtca ggaacggcta
    18841 aagaaaagaa agcacagttt ctggaaatcc aaaagatatt catgaagatc caatccgctt
    18901 gggaagaaga aactcctgaa aaagccagaa gctgttatac agacaagctg tttgccgaac
    18961 atcagaaagt tctgcaaaaa aacaaagcag acggtgtaag aaatcatact aaaaaaataa
    19021 ctattcagga agtaggaaat tatcgacaga tccacgaaaa tagttttgcc atccgaattg
    19081 atttcagatg ctgtgattat tcagtcgatc gaaaagatgg gcgattgatc tcaggaagta
    19141 agacacaacg acagtatttt tctcagatat ggtattttga tttttctgag aaagaaaaaa
    19201 gatggcaagc cgattttatt cagccgatta tgctggattg acaggaaata tttatgaacg
    19261 gagttgtgac aaaagccttg tcgcagctcc tttttctgaa taaacggtgt gccagagata
    19321 gcctatcggc aataaggcac gattttgtaa aatatgggaa gcgatttttc aaaatcgctt
    19381 cttatcgctc aaggctgaac atctctgtca caacctctcc actaaatggt gtttaaaagc
    19441 ttgcatctgc gataataaga acaaccaaac gaaaaatagg aagcactatt ttagtttagt
    19501 tgttcttatt acttgatgca ggacagcttt ttcacaacct cttttttgta tagatgggca
    19561 aaatttttgt aataataggc gtatgtcttt ttcgttgagg agatgaaaaa gcatatggct
    19621 tattttttgt ttgctttgct tactttcttt ccacttcaag tggcagaaat atctactttt
    19681 tattatgtaa gcggaaccat atacttgaaa tcagaaggag atcatttcta tgaaaacaag
    19741 aacaattgaa atacttcaca gaatcatgac agcccaatat ccagtaaaag caaaacatct
    19801 agcaagtgag ttcaatgtca gtcagcggac gatccgtcag gaagttcttg aagcgaatat
    19861 ctggttgagc agtaaaaagc ttccggagat ccgtacaatt cgcaacaaag gttttttgtt
    19921 gaagctggaa aaggaaaagc aagctcgatt agaaactgaa ataaaagatg tgaaagacgg
    19981 ctttttgaat cggcgcgaac gtatttttga tctagtttta gccgttgctt acagccgaat
    20041 cccgatccat ctaaacaaaa aagaagaaga atttcaaatc tctaaaagta cattagatga
    20101 agatatgaga cggttacgag caacactggt cagttatggg atagagatcg tcagttatgg
    20161 aaagcaagga ctggtttata aaggttcaga acgatctatc cgtacgatga tctatgattt
    20221 tatcaatcag aatctgggaa tgaccgactt ctttatgaat caagatgcac aattaacacc
    20281 ggctcaaaaa atctttctcc attattttcc tttggtggaa atcaaaaaaa tacaggagat
    20341 ctattccaat aagttgttga aacaagaaga tgatatttat aaaaaacaaa tcctgctttt
    20401 tacattgatc tggatccagc gaatcaggaa acatgaattt atctctgcca ttaattggaa
    20461 aaatacagaa ataaaagaca gtagcttttc tgcatttatt gaccacatga tcgaggaatt
    20521 tcaattggac aatgtcccgc aagtagaaag aaattatctt cgtttcacta ttgagacgtt
    20581 caatgctaga gatatctcca attcaatcga gtgggtacaa gctcaattac taacgatcca
    20641 attgatccaa tttgtagaga acgagacaca cgtacctttc catctaaagg aagaagcttt
    20701 attcgaaaat ctctataagc acatggctgc actgattgta agaatcaaga atcatatcca
    20761 agtcatgaat ccattgaaag aaaacatccg aacaaattat ttcccaatct accgcgcagt
    20821 cactcatttt gttcctacga tcgaagaagt aattggtggc aagatactgg aggacgagat
    20881 agcttttctt gtcatccact tttcgacgat cgccagtgta atcaagcgag acttgaatta
    20941 tgtttataaa tcagtcgttg tctgtaacca tgggatggct actggaaatc tgttagcaga
    21001 gaatctgaaa gaaaagtttc ctcagatcga gattactgca gttttaagtt caaaagaaat
    21061 cgaattagta gataaattgg atgtcaatct ggttttcagc acctttcaat tgaattatcg
    21121 aaataagccg cttttagttg tcgatccgat tttaactgag acgaatatct tggttataga
    21181 agactttttg aaaagaaatg aagaactaca gcggttagag cctaacagtc atgaatcgac
    21241 ggagttattc actcaaataa tagatgtcat gaaagaaagt ggtggccgag tagatcgaat
    21301 catctatggc aagttagaaa aattattcga aacaaacaat ttagaaatca acaaaaggga
    21361 gatccagcct atgttaaaag atattttgac ggacagcaat atcatgatca aagaaaaagc
    21421 cactacttgg gaggaatcga ttgaactagc tgctagacca ttggttaaag aaaacattgt
    21481 cgaagagcgc tatgtcgaag cgatgattcg tgcagtgaat gattacggac cgtatatcgt
    21541 gattggcaaa cacatggcac tagcccatgc ccgaccagaa gatggggtca ataaattagg
    21601 agtcagcgta gctacgattg agcaaccaat cgatttcggt aatcctgaaa tggatccagt
    21661 aaagattatt ttttgtttgg cagcagtaga ctcctactcg catctgaaca tcatgaaaga
    21721 actaattgaa ttgatcaaca acgaagagaa gttggatcga ttgattgaat gcacaaatat
    21781 cgaagatttc aaacaactgc ttttttcagt agttgagaaa taataaatga tacgggggag
    21841 aacaaagatg aacaaattga acattttatt cgtatgcggt gcaggtttag gaagtagttt
    21901 tgcagcacag atgtctgctg aggatgtttt gaacaaatta ggagtagaag ccaaactgga
    21961 ccattgtgat atttcttccg cagtttccat gaaaccagat gtgatcatca cagcagaaaa
    22021 ttttcgttcg cagttcgaaa agttcgcagt tgatcctaaa acggcgatga tctatttgaa
    22081 aaatattgtt tcaaaaaatg aaattgaaga aaaaatcacc ccagttctgc aagataaggg
    22141 agttatctaa atacaaaaag aaaagagggt aaaaa
//
LOCUS       DAZBTF010000047        21845 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000047 DAZBTF010000000
VERSION     DAZBTF010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.047"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            27..581
                     /locus_tag="ACHGNS_001817"
     CDS             27..581
                     /locus_tag="ACHGNS_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN6937757.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(754..1191)
                     /locus_tag="ACHGNS_001818"
     CDS             complement(754..1191)
                     /locus_tag="ACHGNS_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN6937758.1"
                     /translation="MFSSEENKPLYLDYVNHNLKIIIKENNLKHITLHGFRHTHCSLL
                     FESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFMSSTEEKQGSLQK
                     IKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1239..2186
                     /locus_tag="ACHGNS_001819"
                     /pseudo
     CDS             1239..2186
                     /locus_tag="ACHGNS_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            2457..2892
                     /locus_tag="ACHGNS_001820"
                     /pseudo
     CDS             2457..2892
                     /locus_tag="ACHGNS_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            2908..3741
                     /locus_tag="ACHGNS_001821"
     CDS             2908..3741
                     /locus_tag="ACHGNS_001821"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN6937759.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            3986..5011
                     /locus_tag="ACHGNS_001822"
     CDS             3986..5011
                     /locus_tag="ACHGNS_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN6937760.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5149..5274
                     /locus_tag="ACHGNS_001823"
     CDS             5149..5274
                     /locus_tag="ACHGNS_001823"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937761.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            5299..6414
                     /locus_tag="ACHGNS_001824"
                     /pseudo
     CDS             5299..6414
                     /locus_tag="ACHGNS_001824"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            6497..7393
                     /locus_tag="ACHGNS_001825"
                     /pseudo
     CDS             6497..7393
                     /locus_tag="ACHGNS_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            7409..8233
                     /locus_tag="ACHGNS_001826"
     CDS             7409..8233
                     /locus_tag="ACHGNS_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6937762.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8265..9575
                     /locus_tag="ACHGNS_001827"
     CDS             8265..9575
                     /locus_tag="ACHGNS_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFN6937763.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9723..10982
                     /locus_tag="ACHGNS_001828"
     CDS             9723..10982
                     /locus_tag="ACHGNS_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFN6937764.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            10992..11864
                     /locus_tag="ACHGNS_001829"
     CDS             10992..11864
                     /locus_tag="ACHGNS_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6937765.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            11876..12706
                     /locus_tag="ACHGNS_001830"
     CDS             11876..12706
                     /locus_tag="ACHGNS_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6937766.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12746..14929
                     /locus_tag="ACHGNS_001831"
     CDS             12746..14929
                     /locus_tag="ACHGNS_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFN6937767.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            14990..16210
                     /locus_tag="ACHGNS_001832"
     CDS             14990..16210
                     /locus_tag="ACHGNS_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFN6937768.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16207..17667
                     /gene="gtfA"
                     /locus_tag="ACHGNS_001833"
     CDS             16207..17667
                     /gene="gtfA"
                     /locus_tag="ACHGNS_001833"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFN6937769.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17744..18850
                     /locus_tag="ACHGNS_001834"
                     /pseudo
     CDS             17744..18850
                     /locus_tag="ACHGNS_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19172..19381
                     /locus_tag="ACHGNS_001835"
     CDS             19172..19381
                     /locus_tag="ACHGNS_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFN6937770.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMASKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19374..19523
                     /locus_tag="ACHGNS_001836"
     CDS             19374..19523
                     /locus_tag="ACHGNS_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFN6937771.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            19677..20342
                     /locus_tag="ACHGNS_001837"
     CDS             19677..20342
                     /locus_tag="ACHGNS_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN6937772.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(20626..21711)
                     /gene="xerS"
                     /locus_tag="ACHGNS_001838"
     CDS             complement(20626..21711)
                     /gene="xerS"
                     /locus_tag="ACHGNS_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFN6937773.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT         7421 a         3292 c         4209 g         6923 t
ORIGIN      
        1 gaatattttt aagaagggaa acaatagtga gctataatgt acaaccgtta aggacaaatg
       61 aagagataca agattttcag ttttggctaa gaagaacaag taatccggaa agagatagtt
      121 ttttattttt gtttggaatc aataatgggt tacgtatgtc agatattata ggattaaaag
      181 ttggagatat acgaggcaaa agtaagccta ttattgttga acataaaaca ggtaagcgaa
      241 aaccgatttt tatagataat ttgagagaag aaattttatt atatactgaa ggtaaagaag
      301 aaaatgattg gttatttccg agtcggcaac aaggtcgaca tataacaagg gatcgtgtct
      361 atcagatata tgcagatata gcagaaaaat taggacgaga tgatatagga acacatacat
      421 taagaaaaac ttttgggtat cactactaca agaaaactag agacattgct accttaatgt
      481 ttatttttaa tcatagctcg caagcaatta caaaaaggta cattggtata actgaagatg
      541 aaataggagc atcacttcgt ggatttaaac taggtgtata atttaaaaat ttttataaac
      601 agtaaagaca aagattaaaa gctattttta tctagaacga ttttatttca taaagttcgc
      661 ggaataaaag ctggggttac tggtacttgg tataggttcg tgttatataa actaagtaat
      721 taaataacat ttttaggttg gaaaaggatg aaattaaaaa aagagaatca atacggtctc
      781 aaaaacgcaa acgctgcgga ataagaaaaa tactgataac tcaacgttta gcagactatc
      841 taatagggat agtctgcttt tttcgttttt gaccatactt ttgatcttct gcaaacttcc
      901 ttgcttttcc tctgtagaac tcatgaaatc tgcaaaacga attgccgttt cctctgtctg
      961 ctttttcgcg atatgggtgt atatatcatc catagtggtt ttaatatctg tatgtccaag
     1021 tcgaacatac acttctttga cagacgcacc agattcaaag agcagactac aatgggtatg
     1081 acgaaaacca tgaagtgtga tatgttttaa gttattctct ttaatgatga ttttcaaatt
     1141 gtgatttaca tagtctaaat aaaggggctt attttcttct gatgaaaaca aaagttgttc
     1201 accatctttt ataggtttga ttctcatttt tagatattat gcccgcacgt attcatcaca
     1261 tttctattgt gaatcgcttt attcgaccga catttgactt ttatcataat atactcggtt
     1321 taaaattact aatgaaaaca attaatcaag acgatcatac catgtatcac ctgttttttt
     1381 cagataatca ccaccgtgtg ggaactgaac tgactttttt tgaagtacaa gaagggaaca
     1441 accagttttt tggaaccaat acgattgaac gaacgatact aaaagttcct tccgaagcat
     1501 cattgcattt ttgggaaata tattttgaaa cacaaggcgt ttgccattat ggaatcgaat
     1561 cattttctgg acgacctatc ttacgttttg aagggccaga tgcaacacga ttagctttag
     1621 ttcccttacg cgaatttgaa gatattgatg attatttccc gtatgtcaca gaccagatcc
     1681 ctacagagca tgccatttta ggaattgatg ccattcaact acgcgtacaa tattccgatg
     1741 cgactgaacg agagttattg tcggtaggct ggtagcacaa agaatacgtc agtttctttt
     1801 cgggtcatca acgagtgaca atcttagaaa atcaagacac tcagttctat caagaagttc
     1861 agattattca agatcgtgag aatccagttg ccattgaagg gattggtagc gtccatcatg
     1921 ttgcgtttgg cgtagaaaat aaatctgatt tacaacgcat cgacaaacaa ttacaagagc
     1981 gcaattttat aaattcagga ataaaagatc gtgaattttt tgtatcactt tattatcgtg
     2041 acccaaatca attattgatt gaaattgcta caggggaagg aaacctagat gcaaaagcgt
     2101 atgaaaatca aagtccacgc tttgaagaaa ttcctttatt tttaccgcag tacttaaatt
     2161 tcattcgtga acaagtggag caataattag ctaatcaaat ccatgacgaa gagtaagagg
     2221 ttgactcata agtcaaaaaa taaagaaaaa cgcatcaaat ccattctaaa gaatgttgat
     2281 ttgatgcggt tcctattttt ataaactatc attttcgact aataaaacta cttatgagtc
     2341 agcctctctt tttttcttga tttttatacc tctttctagg taaactaaac atctgccaaa
     2401 aataccgtgt ctcattgaaa cattctcaac tatttcaaaa taaaggggag taccaaatga
     2461 acgagtatat tgaaattatc cacgcaagag aaacaactta aaaatgtcag tctcaaaatt
     2521 cccaaaaata aaattagtat ttttaccagc gtatctggtt caggaaaatc atcgattgtt
     2581 tttgatacta ttacccaaga agctggtcgt caactgaatg acacctatga tagttttacc
     2641 cgtttatttt tacctaaata cagtcgtcca gaggttgatg agattgataa tctttctacg
     2701 gcgattgtgg tcaatcaaaa gaagattggt ggcagtgcgc gctcaacgtt agggacgatt
     2761 actaacatca atgcgttatt tcgtgtgttg ttttcatgtt ttgcaacacc tagtttagga
     2821 tatgcaaatg cttattcatt taatgatcct agtggaatgt gttctgtctg tcagggaatc
     2881 ggtaagacga ttgcattttt aataggattg ctttattttt cggctattga taatgctgat
     2941 tttgtatttt ctgatccatt taccgcagaa gttttgtctg gaattgagga acaggcaaag
     3001 aatcatggtt attttgtatt ggtgcataat gttaattcag cagaagatgt taaaactatt
     3061 cagagaaatt ggcgttttga ggggttcatt gttgttggtg tacgtgtaaa agaatttttg
     3121 gaattaaata acgtttttga tggtcctgtt gtttatattg atacgcatct ttcaaaagag
     3181 acattaagag aacttgaaca acaaatgaat cgtttatttg taaatacaaa tgattaccaa
     3241 gctagtacat tggcagtgga acatttgatt cattatggtc atgagaaaat agcgtttctt
     3301 tcttttgact acattgtagg tgaaccaagt gttattcaag aacgacatca agcttatatc
     3361 gatacattaa agaaatatca tttaaccttt aaatcgcatt ggatatataa caactctgaa
     3421 tttgagcgaa tttatcaaga attagctgaa tttactgcaa ttgttgttac tggagattat
     3481 ttagcggcta aattagtaaa atttttaaaa aataagaata aacgagtgat ggaacaatta
     3541 tcgattattg gatttgatga tattactttt gcagaattaa tggatcctgc gttgacaaca
     3601 atacgactaa atccaggtaa caagggggcg attgcttttc accaactgat tggactggcc
     3661 aaacaagaag aaataaaaaa tcaagtacta ctattagagg gagaattgat ttctcgagac
     3721 tctgttcaca aaatcaaata aaaaagctct gtgaaataag tatagttaac aataaaatat
     3781 ccgaacgata gagagaatga actgtgatct agcagttcct cgtcgcaaat tcactagaat
     3841 tagcattatt tactctaaat tgttcggata ttttttcttg tttttaatta tgattacagt
     3901 gcctattttt aacgaaattt gcttattttc aaaatgtatg attatatgtt agtaaacact
     3961 ttcaaatatg aaaagaggag ctattatggt aggaattagg gatgttgcaa aacgggcggg
     4021 tgtgtccatt gcaacagtat cgcgcgtgct aaatgaggat ccgacacttt cagtaaccga
     4081 tgaaaccaaa cgaaagattg cagaatcggt taatttttat cattatgtca aaaaaagtcc
     4141 cgttgcaaaa aatttaacaa atattgcatt aataacgaca gtttctgaag tagatgagtt
     4201 ggaagatccc tattttcgaa cgattcgcag agggattcaa gtcgaagcag aacaaagcaa
     4261 agtcaatttg aagaaaatta ttcgtttgtc agaaacaccg ttggagcaag acgatttgct
     4321 aaaatgtcaa ggtgtgttga ttattgggca ggtgctatct tctgtgattg aagaagtatc
     4381 agcaattaat tcgaatgtcg tagtgattga tgaccctagt attgaagctg aagtagatgc
     4441 agtttatact gatttaacaa aagcaacgaa tcagcattta gatcgattgt ataaaaaagg
     4501 acaccgaaac attgcgttta tcggaggagg acgtgttcgt ttagatagcc aaggaaataa
     4561 acacgtgtca gatgatgatt atcgaaaaat agcctatgag acgtggatga aacaaaaagg
     4621 attagaaaaa tatagtcaaa tttatttaga aggctggacc acacttgacg gaatgcaggc
     4681 agcagattta cttttgaaaa aacatgctaa agataagttg ccgactgcta tcatggtagg
     4741 aaatgatcct attgctgtgg gggtttatcg ttcacttcaa aaaaatggtc tacatattcc
     4801 agaagatatt tcgattgtga gttttgataa tattgaagtt gctgaatttt taacacctgc
     4861 attaaatacg gtgaatgtga atacagaaga aattggaaaa ctagcagttc gcatggttaa
     4921 tgaacgaata aaagaagtta gaaatgtttc gatcaaagtg attgtttcta ataatattat
     4981 tgttagagaa agtgaaaaaa atatcgatta aaaaagtaaa catccaaaaa agggtgttta
     5041 cttttttgtt tactaatatt tttagtaaaa aatctcgaca aaacattaag taaacagtta
     5101 tggtgttgtt aacaaaaaga aaacgcattc aaaacaggag gaattaagat gaaacatttc
     5161 aaaaaaatag ctttaacagg gatagctgtt ttaactggga cattactcgc agcatgtggt
     5221 gggggagata gtaattctaa agacgggcaa gtaactttaa gctatgcgag ttgagatact
     5281 gaccaagctc caggactaag aaaaatgtta gatgaatttg aaaaacaaaa tcctgatatc
     5341 aaagtagaaa tggagacaac gccttgggat caatattggg ttaaattaga agctgcaaca
     5401 acgggaggta atatgccaga tgtagtaacc atgcactcta gcgaatccta taaatatatg
     5461 tcacagaatg ctttgatggg acttgatgat gttatcaaag aaaatgatat tgacatggga
     5521 aactttactg aaggaattgc tgatttctat acgcttgaag acgaactata cgcaatccct
     5581 aaagatgctt ctgtagtggg gctatggtac aacaaagaat tatttgataa tgcaggtgtc
     5641 gattatccag atgaaacatg gacatgggaa acactcagac aagctgcaat tgatttaaca
     5701 gatgaatcaa aaggcatcta tggttttgca tcacatattg gaacagagac tggctactgg
     5761 ccatttattt atcaaaatgg gggagaagta tttgccgatg acaatacaaa atcaggtttg
     5821 aataccccag aagcacaaga agctttagct ttttatcgag atattgcact tgttgatcat
     5881 gcttcaccaa caattacaga attacaagat cctgataagt taagtcgttt tcaatcaggt
     5941 agattagcaa tgatgattga aggaaactgg tttgcaccgg gatttttgaa aaatgactat
     6001 ctagttgaaa atgttgcttc tgccgtgtta cctaagggaa aagttaatgc aacaattaca
     6061 aatggacttg gttgggctgc cagtgcgagt acaaaatacc cagaagaagt gaaaaaacta
     6121 atggcatttt tagctagtga ggacgccaat aaaatccaag cggaaacggg ggcatcaatc
     6181 ccagcactaa aaggctttga agacgagtgg gctgcttctt cagatacaat tgatttgagt
     6241 gcttttggaa aaatgttgga gtatggagtc cctcgtccat acaataaaga tggattacga
     6301 gctgaacaag cagaagttca aattattaac cgtattctgt ctggtgaagt aactgttgaa
     6361 gatggaaccg ctcaaattga aaaagaagtc aatgcgatat tgagcggaaa ttaaattagt
     6421 taattataca atatacaggc gaaaggattc tatttcgtct gtatattaat ggatgaatac
     6481 caaaaagggg gatattatga aaaatcgaat ttttggaaaa tatcgtaaag atcaagtgat
     6541 atggggctat atttttattt ttccaacggc attaggatta attttattaa atatatggcc
     6601 agcaattcaa acagcaattt taagttttca aaaaacggta gggttcggtg atactgaatg
     6661 ggtcggatta agaaattacc aaaaaatgtt tgcagacggt gaagtttttc agtctttatt
     6721 aaacacatta atttatgcag tggtttcgat acctagtatt gttgtattat ccttgttggt
     6781 ggcagtttta atgaatagaa aagttaaagg attgtcgatt tatcggacaa tttattttct
     6841 accagtcgtt gctgcaccat ccgcagtcgc aatgatttgg cgctggatgt tcaataatga
     6901 ttatggactg attaataaca tgttggctaa aataggtctc gatggaccag catggttaac
     6961 ggatccatcg attgcgattt attctattat cattgtagga gtttggagtg ctattggtta
     7021 taacatggtg ttattactag ccggcttata agaaattcct aaagattatt atgaagcagc
     7081 ggaaattgat ggtgctagtc caatccagca gtttttcaac attacgttac cattagttac
     7141 accaacatta ttttttgtag ttgtaacaac agttatcaat gctttccaag tatttgatgt
     7201 tatttttatg atgattactc ctaatagtac cgccatgttt aaatcacaat cattagccta
     7261 tttattctat aaacattcat ttattttaaa tgacaaggga tatggttcgg caatcgttat
     7321 gttcctttta gtgattatat taattattac tgcaattcaa atgaagttgc agaaaaagtg
     7381 ggtaaactat taaggaggct atcaaattat gaaaaataaa tttttttcaa atgcgccaat
     7441 ccatatcatg cttattttag gtggtattgc aatggcatta ccttttattt ggatgatttt
     7501 aacttcaggg aaaacgttgg ttgaatctac ccaaattcca cctacaattt tccctgagaa
     7561 atttcagcta agtaactatc aacaagtact tgatttatta ccattcatgg ctttttatat
     7621 caatacattt ttaatggttg ttggacgagt gattggctcg gttttatttt cagcaatggc
     7681 tgcatatgct tgtgcgcgtc taagttttcc agggaaaaat atccttttca gcttagtact
     7741 tattcaaatg atgattcccg gttccatttt tattactcca cagtatttat tagcacaaaa
     7801 attgggtatt ttaaatacaa ttccagcatt aatattacca ggggtagtta gtgcgtttgg
     7861 tacgttttta ttaagacaat ttttcttagg tttacctgat gaattggagg aagcggccta
     7921 tttagaaggt gccaatattt ggacgatctt tttcaaaatt atgttgccgc ttgctaaatc
     7981 aggattggtt gcacttggaa tattcacagc gttgtttgca tttaaagatt taatgtggcc
     8041 attgattgtc aatacttcaa ttgataagat gcctttagct ccgggattag caaacttaca
     8101 aggacaatat gccacgaact tcccacaatt aatggcaggt tcgttattag caatttggcc
     8161 aatagttgtg atattcatcg ttttccaaag aaaattcatt gaaggaattg ctacaacagg
     8221 tagtaaatta taaaactatt aattagtgat tggagagtaa gtacatgaca aaaattacat
     8281 ttattggtgc aggaagtact atttttgcaa aaaacgtatt aggcgatgcg atgttaactc
     8341 caagtctaca agatgcacat attgcattgt acgatattga tgaaaaacga ctaaaagaat
     8401 caaatttaat gcttaaaaat attaataaaa attcgaatga aaatcgtgct gatattgttt
     8461 cttattatga tcgcaaagaa gcgttaagag aggctgattt tgttatcaat gctattcaag
     8521 tgggtggtta taaaccttca acagtgattg attttgatat tcctaaaaaa tatgggttgc
     8581 gacaaactat tggtgacacc atggggattg gtgggatttt tagaacccta cggacactac
     8641 cagtaatgtt agactttgct aaagaaatgg aagaagtttg tccaaatgct tggttattga
     8701 actatacaaa tccgatggcg atgttaacca tgggtgtttt aaaagcgacg agtattaaga
     8761 ctgttggttt atgtcatagc gttcaagtat gcgtaccaga attgtttgag catttaggaa
     8821 tagccgatca atatgactta aatgactttc aatggaaaat tgcaggcatt aatcatatgg
     8881 cattcttgtt ggaaattaca aaaaatgggg aagatttcta tccaacaatt cgtcagttag
     8941 ctgcagaaaa accaaatcca catccagatt ctgtacgttt tgaattgttg aaacgttttg
     9001 gttactatat tacagaatca agtgaacaca atgcagaata ccatccatac tttattaaat
     9061 caaaataccc agaattgatt gacgaattag ggattccgtt ggatgaatac ttacgtcgtt
     9121 gtgaacaaca aattaatgat tgggaaagca tgcgagagga cattgtaaat aatggggagt
     9181 taacacatac gagaagccgt gaatatgctt catatatcat ggattctatt acgacaggaa
     9241 ctccgtcagt gattgcagga aatgtcttaa ataaaggctt aatcacaaac ttaccgacag
     9301 atgtttgtgt ggaagtacct tgtttagtcg ataaaaatgg cgtgcaacca acctatgtcg
     9361 gtgaattgcc aacacaatta gctgctttaa atcggacgaa tatcaatgtt caagaactga
     9421 cagtagaagc cgctttaaca ctagataaat caaaaattta tcaagcagcg tatttagatc
     9481 cacatctatc atctgaattg tctttaacag atatcaaaaa tttagtggat gatttaatcg
     9541 aagcacatgg ggactattta cctgaatata attaaaaaga agctgaaaga gtaaggaatt
     9601 ttcaattcct tactcttttc ttagttaaat ttagtaaaaa tattgacaaa acacttagta
     9661 aacagttaca ttagtgttaa caaaaagaaa acgcattcaa attgtataag gaggagcata
     9721 aaatgaagtg gtctaaaaaa attattttag cttgtatagc actaggagca acgatctctt
     9781 tgactgcttg tggaaaaaat caggaagatg caagcggtaa ggtagaaata gagtttttta
     9841 atcaaaaaaa agaaatgact caaacgattc aggagattgc taaagatttt gaagcaaaaa
     9901 accctgatat tcatgtaaaa gttgtggatg taccgaatgc tggtgaagta attaaaacac
     9961 gtatgcttgc aggcgatgta ccagatgtca ttaatcttta tcctcaaagt atagaattga
    10021 aagaatgggc gaaagctggt tatttagagg acttaaccga agaaccgtat ttagagaata
    10081 tcaaaaatgg atatgcacaa cgttttgcta ttgaggatcg tgtatatagc ataccattaa
    10141 ccgctaatgt ctatggtttt tactataaca aaactgcttt tgaggaaatg gggattcaag
    10201 caccagaaac ttgggcagaa tttgaaaaaa tagtagctga gattaaagat caaaacaaag
    10261 taccgtttgc gattgcagga gcagaaggct ggactttaaa tggttatcac caactagcta
    10321 tagcgactgc tgctggtggt gaagaggaag cgaacaatgt ttggcgtttc tcagaagtta
    10381 atggaattaa tgctgaaagc aaagaaatgc aaaaagattt taatcgatta gatttgttaa
    10441 gagaaccaaa agctttgcaa aacaactggc aaggtgccgg ttataacgat acagtagtta
    10501 cttttacaaa aggtgatgcg ttgattatgc cgaatggttc atgggcaatg ccgatgattc
    10561 attcacaaaa tccagatttt gaagtaggaa cttttccttt tccagcagat gaagcaggtc
    10621 aaagtctaac gattggcgca ggagacctag ccttatcagt ttctgcaact tctcaacata
    10681 aagaagaagc caaacgtttt gtagaatata tgacaacacc tgaagcgatg cagaaatatt
    10741 atgatgtaga tggtgcaccg tgtgctgttg agggcgttat cgagaatact caggattcac
    10801 cattaagtgg tttaactgaa cttgctttta ctgatcgtca tttagtttgg ttagccaaag
    10861 attggaatag tgaaaatgat ttttatacct taacaacaaa ttatttacac aatggcaatc
    10921 aaaaagccat gattaacgca ttaaatgctt tctttaatcc aatgaaagct gacggaaggt
    10981 aggaaaataa aatgaaaata aaagaaattt taaatcgtta ctgggcaatc atttttgtag
    11041 caattccaat tattttgcag atgatttttt tctactttcc aatggttcaa ggtgcttttt
    11101 acagtttaac taattggact ggtctgactt ataattttga ttttgtgggt ttgaacaatt
    11161 ataagattct tttttctgat ccgaagttta tcaaatcgat tggcttcact ttaattgtga
    11221 cgctttgcat gattgctgga caaattggtt ttggtatttt gattgcgcgg gcgttgaacg
    11281 caaatattaa agggaaaaca ttctttagag catggttctt ttttccagcg gtcttatcgg
    11341 gcgtggttgt ttctttagta tttaaacaaa tttttaacta tggtttacca aaaattggta
    11401 gtatgttagg cattgaattt ttacaaacca gtcttttagg aacaacatgg ggagcgattt
    11461 ttgcagtaat ttttgtttta ctttggcaag gaattgccat gccagtcatc attttcttag
    11521 cgggttttca aagtattcca caagaaatta ttgaagctgc tagaatggat ggggcgaatg
    11581 ataagcaaat tttttggaat attgaaatca cctacttatt accaagtatt tcaatggtat
    11641 ttattatggc attaaaaggt ggcttaacag catttgatca aatttttgct ttaactggtg
    11701 gtggaccaag cgatgccaca acatctttgg ggttacttgt ttataattat gcttttaaaa
    11761 acaatcagtt tggttatgcg aatgctattg ctttagtctt atttatctta attgggattg
    11821 tttcactggc acaattaaaa ttatcgaaaa aatttgaaat ctaggaggtt tcccaatgaa
    11881 aacatcaaaa gaaaaaaata tttctaaata cttgctttta ttattagggg gcttcatgat
    11941 tttagttccc ttagtttcaa cgctattcgg ttcatttaaa acaacacccg atattatgaa
    12001 taatttcttt cgttttccta atccagcgac tttggaaaat ttccgaagat tgttagcgga
    12061 tggaattggt cattactttt ggaattcggc aatcatcaca atcattgcag tagttgctgt
    12121 gacagcaatt attcctatgg ctgcttattc actagcaaga aaaatgactc gtgggaaagc
    12181 atacggattg atgtatttat ttttaatttt aggaattttt gttcctttcc aagtaattat
    12241 gattccgatt accgttatga tgagtaaaat tggtttagca aatatgtggg gattaatctt
    12301 actctatctc acttatgccg taccacagac actattttta tatgttggtt ttattaaact
    12361 aaatgtgcct atcagtctgg atgaggctgc tgaaattgat ggggctagtc actttacaac
    12421 ttatttcaaa attgttttcc caatgttaaa accaatgcat gcgacgacta ttattcttaa
    12481 tgctctatgg ttttggaatg atttcatgct accattatta attttaaatc gtgattctaa
    12541 tatgtggaca ctaccattgt ttcaatataa ttatacggga caatatttta acgactatgg
    12601 tccaagtttt gcttcctata cagtaggaat tgtcacgatt acactagttt atcttgcttt
    12661 ccaaagaagt attatagctg gaatgagcaa tggagcagtg aaataaccaa taaattattc
    12721 tcattataaa ggagttcata aacttatgat tatatttgac aatcacacac aaacatttca
    12781 tttacaaaat aaaaaaatta gctatctatt aggaatcgaa gaaaataatt atttaacgca
    12841 tctttatttt ggaaaaaaga ttacgcacta ttctggtggg tatcgctacc cccgaacaga
    12901 tcgttccttc tcacccaaca atgcaaatgc gcaagatcgt ttattatctt tagacacatt
    12961 accattagaa tttccaggat atggacatgg tgattttaga gaaccggcgc ataatattaa
    13021 attaggaaac ggtagtcgtg taaatgattt tcgttatgat agctacgaaa ttcaaaaagg
    13081 aaagcccagc ttagcgggat tgccagcctt atatacggaa gatgatgatg aagctgaaac
    13141 gctgattatt caattgaaag acgcgattag tggattgact ttacagttgt tctacacaat
    13201 ttatcaaaat cacagtgtaa ttattcgttc tacgcaatta gtaaataata gtgatgaaac
    13261 agtggagatt aaccgcttag ctagtcaatc aattgatttt ccaaatcgtc cactcgagtt
    13321 aattcattta aatggtacct gggcaagaga acgtcaaatg actagagaaa aaattcatac
    13381 agggataaaa gtattagata gtaaacgcgg atcaagcagc catcaacaaa atccgtttgt
    13441 tgctttagta gatgccaaaa cagatgaatt tcaaggagag gcatatggat ttaatttagt
    13501 ttatagcggt aatcacgaaa cggtgattca aaaagatcca tttgaccaaa cacgggtcat
    13561 tatgggaatt aattcattta attttggttg gcaactaaaa actggcgact catttcaaac
    13621 gccagaagtc gtgatggttt atagtaatga aggtctgaat acgttatcac agacgtatca
    13681 tgaattgtat aatcaccgtt taattcgtgg aaactatcgt ttaaaagaac gtcccacgtt
    13741 aatcaacaac tgggaagcaa cgtattttga ttttaacgaa gaaaaaatca tgaatattgt
    13801 cgatgatgcg aagaacttgg gcattgaaat gtttgtttta gatgatggtt ggtttggcgg
    13861 tcgtgaaaat gacttcactt cattaggtga ttgggttgaa acagaaggca aacttgcttg
    13921 tggtttggaa aatttagcca agcaagtgca tgataacggg atgaaatttg ggatttggtt
    13981 tgaaccggaa atgatttctg aagatagtga actatttcga gcacatccag attgggcatt
    14041 gggtattcca ggtcgtgacc gctcaattag tcgaaatcaa tatgttcttg atttttcaag
    14101 acaagatgtt cgtgataata tctatcaaca aatgacggcg atttttgatc gtgtaccgat
    14161 tgattatgtg aagtgggata tgaatcgaaa tatgacagaa gtctattctg tcttgttgga
    14221 ctcaacgatg caaggtgaag tggctcatcg ttatatgtta gggttatatg aatttttaga
    14281 aaagttaaca acgaattatc caactatttt atttgaaagt tgttcgggtg gaggcggtcg
    14341 ttttgatgca ggtttcttgt attacatgcc acaaacttgg acgagcgaca ataccgatgc
    14401 cgtggctcgt ctgaaaattc aatatggaac cagcttagtg tatccaattt caagtatggg
    14461 tgcgcatgtt tcagcagtcc caaatcatca aacaggaaga gaaacgagtt taactattcg
    14521 tggcaatgct gcgatggctg gtgtttttgg ttatgagcta gatctaacgc aattaacttc
    14581 tgaagaaaaa gcagaaatta aagaacaagt tgccttttat aaaaagcatc gccaactttt
    14641 gcaatatgga actttttatc gcttggaaag tccgtttgaa ggcaatgata ctgcatggat
    14701 gtttgtctca aaagaccaaa cagaagccat ggtcttttat ttccgagtat tagcagaagc
    14761 tgcgcatcca ttagtcacat tgaaattagc aggattagca gaagaagcaa cttatcaaat
    14821 ggaagatgta acatttgctg gcgatgaatt gatgaatctc ggtttttata ttgactcaga
    14881 actacatggt gattatgcga cacaacgctt ttatttgaaa cagatatagt agcttgaatt
    14941 tacaatataa gtgcaacacc aaaagagact cattccaaac gaagaaaata tgttttattt
    15001 acacgcattt actaaaaagc agcctgattt gaattgggaa aatgaagaag tacgtgaaga
    15061 aatctatgca atgattaact attggttaga taaaggacta ggcggattta gaatcgatgc
    15121 gatattaaac atcaaaaaaa aactagagta tggcatcttt gaatctgatg gagaagatgg
    15181 gttagccttc attgggccct ggattcttaa tcagtcgggc attgaagtgt ggttaaaaga
    15241 aatgagagag cggacattta aaccacataa tagtatgacg gtagcagaag ccgatgttcc
    15301 taatgaacgt ttagatgaat atattggtga agatggctat tattcgatgg tttttgattt
    15361 tagttatacg gatattgatg tacctgaaac tggtgaatgg tttaaagata gccaatggac
    15421 atggacggat atgcgtacca atatttttac gaatcaatta gttacgcaag ataaaggttg
    15481 gggcgcctta tatttagaaa accacgatca accacgttca attaataaat atattccaga
    15541 agaatggatc aatgattata gtaaaaaaat gttggcgacg ctttttatgt tactaagagg
    15601 tacccctttt atttatcaag gacaagaact aggaatgacc aacatagaaa tggcatcatt
    15661 agaagatttt gatgatgtag ctacgcattc tcagtataaa cgggcattag aatatggatt
    15721 ggcaccagag caagcattga aagctgtcaa taaacgaagt cgggataatt ctcgcacacc
    15781 tatgcaatgg aatacgcaaa aaaatgccag attctcaact tctgataatg tatggttaaa
    15841 agtaaatcct aattatccag aactcaatgc agaagctcag ttaaccaatc aacagtcggt
    15901 attcaattat tatcatcaat taattaaatt aagaaaaaat agtgtataca aagacgtgct
    15961 aatctacgga caatttctac cagtgaaaga taaaaatgaa catattttgc tttatgaacg
    16021 ccaattagaa gataaaatcg tattagttat cttaaacgtt acagcaacag aacaagaata
    16081 ttatgtagat gaacagtacc aacaagtatt aatcaataat tatgaagatg ttttattaga
    16141 tcaaagtcga aaattgcgtt tacgaccgtt tgaaagtatt gtattagcta attatggagg
    16201 acaattatga aaattaaaaa cgaagcaatg ctcatcacct atgcagatag tttaggaaaa
    16261 aacatgggcg aattaaaaca agtcttagac aaacatttac aaggtgttat tggtggtgta
    16321 catctgttgc ctttttttcc atcgacaggt gatcgaggct ttgcgccgag tgattatacg
    16381 acagttgatc cagctcttgg aacatgggaa gatgtggaag cattaggtga agattattat
    16441 ttgatgtttg attttatgat taatcacatt tctcgtgaat caaaattttt ccaagatttc
    16501 aaaaaaaacc atgagcagtc accatacaaa gaaatgttta tccgtattca tgaatttttc
    16561 ccagaaaatc gaccaacaca agaagatatt gatttaatct acaagcgtaa agacaaagcg
    16621 ccgtttcaaa cggtagaatt tgcggatggt acgacagaag aggtttggaa tacctttggg
    16681 gaagaacaaa ttgatttaga tgtgaccaaa gaagttgtga aagaatttat tcgagaaacc
    16741 atcaaagata tggcgaacca tggctgttct ttaattcgat tagatgcgtt tgcttatgcg
    16801 attaaaaaat tagataccaa tgatttcttc gtggaaccag atatttggga acttttagac
    16861 gaagtgcgtc aagaagcagc gaaatatgac gtcgaattat taccagaaat ccatgagcat
    16921 tattccattc aaatgaaaat tgccaatcat gattattata tctatgactt cgccttgcca
    16981 atggtcactc tgtattcact ttatagtgga aaatcagaac gtttagccaa ttggttgaaa
    17041 atgagtccga tgaagcagtt tacaacattg gatacgcatg atggaattgg tgttgtggac
    17101 gctcgtgatt tattaacaga tgaagaattg gattacacct ctgaggaatt gtataaagtc
    17161 ggggcgaatg tgaagaaggt ctattcaagt gccaattaca ataatttaga tatttatcaa
    17221 attaacagca cctattatag tgcattaggc gataatgacc gtagctatct gttagcacgt
    17281 gcgattcaag tatttgcgcc agggattcca caaatttatt acgttgggtt attagcagga
    17341 aaaaatgata ttgaactgtt ggaagcaacg aaagaagggc gtaatattaa tcgtcattac
    17401 tatgatttag ccgaaattga agaagaagtg caacggccag tgattcaaca attatttgca
    17461 cttttaaaat ttagaaatga atctgcggcg tttgatttgg atggaacgat tgaagtagca
    17521 acgccatcag aatcagaaat agtgattaca cgtaaaaata aaaacggtag tgatagtgca
    17581 acattaacgg ctaacttgga tacgaaagaa tttatcattg agtctaaaga tataaaaatt
    17641 ttcactagtg ttgcattaaa attttaacct attccataga acaaaaaatc ctttatacta
    17701 gcacttggat gacttatcca agaccaataa aaaggactca tacatggata agtataaaac
    17761 aaatacagca tttaataaat ggttttctgc cataaaatta ggaaaacttt ctgtcaactc
    17821 ccaaaaatgt atttttgatt acaattcgta tcgtaaaaaa ctgtcttttg aagctgtgct
    17881 caagctcttt ctctacgcag ttaatgaaga aaaagaaagt ttgcgtgatt tgagtacagc
    17941 tcttatcgac cgatcgctcc aactagaaac cgatgtgtca tcgattagtc atacccaatt
    18001 gtctcgagca tttaatgcac ttgaacctaa agtgttagaa gaaatttttc aacaactgtt
    18061 agaaaaagtt caacatcaaa tcaaaccaac caagcgaaat catctgtatc tgattgattc
    18121 cagtacgttt tcgcttagtt taaaacgtca taagtgggcg actttccgtc aaacaaaatc
    18181 aggtgtcaag ttacacttaa actattgtta tatggacgat actacgatgt atccaactga
    18241 ttttacctta acaaatgccc atgaacatga tctcaatcaa ctaccagtat tggtgaatca
    18301 accagaggca acttacgtgt ttgatcgtgg gtattaggac tttgaaaaga tggatcagat
    18361 gcattgggat ggctatttct tcgtcactcg gattaaaaaa aacaccaaag ttcacgtgat
    18421 agatactctg gaaacatcgc cagaaacaga aattctacga gacgagctgg ttcgtttagg
    18481 atctaaaacc tatctaaccg ccaatttccg actagtcact gttcaagata agaacggacg
    18541 tgtgtttcaa tttatcacca atcgaatgga cgtctcttca aaagaaatct ctgatatgta
    18601 tcacgctcgt tggcaaatcg aattgttttt caagcacatc aaacaacaca tgaccattaa
    18661 aaccttcttt tctcaaagtg aaaaaggcgt gcagaatcaa ttgattttaa cgatgatttc
    18721 cgctctcctc acgtttctca tcaaattgga gacgaaaacg gaaaaatcag tgttccaaat
    18781 caaacgattc tttcgttatt tactgtttca gccctttgaa tgttgtctag aaaaattaat
    18841 tcccacatga gaaaaaagaa atttggctcg ttacttttgg ttttcagttg aataacagat
    18901 aaaaaatttt ctggaaaaag tcatcctctg cgtcagcaca gttttttttg acttattttc
    18961 agaggaaata gttttcagaa tattctaaaa tgttttatgc aacactagtg atatttttta
    19021 gaattagatt tctaatgtgc attttttgag tattaaggag caagtataca aagatttata
    19081 aagctgatta aatttgaata aaaatatgca ggaacgctac tatttttagt agtgttcttt
    19141 tttatatttg aataaaaaat gtggtggtta aatggtggtt ataacaaaaa atgaattaat
    19201 gaaattaggt tatgggaagt atcagtctga gagaatcata cgacaggcaa aagcaattat
    19261 ggcttcaaaa agctatacct attacgaaaa tagaagatta ggactagtac ctgttgaatc
    19321 tgtagaagaa gttttaggta tgaagataga ggtagaggag gattttcaga atcatggtta
    19381 attttaaaaa agataaaaat gggaaatatc attttactgc tagtttggga ttcgacaaac
    19441 gaacagggaa aagagtacaa aaaaacgtag aggggttaaa acagttaaag aagaaaaaaa
    19501 gcgtataccg aacttgtttc taattatgga aaagaagcag aaacttctca aagtactatg
    19561 ttgtttgagg agttttttta taaattattc ctaccttatt atcaagggaa agtagaagaa
    19621 agaacatata aaaatcggtt atctacaatc aaaaaaactt ttcttatttc tttaaaatgg
    19681 agttaagaga aatacgtcca attgacattc aaaagtggca aaataattta ttgagcaaat
    19741 ataaaaattc ttatgtaaga aacatgtatg ggatttttca gatgtcatta gatcgagcag
    19801 ttgttttggg actacttgaa aaaaatcctg caaaagttat tggaaatgtg aagaaaagga
    19861 aaactacagt tgatttttgg acaaaagaag aatttacaaa agttatttca actttttata
    19921 ttgatgattt gtaccaacat tttggtttta tttgtatgtg gttacttttt atgaccgtta
    19981 tgcgtttggg agaagccact gcattaactt ggaaagatat tgattttgaa aataaaaact
    20041 tgcgtgtgaa taaaacactc tattataaga gagccaaaac ctatgagttt gtagaacccg
    20101 aaacggcttc tagtattcgt acaatcgaac tagacgacaa tacaattacc tttttaaaag
    20161 cttggaagga gcgtcaagtt tcaataggag gcatgtcgtt tgtactttct tataatggaa
    20221 cacctactca gaaatataca atccgtcata ttatgagaag acatgcaacg atcgttggtg
    20281 ttcatataat ccgcatacac ggattaaggc atagtcatgc ttctttgctt atatcacaat
    20341 aaaaagatgc acttcgaatt aaagaaaggt taaaacatgc tgatgtacaa acgactttag
    20401 gaacttatgg tcatttgtat ctaaattcat tgagagagat aacaaatgat cccacagatt
    20461 atatccaaac taatttttct aaaatcaaac cgacacctat ttacgataat aaaaaataaa
    20521 tgataaacaa aagtaggatc acaccaaaag aaacacacta gaaaaactat aatacaggct
    20581 tttctagcgt gttgtttctt atttccgcct atttatacat tatactcata aagcatttaa
    20641 tgcatcgatc gtagccgcat tgtctatatg ggtatataaa gttgttgtat tggtacttga
    20701 atgtcccaat tgttgggcag ttaataccaa tgaatttgtc tgttggtaga gtctagtagc
    20761 taatgtatga cgcagtttgt gagggctgac ttgaattcta aagccttttg aatattttgc
    20821 taccatccgt tcgattgcaa taccggatat acgcttggct tctccttggt aaattgctag
    20881 aaataaagct ttttcgtttt ctgttacttt atatcgttct ttccgaatag ctagataatt
    20941 agctaaatat tccattgcga atgctgctat attgacagca tctcttttat ttccttttct
    21001 agttactacc acattattct tatctaaaga taggtcatct aaattaatat ttataacctc
    21061 ggagagtctc aatccagatc ctaaaatcag tgcattgatc gccaggtctc tttctacatc
    21121 tcgttgataa tagatcaaag ctcgtttact caaagtattt ttatattcat tggcaatata
    21181 atctagataa ttttgtgtat ccttatctag aaataatttt ttctcaatgg cattggcacg
    21241 tgcagctaac gtagttttat ctgtgatggt tgctacttga tccataggat tattgtctaa
    21301 gtatggtttt cctagaggat tattggaact tttcgaaaga tatttaaata gaacctttaa
    21361 tgccgtgata tttctattga tagtagaagt agatagacct aagttattat tatctttgag
    21421 cttagttttt gttgtctctt tttttacatt ttcttttgtg cgacgtttca aatgagattt
    21481 gtacaagttt aggtcatagg cagttaactg tcttatcgag gtgatagaag tatcaggaac
    21541 gatagactca ctcagaagcc attcataaaa gagcttataa ttttttagat attctaaagt
    21601 ggttcgaggt gattgatctt tatcctcacg atcttgaaca aaatctttta tataatcggg
    21661 catatgaagt atgattgtat ctattttttg atatatttct gtatttttca tgtcattcat
    21721 cctttcaata agtaggggag aagagtatct ctataattgg actataccaa ttatagaatg
    21781 ttttgtcaat tggtatgctc taaaatttat taatttgttt caaaaaaagc atataaacag
    21841 gtata
//
LOCUS       DAZBTF010000048        21729 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000048 DAZBTF010000000
VERSION     DAZBTF010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.048"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(177..1076)
                     /locus_tag="ACHGNS_001839"
     CDS             complement(177..1076)
                     /locus_tag="ACHGNS_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFN6937774.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1086..1442)
                     /locus_tag="ACHGNS_001840"
     CDS             complement(1086..1442)
                     /locus_tag="ACHGNS_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFN6937775.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1797..2093
                     /locus_tag="ACHGNS_001841"
     CDS             1797..2093
                     /locus_tag="ACHGNS_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFN6937776.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(2737..3690)
                     /locus_tag="ACHGNS_001842"
     CDS             complement(2737..3690)
                     /locus_tag="ACHGNS_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN6937777.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(3713..4468)
                     /locus_tag="ACHGNS_001843"
     CDS             complement(3713..4468)
                     /locus_tag="ACHGNS_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6937778.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(4465..5424)
                     /locus_tag="ACHGNS_001844"
     CDS             complement(4465..5424)
                     /locus_tag="ACHGNS_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFN6937779.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(5417..6367)
                     /locus_tag="ACHGNS_001845"
     CDS             complement(5417..6367)
                     /locus_tag="ACHGNS_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFN6937780.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            6517..6969
                     /locus_tag="ACHGNS_001846"
     CDS             6517..6969
                     /locus_tag="ACHGNS_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFN6937781.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(7058..9325)
                     /gene="gshAB"
                     /locus_tag="ACHGNS_001847"
     CDS             complement(7058..9325)
                     /gene="gshAB"
                     /locus_tag="ACHGNS_001847"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFN6937782.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(9512..10510)
                     /gene="ftsY"
                     /locus_tag="ACHGNS_001848"
     CDS             complement(9512..10510)
                     /gene="ftsY"
                     /locus_tag="ACHGNS_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFN6937783.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(10521..11330)
                     /locus_tag="ACHGNS_001849"
     CDS             complement(10521..11330)
                     /locus_tag="ACHGNS_001849"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN6937784.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(11339..14920)
                     /gene="smc"
                     /locus_tag="ACHGNS_001850"
     CDS             complement(11339..14920)
                     /gene="smc"
                     /locus_tag="ACHGNS_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFN6937785.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(14940..15626)
                     /gene="rnc"
                     /locus_tag="ACHGNS_001851"
     CDS             complement(14940..15626)
                     /gene="rnc"
                     /locus_tag="ACHGNS_001851"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFN6937786.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(15974..17755)
                     /locus_tag="ACHGNS_001852"
     CDS             complement(15974..17755)
                     /locus_tag="ACHGNS_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN6937787.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(17781..18695)
                     /locus_tag="ACHGNS_001853"
     CDS             complement(17781..18695)
                     /locus_tag="ACHGNS_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6937788.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(18722..19684)
                     /gene="opp4B"
                     /locus_tag="ACHGNS_001854"
     CDS             complement(18722..19684)
                     /gene="opp4B"
                     /locus_tag="ACHGNS_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFN6937789.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(19684..20628)
                     /locus_tag="ACHGNS_001855"
     CDS             complement(19684..20628)
                     /locus_tag="ACHGNS_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6937790.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(20629..21633)
                     /locus_tag="ACHGNS_001856"
     CDS             complement(20629..21633)
                     /locus_tag="ACHGNS_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6937791.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         6073 a         4641 c         3848 g         7167 t
ORIGIN      
        1 aaaaagaata cacgcttgca tctagtactc tgcgagttac aaatcatccg atcaaataaa
       61 cattcgctga ttattttccg caaaaaaagc agcatatcca cgataaatgg atctactgct
      121 cttacttatc cttatgtttt acaaggtatt ttcttttaca cattactaat gctgtctcaa
      181 tacggaacga ttgtcatcgc ttgttcaaat tcctcttgca aatgttgtgt ttgataggcc
      241 tgaaaagctg ggtgatgggc acgcaagagg acacgtaatt tcataggatc atagctgtct
      301 agcaactgtt ctttcaagac aaaatctcgg gtcagctgcc attcataata ataaccaaaa
      361 ggtctgcagc tgtaaatcgt gtgaccgatg gatttatctt caaagcgacg atgcgtatgg
      421 ccaaaaacta cttgtttgat atttgaataa cggtcaagta attctccgaa tgattctgat
      481 cctaaaaatg catttaattt gttccaacgc tcataaggag cagattgata catgataaat
      541 gcccgtttag gaacgaaatg agtggataaa atcacctcaa gtttttttgc ttccagctgg
      601 tctagcaaat tttttgcagc tgcaagcatt tctgcattga cctcgagatc tgttttccca
      661 cgctggatga accgatcata ccaatacaaa cgcttcattc gtacgattgc ctcgtaatct
      721 ttcaattcgg aaaactgata gtcgtaccag ccattcatcc ctaaaagaac ggtctgctca
      781 tttaaagcaa tataccgttc atttaaaaaa tgtgggtctg gaaaatggtc catcatctgt
      841 tcattgggca catctgccaa ctcgtgattg ccgaaatgaa atgtcgtaga taatccttta
      901 ttgacgaaaa attcgacaac agctaggcaa cggtctactt tattagccgt atctccggct
      961 agatgaagcc gagtgatctt gtgcttttgc aaaacttgcc aaagctgcat cagctctgat
     1021 tcgccgaatt tattgatgtc cacatgtagg tcgctgatga tcgccaattt tcccatttta
     1081 tccctctagt cttttcgaac aacttgcaag ttgccttttc tcaagccgct tggtcctttt
     1141 aattgtaaac tattataagt tggttctaaa gcaagtgtgg atggatcatc catgtaaagt
     1201 attgcactgt ctttgatttt gattggtcca actggtgtat cagccactac agaataaaga
     1261 cttagatctg tttgtgtatc ttttttgaca atgatcaatc gaaatgccgt atccaattgg
     1321 caactgactt ggctttctgc aaatggaccg tcaccgtttt cgatatctaa taaaagttga
     1381 tcgtctgctt ctaatttatt cttcaatact tcttgtgctt cttttgtcac ttctaatttc
     1441 atctgtatca cctcatgtca tagaataatc actttattca tcagatgcaa ctaatttgtc
     1501 tggttctgac tgttctcaat ttattggaaa gtaagtcatt cgtttattat gatttctctt
     1561 atcacaaaag agacaacttt cccttttctt cgttcagttt cttgaacgga aaaagaccca
     1621 aaaagcaacc tgttatttgt ttaaccttat tcctttctta aataacaaaa aatactataa
     1681 aaaataaaaa acctttaaca actattggaa aaagaattac tttttgttca cggattatta
     1741 acaactcttc ggtagagtaa acttatcaaa taaaagactt ctttggagga atcactatga
     1801 aggaatatat aaaaaacatc tattttatcg aagaaacaca aaatatcgag ggttcttata
     1861 ttgaagtaaa aacactcttt gtcaatgaag ataaaaccaa agcattggat atttataaaa
     1921 aattagcttc aaaaaaaacg aatagttttg gattgatatt aagcgagtat aaaatcaaag
     1981 cagaagagag ttatttctat caattactaa aacggtggag taagcttccc gccgattttt
     2041 atcgaaaaat gcagatcatc aactatcaac cgcttgctga gacacatgcc taaatagagg
     2101 ttgtgaaatc agtgctttga gctaataccg aaggtcagct gattgaacat tgtttagaag
     2161 agattgtgac aactgcgatt tgctttgagt aataagaacg ctggacagaa aatagcttct
     2221 catattttct agtctagtgg gcttattgcc gagaagcagc agatggaaca tcgtttagaa
     2281 gagattgtga caactgcgat ttgttttgag taataagaac gctggacaga aaatagcttc
     2341 tcatattttc tagtctagtg gacttattgc cgagaagcag cagatggaac atcgtttaaa
     2401 gaagagatcg gtacaaaata aaaatatcta aatttaagtt ttatctaagt ttcgctttgt
     2461 acctttgatt caaatcaaag gaggcgatct tgatgctgaa aaaatggatc acactatttt
     2521 ttgccactcc ccattctaga atcaacccgt atgatgtaga taatgaattc acgttgaaaa
     2581 aagatttctc aaataaataa aaaccatccg taaaatcatc aatacgattt tacggatggt
     2641 ttttgataac ttctttgagg ataggctctt gctatttaag aatgtaagaa acattattgt
     2701 tacatagtat gagagtttaa tcctctgtta tttatgctat ttaaatgctt gattcacatc
     2761 ttcgatcatc agtttcattg attctaaacc gcccccgctt aaataccaga catctggttc
     2821 aagggagatg atctgctgat ttttaccagc attcgtttgt gctaccagtt cattggcaga
     2881 aatatcatct ttgctgtcgt ctccaccaat agcttttgtt ctgtctacta caaataagat
     2941 atccggattt ttttccagca catattcata agaaacactt tgaccgtgag tggatgcttc
     3001 gatttgatca tctgcttgtt caaagccaaa taaatcatgg atgaaaccga atctagaacc
     3061 agatccgtag gctgataatt gcccttcatt cacaagtgtg actagtgctt ttttgtcagt
     3121 agcttctgct ttctttttcg tctgatcgat tgcactagat aattcctcta attgagtttt
     3181 agcttcttct tgcttaccaa agatctcccc taaagcagtg acattttgct ggaaagattc
     3241 ccatggattt ttcgcatcta aagaaaggaa aatggtcgga gcaatttgac ttagatcttt
     3301 ttgaaaatct ctttgtcggc cagaaatgat gattagatct ggctgaagct gattaatttt
     3361 ttctaagtct ggttctttta ttccacctgc tgattctact tttttatagg atgaaagata
     3421 ctcaggaagg ctgcttgtag ctgctccaac taccttatct ccaacaccaa gagcatccat
     3481 cgtatctaaa gaaccattgt cgaatacgac gactttttct ggattatttg gcacctcgat
     3541 ttggtctcca ttactatctg ttatcgtcaa tgtagtttca gtttgtgtgc tgcttttttt
     3601 tgtgtctgcc tgactggtgt tgttgctatt tccacatgct gctaaaccaa atgatgctag
     3661 aatcaagata cctactacta attttttcat ctcttctctc ctaatgttag ttttagaaat
     3721 aaagacagaa tctgcggcct tcaatctcac aaatccgtat gtccatttcg tataattcat
     3781 ccaatacatc tttttttatc atttcttggg tcgttccttg cgtaaataac tgtccatctt
     3841 tcattgcaac aatttcgtct gcataacttg cagcaaagtt gatatcatga agaacaataa
     3901 caattgtttt cccgatttct tgtactaaat tttttaaaat ctgcatgatt tgaaccgcaa
     3961 aattcatatc tagattgttc aaaggttcat ccagtaaaat ataatccgtg tcttgcgcta
     4021 gcaccattgc aatatacgca cgttggagtt gaccgcctga aagagtgtca atcgattgat
     4081 ccgctatttc ttctaaccca aggtgttcca gtgattcctg aacgatttcc tcgtcttttg
     4141 tcgtcaattt tcctttcgta taaggaaatc gtccaaattc tactaattct ttcacggtga
     4201 tttggacatt tatttcattc gattgtcgta ggatcgataa ttttttggct aattcattgg
     4261 atttccaagc ctttacttcg ttatggtcta aatagatttc acctgtatct tttggtatca
     4321 aacgactgat catcgacaac accgttgact ttccagcacc atttggaccg ataaaagcag
     4381 tcaacttctt ttcaggaatc gttaactgta catctttaac cactgatttt ccatggtaca
     4441 gtttattgac cttttgtaaa tcaatcatag gatactcttc ctttcgcgaa tcaattttgt
     4501 gacaaaataa attcctccga caaattcaac gacgacactc actgttgtat tccagccgaa
     4561 aatctgctct actgcgagct gtcctcctgt cagaaacagc atagctacca gaaagcttcc
     4621 gataaatagc tgaccatgac gataagtaga aaagacacga taactgatat ttgcgacaat
     4681 aaaccctaaa aaagtaactg gacccacaag agccgttgat gtccctgtca aaagactgac
     4741 acaacataaa atcaaaaatt caaaacgatc tgtgtctatc ccgaggttta ctgcttgttc
     4801 atttccgaga tggaaaacgt ctagcacatg tcgatatcga aataacaggt accagcagcc
     4861 gataatgatc aaagaagcag gcaaaagata ctgactctgg atattgccaa agcttgcaaa
     4921 taatctgcct tgaagcaaat catattcatt cggatccatg atgacttgta aaaaggtact
     4981 gacactacca aaaagcgtac ctaaaatcat ccctgacatc agtaataaaa aaagattcgc
     5041 tctcattttt ttcaaaagaa agaaaaataa caatagactc agcccgacca tcagcccaac
     5101 gttcaataag aaaaaaactg tacctgtctg ctgaagcatc gtcactcctc cgataaagaa
     5161 gaaaaggacc gtttggacaa ggacatataa cgactcaagt cccaatactg aaggagtcaa
     5221 aaactggtta tgcgtaattg tctgaaagct gattgtggca ctgctgacag ctagcgagac
     5281 aaggataaat gcaaaaattt tcttactcct caatgcacat gcaaaatgcc agtttccata
     5341 tgtatggatc gtcaagtaca ttcctgtaac aaataaaaca agaacaagca taccaataaa
     5401 aatagatggc ttttttttaa gcatatcgtc gtccccttat cagaagataa aggaagatag
     5461 ccccaccgat gattccaacg atcacactga ctggtatttc ataaggaaaa atcaacacgc
     5521 gagcgacaag atcgcaaaat actaagaaca aactacctgc taaagcagtt acgccaattg
     5581 ttttgtgcac ttgatctcca tatattctag agacaagatt cggaatgatc actccgataa
     5641 aaggaatgct tcctaccatg atcaacatga ctgcactagc caatgatact aagccaatgc
     5701 ctaaaaactg catcttctgg taatctattc ccagatttac tgcaaacgat tcgcccagac
     5761 caatgatcgt aaatcggtaa gcaaatagcc aaaccaagag caacaatgga acaacagcat
     5821 acaataactc ataatttcct cgtatcacag tcgcaaaatt cccttgaagc catgaagtca
     5881 tattttgtac caactggaat tgataagcaa aaaaagtagc aactgaacca atgatattcc
     5941 ctaacatgac tccaatcaac ggaagcatca attgattttt ctggaggaac aatcgcgtaa
     6001 gggataaaaa aagcaatgtt cctgcaaaag caaagcaaaa tgctgtcaag gatcggacaa
     6061 tcaaggatgc accaggtagg aaaatcatag ccactaaaat acctaaacgc gcactatcca
     6121 tcgtgcccgc agtcgttggt gaaacaaatt tattttgggt caaatgttgc atgatcaacc
     6181 cacaaatact actggtcgct cccacaagga tcaaacaaat cgttcgtggc aaacgagtca
     6241 ctagcaagat ttgtaagctt tgcccatcta actggagcag ttctgacaat tgtacattcg
     6301 atactccgat gaacaatgaa atactactta aaataactaa taatgctact agtcgtccgt
     6361 agcgcatcca atccaccttt ctaattgaga atgattttca ttcttatttt aagtgaatcg
     6421 ttgaacaaaa gcaaccgctt tttcatatgt tttaggtatt attgggaata tgctataatc
     6481 gtaagaaaga caaagagaag aaggagggta ttttttatgg cacaagatga tgtaaaaagc
     6541 catttggata agggacttta tggtacacct ttagtgaacc ctgaagaaca gcataaatat
     6601 ttagggacat tcagggaacg ctgctatctt agtatgactg ttgctgaaat gatccaacca
     6661 aaaaacaaag aaaacttttt aaaagaactc aaaaaacatc cagatgcttc catactcttg
     6721 aatggggctc tacccctttc tgtccaaaac caatatatcc agctagcaac gaaaaacaat
     6781 agccgtttca ctgtagtcaa cgattacgtc actgcaaatc ctgaggaatt cggattgctc
     6841 ttaacagcaa aagaagcagt aaatgaacct gtgattgata tcgaaaaaaa atacccagaa
     6901 gaaaactctt cgccagctga aaaagaaaaa ccaaaaaaga aaaaaagctt ctgggatcgt
     6961 ttattttaaa acgaaaaaac acataaattt ttacatgcaa aaaaagcttt tgatcagatt
     7021 gacatcaaat aggacaatct gtcaaaagct ttttctttta aaatacttca ggatataaca
     7081 atttcaatac attcatcgtc aatcttctgc cttttcctgc aaaaggataa acatgcatat
     7141 gcatggctgg attgaaattt gcttcgatga tcccatatgc ttttttatca gttgtaggat
     7201 cgatttcttt atctggtata atcaaatcga taccactaat cttagctcct agtgcttcta
     7261 ccgcagaaac ggcgattttt ttgtaagctt cagaaaattc atcagtcata tcgatcgaat
     7321 caccacctgt actgatattt gagttctccc ttagataaac aatttgattg gcttggggaa
     7381 ctgattcgat cgtcaaccct tgttctttca acatcaattg ttctaattca cctaattgga
     7441 tcaattctaa aggtgcacga tgattcgttc cacgcaaagg gtcactattt ttttcttcaa
     7501 caagttcttt cactgttcgg atactgtcac caataacatt agcagggact cgcaacatga
     7561 tggcttggac ttctccgtcg atcacaaaga aacgatattc cgttcccggc ataaattctt
     7621 ctactaaaac cgaactgtct tcacgaaaag caatggctaa acctgcttga taatcttcca
     7681 gactagctcc ttccttgaaa atcgtgatac ctaacccgta attggttgat ttaggtttga
     7741 tcacaaatgc ctgctcggca aatctcggat aagcttttac cgcttcttcc atggatgaaa
     7801 attctgcgcc tcctggtacc cggaacccag cttcttttaa gacttttttc gttactgttt
     7861 tgttttccat gataagtggg acgatatagc tgtccttact tgtcatgttt gcatttttca
     7921 catattctac atggtcttgg aattgcagac gcaaaaattg atccgactca tctaatactt
     7981 tgacttggac acctttttgg atggcatcga acataaatat ctgagtagaa agttccattt
     8041 cgcgaaaacc ggccaactga tagggacgtt catggctttc tttataatat tgatgtccca
     8101 atgccacagc aacttcttgc tgactcgata gctgtgcttc ttgatagagt ttcgctgcta
     8161 acgtttgttc aggatgatcc atttgagtca atgcttcctc tactaaataa agagaatcca
     8221 accaatccaa ttcaaccagc atttgcttca ttccttcaag aatttctcta ccttcttgat
     8281 gaaattctgt ttgatctgat ggttgttcta gtgctacttt gttatttcga gtcgtacctt
     8341 ctgctaccca ttggtcacag tcttcttttt cttctgtcca taacatgtac atcaggaata
     8401 attgtaagaa ccgaacttgc tctggattga tacccaatct tgcataagga ttcaaatcga
     8461 tattccgcaa ttcaatataa cgaacacctg ttgttgctaa atctgcgact ttctttccgc
     8521 ctcggaaacg aagcggtgta taaaattctt tctcctccga aagtttccct tcttcgatca
     8581 ttcgctcgat atctgctaaa tactgtttca tagaagcata ggaaactttc acatttggat
     8641 gattcgtata gcccagcgca ctgttgcgaa tactgcgaac aggttcttgc ggatgtgatt
     8701 catctaaaaa gtagttcttc tcactcattg gtgaagcacc gaataaataa gtgatcatcc
     8761 agcgatagcg cataaagttt ctcgctgttt tcaaataaag ttctgttttg aattcggaaa
     8821 aatcttgaaa ttcttcctga tggctgaaca atgttcttag caactcatcg ccaaattcaa
     8881 aattgaaatg aataccgctt accatctgtt ttcgcttccc gtattttttt gctaaataac
     8941 gtcgatacaa gacatcttcg aaatttttta attttgcaat aatgatctct tcgtcttttt
     9001 ccggtaaggc aggtggcata cttaatggcc agatcatctc ttctttcggt atagaacgag
     9061 cagtcacatc ataaacagca gcgagatact gaaataattc gggaatagaa tcggtaacag
     9121 gggtgatcat ctctgtttgt gtttcgctaa aatctgtttg gatatagggg tgataggatc
     9181 gatcgccaaa gattgctgga tgatcggttt ttgctagatt tcctgcaaga tcaacccgct
     9241 gtccttcccg ttctatacca aatcgagctt ggtcgataaa agggcgcgca ttgacatgca
     9301 ataataattg cttaaaattc atcattaagt gacctccaat tcatatgaat ctaattataa
     9361 tcaaaaagtc taagaaaaag aacgaaattg tttgagaacc gcttacaaat aagtataaaa
     9421 atgaaggcat caaaaacaca gctatgttaa aaacaatcat tcaaacaagg agctgcaact
     9481 tttgtcacag ctcctgcttt ttagacatgg gttattcttc ttttagtaat cctttgaaca
     9541 atccaacgac aaaatcattt ggatcaaacg gctccaagtc atcgattcct tcaccaagac
     9601 cgaccaattt cactggtaaa tgcaattcat tacggatagc aagtacgata ccgccctttg
     9661 ctgtaccatc taattttgtc aaaacaagac cggtcacatc tgttgtttct ttgaattgtt
     9721 ttgcttgaac catcgcgttt tgaccagtgg ttgcatctaa tacaagaagt gtttcttggg
     9781 gtgcttctgg taattcccgc tggattactc gttttatttt ttctaattcg ttcatcaagt
     9841 tcactttatt ttgcagtcgg cctgcagtat caactagtaa aacatctgcc tgctctgtct
     9901 ttgctctttc caaagcatcg aatacgacag ctgctggatc gcctcctgca ttgccacgaa
     9961 caacttcaac accagcgcgt tcgccccaga caactaactg gtcgattgct ccagcgcgga
    10021 aggtatctgc tgctgccatc aatacttttt taccttcgtt tttgaactga tgggcaagtt
    10081 taccaatact tgttgttttc cctacaccat taacaccgac aaacaaaata actgttagtc
    10141 cgtcttcttg gatatttagt tcatttactt cattgattcc ttccgcttca tagagatcca
    10201 caagtttctc gatgatcgca ttttggactt ctgccgattt tttcgcattt ctgagtttca
    10261 cttcttgacg taatgcttcg gtgattcgta tcgctgtttc aaaacctaca tctgcaccga
    10321 taagtgtttc ttctaattct tcaaaaaagt cttcatcgac tgttcgaaaa ttggcaaaca
    10381 actcattcat ccgttcacca aatgttttac gcgtcttttc taaacccttg tcgtacttct
    10441 cctgtacttc aacttcttct gttttctctt cgccagtaaa agcacgcttg attttatcaa
    10501 aaaatcccat ttgactcccc ctactctaat acaaattttt ggatcacttt ggctacgcca
    10561 ttttcttcat tgctggctgt cacgaaatct gctgcttctt tgacgaaagg cacagcattc
    10621 tccatcgcta cgcctagtcc agcatattgg atcatcggta aatcattttc ctcatcaccg
    10681 atcgccatga tttcttcttg tttcaaacca agatcttttg ccaataaaga aataccataa
    10741 gcttttgtaa caccttttgg cataaattcc aaaagcatac ttcttgtttt gatgatctca
    10801 tacatttcat ggaattctgc tggtatttct ttgattttag gatctaattc ttcttggtca
    10861 taagcgatta ctgccttatt aagcacaaat tcatctgtaa tatcgcttat atccgctggt
    10921 tggaattgca gcagcttatt caacacattg tacaaggatt gcttccttgg ggcactaggc
    10981 agctgtaaaa ccacattgtc agacaaaaca tctagtggca gatccaattg ctgagccagt
    11041 ttgtataagc gttggatatc tgatacttcc atcactttct tttccatgat ttcaccagta
    11101 tcattttttt gtaccaaacc gccgttgaaa gtgatgctgt agtcaccttc atctaccagt
    11161 cctaattctt gcagataatg cgccatcgca gctaaggggc gtcctgtaca tagaacgatc
    11221 ttgacccctt gctgttttgc ttgtgctaat gcttgtttat tctcatctga aatcagcttt
    11281 tgttcattta ataatgtacc gtccaaatcg attgctacta atttgatcat tttcgttcct
    11341 acactttcgt ttcgttgatt ttcgcctctt cattgatatc ttccatccgt acagaaacga
    11401 tttttgatac accggattcc tgcatcgtca ctccgtacag tacatctgct gcgaccatcg
    11461 ttcctttgcg gtgtgtgact acgataaatt gtgtgtcatc ttggaaatcg ctcaagtaac
    11521 gaccaaaacg tttcacgttg gcttcatcta acgctgcttc tacctcatct aagatacaaa
    11581 acggaacagg acagacacgg ataatggaaa agagcaacgc gatggctgta agtgctcgtt
    11641 ctcctccaga aagcaaactt agactttgca gttttttgcc aggaggttgt acttcaatct
    11701 caatacctgt tttcagcaaa tcagttggtt ccgtcagtac taattcagct ttaccgccgc
    11761 caaacatatt tggaaagaca attttgaatt cttgacggat agcttcaaat acttctttga
    11821 atcttgtccg cacttcatca tccatctcat ccatcgtttc aaacaattgg tttttcgctg
    11881 ctaacagatc atctcgctgt gttgctaaga atgtatgtct ttcactgact tgttcgtatt
    11941 gttcgattga atttaagttt actggtccca gtttttcaat ctgttctttt aaggatgaaa
    12001 cctttgttct actgtcttcg atatcggttg tttcttgata gtcttgggaa gctttctcaa
    12061 atgtcagctg atattctgac tgcagatagg tcaaatgatt atctaaaatc atttcagctc
    12121 ggtctttttg gacttctaat ttggattgtt ccgacagtcg tgctttttgc tcacgattac
    12181 gctctgccaa gactgcttca agcttatcga tctccttttg ccttctatct cgctgctctt
    12241 tggcttttgc caactcggct tttaatgttt ctcgctgagc agataattcg ttaatttgtt
    12301 tttccaaact ttcttctgtg acttcatgat cagaaaagtc tgctgtcaat gtcgccaact
    12361 gcttttctat cgcttcttga cgttcggttg cctctgcttt ttggactcgg gcaccgcgga
    12421 gctggatctg cagatgatta aactgctctt tcaagacagc aagatctgct tgttcttgtg
    12481 ctttttgact ttgtacttgt gctcttctgg cttccatctg gtcgctttct tgacttaacg
    12541 atttgatttc ttcgtcgatt ttttgtcgtt gcgattcaag atctgtttgt ttgtctttca
    12601 attcgttcgt ttgcttttcg taatcttcga taaactgctg cagttcacgt gtctcaaaag
    12661 ttgagatttg cttttctttt tcaaaacgtt caagttcatt tatgatgttt tgtagctgat
    12721 ttgtcgcttc ttgttcttca aaacgtaatt gttcgcctcg tgtacgcaaa acttcttgtt
    12781 cttcggctaa acgagctgtt tcttgttgca gctcctgaac ttttttttct tgtgcttgga
    12841 gttgtttatc tgcttcttca aattcactag tcaactgttt caattcttga ttttgtacaa
    12901 acaaactgcc ttggtttcct cgtttattcg ctccacctgt catggatcct ccggcattca
    12961 tcacatcacc ttctaaggaa accactcgat attgatagcg gatcgtttgc gcgattgcat
    13021 tggcactagt taagtctttg gctaaaagga tcgttcctag caggttttga accacagttt
    13081 ggatttgatc gggataactg acttgttcac tggcaatccc tataaaccct tctacagctg
    13141 ctgcttgtgt caatatatga gccggtaatt gtcttggttt gattgtcgtc agtggaagga
    13201 atgttgctct tcctcctctt tgctgtttca aatacgtgat ggcttgccga gcatcttttt
    13261 cattttcaac gatgacatgc tgggctgccc cacctaaagc tgcttcgata gctaacgtga
    13321 aatctgcagg aacatcgatc agttcagcca ctgctccgac aatacccgaa agctgctgtt
    13381 tatgctgcaa gaccaatcga accccttggt aaaaaccaaa atagttttct tggatttctt
    13441 gaaggctttt cttacgagca cgtagctgct ggacttgatt cattaattga tacattttag
    13501 gctgttcttt tgctaatgct tcttggaact ttttcccgtt ttgttggatc atttctaact
    13561 tctcttttgt ttcagttaaa gatgatttta gctgcgcctg cttttgtgtc aattcttctt
    13621 tttgtaagat caatcggtct actgatgctt ctacttccga ttgcttggct aacgtctgct
    13681 tgctttttgc tgtttcttgt atatattgac gttctagata cttcagttcg tttcctacag
    13741 ctgcttgttc ttgcatcaaa tcaacatatt gatctcttaa ttctgccaat aattctttcg
    13801 ttgatttttg gtatttttct aattcttgct gtgtttttat gagatttgct tcagcttttt
    13861 ggatctctgt ttctttttct gctgctgctt tcatcaagct ttcttgaagc ttttcaaaat
    13921 gtttaacctt tttttgtgct tctgctaatg aagtttgata ttcttgcgag cttttttgtg
    13981 tatgctttgt ccgctcctgc aaaacatctt tttgtccttc tgtctgcttc agtttttccg
    14041 aaagatctaa taatacttgc tgattttttt cgatcagacg atcggcttgc gcattttctt
    14101 tacgctgctt cgccaaaatg gattcttgtt cttggatcga ctcagatagt ttgcctaatt
    14161 ctagattgaa tttcgcaagc tgtgcttgtt tgttgtccca atcttttttt gctgtcttga
    14221 tttcagcaac catcaaagag acatcggttt gagtcagtgt ttcttttagc cgtaaaaact
    14281 cctttgctgc ctcactctgg gcggccaaag gagttaactg ttcttccaat tcgtggatga
    14341 tgtcttggac gcggctgagg ttgtcttctg tttcaaaaag cttctgttct gctttctttt
    14401 tccgctgctt gtatttcaag acaccagctg cttcttcaaa gatcccacga cggtcttccg
    14461 gtttactact gaaaatcgct tctacttttc cttgagaaat gattgagaac gattcttttc
    14521 ctaatccaga atctaagaac aattcttgga tatcttttaa tctgcagctt tgtttattga
    14581 tgaaaaactc gctttcccca gtccgtcgat aacggcgggt tacactgatc tcattgtact
    14641 ccaatggcag ataatgatca ctattgtcta aaatcactgt gacttctgca atattcagct
    14701 gcttacgggt atcggaaccg gcaaagatga tatccggcat ttttccgcct cgcagacttt
    14761 tagcagattg ttctcctaat acccagcgga tagcctctgt aatattgctt tttccgctgc
    14821 catttggtcc aaccactgct gtgacgctat tttcaaaatc aatgactgtc ttgtctgcaa
    14881 atgatttaaa accggctatt tcaattcgtt tcaaatacaa agctttccct cacttacttt
    14941 tacagtgctg ccaatgcatt ttccgccgca gcttgttctg ctaatttttt cgatttccct
    15001 tgtccagtgc caatcaattg atcatctaca taaacttcga tccaaaatac tcgttcatga
    15061 gctggacctt cttcattgat caatcggtat tcgattgata catcacctga tttttgcaag
    15121 acttcttgta gttttgtttt atgatccatc tcatgtgaaa aagcacctgc tttgacttta
    15181 ggaaaaatca ctttttcgat gaatgcatgg gtcgtgtcga atccctggtc aagatataac
    15241 gcgcctaaaa aagcttcgaa taggtcgcaa agcaatgctg gtcgcgtacg tcctcctgaa
    15301 ttctcttctc ccttgcccaa caaaacatat tgatcaaagt gacattcttt ggcaaatttc
    15361 gacaagctgt cttcgcgaac gatcgcagct cgtgtttttg tcaattttcc ttctggcaaa
    15421 tgaggatata aacggaataa atactgagaa accatcagtt ctaaaacggc atctcctaaa
    15481 aattcaagtc gctcattatc cgataattgc agattgcgat gctcattcac ataggatgaa
    15541 tgggtaaaag cttgttcaag caaatttaga tcgtgaaaaa cgatgttata gttttctttt
    15601 agttcttttg tcagctggtt gtccattttc cacattcctt ttatggatat tttttcgata
    15661 cttccttatt atactggttt tttgtcaaat ttgaaatggt tcggtcaaaa ggtaagtaaa
    15721 gctttaataa tcctagtgat tgacgagaag ttgtgaaaaa ttcgtttagt ttcgagtatg
    15781 aagaaaaaat tttgtgaaaa tggctttcca tatttttgca taaatttgac ttaataccga
    15841 gaaactggac tttgaacacc gtttattgga gaagtcatga aatcagcgcc ttgacctgaa
    15901 tattagagaa ataaacagaa aaatatcaaa aaaccgaaaa aggacatcgc cctttttcgg
    15961 tcatttttag tgattatgaa cgtgaatctg ctgtaacaga aacatttgcc catgggtttg
    16021 gtacatcata agaccaatcc cagtctttga cacggtcgtt gactggcaat acttcattac
    16081 ggaacaacgt tggaatcgca aatgcttctt caaatgcata ttgttgccat ttttgatatg
    16141 cttctttttg gtactcttca tcaaatgctt tgcttgaact gatatcgttg atcaattttg
    16201 tattttcttc tgattcataa cgagtgtagt tgaacgcagc atttggtccc catagtccac
    16261 ttggatttgg atcagttcct gtattccaag cagcttggta aacatcaatt tcaggatcat
    16321 cgttttctaa tttatcatag aaagaattga attcgatcag acgacctgtt gtgtattgga
    16381 catccaaacc aatttctttc cattgttgga tatagtaatc tgccaatggt tgcgctgttt
    16441 ccccaccgga catcgaagca aagttgattg ttagtttatt tccatctttg tcttctctta
    16501 aaccatcgtc atccacatct ttgtagccag catcatctaa tagttttttc gctttgtcta
    16561 gatcttgtgt gaatcctttc atctcatcat catggtatga ctggaatact ggaggaatca
    16621 atgaagtcgc attgcttctc aaacctgcat agaaacgttc acctactgca ttattatcta
    16681 atgcatagcc cattgcttga cgtaatgatt tatcagccat ttttgaatct ggattgtatt
    16741 ccacactctt tgtatctgca ttccatttac ctaacttgaa tcctagataa gtataagaag
    16801 tttgttcacg gcctaagttt gtatagccag caatttcttt ataggtatcg taagtatctg
    16861 ttggcatttg gaaaatcata tcatataatt tagagttcaa tgcttcagtt gcacttgcca
    16921 tcggtacaga tttcatcgtg atctttttca atttaggcgt accattccag tagtgctcgt
    16981 ttggcaagta ttctactgat tctcctgcaa cgatattact catgtaataa gggccaaaag
    17041 tcactggatt tttacgtact tgatcgcttg attccatatc tttgattgct acaccttcta
    17101 acgtatgttt tggtgctgca tatgaccaga taccaccacc cgattgaagc atgcttgggt
    17161 ttacttcttt atattcgatt tcaactgttt tgtcgtctat ttttttgatt ccggagattg
    17221 tgtcggcact tccatcatga tattcttcca tacctacgat gttacggaaa gtgtcgtcat
    17281 aacggatacc agtgtaatct ttattcccaa tgatttcgta agggaaaatc acatcgtccg
    17341 ccgttacatc ttcaccatct gaccatttga cgttgtcttt tactttgatt gtcgcttttt
    17401 tcgtatcttc atctagatca agcgttgcag ctccgtcgtc tgtgattctg aaatcgtcat
    17461 cgtttgcaaa tagcggttca tgagaaggag ccataaaagc tgcatcatat gaatcttggt
    17521 aaaactccca ctggaacaat cctttaaact gactgtccgt tgcgaccgca gcttctaatt
    17581 cgccgtcttc gattgcatca tcattatttt ttgttgcaat tggaaaagtt aattcctctt
    17641 tgttattgcc gttgcctcca gagttgtttc cagaggtatc tgtacctccg cccccgcatg
    17701 ctgcaagaac gacagcagac atcagcgctg caaatccaaa aatcgttttc ttcattccta
    17761 tttccccctt cgcttctata ctagcctaac cgttggcgcg catctgcaga acgcttgaat
    17821 gcttgtccaa cataattgat actcaacatc aaaactaaaa taaggattga tgcgggtaac
    17881 cagatccatt gccgattcac taagacttct ccgttacttg cataaccgat caatgtacct
    17941 aaactaggta catttgccgg caagccgaaa cctaagaaag tcaaagttgt ctcgataccg
    18001 atgtttgccg caaagttcat cgtcaaatta gtaatgatta atgaacttag attcggcatg
    18061 atttcgcgga acatgatttt aaaatcattg gttcccattg tttttgaggc actgacataa
    18121 tctcttcttg cttcagataa tgtcttgctt cggaataatc gggctttagc tacccaataa
    18181 aatgcactca tgatgaatat aaaggaccag acattgtatc tggaaataat cgatacgaag
    18241 acgataatga tcagcatgat cggtagaatc atgataaaat caacgatccg catcaagatg
    18301 ttgtcaatga tccctccgta gtagccagag atgatcccta atccaacccc gatgatcgaa
    18361 gtaaggatcg tgatcgcaaa tccgatcgta acagagtttc tagcacctat gatcaattgt
    18421 cctaatacat cacgtccgcc ttcatctgct cccagaatga acttttgtcc ttgtgtcgaa
    18481 actgttcctg gtgcagcgta tttgtcgaag atactaacat acatcacttt gtcttgatcg
    18541 gtcagcatcg caccgataaa gatcactaat aataaaacca ctagcaaacc taatgaaaaa
    18601 atagcaattt tatctttttt aaattctcga gcaatcattc gtatccccat aggaggaata
    18661 ctttcttgaa ctgctgtatt gtttttttca ctcatcgatc cattctcctt tctgattggt
    18721 tttattgaat ccgaatacgc gggtcaacga tactcatgat gatatctgaa agtaatgtcc
    18781 ccactagcgt cgcgattcct aaaatcaata ctaaggatgt gatcacggta tagtcccgtt
    18841 ggccgatgga atcaataaat agttttccga ctcccggata accaaaaatc ttttcgataa
    18901 ctacagaacc agcaatcaac gaagtgattt cataccccat ttgagaagcc atcggcaacg
    18961 ctgcatttcg gaaaatatgt cgactgaata ctttattcgt cggtacccct ttggaacggg
    19021 ctgttctgac aaaatcgagc gatttcgaat cgattacttc acttcgcaga tattggattg
    19081 tgacggcagt cgccaaaaat gcttgggtaa ttgatggtaa cagaatatat ttcaaacggt
    19141 ctatccattg agtgataaat ccaccttgca aacctagtgt ttgtgatcca cttgttggga
    19201 accagcctaa acgataacca aaaacaaata gcatgatcaa gccgaagata aataaaggaa
    19261 cagcaaaact aaagaagttg tagattacga caatcttatc caagattgaa ttttgatacc
    19321 ggccagcaag catccccaga ggcacagcaa tcaaataagt aaggacaact gtcaagatcg
    19381 atagatataa agtgttgatg atccgttcgc cgatcaagtc agcaacagag cgtttgtata
    19441 agaaactttg accaaaatcg ccttgcaaag catttccgat ccaacggaag tactggacat
    19501 accatgggtc attcaagcca gctgcttgtc gcatctgttc gatcactgcc ggatcctgat
    19561 ttggattgat caaaccggta aatggatccc ccggcatcgc ttgggctaat aagaagatca
    19621 gaacacttaa aataataact tgcgggatca tcaataaaac acgtcgcaga atcgttttcc
    19681 acattagctt gcacctccat ctttcaacgc cactttgtgt gtagttgtga tcgtacgcaa
    19741 atcgtagact cgaccagtcg catcatagta ttctttttgg ttttggatat actcttgttc
    19801 tactcttcgt cgattttctt tgtggctttc cctattttct acatcgattt gcggaatagc
    19861 tgaaagcaga cgtttcgtat agatatgtcg cgggtcatta taaatgtctt ctcttgtacc
    19921 gatttctaca aatcgacctt tgtacataat tgcgatattg tcgcacatgt gttttactac
    19981 acctaaatca tgtgaaatga ataaatagct gaggccgtat tcttgctgga ttcgtttcat
    20041 gaaattcaat acttgcgctt gaacagatag atccagagca gatacaggtt catcggctac
    20101 gatcagtttt ggattggtag caacagcacg ggctactcct agacgttgac gttgtccgcc
    20161 tgaaaattca tgaggatatt tatatagcgc atcttccggc atcccaacga tatctagcag
    20221 tccttttacc tttttctttt cttcttgatc gcttaaccgt tcaaaatttc ggatgggttc
    20281 agcaatgata tccaacactc gctttttagg attcaaactg gacattgaat cttggaagat
    20341 catctgcaca tccttgttat aacccatttt tttgcggttg cttcgcttcg ttacatcttt
    20401 tccttcatac atgatttgtc cggctgttac cttttctaat cccacaacgg cttttcccgt
    20461 agtagatttt ccagagccag actctcccac taaaccatat gtctttcctt tttcgataat
    20521 aaaatccaca ccatctacag catatacatg atccgttact cgattgaaga atccactgcg
    20581 aatagggtaa tgaactttca ggtctttgat tgtgattaat tctgtcatct acacctctcc
    20641 tttttcgtct ctaaagtgga aatgcttata gcaggagcaa cgtaccaagt ggttcggtgc
    20701 gacttcatga aggataggct cttcttcatg atcgcttgct ggtatccatg gaatacgtgg
    20761 tgcaaaccga caacctttac gaggcatgtt tttcagagaa ggaacgacac cttcgatcac
    20821 atggagttct gcatcatgat ttccttcttg tggtatagag ttcagtaaag agcgtgtata
    20881 aggatgctta ggatgatcaa acagttcttt tacacctgct acttcaacga actgtccgcc
    20941 atacatcact gccactcgat cagccatttc tgctacgact cctaagtcat gagtgatcaa
    21001 gatgatccct gtttttgttt cttcctgaag gtctttcagt aaatcaagaa tctgtgcttg
    21061 gatggttaca tctagggctg tcgtcggttc atctgcaatg acgattggag gtttgcaggc
    21121 gattgcgatt gcgatgatga cacgttgtct cataccgcct gatagttcat ggggatattg
    21181 gcgtccaact ctcgcaggat taggaatccc tacttgttct agcaattcta gtgcacgagc
    21241 ttgacgctgt tctgcattca tctttgtgtg ataggaaagt ccttctttga tctggtcttc
    21301 gattctcatg agaggattta atgcagacaa cggatcttgg aaaatcattc cgatatcatt
    21361 tccgcggatt ttattataga gcgtttcgtt caggtcgatt aaattcagat cgttatataa
    21421 aatctcacct gtaatttttg tgttattagg atcaagtaat cccataatcg tagtagccaa
    21481 ggtacttttc ccgcacccag attccccaac gattgctaaa atctcgtttt tgtctaaagt
    21541 aaatgaaacg tcatctactg catcgtaata gtcatctttg agtcggaatc ctgtatgtag
    21601 gttctgaact tctaataatt ggttatcgga caatcgaata cccctctctg cttcctaaat
    21661 ggaagttttt cttaataaaa aaggacatta cacagaataa agcgttttat aacaaaggtt
    21721 attcttatc
//
LOCUS       DAZBTF010000049        21713 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000049 DAZBTF010000000
VERSION     DAZBTF010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.049"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            19..540
                     /locus_tag="ACHGNS_001857"
     CDS             19..540
                     /locus_tag="ACHGNS_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN6937792.1"
                     /translation="MHIGDKIHKLRRLNNETMNDLVKQTGLSKSWVSDIESGKKQRAD
                     FNKICAIAKHYKVSLEYFQDDYLDSDEHEDIIPLTDETRVKSVKSLEQSLKYLNNKQE
                     PNLLEIKLKDTDSVPEVYYKGQKLEGLIDVSYHYHTTTDVVAGSTNDINMEYLGEVTK
                     YPDIKTIGHKRDV"
     gene            637..858
                     /locus_tag="ACHGNS_001858"
     CDS             637..858
                     /locus_tag="ACHGNS_001858"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFN6937793.1"
                     /translation="MCQECKRRGRNTRGTIIHHIVEAREDLSLFWSVDNLECICVACH
                     NREHPERSGGKKKPKPKSHIVKMYSTPER"
     gene            complement(1015..1179)
                     /locus_tag="ACHGNS_001859"
     CDS             complement(1015..1179)
                     /locus_tag="ACHGNS_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HFN6937794.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGREVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            1282..1803
                     /locus_tag="ACHGNS_001860"
     CDS             1282..1803
                     /locus_tag="ACHGNS_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFN6937795.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            1790..2230
                     /locus_tag="ACHGNS_001861"
                     /pseudo
     CDS             1790..2230
                     /locus_tag="ACHGNS_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            complement(2270..2428)
                     /locus_tag="ACHGNS_001862"
     CDS             complement(2270..2428)
                     /locus_tag="ACHGNS_001862"
                     /inference="COORDINATES: protein motif:HMM:NF024064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HFN6937796.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            2521..3282
                     /locus_tag="ACHGNS_001863"
     CDS             2521..3282
                     /locus_tag="ACHGNS_001863"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFN6937797.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            3469..4821
                     /locus_tag="ACHGNS_001864"
                     /pseudo
     CDS             3469..4821
                     /locus_tag="ACHGNS_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            4837..6057
                     /locus_tag="ACHGNS_001865"
     CDS             4837..6057
                     /locus_tag="ACHGNS_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFN6937798.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDAEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALIKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGGNEGLKVKFASNVDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGSNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTNSSKSVAENG"
     gene            6092..6769
                     /locus_tag="ACHGNS_001866"
     CDS             6092..6769
                     /locus_tag="ACHGNS_001866"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFN6937799.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGIDCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAETPQEETLLQKKLKA
                     LNGGKNE"
     gene            6766..7962
                     /locus_tag="ACHGNS_001867"
     CDS             6766..7962
                     /locus_tag="ACHGNS_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010905933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFN6937800.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRQFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     INPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSNWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIINVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTSIANE
                     VPVTNG"
     gene            7977..8300
                     /locus_tag="ACHGNS_001868"
     CDS             7977..8300
                     /locus_tag="ACHGNS_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFN6937801.1"
                     /translation="MILDPKMDLDEIKNALKIDTDDDDVEVRRAVQAAIAYIRGAIGN
                     DKPSFYKQENDTVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKARYL
                     LFVEE"
     gene            8304..8639
                     /locus_tag="ACHGNS_001869"
     CDS             8304..8639
                     /locus_tag="ACHGNS_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFN6937802.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVRTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            8636..9031
                     /locus_tag="ACHGNS_001870"
     CDS             8636..9031
                     /locus_tag="ACHGNS_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFN6937803.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEKLQQNT
                     PYWDGTKSNGKRGSYMQEHAKDHVVTSSVKNGLIEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            9028..9399
                     /locus_tag="ACHGNS_001871"
     CDS             9028..9399
                     /locus_tag="ACHGNS_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937804.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EIFGNYEDFASNNPLTIQFNVQVDVWVSTLKEVDAFYFALDEIMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            9415..9975
                     /locus_tag="ACHGNS_001872"
     CDS             9415..9975
                     /locus_tag="ACHGNS_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFN6937805.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGVNVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLAKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEEMTLQKFKDLVNNKAGE"
     gene            10102..10413
                     /locus_tag="ACHGNS_001873"
     CDS             10102..10413
                     /locus_tag="ACHGNS_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937806.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     R"
     gene            10437..10628
                     /locus_tag="ACHGNS_001874"
     CDS             10437..10628
                     /locus_tag="ACHGNS_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937807.1"
                     /translation="MDQIYKDLIESGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            10628..14473
                     /locus_tag="ACHGNS_001875"
     CDS             10628..14473
                     /locus_tag="ACHGNS_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFN6937808.1"
                     /translation="MAGKGQPAGNIKLGISLDSTNFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKKIEDFGDKSTRSARKLDGLKDKLGSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIDKVGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NKVSDAIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGADKLEEWKVPQYAKGTPNGGHPGGPMMVNDGRGAE
                     AVITPNGQAFIPRGRNVVLNAPKGTHVLTAEETAYMTGNKAPRYRYAKGTGFFGNLWN
                     NVKGFAGDVGNKLKDVVGDVWDFVTDPGALARKVLNGLGVLEGLVKYPLDVGKGILSK
                     ATEALTNKITELFSSGSLDTSMGMQGVYKYLADVAVAVMKKFPGFVATSGYRPGDPYS
                     HGKRNAIDIALPGVTGGSPRYTEAANYAFEKFANKIGYVITNGKVRDRSGQSGTGIHN
                     DWRPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTAN
                     LNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFDP
                     LSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAKR
                     SRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKGN
                     SRTDRDLALDIQKILVRRM"
     gene            14474..15346
                     /locus_tag="ACHGNS_001876"
     CDS             14474..15346
                     /locus_tag="ACHGNS_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage distal tail protein domain-containing
                     protein"
                     /protein_id="HFN6937809.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSENVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            15343..16392
                     /locus_tag="ACHGNS_001877"
     CDS             15343..16392
                     /locus_tag="ACHGNS_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937810.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGADEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            16396..17220
                     /locus_tag="ACHGNS_001878"
     CDS             16396..17220
                     /locus_tag="ACHGNS_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937811.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVTSTGSTVSISKISDSYELTLS
                     KGEILWSGTALMHDTQTVQPSKKMWQTVSGFLLVWLPYENGQAIGDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            17238..19370
                     /locus_tag="ACHGNS_001879"
     CDS             17238..19370
                     /locus_tag="ACHGNS_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFN6937812.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKTNDRIDQTNQQIGDLGKLKKMYSNSIDFGDYD
                     YSGNPNLSAKLNASSFSLGTGATVADDNDEIVFTLDGTNQLSKYASKAQVPVAEGKQY
                     TISCEIMLEDGFTGDPSGIRLQHAYLPGGVVVLQTETVPKNELNTWQTLIGTRTVKYT
                     SDVPNEWYPLFRDIRTLKPTGKVRLRKIKIEEGSTATPYQPNLLDDPYWLGKAPLGEN
                     IANKSVTFPIKSSAYSLYQANMEEEFVLGQTYTITMKATKPPIQTFIVYNEDSGDYRY
                     GNLEPVEGLVDTWGLTFTPQKVGVNYPKRLTIIQYPQSTTGACQIDWLKIEKGNTRTP
                     NISEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEI
                     NSQLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSA
                     KLYREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQL
                     SIGADGAIKWRRNSSYGSDYTFAITYTI"
     gene            19387..19536
                     /locus_tag="ACHGNS_001880"
     CDS             19387..19536
                     /locus_tag="ACHGNS_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937813.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPILNWKPKFNY
                     WEGNK"
     gene            19533..19979
                     /locus_tag="ACHGNS_001881"
     CDS             19533..19979
                     /locus_tag="ACHGNS_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937814.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            19981..20118
                     /locus_tag="ACHGNS_001882"
     CDS             19981..20118
                     /locus_tag="ACHGNS_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFN6937815.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIISKEESA
                     S"
     gene            20155..20448
                     /locus_tag="ACHGNS_001883"
     CDS             20155..20448
                     /locus_tag="ACHGNS_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937816.1"
                     /translation="MINLGEWGTIAGSITAIVSLILLVIRPVTASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERSR"
     gene            20471..20668
                     /locus_tag="ACHGNS_001884"
     CDS             20471..20668
                     /locus_tag="ACHGNS_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFN6937817.1"
                     /translation="MILPDKYYQVIKWTVLTVLPALSVLVATLGKAYGWNGTDMAVLT
                     INAAATFLGVITGVSAYNLKK"
     gene            20679..21698
                     /locus_tag="ACHGNS_001885"
     CDS             20679..21698
                     /locus_tag="ACHGNS_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFN6937818.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGVNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVKADGIY
                     RPDVNQAYNTLGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPQR"
BASE COUNT         7542 a         3735 c         4824 g         5612 t
ORIGIN      
        1 gattaaggag gaagagtaat gcatatagga gataagatac ataaattgag acggcttaat
       61 aacgaaacaa tgaacgactt agttaaacaa accggactgt caaaatcatg ggtatctgat
      121 attgagtctg gaaaaaagca acgcgctgac tttaataaga tatgtgcaat agccaaacat
      181 tataaagtta gcttagaata ctttcaggat gattatttgg atagtgatga acacgaggat
      241 attattcctt tgacagacga aacaagagtt aaatcagtga aatcgcttga acaaagccta
      301 aagtatctga ataacaaaca agaacctaac cttctagaga tcaaactaaa agatactgac
      361 tcagtaccag aggtttatta caaaggtcag aagttagagg gattgataga tgtaagctat
      421 cactaccata caacaactga tgtggtagct ggttcaacaa acgacatcaa tatggagtac
      481 ctaggtgaag taactaagta tccagatata aaaacaatcg gacacaagag agatgtgtaa
      541 atgaaagaag ctagacctag agacgagata gacaaactat acaagaccaa acgatggcga
      601 gacctaaggc aagtagtaat agctagggac ttcggcatgt gccaagagtg caagcgtcga
      661 gggcggaaca caaggggcac gatcatccat cacatagtcg aggcgaggga agacctgtca
      721 ctgttctggt ccgtagataa ccttgaatgt atctgtgtag cttgtcacaa cagagagcat
      781 ccagagagat caggcgggaa gaagaaacca aaacctaaat cacatatcgt taaaatgtat
      841 tcaactcctg aaagataagt ttgcagcgaa atgaaggtag cccccctact ctaaaagatt
      901 aaagagtaag gcttgagaag aacggtgctg tccttccttc gtaaaaagac cgcttttcaa
      961 gttttttgga gaaaaaggaa aaagccgacc aatttaagcc ggctttggac gaagctattt
     1021 cttagtccat ttgtttcctt tttgagaagt aggaggtaat cggtcgcctg gatcaatttt
     1081 tacttctcgt ccgccttgga catttccacc acgaggtccc acttctttat aggttccttt
     1141 tggtttattg tcttcgccgg gtttatagag ttctcccata ggaatccctc cctaaaaaaa
     1201 tttcggcaca gcactgccga taacttaatt ataaggattg tgataacgat ttcaatatat
     1261 cttttgaaag aaggtgatat tatgccgcaa ccagcgaaga gtgcaaaatt acaattatta
     1321 aacggaaacc caaataagaa gaataccgaa gaactccgca agcgagcggc cgcagaagac
     1381 aaattaaaaa tggctactga caaaatcaaa ccgccgttat ggctagattc gctaggaaag
     1441 gatacctttg agtttatcgc tgatgaattg ctgtctgtgg atttaatcag taatccggac
     1501 gtccatacaa tggctctcta ctccaattgg tattcgcaat acgtttcttt agaaaaacag
     1561 cttcgaaaac tacaacgaga gtacaagttg aactatgcgc ttgcgaaaaa ggaggcagag
     1621 gcgagaggtg agccgtttaa tgaacctaat gaattaattg gtaatccgct ctctcggcag
     1681 atggatacag cgtcgcggaa tctccgttct tttggcgctg atttaggact atcaccagca
     1741 gccagagcta agttagctat taagatggct gatgatggtg gtgatgacga tgacgacttc
     1801 taatattttg gatatgtcct acacagaacg tgtggactat tggcaaagct atcttgagga
     1861 gcaagcttct tggggtggct ttttaaaatg tccatatccg gaattgttaa ctacttggta
     1921 tgcggaacga ttaatcgatg gaagcatacc agccagcaaa gaaaatattc aagctgctaa
     1981 acggcatatg cgtgatttgc agcgccaagg aacagatgat tttccttgga tctttgacga
     2041 agaaaaaggt caccggccta ttagatatat cgaaaaaaaa tgtaaaccaa ctgaaggcga
     2101 ctttggttcg tttgttttgc aaccttggca gcatttcata attggatcca tgtacggatg
     2161 ggtacatcgt gatacaggag agcgtcgctt ccgcgaggct cttatttttg ttggacgtaa
     2221 aaacggtaag ttttgccgtt taaaaatcgg gcaaaatcgg tgaaaacctt tatttttggc
     2281 tttcttttag taataattcg atagctttat caagcaattt agacatagga accatagttt
     2341 cttccgacat tttttttaat ccttcgtata aatctttatc aattgcgtta gaaattctaa
     2401 ttctgttttt tagtccgtat ttgtccatgt gtgacactcc tttttatttt actataacat
     2461 ccgattccac cgcttgcaac taccatcaat tgatggtagt atataaaaga ggtgagtaga
     2521 ttgaaaaaat acattattta caaacactta aataaaataa atggcaaaat ttatatcggt
     2581 gtcacaaatg atattggcag aaggtggagg agcggtggga ttgaatataa accaccaaag
     2641 aacgaaaccc aacaccacag aagtttttgg aatgcaatac aaaaatacgg gtgggaaaat
     2701 tttgatcatt tgattattga agaagactta acgatgaaag aagcttttga gaaagaaaag
     2761 ttttatattg aactttatga ctcaactaat aagaaaaaag gttataacat cgcaaaaggc
     2821 ggaaatggcg gaataatata taaagttcac cccaaaggaa tgctagggaa aaaacaatct
     2881 aaagaatttt cttcaaatca cagtaaatgg gcaaagaacc ataagaataa ttgtatgaca
     2941 aacggtgatg ttgtatgggg tgttactcac gaacacccaa aaggcatgtt gggaaagcac
     3001 cacagcaaag aatcaattat gaaaaagaaa gcatatagtg gtgaaaatgc tgtaacctca
     3061 aaaccaattg tcgcaattga acgggatgga actaaaagag aatttcacag cgctaagctt
     3121 tgcatggctt actattcgat tagtacatgt gttttttacc gacttttaaa agatggcgcc
     3181 ccttatgtga taaatcctaa agcgaactac agaaataaag aaaaaatatt agctatcgaa
     3241 ggaattatgt tcaaacataa agaagatacc gaggtaagtt agtagattgc gaaaggttac
     3301 taactaccgt aacgcgtagg agttgaataa atataatgct cccaagagtg tccgacagca
     3361 gataatttgc tgaaaatgta cgctaaactg ggccagaact gactgaccga tgaaaatgag
     3421 ggtgacctcc agagtgcgag ataaaaagct cgcagataat aacaatcgaa aacatcacta
     3481 atttcaggcc tttccacata catggtcgct tatgatgatg aacaaggcgc caacgtttac
     3541 gtattggcaa atgctcgtga tcaagcaagc ttgttgtttg ataaggccgc agaaatggtc
     3601 aaacaatcgc cggcgctctt taagaaattt ggtaagccta aacgatcaag tattaattat
     3661 gctcccgcct tttctaaaat ggaaccacgc gcctcagata gccggaaatt ggatgggcta
     3721 aacactcact ttggtatttt tgacgagatc cacgagttta cgaattacaa tctgatcaac
     3781 gttatcaaga aatcaagagg aaccagaaaa cagcctctga tagtttatat cacaactgct
     3841 ggatatgtat tagatggtcc gttgatgtct tattttgagc aaggtgtgga ctgtttggaa
     3901 catttggaag atgacatcga tgaacggact ttctattatc tggcaaaact tgacagtgcg
     3961 gaagaggctg atgacccaag attatggatc aaagccaatc cgaatatttg tctaatgaat
     4021 tttgttggca tgctagatga ctatgttaag gataaaaaag atccaaaaga atatgctgac
     4081 tggattacca agcaatttaa cttgttttcc gatatcgatg agctgtcatt tgtcgatatg
     4141 cctaccatta aacgaaacaa taaaaccatc gatattgaaa cgctcaaagg taagaagtgt
     4201 gtcggtggtt ttgacttgtc cgaaacggaa gactttaccg cagccgtttt agaatttccg
     4261 cttgaaacag gcgaggtatt cattttgcaa cacacatgga tcccacaagc tagatttgat
     4321 cgagataaca atcaagagcg tatcaaagcg tgggagaagg tgggagatct aacgattatt
     4381 cctggtgatt acgtcaatta tgaatacgtc ttaaattggt ttgtagaaaa ttcgaaaatc
     4441 tatgacattg taaaaatcaa ttatgacaag gccaaggcgc tacgattaaa taaagaacta
     4501 gaaaatgcag gatttgaaac caacgagatt cggcaagggt ttctatcatt aggtgggcca
     4561 atgcaaaact tcaaggaaat gctattggac ggtaaggtga ttttcaacaa ttccaagctt
     4621 taccgatggt atctatccaa cgtcaagctg gtgatggatc gcaactcaaa ctggatgccg
     4681 tctaagcagt ccaagagtag aaaaatagat ggttttgcag caagtttgaa cagccacgcc
     4741 gaagtgttga atatgttggt taatcctgtc ggaaccggga aagtaaccta ttactcgatt
     4801 tccgatttaa tgaatatgta agaaaggtgt ggaggaatga gtattttaga tcgtttgcgt
     4861 tcttttggcc gagcgaagcc gaaagcgagc aaacaagagt attttttgaa tgacccggga
     4921 ttgataccgt atttagtcgg aaaagatgaa atatcagaag ggattttttc cgtaattagc
     4981 cgtgtatcga acgtttttgc gtctctccct ctcaaaatga tagatgcgga gtttggccaa
     5041 ccggacgact gtcctgcata caacttgttg agcgaaggcc ctcgatattt tacaaagttt
     5101 gattttttcc gggacgtgga agttttgaga aactaccaag ggaatgcgta tgtgcagatt
     5161 ttccgaaata tcaatggaga agtagcagat atggcgttaa taaaacctgg tgcttgccat
     5221 ccagtgattg atatggatag cggggagctt tactaccaag taactgcgac tgacaaaggc
     5281 agttataagc aagttattta tgtacattac atggaaatgc tccactttaa acaaccgagg
     5341 tttggtggct tggaaggtac agaccccaca aaagtattaa cgaataccct cggatatgat
     5401 cgagaagtcc gaaaaatctc tttaagtcag cttaaaggag gtaatgaagg gctaaaagtt
     5461 aagtttgcta gcaatgtgga tgaagaagct aaaaaagcta cagttaaaaa cattgctgat
     5521 ttttatcgac aaaacggtgg actacttgtg gaagaaaacg gtgtagaaat cgaacgttta
     5581 caacgagagc tggtagacag caagctttta gatactgata aagtatctcg ctccagaatc
     5641 gcgatggtct acaacgtgcc ggaacatttc attgggagta accagtcgag ttactcttca
     5701 caggaacagc tcaatatgga gtttttgaca tacaatctag taccgaccgt taatcaatat
     5761 gaagcggaac taaataagaa aacactatcg agaaccgaaa aagcaaaagg ttatcgatat
     5821 aagtttaatg tcgcaagttt gctaagagct gacactcagg caagggggca attctatcaa
     5881 atcatgcgac gaggcggagc atactcagcg aatgatgtcc gaagatttga ggacttgcaa
     5941 ccgataaata aaaccggtat ggatgattat catatttccg gagacctata tccaatcgat
     6001 atggatccaa cattaagaaa aacaaactcg tctaaaagcg tagccgaaaa cggttaggct
     6061 tttttagttt gcaccgaagg gaggtgaaag gatgaaaaaa gtgacgttaa gcggcgatgt
     6121 cgtggataac gataccgcgt ggttatatga ctggtttggg attgattgta tctcaccagg
     6181 gaaaatttct gccgctctta cagaagcagc gggggatgaa gtagaacttg atatctcatc
     6241 taacggtggg gatgtcctag cggcaagcga aatatatacc gctatccgtg cctatccggg
     6301 aaaggtatcc ggaaatgttg tgagtattgc agcaagcgct gcaagtgtaa tcgcttgtgc
     6361 ttgcgaaccg cttagaatct cgccaacagc acacatcatg attcacaacg catgggtgac
     6421 cactagcggc aacgccgagg aattaaaagc caatgcggaa atgttaagca gtgtggatga
     6481 gtctatcgtt aatgcttacg agatcaagac aggactcgac cggaaaaagc ttgctgattt
     6541 aatggcaaaa gatacttggt taaacgcaca aacagcagtg gcggaaggtt ttgcggatga
     6601 aattatgttt gcagaagcac cagtaacggt actcaatgcc tctcaaccgg ttattccaaa
     6661 aaacgcggta actaagttga aaaatctaat actcaaagcg gaaacaccgc aagaagaaac
     6721 gctcttgcag aaaaaactaa aagccttaaa tggagggaaa aacgaatgaa tttagaacaa
     6781 ttaaaaaatg cgtgggtcga ggcgggaagt aaagtctctg atttaaacgc gcaactcaat
     6841 gcagcattgg ttgacgatga aaaaacagaa gaagatgtag taagtttgca agcacaagta
     6901 aaagcagcac gggctaaacg ggacggattg aaagagcaag tggcaaatat ggaagccgaa
     6961 caagtcttaa acgtcaaaaa agaaccatta gataaaaaag atgaaaactt gaaaaacaag
     7021 tttatcaaag actttaaagc gatggtcaat ggtgatcctg ctattatggc tactttgaca
     7081 tctaatacgg atgaatctgg taatgctatc ggattgacta ttcctgtaga tgtgcaaacg
     7141 actattcata ctttggttcg tcagtttgac tctttgcaag aatacgtaaa cgttgaaaaa
     7201 gtgaccacta ctagcggttc tcgggtttat gaaaaatggt ctgatattaa cccactgacc
     7261 gctttggata ctgaagacgg tgaaatccca gcaaatgatg atcctgcact ttacttgatc
     7321 aaatacttga tcaaacgcta cgcaggtatt tctacagtaa ctaacagctt gctaaaagat
     7381 actgccgaaa acattttggc atggttgtct aactggatcg cgaaaaaagt agttgttact
     7441 cgcaatacaa aaatcttggc agctattgat ggaatcaaag cagcgcaaaa gaaagatgtt
     7501 acagatgttg atgggattaa agatatcata aacgtccaac ttgatccagc tatcgaagct
     7561 acatctatgt ttattacaaa ccaagatggc ttcaatgttt tagataaagt gaaacgtgct
     7621 gatggatctt acttgttaca aaaagacgta gcttctgcaa ctggatatac cttcttaggt
     7681 aaaccaatca agaaaattgc ttctcgtttc ttgccaaaca aaggtacgca agctactcct
     7741 aaatatccac tgtatattgg tgatctgaaa gaagctgtta cattgtatga tcgcgaaaac
     7801 atgagcttgc tgacaacgaa tattggcggg ggggcttttg aaacagacac caccaaagta
     7861 cgggttatcg atcgatttga tgtgcagtta gtcgatgatg aagcagttgt tttagctaca
     7921 tttacatcta tcgcaaacga agtgccagta acaaacggtt aaggagctga tttcttatga
     7981 ttcttgatcc taaaatggat ttagacgaaa tcaaaaacgc actaaagatt gataccgatg
     8041 atgacgatgt ggaagtaaga cgtgcggtgc aagctgcaat tgcatacatt agaggggcta
     8101 tcgggaatga taagccctct ttttataagc aagaaaatga cacggttgat ctgattaatt
     8161 tagctattct gcaattagca gatcattatt acaacgctcg atcagcaacc gtgagtggga
     8221 acttgcgaga gtacgattta ggttttacaa gcctaatctt gcaactcaaa gcaagatatt
     8281 tgctttttgt ggaggagtag cgtatgcccc ttattcaaac aggaaattta aatcaacgca
     8341 tcaagtttgt ccgagatacg acggtcaagg acgaggacgg gcaagttgtc cggacttcta
     8401 caaccattct tacttgctgg gcaagtgtgc agacacaacg cctgaacgat attaagacgt
     8461 ccattggtac ggctttggaa ggaacactga cgttcattat ccgctatcaa caaaaatcag
     8521 agctaaccaa tgatatgaaa gtgcgttgga atggaaaaac gtttgaaatc attacgatta
     8581 cgaaaggcga gtttgcgaag gacttcacga caatcattgc aaaagaggtt tcaaaatgag
     8641 tgtagaagtc gatgcaaccg aagtgtacaa agcgcttagg gaagtaaaag caaacgttca
     8701 acgagtggaa agcccagcac ttagaaaagc tggggagtac gctcaagaaa agttacaaca
     8761 aaacacacct tactgggatg gaacgaagtc aaacggtaaa cgtggttcgt atatgcaaga
     8821 acatgctaag gaccatgtgg ttacaagctc ggtaaaaaac ggattgatag aagtcggcta
     8881 tgataaagat gtttcttggc ggatgcactt tatcgagttc ggaacaatca aacaacgtcc
     8941 aaaaggtttc gtacaaaaaa cacaaaagca aatcgaaaaa caagtaacac aaatcattgc
     9001 tgacgaagta aaaaggaggc taggactttg aaaacggcag tatcacaagt ctattcaatt
     9061 ctgaatagca atgaaaaaac aaagaacatt gatttttaca ccaatagtgt tccggaatcg
     9121 gctcaaacag tacctagcct tccagttggc agaattacag agatattcgg caactatgaa
     9181 gatttcgcaa gcaacaatcc tttgaccatt caatttaacg tacaggtaga tgtatgggta
     9241 tcgaccttaa aagaggttga tgccttttat tttgcccttg atgagattat gagggggaat
     9301 ggttggcaat gcgcatacac ggaacaaaca gatgatgagg atttagaagg tgcaaagcgg
     9361 attatcaaac gatatgtagc aaatatttca ctaaattaaa aggagagaaa atagatggca
     9421 acagtaggat ttgagagcgt catttttggc gtaaaaacag gtgcaggcgg cactctaaaa
     9481 gaattagtag cagataagtc gaaaggcgga gcgatcgaag ctaaaattac tggattaggc
     9541 gcaacttcta acacaacata cgcttcaaac gtaccattct tcattgcaag taaaggggtt
     9601 tcgtcgccaa aagttacgct tgacgtggca gacttaatgg ataacggcat ttacagcgaa
     9661 atcattggcg ctaaaaccgt ggatggtgta aatgtaattg gttcagaaac tgaagcgcct
     9721 tacgtgtcgg tagtcatggt tacagcgaac aaagaaggaa aacgcttatt catgggattg
     9781 gcaaaaggaa aattcagtca tccagatatc gacatgaaaa cagctgaaga caaaggagtt
     9841 gaattgcaaa ccgattctat cgaaggggaa ttcatttctg atgaacgtgg ctatgtatac
     9901 ttaacagccg tagaatcaga agaaatgacc ttacaaaaat tcaaggactt ggtaaataac
     9961 aaagcggggg agtagttaac cctgcatcta caccaacgac agatacaggg acaccaaaag
    10021 aaccagaacc aaaaattgat acacaaggtt agccattttt tggctagcct tatttttttg
    10081 taaaaacaag gaggaaaaca aatgattgaa ttgcaattga aacttgacgg aaagaaaaaa
    10141 acattcaaac aacaagatat ttccgcacgt gcaatgcgtg agtgtatcaa attttacgag
    10201 aaagcggaaa aagcagacct aactgattta gaagcaattg attcaatgat tgcaattaca
    10261 gcagatattt tccaagatcc agcagttaca tttgatgcta ttttagacgg tttgactgcg
    10321 agcgagttag taccggcatt agaaagtgtt tttgaacaaa tcaatgaact gggaaacaat
    10381 gaaaaaaagc agacggcgag caaaaagaga taagtttttc tgaagctagg aaagcaatgg
    10441 atcaaatcta caaagattta atcgaatcag gttggacgat gagagatgtg gacgaagccg
    10501 actatcatta tttgttacac ctttttggag aagtggagag tggcgaagaa tatgtagatg
    10561 gtgctgattt catcaaacaa tttttatcgg ctgaagactt ggtaaaactt gaggaaggag
    10621 gtaaataatg gcaggaaaag gacaaccggc aggaaatatc aagctaggga ttagtttaga
    10681 tagcactaat tttggtaaca cgctggacga aatcaatgcg aaagtcaaac aagctgagtc
    10741 gaatatgcgt gccaatctaa aggcttatga ttcagcagga cgttcatacg aagcacttag
    10801 tcaaaagacg aaagacttgt ctacggttat ggaagggcaa aacgccaaag taagagaatt
    10861 aacaaagcgc cgtgatgaag cgattagcaa gtatggcgag gaatcgaaac aagttgctaa
    10921 ccttaacaca cagataaaca atgctaccgc aaaatataat gcttacagtc gccagttgaa
    10981 tgacacaaaa aaagaattgg tgtattccaa aacagccgtc aatgatttat ctaacgaaat
    11041 caaagaaaat gaacgacaaa tgaacgccga agtaaaagcg ttgaaagccg ctggtgatga
    11101 atctggtgcg tttgaagcaa aacaaaaagg gctagccaaa caaacggaat tatccgagaa
    11161 agctatcgaa gaacagcgca aagttgtgaa actgatggct gatgagtttg gcgattcagc
    11221 aaatgaaacc gaagatgcaa aaagggcatt agaaaagtta gaacgacaaa gccaaatatc
    11281 tagcaggcaa ttagaagcac tcaaaagctc cagcgatcaa tcaggaaaaa aaatagaaga
    11341 ttttggcgac aagtccacaa ggtcagctag gaaactggat gggctaaaag acaaattagg
    11401 ctcgctaaaa agcgcatttt cgtttggtgc agttgctgga ttagcgcata acgctattag
    11461 cagtgtagta agtggcgtgc aaggcttggt tggcgaagca gtaaacgcat cggattcatt
    11521 gatgaagttt tccaaaacca tggagtttgc taactttggg aagtcacaga tagaaagctc
    11581 gaaaaaagaa atgaaagact acgccgataa gacggtttat ggtttagaag aaattctgaa
    11641 cacaaccgca caattggcat ctaacgggat tcctaactat acagaactaa ccaaggcggc
    11701 aggtaacttg aatgccgttg caggcggttc tagtgataca ttcaaatccg ttgccatgat
    11761 gctaacgcag acggcaggag ctgggaaact aacaactgaa aactggaatc aattagcaga
    11821 tgcgataccg ggcgcttcag ggctgttaca agacgctatg ttgaaaaacg gagcttatac
    11881 aggaaacttc cgtgatgcaa tggcgcaagg tcaaatcact tctgatgagt ttaaccaagc
    11941 gattgtacag ttaggtatga atgacggagc agttaaagca gctacttcca cagatacatt
    12001 gagtggttct tgggaacaga tgaaatctac tgtaataaat gggttacaaa gtatcataga
    12061 caaagtaggc gttgaaaata tcactggttt catcaacaga gtaacaaaag ggattgaaaa
    12121 ttctattcct aaaattactc aatttatagg ttggttgaga gatattggaa cgtggatcgt
    12181 tgaaaataga gagccactaa catggattgt cggaatcata ggcggaatta cattagcagt
    12241 aaaagcattg aacgtagcaa gtatgttgct ggcaattact ggcggaacat tggcagcccc
    12301 ttttgtggcg attggtgtag cattaggcgc actagcaggt gctttggtga tagcttatac
    12361 aaaatctgaa acatttagaa atatagtcaa tgcggctttt acagctgtga aaaacgtagt
    12421 tatgagcgtt atcaataatt tggtggaata ctacaaaatg ttgtggagcg tgttgcagtg
    12481 gctttgggaa aaaataaaag aatgggcttc atggattggc aataaattca ttgaaatgaa
    12541 gaacagcgtt gtgaatacgg ttcagaacct atggaatggt gtgaaaaact tcttcagtaa
    12601 tggcgttgga gacacttgga ataaggtagt tggctgggta aaaaacattt tcaacaaagc
    12661 aactgaattg aagaacaaag tttctgatgc aattggtaac ctgtggaacg gtatcaaaga
    12721 cacattccgt agaggcattg atacggtatt caattggttt tcagaactac cgacgaagat
    12781 gaagaatgcc attattggcg gtaaaaatgc cattgttgat gcgttcaaaa gtattttcaa
    12841 cgcagcactt aaagcgatag gtaaaccagt taacgcaatc atccatggag cttcatgggt
    12901 actagaaaaa ctgggtgctg acaaactcga agaatggaaa gtgccacaat acgcaaaagg
    12961 aacaccaaac ggaggtcatc cgggcgggcc tatgatggta aatgacggta gaggtgctga
    13021 agcggtaatc acacctaacg gacaagcatt tatcccacga gggcgaaatg tagtgttgaa
    13081 tgcaccaaaa ggcacacacg ttctaacagc tgaagaaaca gcttatatga ctggaaacaa
    13141 agcaccaaga tatagatacg ccaaaggtac aggctttttc ggaaatctat ggaacaacgt
    13201 caaaggattt gctggagatg ttggaaacaa gctgaaagat gtagtcggcg atgtatggga
    13261 ttttgtaaca gacccgggag cgttggctag gaaagtgtta aatggtcttg gcgtactgga
    13321 agggcttgtc aaatatcctt tagatgttgg taaaggtatt ctaagcaagg ctaccgaagc
    13381 attgacgaac aaaatcacag aactattcag cagtggcagt ttagacactt caatgggcat
    13441 gcaaggcgtt tacaaatact tggcggacgt tgcagttgca gtaatgaaga agtttccagg
    13501 ctttgtggca actagtgggt atagaccagg tgacccctat tcgcatggta aacgtaatgc
    13561 cattgatatt gcactacctg gtgtcacagg aggctcgcca cgctacacag aagcagccaa
    13621 ttacgcattt gagaagtttg caaacaaaat cggctatgtt atcacaaatg gcaaggttcg
    13681 tgaccgttca ggacaatcag gtacaggtat tcacaatgat tggagaccat ggcccgatgg
    13741 agatcactat gatcatgtgc atttaaacgg tgtgaaagac ccacaaaaca ctcaaatttc
    13801 aggagatagc gtgggaggca gtggggtaga aagatggcgc aatgtagcaa ttagagcgtt
    13861 gaaaatgacc ggtcaataca gtactgcaaa cttaaatgca ttactaaatc aaatgcgtac
    13921 agagtcaaat ggtaatccta atgcagttaa caattgggat attaacgcca aaaatggaac
    13981 accatcaaaa gggttgctcc aagtgattga cccaacattc agacagtatg caatgccagg
    14041 attcaacagc aatatttttg acccactatc taacatctta gcttcaatca gatacgcact
    14101 atcaagatat ggctcactaa caaatgccta tcgtggagtt ggttacgcaa acggtggaat
    14161 tgtaaaccaa catcaaattg cggaaatcgc agaaggaaac aagccagaaa ttattattcc
    14221 tttagataag gctaaacgat caagagcgat gcaattattg tctatcgcaa tggacaaact
    14281 aggagtttct cctaaaactt atggaaatgc gacaactgtt tccagtgatt acgaaatcct
    14341 agaagccatt gaaaaacagg cggcaacaac gaatcagctg ttatcgttat tgctggcatt
    14401 tttcaaagga aatagccgaa ctgatagaga tttagcttta gatattcaga agatcttggt
    14461 taggaggatg tagatgcgaa ctgtattact gagaaataga ataaacgaag aaattgattt
    14521 gtctacggag gattattttg ctactgggtt gagtaatatg gggtttgaag taaaaaaaga
    14581 acatgtggga cagtggggga atttcagaga aagcagtgag aacgttgaaa tatctgaatt
    14641 tcagtcatct gtaatcattt ctgtgcatgg gtttcgagaa aaagaactgt acaattcact
    14701 tgtgcagttc ctatcggaag gtccgtttga actggaattt gcttttgacg gcgaaacaat
    14761 ggtaagaaga tgcagtctaa aatctttaag taagactgaa atcgatccga aaacatcgtt
    14821 actaacagac actttagaac tatatttcac atcaaactgg tattccgtaa aacgcgaaaa
    14881 attaattcag cgaccgaatg tagtgaaaac acgtggtaag gtttttccgt ataaaagatc
    14941 ttatatctac actcaaaact tgtgggagaa aaaaggtgta ttcaaattca ataataattc
    15001 tgtataccta acgaatagca aggaacggat gtccccgcta aaaattcgtg tgattgggaa
    15061 gtgttcaaat ccgtattggg aagtaatcca aaactcacaa atcatcgcaa cggatggata
    15121 cttcatagat atgactgaaa cacagactct agaagtatca agcctttttg aagatacaac
    15181 ggcgatttta aaagatattg caggggtaga atcttctgtc taccaacaac aggattatac
    15241 aaaaactaat tttgttcagg ccccgactgg agaattcagc attgtgtttc atgttggggg
    15301 ggcagacgta gagattgagt tatacgagga gcgtgatctg ttttgatttt agcagtcacg
    15361 ctttttcatc gtgatttaat gctttataac gaacatttat tctctaacag ttttgaattt
    15421 ggtgcggacg agatcaacga agaggctagt agctttacga tggataagta cgttcctgta
    15481 aaaacaggcg attttctact agcaaaatat attcctagtg gaaaatttgc atattttggt
    15541 gtaattacgt cgcaggaaga cgaaaaaatc agttgtaaaa gcttacttag tttagctgat
    15601 agtgagatac cgactgcacg tgtgtcagga gataactacg aagagcatat ccggcggttg
    15661 attgaatact atttgctgaa tgatccaacg aaacaactaa aagatatttt agacgtcaaa
    15721 gcagaaagtg cgacctctca ttcgtatcaa gctaccgata cgaataaaca caagttaagt
    15781 gcgtatattc tcaatggttt caaaaaatac aacgtaaaat ggtatttcaa gggaattcaa
    15841 aacagaaaaa tctatacagg catacgtgct gtaaatgaat caatttacat taaagacaat
    15901 tcttctgaat ttagcgattg ggatgtgttt gttcaggcgc cgggcgctgg aaacgagaac
    15961 aagctattaa tcgttgataa agcaatgaaa gatatagaga aaccgataat actgtcaaca
    16021 tggtatttgg acgaagaaaa caatttgaca caagatggat cgaaagaaaa tatcacgaaa
    16081 ccaactgtca atttggttaa tatctacgat caaaccgcag aagataaagc atcctacgaa
    16141 gatgtggcaa aatcagagtt gaaaggcaat acgtattcgc atgaaatcaa agtgaacgtt
    16201 gtaagaaatg caaaaaattt gaatgtcgaa acgattgaaa cagggatgtt tgccacgatt
    16261 tcttataaag gaaagatata caagtcggtt ttgacagctt ggcgaatatc aagtgataag
    16321 gagtttgtgg aattgacttt cggaaacata agaagtcgtt ttatggatta ttttgaagat
    16381 aatgggggat aaaaaatggt tagcaatgtg gatggatatc aatttgaaaa cgtgaaagta
    16441 agcgcagaaa atgatgctag actttatcac gttttatata atcggaaaaa tcaggttatt
    16501 gatggttacg atcagtctat gaatttatct tcaagcggat taacagtaaa agttgctgca
    16561 ggagcagcga ttattcaagg tcgtatggtc gttgttcgac aagaagaaag tataacagtt
    16621 ccagcaaact caagtggtta tatagcatta acagtggatt tgacacaaga agttatacct
    16681 ggatctattc ttccagaatc ggaagaatat gaatggacta ataatcaagt caagctagaa
    16741 tttataacaa aagttataaa aggtaattta aacaatggtg ataaggtcta taatttacca
    16801 ctatgctcag ttacttctac tggatcaact gtttcaatct caaagatatc ggatagttac
    16861 gagctgactc tctctaaagg agaaattttg tggagtggga ctgcgttaat gcatgatact
    16921 caaactgtcc aaccttcaaa aaaaatgtgg cagacagttt caggtttttt attagtatgg
    16981 ctcccatacg aaaacgggca agcaattggg gatagatatg taactacgcc tttttataaa
    17041 gagcgtgtaa cttttactaa tgttttagga gaaattgttt caggatttga tgactatcac
    17101 aaaaagtggt ttagtaaacg aataaactat aattcgaata ctaatatatt tacaggtgca
    17161 gcaagcaatg caagtggaga taacgcaaat atggtgctta ggtatatagt ttctttttag
    17221 ttaggaggta tggaagagtg gcaaatttag aaattaaatt atctgcaaat aagagacagc
    17281 cttatctacg tcaccgtgtt gttggtagag ttggtgatgg ggggctcaca acaatcaatg
    17341 tacaacttct tgaggaagat gaaattacac cttttgtaat taatctaaac ggtactttga
    17401 aatttgtggg cgaagtttca aacggtaact ataccgaggg agaaccagaa ataatcgatt
    17461 cgactaatgg gttaattagt tacacgttca ctaagtcaaa tttcagcacg agtcatcaat
    17521 tcaaacaagc atattttgaa tatgtagatc ctaacggcaa aaaagtaact tttcagaact
    17581 tcatcataga cgtgttagaa cgagtagata ttaattcgga gcaagcgaaa tactatattt
    17641 cttcattgga aaaattacag agtgaaatgc aaaccacttt caatcagttc attagtgata
    17701 aacaggtcca atacgatcaa atctactcga aatataacga attagtaaga ttgataaatg
    17761 aatcagataa gcaagtaaat gatcgtattg ataagacaaa tgatcgtatt gatcaaacca
    17821 atcagcaaat cggcgatctc ggcaagctga aaaagatgta cagtaacagc atcgacttcg
    17881 gggactatga ttatagtggg aatccgaacc ttagcgctaa acttaatgct tcaagttttt
    17941 ctttaggtac tggcgcaaca gtcgcggatg ataatgatga gattgtgttt accttagatg
    18001 gcacaaatca actatcgaag tacgctagca aagcgcaagt gccggtagca gaagggaaac
    18061 aatatacgat tagttgtgaa attatgttag aagatgggtt cactggcgac ccttcaggaa
    18121 tacgtctgca acacgcatat ttgccaggcg gggttgttgt tttacagaca gagacagtac
    18181 ctaaaaatga attgaatacg tggcagacgc ttatcggaac gcgaacggta aaatacacct
    18241 ctgacgtacc aaatgaatgg taccctttgt ttagagacat ccgaacactt aaaccgaccg
    18301 gtaaagttag actaagaaaa attaagattg aagaaggctc aacagccacc ccatatcagc
    18361 caaacttact tgatgaccct tactggctag gtaaagcgcc tttgggtgag aatattgcta
    18421 ataagtctgt tacgtttcca atcaaatcta gcgcctacag cctataccaa gctaacatgg
    18481 aagaagaatt tgttttaggt caaacctata caattaccat gaaggcaacc aaaccaccta
    18541 tccaaacgtt cattgtttac aacgaggata gtggagatta tagatatggt aacttagagc
    18601 cagtagaagg gttagttgat acatggggtc tgacttttac accgcagaag gttggagtta
    18661 actaccccaa acggttaact attatccagt atccccaatc aacaacaggt gcatgtcaaa
    18721 ttgactggct caagatcgaa aaaggcaata ctcgaacccc gaatattagt gagtataaat
    18781 atcgtggtac tggtatgcgt gattcaaaca atccaaaaga ttatgtttgg gatctagcac
    18841 cagaatatgt cgaagataat ttggccacag atattaaaat ttctgaaatt actggtaaag
    18901 caaacaatta taccgatggg aaagtatcgg agattaattc gcagttgact gcttcaatta
    18961 atgaagtaga caccacagct aaggatgctc aaacaaaagc gaatgctaat gcgactgcta
    19021 tagatgaatt agacaataag atcgatgaac gcattaatga tacagctact accacattaa
    19081 cagttacaaa cgggaatacc ggatcagcaa agctttatcg tgaaggaaaa acagtttcta
    19141 tatattttgt ggctttaaac ggaaaaagca gtggtggaaa tgattcaacg atactaacaa
    19201 ttccagaagg ctatcggcca ccaattagtt ttgagcaact ggttggctcg atagaccgtt
    19261 ctactttgaa cagtgctcag ttatctattg gtgcagatgg agccattaaa tggcgaagaa
    19321 actcaagtta tggatcggat tatacctttg caattactta cacgatttag aaaagcgtga
    19381 atcgatatga aggcagcata tagaccaatt gaaccttacg gattcgagca aatcattgtg
    19441 aatgatgaag aacatttacc ggaagaatgc acagaagtcg aaccaccgat tctaaattgg
    19501 aaaccgaaat tcaattactg ggagggaaat aaatgaaaaa catttggaaa tatggacgta
    19561 ctggcggaga gtacgcagga aaagtattgg acgacatgct tgtatccgtt ccttacacag
    19621 atcagccacc gcttgaagga attcgtgctg atggcgaacc gttaacaatc gctgatcaaa
    19681 tgtttgatcc taaattgaat caatggatta ttttagcgaa cgcactagat cacaacgatt
    19741 taaacaatct caaagcaatg tatgagtcgt tagaaaatga gaacggcgat ttaaaacaga
    19801 tcaatgccaa actcatgcta agcgatgtag caattaaaca ggaaaatact gcattgaaag
    19861 aaaaagcgga tagtttagca caaatcaatt caaaaatgat gcttgcttcg ttacaaaata
    19921 gcaaagacat ttcagaaatt aaagagcaac taaatccagc ttcaaaggga ggtgagtagt
    19981 atgtttagtt ttagcgatgt gaaaatgatg tatgattggg gctgttttac tgacgatcaa
    20041 gttcgactat tcgttccact atgcattaca gacgaagaag cagataaaat cattagcaaa
    20101 gaagaaagcg catcttaagt gatgcgtttt tatttaaggt aaaggagttg tcacatgatc
    20161 aatttagggg aatggggaac aatcgcagga tcaattactg cgattgtttc tttgatttta
    20221 ttagtaataa gaccagtcac tgcatctttc tcgaagatta ctgaaactct ttcaaaagta
    20281 agtcacaatt tagatttgct gactaaagat ttagaatcga gcaaatcaga tcgattgatg
    20341 attcatgaag aactaaagaa acacgatgaa agattagata cacatgcaga aaaattggta
    20401 gaacacacac aacaaattaa aactttattt agagaaaggt ctcggtaaaa aagaaaggag
    20461 ttaagaagaa atgattttac ccgataagta ttatcaagtc attaaatgga cagttttaac
    20521 agtattgcca gctttatctg tattagtagc cacattaggc aaagcatatg gatggaatgg
    20581 aacagatatg gcagttctga ctatcaatgc agcagcaaca tttttaggtg ttatcactgg
    20641 tgtgtcggct tataatttga aaaaatagga ggaaacaaat gaaaaagaaa attactatta
    20701 ctgcgatgag cctattaacg gctctttttt tattgccgat taatgggttt gcctatacga
    20761 ttaacaatga atttaattta ggtgtaaacg aaggtagttc tcaagtagca aataatcagt
    20821 acattttact gcatgaaacg gctaatgaaa cagcaacagg acgcaatgaa gcgcagtata
    20881 tgcaacgttc atggactagc gcttatactg cttatattgt gggagacggc ggaattgttt
    20941 atcaagtcgg tcaacctggt tatgtacagt acggtgctgg ttcgtatgct aatgcaaata
    21001 gtcctgtgca gattgagtta caacacacac atgataaagc aacgtttgag aaaaactaca
    21061 aggcatacgt tgaattagct agagattcag caatgaaata tggtattcca cttacactag
    21121 acacaccgta taatcaacca gggatcaaat cgcatttatg ggtaacacaa aatatttggg
    21181 gcgatcatac agatccttac ggttatcttt ctgaaatggg cgtaagtaaa gaaaaattag
    21241 cctatgattt ggctcatgga tttaccgatg aaaatccgac aacttcagat gataaaccag
    21301 tcattgatcc aactcgagca ggtgctgcaa atcctaccct gacagatgga acaaattacg
    21361 cccacattga tcagttcgga gaaatcgaaa acgcaaactt acatgtggct ggttggcaca
    21421 tcgctaacta taaatacgag tatatcttca ttatggatta caatactggg aaagaattag
    21481 ctcgagtaaa agctgatgga atatatagac cagatgtaaa tcaagcttat aatactttag
    21541 gaaatgttgg ctatcatgta tcttttaaca tgcgcaactt ccctaataag aaagtctatg
    21601 taatgatgcg ggcaacgaat gatccagaag ggaacactaa aggcggagca caagattttc
    21661 atgataaacg ctggtattta aatattccgc aacgataaaa atagcccctc gtt
//
LOCUS       DAZBTF010000050        21078 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000050 DAZBTF010000000
VERSION     DAZBTF010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.050"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            67..753
                     /locus_tag="ACHGNS_001886"
     CDS             67..753
                     /locus_tag="ACHGNS_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFN6937819.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            755..1699
                     /locus_tag="ACHGNS_001887"
     CDS             755..1699
                     /locus_tag="ACHGNS_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFN6937820.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            1898..2542
                     /gene="pcp"
                     /locus_tag="ACHGNS_001888"
     CDS             1898..2542
                     /gene="pcp"
                     /locus_tag="ACHGNS_001888"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFN6937821.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            2621..3898
                     /locus_tag="ACHGNS_001889"
     CDS             2621..3898
                     /locus_tag="ACHGNS_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937822.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(4082..4831)
                     /gene="pnuC"
                     /locus_tag="ACHGNS_001890"
     CDS             complement(4082..4831)
                     /gene="pnuC"
                     /locus_tag="ACHGNS_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFN6937823.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            5161..5541
                     /locus_tag="ACHGNS_001891"
     CDS             5161..5541
                     /locus_tag="ACHGNS_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN6937824.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            5637..6146
                     /locus_tag="ACHGNS_001892"
     CDS             5637..6146
                     /locus_tag="ACHGNS_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937825.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            6223..6402
                     /locus_tag="ACHGNS_001893"
     CDS             6223..6402
                     /locus_tag="ACHGNS_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937826.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            6457..7020
                     /locus_tag="ACHGNS_001894"
     CDS             6457..7020
                     /locus_tag="ACHGNS_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6937827.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(7067..7582)
                     /locus_tag="ACHGNS_001895"
     CDS             complement(7067..7582)
                     /locus_tag="ACHGNS_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFN6937828.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(7608..8477)
                     /locus_tag="ACHGNS_001896"
     CDS             complement(7608..8477)
                     /locus_tag="ACHGNS_001896"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN6937829.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8603..9283
                     /locus_tag="ACHGNS_001897"
     CDS             8603..9283
                     /locus_tag="ACHGNS_001897"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFN6937830.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            9436..10416
                     /gene="pta"
                     /locus_tag="ACHGNS_001898"
     CDS             9436..10416
                     /gene="pta"
                     /locus_tag="ACHGNS_001898"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFN6937831.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            10539..11012
                     /gene="tsaE"
                     /locus_tag="ACHGNS_001899"
     CDS             10539..11012
                     /gene="tsaE"
                     /locus_tag="ACHGNS_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFN6937832.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            11005..11529
                     /locus_tag="ACHGNS_001900"
     CDS             11005..11529
                     /locus_tag="ACHGNS_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFN6937833.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(12019..12552)
                     /locus_tag="ACHGNS_001901"
     CDS             complement(12019..12552)
                     /locus_tag="ACHGNS_001901"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFN6937834.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(13416..14168)
                     /locus_tag="ACHGNS_001902"
     CDS             complement(13416..14168)
                     /locus_tag="ACHGNS_001902"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFN6937835.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            14281..15201
                     /locus_tag="ACHGNS_001903"
     CDS             14281..15201
                     /locus_tag="ACHGNS_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN6937836.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            15274..16209
                     /gene="murB"
                     /locus_tag="ACHGNS_001904"
     CDS             15274..16209
                     /gene="murB"
                     /locus_tag="ACHGNS_001904"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFN6937837.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            16507..17985
                     /locus_tag="ACHGNS_001905"
     CDS             16507..17985
                     /locus_tag="ACHGNS_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6937838.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVSICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            18262..20322
                     /locus_tag="ACHGNS_001906"
     CDS             18262..20322
                     /locus_tag="ACHGNS_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFN6937839.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            20405..20974
                     /locus_tag="ACHGNS_001907"
     CDS             20405..20974
                     /locus_tag="ACHGNS_001907"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6937840.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKSDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
BASE COUNT         7086 a         3658 c         4255 g         6079 t
ORIGIN      
        1 aactatattt tttatgtgtt ttgctttata attaaaaaaa tattgtcatt ttatggagga
       61 aaatatatgg aaaattatct tagcctttta ggcgtcttga ttgttattct cgggtttgca
      121 tttaaattag actcgatatt gatcgtaatg gttgctttag ttgttacagc tttgactggc
      181 ggtcttggca ttcaaggaat gttagaactt ttaggtacaa gttttgtaaa caatcgcggg
      241 atggcgattt ttatcatcat tatgttggca acaggaacgc ttgaaagaaa tggtctgaaa
      301 gaatcagcgg cttccttgat caagcgattc aagaaagtat ctgctggtat gattgtagat
      361 atttatggga tctttcgaat gatctttgcg gcttttaacg tgagttttgg tggagttgct
      421 ggatttgttc gtccaatcat tttaccgatg gcattgggga ccatcgaatc aaaagggtta
      481 gaagtccatc ctaagcatga agaagaacta aaaggtatgg cttcagctat ggaaaatgtc
      541 tgctggttct ttggccaagt actattcatc ggaggagcag gggcgttgct agtccaatca
      601 acactgaaag atctaggata tgacgtaaca ttgggacagc ttgctttagt ggaagttcca
      661 gtggctatca cggctttagt tgtggcaagt gtctacttct atcttaaaga aaagaaatta
      721 gcgaagaaat attatccagg aaaggggcaa taagatggac ttttttacaa atcctgatat
      781 tttattaggc gataaattac tggagatcct ttatatcgtg atgggactcg tattgattta
      841 cactggagta agaaatttaa cagataaaac caatcctcat cgttatgggt cagccttttt
      901 ctggacggtc ttaggggtag ttattgcagg aggaagatgg cttccggcaa tcgtgaacgg
      961 tggcttgatc tttgcgatga ccataccagc aattgctaag aaagtcagca aaggagaaag
     1021 ccggcttcca tcaaaagaat atatggaaaa aatgtcagac aaactaggaa tgaaaatttt
     1081 tattccagct ttgagtatcg gtgtctttgc gatcttgttt gctctattta ccaatttagg
     1141 tgctttagtc ggcgtgggcg tcggtgtttt tgcagccatg ctaatcttga tgttcttttc
     1201 tcgagataat cgaccaagta catttttaga tgatgcagca gatatgctgg gcacagtagg
     1261 gccgttaagc atgttgccaa tgcttttagc ttctctaggt gcggtattta caagcgctgg
     1321 agttgggaca gtgatttcca atgcagtagg tacgatcgtt ccagaaggaa atgtgaacat
     1381 cggcatcatc atttacgctc ttggcatggt gatatttacg gtaatcatgg ggaatgcatt
     1441 tgcggccatc accgtgatga cggttgggat cggtgcacca ttcgtattta gtcatggagc
     1501 gaatccagcg ttaataggaa tggttgcatt gacttgcggt ttttgtggga ccctgctgac
     1561 accgatggct gctaacttca atatcgtgcc tgtagcgatg ctggagatga aagacaaata
     1621 cggagtgatc aaaaaccagt tgtttattgc gttatttatg ctagttttcc aaattgctta
     1681 tatgattcta tttaaataat agaagagagg ggttgtgaca gaataattgt cgcaacctct
     1741 tctaacattg tagtggaaca ctattttttt ataagaatat attaagtttg atctgttatg
     1801 ttatttaaac tattgtttcc tttatttacg ctgttcaagg aaaatggtat gataaaagat
     1861 aaccatatgt taaatcaatc ggacggagga acaacagatg aaagtactag taacaggttt
     1921 tgatccattt ggtggggaca aagtgaatcc agcctatgaa gcagtaaaaa aaatgccaga
     1981 tgaaatcagc ggtgctgaaa tcatcaaagt ggaagtaccg accgtattcg aaaaaagcag
     2041 tcaagtagtc aaagaagcca tccaacagca ccaaccagat gtcgtgatct gtgtcggaca
     2101 agcaggtggg cggtcagcag tctcgttcga acgtgtcgca atcaatctag cagaagcaag
     2161 aatccctgat aatgaaggaa atcagccatt cgatacggct ttagaagaaa acggtccagc
     2221 cgcttacttt acctcattgc caatcaaagc aatgacaaaa aatgtacaag atcatggtct
     2281 accagcgtac atctcttata cagcaggaac atttgtctgc aatgatatca tgtatcgttt
     2341 gcttcatttg atcgaaacac aattcccggc aattcgaggt gggttcatcc acgtgccatt
     2401 ctcacccgat caagtgatcg atcgtccagt gggtacgcct tctatgtctt tagaagacat
     2461 tgcttcttct ttgacttatg cagttgaagc agcagtagaa aataaagaag atatttccgg
     2521 aaatgccgga acaactcact aatttagcaa gaaacactgg ctggttgcta acgttatgtt
     2581 agtagccagc tttttgtaat tagaaaggaa agaaacagcc gtggaagaat ggcaatccgt
     2641 ctttgaagaa tggtttccaa aagaaatcag caaaagctat cccatcaaaa tttctaaaca
     2701 atacacaagc agtcaacgtt gggagatcta tgcaaaatta acgaaaaaac agcgggaact
     2761 agtcgataag caccgccgat atctaatcag ctctcgtttt atggaagaac attatttagc
     2821 tgcaacagat tgggtgtttt ctgacttcaa gatcaatccg ttttttcgaa caaaacgaag
     2881 ccagcaaaaa ttgtattgtg agtgtggtag agaattaaaa gtgcaataca tcgttaaatc
     2941 gccaaaaacc ggaaagatcc taaaactagg catcaaccac tttgccgatc acctgcatgt
     3001 ctctccgaca gtggcagctt cgatccatca gggaatgaca aaagttgatc tggccttaga
     3061 cgaattatta tggcttaagc agaagaatat cgattttcct gaaggactat ggcaaaaata
     3121 ctgttttgtc ttgtaccaaa atcgtcggat gaaacagcca tatttaccgg atataaaatt
     3181 agctcagcgg ctcgcagaat ttcgccaagt agaaatgccg atttacatag cggattatca
     3241 agcattggaa aatgaaatca agaaaataag tgaacacatc aatgggcagt caaaaaagcg
     3301 acagataaaa aaagaactat ttgacgactt tgcagaagaa ttagtaaagg atgtagaaga
     3361 attcttgatc aactatcgcg catttttacg taaagattgg caatccatcg tatatgaaga
     3421 agtgccagta catcctaatg cttattttga aacatttatt tccgtgttga gaaagacaaa
     3481 acgtcaacga acaccagaag tcactgctca gatggaatat tttgcgaaaa accaacgatt
     3541 catccaacca aaaatctatc tatttatttg gaagcaatat tgtcgctatg gttttacaga
     3601 agggttcttc gacagcattc ctcggatagt cagaaatggt tttttaaaag tattgcgtaa
     3661 agaacgagaa gctatacaat ctgctgataa gaaagaccgt acagtctcaa aagaaaaatg
     3721 gcaactagta gtgaaagata tccaatctgg aaatgtgcaa gagacaatcg acaaatggaa
     3781 aggaaaacat tatcgtttta ctgaagctca gaaacaagcg ttggaatatt atcagaagtt
     3841 agaagaatca ctgcgtttca atgatgaggc aagaaagtat ctgaaggaat tactataaaa
     3901 aatagaggct gggacagaag cgtttaactc caagaaataa gaagaaattc acgaaaattg
     3961 cttttcaaat ttttgtgaat ttcagcttat ttccgaagga gttgcttctg attccgccgt
     4021 ttatacgtgt ttagagcgtg agacaaaagt gttctttact tttgtcccac gctcagtttt
     4081 tttatgcgtg ttgttgtgcg ttgtagtatt cttcttccat tttttgcata cgttcacgtt
     4141 ctttttggtt ataccaaggt gaaacggtaa atgcaaggac atcattcagc aagtaaacag
     4201 agctgttcac gaacatcgcc aaacttgcag atccatgcgt aaatgaaata taccaaagga
     4261 ccatttgagc aagccctgaa gctaaccacc agtaatattg gttgttgtaa cgcaagaaac
     4321 tgatgatacc agcagtcaag ctaatagaaa aagcaatggc atctacccac ggccgaggat
     4381 catctgtata acgaccaatc aggaatcctg aaacaagata gacaaggaaa gtactgatga
     4441 ttgcgataaa ccagcttttt ccagtgaatt ttcggatttt tgaagccata ttatgattcc
     4501 attctttact taacaatacg gggatatcca atgtgatcat ataagcaatc tgttcaccga
     4561 tacttaaata gttctttgct gaaaacccaa cgataataaa acaaatcgca gacaaaatac
     4621 ctaaaatacc gttgactgat tttgccgcat tgatggataa aatacacaat acaccaagcg
     4681 tggtaccgat aaatgtaata acggacagcc aagtgatggg actattgaca agagtcatga
     4741 cctggcagcc aagactgaaa aagaaaagat aataattttg ttgcggccag ccttttagct
     4801 gatgagctaa aaactgaaaa tatgacttca tgtatgagga ctccttaaga cactaaatgt
     4861 agtgtgttct gtatttacta acacaatcta ttgtatgctt ttcttgaaaa aaaactagtc
     4921 tttcaattat aaataatttg tgttatgata attttctgtt tgttttcttg aaaatataat
     4981 ttcttacttt catttattat attgggaaca ggaagagtgt cttggaggac ctactttctg
     5041 ttttttttgc cttttttctt ataaaaagga aaaacagcta tttttttcaa tctgacacat
     5101 ttacaatgga actggagatt aatatagatc cgagcttaaa cgaagaatag gagtgctcat
     5161 atgaacctta caacgatcca tcatatagcc atcattgttt cagattatca gaaatcacgt
     5221 gatttttatg ttaataaatt aggatttgaa gtaatcagag aaaattaccg caaagaacga
     5281 gatgactata aattagatct gaaactagga agtgctgaat tggagatttt tggaattgct
     5341 aatgcgccac aacgaccaaa ctatccagaa gcttgtggct tgcgtcatct tgctttcaaa
     5401 gtagacgata ttgaagaaac aattgcagaa ttgcagtcct tagggatcga atcagaaccg
     5461 attagagaag atacctttac aggaaaaaaa atgacgtttt tctttgatcc agacggattg
     5521 ccattagaac tgcacgaata aagataacaa aattgtcact tttttttcgt ttcattcgat
     5581 ttttcattcg ttcatttctt gatattcttt aaaaaaaaga aacaggagaa tgaagaatga
     5641 aaaaaatgat gtttggtgtt gctgccctca tcgtaatagg gattaccggt atgacgggac
     5701 tgctcaatgc cgcttttcac atggatgata ttagtgacca ggaatatcgg atctcattga
     5761 aaaaagcagc agcaatcgta aaaaacgaaa caaaagaaca taacatatct gctattcatt
     5821 ttgcagttga aaaagatttg gagaatccta cgaatgcaac atatgaatac cagttttttt
     5881 gtgaaggaca agtcatagca gtcaatccat ccactgggaa aacaaccgta cgaaatgaca
     5941 cggaaaaggc cgcagaacat ctttttgata tagaaaaaac ggaagagttc aaacaaccgc
     6001 aagcagcgat gaaggaagca gtcagctact acgggaaaaa agcaaaagtg aaagagtgga
     6061 cacttctagt caaagatgaa cgtccttatt ataaagtccg attaatagat ggagaacagg
     6121 accgtcaggt atggatatct gcatagaaga aggatattgc ttctcttttt tacaatataa
     6181 gagtaagcta agagagtaaa gatgaaagga agttgagata acatggaaaa aatgaaatgc
     6241 cctaattgcg gaaaaaaatt tgcatacgaa gaagtcaata atgtagtcga acataatgat
     6301 aaagaaatgc cagtcgtctg cccttattgc cgaactgaag cggcaagaat cgttactcat
     6361 ggttatttca ttactgaaaa gattgaagat tttttaaaat aacgattttg cgatttcttt
     6421 ttctgcatgt tagaatggag aaaaggagag gaaactatga ttcgttttgc gagaaaagaa
     6481 gatgctccag aaatcgcccc attgatctta gtgattctaa aagatatgga actgccattt
     6541 ttgaagcaga caggggaaca aaagacttta gagattttag aagaagcaat cactgatcca
     6601 aattatcgtt acagttattc acgaggtatc gtagaagaaa tagacggtaa agttgcagga
     6661 gttgcttacg gttatacaga tgaagaagaa tcaatcatcg atcagccact ggtacctatc
     6721 ttagagaaat atcagctaga tgcgtctaca cgtttgttca ttgataaaga aacgtttccc
     6781 aacgaatggt atttggactc tatcagtgta tcagaagatt ttagaggaca aggcatcggt
     6841 tcgcgattat tagaagcatt acctaaattg gcgaagaaag ctaatcgctc cgtcatcggt
     6901 ctaagtgtgg atgaaaaaaa tccaaaggct aaaaaattat acgaacgcca tgacttcaaa
     6961 gttgtcggtc agcggatgat cagtggtcat ctttatgatc atatgcaaaa gaatatctaa
     7021 aaagatgtac ctgaaatgcg tatttccgca tttcaggtac atctttttaa cttaagttaa
     7081 gtcggtaaga aacaggctta tcgcgacttt tgtctttacg gtagagccag taatctaaaa
     7141 ccgcagcaat acaaatacaa actggagcat cttcatcatt tgagacatcc aatacatagt
     7201 aatctcctga aaaaagtgtt gctttatcca ttttcatgat ttgctgattg aaatgatgga
     7261 tttgatattg atgattatag atatcacctt ggactgtcca gtgaagtcta cgaatataat
     7321 aaaagtcacc gggccaattg aatattttct gcatggttcc caccttttcg aactctttat
     7381 acagctcaaa acgcgtaccg aatgtcaggc ttatctgctt gatgtgagca acgagatcgc
     7441 cattcatggc ataaagagac aatgcatcgc cctttgttcc ccagcgacca accatcaaaa
     7501 acagggaatg tcctttttcg tttttgacaa ttgtccgagt tgcacgtcca agctgctttt
     7561 cctgaatgaa aaattctgac atctatatcc ctcttttcta gataaaacta caagtcttct
     7621 gcaatcgcgc gcaatatggc ttttcctacg ccagattcaa ggtttgtgtc cgttgtatat
     7681 ttggcataat cttttacttc gatttctgca tttcccattg cgaaactgac tcctgctact
     7741 tgcagcatgc tgacatcatt gaagttatcg ccaatcgtca tgacttcttc taaagaaatc
     7801 ccacgctcgt gagcgacatg ggcaacagca attccttttt gagcgttttt atggttcact
     7861 tcgatattgt tttgaccaga ggaagtaacg gctagatcgc ctaaaagaga aatctgcttt
     7921 ccaactggac ctaatacagc tggcccttct gtatgaaaac aaataatttt taaaacttcc
     7981 agattctttt cttccaaaat agcacgaata ctgtccacgt atgtgatgtg caataactcc
     8041 agcttagctg aggccatggc aatcgccatt ttatacgtaa ggtgaggtaa atgggtagcc
     8101 accatggaag caaaactttc aattcgtttc gcttgatttt cagaataaag accatcgttg
     8161 gttgctactt catagtaaat accgttagca tctagaatat ctaaaacaga gattgcttga
     8221 ttatttggaa tcgacacggt aaacaatgaa tttccttctt tatcaaatac ctgtgccccg
     8281 ttcaaagtga tcatcgcaca atcgattcct acctcatcta aagctgcgcg agcttcttga
     8341 tagtttcgac cagtagcgac catgaactca acaccggcat catttgcaaa acgtatcgcc
     8401 tcggcatttt caggagaaac actcatatgg gcatctaata atgtgccgtc catatctgat
     8461 gcaattaatt tgatcataat gattatcgtt cctttctgct tcttgttcct agtttaccat
     8521 attaatcaag tgctgagggt gaagattgtc ccttgtgatt gaagaaaatg tgagttattc
     8581 tttttattag gaggtggagt ttttgaaaga aatcattcat aacagttggc aagaagtttt
     8641 gtcctcagaa ttttcaaaag attattatct gcatctaaga gaatttttaa aaaaagaata
     8701 tgcgtcccaa aagatccatc cagatatgta tcatatctat gaagcattgg aattgactcc
     8761 ttatgaagaa gttaaagtgg tgattttagg acaagatcct tatcatggag aaaatcaagc
     8821 tcatggctta tctttttctg tgcaacctgg cgtaaagatc ccgccttcgt taagaaatat
     8881 ctataaagag ctttatgatg atcttggaat cgcacctgtt caacatggaa atctagtgtc
     8941 atgggctaaa cagggagtcc ttttactcaa caccgttctt accgttcggg aagggcaagc
     9001 gtattctcat cgcgggaaag gttgggaaag attaacagac acgatcattg aaaaattaaa
     9061 tgaacgggaa aaaccaatcg tgtttatctt gtggggaaaa ccggcacaag aaaaaatcaa
     9121 aatgatcgat aagagtcgcc atatcatcat aacatctcca catcccagtc cgttatctgc
     9181 aagtcgtggc ttttttggtt ccaaaccctt ctcgaaaacg aatgatgcgc tccttgcgtt
     9241 aggagaagaa ccgattgatt ggcaactgcc tgaaacagtg taataaaaca gcgctttcct
     9301 gttatagtgc agttcgcttt gtatgtgaaa aacttaattt ttccagcatt tttgactgat
     9361 acagataaaa taaactgtta tttttttctg gaatagtgta tgataataca tataaaaacg
     9421 tctggaggtc atcttgtgga attgtttgat agtttaagat ttaaaattgt tcgccgtggg
     9481 atcaaaatcg ttttcccgga agctactgat gctcgtattt taggcgcagc tgcccggttg
     9541 aaagcagaag aacttgtgga acccatttta gtagggaacg aagaagaaat aagaaatgca
     9601 gcgcatgcca gaggaatcaa aacatctagc tttacgatca tcagccctga taattacgat
     9661 aagtgggatg aaatggtgga tacttttgtt gaacgtagaa atggaaaggc atccaaagaa
     9721 caagcagaaa agattttaaa agatgtgaat tactttggta cgatgcttac gtatatgggg
     9781 attgcagacg gaatggtctc tggagcgatc cattcgactg gcgacactgt tcgcccagca
     9841 cttcagatca tcaaaacaaa gcctggcatc agtcggacaa gcggagcgat gatcatggta
     9901 cgtggaagag atcaagaaaa atatgtcttt tcggattgtg cgatcaacgt gaatccaact
     9961 gcgcaagaat tggctgaaat cgccgtagat tctgcaaaaa cagcagaatt gttcgatatc
    10021 gaaccaaaag tagcaatgat gagcttctcg acaaaaggct cagcgaaatc gccagaagta
    10081 gacaaagtag ttgaagcaac taaaatcgct aaaaaactag cgccacaata catgatcgac
    10141 ggcgaattgc aatttgatgc ttcttatgtt ccttcagttg cacaactgaa agcgccagat
    10201 tcagaagtag ctggtcaagc aacagtcttt gtatttccag aattgcagtc aggaaatatc
    10261 ggctataaaa ttgctcagcg tttcggtaac tttgaagcaa tcggaccaat tctacaaggc
    10321 ttgaacaaac cggtttctga tttgtctcgt ggagcaaatg aagaagatgt atataaatta
    10381 tcgattatca cagcagcaca aacattgatt gattaacaaa tacacgcgaa ctagccaaaa
    10441 acagcagctg ctgttcttga ctagttcatt ttttctttaa aaaaacttca ttacgcgata
    10501 tactggcaaa aagaaagaag agcgggagaa gatgaaaaat gatcgaacta tcaggattaa
    10561 caatgacaga acaattcgca gaggcaattg gtcattccgc aatgccaggt gacaatctga
    10621 tattgactgg tgatctaggt gcgggtaaaa caacgttgac gaaaggaatt gcccaaggat
    10681 tagggataac gcaaatgatc aaaagtccga catacacgat tattagagaa tatagtcaag
    10741 gtcggattcc gttgtatcat atggatattt atcgggtagc agctagcgga gcagatttag
    10801 ggttagatga atattttgaa ggtgatgggc tttccgttgt tgaatgggga aatctattag
    10861 aagaagcact gccggaggat tatttggaac tgattttaga aaaaagtgat acagatttag
    10921 aaaagcggta tgtgaaacta atctcatacg gggagcaaag tgaaaccttc aaacagcgaa
    10981 ttctagaaaa atgggctgaa aatcatgagt gaagaagttg ggatcatctt gcgggaagca
    11041 gtgccaggag atgcaaaaga cattcttttg atgatgggac aagttaataa ggagacagaa
    11101 tttttagttt tggatgaagc agaactcctg ttaccaccag aaaccttaga agaagagttg
    11161 gattatatat acgaaagcaa taataattta ttgctgcttg caatctatga agggacgatt
    11221 attggaacag catctgtcaa ggcagacagt caatttcgtt tgtctcacgt gggagaagtc
    11281 ggtatcagta ttctgcagga atattgggga atgggactag gcactcttat gctagaagaa
    11341 atcatcagtt gggcgaagga aatgggtgta ctttttcgat tggagcttga cgttcaagtt
    11401 cgtaatgaaa gagccgtaca cctttaccga aaaatgggct tccaaatcga agcggtcatg
    11461 cctagagggg caagaacaga tctaggtgaa tttttagatg tatataaaat gagttatttg
    11521 attgaataga agaaatagga aaagagtgag aaactgtcat ccttttgaga aggcagaaaa
    11581 acacacgaag ggactgtgac aaaagtcgtg tcgcagtcca ttttttttga ataaacggtg
    11641 ttcaatcagc tgaccttcgg tattagctca aaaaacggca aaatatgagg aactattttg
    11701 ccgtttgttt ctctaatact caggtcaaaa cgctgatttc acaacctctt ggtaaacggt
    11761 gtccaaaagc ttgcatctgc ggtaataagt ccaaccaaat gaaaaatatg agaagctatt
    11821 tttgtttggc tgttcttatt acttgatgca gaacagcttt ttcacaacct ctccaataaa
    11881 cggtgttctc ctgccagctc ctcggtcacc agtcacaata tttaataaac atgagaagct
    11941 attttttgaa attgctcctt gtgactcgga gtgaaacggc aggctcacga cctctttgtg
    12001 tgtttcataa gtattctttt actttatagt cacaagtttt ttcaatggat ctgtcccgaa
    12061 ttttttttct tgataaagca ggatttccgc acatgcacga ctatcttcca atgcatcatg
    12121 gtgatgatct aattggatgt tcaaattttc gcacacagtg ttcaatcgat gatttggaaa
    12181 ttcaggaaac aggcgacggc tacttttcac tgtacataaa gaaagataat taggctgttc
    12241 aagatgataa taatctaagc atcccgctaa gacctttgta tcaaaagcag cattatgtgc
    12301 aacgactaaa cgattttttt gaaaatatgg ttggatagtt tgccagactt ctgggaattt
    12361 cggcgcattg cgtacgtctt cttgatggat tccgtgaatt tggacatttc gccagaaaaa
    12421 aggagtctca ggctgtatca atgtatagaa ttcatcaacg atctggctat tttttaccat
    12481 gactagcgca agagaacaag cactataagg ctgatgattg gcagtttcaa aatccatggc
    12541 aacaaaattc atttctgtaa aacctccttg ggaaattact gtaacatact tttgaaaaaa
    12601 gcaaataaat ttaacaaaaa acatagaatt ttttatgtaa tatttgttga gaaaagctag
    12661 tttttgaact cattccttgt gatttggagt tagatgggga tcatgacttc tcggtattaa
    12721 gtcaaattta tgcaaagttg tgaggaacca ttttcaaaaa aatttcttac gactcgaaag
    12781 taggcgactt tttcacaact tctccactaa acggtgttca atcagctgac cctcggtatt
    12841 agctcaaaaa atagcaaaat atgaggaact attttgccat ttgtttctct aatactcagg
    12901 tcaggacgct gatttcacaa cttctcaata aacggtgttc aaaagcttgc atctgcggta
    12961 ataagtccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt cttattactt
    13021 gatgcagtgc agctttttca caacctctta ataaacggtg ttcaaaagct tgcatctgcg
    13081 gtaataagcc caaccaaacg aaaaatatga ggaactattt ttgtttggct gttcttatta
    13141 cttgatgcag tgcagctttt tcacaacctc ttaataaacg gtgttcaaaa gcttgtatct
    13201 gcggtaataa gcccaaccaa acgaaaaata tgaggaacta tttttgtttg gctgttctta
    13261 ttacttgatg caggacagct ttttcacaat ctctcgataa acagtgttca aaagccagtt
    13321 tctcggtatt atgccaaatt tatgcaaaat gtggaaagcc attttcacaa aatttttctt
    13381 aatactcgaa actaaacgac tttttcacaa cctctttatt tcaaatctaa ttctactgga
    13441 caatgatcac tgcccataat gtccgtatgg atctttgcat cagctagtcc tggctctaaa
    13501 tctttgctcg tcaaaaagta atcgatccgc caaccagcat tgtttttcct tgaattgaat
    13561 cggtagttcc accaagagta gacaccttcc tgggttggat aaaaataacg gaatgtatcg
    13621 ataaacccgt tatccaacag tcgagaaaga gccgctcttt cttctggagt gaatccagca
    13681 tttttttgat tcgttttcca gtttttcaaa tcaatctttt gatgagcgac atttagatcc
    13741 ccacacacga tgactggttt ttgttttttt agattttcca aataattata aaaggcttct
    13801 tcccattcta atcggtaatc taaacgtttt aattcgcttt gagaatttgg cgtgtaacag
    13861 gttacaagaa agaaatcgga atattccaaa gtaatcagac ggccttctgt atcatggata
    13921 tcgataccca ttccgtaagt ggcattcaat gctggttttt tagcaaaaat cgctgtgccg
    13981 gaatagcctt ttcgttccgc gtaattccaa tattgataat aacctggaag atctaaatca
    14041 atttgtcctg cttggagttt cgtttcctgc agacagaaaa aatcggcgtc taactcatga
    14101 aaaacttcta aaaaattttt atttacgatg gcacgaagcc cgtttacgtt ccaagagata
    14161 aatttcaata aaagcacact ccttcgaatt aatttcctta cctttgcgat actattgtac
    14221 ctcattttag aaaaaaatgg tgtaagatga aagtaactaa atgatccagg aggaaagtag
    14281 atgaagattg gtgtaatcgg tgtagggaat atcgcagaaa aagcatattt accaacttat
    14341 gcgaaaaaac aaggaacact tgatttttat tttgcaacaa gaaataaaga aacaaaagaa
    14401 agaattcaaa aagagtacgg gttccaacat ctatatgaaa cgatcgatga gcttttagaa
    14461 gaaaaaatcg aagcatgtat gatccacgca gcaacaaaag tacattatga attagccaaa
    14521 aaatgtctag aaaatggtat ccatgtttat attgacaaac cactgagtgt ccatctaaat
    14581 gaagttcgtg aattgcaaga attagctgat aagaaccaaa taattttgat gatcggtttc
    14641 aaccggcgct ttgctcctat ggtagaagaa ttgaaacgta tcccagataa gcggttgatc
    14701 cacctgcaaa aaaatcgaat agcggcaaaa gaaacgacag agttcgtcct ttatgatttg
    14761 tttttacatc ttattgatac agctgtgtat ttattagatg aacccatttt gagaacaaaa
    14821 taccagataa gagaaacaaa ggaatttttg gaatatgcca ttttacaatt agaaacacaa
    14881 aatcaaacgg ctattttaac gatggattta gcaagtggcg ctaatataga aaaatatcaa
    14941 gtaacaagta aaaatggtac ttatgaagta aacgatctga cccagcttgt cattcaagat
    15001 gctgatggaa aaaaagtgaa agaatttggc gactggacaa acacgctagt caaacgcggg
    15061 tttgagccaa tggtagaggc cttcttttct cagatccgtt taggtaaatc ggaaacagaa
    15121 ttgcttaaac aaaaagaagt tgtaagaagt catgaattat gtgaggaagt gcttaaagat
    15181 cactaccgtc atcaactttg aacaagactg gcaagattgt attaagcgtg atagaataat
    15241 gaggacgttt gagaaaagaa aggaaaatcc tcagtgaata aagaagaaat agttgaaagc
    15301 ttagccacga ttactatatt gtttgatgaa ccattgatga actatacgtt tacgaaaaca
    15361 ggtggaccag ctgatgtcct tgcattcccg aaaaaacaag aagaagtaaa gcaaatcatc
    15421 gattattgcc gaatccatga tataccttgg atggtattag gaaatgcaag caatctgatc
    15481 gttcaagatg gcgggattcg cggtgtagtc atcatgctga ctgaaatgaa acagattcat
    15541 gtcaaaggaa cgatggtcat tgcggaagca ggtgcctcac tgattgatac gacttatgca
    15601 gctttggcag aatcattgac tggatttgaa tttgcttgcg gcatcccggg aagtgtcggt
    15661 ggagcggttt atatgaatgc aggtgcgtat ggcggagaga tcaaagatgt ttttgctgaa
    15721 gtggatcttt tgcttgaaga tggaacattg aaaactttga caaaagacga aatggtattt
    15781 tcttatcgtc acagtaaagt ccaagaatta cgtgctatcg tattggaagc tcgattcagt
    15841 ttgcaaacag gggattatga agcaatcaaa gcaagaatgg atgagttgac cgaattgcgt
    15901 caatcaaaac agccattaga atacccttca tgcggaagtg tctttaagcg cccagtagga
    15961 cactatacag gtcagctgat ccaacaagca ggattgcaag gcctgaaatg gggcggtgca
    16021 caagtctctg aaaaacatgc tggattcatc gtcaatatcg atcatgcgac agcgacagat
    16081 tatattgaat tgattgcgca catccaagaa gtaatcaaag aaaaatttga tgtgtcttta
    16141 gaaacagaag tgcggatcat tggagaaaaa caagcaatat cagaaacaag cagaagttta
    16201 gccaattaat aagtaatgga acttaaaata agtaaaagag caggacggtg gccgtcctgc
    16261 tcttttttgt ggttgtttac gcaaatggcg caaatcttgt acattttaac acaataaaac
    16321 aaaaaatata gaatatttcc tcataaattt ttccattttt tgcaatattc tcaaaaaaaa
    16381 gataaaaaat gagaataact gatgaaaaaa caagcataat ccatcataaa atcagtatag
    16441 gtgaaaagat gaactttcat ctgcatatca agtaattggt atgaggcatt ttttgaggtg
    16501 taggatatga agttggatta ccttatagaa gaggaagata gagaaaaact gctgctagct
    16561 agatatttag aatttgagaa aaagaattat gtagtgctgc ctaagttata tgaaacatta
    16621 ggtttgtcta aatttaaaac aagaaattat atcatagaat taaataaaga atttactgaa
    16681 ttcaaaacaa agccagagat ccaaatttca gaagaagcag agatccatgt cgaacgtctg
    16741 aatttgacgg atatcaaaaa tttaagaaga atctactttc aacgatcaaa aattgctcga
    16801 ctgttaacgg agatcgtgga ttcaaaagta acgatcgaaa aatttgcaga gaatcatttt
    16861 ttaagcatat caagagccta tgtaaaaaga agagaattag tctcttttct tgaacagcat
    16921 caaataaagt taaagaaaaa catattagtt ggggaagaaa taaacatacg gaacgtatta
    16981 tttggggtat atttcggggt ctttaatggt ttttctatgc catttagaga aagcgtcatg
    17041 aatgaagcaa agagtatcgt gcaatatttt gaatatctgt tcaatttaaa actgtctgca
    17101 acaaaacgaa tcaaattcaa tttgctaacc actattagtt tgttgagggc ctatgggaat
    17161 caaccaatcg gaaaacgttt cttttcagat gaatacttta agagctcaga aggaatgaag
    17221 atcatcaagg atttatcaca tatttctttt cttcatatcc ctaaagagca gatcattgat
    17281 gagatttctt atatcatgtt gtttttgatc acggaagaag aatttgaatt agatggcgca
    17341 tttggacttg cggaagctca ttttcatgat atccacatga ttagtcaaaa aatgtcagtg
    17401 gaattgattg atcaattacc attttcgatt gaaatctctg aagaagaaag actatatata
    17461 cgaaaagaac ttcgtatggg tatgatgcat gtcaatcgaa aaaattggat attcgacttt
    17521 gagatgacta gctttacgtc taaaaagcag ctacagtttt tcttcgagac gtatccaata
    17581 ttcagtacag caatccaaaa ggtcatacaa gagaataaac gctatttctt gggaaaagat
    17641 gaacgtttca tgattcgctt attttatgat tatctctttt tattggtcag tatttgtccg
    17701 gtatcatatt ttgaagcacc gattcatgtc tgtattgatt tttcgcaagg atctgcttat
    17761 accgattaca ttatttctca agtgaaagga tttaaaaatt acaatattgt gattgagagt
    17821 cgaatcactt ctcgtacgaa tatttattta tctgattgtt tacttgaaaa atttaataaa
    17881 gaacagataa tttggaaaaa tccaccgact ccagatgact gggaatattt tggaaatacc
    17941 atcatcagag aaaaaaatag acatttaact gaaaaaatca gctagtattt atggatggca
    18001 gctttgtctc acatatttat tagctgattt atgttttttt caaagaaaag gtaactagat
    18061 ataactagat ataactagat atattactta acaaaattaa aaagtacata agttattatt
    18121 ccgattttct tgaaacaata aaaaatcaaa aaaaatactc tcctaatagt ccatcttctt
    18181 ttgattttat ttatataatg aaatcatctt atgaaaatta ctacttcatg tttagacaag
    18241 agtagggagg actaagaata aatgtgttca aaaagacgtt caaaacaact aaataaacac
    18301 gcttgtcgtt tagataagga acggtcgtgt tctgaaaaac actacgttaa gtttatgatg
    18361 cgcacgatgc tgatcagttt atctgtaggt gtctatatgc atgtacccgt tcaggcaacg
    18421 acacaaacca tcaaggtcgt acgtacctct gtttctgaga ccactgattc ccctatcact
    18481 attactcata ctttcactga tgcaaatgga aaacagctac ttcctgcatt gacttcctat
    18541 aataaagtca gttatcctcc aacgataaac cactactggt taattcctaa caaaacatta
    18601 tggacatcgg aaaccggaag cagtgtgccg ctttctatct tttccgatat gactgatcct
    18661 acaaaaggtg atcagttaaa acagatcact gatattttga atcatgcttt gacagatata
    18721 aaggacggtg gaagcgcaca agtttcttat gtatatgaaa aagattattc acagtttgaa
    18781 agaaagaaaa tcgatgttct ggcagggaca aaagtttcga tatctgatct ggtagaaagg
    18841 ctgaagaacg cagatggaac acaaggagat atgtccaaag taatttcaaa caatgggaat
    18901 atcgtggata cgactaatac aggaacatct actatttcct tgtcctattt tgatccaatt
    18961 gctttaaaaa caatgactgc atctgctgtg atccatgttc gtgctgatca aacagctatc
    19021 aaaggaaaag atcttcactt aagaacagga gagagttacg cccctaaaga tcttgttgaa
    19081 tatgtaacaa aaagcgatgg aacttcagga aataaagaag aaatcaaaat gaacgggaca
    19141 gtagatacta caactccggg agaatatccc gttgtattaa cttatataga ctcgaaaaca
    19201 tcaaatgtgg cagaaacaac agctgtagtt tatgtagaag catctgctgc ttcatcgact
    19261 gtcatcactc attttataga tagtcaaaca aataaagaac ttcaagcaga aacacatatg
    19321 agtagtgtaa atcgtattcc tgatttgcgc ctacctgctg aattttctga tcgagtgcca
    19381 aatatagatt tatcgactta tgaacaaaat gggaaagtca cttctatttc tgattggatg
    19441 caagcaaacg gattagacag aaaaagtcac tggtcggaga tccttgaagt acttcactca
    19501 gaactgatga ataataggaa gcaatcagga ggggaaatta cctttactta tgtttatata
    19561 ccagacaatc gttcattaga aggtcatgat cttattgttc atccaaacga aaaaataaac
    19621 aaagaagagt tgatccaatc agcaacaaat tccttaggta aacccgtacc gttaaaggat
    19681 gtatcggtta caatcaatga agtaccattg gaggatggtt atttgaccaa acaaacaggt
    19741 gattttacga ttatgtatag ttatcatgac ccgtattcac tccaagaact ccatgctcag
    19801 tcactattac atgttttacc attaaacaaa cagatagatc aaaaagaaca aaaaagacaa
    19861 gaagaaagct tgtcagaaaa aaaacaacaa atggctcatg cagtttctcc aacagggaaa
    19921 gtaaaacaaa caagaagcct agagttagca agaatatcta gaaaaatctc agaatctaat
    19981 aaaacgataa ggcaagaagc tcatttggcc ccacaagttt ctggattgga aaatgaaatt
    20041 ttagagccta aaataaaaca acaatcagaa aaagaatttg ttgctaaaca gtcggaagat
    20101 cagaaggaat cagatgacca gaaggaaacg aagaaaaaac gcttgtcaac tattgattcc
    20161 accgatgagc atctgcagcc gcaagtacca cttcctagtc cgggaacacc cgcacctggt
    20221 ggaggtattc cttcttctgc tttaggagat ttcatgcgag gtatggcagg aacatgggta
    20281 tacgctaatc gaaatgaatt tgacgcagat gactggttct gatttatatc tctatctgtt
    20341 atagtcaaat aagtagctga gtcatatgtt atttggcata atggagacga ggagggatag
    20401 aaaaatgtta gataaaacgt taccttacgc agaaatctgg atgacaagac cacttcaaca
    20461 aaatgttcca atcacgcctt tagcggaagg ttttgaattt cgattttatc aaaaagaaga
    20521 tgaatctgct tgggcggaga ttgagacttc cgttggggaa tttcaaacga cagatgaagc
    20581 acttgagtat tttcaacaaa actttgctcc ttatcctgaa gaacttgaaa gaaggatgtt
    20641 ttttgtagag acaaaaaaag gagaaaaagt agcaacctgt accgcttggc gaaaaaaatc
    20701 aagagagcaa gtgtatccgg tttttcattg gttagcagtg aaatcagatt accaaggaaa
    20761 agggctcgca aaagcattga ctgccagagt cttgcaagaa tttcctaccc tccatacgga
    20821 cggaccaatc tatcttcaca cacagacatg gagccatcag gcgattgctc tttataaaaa
    20881 aatgggtttt acttttattg ctgaaaatct agacggcaca cctaatccag attatgaaaa
    20941 agttatgcag gttttaagta aattagataa ataacgaaat ggtgaaataa gtcctcagta
    21001 ttgggataaa aaacgaaata gtcattcctc atatcttact tttttgagtt aatactatcg
    21061 gataactaat ggcatgcc
//
LOCUS       DAZBTF010000051        20673 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000051 DAZBTF010000000
VERSION     DAZBTF010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.051"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..1581)
                     /gene="ahpF"
                     /locus_tag="ACHGNS_001908"
     CDS             complement(52..1581)
                     /gene="ahpF"
                     /locus_tag="ACHGNS_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFN6937841.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1591..2154)
                     /gene="ahpC"
                     /locus_tag="ACHGNS_001909"
     CDS             complement(1591..2154)
                     /gene="ahpC"
                     /locus_tag="ACHGNS_001909"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFN6937842.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2298..2960)
                     /locus_tag="ACHGNS_001910"
     CDS             complement(2298..2960)
                     /locus_tag="ACHGNS_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN6937843.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            3144..4790
                     /locus_tag="ACHGNS_001911"
     CDS             3144..4790
                     /locus_tag="ACHGNS_001911"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFN6937844.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4781..5863
                     /gene="uxuA"
                     /locus_tag="ACHGNS_001912"
     CDS             4781..5863
                     /gene="uxuA"
                     /locus_tag="ACHGNS_001912"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFN6937845.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(5971..7524)
                     /locus_tag="ACHGNS_001913"
                     /pseudo
     CDS             complement(5971..7524)
                     /locus_tag="ACHGNS_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            7672..8813
                     /locus_tag="ACHGNS_001914"
                     /pseudo
     CDS             7672..8813
                     /locus_tag="ACHGNS_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(8939..10018)
                     /locus_tag="ACHGNS_001915"
     CDS             complement(8939..10018)
                     /locus_tag="ACHGNS_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFN6937846.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(10121..10561)
                     /locus_tag="ACHGNS_001916"
     CDS             complement(10121..10561)
                     /locus_tag="ACHGNS_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFN6937847.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(10618..11028)
                     /locus_tag="ACHGNS_001917"
     CDS             complement(10618..11028)
                     /locus_tag="ACHGNS_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFN6937848.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(11208..11642)
                     /locus_tag="ACHGNS_001918"
     CDS             complement(11208..11642)
                     /locus_tag="ACHGNS_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN6937849.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(11742..12590)
                     /locus_tag="ACHGNS_001919"
     CDS             complement(11742..12590)
                     /locus_tag="ACHGNS_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN6937850.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            12979..14745
                     /locus_tag="ACHGNS_001920"
     CDS             12979..14745
                     /locus_tag="ACHGNS_001920"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFN6937851.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            14847..16106
                     /locus_tag="ACHGNS_001921"
     CDS             14847..16106
                     /locus_tag="ACHGNS_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFN6937852.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            16253..17554
                     /locus_tag="ACHGNS_001922"
     CDS             16253..17554
                     /locus_tag="ACHGNS_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6937853.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            17557..18408
                     /locus_tag="ACHGNS_001923"
     CDS             17557..18408
                     /locus_tag="ACHGNS_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFN6937854.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            18421..19236
                     /locus_tag="ACHGNS_001924"
     CDS             18421..19236
                     /locus_tag="ACHGNS_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFN6937855.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            19387..20553
                     /locus_tag="ACHGNS_001925"
     CDS             19387..20553
                     /locus_tag="ACHGNS_001925"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN6937856.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
BASE COUNT         6847 a         3738 c         3836 g         6252 t
ORIGIN      
        1 tcttgtcacc atcctttttt ccgctctctg actttttaca atcttcatag ctcaagtgcg
       61 catcagataa tcgaatgctc ctaaagctgc agtagctcct gaacccattg aaacaatgat
      121 ttgtttgtat ggactatctg tacaatcacc agctgcgaaa acgccgggga tattcgttgc
      181 gccatttttc tcggtcacta tttcatttct tggattcatt tcgatcgttc catctagcca
      241 atccgtatta gggagtaagc cgactagaat gaatactcct gaaacagcta gtgtatgcat
      301 ctccatcgta cggcgatcga tataggagag agattctacc tgattcgtac cgctgatttc
      361 tttcgtcgct gcatgtttga taacagtgac attcgcaaga gagactagtt tatcctgtaa
      421 gatttgatct gcttttaatt ctggtaagaa ttctagaaca tatacatgtt ttgctaaccc
      481 agccaaatcg attgctgctt caacaccaga attaccacca ccgataacag caatctcttt
      541 tcctttaaat aaagggccgt cacagtgtgg gcaataagca attcctttat ttttaaactc
      601 tttttcgcct ggtacattga tagaacgcca gtgcgcacct gtcgcgatga tggcggtttt
      661 tgctttaagt gatataccgt tagctaagcc aatattaata aaatgttgct ctttatttaa
      721 ggaaacggcc tgttgattca tcataatgtc gatagggtaa ctacgcacat gactttctac
      781 ttgctgcatc aattttggac cttctgtgta agaggtacca atcatatttt caatccctag
      841 tgtttcaacg acttgtccgc cgaaagaatc agcgacaagt cctgtacgga ttcctttacg
      901 tgctgcatag atagctgcac tactagctgc tggtccaccg ccaatcacta atacatcata
      961 aggttctttt tcaaacaact cttcttcaat caaagggcca gtgatttttt caacaatttg
     1021 ttcgatcgtc atgcgtccgc tactaaattc ttctccattt aaaaacacag ttggcactgc
     1081 catgattttt ttatcatcga tttctttttg gaacatccca ccttcaatca tcgtatgagt
     1141 aatatttgga tttaatacag ctaatatatt caatgcctgt acaacgtcgg ggcaattatg
     1201 acaagtcaag ctaacataac tgtcaaaatg caatgtttgt gttatttttt tgattttaag
     1261 actcaaagat tcactgattt tcggtgcccg tccactaatt tgtaacatag ccagtaaaaa
     1321 agaggaaaat tcatgaccga gggggatacc agcaaaaacg attccacttt ctttgccaac
     1381 tggattgata gaaaaactag gtgtgcgcat cgaatctctt ttttcccaag agattttagg
     1441 agacatttct gctatttcct tcgtaaattc agctagtttt tgactatttt cactattatc
     1501 caaactaaca gaaaaaacaa taggagattc aatcagtgct aaatattgtt cgagattttg
     1561 tttagtttct ttgtctaaca tatccaccac ctaaatcttt ccaacaagat caaaacttgg
     1621 tttcaatgtt tcgccacttt ctttccattt tgccggacaa acttcacctg ggtgggtacg
     1681 tacatattga gcagcgcgaa tcttatcaat cagtgcacta gcgtcacgac cgattccatc
     1741 tgcattgatt tccatagctt gaacgattcc gtctggatca ataataaatg ttcctcgctg
     1801 agctaatcct tgctcttcat ccaaaacatc aaaaatacga gaaatctgat gagacggatc
     1861 accgatcatc acgtattcga ttttaccgat agcatctgat gtatcatgcc atgctttgtg
     1921 agtaaagtgg gtatcggtag agcaagaaaa gacttcaaca cctaaagatt tcaatgtact
     1981 gtattgttct tgtaagtctt ctaattcagt aggacaaaca aaagtaaaat cagctggata
     2041 gaagcaaaga atactccagt tccccataag atctttttca ctgacttcta aaaattctcc
     2101 ttgatgataa gcatgtgcat taaattcttc gatttttttc ccaatcaatg acatagtaaa
     2161 acctccaata ataattattt atttataatt attgtaattt agttggagtt gcaaagtcaa
     2221 tggatatttt gttatttaat aaagattact caaaaacgaa ggttaattat aaaaatagaa
     2281 gaggtcgcaa gaaaatgcta ctgctggaaa tactcgggat aacgttgagt aacagtttcc
     2341 tgttctttca gcatcatgtg taagtgcaat cccgtttgaa atcgagcttc ttctggtttt
     2401 ttatcaacta aagccttgag taaatcataa tgttgttcca tgatcgtttc ccatggaagc
     2461 ccctgaatgg tcactcgtaa ccagcggaaa cgttctaaat gggtattatg gtcatctaac
     2521 cagttccaaa cttcttttct tcctgcaatt tcgaaacaag cttcatgaaa taaattatct
     2581 gcacggaaaa aagcgcgttc gtcatttatt ttgactgctt tttcttgttc ttctaaaatc
     2641 gtttctagca ctattcgtgt ttgtgtgtcc aatttggaac aacattcaat catgatttgc
     2701 tcttccaact gttctcggac aaagcgagca ttttctgcag aaacaagatt aattttggaa
     2761 acatacgtac cactttgagg tatcgtatac acgagttctt gctgacgtag ttgaatcaat
     2821 gcttcgcgaa tgggagtccg gccgatagca agatgttcct ccagtgcctt ttcagatatt
     2881 ttccttccag gttctaagtc agcatagata atttgtttac gaatcgcttg ataagcttgt
     2941 gattgcaagc tgcttttcat ctttattcct ccaaaatcat ttctatgaac gtagtataac
     3001 agaagaggtc tgaaaaacaa tctggtatgt cacattaact agtaaatttg tataaatagt
     3061 aaaattttgg ttgacatagc gatttttatt gcataaaatt cacttgaata caaaaactaa
     3121 tatgttagtt taggagggga aaaatgattg atttaaacaa ctggaggaac tttatccctg
     3181 aattatctca aaaagaagta gaaatacctc attttgaccg tgcatcatta agagaaaatg
     3241 gacaaaaaaa tcctgtctgg atccatttcg gtggcggaaa tctttatcga ggatttcatg
     3301 cagaaatcgc tcaacgttta gcggatctag gcgaattgtc cactggtgtc attgtttgtg
     3361 aaacattcga tgaagaaata attgaaaggg cttaccaaga ttttgatgat ttgattcttg
     3421 aagtggtcat gccaagcagt ggagagttcg tcaaacgtat attagctgcg acagctgaca
     3481 gtctgtattg tcatccagat tgttctgaac actttgataa gatgattcga cactttgaaa
     3541 atcctagttt acaattagtt acttttgcta ttactgaaaa aggttatcga ttgaaagata
     3601 caaataataa atatatagga ccgattgcag tcgatataaa aaatggtcca aagaagccat
     3661 cacatacaat gagtattctt gctgtgctac tttataaacg ttttttatct ggagcttggc
     3721 cgattgcact tgtctctaca gataattttt cacaaaacgg tagtcaatta cgtacttctt
     3781 tgctaacgat cgcaaaagaa tggcaaaagg aaggttatgt acaaaataca tttatccaat
     3841 acttatcgaa tgaagaaatt gttgcgtttc catggacgat gattgatcgt atcaccccga
     3901 atccttcctt gtcagttagc aaaatgttgg caaaagaggg cttttcaaat atgtcgattt
     3961 tccaaaccgc aaaaggaaat aatattgctc catttgtcaa tacagaggac atccattatt
     4021 taatcatgga agatcatttt cccaatggac gtccgaaatt cgaaaaagct ggggtattct
     4081 tgactgatcg tgcaacggta gataaagcag atacgatgaa ggtgacgact tgccttaatc
     4141 ctttgcatac gtctttagct attatgggtt gtttactggg gtataggagc atagctgagg
     4201 agatgaaaga tccagattta tcgctgctag tttctgaaat cgggtacaat gaaggcctcc
     4261 cagttgtgga gtctccaggt atcattgatc caaagatctt tctggaagaa gtaattactg
     4321 tgcgcttttc taatccaaat attcctaata tgcctcagcg aatcgccacg gatacctctc
     4381 aaaaggtggc gattcgttat ggtgagacga ttaaaaaata tatggaaata gaagttaaag
     4441 acccgcagac acttgtattc ataccattga ctctcgctgc ttggcttcga taccttattg
     4501 gcattgatga tcaaggaaaa ccattcatcc cgagtccaga cccactattg acagaccttc
     4561 agcataagct ttccgatatt caactgggta cgcaagatac atcacatatt catgagaaaa
     4621 tggctcctat tttgtcaaat aaagaaattt ttggtgtaga tctttaccaa gcagagcttg
     4681 gcagaaaaat agaagaaatg ttcatcaaga tggtagcagg accaaatgcc gtacgaaaaa
     4741 cattacagga ttacttacaa gaacatggag gaaatttatc atggaaatga cttttagatg
     4801 gtacggatca caggaagaaa agattacctt agatgaaatc agacagattc ctgcggtcaa
     4861 agggattgta ggcacattga tggatatacc agtaggagag gtatggccta gggagaggat
     4921 caaagaacta aaagaagaaa ttgagtctcg tgggttaaca ctaaaagtaa ttgaaagcgt
     4981 caatgtgcat gatgccatta aaattggatt accttcccgt gagcagtata tcgaaaacta
     5041 taagcagaca atccgaaact tggcagaata tggaatagaa gtcatctgct ataattttat
     5101 gcctgtattt gactgggtga aatctgacct tgactaccgc ttagaagacg gctcatccac
     5161 gttagctttt atatctgatg atattccaga tgatccaaga gaaatcgtcg cgcgcatcga
     5221 acattccagc aatgaatttg gattgccggg atgggaacct gagcgcttag ctcctatcaa
     5281 atccttgctt gaagcctatg catcagtaga cgaagaaaaa cttcgagaaa attttgccta
     5341 ttttctccaa tcaatcatcc caacatgtga agaggtaggg gtcaaaatgg ctgtacatcc
     5401 agatgatccg ccatactcga tttttggatt agctcgaatc gtaaagaatc gagaagattt
     5461 agattggttg tgtcgcatag tagacagccc ttataacgga atcactcttt gtacagggag
     5521 tattgcagaa gatccagaaa atgatgtata tgggatttta gcagaatttt gccaaagaga
     5581 tcgaatccca tttgcacatg ttcgaaatat taaattttta gctaatcgag atttctatga
     5641 ggcaccacat aaatccgaat acggttcatt agacatgtac cgcatactaa aaacaatgta
     5701 tgacaacgga tttaacggat acatccgtcc tgatcatgga agaatgatct ggaatgagac
     5761 aggaagacca ggatacggct tatatgatcg agcattaggt gctgtttatc taaatggatt
     5821 atgggaagca atcgaaaaag gaaacaaaag taaagataat tagtgaaaaa aactgtaatt
     5881 ttattatgta aataagaagg atgttcaaat tcttgataca taaatccgga ggttgttaat
     5941 aagcattcgg atttatgtat cttatttggt ctaggaagta acttgatttt cttttttcga
     6001 ttgatccatg agtgcttctt cttttaattc aaagatatat tctgagatcc tcataatcac
     6061 ttttttccag tcttctggtg tgatgattcc ttttaaaata tagatttttt tgatatgaga
     6121 aggcaattgt ttcaaagggc agtcgctgat aacgatgttg cagttctcca taaaatcatt
     6181 ttgtattttg atcgattcat ttgaaaaaat ttgttctatc ttaacgatca gatctttact
     6241 gatataaaaa tcacgcgtat agttcacacc aatcacaatt cgcggctgtt ttttgtagcg
     6301 atcatacaaa tgtactaggt cttcaaccat ccattgcaca ttttcctttt ctaaaataac
     6361 tggtaacaag tgttgatttt gttctctgaa atccatataa aattgttcaa tggcggtttg
     6421 gacttctttc aatacgctat gttcttgata aagcatgaaa gtaggaaggg gcgtatttgg
     6481 ttgattgaag ttaagaaatc cattataaag tttatttcgt gcgaatgcca atgagtaaag
     6541 aatcttttct ttttcaggta taaacaaatc aaatacagta caaagggact ccaccatttc
     6601 tcttgaataa agaacatgag gaacgtttgc tttttttaaa agtttgtatt gctccaagct
     6661 tgtttcttcc gattcgattt tcgtgataga aagtcgagct aaaatattca ctaagatgcg
     6721 ttgttcgcga caaattggca gattaggtcg caaaatatct acagagagat caataaaaaa
     6781 gtcaatatat tcatactctg tgaattcttt ttgtacgtct ttcacagaag ttgcaggaaa
     6841 attctcttta catattttta atagaaaata taatttatct atcataccgc tatttagtcg
     6901 gtcaagtact tcttttttaa aaaaagtatt catcataaaa gccactactt gcggatcttc
     6961 atcataaaat tctgtagcag tacttgaaaa gatgttccaa taaatatcta atgctgcata
     7021 ttgaatttgt acctcttttc cagaaaaaga ctttctgcca ttattagaaa aggagataga
     7081 caaaccgtac tatgctaaaa atgcattaat ctttttcatt ttcgtataca aataagaggc
     7141 actatagttt gtttgctgac tgaaactgat aatcgaattt gtctgacagc tgaacaaaga
     7201 agtcaagatc tgataactga ctgattcgtt gcagtatctt ccaaatagac gataatagca
     7261 gtaagccgga tcaattcctt caaaattcaa ctttaaaaaa ggtttttgct tatcaatatg
     7321 cagttctgtt agattgagac gctcaaagtc ttgttctaat tctttgatgt atagtaaaaa
     7381 atttgattca gacatatgga aatgatctgc taaatattgt gtagtaataa agaaacgatg
     7441 ttcagatagg aaaagaaaaa tatctaattt tgtattgctt tttttcttca agataaacgt
     7501 tttaatcgct tcttgagttt gcatgaaaga ggctcctttt tacttaaatt gttaaaactt
     7561 gaaaatatta ctgttttttt cataaattta gacaaaaaaa cagtaaacat ataggtagaa
     7621 tctctactat cttatttctt gcatacaaaa aaatcaagga gtgagagagt tatgagatat
     7681 tcaaccatct taatatcaac atttgcagcc ttaagcttat cgcttactat aaatcagaca
     7741 gtccacacag aggagacaga aacagccaaa tggacagaaa ccaagtgggc caacgtcaat
     7801 cgaattacct ttcaggatga attttcatcc ttcaattcag gagtcgcaaa attaagaaaa
     7861 ggacaaacag cgaccatcaa agcaaaaatt gactattcgg gagacttagc acctatacta
     7921 caatcagctg ttacatttga gaatgttgat cccactttgt cgattggcta tgacgagaac
     7981 actttgacta tccaacacaa gcaagcgata tttaccttca ccgttacatt aaatcaggac
     8041 ttgttaaaac cagcgctttt tactgtcaag gtatcagatg gactaccgaa cgatcatcat
     8101 ttaacgccgt atagtcagcg gcagacaatt gagcaagatt cagccatcga ctcaggagga
     8161 gatcttgttg aagaaccaac agataaacca gaaaatgaaa ataagccaga agtaccgcct
     8221 acagaaaatc cagacggaga acagaaaccg gaaatagaac ctggagaaga acccgataca
     8281 gaaactcaac cagagccaga caataaatca aaacctgaaa taacccctgg agaaaaacca
     8341 gatgtaactc cagaaccaga tacggattct ggaaaccaaa cagtaccgga aacaaatcca
     8401 gacacagata atgaaactga aaatccagaa aaaccagagg tagatccaga agaaaaacca
     8461 gatgtgactc cagaaccaga tacggacgca agagaccaag gaataccaga aaaaataaac
     8521 aaaaaaacaa ttcaagagga tggaaaaaaa gagagtaaaa aatcaaatct agcaattcta
     8581 aaaataaatg aagagcagct gaataaaaaa tcacgaatat ttgatagcgc ccaatcagct
     8641 gaaaccttaa aaagtagcaa ggatacaacc tttgctgtcc tgaaacgaaa aataaacagc
     8701 taccaaaatc cggagagtca caaaacaaag ttatactgtg gagcggaatc attctattaa
     8761 gtatagcaac aatgctttct gcaaaaagat ttaaacaaaa cagatctctc taacatttga
     8821 ataatgtctg tgaacgcatt tttcaaataa aaagaaatat ccagaaaatg accccaaaat
     8881 accagattga aatctgatat ttggggtcgt tcatacttta tctttcttca tatttatttt
     8941 aagggatatc tgtttctttt ggaagatggt tcgggatctt tttcactttc aatacatcta
     9001 aaaagaaggt aaagatcgga atgccgacaa tcaagcccca aacgccaaag aaccgttcgc
     9061 taacaagtaa gaccacaaat gtataaaaaa tcggcagttc tgttttgctg gacatcaact
     9121 tcggattcag cacataagac tcaattagat ggacaatcaa aatcgtaatc aggatataaa
     9181 caacatctcg gatgcctcct tgggaataag ctatgaaaga tagaggaata caagaaatga
     9241 taacacctgc tactgggatc agacttaaaa tgaaaatcat gatcgctaaa ctcagcagct
     9301 gatgaaagcc aaatacagcc aaggcgattg tagtgagcaa cgtatttaaa agagcgacaa
     9361 caaattgagc ttctaatacc aaaccaaatg tattgacaaa cttatcggca aaatagtaaa
     9421 tatcttgaaa gaaccaagaa aattcacttt ttaaaaacaa tcttgagaat acaattgttt
     9481 ttttcttttc aatcataaaa aagaaactta agatgaatga catagcaaac gccactgcca
     9541 tttttcctat atcctgtaaa tagccaagcg caatcgaagc gccattttga agctgttcaa
     9601 aaaaatcgtt tcgctcaata taagtatgga tatattgtaa aacaggatct gcatcttttg
     9661 gctgatgctc gtaaaaatta ataacggact ggatcatgtc aaatgtttga tgaataagca
     9721 tcgggatata ctttgtgaat gccagataga taaggtaaac gatcagtgta taaatcatga
     9781 tagctaaaac acctgtaggg attcggacaa atcgctgaat gatttgaaca agatgaatca
     9841 tcaaataagt aaagataaat gtgagcaaaa tggttgtaat catacttcta actaagaaaa
     9901 gaacaaaaac gacaaataat aaaacagtga gacggcgtat tttttcattc gccaaaaaac
     9961 gctgataaac ggtcaaatcg acaacctcca aataacaaaa aataaacacg ttattcatta
    10021 tatgatggaa tatgaaaata agaaagagca aaaagaaaaa ccgctaaatg acgttatctt
    10081 atgagttggt tttcctcata aaatcgttca tctagcagtt tcagcccttt ttctggtctt
    10141 gatgaaaatt atctagcatt tttcgggtca gatgttgcag tgtatccaat tcttcctgtt
    10201 caagtggaag ataagacttg atcttttgtg gaataccttc tgcttgtttt tgcaacgcag
    10261 caccttgagc agtcagcgta acatttacga ttctttcatc acccgtttca cgctgacgat
    10321 taatataatt tttttctgct aactttttta gtaagggagt cagcgtacca gaatccagac
    10381 acaaatcatt tcctagctct ttcactgaag tctttttctt ttcccataaa actaacatca
    10441 caagatactg ggtataggta agattcagcg gcttgaggag gggagtgtag tgtctaatga
    10501 tttctttaga ggcggcatat aaaggaaaac atacttgatt aactaaatat agatcttcca
    10561 tttttccacc tccgctgttt tactatagtg tatcatagcg gatttacaca aaaaagtcta
    10621 ttcactaact gctttcagag taacttctat atttccagct gtagcttttg aatacggaca
    10681 cactttatgc gctgcctcta ataattcttg cgtttcttcc agagaaagac cttcgataga
    10741 tccttcaatc tctacaccta agacaaagct cgtttcactt tctgaataaa gggaaacttg
    10801 tgcactgaca gtagaagccc cagagatatt tttttgctgt ttgatcaatt ctaatgcact
    10861 gttaaaacat gcactgaaac cagctgcaaa caattgttca gggttggttg cattttcgac
    10921 gcgtttacca ggagctgcaa tattcaattc gaatgtacga tcgggattat gcacttctcc
    10981 agagcgtccg cctgtgttga tcattttcgt agaatacatt tttttcataa agttaccacc
    11041 tttaattata ttttatacaa ttgaatttta cacaatataa ttgtggaaag caaagaaatg
    11101 gattggcata agataaataa gagaaagcac atgaaacaga cgcgctttct cgaatagcaa
    11161 acatatacaa aaaatatcag agacaaaaaa taatctcatc ataaacatta catatatttc
    11221 tcaaaaagtt gttttctttc ttcaaaaatt acttcacgat ggacctgatg aagcatccat
    11281 aaataaccaa aaggatctct gaacattgag ttagtcacac caaagtccat catttctgtc
    11341 atttcttgaa tctctacaca gccatttcgt aatgctttat tatgtgtgtc ctcgatatca
    11401 gagacgatga tattgaacca gatcgtattt ggatgttcag cagtaggagc agtcaaacca
    11461 agagacgtgt tttcatccag catatggaat ctcgtaccat acaaggtaaa aatgacttca
    11521 ttattgcctc tagggtaatc tgttgcctca atcagttgta tttcatcaaa gattgtttga
    11581 tacagttcta gtgctttcaa actatcattt aatacaaaat cgatttccgt tcctttatgc
    11641 ataaagatct cctctccttt tcagtcgaac agttactaga ttcttttatt ataccagcta
    11701 aagaaaacgc attcaaaaaa atagcggatt tacttaccaa ctcattcgat tggatgataa
    11761 gtaaatccgc gtcccattac ttctgctacg ctgctgacaa tgacaaaggc acttggatcg
    11821 atttcttcta tcaatggttt cattcgggaa aattcatgat cagtcacaac gatcaataaa
    11881 acctcttttg actttcgatt atacccgcct tttgcctcta gcagggtaag gccacgtcca
    11941 atctcagcac ttaagcgaag gcggatgtct tccaaataat gttcactcat gaccataaca
    12001 gatttctttc gattgatacc tgtttcgata taagtcatga caattgaagt gattgcaata
    12061 gataaaatag cataaagaaa ctcttctatt ccaaaaataa acagattaag agaaacaacg
    12121 aatgcgtctg taaaaaataa gccaatcgaa gtattcaagt ggaaatattt tttaaaaatg
    12181 aggggaggga tcgtcgtacc gccgctagaa gagttgtttt tatataaaat cgcaactcct
    12241 aaagcaaaaa tcgcacttcc aaaaatgaca gacaacaacc gatctgaagt aatcattgtt
    12301 tctggaacta ttgcaatgat taaaggaaaa accaagcttc cgaaaagtac tttaaaaaag
    12361 ggttttttac ctaaaaatag aagagctagt accaacataa cgatattcaa cactaaaata
    12421 actatagctc ggtcaaagcc taaagcagct tccagtaata ttcctagacc gctgacacct
    12481 ccagcagcaa tatgatgagg ccctaaaaac atattgatag cgactcctaa tatgaagagg
    12541 gcaaaaataa tcttaatgta tgtccatata aattgtctcg tttctttcat ttattcactt
    12601 ccttaattca ttaataatac tcttatctta ttataactta cttttcacct taagttaact
    12661 ttaattaata aaaaaattag gctagtctaa attattatgt ttcattcttc tagaatatac
    12721 ttctacgcca aaaactgtta aaaaactttt actttagaaa attggagaaa ttgatcgctt
    12781 aagataaatt caggacacta aatagtacag gaagtagact aaaactttta aaagttttaa
    12841 gtctatctat ttggcaagtt taaaaaataa aaattcggta tcaaaaaact gttaccgata
    12901 acatttttga aagcgttgtc ttttgcttct ctatcactta tagtgatgac gtcatattaa
    12961 aacacaagga ggaaatcaat ggatacagct gcaatttatc atcgaccgga aagtgaatat
    13021 gcatttctat atacgaatac aagatttcgt attcggcttc gtactaggaa aaatgatatc
    13081 aaaaaagtat atgtgctatg tggagatcca tataccataa caactgaaaa atggtatcaa
    13141 aaacaacgac caatgaaaaa atgtctaagt accaatgttc atgattattg ggaaattgaa
    13201 gtgacgagcg aaacaagtcg cttacagtat gcgttccatg ttgttgggga agataacacg
    13261 gactgttttt acggtgatca aggaattttt ccttatcgag aaaatgtgat tactgaacca
    13321 aatttttatt ttcgaatacc ttattttcat caaatcgatc gattcactat tccagaatgg
    13381 gtaaaagaaa cagtatggta tcaaattttt cccgaaagat ttgcaaacgg ggacaaagca
    13441 aatgaccccg aacacacttt accttgggga agtaaagatc cagaccgaga agactttttc
    13501 gggggagatt tgcaaggtgt gatggatcat ttggattact tgacggacct tggcatcaat
    13561 ggcatctatt tttgtcccat atttaaagcc acatccaatc ataaatacga tacgattgat
    13621 tattatgagg tggatcccgc ttttggcgat aaaaaacttt taaaaaactt gattgatgaa
    13681 gcacataaaa gagggatacg tatcatgttc gatgctgtat tcaatcatat gggtgtccat
    13741 tcaccacagt ggcaagacgt tctcaaaaac ggagaaaaat cgatctataa agactggttc
    13801 cacatccgtt ttttcccagt agattcttat cagatgactg acttaccaga aactgctgaa
    13861 aatatcccat atgatacttt tgcttttact ccattcatgc cgaaactaaa tacagcaaat
    13921 ccagaagtgc aaaaatattt gctggatatc gcaacttatt ggataaaaga attcgatatt
    13981 gatggatggc gtcttgatgt tgctaatgaa gtcgatcatc atttttggaa aaaattcaga
    14041 gaagcagtta cagaaatcaa accagacatc tatatccttg gagagatttg gcattcctct
    14101 caagcttggc tgcaagggga tgaattccat gcagtgatga attatgcatt tacagattcg
    14161 attaaagatt attttgcaaa aaagaaaatc acagcttcac aaatggtcag tggaatgaat
    14221 catcagcaaa tgctttacag agatcaggtg aacgaaggaa cattcaactt attagattct
    14281 catgatactg ccaggatctt gaccctttgc caaggtaaca aagaattgat gaagtctgtg
    14341 atggccttta tgtttcttca aaaagggtct ccttgtatct attacgggac agaaataggg
    14401 atgactggtg aagatgaccc ggattgccgc aaatgtatga tatgggagaa ggaggaacaa
    14461 gatctagagc tattcggttt tataaaagaa cttgtttctc taaggaaaca gttgtctaag
    14521 attatttctg aaggttctac tcagtggcta atagtaaatg atcgtgaaga taagctctat
    14581 ttcacacgga aactagaggg acagatcatc tatgtttact ttaatcagtc aaaagaacct
    14641 tgggtagtag aacaggaaaa tgaggtgatt cttagtcaaa attgtcagtt gttagaagac
    14701 gggaaagcag agatacgaca atacggattt ttgatcatgt gttgaaaact gttaccggta
    14761 acaacaagaa agcgtttgca aatgaagatt tgtgcccatt ataataaggg tgtaacagca
    14821 cgttgaatac aaaggaggaa atgaagatga aaaaaagaag tgtgaaacag ttgggtttag
    14881 ggttaatctc tttaggaatc ttaggcggat tagcggcttg tggcaatggt aactcttcag
    14941 cagaagggga agccgatgaa ggaaaaacgt taacagtttc cgttgattct ggatataaag
    15001 attatataaa cgaaataaaa aacactttcg aaaaagaaaa cgatgtgaaa atcaagctag
    15061 tagaaaagga catgttcgat cagttggaat cattagcgtt agatgggcct gctggaaagg
    15121 gacctgacgt tatgatggca gcatatgacc ggatcggtgc gttaggacag caaggacatc
    15181 tggcggaagt aaaactggga aatgaagcag cttacgatga aacagacaaa gcccaagtga
    15241 cttatgatgg aaaaatctac ggagaacctg cagtaatcga gacccttgtt ttgtactata
    15301 acaaagattt agtcgataca gcaccatcaa cgtttaaaga tttggagaat ctatccaaag
    15361 acagtcgttt tgcttttgag tcagaagcag gaaaaaatac aggattttta gctaaatgga
    15421 ctgactttta ttattcctat ggtttaattg caggttacgg tggctatgta tttggtgatg
    15481 atggcacaaa tccttcagat atcggtttga ataatgcagg tgctgtagaa ggaatctctt
    15541 atgcaacaga ttggttcaaa aatgtttggc caaagggaat gcaagacata aaaagtgcag
    15601 gagacttcgc cagccagcaa ttcatgtcca ataaaactgc agcaatcatt gacggtccat
    15661 ggcaagcgca aacatacaaa gaaaacaata tcaattatgg tgtagccaaa attcctactc
    15721 taaataatgg acagccttat caaccgtttg gcggaggaaa aggatgggta gtcagcaatt
    15781 atgctaaaaa caaagacctc tctcaaaaat ggcttgatta tgtaacgaat caggaaaatc
    15841 aagaaaaatt ctttgaaatg gtaaatgaaa tccctgcaaa ccaacaagct agggaaacag
    15901 caaaggctaa aaacgatgaa ttaacaacag ctgtgattga acaatatgaa acagctcaag
    15961 cgatgccaaa catcccagaa atgggcgaag tttggacagg tgcagaaaac ttgatgttcg
    16021 atgcagcatc aggttcaaaa acaccaaaag aatccgcaga tgaagcagta aaaacaatca
    16081 gtgaagccat agaacaaaaa tactgattaa aatgataacc gtccattttt ataaaaaatg
    16141 gaacaggtgc ttcagataaa agtggcaaga gcgagatgga aaattatctt gctcttgttg
    16201 ccattatctt aaaagcagtt tgcggtttag caagtttaga gggggcattc agatgcagca
    16261 agataaaaca agcaatatca acgaaaaaaa aatcacgctt tgttcactta ttccagggat
    16321 ggggcagttt gtaaatggcc aagcttttaa aggtatcgtt tttttcgcca tattcgtcct
    16381 attcattggt cagatgcttc tgggcggatt tcatgcattg gtaggattgg ttactttggg
    16441 aagtatacca atggaagatc attcattatt tattttgatt gaaggcactt tgcaattcat
    16501 cattacaatc attttcttga ttttttacgg tttgaatctt ttagacgctt atcgagtagc
    16561 gaagatgcgt caagagggca aaaaaatcaa tcggacgatg aaggaagtca tacataatat
    16621 aggtgatgaa ggttttcctt atctattgac acttcccgct tatcttctca tgatttttac
    16681 gatcattttc ccagtcctag ttacattgtt cactgcattt actaattatg acttcaatca
    16741 tattccacca gccagtttaa ttgattgggt aggggctaaa aactttttta gtatcttctt
    16801 tttaagctca tatcgtaata ctttttttgc agtcttttcg tggaccgtga tttggacttt
    16861 atgtgcaaca actttgcaaa tcacccttgg tattctcaca gcggttgttg ctcaccagaa
    16921 atttattaaa ttcaaacgta ttttcggtat cattttctta ttaccatggg cggtcccagc
    16981 ttttattact atcatgagtt tttcgaatat attcaacgat agcgtaggtg ccatcaatac
    17041 tcaagtgatt cctttcataa accagcttcc ttttgtagat atcagtgcaa tctcttggaa
    17101 aacggacccg aactggacga aagtagcaat tatcttagtg cagggctggt taggatttcc
    17161 ctatatctat gtgatggtta caggaatctt acaatcgatt tcagaagact tatatgaagc
    17221 agcaaaaatc gacggagcaa atgcatggca gcgcttttca aacatcacgc ttcctgctgt
    17281 cttcctagtg gcagcgccaa catttattac acagtatacg gggaatttca ataatttctc
    17341 catgatctat ctgtttaaca atggcggtcc cggaagcgta ggcggaggag ctggttctac
    17401 tgatattctt atttcctgga tctataaact tacgactgga acttctccgc agtattctat
    17461 ggcttcagca atcacgttga ttatctccgt catcgttatc tgtgtgtcat tgctggtatt
    17521 taaaaagaca aatgcgttca atatggggga ttaacaatga aaaaatttca ttcgagtgct
    17581 cgattccaaa aacgactttc tcactttttt acttactttt ttcttttaat actgtcagtc
    17641 attatcatct atccgctttt gatcacagca agtactgcct tcaagtcagg aaatattgca
    17701 gcttttagtt tagatctttc aagtgactgg actttaggaa actttactcg gctattcagt
    17761 gaaacacttt acgggacttg gtataaaaat acattgatca tttctgtcct aacaatgatc
    17821 gtacaagtga ctgtagtaac tttggcgggc tatacttata gtcgctatcg ttttatcggc
    17881 cgcaaatcaa gtctgatgtt ctttttagtg attcagatgg taccgactat ggcagcattg
    17941 actgcctttt atgtcatggc tttcttatta gaagcgctcg ataagtactg gttcttgaca
    18001 atgatctata taggcggtgg aattccaatg aatacttggc tgatgaaagg atactttgac
    18061 actgtaccaa tagatttaga tgaatctgct aaacttgatg gggcagggca tttcaggatt
    18121 tttttccaga ttgtattacc gcttgtcaaa ccgatgctcg ccgttcaagc attatgggct
    18181 tttatgggac cttttgggga tttcatgtta gctaagttcc tgcttcgaac acctgaaaat
    18241 cagactgttg ctgtcggctt acaaacattt atttctgatg tgacaaacca aagagtctct
    18301 ctttttgctg ctggggcaat tttgattgca ctaccgattt gtctattatt cttcttttta
    18361 caaaagaact ttgttaccgg cctgacatct ggcggaacaa aaggatagga gcagagtatc
    18421 atgcaaacat ttccgttaaa ctatttttcc gcattacgta cgccaactca agtgtttgcg
    18481 ggacgcaaac tattatcatg gcctaaattt ttcttgatat ttgtattttt agtttccttg
    18541 atggtcatgc ctgtgactct tttttatgcc aatcagatcc aagcgattcc attagaacaa
    18601 tttctatccg ttcattcgct gatcgatgaa caaggaaccc aaaaattttc tgaacttgaa
    18661 ttatctgaaa ctgggttaca agcatcgcaa caaacgatag ctgtgacccc tgaaattttg
    18721 gtaggtgtgt ctttatctga gaaacaacaa tccgatcatg gaacttttat tgattttgag
    18781 aaggaacaat gggtgatcca acaaaaggat aaatctggta tacgccgcta tacgatgaat
    18841 tatagcccat ctttccaacc agattctgta cgtacgcccg aagatttcca acgattttta
    18901 gaaagagaat tttatgccag taatcgtcca acaatcattt tatcttatag tttaagtctt
    18961 ggtcttgtcc tatttgtcat gacgagtctc attttattcg gggcttcctt tttcttgtgg
    19021 atgacaagaa aaagccaact ttcatccatt catacattca aagagagtgc aaatttgatg
    19081 ctgaatgtta tgggaatagg ttccatggca gcagcagttg tagggtttat ccactttgac
    19141 tttatcttga tgctaagtgt ccaaacaacg ataactgtct tgctgctttt atgggttttt
    19201 gtcaaaacaa gattcaaaga taagcgtgtc gaatgacaag tttaagcgat ttatgcgaca
    19261 atgaatgtga aaaaaatgaa aaataaagta gtttttgaaa aatttctgaa aaaattaaac
    19321 ttcttagttg attcttttct tttttagtat taacgtaata gggtagtttt tttaaggagg
    19381 aaaatcatgt tagagaaaac tttatacaat caattatttt cacgttcttt ctcgctccct
    19441 gtcgaagtaa attattggga cggtacaacg aaacaatacg gagatacgga cactcctccc
    19501 caaatcaaaa tcaatatcca tgaagaaatc ccaatgaaag agattacaag taacgcatct
    19561 ttggcactag gagaagccta tatggatcat cgaatcgaga tcgagggaag tatccaagca
    19621 ttgatcaacg atgcttatca gcaagcaaac agctttttac gaggaaaaga ttatctaaaa
    19681 tggctgccaa agaaagaaaa acatacgaaa caaaaaaaca aagaagatat ccatgcgcat
    19741 tatgacttag gtaacgactt ttataaactt tggctagatc caactttgac ttattcttgt
    19801 gcttatttca atacacctga agatacgttg gaacaagcac aaatcaataa agtccatcat
    19861 atcttagaca aactctttat caaagaaggc gaaacgttgc ttgatatcgg ttgtggctgg
    19921 ggaacattga tgttcactgc taccaaagag tataatgtca aggcaactgg gatcacacta
    19981 agtgaagaac aatacgattt gattaccaaa agaataaaag aagaacattt agaagataaa
    20041 tgccgtgtat tattaatgga ttaccgcgaa ctaaaaggcg aaacgtttga tcatatcaca
    20101 agtgtaggta tgttcgaaca tgtaggttct gaaaatcttg aaggttattt caaagtggta
    20161 aaagatttat tgaaaccaaa aggaacagct ttgatccatg gaatcagccg acaacaaggc
    20221 ggtgcgacaa atgcttggat caataaatat attttccctg gcggttatgt ccctggtgtt
    20281 actgaattag ttgataatat tacaaaaaac gatttgcaac tgattgattt ggaaagctta
    20341 cgtcgtgact accaattgac attagaacat tggacaaaga attttcatca agtccaagat
    20401 caagtcacga aagaaatggg tgattccttc tatcgaatgt gggatctata tttacaagct
    20461 tgtgcagctt catttgaatc aagtaatatc gatgtgattc aatatttgct tgttcaacct
    20521 ggaaacaatg acatcccaat gcatcgtgca taattgaggc aaaagtactg tttaaagact
    20581 atttaaaaag aagctaagtt ttatgatgct ccagaagtga acttttttgt ctaacttcta
    20641 ggattgtctc taaagcttag tttctttttc ttt
//
LOCUS       DAZBTF010000052        20380 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000052 DAZBTF010000000
VERSION     DAZBTF010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.052"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..377
                     /locus_tag="ACHGNS_001926"
     CDS             <1..377
                     /locus_tag="ACHGNS_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937857.1"
                     /translation="DVESQQFGTNVINKVINPALSINAPLIPTALSFSVTAVVSEIED
                     TEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLVEEAGEHYAVVK
                     FNGTEIGRQIFDIKVNKPVEKN"
     gene            391..792
                     /locus_tag="ACHGNS_001927"
     CDS             391..792
                     /locus_tag="ACHGNS_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937858.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            794..1165
                     /locus_tag="ACHGNS_001928"
     CDS             794..1165
                     /locus_tag="ACHGNS_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937859.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1201..1500)
                     /locus_tag="ACHGNS_001929"
     CDS             complement(1201..1500)
                     /locus_tag="ACHGNS_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFN6937860.1"
                     /translation="MLFNETQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(1506..1736)
                     /locus_tag="ACHGNS_001930"
     CDS             complement(1506..1736)
                     /locus_tag="ACHGNS_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937861.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(2172..2738)
                     /locus_tag="ACHGNS_001931"
     CDS             complement(2172..2738)
                     /locus_tag="ACHGNS_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN6937862.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(2738..3694)
                     /locus_tag="ACHGNS_001932"
     CDS             complement(2738..3694)
                     /locus_tag="ACHGNS_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFN6937863.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(3694..4350)
                     /locus_tag="ACHGNS_001933"
     CDS             complement(3694..4350)
                     /locus_tag="ACHGNS_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFN6937864.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            4720..6738
                     /locus_tag="ACHGNS_001934"
     CDS             4720..6738
                     /locus_tag="ACHGNS_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFN6937865.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    6808..7014
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7165..9579
                     /gene="leuS"
                     /locus_tag="ACHGNS_001935"
     CDS             7165..9579
                     /gene="leuS"
                     /locus_tag="ACHGNS_001935"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFN6937866.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            9927..11480
                     /locus_tag="ACHGNS_001936"
     CDS             9927..11480
                     /locus_tag="ACHGNS_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFN6937867.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(11861..13645)
                     /locus_tag="ACHGNS_001937"
     CDS             complement(11861..13645)
                     /locus_tag="ACHGNS_001937"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6937868.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(13660..15381)
                     /locus_tag="ACHGNS_001938"
     CDS             complement(13660..15381)
                     /locus_tag="ACHGNS_001938"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6937869.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            15481..16242
                     /locus_tag="ACHGNS_001939"
     CDS             15481..16242
                     /locus_tag="ACHGNS_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFN6937870.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            17209..17477
                     /locus_tag="ACHGNS_001940"
                     /pseudo
     CDS             17209..17477
                     /locus_tag="ACHGNS_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            18149..>20380
                     /locus_tag="ACHGNS_001941"
     CDS             18149..>20380
                     /locus_tag="ACHGNS_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFN6937871.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
BASE COUNT         6752 a         3554 c         4045 g         6029 t
ORIGIN      
        1 aagatgtaga aagtcagcaa tttggcacta atgttattaa taaagtaatt aatccagctc
       61 tttctattaa tgcgccttta attcctactg ctttatcatt ttctgtaaca gctgtggtgt
      121 ctgagataga ggatacagaa aagataaaaa tagtagaaat tgaagtttta aacaagaatg
      181 aaaaacaaat tttttctacg ggtgaagtat cggtaagctt gcctcctcaa gttaacgata
      241 taaactttaa tataaacgcc agaaatgtct tggttgaaga agcaggagag cattatgctg
      301 ttgttaaatt taatggaact gagattggaa ggcaaatctt tgatatcaag gtcaacaaac
      361 cagtggaaaa aaattaagga gacgatacgg atgcttgata tagtaccatc aaatacacat
      421 cggagtggta gcttaataaa ttttgctgcg atttttgctt gcgcatcggt aattctagca
      481 tctccagttg ttgtggatgc aagttctact ccgaaaacta aaaatgataa tcaacttgta
      541 attactacaa aaaatgagat taacacaatt tcaagtaatg atggcaaaat ttttgatgtg
      601 ataaatacgg ttttaaaaag aaaagctgaa atcaatataa aatatgatga agatttaaac
      661 ttgtatttct ttgttatcaa aacaacatct gaattgtttt cctcagatta cgatgttttg
      721 gatactctcg ataatgtatt atctgactat aaatatatgg ggaaatctgt agtagcaact
      781 ttgggggaat aaaatgtttg actttaatga atatttatct atttccaaac aaattgaatc
      841 agatgataaa tataattcta aagaatctta tagaagaact gctgtttcaa gagcatacta
      901 cagtgctttt aaaaaatctg atgagtattt gaaagaaaat tatgatatta tttataatgg
      961 aagtagcgga aaaggaagcc accaaaccgt ttggaatttg ttttcaacag ttaaggaatt
     1021 gaatacctta ggaatacata atagtgggta tagaatgtta gagaaaagaa aatgtgctga
     1081 ctatattcct aatgaaacca ttacgaaaac agatatggcg ctgatgaatc gagaagcaga
     1141 aaaaataata aacaaactca cctaatgaat ttcaagcgag tttgtttgtt atttttttat
     1201 ttaatctttg tatctttctg gatcaacgaa agtatacttt atatagtcat aacgccgatg
     1261 atcgctacgt gcgtccggca cgtcagtcac gatatcaaac aaaaagtata cacccttctt
     1321 cattctagtt ttcgcagcag gaattttgaa atagttctta ttagaatagt agagattgat
     1381 taataagcta tcttcgattg ctaaaaagaa aacttctgaa ttccatactt tataaaaatc
     1441 tttgataaat ctattcgaag ggtcaaattt aaaccataat tgtgtctcat taaaaagcat
     1501 aaccattact ccgatctgtt tttaaaatta gtttctacct ctaatatata tcgagttttt
     1561 attttgcctt cagagaatac tgtttctttt tttgcagtta caggttgttt attttcggaa
     1621 aaagctaata tagctaagat tgaaacatcc atctggaatt tatctttttt actgctttga
     1681 tcataaaagt ctgcatattc atcactgata ttttttctaa taaattcttc catcataaaa
     1741 atcacctcaa aacgattata cgaacttacg ttcttgttgt aaagcgatat ttgaggggca
     1801 aaaaaggggc ataatttgac tgatattaag ttagctattg atatatgaat gacatacaaa
     1861 aatataaaat cttttaaaaa tgcttatata tcaatgtttt tgccattctt ttatatatct
     1921 gttatatgtc aaaaaaatgt gttcaatttc aatttgtatt tgaataaaga ggttgtgaaa
     1981 aagctgtcct gcatcaagta ataagaacag ccaatcaaaa acagcttctc atgtttttga
     2041 tttggttgga cttattaccg cagatataag cttttgagca ccgtgaataa agaggttatg
     2101 aaatcaacgc tctaacctga gtattagaga aaaaaacgca aaacatatca tttatgtttt
     2161 gcgtttttta tttatctttt tttctcaata caatataaaa taggcggctg gttcttttgg
     2221 ttgataaacc gataattcag cacattatat gtttcctgtg gcagtttgga acaaaaagaa
     2281 tcaaccatat ccagttcttt ttctccacct tcatgaccat agtagacgac aaggatcaat
     2341 cgtccgcgag gcacaagtcg tttcaatatt tcttccatag ctgtgcgtgt agtctctggc
     2401 atagtgatga ttgctttatc actttttggg agatacccta gattgaaaat ccctgctttg
     2461 agattttgtt gttcagctat cacttctcct aacgtttcgt ggccttgtag aaacaatgag
     2521 acacgttctt ctaactgacg ctgttctagc ctttcacgag tagcatcgat tgcttgttgc
     2581 tggatatcaa aactatagac atgaccagtt tttccgatgt gttctgctag aaaaaccgta
     2641 tcgtggccat tgcccatcgt tgcatctacg acgtgatccc ctggttgcaa gatttcttgc
     2701 agtaatgtat ggctgaaatg caaagcagtt tgcaacatca gacgctcacc tttttccgta
     2761 catcatattt tccttggaag ctatctctgc gaagaagttc ttcatcaatt gcattcaaga
     2821 cttcccattt tttcaagctc cacattggtc caatcaagga atcccacggc gcatcccctg
     2881 tcaaacgatg gatgatgatc tcttttggta tcatttccag ctggtcgcaa atgacattga
     2941 catagtcagg acggctcatt aattgaaggc gaccttcatg atagtcccgc aacattctcg
     3001 ttttgcgcat cagatgcatc aaatggagct tgattccttg gatatctgaa tctaaaatgg
     3061 tgcgtctgac attttctttc atcatttcta aactttcgcc tggtaatccg ttgatcaaat
     3121 gcgtacaaac acggatatta tgtttgcgta atttagccac gccatctaaa taagtttgat
     3181 aatcatgtgc tcggttgatc agttttgaag tttcttcaaa agttgtttgc aatccaagtt
     3241 ctacccataa atacatccgc tcgttcagtt cagcaaggta atcaactact tcatctggca
     3301 gacaatccgg tcgtgtaccg actgataatc ctacgacacc tggtaaattg actacttgct
     3361 caaatcgttc tttgattatt tcaactggcg catgggtgtt tgtaaaattt tggaaataaa
     3421 cgatgtattg ttggacatga ggccattttt gatgcatcat gtcgatttct ttctggaatt
     3481 ggattggaag agggtcttca ggtgcaacga tcatgtctcc cgaaccagat acactgcaaa
     3541 aagtacaacc gccatgggca acagtcccat ctcgattcgg acagtcaaat cctccatcga
     3601 tcggcacctt gaaaattttc ccgccaaact catttcgtaa agcgtaattc caagaatgat
     3661 atcttttatt tccttctgaa taaggaaaag tcattaaaag tccttctttc tgtaagagtt
     3721 atctatttgt tttctttttt ctctatacac tggataagtc aggcacagcc aagaaagact
     3781 gagtaagtaa ccgccgatga tatcagtagg aaaatgaacg cccagataaa tgcggcttat
     3841 cccgacactt actataataa acatcatcag caattgaagc aatgtgcgta aggttctttt
     3901 ttgaacaaaa gctggaagca gaaatagtaa agaaccgtaa agaatcatac tgcctgctgc
     3961 atgtccacta gggaaactgt aacttgtttc aactactaag tgcgccaatg aaggacgctc
     4021 tcgcatgaaa aatagtttga tcaccgggtt gatgattcca gcaattacaa taaatccaga
     4081 aacgaaccaa aatgtttctg cccttttttt cttataccac atccataaag ccgcacaagc
     4141 aaataaaatg atgacggtaa gaggattacc gaatttagtc ataaagagtt ggtagctgtt
     4201 cagatcagga taagaagctc tgacaatgcc tgtaaaccac tgatcgaaac cagaaagcca
     4261 cgacagatgg aactttacga tccagcccaa aatgaaaaac aggacaagaa agaagctacc
     4321 agccacctga aatgatgttt tatatttcat acaattcctc ctttgaagca atgacttatt
     4381 atagcatttt tggtccagcg aaaaaaacgg taaagagata aaaccaatgt atgattcctc
     4441 tttgaataac caatattctt ataaaaaaaa taactgtaat cggaaagaca ttttttggta
     4501 atgcaaatga aatgttaggg aaataaaatc agtgtttttc gaaatccttt taaacaagaa
     4561 cgtagacgta ggaagaactt gaaaatgaac agaagttgtt cattacggta aattacaata
     4621 tgattacaaa aaacaacaaa gctcttaaat aacttttaat aaatattaag agaagttata
     4681 attattaata atagataaat tgaagttgga ggatagaaga tggaaaacat tgcaagaaaa
     4741 gaacggagac gtttagaaca gaccaaacgt tttcgaaaag caaaacgagg ggcagcaatc
     4801 gtgggaactg cgatggtagg ctgttctgtc gctgccccat tagtacagcc ggttcctgtt
     4861 caagcggatg aaacacctgt tcagttcggc gcaagaatca atacagcggc atttatcaca
     4921 gaaattgcta cttatgctca accaatcgct caagccaacg atctatatgc atcagtgatg
     4981 atcgcacaag cagttgtaga aagcggttgg ggcagtagtg cgttatctca agcaccttat
     5041 tataatcttt ttgggataaa aggcagctat caaggacaaa ccgtctacat ggatacgctt
     5101 gaatttttaa ataatcaatg ggtaaataaa aaggagcctt tccgacaata tccttctttt
     5161 gctgaatctt tcaatgacaa tgcctatgta cttagaaata cttcatttgg caacggttat
     5221 tactatgccg gtacttggaa gagcaacacg aaatcctata cagatgcgac agcttgtttg
     5281 acagggcgtt atgcgactga tcctggttac gcaggcaaat taaataatat catcgcaact
     5341 tacggattga cccaatatga tacaccagct agcggaaacg caggcggtgg agcgactaca
     5401 ggtggcaata atactggaag tgcaggtagt actggcggtt caagcaattc tggtaataca
     5461 aactcaggtt cagcaacttc gaccacttat actgtccaat ctggtgattc tgtttgggga
     5521 atctcaaata aattcggtat cacaatggat caattgatcc aatggaacaa catccaaaac
     5581 aatttcattt atccaggaca aaaactgaca atcaaaggta gccaggaaaa tggctcttcg
     5641 acgaataatt ccggtaacaa taccaattct tcaggaaatg ctggtacatc aaacggagga
     5701 caaacaactg gtgcgaaata cactgttcaa tctggtgatt ctgtttggaa gatctcaaat
     5761 gatcacggaa ttacgatggc tcaattgatt gaatggaaca atatcaagaa caatttcgtt
     5821 tatccaggac aacagctaat cgtatcaaat ggcggttctt ctaatgctgg agcaaatact
     5881 ggctcaaata ctgggaatac aagtggctct agttcgtcca gctcaagttc ttctaataca
     5941 gcttcaggaa cgaaatatac agttaaagcc ggagactctg tttggagcgt ctcaaataaa
     6001 tacggaatta cgatggctca gctaatccaa tggaacaata tccaaaataa ctttatctac
     6061 ccaggacaac aactgactgt ttcaaacagt ggatcaacaa acagcacgtc aggtaataca
     6121 ggttcgtcaa ccaatacaaa taacagcagt tcagttaata ctggcagcag ttcgtctcaa
     6181 ggcacttata ccgtgaaagc aggtgaatct gtttggagcg tggcaaataa aaacggcatc
     6241 acgatggatc agttaatcga atggaataat atcaaaaaca acttcattta tccaggacag
     6301 caattgatcg ttaaaggtgg aacttcggca aatacaaata caggttcaac tacttctgca
     6361 gcgaaaccaa atacaccgaa tacgtctgct acgacaagta catcatccac tggtaatacg
     6421 atgtataccg tgaaagcggg cgagtcagtt tggagcgtag cgaacaaaca tcatatcacg
     6481 atggatcagt taatcgaatg gaataatatc aaaaataatt tcatctatcc aggacagcaa
     6541 gtgattgtga agaaaggttc atctcaatca acaaaccaac aatcagttac tggttcgaaa
     6601 acctatactg tgaaatctgg agaatctgtt tggagtgttg ctgacagtca tggaattaca
     6661 atggctcaac taatcgaatg gaacaatatt aagaacaact ttatttatcc aggtcaaacg
     6721 ttgattgtag ctaaataaaa tagctgttcg aagaaataac gattgaattc cacaaaaata
     6781 tttgttacta taaggctact taaatatgac aacacttgaa aaggaagtag taaaaagact
     6841 gaagtttcag agagcgcatg gctggtgaga atgtgcacgg atgcttttga actcgccttt
     6901 gagttgccct tgctgaatac ccgagtaagc aagcggcggt gacggccgtt acaacgttcg
     6961 aggccttttc actaaggtaa aattaggtgg taccgcgtag attacgtcct ataaaaagag
     7021 caatcttttt ataggatttt tttatttctt ttgtgaagtt tgagaaaaca aagcaaaagg
     7081 aaataaaaat aaacagattc tgccggtttt ttcgttatat ttataataga aacaagtgtt
     7141 ttcaatagaa caggaggaca gcaaatgagt tacaatcata aagaaattga gaaaaaatgg
     7201 caaaaatact gggcaaaaac gaatactttc aacacccatg atgatcctga aaagccaaaa
     7261 ttttacgcac tggatatgtt tccctatcca tccggtcaag ggctacatgt agggcatcct
     7321 gaaggttata cggcaacgga tatcctttca cgtgtgaaaa gaagtcaagg atacaacgtg
     7381 ctgcatccaa tggggtggga tgcattcggt ctgcctgctg aacaatatgc gctagataca
     7441 ggaaacgatc cagctgagtt tacccaaaaa aatatcgaaa cattccgacg ccaaatcaat
     7501 tctttaggat ttagttatga ctggaatcgc gaagtgaata caactgatcc ggagtattat
     7561 aaatggacac aatggatctt tacgaagctt tatgaaaaag gtctagctta tgaagcagaa
     7621 gtagcggtaa actgggtacc tgaattagga acagttattt caaatgaaga agtgatcgat
     7681 gggaaaagtg aacgtggtgg ctatgatgta gtcagaaaac caatgcgcca atggatgctg
     7741 aaaatcactg cttatgctga tcgcttattg gatgatctag aattagttga ttggccagaa
     7801 agcattaaag aaatgcaaag aaattggatc ggtcgttcag taggagcaaa tattgaattt
     7861 aaagtggctg gtacggataa atcctatacc gtatttacga cacgaccaga tactctattc
     7921 ggtgcaactt actcggttct tgcacctgaa ctagatcttg ttagagaaat tacgacccct
     7981 gaacaaaaag ctgctgtaga agcttatatc gaagagacag ctaaaaaatc agatctaaag
     8041 agaacggatc ttgcaaaaga aaaaacagga gtatttactg gtgcttatgc aatcaatcca
     8101 gttaatggca aggaaatccc aatctggatc gccgattatg tattatcttc atacggcaca
     8161 ggagccatca tggctgttcc agcacatgat gaacgtgact ttgaattcgc tcaaactttc
     8221 ggactagaaa ttcttccagt tatcgaaggt ggagatgttg aaaaagctgc ttacacagaa
     8281 gatggtacgc acatcaactc tgaatttcta gacggcatga acaaacaaga agcaatcgat
     8341 aaaatgaatg aatggctaga agaaaatggc gtagggaaaa aagaagtcag ctatcgctta
     8401 cgtgactggc tattctcccg tcaacgttac tggggtgaac caatcccaat catccattgg
     8461 gaggacggaa cagtcacagc tgtcccagaa gaagagctac cattacggtt gcctaagaca
     8521 aaaaacatca agccaagcgg tactggagaa tctccattag caaatatcga ggagtgggtc
     8581 aatgtcgttg atcctgtaac aggtaaaaaa ggccgtcgcg aaacgaatac aatgccacaa
     8641 tgggcaggaa gctcatggta ttacctgcgt tatatcgacc ctcataacaa aaaagaacta
     8701 gcagattatg aaaagttgaa gcgttggcta ccagttgaca tctacattgg tggagcagaa
     8761 catgcggtac ttcatttact atatgcacga ttctggcata aattcttata cgatatcggt
     8821 gttgttccta caaaagaacc attccaaaaa ctatacaatc aaggaatgat cttaggcgaa
     8881 aacaacgaaa aaatgtcaaa atctcgtggt aatgtagtca atcctgatga tgtggtcgaa
     8941 acttacggtg cagatacact gcgcatgtac gaaatgttca tggggccatt agatgcttcg
     9001 attgcttgga gcgaaaatgg cttagaagga agccgtaaat tcttggatcg tgtatggcgt
     9061 ttgatcgttg atgagaacaa taaaatgcgt gaccgtatca ctacattgaa tgatggcaag
     9121 cttgataaag tgtatcatca aacagtgaaa aaagtgacag aagactatga aaatcttcat
     9181 ttcaatacag caatctccca attgatggtc tttatcaatg aagcctataa agtggacgct
     9241 ctgccttatg aatatatcga aggatttgtt caactactcg caccgattgc tcctcacatg
     9301 ggtgaagaac tatgggcaat ccttggaaat gatggtggta tttcttacgc accatggcca
     9361 acatacgatg aagcagcact tgttgaagac gaagtagaag ttgttttcca agtaaacggt
     9421 aaagttcgtg cgaaaagcaa tgttccccga gacttaggaa aagatgagct agaaaaagca
     9481 gcattagcca atgaaatagt gcaagaatat attgaaggca aaacagtcag aaaagttatt
     9541 gcggtaccaa acaagttagt caatatcgtt gctaattaat tggctctttg tcaataagga
     9601 ctgatgagtt gtgcaaaatc aaatctgggc cagaatgaac ttcattctgt ctcagatttt
     9661 ttgttatttc aaattgatta attgattgat caaaaaggtt ttgattttca tgttctcaaa
     9721 cacgttcaat gtctttttct tctgaatggt cttgtaagag atctctgtaa tcatcaaatt
     9781 tttgtctatt aatcttagta ttttttgatg tataaactac ctgaaattga gagtacatac
     9841 caaattcata aattttttat tagtgggact agtgattgta tggattttga cgcataatga
     9901 atgaagtatg agaaaaggag agacaaatgg atcaagaact atttattatc gaagtattat
     9961 cagctaaaca aaaaattatc gagaaagcga aaaaaagagc gaaaagaata atgttcaagc
    10021 ttttaagctt agtatttttc tctggtttgc tttttttcgg agctgtatcg ttcctatatc
    10081 gccatatgag tgcagtagtc cgttcagctg aagggatgca aaaattaaat aatttgtatc
    10141 acttttcgtg gggagttggc ggaataacaa ttgttgtgct tattgttttt cttctactta
    10201 tttggctccg ttaccatcaa ttgtcgcgtc atttcgatca atggcttagt caaagagcac
    10261 aacagttttt acataataaa ccaacttctc aagatgatga ctatctttat tttgagaaga
    10321 cttcgacgag tcaagctttt cggatacaaa aaagtgattg ccagttattg ctgagatcac
    10381 ttgatgattt acctatctac ttaggcgaaa gcaaatcaaa attcggaatg aacacattcc
    10441 atattttttt actagaaaac gaacccagat gggaaaaaag ccagtcttcg cctggtggat
    10501 acctgacaac taagatgagt acattagtca ctggtttttt cctcattctt ttagctggaa
    10561 caatctatta tgggagatac actcagccaa aaatgtcttc tcatataaat gctgcttctc
    10621 acatgacaat gaactcaact aacgatacgc atatacaaaa gacaaatgaa gggttgttag
    10681 ctgatcctaa gcaaaaggta aatttgacaa cacaaaagat aaatgatctc aaacttgata
    10741 cagagacgca ctctctctat atgacaacag atagtggtgc taattgggta tttgtgccga
    10801 ttgattcgca atggttgcgt tttggcgaat acaccctgac aacggggctg attcctgaag
    10861 gttattggat ggacaacaca tatgatattt ccgccgattt ctcatggttt atctattctt
    10921 ctggggatga tgtacgtctg ctgacttcaa gagataatgg taaaacatgg caaacgtcta
    10981 ttatcaccca atcagttcag ccgatacgtt atcgaaaagc ggtattttat ggcaacgatg
    11041 gtctatttat ctgtacatca gccagtggga tgtctgcaga aactttgtac atttacacta
    11101 ctatggatgg cggaatcact tggataaaaa gtggaagtac aatgatcaat cagccagtac
    11161 aaaatgtcag cttcgttaca acttctttag gttttgtttc gacacgtgaa agtttgtttt
    11221 atacaaacaa cggaggacgc tcattcaaag aatcagttct gtcgattccg gaaaactatc
    11281 agacaggggg gatagatctt tttcaatcac caaatgaagt tactcaaata agcgcgaata
    11341 cgttagagac aaaattttat ttgttgaaaa aaggaccaat cgatgcaggg aaaatgtttg
    11401 cctgcctctt ccgttcaact gataacgggg aaacatggca attcgttcag caactatcgc
    11461 aagtagaaca aggagattaa agaagagaga ttgtgagcct gacgttcagc accgagtcac
    11521 aagaaacaaa ttcaaaaaac agcttctcat atttgttgaa tagtgtaact cgtgattcgg
    11581 agttgacaag agcaacaaga agcgattttg aagtatagct tctcgtattt tacaagatcg
    11641 tgtcttattg ccgataggtt gtctcttgaa caccgtttat tcgtaaaaag ggattgtgac
    11701 aagtcttttg tcactactta ccgaacgaaa ctgaaggagc tatccaaaaa gtatcattag
    11761 tattattctt tgtgtttcta gtggcaccac tagtacattt aaaaaagcta tcgatcatgg
    11821 actaatcata catgatcgat agctttttag ataatgtttg ttacgctatc tgaaattgac
    11881 tattataaag actggcataa agcgttggtg attggagtag ttcagaatgt gttccttttt
    11941 cgacgatatc gccattcttc attacaagga tcatgtctgc attttcaatc gttgacaaac
    12001 ggtgtgcaat cacaaaactt gtcctatctt ttgtcacagc ttccattgct tgctgaatca
    12061 atgcttctgt tcgtgtatct acgctagaag ttgcttcatc taaaataacg acaggaggat
    12121 tcgctagaat gactcgagca attgtcaata attgttgctg tccttgagat aacgtgccgt
    12181 tttcgctaga aatgatcgta tcatatccgt ctggcagtgt tcgaataaag tgatcacatt
    12241 gtgcgatttc agccgcttcg ataatttgtt ctcttgtggc atccattttc ccgtaggcga
    12301 tattatcagc taccgttcct tcaaacagcc aggtattttg taaaaccatc ccaaaagaac
    12361 gacgtagttc tccacgtgaa agcttggtaa tatcaagacc gtcgaattcg atctttcctt
    12421 tattcagttc ataaaaacgc atcagcagat tgactaaagt cgtttttcct gcaccagttg
    12481 gaccaacgat cgctaccgtt tgttttggct ttactgaaaa atcgacttga ttcatcaata
    12541 ttttatctga tgtgtaacca aactgtacat ttttgaaagc tatttttcct ttcggttgtt
    12601 ctaaaagtct tgcatctgga aggtctggaa tttcatctgt ttcatctaat atctcaaata
    12661 tcctatcgat tgctgccatt gcggattgga tcgagttgat cacataagaa gcggtagaaa
    12721 ttggttcaga aatctgattg atgtactgaa gataagcttg taagaagccg atcgtcacac
    12781 cacctgatag aacaagcata gcaccgaata ccgcacttat gatgaaggcg agttgattaa
    12841 tgaaacgaat ggccggatag atagcacagt tcataaattg agcacgttta aaagcgttgt
    12901 attgcttttt atttacttcg tgtatagcag cttctgcatg agcttgctga ttgaatgttt
    12961 tgaccactag attaccagct aagatttctt ccattcgatt atttaaaaca cccagttcat
    13021 actgattctg ttcagaaatc tgtttgtttt tgttcgcaat cttagttgta acaattgaac
    13081 tgccaacgat cagcaataag acgagcaaag taagcttgct ttcgatataa aacagcatca
    13141 gaccagcagc agctattgtt acgacagaag taaaaaattg attgatacct gtcaataaca
    13201 cttgagaaag ttgattgaga ccggtagttg tccgactaat gatgtcacct acttgatgat
    13261 tgtcaaaaaa cgacatcggt aatgttttga atttttttgt gatttctttt cgcaccctca
    13321 atgtgatctt ttcgcttaac gctgccattg tccgttcctg aataaatgaa gtgatgctgc
    13381 ttaagactga acagccgatc agcaataaga cagggaacaa caatgcttgt ttgatatcac
    13441 tgaaagaaag ctgtccaata cctgtttttc ttatttctga taagagatca tcgatcccga
    13501 ttcccatgat caagggcatg gctacgacta ataggttccc gaataaactg catagaaaca
    13561 atccgtaaaa atatttatgt tcaggtttga tcattttcca aaaacgtatc aaagcacgcg
    13621 tgttcaacgt ttttttcttt tccatcgtat cccttctttc tatattcctt gtgattttgc
    13681 aaactcctga taagtttggc aattagacag tagttcttca tgagtacctt gtccaacgat
    13741 ttttccttca tctaaaacaa tgatgttatc tgcttgttgg attgtactga ggcgttgagc
    13801 aacaatcacg agagttttct ccgacatgtg tttatgaagg gctgctctta atgctgcatc
    13861 tgttttatag tctaaagcgg agaaactatc atcgaataca tagatctctg ctggtttgat
    13921 caacgctcga gcaatacata tccgctgttt ctggccccct gaataattag aaccgccttg
    13981 agcaacaaag ctgtccaatc cgtcaggaag ggtgtccact agttcttcta attgagcgat
    14041 cttgatcgct ttagacaact cttctgttga ggcatattca tttcccatct gcaagttgct
    14101 taaaattgtc ccgctgaata agaaagcctt ctgagggaca taactgatgt gggagcggat
    14161 ctgttcttga gtcatttgag taagaggaat accaccaagg gttacttttc ctatcgtagg
    14221 gtcactaagt ctcaagagaa gtttcgccaa actacttttt ccagatccgg tcccgccaac
    14281 gatcgctgtt gtttttcctt taggtattgt gaaatcaata tcttctagaa ctggatcaga
    14341 gatttcagta tatttaaatg tgacatgttc aaaagcagct gcatttgtct gatcaaaggt
    14401 tggttcagct actgttttga tcgtctcttc ctcaatttcg atctctgctt gcagtatttc
    14461 ttctatccgt cgtaatgagg caaacgattt agggataatg accaatacca tagctgcgat
    14521 catcaagtaa gcaagtgtca gcatagagta ttcgactaca gcagtcacga caccgatttg
    14581 taatgctcct tctgctacaa gatttccgcc aaaccacaaa atagaagcat aaacgattcc
    14641 catgagcaaa aaagcaattg gagtgaccca agcaaatatt cggttcactt tgatcatttg
    14701 attagcatag tcagcaaagc tcttattcgt tcgcttttct tcatatgatt gattatcaaa
    14761 ggcacggatc atattaatcc cagtaaagaa ttctctcaat gtgatggtga tccgatccat
    14821 ttttggctgg atactcaaag ataaaggtgc ccctcgtttc atcagtcgat aaacaatcca
    14881 tacataaaaa acaacagaga tcaatgtaac ccatgttagt aatagagaat aacgaaacat
    14941 catgagaatg gtgaaaacgc aaataaccgg tgctggtaag atcatttggc aaaataaaac
    15001 aatcatttgc tggacattat cgacatcatt cgtcattctt gtcaaaagag atccagaact
    15061 aaaacgactg atatccttca ttgacagctg ttggaattta cggaaatatt tctcccgcgt
    15121 ttggaatcca aacctagcgg aagccaatgc tgaaaagtaa ctagaggcaa ctgcagcgat
    15181 tgttcctaga aacgctacaa gcagcatttc aactccaatt ttttttataa ctgaggcttc
    15241 gccacttgct atacctgtgt cgatcagctt tgctactaat tttggtacac ctaatgtacc
    15301 ggcgatttct aaacagatca tcacaaaggt aaggagagcg agtcctttgt ttttctgtaa
    15361 gaatgttttt aagatagaca tattattcct ccttttttcc tgatactttt cgattctata
    15421 ccttcaagta acttgaaggt caatagaaaa aagaataaac tgaaaaagag gtgagaaaaa
    15481 atgaaaaaaa tgtatacgat tggccaagta tcggaactat ttgatttgcc aaaatcaacg
    15541 atccgctatt gggatgaaca aggattaatc cgttcaagta gacaagaaga gaatgattat
    15601 cgattgtttg atatcgatga tatttttatg ctttatgata tcgtttttta tagaaaatta
    15661 gatataccga tcaaacagat gaagaattta tatggaaaaa cattgacgga gttatatgag
    15721 acacttgatg aaacagaaag aagaatccat aaagaactgg tggtgatgaa acaaaaacaa
    15781 aaagagataa gggagcaaaa aaaacagttg aaattgatga tcgatacaaa cgaggaagaa
    15841 tttcctgtag aagagatccc ttttgactgt atgatctcaa cggagtttga agatattgtg
    15901 gaaatcaaaa aatttctacc taactattca agctttggga tgatgagtat gccagcttcc
    15961 agttcacaga cgatgtatgg attttttatc gaccctagtg aagtacattt atttactcaa
    16021 gacgtgattt gggagaaaaa agatacggct gtttatcgtc gattcttatt gaaatccgag
    16081 atgaaccatg cggaacgtaa taatatttta gaaatcagag aaagaatgta tgaaaaagga
    16141 tggaaaacag gagaagtgat cggccagtat ctattgacga atacggatga aaacaatatc
    16201 agaactgaat attatcatgc atggatagaa atgaaaaaat aggaaatatg tgtttaagat
    16261 aactcgaaaa tgatggttgt tttagttata aagaaaaatt ttctggttcg attacgggtt
    16321 taatgtgggg gattattagt cgtggataga tagaatttta gttttttaga ggaaatatgt
    16381 ctttaaaaat ggaatggttg caattcttag cgatatatac gatgtaattg tcctcttaaa
    16441 aaagtatgga aaaataattg tattgggtgt tatgtataat tcatggtgaa ctgagactaa
    16501 tgagaagaaa aattcatatt gttatgagtg agattgtatc agatgagaat gataaattaa
    16561 ataggtgcat aaaagcatta cacttaaaat acattgttgc ttacattatt acaatatgtt
    16621 aataaggcaa aaataaatag atggattgaa taaacatatt ttttaatcaa atagtcccta
    16681 ctgtgacttt cctcagctat cggtgatact accgatagca tttttttgct tataaaataa
    16741 ttataagtat ttagagattt ttcttctaac attcttcaaa caagaaaaat cacaaaggtt
    16801 attgttattt ttatatattc tttttaagaa tgattgtttt tttatagaaa ttttaaaaaa
    16861 taaaacacgt tattgatgga tagttagttt gttatttggt aatattatag aagttgactg
    16921 gtagtgattt atttttcgtc gttttataaa attgttattt tttggaaaaa ttcacaaata
    16981 tgtaacgaat gtttttctgg aatttgctgt gttaaaaagc agtatgacta atataaatac
    17041 tattgtcatt tatgcaattt tttatggata gtaattttag aggggaatca atgaaggaag
    17101 aatagaaggg aaaaatagtt gcaaatacgc cagttagcaa aaatagcaat acatcttcaa
    17161 gctttaaaat tcgagagaat catcttggta gtggaatcaa ctacaacaaa aggattaaac
    17221 caattaatag atagaattac caatggaaaa atagaaaagg tagttattct atacaaaagt
    17281 tgtttaattc gcttcggtta tgaacttagt gaaacccttt gcaatatatg gtacagaata
    17341 tagaaattat tgacattaca gaaaatgatg aagatttaat acagattgtc actatttgta
    17401 gatacctttt acaaggaaac cgagccaata aagctaagaa gatgattaag gagttgatgg
    17461 aagacgatac aagttaagaa agtgaaacta cgcccaaata aagagcaaaa agagtaattg
    17521 gacgtggaaa atgcaagaaa tgaaccatcg attcggtgga cagtttatta gtaatgacgg
    17581 tttgagaaca tccatgacga gaatgaagga acatcctaaa tgcgtttagt tgaaccaagt
    17641 gccaaataat atggccaaat aagcgatcag ttatcgatga atcccaagta ttctaaacta
    17701 tatattcatt ttgatgctaa gcattgatat atttttttgg gattgatgaa ggttaaccaa
    17761 tacagggact cactgatgaa attctagagt ctgaccttgg tatcaacgat ttagtgattg
    17821 tttctaatgg aaaagtataa aagtatataa gtacattaac aaaataacta ttagggttac
    17881 gctccagctt gtgcaaggca ctggcaggag gaatcgtgaa atcataatta tggttaagcc
    17941 acacggacaa ctaaaagtca tacaaagcta tcgaatcaat caacagaaga caatcggcaa
    18001 tcattagctt tgtttacagc ttacattgca acgcttgagt tgaaccgaaa atacgaggga
    18061 ggtaacgcag ccattttgag ttggggaatc tttttagaaa attatcgggt accttgttgt
    18121 ttagtcaaaa ttataagggg gagtaatagt gaggaacaaa tttcataaac tgttaagtat
    18181 gtttctagtt atgcttattg tgatcaatca atttgggaac ataacggttc tagctgagtc
    18241 tttagcagaa cagccagagc gttcgctgac gatcgaagtt gatcaagagg aggctgcttt
    18301 gggtgagcag ttccagttgc gagtcagagg aacaccagac ctgctaaatg cattggtcat
    18361 tgataagagt gaggatttga ttgaagggga agtgaagcaa ctcaatgatt acgaacgtct
    18421 gattacttat tcttcccagc attcaggtaa atacgaattg accgcaagaa gcggtgatat
    18481 tcaatcaaat acggtctcaa tagaaatcag tgtaccagaa gaaagcacaa gtgagtcttt
    18541 taatgtacca gaagaaagca caagtgaatc ttttaatata ccagaagaaa gcttaagtga
    18601 atcttttaat gaagccagat catctgagcc atctataacg caggaatctc agattgaaag
    18661 acaagaggag ctcttgaatt atgacatcgg catctctgct gagcgcccca ctttgccgag
    18721 caggcataca caaacgttaa agggatacat tcaaggaggc gctggcgata ttcttgaaga
    18781 atatggggag atggtcgtac gagttcctag agcatcgatt ggcttaattg agttcattag
    18841 agtggaggtc gctgatgatt cacctttaaa gccactggca ttttaccaag aaggcacaga
    18901 cgttgttttt ccatttacct ttgattatga aggattaaat gaagccgctc agtcgtatac
    18961 attcagtatc acgtttgaag ttgctttctt tcaatcaggc ggtactgagc caaataatga
    19021 ctttcaagtg agtcttactt atgctgagcg agcagcaaac acggaatttt caattacgcc
    19081 tattccttca tcaagaccca ttttttcgaa attatactat ggctctagag aaatcgacag
    19141 ccaaggaaat cttgggaatc tgctgattga cgggcaagac gtgaccaaca gtaataaatt
    19201 tattttgcag gtaaattatg aaggatcacg aaaagacggt gtagtggtta ccgatgtact
    19261 accgcaagga acaactttgg caccgaatcc gactacatgg agttccactg gaaatagaga
    19321 acctatttca ggtggggttc ggatcgcaga agtaacgaat ccaggtgaag caaacgttag
    19381 aactcattat gtttccgagg aatttgtgga caaagtatct tttaataagg aaacgaatac
    19441 acttgttgtg gactttggta atctagcaga gggtcaatcg tattttattg aatatgctct
    19501 tgaagtgacc gatgtcgact tgttcaatga aggaagacaa gaggatgggt tttctcggaa
    19561 tcgagcgagt atgacctaca ataatcaggt aatgagccga gactaccgct tgagaatccc
    19621 tgaaagtaat cttccggcaa tctctttaag aaaaagtgtg gacaaaaatg ttatcaatct
    19681 caatgaaaat gaactaacgt atactctgca tgtctcgatg ttttctggaa caagaaatac
    19741 gcttgttatt gaagatgttt tggaagaagg catggaattt gtgtcatttc tatcagatat
    19801 tccctctttc attgatgccg aagtcaataa tggaatagtt cgtttcagtg cgaataatac
    19861 gattggtggt ccgacaagtt ttaccctctc ttttaaggtc aatgtagagg cgtatgcggt
    19921 cggcgatcaa attttaaact ttgcaacgct tgtgaatagt gacaatcgat tgaatacgag
    19981 tactgtaaga acaaggaaaa tcgatgggcg tattaaagta attaaaactg atgagaatgg
    20041 ccaaaggctg gcaggagcaa aatttgaaat tttatcaagc gattcacaag tgatacaaga
    20101 aggtataacg gcagaaaacg gagaattttt aagtgaaccg cttcctgtag gtagctatca
    20161 agtccgagaa acagaagcac cggaagggta tcttttagat gcgacggtac atgatgtttt
    20221 tataaatgaa tactccatag aagaagtgac gttaaccatt gaaaacatcc ttgatacggg
    20281 tggtgttgaa ctaattaaac atgctgaagg cagtggtgaa gtccttcaag gagctgtttt
    20341 tgagttacag aaccgagaag gagaaacgct acaaaccggt
//
LOCUS       DAZBTF010000053        19629 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000053 DAZBTF010000000
VERSION     DAZBTF010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.053"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACHGNS_001942"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACHGNS_001942"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFN6937872.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="ACHGNS_001943"
     CDS             complement(1172..1867)
                     /locus_tag="ACHGNS_001943"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFN6937873.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="ACHGNS_001944"
     CDS             complement(1880..2596)
                     /locus_tag="ACHGNS_001944"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFN6937874.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="ACHGNS_001945"
     CDS             2926..3534
                     /locus_tag="ACHGNS_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFN6937875.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="ACHGNS_001946"
     CDS             3859..4182
                     /locus_tag="ACHGNS_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFN6937876.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="ACHGNS_001947"
     CDS             4364..5734
                     /locus_tag="ACHGNS_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFN6937877.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="ACHGNS_001948"
     CDS             complement(5864..7165)
                     /locus_tag="ACHGNS_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFN6937878.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9510)
                     /locus_tag="ACHGNS_001949"
     CDS             complement(7519..9510)
                     /locus_tag="ACHGNS_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFN6937879.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="ACHGNS_001950"
     CDS             complement(9654..9920)
                     /locus_tag="ACHGNS_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFN6937880.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ACHGNS_001951"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ACHGNS_001951"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFN6937881.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ACHGNS_001952"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ACHGNS_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFN6937882.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /gene="def"
                     /locus_tag="ACHGNS_001953"
     CDS             13264..13827
                     /gene="def"
                     /locus_tag="ACHGNS_001953"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFN6937883.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="ACHGNS_001954"
     CDS             complement(14080..14601)
                     /locus_tag="ACHGNS_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFN6937884.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="ACHGNS_001955"
     CDS             complement(14604..15446)
                     /locus_tag="ACHGNS_001955"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFN6937885.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="ACHGNS_001956"
     CDS             complement(15439..16164)
                     /locus_tag="ACHGNS_001956"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFN6937886.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="ACHGNS_001957"
     CDS             16340..16636
                     /locus_tag="ACHGNS_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937887.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16892..17066
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17204..18796
                     /locus_tag="ACHGNS_001958"
     CDS             17204..18796
                     /locus_tag="ACHGNS_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFN6937888.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="ACHGNS_001959"
     CDS             18809..19549
                     /locus_tag="ACHGNS_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFN6937889.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5617 a         4028 c         3724 g         6260 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagccattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaagtaaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgagatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttc
//
LOCUS       DAZBTF010000054        19235 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000054 DAZBTF010000000
VERSION     DAZBTF010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.054"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            153..1580
                     /locus_tag="ACHGNS_001960"
     CDS             153..1580
                     /locus_tag="ACHGNS_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6937890.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1672..2037)
                     /locus_tag="ACHGNS_001961"
     CDS             complement(1672..2037)
                     /locus_tag="ACHGNS_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFN6937891.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(2034..2399)
                     /locus_tag="ACHGNS_001962"
     CDS             complement(2034..2399)
                     /locus_tag="ACHGNS_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFN6937892.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2591..2839)
                     /locus_tag="ACHGNS_001963"
     CDS             complement(2591..2839)
                     /locus_tag="ACHGNS_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937893.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2838..2966
                     /locus_tag="ACHGNS_001964"
     CDS             2838..2966
                     /locus_tag="ACHGNS_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFN6937894.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            3023..4717
                     /locus_tag="ACHGNS_001965"
     CDS             3023..4717
                     /locus_tag="ACHGNS_001965"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFN6937895.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4817..4990
                     /locus_tag="ACHGNS_001966"
     CDS             4817..4990
                     /locus_tag="ACHGNS_001966"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937896.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5368..6501
                     /locus_tag="ACHGNS_001967"
     CDS             5368..6501
                     /locus_tag="ACHGNS_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFN6937897.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6532..6852
                     /locus_tag="ACHGNS_001968"
     CDS             6532..6852
                     /locus_tag="ACHGNS_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFN6937898.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6881..7345)
                     /locus_tag="ACHGNS_001969"
     CDS             complement(6881..7345)
                     /locus_tag="ACHGNS_001969"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6937899.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7477..8730)
                     /locus_tag="ACHGNS_001970"
     CDS             complement(7477..8730)
                     /locus_tag="ACHGNS_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFN6937900.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8759..9076)
                     /locus_tag="ACHGNS_001971"
     CDS             complement(8759..9076)
                     /locus_tag="ACHGNS_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFN6937901.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9102..9422)
                     /locus_tag="ACHGNS_001972"
     CDS             complement(9102..9422)
                     /locus_tag="ACHGNS_001972"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN6937902.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9886..11301)
                     /locus_tag="ACHGNS_001973"
     CDS             complement(9886..11301)
                     /locus_tag="ACHGNS_001973"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN6937903.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11318..12634)
                     /locus_tag="ACHGNS_001974"
     CDS             complement(11318..12634)
                     /locus_tag="ACHGNS_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN6937904.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12784..13482)
                     /locus_tag="ACHGNS_001975"
     CDS             complement(12784..13482)
                     /locus_tag="ACHGNS_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFN6937905.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13771..14760)
                     /locus_tag="ACHGNS_001976"
     CDS             complement(13771..14760)
                     /locus_tag="ACHGNS_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFN6937906.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14840..15673)
                     /gene="msrA"
                     /locus_tag="ACHGNS_001977"
     CDS             complement(14840..15673)
                     /gene="msrA"
                     /locus_tag="ACHGNS_001977"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFN6937907.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15848..17482)
                     /locus_tag="ACHGNS_001978"
     CDS             complement(15848..17482)
                     /locus_tag="ACHGNS_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFN6937908.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(17469..>19235)
                     /locus_tag="ACHGNS_001979"
     CDS             complement(17469..>19235)
                     /locus_tag="ACHGNS_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937909.1"
                     /translation="NLTQNLLKKDNSSILKIELKKENNKSMKISKKAFLLVVLILLST
                     LYSVNFMRNAQEIYTTGDLSFHLSRIKGLSSIFEGPINYTTFNNYGDGLNYFYPFLTI
                     IPAVVFYGISNNLILSYVLYIWLLNICTILISFYYGEKFFKKIDAAFLFSCLYTFATY
                     RTVDIYYRSAIAEAIALTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLL
                     CSALIACMIIVRLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIH
                     SPFKTDLQLEALSISDSIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYV
                     GVVSTWVCVTNLFPWFLFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYS
                     WLIIGATVVFLLVTSIAAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTE
                     AIKERPHLEKHEVKVGESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVN
                     GKVVNDSYKKHGLVNVDVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFA
                     PNKLKDSRYVTKKEINKKNENK"
BASE COUNT         6109 a         3759 c         3194 g         6173 t
ORIGIN      
        1 tagaattatc tgttttccaa attttataac ataaaaaacc ttttttaacg gcatttatat
       61 gctttttcag aaattaactg atgatttcaa aaagaaaaca tgtcataata tttcccatat
      121 tgtgcaaaaa aatacaaggc ggaggctcta ctatgattga agaatatatt gagaaaaata
      181 ttttaagaca gctgttttta tgtgggcaat tctatgtaaa taaagaagtc aatctagaaa
      241 agctctccaa tctcctgcat gtttgcaaaa ccactttact caatgatatc aataacataa
      301 aaaaagaatt tgaagaacag atcgcttata cacatcggga aaaggaccgt tacactttat
      361 atttttcaga acatatacct cgatgcaaga tcatgcagca attgtctcaa aattcgttgt
      421 ttttaaaaac ttgtctgctt tatttggaag aagatgaacc tgattatctt caattaactg
      481 aatgtgaatt catctctgtc agcaaagctt attcattaaa aaaacaagta ttagcttatt
      541 ttaatgactg cgggatcgaa atcgaccgct actcccctcg attcactgaa atggaaagac
      601 gtctgctgct tcttaatgtg tcgtatcgtt taggcggttt taattcttgg gagctacctg
      661 aatcgttttt tgaacgtgcc gatcgtttca ttgaatccgt aacggaaaat agtggcagat
      721 tttatgataa agaaaataaa gaaatcttat cgattggttt tgctatcagt ttcctacgcc
      781 agcaagtgtg tgctgtgaca atagattcaa aatttatcga agaaatcaaa aaacgtccta
      841 tttacaatta tgtcgaatca gcatgggaaa acactgattt ccaaacctat tataaaaaag
      901 aagaattcgc ctttattttg acactattca atctttgtaa ttatggtttc cattcttatc
      961 agttgattgc ggaagacttt caacagctac accaagtgtt tattgacaat actcccgaaa
     1021 taaaagaact agttgctacc tttgaatctc acttcaatca agaattattc ggaaaccaac
     1081 cgttcgaacg tgcgctgatt catctgatgc gttcggcttg ggataactat caattattca
     1141 tgccagaaaa attttatctt ttaaatgagg aacagacaaa tttatataaa gaagtccaga
     1201 caattttttc cagctggtcc agccagttac cttatgatct acggttgaat cctaattgca
     1261 tgcgggcttt tgtgatcgaa ttgagcggaa ttctccgtct gacaaaagaa catctgacta
     1321 tctatatcgt cacaaattcg gatgttcatt atctgattta tcgggaagcc ttggaagctg
     1381 taactacttt cgattttcaa gttgcaccaa caatttattc aagtatatct gatataaaga
     1441 agtatgcgca gcagtcctca aaccgggtat tatgtgaacg tacattatat acaccagatg
     1501 cggtccaata tgaaaatatt attcctattt cgatcaatac gatcgatcga gctatcattt
     1561 cagctgtgca aaacaaataa ggggctgtga caaaaatcat gtcacagcct cttatttgtt
     1621 gaagatgccc atttctgtaa caatctcttg atacacgtca ggctcacaac ctcacttatt
     1681 ttggacatct acgggtccga tctctttatt taaaaaataa gaaatgatgg ttcggataac
     1741 taccaacgcc gctagttgga aaatatcctg caaggtaggt ttgataatcg attcgatgat
     1801 cccagctgaa acaagaattt ccaaacttag aagtatatag cctcccaaca tctttttaat
     1861 agcattgttc tcgctgactc ggtcgctttg cacttttgta tggacaaata aatatttcaa
     1921 aaaatccttg attgatagac aaacacccca aaccaataaa cagatagcaa agatatcaag
     1981 tcccagtaca agtttttcga aatatggaag aagaaactgc atcaactctg tcgtcatcct
     2041 acctctcctc ttctttgttt ttctttgtct tctatttcct gattcaagaa ataagaaatg
     2101 actgttcgaa tgataataat aagtgcaagc ttcaaaatat ctgtccaagt cggcttgata
     2161 actgactcga tgatatcagc aacgactagt acttccaagc ttaacaaaat ataacttgct
     2221 agcagttttc ttgtatctgc attacgagta ttacgttctg catagttttt cttgacatga
     2281 gaccaatcaa aaagtttgaa tagaacaaga acgatcccga caattaagat accgatagaa
     2341 caaatattta aaatagcaat gatccattct agaaattcaa ttaaatttga gcgaaacata
     2401 tacgctacct actttctttt aatttgttcc tattctttta ttgtaaaaga aagttacctt
     2461 gttcgtctat ttaaaagttg ttctaatgat aacggttcgc ttaaattttt ctttttcact
     2521 ctcccatttt tctccctcca tccataaatc gtggaagtat ccaaaacaac gtagcaagtt
     2581 aaaataaggc ttagtccgtc atcagctttt caaagaagca ctgtctcggt gctttttttg
     2641 tacccaaatt tctaaaaata gaaccataca cctcctcaaa tcttttcttc ttacctaccc
     2701 tatttttatt tacaggaaaa tcagacactt cttctttttt gtcaaaattt cctttttctt
     2761 ctcgtcctat catttggcat tctaataaaa acgaactatc cttggatata gaaagagaaa
     2821 acaaggaggc gtttttcatg agtaaaaaat taatcaaagt cggcatcggt ttaggtttgc
     2881 ttgcccttgg cgctgcatat ctaggaaaaa agacaggact attcgaagat gacagtcatt
     2941 tatatgatga attcgaatca atctagcatc ctatttagtt gaatcactac ggacatactt
     3001 ggaagacatt ggaggaatca caatgagaaa aagtaaagca ataatgattg gtgccggtat
     3061 tgccaatatg gcagcggctg tttatttgat tcaagaaggc aaatggaaag gggatcagat
     3121 caccttttat tcattggatg atcacggttc aaatgatggt gcaccgaccg acactgtagt
     3181 ggatgaatat tggaataaaa accatccatt agaaaaccaa aaaggctata ttgctcgagg
     3241 cggtcgcatg cttaattacc gtacctatgt agatttgatg gatctgttaa gtcgaatacc
     3301 ttctgttact gagccaggga tgactgctga agaagatacg agagattttg atgcaaaaca
     3361 ccgaaccttt gacaaagcac gtttgatgga aggtggcaaa ggaatcattg atggcggaaa
     3421 actgggattc aataataaag accgtattct tctgactaaa ctgattgcta tgcctgattc
     3481 agaagaagaa aaactagaca atatcactat cgaagaatat ttcaaagatg atccacactt
     3541 ttttgaaaca aacttctggt ttatgtggga aacaacattt gctttcagaa cacgtagttc
     3601 tgctcaagaa ttgcgtcgct atatgcatca aatgatttat gaattcactc aaatcgaaca
     3661 tctagtgggc gtcaatcgaa cacgttacaa tcaatacgaa agtattatgc ttcctttgat
     3721 cgagtacttg aaagaacaag gatgcaaaat cattttgaac cgccgtgtca ctgactggga
     3781 attcaaagaa acaccgatgc aagatgaaat cactgtgact ggtttgaaaa ttaccaatac
     3841 actgactcaa gaagaagaat acgttgtggt cgatgaagat acagcagtta ttttcactaa
     3901 tgggtccatt acagattcag ctactttagg agattacgaa acaccagctg tagaaaatat
     3961 ggattatggt gcagcttcta gtctctggaa aaaagccagt gaacgtttct acaacttggg
     4021 aaatcctgac aaattctttg ctgatcgaga tgcaagcgaa tgggtcagct ttaccctgac
     4081 gacgaaaaac catttgttgt tgaatgaaat cacccgtatc acgactcaag aaccaggaaa
     4141 tgccttgaat tcatttattt caaccacacc gatcacgccg ttaggccaaa aagatgtcaa
     4201 tatgtctatt gtcgtgcatc atcaaccgca ttttacgtct cagaaaccaa atgaaacagt
     4261 tatttggggt tatttccttt atccaagacg acgtggcgaa tttgtggata aacagtatat
     4321 taaaatgaat ggaaaagaaa tgctagaaga actgattgga caactttcaa aagtagatcc
     4381 tggaccagtg aatattcgtg aaaaagaaac agaaattttc gacagcgtaa taaataatat
     4441 ccctgtctat atgccttatg cttccgcgtt gttcaataat cgtgccaaaa gcgatcgacc
     4501 aaaagtcatt cctaaacatt cgaccaatct ggcctttaca ggagaatttg tcgagcagcc
     4561 ttatcagatg atctttactg aacaaagtgc tgtacgttct ggagaaatcg ctgctttcca
     4621 ttttgcgggt attcctatgt cccgtcttgt caaaacaccg cgttacgaca aagatattcc
     4681 tactctagct cgtgccgcaa aaaaaatgtt tgaataatca agaggttgtg aaaaagctgc
     4741 cctgcatcaa gtaataagaa caaccaggct aaaacagctt cttatgtttt tgatttggtt
     4801 ggacttatta ccgcagatgc aagcttttga acaccgttta ccaagaggtt atgaaaaagc
     4861 tgccctgcat caagtaataa gagcagccaa gctaaaacag cttcttatgt ttttgacttg
     4921 gttagactta ttaccgcaga tgcaagcttt tgaacaccgt ttaccaagag gttatgaaaa
     4981 aggcgtttag tttcgagtat gaagaaaaat tttgtgaaaa tggttcctca cgtttttgca
     5041 taaatttgac ttaataccga gaaactgatc ccttctgtca taacctcttt ttatttatgt
     5101 ctgtttcttt ttgaaaacaa ctattttttc aacgtttact ctcttgccaa tccaacatca
     5161 tctaacggat acaactattt caatgattac cattacacta aaaaatcgga aacacacctc
     5221 tccgaacaca aatacataag actatagaat gtttcattta agaaaaaaag cgtttcatta
     5281 tcaaaaaggt aagatctcct ttaaaatgaa attatctgta gtactaataa tgcagacaat
     5341 tttatgaatc tgaaaggagc atctccaatg aaagcagtca cttggcaagg aaaacagaaa
     5401 atggaaatca gaaacgtaga tgatccaaaa atattagaac caacagatgc aatcatccgt
     5461 atcacagcaa cagccatttg cggctccgat ctgcatcttt accatcatgg tgaagcagtt
     5521 ttggaaaaag ggtatgttgt aggacacgaa ccaatgggaa tcgttgaaga agtgggatct
     5581 caagttcaaa cattgaaaaa agggcaacgt gtggttatcc cttttaatat tagttgtggt
     5641 cattgtcatt tctgcctgaa tcacatggaa agccaatgtg atgaatcaaa tccagatcag
     5701 ttatatggcg ggcttttcgg atttggcaag ttgaacggta atcattgggg tggtcaagcg
     5761 gaatatctaa gagttcctta tgcagactct acttcatttg ttgtaccaga taatgatctt
     5821 cctgatgaaa aagttttatt tctatcagat attttgccca cagcttattg gagcgttgaa
     5881 aacgcaggag taaaaaaagg cgatacagtt gtgattctag gttctggtcc agtcggtcta
     5941 ttcgctcaaa aattcgctgt tatggctgga gcagagcgcg taatcgcagt agatccagta
     6001 cagcaccgtc tagataaagc ggctaattac aatggggttg aaacttattt cttagaagac
     6061 acggcacaag ctggaacaga attgtatgaa ctggctaaag gtggcgcaga tgtcattatc
     6121 gattgtgtag gaatggacgg attagagcct gtcaaagaaa aagcgaaaaa tttagtaagc
     6181 ttacaaagcg gcacaatctc tccattacag atggcatcgc aagctgtcaa aaaatttgga
     6241 acagtccaag taactggtgt ttatatgact ccagcttctt cttacccgct gcaagaattt
     6301 tttatgcggg atatcgctgt caagcatgga caggcaccaa ttatccatct aatgccaaaa
     6361 atttacgcaa tgattgcaga aggactattc gatcctacac aaatcattac tcattcgctg
     6421 ccactatcag aagcagcaca agcatatgag atctttgaca aaaaagaaga taaaaatata
     6481 aaagttgttt tgaaaccttg aattcagtaa aaaatacagg tgattttctt tatgcccaaa
     6541 atcaaacgaa atacccatga gatcgaaata ggaaaagaag aagatatcga aatacgagga
     6601 cgtcgcttcc gcttctattt ccaaaatcgt tacacattga tctcactggc tgtcgattta
     6661 ttaaccggga ttttctatat ttttggaagc cttgcttcct taactaaaat ccccgatcga
     6721 tatggcacct atttttattt ggctggtgct attttcttga cgattcgtcc tattctcaga
     6781 atcgtaagga acatttttat ttacgacgaa aaaaaagcaa tgaagagaac aagcaaaaaa
     6841 aaagaggcat gagaattttt caaatctcat gcctcttttt tcaccctatc gtatcacttt
     6901 gatacagacg agcggtcgtc ccactttctt ccttgaccat tgtcaaaaat gtccactcgt
     6961 atcgttcata aaacgtggta tgatctgtaa ttaagtaaag tttttcgtac ccaagttgat
     7021 gcatttcttt tctgatttcc gctaatatcc gcttggctat cccctgctta cgcttttccg
     7081 gttcaacaaa cagggcacaa agatttggtg tcagatcagg gcggttatgg aagtcgtttt
     7141 caatgacgcc cgctcctccg acgatggttt ctttttcatc taagaccaca taccattgag
     7201 gaatactttc cttattagcg attccagtac gaatactttc ctcatacaca acagcaggaa
     7261 ctccccattt tgatgaaaac caattcgcgc tttgttttac taattctggg tgttctctta
     7321 atttgatcca gttatatgta accaatgttt aaacctccga ctaaaataaa acgtttacaa
     7381 aaattataca agtctttatg aaaattagca atgacaacaa tagtgatgta aaaaaaaaaa
     7441 cggaaaacaa tcacttgttc tccgtttttt gattaatcaa gcatctaaag cttcttttct
     7501 tctttcttgg atcgtagcta ccttgacgaa cggtagatag atcaaaatag aaagaaccaa
     7561 attgacagcg gctagtacac cacctgcaat actttttgtt gccaatacac cgccaataat
     7621 tggtggagtt acccatggag gcataacggt agctgctggc actaaacctg tagaagtcgc
     7681 aaagtaagca acactaacta agaccattgg tgtcaaaata aatggaatga acatgatcgg
     7741 gttcaacacg ataggtaacc caaacatcgt tggttcatta atattgaaga cacctggcgc
     7801 aatcgacaag tttgttacaa ccatatatgg tttgttttta cgtcctacta aataaatagc
     7861 taacagtaaa cctaaagttg cacctgttcc tcctaagttg acaaagctgt caaagaaagg
     7921 tttgttcacg ataaacggtg cgctatgtcc agctgcgatt gcattaacgt ttgcttcaat
     7981 ggctggcgca ttgattgttt gcatcaaagg atcgaccata ttcgctccgt gtaaaccaaa
     8041 gaaccataag aatggtgtga taaatgcaag cagtaaggca gatggataag agtttgcaag
     8101 tcccataaat ggttcttgga ccactgtata gaaagaacct acgatatcat caacaccgaa
     8161 tgcagcaaaa attgcagcaa ctaaagcaaa cgcagagata gtgatcattg ctggtagtaa
     8221 tgaagcaaat gcttttgcaa ctgccggtgg cacaccatca ggcattttga tgatcaattt
     8281 ttcatttccg cttaaacgta caaagatttc tgttgaaata atcccaacaa taatagcaat
     8341 aaacaaaccg tttgagctca tacctaatgc tccgcctaaa gcgaagaaag agcctaaaga
     8401 aatcacacct gcggaaacac cgtcttttcg gtagccgtta gctaaatgat aagcaagcaa
     8461 gaaagcaatc aagacagaaa gaatggcaaa tgtaccgttc caagcagcag ccccgaatcc
     8521 tttccatgat tcaccaccaa aaattgagtt catcaattct tggaacccgg gaatcggtaa
     8581 gttattgatc attactgcta atgctcctaa gatcattaac ggcatactca ccataaatga
     8641 gtcccgaatc gcaactaaat gccgttgtgc accaattttt gatgctacag gtataaaatg
     8701 tttctccata aatgcaacaa atttatccat aaatcttccc tcttttcttt actgtagatt
     8761 acgcgttcaa tcgacgatac agatcaatga tctcttttgc taaatctgta aaagctatag
     8821 atgtcatcag atgatcttga ctgtggaccg tcaaaagcgt gacttcaacg tgatttcctt
     8881 gtgcttcttg tgtcagcatc tctgtttgag agtgatgtgc ttggactaaa gattcctctg
     8941 catctttgat tttttcatct gctaaagtga aatcaccttt tttcgcagca gcaatcgctt
     9001 ccattgcatc gctttttgca tttccgccaa acatgatcag acccatgatg gcttctagat
     9061 ttttttgatc ttccatttat tgtatcctcc gataattttt attatttgtt tattagtttt
     9121 tctgcttggt cgagtacttt ttcaccattc atcatgccat agtcagccat attgattaca
     9181 tctaaaggaa tgcctttagg tgctagcttt tcagcaaatt gattttgcat aaagcgtact
     9241 tgcggtccta aaagtaaaac gtctacattt ttagattcta gttgattatc cgcatctgaa
     9301 gcagatacag caaagatgtc tgcttccatt ccttttgctt ctgctgcctt ttgcattttt
     9361 gtgactagga gacttgtact cattccagct gaacatacaa gcatgattgt tttcttttcc
     9421 attttaaaca cgttcctttc tatacttaca cttgtatata agcaagtagc gtgccaactt
     9481 taaaagcgct ttaaaaagtt gatatcatca catttaaggt ctataccaag ttgcatacac
     9541 actaaaaaag aaagcgcaca ctaaacacac aaattaattt ttattacctt ttttctttta
     9601 acgtgcacaa aaaaagttgt gacacaaaac ttgtcacaac ttttttcaaa taaacggtat
     9661 tcaaaagctt gcatctgcgg taataagtcc aaccaagtta aaaacataag atgttgtttt
     9721 tactttgcta ttcttattac ttgatgcagg acagcttttt cacaacctct caataaacgg
     9781 tgttcaatca gctgaccctg ggtattagct caaaacgctg gattcacaac ctcttttatt
     9841 tcaaataacc agtaaataca atgaacatct acaaaaaact tctatttatt tatagatttc
     9901 gtcaggcaaa tccaattctc tcttatcaat caaagtctga taccacgaag cagatttttt
     9961 caatgttctg cttcgttcat cgttcaattc gattcgaact aatccatatc gattcttaaa
    10021 tgcattcatt ggtgacacac agtcagtgaa tgcccataac atatagcctt cgcaatttgc
    10081 gccttcttct gttgctttga tcaagtaatg caaatgctgt ccgataaatt cgatacggta
    10141 atcatcttgc acgattcctt tctcatccat atactgttct tcatattctc ttcccatacc
    10201 gttttctgtt atcagccaag ggatattatg ataattttct tttagataca tggctatatc
    10261 atacatgatt ttcggataga tttcccatcc cctggaatta ttcattttct tcccaggtaa
    10321 atcaaagact tcataatatc gttcaggatg gaaaggtgtc tcttcattcc attgataacg
    10381 aggggcgcga acgcgtttag gataatattg attgattcca aggaaatcta ctgtatgatg
    10441 ctgaatcgtt tctaaatcct ctgttgcagg atcgaaataa atcgattgtt tttgacacaa
    10501 ctccaggaac tcttctgtgt agtatccttt taccatcgga tcaaaaaata ctcgattata
    10561 aaacaaatca tacatataag cagctttttg gtcttctgga gaattggatc gagcatagac
    10621 catttcagga tttaaaatac agccaatcct tccaccataa tttccgctgc ggtaaatctc
    10681 tactgcttta gccgttgcta aaactttatg ataattccac atcatccact tttttgtgtt
    10741 ttgttcatgc ggccagcgaa cagcatccaa atagcaacgc gtttggggaa cgattggttc
    10801 gttgaatgta aaccagtttt ttaccctatc accgtaacga tcaaaggcaa tttgagcata
    10861 cttagcataa aaatcaacta cttttcttga agaccagcca tcatattttt ccaaaagaaa
    10921 agctgggact tcataatgtt ccaaacaaat catcggttct acgtctgctt cgatcaaaga
    10981 gtcgatcatt tcacttatat gctgagcata ttcttcatct actatacagt tctcataatc
    11041 agtaaaaaaa cgagcccaat tgatagaagt ccgataatgt gtcagatgga tggatttcat
    11101 cagatccaca tcctgctggt attgttccat aaaattagtt gctacggctg ggccataccc
    11161 attatgccaa acaaaatgtt catttttata ccagctatct ataaacgaat cctgtccttc
    11221 tttttttcct ttccaacctt ccgtttgcca agcagaagat gcagcaccaa gaataaaatt
    11281 ttttggaata gtaattttca tgatttcctc ctgtttttta gatcccatca ttttccacac
    11341 gatttgctac ttttacaaat ggcagataga tcaaaataga aacaataata caaacaagtt
    11401 gcgtaataac tgcaccgata tttcctgctg ttgccagata agcattgacg atcggcggca
    11461 ttggccaagg gaccattaca actgctttcg ccgcaaatcc taaagcggtt gcaaaataac
    11521 caatcgttcc agtgataaga ggcgttaaga taaacggaat agctaaaatt ggattcagca
    11581 tgatcggcat accaaaaata actggttcat tgatgttgaa gatcccagga gcaagggata
    11641 acttggtgat ttgtttcata tcttcccgtt ttccaactaa caatactgca atcagtaaac
    11701 cgatcgtgac tccagacccg cccatactca tatacatatc ccaaaatggc atagtaataa
    11761 tgttcggtac ttcttttcca gcttcaaaag cttccgtatt tacagcaatt gccgctaaaa
    11821 gcaacggttc ccgtatcggc ttcaccattt gatttccatg gataccaatc acccaaaaga
    11881 cttgcgcaat aaacatcaat agcaaaatac ccggtaaccc ttgtacgaca ccttctaatg
    11941 ggcgttgtac gacattgtaa atgatatcat aaaggtacat tcctgtaagt tctttgatga
    12001 taaatcccgc agccgcgata cccgatacag tcagaatcgt cgggaataaa gcagaaaaac
    12061 tagcagaaac attcgttggc acggtatcag gcattctcag ctgtagtttc ttctgtttcc
    12121 tcaaccagca ataaagttca atagaaagga tggagaccaa cattcccaaa aataaccctt
    12181 tcgtatctgt atattgttta gccaatacgt tttctactat aacagtctga ttttcattca
    12241 ctaattcata aacagttgga ttaacagata aataagacat cactgcgatg ataccaggga
    12301 atacaccatc cactttattc agcttggcta attcgatccc tatcagaaaa acagcataga
    12361 tggtcaagaa acttagtgtg acatagctga tggcagatgc gataggcttt aattcttcta
    12421 aaaaacgcaa gaaacttatc tgtgctaagc cattttccga gtcaaagacc attgctgaaa
    12481 acaatgtgcc aaaagcaccc gtaataatta ccggaatcaa tgcagcaaat gcgtttttta
    12541 tcaccatgat ataccttaaa ttattgattc tcgttgccac gttccctaaa acattggcta
    12601 atttatccat aaaactcatt ccagatcccc tcattcctgc ttcttctcct tgacgtcatc
    12661 tctttttgtc tattagtcag cgcttacatt tcgatgaaca gaaactattt actcctcttt
    12721 tttaccaaat ataaaacaaa aattcatctg atgtatttat cataaaatac atcagatgaa
    12781 ctgtcaatta gtttcttttt ctcctattat tttctgtaat tctctccgat ttattgccat
    12841 atggacagtc attgcatcaa acgcagcaag tgcattccgc ctttcgatgg cctgaacgat
    12901 ctctgtatga atatcgatcg tctcgatttt cagatgctgg atatcataat ttttattaaa
    12961 caaagcaatt gattcattaa tgattgggat aatatgcagc aaggctacgt ttccgctagc
    13021 ttctgcaatc atcgtatgaa aatcgatgtc taattgaaca tgttcttgtg tgccttctcg
    13081 aaaagaagtt tcaatctttt gcgttaattt tttcatttgg acgatttgat tcttcgttgc
    13141 atattccgct gccaaacctg ccatcttagg ctccaataaa tatctcagtt caaataagtc
    13201 tcgtatcatc cgatcctgat cttcaatcaa agaaaaaccg agtgggtcat tggcaatccc
    13261 tcgcttatca ctgacaaatg tccccgctcc ttgccgaact tcaaggatat tcctggaagc
    13321 tagagcccgc acagcttctc gcaccgtgct tcgccccacc tctaaataat cggacaattc
    13381 atattcattc ggcagtttat ctcctacttg caactgcatt tcctttaaat atctaaggat
    13441 cttgtcagta gttacttcta ctagggtttt attttcttcc aaaccacata cctccttttc
    13501 atctgatgta ttttttcgta attattataa cacgtttgaa tcatctgatg attttttgta
    13561 tacaaaattg attaatccgt tcatagacat acaaacgaag aagatattta ccataaaaaa
    13621 gactaggata aaattcactc ctagccttcg atcattttta ttttgaacga caaaaaatat
    13681 gatgttcaat aacagacgat cttcattcga ttcttgatgt catcttttgt tttttttaat
    13741 atatggataa aaagtttgat tacatcgttt ctattctaga atttggtcga ttctcaattc
    13801 ttcttctggt gagaaatcta aattatccat cattcgaaca ctttcttgta actgacttaa
    13861 ccgacttgct ccgaccagta cacttgtaac agatttttgt cgcaaattcc atgcaagggc
    13921 catttgcgct aatgattgtc ctcgtgatac ggcgattgtt tgcagtgctt tgattttttc
    13981 cagagttgat ccaacttgtt cttctgaaag gaatggtatt tccttacgat gtgctctaga
    14041 atcttcagga ataccatgta gataacgatt tgtcagcaac ccttgagcaa gcggactgaa
    14101 cgtgattgcc cctagccctt cttcttctaa tactggagat agatcgtctt ctagtagtgc
    14161 acgagaaaac atattgtagc gcatctgatg aataatgaac ggtgcttcct ttctttttaa
    14221 aatttcagtc attttttttg tatcttctcc attatagttg gatataccaa tatataatgc
    14281 ttttccttgt cttacaagtt gcatcaaagc ttctgctgtt tcttccaaag gtgtatcagg
    14341 gtctgggcga tgatgataga agatgtcaac ataatctagc cccattcttt ggagactttg
    14401 gtcacaactt gaaatgatgg atttacgaga gccccattcc ccatatggcc cttcccacat
    14461 atgataacct gctttgctag aaataatcag ttcatcacgg taggcttgaa aatcttctct
    14521 aaaaatacgc ccaaagtttt tctctgcagc tccagcaggg cctccataat tattcgcaag
    14581 gtcgaaatgg gtaattccca tgtcgaatgc gcctcttaaa atctctcttt gattatggat
    14641 aggatcataa tcaccaaaat tttgccataa tccgagagat aaaatcggta atttcaaacc
    14701 tgaatgaccg gatttacgat attccatttt ttcataacgt gcactatctg caatatacaa
    14761 tttttctcct cctcatcaaa tctttttaaa acgctttctt ttttctattt ttagtttact
    14821 acattttaaa gaaaaggagt taatcaaaat gattttttcg tatatttttt tgccatttgt
    14881 tttttactcg ttgatagatc agaaattgat tacgtgcttg ctggattttt ttataacgtt
    14941 tcggttgttt tttgtaaaat tgctgatgat agttttctgc tggccaaaac gtaccggctg
    15001 gttcgatttt tgtgacgatt ggttggtgga aagttcctga actgtctagt ttctgttttg
    15061 attgttccgc taattctttt tgtcgttcga cttgataaaa aatcactgga cgatattgct
    15121 tccctctatc ttgaaattgt ccaaatgtat ctgtgggatc agtaatttgc caatagattg
    15181 tcagcaactc gctataactt atttctcttg tgtcataaat gatctccaca gcttccacat
    15241 gacctgtgta accaccactt acttgatcat aatttggttt ttcaacatgt ccgcccgtgt
    15301 atcctgacaa aacagatagg atcccttttt tcgattcaaa cggctcgacc atacaccaga
    15361 aacatccccc tgcaaatact gcacgttctt gatagtcagc atcttgcatg gcatgcttgg
    15421 aaaagtcata tttttgctcc ttatcaaatc cttccgtaat caataaataa aagtccatta
    15481 catcaggcgt cagattcatt cggatagcta atggacgtag tgttgcttct agttttgata
    15541 actgttcttt aacggatata ttttctctta tgtcattctt tgtctgaatc aatacttttc
    15601 gttcccaatc tcgtgtattc ggattcaaaa tcagattata aagtgtttga atgaatattt
    15661 gttcctcatc catagattct cctcctttcc gaagaagaca agctagtgac tttagctgta
    15721 atttatcata aagtgaggaa ataagcgatt acttcatccc cctacagatt tttcgtataa
    15781 aaaagagaga atccttatcg acagattctc ctttttaatc aatttatttt ataaatgaaa
    15841 agttaattta ccttttatgg aaaactattt taaaaaataa acataaatat gcagttaatg
    15901 taacaagata agaaataata gtcgttactg tataacttgt gcttacttta atatcagcaa
    15961 ctcctttgac tccgcttatt tgtaccaatc cattagcatt cacctgctcc tttacaggtt
    16021 gatgatttat ttctacattc gttcctaaat aagcatatac aggaagtgtg attacttggc
    16081 tcttctcatt agttactcta tatatgatag aactaccagt tgttttataa ggaaggttta
    16141 cccattgact gccaatcttt gcttcgtgtt ttattatatg ctccttattc tccaatgaag
    16201 cgataggaga ataatcatat aaaaaataat ccattccttt agtggtatac gcctctactg
    16261 tttctttgtt tacaacaata tgtcccggct gagaaattat tttttgattg aaattttttt
    16321 tactagaaac agttaaagtg attaataaca aaacagtcat aaataaataa aatagttctc
    16381 ttgtcttatt gtatcttcta tttttgaaaa atatcatcgt taatatcaga gaagagaata
    16441 ttatttgcaa gcttaagatc cgccaaggaa attgtaacaa tttggctggc gtattttgca
    16501 gaagatacca tggcaccaaa ctagttgtag ctagccaagt caaacacgta ccataataaa
    16561 tctttttttc tatgtttgtt aattttttaa ataaaatcag gggaatacat aaagataaca
    16621 gaccaactag tcccattgag taagtagtta agtcgttgtt catagcttca gtcagactat
    16681 cgaacacatt agatgcatga gcatataaat ttgtcacgct aggtttgttg attttttggt
    16741 agagcatttg ttcaaacatc ggataccaaa aagcactcgt cagaaccaat gtcattcctg
    16801 ccgcactaaa taatttttta aatatagcaa taaaatctgc attcttgaaa cctttagaaa
    16861 ccaaacgaat aaaaataaaa atgataatta atgctgtact cattaatgtt gatagtacat
    16921 ggctgtacac tagcaaactc atactcaatg ctagctttac agacggatac ttttcttctt
    16981 ttccagagat gatttgataa gcataaaata atacagggat aacaagtgtt attgcgatag
    17041 cttcggcaat tgcggaacga tagtagatgt ctactgtccg ataagcagag aaaatataga
    17101 gacaactaaa taaaaatgcc gctttttttt gttttaaaaa ccgctcgcca taatgataag
    17161 ctacgagtgt tgtacacaag ttcaataacc aaacgtaaat aatatagctt acaatcaaat
    17221 tttttgtcat ccaatataac attacagctg gaaaaaaagt cagaaaggga taaaaataat
    17281 ttacaccata tccagtataa ttgaatgtct cataattaat cggactggta aaaatagtcg
    17341 ataacccctt gattcgagat aagtgaaaag aaagatctcc agtggtataa atactctgtg
    17401 tatttttgaa aaagatgaaa gaatatatac tactaactat aattaatcca ctcaatatta
    17461 ctactttttt atttattttc attcttcttg tttatctcct ttttagtcac atatcgagaa
    17521 tctttcaact tattaggtgc aaatcgttta aaacttgaat acaccactaa acaataagtc
    17581 agaatactga tccataaagc catgagagca gttggagtat attttgttgt aatttctatc
    17641 ttattttctc cagcatttac atccacattc actaagccat gttttttata agagtcattt
    17701 acgactttac cgtttacttt tacctgtgta cctaaataag catatactgg tactgtcact
    17761 ttttgctttt tgttgcttgc cactgtgtaa gtaatgttat tatctgcagc tttatacttc
    17821 cctttttccc acgattctcc tacttttact tcatgttttt ctaagtgagg cctttctttt
    17881 atcgcttctg ttggggcgta atcgtataat cctcccatat ctgatgttgt ataacgtggg
    17941 agtgtttctt cattgataac aatatgaccg tgttttgatt ggataacttt tgcgtaattt
    18001 tcttttgctg ctatagacgt gacgagtaga aacaccacag tagcgccaat gattaaccat
    18061 gaatattttt tattcatagt ctttttcata aaaacaagcg ttatgatcat tgaagaaaaa
    18121 aagatttgga tacctaagat acgccaagga aactggagta agcttatcgg tgttttttga
    18181 aacaaaaacc atggaaataa attagttacg catacccatg tagaaactac gccaacatag
    18241 atgatttttt cttttttctc catttctttc agtatgaata aaggaaaaat caacgcaacc
    18301 aatcctaata accccatagt gtatgttgta atatcgttat ttaacgcttg aatgatacta
    18361 tctgaaatag aaagtgcttc tagctgcaaa tctgttttga agggactgtg tatttcttga
    18421 taattcatct gctgcaacat aggataccaa aaatagctag ttaatgcaca agtcaaaaaa
    18481 acagccttta ctaaattcac gaccacaaat tttatatcgt tttttatttg cttgcttaaa
    18541 aacaaacgaa ctataatcat acaagcaatc aatgcactac acaatagtgt agttaagaca
    18601 tgagtataca caagtagact tatactgaca gataaagcga tgactttttt ttctctattg
    18661 aatatcaatc cataagcata atataaaaca gctgggataa tcgtaagagc gattgcttct
    18721 gcaattgcgg atcgataata gatatcaact gttcgataag ttgcaaaagt atataaacaa
    18781 ctaaataaaa aagcagcatc tatcttttta aaaaactttt ctccataata aaaactaatc
    18841 agaatcgtac aaatatttaa taaccaaata tatagaacat aacttaatat taagttattt
    18901 gaaattccat aaaaaacgac tgcaggaatt attgtaagaa aaggataaaa atagttcagt
    18961 ccatctccat aattgttaaa tgttgtatag ttgattggcc cttcaaaaat agaagataac
    19021 cctttgattc gtgaaagatg aaaagataaa tcacctgttg tataaatttc ttgagcattt
    19081 ctcataaaat ttactgaata taaagtacta agtaagataa gaactactaa aagaaatgct
    19141 tttttactta ttttcataga tttgttgttc tcctttttta attctatttt cagtatacta
    19201 gaattgtctt tttttaataa gttttgcgtt aaatt
//
LOCUS       DAZBTF010000055        18504 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000055 DAZBTF010000000
VERSION     DAZBTF010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.055"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..746
                     /locus_tag="ACHGNS_001980"
     CDS             99..746
                     /locus_tag="ACHGNS_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFN6937910.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKLLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="ACHGNS_001981"
     CDS             1139..3784
                     /locus_tag="ACHGNS_001981"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFN6937911.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="ACHGNS_001982"
     CDS             4094..5407
                     /locus_tag="ACHGNS_001982"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFN6937912.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="ACHGNS_001983"
     CDS             5397..6050
                     /locus_tag="ACHGNS_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN6937913.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /gene="radC"
                     /locus_tag="ACHGNS_001984"
     CDS             6105..6797
                     /gene="radC"
                     /locus_tag="ACHGNS_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFN6937914.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="ACHGNS_001985"
     CDS             6947..7147
                     /locus_tag="ACHGNS_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN6937915.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7401..9080)
                     /locus_tag="ACHGNS_001986"
     CDS             complement(7401..9080)
                     /locus_tag="ACHGNS_001986"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFN6937916.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9082..9294)
                     /locus_tag="ACHGNS_001987"
     CDS             complement(9082..9294)
                     /locus_tag="ACHGNS_001987"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFN6937917.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9687..11417
                     /gene="efrA"
                     /locus_tag="ACHGNS_001988"
     CDS             9687..11417
                     /gene="efrA"
                     /locus_tag="ACHGNS_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFN6937918.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11414..13174
                     /gene="efrB"
                     /locus_tag="ACHGNS_001989"
     CDS             11414..13174
                     /gene="efrB"
                     /locus_tag="ACHGNS_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFN6937919.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13269..13466
                     /locus_tag="ACHGNS_001990"
     CDS             13269..13466
                     /locus_tag="ACHGNS_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937920.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13619..14104
                     /locus_tag="ACHGNS_001991"
     CDS             13619..14104
                     /locus_tag="ACHGNS_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFN6937921.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="ACHGNS_001992"
     CDS             complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="ACHGNS_001992"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFN6937922.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            14981..15508
                     /locus_tag="ACHGNS_001993"
     CDS             14981..15508
                     /locus_tag="ACHGNS_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937923.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            15599..16336
                     /locus_tag="ACHGNS_001994"
     CDS             15599..16336
                     /locus_tag="ACHGNS_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFN6937924.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16340..17257
                     /gene="miaA"
                     /locus_tag="ACHGNS_001995"
     CDS             16340..17257
                     /gene="miaA"
                     /locus_tag="ACHGNS_001995"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFN6937925.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17259..18488
                     /gene="hflX"
                     /locus_tag="ACHGNS_001996"
     CDS             17259..18488
                     /gene="hflX"
                     /locus_tag="ACHGNS_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFN6937926.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5983 a         3201 c         4022 g         5298 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ctaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag ttgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt atttttttgt
     7261 atgaaaaagt aaataaattt aagaggctat gaaagagcca ccttgtatca aggaataaga
     7321 acaaacaaaa atggcttctc atgtttttca tttgttttct cttatattca ggacaaggtg
     7381 ctgatttcat aacctcttat ttattcttca tctggtgtca ttaccattgg caaaatcatt
     7441 ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg cgccagtcat cgcttctttc
     7501 aatttatatt ctgaacagtc aggtccattc aaacattctc ttaaagcgtg gaataagatt
     7561 cgctgagctt cattgatcat ttctcctgat tctcgcatgt agacaaatcc acgagagaga
     7621 atatctggac cagccaagat ttcttttttc ttgatatcca ctgtcgcgac agctaataca
     7681 agaccttctt ctgaaagcag gtgacggtca cgtaaaacga cattaccaat atcaccgatc
     7741 ccgctaccat cgatatacac atctccagcc acaaaatgcc cagcaggacg cgcagaatcg
     7801 ctagttaacg ctaaaacatc gccattttcc atgatgaagc agttttctgc aggaacacct
     7861 acatcttgtg ctagagaagc atgtactttc aacatgcgga attctccgtg gacaggaata
     7921 aaatattttg gcttcatcaa acgaagcatt aatttttgtt cttcttgtcc accgtgaccg
     7981 gaagtatgga tgttattgat ttttccgtga atcacttctg caccagcttc agacagcaaa
     8041 ttgatcaaat gattgacact tgttgtatta ccaggaatag gtgaacttga aaagatcact
     8101 gtatcgtctg gctggatttg gatttgacgg tgagtaccat tcgcgatgcg gctcaacgcg
     8161 gccatcgctt ctccttgaga acctgtacat aggatcatcg tttcattggc aggcaagcga
     8221 ttgatttcgt gtgcatctac aaatgtgccg tctggaactt tgatatatcc taaacgttga
     8281 ccgttgacga tggcattttc catgcttcgt ccgaaaacag cgattttacg accagttgcc
     8341 acagcggcat ctgctgcttg ctgcaaacgg aagatgttag aagcaaagct ggcaaaaatg
     8401 atacgtcctt cgatttttga aaagatcttc aaaatagaat gcccaatcgt tttttctgat
     8461 ttagtaaatg ttggaatctc agcatttgta ctatcagata aaaggcacaa gactccttct
     8521 tccccgatac gagccatttt atgcaagttt gctggttcgc caacaggagt gaaatcgaat
     8581 ttgaagtctc cagtcgctac gatatttcca gaaggtgttt tcaccacgat ccctaatgca
     8641 tctgggatac tatgggtcgt tctgaaaaag ctgacagaag tctttctaaa acggatgatc
     8701 gtatcttcat tgatctcatg gagctctgca tctcgaagca agccatgctc atctaatttg
     8761 tttgtgatca acgctaatgc gagaggacca gcatagatag gaatatttgc ttgtttcagc
     8821 aagtagggta ttccgccgat gtggtcttcg tgcccgtgag taatcaccaa ggcttttact
     8881 ttgtgtaagt tttgtatgat gtagctgtag tcgggaatga catagtcaat tccaagtaaa
     8941 tcgtcttccg ggaatttgat cccggcatca atcaaaatga tttcatcttg gaattgtacc
     9001 ccatacgtat ttttcccgat ttcacctaat ccaccaatag caaagacgcc tgtctcgtta
     9061 tttttgatgg aaagtttcat attaaaactc cactaatgag aaatcagcat tttctttttc
     9121 gtactctaga tgacttcctt ctagaagttg aacgaattca atattgtatg gggtattttc
     9181 ttccacacgt tggcgagcgt ctacatcgct ttttgcctca atgtaaagag attttgtttc
     9241 ttcccgttta gggttgcgta cttttgtttc ttgataataa actttataga tcattgtttt
     9301 gtctccttta tctgcgtaaa gcagtgattt agctagttga tttttttaga caacacaaaa
     9361 cgaggggaca agccacctgg tttttcatat gctttgacta gaatactgga ttcgagtcaa
     9421 agagacaagc ctcgtttttg cttatctgtt cggatatttt ttcaaccgta ccttctgtat
     9481 aaaaaatata caacaattaa ttgttattct agcataaaac agcaaataag tatagaaatc
     9541 aactagattg aaagaaaaaa tcaatgttta tcatcccgct ataatcataa tgactttttt
     9601 atgagagaag tgttctatta ataggctcgt tgtgctatca tttttagtaa tgaaaatgta
     9661 acaaaagatt aaaaggagat gagaaagtga aactaatgtg gcattatacc atgcgttata
     9721 agaaattctt gtttttcaat ttcatatgcg tatttggttt tattttgatt gaattaggac
     9781 ttcctacgat tttagcacgt atgatcgatg taggaatccg caatgatgat tttgattatg
     9841 tgaagcaaca agggatattt atgatcgtta ttactgtaat cggtattatt ttgaatatct
     9901 tgttaggtta ttttggttcg aggattacga ctaatatcgt agcagatatt cgagatgatt
     9961 tgttcgaaaa gatccaaagt tattcgcatc aggaatacga aaatctaggc gtatcttcat
    10021 tgatcactcg tacaacaaac gatgcttatc agatcatgtt gttcctgcaa aatattttac
    10081 gtatcggttt tatggcacca atgatgttca ttgtcagttt gtacatggtc atgcgtacaa
    10141 gtgtgacact cagtttgtat gtagtaggtg cattaccttt actattattg gcagtcgtag
    10201 cgattgctaa ggtgtctgaa ccgttatcta aaaagcaaca aaaaaactta gataaaatca
    10261 acagtatctt gcgggaaaat ctttcaggat tgcgtgtgat tcgtgcattc gtcaatgaaa
    10321 agtttgaaga aaaacgtttt gctgtagtca atgagaatta tgcttctagt tcaaaaagct
    10381 tattccgttt gatggctgtt gcacaaccgg gtttttattt cttatttaat atcgtaatgg
    10441 tattgatcat ctggagcggg gcagtgcaaa tcgatgcagg cgagcttcgt gtaggagatt
    10501 tgattgcgtt tatcgagtat atattccatg ccttgttctc attcatgtta tttgctagtg
    10561 tgttcatgat gtatccaaga gcagctgttt cagctggacg tatccaagaa gccctagatg
    10621 ccaaaccttc gattgtagaa gatccaaatg gtatatctga tacaaaaact aaagggtata
    10681 tcgaatttca aaatgtaaca tttgcttatc caggccattc gcaagagcca gtgatacgaa
    10741 atgtcagttt tacagcctct ccaggagaga ccgttgcttt tattggaagt accggtagcg
    10801 gaaaatctac gttgatccaa ttgatccctc gcttttatga tgtgagtgaa ggaaagatct
    10861 tgatcgatgg agtagacgtg agagattatc ggctaagcaa gttgcgagat aagattgggt
    10921 ttattccgca aaaagcgctg ctatttactg gaacgattgc agataatttg cgatacggga
    10981 aagaagatgc tacgcaagaa gaaatggaac gtgcagctga gattgctcaa gcttcagaat
    11041 tcatttctcg aaaaccagat ggatttaatg aacatttatc agaaggtggt tccaattttt
    11101 caggtggaca aaaacaacga ttggcaatag ccagagccat catacgccgt ccggaaatct
    11161 atatttttga tgacagcttt tctgcattag attatcaaac ggatgcgaag ttacgtgctc
    11221 gtctgaaaaa agaaacgaca gaatctactg tattgatcgt agcacaacgt gtgggaacga
    11281 tcatgcatgc agataagatc atcgtattaa atgaaggaga agtcgtcggt atggggacgc
    11341 atcgggagtt gttggaaaaa tgcccgattt attatgatat tgccgcttct caattatcaa
    11401 aggaggaatt agcatgaaaa aaaccttttc ttccttaaaa cgtttagctc gatatatcag
    11461 accgtatcgt gagacttttc ttttagtgat cctatttaca gttctgacag tggctttcaa
    11521 cgtggctttg ccatatgtta caggacttcc tactacagaa atcagccgta atattgccaa
    11581 tggcgaatca atcaattttt cttatatatt caaatgtctc atttggatca cagttgttgg
    11641 aatagggtat tgtatttcgc aattactttc tggtgttttg atgacaaatg tcgttcaaag
    11701 tgccatgcat gatttgagaa gagacataga agaaaaaatc aatcgtcttc cggtttcata
    11761 tttcgataaa aaccagcaag gaaatatttt gtctcgtgtg acgaatgatg tagatgcagt
    11821 cagcggtgct ttacaacaag cttttattgg tatcgtaaat gcgatattgg gaattacgtt
    11881 ggctgcagca atgatgtttt atatccagcc gatgatggcg ctgatctcta tgatcatgat
    11941 tccaacatcg atctggatct caaaacgagt gatcaatgct tcgcagaaat attttcaaag
    12001 catgcagaat tctcttggtg agctaaacgg ctacgtccaa gaaaatatga caggttttag
    12061 tgtattgaaa gtatatggac gcgaaaaaga aacattggaa ggattcaaaa aagttaatca
    12121 cagcttgaaa tactttgggt ttcgtgcagc attcatttct ggcttgatga tgcctctagt
    12181 tcaattaact gcctatgcta cttatattgg tatggctgtt ctaggaagct tttatgttat
    12241 tactggggcg atcgttgtcg gtcaactaca agcattcatc caatatattt ggcaaatcag
    12301 tcagccaatg gggaacgtga cacaattgtc ttcagttttg caaagtgcat cagcggcaac
    12361 gaaacgtgta ttcgagattt tagatgagcc agaagaaaaa gaaaacaaag tagatgcttc
    12421 actgccagaa acgattttag gagatgtgac attcgatcat gtcgattttg catatgatcc
    12481 gaagaaacca ttaatccaag atttgaattt tgaagtgaaa gctggtcaaa ccgtagctgt
    12541 ggtcggtccg accggtgccg gcaagacgac attgatcaat ctattgatgc gtttttatga
    12601 cgtagacaaa ggagcaataa ggattgatgg aatcgacacc aaatcaatga gtcgcagtga
    12661 tgtgcgttcg ctatttggta tggtcttgca agatgcatgg ctttatcaag gaacgattgc
    12721 agataatatc cgcttcggaa agcttgatgc gacagattat gaagtagttg atgctgcgaa
    12781 aacagcaaac gtagatcatt ttatccgaac gatgccagat ggatatgata tggccatcaa
    12841 ttctgaggga gacaatgtgt ctttaggaca aaaacagttg ctgacgattg cccgagcagt
    12901 ggtctccgat cctaaaatct tgattttaga tgaagcaaca agttcagtag acacccgttt
    12961 agaagcgttg attcaaaaag caatggatcg cgtgatggag ggacggacaa gtttcgtcat
    13021 cgctcaccgt ttatcgacaa tccgtgatgc agatctgatt ctagtgatgg atcaaggtca
    13081 gatcattgaa aaaggaacgc atgagagttt attggcacaa ggtggatttt atgaaaaact
    13141 gtataacagc caatttgctg aggaagcaga ataagtcata taagaataaa acagtttaaa
    13201 acttattttt tgttctctat tcttgataat ggtaaagtaa agttatcaag aaaagtgagg
    13261 acacggagat ggaacagttt ttaaatgaaa aaattactga agccaaaaga gaagttgatc
    13321 gtttaacgca cgtctgtcag acgaagcctt ttgattcagc tgattttttt gaaaatgagt
    13381 ttcaaatcga acgcttggca gcacaaatag aagtgtacga attaatttta aaaaaagtaa
    13441 tagaagaaaa tatagatgta caataaaaac aggtaaaacg tcagttacca gatactgtaa
    13501 atagacatct ggcctgagca ggttttacct gttttttttc ttatgaaata aatatatgct
    13561 atgatacctt tattcaatag gattagtttc tacaaaaaat gaaaggagaa aaataaatat
    13621 ggaacggttt atagcatcaa atgccacagt gattggtgat gtgacattgt cagaagatgt
    13681 gacgatctgg tatcaagctg ttttacgcgg ggacagtaat tggataaaaa taggacaaag
    13741 aacgaatatc caagatggta caatcatcca tgtcgatcat gatgcacctg tagatattgc
    13801 ggagaatgtg acggtaggac atcagtgcat gctacatggc tgtacaatcg aaaaaggggc
    13861 gctgatcggg atggggacca cgatattgaa tcatgcggtt attggtgaga atagtttgat
    13921 tggtgctgga tcattagtaa cagaaggaaa agttattcct cctaatgtct tggcttttgg
    13981 tcgcccagct aaagtgatcc gtcctttgac aaaagaagaa atacaaaaaa acaaagaaaa
    14041 catccagcat tatgtcgaga taggtcggat gcatgcgcaa ggaaaattca aggagcaaag
    14101 ataggcagta gcatccataa aataaaaaag gatggtcagt ttgagttgac catccttttt
    14161 tatttggttt agataagaga gcttaaaaaa gcttcctttg aaacagatta atttaatgaa
    14221 gaactgtttt ccatgacttc atcgatcaat ccatatgctt ttgcttgttc agctgtcata
    14281 tagttgtcac gatctgtatc tttttcaatc acttcaagcg gttgtccagt gcgttcagct
    14341 aaaattttgt ttaagcgttc gcgtgtttgc aaaatatggc gtgccgcaat ttcgatttca
    14401 gttgcttgcc cttgagcgcc gccaagaggt tgatggatca tgatctcagc atttggtaaa
    14461 gcaaatcgtt taccttttgt accagctgtc agcaagaagc ttcccattga agcagccata
    14521 cccataacaa ttgtttggac atctgctttg acaaagttca ttgtgtcgta aatagccata
    14581 cctgctgtaa ctgaaccacc aggagaattg atataaagat agatatcttt ttcagaatct
    14641 tgagcgtcta agaaaagcaa ttgggcgatg atcgaattag caagatcatc tgttacttgg
    14701 ccgcttaaca tgatgatacg gtctttcaat aaacgtgagt agatatcgta agctctttca
    14761 cctcgtgaag attgttcaat gactgtagga attaaattca taatagagtt cctccttgat
    14821 ttcgtttaga aatagtgtac cataatttag tttctaatac ctaaaatgta gtatacattt
    14881 ttggtcaaaa aaggtcaaat aaaaaactta ggcaaattag tcgcataaac ccgctttatg
    14941 gtatgataag cagagaaaca aaacaaggag gaacaacaga ttgatagttg gtattactgg
    15001 ccttatcgga gtgctgacag gtgctgtagt tgtctatatc ggcatgatac tgcaaatgcg
    15061 tcacttggaa aataaagaac agcgtagacg tgaattggaa ttgaaagtca aagaaatcga
    15121 gacgctgaat caattaaata aaaagatcaa cgagatcttg caaaaacgga caacgctgat
    15181 ggaagaatat gtcagctttg atgcatttga tgattgttat atcacgattg acgattttgc
    15241 gtatctccaa tcattttcag cgcaaaatag tttttatctg ccaaattata ttttagacga
    15301 gctatttaag aatatctccc atcgaaaagt gattttatct ccagatgaaa caatgaagat
    15361 tggtggatat gcttataaag gcggacgcgt gattttagag aattttacgg atagtctgac
    15421 tgaaatgatc aatgaaaaaa aagctcagtt gaagactatg acgcgccagc cgattggttt
    15481 cttctcgaaa gatgatgttt ggagctgata ttttgaagca ttgccgttgc acatgagagc
    15541 gtggtaatgc tttctttttt tcttaatgat acaatagaac tgaacggagt gaagaaagat
    15601 gttacaaatc tatgcccatc gcggaagcag cggcacccac ccagaaaata cgcttcctgc
    15661 atttgcagaa gctgtacgag taggtgcaga tggtatcgaa ctagatgtcc atctaagcaa
    15721 agatggttat ttgatcgtta tgcacgacga agaagtggac cggacgacta acggtaaagg
    15781 gctgattcga gaaaaaacat tggaagaact caaaaaattg aatgcagggg gcaaattcag
    15841 caaagaattt ccagcagcta aagttcctga attgagagaa gtgttaggac tgctcctcca
    15901 aaaaaattac cgagggatgc taatgattga aattaaaact gatcagtatg aatatcccgg
    15961 tatcgaagaa aaattagccg aaacattaaa aagtagagat tggccttttc aacatgcata
    16021 ttgcagcttt catttggatt ctttggaaaa aatcgcatta ctggaaccgg agatacagct
    16081 ggacttattg atgagtactt ctgataaaaa gatccaacta gcagcagaaa ccccttatat
    16141 agaaggagta catccaaaaa atacttggtt gttcgaacat ccagtccagg cagctgattt
    16201 tccaaaagca attcgtgcat ggacgattaa cgatgaagaa gaaatggcca aatgtcaaga
    16261 actcaaaata gatggaataa taactgattt tccggagaaa gctcttcggt ggaaacaatc
    16321 aacgaaaagt aggtaaaata tggaaaaagt attagtgatc gtcggtccga cagcagtcgg
    16381 taaaacagcg ctaagtgtgg aactcgcaca actatttgga ggagagatca tcagtggtga
    16441 ttcattgcaa atctataaaa atctggatat cggtacagca aaaatcaaag aagaagagat
    16501 gaataatgtt ccgcatcatt tgattgatgt gatcgaacct actcaacgtt actcagccgc
    16561 tgattttcag cattcaggtc gacaattgat taccgagatt gcgaaccgag ggcacctacc
    16621 tatcatagct ggtggtacgg gtctctatat ccaatccctt ctatatgatt atcagttagg
    16681 agcgacagaa gaagcgaatc ctgagattag aaaaaagtat gaaacgttag ctgaaacaat
    16741 tggttcccaa gcattatgga atcagttgaa agaaaaagac ccggcagctg ctgagaaaat
    16801 ccactggaac aatcaacgca aagtgataag agctctggaa gttttagaga cgacaggaca
    16861 tagtatcacg gcacctaaag aagagccaaa gcaattgtat gattacctca tgattggatt
    16921 agatacgaag cgtagtattc tttatgaacg aatcaatcaa agagtcgatt tgatgctagc
    16981 agaaggttta gtagcagaag cgcgtaaggt gtatgagctt ggagaagtcc aggccagtca
    17041 aggtattgga tataaagaat ttttccctta ttttgaaggg aaagaaacat tggaagaagc
    17101 aacagaacaa gtcaaactac attcgagaag atacgcgaaa cgtcagttga cgtggtttcg
    17161 taaccgtttg gatgcacagt ggtttgatct tataagtgag ccaaatcaaa aaagtcagat
    17221 tgagcaaaca atcaaaaaat ggctggaggc ggaataaaat ggaagaaaaa gtgatcatcg
    17281 taggggtaga aaccgaagca aaccagcgtt acttttcaga gtcaatggaa gagctagttc
    17341 agctgacgaa taccgcatca ggagaagtcg tattcacaat tacacaaaag agaccacaag
    17401 tggatcggca aacgattatc ggtaaaggga aattacaaga attagttcaa caagcggatg
    17461 cgcatgaagc ggatttgatc atctttaatc atgagctaac tccgagacag agccaactga
    17521 ttaccgatgc tgttggtctt ccggtaattg atcgcgtgca gcttatctta gatatttttg
    17581 cgatgcgtgc tcgttcgaaa gaagggaagc tgcaggttga acttgctcag ttagaatacc
    17641 tgctgcctcg tttagtaggg caagggaaaa cattatctcg tcttggcggt gggatcggaa
    17701 caagagggcc aggtgaaaca aagttagaaa cagaccgccg acatatacgg aataaaattt
    17761 ttgctgttaa gaaagaattg aaagaagtag aagctcatcg agagcgcaat cgccaaaaaa
    17821 gaaaaaatag cgagattttt cagattggac ttattggcta cacgaatgcc gggaagtcaa
    17881 ctatcttgaa tctattgaca caagcggata cttattcgaa agatcaattg tttgcaacgt
    17941 tagatccatt gactaagaga tggcgttttg ctgaaggatt tgagattact gtaacggata
    18001 ccgtcgggtt catccaagac ctgcctacgc aactgatcga tgctttccat tctacattag
    18061 aagaaagtca aaatatggat cttttgctac atgtagtcga tgctagttct ccagatcgta
    18121 ttttacagga gcagactgtc ttaaaattga tggatgaact gaatatgaaa gaaatgccga
    18181 tactgactgt ttacaataag gcagaccaga tcgacccagc gatgttcacg cctacgctat
    18241 tcccaaatgt attgatttct actcagacga aagaaggtaa gatggcgttg atcgaagcaa
    18301 tcaaacgtca gctgatggaa ctaatgaccc cttatacact ttctgtgcca agcaacgaag
    18361 gacagaaatt aagcgagttg agaagacaaa caatggtttt ggacgaagcg tacatggaag
    18421 aaacaaacga atatgaagtt aaagggtttg caaaaaccaa ttcaaaatgg ttaagaaaaa
    18481 accaataaaa ttttattttt ttaa
//
LOCUS       DAZBTF010000056        18169 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000056 DAZBTF010000000
VERSION     DAZBTF010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.056"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(39..863)
                     /locus_tag="ACHGNS_001997"
     CDS             complement(39..863)
                     /locus_tag="ACHGNS_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFN6937927.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(836..1516)
                     /locus_tag="ACHGNS_001998"
     CDS             complement(836..1516)
                     /locus_tag="ACHGNS_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFN6937928.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1539..2477)
                     /locus_tag="ACHGNS_001999"
     CDS             complement(1539..2477)
                     /locus_tag="ACHGNS_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFN6937929.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2722..3573)
                     /gene="ispE"
                     /locus_tag="ACHGNS_002000"
     CDS             complement(2722..3573)
                     /gene="ispE"
                     /locus_tag="ACHGNS_002000"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFN6937930.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(3727..6300)
                     /gene="mprF"
                     /locus_tag="ACHGNS_002001"
     CDS             complement(3727..6300)
                     /gene="mprF"
                     /locus_tag="ACHGNS_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFN6937931.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6441..7247
                     /gene="proC"
                     /locus_tag="ACHGNS_002002"
     CDS             6441..7247
                     /gene="proC"
                     /locus_tag="ACHGNS_002002"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFN6937932.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7326..8882)
                     /gene="rny"
                     /locus_tag="ACHGNS_002003"
     CDS             complement(7326..8882)
                     /gene="rny"
                     /locus_tag="ACHGNS_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFN6937933.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(9293..10342)
                     /gene="recA"
                     /locus_tag="ACHGNS_002004"
     CDS             complement(9293..10342)
                     /gene="recA"
                     /locus_tag="ACHGNS_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFN6937934.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(10451..11692)
                     /locus_tag="ACHGNS_002005"
     CDS             complement(10451..11692)
                     /locus_tag="ACHGNS_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFN6937935.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(11932..12510)
                     /gene="pgsA"
                     /locus_tag="ACHGNS_002006"
     CDS             complement(11932..12510)
                     /gene="pgsA"
                     /locus_tag="ACHGNS_002006"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFN6937936.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(12719..13642)
                     /locus_tag="ACHGNS_002007"
     CDS             complement(12719..13642)
                     /locus_tag="ACHGNS_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFN6937937.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(14381..15667)
                     /locus_tag="ACHGNS_002008"
     CDS             complement(14381..15667)
                     /locus_tag="ACHGNS_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFN6937938.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(15660..16928)
                     /locus_tag="ACHGNS_002009"
     CDS             complement(15660..16928)
                     /locus_tag="ACHGNS_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFN6937939.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            17180..17641
                     /gene="mscL"
                     /locus_tag="ACHGNS_002010"
     CDS             17180..17641
                     /gene="mscL"
                     /locus_tag="ACHGNS_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFN6937940.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(17700..18146)
                     /locus_tag="ACHGNS_002011"
     CDS             complement(17700..18146)
                     /locus_tag="ACHGNS_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6937941.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT         5178 a         3969 c         3175 g         5847 t
ORIGIN      
        1 aaatttttcg tttttataag gcttcttttc tttataattt agttattttt tcttctgaag
       61 gcaaccaata ttcgtttgat gatatttaca atcaggaaca aagcaataaa gactaatgtg
      121 attgttgctc ctggaggtgt atctagataa aacgagctag ttagtcctga cagcatccct
      181 agaaatccta cacaaacact gatagcaatc acgacattga agcttttgcc taatcgcata
      241 ccgattgctg cgggaagaac catgattgcg gatatcaaaa gtgcaccggc aatcgggatc
      301 atcacagcaa tagccacacc agtaatcaca ttgaacatca tcgacatcaa atgaacgggt
      361 agcccatcca cgtgggcggt atcttcatca aaagtcagaa catacatcgg ccgtttgaat
      421 aaaaagaaca atataccgat ggcaacgaat aagattccta aaaagatgac ttgctcccat
      481 gtaatggtca cgatcgaacc aaacagatag gattggatac ttgttgctga attccctccg
      541 ctcaaattca tcaatactaa cgctagcgcc aaaccaccag ccattaaaat ggcaatggag
      601 atttctgaat aggttcggta gatcgttcgc agatactcca atatcacagc tgctaataca
      661 acgactagca tggtcgtaag tgtaggatta aggttcaaaa agaatcctaa cgctacccct
      721 gctaaagaga catgagaaag cgtatcagcc atcagcgatt gtcttctgat cactaaaaac
      781 acacctagca tcggggcgat tccggcaatg aagatagcgg caagaaacgc tcttctcatg
      841 aattcatatg aaagcaacgc cattgagaat cctcctttct caccagtcgg atctgtctgt
      901 ccgcatactg tttgatatct tcatgatcat gggtgatcat tagaactgat ttcccatgga
      961 catgcgcact atgtcggaga agacgataaa attcatttct ggattcttca tccatacctg
     1021 ttgttggttc atcaagaaca aataagtcag ggtcagtagc aaaaatacgc gctaaactga
     1081 ttcgctgctt ttgtccgcca gacaattcac ctattcgccg atttcgcata tcccacatcc
     1141 ctacagcttc taaagcacgc tgtacatgtt gatgatcttt ttcagagaga cgcttgaacc
     1201 agcggcctct tggatatctt cctgaacgga ctaattcgat caccgtacta ggaaaacctg
     1261 cattgaaaga tgcaacttgt tgaggaatat aaccgatact taattttttt ccttcttgat
     1321 ttttctcagc aatctttatc attccttttg aaggtttcag taatcctaac gatgctttca
     1381 ccaaggttga cttggctgct ccattttctc cagttaaaat gacaaattca cccgcatcta
     1441 catgataaga gacatcttct agtacaggtt catcatcata gtaaaaggtc aaatcagata
     1501 tttcgatata acgcacctta caacctccat tctaaaactt aattgatact ttcttttaaa
     1561 gctttcagat ttttttccat gacagaaata taattttcgc ctgctttgat ttgttcatta
     1621 gtcaagcttt ctaaaggatt caacacttct agcttcactc ctgtttcatt cgacaatgtc
     1681 tcagctacct tcgaagaggc attttcttca aaataaatca ctttgatgtt gtttttcttc
     1741 acaaattctt ttagctcagc taagcgactt ggtgtgggtt cctgatctgg tgaaacgccc
     1801 gaaatcgcta cttgttttaa gtcgtattga ttcgctaaat aagcaaaagc agcatgctga
     1861 gtgacaaatg tgcggtttgt tgcatctttt agctcatcgg agtatttttg atccagtgct
     1921 tcaagctttt tgatataagc atcggcattc ttcgtaaaag tttccttttt atctggatat
     1981 ttttcgccta attcttcagc aatcgttctg acttctttta tagctaatac cggatcgagc
     2041 caaacatgtg gatcgtattc atgtgaatga ccatgccctt cttctgattc ttcatgttcg
     2101 tctgcttctt gcgattctaa acggtcgatt cctttagctg cttcgatcat caaggtctgt
     2161 ttgctatcca ctgaatcctt taacgaatcc acaaaaaatt ccatgtctga actattatac
     2221 acgaaaacat ctgcatcgga aatttctgct ctttcttttg cacttggttc gaaatcatgc
     2281 ggctccgttc cagcagggat caaaagtttc acgtttcctt catcccctac gatctctttt
     2341 gtaaaatcat acatcggata aaaagtcgta acgatcgtca aatcctcttt tttgtctgct
     2401 tccttgttcg tatttccaca tgctgcaaac agtaaagcac ctatcattac cgtagctgtc
     2461 aaaagatatt ttttcattta tatgctcctt tcaatgaaca aatcggaatt ttacgattta
     2521 ttaacgtgaa ctaatgtatc aaaataaaaa aacacagtca atagaaatcc atcaatttct
     2581 gatttaagca tgtatattct tgattaaatc gtaaaatgga tgagttgctg ctcatatcta
     2641 gccatttcac tacaataaaa aactgaagaa taccatttta ggattcctca gtttttcatc
     2701 acctatttta cttcttatat tttattgcag ggttcgtact aaataaactt cctcacaaaa
     2761 accttttaac ccattatata ctctctgcgc tcttgaataa tgttgacaca aggcaaaaac
     2821 ggttggtccg cttccactca tcaatgcagc atctgctccg tacttgatca tacgctcttt
     2881 gatttgatgg atgactggat gccgctttcc cgttatatct tctaaactgt ttcccatttt
     2941 tttgatcatc tgctgataat cttgctctcg gatggcttca gcaagtccgt ggatgtcttg
     3001 atgttggatg gcttgcgtat ctacttctct aaacactgta cgtgtagaga cactcattct
     3061 tggtttgacc aacacaaccc agcactgtgg catagaaggt aatacttgga tttcttcccc
     3121 tttgccaccg acaaatgccg tacttccgta gacacagtaa ggaacatctg taccgacttc
     3181 caaaccgatt tctgccagtt cttctaacga acagcccaga ttccataatt ggttgatccc
     3241 tcttagagca gctgcacagt ctgtacttcc tccgcctaat ccagctgcta ctggaatatt
     3301 cttcttaaca aagatcttga caccggtttt gatattgtat ttatttttta gcaataaagc
     3361 agcttgataa acattgttcc gttcatctac cggtaaaaat gctttatttg tttctacaat
     3421 gattttgtct tctgttattt cttcaaaaaa tagatgatct gccaagtcta cgctcgacat
     3481 caccatttcc aactcatgat acccatcttg ccgtctatat aatacatcca gtcctaaatt
     3541 tatcttggct ggcgcttttt caatcaattc catcctctca cccattcgaa aagtctaagt
     3601 cgcaagtatt ctatcatagg gaaaaataaa agtatagctt atgtctgcct agtggttgat
     3661 tttgtcatcg tttcaacagc tttcacagaa ttttctcttc ataatccaaa caagttctag
     3721 tgattcttat aattctgtga gcacttcttc tgccaaactc ggaacatttc ctttttcttt
     3781 tcctatcaga ctgagtagct gcatggtagc aaatccgaaa ttacttcctt tcctataagc
     3841 aaaataacgt ccttcccaat gatcgacata ttttaatttc ttttcataag cagactggaa
     3901 ggaatagaaa aaattaccat aattatatac gatatgcatg actttttctt gaaagaaaga
     3961 aaaatctgtt tctccaacat tagctaatgg agctaagcct agattggctt ccgagtagcc
     4021 cattttctcg tatacaataa acagattggc taacagatag tcagctaatt cctcagattc
     4081 tttaggtaag acccgcaaca aatcatatcc cgcctgcttc ttcgtcatcg gctgagcggt
     4141 gatgaagcca acgacttttt gatctttacg gacaataccg acaccgctag ataatacata
     4201 tgatttatca aatcgtccca cagagaagta tttttctcgt gcaccttgaa gccatttttg
     4261 tgaaacttca tttacagctt gatgtaattc ttccggcagt tgatcgtacc aagtaaaatg
     4321 gtatccttct ttcatcaaac gttgatattc aagttgctga ctcagccgag acaagtcttc
     4381 tgttgctaga tcaacaatcc ctgcttcacc tactttcgtg aagtgaaaac ccaattcatg
     4441 aagcatcact acgtagcttt ctgatatctt ataaaacgca agctgatagc ctaaacaatc
     4501 tgcttgtttc ataaactgat tcgttgctgc ccgcatcatt ttaggattgc caactggatc
     4561 tcctaaaaca aagcatttgt ttcctttgat ctgataacca aaaacgactt gatcttggtc
     4621 gtcttcttgg taataaaaat aactgtaacc tgacaagcga agctgatggc ttgtagctgt
     4681 gcctccgtat gtttccaata aacggtcaaa acggtctttt tcgtactcac tgccaagctt
     4741 cgtagtatct tcagctaaat attgataaag cgccactaaa gctagcaatg agatgcccaa
     4801 accaataagc cctgagaacc acacatcatc tgaaggaaac aaaataaaat gatttcctgt
     4861 tattcgaccg actaaaaagc gtccgctgtg atagcctgca attgcgtaaa cgataaacaa
     4921 aaagccgaac aagctagcat caaagattat agctccccaa gaataaacga attttgttcg
     4981 ataaaattct tttctggata accacacagc taacagaatc aagacatata ccaatatcaa
     5041 ctgccaagat aacgtacgtg tgattgtatt taaaatacca aaagatagta aagcaatcgt
     5101 aggcagaaat gcctttttga cttttactga aagtgctcta gcaaggccga gtaataaaaa
     5161 gccaaccaat agattcactg tctgatctaa aaaattgaat gagaacggca ataatacttg
     5221 aaatagccga ctgattgcag acagattagt gattgttgat agaagtacca tcatgatccc
     5281 cgcaaaatag acagcactta cgagcacgat atgcgcaacc ttttggaaaa acagtcgcgg
     5341 aatgttatct aaaaatcgat tgattttgac tcctgtatgt gtgatgaaca atagaagtcc
     5401 cgtcataaaa ggtagaacat aataaaacaa ccgataatat aatagccaaa ctactgctac
     5461 atcttgccga acagacaatt gactcattcc taagatcatc aaaacgtcaa aagtccccat
     5521 tccacctggg accatcgtca gcattcctat caatgtagca atcatgaaca taggatagat
     5581 cgaaagcatc gaaatatcta ttctcatcaa cttgccgatg atcaaaaata ccgcaagggc
     5641 tcccgtccat tgtccaaagg atgccagtag cagaccaagc aagccttttg gataaaactc
     5701 gaagaacaat gtctttctct ttagataagc aaaaaagaaa atgactggtg ctaaaaaact
     5761 accgccgaaa agccagatcc aatagcttcg aaaaatactt tctgactgaa taaaaaagac
     5821 atcaataaat gtaagaaacg accatacaga caaaccagtc agcataaata gtgcgacttt
     5881 tgatacagtg gctaaaacca tttttctatt tgtctgcttt ccataaaaac tagcccgcag
     5941 acttgctcct accacaccac caaaaccggc gagattgttg atcgtgtttg ttacccacgc
     6001 agcaagaaga aactcttttc tttccatttt tggttttcct tgtttttcta agactctgat
     6061 cgtcacccaa tcatacaaca acatcggagt gacacccagc agtccagcca gaatcatgag
     6121 caggatcgtc atgcggcttt gttgtcccat cctttgccaa atatcttgcc aactcatgcc
     6181 attagcaata tttgcgactt gatttgccac aaagactaac acggagccga aaaaaatcag
     6241 ttttaaaatc agactatgtt cctttatcca ttgcagcatc aagtatcgtt tatttttcac
     6301 attttcccat tcctaacttg tatacttttg tgtcataccc caatgttatc ttatttattt
     6361 taatttttcc actttagacc tgttctttgc ttgcgatgat agaaaaagaa agatatccta
     6421 atgctaaagg agaggataac atgaaagtag gatttatcgg cacagggaat atggcacaag
     6481 ctattattga tggtttatta aaaaaaggaa cagtctcgaa agaagaggtg ttcgtttcca
     6541 gtggacattt tgaaaatgca caaaaatttg ctgctgaaac tggcgtccat gcatgcaaaa
     6601 gtaatcagga agttgcatca caatcagata tcgttctttt agccgtgaaa ccaaaaatca
     6661 ttgaaaaagt attggaagaa ctaagagaat atagccagaa agtcttattc atttctattg
     6721 cttcaggaaa atcattaata gatttagaaa cagcgcttga agatcctcag gcctccatcg
     6781 tacgtgtgat gccgaatgtc aatgtcagcg taggagcagg cgtttctgcg atttgcaaaa
     6841 atgaagctgc cacggaggaa cagcttcagc aagctacgat gatttttgaa gcaatcggca
     6901 gcgtctatcc tttagcagaa tcagatttca gtacattcat tggccttgct ggcagctcac
     6961 ccgcattcgt ttacatgctg atcgatgcga tgggacggac tggtgtactg aatggtttgt
     7021 ccaaacagac agccactgag attgctgcaa aagcaattat gggttcttgt caaaaattcc
     7081 tttcaagtga taaaaatcca tgggaacttg tagaccaagt ttcttctcct ggcggtacaa
     7141 ctgttgcagg tgtagttgcc ttagaagaag caggattcat cccagcaatc atcaaaggaa
     7201 tcaatacgac gatcgagaaa gatcttgaga tgttgaataa aaaataatga tagcaatgtt
     7261 ttttcgtgcc ttacaaaaaa agaagagctg tggcttttgc cgcggctctt cttttgtatt
     7321 ttttattatt tagcataatc agttgcacga gtttcccgaa taacgattac tttgatgtgt
     7381 ccaggataat ccagttcttc ttcgattcgt ttacgaatat cacgtactaa acgaacagcg
     7441 tctaaatccg agatttcttc tggcttgacc attacccgaa cttcgcgtcc agcttgaact
     7501 gcgtaacttg attctacacc ggcgaagctg ttcgaaatat tttccagact tcttaaacga
     7561 cggatgtagt tttctaatga ttcacttcgc gcaccaggtc ttgctgctga gagcgcatct
     7621 gctgcagcta caaggacaga gataacagaa gtagcttcca catctccatg atgagaagcg
     7681 atcgcgttga ttactactgg gttttctttg tacttggcag ctaattccgc accgatctca
     7741 acgtgagatc cttcaatctc atggtcaagt gctttcccaa tatcatggag caacccggca
     7801 cgttttgcta attggatatc ttcgccaagc tctgctgcta agataccagc taattttgca
     7861 acctcgaccg aatgcttcaa gacgttttgt ccataactag ttctgaatcg cagacgtccg
     7921 atgatcttga ttagatctgg atgaagtgta tgagcaccga cttcaaacgc tgcctcttcc
     7981 ccgtattcgc gaatacgttc atccatttct ttacgagatt tctcgaccat ttcttcgata
     8041 cgcgctggat ggattcgtcc atcttgtatc agtttttcca aggtcatccg tgcgatttct
     8101 cttcggattg gatcgaatcc ggaaagaaca actgcttccg gtgtatcatc gatgattaaa
     8161 tcgataccag tcagcgtctc aagcgttcga atattacgac cttctcgtcc aatgatacga
     8221 cctttcattt catcattcgg aagggtaacg actgaaacag tcgtttctga aacttgatct
     8281 gctgcgcagc gttgaatcgc taatgataat aagtttttcg ctttgcgatc tgcttcttct
     8341 ttggcacgct gttcagattc ttttaccatt agagttaact catgattcag ctcttcttca
     8401 gtcgatttca tgatgacatc tcgtgcgtcc tcttttgata aaccagcaat tttttctagt
     8461 tctttttgtt gatcttcaat cattttttca acgtcttttt ctcgctcttc aattaattgc
     8521 tgttttgaac taagtgcttc ttctttactt tcaagtgagt tctcgcgttt ttctaacgag
     8581 tcatctttac gattcagcgt ttgttcgcgt tgaatcaaac gattctcttg agattttaat
     8641 tctaacttac tttctctcag ctcactttca acttcagaac ggtatttctg attttcttcc
     8701 ttagcttcta acaatgcttc tttttttaga gtttctgctt ctttttttgc actatcgatg
     8761 attccagcag ctgatgattg tgcaccagct atttctttct cgtgacgtga ctttgcgacc
     8821 ataaacccta aaccaagacc gacaattaaa ccgatgatag cgaggagaat gttgaatacc
     8881 ataaaatcca cctccatact atctttttca tttttatcat ctatacagct tctatgataa
     8941 attattctaa actcgtcaat atgcatcttc gcatatatgt gtacaaaaaa catgcacaac
     9001 tttattctaa tgtttgtacc ccgtagtgtc aactttaaat aaacaaagaa acggctttct
     9061 acttattcat taaacgatgg tttttaaaat aaatgacact agtttcatat aggtaaacct
     9121 ttaaattgag aaacttttct attaaattat tctttgtaga tgaacagata tttttatttt
     9181 atgtacccac tcgtataatt aaataactca cgatttcttt attcaataaa aaaacggcag
     9241 gtcaaggaga gtacctactc ttcagaccag ccgtttttgt ttatcataat ttttattctt
     9301 ctactaaagg caattcttct tgtccttttt catcttctgg gacagcgact tcttcaccaa
     9361 taccataagc tgctctaact agagcagaaa cttctgccat catttcagga tgattagcca
     9421 tatagatttt agcgttttcc cgtccttgac caatccgatc ttctttataa gaataccaag
     9481 caccgctttt atcaacaatg tctttttcta ctgccatgtc tagaagttct ccttcttgtg
     9541 agatccctaa accatacatg acatctactt cagcgatttt gaaaggaggt gctactttgt
     9601 tttttacgac tttgatcttt gtacggttac caacaatatc tgtaccttgt ttcaattgtt
     9661 ctgcacgacg tacttccaaa cggatcgttg cgtagaattt caatgcgcgt cctccaggag
     9721 tgatttctgg attcccaaac atcacgccta ctttttcacg gatttggttg atgaaaatag
     9781 cgattgtttt tgttttgttg atcgaaccag agagtttacg caatgcttga gacatcaaac
     9841 gtgcttgtaa cccgacatgt gagtcaccca tttcgccgtc gatttctgct cgtggaacta
     9901 aagcagcaac tgaatcgact actacgatat ctacagcccc acttgatact aaagcatcag
     9961 cgatctctag accttgttct cctgtgtcag gctgtgaaag aagtagttca tcgatattca
    10021 cacctaattt ttgtgcatat tgcggatcta acgcatgctc agcatcaatg aaagcggccg
    10081 ttccgccatt tttttgtact tctgcaatag cgtgtagtgc aactgttgtt ttacctgaac
    10141 tttcaggtcc atatacttcg atgatacgtc cacgggggta accgccgaca cctaaagcga
    10201 catctaacgc aagagagcca cttggaattg tagagatttg ttggtcgatt ttttcgccca
    10261 atttcataat agagccttta ccatagctct tttcaatttt tttcagtgca gcatctaagg
    10321 ctgctttacg atcatctgcc aatacgataa tcctccttat atttacaaat cagagcaaca
    10381 tttcattatc cttatcatat cttttttagt gaaaaaaagc aagaaaaaac cgaacaaata
    10441 ttcgcattta tcattttctt aaaagctgtt ttctcaaaag atcaaatcct tgcataaccg
    10501 cattatatct gatagcagca cgatctctgg caaagtgata tttttccgaa accacgccgt
    10561 ctttagatga aatagcgatc catactgttc ctgcagattg accttctaac gtgtccggtc
    10621 cagccactcc ggtgaaagct acactgtaat ctgttcttgc tatctcacgt gcttgaatgg
    10681 ccatctgctc cacacattct ttgctgactg ttccatattc tgctaatagc tccggatcaa
    10741 tctccagaaa accagctttt gtttggagcg agtaggtaac gaatccgcct ggaaatactt
    10801 cagatacacc agcaatgtcg cctaatgcgg cttggaaggc gccggctgtc aaactttccg
    10861 ctgctgttac agtctgcttg ttttctttca gtagttccac tacgactttt gctagcgaat
    10921 tatcatcgcc gtatccataa aaatattccc ctactcgttc ttggattttt tcttctaatg
    10981 ctaaaagagc ttgattccct gcctgaacat catttgtttt cactgtcagt cgtaatgtca
    11041 cttcatttgg tttggcatat ggtgcgatcg ttggattgat ctgtgtctca atcagatctt
    11101 ttagctcagt gaccagtcgc gattcaccaa tcccataaaa gcgcaaaact ttagaaatta
    11161 gtttttcttc agaaggaaaa cgttcctcta gtaaaggacg tacttgttcc acaaacattg
    11221 gtttcaactc attaggcgga ccgggaagaa ggatatacgc atgtgtatct gtttgataaa
    11281 aagtccctaa agcaagccct gtacgatttg gtagaggaac cccgccttcg ataatctggg
    11341 actgttgcag attgtttttg gtcatttttc gatgggttgt ttcaaaatat gccaataatt
    11401 ttttgtatcc ttccgtattt tgtatgagag acttaccgag gtgagccgca gttgcttctt
    11461 ttgtcaggtc atcttctgtc ggtcctaatc cgccacataa gagtatgagt tcacttcttg
    11521 tttcagctag agcaatcaac tcttctaaac gctgctgatt atctcctaca actgtttgat
    11581 aataaacttc gatacctaga tcagctagct gttctgacaa gaaagtggca ttcgtattca
    11641 cgacttgtcc tagtaaaagt tctgttccga cagctattat ttctgctttc attacttccc
    11701 gctcccttat atagtatacg ctgtattttt atattttttt atttaaaaag agggcaagga
    11761 gctgtgacaa aaatcttgtc acagctcctt gttccaaata aacagtgttc tcctgccaaa
    11821 acctcggtca taagtcacaa tatacataaa acatgaaaag ctgttttttg aaattatccc
    11881 ttgtgactca gagttagacg gcaggctcac aacctctcca ctaaacgata cttacattga
    11941 tcctttgaac acaccgctgt ttttgatgaa gtaatccaca cctgaataga tagtaaagat
    12001 cagacaagca taaagcatga tttgatctac tggaataccg atcaggctga atgggatatt
    12061 attgataaac agcaagataa tagcaaccat ttgtgtagcc gtcttgactt ttcccggcca
    12121 agctgcagcc atgacttctc catgttcgac aagtagtaaa cgcaagcctg tgacggctaa
    12181 ttcacgacag acaatgatcg ctactaccca accaggggcc tttccttggt caaccaacat
    12241 gataaaagca gtcataacta gcattttatc tgccaacggg tccgcaaact tgccaaaatt
    12301 agtgacaagg cctcgcgcac gggcaatttt tccatctaac cagtctgtga tactagctaa
    12361 agcaaaaata actgcaccta ctagctgtgt gacagccagt gtggtgtctc ctacgttaaa
    12421 ttgtccccaa tctaaaggta aacctacaac caaaatgaaa atcgggatca tacaaattct
    12481 aagtacagtt aatttatttg gtaaattcac actatcgact cctttcttcc ctattctacg
    12541 tgactatcag cgaagtttcc accgatatta tttacttttc caaatgaatt tattcaaagg
    12601 agtattttta ttgaaattga tctttttgaa ttaacgaaga aggaccgcga cacagatttg
    12661 tcacgatcct tcttttttat acctacaagt aaacagatgc gtaaactaat ttatacgatc
    12721 attctgcgta ttgaagagta aggttcacat tttttggact ctgattgttt tcccctggat
    12781 taactggtac ggattctcca ttcgctttga tcgttgcatt gcttgctgca ccaatcgtga
    12841 tggtcgcatt tgttgctgtt tccggtaaca cggtactctg tgtttcattt gctgccagtg
    12901 tcccttgata aacataggca ttgtctacta gcacacctaa ccatacacga ttgacagcag
    12961 taaattcaag ggttacaggc ttggtagcgt ctgtgatatt tactgcgatg gcactgccag
    13021 aatcttctcc tgtagctatt gccattttct tttcttcact tgattttgtt gttgattcta
    13081 ctgtagaaga ctctttggtc tgcgtgcttt gctctgagga agaggactcc tcgactgatc
    13141 cttctacttt aagcgaagaa gctgtgtctc cgattattgg atcagcttga cgatcttgcc
    13201 aagtcatata gcccacgaca acgacaatcg ccaaagcaac caatcctaat aaaatgacag
    13261 gaagacttgt ccaaaagcga cttggatgcg attcttcctg atgcattgct tttctagaag
    13321 tacttactgt ttcaggctgc ggacgttcag ggaccgatcc tgtcaatttg cttttcccat
    13381 caaaaacatc gactaaatgg tctccatctt ctcctacagc ttcagcgtat tgccgaataa
    13441 atgcccgcac gtaaaattta ccaggcaatt ggtcaaaatc atcattgtcg attgcttcca
    13501 aataacgttt ttgaattttt gtaatctgct gtaattcatc taaagatatt tttttattca
    13561 ggcgtgcttg ccgtaatttt tctccgatgg tttcaaaagc cactcgttca tcttctccag
    13621 tctgtttgat tctccgaagc atctgctgtt gcaacgaaag acgttatgtt tgggtgctgt
    13681 gacaaaagtc atgtcacagc tccttttttc cgaataaaca ttgttctcct gccaactcct
    13741 cggtcacaag tcacaatatc caataaatat aagaagctgt tttttgaact tgttccttgt
    13801 gactcggagt taaacggcag gctcacgacc tctttcttaa acggtgttca atcagctgtt
    13861 cctcggtatt agctcaaaaa ccggcaaaat ataaggagat atttttccgt atgtttctct
    13921 aatactcagg tcaaaacgct gatttcacaa cctcttcctt aaacggtgtt caaaagcttg
    13981 catctgcggt aataagtcca accaagttaa aaacattaag aagctgtttt tacttggctg
    14041 ttcttattac ttgatgcagg gcagcttttt cacaacctct tggtacacgg tgttccagaa
    14101 gtagcttctt cggcaataag ccgaaatatc acaaaaattt gagaagcaat tttcgtaaat
    14161 tccttcttat ttcttgaagc tgaccacttc tgtcacaacc tctttgatta cttcgtttga
    14221 ttttatttta atataagttg attaaaagag agcatgattt gttttatttt ctcactaatt
    14281 tttccaacac aactaagaat accataaaag ctgattttgg aggaggttcc tactacaagt
    14341 ctaaaacgaa atttaagact tttcctcata aaatactggt ttattttggt ctcatgtaaa
    14401 actcgctgaa ggcctcttca cttataaatg cttccccagc tgccagtacg tctttcattt
    14461 ggatcgaatc gatgatttca ggaagatcaa ataaagttcg atcgccaaat aaactttgcg
    14521 taaattgatt ggcaatatat tcgatcgaat ttaatgattg gaaatattgt ccaagcattt
    14581 tcttcttcag caaatcgaga tttgtttctg acacttccgg atcatcagcg aatccaagaa
    14641 tgatttcttt gactttttct gctgctttct ctggttcgtc cgtatctccg ctgaagtccg
    14701 caaagtgaaa ttcacgatcc aagctgaatt caaaaccaaa gctgtcatcg ataattcctt
    14761 gattgtacat cgctagatag tttcgagaag tattgcctaa aatcatttgg aacaacagat
    14821 tgattgctgt tttatagcgt aacagttctg ttccttcttg cggcaatgtg tctaatcctt
    14881 taataccaag tacaaattta tctcgtgtaa tggctgcttc caatgcactt tgtttgatga
    14941 tttcttttgt gttttcaggg aaataacgta caatttcttg ctttggaggg aaattttttg
    15001 cttcttggtt ttcccgaata agtttcatca atttctcagg ctccatctta ccaactacaa
    15061 ataaaaccat attgcttggc tgataaaacg tccgatagca cgtatacaag tcttgagctg
    15121 tgattttgtc gatactttca accgtgcccg caatatcgat atgcaatggg tgtgtaggat
    15181 acaagttatt tagtatgcca aaaaacatcc gccagttagg gtcgtcttca tacatttgga
    15241 tttcctgacc gatgattcct ttttccttgt ttacagtttc ttctgtgaag taaggtgctt
    15301 gtacaaaatc gatcagagtc gtcagattct tttctacctg atccgtcgta gaaaacagat
    15361 agctggtttt cgtaaaactt gtgaaagcat tggcggaagc tccttgtttc ccaaataatt
    15421 gaaagacatc accatcttct ttttcaaaca acttatgttc taaaaaatgg gcaattccat
    15481 ctggaacttt caccttttct ttttcaccat agggaataaa ttcgttatcg atcgaaccat
    15541 aattcgtact gaacaaacca tatgtcttgt gatagtcatt tttaggcaac aaatacacgg
    15601 tcaaaccgtt tggcagtact tcatgataaa gtgtttcatt gatttgctca tattctgttt
    15661 tattcatttg catttcctcc tgctaaaaag aagattgcct gcaattcgat ttgctcagct
    15721 acttgttgta tctctttgat cgttacgctc tccatacgtt tcaaccattc ttcatcagat
    15781 aattttgttt ccggcagcca gctgtccaaa aaagaagctt cgattgccgc ttgcggagag
    15841 tctagagaaa gcagatattg gtttctcagc attgcttttg tctgagacag ttccaactct
    15901 gttatttcac catttcgcaa actttctaat tgctcgtgaa tcagatgaag tacttgattt
    15961 cggttctttt catcgatacc ggtttggaca ctcatgaatc ctcggaacgt atccacgcta
    16021 ctagaggcat aataagctag actttctttc tctctgacat tcatgaaaag tttagagtga
    16081 ggaaaaccgc caaataaacc gttaaagacc attagcgcaa aacgttctgg ttcatcgtaa
    16141 tacacatttg tctgataagc taggttcagc tttgcttgaa cgattggctc acgtacttgt
    16201 cgttccttca cgatattcac ttgaggttgg gtataaaaca gatctggata aactgtttcc
    16261 cgcgactcaa atggtaattg cccaactgct tcagctgctt tgtccggatc aacatcgccg
    16321 acaacaaaaa tgtcgatttg atcatttgcc atcatttctt gatagtagga cgctagcgaa
    16381 ctagcagtga tcttgtccaa atcttcgact acgcccaacc ctgggatctt ttgttccggg
    16441 ctgttttcaa aataaagttc ttgcaaggca taggaagcaa aagtttgttt atcttccttc
    16501 aaactttcca aatatagacg cagattgttc ttttccaagt caaacgtcaa ctgatcaaac
    16561 tgatgatttt taatatttgg ataaaataaa acctctttta aaaaatctac cgcttgagaa
    16621 aaaaggtttg gatcattgac atattcacca ttcacaaaac tgatccctgc gtttagccaa
    16681 tgtaaatttc ctttttttct aactgataat ccaaagcttg cgccataaag ctctgcaagt
    16741 tgcgcactta actttgtctg atccgaataa ttcaaactat ttgtttccaa catgctgctg
    16801 agaagtgaac gtttcgtgat gacttcttgt tgcagacgcg cagtaaaacg gataaaaatc
    16861 cggatcgttt tgtattgttt tgttggtaag acatgaagat tcacgccttc tgccaattga
    16921 tacaccatat ttatatgctc cttttcacag ttgattgccc gcttactctt ttaatgggtc
    16981 aacctgcctt ttttacttat tcctctgctt gaaatttata agttcaggca attcgccgtt
    17041 ttttctaaat gatgttacac tttatagtat agcatagatg ttttttatca aaagaaacag
    17101 acggctaaaa gcgtatattg tttttttatt ttttattttt cgatatacta aagacaaata
    17161 aaagaaggga gggtcattca tgatcaaaga attcaaagag ttcatcatgc gtggagatgt
    17221 cttggatctt gctgtcggcg ttgttattgg tagtgcgttt acaagcatcg taaaccaagt
    17281 ggtgcaaggc ttgatcacac cactaatagg ttggtttgtc gcaatgatca caggtacttc
    17341 tgatttagaa ggagcacttt caattcttga ctggtcgccg acaaaaggcg tcacatttgc
    17401 gtttggtaac gttattagtg ctatcattac atttctgatc actggttttg tcttattctt
    17461 agttgtcaaa gcggcaaacc gtgcgaagaa tttacgctcc aaagaagagc aagtggaagt
    17521 ggaagaaact cctactgcag aagattatct tagtgacata cgcgatcttc ttgccaaaca
    17581 agtggcagaa gacaatacac atccagtaga gcaaacaaat ctttccgata cttctaaata
    17641 ataaagaatc taaaaaaaag aaagccgatt ttcagtgtcg gctttctttt tttagattct
    17701 taacttgcta cttgttcctt tttcatgtcg atatgtggaa tatcatcttc caaatacaca
    17761 tctgagattg ccttgaatcc aaacgactca taaaaagctt gtaggtattc ttgtgcgccg
    17821 atcacgattg gtttttctgg aaaatttttc tggatcatgt taatcgtttc ttgaacaatc
    17881 tttcttccta aaccatcttt acggttgtcc tgatgaacaa ggacacgtcc aaaatggata
    17941 ctctccgctt cttcatatac tcgcgtgtag gcaaggatct tcccatcagt atcttgaaaa
    18001 aacgtatgcc aagcttctcc atcaatgtca tcaacttctt gataaggaca ctgttgttcc
    18061 acaacaaaaa ctgcgattcg ttctttgacg atcgcgtaaa actctgctgt tgacaattct
    18121 tcaaatttct ttacctgata ttccactaag caaacgcccc ctgcttttt
//
LOCUS       DAZBTF010000057        17690 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000057 DAZBTF010000000
VERSION     DAZBTF010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.057"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            189..419
                     /locus_tag="ACHGNS_002012"
     CDS             189..419
                     /locus_tag="ACHGNS_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFN6937942.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            516..1817
                     /gene="murA"
                     /locus_tag="ACHGNS_002013"
     CDS             516..1817
                     /gene="murA"
                     /locus_tag="ACHGNS_002013"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFN6937943.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1830..2006
                     /locus_tag="ACHGNS_002014"
     CDS             1830..2006
                     /locus_tag="ACHGNS_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFN6937944.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2141..2437
                     /gene="yidD"
                     /locus_tag="ACHGNS_002015"
     CDS             2141..2437
                     /gene="yidD"
                     /locus_tag="ACHGNS_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFN6937945.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2622..3089
                     /locus_tag="ACHGNS_002016"
     CDS             2622..3089
                     /locus_tag="ACHGNS_002016"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6937946.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3174..4058
                     /locus_tag="ACHGNS_002017"
     CDS             3174..4058
                     /locus_tag="ACHGNS_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFN6937947.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4172..4408
                     /locus_tag="ACHGNS_002018"
     CDS             4172..4408
                     /locus_tag="ACHGNS_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFN6937948.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4496..4576
                     /locus_tag="ACHGNS_002019"
     tRNA            4496..4576
                     /locus_tag="ACHGNS_002019"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4530..4532,aa:Tyr,seq:gta)
     gene            4583..4654
                     /locus_tag="ACHGNS_002020"
     tRNA            4583..4654
                     /locus_tag="ACHGNS_002020"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4615..4617,aa:Gln,seq:ttg)
     gene            4779..4949
                     /locus_tag="ACHGNS_002021"
     CDS             4779..4949
                     /locus_tag="ACHGNS_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937949.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVRVKSKRP
                     TNHTKKWMAKNR"
     gene            5169..5876
                     /locus_tag="ACHGNS_002022"
     CDS             5169..5876
                     /locus_tag="ACHGNS_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN6937950.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5935..6579)
                     /locus_tag="ACHGNS_002023"
     CDS             complement(5935..6579)
                     /locus_tag="ACHGNS_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFN6937951.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6705..6992
                     /locus_tag="ACHGNS_002024"
     CDS             6705..6992
                     /locus_tag="ACHGNS_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937952.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7100..7420
                     /locus_tag="ACHGNS_002025"
     CDS             7100..7420
                     /locus_tag="ACHGNS_002025"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN6937953.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7501..8814
                     /locus_tag="ACHGNS_002026"
     CDS             7501..8814
                     /locus_tag="ACHGNS_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFN6937954.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            8811..9530
                     /locus_tag="ACHGNS_002027"
     CDS             8811..9530
                     /locus_tag="ACHGNS_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFN6937955.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            9658..10209
                     /gene="raiA"
                     /locus_tag="ACHGNS_002028"
     CDS             9658..10209
                     /gene="raiA"
                     /locus_tag="ACHGNS_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFN6937956.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10431..12965
                     /gene="secA"
                     /locus_tag="ACHGNS_002029"
     CDS             10431..12965
                     /gene="secA"
                     /locus_tag="ACHGNS_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFN6937957.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13091..14192
                     /gene="prfB"
                     /locus_tag="ACHGNS_002030"
     CDS             join(13091..13162,13164..14192)
                     /gene="prfB"
                     /locus_tag="ACHGNS_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFN6937958.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14297..14983
                     /gene="ftsE"
                     /locus_tag="ACHGNS_002031"
     CDS             14297..14983
                     /gene="ftsE"
                     /locus_tag="ACHGNS_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFN6937959.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14976..15860
                     /gene="ftsX"
                     /locus_tag="ACHGNS_002032"
     CDS             14976..15860
                     /gene="ftsX"
                     /locus_tag="ACHGNS_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFN6937960.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            16012..17460
                     /gene="cls"
                     /locus_tag="ACHGNS_002033"
     CDS             16012..17460
                     /gene="cls"
                     /locus_tag="ACHGNS_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFN6937961.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT         5916 a         2983 c         3838 g         4953 t
ORIGIN      
        1 atttggaata agggactgcg acaagacttt tgtctcagtc cctttttttg catattttta
       61 gcatttattt cctaaaaaat gtaaggggat tcctatattt ttaagtatac ttgtttttcc
      121 ttttatacat tttatggtat tatcatttta ttagttatta taaaacaatc gttagaaagg
      181 acgtcattat gcaagtatac ggaattgatg cggtgcttcg aatcgtgagt catttggcgt
      241 ttatctactt agccttctgg tctttacgtt cgttacgcat tgaaactttt ttcagaccaa
      301 tgcataatac gcaaattaga atggcaatcg tacttttttc aattttttta gggtacactg
      361 caagtaattt tttcttagaa gttatcgctt tatgccgaaa tcttttcgtt agcctgtaaa
      421 ttttaaaaaa aggtactatt ttaaaagcaa ggatgttata atagaaaaga ttttgagtaa
      481 gtgtatgctc attggaattt tttggaggga acaacatgga agagatcatc gtaagaggtg
      541 gcaatcaatt aaatggaaca gtaagaattg aaggagctaa aaatgctgtt ttgcctattt
      601 tagctgctag tttgttagca gaagaaggta ttacgacatt agacaacgtt ccgattcttt
      661 ctgatgtctt cactatgaat caagtgattc gtcacttaaa cgtagatgta gattttgatg
      721 aacaaaagaa tcaagtaaca atcgacgctt caagacaatt agaaatcgaa gcgccttatg
      781 agtatgttag tcaaatgcgt gcttcaatcg ttgtgatggg tccgctactt gccagaaatg
      841 gacatgcaaa agtagctatg cctggtggat gtgcgattgg taaacgacca atcgatttgc
      901 atttaaaagg atttcaagct ttaggagcaa aaattattca aaaaaacggc tatatcgaag
      961 caattgcgga tgaattgatc ggtaacacga tttatttaga tttccctagt gtaggtgcta
     1021 ctcaaaatat catgatggct gccgtaaaag caaaaggaac aaccatcatt gaaaacgtag
     1081 ctcgcgaacc agaaatcgtt gatttagcaa acattttgaa taaaatgggc gctcaagtct
     1141 atggagctgg tactgaaaca atgcgtattg aaggcgtgga tcatttacat gcagtcaacc
     1201 actcaatcgt acaagatcgt atcgaagcag gtacctttat ggttgctgct gcaatgacac
     1261 aaggcaatgt gctgattgca gatgctatct ctgaacataa ccgcccattg atttctaaat
     1321 tgatcgaaat gggtgcagaa atcattgaag aagaaggcgg agtccgtgtc atcggaccaa
     1381 aacatatctt acctacagat gtaaaaacga tgcctcatcc tggtttccct acagatatgc
     1441 aagctcaaat gacagctatc caattagttg cagaaggtac aagtgtagtg actgaaacag
     1501 tgtttgaaaa tcgtttccaa catttagaag agatgcgccg tatgaacgct catgtcaaaa
     1561 tcgacggaaa tgtagcaatc atggatggaa accatgaatt gcaaggtgca gaagtatatg
     1621 caacagactt gcgggcagca gcagcattag ttttagctgg cttgaaagca aatggaatca
     1681 cacgtgtgcg taacttgaat tatctagacc gtggatatta taatttccat atcaaactac
     1741 agcaattagg tgcagacgtt gaacgtgtag acatggatca aacttctgct gaaaaaacag
     1801 cacaaacgat tgcttaagga agtggctgaa tgagtaacca acgatatatc ttattaactt
     1861 tgataaagat tttaatggtg atcttattgc ttatccttct ttttgtggcc ggaacgatga
     1921 tcggatatgg tgtcatcggt ggcggcaatc catttaaagt atttcagcca agtttgtgga
     1981 ttcatattcg agactttttt cattgataaa taagaatcac agtggttcta gtcgatatga
     2041 agtgagccat gaagcagaac atgataaaag aaaacagaag ggaacgttga cgttagtcgc
     2101 gatccctttt tttagtaaaa gaaaagaagg aatctaattg atgaagaaga tattgattta
     2161 tttagttaga ggatatcagc gctttatttc gcctgcattt ccacctagct gccgttatta
     2221 cccaacctgt tccaattata tgataaaagc aattcaaacc catggcgcgc taaaagggac
     2281 ggtaatgggg actgcacgga tcttacgctg tcatcctttt gtcaaaggcg gtatcgatta
     2341 tgtaccattg acattccgat taacgcgaaa taagaaagat aaaaaagaac cagattataa
     2401 aaaggttgtg aaaaatacgg gtagcactga gcgataaaga aacaatttgg aaaatagctt
     2461 cttatatttt gcaaaattgt gagttattgc cgaagtgttg gattttgaac accgtcaaaa
     2521 aaaaggttgt gaaaaatacg gctagcactg agcgataaag aaaaagagga aatttattgt
     2581 caaagtaaca actccgcaac tacttatatg ggggacaaaa aatgatcgaa gaactaaaca
     2641 agaaagattt accatggtcc ttgttattag atgcagatcc tgataaagaa aaagttcaag
     2701 tatatgtttc cagagggagt ggctggatat ggaaagaaaa agaaaaaaca ataggtgtac
     2761 tgatctatgt ggcaagggaa aaagaatacg aaatcgtcaa tgtagcagta accccaacgt
     2821 atcaaggaaa ggggattggc ggaaaattac ttgagacagc ttttcagaag ctttcacaac
     2881 tcgcttccag tcaaactcag atcatcatac gaaccggcag tactagcagt gcggcgttac
     2941 atctctatca aaaaatgggc tatgtggaga ttggtcgagt caaagactat tttatccatc
     3001 attactctga gcccattttt gaaaatggag atcaattaag ggatcaagtg attttagcaa
     3061 ttcacttacc taaaaatagc tttaactaat aaaatttcaa ttcaagaaaa tgctttcaaa
     3121 tgctaaatag atacattaca atagctttag caaacaaaaa ggagaatatt tttatgggaa
     3181 tattagtttt tgattttggt ggatcggctg tcaaatatgg ttgttgggat ggcaaagaga
     3241 ttaaaggtaa gggacaattt gcaacgcctg aatcttggga agaaatgaaa gcgcaattat
     3301 ttcaagttta taaaaaaata ctcgtttctt ttgaagtcga aggaattgga atcagctctc
     3361 ctggagtagt gaataatgag aaacaggtga ttgagggaat cagtgcgatt ccttatattc
     3421 atggatttaa tattttccga gatttggaaa ctctgtttca acttccagta acgattgaaa
     3481 atgacgccaa ctgtgccgga atggctgaat tttatgaagg tgctgcccgc gattatcaag
     3541 atgttgcttt tgttgtggca ggaactggga ttggcggggc gttgttcacg aaaggacaaa
     3601 tcaataaagg ggcacatctt tacggtggag aatttgggtt gatgttctta aatggcgata
     3661 agacattcag taaacttggt acagcggtac agatggcttg gcgttattgt gaacgcaaag
     3721 gtctcgataa aaatgcttat accggaaaag atgtctttga attagcagaa actggagatg
     3781 caatagcaaa agaagaagtt gagagttttt atacgtatct tacaaaggga ttattcagta
     3841 ttcaattttc gtttgatcca gaagtgatcg tattaggcgg cggggtttct gcaaaagaag
     3901 ggctcattga tgagatcaaa agccgaatga agaagctgac agaacaattc gatctgcatg
     3961 atttcgaacc gcagatttta ttatgtgaat acagaaacga tgctaatctt gtaggtgcag
     4021 cagcaaacta tctcgctgta cgtcaagggg agaattaaga gctttagaga aggtgcagct
     4081 tgcataaaag aagtgaatga aatgaatagg agatttttac ctaaggaatg ttttatttgc
     4141 tgcagtatga taaaatatct gagaggtgaa aatgatgtca aaaaaaaata aagatattga
     4201 agtgcgtata gaagaagtga aaaaagatat caaaggtaag aattatgaag tcactcagtt
     4261 atttataggg aaaaaaatga ttggtgagat cttagcctat ggaccaaaag aatatgaaat
     4321 tttctttggt gaagaagatt ttggaaaaga aaaaagtctt gaaaatgcga tagaaacagt
     4381 gattcgacac tggaatctgc acgaataaaa aaatatcaaa aaaactttca ataaagggtt
     4441 gcaaaaatct ggaaaattag gtattataac taagtcaggt tgatacaaac ctttcggagg
     4501 agtagcgaag tggctaaacg cgacggactg taaatccgtt ccttagggtt cagtggttcg
     4561 aatccactct cctccatttc attggggtat agccaagcgg taaggcaagg gactttgact
     4621 ccctcatgcg ttggttcgaa tccagctacc ccagtatacc acaatcgtaa gctctcgtta
     4681 gttcgagagc tctttttttt gctttgctac cttgttcctt gaagattctg ctgatttaag
     4741 atagagttaa gttaactaca agaattggag ggatccacat gaaatggaaa gaaaaagtag
     4801 acgaagcagc tgaaaagtta tatgatttgg tcaaatcaga aaaatacaat attgaagtga
     4861 atattccgaa aaaaggcgga aaagcggtcc gggtaaaatc aaaacgccca acgaatcata
     4921 caaaaaaatg gatggctaaa aaccgctaat aataacaaaa gatccataag tcggcatttt
     4981 catcgatttt agggtctttt gttattttct aggttggaca tatctaaaaa attgtttctg
     5041 acaataggag taggaagaac gatgtgtgtc taaatcctct gttgatggat agaaaccttt
     5101 atctttttca gttgtgaatc aggtatatac cagttataat agaagaaacg aaaggacggg
     5161 tgtacgttat gtcgaatcaa ttacctgtat atatacagat tcatgaccaa ttaaaaaatg
     5221 aaatcgaaca aggtatatgg aaagtcggag accgtcttcc ttcagaaaga gaattagcag
     5281 tgaaattcaa tgtcagccgt atgacgttga gacaagcaat tcaaaacttg gcagatgaag
     5341 gaatcttaga aagaaagatc ggttcgggta catatgttgc acgagaaaaa gtacaagaaa
     5401 aaatgtctgg tgcaacaagt tttacagaaa ttatggaatc acaaaatcga gtaccgtcta
     5461 gtcggacaat ctcttatttc ttgacttcgc caagctcaag tgaaatggaa aaactaaact
     5521 tgagtaaaga tgacacgatc ttgcgaatgg aacgtattcg tttcgcagat gatgtcccta
     5581 tttgttttga agttgccagt gtgccacaaa agctgatcga tggctacagc aaagcagaga
     5641 ttactgaatc gttgtatcgc acgctagaag aaaaaggcgg gaaaaagatc ggtccagcga
     5701 atcaaacgat tactgctgtg ttggcttctg agaaaattgc tgactattta gaggtaaaac
     5761 gaggagatgc agttcttcgt ttgcgccaga tttcttattt tgaagacggt acgccgtttg
     5821 agtatgtgcg tacgcaatac gtaggaaatc gttttgagtt ttatttggaa aaataaaaaa
     5881 caggtgatcc ctgattccgt attagaaata aaagagaatc agaatcacct gtttttatat
     5941 atgatcagtt accagttgct cgtgataaga aacaggaccc tcttcaaatt ggacttgtcc
     6001 gttcagcagc tcaatgatgg attctttaaa gtggtcagca cttttttcat ctacagcaca
     6061 gatgacgcta acttgatcag tatattggat ttcttttata aaaaaatgtt ggagttcaag
     6121 ataattctgt aattttccta gttgcggata cgtgatcatc acagagatct cctgttgcaa
     6181 ggtaccttct acgattccaa tgtcagacaa tgcttgagag accgcatgag tgtatgctcg
     6241 tattaatccg cctgtgccga gtttcgttcc tccaaaatat cgagtgacta ctgcagctac
     6301 attgatcagt tgattttttt tgaggacttc caacatcggt atgcccgccg ttccacttgg
     6361 ttctccatca tcgctgcttc gctggatttc atttttttct ccaatgatga aggcgctgca
     6421 gttatggttc gctttccaat gttctttttt tatttcttga atgaaacttt ttgcttcgtc
     6481 ctctgattct attcgtctaa gagaacagat aaagcgtgat tttttgatct cgatctcact
     6541 ttgtccattt tcttttattg tacgatagcg ctcgatcatc ttgtcacctc ttttttcatt
     6601 ttctttattc agtataaata aagttctagg ttgtttcaac actgtaacgg ttgcgcaatt
     6661 gctgtagtat gcgataataa agacaaaggc aggtggatct ggggatgcag gaacgaatga
     6721 aaaagtacga tgcaattacg cattatttga agaacaatgg cggctctcaa gtaacgttga
     6781 cctttaccca atttgacgaa ctgctgtttc ccagtaacgg acttcccaaa acagcaaggg
     6841 aaagtacaga ttggtgggca aatgattata aacaccctga aaaaggtgcc tatggctgga
     6901 taaatgcagg ctatgaagta gttgtcatca atttggacaa agaatatgtc gtgttcaata
     6961 aattagtgaa atctagttgg ctatttgatt aaataaaccg cgtttagaaa acgttgacaa
     7021 atttctttct ttttatataa tagagtaaca gacgcgaaga aaacgtttgc gtagtttgtt
     7081 ttaaaaagga gagaaaagta tggctaaaaa aacaatcatg cttgtatgtt cggctggtat
     7141 gagcacaagt ttactagtaa caaaaatgca aaaagctgct gaagcaaaag gaatcgactc
     7201 tgatattttc gctgtgtccg cttctgacgc agataacaat cttgcaaata aagacgtgga
     7261 tgtgttgtta ttaggcccac aagttcgctt tatgaaagct gattttgaga aacgtctgga
     7321 accaaaaggc attcctttag atgtcatcaa tatggctgat tatggtatga tgaatggcga
     7381 aaaagtgtta gatcaagcaa tcacgctcat aggtgaataa atagaacagt ataactatcg
     7441 aacaagcgat ccaaaaaata gatcgcttgt tttttcgtat atttttttag ggcgaataat
     7501 atgaatcatt tttggggtag aaggcttctg acgagagata ttaaagcaat agactgtgag
     7561 gaggtaaatt taccaaatta tgagaagatc gcagcagtgg aacgtttgag caactggatt
     7621 ctttgtcatc gctgtggaat gagaacgtcc gtcattgatg gacagcttgc agattacggt
     7681 tatttttgta cacattgtct atatttaggg cgatgcgaca ctcaacaaga tttatatttg
     7741 tttgatcagc ctgaagcaaa acctcgagaa gtcgtctttt tttggacagg acagcttact
     7801 gaaaaacaaa cggaaatctc aaagagaata ctttgccatt cagaaaaaag acatcatctt
     7861 atttgggctg taacaggtgc agggaaaaca gagatgcttt atcctatttt agtggaaact
     7921 ttaaaagctg gaggaagagt agcgatttgt acaccgcgag tcgatgtttg taatgaattg
     7981 tttttacgct atcgttatgt ttttccagaa gaaaaaatca cgttgctcca tgggaataca
     8041 gcaacggctt atacctttac ttcttttgtt atctgtacga ttcatcagct ataccgtttt
     8101 tatcggtcct ttgatttaat agtgatcgat gagatcgatg cgtttcctta taacggggat
     8161 gcaggattag ctcatgctgt gcaaacagcg attaaaccag acggacgatt catctactta
     8221 agtgccacac ctgataaaaa gttactaaag gagatcaagc agacgtttga catccacaaa
     8281 cttgctttac gctttcatcg gcgtttgctt cctgaacccg tcttggtttt catgaataat
     8341 tggcgacaaa aatgcaagaa caagaagaag ataaaatctt tggtgcggat cattcatcaa
     8401 ttaattaaag aaaaccatat ccttttattt tgtccaagta tcacgatgat gcatcgtttg
     8461 aaagccacac ttcaagaggc tcttccgaga tatgagatca cagatgtttc ttcaagagac
     8521 aatcagcggg cggaaaaagt ccagaaaatg cgagaagcaa aatatgacat actgttaacc
     8581 acgatgatcc ttgaacgtgg agtcacattt gaaagaatat ccgttatcgt tttaggagcg
     8641 gatcatccgg tttttactaa atcttctttg gtacaaatcg caggaagagc cgatcgtaaa
     8701 gggccttata cgaacagtca agtatatttt ttctatgaag aaaaaactgc agctattcga
     8761 aaagcttgtc aggaaatcaa agaaatgaat gaagaaggga agaagatact gtgaggtgca
     8821 gttgctgcag taaagatatc gtccaaaatt taacattcag cgagattatt ggtgtttcga
     8881 aatacagatt acctcagact tgttcagttt gtcgcgcaca atttcattta ttggaaacaa
     8941 gaggctgttc tggctgtcaa aaaccaatcc atttcaatct agagagtacg aaaaagaaag
     9001 taaattctat ccccgtttac tgcagtgact gtcaagaatg gaagaagaaa tatccgacct
     9061 atgattttca gcaccgtgcc ttttttgtgt acgatgaaaa tatgaaggcc tggctcaatc
     9121 aatacaagtt tatgggagat atccgactag caggcacctt tgcatataaa tggacgcaga
     9181 taaaaaaaga atacccgaca tacatctatt gtccaatacc tttatctgaa aaaaggcgaa
     9241 cagaaagagg attcaatcaa gtaagcgaga tgttgaaagc agcaagaatt ccttttcaga
     9301 tgctactcaa aagagagaag catctgctgg ctcaagcaaa aaacacgcga aaggaaagat
     9361 tggagatgcc tcaacctttt gaacttgcag tcaccttttc taagatacaa aataaaaaaa
     9421 tattgattat tgatgatgtc tatacaacag gacagactct tttccacgca gcagattgtt
     9481 tgcagatcgc acagccgaaa atcatccgaa ctatttcttt ggcgagatga attagatgtg
     9541 gaaatttgat agcgatttaa tttgcaaata acctagagtt cgatataata gaattaagaa
     9601 gagggaaaga gtggtccttc tccttcttga ttgtggtaga cgaaaggggt aatttttatg
     9661 tttagatata atgtacgcgg cgaaaatatc gaagtaactg aagcgattcg cgactacgtt
     9721 gagaaaaaag taggcaaact agaacgctat ttcagtgacg cacccgatgc aacggcgcat
     9781 gtgaacttaa aagtttatac agaaaaaaca gcaaaagtgg aagtaacgat cccattacct
     9841 tatcttgtat tacgtgccga agaaacttct ccagatttat acgcaagtat cgatttggtt
     9901 gttgacaaat tggaacgcca aatccgcaaa ttcaaaacaa aaattaatcg taaatcccgt
     9961 gaaacgggca tgaatactgc aaatgctgct gctttattca atgaagagga cgaaaacgaa
    10021 aatgattctg aattagatat cgtacgtacg aaacgccttt cattgaaacc aatggatagt
    10081 gaagaagcag tcttgcaaat gaacatgctg ggacataact tctttatttt tgaagatgca
    10141 gaaacaaatg gtacaagtat cgtttaccgc cgtaaagatg gaaaatacgg actgattgaa
    10201 acagactgat cagccttact taaataacaa cgaaaacttg cagcagctac tgacagctgc
    10261 cgcaagtttt tttgagtttc ctcctctttt agatgaaata tgaataaatt atgaagttct
    10321 tactttaata cgcttaagat atttcaatta tctataacta atgataaaat gaaaagtaaa
    10381 gtctacatag tctggataat acacagaagg aaaaggagat tacacaacgc atggccaatt
    10441 tgattcgttc acttatcgaa aacgataaga aagaactaaa acgtttagat agcattgcaa
    10501 aaaaagttga agcatacgct gaccaaatgg cagcattaac tgatgaacaa ttacaggcaa
    10561 aaactgctga attcaagggc agataccaaa aaggagaaac attagatcaa ttacttccgg
    10621 aagcttttgc agtcgtccga gaagcagcaa aacgtgtatt aggtttatat ccataccacg
    10681 tacaattgat gggtggaatc gtgcttcacg atggaaatat ccctgaaatg cgtactggtg
    10741 aaggtaaaac cttgaccgca acgatgccag tatatctaaa tgctctgact ggcgaagggg
    10801 tacacgtagt taccgtcaat gaatatttag caacccgtga ctcgacggaa atgggcgaac
    10861 tttataattt cttaggatta acagtggggt tgaatatcaa ctcaaaatct tcggatgaaa
    10921 aacgcgaggc ttataattgt gatatcactt atagtacgaa taatgaatta ggatttgact
    10981 acttgagaga caatatggtc gtttaccgcc atcaaatggt acaacgtccg ctgaattatg
    11041 ccattgtcga tgaagttgac tcaatcttga ttgatgaagc gagaacgccg ctgatcattt
    11101 cagggcaagc agaaaaatcc acagctttat atacaagagc agacaacttc gtgaaacgtt
    11161 tgaaagaaga agaagattat aaaatcgatg tacaatcaaa aacaatcagt ttaaccgaag
    11221 cagggatcga aaaagcagaa gaaacattcg gtttggataa cttatacgac attgaaaata
    11281 ctgcattgac ccatcacttg gatcaagcat tgagagccaa ctatatcatg cttttagata
    11341 tcgattatgt tgtccaagac ggaaaagtac tgattgttga ccagttcact ggtcggatca
    11401 tggatggacg ccgttattca gatggacttc accaagcaat cgaagctaaa gaaggcgtag
    11461 agatcgaaga cgaaacgaaa accatggcga cgatcacttt ccagaactat ttccgtatgt
    11521 acaagaaact tgcgggaatg actggtacag ctaaaacaga agaagaagaa ttcagagaaa
    11581 tttataacat ccaagtatac cagattccga caaatcgtcc ggttatccgt gatgatcgtc
    11641 cagatttgct ttacccaaca ttacaaagca agttccacgc tgtcgtgcaa gatatcaaag
    11701 atcgctaccg caaaggtcaa cctgtgttag tcggaactgt tgcggttgaa acatctgagt
    11761 tattatcaga tatgctgaat caagaacgta ttcctcatga agtgctgaat gcgaaaaatc
    11821 actttaaaga agcagagatc atcatgaatg ctggtcaaaa aggtgctgtg acgattgcta
    11881 ccaacatggc tggtcgggga acagatatca agttaggact tggggtaaga gaacttggtg
    11941 gtctagcagt tatcgggaca gaacgacatg aatctcgtcg tatcgataac cagcttcgag
    12001 gacgtgcagg acgtcaagga gaccctggta tgtcacagtt ctacttgtca ttggaagatg
    12061 atttgatgaa acgattcggt tcagaacgaa tcaaatcttt cttagatcgg atgaagatcg
    12121 gtgaggaaga tgctgttatc caaagcaaga tgctgacgaa acaagtagaa tctgcacaaa
    12181 aacgtgtaga aggaaataac tatgacacac gtaaaaacgt cttacaatac gatgatgtga
    12241 tgcgtgaaca acgtgaagta atctataaac agcgacaaga agtaatcatg gaagaaaaaa
    12301 gcttatccaa gccactaatg aacatggtca aacggacaat cagtcgtgta gtagatgcgc
    12361 atacacaatt agagaaagaa aattggaatt tggaaagtat catcgatttt gccggcagta
    12421 ctcttgtcca tgaagatagt attactcttg cagatatcga aggaaaaaca gctcaggaga
    12481 tcaaagagta tctaaacgaa cgagcacaag aagtatttaa taccaaagcg gctcaactaa
    12541 atggtccaga gcaactgttg gaatttgaaa aagtcgtgat cttgcgtgtt gtggactcta
    12601 aatggacaga ccatattgat gcaatggatc aattacgtca gtcaatcggc ttacgtgctt
    12661 atggacagaa caatccatta gtcgaatatc aaacagaagg ctataagatg tttgaagata
    12721 tggtaggcgc catcgagtat gaagtcactc gaatctttat gaaagctgag atccgtcaaa
    12781 acgtacagcg tgaacaggtt tctcaaggaa cagcttctca ctcagaagac ggagatgcca
    12841 aagaaaatag taatactagc gcgaaaaaac agccaatcca tgtgaacaag gtcggaagaa
    12901 atgatccttg tccttgtgga agcgggaaaa aatataagaa ctgtcatgga aaaaatgcaa
    12961 actaatttct gacagcagga ttggtgagac aaattctttt tttaagaatt cgtctcacct
    13021 tctttctgtt atttgctgat tctgttagac tagatataat gaaaaaagta tagtgaaaga
    13081 aggaaaaaac atggaaatat ccgaaattcg aaatctcctg acagaaatgc aggaaaaggt
    13141 cacaagcttc aggaggtctc tttgacttag accagctgga agaagacatt gcggaagcag
    13201 aagcaaaaat ggctgaacct ggtttttggg atgatagcga agctgctcaa aaagtgatca
    13261 gtgaaaacaa tgcaaataaa gaaacttatg atcaattcca ttctcttgct gaagaactgg
    13321 aagaaatcga attgatggtg gagatgttgc aggaagaacc tgatgcagcc atgcaagaag
    13381 aagcagaaac acgtatcaat gcgctagatg aaaaaatgaa gacatatgaa ttatctatgt
    13441 tgttagatgg tccttatgat cgaaacaacg ccatcgttga attgcatcca ggggctggcg
    13501 gaacggagtc ccaagattgg ggcagtatgc ttttacgtat gtatacacgc tgggcagagc
    13561 agcatgggta tcaagtagag acactagatt atcaagcagg tgatgaagca ggaatcaaga
    13621 gtgtgaccct actgatcaaa ggacacaacg catttggtta tctgaaatca gaaaaaggtg
    13681 ttcatcgcct tgtacgtatc tcgccttttg actcagcgaa acgacgtcat acttcttttt
    13741 gttcggtaga tgtgatgcca gagcttgatg aaacgatcga tatagaaatc aatccagatg
    13801 atttgaaaat cgatacgtac cgagcaagtg gtgcaggtgg tcagcacatc aataaaacag
    13861 aatctgctgt acggatcact cacttgccta caggaacagt agtagctagt caggcacaac
    13921 gttcacaatt gaaaaaccgt gagcaagcga tggggatgtt aaaagctaag ctttatcaat
    13981 tagaagtgga aaagaaagaa caagaagcag ctgcacttcg aggagaacaa aaagaaatcg
    14041 gctggggttc acagatccgt tcttatgttt tccatccata ttctatggta aaagaccatc
    14101 ggacaaacgt agagacagga aatgtccaag cagttatgga cggtgacctt gatttattta
    14161 ttgacggcta tttaaaaatg catctacaat aagaacccat tgttaaattg aaataaaatt
    14221 gtaagatttt gcaagcatgt tgaaacattg ccatgctata atagctaaag actgaaaaag
    14281 atatggagag aacgccatga ttgaaatgaa ggatgtaatg aagaagtact caaatggtac
    14341 gaccgctatc cgcaatctct caattgtcat cgaacaagga gagtttgtct atgtggttgg
    14401 tccgtctgga gccggtaaat ctacattcat caaattgatg tatcgagaag aaaaagcttc
    14461 aaaaggaact ttgaacgtcg caggtcatga tctaatagca atcaaaaacc gcgaagttcc
    14521 ttatctacgc agagaaatcg gcgtggtgtt ccaagactat aaattattac caagaaaaac
    14581 agtatatgaa aatgttgcgt acgctatgca agttatcggc agaaaacctc gggatatcaa
    14641 aaaacgagta atggaagtgc tggaccttgt tggtttgaaa cacaaagttc gagtctttcc
    14701 tagcgaatta tccggtgggg aacagcaacg tgtctctatt gctcgggcaa tagtgaatac
    14761 gcctaaagta cttatcgcgg atgaaccgac aggaaatctg gatccagaaa actcatggga
    14821 aatcatgaaa ttattagatc ggatcaatgc ccaaggaact actgtcgtga tggctacaca
    14881 taacagtacg atcgtaaata cgattcggca ccgagtaatt gctatcgaaa acggccgcat
    14941 tattcgagat caagcggaag gggaatatgg atacgatgat tagaaccttt ttccgccatt
    15001 tattagaaag tatcaagagt ttgaaacgta acggctggat gacagttgct tcagtcagtg
    15061 cggtaacgat cacattgact ttagtcggtg tattcatggc agttatcatg aacgcaacta
    15121 aattagctca agatatagaa ggaaacgttg atgtctctgt attcgtagat atcggtacaa
    15181 aagacgaaga gatgaaaaag ctagaaaaag aattgaatga cttagatcat gttaaaaaag
    15241 ttgaattttc aagcaaagaa caggaattga aaaaaatcca aaacgaaatg ggtgattcct
    15301 ggaaattgtt tgaaggtgac aataacccgt tatatgatgt gtatgtagta agtgctactg
    15361 atccttctta tacaaaacaa gtagcagaag aagcagcaga tttgaagaat gtttctcgtt
    15421 ctgattacgg tggagcttcc tcagatcgga ttttccaaat cgctggtgca gttcggacat
    15481 ggggattagt tgcagcggca ttgctgctgt tcgtagcgat gtttctcatt tcgaacacga
    15541 ttcggatcac gattttgtct agacagcgtg aaattcaaat catgcgttta gtcggtgcaa
    15601 aaaatggata tattcgctgg ccgtttttcc ttgaaggtgg ctggatcggt ctgatcggtg
    15661 cgattcttcc gattttgatc atcacatttg gttatgcatg gtttttccaa ttgatcaatc
    15721 catcattatt acgttcccat tattcattga ttcatccaca aaatatcgtt tggaaaatca
    15781 atcttttaat ggtcggcatt ggtgtgatca ttggttcact tggctcgatc atttctatga
    15841 gacgtttctt gaaattctaa taccacaata taaataaaat gagaaagttg gacagtttcg
    15901 ttccagcttt ctttttttat tggctaggag aatagaagga agcggctttt ttccctttac
    15961 tttaaaaaac aacttgggta ttcttaagtt ggtagaagaa gaggtgaagc cgtgaaaatt
    16021 atcgttgaca atttttttac gatccttttg attatgaata ttttgctttc ctttatcatc
    16081 gtttttcgag aaagaaagga aactgctcaa acatgggcat gggtgctcgt tttgatgttc
    16141 attccagttg taggatttat tttatatatt tttttaggaa gagggatttc caaagagaaa
    16201 atctttgatt tgaagatcca agggaaaatc ggtaagaacc tggaaattga agaagacaaa
    16261 caggcattga tgcgagggct ttatcctcat ccgcctactg gacaagtaga tgtgaaacaa
    16321 ttgatctata tgctcacggt atttgaaagt acgctctata cgactaaaaa tgaaatcact
    16381 ttatttacag atggtcgtga aaaattcgat gcgctgattg aagatatcaa gcaggcagaa
    16441 gaccatatcc acttccagta ttatatttat cgcagtgatg cattaggtga agaagttcga
    16501 gatgcattga cagaggcggc aagacgaggg gtaaaagtca gagtccttct tgatgcatgg
    16561 ggttccacac aagtttcttc cagctttttt cagaatttga aaaaagcagg cggagaagtc
    16621 gcatttttct ttcctctatt tgttccgtat attaatcctc ggatcaatta tcgcaatcat
    16681 cgtaaaatcg tagttattga tgggaaaatc ggatatacag gtgggttcaa tgttggaaat
    16741 gaatatctag gattggtaaa gaaatttggt tattggcgag acaatcattt gcgtatttac
    16801 ggagaagcgg tgtatacctt gcaaaaccgt tttttgatgg actggaattc tcagcataca
    16861 aaagaagtag gctattctcc caaatttttt ccgtccattc actctactgg tgatatagct
    16921 gttcaaatcg tgacaagcgg accagatacc gaacatgaac aaatcaaaat gacttatttg
    16981 aaaatgattt cgttagccaa acgggagata ttgatccaaa cgccttatta cataccggat
    17041 ggatcgattc atgaagcact gaagttggct cttctttcag gcgtaaaagt tcatatccaa
    17101 atacctaaca aaccagatca tcttctcgtt tactgggcaa cgtattcttt cgctgcagag
    17161 ctaatcgagt atggagcaag gatcgaaaca tatgagaatg gatttattca tgcaaagacg
    17221 atgattatcg atggtggcat tgtttcagtt ggctcagcaa atatcgatgt tcgttcattt
    17281 cgcttggact ttgaggtcaa tacactggtt tatgatgaac gaatggcttc acgtgtaaga
    17341 aaagcttttt ttgaagactc gaaaatttct acgcatctca caaaagaaat gtatgaaaac
    17401 cgaggaataa tcatcaagat gaaagaagga ttagcaagat tgatttcgcc gctcctttaa
    17461 aacataaaaa ggacatgaga accatcaaca gatggagatc tcgtgtcctt ttctaacggg
    17521 gccatttctg agagtacgtc agaaatggag aaagcgtctc gcatgagcga aattaacttt
    17581 ggggtatctc ttgttttttg gggaaagcaa gaaataagct aatatatgct attaaaagag
    17641 acacatttca aagccagtat agcatagaaa cttttaatga gatagttttt
//
LOCUS       DAZBTF010000058        16998 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000058 DAZBTF010000000
VERSION     DAZBTF010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.058"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..273)
                     /locus_tag="ACHGNS_002034"
     CDS             complement(<1..273)
                     /locus_tag="ACHGNS_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFN6937962.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGING"
     gene            complement(275..472)
                     /locus_tag="ACHGNS_002035"
     CDS             complement(275..472)
                     /locus_tag="ACHGNS_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFN6937963.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(866..2593)
                     /locus_tag="ACHGNS_002036"
     CDS             complement(866..2593)
                     /locus_tag="ACHGNS_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFN6937964.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2586..3779)
                     /locus_tag="ACHGNS_002037"
     CDS             complement(2586..3779)
                     /locus_tag="ACHGNS_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFN6937965.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3966..4610)
                     /locus_tag="ACHGNS_002038"
     CDS             complement(3966..4610)
                     /locus_tag="ACHGNS_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN6937966.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4704..4838)
                     /locus_tag="ACHGNS_002039"
     CDS             complement(4704..4838)
                     /locus_tag="ACHGNS_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFN6937967.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4840..6972)
                     /gene="feoB"
                     /locus_tag="ACHGNS_002040"
     CDS             complement(4840..6972)
                     /gene="feoB"
                     /locus_tag="ACHGNS_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFN6937968.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6969..7442)
                     /locus_tag="ACHGNS_002041"
     CDS             complement(6969..7442)
                     /locus_tag="ACHGNS_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFN6937969.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7843..8067
                     /gene="nrdH"
                     /locus_tag="ACHGNS_002042"
     CDS             7843..8067
                     /gene="nrdH"
                     /locus_tag="ACHGNS_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFN6937970.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8226..10385
                     /gene="nrdE"
                     /locus_tag="ACHGNS_002043"
     CDS             8226..10385
                     /gene="nrdE"
                     /locus_tag="ACHGNS_002043"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFN6937971.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10435..11400
                     /gene="nrdF"
                     /locus_tag="ACHGNS_002044"
     CDS             10435..11400
                     /gene="nrdF"
                     /locus_tag="ACHGNS_002044"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFN6937972.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11489..12628)
                     /locus_tag="ACHGNS_002045"
     CDS             complement(11489..12628)
                     /locus_tag="ACHGNS_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFN6937973.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12937..13650)
                     /locus_tag="ACHGNS_002046"
     CDS             complement(12937..13650)
                     /locus_tag="ACHGNS_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFN6937974.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13904..14605)
                     /gene="nagB"
                     /locus_tag="ACHGNS_002047"
     CDS             complement(13904..14605)
                     /gene="nagB"
                     /locus_tag="ACHGNS_002047"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFN6937975.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14839..16371
                     /locus_tag="ACHGNS_002048"
     CDS             14839..16371
                     /locus_tag="ACHGNS_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFN6937976.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16562..16741)
                     /locus_tag="ACHGNS_002049"
                     /pseudo
     CDS             complement(16562..16741)
                     /locus_tag="ACHGNS_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(16761..>16998)
                     /locus_tag="ACHGNS_002050"
     CDS             complement(16761..>16998)
                     /locus_tag="ACHGNS_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN6937977.1"
                     /translation="PRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVK
                     GLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         5270 a         3495 c         2926 g         5307 t
ORIGIN      
        1 accatttatt ccagcagttc ttccaatttc actcaaattt cttattaatt cctcttgata
       61 ttcagtgaat cttattttat tagtataagc agtaaaatat agataattca ccaatctatt
      121 tattaaagga ctgggatcta attcatattt ttgtttttct aattttaaat ataccttttg
      181 taaaacctca agaatatcag aatatttagg atctttactt atagatatat cattatataa
      241 ttcatgaaca atttgcttag catttttttt cattttagca cttccctgga attgctccac
      301 ctaaaaaacc acctatagac attcctgcaa tacaagtagt tgccttggcc caatcgaccg
      361 tacatttatt tttagtgcaa tacactccat ttccataata ttttccacta tgagtggtac
      421 ccccatagat aagttgtgtc tctttaatag acaaaatttt taaatgtttc ataatctaat
      481 cacccctaaa ttaatgtttt tagactatta ttctaacatt cattaaattt caaaaaacaa
      541 ttccttcttg aaacgtagca aaataattcc tgaataagaa tttaatcact tttaagtata
      601 taaactatgt atattcttta atattttttg agaatgcact tcgtacataa taaactttaa
      661 tcacattagt ctttcatacc tacgatcgat ctctttgact cgcacccata aattcataat
      721 agtttgtcgg cgtctttgaa cgtttcgaag atgagctacg cattctcttt ttttatagac
      781 aaaaaagata gagacgaaca tggagcttcc aaatttggat atttgatcca attttggaag
      841 ctccatttat ttcgtgtctg tctttttatt ttttccagct ttcgactaat tcttggttct
      901 ctttgatcca gtcttttgct gcttgctgtg gatcttttcc attgttgatt tccaacataa
      961 ctttttccat atctttttct gaccaatgga aattgtctaa tactttgtaa gcttctggct
     1021 ggtcttcttt caatcctttt cgaaccatcg tatggatctg ctcttcgctt cccattccgt
     1081 tttcaggatc ttctaagtat ttcagatcat atttggcaaa catccaatga ggtgtccatc
     1141 ctgtaactac aattggttcg tgttttttga tcgcttgacc tagtgcaacg gtcattgctc
     1201 ctgaagaaga tgtctctact ttccagtctt tcaagttgtc atatttttgg atcgtgtttt
     1261 cagcagccgt aactacacca gctcctggct cgattcctat gattttctta cctgcctgat
     1321 cggttaactc atcgatcgaa tttacatcca tgtaggctgg cactgctaaa ccaacttttg
     1381 ctcctgtcaa attggcacct aggtcttcga cttgatcttt atattgtgca tattgtgaac
     1441 catgagtttt aggtagccaa gctgaaacca tggcatctga ctcgccattt gaaacagatt
     1501 tccacatgat tgaattatct aatggcgtca tcgttacatc atagcccatt tgtttcaata
     1561 cttcaccgac tacatttgtt gaagcaactt ctgtatccca ttcaacataa gataagttga
     1621 tacgttttgt ttctttggca gatgaaaaac tccctatacc aatcgcacct aagatcacta
     1681 ctactacagc accaatgatc agtccttgac gttttttact tttctgtgtt gttgctcctg
     1741 cagctttttt attgtttggc tggttcaaat gctgtgtaaa acggtcaatg atgattgcta
     1801 agataactaa tgcaaccccg ttgacaaatc cattcccgac ttgtgctctt tgcaatgctg
     1861 ataatacgcc gcgtcctaag ccgggtgcac cgatcataga ggcaataacg accattgaaa
     1921 gtgacagcat tgttgtttga ttgatccctg ccatgatcgt acttttagct aaaggcaatt
     1981 ctagtttgaa tagtttttga cgacttgtgc tcccgaatga atcagaagct tctactaatt
     2041 ctttaggaac ttgtcgaatc cctaaattcg taaaacgaac ggttggtggc agagcaaaga
     2101 tgactgaggc aaatacccct ggaaccatcc cgataccgaa aaatgcaact gctggaatca
     2161 aataaacgaa tcctggcatt gtctgcataa agtcaaggat cggtttgata atactttggg
     2221 ctttactgct ttttgccatc aagatcccta atggtacccc aatgatgatc gagatcacac
     2281 tagataaaag aacaagtgtg acagtattca tcaaatcatt ccatagacct tggttataaa
     2341 taaaccatag accgattaat gtgaacaacg taagtcctgg acgtttctta gaaataaaga
     2401 aagctgctac cgtcaataac aagataaaca aaggagctgg aatcactaac agtaaatttg
     2461 taatccccga catcactgcc tgtccaatcg tttgtaaaaa tgaaaatagg ccagaaaaag
     2521 ttgtcgtaaa ccattctgtg atattttcta cccagtttgc cactggtaat tgatagttca
     2581 atagattatt catgttcact cacctcgctt gtttctgcta atgcttcaat cacactacct
     2641 cggatgacga caccttttaa ttttccatcc tctacaactg caagtggagt cggtgagtca
     2701 taaataatat tgaaaatatc acttaccacc atgtctttcc caatcaccgt cacattttca
     2761 tctaggaagt cgatcaatgg acgtttttgc ttcctagctt ctaacgcttg atctgcagtg
     2821 atgatccctt tcaggtgtct ctttctgtca acagccatca gcatgctgac ttcttcatta
     2881 cgcatccttg tcaatgcaac gtttggtcca tcagattcga tattcgtcgt caatgcaggt
     2941 accatgatgt tttgagccgt aagcactttg gaacgatcga cttcttccac aaattcacgt
     3001 acataatcat ttgctggatt ggtcaatatt tcttcacctg ttccgatctg catgatttcg
     3061 ccgtctttca tcaaggcgat tctgtcccca atccgtaaag cttcattcaa atcatgggta
     3121 atgaaaataa tcgttttttg aacattggct tgcaggtcca gtaattcgtc ttgcatttct
     3181 cgccggatca gcggatcaag agccgagaac gcttcgtcca tcagcaagat ctcaggatca
     3241 tttgctaaag ctctggctag gccaacccgt tgttgcatcc caccagataa ctgactagga
     3301 tattggtctt taaatgaaag caaactagaa ttttctaatg ctttttctgc tttttcttgt
     3361 cgttcttctt taggtacacc acggacttct aacccgtatt ctgtattttc caagatcgtt
     3421 cgatgtggaa acagaccgaa gttttggaaa accatattga ttttatgacg acggacttct
     3481 cgcaattctt ctttgcttaa cttcgataca tcttctccat caatatagat actgcccgaa
     3541 gttggctcaa tcagacgatt caatagacgg atcaatgtgg atttcccact cccggaaagc
     3601 cccatgatca cgaagatttc tccttctttt acgtcaaaat tcacgtcata cacaccaacc
     3661 gttgcaccgg ttttctttaa aatctccgtt ttcggctggt gatcattcat catatctaaa
     3721 gcggcttgtg tctttttacc aaatatcttt gttaaatgtt ttacttctac ttttaccaaa
     3781 caaattctcc ttttttatgt aaattcattt ttctttctga aaaaagtgac cacactatgt
     3841 taacatcatg tagtcacttt gtcaaaaatg aaagaaattt gcttgtccaa actgaaaaaa
     3901 acttggctaa atctttgtaa ataaaaggtt tagccaagtt aatatttttt taatatttca
     3961 cacaatcatt ctgcaagctg atgctccggg taaaaaaagt ttgtcatacg ctgtagtgta
     4021 tattcatttc caataaaatg aacgatgtcc cctgccgtaa atttagcata ggggccaggt
     4081 gaaacaagca gttcttcatt atgagaaatt gcgataactg tggcgccagt cgcctgccag
     4141 atattcaact cgctaattga acggtccaga tgcattgctt tttctgataa agataattca
     4201 aacggaagta aaggattcat cttatgtatt cgcttcgttt gattgaccag ctgatccaat
     4261 aattgagaaa aatgattcag ctcatcttgt tgcttctcca cacttgccat gatttctgct
     4321 tttgtatctt ggatggattg tacatcttga tattgcaata ggaaatcttt tgctttttcc
     4381 ttcgaagcaa caaatgcccc acttccatgc cgtacttcca tgattccaag atctaccagc
     4441 acattgatag ccttgcgggt agtctcagga gaagcattgt acgtactggc aagtgttgag
     4501 cgggcatgga tcttctcacc cactttatat cggttttcaa cgatacgttc ggctaaatca
     4561 acagcaatct gctggtagcg cgggcggacg atttttttct ttccagacat tttctttcct
     4621 ctttcttttt ttactttccc tcatcttact atgctctagc aaaataagca aaaaagggga
     4681 gaatagcctc ttgcaacgac tgatcatcgc ttgatctgtt ccttttttac tgggcaagct
     4741 gtttgacagt catcacaatt cttaccggat tttaaacgag tatagacaat tgcgcctgca
     4801 cttccaaaga ttaacacact taataagaat gtcgccatcc tattcctctc cttctaatga
     4861 caatatagta taaaactggt tcttggattt tggctgacgg aacaaaacat aaacgagtcc
     4921 tatcatcagt aaaatagcaa gatacgtccc taacgctacc tgtccaccct ccaataagtg
     4981 tcccaactga taaatcacaa aactaactaa ataagctaaa ccgcattgat agcctatggc
     5041 gatccaagtc catttcttgg cattcatttc ccgatggata gccccaattg ccgcaaaaca
     5101 aggcgcacaa agtaaattga aggctaacaa tgaataagct gctgcgggag tatatgtgtg
     5161 ttgtagcact tgccagattt ctctgccatt ttcggatact tccgcatgac caaacaaaat
     5221 accaaatgtg ccaataatat tttctttagc gaccaatcct gtaatcgctg cgactgttcc
     5281 ttgccagttt ccccaaccaa gcggctgaaa aacaactgcc aggattcgcc cgaaaaaggc
     5341 taagatactt tggtcttcag aaacaggttg agcataaaaa ttataagaag agctgaacca
     5401 gatcacgata ctagaaacaa agataatcgt tcctgctttt ttaacaaaag acttgctgcg
     5461 gtcataggtt tgtcttagga ccgctgacca ttgaggaaaa tgatatgcgg gcaattccat
     5521 gataaaagga gctgtatctc cagcaaacaa acgtgttttt ttcaagctga ttccagagaa
     5581 aataattgca gtcataccaa gaaaataggc agaaggagct acccaagatt gatgaggaaa
     5641 aaaggcccct gctactaagg caataatcgg caactttgca gaacaaggca tgaaggttgt
     5701 gaccatgatc gtcaaacgac gatcttgctc gttttcaatc gtgcgactgg ccatgacgcc
     5761 tggcactcca catcctgtag caatcagcat aggaatgaag gattttcctg aaagaccgaa
     5821 tttcctgaaa agtcgatcca tgacaaaagc gatcctcgcc atatatccgc agtcttccag
     5881 aaagctcaag cataaaaaca atataatcaa ttgcggtaga aatcctaaca ctgctcccac
     5941 gcctgcgatg atgccgttca agatcagctc ctgcatccat cccgctactt gccattcagc
     6001 taacaatctg ccaacatgtt ctggtaacca tgtaccaaac aatgtgtcat tgatccaatc
     6061 tgtccccatt gttcctactg tttgaatagc cagataatag atcagccaca tcacaaatgc
     6121 gaagatcggc aaggctaacc aacgattcgt tgcaatctga tcgattttgt cactcatcga
     6181 taattggaaa gaatcatttg acacaacaca gagtgtacat agttgggtaa taaattcata
     6241 acgttcattg accagaatcg tctcactatc gtcgtccaat agtttctctg ttaactggat
     6301 cagttgctcg atctcttttt gctgagaaac tgctatctcg taatcctctc ttaccttctg
     6361 atctcgttca aaatatttca ttgcatacca gcgtttcttg ccgctttgtt ctcctagtac
     6421 atcttcaatt tcatgtaaag ctgcttctaa acgtggatca tatgtacagg gaacaggaat
     6481 agaaggccgc ttttttgcag actcctgaat cttttcgatt agtttttcga ttccttgctt
     6541 tttcaatacg ctgattggta aaactggtac accaagacca taggaaagct tttctagatt
     6601 gatttttttt ccttcttttt tgatgacatc catcatattc agaccaatga tcataggaat
     6661 gtctaaatcc aataactgcg tagttaaata aagatttcgt tctaaattgg tagcatcaat
     6721 aatgttgatg atcagttcag gctgttcctg aaccaaataa tctcttgcaa tcacttcttc
     6781 tggtgtataa ggagacaaag aatagatgcc gggtaggtcc tgaatatgaa tgttcggatt
     6841 ttttttcaac gatccttctt ttttttctac tgttacgccc ggccaattcc ctacatattg
     6901 attcgaacca gtcaagagat tgaacaagct agttttcccg ctgtttggat ttccgactaa
     6961 cgcgatattc attctttttc ctccctttgt atcaagatta gttcggcctc tgactttcgt
     7021 aacgtcagtt cataccctcg cactgtcagt tcgattggat ctcccagagg ggctagtttt
     7081 cggataacta tttttacatt tcgcgtcaat cccatatcca ttaaccgtcg cttcactgcg
     7141 ccttgtccgt ggattgctac gacgatccca gtctctcctg cttttaattg atcaagagaa
     7201 gtccagcttt cagaggaagc agtcttttta ttgacaaaga tttgacctag tatttcccga
     7261 cttactgcca ctcgatgact ttttaccagt aaaatggcat tgttgcctga taaattgacg
     7321 acgacgattt tacttccggc aacgatcccg atattttgca aatggcgttt tgttttttct
     7381 tctgtttcga ttcgttcgac ttgataaacg aaattctgct ttgcctgcgc taaggtctgc
     7441 atattgattc ctctcttgtg aaataaatca tgtattaaaa tcattcatta tatgttctaa
     7501 ttttcttacg tgttacgcaa ataaatgaga atcattctca catagaatac tctcggtaat
     7561 tgagaatgtc aattatactt gtgatattat gagcgaatct ttagtattga tagataaaaa
     7621 tgagaaaaaa atatttcttc atagttttcc acaatttttt ctctattaaa tttgctattt
     7681 tcccacacga tacttgtttt cagctctttt ttcaaaaaca caaaacttag tattccacag
     7741 tttccacaac acaatatgta gtgtttgttg tttgactttt ttactaagag ttttatacta
     7801 tctattatag ataataagaa tgtgttagag gaggcactca ccatgaacgt aaaaatcttt
     7861 tctaaaaata attgtatgca atgtaagatg gtcaaacggt ttttaacaga aaatcaaatt
     7921 gcttttgaag aagtcaatat cgatgaacag cctgaagcag tagactggtt gaaagcacaa
     7981 ggattccaaa gtgttcctgt cattacttct gatgcagaaa cagttatcgg attccgtcct
     8041 gatcagttgc gtaaacttgc cagctaacat gactaaaaga atcaaaacgt gatacatgga
     8101 tttgaagggc ttgagacagc accagttttt gtcgcaaact tccaaatcct tttttaaagc
     8161 ttaaaaacaa tgcaatgatt tgcaatcaag catcatcaaa tacgaaatga aggttgggcg
     8221 tacgtatgag tcttaaaaat ttacaagatg tcagttattt caagctgaat aatgaaatca
     8281 atcggccagt caatggtcaa atccctttga ataaggataa agaagcttta gttgcctttt
     8341 ttgaggaaaa tgtcaaacca aatacgatga cttttccatc tattatggaa aaaatcaatt
     8401 acttgatcga tcaagaatat atagaagcag aattcattca attgtattca ccatcattta
     8461 ttgaaaagct atatcaattt ttggacgagc aacatttttc tttcaaatct tttatggctg
     8521 cttacaaatt ttattctcaa tatgcgttga aaaccaacga cggttccgct tatctggaaa
     8581 cttatgaaga tcgagtagcc ttcaatgcgc tttactttgc gaacggggat gaagaattag
     8641 cacttgctct tgcagacgaa atgatccatc aacgatatca gccagctaca ccttctttct
     8701 tgaatgctgg acgtaaacgt cggggcgaat tggtgtcttg tttcttgact caagtcactg
     8761 acgatatgaa ctctatcggg cgctcaatca actctgcttt gcaattgtcc cgtattggtg
     8821 gcggcgtagg gattacttta tcaaatcttc gtgaagccgg cgctcctatc aaaggatatg
     8881 agggtgctgc aagtggtgtc gttccagtca tgaaattatt cgaagatagt ttcagctatt
     8941 ctaatcaatt aggtcagcgt caaggagctg gcgtggttta tttgaacgtt ttccatccag
     9001 acattattgc tttcctttca gcaaaaaaag aaaatgcaga tgaaaagatt cgcgtaaaaa
     9061 ctctttctct tggcgtgatc gtacctgata aattttatga attgacacgt aaaaacgaag
     9121 atatgtattt atttagtcca tatagtgtcg aaaaagaata tggtgtccca ttctcttatg
     9181 taaatattac agctgagtat gatcatctag ttgcaaatcc aaacattcgc aaacaaaaaa
     9241 tcaaagcgcg tgatcttgaa aatgaaatct caaaattaca acaagaatct ggctatcctt
     9301 acgtaatcaa tattgataca gcgaaccgcg agaatccaat cgatggcaag atcatcatga
     9361 gtaatttatg ttcagagatt ttacaagttc aaacgccttc tgtgattaac ggcaaacagg
     9421 aatatgaagt attaggaaca gatatctcat gtaacttagg ctcgacaaat atcgtgaatt
     9481 tgatgcaaag ccctaacttt ggtcaatctg tgcgtacgat gactcgtgca ttgacattta
     9541 ttacagatgc atctgatatc gatgtcgtac caccgatcca aaacggaaat aagctgaatc
     9601 atacgattgg attaggtgcc atgggcttac acacttatct tgcaaaagaa caaatggaat
     9661 atggctcaga ggattcatta gactttacaa atatttactt catgctgttg aattattgga
     9721 cacttgttga aagcaacaat atcgctcgtg aaagaaaaca agttttccat aattttgaaa
     9781 aatcagctta tgctgacggc acttatttcg aaaaatacgt aactggagaa ttccagccaa
     9841 aatcagaaaa agtcaaagca ttgtttgaag gcatttttat cccaactgct gaagattgga
     9901 atgctttgaa acaagcagta atgaaagatg gactgtatca tcaaaatcgc ttggcagtag
     9961 caccaaatgg ctcaatttca tatatcaacg atacgagtgc aagtattcat cctatcactc
    10021 gcatgatcga agaacgccaa gagaagaaaa tcggtaaaat ttattaccca gcgccttatc
    10081 tatccaatga gacgattccg tattacactt ctgcttatga tatggacatg cgtaaagtaa
    10141 tcgatgttta tgcaacggca caacaacacg tcgatcaagg catgagtttg actttattca
    10201 tgcgttctga aatcccagaa ggcatttatg aatggaagac aacaacgaag caaacgacac
    10261 gtgatttgaa tattttaaga cattacgctt tccacaaagg aatcaaatcc atttattacg
    10321 tacgtacctt tactgatgat gcagaagaaa tcggaagcaa tcaatgtgaa agctgcgtga
    10381 tctaatatca tagatagatt cgcttattca tattttaatt aaaggagcat tctcatgtca
    10441 gaaacatatt atgaagcaat aaactggaat gaaattgaag atatcattga taaatccact
    10501 tgggagaagc tgacagagca attttggcta gatacacgta tcccactttc aaatgaccta
    10561 gatgattggc gtacattgtc tcaattggaa aaagatacag taggacatgt atttggcggt
    10621 ttgacactgc tagatactgt ccaatctgaa agtggcatgg atcagttgcg taaagatgtc
    10681 cgcactcccc atgaagaagc cgttttgaac aatattcaat tcatggaatc cgttcatgcc
    10741 aaaagttatt cttctatttt tagtacactg aatacgaaaa aagaaatcga agaaattttt
    10801 gactggacaa atacgaatcc ttatctgcaa aaaaaagctg agcgtatcaa tgaaatctac
    10861 aaacacggta caccgctaga aaagaaaatt gccagcgtct ttttagaaac attcttgttc
    10921 tattcaggtt tttatactcc tctttattat ttaggaaaca acaaattggc taacgtagca
    10981 gagatcatca aactaatcat ccgagatgaa tctgtacatg gaacctacat cggttacaaa
    11041 ttccaattag gattcaacga gttacctgaa gcagaacaag aaacgttaaa agactggatg
    11101 tacaatcttc tttatgagct ttacgaaaac gaagaacgtt atacagaaga attatacgat
    11161 ccaatcggct ggacagaaga agtcaagaca ttcctacgct ataatgccaa caaagcattg
    11221 atgaacctag ggatggatcc actctttcca gatactgcca atgatgtcaa cccaatcgta
    11281 atgaacggga tttcaacagg cacaagcaac catgacttct tctcacaagt cggaaacggc
    11341 tatttgttag gtcatgtaga agcgatgaaa gacgacgatt atttgattgg attggaataa
    11401 ggatttattt taattcaaaa atttttcgaa aaataaaaag agtttagcgt acagatattt
    11461 taaatgtaca ctgaactctt ttttatgctt attcacttgt ttcttccagc acttttggac
    11521 tgtctaactt ttccgaaaca tgttccattc ttctcaataa atagattttc cggatattta
    11581 atattatcgt cttaatcact gcatagacag gtatgcaaat cagcatgcct aatattcctg
    11641 cgacacttcc tgctccaatc aataccaaga tgatcgtcaa aggatggata ttcattgatt
    11701 tcccaaataa caacggcttg atcaaatttc catctaattg ttggatcact aaaatcgaca
    11761 acgccatata caaagcttga aatggagaga taaacaaacc gacaatgatt gctggtaccg
    11821 ctccaataaa agggccgaca tacgggataa tattcgtgat tccgcaaatg accgcaagca
    11881 acaagctata aggctgatcg aatattctca ttgccaaaaa gctcattaca ccaataatca
    11941 aagcatctaa gatcgtactg ctgatatagg cagataatgt ttcgtttaat tcttttaccg
    12001 tttggcgtaa ctctttacga atcgctttcg gaaagaagtg agacaacgca tcaataaatt
    12061 tgtgcccatc cttgaacata aatagcaaaa taaatggaac ggtaaataac aaaataaaaa
    12121 attgcatcat aaatgaaatg atttttgaga cacttgcaga taaactgact aatatgatat
    12181 tgaacaaatt actaagagat aaatttgctg atgctaattg ttcttccaaa ttgaaatttt
    12241 taaactcttc ccgctgcgcc aattcattta accatttgct tgtttcatct gcatacattg
    12301 gcaagctctg ggtcaattga attgtctgtt ccattaactg aggcactaca ttcatgacag
    12361 ttaaaacaac taaaatgatt acgaccaaaa acgtcaataa aaaaccgaac actctcggga
    12421 tccgatgctt ttcgagaaaa acaacgattg gatcgaacat ataatagata aaaccagcaa
    12481 caagcaatgg taaaaaaatt gccgaaagca ttttcaatat tggactaaaa atattcggca
    12541 tttgtaaaat aaagtacacg ccgatgatcg tgagtactag ctccactgtc cagaaaaata
    12601 attttgattg tttaaaacgt gattgcatac atctcctcca aggtcctttg atacttctat
    12661 tctatcattt aacaaagagt tacgcgctga gattttcatt tgcttaaaaa aaacaaaaaa
    12721 aataaacata ttttaatgag tagacgagta cttaaacaaa aacacggttt ggagtagtga
    12781 caaaaattat actgcagtcc ctttttccaa ataaacggtg ttcaaaagct tgcatcttcg
    12841 taataagccc agccaaacga aaaatataag gaactatttt tatttggctg ggcttattac
    12901 ttgatgtagg acatctattt tacaacctca gatcatttat tgcgcaatac tgactaaatg
    12961 caactttgtg atatttttgt gtacggacaa ctcctcgatc acatcggcat aagtgagccc
    13021 ttttggaaga tcaatcgtat agatattttt gtacactcgg tagtcatcaa cgaattttac
    13081 gtcaaaattc acatcttcga ttacgatatt tttctcttca aaataatcgg taagaaaatc
    13141 tttggtttct tttctatgga taaattggat ttctaacttt ttagtcgtag gtacatggat
    13201 cacgcgtttc actactgtga gtgcaatagc aatcccgata aaactgatga tcgcaatgga
    13261 atagtaaccc atgccgatgg cgatccctaa tccagcaatt gcccataaag aagcggcagt
    13321 cgtaagtccc gtcaccgatt gtttcgtgac gatgatcgtt cccgcgccta agaaaccgac
    13381 accactcacc acttgagcaa tcagccttgc ttcatctgaa cgaatcgttc cagcaagctg
    13441 tggatactct aaagcatctc ttaatgcacc actagcgatc tcaacttgga tcaatgcaat
    13501 gatacatgct cccatacaaa ctaaaatatg cgtacgcata cctgctggac gatttttata
    13561 ttgtcgttca aaaccgattg ctccgccaaa tagcatagcg atccctaaac gtaaaataat
    13621 ctctggaatt gtcaatacca tatcattcat ctaaacaccc tccagtccaa tatatcatgt
    13681 ctgaaaggag cagacaaaaa aagtgcaatt tttacctctt acgaaaaaag gactgtgaca
    13741 aaatttttgt catagtcctt tttatgaata aacggtgttc aatcagctga ccctcggtat
    13801 tactcaacaa acggcaaaat atggggagct atttttcctt tgtcttctct aatactcagg
    13861 tcaaaacgct gatttcacaa cctctttagc ctactattga aaattacaac ttacttgctg
    13921 cttcttcatc gatgatcaca acaacattgt cgtgattttg caatacactt gctggcaaat
    13981 gattcgttac aggtccttca atcatccctt tgattgcatc agctttgctt tcgccaaatg
    14041 ccatcaagac gatttcttta cttttcataa ttgaaccgat tcccattgaa acagcttttg
    14101 ttggtacatc ttctgcttta tcgaagaatc gtttgtttgc attgattgtt gattctgtta
    14161 agtcaactac tgaagtcgtt ccgtcaaatg aagcaccagg ttcattgaaa ccgatatgtc
    14221 cattttgccc gattcctaag atttggatat cgattggatg tgcatcgatg atttgatcat
    14281 actttttgca tgctgcttcc agatcttctg cttttccatc tggtacaaac gtttctttga
    14341 atggtttttt atcaaataat tggacatcca tgaaatgacg gtagctttgt tcatctgaac
    14401 cgcctagtcc aacgtactca tctaagttta cagaagtcat ttcactgaaa tccaaatcgc
    14461 ttgaagtcat ttctttatat aaagtaactg gtgtgcttcc tgttgccaat cctaatactt
    14521 ttgcaccttt gttcatacct tctttgatga tttcaaatgc tttttttccg ccttcttcag
    14581 cgttttttac acgaatgatt tccatagtga ttgctccttt atattttggt atagcccaat
    14641 tatagcattc tttttttctt tttacaataa ttagtataga ccaaattgca tttttttcat
    14701 tttcttgaca ataaatagga atccgtgtta ggtttcataa gatggttcaa tcaaacaatc
    14761 catgttgcat gaaaaccaag caagtgattc actgtggacg ctttatgtct gcagtttttt
    14821 tatgaaaaag gagttattat gagtatactg acattaaaca acattaccca gcaatttggc
    14881 gacaaaatcc tctatgaagg tgtcgatctg caattaaatg ctggcgaaca tcttgggctg
    14941 attggacaaa acggagccgg taaaagtaca ttgattaaaa tcatcactgg tgagatctta
    15001 ccagatgacg gacagatcca ttggcaaaaa aatattcaca aaggttacct cgatcaatat
    15061 gtggaagtcg atgaaacatt aacaatcgaa gaatttctaa aaactgcgta cgcaaaagaa
    15121 tacgaaaagg aagaaaaaat tgcttttctt taccaaacat atagcgagac cttcgatgat
    15181 tccttgctgg aaaaagctgg gaaactccaa acggaattag accaaggagt gttttatcaa
    15241 atcgacacct tagttgctga gatgagcagc gggttgggga tcgatgtctt aggtttagat
    15301 tcactgttga aaaatctaag tggtggacaa cgttcaaagg tgattttagc aaaactactt
    15361 ttagaaaacc cagatgttct gatcttagat gaaccaacca atcacttaga tgatcagcat
    15421 atccaatggc tgacgcagtt tcttcaggat tttgacggtg cgtatatcgt gatttcccat
    15481 gaccaagaat ttcttgatca gatcaccacc catatcgcag acatcgagtt tggaaagatt
    15541 actaagtata ctggccatct aaaacaagca ttgaaacaaa aggaacaaaa tcgcgaaagc
    15601 tatttgcgcc aatatcatgc tcaacaaaaa caaatcgaaa agaccgaagc ttatattcga
    15661 aaatacaaag ccggttcccg atcaacgatg gcaaaaagcc gacaaaaaca attagataaa
    15721 atcgaacggc tgacgcctcc agcttcagca agtaaagctg tctttgattt tccattctcg
    15781 cctattgtca cgacattagc agtggaaaca actgatttag tcatcggata tgatcgaccg
    15841 ctacttgcac ccatcaattt gaccatccgc tttggtgaaa atattgccat tcgaggcttc
    15901 aacgggattg gaaaatctac tttattaaaa acactgatcg gtgagattga aaaaataagt
    15961 ggcgatttcc acttcccaga taacacaaaa atcaattact tttcacaaga tctgtactgg
    16021 gaaaatccat tagaaacacc actacaatat ttaagcagtc aatttccaaa agcgactatc
    16081 aaagaattac gtcgccaact agctaaagca gggttagtta atcaattagc aagtgaacct
    16141 ctcactacat taagtggtgg ggaacaaact aaggtgaaac ttgcacagtt gacaatgaac
    16201 caaggtaatt tactgattct cgatgaaccg acgaaccata tcgatcaaga aacgaaagaa
    16261 agcttacaag acgggataca gaaatttcca ggaacagtaa tcatcgtctc tcatgaacaa
    16321 gaattttatc aagatcttgt gcaacgagtc attgagattg aaccaaaata aacgtttgat
    16381 tgaatagttg aaaaaaagga gctgtgacaa aagttttgtc acaactcctt cttctgaata
    16441 aatggtggcc aattagctga ccttcggtat tagctcaaaa aacggcaaaa tatgaggagc
    16501 tatttttatt tggctattct tattacttga tgcagaacag cttttcacaa cctcttttcg
    16561 tctatttttt attttcgaga tttctctttc ctttaaaaac aactgtttga aagaagaacg
    16621 cacatccttg aaaaacaagc ccgctaaccg ttacacctaa ccagccatac tgctgccaca
    16681 ttgtcgtccc tagcaatgaa ccagccgcac ctccaataaa gtacataaac ataaatacag
    16741 acccgtggtg ctagttaatt ttaatatcct aaagaatatc tcaaagttcc ttcaaaacgt
    16801 cgttgtgctc gacttaatag aacactgtaa gccagtaata tgctttcaaa tctactctct
    16861 agccctttaa cagaacgtga gttgaaattt tgacaaccta atttttctaa ggaagaaaaa
    16921 acagtctcaa ctgtttttct ttgttttcca agtaagctgt ggtctagttt ttctgattta
    16981 tccatgtttt tacgtggc
//
LOCUS       DAZBTF010000059        16998 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000059 DAZBTF010000000
VERSION     DAZBTF010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.059"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(509..3214)
                     /locus_tag="ACHGNS_002051"
     CDS             complement(509..3214)
                     /locus_tag="ACHGNS_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFN6937978.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(3520..4467)
                     /locus_tag="ACHGNS_002052"
     CDS             complement(3520..4467)
                     /locus_tag="ACHGNS_002052"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFN6937979.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            4657..4926
                     /gene="rpsN"
                     /locus_tag="ACHGNS_002053"
     CDS             4657..4926
                     /gene="rpsN"
                     /locus_tag="ACHGNS_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFN6937980.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(5102..5464)
                     /locus_tag="ACHGNS_002054"
     CDS             complement(5102..5464)
                     /locus_tag="ACHGNS_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFN6937981.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(5475..6596)
                     /gene="alr"
                     /locus_tag="ACHGNS_002055"
     CDS             complement(5475..6596)
                     /gene="alr"
                     /locus_tag="ACHGNS_002055"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFN6937982.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(6612..6962)
                     /gene="acpS"
                     /locus_tag="ACHGNS_002056"
     CDS             complement(6612..6962)
                     /gene="acpS"
                     /locus_tag="ACHGNS_002056"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFN6937983.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(7092..8603)
                     /locus_tag="ACHGNS_002057"
     CDS             complement(7092..8603)
                     /locus_tag="ACHGNS_002057"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFN6937984.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(8925..10277)
                     /gene="murF"
                     /locus_tag="ACHGNS_002058"
     CDS             complement(8925..10277)
                     /gene="murF"
                     /locus_tag="ACHGNS_002058"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFN6937985.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(10430..11506)
                     /locus_tag="ACHGNS_002059"
     CDS             complement(10430..11506)
                     /locus_tag="ACHGNS_002059"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFN6937986.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            11702..13447
                     /locus_tag="ACHGNS_002060"
     CDS             11702..13447
                     /locus_tag="ACHGNS_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFN6937987.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(13727..14164)
                     /locus_tag="ACHGNS_002061"
     CDS             complement(13727..14164)
                     /locus_tag="ACHGNS_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFN6937988.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(14148..15521)
                     /locus_tag="ACHGNS_002062"
     CDS             complement(14148..15521)
                     /locus_tag="ACHGNS_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937989.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(15843..16331)
                     /locus_tag="ACHGNS_002063"
     CDS             complement(15843..16331)
                     /locus_tag="ACHGNS_002063"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6937990.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(16353..16955)
                     /locus_tag="ACHGNS_002064"
     CDS             complement(16353..16955)
                     /locus_tag="ACHGNS_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFN6937991.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         4797 a         3627 c         3081 g         5493 t
ORIGIN      
        1 taaaagtcaa taaactttaa gaaaataatt ggaaaaaaac agaaaaatat caagcgcttt
       61 ttcaaacgct ttaatacagc aaaaatctaa cattttttta tgggcttttt ctttgaattc
      121 tataataaaa ggatagaact tgacttagaa aatcaagttc tatccttttt gatatcttaa
      181 cttttggaag agagatgtaa ggctgctttc tacccatcat tcttactact cgaagcatag
      241 cgcagttgtc acaacctctt gataaacggt gtttaaaagc ttgcatctgc ggtaataagc
      301 ccaaccaaat taaaaacata agaagctgtt tttatttggt cgttcttatt acttgatgca
      361 gagcagcttt tttacaacct cttggtacac ggtgttcaat cagctgaccc tcggtattag
      421 ctcaaaaaaa cggcaaaata tgaggaacta tttttccatt tttttctcta atactcaggt
      481 caaaacactg atttcacaac ctcttttttt aatgaaaaat atggctctca tgcgccgctt
      541 ctttgaattt cgtcattttt tcttcgatga acggatagat tgccgttcct acgattccaa
      601 agacaacaaa caaaccgatc atgatccaga ttgctttcgt agcatttggc caataaattc
      661 ctcctgcaga ttcccgtaac aagtttaccg catgagtaaa tggcaagaac ggattgatca
      721 tttggaagaa tttccctgag acttggatcg gatagttccc gcctccgcca gagatcgaca
      781 gtactaagat gataatagcg atccctttac cgacattatc gaataatgca gccaagacat
      841 agacgatcat catgaacgct aaggcgatca agacagcaaa tgacacgctg tagaccggat
      901 tttggacatc tactcctagt aagaaaatat tccctaatgt aacgatcaag ctttgcgcaa
      961 tcgccattgt caaaaatgtc agcatacgag caacgaattg ttctcgtttc gtaaaattgt
     1021 ttcggtcttt ctttcctaaa tagtattctg tcgtagcaac acttgaaaga agaacagcgc
     1081 cgacccacag acatagtgca gtataaaacg gggtgctagc tgaaccgtta tttgcgattg
     1141 gataaagcgc attggtttgt agctcaacag gttgtgtaaa gaagtcactt tcttttgttg
     1201 cgtctagttt caataatttg atgacttcgc ctagatcgac ttcttgttcg ccttttcgga
     1261 tcgcgtctgc tgctttttgg ataccagctt tgatatttgg ccagtcattg ttgatcaagt
     1321 ccgcagcagc gttcacagct gtttcgactt ctggcatttt ttcatttact gttgtcaatg
     1381 taccggtgat ttcatttttt agtgctggcc aatcattttg gataaaatca gcggccaatc
     1441 ctaatttttg ctgaatgatc ggtaattcat tgttgtatag gtctgcccct tcattgatgc
     1501 cattgacgat ggtatccata tttccattca gcattgtatt ggcatcatgg atttcttgtc
     1561 caattgcagg catttctttt tgatattttt ctaaaattgc cactgcattc gttacagttg
     1621 ctttcgttga gctgagaagc gaatcaaagt cgatctggtc cgctttgctc agtgcgcctt
     1681 gtgcagtccc aatcgtatcg atgacttggt ctagtagaga agtgacagtg gaactgattt
     1741 gacctgtatt gataccagca atgatgccat tgatattatt aacaacggca tcaatttgat
     1801 ctagcaattc atcaatagcc ttcgtgtctt cattttgaac agctgtgttc aaagcagata
     1861 ggcggttttg cagattgctt agcaagggtt ttaattgatt tagctgatca ataattggct
     1921 gtaatttcgt atttccagca cttgtttgca atttagtcag gaactcgacc agtttatcga
     1981 ttgcattttg ttgttgagat attccgtcaa taaagccgtt caatacattg ctgatgtttt
     2041 tcttttcatc atcagtcagc ttaccatctt caatagctgt tcttacagta tctattaacg
     2101 aacctgtcgt tgttgccact tgtccaattg acttcaatgt aacatctaca ctgtttgtga
     2161 tgcttggtat cgcatctttc agctgagagg cacctgattt tgtttggttt gctagatcac
     2221 ttgcctgatt ttctaaattt ttcacatctg gcaagatatc ttgtacttgt tggatgatcg
     2281 tcaagccttg ttttgcttca tcgattcctt ggttcattgt ctcttggatc gaagcaaaat
     2341 cgccgtcgat ctctgctagt tgcttaccgg cattttggat ctctgggatt ttttcttgca
     2401 aatcaaggat gatttttgct tgatctttca actcaggcat tttgtcattc aatgcaacga
     2461 ctttttcacc catctcatct actttaggga tgtaatccac gaattcattt gctttattca
     2521 gtttttcctt gatttcaggt agttgacttt gtaattcgag gacttgtttt gtgtagccgt
     2581 cgatcgtgtc caaattttca tctgttgata aaatcatatc tttaacttta ttgatactta
     2641 ctaggttcgt atcgatgtcg taacctatct cattgaatac tttcaataaa gtcgaactgg
     2701 ctgttttgat aaattcattt gtgatttgcg attggatcga acttgctcct ttatcggtga
     2761 ttttcggtgc aatcgcattg attttttcat ttaccgtata ttcgattttt ggtttcttga
     2821 tatcaccact agtaaaactc agcagatctt ctgagaaatc ctttggcaag taaatacctg
     2881 cgtaatactt tccggaacgg actccgtctt ctaattgttc ttttgaatca acgaattgcc
     2941 atcctaattg tttattttta tgaagcgatt cgatgacttg ctccccaatg gccacttctt
     3001 taccttgaaa ctctgctggc ttgtctgcac tgtaaacagc aattggcagc tctccagtat
     3061 ttccatatgg atcccataat gctttgatgt taaaccacgc atatagcgaa gggatgatca
     3121 tgatcgcgat gatcagaagt gttgcaatcg ggtttttgaa aatccgtttc cagtcaagca
     3181 aatataatcg aagcgtattt ttgattgact gcatagttct cccccttctt cttttttcag
     3241 ctgaattact ttaccatgta tttcatactt ttttcaatcg gcttttttca aaaaagtcga
     3301 atttttggac atatcacggc aattgtctca ttgtaatctc ttacatattg agaaataatt
     3361 aaggattaca gcctttactt tatcatgaaa taaaagggaa agataatttt atgccaaagt
     3421 ttttatctat tatacgtata cgcaaaaaag ctaaaacatt ttttgtcttt gcccctacaa
     3481 aaatgtttca gcttttttcg ttttttattc ctgtatttct tatttttcga atgacaggat
     3541 gattttccca gcaggaggat gtccttcaac tagctgatgt gcttggatga catgcttaag
     3601 tgaagcagac agattcattg aaataaaaac atgaaaggct cctttttggt aggctttgat
     3661 tatgccagca aaggcttctg catctagata ttcctttctt ggaacaaacg attcataaaa
     3721 gccttccttt ttttgccgca aatctagatc tgggaggtca tttaatgcaa catacctgcc
     3781 gcctggtttc atgatctgga tgccggtttc tcctttgata cttcctttcg tcgcatcgat
     3841 tacaaggtct gcctgatcag caaattgcag acctggattt gttttatcat aagcagcaaa
     3901 tgctgaagct cctaattttt ttaccttttc ctcattttta ctggatgcac tggttaagat
     3961 tcggattccc ttttcatgga gaagctggat cagacttgac ccgaccgcac ctgatgctcc
     4021 tagtatcatt acagtatctg tcggctggat ttccgtcaaa tggttgatta gattataagc
     4081 cgtaattcct ggcgtgacca ttccggctgc ttcttcccaa ctcattttat ctggaatttt
     4141 tgctactttt gctgatggca acacgacttc ttctccgtaa gtcccgccga ctgcatggac
     4201 agctactcga tcacctacat gaacagttgt gacatctgaa ccaatctctg tcacaatacc
     4261 agcgccatca ttacctggta cataagggaa tttcaatgtt cgtatctctt tcatttctcc
     4321 taaacgcaag gcaacatcat aaggattcac actgaatgct tttatttcta cacgaacatg
     4381 tccagaaccg acttctctgg gatgagcatc gatttcttca aaaacatctt gtgcttctcc
     4441 gtaaccgtta attgcaaatc ttcgcatggt caccatctcc ctttttcttt aagtttacgt
     4501 ttatgccttt attttgacaa aaaaaatgtc ttttcgtttt ggcgcatgat tcaaaacaaa
     4561 atagttgtct tttttcgcgt aacgtgttaa attatttatc taaaagcgta aagattacga
     4621 tttacgctaa taaaaaataa ttggaggaat ttgttgatgg ctaaaaaatc aaagattgcc
     4681 aaagcaaaaa aacagcaaga aacgattgaa cgctatgctg ctttacgcca ggaattaaaa
     4741 gcgcaaaaag attacgaagg cttgcgtaaa cttcctttag atgctcgtcc agaacgattg
     4801 aaaaatcgtg atttaatcga tggacgccct cgcggataca tgcgtaaatt cgggatgtcc
     4861 cgtataaaat tccgtgaatt agcacataaa ggtttgattc caggagtaaa aaaagccagc
     4921 tggtaatcca agtctggctg taatgtaaag gaggagggcg catggaccac tccttggatc
     4981 atcaaaaaag atacgttcgc ttcttctagc agaaatgctg gtactagaag cagacgtatc
     5041 tttttttacg cacagctggt ccttttttat atgtaaaggc tgcatggtta cttcgctata
     5101 tttatttagt aacaggagtc agtgcttcga tacccacact aattttcaat gctcggtcaa
     5161 cttctgccat aatgggtcga tccaaatggc agaccttttc tttcaatctt gatttatcga
     5221 tcgttctgat ctgctccaac aggataacgg agtctctttc tattcctgtt ccagtagctt
     5281 tgatcccaat gtgagtcgga agcttaggtt tagccatttt tgccgttatg gccgcaacaa
     5341 tgatcgtcgg gctaaaatgg ttacctagat tattttgaat aaccaaaact gggcgtgttc
     5401 cgccttgctc cgatcccacg actggagaaa gatcagcaaa ataaatatct cctcttttta
     5461 ccataggatc ctccttattc ttgatattct cttggtatcc gctgtcctaa tccgcaagcc
     5521 acttcatagt ggatcgtacc taattgatct gctatatcct gcatagtgat ttctttgtct
     5581 gcatttttgc cgataagcgt cacttttgtt ccgacaggaa attcttgagg caatcggatc
     5641 atcagttgat ccatacatac acgcccaatc gtttcacagt agttcccttc taccaataga
     5701 gaaaaaccct gcatctttcg tggccagcca tctgcataac cgatcggtat cgttccgatc
     5761 cactctgctt caggagtgat atatgtttct ccatagccga ttccttcacc tgctgggagt
     5821 tttttgactt gaatcaattc agaaaccagc tcaagtgctg gctgcaatgg ataaacttcc
     5881 ggtagtgcgt gcccagaggg attaagtccg tacattgcta ctccataacg aatcatattg
     5941 ccgattgttt catcatgcca taaagccgtt gcactattgc tgacatgtac atatcgagga
     6001 agttcgggaa gttttttcag cacttctata aagcgttctt tttgcagatt ccaatatgta
     6061 tcatctgcct gatctgctgt agaaaaatgg gtaaataaac cttcccattc taatacacga
     6121 ctttctttga tgaatgcagc tgcctctttt acttcttcag gtgtacaaaa tccgatacga
     6181 cccatcccag tgtctgcttt tagatgaatc gaaagaggcg tctctagctt acttttctct
     6241 aaaacagcag ctgctgcttg caaccattcc cgtgtacctg cagttactga taaatcatga
     6301 gcaacagcta cagtcagtgc gtcgtaagga accatattca agataaggat gggctgagta
     6361 aatccagctt ctctcaactc gactccttca tctaaatttg aaacacaaaa gcccgaagct
     6421 ccacctgcaa ctgctgcctc agctgtttca atagctccat gaccgtatcc gttcgctttt
     6481 actactgcaa acaattcttt tccttgcgga agtcttgcaa cttcatttgc tacattttca
     6541 gttatagctt ttttatgtat cactgctttc gttgggcggt gccaagcgac aaccatcgtt
     6601 tttccttctt tctagtcttc caaaataatt tgtgcaaaag ctgtttcatt tgtatgagta
     6661 atactgaccc atacattgcc ttcatgaggt gaacgcgtca caacaggctg accattttca
     6721 ttttttaaaa tctcgacatc ttgaaaggta acttttccga tcccagttcc ccaagcttta
     6781 gagaaagcct ctttgcaagc gtaacgcccg cctaaaaatt ctacttggcg atgaaaaggc
     6841 aaggactgga atagtttcat ttcatcagag gtcaagattc gtgcaatgaa ttttggtttt
     6901 tcttcgatca gccgagtgat cctaggaagt tcgactgcat cgatcccaat tccttttatc
     6961 atatgctttt ctcttttcaa taaatttaga acactctcat tctaccaaat tataaccatt
     7021 ttgccacaaa taagtccaca aataacaaaa atgaaagttt ctcttaaaaa agagaaactt
     7081 tcattttatg tttaatcgtt atttgaacgg attacaaatc cgcgtttttt atcattgtga
     7141 cgtttaccgc catctttttg gctgttatta tttttgctat aaccgccttt tgatttgttt
     7201 ccacgataat taccgccgtc acgacgatta cggttgtttc cgccaccacg tttgccattt
     7261 ttattgtatc cttttttctg cattggcaat gggcgttctg gtgtgatctt cactggcact
     7321 gcatcagctg gatcttttgc tgtcgttttt agcaaaagtg caactaaatc ttgtgcagaa
     7381 tattcttcta ataatttatc tgctgtttgc aggtatttat ctagaccatt ttctgcaagt
     7441 tttgtttcga tttgttcaac tgcagcacct aattgacctt tgaatgcttc tttttcggtt
     7501 ggtggacgta aagtcgtcat ccgttttttc gtcaagtttt caatcacatg caagtagtcc
     7561 atttcatttg gtgtaacgaa agtcacagac ataccgcctt ttccggcacg gcctgtacgt
     7621 ccgatacggt gaacgtagct ttctggatct tgagggatat catagttgta cacatgagtg
     7681 acacctgaaa tatctaatcc acgtgcagca acgtctgtcg ctacaaggat atctaaatga
     7741 ccgctcttaa atgaacgcaa tacgctcatt cgtttttgtt gagacaaatc gccatggatt
     7801 ccttctgcac ggtaaccgcg tgcttctaat ccgcgtgcca attcgtctac acggcgtttt
     7861 gtacgtccga agacgattgt taattcaggt gtttgaacat caaacaagcg agtcatgata
     7921 tcgaattttt cgtattcttt tgcgcggaca tagtattgat cgatcaagtc agctgtcatt
     7981 tctttcgctt tgatcttcac atgatgtgga tttttcatga atttaacgcc aatattcttg
     8041 atagctggcg gcatcgttgc tgagaaaagt aatgtttgtc tttgatcagg aacttgtgaa
     8101 atgatttttt cgatatcttc caagaagccc atattcaaca tttcatctgc ttcatccaaa
     8161 actaatgttt gaacggttcc aagtttcaat gtgtggcggt taatgtggtc aagcatacgt
     8221 cctggtgtac ccacaacgat atgtggacga tctttcaagc cacggatctg acggccaata
     8281 tctgctccgc cgtaaacggc ttgtacacgt acttttttgt cgcgccctaa acgatataat
     8341 tcttcttgtg tttggatagc taattcacgt gttggtgcga taactaaacc ttgtaattcg
     8401 tgacgatctg gatcgatttt ttctaacatt ggtaatccaa atgcagctgt tttccctgta
     8461 cctgtctgtg cttgtccaat aacgtctttt ccagctaatg ctagtggaat cgtttctgct
     8521 tggataggcg ttgcttcttc aaaaccagcg cgttcgactg atttcaacaa ttctggtgat
     8581 aattctagtt ctttaaattt caaatgaatc ctcctatatg gttgttaact gaggatgtag
     8641 cttttttgct accccgtcga tttgttcggt caaagtgtat tgtttctttt tgacagtggc
     8701 cacgcacgtt tttatccatt cttagcccct gtggcaccac cgaaaaatca ctttgcgaag
     8761 tttaggcatt agaaaagcca aacgggtcgc gttcagcttt atcattatta tgacatctgg
     8821 cttcaacaag ttgtttctat ggcaaaacca gtccaatttc tcttcatctt tcggcaatag
     8881 gttattctaa catagttttt cgttttctgc aagtttacag atttttattt catttgctga
     8941 agttgttcta ccacttcaaa taagcccatg ctgttgctgc cttttagcat aatactatct
     9001 gttgcttcaa gattttgttt gatagcatca atcaatttat tcttttcttt ttcaaaatag
     9061 aacacaggta gttgggggta tttttggttc aattcttctt ttagtgcatg catttgttcc
     9121 ccataaagga aaatcatggc gaatttctca ggatcgatat gttcactcat ttgcgcatgc
     9181 atatttaaag actctttccc taattccagc atatctgcta acacagcgat tctgcgtcct
     9241 tttgttggca gatttttaaa agtatctaat accagtccca tggctgttgg atttgcattg
     9301 tagacatcgc tcaaaatagc tgcaccattt cctgccgtta gccattcggt tctgttttga
     9361 gtgatagaaa catgggcgag cccttggcgt atctcaggta tggacagtcc aaagaagcgc
     9421 ccgatcgtat acgcaatcaa agcattcgtt acattataac ttccaggcag cgggatcgta
     9481 aatgtttctc cttctactac aaaatcggtt tgtgcttttc cttttgctgt cacttccgca
     9541 tgaacatcgc catttccaat accgaatgtt gtgactgttt gtactaatgt tgccgttaaa
     9601 ggtgtcagca acggttcatc tgcgggcaca acgagtaatc cgtctgatac aagtccgtca
     9661 gtgatttcca ttttggcttt agcgattcct tctcgcgacc caagattttc tatgtgtgct
     9721 tcaccgatca ttgtgattgc tgcagctttt ggttgagcca taagtgaaag ttccgtgatt
     9781 tcattggcgt gatccatgcc catttccaaa acgatctttt ctgtatcttg gggcatgtgc
     9841 aaaatcgtgt aaggaagacc aatattattg ttgtaatttc cttgcgtctt atatgttttg
     9901 tatttttcag acaagacaga cgcggttaga tctttcgtgg tggttttacc attgcttcct
     9961 gtaattgcaa cgacttctgc gcctactttt tccaagtaat aggttgccaa ttgctgcatg
    10021 gctttcaaag gatctgctac aggaataact ggaaaatttt ttggagcttg ttcaacaggc
    10081 aagctccaaa atgaagcaga tgctccgttt gtgatcgctt gctggataaa ttcatgtcca
    10141 tctcgcgtac cagcaagagg aacgaataaa cttccttcgg ttatcttccg gctgtcaaat
    10201 tcgacattgg taatctttgt cagtgcagag cattcgctgc ccacggcttg tgcaatttct
    10261 tgtattgtta gattcattga gatctctcct aaaaaaggtt gtgaaaaaag cgtttggcct
    10321 aaacggcggg ttcgcatgac tctcacttcc aagtaaattt ctctattcgt atttagaagc
    10381 tagaatgaac gcggttacgc aatcactcca gcctcttctt tatttctctt tattcatttt
    10441 tttcaaaaaa agattgacgc tgatggtatc gattcattcc taactggatc agttcttcaa
    10501 tcaaatcacc gtatttcaag cccatatttt cccataagag tgggtacata ctgaactccg
    10561 taaatcctgg catagagttt aattcattca ggaataattc atttttattt gtcaaaaaga
    10621 aatcgcaccg gctcaatccg cttccaccta acatcgtgta agctaacttc gcgtactctt
    10681 gcgctttttg ataaacttct tctggcactt cggctggaat ctgcatttcg attttattat
    10741 tgatatattt tgcttcataa tcatagaatg ctacgtcttt tacgacttca ccaggcaaag
    10801 tcgtccgaac gtcttcattt cctaatacag caacttcgat ttcgcgcgct tcaattcctt
    10861 gttcaacgat tgctcgagaa tcatactgat aggctgttgc taaagcattt tgcagctctt
    10921 ctcggttttc tgcttttgta atgccgacac tagaacccat attcgccggt ttgacaaaca
    10981 tcggataaag caaagaacct tcacattgat caaatacttt tttaggattt tctttccatt
    11041 gattcttaag tactggtaca taaggaactt gcggcacacc agcagcttgt aaaatatact
    11101 tggtcatgat tttatccatg gcacatgcac tggtcaatac gcctgcgccg acataaggca
    11161 tattcaatgt ctctaagaag ccttggatcg ttccatcttc cccgtttggc ccatgtaaaa
    11221 ctggaaaaac gatggctcct tcttctttga tttcgcccgg attgatcact tttcctgtaa
    11281 agccttcctc tgtctgtcca cttgggtccc atgaaagatg caagacatct ttgctggcag
    11341 gtttttctgt tagtaatgga cctttgaccc attgtccttc ttttgtaata aatacgagtt
    11401 gaacttggta ataattataa taaatggcat ttaaaactga aaatgcggaa agaatggaca
    11461 cttcatgctc tgcgctgcgt ccgccatata gtaaagtaat cttcaaaaga ttgtcctcct
    11521 gtattctaaa tcgttcagtg atttactctc atattttagc acaaaacaaa aataaacgaa
    11581 taacttattt ataaaaaaac gagccagctt ctaaaagaac gagcgtcatt tattttttgt
    11641 cagctagatt atttgtcccg ttttttataa aaagttatga taaagaaaag gagggatgat
    11701 gatggcgcaa acaaatccac gcagccgctt ggcagaaatc attcgagtgt tcatcaatta
    11761 caatgtagtg ccaaactttg tacagcaaaa aaatcccgaa caggtaaaaa aagcctttga
    11821 agaactaggc cccaccttca tcaagatagg acagatgctt tctgttcgag aagatttgct
    11881 ttcttctgcc ttcactcaaa cattcaaaac attacaagat agtgtaccta gcgacacatt
    11941 ttcaacggtc aaaaagacga tcgaaacaga actttctctt tcattatcag atatattcga
    12001 tgatttttca aaatcgcctt ttgcctctgc ttctatgggg caggctcatc gtgccaagct
    12061 gaagaacggt gacgcggtag tcgttaagat ccaacaccct aacatcgccg aagagatccg
    12121 acttgatctt caactatttg aacgagcgat ccctttgatc aaatacattc ctgaaactag
    12181 cgtagttgat ctaaaagggg tactccaaga agtaaaacgt tccttgatca atgaaatgga
    12241 cttcttaaaa gaaagtcaaa atggcgaaca attctatcaa aagaacaatg gctggaaaga
    12301 gatccgcagc cccaagattt atgacgcttt ttgttcgaaa aaagtgatcg tgatggaaga
    12361 aatgtctggt aaaaacttga atcatttaat gaacgccgaa aacaaaactg aaacttttat
    12421 cactggaatt caaaacaagc agctgaaaca agaagttgca aagcttttag ttgagaactt
    12481 tatgaaacag gtgtttgatg atggtttttt ccatgctgat cctcatccag ggaatctgct
    12541 attccatgta ctaacaaaag aagaacagac acaagctagc cgaaaaactg aaacagtcca
    12601 tgaaaaagag ttcggttcct ttgcttttcg agcctccact tctgcagaag accctgttgc
    12661 gccatatacc atcaactaca tcgattttgg tatgatgggg catctttctg caggacttag
    12721 gcaaaaactc acacaagccg ttttagcgct gtatacgaaa gatgcttatc gaatcgaaaa
    12781 agcggtcctt cgactttgtc agcaagaagg ctcgtttgat gaaagtcggt tccaccaaga
    12841 actgacaagt tttcttgaac agtattatga ttctcctatt gatgaaatca acttgcaaga
    12901 agtattcaca catgtcgtga cgatttgtca tcaaaataat ctgcagttcg atcgtgatat
    12961 cacgcttttg ctgaaagcat ttgggacatt agaaggggtg atccgcgtcc tcgatcctga
    13021 agtttccctc atggaagtcg cttcgccttt tgctcaacat tattttctca ctcatctgga
    13081 tgtagaagat acattgaaac aaagcggatt agacttattg gaagggatga aggctgcgcc
    13141 taagatccct caacagctcc accatctatt agaaatgtgg acttcgggac aaggaaaagt
    13201 caatttggaa ttgaaaaagc aagacaaatt attgagcagg atcgaaagta tgatcaatcg
    13261 gcttgttttc ggtatgattt tagctgcatt gatcgtaggg tcctccctgc ttgtccaagc
    13321 agcacctgta gaaaacgcag aggtcgtttc cttattaggg atatttacgt atgctattgc
    13381 cgcattcgtc attatttttt tggcaatcga tgcattgatc caaatgtata agaagaggaa
    13441 aaagtagaaa aaggttgtga cagaaagaat cagcttcaag aaataagaag gggttttaca
    13501 taaattgttt ctcaaatttt tgtgaaaatt cggcttattt ttcgaagagg ctacttctga
    13561 aacaccgaga ataaaaaatg tgaaaaagct gttctgcacc cagtaataag aacaatcaaa
    13621 aagggaacat ggaacacatt tatcctgttt ccctgttccc tttttgatgt attcgagcat
    13681 aaataaaata ccttcgttac ctactatctt ctgtacttgg taaaccttag tcaattgcct
    13741 tttttaacgc ttgacgaaaa gacatatgat ctttctccgt aaagggtttt ggaccctttg
    13801 tccttatgcc agcttttctt aactttccac tatgataacg ttgttctaga agcgtgccga
    13861 tattcgcttc gtcaaattct tttccagagt gatggaacac tcgcacaccc ttcggcaata
    13921 caagtgaagc taaaccataa tcttgcgtga tcaaaaagtc attcttctta accataccga
    13981 cgattttata atccgcacta tcagctccgc ggtctacgta gacgatttgg acatgattag
    14041 cgtattcttt ggtcgtataa tggtccacac tggtcacgat aaccacgtca agaccatagg
    14101 attctcctaa ttttatgaca tcttctttta ctggagaacc atctccatca atgaatagcc
    14161 tcattctttt tcgccttctt ccataaatga taaacagtta ctaatccgct cagtaaaaga
    14221 ccaacattga tccattttgc caagataagc agttgtacac tttctatcgg cactgattca
    14281 tggttcagcg tgatcaataa tagatggatt ggctctccca tttcttttgc taaaacaaac
    14341 aaaagcgtca tcaataaaaa ctggtggatc acactccctt tattttgagt agtatacttg
    14401 acaacgatcc ggcgatccat tggaatagct gtatcagttt gataatattc cttatttagc
    14461 cagctgcctg ttactacgtg ccagaaacta agcagaaaga cacctatcgc aaaaccattt
    14521 ggaatcaaga ggaccaataa agaagaactc agtccaagca attgcatagc caatggagac
    14581 catgttaaac gtttcaaaat ccatggtccg actatttttg ctgcaatcaa gcctgctgca
    14641 taaggcaaat acataaaaat cgaaagtttt tctaatccgt acagtatgcc aacataaaaa
    14701 ctgctaaata aaaaaaggaa attccctagc attccattta gaaaagtaaa cagatttagc
    14761 cagcttggca acttccattc cttcttctgg tgtcctaaaa caaacacgaa caagatgaac
    14821 agcagcaaga aaccataaat agcaagatca aaaagccttt catcgaataa caatcgggca
    14881 tttctcaaaa gaaacaataa gccaaagaat atcaaaaata aaacaagctc tcgcgtagct
    14941 accagatttc ttttgatgtc tttgaaatcc agttcgtaac gcggataatg actaaccgta
    15001 tggtaggcca tgacgtaaaa aagtgtataa atagtaaagc ttgctgcaat ctgagtcggt
    15061 gcaggcaact caatcgcttg atacaggaga aatagccaga tagctgcaaa taagtacttt
    15121 ttcccatcca tattgataaa accttgcgtc ttttcgtgaa aattgaccgt ggtattagca
    15181 ggtacaagcc atgctgcact cagccctagc aaaacacttg aaaaataaaa taaaggaaaa
    15241 taaaatacgc ctaatattcc aagcaatgaa ccagcaccac caatcagcag agcgatcatc
    15301 agcaacgtgt aactatctag accaaatcgt agactgttga ctaaaaatag tccagtcatc
    15361 cgaaaggcat aaaacagcgt aaatggcaaa atcgtcagcc aattatgcga gtctgttagt
    15421 cccaaaaata aaagataagg aataaaaata accgtgttac ttaagataaa gggcgttgtt
    15481 tgtgtaaaat tacgcccaaa tcgtttgagt ttttcgaaca tgacaatggc tccttaaaca
    15541 gtcttataac taatctgctc ttcgatttta gcattaaagg ggaaataaac caattttaca
    15601 gaatatattc ttgtaaaaaa aaatacagaa agaagcataa gcttctttct gcatttttac
    15661 ggtacttgat tactcaagca gacaagccct tctccacaaa agtcttgccc gacacccctg
    15721 catccggcag gtgataaaga tagaaatcaa ttatcctgtg cctccaaaat caattaggag
    15781 ctctgtaaac cagagtcctt ctcgacccgt cgcataaatt tactttaaca cagttcctat
    15841 gtttatgcga gagctttttt cattttataa tcgaccattt gaaatccttg accgatatct
    15901 gaaataagtt cttctgtaat ctcaaatcct tcatgcttat aaacctcgat tgcttgatga
    15961 ttcccctgat tcactcgcaa aaagatctct ctttcctttg tttgcgcagc aagttcttca
    16021 taccagcgaa aaactgaaga agtcaatcct tgaccacgta ctttatttag caaatataat
    16081 ttacttaaat aaatttttcc atttatctct tcataagcag tgtaaccgac agtcttttct
    16141 tcgcttttta ataaaaagta ggacacacct tctgtcagtt ctttttgaat ctgttttttt
    16201 gactgataag ttttcagcat gtaatccact tgtttttgac caatgatcgg cacaaaaact
    16261 tccggccata cttcttgtat gactgccatc agctcatcca gttcattttc tgttactttt
    16321 attttttcca tacacccacg tcctttttta cattataaat gaccttgcgc ttgatggaat
    16381 aaaaagagca tgatcatgct atacacaccc accgaccacg gtttcaagaa aatcacaatc
    16441 tgcataaatt ccctaaaaga taatttcgtc aaaccagcta ctagacagat caagtcgtct
    16501 ggcgcaaagg gaagcagcaa ggtaacaact agtacttttt taatatcttt cgtttttctt
    16561 gtcagcaatt catcaaattt ctgatacttt tttggcgata aaataagctg gacaaaggct
    16621 acgccatagt aacggacaag caaaaatccg attgcttccc caatcactag gccaatacaa
    16681 ctatataaaa gcccaaatcc attgccaaac atcagcatgc ccgctactga ggagatccct
    16741 cccggcaaaa taggaatgat cacttggatc acttgcaaca gaacaaatat catcactgca
    16801 tacttgccaa attgctggat aaacgcttgc aaagacgtgc tcgatcgaaa aatcccatga
    16861 tgataagcat aaagaatcaa cacaaagaaa aaaaacagac caataatggg catgatctga
    16921 agacattttt gtaaaactga tcctttttta gacatctata ttccccacat cttttttcat
    16981 ttattaaaac tgcctcct
//
LOCUS       DAZBTF010000060        16714 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000060 DAZBTF010000000
VERSION     DAZBTF010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.060"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(251..1462)
                     /locus_tag="ACHGNS_002065"
     CDS             complement(251..1462)
                     /locus_tag="ACHGNS_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN6937992.1"
                     /translation="MSEVTGYLEQVDNETYRLRAVLGYKPDGSAKRKSKTIKASSKRA
                     AYKELNIWLEQFEGMTDDSLDLFNITFGEFYRKIWLSEAEKNLEPKSYHNYKRMIENR
                     FLDKFDFIPLIDIKPYMIKKIVVNAQRINTKDPGRNSDKPLSRNTKLRMLYAVNNLFL
                     MAKNEYGAIKENPVENVKIPKEKGVKKNIEEPYSEEEIHAMLKAAFEESIEIKTLIVL
                     AFITGARQGEIAALEEKDIDFDKQEIRFHQRISEVDGKSDIRLLPGLKNDDDEKIVTG
                     PAYLFDMLDELIKENKKIRWKLNIKKLKHYFIFDTKQDGTLPRGSYLYKKFKRFTKRH
                     NLRHIRFHDIRHTSATYLLSDPNMTPKELQKRLGHRDFNTTMNVYGHVLRKEKDTATT
                     AFENLLKKDIK"
     gene            complement(1581..2216)
                     /locus_tag="ACHGNS_002066"
     CDS             complement(1581..2216)
                     /locus_tag="ACHGNS_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFN6937993.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWVLIVLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTTKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAKNNVLQAQTGYFGTETVSIALHN"
     gene            complement(2352..3005)
                     /locus_tag="ACHGNS_002067"
     CDS             complement(2352..3005)
                     /locus_tag="ACHGNS_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937994.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDATSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQVTAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFFKYGIQ"
     gene            complement(3108..3314)
                     /locus_tag="ACHGNS_002068"
     CDS             complement(3108..3314)
                     /locus_tag="ACHGNS_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HFN6937995.1"
                     /translation="MPSYVVLQVVLKEKFIGKGSQNLSELENTINRQCSKGYRLHTIS
                     TTNGGSKGFGGGDRIQATLVFESL"
     gene            complement(3390..4136)
                     /locus_tag="ACHGNS_002069"
     CDS             complement(3390..4136)
                     /locus_tag="ACHGNS_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004564412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFN6937996.1"
                     /translation="MKQTTQQRLNQLMSERNLKQVDILNMSLPLQKETGIKMSKSHLS
                     QYVNGKSSPDQHKLYLLAKTLNVSEAWLLGYDVPKEDKKNNIPSIETIYNQLEKPRQT
                     KVYNFAEQQLEEQNRGNVVHFPKKEKLPTIKNSASAANPTELVYGDTVVEEEEFERVP
                     NNADFAVPIIGDSMEPVIRNGQFVFVKEQPDVEDGEIAIVEIDGDGVTCKEVYKDYES
                     QTIILRSINELYEDRVVSPEQIRIIGKVVF"
     gene            4296..4520
                     /locus_tag="ACHGNS_002070"
     CDS             4296..4520
                     /locus_tag="ACHGNS_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF739 family protein"
                     /protein_id="HFN6937997.1"
                     /translation="MSFDYSKLSGRIVEKYGTQYNFSIALGLSERSLSLKLNNRVPWK
                     STELQKAIELLDIPKEEIGEYFFNLEVQNV"
     gene            4544..5305
                     /locus_tag="ACHGNS_002071"
     CDS             4544..5305
                     /locus_tag="ACHGNS_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HFN6937998.1"
                     /translation="MNTQKFENNLFELEVKTENGESLFNAETVARSLGFVETKNGKEY
                     VRWRTVNGYLKKYLSQEVAKNDFISEPMVYKLAFKANNVLAEKFQDWLATEVLPQIRK
                     HGMYATDELLNNPDLLIEVATKLKEERTLRLIAEQRVNELQPKADYYDRILNNKGLVT
                     VSTIAKNYGMSAVSFNKLLHELGIQFNQSGTWLLYSKFQDKGYTHIEPFDYEDKNGNR
                     QVKTRMKWTQKGHIFLYETLKKNNYLPMIEREQTA"
     gene            5317..5574
                     /locus_tag="ACHGNS_002072"
     CDS             5317..5574
                     /locus_tag="ACHGNS_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6937999.1"
                     /translation="MNEEMSQTIKVQKMIDDLTHAIDSQADKIIKELQGQKVWDAKML
                     FKTIKFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            5666..5917
                     /locus_tag="ACHGNS_002073"
     CDS             5666..5917
                     /locus_tag="ACHGNS_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HFN6938000.1"
                     /translation="MEQLALVNLSDLKASLAESEIANEVWDTKQAAEYLKTTTRTLTK
                     DAESGKIPAAKVGREWRFSSIALYQYLKGGKTWVNSTEH"
     gene            5893..6066
                     /locus_tag="ACHGNS_002074"
     CDS             5893..6066
                     /locus_tag="ACHGNS_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938001.1"
                     /translation="MGKFNRALVFSAPLIVYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEYRAKKPTEGAD"
     gene            complement(6180..6587)
                     /locus_tag="ACHGNS_002075"
     CDS             complement(6180..6587)
                     /locus_tag="ACHGNS_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938002.1"
                     /translation="MSFDNTITISIILALVALISPSITAVINNKHVESMKDKEIELQK
                     HDSKTQTIQTTFSTFLNNVGICIGSNTDKNISAVKASGYAVLPYIQNEDIEVMKIFLS
                     RFGYGNTDAEQKSLETYLIDKVLPILNKSLEKL"
     gene            6748..6972
                     /locus_tag="ACHGNS_002076"
     CDS             6748..6972
                     /locus_tag="ACHGNS_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938003.1"
                     /translation="MISVKGLGDEIFEAMMSKAQQDIKEKIISAAAQGQTSCTVRSRG
                     TTPSFLLALEDEGILNIKRRDGSVRLFWQF"
     gene            6982..7176
                     /locus_tag="ACHGNS_002077"
     CDS             6982..7176
                     /locus_tag="ACHGNS_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938004.1"
                     /translation="MKDFDSLGARQQPPNEASPVGVDWQENPLYPGDTCYLTEEGYVP
                     VDAILEYVQQHYPKIELGGI"
     gene            7179..8201
                     /locus_tag="ACHGNS_002078"
     CDS             7179..8201
                     /locus_tag="ACHGNS_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFN6938005.1"
                     /translation="MANDLTQTTQRSLDEQVISNLGRLQEQGLEMPPGYSPQNALKSA
                     FFELTNNTGGNLLQMAANNQEMKTSISNALLDMVIQGLSPAKKQCYFIKYGNKVQLMR
                     SYFGTMAVLDRVTGGADITPVVVRQGDEFEVAMDGPNMVVKKHETKFENLDNEIIAAY
                     VVIKLTNGKETTTVMTKKQIDQSWAKSKMKDSGPQKEFPEEMAKRTVINRAAKTLINT
                     SNDNDLLVQAAKDTLENEFDNDRKDITPQTEKVATLEQKFFSNKKITEPIQKEPDPIV
                     IPDDIQNEVTRVADVPAHPEIEQAHLIENEDINPIQEELIDIPDFGREEGVEDVSEFE
                     DDEYPF"
     gene            8164..8979
                     /locus_tag="ACHGNS_002079"
     CDS             8164..8979
                     /locus_tag="ACHGNS_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HFN6938006.1"
                     /translation="MSQNLKTTSTLSDENYYSNEADWQYMSTSQYKSFLKCEAAALAK
                     LKGDWAPTSDPKALLVGNYVHSYFESKEVHEAFKEENKSKMFSSRKPYGLLKDFQIAE
                     QMIERLKQEEAFMNIYQGEKETIVTGELFGTTWKGKIDCLNVEDEYFVDIKTTKDMHE
                     RKWNENYGSRETFIVNFGYVLQMAIYQELLLQQYGKMFMPIIAAVSKQTPSEAKLITI
                     DQDKMSYELIMLKENIERIVKVKNGEERPIPCGTCEYCRGNLSITGFTSMDDL"
     gene            8997..9779
                     /locus_tag="ACHGNS_002080"
     CDS             8997..9779
                     /locus_tag="ACHGNS_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938007.1"
                     /translation="MNLGYIKLYRKVTSSFVWTNSDMFKLWILCLMKASHEDRKFLFN
                     GQEVRLTSGQFVTGAHAIAKEYNEGVPSDKAIAWRTLWRWLKKFENEELLTIQSNARY
                     SVITIINWSDYQSSDKPVTSDGQSSDKPVTTNKNDKNDKNDKNNNPRNSRKTREYADD
                     DPNKKLAILLLKLIRKNRSLKEPDLNKWANTIRLTIEADKRTGKEVQDMIVWATNHDF
                     WSTVISSPTSLRKNFDTMVGQKNKRKQQNISNDELPETGEDW"
     gene            9779..10627
                     /locus_tag="ACHGNS_002081"
     CDS             9779..10627
                     /locus_tag="ACHGNS_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN6938008.1"
                     /translation="MNKKLSAMAAPYGGLKTADHNCPKCGDPLYIWKTKNKDGTDRCG
                     PTCINKICGYREMVTKNQKEAIQKANEAMKKDAINRMLNSSMITDDAIWTFDFDGYKV
                     VDQETAQIKAMAQEWAKKIVNGSTIHAVITGRTGAGKTHLGAAVIKEVMRASNYKIAC
                     SFISYRELLEQLKFAMNDPEARKAVTGSLMAEIKKSDFVVIDDLGAELGRMEENNQAT
                     PYDVDVLTSLTEARLNKATIFTTNLSSKQLKHAYGERVFSRVMNGTKGNIAVFKTTTD
                     KRRNPV"
     gene            10624..10866
                     /locus_tag="ACHGNS_002082"
     CDS             10624..10866
                     /locus_tag="ACHGNS_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938009.1"
                     /translation="MTFVVKKMCYLDSRGRGEASVEFAKHHTTKEEADLVASVCGGEV
                     VEVIKPKRRFSKPKTKPVKKECCCPKSNQAWMRGAK"
     gene            11009..11377
                     /locus_tag="ACHGNS_002083"
     CDS             11009..11377
                     /locus_tag="ACHGNS_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFN6938010.1"
                     /translation="MATRSKYGNKKHEVDGITFDSITEAEYYDLALWQAQANGWKVKL
                     QERFELMPKFELEGKKYRKIEYIPDFTFYKSGKLVKVVDVKGMQTKDFKIKAKLFCHQ
                     YQVPLILAKKYRNTFKEERF"
     gene            11392..11553
                     /locus_tag="ACHGNS_002084"
     CDS             11392..11553
                     /locus_tag="ACHGNS_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFN6938011.1"
                     /translation="MTTEEVIQMRIRSLRREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            11550..11876
                     /locus_tag="ACHGNS_002085"
     CDS             11550..11876
                     /locus_tag="ACHGNS_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938012.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            11873..12688
                     /locus_tag="ACHGNS_002086"
     CDS             11873..12688
                     /locus_tag="ACHGNS_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFN6938013.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            12690..12815
                     /locus_tag="ACHGNS_002087"
     CDS             12690..12815
                     /locus_tag="ACHGNS_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938014.1"
                     /translation="MGFFMAKILLFLGLVGAAYLVYALLSQTDDKEDDNNDEMKF"
     gene            12827..13138
                     /locus_tag="ACHGNS_002088"
     CDS             12827..13138
                     /locus_tag="ACHGNS_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFN6938015.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEGS
                     K"
     gene            13135..13380
                     /locus_tag="ACHGNS_002089"
     CDS             13135..13380
                     /locus_tag="ACHGNS_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938016.1"
                     /translation="MIKKLIQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            13377..13793
                     /locus_tag="ACHGNS_002090"
     CDS             13377..13793
                     /locus_tag="ACHGNS_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFN6938017.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIYENSELLEVE"
     gene            13790..14128
                     /locus_tag="ACHGNS_002091"
     CDS             13790..14128
                     /locus_tag="ACHGNS_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938018.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMYGNSESAIKLFGAIEIYADDF
                     PNLWLKEEAE"
     gene            14125..14355
                     /locus_tag="ACHGNS_002092"
     CDS             14125..14355
                     /locus_tag="ACHGNS_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938019.1"
                     /translation="MKYEIPLSEADVQSIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSINDTFRLGS"
     gene            14362..14565
                     /locus_tag="ACHGNS_002093"
     CDS             14362..14565
                     /locus_tag="ACHGNS_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFN6938020.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASDAIRKFGISILSDDRKWEEKE"
     gene            14562..14858
                     /locus_tag="ACHGNS_002094"
     CDS             14562..14858
                     /locus_tag="ACHGNS_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938021.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKWTAKSKENLAVVRLVDMELINDK"
     gene            14935..15348
                     /locus_tag="ACHGNS_002095"
     CDS             14935..15348
                     /locus_tag="ACHGNS_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFN6938022.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDALIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            15361..15747
                     /locus_tag="ACHGNS_002096"
     CDS             15361..15747
                     /locus_tag="ACHGNS_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938023.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
     gene            16298..16525
                     /locus_tag="ACHGNS_002097"
     CDS             16298..16525
                     /locus_tag="ACHGNS_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938024.1"
                     /translation="MRTYWYVSLNNKYPKPMKGQHRRVVMSVQMKAKYSIVEMLREAT
                     PKEIDQYKLVLIGIGWFKDEHIQTNMKRWLR"
     gene            16522..>16714
                     /locus_tag="ACHGNS_002098"
     CDS             16522..>16714
                     /locus_tag="ACHGNS_002098"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938025.1"
                     /translation="MKLYYVEKANCWVVEGCLSKYATDIISTQFSELGLKVIFCGEFV
                     SVKEAVLPMTAKSRRSRGSS"
BASE COUNT         5664 a         2726 c         3485 g         4839 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtt
      121 cgttatttaa tggtatttta aatgaagata agcgaattta cagcattttt aaatgcttat
      181 ataccgcaaa ttatcccaag tttttgtata tcctacccga gtttataccc gaaaatataa
      241 ttttttagtg ttattttata tcttttttta ataggttttc aaacgctgta gttgctgtat
      301 ctttttcttt gcgtaagaca tggccataaa cattcattgt agtattaaaa tctcggtgtc
      361 ccaatcgttt ttgaagctct tttggagtca tattaggatc gctaagcaaa taggtagctg
      421 atgtatgtct aatgtcgtga aaacgaatgt gacgcaaatt gtgacgtttg gtgaatcgtt
      481 tgaacttctt atacagataa cttccacgtg gcaaagttcc atcttgtttt gtatcaaata
      541 taaaatagtg ttttaatttc tttatattta atttccaacg aatcttttta ttctctttga
      601 ttaattcatc taacatatca aatagataag caggaccggt tactattttt tcatcatcat
      661 catttttcaa acctggcagc aaccgaatat ctgattttcc atccacttct gaaatccgct
      721 gatgaaatct tatttcttgt ttgtcaaagt caatatcttt ttcttctaaa gctgcaattt
      781 ctccttgtct tgctcctgta ataaatgcta gaacaattaa agttttaatt tctatgcttt
      841 cctcaaatgc tgcttttagc attgcatgaa tttcttcttc cgaataaggt tcttcgatat
      901 tcttttttac gcctttttct tttggtattt ttacattttc cacaggattt tctttaattg
      961 ctccgtactc atttttagcc atcaaaaata agttgttaac ggcgtataac atacgaagct
     1021 ttgtatttct tgataaaggt ttatcagagt ttctcccagg atcttttgta ttgattcttt
     1081 gagcattaac aacaattttc tttatcatgt atggtttgat atcgattaga gggataaaat
     1141 caaatttatc aagaaatcta ttctcgatca ttcgcttata gttatgataa gactttggct
     1201 ctaaattttt ttcggcctct gaaagccata tttttctata aaattcacca aaagttatat
     1261 tgaacaaatc caacgaatca tcggtcattc cttcgaattg ctctaaccag atgttcaatt
     1321 ctttatatgc cgctctttta ctacttgctt ttatagtttt gctttttctt tttgccgagc
     1381 catcaggttt gtatcctaat acagctctta gtctatatgt ttcgttgtct acttgttcta
     1441 agtatcctgt tacttctgac ataattgaat cagctctctt tctctgatac aataggtact
     1501 ataaagaagc ctatcgtata ggtttgtttc tttcttagaa cacgctcgct ttggtcggtg
     1561 gggcgtgttt tttatactaa ttagttgtga agagcaattg aaactgtctc tgttccaaag
     1621 taacctgttt gtgcttgcaa tacattattt tttgcatttg cttgggcttc agagatatca
     1681 aaaactattt taccagtttg ttgcatatca ggatttaagc tttccataaa gaaagagtta
     1741 gtgatattac cattttcatc ttgatttgcc gacatagatg cagccgaatc agcttcgaaa
     1801 gtttttccat ctgcttttaa tttgaagaat gagctatcaa cagtcacagc cttatcacct
     1861 gcatttttta cagataagtc aactactaag aaagtgtctt ttgcgttagt aggaagtaca
     1921 gatgggccaa cttgttttac tacttcaacg gagtttactt tgtattccat tttccctaca
     1981 gcaacgtcat caccgatttt ataagtcttt tcttcttttg ttgtagtttc ttttgtagtt
     2041 tctttattag tacttgttga tgttgcagta gctgaatctt tgttttcacc gccacttaga
     2101 gcaccaccaa taccaaaaaa caaaagtact attaataccc aaaaccatac ccgtttataa
     2161 aacggttttt taaccttata cattttccca tcttgaccca taactttttt tgccatttaa
     2221 atattcctca tttcttgtta taatatattt gtgatctcag aaatgaggta tgagtccgtg
     2281 ttgcagcacg ggctttttta tttataactt ttttagagat tataggcaaa atagtagggc
     2341 ataaaaatat attattgaat tccgtattta aaaaatccta attgaacttc accggaagtc
     2401 ttttgctgtg ttgtacgcaa tgcttcttct tcagacattc cattctgtac tttccatgca
     2461 acaggcgaca tcccgtattt gttaacaaaa tcagtaagtg ataaagtgtc agcgtcttgc
     2521 tgagcgcttg tttgttggtc ttctggattt tgttgagatg ctgcttgttg ttctttctga
     2581 tcttgactga taatattgcc agcatcatct gtagtcaatc cattttcata aagggccacg
     2641 ccgaaagctt cccactcttt gttggaccaa tttgcacgat cagctggagt tgactgtaaa
     2701 gtgcgttgtt tcatctgttc atatgtttct tcttgaggtg cggtttggat tgtactctga
     2761 ctggagctta taactgttgg gctaggttcc gccgtaactt ggttggagct tgtaactgtt
     2821 gaactagaat ctgttttaga tgtagatttg ctagtagatg aactggtttc agttgtttct
     2881 tttgttttac ttatttttgt ttcttggtta gaagtggcat ctgttgattc agctttttta
     2941 ttatttgaac aagctgaaag tagcagagca gtacttaaca acaacataac gctaactttt
     3001 ttcattttat actcctcttt tctttgatac aataagttgt aaagaagcct actataggtt
     3061 taacatccca cgtccacaaa ctttggtcgg tgggcgtgtt tttttgttta tagactttcg
     3121 aaaactaaag tagcttggat tctatcgcca ccaccgaaac ctttactacc accgtttgta
     3181 gtggaaatag tgtgtaatcg ataaccttta gaacactgtc tatttatagt gttttcaagt
     3241 tctgataggt tttgcgaccc ttttcctata aacttttctt ttaatacaac ttgcaataca
     3301 acgtaacttg gcatattctt ttccttcttt ctactatgat aataaatact aacccctagc
     3361 cggagcgcca actacgactg gggtattttt tagaatacga ctttcccgat tattctaatc
     3421 tgttccggtg agacgactct atcttcatat aattcgttta tagagcgtag aataatcgtt
     3481 tgactctcat aatccttata tacttcttta cacgtcacac cgtcaccatc tatttcaacg
     3541 atagcaatct caccgtcttc cacgtctggc tgttccttaa caaatacaaa ttgtccattc
     3601 ctgataactg gttccattga atcgcctata ataggaacag caaaatcagc attgtttgga
     3661 actctttcaa attcttcttc ctctacaact gtatcgccat aaactaattc agtcgggttg
     3721 gcggcagaag ctgaattttt gattgtcgga agtttttcct ttttaggaaa gtgcaccacg
     3781 tttcctcgat tttgctcttc gagttgctgt tcagcaaagt tgtagacttt tgtttgtctt
     3841 ggtttttcta attgattgta gattgtttca atggatggaa tgttattttt cttatcttct
     3901 ttaggaacat cgtaaccaag caaccatgcc tcactcacat ttaaagtttt agccaacaaa
     3961 taaagcttgt gttggtcggg cgatgactta ccattgacat attgcgatag atgacttttt
     4021 gacattttta ttccagtttc tttttgcaaa ggtaaagaca tattcaaaat atcaacttgc
     4081 tttaaatttc tttcagacat taactgatta agtcgttgct gagtcgtttg cttcatctta
     4141 tacctccttt ctgatactta ttatatacct tattgaacat aagttcaata aaaaagttaa
     4201 attaatttga acttttatgt tgacaatgtt ttatgcagga gttatacttt ggttacaaag
     4261 ttcaaaacat ttgaactaga aaggagcgaa ctaatatgag ttttgattat tctaagttgt
     4321 cgggaagaat tgttgagaaa tatggaacgc aatacaattt ttcaatcgct ttaggtttgt
     4381 cggaaagaag tttgtcatta aagttgaata accgagtacc atggaaaagc accgaacttc
     4441 aaaaagccat tgaattgcta gatattccaa aagaagaaat tggcgaatat ttttttaatt
     4501 tagaagttca aaatgtctga acttaagaaa ggaattcgaa aaaatgaaca cacaaaaatt
     4561 tgaaaacaat ctatttgaat tagaagttaa gacggaaaat ggtgaatcgt tatttaatgc
     4621 tgaaacagta gcaagaagtt tgggtttcgt ggaaacaaaa aacggtaaag aatatgttcg
     4681 gtggagaact gtcaatggat atttaaaaaa atacctttcg caagaagttg cgaaaaacga
     4741 ctttatcagt gagccaatgg tttacaaatt agcctttaaa gcaaacaacg tacttgctga
     4801 aaaattccaa gactggttag caacggaagt cctacctcaa atccgaaaac acggcatgta
     4861 cgcaacagat gaactactta acaatccaga cttgctgatt gaagtagcaa ccaaactcaa
     4921 agaagaacgc acgttacgat tgatagctga acaacgagta aatgaattac aaccgaaggc
     4981 agactattac gaccgaatct tgaacaacaa aggattagtt acagttagta cgattgctaa
     5041 gaactacggt atgagcgctg tatcgtttaa taaattgcta catgaattag gtatccagtt
     5101 caatcaaagt ggcacatggc tgctatacag taagttccaa gacaaaggat acacgcatat
     5161 tgagccattt gattatgaag ataaaaacgg caatcggcaa gtaaaaacaa ggatgaaatg
     5221 gacgcaaaaa ggacacatct tcttatatga aacattgaag aagaataatt acttaccgat
     5281 gattgaacgt gaacaaacag cataggaggt aacactatga acgaggaaat gagccaaaca
     5341 atcaaggtac aaaaaatgat agatgatcta acacatgcaa ttgatagcca agccgataaa
     5401 atcatcaagg aattgcaagg acaaaaagtt tgggatgcaa aaatgctttt caagactatc
     5461 aaatttgaaa tgaatccaac aaaaagaaag ctcgccgatg tgttggaaga aaagttggct
     5521 tccgctatca acgagcaaga attactattt gaaaccgata cttttaacag ctgatgtttt
     5581 atagatgaca gtttcatccg aatctatatc attatagatt cgattattgc aatgtacgaa
     5641 ttataaatac aggaggaaat aaccaatgga gcaactagct ttagttaacc tctcagacct
     5701 taaagcttct ttggcagaat ctgagattgc aaatgaagtg tgggatacaa agcaagctgc
     5761 ggaatattta aaaaccacaa cacgcacgct gactaaagat gccgaatctg gaaagattcc
     5821 agcagcgaaa gttggcagag agtggaggtt tagtagtatc gctttgtatc agtatctaaa
     5881 aggaggaaaa acatgggtaa attcaacaga gcactagtgt tcagcgcacc cctaatcgtc
     5941 tatgctttag gactttgggg aagcagacaa gcattaatcg gcacgatcgt ttacatggtt
     6001 tggattttta tggggcttga tgaagctgag tacagagcga aaaagcccac cgagggggct
     6061 gactaaagtg tggttctgtt tattaggtgt ttatctcgtg gccgttttag taaataacta
     6121 catgagaaaa cgtggcgaat actggtatac atcctatgcg gttctagtat gtctaatgct
     6181 tacaattttt ctaatgattt attcaaaata ggtaatactt tgtcaatcaa ataagtttca
     6241 agagattttt gttctgcatc agtgttgccg taaccaaaac ggcttaagaa aattttcata
     6301 acctctatat cttcattttg aatatatggc aggactgcat aacctgatgc ttttacagca
     6361 gatatatttt tatcagtgtt actaccaata caaataccca cattgttgag aaatgttgag
     6421 aaagttgttt gtattgtttg ggtttttgaa tcgtgttttt gtaattcaat ttctttatcc
     6481 ttcatcgatt caacatgctt attatttata accgctgtaa tcgaagggga tataagagct
     6541 actaaagcta ggataatcga aattgtgatc gtgttatcga aactcatttt ttcaccatcc
     6601 agtttttaaa ccattatatc aaaaaggaga gaagaaataa tgcaagaatt agtaattttg
     6661 aaaaataaaa aaagacacga acgccagcaa gcatgatcgt gtccaaaaga aaaatctttt
     6721 aaaccattat aacacaggag gaaataaatg atctcagtta aagggttagg cgatgaaata
     6781 ttcgaagcaa tgatgagcaa agcacaacag gacatcaaag aaaaaatcat ttctgctgca
     6841 gcgcaaggac aaactagctg cactgttcga tcgagaggaa cgacaccaag ttttcttttg
     6901 gcgctagaag atgaaggcat tttgaatatc aaacgaagag atggcagcgt cagattgttt
     6961 tggcaatttt aggaggccgt gatgaaagat tttgattctt taggcgccag acagcaacca
     7021 ccaaatgaag ctagtcctgt tggagttgat tggcaagaaa atccgttata tccaggtgat
     7081 acttgctatc taacagaaga aggctatgta ccagtagacg caattctaga gtacgtccag
     7141 caacattatc caaagattga attaggagga atttaggaat ggcaaatgat ttaacacaaa
     7201 caacacaacg ttcattagat gagcaggtaa tcagtaatct tggacggtta caagaacaag
     7261 gtttagaaat gccaccagga tatagtccac aaaatgcgtt aaaaagtgct tttttcgaac
     7321 tcactaacaa tacaggagga aacttgttgc agatggctgc aaacaatcaa gaaatgaaaa
     7381 cctccatttc taatgcactt ttagacatgg ttattcaagg gttatctcca gcaaaaaaac
     7441 aatgctattt cattaaatac ggaaacaagg ttcagctaat gcgttcatat tttggaacaa
     7501 tggcggtcct tgatcgtgta actggagggg cagatattac gcctgtagtc gttcgacaag
     7561 gtgatgagtt tgaagttgca atggatggac caaacatggt tgtcaaaaag catgaaacaa
     7621 aattcgagaa tttagacaat gaaatcattg cagcctatgt agtcatcaag ttaacaaatg
     7681 gtaaagagac cacaacagtc atgacgaaaa agcaaatcga tcaaagttgg gctaagtcaa
     7741 aaatgaaaga ttctggaccg caaaaggaat ttccagaaga aatggctaaa cggacagtaa
     7801 tcaatcgggc agctaaaaca ttgatcaaca caagcaacga caacgattta ttagttcaag
     7861 ctgcaaaaga tacgttagaa aatgaatttg acaatgatcg gaaagatata acgccacaaa
     7921 cagaaaaagt ggctactctc gaacaaaaat tcttttcaaa caaaaagatt actgaaccaa
     7981 tacaaaaaga accagatccg attgtgattc cagatgatat ccaaaatgaa gtaactcgag
     8041 ttgctgatgt acctgcccat ccagaaatag agcaagcaca tttgatcgaa aatgaggata
     8101 tcaatccaat tcaagaagaa ttgatagata ttccagactt tggacgcgag gaaggtgtag
     8161 aagatgtctc agaatttgaa gacgacgagt accctttctg atgagaatta ttactccaat
     8221 gaagctgact ggcagtacat gtcaacatca caatataagt cctttttaaa atgtgaagcc
     8281 gcagccttag caaagttgaa aggcgattgg gcaccgacgt cggatcccaa agccttactc
     8341 gtaggaaact acgtacattc ttattttgaa tcaaaagaag tacatgaagc gttcaaagaa
     8401 gaaaataaat ccaaaatgtt ttctagtagg aagccgtatg gattgttgaa agatttccaa
     8461 attgctgaac aaatgattga acgacttaag caagaagaag cattcatgaa catttatcaa
     8521 ggtgagaaag aaacgatcgt gactggggaa ctatttggaa cgacatggaa aggcaagatt
     8581 gattgcttga atgttgagga tgagtatttt gttgatatca aaacaaccaa agacatgcat
     8641 gagcggaaat ggaacgaaaa ctacggatca agagaaacgt tcattgtcaa ttttggttac
     8701 gtacttcaaa tggcaatcta tcaagagctt cttttacagc aatacggaaa gatgttcatg
     8761 ccaatcatcg ctgcggtatc gaagcaaacg cctagtgagg cgaagttgat aacgattgat
     8821 caagataaga tgagctacga gctaatcatg ctaaaagaaa atattgaaag aattgtcaaa
     8881 gtgaaaaatg gcgaagaaag gccgatacct tgcggtacgt gcgagtattg tcgaggtaac
     8941 ctttcaatta ctggattcac tagcatggac gatctttaaa aggaggtgct ttgcgaatga
     9001 atctaggata catcaagtta taccgtaaag tgactagttc attcgtttgg accaattccg
     9061 atatgttcaa gctatggata ttgtgtttaa tgaaggctag tcatgaagat agaaaatttt
     9121 tatttaacgg tcaagaagta cgtttgacaa gcggacaatt cgtcacagga gcccatgcaa
     9181 tagcaaaaga gtacaacgaa ggagtcccga gtgacaaagc aattgcatgg cgaacgctat
     9241 ggagatggct taagaaattt gaaaatgaag aattattgac aatccaatcg aacgctagat
     9301 atagcgttat aacaataata aattggtctg actatcaatc gagtgacaag ccagtgacaa
     9361 gcgacggaca atcgagtgac aagccagtga caacaaacaa gaatgataag aatgataaga
     9421 atgataagaa taataatcct cgcaactctc gaaaaacacg agagtatgca gatgacgatc
     9481 caaataaaaa attggccatt cttttattaa aactcattcg aaagaatcgt agtctcaaag
     9541 aacccgattt gaacaaatgg gccaatacga ttcgtttgac gattgaagct gacaaacgaa
     9601 ctggaaaaga agttcaagac atgattgttt gggctactaa ccatgatttt tggtccacag
     9661 tcatttcatc gccaactagc ttaagaaaaa atttcgatac aatggtgggt caaaaaaata
     9721 aaagaaagca acaaaatatt tctaatgatg agttaccaga aacaggtgag gattggtaat
     9781 gaataaaaaa ctaagtgcaa tggctgcacc ttacggcgga ttaaaaacag cagatcataa
     9841 ttgcccgaaa tgtggtgatc cattgtatat ctggaaaaca aaaaataaag atggtactga
     9901 tcgatgcggt cctacatgta tcaataagat ttgtggttat cgagaaatgg taactaaaaa
     9961 tcaaaaagaa gctatccaaa aagcgaatga agcaatgaaa aaggacgcta tcaatcgaat
    10021 gcttaacagt tcaatgatta cagatgatgc catatggacc ttcgattttg atggatacaa
    10081 agtagttgat caggaaacag cacaaataaa agcgatggct caagaatggg ctaaaaaaat
    10141 tgtaaatggt agcacgattc atgcagtgat aaccggtaga acaggagccg gcaaaaccca
    10201 tctaggcgct gcagtgatta aagaagtcat gagggcatct aattataaaa ttgcttgttc
    10261 atttatcagt tatcgagaac ttttagaaca gttgaaattt gcaatgaatg atccagaagc
    10321 aagaaaagcc gtaacaggtt cgctgatggc tgagataaag aaatcagatt tcgtggtaat
    10381 cgacgatttg ggtgcagaac taggaagaat ggaagaaaac aaccaggcaa cgccttatga
    10441 tgttgatgtt ctcacatcgc tcacagaagc tcgtctaaac aaagctacga tattcacaac
    10501 caatttatca tcgaagcaat taaaacacgc gtacggtgag cgagtgttct ctcgtgtaat
    10561 gaatggaacg aaagggaaca tagctgtatt caaaacaacg acagacaaaa ggaggaatcc
    10621 agtttgacct ttgtagtgaa aaaaatgtgc tacttagata gccgagggcg aggagaagct
    10681 agtgtcgagt ttgctaaaca ccacacgact aaagaagagg ctgaccttgt tgcaagtgtt
    10741 tgtggcggcg aagtagtcga agttattaaa cccaaaagac gattttcaaa acctaagaca
    10801 aagccagtga aaaaagagtg ttgttgtccg aaaagtaacc aagcatggat gagaggtgca
    10861 aaatgacttg tttaaaatgc aacgatgaaa cagttatttg gtataagaca ttgcttggat
    10921 ggtcaacttg tgaaccttgt ccaatatgca atgaaaatgg acgacgttcg aaagaacgac
    10981 tcgaaagact aaagaaggag tatagcaaat ggcaacaaga agcaaatacg gaaacaaaaa
    11041 gcacgaagta gacggcatca cttttgattc tatcacggaa gcagagtatt acgatctagc
    11101 cttgtggcaa gcacaggcga atggttggaa agtgaagctc caggaaaggt ttgagctgat
    11161 gccgaagttt gaactagagg gcaagaagta tcgcaagatc gagtatattc ccgacttcac
    11221 attttacaaa agcggcaagc tggtcaaagt cgtagatgtt aaggggatgc agacaaaaga
    11281 ctttaagatc aaggcaaagt tgttctgtca tcaatatcaa gtgccgttga tattagccaa
    11341 aaaatatcgg aatacgttca aggaagagcg tttttaacga ggtggtccat catgacaaca
    11401 gaagaagtga ttcaaatgcg aattcgaagc cttcggcgtg agattgacga tctggaacga
    11461 acaaaggcag tgatggtcaa tgaaacggct agaaaggcaa tcgatttgca catagagaat
    11521 ttaagaaggg aaatccgtag attggaggaa tgagcgtgga taaggaagcg gcttggcgaa
    11581 aattaatgtt gctgattcaa gatgagaact ggcaagaaga tgaagcagtg gttgctgaag
    11641 ttcagcgtct aggaaagatt gctaacggac gtatacgaaa aaagccagac aaaagaaaac
    11701 agcgcaaagg gaaaatcgtc gttgttttac acgaaggcaa aattttgatg caaggaacag
    11761 ctagtgagct gtctgcagaa actggatata cgcgtgggac tattcgaacg tacgcttggc
    11821 gaaatcacac cgatcgaaaa gggcacgaat ataagtattt ggaggaagaa aaatgaacga
    11881 aaacaaatta atcaaattgg gtgtagcagg agcagtaatc gtaggtattg gagttatcgg
    11941 aggatttaag ttcttcgaaa aaatcgataa tggatatgtg ggtgtgcgct attcaatgaa
    12001 cggcggtatc aaagatgaag cactgacgca aggtgtgaaa tttgtaggga ttgacaaagt
    12061 gatccaatat ccaattcgct tgcaaactat ccaatcaaaa aatatttcag tatctacaag
    12121 cgacggcaaa aagacaacga ttgatatcaa atatgactac aaagttgatt caactaaagc
    12181 agcaaaaatg tacaaggaat ttgggaatat cacttcggaa gatatcgaaa gtggatggtt
    12241 aaaatctaag cttcaaaagg tcgctcgtga agtttatgcg aaatatagtc tgcttgatgt
    12301 cctttcagga gattcctcta aagttgaagc tgaggtatta acgaactttg ctaaatcagt
    12361 tgaatctaaa gggtttgaag tcgaagacgt aacacttggt gttccagatg tcgataaaga
    12421 aacacaaaaa tcaatcgatg cgatcattcg agctggtcaa gaaaatgaaa aagcgaagct
    12481 agacgcagaa actgcaaaaa ctcaagctga tagtgaagct tacaagaaaa caaaagctgc
    12541 agaagcagag gcagaatcta atcgcaaagt cgccgaatca gtaacagaca atttgattcg
    12601 ttatgaagaa gctcaagctc gcaaaaagca tggatgggta acagtaaatg gagcagatac
    12661 tgtagttacg gatgaagcag gcaaataata tgggattctt tatggctaaa attctcttgt
    12721 tcttaggttt agtaggagca gcatatctcg tgtatgcgct cctttcccaa actgatgaca
    12781 aagaagatga caacaacgat gaaatgaaat tttaggagga gaaataatgg acgaactaat
    12841 cacaaaagta gagcagtggg ctaaagacaa gggactggat caagcagatc caaaagcaca
    12901 gtttttgaaa gtagctgagg aattcgggga aatagcttcg gcgatggcaa gaagtaatga
    12961 tgaactattt aaagatagcg taggagacgt tatcgtcacg ctgattatcc tttccatgca
    13021 aaaagggaca aacgtacaag agtgtttaga aatggcatac aacgaaatca aaggacgcac
    13081 agggaaaatg gtagatggtg tattcgtgaa gtcgagtgat ttggagggca gcaaatgata
    13141 aaaaagctca ttcaattcag catggattta tatgatatcg aatcaggagc aacactatct
    13201 gtggaatcgg accatctaat cataaatttt ggtggaaagc gccagattat tttgtgggta
    13261 gttgatgatg tactgtttcc agaaattgtt catgatttcg aagaatcaaa agcggttgag
    13321 tttgaaatag tgaaaaaagt aatggaattg attgaaaaat acgaggagga cagcgaatga
    13381 taccgaagtt tagagcgtgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc
    13441 attttactaa aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga
    13501 aatcatatca cgtgcgaaac atcgacctca tgcaatccac aggaatgaaa gataagaatg
    13561 gtgtggatat atttgaaggg gatatagtat tagtcagcgt gcgaaatggc ttcgattact
    13621 tagataataa agtttgtatt gtcaaaaatt caatagatta ttccggatta gtttgtgcca
    13681 ctgttgatga agacttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt
    13741 atgaagtcat cggaaatata tacgagaata gcgagttatt ggaggtagag tgattatctt
    13801 agctactgac tacatcgatt ctttaaaaga tgaaaatggg aatgtcccag cattgctaaa
    13861 ttgtggaatt aaaagcaaga atagaatcat tttgttacgg atatcggctg atttagcaaa
    13921 atatctttat ctgagcaaga cagcagtata ttacactgcg gctacacgtt atcagtacaa
    13981 gggaaaaaca atcagtcagg attattatga tcgtttaatc agtcctgata tgtatgggaa
    14041 ttcagaatca gcaattaagc tgtttggcgc aatagagata tatgccgatg attttccaaa
    14101 tttatggttg aaggaggaag cggaatgaaa tacgaaatac cactaagtga agcagacgtc
    14161 cagtcaatta ttaacggtcg ggaggttaat aaaaaacttc ctgatggtac tgaattagtc
    14221 atcagacaaa gttatttgaa agatatggca gctccagtat taattgatcg ttttaacgtg
    14281 actgattctg tggtagagaa ccacttgaaa gaatttcgat caagtataaa cgacactttc
    14341 agattaggga gttgattgac aatgaacacc agacaccgca gagtagcaaa actaagaaaa
    14401 caggaactga atgtactaaa gacaaagttt gaaaaagaat atggaatttc agcagaagaa
    14461 acatataaag tggcaagtca gtgtgttgct gatgcaagtg atgctattcg taagtttgga
    14521 atttcgatat taagtgatga tcgtaaatgg gaggaaaaag aatgaaacta aaagacggat
    14581 tttacgctag cagtcacggt atcggcggtt taatgctaga tatgccgaca aagaatccta
    14641 aaacacgtaa gaaaccaaaa gtcaaagtcg gtgacatggt ccgctgtgaa gcagaggagt
    14701 tcatttatcc atttcgtgga tatgtagaac acgtctataa tcactcagcg atcattcgca
    14761 ttgaaaacac gatggaatgt gataaatgga cagctaaaag caaagagaat ttagcagtag
    14821 tgagattggt ggatatggaa ctaatcaatg acaaataaaa aagccggatc gctccgacta
    14881 acataataaa acagacaagt ttattatatc acataaaagg agcagtttga attgatgcaa
    14941 ttgttacgag aggtagattt caaacagaca agatgtaatg cgagagatgt gctgaagaac
    15001 tttcggcgtt tggagcggat ggcaggtcgc tctttgatag atattaagtc gccgattatt
    15061 acggatatgc cgaaggcacc gaagcacggc aataaggcag aggatgcatt gattcagatg
    15121 atggatatag aagcggagag agacgcgatt ttagcggctt tgatggctct tagtctgatt
    15181 agtcgtcaga tactctacta cagtttttgt gtgccagaca gcttctcaaa ctacagaatt
    15241 agccgtgaag tgggttattc agaaagaagt atacaacgga tgaaatcgga agctctaata
    15301 gagtttgcag aagcatataa acacggaaga ataattgctt ataaataggg ggaccatata
    15361 atgtggaaag attatgtatc gttgaaagag ttgaaaaaag atcttgtttt caaaagaatt
    15421 gtagaatggt cagagagtga attaatttta gaagatggaa ccaaaatgga agttgtatgt
    15481 agcgaatcag attgctgcgc atgggccgaa ggtgaattca aaaatgtaaa gcttgatgca
    15541 gtgattacag atataaagat ttttgataaa ggtaaccgtc tctataatgg tgacggacat
    15601 acctcttatg ctgaagtcgt tgtttatcat aatcgtaatg agatcgctaa agcagaatgc
    15661 acagcgaatg atgggaatgg cggctactat tatagtgttt gtgctttaaa ggtcaaagac
    15721 aaactttgta tagtgactga cgcataaaaa aatggcggtt ttttggcgga atgatggcgg
    15781 tttttagcca tttatcagtg atattatggt agtgtcgaaa gattagtgat aggtctgaga
    15841 caaaataata ataaaaggaa catcgtttta ttattgtttc acaattaagc ttcgatagac
    15901 agcaacggaa atattaagaa taaggatgtg aattttaact ccttctaaat tgttcttatt
    15961 atctatcatc cgttgctgtt tattgttaca tacagatcgc ttaggcggtc tttttatttt
    16021 ggccgaaaac ctacattttc gatggccgat tattggagga ggaatagcat gttcaaacta
    16081 tctgaaatca ttaagaagtc cgacgttgag aaattagaga tgttaaaaaa gaagttgaag
    16141 aaatagcagt ctcttcgtga ggctgtttta ttttgctcac aaaaatagac cactatcggg
    16201 taatagtggt caggaattaa atgaaaaaga tgttagggtt gttagctaag tataacatca
    16261 taacgcttac aaaacaatgc aagaaggata aattattatg agaacttact ggtatgtgtc
    16321 attaaacaat aaatatccga agccaatgaa aggacagcat aggcgtgtag tcatgtctgt
    16381 tcaaatgaag gcgaagtatt cgattgtaga aatgctaaga gaagctacac caaaagagat
    16441 tgatcagtat aagttggttc ttattggcat tggttggttt aaggatgaac atattcaaac
    16501 aaacatgaag aggtggttga gatgaagtta tattatgttg aaaaagcaaa ttgttgggtg
    16561 gttgaaggct gtctgtcaaa gtacgctaca gatattatat ctactcaatt ttcagagttg
    16621 ggattgaagg ttatcttttg tggtgagttt gtcagtgtga aagaagcggt attgccaatg
    16681 acagcaaaga gcagacgctc gcgtggttca agtt
//
LOCUS       DAZBTF010000061        15900 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000061 DAZBTF010000000
VERSION     DAZBTF010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.061"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..522)
                     /locus_tag="ACHGNS_002099"
     CDS             complement(52..522)
                     /locus_tag="ACHGNS_002099"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFN6938026.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(681..1139)
                     /locus_tag="ACHGNS_002100"
     CDS             complement(681..1139)
                     /locus_tag="ACHGNS_002100"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFN6938027.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1310..2455)
                     /locus_tag="ACHGNS_002101"
     CDS             complement(1310..2455)
                     /locus_tag="ACHGNS_002101"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFN6938028.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2468..3385)
                     /locus_tag="ACHGNS_002102"
     CDS             complement(2468..3385)
                     /locus_tag="ACHGNS_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFN6938029.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3813..4376
                     /gene="efp"
                     /locus_tag="ACHGNS_002103"
     CDS             3813..4376
                     /gene="efp"
                     /locus_tag="ACHGNS_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFN6938030.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4477..4647
                     /locus_tag="ACHGNS_002104"
     CDS             4477..4647
                     /locus_tag="ACHGNS_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938031.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            4862..6121
                     /gene="lysA"
                     /locus_tag="ACHGNS_002105"
     CDS             4862..6121
                     /gene="lysA"
                     /locus_tag="ACHGNS_002105"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFN6938032.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6108..6908
                     /gene="dapF"
                     /locus_tag="ACHGNS_002106"
     CDS             6108..6908
                     /gene="dapF"
                     /locus_tag="ACHGNS_002106"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFN6938033.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            6911..7792
                     /gene="dapA"
                     /locus_tag="ACHGNS_002107"
     CDS             6911..7792
                     /gene="dapA"
                     /locus_tag="ACHGNS_002107"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFN6938034.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7789..8505
                     /gene="dapB"
                     /locus_tag="ACHGNS_002108"
     CDS             7789..8505
                     /gene="dapB"
                     /locus_tag="ACHGNS_002108"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFN6938035.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8511..9206
                     /gene="dapD"
                     /locus_tag="ACHGNS_002109"
     CDS             8511..9206
                     /gene="dapD"
                     /locus_tag="ACHGNS_002109"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFN6938036.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9222..10223
                     /locus_tag="ACHGNS_002110"
     CDS             9222..10223
                     /locus_tag="ACHGNS_002110"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFN6938037.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10220..11581
                     /locus_tag="ACHGNS_002111"
     CDS             10220..11581
                     /locus_tag="ACHGNS_002111"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFN6938038.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11541..12692
                     /locus_tag="ACHGNS_002112"
     CDS             11541..12692
                     /locus_tag="ACHGNS_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFN6938039.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12667..13836
                     /locus_tag="ACHGNS_002113"
     CDS             12667..13836
                     /locus_tag="ACHGNS_002113"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFN6938040.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            14011..15390
                     /locus_tag="ACHGNS_002114"
     CDS             14011..15390
                     /locus_tag="ACHGNS_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFN6938041.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            15671..15900
                     /locus_tag="ACHGNS_002115"
                     /pseudo
     CDS             15671..15900
                     /locus_tag="ACHGNS_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5202 a         2803 c         3384 g         4511 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tcttttttta tttttattaa gttaaagaac
       61 ggattcaatt gcagcggtca ttttcaatgg agaagtttgt ggtgcaaatc gttcgattac
      121 tttgccattt ctatcaatca aaaatttagt aaaattccat tttattgctt tagtgacagt
      181 accaggagct tcatttttaa gataagtaaa aagaggatct gtattttctc catttacgtc
      241 aatcaattga tgcataggga aggtaacgcc ataagtggtt ttacaagctt ctgctgcttc
      301 agtcgctgaa tctagctctt gtttaaattg attggaagga aagcctaaga taaccagacc
      361 ttgtgtctga taagcttggt aaagttcttc taattgttta aattgaggtg ctagaccgca
      421 ttttgttgca gtgttaacaa tgattatcgg tttgcctgca tattttttca atgaatagac
      481 agtgccgtct tctaaagtag tttcaaaatt ataaatagac ataaagtttt ctcctttcta
      541 ttttatataa taaaaagagc gctaaactga aaactagcta tttgttttcc cgtttagaac
      601 tcttttcaga caataaatat ttaataaaat acgaagataa gatagctata ttgcttctca
      661 tctttacgtt cttttagaaa ctatgcttct acttcagacc attcatcgcg ttttgctaaa
      721 aattcacgag ccaaagcttg tgcaccttct agtgtatggc tggcagccca gccacattgt
      781 gtttcattgc ttgcaggtac ttcagtagct tcaagaacat ctttcattgt tgcttcaaga
      841 acatctaaaa tgtcttcgta gctttcatga ttcaatacag tcaagtaaaa gcctgtttgg
      901 catcccattg gagaccagtc tacgatgtaa tctgcgtgat tgcggattag ttcggcagtc
      961 aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt ctttatttgg ttgtttaaag
     1021 cggacgtcgt atttaatgat gacatcgcca tgcgcacccg tttttcgatc agctacacgt
     1081 acataaggtg ctttgacttt tgtatggtca agattaaaac tttcgacatt cattctcatt
     1141 tgtaagactc ctatctgttt gttcattcgt tttgatgctt ttcactaaag aattatttag
     1201 tgaaatttac aaaaacgtct ttctacttga ttctaacact tatcgtttcc aaggtgtagc
     1261 aatctgaaat attttttaga tttttctgct aagtcgttta ttgggacttc tagttcttga
     1321 ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg accatactca attcctacag
     1381 aaagacggat caagccgtcc tttatgccag cctcttctcg tttttctttt ggaatagacg
     1441 catgagtcat gacagaagga atttccacta aactttctac gccacctaaa ctttctgcta
     1501 aagtaaatag ttggagtgat tcaacaaatg gaatagcaga ttcttcattt tttaaagtaa
     1561 aagaaatcat tccgctaaag ccgctcatct gctgcttagc aactccatgg agttcatgat
     1621 cgggtaatcc tggatagtag accttttcta ctgctggatg agaaaataga aaatcagcta
     1681 ccacaaaagc atttttttga tgttcctcca tacgaacact tagtgtcttg attccgcgct
     1741 gaagtaacca gctatcttgg ggaccaagta cggctccgat agcattttgg taaaatccga
     1801 tctgatcggc tagatcttta tggttagaag tcactaaacc agctactaca tcgctgtgtc
     1861 cgcctagata tttggtaccg ctatggacaa cgatatcaga accgagatct aaaggtcttt
     1921 gaaaataagg agtagcaaat gtattgtcag caataactat caatccatgt tgatgagcca
     1981 gagtggcact tttttctaaa tcagtaatct ttagaagcgg attgctaggt gtttcaagat
     2041 aaagtgcttt tgtattgggt ttgatggatt gttctatttt atcaagatta ctcgtatcta
     2101 tgatcgtgta ctcgagacca tttttagtaa gaactttgtc aaatagacgg aatgttcctc
     2161 catagacatc atctcctaat aagatatgat cgccagcttg aaaaagagaa aaaacggcat
     2221 gaatcccaga aagaccagaa gaaaaagcga atcctcgaac accgccttcc aaatcagcaa
     2281 tcagttcttc caatgcaaaa cgagtaggat taccagatct tgaatattca tattgtttag
     2341 gctggccgac gccatcttgc cgataagtgg atgtttgata aataggtaca ctgacagctc
     2401 ctgttgtggg atcttcactg atgcctccgt ggatcaattt tgtttgaatg tgcatgtttc
     2461 tttcctccta taagtaaatg ttttttgata aatagcggtc tgctgcgtct ggaaaaatcg
     2521 taacgacacg atgaccaggt ggtaaacgac gaatttctct taatgctgct gcaaaagccg
     2581 caccactcga acttccaact aatagtcctt gttctcgtgc taactgccta gtatagttaa
     2641 acccttctac atcagagatt gtctcgatct gattgattga aaggggagaa agaaacggcg
     2701 gaatgaattc tacaccgata ccctcaattt cgtgaggggc tggatcacca ccatttaaca
     2761 cagagccctc tggttctaca ccgattattc gaatgtcggg atatttttct ttcaaaaagg
     2821 tagaagttcc agcaaaggtt ccaccactcc caattcctgc tacaaaacta tggatatttt
     2881 ctttgatttc atgaaagatt tctggcccta acgtatgata gtaagcagca ggattatctt
     2941 tattttcaaa ttgaagaggc agatagctgt ttgatatctc ctctgcaagt tttttacttt
     3001 tgctgatggc accgatgatt ccttgctcac taggagaatg gatgatcttt gctcccaatg
     3061 ctttcatcaa ttgttgtttt tctaagctaa acttctctgg aacaacaaaa accgttctta
     3121 atttgtatgt gagtgcggcc agagcgatcc caattcctgt atttccggca gttggttcga
     3181 tgatcgtcgt tttttcattg atttttcctt gggcaaatcc ttctttgatc aaataactgc
     3241 ccaatctatc tttgatactt cctccaggat ttaaatattc tagttttgca taaatagcag
     3301 aacctttagg catagaatag tgctgattgt caaagcgata aagcggtgtg gaaccaatag
     3361 cttctgtaat tgtcgagata atcatgtggg cctcctaaaa aaagataata aaaaaaggag
     3421 ctgactatta aatcagctcc ttgaaagaca tagagaaaaa ttgagtagat aaaaagataa
     3481 gttacctggt actcattcta tgaagggcaa ttaatagaca tgacaaatag tatgctgctt
     3541 ttatgcacca tacaacagca acgattcaat tgtttttggt tgctgactgt catgtgtatc
     3601 cctccaagta aaatttttat taacgatagc atgaaaaaaa ataaaagtca aaggcttgaa
     3661 agaaaaaaat atttcgatag catcgtttac ccgtgtttat ttttctgaca aataggcatg
     3721 agaccttaaa agatagagaa tactctagtt ttctggccgg ttgtctgata caataaagtt
     3781 gattatgata aagaaaagaa ggagcaaaaa taatgatctc agcaagtgat ttaagagccg
     3841 gtatgacttt cgtacaagac ggcaaactga ttaaagttct ggatgcaagc caccataaac
     3901 ctggtaaagg aaatacagtt atgcgtatga aactgaaaga tgtccgttca ggtgcaacat
     3961 acgacacaac gttcagacca gaagaaaaat ttgaaaaagc acatatcgac acaaaacctg
     4021 tacaatattt gtacacgatg gatgatacag ctttcttcat ggatttggaa acatatgagc
     4081 aatatgaaat tcctgtagaa acagtagaag aagaaatgaa atatatccta gaaaacatgg
     4141 aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt tcaattacca acaactgtcg
     4201 tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc aacagtcact ggttcaggta
     4261 agccagcgac aatggaaact ggcttagtag tcaatgttcc tgacttcatc gaaacagatg
     4321 aattgttgga aatcaataca gcagaaggtt cttatgtaaa acgtgcgtca aaataattcg
     4381 ctaacggatt gataagaatt tgaaaacaga gcttgacctc ttgaaaggca gatcatcgga
     4441 tctgcctttc aagaggtttt ttgtggaatc agcaaattga aaaataaaaa agtatttgat
     4501 tttatttata ttttgaaaga tgaatggcaa aagctagaga aagaaaaaac tgcacttatt
     4561 tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc gaactattcc gaactatatt
     4621 gaagcgaaaa tattttttca tcactaaata gtgttgaatt tatgttttta tgtataaata
     4681 gcttctaata gttcgggtat tttgttttta taaccatttt tattcttatt tttaaaaaaa
     4741 tatttcgaaa tatactagac aattcagaaa attttgttta atattattcc aataaaattc
     4801 aaatctttat caaatgaaat tccaataaaa acggaaaaaa agtgaaaaga ggaatgaaaa
     4861 aatgacagaa ttgataattg gcggagtcac agcaagcaca ttggcagaaa agtataagac
     4921 acctctgtat gtgtatgatg aagaaaagat gagacaaacg atgctgtcat tcaagcgagg
     4981 ctttgtatcg aaacgttttg atacaaaagt gctttacgcc tctaaagcat tccaaacgat
     5041 tgagatgctg catttagttc gtgaatatgg attcgggcta gatgttgtca gtggtgggga
     5101 aatctatacc gctttgcaag ctgattttcc tagagatact atctacttcc atggtaacaa
     5161 caagacgcca gaggaactga tctatgcact tgaaaatgat ttgttgcata ttgttgccga
     5221 taatttgatg gaagttgaat tattagcaga attaagccaa cgctatttaa aaaaaatctg
     5281 tatcatgctt cggttgaatg tcggagtaga agcacatact catgaatata tcgtcactgc
     5341 gcatattgat tcaaaattcg gcatgagcta tgaaagtaaa gagtgccaac aagcccttca
     5401 gttgataaaa gaaagcagct atctagagtt agaaggattt catgctcata tcgggtcaca
     5461 aatttttgat gttactgctt ggttagctga aatcgacaaa ttgacgaatt acttaaccga
     5521 ttttgaccaa cctctttcct tgaatcttgg cggagggttt gggattcgtt atacacaaaa
     5581 tgatcagccg atcccagtcg aagaggcact aaaaaaactg attgaatata cggaagaagc
     5641 tttggaaaaa cgaaaactga ccattcgtca attactgatc gaaccgggta gaagtattgt
     5701 tggagaagct ggtacaacac tatacacgat tggtttcatc aaaaagacac cacataaaaa
     5761 atattacttt gtagatggcg ggatgacaga taatatccgt ccagctctgt atcaggcaga
     5821 atacgattgt gatttagcag caaaactgtc ggaagaaaaa acagagaaag tgacgattgc
     5881 aggaaaaatg tgtgaatcag gcgatgtggt aattaaagaa gcttatttgc cgaaagcaga
     5941 atcgggtgat cttttagctg tttattccac gggagcttat ggatactcga tgagcagcaa
     6001 ctacaatcga gcgacacgac cagcagttgt ttttgtcaat aatggccaat caagactgat
     6061 tgttcgcaga caagcatttt ctgatctact aaggggggaa gtttcctatg atccttcata
     6121 aatataatgg ctgcggaaat gattttatct tattagatta cgcagaagac acagattata
     6181 gtcaattagc cgcttcttta tgcacaaaag aaaaatatga tatagacgga ttgatcgccg
     6241 taaaaatcga tccgttggaa atgatctatt acaacaaaga tggcagccgc gcgccgatgt
     6301 gcggaaatgg cattcgctgt tttgctcgat acgtaaatga tcagaacatg atgaatgaac
     6361 aggaattcaa cgtagagaca ttagcaggca caagaaaaat tacggtgttg gatccctcac
     6421 ctttttattg cagcgtagat atgggagtgc cagatgattc tccggaaaaa atcaaagcta
     6481 atcaaactta tccaataaaa aaccaggaaa tagagatcaa tggtgaaaaa gtaacgatca
     6541 ctagtttatt tttaggaacg attcataccg ttgtttttgt tgaaaatgcg cgtgaggaag
     6601 tctggagaaa aagaggtgaa ctgatctgtc atcatccttt gttcaaagaa aaaaccaatg
     6661 tcaactttgt agaagtagtc aatgaacaag aattgatcgt ccgtacctat gaaagaggag
     6721 taggttggac acttgcttgc ggtacaggtt gctgtgcctc ttatgtggtt gctaaggaaa
     6781 ctgggaaaat ttcagcagat caggtagttg ttcacttgga acaaggagac ttgatgatct
     6841 ctggtgagga tgcgatacaa atggctggcc cagcagttca tgaatggaca agaatattgg
     6901 aggaatgaag atgataaaag gttcgatcgt ggcgctgatc acgccgatga atgaagaggg
     6961 atcagtagat tatgctggtt tggaaaaatt gatccaattc catttagatg aacaaacaga
     7021 tggattatta gttttaggta caacaggaga atcttctacg ctcacacaat ctgaggagga
     7081 gcagatcctt caactgacag taaagaaagt ggctggccgt gtgccggtga ttgcaggagc
     7141 tggaacgaat aatacgaaag aaacgattga aaaagcgaag cattttgctt ctttgggggc
     7201 agacgcactt ttggtcatca ccccttacta caacaaaaca agtgatgcag gactagctgc
     7261 tcattttaca gcaattgcag aagcctcacc gattccgctt attttgtata atgttccttc
     7321 tagaacaggc atgtcgattc caatccatgt attagtaaat ttagcagaac atcctaatat
     7381 cattggtttg aaagaagcct caggagatat ggcttatgtg atggatgccg ctcggttgat
     7441 tggagaagaa ttcttccttt acagtggaaa tgatgatttg atcctaccag taatgagtgt
     7501 aggaggaagc ggtgtaataa gtgtatgggc gaatatccaa cctaaaatag tccatgaatt
     7561 agtaaaagat acgcaggacg gaagatggca gcaagcaaaa gaaaagcaat taaatgcact
     7621 tgaactgatc catgccttgt tcagtgagac caatcctatt cctgtcaaag cagccatgtc
     7681 attattagac ttaccttccg gacctctacg cctgccttta gtttctcttt caaaagagaa
     7741 aaagaaacaa ttagctcagc ttttgttaaa agaaaggacg gttgaaaaat gaaaatcgga
     7801 ctgattggca gtggacagat gggaaataga gtcgaagaag ttgcacaaga aagaggagat
     7861 acggtccttc ttttacggac ggcacctaaa gaagcaacta cgcttgcttt tacggaactg
     7921 ccagaactgc ttattgattt ttctcatcca gataatttag agaaaatcct ttcgtatagc
     7981 ttatcatatc aacttccgtt agtcattggc acgacaggtt atacacaatc acagttccaa
     8041 gaaataaaag aacatgccaa acatgtacct gtgatgtata gtgctaattt ttcgttcggc
     8101 atcatggtga tgaatcaact aatcaagcaa gcagctgcaa tgttgcaagg ctggcagatc
     8161 gaactgattg aaaaacatca tagtcgtaaa aaagatgcac catctggtac tgcaaatatg
     8221 ctgcttcaaa caatccaaga agtgcaaaag ctgcagcccg tatacaactg gcaagggaaa
     8281 aaaagagaag aaaaccaaat tggtgtacac agtattcgtg caggttcttt acctggtgaa
     8341 catgatgtat tgtttgctgc tactgatgaa atatttacga tcaaacatga atctttttct
     8401 aatcgaatct ttgctgcagg cgcagtagaa gcagcggact ggttgaaaga tcagtcacca
     8461 ggttattaca aattagaaga tttattaatg gacaaagggg tgtaaaaaac gtggaagctc
     8521 aagaaatcat ccgctacatt caaacggcat ctaaaaagac gcctgtgaaa gtctatctga
     8581 atgtaaccga gccaatcgaa tttaaagaaa gtaaagtttt tggtgaagga agcagcttcg
     8641 ttgtttttgg tgactatgag acgattgctc cggttttaga agctgaatca gaaaaaatca
     8701 ttgaaatcga aatcgaaact gctgcaagat acagcgcagt tcctttgcta gatattaaaa
     8761 aaattaatgc tcgtatcgaa ccaggtgcta ttatcagaga tcaagtgtca atcggcaaca
     8821 atgccgtgat catgatggga gcgattatca atattggtgc agtaatcggt gaaaacacga
     8881 tgattgatat gggtgctgtc ttaggtggac gagcgacagt gggcaaaaac tgccatattg
     8941 gtgcaggagc tgtcttagca ggtgtgatcg aaccagcatc ggccaaaccg gttatcgtag
     9001 aagacggcgt actggtaggt gcgaatgcag tgattgttga aggtgtccat atcggaaaag
     9061 atgcagtcgt ggcagcagga gcagtggttt tagaagatgt ggcagcagaa actgttgtcg
     9121 gtggaatacc tgcacgtgta ttaaaaataa gtgatgatca gacaaaagaa aatacagcat
     9181 tgattgctgc gttacgcgaa ttatagagga gatggagaaa aatgcgcgaa atagatacct
     9241 gtattgtggg ggcaaccgga ttagtcggcc aaacgatgct caaagtgtta gaagaatatg
     9301 attttccagt aggacgattg aaattattag cttcaaaacg ttcagccggt aaagtttgtg
     9361 tattcaagaa aaagaaacac actgtacagg aattgacaga aacttcattt gaaggatatg
     9421 atttagcgct attttctgca ggtgcttctg tatccaaaga gtttgcgcca atcgcacgaa
     9481 gtcagggact atttgtcata gataattcct cagcatggcg ggaagattcg gatattccat
     9541 taatcgttcc agaggtcaat ctggaagact atacattgaa tcggctgatt gcaaatccaa
     9601 attgttcaac cattcaagcg gtacttccgc ttaaagcttt acaagaagcc tttggtatcc
     9661 agcgagtcaa ttattcgacc tatcaagcag tttctggttc cgggcaaaaa ggcattgaag
     9721 atcttcatcg tacgcaaaat gacgaggaac cagttttgta tccttataat attagccaaa
     9781 cggtgattcc ggaaatcgat cagcctttgg aaaatgggta tacaaaagaa gaacagaaaa
     9841 tgataaatga gacccgtaag attctgcatc agcctgcttt gccagtatca gcaacctgcg
     9901 tacgtgtgcc aatcgaaaat ggacatggag tctctgtggc agttcaactg caaaaagagt
     9961 ttacgacgga agaaattaga gagtgtctca gccagtttcc agggattcaa ttagaagatg
    10021 atctagcagc acataaatat ccgacgagca ttcttgctag aggaacagat atggtttatg
    10081 ttgggaggat cagaaaagac acttctttag agaacggatt gttgttctat acgactgccg
    10141 ataacatacg caaaggagcc gctgcgaatg ccgtacagat tgctgcagct ttaatgcaga
    10201 aaatagcgga ggtggcaaga tgagtaatac gctgaaagta gcgaaattcg gcggaacatc
    10261 gatgtctcat gaagagcaat tcaaaaaagt ccgtacaatt gttctttcag atcccaatcg
    10321 ccaagtgatc gttgtttctg ctttaggcaa acgtacgtca gatgatgaca aagtaacaga
    10381 cttactttat ttgatttatg cttaccttca gcatcatgtt gattggacac ccctttggaa
    10441 aaggatttgt gatcgtttca ttcaagtaag agatgttctt gccctgcagt tacctattga
    10501 agatgaacta gcacgcatac agaaacaact ggaagacgaa aagataactg aagattattt
    10561 agtaagtaga ggtgaatatc tgactgctca actgatggct gagtatcttg gttaccagtt
    10621 tattgacgca aaagacctga ttttctttga ctatgatggg aaaatcgatc aggaaaaaac
    10681 agcacaggaa atcacaaaag catttaaaag atatccgaaa atggttgttc cagggtttta
    10741 cggggcgaac cctgcaggaa aagaaaaatt attgggccgt gggggaagcg acattaccgg
    10801 cgcgatactg gcgaagatgt tgcaagcgga aagttatgag aattggacgg atgtttcagg
    10861 aatcatgatg gctgatccgc gaatcatcga tcatcctaaa atcatcgagg aaatctcttt
    10921 tagagaactg agagagatgg cttatatggg ggctaacgtt cttcatgaag cagctgtttt
    10981 cccagttcaa gaggctgata tccccatcca aataaaaaat accaatgatc ctttcgctca
    11041 agggacaaaa atatcgaatc acgaaataga aaaggaaaat ggactgacag gaatagctgg
    11101 acgcaaggat tttctttcca tcactttatt caaacgtcat atgtccgatg aaatcgggtt
    11161 tatctggaaa gcaatgagca tttttgctaa acatgagata tcaatcgaac atattccttc
    11221 agggatcgac aatatcggag tgatcgtctc agcagaagcg attgccgata agttatttct
    11281 catcacgaaa gaattaaaag aaacacttgg cgtggaagaa atcgtcgtga tcgaagactt
    11341 agctttgatc tctgtcgttg ggggtccgca taaagaattg attggattat ctgggaaagt
    11401 gttatcgata ttgaacaaat tagaaattcg cacatcgatc ttatcacaag gagcacagga
    11461 gttgaatctg atcatcggtg taccgaataa tcaatatgaa acagtagtga agggtatcta
    11521 tgaagggatg gtgaatacgg atgctgaaag aatatcgaat ggcactccat caaattcctg
    11581 aaatcgggtt tcatgaattc aagacaaaaa aatatttata tgaacaagta aaaaacagcg
    11641 aggccgttat ccatgaaatc ggcgaaacag gactactcct ttattttgat aatcagcaag
    11701 aagaaacgat tgcttttcga acagatatcg atgcactgcc agtcaaagaa gaaacgaatc
    11761 tctcttttgc ttctaagcat gaaggttata tgcatgcatg tggtcatgac ggacatatgg
    11821 cgatgctttt agggttgact gactttataa ctaaacacat tgaggatctt atttataatg
    11881 tagtactgat ctatcagcca tcagaagaaa tttccggtgg cgcacaaagc gttattcagt
    11941 caggattgtt ggataaatat gaagtacaag caatatttgg ctttcatcta tggcctggac
    12001 tagctaaggg agaagttttc tctcgaccag gacctttgat ggcacaaagc agtgaaacag
    12061 acattatcat taaaggccag tctgcacata ttgcttctag tgaaaaagga atcgatagtc
    12121 tggaggcagc tgtacgattt ttgaagaatg tttatgattt tgaccggcaa ttaccggcag
    12181 agtggaatca tctaatgaaa tttggccaaa tccaaggtgg aacgatccga aatgttttag
    12241 ctggagaagt tattgtatcc ggaagtatcc gttcttacag ttcagatatg caaaattatt
    12301 tgaagagggg attagaccat ttagcagaac agtttcactc tacagctact atttcttttc
    12361 gctataatga cggctatcca gctgtaatca accatagagg cttatatgca gcactgttag
    12421 attcaaacaa actacatgaa ttagatgctc cagttcttca agccgaagat tttggcatat
    12481 atacgcaaca atatccatgt gtatttttct ttttaggtgt cggtgatacc cctgctttac
    12541 atgatccgac gttcgatttt gatatggatg ttttagaaaa tggtgtagaa tggtacaaaa
    12601 cgattctttt tacagaaaat cttcttgtaa aaatcgatag cagttcaaaa gaggagcgag
    12661 taaataatga tgaatttatc caatcgattt agtcctaggc tttctagaat agaagtatca
    12721 aaaatacgac aattcgatca gcaaatatcg tcgataccag atgtgatcaa actcaccctg
    12781 ggagaaccgg attttccaac tcctgaacat gtcaagcaag caggaatagc agcgattgaa
    12841 gaagactttt ctcactatac cggaatgaga ggattagagg aactgcggga agcggcttgt
    12901 atatttcagc aacagcgtta tggattaact tatgatccgc aaacggaagt tttgacgaca
    12961 gttggtgcaa cggaagcgat tgcctctgcg ttgttatctg ttttggaaga aggagacaag
    13021 gttcttattc cagcgcctgc ttattcagga tatcagccat tagtggaact agctggtgct
    13081 gaattgatcc cgatagatac ttctgacaca gggtttgtct gtcagccgga acaatttgaa
    13141 cgagcatttg aacaatatgg atcagctgtc aaagcagtca ttttaaacta tcctaacaat
    13201 cctactggga cgacattgtc tgcaaaacaa ttgaaagcaa tcgctgaggt tttaaaaaaa
    13261 tatccggtat ttgttataag tgatgaagta tatgctgaat tgacttatag tggaacgcat
    13321 atgtccatag ctacttatct tccagaacaa acaatcgtga taagcgggtt atccaagtca
    13381 catgcgatga ctggttggcg agtgggcttt atttttgctc aaaaagccct tattgacgaa
    13441 ctaattaaag tccatcaata tctggtgact gccgctacaa cgatgtcgca aaaagctgcg
    13501 gctgaagcgc taataaatgg ggtgaaagat agcgagaata tgaaagagca atatcaaaca
    13561 cgaagagact atctggtcca gcaattggcg cctttaggat ttgaagtgac ccagccaaac
    13621 ggtgcatttt acttgttttg caaactccca gctgccattc aaactgactc ttggcagttt
    13681 tgtgtagaat tagcggaaca ggcaaaagtt gcgtgtatcc caggaattgc atttggccca
    13741 gaaggagaag gttatatccg gatttcttat gctagcagca tggaaaaatt acatgaggcc
    13801 tgcaaacgga tcaaagactt tttaacagaa cagtgattca tagattttat caaaaaaaca
    13861 atcgttaatt ttgcggtata ttctgcaagg agacgattgt tttttaaatt tagttcaaat
    13921 gaacgcgttt cttttgattc ataactaatg taatgttaag tttaaattgt ttagaatgat
    13981 tttaaaagaa tattcaagga ggtttcatca atggaaaaaa tcaaagttgt cattgtaggt
    14041 gcatctcatg gagggcatca atcgatcttg gaactattga cacgatacgg tgaaaatgta
    14101 gatatcacgc tatttgaagc aggagactat atttccttca tgtcatgtgg tatggagctg
    14161 tatttagaag acaaggtaac agatgtaaat gatgtacgta atttcagacc agaaaacttt
    14221 ccgcaatcca atgttcatat tttaaataat cacgaagtaa aaacgatcga tgccgataaa
    14281 aaaacagtaa cggttactag cgcatcagat aacgaaacaa aagaatatcc ttatgacaaa
    14341 ttgattttga gttctggtgt caaaccaaac aatctgccag taccaggagc agatctagaa
    14401 aatgtctact tgatgcgtgg atacaattgg gcaaccaaaa tcaaagaaaa attaaatgat
    14461 ccggctatta aaaaaatcgc tgtcgtaggt gcgggttaca ttgggatcga agcagcagaa
    14521 gcaagccgaa aagctgggaa agaagtcgta ttgcttgatg tgatcgatag acctttaggc
    14581 acttatttag atgctgagat gacagatatt ttagaacaac acctaaatga aaacggtatt
    14641 gaagttttga caaatgcaaa aatcgaagct tttactggta acggaaaagt tgaggcaatc
    14701 aaaacaagtg aaaaagaaat cccagcagat ctagtcattc aagctgccgg tgtgaaggca
    14761 aatacagaat ggttaaaagg gatcgtcgac ttagatgaac gtggctggat ccaaacaaat
    14821 gaatacttgc aaacgaatct tccagatgtg tatgcagtag gagatgctac acttgcttat
    14881 tctatcccag cacgcaaaaa aatgccgatc gctttagcga ctgttgcacg tagagaagca
    14941 cgttatgtag taaaacatct ttttgaagaa gtaccaaaca aaccgttcgg cggagtcgta
    15001 ggttcctctg cattaagtgt ctttgactat cattttgcag caagcggatt gaatagcttt
    15061 acagcagcaa aagcaggagt agatgtacga gtgtcttatt atgaagatac gttacgacca
    15121 aaatatgtac cagaagaaca aggaaatcca aaagtgtcca tccaattatt ctcggatcca
    15181 atgacccatc aattgctagg aggagcagta ttatctacct atgatgttac aggtcaagga
    15241 aatgtattgg cactagcgat ccaaaacaaa ctgactttag aagaactagc agaagctgac
    15301 ttcttcttcc agccaggctt tgaccgacaa tggagcatgt tgaatctagc cgctcaacaa
    15361 gcgttaggag aagaaccatt tgtagaataa gaaaacgaaa tatttgaaaa gaggttgtga
    15421 aaaagctgcc ttgcatcaag taataagaac agccaagtaa aaacagcttc ttatgttttt
    15481 aacttggctg gacttattac cgcagatgca agcttttgaa caccgtttat tttcaaaaaa
    15541 gggactgtga cacgatttgt gtaccgaccc ccaaaagtta gaccaaaaaa tctaactttt
    15601 ggaggtcggt attttttatg gttaaatata cttttgaact taaattaaag atcgttcatg
    15661 attatttgga tggcaaaggc gattggaccg taaaaatcca gatcaagaga ttgaagcgga
    15721 aatgcttaaa attcgggaga agcacaagga ttatggctgt ttacggatga ccaatgaact
    15781 aagaaaccgc ggtttctcta tcaataaaaa gaaagttcag cgcttgatca agaagctagg
    15841 aataaaagta acggcataca cgcgtaaatc acgtcgctac aattcttatc gaggaaaagt
//
LOCUS       DAZBTF010000062        15641 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000062 DAZBTF010000000
VERSION     DAZBTF010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.062"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            240..887
                     /locus_tag="ACHGNS_002116"
     CDS             240..887
                     /locus_tag="ACHGNS_002116"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFN6938042.1"
                     /translation="MPNKNYRVYKRGTIVYVDFGVNVGYELSGNHFAIVLNNKDNSKN
                     GVVCVVPISSKKKSNYVSVGKILEVASIKQFISQADKLKEKLRIIILLSLNRGIIDES
                     DLNSFIKDIEAKGEKLSVEEVEKRAVKHNLVCETREQVIKEIKSLSEDMVKYQKVFNC
                     YKKYTQESYVMPLNIQTISKNRIRRINKFDPSGNMKAPGNVLNEVDESLKKYFTK"
     gene            complement(1212..1358)
                     /locus_tag="ACHGNS_002117"
                     /pseudo
     CDS             complement(1212..1358)
                     /locus_tag="ACHGNS_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            1597..2289
                     /locus_tag="ACHGNS_002118"
     CDS             1597..2289
                     /locus_tag="ACHGNS_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFN6938043.1"
                     /translation="MNKKIVVSGLIIGGLVLAGIAGKNFADDILLWGGEDNIEQINHN
                     LESLDKALENKEQNISSLNSRLSSNNQELEQAKANVEEYKQKVLTLENDKNQLTIEKT
                     NLENQLSGKNAELQGKQNEINAKIDEINQKIAEINQLNTDWNNRLQTEQNKFNDAQNT
                     VNSLNQQINQLSNEKNTIKNQLQSTEQKLEKAQSENSNLNNYIEKLEKAKSDVEDTAN
                     RSQEIVTEHTGK"
     gene            complement(2571..2643)
                     /locus_tag="ACHGNS_002119"
     tRNA            complement(2571..2643)
                     /locus_tag="ACHGNS_002119"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2608..2610),aa:Lys,seq:ctt)
     gene            3024..3593
                     /locus_tag="ACHGNS_002120"
     CDS             3024..3593
                     /locus_tag="ACHGNS_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFN6938044.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            3580..4440
                     /locus_tag="ACHGNS_002121"
     CDS             3580..4440
                     /locus_tag="ACHGNS_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFN6938045.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            4630..5334
                     /gene="yycF"
                     /locus_tag="ACHGNS_002122"
     CDS             4630..5334
                     /gene="yycF"
                     /locus_tag="ACHGNS_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFN6938046.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            5339..7174
                     /gene="walK"
                     /locus_tag="ACHGNS_002123"
     CDS             5339..7174
                     /gene="walK"
                     /locus_tag="ACHGNS_002123"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFN6938047.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            7171..8487
                     /gene="yycH"
                     /locus_tag="ACHGNS_002124"
     CDS             7171..8487
                     /gene="yycH"
                     /locus_tag="ACHGNS_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFN6938048.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            8488..9357
                     /locus_tag="ACHGNS_002125"
     CDS             8488..9357
                     /locus_tag="ACHGNS_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFN6938049.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            9412..10221
                     /locus_tag="ACHGNS_002126"
     CDS             9412..10221
                     /locus_tag="ACHGNS_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFN6938050.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(10324..10953)
                     /locus_tag="ACHGNS_002127"
     CDS             complement(10324..10953)
                     /locus_tag="ACHGNS_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFN6938051.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            11123..12415
                     /locus_tag="ACHGNS_002128"
     CDS             11123..12415
                     /locus_tag="ACHGNS_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN6938052.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(12491..13153)
                     /locus_tag="ACHGNS_002129"
     CDS             complement(12491..13153)
                     /locus_tag="ACHGNS_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFN6938053.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            13378..13662
                     /gene="groES"
                     /locus_tag="ACHGNS_002130"
     CDS             13378..13662
                     /gene="groES"
                     /locus_tag="ACHGNS_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFN6938054.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            13713..15338
                     /gene="groL"
                     /locus_tag="ACHGNS_002131"
     CDS             13713..15338
                     /gene="groL"
                     /locus_tag="ACHGNS_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFN6938055.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         5352 a         2572 c         3274 g         4443 t
ORIGIN      
        1 tgaggggaag tacataatgc aaatttgtcc ataacttatt gattatcttg taaaaatgcg
       61 gtaatttaat agttagtgag aagaggtgct taattatagt taaaaaagag gaaatagaag
      121 cagcatgtaa ccaagcaaat gttaggttta ttggagaagc tttatctgga tatcctaagt
      181 ataaatactt acacaactgg ttacaaagta aaagttttta tcttaataaa gaaagatgca
      241 tgcctaataa aaattatagg gtttataaaa gaggaactat tgtatatgtt gattttgggg
      301 tgaacgtggg ttatgagtta tctggtaatc attttgctat tgttttaaat aataaagata
      361 attcgaaaaa tggtgttgta tgtgtagtac caatctcttc aaaaaagaaa agtaattatg
      421 tatctgtagg aaaaatatta gaagttgcta gtattaaaca atttataagc caagctgata
      481 aattaaaaga gaaacttagg ataattattt tactttcatt aaatagaggc atcattgatg
      541 aaagcgattt gaatagtttt attaaagata ttgaagctaa aggtgaaaag ctttcagtgg
      601 aagaagtaga aaaacgagct gtaaaacata atttggtttg cgagacaagg gaacaagtaa
      661 ttaaagaaat aaaatcatta tctgaagata tggtaaaata tcagaaggtg tttaattgct
      721 acaagaaata tacacaagaa tcctatgtaa tgccacttaa tatccaaacg attagtaaaa
      781 atagaattag aagaattaac aagtttgatc cttctggaaa tatgaaggca cctggaaatg
      841 tcctcaatga agtagatgaa agtttaaaaa aatattttac aaagtaaacg atatttttgt
      901 tgacattttt ctgttcgtta gttaccatgt agttatagaa ggacgtaagc gcctattttt
      961 aatgtacaca tcgggacgta tgcgcccaca tagaaacata gttgagagac atctatatgg
     1021 tgtctctttt tttattaata tgcaaaaaag tctagttttt tcaataatat ttatgtaata
     1081 ataaattagc caccacaaca aagaatgaaa tccattatta tctagtctag tccattcttt
     1141 ttgtttgcag tatttgtgat ggctttccgt acccttagct cagttggtca gagcagacgg
     1201 ctcataaccg tccggtcgta cttctcgtaa tccaggaagg ccgtcagctc cgtccgcagt
     1261 agcgtgttta cggccagctc cagatgggag cggaataact cgttcaaatc acctttggtg
     1321 gctagagtct gcaggagttc tgtaataaaa tcgttcatag ggaagtcctc ttttctgtga
     1381 atgggtgtgg taactttatt ctacagaagg gacttccttt tttctattta cacaaaatat
     1441 tttatactct caaatgtttg tatgcagtta catctgactt tattcgaatt aaggaaaaga
     1501 tttataaaaa taaaataaaa aaatatatat ttaaattgac ttattgtgaa atcattaact
     1561 ataattagtt cagtttatgt taaggagaat gatgatatga ataagaaaat tgtagtaagt
     1621 ggattaatta taggtggatt agtgttagca gggatcgcag ggaaaaactt tgccgatgac
     1681 attttgttat ggggtggaga agataatatt gaacaaatca atcataattt ggagtcatta
     1741 gataaagcac tagaaaataa agagcaaaat attagtagtt taaattcacg attatcttca
     1801 aataatcaag aacttgagca agcaaaggct aacgttgaag aatataaaca aaaagtgttg
     1861 actttggaaa atgataaaaa tcaattaact atagagaaaa caaatttaga gaatcaatta
     1921 tcaggaaaaa atgctgaatt gcaaggtaaa caaaatgaaa tcaatgctaa aattgatgaa
     1981 ataaatcaaa aaatagctga aataaatcaa ttaaacactg attggaataa tcgtttacaa
     2041 acagaacaaa ataaatttaa tgatgctcaa aatactgtta atagtttaaa tcaacaaatt
     2101 aatcaattgt caaatgaaaa aaatactatt aaaaaccaat tgcaaagtac tgaacaaaaa
     2161 ctagaaaaag cgcaatcaga aaacagtaat ttgaataatt atattgaaaa actggaaaaa
     2221 gccaaaagcg atgtagaaga cacagcaaac agatcacaag aaatcgtgac tgaacataca
     2281 ggaaaataga tgcatttttt agtttaaatt tctttaacaa aatagtattt tgttgctaaa
     2341 taatttttat ttgaatattc tacaatgata tctcccctca aaatggaggg gaagtacata
     2401 ctgtgataat ctacaggttt attgactgat taggatgtta ttaaaaaata aaatactaaa
     2461 atgcataccc gaatttcata cccgaaaaat aaaaaatgcc accacgtaca ctcttttcga
     2521 aactacctaa gtagccaaga atgtactggt gacgtgggtt tggcacccaa tgggccgtga
     2581 ggggctcgaa cccgcgaccc gctgattaag agtcagctgc tctaccaact gagctaacgg
     2641 cccgtacaaa aagtacttaa taatagtagc actaagaatt ttaaaatgca agactacatt
     2701 taaagaaaat cagaaaaatc tttacttcct tataggaaat cattcataga ttattcacat
     2761 ttgctcgcta ttatataagc ataccaacaa acaaagttct tgcaagagaa caaaccctaa
     2821 cacttttttc attttttact cctttcccgc cgtctccccg gcgggtctcc tgttcttaac
     2881 gataaaagac aagaaggctt agccttcttg ttttttgttt tcatggaatc ccgctaatca
     2941 aatcacctag ttaaagcaac tagattgaat gtttatcata agtctgatat catgtttatg
     3001 aaatgaaaaa aggagcggtt atgatggaaa aaatgcgtca gcaattaatg gagtggggga
     3061 aggaactaga aacattccgt ctgcctcact gggaagaact ccctgactta gaactttata
     3121 tggaccaagt cagaacgtta gttgatcgtt atctttcacc tgtgatacaa ggagaaaagc
     3181 atccattgct tacctcttct atggtaaaca attacgttaa actaggtttg ataccagcac
     3241 cagttaaaaa aagatataac aaagaacacg tagcattttt acttgcaatc actacgttga
     3301 aacaagtatt gactattcct gaaatcaaag aagggatatt gttccaagga aaaacagtag
     3361 gaattcgtga agcctataat ctattttgcg atgaacaaga agcagctgtg tggatggtca
     3421 gccaattagc acaaggtaaa tctcatccgc aaaaattcga ccaggcaacc cctgtagaat
     3481 atattgctgt aagagcggca actttatcgt ttgcaatgaa acttcttgcg gaaaaaacga
     3541 ttgttctaga aaccgaatat ttaaaagagg agaaaacaaa tgaaaaacaa taaaatcgca
     3601 attttagtag attcaggtac ggatgtgcca gaagaattga taaaaaaata cgagatgtat
     3661 atgatcccat tgaaaattat ttataaagat caggtatata cagataaaat tgacattacc
     3721 tctgaagaga tctatcaacg tttgccagag gaaatccctg gtacatcttt gccggatggt
     3781 gagtcgatca cagaaatctt tgagcaaatc aaaaaagacg gttatgaaaa ggtacttgcg
     3841 gtaacaattt ccagtggatt gagcgggaca tataatgtgg ttcgtttaat tgcacaacag
     3901 caagaagaat tggaagcata tgtactagat acgaaaaata ttggtattgg tgcaggtttt
     3961 tctgcgatcc aagcagcaaa atggctggaa gaaggagtag aatgggatca actgatcagt
     4021 aatctgaatg agttagtcga aagaacaaaa gttttcttta atgttgcgac attggaatat
     4081 cttcaaaaag gtggacggat cggtttagtt gcttctattg taggaaccgc tttaaagctt
     4141 aacccaatca tctcatgtaa tgaagaaggg atttattaca cagtaggaaa agctagggga
     4201 cgtaaaaaaa gtttggataa aacggttgct tacgtcaaag aaaaaatcgg taatgcaaag
     4261 gtcttcaact tagctgttgc ccatggggat gcgaaagaag aagcaattga aatggaagcg
     4321 agacttaaaa gagagttccc acaagcaaac caaatctact ttggccagat ttctccggca
     4381 ttagtagtcc acactggtcc gggattattg ggtgtcggtg tacaagtttt agaaggataa
     4441 atcaaaaaaa ggaaacactg aggtgaaaat cagtgttttc cttttttatt gaagttagaa
     4501 caaaaaataa taaagtattt tgcactttca ttttaatttt ctcttttgtt ttataggcaa
     4561 agatgaaaca aaagcagaaa tacggtataa tgagaaggta tgacaataat atataagagg
     4621 tggctcttta tgaaaaaagt tttagttgtc gatgatgaga agccgatttc ggatatcgtc
     4681 aaattcaatt tagccaaaga aggctatgat gtttatactg catatgatgg ggaagaagcc
     4741 ttggaaaaag ttgctgaggt agaacctgat ttgattttac ttgatttgat gctacctaaa
     4801 atggacggct tagaagttgc tcgtgaggta cgcaaaactt atgatatgcc gatcattatg
     4861 gtaacagcca aagattctga aattgataaa gtcctaggct tagagttagg cgccgatgat
     4921 tacgtgacca agccattttc caaccgagaa ttagttgcac gtgtcaaagc aaatcttcgt
     4981 cgcggtgcca cggctgcgaa ggaaccagaa gaggcagcac ctgcggaatt gacgattggt
     5041 gatttgacga tccatccaga agcttacatg gtgacgaaac gaggagaaac aatcgagtta
     5101 acacatcgtg aattcgaact actcttttac ttggcgaagc accttggaca agtgatgaca
     5161 agagaacatt tattacaaac ggtttggggt tatgattatt ttggagatgt acgaacagtg
     5221 gatgtaactg tacgtcgttt gcgtgaaaag atcgaagata atccaagcca tccaaattat
     5281 ttagtgacac gtcgtggtgt cggttactat ttgcgtaacc cagaacagga gtaattggat
     5341 gaaaagaaaa gtccgctttt ttcgatcagt caattttaag atcgctatta cctttattct
     5401 gattttattg atctcgatcg aaattattgg tgcctacttt atacgtggtt tagaacgttc
     5461 cacgatcaat acatttatta aagatatgaa tcaaacggtg gaatcattag caacaacaat
     5521 cagcccggaa ttgaatcgaa aagataatgc agatgatgaa gaggtaaatg cgaatatcaa
     5581 aagatttatt gaaaacagtg cgactagcga tatcatcgaa atacgggtcg ttgacgaaaa
     5641 agggatcatc cgaggaacaa ccgatgtcaa tgaacaaagc gctgtgggaa agaaaaatga
     5701 ttatgttgat atcaatgatt tcacgactaa acgttatgtt gctttagata acgacgataa
     5761 acgggtgaat atcaatgttc aaccgatcct gtccccgacc ggcgatgccg ttatcggggc
     5821 tctttacgtc aaaagtaata tcgaacaaaa atattcggaa atcaataaca cagcagtcat
     5881 tttctttact gcctcactaa ttgcggcatt tatttcaatg atcgtatctg ttttagttgc
     5941 tcgttcgatt acacagccga tcggtgaaat gagagaacaa gccattcgaa tcgctagagg
     6001 agactacagt cggaaagtca aagtgcatgg acaagatgaa cttggacagc tggctgatac
     6061 cttcaatcag ttagctgaac gtattgaaga agctcaagat acaatggaag ctgaacgcaa
     6121 tcgtttggat agcgtgctat cccatatgac agacggcgtc attgcgacag atcgcagggg
     6181 taaagtcatt acgatcaatg atatggctgt ttccttgcta gatatcaaaa acgaggaagc
     6241 aatcggacaa tcgattttga cacttttaga tatcgaagaa gaatatacgt tgcggaaatt
     6301 attagaagat ccaaacgaaa tggtacttga ccgttcaacc agtacattgg aaagtgatca
     6361 gatgatcttg cgagtagact tttctatgat ccgtcgcgaa tcaggtttta tcagcggatt
     6421 agtagctgtc ttgcatgatg tgacagaaca agaaaaaaca gaacgggaac gtcgtgaatt
     6481 cgtctctaat gtttcccatg aacttcgtac accgttgaca agtatgcgta gttatatcga
     6541 agcattgagc gaaggtgcgt ggaaagacga ggaagtagca ccaaattttt tgaaggtaac
     6601 gctagatgaa acagaccgaa tgattcgaat gatcaatgac ctcttaaatc tatctcgcat
     6661 ggatacaggt aatacccagc ttcaattaga atatgtcaat ttcaacgagt tagtgaattt
     6721 tgttcttgac cggtttgata tgatggtggg aaatcaggaa aaaaattata agatccgtcg
     6781 tgaatttacg caaagagatc tttgggtcga agtagataca gataaaatca tccaagtagt
     6841 cgataatatc atgaataacg cgatcaagta ttcaccagat gggggaacga ttacttgtcg
     6901 cttattagaa acgcataata acgtggtttt aagtatcacc gatcaaggat tggggattcc
     6961 taaaaaagat ctgaaccgag tgttcgagcg gttctatcga gtagataaag cacgtgcaag
     7021 agcacaaggt gggacaggac ttggattagc tatttctaga gaagtgatta aggcgcatcg
     7081 aggcgctatt tgggcagaga gcaaggaagg aaaagggtcg actttttata tctcgctgcc
     7141 atatgaacct tatgaggagg aatggtggga atgagaatct ctgaaaaagt cgttcgtata
     7201 ggactcattt tgatggttgc gttaagcata tacttctctt atgcaatctg gctgagccca
     7261 gcaggaaaaa gttcaatcaa cgtagatgag acaaacactc aaatgatcga gtctcaaagc
     7321 aatcgaaaaa cttccgaagt ctttcttccg cttcatgtta catggcacca ttcgggaatg
     7381 atccaagaaa ccaacagtga aaatctcgtg taccgattgc aattgatcat cgataaagca
     7441 agatttggaa agctttctga agtagtttca ggagacaaat cacagtttga acaggtgaaa
     7501 aatctgaact ctggttttga gttggcctat aatgcgcctt ttttgctaag agaatacaag
     7561 gaagcatttg atttagattt agatgtgtcg aacttggcaa aagaaagcga aaatatgtat
     7621 tttactagaa tacaattcga tcaaggaaat aaaaaaatcc gatttttgaa ttattcgaac
     7681 catctcgtat atgaagcaaa tatttcgttg aattggacag atatcaataa agaattaaca
     7741 gcaaaagaca caaaatggac agaaatgaca acagaaccat cgacgatcga cacgcaatat
     7801 gctacgaaac attcgatcaa actgaaaaag tatagttata tcctgtcaca gcaaccctat
     7861 acacttttta gaaatgcttt tttccagaaa ccagatagcg tgaaaaataa tgatgaaagc
     7921 aatgaattga ttttttacgg tgggaatgaa acaatgtcta tccattcgga aacgcaagta
     7981 gttgattttc gtggagagct gccagagaaa aaagaaaatg ctagtgtatt ttctgaaagt
     8041 ttcccctata ttagcaaatt aggcagttct ttagggaacc tcagatattt tgaccgcagt
     8101 gacaatacga tcgattatcg gatattcgtg gaaggcttcc cggtttttgg gacagacagc
     8161 agaggacaga ttggtgtgga agttggcggt gaaacgaatg gttctgtgca agtgaatatc
     8221 caaaccagtt tgaatacgat tcaagtgccg ataccttcag acgaagaagt agaacttgca
     8281 agtacgaatg aaatcatcca gcagttaaaa gctaagggag cagatagcaa caagattggt
     8341 tcagtgatcg ttggctatac atggcaaaat gtcaaagaaa cgaatcgtgt cgttgatctc
     8401 ttacctgaat ggtatgtgaa atataaagat aactggtatt cagctaacga ccttctggaa
     8461 aaattacctg gattggaggc gaattaattg gatttcaaaa aaatcgaatg gatatttttc
     8521 gtcgcttttt tagggttgaa tgtgttttta ttcagcagtt atcatgacgc ggtataccaa
     8581 gaaaacaatg tcattcgctc taatcagact gaatcaatcc aacaacgtct ggctagcgac
     8641 gatatcactt acacggggaa gatttcgtct gagaacaagc aaggctatta tcttagcgcg
     8701 gagcagacca atcttagcac ttcattagcg aattatcgca aggctggtaa aagcggcttg
     8761 ctggcaagtg gtgtgttttt agacggtgat gttttgacga aatctttatc cagttcagat
     8821 gctgaaaaga acgtgatcga tccagataaa attagtagtt ccttggaaga ttttttatct
     8881 aacaaagaca atattttatt cggtaatgat tatgtgtaca cgaaaaactt ttctcattta
     8941 gaagaagaac cattagaaat ccaagcaagc caagagtatg aaggcatccc attcaatgat
     9001 gatacagcaa aaatcgttct ttctttagaa aaaaacgata atgattatag catcacgaaa
     9061 tacatgcaga cgcatttatc tgatatcgag cagctgcgcg aaaagacgga actgtatacg
     9121 gaagaggaag caatcaatac attgtacatc aataacaaaa taccaagagg atcaaaaatt
     9181 ttatggcgtc agctagctta ttcctgtatt ctgaaagtcc gggaaaaaaa tgtgtatgtt
     9241 cctgtttggc atgtagcgat cgaaacaagc gataaagtgg tccaaattga gagtgtcaac
     9301 gctttcagca atacgatcgt gacaaacaat actgtaccaa aagtggaaga tcaataaaaa
     9361 agcgagtata atgaaaaaag tttacaaagt aagaagggaa gtaggagtca aatggctgaa
     9421 gaaaaaccag cttttaaaat cagtatccta gcaagtggaa gttcgggtaa ttcactttat
     9481 atcgaaagtg aaaaaaaacg gcttttggtc gatgcaggac ttagcggtaa aaaaatcact
     9541 tccttgatgg ctcaaattga tcggacaccg gaccaattag atggaatact tgtgacgcat
     9601 gaacatcgag atcatataca tggcgtgggt gtcttggctc gtaagtatca tttggatgtt
     9661 tttgccaatc agcagacttg ggaagcgatg gaaccactga ttggaaatgt accagtggaa
     9721 caaaaacatc tatttgagat ggggaaagtc agaacattcg gcgatatcga tattgagagt
     9781 ttcggcgtct cccatgacgc agcggctccg caattttatc gtttccataa agaaaatcgg
     9841 tcattcgtca tgttgacgga caccggatat tgcagtgatt atatgcgagg tgtcattgaa
     9901 aatgccgatg gttatttgat ggaaagtaat catgatcttg agatgttacg catgggacct
     9961 tatccgtgga atttaaaaca gcgtatttta ggagatagag gacatctttc caacgaagac
    10021 ggtgcacttg tcatgactga tgtgatcggt aatcggacca aacgaatcta tcttggccat
    10081 cttagcaagg aaaacaatat gaaagaactt gctcatttaa caatggaaaa tgttttgaaa
    10141 gaaaaagatt ttggcgttgg acatgatttt gaaatctatg atacagatcc agatgaagca
    10201 actgagctat tttcgattta aacagttcaa tcaataaaaa tataaaaagc agaagataaa
    10261 atcaatcagg gttggtttta tcttctattt ttattttttt agcatattaa aaaaataacc
    10321 gtattatcga ctgactacac tgatgtgctg cttttcatgt ttctcatcca gtatctcatc
    10381 tactaatgca atcgcgtaat ctgccatgct gatcgtactg tcgccagcag cgtttctttg
    10441 taaataatca tcacttaaca aataagtacc tgtacgtttg ccatgtggat caaataaaga
    10501 agctggactg atataagtcc aatgtagatt tttagattgt ttcaatttta acaatccttc
    10561 gtacatattg aaagctgttg gatagaaggg tgcattttta ggtacttggt caatcatacg
    10621 agtcgtcttt tctgtatcta agaataacga agaggcaccc ccaacgacaa ttaagcgtgt
    10681 atcggttcct tcaagcatct caatcaaatg ggacaaagaa gtttgatgca tttcttcttt
    10741 tcctttggga gcgttgaaag catcaacgac aacatcaaaa gatgcaagat cagtagttgt
    10801 caactcaaat aaatcctttt gtataaacgg aacatctgat ggcaattttt ctggatgacg
    10861 cacgattgcc gtcacgtcta aatctctgga caatgcttcg tctaaaataa atgaacctgc
    10921 atggccagta gctccaatga gtgcaatttt cataaaaaac ctcctttttt attcctgtcc
    10981 attgtaagca gttgctgaat attcgtcaaa gaatccgatt tttgaaaagc tcagtgcgca
    11041 ttcctttcat ggttcgtgat cattgggtat agttaaatta agaaaactga ttgataaaaa
    11101 tagaagttga aggagataag aaatgaaaga gtggttagat cgtgcctttt taccaatgat
    11161 aacatctgtt gtgaatctta gacccttaac tgctttgcgt aatggaatga cttacacgat
    11221 cccaataatt attgttggct cgctgttttt cttatttgct cattttcctg tggattacat
    11281 cgctcagtgg atagacaaaa caggattttc tatgtatttt acacaagtat accaggctac
    11341 atttgatgtg atagcctttt ggatcgtttt tgccattgca tacgcttatg caaaagaaga
    11401 agacatcgat cgcttaccag tcggcataac agccttgtct gcttatctac tgcttattca
    11461 gccactaaat actcagatag atactgtctg gacaggtgct gaagggatac taggagcagt
    11521 tacaactggg ctcatcatcg gatggggcta tgcacgttta gtaagacttg cgaaagggtg
    11581 gaagcaaaga gagaagatac ctgaaaatgt ttggcagtct tttacttcat tgattccaat
    11641 cattgttata gtcactttta cgcttatcgg ttcagcggtt tttcaacagt tgttcagaat
    11701 gactgttgtt caaggagtat ggacatatgt ccagcttccc attcaatcgt tcatagacac
    11761 gttgtttggt gtgcttttat taggtttttt tgttccgttt ttatggttat ttggtcttca
    11821 tggatctgcg atggtcaatg ggttggttag tcccgtcttg caagcgaatt cattagcgaa
    11881 tgcagaaatc ttagcctcag gacaagagtt gacggtggca aatggcggac atatcgttac
    11941 tcagcaattt ctcgatcagt tcatgacggt tacgggtgct ggattgacgt taggggctgt
    12001 attttttatg acggtttttg cgaaatcgaa gaaatacaga gaattaggaa atcttgctct
    12061 tttaccagcc tttttcaaca tcaatgaatc aattattttt tccacaccga tcgtgatgaa
    12121 cccgatgatg gctgttcctt ttattttcgc accggtcgtt tcaggattga ttacctattc
    12181 cgctttatat tttggattcg ttcctttatt tacagcagtc caagtccctt ggacaacacc
    12241 gccgatcctc tcgggtttct tagtaggcgg agtgcctgca gcgattttac aaggtatcgt
    12301 gttgtcaatt agttttttca tttatttccc atttttgaaa aaaatagatt ctgcaaactg
    12361 caaaaaggaa agacagacaa aaaatgatgg agcagcagaa aagatgaacg actaaaaaac
    12421 aaaaatactc ctgtcgcttt agattgggaa aatcaccaat ctaggatgac aggagtattt
    12481 atagtcatgt ttatactagc cctttttgga cagctaattg aacaagaaca actaatgtgt
    12541 tcattcctac gtgtgtgatc atcgatgtcc agatccgacc agttgctttg tactctaggc
    12601 tgaagaagaa acctaggaaa aagtagatta gcagatgacc atcattatga gcaaacgcaa
    12661 acagcaaaga gctgatcact gctccgaccc aaaaatttga atatcgactg atgatcccca
    12721 gtatactgcg gcggaatacg aactcttcca tgatgggtcc agcaacagtt gtcgccaaaa
    12781 taaaagcggg tgcttccatg atcatctgga tgatattttg tgtattctcg gaaggggttg
    12841 ctttgccaaa caatacagat tctaacatga cagcgatgct ttgcagaatc aatgcgacaa
    12901 agattccaac caatccataa ataacaatct ttggagcaga agcttttttt accgctgatt
    12961 cgatcgctaa gggttcacgt tgcttaaaat aaaaaatagc taggacaacg gcgcctagta
    13021 tatacatgac tgcagttgct gtgatcaatt cagacgtaga ttttacaagt cctatcgaag
    13081 ctaggatcaa gggtgagata aaaattagac catagcaaaa aatgctgagg aagctatatt
    13141 tttttaaaga catgtaattc ctccttttaa gggataacaa gcatgcaagg caagcttata
    13201 tcgattgatt tcaattatat cataatttca ataagcagct gtcagaataa aatttctttt
    13261 ctttgattta aaagcttgca aaaaagaggg agaatgagta ttattaaaaa tgtaggttag
    13321 cactcgaagg ttaagagtgc taacagatta aaaaaagagt tatcggaggg atttatcgtg
    13381 ttaaaaccat taggtgatcg cgtcattatc gaagtcgcaa aagaagaaga aaaaactgtc
    13441 ggaggaatcg ttttagcttc tgctgctaaa gagaaacccc aaactgggac agttgttgca
    13501 gtcggtgaag gccgtttgtt agaaaacggt gaaaaagttc ctgccgctgt aaaagcaggc
    13561 gaccaagtta tgttcgaaaa atacgccggt acagaagtaa aatacgaagg aaaagaatat
    13621 ctgatcgtcg caggaaaaga tatcatggcg attgttgagt aaatttggaa gataaaaaaa
    13681 taatcaaaaa taatcaatga ggtgaatcaa tcatggcaaa agaattgaaa tttgcagaag
    13741 acgcacgcgc agctatgtta cgcggggtag ataaattagc agatacagtg aaagtaacat
    13801 taggacctaa aggccgaaat gtcgtactag aaaaatctta cggctctcca ttgatcacaa
    13861 atgatggtgt gacgatcgca aaagaaatcg aattagaaga tcattttgaa aacatgggtg
    13921 caaaacttgt atcagaagta gcatcaaaaa caaatgatat tgcaggagac ggtacaacga
    13981 cagcaactgt tctgacacaa gcaatcgttc gcgaaggttt aaaaaacgta actgcaggtg
    14041 ctaatcctct aggcattcgt cgcgggatcg aattggcaac aaaagcagca gtcgaagaat
    14101 tgcataatat ctcaactgtt gttgattcaa aagaagccat tgcacaagta gcagctgttt
    14161 cttcgggttc tgataaagtt ggacatttga ttgctgatgc aatggaaaaa gttggaaacg
    14221 acggtgtcat cacaatcgaa gaatcaaaag ggatcgaaac agaactagat gttgtagaag
    14281 gtatgcagtt tgatcgtggt tacttatcac aatatatggt aacagataac gacaaaatgg
    14341 aagctgttct agaaaaccca tatatcttga tcacagacaa aaaaatctct aacatccaag
    14401 atattctgcc attgttagaa caaatcttgc aacaatcacg tccattgttg attattgctg
    14461 acgatgtaga tggtgaagca ttgccaactc ttgtattgaa caaaatccgt ggaacattca
    14521 acgtagtagc tgtaaaagct cctggtttcg gcgatcgtcg taaagcaatg cttgaagata
    14581 tcgctatttt gacaggtggt acagtaatta ctgacgatct aggtttagaa ttgaaagatg
    14641 taacaatcga aaacttagga aatgcttcta aagttgttgt agataaagac aatacaacga
    14701 tcgtagaagg ttctggagaa aaagaagcaa tcgaagctcg tgtgcaattg atcaaaaacc
    14761 aaattgctga aacaacttct gattttgacc gcgaaaaatt acaagaacgc ttagctaaat
    14821 tagcaggtgg tgtagctgtt gttaaagttg gtgcagctac tgaaacagaa ttgaaagaat
    14881 taaaattacg cattgaagat gcattgaatg ctacacgtgc cgcagtagaa gaaggaatgg
    14941 tctcaggtgg tggtacagct ctagtgaacg ttatcagcaa agtttctgct gtagaagcag
    15001 aaggagacgt ggcaacaggt atcaagatcg ttgttcgtgc tttagaagaa ccaattcgtc
    15061 aaatcgctga aaacgctggt tatgaaggat cagtgattgt tgataaattg aagaacgtgg
    15121 aactaggcac tggtttcaat gctgctactg gagaatgggt aaacatggtt gaagcaggta
    15181 tcgtagaccc aactaaagta acacgttctg ccttgcaaaa tgccgcttcc gtttctgcct
    15241 tgttattaac gacagaagca gtcgttgcag ataaaccaga accagcagcg ccagcagctc
    15301 ctgcaatgga tccatcaatg atgggcggta tgatgtaaga aaaagcaact tgcttatcta
    15361 caactgatca ttagtgaaag acagataaaa agaggctgga acagaagcgt ttaactccaa
    15421 gaaataagaa gaaattcacg gaaattgctt ttcaaatttt tgtgaatttc agcttatttc
    15481 cgaaggagtt gcttctgctt ccgctgttta tacgtgttta gagcgtgaga caaaagtgtt
    15541 ctttactttt gtcccacgct cttttttgtg tgtaattata atgaaaatag ttatttttaa
    15601 tagttataat attttatcta ttaaaagtaa atatccattc a
//
LOCUS       DAZBTF010000063        15296 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000063 DAZBTF010000000
VERSION     DAZBTF010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.063"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(93..1136)
                     /locus_tag="ACHGNS_002132"
     CDS             complement(93..1136)
                     /locus_tag="ACHGNS_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFN6938056.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(1513..2478)
                     /locus_tag="ACHGNS_002133"
     CDS             complement(1513..2478)
                     /locus_tag="ACHGNS_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFN6938057.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            2712..3557
                     /locus_tag="ACHGNS_002134"
     CDS             2712..3557
                     /locus_tag="ACHGNS_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN6938058.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            3748..4935
                     /locus_tag="ACHGNS_002135"
     CDS             3748..4935
                     /locus_tag="ACHGNS_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFN6938059.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            4941..5297
                     /locus_tag="ACHGNS_002136"
     CDS             4941..5297
                     /locus_tag="ACHGNS_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFN6938060.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            5299..5637
                     /locus_tag="ACHGNS_002137"
     CDS             5299..5637
                     /locus_tag="ACHGNS_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFN6938061.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    5713..5969
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6032..7066
                     /locus_tag="ACHGNS_002138"
     CDS             6032..7066
                     /locus_tag="ACHGNS_002138"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFN6938062.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            7059..7745
                     /locus_tag="ACHGNS_002139"
     CDS             7059..7745
                     /locus_tag="ACHGNS_002139"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFN6938063.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            7769..8617
                     /locus_tag="ACHGNS_002140"
     CDS             7769..8617
                     /locus_tag="ACHGNS_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFN6938064.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEEKPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            8814..9587
                     /gene="sufC"
                     /locus_tag="ACHGNS_002141"
     CDS             8814..9587
                     /gene="sufC"
                     /locus_tag="ACHGNS_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFN6938065.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            9600..10886
                     /gene="sufD"
                     /locus_tag="ACHGNS_002142"
     CDS             9600..10886
                     /gene="sufD"
                     /locus_tag="ACHGNS_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFN6938066.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            10883..12118
                     /locus_tag="ACHGNS_002143"
     CDS             10883..12118
                     /locus_tag="ACHGNS_002143"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFN6938067.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            12105..12575
                     /gene="sufU"
                     /locus_tag="ACHGNS_002144"
     CDS             12105..12575
                     /gene="sufU"
                     /locus_tag="ACHGNS_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFN6938068.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            12580..13971
                     /gene="sufB"
                     /locus_tag="ACHGNS_002145"
     CDS             12580..13971
                     /gene="sufB"
                     /locus_tag="ACHGNS_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFN6938069.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(14069..15211)
                     /locus_tag="ACHGNS_002146"
     CDS             complement(14069..15211)
                     /locus_tag="ACHGNS_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN6938070.1"
                     /translation="MATFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAE
                     AQTALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILP
                     AFGELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTL
                     LPRKIEEEQPLKFYTKDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWED
                     IDYFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMI
                     LYGYNTNSPKQFLFTTNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLF
                     ESGASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
BASE COUNT         4999 a         2848 c         3263 g         4186 t
ORIGIN      
        1 gttgttgtta gaaggatatc tgcaaaatta aaaacagttg tgattcggac attgttccat
       61 gcgagaatca caactgtttt ttcatgttca ttctaaactt tgatcagcca aataaatagt
      121 aaagccatca ctgtttggag aagcccgaca gctaatccat aagataagaa gcgtgtacct
      181 tctttcatga attttgcaaa gtctaaacgc agaccaattg ccgctaaagc agtgatctca
      241 aaccagctgc tgataaaatg tgccgattgg tcaataacta caggtaaaga aaacaagctg
      301 ttgaccaaac aaaagatcac aaaccctaat acataccaag gaagcggtaa ttttcgtttt
      361 ttctgcgtat cagttggcgt ttcaaacaga gctggttgtt tcttgtgcat gaatttttca
      421 aacagataga caacaacaac gagcatcata atacgtagaa tcttaaatag catggcgaat
      481 tcgacaacag aattgtcaag taagcttgcc ccagcgacta cttgaccaac agattggagt
      541 gtaccaccca aaagtgcact tttttcaatg agatttccac caaacaactg taatccaaga
      601 aaagggagag tcaacatcat cacagtaccc agtagattga ctagtgtgat gatctgtcct
      661 ttttcttcct ctttcgcatg gatggaaggg gcgattgaag caatagcaga agaaccacaa
      721 acagcgtttc ctccagccat catcaaagaa gaagtatcgc tgaattgcag ttttttacca
      781 atcaagtaag caccgatgat agtgactgac atttgcagta acacaaaaac aattcctttt
      841 acaccaattt gagcaatcgt ttgagctgtg acagttgcac ctagtaaaaa aacagagaat
      901 tctaatagtt tgctttcagc aaattttgtg ccgattgcta gaacaggctg tttaaaaaaa
      961 gtatttccta ataaaatacc caataaaata gcaatagtcg ctccacctaa ctgtggcaga
     1021 taaatagcaa gaagcctgct gaaggcagcg atggcaaaag aaagcagtaa accgggtaag
     1081 atttcgcgga ccgctgaatc ctttacagat tttattggat gtgacaatga tttcatttga
     1141 tcggttcctt tcgtcaataa atactaaaag catactcgaa catagtagca ctaaaccagt
     1201 attttgacaa aagaacaaga ggtcgtgaca aaagtcttgt acgacctctt gtttcgaata
     1261 aacggtgttc cagaagtagc ttctacggaa ataagccgaa atttcacaaa aatttgagaa
     1321 gcaattttcg taaattcctt cttatttctt gaagctgacc acttctttta cgacctcttg
     1381 gtaaacggtg ttcaaaagct tgcatctgcg gtaataagcc caaccagtac aaaaacatga
     1441 acagctgttt ttgtttggcc gttcttatta cttgatgcag aacagctttt tcacaaccta
     1501 ttgaatcgat tattaagcga tttttttaga aacaaatttt ccatgtttgc tatgttcttt
     1561 aattaagaaa cgtacgtaat gagcaagaac gtagaagaca actacacttg caaacagaca
     1621 ttccgcaact tttaagaaat ccaatacgct actatagata cctaaactgt tgaagtaatg
     1681 aacagtagta aatatagctg ttgcagagat aacaagcggg aatgtaaagg ctgcatagct
     1741 tggataaaaa cgaagcttga acaaacgtgg aagcatagac agcacaacga aatacaatac
     1801 ttgagataaa gcgaacaatg ctacgacaag ataaaaatta ggttcagcaa agtttttaag
     1861 ataacctgtt aggcaaagag aaccaggtgc tgcaacgatc gtaatcagcg gacgtgtcgg
     1921 atgagccata tttcttacga aaaagacacg tttgatgatg atcggcaata agaacgcata
     1981 gcagatcaat gaaatccaaa agaaaatttt tccaataagt gggaagaagt tcccggcagt
     2041 tacagtgatg accccaaatc caacgtaaat gataaaccag cttggataaa ttgcttcaat
     2101 tgaaagatca gctttgataa catggtgata attgaaataa aagacaagga tcacttggaa
     2161 gataacagcg cacagccaaa tgaatttgac aattggtgca atactttcat atgaaacaaa
     2221 ataagtacaa ataaccatga gtgacatcgt aaaagttggt gaaacggagg cgatgattgg
     2281 atcatgcaac gtttgtttcg tgtgttcgaa taagataaac aatttaacaa aaatgaatat
     2341 cataagaatc atagagatac cgccagttag attgcctaaa gcaacatggt gatagtcttt
     2401 aaacagattt ccaagggaag ctaacccaag gatcaacccg cagatcggga tcggaataat
     2461 tttcaaaaag ttcttcatat ataatattct cctttttatt taatagattt tagtaccaag
     2521 atgaacgaat acattgtaaa atgaatagga atgtatgatt acgagcaagc agacagctga
     2581 ttgtcttcat ctaagttact agttggatca gcaactgttt tgttgtacat ttctattata
     2641 gatggttttt tgtatattta aatcaaatag tttttatagt aattatcata aaaagttatg
     2701 gaagtggtgc catgtttaag ttattaacga cttttcgagc agtatatgaa acaagaaatt
     2761 tttccagagc agcagaagta ctgtttttat ctcaaccagc agtctcgaat caaatcaaac
     2821 agttggagcg ggaattgaat atcgatcttt ttcaacgtaa cggaagaaaa gagatgcttc
     2881 caactaagca agccgactta ttatatgaac ggacactggt tttgttagaa gaatgggaag
     2941 aaactcagca aaaattgctc aatgaacaaa atgaagaaga agtatgtcgg atcgttgctt
     3001 cccacacctt tgctgtctat cttttgcctc agttgatgaa acatctgatc aaaaggtttc
     3061 ctaaagtgca ttttgagatc gaaatcacga attctcatga atcattagaa caagtagcga
     3121 aacatgaatc cgattttggt tttatcgaaa agccattaga gacttccggc gtacaaagat
     3181 actctttgat ggaagaccag ctagtcatcg ctggggaccc aaaaagcaaa ttgtggcttg
     3241 taagagaagc aaactctggt gtttttcatt acacgcagcg ttattttgaa gagatgaata
     3301 tccaagataa gaagatgatc gtgaaaagta acgatgtaat tgttgcgttg ctgaaggaag
     3361 gaatcggtcg ctcgattttg tctaagcgct ctgttccaga aaatgtgcca atgactccgt
     3421 tgaatttcca tcgatatttt tatttcatcc aacgaaccaa tctggtttct gatcagctgc
     3481 tagagattgc cgaagagatc cttcgataca atgaaagcac agaaacttct cacagcgagg
     3541 agaatctcag ctggtagata attgcatgta tcaaaaaaag ttctatgata cactaaagat
     3601 aagcagtagg cttttgtact gcatttgctc aagcaactgc acaaacggaa agttaatttt
     3661 tcccaaatgt ctatggaaag actttccatt atctaactgg gccgctataa taatcagcag
     3721 tatggttttt aagaaaaggg atgaaacatg gaaaaaaaat taaaagcttc gaacgataat
     3781 cgtattgatt atggcgtgat tttaccagtc tttctgttat gtttgattgg tctgctctca
     3841 ttatatgtag cacttagcca tgatccgaac aatccagcag tcttgaaagg cgtagggatg
     3901 caagttgtct ggtatttagt tggagcggca gcaatcgttg tcatcatgca tttgaattcc
     3961 aaatggatct ggaaactgac tccttacctt tatggtttgg ggttagtggt aatgggactc
     4021 ttgctcaaat tctatgatca gaatttagct gcttcaacag ggtctaaaaa ctggtttcga
     4081 ttcggtgcgt ttactttcca gccagcagag ttgatgaaga ttgcgtacat cttgatgatg
     4141 gcactgatcg taactaaaca taatacgcaa gtaaaagaac ggacgatgaa atcggacttt
     4201 tggctgattg ggaagctctt gatcgtgaca ataccggttt tagcattgat catggcccaa
     4261 gatgactttg gaacgatgct tgtctttctt gcgatctttg gcgggatttt cctgatgtct
     4321 ggaatctctt ggcgcatcat cgcacctgta gtaatcttgg cagtagtcgt tggagcagga
     4381 acgatcttct tggttacgac tgaaggcgga agagacttgc tttataaagt tggattcaaa
     4441 tcttatcagt ttgcacgtat tgattcatgg ctggaccctt tccatgatac gtcaggaatg
     4501 agttatcagc cggcccaagg acttttggca attggtaccg gtggcttgtt tggtaaagga
     4561 ttcaacgtaa gcaatatcta tgtgcctgtt cgtgagtccg atatgatttt taccgttatc
     4621 ggtgaaaact tcggctttat cggtggtgca ttcgtcattt tcttgtattt tattttaatc
     4681 tatcgtatga ttcgggtgtg tttcgatacg aataatgaat tttacgccta tatcgcaagc
     4741 ggtttgatca tgatgctgct gttccatgtc tttgagaata ttggagcaaa tatcggatta
     4801 cttcctttaa cagggatccc attgcctttc attagtcagg gaggatcctc gattttaggg
     4861 aacatgatcg gtatcgggtt gatattatcg atgagatacc aaaatgaagc acctgcaaga
     4921 tcttcaagga ggtaagaaaa ttgtacacct tttactggta cccgaaatgt tcaacatgca
     4981 aaaaagccaa agcgtggtta gaccagcatg gcgttagtta tcaaaccgtt gatatgatca
     5041 aagaagtacc gaaggcctca acgattgaga cttggctaga aacgagtcaa gtaccaatcc
     5101 gtcgcctgtt caacacaagc gggatgaaat atcgggaact tggactgaaa gatcaagtag
     5161 gcgagtttac ggcttcaaaa gcgagccaag tcttatcgac agatggtatg ctgatcaagc
     5221 gaccactgat cttacgagag gaccagcttg ttgcaattgg attcaatgaa aaaatttatg
     5281 aaggagaact gatataaaat ggcaggcaag catttgaaga aaaaggacaa cctttggatc
     5341 ttatttaatg gaaaagaata ctgtgttggt ttgacaaatg aagcgcaaga agaacttgga
     5401 gacatcacgt ttgccaatct gccaaaagca ggacaatcgc taaaaaaaga tgaacctctg
     5461 atcgaagttg aagcggaaaa agcggtcaat gaatttgcca gcccgctaac gggtacagtt
     5521 tcttctgtta atgataaaat cggtgaagat atcaatatct tgaatgattc agatgaaatg
     5581 aatgcgtgga tcttaagttt caaagatgtt gatccaaaag aatttgacga attataaaat
     5641 cagaaaatca cttgaaaaaa gttgacactg attcttccag ttgctataat cataagcaat
     5701 cctatatgaa acgagaaagc tttgaaaaga agagtacatg tcacggacgt ttgaagagag
     5761 ccctgtttgc tgaaaatggg taacgaaaat gagatggaag atggtcttag agcagagtaa
     5821 gcgagcgtaa gcaaacttac tacgggagtg cccgttacag cgcgacagta tacggaaaga
     5881 agctaagtcc aagtactgga agaggctgtt attgcgaagt aacggtaaat caaggtggta
     5941 acacggaagt tcaagctttc gtccttttgc ttaaggcaag agggcgaagg cttttttgtt
     6001 tgaaaaatta taagagaaag atgtgaatgg catgtcatta atccaactaa cgaatgtcaa
     6061 aaagacattc aatgggaaaa acggaaacat ccaagccgtc aatgacgttt cactaaacgt
     6121 tgaaaaaggg gatatttatg gaatcgtcgg ctattctggc gcaggaaaaa gtacattagt
     6181 ccgtctcctg aatggacttg agcttcccac gagcggcgaa gtgatcgtaa acgatcaaga
     6241 tattacaaaa ttaaaaaaca aagaattacg tacattccga aagaaaatcg gtatgatctt
     6301 ccagcatttc aacctgttat ggtcaagaac tgtacttgaa aatatccagc tgccgctgga
     6361 attagctggc gtaccaaaga gcaaacgtaa agaacgggca gaagaactgt tgcgtttagt
     6421 cgggttagaa ggacgcggac aagcatatcc ttcacaactt tccggcggac agaaacaacg
     6481 cgtcggtatc gcacgagcat tggcaaatga tcctgagatc ctgctttgtg atgaggctac
     6541 tagcgcattg gacccgcaaa caacagaaga agtattagac ttattgttag cgatcaataa
     6601 gaaattgaat ttgaccatcg tcttgatcac acatgaaatg aatgtgatcc gcaaaatctg
     6661 caacaaagtc gctgtgatgg agttggggaa agtcgtagaa gaaggagacg tattgactgt
     6721 cttcagacaa ccgaaaaaag aagtaaccaa acgctttgta caacaagaca tcgaaccaga
     6781 agctgattcg acagaactat tagctgaact catcaaagaa aatccaagcg gccgtttggc
     6841 aactttgacc tttagcgaaa gaaatgccaa cgagccagtc atttcccaag cgattcgacg
     6901 ctataacatc gatgtcaacg ttgtgtatgg aaagatcaaa caagtgaaag aaggttcctt
     6961 tggttcattg actgtcttga tgactggtga aaccaaagaa ttagatcaag cagaagtgtt
     7021 catcaaagaa caaggagtag gaattgaggt gatccatcgt ggataaaagc ttttcagaaa
     7081 catatctaga ttttaaacaa atcaacccgg aaacgatgaa tcaggctatc cttgatacac
     7141 tatatatgac attaatctca atggtcttag tgacgatcat cggattagtt ttgggattga
     7201 tcctttactc tattggacgc aaaaacaaac cgtatgctcg aatcatttat ggaatcgtat
     7261 cgatcatcag taatattttc cggtcaacgc catttatgat cctgatggtt ttgatcattc
     7321 catttactaa agcaatcgta ggcactatgc taggtgcgca agcagccatt ccagctcttg
     7381 ttttatccgc agcaccattt tatgcccgtc tagtagagat tgcttttcgt gaagtcagcc
     7441 ctggtgtact ggaagcagca gatgcaatgg gagcaagtta ctgggaaatc atctggaagg
     7501 tcttgatacc tgaaagtatt cccgcattga tttctggatt aacagttaca acgatctcga
     7561 tgatcggatt tactgcgatg gccggcgcga tcggcgcagg tggacttgga ggtcttgctt
     7621 ggcaggaagg ctaccagcgt ggtaatctaa cagtgacatt cgttgcgact ttaattattt
     7681 tactaatcgt atttatcgtt caaggaatcg gtgatttctt aactaagaaa acagacaaaa
     7741 gataatagga tataggggga tgtaggatat gaaaaagaaa atttttggat ttgcagcagc
     7801 gcttttgctg actgtaggat tagcagcatg cggtaacaac agcgacagca aagattcagc
     7861 aaataaagca gacgacacga cattgaaagt cggcgcttca ccgacaccgc acgcagagat
     7921 tttggaacat gtgaaaccat tattgaaaga tgaaggaatc gatcttgaaa tcgtcaaatt
     7981 cgatgactat gtattaccga accaatcttt ggaagaaggc gacattgatg ccaattactt
     8041 ccagcatatt ccttatttga acaaagaaat caaagaaaaa ggatatgact ttgtcaatgc
     8101 cggtgctgtc cacatcgaac caatgggctt gtactctaaa aaagtcaaag atgtttccga
     8161 attaaaagat ggtgcaacaa tcattacttc aaattctgaa tcagactggg gacgcatcat
     8221 cacaattcta caagatgctg gcttagtcaa agtcaaagat ggagtagatc tggaaacagc
     8281 aacattcgat gatattgaag aaaaacctaa gaacttgaag ttcaaccata cgatcgaccc
     8341 atcactttta gcttctacgt accaaaatga tgaaggtgac ctagttgcaa tcaacgcaaa
     8401 cttcgcttac ggcgcagggt tgaatccatt agaagatgcc gtattgcttg aaaaggacaa
     8461 ctctccttac gtgaatatta ttgcgactcg taaaggtgat gaagacagtg acaaaatcaa
     8521 aaaactgatt gaagtgcttc accgcaaaga agtccaagaa tggatcacag acaaatgggg
     8581 cggctctgtt aaaccagtaa aggctgatgc aaaataaggt atggaacaaa aaagatgaga
     8641 aataagtctg taatggctta tttctcatct tttttatacc tattctaatt tagaaatcaa
     8701 aattgtttaa taataaataa aagatatttg attttagtaa ggaaggtcat tgcgcttcat
     8761 tctcaattag tctaaaatga gtgagaacga aagagaattt gggggtataa tgcatgtctg
     8821 tcttagaaat aaaaaatcta catgtgtcga tcgaggaaaa agaaatcctt aaaggcgtaa
     8881 acctaacaat gaaaaccgga gaaatccatg ccatcatggg acctaacggc acaggaaaat
     8941 ctaccttatc tgccgctatc atgggcaatc caaactatga agtaacagaa ggcgaaattt
     9001 tgtttgacgg gaaaaatgtt ttagacttgg aagtagatga acgtgcgcga cttggcttat
     9061 ttttagctat gcaatatcca agtgagatac caggtatcac gaatgcggaa ttcatgcgtg
     9121 cagcgatcaa tgcgaaacgt gatgaaaacg acaaaatttc tgttatggca tttttgaaaa
     9181 agttagatga aaaaatggct ttactaaata tgccagaaga aatggctgaa cgttatctga
     9241 acgaaggttt ttctggcgga gaaaagaaac ggaatgagat cctgcaattg ttgatgttag
     9301 aacctacatt tgcaatttta gacgaaatcg attcaggtct ggatatcgat gctttgaaag
     9361 tcgttgctaa aggagtaaat gaaatgcgcg gagacaattt cggtgcattg atcatcaccc
     9421 actatcaacg attattgaac tatatcacac ctgatgtcgt tcacatcatg atggaaggcc
     9481 gagtagtcat gacagggaat gctgacctag ctaaacgtct ggaagcagaa ggctacgctg
     9541 gtatcagtaa agaattagga atcgactata aagaagaaga agcgtaaggg ggagcaagaa
     9601 tgaaagaaaa aaactggctc gattatcttg acgcggtaaa tgatttttct ttaagtaaag
     9661 gcgaacctga ttggatgaga accttccgtc aagacgcact agcgaaagca gatgaactgc
     9721 cattgccgca tatcgatcgg gtgaaattcc atcgctggtc gttatttgat gtgaaagaaa
     9781 cacaaaccat cagtgaaaca ggcacgattc cggcatttga tgcaatgaaa gacaatcctg
     9841 tgcttgtcca acaaggttca tggacgattt ttgaacaact gcctgtggaa ttagcagaaa
     9901 aaggagtcat ttttaccgac ttgttcactg caatgatcga atatccagaa ctagtccaag
     9961 aatattatat gaaaaaagca gtaaacatga acgaagacca attgactgca ctccatgtag
    10021 cttttatgaa cagcggtatt ttcctgtatg tgcctaaaaa tgtcgtgatc gatgaaccac
    10081 tcgagtcctt attcattcaa gatggcgcat ccgatgagca cttcttcaag catgtgttga
    10141 tcgtggcaga tgaacatagt gaattttctt atctggaacg tttccagaca accaaagaac
    10201 aagtagcaaa atcatcaggt aatatcatcg tggaagtcat cgctaaagct ggttcaaaaa
    10261 tcaaatattc agccgtagat caattaggag aaaatattac ttcttatatg aatcgccgtg
    10321 ggcacatttt acgagatgct tctgtcgact gggcaattgg tgtcatgaat gacggtcatg
    10381 tgatcgctga ctttgactca gatttagcag gagaaggtgc acacgcagaa gtcaaaatcg
    10441 tggcaatcag ctcgggcaga caaatccaag gaatcgatac acgagtaacc aataaagccc
    10501 ctcatacgat cgggcatatt ttgcaacacg gagtgatccg agaaaaagga acattgacct
    10561 ttaatggtat cggccacatc ttgaaagggg caaaaggtgc agatgcccaa caagaaagtc
    10621 gtgtcttgat gctttctgat aaagcacgtg gtgatgcgaa tcctattctt ttgatcgatg
    10681 aaaacgaagt aactgctgga catgctgcca gtgtaggacg agttgatcca gaagaaatgt
    10741 actatctgat gagtagagga ttgcataaag aagaagcaga gcgcttagtg attcgaggat
    10801 tcctcggttc tgtcttaaca gcaatccctg tagaacaagt tcggaaagaa ttagtcgaag
    10861 taatcgaagg gaagttgaat ggatgatcga tgctaaaaag ctacgcaatg attttcctat
    10921 cttatttcaa gacgtcaatg atgaaccttt agtttatctg gataatgcag cgacgaccca
    10981 aaagccaaaa caagtactcc aggcattagc gtactattac gaacacgata acgccaatgt
    11041 tcatcgcggt gtccatacgt tagcagaacg cgcgacccat gattatgaac aagcaagaga
    11101 aaaagtacgg gcgttcatcc atgcaaaaga aacagcagaa gtgctgttta ctcgtggtac
    11161 gacgacaagc ttgaattggg tagccagaac cttcggcgac cgctatatca aagcaggaga
    11221 cgagatagtg ctctcttaca tggaacatca ttctaatgtt atcccgtggc agcagttggc
    11281 aaaacgaaca ggtgctgtct tgaaatatat cgaactaaca gaagaaggct tcctggatat
    11341 ggaggatgca gcgaaaaaga ttacggacaa aaccaaaatc gtctccattg ctcatgtatc
    11401 caatgtctta ggtgtcttca atcctgttca agaattagct gaacttgtcc atgaaaacgg
    11461 cggggtcatc gtagtagatg gtgctcaatc tgccccgcac atggccattg atgtccaagc
    11521 actagactgt gatttctttg ctttcagcgg acacaaaatg tgcggaccaa caggtattgg
    11581 tgttttatat ggtaaacgaa aatggttaga agaaatggaa cctgttgaat ttggtggcga
    11641 aatgatcgat tttgtcgaat tatacgacag tacatggaaa gaactacctt ggaaattcga
    11701 agcagggaca ccaaatatcg ccggcgctat cgcattaggc catgcaatcg attatttgca
    11761 aaaactcggt atggacaata tccatcgata tgaagaagaa ctagccgctt atgtgttgcc
    11821 aaaattacaa gcaattgatg gattgactgt ttatggtcca caagacccga attttcggac
    11881 aggagtcgtt gcattcaatt tggatgggtt acatccccat gatgtcgcga cagctttaga
    11941 catggaaggg gtagccgtca gagcaggtca tcattgtgcg caaccgctat tgaagtacct
    12001 tgaagtatca gcgactgctc gcgcgagctt ttatttctac aacacaacgc aagacgccga
    12061 ccgtttagtt gaagcaatca tggcaacaaa ggagtttttc caacatggga ctatctaaat
    12121 tagacaattt ataccgtcaa gtgatcttgg atcattcttc ccatccgcat catcgtggaa
    12181 gcttgactga ctcgacaagc aagatcgaat tgaacaaccc cacatgtgga gatgtgatcg
    12241 aattgcaagt cgatgtaaaa gacggaatca tcaaaaatat cgcctttgac ggcagtggct
    12301 gctccatcag tacggcaagt gcctcgatga tgacagacgc cgtcatcgga aaatcaatag
    12361 aagaagcaga acatttaact gtagactttc tacagcttgt ccaaggaaaa gaagtcaaag
    12421 atcccgacca attaggcgat gctgctatgc ttggtggtgt agctaaattt cccgctcgga
    12481 tcaaatgtgc gacacttgcc tggaaagcac taggaaaagc aatggaaaac gatggtaccg
    12541 cagctttcca tcattaccac aaggaagagg agtgagaaca tgggcgtacc agaattagaa
    12601 gaatataaat tcgggtttca cgatgatgtc gaaccggttt tcagtacagg agaaggcttg
    12661 acagaagaag tgatccgtga gatgtcaaga atcaaaggcg aaccagaatg gatgcttgaa
    12721 ttccgtctga aatctctaga aacatttaac aaaatgccaa tgcaaacatg gggccccgac
    12781 ctttcagaca tcgatttcga tgccatcaaa tattaccaaa aaccaagtga caaacccgct
    12841 cgtgactggg aagacgtacc agaaaaaatc aaagatacat ttgaaaagat tggtatacca
    12901 gaagctgaac gagcttatct agccggtgct tccgcacaat atgaatcaga agtcgtgtac
    12961 cataatatga aagatgaatt cgaaaagtta ggaatcgtct ttactgacac tgactctgct
    13021 ttgaaagaat accctgaatt attcaaagag tacttttcaa aacttgtacc gccaaccgac
    13081 aacaaattag cagcgctgaa ttcagccgta tggtcaggtg ggacctttat ttacgtgcca
    13141 aaaggcgtca aagtcgatgt tccattacaa acgtacttcc ggatcaatgc ggaaaacacg
    13201 gggcaattcg aacgtacgct gatcattgtt gacgaaggag caagcatcca ctatgtcgaa
    13261 ggttgtacgg cacctactta ttcaagtaac agcctgcatg ccgccatcgt agaaatcttt
    13321 acaagaaaag atgcgtattg ccgttacaca acgatccaaa actggtcaga caatgtgtac
    13381 aacttagtaa ccaaacgagc aaaagcatac gaaggcgcaa ccgttgaatg gatcgacgga
    13441 aacctaggtg caaaaacaac gatgaaatac ccaagtgttt acttagatgg aaaaggtgca
    13501 cgaggcacca tgctctccat cgcctttgca ggagcaaacc aaatccaaga tacgggtgca
    13561 aaaatgatcc acaatgcacc aaatacatct agctctatcg tctctaaatc aatttcaaaa
    13621 gacggcggag aagtaaacta tcgaggacaa gtaacctttg ccaaaaacag tgcgggttcg
    13681 atctctcata tcgaatgtga cacgatcatc atggacgaat ggtcgaaatc cgatacgatc
    13741 ccattcaatg agatccacaa cagccaagtc tctttagaac acgaagccaa agtctcaaaa
    13801 atctcagaag aacaactcta ctatctgatg agccgaggcc tgaccgaatc tgaagcaact
    13861 gaaatgatcg tcatgggctt tgttgaacca ttcaccaaag aacttccaat ggaatatgca
    13921 gtcgaactca atcgattgat cagttatgag atggaagggt ctgtaggtta ataactattt
    13981 cattgctcga aacgttgata ttaaagcgtt tcgagcgttt ttttatttgt attttaatgg
    14041 ttggctacgt ttttggctac gtttggaatt attcgcccat aaattttgca aatcggtcac
    14101 cagttttctt aacagattgt ggtgtgacat gagcatagat attcatggta gttttaatat
    14161 ctttatgacc taatcgttcc tgtacttctt ttatcgacgc accactttca aacaataagc
    14221 tacagtgggt atgcctaaat ccatgtggag taatttgagg ctccatcggt gtctttttgt
    14281 aaatccagtc taaccaatcg tttactactt gggggtagta caacttgttt gtgtttgtag
    14341 tgaaaagaaa ctgttttggt gaattcgtgt tatagccata taagatcatt attttcattt
    14401 gttcttgttg ccaaatacgc agttgtttaa gtgtaaaatc atcaagctga atcgttcttg
    14461 atgaattttt agttttaggt acttgtagaa ctacttgatt aaactcgtct tgagcgatag
    14521 ttttaccaat tgttattgtt ttattgaaat agtctatatc ttcccattga agcgccaatg
    14581 cttcgctttt acgcattcct gtataagcta ataaacggaa aaaagtgtac agtttaactg
    14641 aattattttc tttagtattc tgtaaaaaca gttgtaattg atctttcgtg taaaacttca
    14701 agggttgttc ttcttcaatt tttcttggaa gcaatgtttt agacatagga ttatccttac
    14761 agacttccat tgctactcca aatttaaata tttgagcagt tgtttttctc aagaaaccat
    14821 attgttttaa tgttttgtgc cattcattca cctgtttttg gcaatagcta acacttattt
    14881 tatccaatcg taattcccca aaagcaggta atatatggtt tttgcaaaaa cgttgagcaa
    14941 cggcaacaga agacggtttt actcttgttc tgtagttatc aatccataat tcatacaact
    15001 cttgaaaagt tgtaacattg attttaaact cttgtttttc tttaaagtct acaagtagtc
    15061 tattaagtgc tgtttgcgcc tcagcttttg atttgaatct tctacgcttt gtacgtatat
    15121 cttttccagt cttgggatct ttccctaaat aagtttcaaa tccccaatat ttttcgccat
    15181 tttgtaattt ataactaaaa aatgttgcca tttgattctc tccttacttc attattgcag
    15241 gtagagaagt tatttgaaga atcaattatt tttaaattta tcgtgattat cagtta
//
LOCUS       DAZBTF010000064        15259 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000064 DAZBTF010000000
VERSION     DAZBTF010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.064"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(231..611)
                     /gene="yajC"
                     /locus_tag="ACHGNS_002147"
     CDS             complement(231..611)
                     /gene="yajC"
                     /locus_tag="ACHGNS_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFN6938071.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(708..1853)
                     /gene="tgt"
                     /locus_tag="ACHGNS_002148"
     CDS             complement(708..1853)
                     /gene="tgt"
                     /locus_tag="ACHGNS_002148"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFN6938072.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            2071..2769
                     /locus_tag="ACHGNS_002149"
     CDS             2071..2769
                     /locus_tag="ACHGNS_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFN6938073.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2937..5576)
                     /locus_tag="ACHGNS_002150"
     CDS             complement(2937..5576)
                     /locus_tag="ACHGNS_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFN6938074.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6195..7226)
                     /gene="queA"
                     /locus_tag="ACHGNS_002151"
     CDS             complement(6195..7226)
                     /gene="queA"
                     /locus_tag="ACHGNS_002151"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFN6938075.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7419..8594
                     /locus_tag="ACHGNS_002152"
     CDS             7419..8594
                     /locus_tag="ACHGNS_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFN6938076.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8598..9239
                     /locus_tag="ACHGNS_002153"
     CDS             8598..9239
                     /locus_tag="ACHGNS_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6938077.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9236..10153
                     /locus_tag="ACHGNS_002154"
     CDS             9236..10153
                     /locus_tag="ACHGNS_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN6938078.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10150..10821
                     /locus_tag="ACHGNS_002155"
     CDS             10150..10821
                     /locus_tag="ACHGNS_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6938079.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11362..11850)
                     /locus_tag="ACHGNS_002156"
     CDS             complement(11362..11850)
                     /locus_tag="ACHGNS_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFN6938080.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(12051..12317)
                     /locus_tag="ACHGNS_002157"
     CDS             complement(12051..12317)
                     /locus_tag="ACHGNS_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFN6938081.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12317..12580)
                     /locus_tag="ACHGNS_002158"
     CDS             complement(12317..12580)
                     /locus_tag="ACHGNS_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFN6938082.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            13101..13241
                     /locus_tag="ACHGNS_002159"
                     /pseudo
     CDS             13101..13241
                     /locus_tag="ACHGNS_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(13383..15206)
                     /locus_tag="ACHGNS_002160"
     CDS             complement(13383..15206)
                     /locus_tag="ACHGNS_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFN6938083.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
BASE COUNT         4427 a         3256 c         2866 g         4710 t
ORIGIN      
        1 gtaaacactt ttttgtgatt tttttcacag ttatttttaa atataaaatt gctttattta
       61 caggtgtttg tatagaaaaa tatataaagt tatttattat tatttatatg acacacccga
      121 ttattcaata ttgagcaata tatcccatgc tcttatctct ttcttttttg aataaaaaat
      181 gaggtaggac atctttctgc cctacctcgt ctttaaataa gctttgatac ttatttttct
      241 tccttctctt ctgtctcttc cagcgtttct gttgtttcag tttttacttc aactgtctca
      301 gctggttctt ctgttgtttc aacagtagtc gttgcatcat ttgttacgac agtccctggt
      361 ttgaccgttt tgattgctgc acgatcgtac tcaaggaaga tcccttcgca gtctaaaaca
      421 actgtccgtt tgtcattgtc gatttctgaa acaactccat gtaatccgcc gatcgttacg
      481 actgaatcac ctggtttcat gctatctaac aagttttgac gttcttgctg ttgtttcttt
      541 tgcgaacgtg acataaaaaa ccacattgct aatagcgcga taaacatcac aagcattggt
      601 aatgttccca tttttttcac ctcactaatt ctgtatctac aaaaaacact ttagcagaaa
      661 aatgctgata acactagttt tttacttaat ttttaaaaga ttttccttta gaagcttttt
      721 gcgttttctt tattgaaccc gtattcttca aaaaaggctt ctctaaattc aagcaaatta
      781 tcatccatga ttgcttgacg tacttgtttc attagattca acaagaagta taaattgtgg
      841 taagaagtca aacgaatccc aaatgtttca tcacatttga tcaaatggcg gatataggca
      901 cgtgtatagt ttttacatgt ataacaatca catttctcat caattggacg gaaatcatgg
      961 gcatatttcg catttttcac tactagacgg ccttgagaag tcatacatgt gccatttcga
     1021 gcgattcgag taggcaagac acagtcaaac atgtctactc cacggataac accatcgatc
     1081 aatgaatcag cagcaccgac acccatcaag taacgtggtt tgttttcggg gatcaacggt
     1141 gtagtgaatt ctaatacacg attcatttct gatttaggtt ccccgacaga taacccgccg
     1201 attgagtaac cagggaaatc catcccgact agatctttcg cgctttgacg acgaagatct
     1261 tcaaaccctg ctccttggat gattccaaat aatccttgac ggtctggatt agcatgagct
     1321 ttcaatcctc gttctgccca tcgagacgta cgttctactg attttttcac gtagtcgtaa
     1381 ctttcatcga atggtggaca ttcgtcaaaa ctcatcatga tatcagagcc caatttattt
     1441 tggatgttga ttgctttttc tggtgataaa aacatacgcg aaccgttcaa gtgatttttg
     1501 aaatgcacgc cttcttcttc gatattccgc atatcactta atgaaaaaac ttgaaagccg
     1561 cctgaatctg ttaaaatcgg ttgatcccag ttcataaatt tatgcagtcc gccagcttct
     1621 tctactaagt cttcaccagg gcgtagccat aaatgatatg tattacttaa aataacgcca
     1681 gcacccattt ctttcaactc ttctggggac attgttttta ctgtagctaa tgttcctact
     1741 ggcatgaaca ttggtgtggg gaaagttccg tgtggcgtga tcaattcccc taaacgcgcg
     1801 ccagtatgtt tttctttctt gattaaacga tagcgaatgg caggttgcgt cattcatttc
     1861 cctactttct atataaaata tcttgacaac tcccaccatc ataatcgttt caagtgcttt
     1921 ttgcaagttc cctcggcttc cttctacttt atttaatatt ctttttttta tccttttttt
     1981 atgaataaca aaaaaaagaa aaacattatg acagaaatat tttttgatac atggtatatt
     2041 ggtgttatta aagaaaagga gggaaaaatc atgcaatctt ctgcagaatt aaaacgccaa
     2101 gcaaaagaga gtctaaaagg acgttgggga caagctgttt tactaaatct aatccctaca
     2161 ttgattggta ttgctactat tcttcttatt atgattccta ttatcttgtt tgccaccacc
     2221 atatatgatg gatcagcgag cgtctcagaa gcgacaactt caggaggcgg tagttcagga
     2281 ggcggtggcg gaattatttc cagcatcatt agcgctttgt tcatgagcgg tatttcttgg
     2341 acttatcttg atattttacg tggacaaaaa gatcagatcg aaccattcaa agatgcattt
     2401 cgtggattca aaggtttgtt tatcggcgga gtgatcagct tagctgtgct gatctcgatc
     2461 tttacaacat tatggggttt gctattagtt attcctggaa tcattaaaag ctattcttat
     2521 tctcaaagct attttattta ctacgatatc gtccaagaaa caggacaaac acctaaagta
     2581 ctggatacga tcactgcaag tcgaaaattg atggatggtt ataaaggaca acttttctgg
     2641 ttagatcttt catttatcgg ctggcatatc ttagcattag caactgctgg tattggttat
     2701 ctatggctca gcccatacat ttctgcaaca aaagcagcat tttatgaaca attgccaaaa
     2761 gatatttaaa agagagtgtg acttgtgacc gagggggtgg caggagaaca ccgtttatcg
     2821 agaggcagta aaagaaacat tcagcccatt agcaaaaagg aggtgtgaca acattcatgt
     2881 cacacctcct ttttgtatag ttccagtaaa aagaaaatca acttagaaac agttaatcat
     2941 tcctcttctt ctgcttccgg taatcgatgg aacttgatca gttcattcac cgcgattggt
     3001 acgatagata aaatccctac caagaaccag tgattcaaac tgatgtgtac taggccgaat
     3061 agttcttgcg taaatggcag caaagcaaca gccgttgtaa tagctaatgc cagtaacgcc
     3121 atttctacca aagctttatt ggtcgctaaa ttgactttga agatcgattg gctgctgcgt
     3181 acattgaaca catgcagaat cgatgaataa gctaagatca agaatgcaac agtttgaccg
     3241 acatgctgac ttgcgtctac ccatgggctg atagtatcga cataactacc taaataataa
     3301 ccaaacaagg ttacgatggt gaagacccct gcatttatgg caattttttg caataaacct
     3361 cgagcaaaga tcccttcatt tttcggaatg ggtggctgat ccatgatgcc gtgttccgct
     3421 ggctctcttc ctaaagcaaa tcctggaagt ccatcagcaa ctacattgac aaacagtagt
     3481 tgcacagctg taaatggtgc tccccatcct aacagcatcg caatcaaaac aataaagatc
     3541 tccgaaatat tacagcttag caaaaagttg atagctttac gaatattctg ataaaccgaa
     3601 cgtccttgac tgacagcgtc tacgatcgtc gcaaagttgt catccgtcaa aatcatatct
     3661 gcggcacttt gcgccacatc tgttcctgtg atccccatgg cacacccaac gttacttgct
     3721 ttcaacgctg gtgcatcatt cacaccatcc ccagtcatcg ctacgactgc acccgtacgt
     3781 tgccaagatt tgacgatccg gatcttgtct tctggcgtta cccgagcata gacagagaga
     3841 gctttgacac gcgcatctaa ttcttcgtct gataattgat gcagttctga tcctgaaagt
     3901 gcttctgacg gatcattcaa gatacctaat tctttcgcta tggcactggc agtcaccaca
     3961 tgatctccag tgatcatgac tgttttgatg ccagcctttt tcgctcgctt gattgctcct
     4021 ttactttctg gacgtggtgg atcgatcatt ccgatcagac caatcaaacg taagtctttt
     4081 tccagtgctt cagaagaaat atcttgcggt tcttcgtcat aagtagcata accaacagca
     4141 atcactctca gtgcccgttt accaaagcga tcattaacga tcgccgcttg ttctacatcg
     4201 cctgaactaa agcgcggagc aagaacatcg aatgctcctt tggtgattga gatgtatttt
     4261 tcgcccattt ggtgaactgt tgtcatcatt tttcgttcag aatcaaaagg aagctcgttt
     4321 actcgtggat atttttcttc taattctgcc tttgtatgat aattctcttc aacagccctt
     4381 acgatggcta cttctgttgg attacctttg ataacaaggt cgtcatcttc ttgctcgacc
     4441 gtcacatctg tacatagtgc agccattttc aatatttcca tcgcgctgtt cgtcattgaa
     4501 tcttcgatat tcgttacttc atcttctttg ctccataccc ggcggacgcg catttcattt
     4561 tgtgtcagcg ttcctgtttt gtctgagcag ataacattag cggttcctag cgtttcaact
     4621 gctggcaact gtcggatgat cgcatgtttc ctcgccatct tctgcacacc gtatgcaagt
     4681 gtcaacgtaa caataacagt caaggtttcc ggtacagctg caacagccaa ggaaatcgaa
     4741 gtcatgaaca tctcaaggag tggttctcct tgcagttcac caatcacaaa tactaaagcc
     4801 gcagccgcta aagcgatcat actgattcgt ttgcctagct gattcaatcg gacctgtaaa
     4861 ggtgtttttt gcatagtatt ttcgttcaaa agatccgcga tcttacccat ttctgtagcc
     4921 atgccaatgg cagtgacgat cgctttccca cgacctgcag caaccgtaca tcctttaaat
     4981 accatattaa gttggtcacc taatgactca tcttcttcag caataaatgc tgcatctttt
     5041 tcaactgctt cactttctcc agtcaacgcc gattcttcta cacgtaattg tgatgcttcg
     5101 atcaaacggg catcggcaga aatagcatca ccggactcta aaacaagaat atcacccact
     5161 accagttgct ctgactcaac agtggtcact ttgccgtctc gtatgacagt tgccgtttgt
     5221 ttattcatat cttgcaagga ttccagtgct ttttctgcgt ttccttcttg gacaatcgcc
     5281 aataccgagt tgatcacaac gatagcgatg atcagcagtc cttcaaataa atccccatgt
     5341 tctgtagcaa atgcggtata aaaggaaatc accgctgcta caagaaggat gatcgttgta
     5401 aaatcggaca agcttcgaaa gaattttttg atcatcgatt ctttcggcgc ctcatcgaat
     5461 ttattcaatc cctgcttttt tcgccgttca tccacttctt gtgaagacaa cccagtatct
     5521 ggatctgctt ctgttacctt ctttacttct tctattgtct gcttgtattc ttccatccta
     5581 ttcctccttt aaaatcttcg tacaaaaaaa ggtacttatt ttactataac atcctttttt
     5641 tatttacttc aaataaaggg cttatcgatt atttgttaag aaagcgtaaa atttaaagtg
     5701 cttatatttg caaacgctac caaaggatgc tattatatag atgtaaggaa ggaggatacc
     5761 atcaaataga cagaagaatt tctcgataga acagtgtaag caaacaaagt tgttagaagt
     5821 acaggtttca cccagacatg cgtgtagagt acaaacctat aaatagtgaa tgcgtatgga
     5881 tgtttggatc acgaagtatc cgaaacaaga agtaatgcga tacgtaacaa ttgaataacg
     5941 taaacgtctg aaaattgaat aggaacaaga gatgtttcaa atgttgaaga tgtaacgaaa
     6001 acaaaatttt tgtttaacat gaagaaattc aacaccgtgc tttgcatgta tgatggaaag
     6061 ttacaatcat ttctgtttat ttgaatcctt ccgaggtaca agaaagccga gcttatcgag
     6121 agctcggctt tttctttgtc ataaaatctt tgtaaagctt ttttgttatt caaaactagc
     6181 ggcttttcat aaccttattt cacgaacatc gcatctccaa aactaaagaa acgatagcgc
     6241 tcttcaatcg catgctggta agcagataaa gtcaattctc ttcctgcaaa ggcactaact
     6301 aacatgacta aagtcgattt tggcaaatgg aaattagttg agaaagcttc taccactttg
     6361 aattcatagc caggcgtaat gaaaatatca gtccatccgc tgtctgcttt gatttctcca
     6421 ttaaatttcg ttccaatcgt ttctaatgtt cgaattgaag ttgttccgac agccacgatt
     6481 ttcccgccat ttttacgtac ttggttcaat cgctcagccg cttcttccgt caaacggtag
     6541 aattcactat gcatatggtg ttcttcaatt ttatctacgc tgacaggtcg gaatgtacct
     6601 aaccctacat gtagtgtcag atagactaat tcaacaccct ttgcttcgat ctcatcaagt
     6661 aattctttcg taaaatgcaa tccagctgtt ggcgctgcag cagaaccatt ttctttcgca
     6721 tagacagttt gatagcgatc tgggtcttcc agacgttctt tgatatatgg aggcaatggc
     6781 atttctccca atgattcaag aatctctaga aaaattcctt catactcaaa gtcaatgatc
     6841 cgtccgccgt gttccaattc ttctttcact actgcttgca atctgccatc accaaaagag
     6901 attcttgtcc caactttggc acgtttcgca ggtttgatca atgtctccca cgtatctcct
     6961 tgtgtattcg ttagaagaag gacttctaaa tgcgcaccag tatctggttt ttctccatat
     7021 aaacgagcag gcagaactcg tgtattattc ataactagcg catcaccagg ctgtaactct
     7081 tcaatgattt catggaaatg tttatcttcc atctctccag ttttccgatc caaaacaagt
     7141 aaacgagaat gatcgcggtc ttttaatggt gtttgtgcga ttaattcttc cggcaagtca
     7201 aaatcaaaat cttctgttgt taacatatcg tcactcactt tctatcatgt gcttcatcat
     7261 tctatcatgt ttttcgttct gttggcaaaa tcagcacttt ttcacttcta ttttttgttt
     7321 tcctaaataa aaaaactacc ctatttgtat tttttcattg ttttaggcat actagtaata
     7381 gatagttctt cattgaaaat agaaaaagag gtggcaccat gattgaattt caaaatgtgt
     7441 caaaaattta taaaggtgga aaagtcgctg tagaggacgt caatctttca tttgaaaagg
     7501 gtgagttcat ttgttttatc ggtacgagcg gaagcggaaa aacgacgacg atgcggatga
     7561 tcaaccgaat gactgatcca acacaaggga aaattttgat tgatggaaaa tccatccaag
     7621 aaattgatcc agtggaattg cgacgtcaga tcggctatgt tatccaaagt attggattga
     7681 tgcctcacat gacgatccgt gaaaatatca ctcttgtcca aaaattgttg aaagtcagtc
     7741 aagaagaacg gaataaaaca gctgaaaaaa tgattgatct tgtggaattg ccccgtgaga
     7801 tgttagatcg ctacccacat gaattatctg gtggtcagca gcagcgtatt ggagtcgttc
     7861 gtgcccttgc agcgaatcaa gatatcatct tgatggacga gccattcggt gcgcttgatc
     7921 caatcactcg tgattccttg caagaccttg tcaaagattt acaagagcgt ttgggaaaaa
     7981 cgatcgtatt tgtaacccat gacatggatg aagcgattaa actagcaagc cgtatcgcca
     8041 tcatgagtga aggccgtgtg atccaatttg atacaccaga aaatatttta cgtcatcctg
     8101 caaacgattt cgtagaagag ttgattggag aagatcgcct actacaagcc aagccaaatg
     8161 cgacaagagt cggcgaagtt atgctaaata cagcaatcac gattacaccg gaaaaatcat
     8221 tacaagaagc aatcaaactg atgagggaaa aacgtgtaga tacgttgtta gttaccgata
     8281 attcgaatgt attaaaagga tttatcgatg tagaaacgtt gaataaaaaa cgaggaaaag
     8341 tatccagtgt cggcgacatt ttgaataaac atgtttttta cgtaaaagaa tccgcctatc
     8401 ttcgtgatac cttgcaacgc attttaaaac gcgggctgaa atacgtgccg gtcgttgatg
     8461 aacagaacaa agtcgtaggt atcctgactc gtgcatcatt agtggatatc gtctacgatg
     8521 tgatttgggg agaagatccc gcaactactg atgttgcaga gtcagctact cctgaagaat
     8581 ctaaggaggt ctgacctatg cagcagtttt ttcatcagca cggttcagaa attctctcaa
     8641 aaggttttga acatctttat atttcaggac ttgctttgat cattggtatc atcattgctg
     8701 taccgcttgg tgtcttactg acacgttttc caaaagctgc cacgattgtg attggcttaa
     8761 ccagtgcctt gcagacaatc ccttctcttg ctttattggc attgatgatc ccgctatttg
     8821 gcgtaggtaa attacctgcg attatcgcac tctttattta ttcattattg ccgattttac
     8881 gtaatacgta cattgggatg aaaaatgtcg attggaatta tcgggatgtg gcaaaaggca
     8941 tggggatgac tgagttccaa tccatttttt ccgttgaatt gcccattgct atgccaacga
     9001 tcatggctgg tattcgttta gctgccgttt acgtgattgc atgggctaca cttgcctctt
     9061 atatcggcgc tggtggttta ggcgatttga tttttagtgg actgaataat taccaaccag
     9121 atctcatttt ggcaggtacg atccctgtaa caattttagc actattggct gattggcttc
     9181 taggtctctt ggaacaccgg ctgacaccga tcgcacttag agaggagaat gaagaatgaa
     9241 acgtatacta aaaatgacca tggttctttt ctctcttctt atacttgcga gttgttcctt
     9301 ccctggtctt gccagcaata cagatgaaga caccatttcg atcacaggtg gaatcacttc
     9361 agaagcacag atcttagcaa gtctagttgc cggaatggtt gaacattata cagataaaaa
     9421 taccgcaatc atcaacaatc tggctactac aactatcaac catcaagcga tgatgaacgg
     9481 agatgcttca atttcagcgg ctcgctacac aggaacagat ttaacgacta cattgaacct
     9541 tcctcctgaa aaagatccga aaaaagcgtt tgctaccgtg aaagatgaat ttgagaaaag
     9601 atacggtcaa acgtggttcc cgtcctatag ttttgaaaat acgtatgtct ttcttgttcg
     9661 aaaagacacc gcacaaaaat accatctatc taaagtcagc gatctgaaaa atgtagcgga
     9721 tgaattagta gctggtgtag atacttcttg gatcaatcgt aaaggagacg gttatgacgg
     9781 cttccaagaa acttatggct tctccttcaa ttcgatatta cctatgcaga ttggacttgt
     9841 ttatgacgca gtcgaagcag gaaaaatgga tattgttctt ggctactcta cggatggacg
     9901 gatcgccagt tatgatctag tcatgttgga agatgatcgt caattcttcc caccatatga
     9961 tgctgctcca gttgtagaca acaagatatt agctgatacg cctcatttaa aagaagcctt
    10021 aaataagtta ggtcatacca tcagtacgga aaaaatgcag gaattgaact acgaagcaga
    10081 taataatcta gtggaaccat cagtagtagc acaacgtttc ttacaagaaa accactattt
    10141 tgaagggaag tgaagcaatt ggataaaatg aatgtctttc aacaattttt ctattatttc
    10201 caggaaaatg gctcctatat ctttgcacaa tttatccgtc acttcctgat ttctatctat
    10261 ggtgtcctct ttgcagcagt tgtcgggatt ccggttggga tcatgatttc taggagacgt
    10321 aaattagcaa actgggtgat tcgtttggcg aatatcatcc agacgatccc ttctctagcc
    10381 atgatctcca ttttaatcat tggattaggc ctgggagtca atgtcgtgat tgttactgta
    10441 tttttgtatt ccctcttacc gatcatcaag aatacgtata ccgggatgat ccaagtagac
    10501 aaaaacatcc ttgatgtcgg caaaggcatg gggatgactg ctagacaacg actattcatg
    10561 gtagagttac cactctctgt ttctgttatc atggcaggca taagaaacgc gttagttgtt
    10621 gccatcggga taaccgcgat cggtgcattc gtcggtgctg gtggtcttgg agatatcatt
    10681 atccgaggaa ccaatgcaac agagggcaca tcgattattt tagcaggtgc attgccaact
    10741 gcattaatgg cgatcatcac ggactggctt ttaggtattt tggaaagacg gctagatcca
    10801 gccagccgta catcacgctg atgttattgt aaaaaacaaa agcttctgcg accatttgtt
    10861 ggtcgcaaag gcttttttag ttctgtcatt tttaaggcaa taatccactc tgattcttcc
    10921 ctccttatgg gcaaagagtt gcttttctta gctatttatc cattccgtat actccaactt
    10981 catactgcca acgggttcat atctgcatcc accgactaag tttttttctt atccgctaag
    11041 gcattttgcc aatccatttt cccatcactc gtcatggaat tcataccaca tgatctctaa
    11101 atactcgtct gcttgttctt tgaattgggc atcaattctt acatcctttg gcgtggcttt
    11161 catttaagcc ttttccatca gtcctcgatt gttcgatgcc ttcagctgca tcatcaaaac
    11221 tggtgactgc cgtatcggta acttcgatca tcttttatac aatcgggaac ttgccatcct
    11281 catccttggc taaaaacata tctagccatt ccagcttctc gccatgggag gtttgcggtt
    11341 cctaaaaccg gtacacgatt cttatggact gcatccgtcg caactagact tggcgatacg
    11401 atgatccttc accagaagag cacccccgta aaccaattga tcgatatatt gccaataaga
    11461 aaagacattg gcgtaaaaga tatcgatccc tcgtgatgca ttgccgttga tactgctatt
    11521 catggccgaa agcgccacga ctttaacttc ttccgtttga gtgacattca tgttcggcaa
    11581 tgtcgctttg gatacccgct ctgctaaagg aacgacactg gtattgtatt tcgtatctgt
    11641 atcttttcca aaagaccatg ccaacgaatc ctctgggaac caataagacg actccggttg
    11701 attgtccatt gcttttttca acatactttt ggctgtttct cgataaacga catttccaat
    11761 tgtcttcgcc tcactttggc gataatcgat ggtcgttgcc actgccaaca ctaacaacga
    11821 tgctccaatt ttactgattt tccttgtcac acttcttcct ccttgtttta cataaacccg
    11881 tcaacattcc attcctaaac tcaaacaatg acccttcaaa ggtggagata taggaacaaa
    11941 ttaaagaaat catgcaataa atccaagaag gttggtttta gaacaaaaaa agagtctcct
    12001 ttttggggag actctttgtt aactatctaa aaaggttggc tcttctcact tcactcataa
    12061 tgtgtccagg cagaataaat ttctacaact ttattttcct catcaacttt gtaaaccact
    12121 cgatgctgtc tattcagcct gcgtgagtat ctgccttcat gtgtaggtct cagcttttca
    12181 aaagactgcg tgggcatata tggatcttcc ttcaatgtct gaatcacttc ttcaaattgt
    12241 ttctttaaat tcgtttgctt gatttttctt aaatccactt tcgcagagtt tttgattgcg
    12301 actgtatagt tactcatcaa agagcttccc aatcaatatc atcgatattc gtaaagctac
    12361 tgtcgtcttt ttcgcggtct cgtactttat ctaaagtacc tgtttgctca agaaaaaggg
    12421 tttcttgaat cgctctccag tcttccagcc caatcaatac agcattgttt ccactacgat
    12481 cactaataat ctcgatttca gtatgatttt catttacctc tttcaataac tgataaaaat
    12541 ttttacgagc agtgctagga ttcatcgttg tattagccat tgtctctcct cctttttctg
    12601 atataattat agcgtacttg attaagtacg tcaaaaaaat acaataaagc acttaaattc
    12661 agcaaacttt tcctcttttt tacatagaga aaaagacccc tttttaggga ggatctttgt
    12721 ccacgctcac ttcttactcc tttacgacta gcggtagcca aacgtaaagt aattggtaac
    12781 aacgtatcat ctttagcaaa ggtcgaatcc aaagcctgtg agtctatgca acaacaccaa
    12841 tacacgaagg ttcctcgttg cagtgtgact tgattgatat cttgaataac ctgaggatac
    12901 gcacgaaaga tttgtaacgg ttattcaaaa ctgattttgg ccgtcaagtc ttctactgcc
    12961 tcaaaaatga ttgcctcttt cgtctcctgt cattcatgtt cactatcgat ttgatacttg
    13021 cttagaccgt tgggtttgtg aatcaccaat taaaaatggc actgagttat ggtaacaaaa
    13081 aatggtcact tgacgaggct gagattccta cttatgaaga cacatggcac ttaagagtcg
    13141 aggtaaagga agaagctgaa aatataggag gctgtacatt atttggcaaa ggattcggaa
    13201 attatgatca agacatcaaa atcaaaatgt tttacagcta aataggaaga ataataacat
    13261 ttcatacgct gttcccatct ttaatggctt atagagggca cctttagctg agaataagac
    13321 aaaacagacc ctctagcttc aattaagagc tagagggtct gtttttgatt ttaaaacttg
    13381 gtttatttct tcaaatggta gctataggtg ctgacaacga tattttcacg ccaagacaat
    13441 cgcaatctta accgcaaact tgtttggtta tggagaatat tctgccagcg tctgtttggt
    13501 acaaattgag gaatcaacac agtagttgta tagttttgtt tcgctgcatt tttacttacg
    13561 atatctacat aacggataat tggattttca atcgagcgat aagaagagtg gacgattgaa
    13621 aaacggacat ctgggaaatg ctttttaaat tctgcttcga tttctttttc tttttcgaca
    13681 tcttcgtcta gcgaaacatg catcgcaacg acataatcac cgatcgaacg tgcgtaattc
    13741 aacgcaccga tatttacccg tgtcacattc ccgactaata caagaacggt attcccgtca
    13801 taatcatgta gttttgcatc attttctaaa cgaagctgtt ctgctacttt tttataatga
    13861 tcatggatct tataaaaaat gaacataacg attggcataa tgatgaagaa aggccaaatg
    13921 tcccctaacc gatagacaaa caaaatcgca atgattgcat atgagatgaa cgcacccgta
    13981 atattggcaa tcgatttgct taaccatctt tttccttctt ttttccattt gacgaccatc
    14041 cctgtttgag ataaagcaaa tggaatgaac acgccgatag agtataacgg aatcaagcgt
    14101 tctgttgagc catggaaaat aaacagcaat acgatcgaac ctagcgctag tgtgataatc
    14161 ccattggaat agcctagtcg atccccacga tcttgataca tgtgaggcat gaatttatct
    14221 tttgccagat tgtatgcaag aactggaaaa gcagaaaaac cggtgttagc agcaactgct
    14281 aaaatcagcg ctgtagcaaa ttgaaggacg taatacaaaa taccatgtcc aaagacagct
    14341 ttaccaattt gagacaaaac agtgacttct ttttcaggaa cgatcccata ccaatagttg
    14401 ataaatgtga tccccacgaa gaagaaacct aagatcaatg ccatcatcgt caatgtagca
    14461 gcggcatttt ttgctcttgg cttcttaaag aatggtacag cattactaat cgcctccacc
    14521 ccagttaaag aggaagaacc agaagaaaat gctcgtaaaa tcaaagcgat agaaacaccc
    14581 ggcacagccg ctcctggaag tgcagtagct tgcaatggca cagcacctgt cacgatatta
    14641 tataaaccaa caacgatcag taacgagatc acaaggataa atgtgtacac cggaaataat
    14701 aggaacgatg cagattccct cagcccacga agattcagca tcatcagtag caaaacgatt
    14761 gtcacagaaa tagcgacttg atgaccgtag agtgctggca ccgctgatgt gatcgcttca
    14821 gcacctgctg agacagatac tgctactgtc aacatgtagt cgatcaataa agagccaccg
    14881 gaaatcaagc cagcattctt tccaagattc tcactgctga cgacatatgc tcccccgcca
    14941 tgaggataag catggatgat ttgacgatat gatagcgtta atgaaatcaa taaaataata
    15001 acaaaagcgg caatgggtag cgaataccag atggctgcag cagacaaagc gactaaaacg
    15061 acaacgattt gttccgtacc atatgcaata gatgataacg catcagacga caataatgcc
    15121 aaagcagcaa atcgggttaa tttgtgctca tcattttcag cagattttaa aggcttacct
    15181 actaacaacc tttttaagta atccaatttc taaactcctt tacacacaat tttacttcaa
    15241 gttttatagt cgaaaattt
//
LOCUS       DAZBTF010000065        14961 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000065 DAZBTF010000000
VERSION     DAZBTF010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.065"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..2377
                     /locus_tag="ACHGNS_002161"
     CDS             5..2377
                     /locus_tag="ACHGNS_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFN6938084.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2438..3898
                     /locus_tag="ACHGNS_002162"
     CDS             2438..3898
                     /locus_tag="ACHGNS_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFN6938085.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3909..6113
                     /gene="topB"
                     /locus_tag="ACHGNS_002163"
     CDS             3909..6113
                     /gene="topB"
                     /locus_tag="ACHGNS_002163"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFN6938086.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6129..6278
                     /locus_tag="ACHGNS_002164"
     CDS             6129..6278
                     /locus_tag="ACHGNS_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938087.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6353..6946
                     /locus_tag="ACHGNS_002165"
     CDS             6353..6946
                     /locus_tag="ACHGNS_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFN6938088.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6959..7858
                     /locus_tag="ACHGNS_002166"
     CDS             6959..7858
                     /locus_tag="ACHGNS_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFN6938089.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7861..8358
                     /gene="ssb"
                     /locus_tag="ACHGNS_002167"
     CDS             7861..8358
                     /gene="ssb"
                     /locus_tag="ACHGNS_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFN6938090.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8724..8984
                     /locus_tag="ACHGNS_002168"
     CDS             8724..8984
                     /locus_tag="ACHGNS_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938091.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9429..9635
                     /locus_tag="ACHGNS_002169"
     CDS             9429..9635
                     /locus_tag="ACHGNS_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFN6938092.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9656..9886
                     /locus_tag="ACHGNS_002170"
     CDS             9656..9886
                     /locus_tag="ACHGNS_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938093.1"
                     /translation="MTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLVEDAPTML
                     FEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            9902..10339
                     /locus_tag="ACHGNS_002171"
     CDS             9902..10339
                     /locus_tag="ACHGNS_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938094.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10332..11039
                     /locus_tag="ACHGNS_002172"
     CDS             10332..11039
                     /locus_tag="ACHGNS_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938095.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11203..11403
                     /locus_tag="ACHGNS_002173"
     CDS             11203..11403
                     /locus_tag="ACHGNS_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938096.1"
                     /translation="MNKIIPLLFVLCIGEILMRHGAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKLIAAIFFIGLFR"
     gene            11419..11598
                     /locus_tag="ACHGNS_002174"
     CDS             11419..11598
                     /locus_tag="ACHGNS_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938097.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            11923..12186
                     /locus_tag="ACHGNS_002175"
     CDS             11923..12186
                     /locus_tag="ACHGNS_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFN6938098.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            12180..12530
                     /locus_tag="ACHGNS_002176"
     CDS             12180..12530
                     /locus_tag="ACHGNS_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFN6938099.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(12554..13168)
                     /locus_tag="ACHGNS_002177"
     CDS             complement(12554..13168)
                     /locus_tag="ACHGNS_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN6938100.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            13482..13904
                     /locus_tag="ACHGNS_002178"
     CDS             13482..13904
                     /locus_tag="ACHGNS_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938101.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            13914..14117
                     /locus_tag="ACHGNS_002179"
     CDS             13914..14117
                     /locus_tag="ACHGNS_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938102.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            14328..14912
                     /locus_tag="ACHGNS_002180"
     CDS             14328..14912
                     /locus_tag="ACHGNS_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN6938103.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         5511 a         2429 c         3036 g         3985 t
ORIGIN      
        1 gaacatggca gaagaaaaag aaccactaga caatagtcgt ttgggaaaat ccaaaagaaa
       61 actagtacga ttgcaaaatg aattaaacga acaaattgaa aagatgtttg aacaccaaaa
      121 aatgacgaac ggtcaaccaa tgaacgacaa gaggaatggc cgttcatggt ttaaccagca
      181 agaacgaatc gaaaataaag tccaaagtct gagagaagag atcaagcaac aagaagaata
      241 cgtggaaaag cttgaaagac aagaagagct aaaggaaatg ggctataaca agtacggcgg
      301 actggatatg acgatcgata atattccaag aatcaaagaa gaaatcgaac gctttgaaaa
      361 aggtgaatcg gctttttcag ccgccacgat ccgaaaatac caacggaagt tggaaacatt
      421 agaacagctg aaagaaaaat cagaaaaagg aaaagaaaat atcctccctg aagtacaagc
      481 gatcattgac agcggacgag tgacgcaatg gaaaaaaaat ccaaccattt attttttgaa
      541 aggctatcga aaagttgctt tagaactgaa tgaaaacggg ggatttgagg aatcaaaaaa
      601 ataccaagcc acgtcggaag aggaaaaagc agctgttcgt gaattacttg aagaggctag
      661 accaactgat tctatgaagg agaaagaaaa cgatcatttc tatcgagtag aattcaacga
      721 aaatgatcca gaaaaaggat tgaaaagtta tggtggggag atcgtaacat tcgaattgat
      781 cgatcaactc aaaacattgg ataaccaata cgccgcagaa gcaggttatt ataaattcta
      841 ttttgaggaa gtgaagaacg gagaagtagt cgatcagatc cgaatggatc taggggatgg
      901 cgtcaacagt aatcaaccaa tctatttaga gttagcaaaa ttgtgcgaac aagtacctga
      961 gaaggaacaa aaacaatcgg tcaaaaatga acctgtttcg aaagaacaaa tgcagtcgaa
     1021 agaaacaaaa acaaaacaaa agggctatca aaaacgaacg gcagaagaga ttaaacaaga
     1081 agttaaatcc ctttcgcaac aggcactgga tgcggtgaaa aagcataccc attctcctga
     1141 agacgtcaaa gaacttttga actttatgtc tcgttttcca gaacgatcct ttagaaatca
     1201 attattgatt gaggaacaat ttccaggtgc aacggcttgt ttaggtcgtg ccgcgttgaa
     1261 aaaagaagga atctatatca aaaaagggga acaagggaac aagatctttg tcagaaaaac
     1321 ggtcaaaggg ttttatgaga aaggacgtgg ctttgttcga gaaacagaag ctacaccaga
     1381 agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc aaaaaacctt actatacgat
     1441 tgaaaaagtg tttgatatca cacaaacaca gttaaaacct gaagactatc cgaaaatctt
     1501 tccgaaccgt gtctttgatt ttcaattgga taaaaaagga cagtcagaac ttcaagctgg
     1561 gattcaagcg gtagccaaca atattggaat acaaattcga gatatgacag aaagtgaagt
     1621 ctatcaaaga gaattaggac aagcacgtgg ggcgtatgtt cgtaacgaat ttactggcga
     1681 agaagaaatc gtcatgaata ctcgaaatac tcctacgcag catttagcta caagtattca
     1741 tgaattagcc catgcaagaa tgcacaaatt ttcagaatta gataccgcaa ctaaagaatt
     1801 gcaggcagaa atgacttctt atattgtgtg caaacatttt ggaatggaca cgtcggagaa
     1861 agctattcct tatattgcag catggacgaa gaatggtcag acgttggatg ataaggaagc
     1921 tgcggaacgt gggaaaatca tgaatgatgt cagccgtgtc gcaaatgaat tcattcaaac
     1981 catttctaat gaaatcaatc aatacagaga aagagaacca gagatccaac cagcagaagt
     2041 gaagcaggag cataaaaata ctttagaaac gccagagaat aagaatattg tgcccgttgg
     2101 ttcactttgg atcgacaaga aagacgggaa agtaatggaa gaagatacgg gtcgaacgtt
     2161 acatttgaag gatgcggcgt ttattgaaga tccgcagcat aatcaagtaa ttttaaaagg
     2221 aaaaagttat ggaaaagaga tccaaacggc gttaccttca gaagaatttg cgctaggaaa
     2281 tccattccga ttttcacttg agacagtgtc agaagtggca aaacgaaaaa atgaaatcta
     2341 ttatgaacaa caattgtcac aaaaagaacg tgtataaaac aagtaaaaaa gactatctga
     2401 aaaacggata gtcttttttc ttttgggagg ttttaaaatg accgagaaaa aaatgagtct
     2461 tattgatcga tgtaaacaga tcgatatagt ggattttgct cgaaacaatg gaatggctgt
     2521 agtcaataaa ggacgagatt atcgcttaga agaccatgat tcatttgttt ttgatcgaag
     2581 aaaacaacgt ttttattgga acagtcaaaa tattagtggc gacattattg aattagctaa
     2641 actttttttc atagacaaag agatccaaga ccctaagcaa caatttaaag cagccactga
     2701 ttttattcta aagaatgaag acaaaacaga acgagtggaa aatcttcatt ttgaaacaga
     2761 aaagtacaaa gatcaccccg ttgattatca accactcaca gaaaaaggaa ggaattatct
     2821 gaaggaagaa cgaaaactac cagagtggct gattgattat gcagaaaaag aagggttgat
     2881 tgccgaacta aagccaaaat atgaacggca aaatttctta gttcgagacg atcgtttaga
     2941 tcatgcggtc gcctttttat ggaaagatcc acagacaaaa gagacagtag gggcgagtta
     3001 tcaaggaacg ttcattgatt atgaacgttt tggtgagaga ggcacatata agcatataga
     3061 taaaaattcg acagcgaatc atggctttaa cctaaagatc ggtgacccta agcagataaa
     3121 attttttgag agtagtattg atttacttag ctatgctgca ctgaatcgtg atcagttaaa
     3181 cgatacgtgg ttagtctcta tggaagggtt aaaacaccat gtgatcagtc attattttgg
     3241 tgaggcagta tctgaattga gaaagaaaca agcctttcct caatccatcg agatttgtgt
     3301 ggataatgac cgtgcaggac atattttcta cgaaaaagaa caattaatgg gcgcagtcga
     3361 tccatttacg aatcagaaag tacgttgtga acgaggaatc gctaatgatt ggcaagtgcc
     3421 aaaagaatac aaaatcattt atgaagaagt cgcaaaagaa gaaaaggtca caccagaagc
     3481 tattatggcc atacacaaga cagaaaacaa tctgcaatta acgaaccaac tggtttccgc
     3541 tcacaaagta aatgcgtttt ttggccagca gctttctgtt aacgattcaa tagaagccat
     3601 taatttgaaa gatatttgta gagaagtcgc aaaagaatta aaagtttgtg aacgcgtgga
     3661 tggtacgtac gattttgatc gtttttatca agaaaaaggg gatattaatg cgcagattct
     3721 tttctcttat aaagcagaac aatactacaa aggctataaa aatcatgaac atgaatttgt
     3781 acctgaagta aaaaaggact ggaacgatca attgaagcat gagatccagc aacaagaaat
     3841 cagaaagcaa aaacgtgcca tgttgtttca acaaggcaga caacaagaaa gggagtgaaa
     3901 tcgtcatgat gaaaaccgtt attttagcag aaaagccgtc acaagcgaaa gcttatgccg
     3961 atagcttctc taaagccact cgaaaagacg ggtattttga aatccaagat cgattgtttc
     4021 ctggtgaaac tgtgatcacg tatggttttg gtcatttagt cgaattagat tcgcccgata
     4081 tgtacgatga aaattggaaa caatggtcat tagaacattt gcctattttc cctacacatt
     4141 atcactatca tgttcctaaa gataagaaaa agcaattcaa ggtggtgaaa cagcaacttc
     4201 aatcggccga caccattatt attgcgacag atagcgatcg tgaaggggaa ctgatcgctt
     4261 ggacgatcat tcaacaagta ggcgccgatc aaggaaaaac ctttaaaaga ctttggatca
     4321 actccttaga aaaagaagcc atttatcagg gattccagca gttacgagac gcaaaagaga
     4381 cctatccaaa atttgaagaa gcacaagcac gtcagatcgc cgattggtta attgggatga
     4441 acggtagccc actgtatagt ttactcttgc agcaaaaagg cataccagga agcttttcct
     4501 taggcagagt ccaaacccca acgctttaca tgatttatca attgcaggaa aagattagaa
     4561 atttcaagaa agaaccttat tttgaaggaa aatccgaagt aatcgctcaa aatggcgcat
     4621 ttgatgcgaa gcttgatccg aatgaaaccc aagcgacgca agaagcattt gaagattatt
     4681 taaaagaaaa gggggttcaa ttgggaaaac agccaggcat gattcgccaa gttgaaaccg
     4741 agaaaaaaag cgcggcaagt ccacgtcttt tttctctatc cagccttcag tctaaaatga
     4801 atcagctcat gaaagctagt gccaaagaca ctttggaagc catgcaaggc ttgtatgaag
     4861 ggaaatatct aagctatccg cgaacggata ccccttacat tacggaaggg gaatatgcgt
     4921 atctgttgga tcacttagac gaatacaagc attttttgag ggctgaagaa atttcgactc
     4981 ctattcatac accgaatagt aggtacgtga acaacaagaa agtccaggaa cattacgcaa
     5041 tcattcctac aaaaacagtt atgacagaag cgacgtatga aagtctgaca cctttacaac
     5101 aagcgattta cgagcaagtt ttaaaaacga cagtggcgat gtttgcggaa aaatatacct
     5161 atgaagaaac aacgatcttg acgcaagtcc agcagcttca gttaaaggca atcggaaaag
     5221 taccgataga tctaggttgg aagaagttat ttggcaaaga gagcgacgga aaagagaagg
     5281 aagaagagcc gctacttcct aaagtgacaa aaggagaaat ggtgacggta gatctacaag
     5341 tccttgaaaa agaaaccaag ccaccacaac cttataccga aggtacgttg atcactgcga
     5401 tgaaaacagc tgggaaaacc gtggatagtg aagaagcgca atccatattg aaagaagtgg
     5461 aaggaatcgg tacagaagcg acacgggcga atattattga aaccttgaaa caaaaggaat
     5521 atatcaaggt agagaaaaat aagcttgtgg tgacgaataa aggggttttg ctttgccaag
     5581 ctgtggaaaa agagccgctg cttacgagtg ccgaaatgac cgccaaatgg gaaagctact
     5641 tgttaaagat tggagaacga aaaggaacac aaaccacttt tctagccaat atccaaaagt
     5701 ttgtctctca cttattggaa gtcgtcccag gacaaatcca atccattgat tttggatcga
     5761 cgttacaaga agtgaaagca gccagtgaaa agcaagaagc cgcgcgtcat ttaggaattt
     5821 gtccaaaatg ccaggaacaa gaagtcttac tctatcaaaa ggtggcagct tgtacgtctg
     5881 aagcctgtga ttttaaatta tggacaacga ttgccaaaaa gaaactcaca gcgacacagc
     5941 tgaaggagat tattcaaaat agccgaacaa gccaaccagt gaaagggtta aaaggtcaga
     6001 agggttcgtt tgaagcaacg attgttttaa aagaagactt cactacaggc tttgaatttt
     6061 ctgaaaagaa aaaaacgaac ttcaagaaaa gaacacgacg cacgacaaaa taatggaggg
     6121 ttaatcttat gcgtaataca acacacaaac aacaagaaat cgaaaatcaa aaagccaaaa
     6181 ttcgttcaga acaagagaaa ttacgtgcca gtaaaagaag acttcagcaa ttaaagaacc
     6241 agcaacggat tcaaaaagaa atcgaattag atcgatagac attaaccttg ttacaaatac
     6301 actatcatga tatatttaat gtagtgtatt tgtaacaaaa ggggggaagt aaatgcaaga
     6361 gaaactgaaa gaattgttgg ctattcgcga tggaaattta tctatgaagg aagccagaaa
     6421 attaggaatc gcagcaacca ctgtacaaag attggtgaat caaggaaaat tgatcaaagt
     6481 tgatcgtggt tattatgtag aggatggtca tggaatagat gatttatttt ggcttcagca
     6541 acgtttttcc aggggaatct tctctcatga aacgacttta gatatttacc agctttctac
     6601 gaatatgccg aaattcattc acttaacttt tccccatgga tataacgttg atcgttcaat
     6661 tacaaaagag cagcagttac aatttcattt cgtgaagaaa gaagtttatg aactaggaaa
     6721 agtagaggga acttcctttc aaggaaatcc tattactatg tatgataaag agcgtacact
     6781 ttgtgatatt tggaatccga gatatgaaat tgaatatgaa atgaaattag aagcattaaa
     6841 agagtacatg gaagacccat tgagagatcc aagtaaatta agagattaca tgacaaaact
     6901 acatgtagaa aaagaaatga aataccacat gcaaagctta tattgatgga ggaaaaagat
     6961 gacaccgaca caatttgcag caaaaacaag aaatttagca aagaggacag gattaaatgc
     7021 acagttagta caacggcatt ttatgatgga tcgtttaatt gaacaaattt ctgaatctaa
     7081 gtataaaaat gatttcattt tgaaaggtgg ctttttatta ggatcaaaat atggaatcga
     7141 tagaaggacg acaattgata tcgacacgac atttagaaat agcaaattaa ctgaagaaaa
     7201 attgaagagt atttttaatg atttaactag gaaacccacg aaagaaggta ttgttttctc
     7261 tattcaagga atgaaggaaa caagagaagc cgatttttat cctggatttc aaattaaatt
     7321 ggtggctaat ttagagaaag tacgtgtgcc atttaaaatg gacgtgacaa caggagattc
     7381 tatttatcct gaagtagata cacactatca ccaattaatg tttgaagata aaaaggtgga
     7441 gattccagct tatccaacag cacaaattat tgcagaaaag ctaagtgcta cgttcagctt
     7501 tggcacagat aattcgagag caaaagatta ctacgactta tttaccattc cgaaactaga
     7561 aaatatcgat acgaaagagt tatacaaaag tgttcgaaat acatttacaa aacgtgggga
     7621 tacaaaaccg ttgtcctttt attatgaaag agaaatggat aagatcatag gaaatgacta
     7681 tttggaaaag cagtgggaaa aatatcgctc agttaacacg tttgcgaatt ccatttcatt
     7741 tacagataca gtcaacgaaa ttgatcacct aatgaaaagt gtgatcaaaa ttgaaaatca
     7801 agaaagaaaa cggcagttta aaagatcaca acaaatgaat caagaaatgg agagataaag
     7861 atgatcaata atgtaacttt agtaggacgc ttaaccaaag atccagattt acgttatacc
     7921 gcgagtggta ctgcagtagc gacttttacc ctagctgtca accgaaattt cacgaatcaa
     7981 aacggcaatc gagaagctga ttttattaac tgtgtgattt ggcgcaaacc agcagaaaca
     8041 atggtgacat tagctaaaaa aggtagcttg attggtgtag ttgggcggat tcaaacgcgg
     8101 acttatgaca accagcaagg acaacgtgtg tatgtgacag aagttgtggc cgataatttc
     8161 caactattag aaagcaaagc agcgacagaa agccgtgcgc acgctgatca atcgtccacc
     8221 agtccaagca ccaccacttt tgaacaacgg gatacagcga cgcctaataa caatggattg
     8281 aacgctttac agaatccatt tgggaatcca tttggtggac aatcaatcga tattagcgat
     8341 gatgatctac cgttctaagt agcattttct attggttgca tttgctacgg tttatgttat
     8401 aatttagtca acaaaaagaa ttttcaatct ataaccaaaa accactagct ggcgggctag
     8461 tggttttctt ttacgtttct attataagaa ttaacgggta ggcttactgt ctttcttggc
     8521 aaatttacaa tcaagccatt tgcagatata gtgcgcggct acttctgcca tgattgcaaa
     8581 aacaaaatca aaaagaattt tcaaacctat aacctcctcc ccttgccaga ttaggccggg
     8641 gtagcagaca gcaagactat tatagcataa ttgaaatatc caaatgattg gatttttgct
     8701 ataattttag cgaggtgatt gtgatgaaca aatatgttgg tttgcttgat aaaatccgag
     8761 taataaaaac gcaaccgtta ttggttcgct ttactttaca aacgattcat gaatcgatta
     8821 attgtgtagt tgcagatatt gaaattatcg ataaattgct aattatggat gatggaaaat
     8881 acaatattgc cgtcacaggt cacttcaata aacgaaatca gctagttatt gctagcatgt
     8941 atgttagaaa tcccgatcat tttacgcgat cgatgggcat ataaaaaaag aagagttgca
     9001 atcctaatgt aggactgcag ctctttttgt tttacataga aacaagaaaa atgaaaaggc
     9061 ctttttagga aggcgcgagc gccttctcaa ttgaaaaacc tctctcactc caaccgccac
     9121 cgccgttcga gaagaaagga agtgaacgtt gcacgttcac taagataaag ggtcttgcaa
     9181 agcaagacaa aatagaaaaa acttcctctc accaaaaggt tcgttcctgt tcgttatctc
     9241 tttattgatt tgccagaccg gcaacaagtt gccttttatc accaaaaagc gacaaggaca
     9301 agggtaaatg gcacgctgcg cgcccttgtc cttgtcgctt ttcgttgatt cattctggcc
     9361 aacaaatcaa taaagaaaga actaacgaaa ggaagtaaaa cctttcctaa gaggaggcag
     9421 aatcaatcat gcgaaagaaa tttgattatt ggggtgttcc cttcagcgaa ttattcccaa
     9481 actatcatgc accacatact gtcgaatgcg attgtggaca aagagcgaaa tgtatcaaaa
     9541 gctatcgttt gtatcaatgt ccaacatgcg gaaaaaaata caccttgtct tatggcgatt
     9601 atattttgat tgatgagaaa ggaaagaaac gctgatgaaa acataacata aattattgac
     9661 tgtaaaagaa gtcgttcaaa ttttggtgga caaagaagtc atttctgaaa aagtagcaga
     9721 agctttactt attccgcggt actggacgat agaaacccct ttagtagagg atgcaccgac
     9781 tatgctattt gaagacctag tgaagattgc aggatatgaa ctgcattatc aagaaaacat
     9841 ggaaacttat gaaaacgaat attggttaac caaattagag aactaaaaag gagaaaaaaa
     9901 tatggctcaa aattactatg acgaatttgt caaattacct ttggataaaa tggcacaaaa
     9961 aatggaggat atgacattcc tataccatga gacacgtgtt ccaaaaaaac attataagga
    10021 aagattgtcg gtagctgtgg aagaaatgat tgaatcaggt gtcgaaatga atctcattgc
    10081 gacgtactat cgaacactgg aagaactgaa aaaacaaaat gcaaaatggt ttttccaagc
    10141 cttactatgc ttggaagtag gtgtcaaacc aagcactatt aaaccttctg aatatcaagc
    10201 tttagaacta acgtatggga aatttgtcga aacgaaaaaa gccaagactg tttcaagtga
    10261 atggttagat tattttgaaa atatcaataa gtatggcgcc tattacacca tgaaaaagga
    10321 ggacaacgaa aatgaataag gtgatttatc aactaacaac ggaagatgtg tcacactttt
    10381 taacaaacaa agaacatttg aatgaagtaa tcagacggat tcaaaaagac tttagttgta
    10441 tggaagaaat gaagtacttt attcaaagag tcgaagaaca attgtatttg gaaagtgaaa
    10501 cgagtcaaac gaaggtcaca gtcagcaata attatttaga cgtgtataaa catcgtcctg
    10561 aaacggtaaa aaaaggaacg gagcttctac gactagaaac agaaaaatac atcatgaatg
    10621 tttacctaga aaggagtgaa ctggtcgaaa actttgtcag ctgcacgatt tgcaggaaac
    10681 actattactc agaatcagaa tattattcaa tttttgagag catggaatta atggaagaag
    10741 aaatggaaga gctaaacagc aaaaatgcga cgatagacga ttattatctt gtatttacta
    10801 gatttttgac gtgtggaacg gaatctatcg tgttaatggg aatggattat gaactcgctg
    10861 tacccgaagc atttttgagg gaatatgtag aagaagtaga taacccaggc gacgactggt
    10921 acacgacttt aactgatttt ttagatacct acacttacga cgatacagaa gaaatccaaa
    10981 atgaacttga ccgtaaacga atgccatatc aacgatttga aaggaaaaaa aggcaataat
    11041 catttatacc gaaaagtgag aatgtgtgaa ataagaacaa aaaccttgaa attaagggaa
    11101 tggttcttat ttcgcaccaa tcgctccaac cgcccccgcc gcttaggtgg tgcgaaataa
    11161 gcgggcaaag cccgataacc taagagaaag aaggagatcc atatgaataa aatcattcct
    11221 ctgttgttcg tactgtgtat tggcgagata cttatgcgtc atggtgcagc tgtacattta
    11281 aacttcgtag tattcttagg aatactagta ttttgtttga ttttttggcg ctttgtgtta
    11341 gcccttgtga gtctactatt caagttgatt gcagccatat ttttcatagg gttatttcga
    11401 tgaaaggaga gagtactaat gccaattgca ataggaaata aacggttacc cgtgacatta
    11461 gatgaaaaga gacaaaaaga attgcagcaa ctaaagcaga agtacggaaa aagtgaatct
    11521 aagattatgt gtattgcgtt agatctattg atcgcgcaag aaaaagcagg atttgatata
    11581 ccagcactaa aaaagtgacg tcacttttca tttgaagaaa caaaagtgat agcacttaat
    11641 tgttcctact atttagaata ttttctatgt cctttttatt ccattttgta tcaataacta
    11701 tgaaatcttt aaagtatcca gatcaatttt tttcttacct gattctaaag attttgtttt
    11761 cttaaacata ttatgatttc ctttcgacta ttttcatata aatgttaaca tagtttatgg
    11821 attcatggaa tgttaatcta atttaataga aaaggataat taagtatata cgttttttct
    11881 tatgtatgtt atactatgta tatacagaaa gaggtgtcta ctatggaaat gttacctata
    11941 aaaaaatggg gaaatagtaa tggccttaga ttgccaaagt atattatgga atatcttgga
    12001 attcatacag aagacaaagt gaagataata caagaagaag taaacggtca gaagcggcta
    12061 attatcgaag cggctaatat agaaaatgaa ttaacaattg aacagttatt tgaaaattac
    12121 actgatgaaa gaaatcatgt gacgattcaa aatttaggtg aagcagtagg taatgaaaaa
    12181 tggtgagaaa acctagacaa ggcgatattc tattattgaa tacagcacct cgatctggac
    12241 acgaacagac aggtaaaaga ccttatattg ttttaagtca tgatattatt gcggattata
    12301 gtaacgttgt tacagttgct ccaatttcta gtacaactag aaactatccg ctatatgtaa
    12361 atataaactc agaatatgaa atgaagacaa caggaaaagt attattagat caactaacga
    12421 caattgatta tgaagccaga gagtgtattt ttttggaaaa agcgcatgat agtttagttg
    12481 aggagttatt gatgaaagtt agaacagttt ttcaaaaagt aagcaaataa aatagaaaca
    12541 gcagcatgat caactattcg tgattgacca tgctgtcttt ttttattcta tatacggttt
    12601 gtctcgtaac attgtagtct tttgcaatct tagcgatcgc aacacctttt tttagatcct
    12661 caacgatgtt tttataaaca agacgacgtt ggggatcttt tgcattggca ctgtatagct
    12721 taggacgtcc tttataaaca ccgttcgctt ttgcaatctt aatgccttgt gcctgacgtc
    12781 gtttagattc ggtacgttct tgttcagcga tcatggccaa aatttgaatg atcaaatctt
    12841 taatgaattt atccaggaga ggattgccaa ttgcttcggc catgatcggt agacttgtga
    12901 tgatcagctg cacatttttc ttcttcaagt aattgactga agcgatgatc tcgtcgtaat
    12961 ttcttccaag tcgatcaata gcttccacaa taaaaatatc ttgatctcgg acgaaatcca
    13021 gtgcttcttg aaaaacaggt cgatgagtaa tcgttgcgcc agattgtttc tcaatgaaaa
    13081 ttctctctgc accgaatttt ttcatttctt caatctgccg ttgttcattt tgatcgatgg
    13141 atgagacacg tatgtatgca actctcaaaa tttttaccta ctttctgcaa ctatttaaaa
    13201 aacaccctaa tcatacacga gaaaaccaga tttttcaatg tcgcggtcgt gtctagttag
    13261 ggtatacctt atatagacaa aaactaatga tggaggaaat agtatgtgtc acttttcttt
    13321 ttaaatggca atattttttg taaataaatg tcaatcaaga aaatttaaac gattatgaca
    13381 ttaaaaatat attttatgaa ttttctagta tataacttga tttatctgtt aagatttatt
    13441 aagaaaacgt tttcttagtt ttgatttcga ggtgatagac aatgaaaaat aagtttattt
    13501 tatctttagg tattttaact attggaatta gtgtaactgg aactacaatt cctacatttg
    13561 ctgatagtac aaatgaacca actactgtag tagataataa ccaagaatta actcctttag
    13621 atagggacgg aatagccatt ggtaacccaa ctaacgactt aactgttact gaaaattatt
    13681 taatagtttc tccattactt gctctagcag cttctaaaac gatacgagtg acaggaacga
    13741 tgatttttga taaagttggt tggccttcta gacctcctca atcacggaat tggaaagaaa
    13801 aaagaaatgg ttattggtac tatggaaaat tgtatttaca aagttataaa tcagctggaa
    13861 acaattggat tgcgacttat tcaggaacct taactagaaa gtagggattt tttatgccag
    13921 aatttgtgaa cacaccaaat ttttatcaag atagcgatat tctaagtaaa attaatttac
    13981 cgaaagaaga atcagcttta caagttcagc gaactttaca ggagacacaa aaaattcaaa
    14041 aatcaatttc taatctagaa gattctgaag aaaaaagaaa aaaaagagac caaaaaacat
    14101 atcaaaaaga aaaataatga ttttaaaaaa agagtgtcta gtcgacattc tttttttcta
    14161 attgaaagtg ttcataaaat cacccatttc tggacacttt ctttttttgc tttatttttc
    14221 aaaaaaacaa ttcatttcat gttcataaac ccataacaat atctatgttc agttaaacta
    14281 tatggataaa gttatgttac aataaaatta atgaaggagt gatttttttg aaaataggat
    14341 acgcccgggt atctactggt ttacaaaatt tggatttaca agaagacagc ctaaataaat
    14401 ttggttgtga aaaaattttt actgatcata tgagtggggc aaaaagtaat cgaccaggat
    14461 tagaaatggc cattgatttt gttcgctctg gcgatacact tgtcgtttgg cgcttagatc
    14521 gtttaggtag aaatatggaa gatttgattt caatcgtcaa tcgactgaat gagcgtggtg
    14581 tgagttttca tagtattcaa gaaaatatta cgatggacaa atcaagttct acaggacagc
    14641 tcatgtttca tttgtttgcg gcatttgcag aatttgagcg taatttgatc ctggaacgat
    14701 ccgcagcagg acgagcagct gctcgtgcaa gaggacggtt tggtggtcgt cctgaaaaat
    14761 tgtctgaaca agatttggaa ttactgaaga ctttagtgga tagcggtaca cctattaaga
    14821 ctattgcgga acggtggaac gtctcaagaa ccaccattta tcgttatttg gcaaaaatgg
    14881 aggagaaaaa tggaaagaca aattctcatt agtttttgtc atttaatgct gaaaattttt
    14941 gccatttatc tagaaaagtg a
//
LOCUS       DAZBTF010000066        14456 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000066 DAZBTF010000000
VERSION     DAZBTF010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.066"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(228..1115)
                     /locus_tag="ACHGNS_002181"
     CDS             complement(228..1115)
                     /locus_tag="ACHGNS_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6938104.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1112..1981)
                     /locus_tag="ACHGNS_002182"
     CDS             complement(1112..1981)
                     /locus_tag="ACHGNS_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6938105.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2118..3404)
                     /locus_tag="ACHGNS_002183"
     CDS             complement(2118..3404)
                     /locus_tag="ACHGNS_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN6938106.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            3677..4255
                     /locus_tag="ACHGNS_002184"
     CDS             3677..4255
                     /locus_tag="ACHGNS_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFN6938107.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            4255..5184
                     /locus_tag="ACHGNS_002185"
     CDS             4255..5184
                     /locus_tag="ACHGNS_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6938108.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            5181..6917
                     /locus_tag="ACHGNS_002186"
     CDS             5181..6917
                     /locus_tag="ACHGNS_002186"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN6938109.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(6980..7816)
                     /gene="pheA"
                     /locus_tag="ACHGNS_002187"
     CDS             complement(6980..7816)
                     /gene="pheA"
                     /locus_tag="ACHGNS_002187"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFN6938110.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(7813..8322)
                     /locus_tag="ACHGNS_002188"
     CDS             complement(7813..8322)
                     /locus_tag="ACHGNS_002188"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFN6938111.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(8315..9610)
                     /gene="aroA"
                     /locus_tag="ACHGNS_002189"
     CDS             complement(8315..9610)
                     /gene="aroA"
                     /locus_tag="ACHGNS_002189"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFN6938112.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(9623..10714)
                     /locus_tag="ACHGNS_002190"
     CDS             complement(9623..10714)
                     /locus_tag="ACHGNS_002190"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFN6938113.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(10731..11897)
                     /gene="aroC"
                     /locus_tag="ACHGNS_002191"
     CDS             complement(10731..11897)
                     /gene="aroC"
                     /locus_tag="ACHGNS_002191"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFN6938114.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(11899..12963)
                     /gene="aroB"
                     /locus_tag="ACHGNS_002192"
     CDS             complement(11899..12963)
                     /gene="aroB"
                     /locus_tag="ACHGNS_002192"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFN6938115.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(12996..14018)
                     /gene="aroF"
                     /locus_tag="ACHGNS_002193"
     CDS             complement(12996..14018)
                     /gene="aroF"
                     /locus_tag="ACHGNS_002193"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFN6938116.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         4118 a         3146 c         2613 g         4579 t
ORIGIN      
        1 tgacaatcag gacagtcatc ggcaatatag cagcaatcac ttctttaaaa ttttttagca
       61 tgtatcctcc tcctttactc tttaagttta ccttaaaatg aaagagtttt cgagcacaaa
      121 cttaaaacgt tatcatttaa tttttctcaa cataaaataa cacacgtccc aaaagtatct
      181 ttttacttgg aaaaatgact ttgaaggaac gcgcttattg tgttttttta ttttacagag
      241 ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa atacgatggc agttggtaaa
      301 gtagcgatga ctaatccagc catgatcatt ccatagtctg gtgtatagga agcacccata
      361 gcagataaaa caagcggaac cgtccgttta tcaggagatt gcaaagctac taagggccat
      421 aaataattgt tccagctact catgaatgtg atgattgcag ccgcggcata ggtattttta
      481 gccgtcggca tatacactct aaaaaaaata cttagttctt ttagaccgtc taatcgagcg
      541 gcttctacta gatcttttgg aaaagattta acattttgtc gaaagaagaa gatcaaaaaa
      601 gcagtgctaa cagatggcag gatgacaacc aagaaactgt tgattcccaa aaccgttccg
      661 ttcaattgtg aaaacattcg atataaaggg atcatcaatg ccgcaaaagg aaccatcatc
      721 gatagcagta gaatattgaa aattaggtct tttcgtttac tttgataaat ctcaaatcca
      781 tatcctgcca ttgacgaaat cagcaatgca aacacagtgg tcaccaaagc gatcatcgct
      841 gaattccaaa gggaacgagt aaaattcagc tcattggaaa acaaattttg aaaattgatg
      901 atcaagttac tgccaatctt tatttttccc gatgtgatat ccacgggtgt attcgtcatt
      961 cccaaaacca tccagacaaa agggaaaatg gaaataaatg ataggatgct taatgctgta
     1021 tactttaata ctgacccaat tgtcgaacga agttctaatg cttgtttttt gacgttgggg
     1081 cgttcgattg tttctttttc taccatattt ttcatcgact ttctcctcct actttcgttt
     1141 ggattatcga caatatcaca ataaaggaaa caataacaaa ggaaactgct gccgcatatc
     1201 caaaattagg cgtgaattta taactcaaat tatagatgta ttgagatagg gtggtcgttg
     1261 catttgctgg tcccccgcca gtaatatttt gtacttcatc aaaaagctgc aatgtcccga
     1321 tcgtcgaagt aatggaagta aacaaaatga ttggttttaa atttgggatc gtcactttga
     1381 taaactgctg ccatttagaa gcaccgtcaa tagatgctgc ttcataaatc tccgaatcaa
     1441 tattttgcat gcctgaaagg aaaaaaatca tattgtatcc agtccaacgc caagtgatcg
     1501 aaagaatgat cagaactctc gcccaaattg gatgagtcaa ccagccaatc ggtgtgtcaa
     1561 tcataccaat gcccaacaac aagttgttta caaggccaga ttgagaaaat agacttttca
     1621 tgatcagaga ataactgacc atcgaagtga tacatggaag aaagatagcg gttcgaaaca
     1681 accctttata tttcaacttt ttatcattta aaaggttgga aataaaaagt gctagaaaca
     1741 acatgatcgg tacttgtaca agtaaataca ggaatgtatt aaaaaaagct tttttaaaag
     1801 ttgcatctgt caacatccgc tgataattac ctaacccgtt aaatgtcata ttgacagggg
     1861 gaccactttg aaaagacgtg atcaaagcca aaaccatcgg aataaaaacc atgattgata
     1921 tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt gaataaatct ttctgcttca
     1981 ttcttttccc tccaattcta tcattgatta tctacatgaa aagacaagaa agacaatatc
     2041 tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct gatgaaaaag cgaagaaatt
     2101 cactttttta ccaccgatca attagccaat tgtgcttcta cttgtttttg tgtatctgct
     2161 agtaccttgt ctgtctcttc tccattgatg attcgatgaa gagcttcagc tacgactgct
     2221 tcaattgcat aggtctcatg tccgtaattc acttctggta tttcagatgt ccatgttgaa
     2281 aaatcgtcaa agatcttttg accactgtaa aattcagaag cttcttgata agcttcttgt
     2341 tcattcgcgc taagcatcgt agatactagt ccgatttctt tcgcaagagt gcccatcagt
     2401 tcttcattcg aagcaaacgt tgcttctagg aaatctttcg ctgaatcttc tccagctact
     2461 cctttgatca cataccagcc agcgccgcca aggttagaag cttgcgcttt ttgcagattt
     2521 tctggaagtg caggtattgg tgcaatcttc cattttccag attggttttc tgctccttgg
     2581 atcgttgaag aataccaagc gccagttggg ctagaagcta ctgctcctga ttgtatcgca
     2641 gttactccag cattccagtc agaggtttgt tcgactagat cttctttcaa aagagtggca
     2701 aacaattcaa gaccatactt taaagatgca ttgtcttgga tcgtgactgt ctccccatct
     2761 tcagatgtat accattctcc tgcctgttgc ataatcatcc ttactcgacc aaggtcagaa
     2821 ggattgactt cagcgatttt ctttcccgtc ttttctttga catctcgcgc tacttgaata
     2881 tactcgtccc acttcagatt ctccatatcc tcttctgtat aacctgcttc tgccatcaga
     2941 tccgtacgat agaagtttgc tgttacgccc gaatcgaaag gaacaccgta tattttatct
     3001 cctactttgt ttaccgcaaa tttatacggt gcaaaatgat cttcctttac aatagcagac
     3061 aaattagcga atgcatctgg ataagaagtc aaatagcctt gtatcctata atcttcaatc
     3121 aagacgatat tgggcaatcc ttctgtattc ccgctcgcta aagcggtgtt cagtttctga
     3181 acgatatcat cctgagacat cgtaacaact tctatctctg tttcttcgtt ctcataaact
     3241 ttttttgctt cattcactgc tttgatattg aatgtctcat cccacgccca gacagtgact
     3301 ttttcttctt tgtctgctgt actactagca cttccgcatc ctgttaagaa acctgatgct
     3361 gccgctaaac aaacgactcc agcaattaac tttctgatgt tcatttttat ccgctccttt
     3421 tccatccatt agttgtttac gtttgctaca ttcatcatac caaaatttga aaacggtttc
     3481 tttattattc agatacttca aaaatttctc ctaaaataat aaaaaaatac aaatcaacta
     3541 tgacaaaagg aataatctta gcatctagac caaagatcga aatggtgagg tttctttaaa
     3601 ataaaaatat ccttgcattt tctcctagaa ataagtattt ttaaattaca agactattct
     3661 taaggcgggt aaaaaaatgc caatacgcgt aattcttatc gatgatgaac agacgatttt
     3721 agatggaatg aaacagttga tcaattggtc tgagcacggg tttcaattaa tagggacttt
     3781 ttccagtccg ctgaaagcgc ttgatttttc tttggtacat gcaatcgata tcgtggtcac
     3841 agatgtaatg atgcctgaat taaatggaat cgaattatcg cggctattaa aacagcaaca
     3901 acctgaaaca cagatcctta tcctctctag ctatgatgaa tttgatatgg ttaaagattc
     3961 ttttaaagaa ggcgtcgctg attatctttt aaagcctcga ttgagtcctg atttttttct
     4021 aaattcctta actgccgttt cacaaaaaat caaaaaaaag cccaacaaaa gcccaatcac
     4081 caatcgttat gagcagatct gcgagaatct ttcacagcag attgctggac aaactcgtcc
     4141 attgcttgac cctcttcttt ttgaacacac cttgtttttc tgttatatac cgaaaaacgg
     4201 ccattcagtt caaaacaagg ctctaaacaa aaacagtcga tcacccattc attgatggat
     4261 agcgacgatt cgtttactat ttatcctgtt tctacctttt cggaagaatc cggctatgta
     4321 atcaatactg attccaaaga tacacttagg aagtttgttc aaaagtttcg tcaagaagtt
     4381 tttccaggtg atttttttgt tgtttctgat tcttttgcaa tccaagatat caatgaaata
     4441 ttccaagaga tcagaaattg caccaaagga caacgtttct atcataaggg aaagaacctt
     4501 gcttatcagc atgaacttct tccgttatta accaaccacg agcagcaagc aagtagttat
     4561 ttaacaaaaa tcgttcataa agactacgct ttagctgtca aagaacttga gaatttcttc
     4621 acgacgatat tggaacagcc tattgatcca gcttatttga aacatgaggc aatcaatcgt
     4681 ttctatgcat tactcaacgc tttggctgac gagcatgttg ggaatgaaga actttctttg
     4741 ctcaagatcg cccttccttc tttattagcc gatgctgagt atttcgaaga tttttctatt
     4801 cttgtcaaag aaacacttga gaaactcacc cacttttctg caaatgcaaa aaaagacaat
     4861 ttcgatatct tgactttgat ctacgagttc gtccaggaaa attatgagaa agaaatcaat
     4921 ttacaacttt tatccgaaaa atatcatttt tcatatagct atctctcagc catctttact
     4981 gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag tccgtatcag taaagccaag
     5041 ctctttttga cggaaacacg gcttactttg acggaaatct gtgagaaagt cggctatacc
     5101 gaactgggtt atttctccag agtttttaaa gaagagacag gaatgacacc ttctcaatac
     5161 cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga gcatgaactc tttgccaaac
     5221 ttctgttcat tttgttcatc ggtctttttc tgcaagccat cgtcatttct ttgtttattt
     5281 atcatcgatc aagggatgcc tatatccagc tgttcaatca atcgaatgat gtggtactaa
     5341 aaaagattca atctgaattt gaaagtttga atgatacaat cgagaataca ttagccgctt
     5401 ttgattctaa tccagctgtc aaaagctatt tttccaatga tccagcccaa catatggaac
     5461 agcttcagca actaagaaca atccaaaaaa tgaatgactc tctttcaaaa atccatccaa
     5521 tgatcgatta cgatgtattg atctttggag aaaatggaag aacttttgtc ggaaacgaca
     5581 tgctcactgc cgtatctgcc gattcttttt ttcaatctgc gattgctcag agagtgaatg
     5641 aacgtgctgc tgatacacaa atgctttttg cacaccacgg attgactctt cgcgataaga
     5701 aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac attgaatcac gtttacggat
     5761 atgctgtttt atcgatctca tccaagcaac tagcgaatct ttttcaatct gtagtaaatc
     5821 ctgagatatc gaagatctcc ttcatgaacg aagaacagac gatcatcgca tcaaatgaag
     5881 aaaatacaat tgggagccga tcaatgattt tcaaaaaact aacagtaggt gaaacaacga
     5941 tttcaaatgg acagcagttg acccgtcttc ctctgtaccg ccaggattgt acattactca
     6001 gtcaagtcga tcttcattct ttatctaacc aaatgggcgt gatttttccg attattttgt
     6061 ataatatcgt gacacttgct tttgctggct cattggtatt tatgtatttg gatcgacaca
     6121 caaaatcgat ctaccgcttg attgattcgc taaaacggct agaggaagat ggcttaaaag
     6181 ctactgttcc tgtacaagga agccatgaag tccaattgct agggaatacg atcaatcaat
     6241 tgctaagtaa tgctcagcat cattatgaac agacgatcca agacgaaaag aagaaacgtt
     6301 cgcttgaaat ccaaactatg caagcacaga tacagcccca ttttatttat aatacgctga
     6361 ctagcttgaa atttttgatt tggcagaaaa aaacagatga agcagttcaa ggaatcgaca
     6421 gtttcattac tttactaaga agtacgattg ggaaaaaaga agaagtgatt ccagtcaagg
     6481 aagaaatcaa aagcgtacaa agttacatcg atcttttgag tatgcgatat ggcgaaagaa
     6541 tctcggctaa aataatgatt cctgatgaac tgcagtcatt atctatgccg aatatgatcc
     6601 ttcaaccaat tatcgaaaac gcctatcttc atgcttttca caataaaacg caaggattca
     6661 tcacggtgta cggcaaaaca aaaaatcagc ttcttttatt cgaagtgatt gataacggcg
     6721 atggcttcga tactgaaaat accaaacaaa aaaatgatca tttttcagga atcggcatca
     6781 gccatgtaga tgagcggata cgcctaatgt atggcgatgc atatggctta acgatccaat
     6841 cagtgattgg gacaggaacg actgtaacga tttgtctacc gcttcttcct aaaaatcaat
     6901 cagaatatca aaaataaaga atagccaagc gctgattttt ttattttaag ttaaataaat
     6961 caatcagtat tcttgacttt catctacgcg tctcttctac tgtcagcact ggataacagc
     7021 ccaatatttg actttctcct ccgagcatct tgatttcttg gagcgcattc tctactaatg
     7081 tcattggacg gtccaacaac aaatcgatca cgaaaaaata ttctcccaag ctagttttca
     7141 atggtcggga ttcaatctta cttaagttga tttcccgcca gccaaaagct gccagcattt
     7201 tatgaagcat ccctggccga ttagcaggta gtgtcaagat gacactcatt tttactggag
     7261 ctgactgctg gctggtcatc tttcggtcac cgacgatcca aaaacgtgtc tggttcaact
     7321 cattatcttg gatattctca gctaagatct ccaatccaac atgctgtgct gtttctaatg
     7381 aagcaatcgc tgctgcgtct tctttcggat gttctgctac atacattgct gcagcagttg
     7441 ttgattctgt tgctactaaa ggaacattag gataatgcgt ttctaaaaac tgttgtgatt
     7501 gtgctagtgc ttgtggatgt gaaaggattt ttgtaatctt tgttgctgga ttgcctaaaa
     7561 gctgctgctt gataggaaga acgatttcac ttttaacttc gacttctgga tgtttggaaa
     7621 gaagatcgat tgtgtgatga acagctcctt ccaatgaatt ctctacagga acgacagcca
     7681 gatcgacttg tttgcgaaac aaagcgcgca aacaaacagg gatggaagca tacgcagtca
     7741 attgttctgt aggaaatagc tgacaagccg cctgataagt gaatgaactt tctggcccta
     7801 gatagctgac gttcatatgc ttttgatcct ttctataatg gtatcgatga tcttttctgg
     7861 tgtttgattg gtcgtgttga ttcgatgtgt cgcactttct tcataccaag aaagtcgttg
     7921 tgccaataat gcttttattt cttctcttgt ttttccaaga gctaaaggtc gaatagacgt
     7981 ttcatcttgt tggatacgat caactagaca gtccgcttct gcctctaagt aaagaacaat
     8041 cggctgtgtt tttaaaaaac gccgattttc tgcactttgg acaatccctc caccagtcgc
     8101 gataattttg tcttcatttg acagctttct taaaatatct gactccactt ttcgaaagct
     8161 tttttcaccg taatagtgaa aatattcggc aatcgacaat tggatatgtt cctcaatctt
     8221 tgtatctaaa tctacatact ctttttgtaa acattgagct aaccctttac cgattgttgt
     8281 ttttccagcg cccatgaaac caatcagcaa aatgctagcc atgagcttct cctcctgaca
     8341 caagttctgc taaatcgtca aaaaatgccg gataagagat tgaaacagct tcagctcctt
     8401 ctagttcgca aggtccatca gctatcaaag cagctacttg cagcatcata ccgattcgat
     8461 gatcgcctct gctggacact tttgcgccat gcagaggtgt cggcccatgg atgatcagac
     8521 cgtctgctgt cgcttttatc tttgctccca ttttctgtaa ttcttctgcc accgcatcaa
     8581 ttctgttggt ttcttttact tttaattctt ctgcgtcttt gatgatcgtg attccttctg
     8641 cttgagtagc cactaaagct agaatgggca attcatcaat cagacgcgga atgatttctc
     8701 caccaatcgt tgctttcttc aaatgactcg tcttgacact taaatcagcc gattggttct
     8761 gttcattgtg attcgtctgg gtaatcgctg cgcccatctc ctccaacaca tctaagatac
     8821 cagttcttgt tggattgacc ccgacatttt ttaacaacag ttggctttcc ggaacaagaa
     8881 gtcctgctgc tagaaagaaa gcagctgatg aaatatcacc tggaacagtt atctgttgac
     8941 cagttaattt ttgcggtccg gtcactatga tcgttttatc ttccactgtg atccttccgc
     9001 caaattgacg aatcatttct tccgtatggt tacgggaacg ttctttttca acgattacgg
     9061 atgtgccttc tgcttgtaat gccgcaaaca gcaaagcaga tttgacttgc gcactggcta
     9121 caggcatatt gtatcggata ggtgataaag agcgggttcc tgtcacagtg attggtagat
     9181 actggtcatt ttcttctcct tgcaagtcag cgcccatctc gcgtaaaggt tccatcactc
     9241 gccccattgg acgtttagac aacgaagcat ccccgaacag agtagtcgta aaaggctgtc
     9301 cagccaaaat acccatcaat aaacgtatgg tcgtaccgga gtttcccatg tcaattgatt
     9361 cggttggttg gacaaatgag tggctaccgc gaccatgaat cttgatcacc tcttcctctt
     9421 cttcgatctc gacacctaat tgttggaagg catgtaaagt actcagacaa tcttctgcgc
     9481 gcaaaaaatt ttgtatagtc gtcgttcctt cagcaattgc tccaaacatg atgctgcgat
     9541 gggaaattga tttatctgct ggtatcctaa cagtccctct taatccatgt atttgttgta
     9601 ataattgcat ttatccagcc tcctatttga tccagcaatg ataactggta ttagacataa
     9661 tgattctttt tgcttgtaac aaatcctgtt ggtttttaaa actgatttgc agtattccta
     9721 aaatatcttc cctggtctct tggatcttca aattaatcaa agaaactccc gcttttccga
     9781 tcagtccagt tacttctgca ataacgccag gttcatctgg cacatcgata aataaatcaa
     9841 aaaaggcagg aatcgcgcct tgcttatgga caggcaactg gtctcgactt tcttttgccc
     9901 gattgaaaaa agaaaagatt tccgattggt tctcagtcat gatccattct gctatttgtg
     9961 tcatttcttg ttgccaatct gccaatcgtt ccaataatat ctgcttattg ctcaacaaaa
    10021 tatctgtcca catgacagga tctgaggaag cgatcctagt gatatctcga aagcctcctg
    10081 ctgccagttg tttggctcgc ggatactctt gattgaattg ttctgcctga ttgactaagc
    10141 cagctgccag aatatgtgga aaatgactta gcattcctgt tatacggtca tgttctcttg
    10201 ctgtaagagt tacatatttt gctttggttc cgcaaaatag ttcttgcagt ttttcaacat
    10261 cttttttcgt cttttccaaa ggtgtaaaga tgtaataggc attttcaaat agtttagcat
    10321 ctgctgcctg tatacctgat ttatgagaac cagccattgg atgtccaccg acaaaccgaa
    10381 gatttttctg ctcagcggat gtcacgactt gctgcttcgt actgctgaca tccgtaatga
    10441 tgacgtgatc agacaagttg attctttcaa ggacatcaag ataagctaag gttgtacgga
    10501 cgggaacggc taataagata acgtctgctt gttctgcacc tgtttcaaaa tcacttgcaa
    10561 cgtaatcaac gatctgctgt tctactgctt gctcacgggt ttttgactga ctatcccaac
    10621 caactactat cagttctgga ttggtctgac gaatacaaag tgccatagaa gcaccaatca
    10681 gtccaagccc cacgataaaa acatgttgtg ccatcttatt tgcccccttt ttagtaatgt
    10741 ttcaagtagt cacggtactc cttcactgca gctttcaact caacaagcga atcacttggg
    10801 aattttgaca atagctctgt cgctaccaca gtggctacaa cattttccgc aacgacgcta
    10861 gctgcaggaa ctgctgtact atccgaacgt tctacacttg ctttgtaagg ttcttttgta
    10921 tcgatatcaa cactttgaag tggcttgtac aatgtaggaa tgggtttcat gaccccgcga
    10981 atgacaagtg gttcgccatt ggtcattccg ccttcgaagc cgcctagatt attggtacgt
    11041 cggaaatagc ctctttcttc ttcccatagg atctcatcca tcacttctga gcctggtaat
    11101 gaaccagaag tgaatccgac cccaaattct acgcctttaa aggcattgat actaaccaca
    11161 gcttgtgcga ttttggcatc tagcttcgtg tcccattgta cgtagcttcc taatccagct
    11221 ggaacgcctc caacgagcac ttctacaatt cctccgatcg tatctccatt tttctttgtc
    11281 tgatcgatca aatcttttat tctttgctca actgctggat caacaactcg tacttctgac
    11341 gcttcagaaa tttctttgat ttcttgaatc gagatatttc caggtatttc tgcttggatt
    11401 ccaccaagac aaatcacgtg tcctgctacg atgatgtcaa gttcttccag aattttcttg
    11461 gcaattgcgc cgattgctac acgcatggtc gtctctcttg cagaggaccg ttcaagcaca
    11521 ttccgaagat ctcgatgatg gtatttcata ccgcctacca aatccgcgtg tcctggacgc
    11581 ggacgtgcaa ctcgccgcag tgattggtct ttttcaggca cttctgctac agacatgaca
    11641 gttgtccaat ttttccaatc tttattttct accactaaag tgacaggtga accaattgtt
    11701 ttgccgtgac ggatgccaga cgtgatccga acacgatctt tttcgatctt catccttcct
    11761 cctctgccgt aaccagcttg tctgcgagcg agttcgtgat tgatttcttc ttctgacaga
    11821 ggtaatcctg ctggtagtcc ttcaataatg gctgttaatt ctggtccatg ggattctccc
    11881 gcagtcatat aacgcatctt acagtcctcc taagtaatct ttgaatgtct ctgtgggaac
    11941 aggaacgatt tttgcttgcc caatcttttc tagcaagatg atattcaatt ggcttcctct
    12001 tgctttctta tcatgggtta tcgcgtggaa taattcatct ggatgactta gatcgtacgt
    12061 aatcggcaga tggaattttt gaagtatcct ttctaaagca gctgtcgatc cagttggaga
    12121 caagccgatt ttttctgcat gagaagtgat ggctaccatt ccaatagcta ctccttctcc
    12181 atgactgact accccatatc ctgctgtatt ttcaattgca tggccaattg tatgaccaaa
    12241 atttaaaata agtcgatttc cttggtcaaa gacatcttct tccactactt ttcgtttgat
    12301 ttccaacgta gcagtaatga ttggaacagc atgttccaga agatcttttt cgtctttcag
    12361 ctcgttcagc tgtttccaaa gcgttgcatc tgctatagct gctgatttga tgatttcagc
    12421 gattccttct cgtaaacgac gtaccggcaa agtttttaga acctcaggat caatcaatac
    12481 accagctggc tggctaaatg ttccgacaag gtttttcgct ttgtttgtat tgactgctgt
    12541 cttcccgcca atactagaat ctacttgtgc aagtagcgta gtcggaattt gaacgaaatg
    12601 gatcccacgc atgaacgtcg aggccacgaa tgctgctaga tcacccacca ttcctccacc
    12661 aagtgcaagt atcccatcgc tgcgggtaaa atcattatct gctaaaaaat cgtataactc
    12721 agctgcttgt tctaaagatt tactggtctc acccgccggt acgattgaaa ggactggttc
    12781 aaaattagct tgtctcaatt ggaacatgat tttttctgca tatagtggag caacgtttgt
    12841 atcagtgatc acagctactt tttgaggtct ccaaatcgtt tgaagccatg atccagcctg
    12901 ttctagtgcg cctttttcaa tatgaatatc gtatttttgg ttatcaacat gtacagtaag
    12961 cattcgtttc acttctttct tttaaaaagc tggaattaat cgagtgtctg aatcttttcc
    13021 attgcttctt tcatgatcgc aacttctttc atcatgcgtg agtaagtctt ttcattcaac
    13081 gattggggac cgtctgacca agcatttgca ggatcaggat ggatctctac gatcatgcca
    13141 tctgctccgc ttgccacacc cgcacgagcc attggtggta caagttccca aattcctgta
    13201 ccatgacttg gatcaacaat cacagggaaa tgagttaatt ttttcatcaa tgggacagca
    13261 cttaaatcaa acgtattacg tgtcgctgtt tcgtaagtcc gaatcccacg ttcgataaaa
    13321 atgacaggag atttgtcttc gactgcaatg tattctgcag cattaagcca ttcattgatc
    13381 gtaccggaaa ttccacgttt caatccaaca ggtttacctg ttttaccgac agcagatagc
    13441 agtttaaaat tttgcatatt acgtgcgcca atctgtagga tatcgctgta ctcagccacc
    13501 atatcgatat gcccttcatc catcacttca gtaatcactt tcattccaaa ttcatcagcc
    13561 gcttgacgga tgtatttcaa tccttcttct tctaaacctt ggaaagcata tggagaagta
    13621 cgtggtttga aggcaccacc tcgcagaatt tttgcgcccc cggctttggc cattcgcgca
    13681 cattctctga tttggtctaa tccttcgata gaacaaggac cagccatcgt cacgaaactg
    13741 ccgtcaccaa tttttactcc gtctacatcc acgactgtat tgccaggatg gaattctcga
    13801 gaagtcaatt tgtatgtgtt taaaatacgc acagcatttt ctactccttc atagcgtgta
    13861 aatgctcctt cttgtaaact tcgcgtatca ccttttagtc caatcactac tcgttctgtt
    13921 ccttgatcga ttgagacttt gaatccttct ttttcgatat gatcgacgac tttttttatt
    13981 tgttcttccg ttgcttcttt tttcataatt actatcattg tttttcccgc tttcttttgt
    14041 ttacttatct atcttggctg attttctcta ctttttcaag gtggttttac attaattcct
    14101 actaaatgac cgtgtcctgt tgttctcatt ttcgctcctt ctttcctact tatgacccag
    14161 ataatcaaca aaaaaagatg tccctgtagg aaattgcctc tacagtgaac atcttcaacg
    14221 agccttaaaa agcgctgcaa aaatataaaa tagccactat agatgaattt ctctacgtgg
    14281 ctgtaagtcg tgtttaaaaa tctttagcca tcatagatac aaacctggtt tgtttgtacc
    14341 cacgaagtca tgaatacaaa cctatctaaa atagctaaag taatacgtat tcagttttgt
    14401 aagttttcta gatttcataa aaaatcgctc caaactgatg taagaattgt acctag
//
LOCUS       DAZBTF010000067        14426 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000067 DAZBTF010000000
VERSION     DAZBTF010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.067"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(602..1786)
                     /locus_tag="ACHGNS_002194"
     CDS             complement(602..1786)
                     /locus_tag="ACHGNS_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFN6938117.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(1811..2818)
                     /locus_tag="ACHGNS_002195"
     CDS             complement(1811..2818)
                     /locus_tag="ACHGNS_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN6938118.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(2990..3349)
                     /gene="comGG"
                     /locus_tag="ACHGNS_002196"
     CDS             complement(2990..3349)
                     /gene="comGG"
                     /locus_tag="ACHGNS_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFN6938119.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(3321..3746)
                     /gene="comGF"
                     /locus_tag="ACHGNS_002197"
     CDS             complement(3321..3746)
                     /gene="comGF"
                     /locus_tag="ACHGNS_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFN6938120.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            complement(3709..4056)
                     /locus_tag="ACHGNS_002198"
     CDS             complement(3709..4056)
                     /locus_tag="ACHGNS_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938121.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            complement(4028..4498)
                     /gene="comGD"
                     /locus_tag="ACHGNS_002199"
     CDS             complement(4028..4498)
                     /gene="comGD"
                     /locus_tag="ACHGNS_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFN6938122.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(4473..4751)
                     /gene="comGC"
                     /locus_tag="ACHGNS_002200"
     CDS             complement(4473..4751)
                     /gene="comGC"
                     /locus_tag="ACHGNS_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFN6938123.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(4748..5797)
                     /gene="comGB"
                     /locus_tag="ACHGNS_002201"
     CDS             complement(4748..5797)
                     /gene="comGB"
                     /locus_tag="ACHGNS_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFN6938124.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(5754..6683)
                     /gene="comGA"
                     /locus_tag="ACHGNS_002202"
     CDS             complement(5754..6683)
                     /gene="comGA"
                     /locus_tag="ACHGNS_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFN6938125.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            6815..8569
                     /gene="ade"
                     /locus_tag="ACHGNS_002203"
     CDS             6815..8569
                     /gene="ade"
                     /locus_tag="ACHGNS_002203"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFN6938126.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(8667..9329)
                     /gene="trmB"
                     /locus_tag="ACHGNS_002204"
     CDS             complement(8667..9329)
                     /gene="trmB"
                     /locus_tag="ACHGNS_002204"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFN6938127.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(9399..10181)
                     /locus_tag="ACHGNS_002205"
     CDS             complement(9399..10181)
                     /locus_tag="ACHGNS_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFN6938128.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(10385..11596)
                     /locus_tag="ACHGNS_002206"
     CDS             complement(10385..11596)
                     /locus_tag="ACHGNS_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6938129.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(11589..12326)
                     /locus_tag="ACHGNS_002207"
     CDS             complement(11589..12326)
                     /locus_tag="ACHGNS_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6938130.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            12464..12898
                     /locus_tag="ACHGNS_002208"
     CDS             12464..12898
                     /locus_tag="ACHGNS_002208"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFN6938131.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            12891..13235
                     /locus_tag="ACHGNS_002209"
     CDS             12891..13235
                     /locus_tag="ACHGNS_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFN6938132.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            13415..14425
                     /locus_tag="ACHGNS_002210"
     CDS             13415..14425
                     /locus_tag="ACHGNS_002210"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFN6938133.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
BASE COUNT         4588 a         2936 c         2434 g         4468 t
ORIGIN      
        1 cgagtaattt tctagtatga ttatccattg agagactcct ttggtagggt gacgttttgg
       61 tcgactaaat tttaccaaaa gtgagtctct ttttgagttg aaacgaagaa aaaagtgtta
      121 gtagaaaatc gctttcgatt ttccaccaac acaaaatatt atagaaccgt tttttcgcaa
      181 caaaaaactt gattttcata cctgtgcttt cacgattcag gcaaagtaca ggtggaaaat
      241 caagttttta ttattcgatc aatgaatgtt tcggatcggt tttctagcta aacataaagc
      301 gaagaataaa gtcgacagcc cacgaggctg atctaaaaat gttttttctt tttacatatg
      361 taatgtccct tataaagatg aatgaaaata aaatattggg attttcctga aaatcaataa
      421 ataaaatgcc agtcttttcc cttttgacca cttttccaag caaaattccc taagaagatc
      481 ctgtaaaaaa gaagggaaat tgaagttaaa ttaccatttt gcggtcttct ccgcaaaaaa
      541 aacacatgca agaattccct cttgcatgcg tttcatcttt tatcctaacg aatgctttgg
      601 cttagccgcg cagacgctcc acatcacggg caatcattag ttcctcatct gttgggatca
      661 ctaatactct tacttttgat tcttttgtag agatatctac ttcttcacca cgcaattcat
      721 tcaacgctgg atcgatttca caaccaaacc aagacatacc tttgatgatt tcactacgta
      781 catgtccgtc attctcgccg ataccagcag taaagacaat tgcatcaaca ccattcatcg
      841 ttgtcacata accgccgatg tattttcgga tgcggtccac gaaaatgtca taagctaaac
      901 gaatatgttc tttttcatag ttcttttcaa gatcacgcat atcactagac aatcctgtta
      961 ggcctagcag accagatttc ttgttcaaaa cattgatcat ttcatcaata tcaatctcta
     1021 atttttccat taaatatggg agaacagctg gatcgatatc acctgaacgt gtacccattg
     1081 tcaccccagc taatggtgtg aatcccatag aagtatccat ggatttacct ccatcgactg
     1141 ctgtgatcga tacaccattt cctaagtggc aagtgatgat ttttagttct tcgatcggtt
     1201 tgttcaataa ttttgccgca cgttccgaca cataacgatg acttgtgcca tggaagccat
     1261 attttcttac tttgaaatct tcataataag acaccggtaa gctatataaa taattttgct
     1321 ttggcattgt tgagtggaaa gatgtatcga atacagccac actcaaaatg tcaggtaaaa
     1381 tctttttaaa cgctttgatc cccattaagt ttgcaggatt atgcaaagga gcaaattctg
     1441 ctaattcagc aatgcggtcc attactttct catcgatcac tactgaatcc ccataagttt
     1501 ctcctccgtg aacaacacgg tgccctactc cggtgatttc atcataagag cttaaaattt
     1561 gcagttcaat caactgatct aacagcattt ttactgctac ttcgtggttt tcgatatcca
     1621 tgatttgttc gaatttttga ttatcgccgt acttgatggt aaaaatagaa tcttgtaaac
     1681 cgatacgttc gacgatccct ttcgcaacta cggtttcttc tggcatttga tataattgcc
     1741 atttcaaact tgaactccca gcattgattg caattgtttt agacatatga ctctccttct
     1801 cgttgaatga ttacagagca gatgatttcc agtttttaaa ctcgttgaaa aattccgtca
     1861 ccttctctgg ttcttttaat gaagcaagtt tcacaagcaa gacttctttt acttgttgtg
     1921 cttgtggtcc ttttttctgt aagatcaaaa tacttttttg cgattgttta ttacggaaca
     1981 attcatctgg caattgaatc attccttgaa gataaccttc ttctttaaac cattttttga
     2041 tttcttcact ttgatccgtt tctaaaaatc cactcggcat caaaaacaaa ccaaaaccat
     2101 ccggcttcac atatttcatt gattgctcta gcaataaatg atgtgcataa ctatggcctt
     2161 cttcggtact tgttaaaaac tcttttgctt tttcgtcatt cggataataa ccgattggca
     2221 aatcgctgat tgcaaaatcg acaggatcta ttaataattc ctgcaacccg tcttgatgaa
     2281 agtattgaac atttgcttgt gtcaaatcac ttgtagaagc tgcgacagcc aacaatgtat
     2341 catcaatatc cacaccaatt ccttctgttt ggtaccccgc attcgatagg tttaaaagaa
     2401 ctgttaataa caagttgccc attcctgccg caatatccag aatcttaacc ggtgcttttt
     2461 tatttgtgta tagttgttca ataagaaaaa caaataaaaa accgatacta tcaggagtga
     2521 gttgatggtt cgcctgcaaa tgttctgtct ggctgccctt caataaaagt aattgagaca
     2581 gtctgcgcca ttcttctggc tcgaaagaaa gtttcttcaa ctcttcataa agagctgtga
     2641 tccgttgcgt tgtctctttt gtcggcactc catccaccac acgtacttga taatcatcta
     2701 ttaaattttc tgcattttcg acataggctt ctaaaaagga agttcccaat gcattctgta
     2761 aaagctgaat agcttccagg ttttgattaa acccttgttc cattttttct ggtaacatgg
     2821 tgcacctcat ttctagatat attataacaa ttttttcacc tgtttaattc tatcgaacaa
     2881 ttgacaaaat ttcaatcgtt ttcactgttt tttatttgtg aaacggcaaa cattttttta
     2941 ttaaaaacag tgttataaca gaaaagaggg cgttttcacg aacattccat cattctgtac
     3001 tagtatctgt atcatttttt acttcttctg tgaaccgaaa gataaacggt gagacttcca
     3061 cctctaagaa tagggactgt tgtacccact gatatgttac agatccagtt gtgtagtaaa
     3121 aaactccttc cgaactttcg gcaagcgtag actgttccga taaagccatc tgcttcatta
     3181 tttttgctat ataataattt ttgattcgta tattaagatc cattgacaaa cggtagcttt
     3241 ctaatgctgc catcaataaa aacgtacata aaagaaataa aagcaagaca gaaaataaaa
     3301 tagagccctt ataacatttt ctactgatcg ttttttgtcc atatggcata tccatcttct
     3361 ccattttcaa agtgtacata aaaagaaatt gtgtgttctt tttccaataa ttgaagctca
     3421 gtgatacttg ttaacatagg ttgatgtccg ccggaagttc ttcttttacg tatcattgcc
     3481 tgatatcttt cgatgatata ctgtttatcc tcgatcgtgt agtgcagttg ctgatttttc
     3541 acagagacat tgtgactgct cttgagttct tcttctaatt gtgctaaaaa tattagccac
     3601 tctttttgat ctttacgctg caaaaattca cttacttgga tggtttgctg aatcaaaaga
     3661 ttgaatgtga gcaatagact gctcaatatc atcagtccaa ttagacattc aatcagcgta
     3721 aatgctggaa ttacttgttt tttcaatact gatcgcttct ttctccatat tgacaaacac
     3781 tttttcgatt actccattat tatcgacaat ttggatatcg tatgggatca tttcttccct
     3841 tcgctctggt cttttgtgat acatccgctg cgtcttgata tcttcataaa tcattcgaat
     3901 aaatagcagt tcctccccac ttttcttttc tgcctgaagc aaaaacagct ggccaaagct
     3961 cactaagtag acacagacag aagcgatcat aagcgctgta agggactcca acaaaacaaa
     4021 tcctgtttta ttcaattctt tttgtatagc gaccactccc tatctggaat tgatacgtaa
     4081 tcgtccgctc tttttcttcc caataaaatt gataagcttt tagtgaactt tcattcccgg
     4141 ttctagcagc aaaagtaata gaagcatggc gtttcaaggt aatctcatca ggaatatcta
     4201 atacttccca atttatttta tctggattcg gaacatgaaa aatgatttga ttctcttcac
     4261 tattccaata aaagcctgtt tggatctgat tcacaatagc gattttttgc gtagaataga
     4321 ttcgtttttc aaattggccg aaaaattgat agactgccaa ttcattcttc catgcagaaa
     4381 atgcgagaac tggaaaagaa aggatcgttg taagtaataa aatcagtaag agtgattcaa
     4441 acaacgtata tcctgatgct aaaaccttac cttcaaggcc gctgatagat ttctgcatgt
     4501 tctcgtttga tatagccttc attcaacaat tcctccacag taggaactct tccattattt
     4561 tgaagcgcat agagctcttt ttgtgtgtct accaactgga tgatcgcttc acgactttct
     4621 tggtctacat gattcctata acgcgacaaa ttagggacga acaacaacac taatacactg
     4681 atgatcagta agacgattag catttcgatc aatgtaaaac cgctataata agatattttt
     4741 ctctttttca tggcaaaacc tcctctatgc ttccgtaaac aggtaataaa acagcggcat
     4801 aaactaataa aatcaaagcc gctacgccca aaaaaatgat tggctgaata aaatgcatcc
     4861 agtgatgaag acgtttgaaa agctgttttc ttgtccattc gctgtaaaaa atcagttctt
     4921 tacctaaact tccctttgcc tctccctgta aaacaatttt actgaattct tctgatagaa
     4981 aacgataatt ctggaactgc tcagccaatg acatacctgc ctccaatcct tgaatcaact
     5041 tagaggcaag atgctgaatc agactttctt gtttcgtttc ctttaagcag actatgattt
     5101 gccttaattc gaaaccttca tgaaaaagct ttccaatctc tagtgacaaa taagccgatt
     5161 gatacaagga atatatcggt ccaatcaata attgtctgct tatccattca ctctgatcaa
     5221 caggagacaa cttcatcaga cgatacctta ctagtaaagc caatagagta accgctagga
     5281 tgaatagtag aacataccaa tgaaagactt gtatcagctg aataccccaa tgctcttgca
     5341 tgaccattcc tgaaccaata agctgaggca aaacgaacaa ccgcatacta aacaagatac
     5401 ctagcaaaaa ggtgagcaat agcatcggat atgcaagcag ctttttcaaa tcattgcgaa
     5461 atccttcaac aagccgaaac tgtttggcaa tttccaacaa agttgtcggc agattcccat
     5521 gacgttcagc taactcgatc tgtaccagtt gctctgctcc atatcctatt tgcgaaagta
     5581 cctcactaaa cgcttgccct tcctttagct gatgttggat atttgccaaa ataacaggtg
     5641 gaaacgaacg gatcgtgagt aataaataaa gggattcttg cagactaaaa ccagataaaa
     5701 gcagttctcc catggtttgt gcaaactggt tttgctggct tcttgttaat cctttagaat
     5761 agttctgact gccaaatatt ttcctgagct tcttcataac tttcctccca tgattttttt
     5821 actgattctt cctccataaa ttgataactc cataaaacag aaggatggtg attggcatct
     5881 tccaaaagtt cttgataaat gacaccagct aaacattcag atagctcttc ttttttcttt
     5941 gtcagttcat ggattctgac caatgtacct tcaagattgc gtgcatgtac ggttgcaaaa
     6001 acccggtgcc ctgttagtgc tgctcttacc gcagcatgtg cagttgattc atctcttatc
     6061 tcgccaatga ttagcaagtc tggacgatgc cgtaatgcga gtctcaaaag tgtatcataa
     6121 gtctgtgcta ttttcggatt gatctgtagc tgcagaaaaa acggttcttc tatctctaca
     6181 ggatcttcga tagtaatcac ttgcaatttt tcttccaaag ccaaccgata cattagagaa
     6241 gtcttgcctg atccgactgg tccgctgaaa aggtataatc cacgtctgac ggttttctgt
     6301 aaaatagttg caaaatctcg agaactaaag caacgatggg gtgtttcaaa agtaccgtat
     6361 aatagccgaa tcactaaact ctctcgttga aggtaatctc ctacagaaga caaccgcaga
     6421 cgaacttcct gtttttctaa agaataagta aatgcaccta actgagcttt tcttttttct
     6481 ccgatatcca tctgtcccaa atacttaaat cttgagatca gttgctgtcc atctgccgaa
     6541 gagactttct tccagaatgt tcgttttttt ccatagcgaa aatagatgta aaatacttct
     6601 gaagctggca atatatatac gtcttgggca tgtttggcaa aactttcact tatcatttga
     6661 tccgataatc gttttatttc cattattctc ccctccacaa aaaacatacg tctttttgat
     6721 ctttttgttt caaataaaat gaatttacaa aaaaatattg gcaaaaaccg aatttatgtt
     6781 atacttataa aaatttatct tgggaggcac tccattggaa acaacattaa ggaaatatat
     6841 cgaagtagca agtaaaaaaa agcctgctga cctagtgatc aagcatgcta agatcgtcaa
     6901 cgtctttacg aaagaaatca tggaaaaaga tgtagcaatc tgcgaaggaa tgatcgtagg
     6961 aatcggtgaa tacgaaggaa aaagcgtaca taatgctaat gggcaatatc tagtgccagg
     7021 attcatcgat ggccatgtgc atatcgaaag ctcactttta tcaccaagcg aattttcaaa
     7081 agtatccttg cttcatggag ttactactgt agtgacagat ccccatgaaa taggaaacat
     7141 tgctgggagt acaggtcttg agtttatgat agaagatgca agacaaacac tcatgaatat
     7201 cttttttatg ctaccttctt gtgtacctgt aacccctttt gagaccaatg gtgcaaattt
     7261 aaatgcaaaa aaattagccc cttttttaca aaaacccgaa gttttaggct tagctgaagt
     7321 catgaactat caagcagtag caaataatga aacagacatc ctagaaaaga tccaattgat
     7381 gcaccagcat aagaaaaaaa ttgacgggca tgcagctggt atcggcatgg aagagttaaa
     7441 tgtttatcct gcagcaggta ttcggacgga tcatgaagct acaactgcaa aagaagctaa
     7501 agaacgtttg gatcttggta tgtatttaat ggttcgagaa gggaccgttg caaaagatct
     7561 ggcatcgcta ctaccggcga tcacacctga aaattcaaga agatgcttct ttgtcaccga
     7621 tgataaactg atcaatgatt tagtaaaaga aggatcaatc gatcatatcg tccgtcaagc
     7681 tattgcactt ggaatcgatc cattacaagc cattcagatg gcaacattga atgcagcaga
     7741 atgttttgat ttgaaaacac taggtgcgat tgcccctggg tatcaagctg atttcttctt
     7801 aacagacgat cttaccaagc tgccaattca tgaagttttc tataagggaa cattagttgt
     7861 tcaatctggg caactccaag accatttatt tgctgatcat tcaaattcat atacgtgttg
     7921 tctgccaaaa ctaaacgctc aaccattaaa tccacattcg ttagagctgc ttctttcttc
     7981 ttcacaagcc catgtgatcg aaattatccc gaatagtcta gtgactaaag atttggtaga
     8041 aaaagtggac cgacaaggcg aacgattcat tccttcaatc gaaaaagacc aattaaagat
     8101 tgcggtgatc gagcgtcacc atcaaacggg aaacattgga ttaggcatag tgaaaggatt
     8161 tcagctgaaa caaggagcga tagcgacaac gattgcccat gactcgcata atctagttgt
     8221 cgttgggaca aacgacgagg atatgctgta tgctgctaat cttttgattc aaaaaggtgg
     8281 cgggatgacc gttgtcaaca atcagcagga aatagcttgt gtttctcttc ctataggcgg
     8341 aatcatgaca caggaacctt tttcgatcgt ctatgaacag ttgaatgaat taataactaa
     8401 agcacatcaa ctaggagcag ctaaaacctt tgatcctttt ttgacacttt ctttcttaac
     8461 attatctgtc atacccgaat tgaagatcac cgacaaagga ttattttcat tttctcgctt
     8521 ccaattgatt ccaccttgtg gaaaaaccga tgaaataaat acattttaaa tcaaataaat
     8581 cccagaaaat acattacatc tgtaatgttc attttctggg atttatttat ggatattaat
     8641 gtttttatga aagcagaaag ttagtttcac agacttttct ttttggtctt atcttgaaat
     8701 tgagcttcta ctcgataaat acgttgacct tttgaagaaa atttttcttc atactccgtc
     8761 ataatattgc cttcaaattg actttgatgt aaatctaacc atacctgttt gaggatcatg
     8821 ccatattgag aaaaacttgc cagagaatac tcaaacaatc cttgattatc tgttttgaaa
     8881 tggatttctc catttggacg aagaatctgt tcatctacag caagaaaagt cttatatgtc
     8941 aatcgccgtt tttcatgacg tttttttggc catggatcgg aaaaattcag atagatttga
     9001 tctacttcgc tgtcagcgaa atactctgtc aacgctgatc catccacatg tagaagtttc
     9061 aggttaggaa gcggttgttc gatcaacttg tctagtgcaa tcgaaacaac actgacctgc
     9121 atttctactc caatataatt gatttcagga tgggcttttg ccattccagt gatgaactgg
     9181 cctttcccca taccaatctc aatgtggatt ggatgatcgt tttcaaacaa ttcattccat
     9241 tttcccttcc acaatgttgg atcactaatt acaaaattcg ggtgagccgc taacatttcc
     9301 gctgctcccg gacgatttcg gacacgcatt atttatctcc attcctttcc tctaattaaa
     9361 aaggtctggc caaatctccg cagagtttgg gccaaacctc agccactgaa aattcgtttt
     9421 aattgtaaaa tttcttcgtt catccgtcga tgatcttcac gtttgcaata tctggtgatt
     9481 tcttggagca aattcatggc cgcataccaa tgaaattttt caagcatttc gtcagtcggg
     9541 cgtatgccat aactgatcag ccactgattc cagctagaca gtggcacata atttcctaaa
     9601 atcgttgcaa tatccaccgc agggtctgca aacatgattg aatcccaatc gactagatat
     9661 aaataatttt tacatactaa ccaattacga tggtttacat ctccgtgtac tactgataag
     9721 ttttccttat ctattgacgg gatctgctca cgaagaaaac gatggacaat ttgcagaaat
     9781 tgatttttct ggagttcttc cggtaattct ttttcatatt tttgcagcat cttctccggc
     9841 gtcatgacac gaccaccgat acgggtcagc atggttttaa gagaatttga atgatgtaaa
     9901 tggtacaaaa catcaatcac gtcgtttctt tgaccaatct cattaggagt cagcaagcga
     9961 ccttccagcc actcttgtgc tgtcaaagta tcgccattcc ccgttcgttt tgtccaaaca
    10021 agttttggtg taattccttc ccttgaaaga gcagccaaca tcggagtcgt gtttcgttta
    10081 ataaagactt tgtcctcatc acgcatgcct atgtatgttt ttcctgtgtc gcctttgatg
    10141 ggacgtaacc gccactcttg gtctaactga aactccatca tgcattctcc cgtcctctta
    10201 ctctttgttt tgcagtactt attattctag tcgcctgaac actacacgtc aagcacatat
    10261 acgttctttt tcatttaatt gccattcaaa aaaccagctt ttctcaaaaa aaatagaacg
    10321 aacagcattc ttactgtcaa cgctctattt ttcattctca tttttaccct acacagcttt
    10381 gttttcagtc tgacatcttc ttcaaacgag ttggtacata tagtttaaca aagagcatag
    10441 taacaatcaa ataagccgct agcggaaccc atcgctgctg cccatctaat acgactgcag
    10501 caatcaaacc aaaaataacc gacgcgataa ttaataccca tgtgagtaga tactgaatcg
    10561 cctgcttctt ctgattctta tggattggat aaagctgaac gagaaccata tagcggaact
    10621 ggttcgctaa aggtaacagt tggaaaccga tcaaatataa aaataagaaa ccgataatca
    10681 gactaaaata aaagtcgact actccggcaa tcaataaccc acctataatt actaatcgga
    10741 aataaagacc actaaaatca gaacctcttg ccaatcttct tgcaaacaag taaagataag
    10801 tgttttttga ttctctttta atatgagtca atataggatc caaatagcgt cttcttttga
    10861 tctttgccgt aatttcagga acatctgtga ataaattgat aaattgatag atctgatgca
    10921 acctatgttc ttctacatgc accattttct cccaatccaa tcctttttct tgttgcttcc
    10981 acaaaaacca gtaatataga cttgcctgta ctaatgcacc tgctattcct gcccatatta
    11041 gccaataaaa tgatagaaac aaaaagatca gagaagaaaa aaaccagcct aatttccaat
    11101 aacgaataac ttgttgatct agtcgaaaca attctgctct cttgatttgc agatgactga
    11161 atttcaaact ccataatatg atcagataga cgaaaaacat cgaaaaggat tgctccgtac
    11221 taaccaccac taatggcatg gccaatccgc atgccaatat caaaaagatc aacggaaaaa
    11281 tgcaagaata gcgaaaagct cgttccaaat aggtttgcat ttctttttct ttaggaagta
    11341 aaaaaacaat atctgctgcc tctgcaaaag tagccatctt acccacgaat aaacagagca
    11401 tccaaaatac agagattatg agccctccaa ttgcaaacgg tggctgcagg gttttcaacc
    11461 aattagaata atataatccc acaccgccaa tcaagaaaag acaaactaaa acaaaatgat
    11521 cgttcaatac ataacgcata tatttcatca ttttcttttg atgtcttgct aaacgttttt
    11581 gaaaaaaact actcatgtgc ctgctccttt gttaaagcaa tatagatttc gtccaaagaa
    11641 gaatccggca gctgaaattc ttttctcaac atctcaagag aaccgtctgc tcgtatctct
    11701 ccgttatgta agaccacaaa acgatcacaa tatttttctg cagtagctaa aatatgcgta
    11761 gacatcaaaa taccggctcc ttcttttttc atttctacca tcaattcaag caatgcattg
    11821 atagctagtg gatccaagcc taaaaatggt tcgtcgatga tatataaaga aggctcgatc
    11881 aaaaaggcgc ataagatcat cactttctgt ttcattccct ttgaaaaatt agctgggaac
    11941 cagtcaagac gattagataa ccgaaaagtc tccaacaact tttctgctct tttcattgct
    12001 atatccatag gtatatcgta ggccatcgca gtgacttcga tatgttctct caatgtcaat
    12061 tcttcatata aagaaggagt ctctggaata tacccaattt gtttgcgata ttcttccgta
    12121 tttttcttta aggatagatg attgatttct atcgttcctt ttgaagcttc cagcagaccg
    12181 ataatgtgct tgatggtcgt acttttccct gctccgttca gtccaatcaa ccctactatc
    12241 tctccaggct gtacatcgaa actgatatct tttaacacag ggagatgtcc gtacccccct
    12301 gtgacatgat cgataattaa actcattctt tttcctccgc catttatttt tatttattat
    12361 accacgaaga aaatgacggt attttgtttt tttccttaca atagctttaa cttatggtaa
    12421 agttaagctg aagtatttat tctttataga aaggatgttt tatatggaaa actgtatttt
    12481 ttgcaaaatc atcaatcaag aaatcccaag ctataaaata tatgaagacg acaaggtcta
    12541 tgctttttta gacatctctc aagcaacgaa aggacacaca ttagttgtgc ctaaacgtca
    12601 tgtagcagat atttttgaat atgatcccga acttgcagga gaggtttttt cgcgtgtgcc
    12661 taagattgct caagcattgg aaaaagcttt tccagaaatg aaaggtctta atatcttgaa
    12721 taacaaccgt gaactagcct atcaatctgt ttttcattca catatccatt tagtccctcg
    12781 ctacagcaaa gaggatgatt tttctattca ctttgtcaat catcaagata gttatggatc
    12841 ggaagaacta aaagctattc aagaaacaat cgtaaagcag gtgtcctgcg atgattaaaa
    12901 actttttcaa aggactgtta tttggagctg ctgctggcac tgtcggaggt ctgttattgg
    12961 cgcccaggag cgggaatgaa acaagaaaga aactgatcga agaattggaa gaagcaacaa
    13021 ctctgacaaa tgatttaaat aacagcctga atcattttaa aagcgcattg atcgaaacaa
    13081 aagaaacagc tgaatctgtg attcctgctt tccaagaagc aatcaaaaaa gatatcgaag
    13141 attttaaatt ccaagccgaa ccacgtatca cacaaatcaa tgaacaagta gaaaaactaa
    13201 aagctgatct gccagagtat cctgaacaaa tataataatg attattttct caacagattt
    13261 aactatataa gtgagaaaag tttgcgtatt tcctaggaat gtgatgaaat tttgaatttt
    13321 gtttgaaact accacgacaa cgtttttaaa ggaaatggga tatggtatag ttaatatata
    13381 aaaataataa ctaattacag gagtgcaaat tttaatgaaa aaaaaatcaa ttattttggc
    13441 agctactagt gcgttggctg ttttaactct agcagcttgt tcaggcgata ctaataaaga
    13501 tatcgcaacg atgaaaggcg gaacgatcac tgtatctgat ttctatgatg aagcaaaact
    13561 tgaatcatcg aaccaatcct tagtacaacg gatgatcatt tataaagtat tcaataacaa
    13621 atatggcgat aaagtaaccg ataagcaagt ggatgcagaa tacgacaaac aagcgaaatc
    13681 attaggtgat acttttgaat cacaattaga agctgctgga tatacgaaag atacttataa
    13741 agaatatatt cgtaacaatt tagcttttga agctggcttg aaagctcacg tagatattac
    13801 agatgacgat ttgaaaactg cttggaaatc tttccatcca gaagtagaag cacaaatcat
    13861 caaattatca agtgaagatg aagcaaaaga tgtgaaaaaa tcagcggatg atggagatga
    13921 tttctctaaa ttagccaaag acaaatcaac tgatacagag acaaaagaag atggcggtaa
    13981 agttaaattc gactcaacga caacaactat tcctgcagaa gtaaaagaag cagctttcaa
    14041 attgaaagac ggtgagatct ccgatgttat tactacaaca aatcctactt cttatgcgac
    14101 agaatactac gttgtcaaaa tggtgaaaaa ccaaaataaa ggcaatgaca tggataaata
    14161 caaagatcaa ctaaaagata tcgctactga aactaaacta agtgacaatg cattcacaac
    14221 taaagtaatc ggtgaagaat taaaagatgc aaatgtaaaa atcaaagatg atgcctttga
    14281 aaatgtgttg agtgcattca caacaacttc atcaagtacg aaagactcaa gtgaaactac
    14341 agcttcaaca aagagttctg acacaaaatc aactgattca acaaaagaat cttcaactga
    14401 agaaacaact gattcatcaa aataaa
//
LOCUS       DAZBTF010000068        14139 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000068 DAZBTF010000000
VERSION     DAZBTF010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.068"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(51..3704)
                     /gene="rpoC"
                     /locus_tag="ACHGNS_002211"
     CDS             complement(51..3704)
                     /gene="rpoC"
                     /locus_tag="ACHGNS_002211"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFN6938134.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3783..7400)
                     /gene="rpoB"
                     /locus_tag="ACHGNS_002212"
     CDS             complement(3783..7400)
                     /gene="rpoB"
                     /locus_tag="ACHGNS_002212"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFN6938135.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8128..9096
                     /locus_tag="ACHGNS_002213"
     CDS             8128..9096
                     /locus_tag="ACHGNS_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN6938136.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9114..10019
                     /locus_tag="ACHGNS_002214"
     CDS             9114..10019
                     /locus_tag="ACHGNS_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6938137.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10016..10822
                     /locus_tag="ACHGNS_002215"
     CDS             10016..10822
                     /locus_tag="ACHGNS_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6938138.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10979..11947
                     /locus_tag="ACHGNS_002216"
     CDS             10979..11947
                     /locus_tag="ACHGNS_002216"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFN6938139.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12264..12788)
                     /locus_tag="ACHGNS_002217"
     CDS             complement(12264..12788)
                     /locus_tag="ACHGNS_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFN6938140.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13075..>14139
                     /gene="scm"
                     /locus_tag="ACHGNS_002218"
     CDS             13075..>14139
                     /gene="scm"
                     /locus_tag="ACHGNS_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFN6938141.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         4003 a         3047 c         2793 g         4296 t
ORIGIN      
        1 caaaaaagag gttgtgacaa taatttgtca caacctcttt tttaaaatat ctattttgtt
       61 tgattgttca ttgcgtcttc tgcagccatt tgcgcttcaa tatctgaaat actgtagaca
      121 ttttcgcttg ctacaccgac ttcttttggt tccatgtcac gataacgttg cataccagta
      181 ccagctggga tgatcttacc tataataaca ttttctttca aaccaagaag aggatctttc
      241 ttaccgcgga tcgcagcatc cgtcaagaca cgagttgttt cttggaatga tgcagcagat
      301 aagaaactat ttgtttctaa tgatgctttt gtgataccta gtaagacagg tcgagaagtt
      361 gcaggaacgc ctcctgagac tagcgtattg tagttttgat ctttaaagtc tgcgatgtct
      421 aataatgtac ctggtaagat atctgtatca cctggatcca ttacacgaac tttacgcagc
      481 atttgacgaa ccattacttc gatatgcttg tcgccgattt ctaccccttg catacggtaa
      541 acacgttgta cttcacgaag caagtagttt tcaacagata atacatcgcg aacttgcaac
      601 aattgttttg gatcgattga tccttctgtc aatggtgcac cacggtggat catatcgcct
      661 tctgctactt tcatacgtgc tgtataagga acagtatacg tacgtgtatc tgtttttcct
      721 ttgattgtta cttctttggt acgtgttgca ggatcttctg agatatcgat gacatcccct
      781 gttacttctg tgatcactgc ttgccctttc ggattacgtg cttcaaagat ttcttggaca
      841 cgaggaagac cttgagtgat atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc
      901 gtcaactgag taccaggttc accgattgat tgagcggcga tcgtaccaac tgcttctccg
      961 acttcaacgt cagagccagt tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt
     1021 tttgtattac atgtgaagac agaacggatt gtcactgttt cgacacctgc atcaacgatt
     1081 tgttttgcga tgtcttcgga aatcaattgg tttcctgcaa tgatcaattc atttgtttca
     1141 ggatgacgta cttctttacg ggtgtaacgt cccactaaac gatcttctaa tgattcgatg
     1201 atttcatttc cctcgcggat ggcttggatt tcaagacctc ggtcagtacc gcaatcttct
     1261 tcacggatga ttacatcttg ggccacgtca accagacgac gagtcaagta acctgaatcg
     1321 gcagtcttca aggctgtatc ggtcatccct ttacgagccc cgtgagttga gataaacatt
     1381 tccaagacgg atagtccttc acggaagttc gaaatgattg gcaattccat gattcgacca
     1441 ttaggagcgg ccatcaaacc acgcattccg gcaagctgtg taaagttgga aatgttacca
     1501 cgagctccag aatcagacat catgaagatt ggatttttcg cttccaaacc ttccatcagt
     1561 ttttgttgga tgctgtcttt tgcagcattc caaacagcaa tgacacgttc ataacgttcg
     1621 tcatctgtga tcagaccacg acggaattgt tttgtaatag tttcaacttg tttatgggca
     1681 ttttcaatga tttcttgttt ttcattcagt acactgatgt cagcgatccc tactgtgata
     1741 ccggcatgtg tcgaatgttt gtaccctaag tctttcatac ggtcaagcat cttagaagtt
     1801 tctgttactt tgaatcgttt gaagacttct gcgatgatgt ttcctaagtt tttcttcttg
     1861 aacggtaata ctaattcttg ctctttgata tgtgcaggaa tatcagtacc agcttcaaca
     1921 aagtatttgt ccggtgtttg gacggtcaag ttgaagtctg ttggttcatt cagataaggg
     1981 aattcaggag gcatgatctc gttgaagatg atcttaccaa ctgttgtgat catcgtacgt
     2041 tctttttgcc attcagtaaa tggtttttca cctaatgagt tagggttcac accgatacgt
     2101 gaatgtaaat gaacatatcc gttgcgccat gcgataacgg cttcgttttg atctctgaag
     2161 accattcctt cgccttcgcg tccttcttct tccattgtca agtagtagtt acctagaacc
     2221 atatcttggg aaggagtaac aactggttta ccatctttag ggttcaagat gttttgagca
     2281 gctaacatca acatacgtgc ttctgcttgt gcttcttcat tcaatggaac gtggacagcc
     2341 atttggtctc catcgaaatc ggcattataa gcttcacata caagtggatg caaacggatt
     2401 gcgcgtcctt gtactaaaac tggttcgaat gcttggatac caagtcggtg aagcgtaggt
     2461 gcccggttca gcaatactgg gtgttccttg attacgtctt ctaaaacatc ccatacttcg
     2521 tcttcttgac gttcgatttt acgtttcgca tttttgatgt ttgaagctag ttcgcgttga
     2581 acaagctcac gcatcacgaa tggtttgaat aattcaatgg ccatttcttt tggaagtcca
     2641 cattggtaca tcttcaagaa aggtccaaca acgataacgg aacgacctga gtagtcgaca
     2701 cgtttaccta gcaagttttg acggaaacga ccttgtttcc ctttcaacat atgagaaaga
     2761 gatttcaatg ggcggttacc tggtccagta actggacgtc cgcgacgacc gttatcgatc
     2821 aatgcatcta ctgcttcttg cagcatacgt ttttcatttt gaacgatgat tccaggtgca
     2881 ttcaagtcta acaaacgttt caaacggtta ttacggttga ttacacgacg gtaaagatca
     2941 ttcaagtcac ttgttgcaaa acgtccacct tctaattgaa ccattgggcg taaatctggt
     3001 ggaataacag ggataacgtc cataaccatc cagcttggtt gatttcctga agcgcggaat
     3061 gcttctaaaa tatccaaacg gcggattgca cgtgtacgtt tttgtccttg tgcagatttc
     3121 aattcttctt tcaattcggc tgcttctgca tctagatcaa cattatcaag taattgttta
     3181 atcgcttctg cacccatagc agcttggaat tcttgtccat attgttcacg tttttcgcga
     3241 tattcgcgtt ctgtcaacaa ttgttttttc tctaaattcg tatttcctgg ttcgatgact
     3301 acatatgaag caaaataaat gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga
     3361 cccatacgtg aaggaatccc tttaaagtac cagatatgtg aaacaggagc tgctaattca
     3421 atatgtccca tgcgttcacg gcgaactttc gaacgagtaa cttccactcc gcatcgatca
     3481 caaacgattc ctttataacg gatacgtttg tattttccgc aggcacattc ccagtctttt
     3541 gtaggaccaa aaatccgctc acagaacaac ccttcgcgtt caggttttaa ggtacgatag
     3601 ttaatcgtct caggtttttt tacttcaccg taagaccagc ttctgatttt ttcgggagaa
     3661 gccaaaccta tttgcatact ttcgaattta tttacatcga tcaaaagggg ttccctccat
     3721 ttcatttaat ggcaaaccgc aagacggctg atcttcgacc gccttgcagc tcatagaccg
     3781 gattagtcta aattatcttc tgcggtttca aagttttgga tgacatcgtc tttttcatct
     3841 tccacttgtt cagcagcttt cttttctaat tctttggcag tttgctgttc ggcaaatttt
     3901 gtcaaagcat caacagtgat caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc
     3961 tctgtttctt cgatatccaa gacacgcatg tccagaccta gtgattgcaa ttctttcacc
     4021 aatacgcgga atgattccgg tacacctggt tttggaattg gttcaccctt gacgattgct
     4081 tcgtatgttt tcacacgtcc gactacgtca tctgatttgt atgtcaagat ttcttgtaaa
     4141 gtataagccg caccgtaagc ttccagtgcc catacttcca tttcaccaaa acgttgtcca
     4201 ccaaattgag ctttacctcc caatggttgt tgtgtaacca atgagtaagg tccgattgaa
     4261 cgtgcatgca atttgtcatc gaccatgtga gcaagtttga tcatgtacat cacaccgaca
     4321 gacacacgtc catcaaatgg ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg
     4381 ctggccatac cggcttcacg aactgtttcc catacgtctt catcacttgc accatcaaat
     4441 actggcgttg ctacgtggat acctaactga cgtgcagcca tacccaagtg taattccaat
     4501 acttgcccga tgttcatacg tgaaggcacc cctaaaggat tcagcatgat atcgattggt
     4561 gttccatcag gtaggaatgg catatcttct tccggcataa tacgggatac aaccccttta
     4621 tttccgtgac gaccggccat tttatcccct tcatggattt tacgtttttg aacgatataa
     4681 acacgaacta acatgtttac gcctggtgac aattcatctc cagcttcacg agtaaagatt
     4741 ttcacatcgt gaacgatacc gccgccgcca tgtggtacac gtaatgatgt atcgcggact
     4801 tcacgagctt tttcgccaaa gatcgcatgc aatagacgtt cttctgcaga taattctgtt
     4861 accccttttg gcgtgacttt ccctacaagc aaatcgccat cttggacttc tgcaccaata
     4921 cggatgatac ccatttcgtc taaatctttt aatgcgtctt ccccgacatt agggatttcg
     4981 cgtgtgattt cttcaggtcc taattttgta tcgcgggctt ctgattcata ttcttcaata
     5041 tggatagaag tgtatacgtc atcttttacc aaacgacggc tcatgatgat cgcatcttca
     5101 tagttgtacc cttcccaagt catgaaacca actaaaacgt tttggcctag agccatttcg
     5161 ccttgttcca tagatggtcc gtctgccaga gtatcccctt tttcgacttt ctcacccaaa
     5221 tgaacgattg gacgttggtt gtagcttgtt ccggagtttg aacgacggaa ttttgttaca
     5281 tcgtatttat ctaatgcacc gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat
     5341 tcaacgacac cgtcatgttt acataacaaa gcagctcctg agtcatgcgc tgatttgtat
     5401 tccataccag taccaaccca tggtgcttga ggattgatca acggcactgc ttgacgctgc
     5461 atgttggcac ccatcaaggc acggttggag tcatcgtttt ccaagaaagg aatacatgct
     5521 gtcgcaacgg caactacttg tttaggtgat acgtccatgt agtctacttt atcaatagat
     5581 acttctaagt tttcactttg agcacgagcc ataacaacgt cttgagcaaa tgttccatct
     5641 tcattcaatg ggctgtttgc ttgggctacg atgtaatggt cttcgatatc agctgtcaag
     5701 tagtcgattt ggtcagtaac acgacctgtt tcacggtcta cacgacgata aggtgtttcg
     5761 ataaatccga atttatttac tttcgcataa ctggacaaac tattgatcaa cccgatgttt
     5821 gggccttcag gagtttcgat cggacacata cggccatagt gggagtagtg aacgtcacga
     5881 acttcataac cggcgcggtc acgagtcaat ccgccaggtc ctaaggctga aagacgacgt
     5941 ttgtgtgtca attcgccaag agggtttgtt tgatccatga actgtgacaa ctgagaagaa
     6001 ccaaagaatt ctttgatact tgccacaact ggacggatat tgatcaattg ttgaggtgtc
     6061 aatgtttcag tatcttggat cgacattctt tcacgaacca cacgttccat acgtgctagc
     6121 ccgatacgga attggttttg taaaagttca ccgactgaac ggatacgacg atttcctaag
     6181 tggtcgatat catcgacatt gccgatacct tccatcaagt tcaagaaata gctcattgaa
     6241 gcaatgatgt cggctggacg aaccgttttt acagctgctt ctggatagcc gtttccgatc
     6301 acgtttactt cacgttcagg gtctcttggt gaaaatactt tgatgacttg gacagtcatt
     6361 ggatctgtga ccacaccgtc ttctgatgga tagtaagtca ctgaatttag gccattatca
     6421 atgaaaggag ctagtgtttc catgacttgg tgagtcagaa ctgttccttt ttcaacgatg
     6481 atctcgcctg tttctggatc aactaatgtt tcagctaatg tcagattcaa tagacgtgtt
     6541 ttcaaatcta attttttgtt tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt
     6601 ggatcgaaga aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct
     6661 ggacgcaaac gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt
     6721 ttatgcaagt ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag
     6781 atcgtatcat cggaaccaaa tcctaacgca cgaactaata cggttaaagg aatcttacgt
     6841 gtacggtcga tacgtacata agagatgtct tttgcatcag tttccatttc tagccaagct
     6901 ccacggttag ggataacagt agaaccgaat ccttcttttc cgtttttgtc gacttttcca
     6961 tggaaataga cacctggtga acggactaac tgagaaacga ttactcgctc tgccccgttg
     7021 atgatgaatg ttccttgttc tgtcattaat gggaaatcac cgaagaatac ttcttgtgct
     7081 ttgatttcgc cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag
     7141 tttgcatcat gcgcacgagc ttctgctact gtatactttg gttctttcaa ttcataatct
     7201 acaaattcca aagatagatt gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt
     7261 tctctaagtc cctcatctaa gaaccattgg taagagtctg tttgaatttc gatcaaattt
     7321 ggtaattcca acacttcact gattcttgcg aaacttctac gttcgcgatg ttttccgtat
     7381 tttactacgt gtccagccaa gctcttcacc cctctaaaat gtcattcaaa aagactaccc
     7441 gatcaattgt tacaataata tttcaaatat tatttttagg taacaacaga cattttttgc
     7501 ggcttttagg caaaaaaaaa ccaaaaatag aacattctct tgaaacctta cttcgtgagc
     7561 ttctactttt gtttccttat attaaagccg aaacggacaa tatttcgttt ctgccttttc
     7621 ttcatcagat agtttttgca acttcatata ctacacttat tttctataaa agtcaacact
     7681 tcctggcgtt tcttctgctt tttcgcaaca ttttcttaat gttttcaatt ttatgttttc
     7741 aattatctga caattcttaa ttgacagacc tttttatctc ggttatactt tagaaaattc
     7801 attttgtgga ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa
     7861 taacttttac tttagctact ttagataggt ttgtattcat catgatggat acaaacgtta
     7921 aacgacagtt tgtatctatg atggctaaag aactttgtat tgcagcaaaa agccacgtag
     7981 acaaaatgct aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag cattctactt
     8041 tttcaattta ttttccgact tttcaatcca tttggacatt ttgtctgaat ggattttttg
     8101 tttcccaaaa ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg attaggcgct
     8161 gccctgctac tagctttagg aggttgcgca caaaaagaca attccgcaga ggcatcagat
     8221 ggacaaaaaa caattggcgt attgcaactt gtacaacata actcgttaga tgctgcctat
     8281 aaaggattca aagaaggatt agcagaagaa gggtataaag aaggcgataa tctaacaatc
     8341 gattatcaaa atgcccaaaa caatcaagac aacctgaaaa gtatgagtga gaaactcgtc
     8401 aaagaagatc ctgatttgct acttggaatc gccactccag cagcacaatc tttattaaat
     8461 gaaacaacca ctatcccgat gactgtcaca gcggtcacag acttggaaca agcccattta
     8521 gtcacatcca atgaaaaacc tggcggcaat gtgactggca cgacagatat ggtgccaatc
     8581 gaaaaacaaa tcggcttact gttaaatatc gtgccagacg ccaaaacaat cggcatcatg
     8641 tataacaacg gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa agaattgaaa
     8701 aaagcgggcg tcaaagtcaa agtactgaca gcaaattcca caaatgatgt ccaacaagtg
     8761 acgaaaagtc tagctaaaga tgtagatgga atctatattc ctactgataa cacgttcgca
     8821 agtgcggcaa gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc
     8881 gcagttgatc aagtcaaaga cggcggtcta gcaacagtag gaatcgacta cgaaagcttg
     8941 ggtaaacaaa cagggatcat ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt
     9001 cctgtcgaaa cagcgaatga tttggaactg tatgtcaata aagacatggc caaagcatta
     9061 ggcatcaatc cagacagcat caaagaagct gaataaaaca caggaggatt ctcatgacaa
     9121 atttgctctc tcttttctta tcatctgcat cgcaaggggt cttatgggcc ctacttgcga
     9181 ttggtgtata cgtcactttc cgtatcttgg acattgccga cttgactgct gaaggaagct
     9241 ttcctttagg tgcagggatc gcggcagtaa gtattacaaa tggctatcat ccattgacag
     9301 cttgtcttct aggcttttta gggggtgcgg cagcagggct tgtttccgga ctgcttcaca
     9361 caaaattgaa aattcccgcc cttcttgcag ggatcatcac catgactggt ttatattctg
     9421 tcacatcccg tgtgatgggt gcgccgaaca tctctttatt gggtcagcca acgatctttc
     9481 attgggtcga gtcattcggc ataacaaaag acaacgctgt actgatcgtc ggtctgcttt
     9541 ttgctttgat cgttgtatgt ttacttgttt tgttcttacg tacagaaaca ggacttgcta
     9601 ttcgtgcaac tggcgataat ctggcaatga gcgaagcaaa cgggatcaac acagataata
     9661 tgaagatcat cggttatatg atttctaatg gatgcattgc tctttctggc tccttgcttg
     9721 cgcaaaacaa cggctttgct gatttaaatt ctggtattgg aacgatcgta attggacttg
     9781 cttctattat catcgcagaa gttttgttac gtaaccagcc actatttctc cgtctcttga
     9841 ctgtcgtttt aggtgcggtc atttatcgtt ttattttagc gttagtcttc gaactgaatg
     9901 ttgaaccaag tgattcgaaa cttgcttctg ctttcgtgct agtgatctgt ctttctttcc
     9961 cgcagatcca gcaaaaattc catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa
    10021 tgacgtctta acattggaaa atctccatca tacctttgaa aaaggaacga tcaacgaaaa
    10081 tcatgtcctg aaaggcatta atctttcctt agatcccggc gacttcgtga cgatcatcgg
    10141 tggaaatggg gctggtaaat ctactttgct gaacagcatc gccggtacat tttctgtcga
    10201 tcagggtcaa attttattga atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc
    10261 gaaatccatc agtcgcgtat tccaagatcc taaactaggg acagccgttc gtttaacggt
    10321 agaagaaaat cttgctcttg ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt
    10381 caaaccacaa gaccgaacat ttttcaaaga ccagctggtt cggctaaatt tggggttgga
    10441 aaatcgttta tcctcagaaa tcggtttgct gtctggagga caacgtcaag ctattacgtt
    10501 attgatggcc acattacagc gtccagaact gatcttatta gatgaacaca ctgcagcgct
    10561 tgaccctgct acttccaaaa cggtgatgga attgacagaa aagctgatca ctgagcagaa
    10621 tctcactgct tttatggtca ctcatgacat ggaagacgcg atccgctatg ggagtcgact
    10681 gatcatgctg catcaaggaa ggatcgtcgt agatatccag caagaggaaa aacagcagct
    10741 cacagtgccc caattattag cgttgttcaa acaaaacagc ggcacggcat taaaagacga
    10801 tcaagtactt ttatcttcat aatcagaaag aggatgggac aaaaaatgaa cgcccaccct
    10861 cttctttttt ctctttcctt ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga
    10921 aaactatttt gttctctcct tcttcctgat agaatggatg ggaaaggagc ttgatgccat
    10981 gacaacgaaa gacaaggtac ttcaattatt gaaaaagaca ccagattttc tttccggaga
    11041 aaagttggca cagcaactgg aagtttcccg gacaagtatt tggaaagcca tcaaagaatt
    11101 ggaaaaggca ggctatcgtt ttgagcacgg gcctacaggg tatcgttacc tgccctctga
    11161 cgtactagat gcaaaagaaa tccagcaaga gctacggtcg tttattcctg atttgaaagt
    11221 acaaacgatg ttgacttctg aatctacgat gaaagatgtc aaactggcag cagctcaagg
    11281 agattctgtt tcgaagttat ttgttgctga tacacaagaa caagctcgtg gacgttttgg
    11341 acggcctttt ttcgctgaac ctggacgagg aatctacatg agtctcttgc tccatccaga
    11401 caaacggttt gatgaattgc ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc
    11461 cattgaatct gtaaccggtc gttctgcttc gatcaaatgg gtcaatgata tctacatgga
    11521 tggaaaaaaa gtctgtggga ttttatctga agcagtcagt gatatggaaa gcggcaggat
    11581 cagtcatatc atcattggga tcgggcttaa tttttctgtt cctcaaaatc attttccagc
    11641 tgatctccaa gaaaaagcca tctctgtctt tcccgacgga caagctagtg tcacaaggaa
    11701 tcagctaatc agcaaaatct ggcaagaatt gttcacttat ctttctcacc cagaaaaagc
    11761 gttggacttc tataaagaaa aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa
    11821 aggaacgact tatgtcggtc ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca
    11881 aacaaatgaa cagaagatga gtttgtcttc tggcgaaatc agtctcacat ctatccgtac
    11941 gcaataatta tcagatttca caaaaggaca cctgatctca tacagtgtcc ttttttctga
    12001 attctcttca aaagactctc cttattctat cagctgacag tttctctatc ctctagaaaa
    12061 aggtattcta aaaatatcta taaagcatgg tacactttct ttaaatgaat caatgcaaaa
    12121 aacaagagag tcatcagttt acacgatcaa aagagatagc aggccgcttc ctttttcaat
    12181 cttatagata gaccatgggt aacgataaaa acaaaaaaga acttgcgact tattttgccg
    12241 taagttcttt tttagctagt gccttaatct cttaatccca acaaacgtct cgcatagatc
    12301 acctgaccag agtgcatagc agcatcatca ataatagaaa cgagacgcat tcctcgtgtg
    12361 actggtggtg tccagttctc gtcaattact tcatctagct cggaaggttc cagccttctt
    12421 aagtaagaaa tagcaaaatc agcagctcct cgcaagtatc ctagcagatc ttctatttga
    12481 ttagcttgga tctgctttgc ttgttccaga ctatgcttcc aatcttgttc gttatctgct
    12541 atcgtcatcg gaaaccggct ttcccagtct tgactatgcc aaattggaga agttcctgct
    12601 aaatcagcaa tctggaaatc taattctcga gcggtatgcc aagccagcca agtcattgat
    12661 ttgatctgcg gggctctatc ttccactgga aaacgattgg cctcctcaat agagacgcct
    12721 tcaaacaaac gttcaaaccg atcgtatgcc cgatccacac tctcgcatag catatctaaa
    12781 taatccattt attttcctcc tttttattta tcctacttac tttttcctaa ttctaggata
    12841 cctacttata tgttactaca cacccttatt ttttacactt taacagtcac catcatcaat
    12901 agtttggaga ttgaaagaaa aagatagtgt cgaactatca attgaacaag aagcaacaat
    12961 atgaacgttt cattaatatt ctttaaataa acaatgattt ttcaaacgaa cagtttaaga
    13021 ctaggagaaa aagagtataa taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa
    13081 aaaagggcaa gtttactagt cctagttgct ttgttgttca atatcttttc gtatggtgtg
    13141 gccgtttcag cggaaacata cattcctaaa gaccctggga atatttataa tgatctaact
    13201 ggtaactatg gcttgttagg aattgctagt caattccacg tatttgctaa aaataagaca
    13261 actgtgaatg cacatacaga cggaaatata gcaacacatg aacttgatgc aaaaaataat
    13321 tttggtacag agcttcatga aggtttattg aaccaagaaa taaattatat acaaaaaact
    13381 gattctttcg ctaattcttc aggcatacct acaggtagca caagaatgaa taaatttgtc
    13441 gttggcagta acgtggatgt agcacttcaa gatggtcatc ctacagtcaa tggatccagg
    13501 cttgatcact taactccaga tgatttttat aaagatcgtg ctgggcatga atatattgat
    13561 ttcgagcagg aatttgctaa attgaatgta gcagcaaatg atcttgcaac gatcactcca
    13621 gctaaaacat atactgctgc agattttcca gacatgaaca atcggacaat cgatttaact
    13681 gatttatcag ataccgggtt tttattggta aatatcgatg cagaagtgtt aacgatgaat
    13741 acgccacttc aaatcattaa tccaaatgat caagtagttg tatttaatgt catcaacagt
    13801 tcttctgcat tgaacgtaca gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca
    13861 gaagattttt cggatgcgaa tatctcttgg aattttggaa atgagatgac tgatttgaca
    13921 atcagtgctc cttttcaagg aacaatcctt gcgcctaatg ctactatccg cattactcaa
    13981 aaccaagacg gaacgattat aggaacaaat gtgattttag atgcagctac caatcgttgg
    14041 gatcctaacg aaatatttat cagcgacaat ggtacagata cgacagacac aagcgacagt
    14101 acagatacat ctaccacaag tgatagctca gattcatca
//
LOCUS       DAZBTF010000069        14130 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000069 DAZBTF010000000
VERSION     DAZBTF010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.069"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..244)
                     /locus_tag="ACHGNS_002219"
     CDS             complement(<1..244)
                     /locus_tag="ACHGNS_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFN6938142.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELI"
     gene            complement(237..1829)
                     /locus_tag="ACHGNS_002220"
     CDS             complement(237..1829)
                     /locus_tag="ACHGNS_002220"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFN6938143.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHE"
     gene            complement(2339..3064)
                     /locus_tag="ACHGNS_002221"
     CDS             complement(2339..3064)
                     /locus_tag="ACHGNS_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938144.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(3218..3544)
                     /locus_tag="ACHGNS_002222"
     CDS             complement(3218..3544)
                     /locus_tag="ACHGNS_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938145.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(3581..3946)
                     /locus_tag="ACHGNS_002223"
     CDS             complement(3581..3946)
                     /locus_tag="ACHGNS_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFN6938146.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(3983..4198)
                     /locus_tag="ACHGNS_002224"
     CDS             complement(3983..4198)
                     /locus_tag="ACHGNS_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN6938147.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            4327..4662
                     /locus_tag="ACHGNS_002225"
     CDS             4327..4662
                     /locus_tag="ACHGNS_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN6938148.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(4738..6735)
                     /locus_tag="ACHGNS_002226"
     CDS             complement(4738..6735)
                     /locus_tag="ACHGNS_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938149.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(6833..7108)
                     /locus_tag="ACHGNS_002227"
     CDS             complement(6833..7108)
                     /locus_tag="ACHGNS_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6938150.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            7340..8074
                     /locus_tag="ACHGNS_002228"
     CDS             7340..8074
                     /locus_tag="ACHGNS_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN6938151.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            8113..9255
                     /locus_tag="ACHGNS_002229"
     CDS             8113..9255
                     /locus_tag="ACHGNS_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN6938152.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(9883..10368)
                     /locus_tag="ACHGNS_002230"
     CDS             complement(9883..10368)
                     /locus_tag="ACHGNS_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HFN6938153.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(10370..11041)
                     /locus_tag="ACHGNS_002231"
     CDS             complement(10370..11041)
                     /locus_tag="ACHGNS_002231"
                     /inference="COORDINATES: protein motif:HMM:NF037286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938154.1"
                     /translation="MSRFSINVVEVDDYSEITDILKSIRKKYLRKTIFISGSAVDYTP
                     FTSESKGLKFVEKLAYRLSESGYRIVSGYGLGIGNSIVSGVLKQRRNLRKNNIQDVLS
                     LRPLPLDMPHEWRRYRENIIAESGISIFVFGNKLESGEIVTADGMIEEFELSVQNENI
                     VVPIGFTKGASKVLFDKIQENFSDYFDDSLKSKFQALEKLDTEGEVDKKVEQIVSLIN
                     SIQED"
     gene            complement(11304..12497)
                     /locus_tag="ACHGNS_002232"
     CDS             complement(11304..12497)
                     /locus_tag="ACHGNS_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN6938155.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(12581..12781)
                     /locus_tag="ACHGNS_002233"
     CDS             complement(12581..12781)
                     /locus_tag="ACHGNS_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFN6938156.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            complement(13205..13438)
                     /locus_tag="ACHGNS_002234"
     CDS             complement(13205..13438)
                     /locus_tag="ACHGNS_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6938157.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(13445..13876)
                     /locus_tag="ACHGNS_002235"
     CDS             complement(13445..13876)
                     /locus_tag="ACHGNS_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFN6938158.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
BASE COUNT         4295 a         2895 c         2270 g         4670 t
ORIGIN      
        1 tgattaattc attatatttt ttttcagtaa ttttatatct tgtttcgtaa ttatcataaa
       61 tagcattttg ttgttcatag acaacaaaat tgctattaga tacaaagaat tctttcttta
      121 aggcactccc aaatggtccc ctccttactc catctttttt atcaagtaag cattcaaact
      181 tacgctgttc ccaagcttcc gtaaatcctg ggaaacgaat ttctggttgt atatcattac
      241 tcatgtttga acacctccag cgcaccttgt aaccacgctt cattttcagg tgttgtttgc
      301 agggaagaga tagcttctaa aagttctttt tctagcactt gctgttcttg tcgtatcttc
      361 ttgatgtcat ttccgacatg aaccatatca attggttctt cttcttcaaa cgtatctaca
      421 tagcgtggaa tatttaagtt atagtcattt tctttgattt catcaaagtt tgccaaatga
      481 gcgtatttct cgacatcttt tctttcagca taggtttcga ggattttttg aatgttttct
      541 tccgataatt tattctgatt ttttcctttg acaaattctc gactagcgtc aatgaataat
      601 acgtctcgag tttggcggtt ctttttcaat acaatcaccg tcgttggaat cgatgtcccg
      661 aagaacaagt tcgcaggcat accgatcaca gcataaatac tgccgtcttc tagtaatttt
      721 tgacgaatga ccccttctgc agcgccacgg aataaaactc cgtgtggcaa gacgatggcc
      781 atagtccctg tttccttcaa atgatagaaa ccatgaagaa gaaacgcaaa atctgctttg
      841 gattttggcg ctaattttcc ataacgatta aaccgagaat cgtctaagaa ggtcgtatct
      901 gctgaccaat tagccgaata aggtgggttc attactactg catcaaatgt gtaaggttca
      961 tctgttggcc aatccttatt caacgtatcc ccattgcgga gattcatttc ttcggcatct
     1021 accccatgta aaatcaagtt catttttgct agattgtatg tcgttgtatt taattcttgc
     1081 ccatgatatt taacgttatc tgggtgagtt aagtagttac gcacatttaa catcaatgaa
     1141 cctgaaccca tggttggatc aaaaacacta aagaatcgtc gttctttttg atccaacgtc
     1201 acgatttgtg ccatcatatc agaaaccata tgaggcgtgt aaaattctcc ggctttcttc
     1261 cctgcttctg aagcaaattg actgatcaga aattcataag catccccgat cacgtcccca
     1321 tcgtgaccta aaacttccac gtcattcaat ttcttaatga cttcggtaat cgtcacattt
     1381 cgttgttgct catctgttcc tagttttttt gattggagat ccacatcatc aaataaccca
     1441 ttaaattgat tgtaggtaga tgccaattgc acaaaggctt tgtttagatc attcaattgg
     1501 aacgttgctt gttttgcttg atccgctaag acgttgaaaa gatactctgg cgaaatcgca
     1561 taccccaaaa tatcaacgat tgttgcaatc agatcttctt ttgattcttc atctgaaagt
     1621 aattcgcgat acaacatcgt ttgtttgctt accgtatcat attcctctaa ggattcatct
     1681 gctaaaagaa ccacttgttc caacaaacgg tcggataaat atttatagaa aatcagtccc
     1741 aataaataat ttttatattc tgacgcatcc attttgctac ggagattatc tgccgcacta
     1801 aataattttt gatttaattc tgcacccaaa aagatacact cctatctctt atttaaagtt
     1861 gctatatacc ctcttatttt agcatactcc cttttagaaa aagcaaaaaa aaaaagaaag
     1921 aaacttattc ttgctaataa actacgcgaa aggagtctaa cgttgtttga tataacttag
     1981 actgatcgtt aacttatttt ttatatttct acatagttat ctttagaacc atttcagata
     2041 catatgaaac aattacatat gattttttca taaaaaaaca gtactgtcta gtagtataaa
     2101 atatacaaat ttcttaaaaa ttttttcact aaaatcacta cctgaaacag tctaaaacac
     2161 ttatatatca acgtttaatg aaactatctc cgtttcattt cagatacaaa taaatatagt
     2221 cgggctccaa atccgaaaaa aaataccccc agtagcttat cctaagctac tggagatgta
     2281 taagtacggt catataacaa cggggtaggg taaataaatg aaaaagatgg aagaatcatt
     2341 attcaataac ttttttaaat acttcgttgt aatcagttac ctgtccgttc ttaaatacca
     2401 cagaataatc ttttttccca ttttcaagta accgaagtgg aatatcttta ccaactttct
     2461 ctgtcactgc attactgata cctgttagat tagctaataa atctgataaa gattgagcat
     2521 ctgtagaaat ctctgtatca gtatttatcg tgattccttt atcttcattt acaagtttcc
     2581 aagtaggtgg tatagctgtg tatgtttcta caaatgaatt aagtttttca ttaagtgact
     2641 tctcttttat atttccatct aaaaactctg tatatatggc ttgagctata ctaaaacaaa
     2701 gctggccttg ttctaataat tttacatcat tagttagtaa agctgtgttc acgttatcaa
     2761 tcgcttcaag aagagtatct ttggtgtccc taacagcttt aggtgtgcct tctggaattt
     2821 ccctacttga tacctcgtcc attttatctt ccataaaccc cttaaaatca tatctttctc
     2881 ccaaatccat agaagttttt gagaaagctt tcatttctga gtctatttct tcaataatat
     2941 ttaacatgtc tgatacatat tgtttaggct gattcgtttt taatctatat gtggtaaata
     3001 tctcaattcc tccaattatc aataaaattg ccaaacaaat ccataataat tttttctttt
     3061 tcatttttcc tcctatacac tgctgttata acagtgacta atgtaaagat tttcccagca
     3121 atcatcagac tattatttac agggaatcaa aaaaactcct tagtaagctt acacctttag
     3181 gagttcctgt ttgaacggta tgcataatac atttaaatca atttaacatt aacacctcag
     3241 tatatggtat ctctacatat acacggtctt tttttggata gtactcgaca tttatattga
     3301 tagcacttcc attgtctaac gtagctttag cccggtacgt gttatcacca ttatcatgag
     3361 tgatcgtcac atcatcacat gtccgattta agttatactg ttcttgtaaa atctgagtta
     3421 ccaatggtac agatgacttc tctagtccag atgaacccgt cattcttgaa aaaattatac
     3481 ctataatagc caatacaacg atacctataa tgacaaactt cttattcata tttactttat
     3541 tcaacttttt atctcctatc tccaaaatga ttttctctca tcagtaactc attgacatcc
     3601 ccaatagtac accaaagatg gctccagcta cccctgctat catcaaaatc gtacccattt
     3661 gttcatcttt ctctcgatat aagtaaccca taataacagc aacagcacca aataccacag
     3721 gaataaatgc taatgatact accatagaaa tccaaccaat aatcaaatac agcttccctg
     3781 tctcttgtcc atgtacgatt tgttccttca tttcattaat tgtctgctca gtacttttct
     3841 ttactgtgtc tgttccggtt gtctcatctg tttcttgtaa tgctccgcaa aactggcaat
     3901 acttactttc atttgttatt tctttaccac attttctaca atacatgtct tctcctccta
     3961 aaatcattca attgcttatt agctaactgt ttactttatt aaatacatca gaaaacagct
     4021 catttattgg tacacttaat atctcagaaa gcattttcat ttgatagggt ttaggaatcg
     4081 ttctccctgt ttgccacgaa cttacacatt gctgtgtaac attcattttt ttggctaact
     4141 cagtttggga aagtcctttt tcttttctgt atcggctgag attacttact tttttcatga
     4201 ttctcccctc ctgttttatt atatacaaca atatgatgta tttcaactca taaaatatat
     4261 aaatacttca aatgtcatta caaatacaac aacttgttgt actctactga gaaggggggt
     4321 gtattaatga aacagaagca atatgcaaag gtcattagag aaaagcgaaa agcactcggg
     4381 tggacacaaa aagaattagc taaaaaaatc ttctctacac aacaggcagt agcaagatgg
     4441 gaaaattctg taacagaacc taacctcgat agcttaacag ctctttctag agccctagga
     4501 acccctgtaa gccattttct agacaatgtt gacatagatt atgaggaaga gtttttggct
     4561 ctctatcgct ctcttagtac tgaagatgca attcgaacaa ttgactatat gaagctatta
     4621 aaaaggcaag aaaatgaacg caatcagcta ttgaaagatt gaaaaataaa taacccaccg
     4681 aactttaacg gtgggtgtat tgagatgaaa taagattaag agctatggat aacctgtcta
     4741 gtttaatata attgggtctc ttctattcct tactctccgt ccatcatctc ttgttacact
     4801 tgtaaattga taaataggaa ctaattcaac tcctgataat atcaattctt ttattacttc
     4861 atctatccct gcccctttcg ctagatatac taaaaaacta ctgcttgctt tcacattgta
     4921 tagtttctta atatccttag aagctcgttc aagagtgaat ccttgtttaa caaagtagcc
     4981 tctgttttct attaaagcat ttttcacgct gttatccctg acatttcgta ggcgttcaca
     5041 aacagtgtta ataagttctt tttctgtcat tagatatgtc ccttgccacg ataagccctg
     5101 taaaacttca tgtgcttcat ctaaaatcaa ttgtttttcc ttgctaagtt ctgatcggga
     5161 attttgagga aatactcttt tagcctcttt ttcgttaaaa catatcttaa cccattcata
     5221 agaaaaatat tgatatgtaa aatgaaattt ttccagaagt ggaaggatat cttcattcaa
     5281 tttttgaatc acagaagttg cattaaaaac gtctaatcga tagacattgc ttctcttatg
     5341 tttaaagttt cctaacgctt tctcttcctt taacctattt aggtattcga gcatcttcaa
     5401 aggaacatct tcatcatcta atttttttat caatcctata aaagtcataa gtgatagtaa
     5461 cttattacat ttatctatgt cataagtttt accatagtaa gaactcattt ttttagctaa
     5521 ttgttccccc gacaaaatac aagtcacact ttccttacta tcatcaaaaa aattaacatc
     5581 agaaagcaag acatcataga cacgtcgcat ttcctctaga taattcctta ttgtcctgaa
     5641 aagtttgtct ttcattgatt caagagtgtc aaaaagtact tttgattcct ctttcaattt
     5701 tcgaaaatgt gtaggataca agtctaggtt ctctaatagc caattaaatg tttctacccg
     5761 actgtctttc tttaaaatta gcatcacaac tccaataaaa ttttcactga tatcacaatt
     5821 actggaatga caataaaaaa tggaatgccc agttttactg gtaaaaatac tagcagaggg
     5881 attatgatcg ttgtgaaaca gacatcttaa gtttttactt gacttaagta atttattcat
     5941 atcaatggtt aacaatcttt cataaatctc attaaaagac atgtcggagg aatcaaacag
     6001 gcagtcttta aaccactgtt tcatttcttc tttgtcgtca ttttttatca acttaacaaa
     6061 atcggtttca ttgactagtt catactctct ccctgaatca ctttgacaat ttttagaaca
     6121 tgtcatggta aaatcaatcg gaagctcagt aatatctaac atgttgtctt cattaattat
     6181 tatcactgca tctgacttat atcctccaaa aaacattcta ttcgaagcat ttacattgtc
     6241 atcacacccg gggatgttct ttttcaagta ttcgtaaaca gcaaccattt taccatagtc
     6301 agtaattgct tcttttaata gaaagacaat cttgtatttt tctagatttt cagagtaccg
     6361 aatacttttt tgtatcgcaa acgcagaatt atgaactaaa ttgatagata aaaccttttc
     6421 tatgcttaag tatctactat catctttact caaatctact accttcccct tctcatcttt
     6481 aacagtgtta tcgacatcca gaataattac tctggtttcc acaaaactct ccttgtcatt
     6541 gcctttattc tttgtcttgc ttggaataaa tgattgtgat gaatttagta tctctacaaa
     6601 ctcatctctt gtaacttctc tatagttatc taaacaccta tctcttacat cagttttttc
     6661 tttttttgta actggcttct ctaataattt ttctttatct acataaattg gaatattaat
     6721 cattgatgtt tccatttgtg tttcctcctt ataaaattct gttaaagact aaaacataca
     6781 gataataaaa acaaactctt tattactata tgttttagtc ttatttgttt actcaacctt
     6841 tataccgttc catgaaacta tcaatatcag attttttaaa tctaatgacc gtatcaattt
     6901 ttataacagg taacccgtat tgagaaacat attttctaat cgttcctact gatacaccta
     6961 gatattcact tgcctgctga tagtccatat actctagagt gactccagaa ataatgtctt
     7021 ctagcatagg ctttagcact gtaattatct ctttcacttg ttgttcaact tgtatagaaa
     7081 caatgctatc aatgatttgt agagacatat ttccccctcc tttcgtgatt gcttgaacaa
     7141 attttctaag aattatcttc gtattttttg ctcaaaataa aagtattttg ttattaaccg
     7201 attaatcaaa caaaaaatga ttaccagttc atcgacatac acataatacc tcgataaaaa
     7261 aggggtgtca atagttttgt aaaatttatt tacaacaccc cttttttgag gtatactaat
     7321 tttaggaggt gtggtcaata tgataaaacg tccaccaata aattatttag aacgaaaaaa
     7381 gatactcgga acaaaaataa aagctataag aaagagtaaa aaattaacac aaccagcatt
     7441 tggtcttatg attaacaacg gtcaattaat tgacaaaaag actatctacg aatgggagaa
     7501 gggaacctat ttaccaatac cagagcgatt atcccgaatt gctgatttgg gaaatatgag
     7561 tatagaggag ttagtttgtg gcaatgtaga agaatatatt ttgggaatta ttctttatag
     7621 agatagcatt gtactagatg gtataacatt tccagataaa aacctgttcc agcatcttcg
     7681 tcaacagttc ccaccagtac actctaattt agatacatgg ctggatagat attccaaatt
     7741 agaaccagag atgcaagaat ttattgctaa caagacttgt aataaagtta aaaatgaaaa
     7801 aattagtctt ttcaatatct taaaaataga agaattattc atcaatgcta ttgtagaaga
     7861 atttgacaat aacattcttt tcctcactag cagtatagaa gaactactag aaagaatggt
     7921 agatgaatgg ctacctattc aattgaaaga catgtcttat cccgaagaag ctgtgagaga
     7981 aattactgat aatataaaca agctagaaca aacgatttca tcaattggga aaaaatacac
     8041 taagaaaaaa atgaagggag gtgataccat ctagtggtaa gtactccttg taaactaaga
     8101 ggagatgatt tcatggcaaa aatatcaaag tacaaaaaga aaaacggtca agtggcgtgg
     8161 atgtataaag gacacattgc caccgatcca cgaactggtg aaaaaataaa tactacgaga
     8221 agaggtttta gtagcaaaca agaagctcaa aaagattatg acgagtacag acatcaaata
     8281 ttatacggtg ttaaaaagaa agcacctgat atgacatttg aagacctgta taatgaatgg
     8341 ataactcatc agcgtaccag tgttaaagct tcaacaatcg caatctcagt aagatacgca
     8401 aataatcaaa tacttccagc atttggtaag cttaaactat cgaatatctc tgtcccatat
     8461 tgtcaaaaag tggtagatga atggcacagt aaatatgaaa gttacgatta tatgagaaaa
     8521 cagactgcac aaattttacg ctacggggtt gcaatgcaat atattgacaa taatcctatg
     8581 gaaaaaacac ttcttccacg aaaaaaagag tatgagaaaa atcgtaaatt ttactcaaaa
     8641 gaggagctta ataatttact ggatgctttt aaagattttg gtaacatgaa acaatacgcc
     8701 ttcttcagat tattggcata cacaggtatg agaaagagtg aagtactcgc tttacagtgg
     8761 aaagacatag acacgtttaa taaagaacta catgtcaata aaactcttgc tgtagatgaa
     8821 tttggtaaag ttataattca atctccgaaa acaagagctt ctagacgagt tatctcttta
     8881 gatactgaga ctctttctat cctcaacaac tggaaattac agcaaaaaga agagtattta
     8941 aaacttggat acaatactag ctctaaagag caacatgttt ttacaacagt taaaaacact
     9001 ctatacattc caaacactgt taatgattgg ttaagatata ttttgaaaaa atataacttg
     9061 cctcgtatta cacctcatgg gtttagacat acacatgcta gtttattatt agaagcagga
     9121 gaatctgtca aggtagttca acaacgatta gggcatgaaa atagtaaagt gacattagac
     9181 atctatgcac atattacaaa taatgcacct aagaaaacag gtcaagactt tgctgatatg
     9241 atggctcatc agtaaaagag ctggtcaaaa tctggtcatt tctagtatac aggcaaaaca
     9301 aaaaagctag aacccttata tatcaagggt tctagctcct ataacaggtt tacccaacac
     9361 tgccttccaa tgactctttt gggaatcaga ggaaagaatc aggctttata gagatatttc
     9421 atctaacaaa ttactttaga ctgacagtat atatcagcag gtactgtcat cgggaacgta
     9481 gccaatatcg tatccaaaac agcaggtaga gaagctattt gaggattcaa gttattctat
     9541 ttcaaccatg aagttaagtt cattaaaaat aaagatagtt ggtatttcaa taatttttgt
     9601 atagagttaa tcactttatt gatgctaatt catcttgatt ggtattataa tcgtattcaa
     9661 gttgcgtggc gtgcttttgc tagaaaatat ataactgttc tttataaaga aatataagga
     9721 ttaaacattt tttatcatag agttttactt atctatagaa ttatgtctgt tcctaattca
     9781 gtttgatgaa ttaactgata aaaaaatatc tactatttaa agatacaggg ataagtaagt
     9841 aaaaaaataa catgtcatta gatagtagac agttccaact ctttatttac cttttttgta
     9901 tgctgcactg tcaatccaag ttcctaaatt gttgtatcca tcatcactta tataatcata
     9961 ttggccatca ataatttcgt caaaccacaa atattctcca tctggttttt gcccaattat
    10021 tttaattact gattgactag tagttgtcat ctggttaaaa tcttttattt tacctatatt
    10081 aacggccaaa atagcatttc ctcgcttata actctcctgg atttcatatt taacatattc
    10141 tcgatcaaga gtttctttac ctatcagcac cacagtcact gatgttccaa ttaattgatt
    10201 atctatccag ttttttatag caatatctcc attacgttta actttctcaa attcagcctt
    10261 atctacaaaa ccagcttcct ttgttcctcg caaagtccag ctgtttctaa ctaccattgc
    10321 tcttgttaca tctgcataat ggaaactaaa aaaaaccctt ctcaccatat taatcctcct
    10381 gaatactatt tattaaactg acaatttgtt caactttttt atctacttct ccttcagtat
    10441 ctaatttttc tagcgcttga aatttacttt ttaaggaatc atcaaaataa tcactgaaat
    10501 tctcttgaat cttatcaaac aaaaccttac ttgcaccttt ggtaaatcca atcggaacta
    10561 ctatattttc attttgaaca gataattcaa attcttcaat cattccatct gctgtaacta
    10621 tttcacctga ttcaagtttg tttccaaaaa caaaaatact aatcccagac tctgcaataa
    10681 tgttttctcg atatcttctc cactcatgag gcatatcaag gggaagtggt cttaaagaaa
    10741 gtacgtcttg tatgttattt tttcttaaat ttcttctctg ctttagaact cctgaaacaa
    10801 tactattacc aattccaagt ccgtaacctg atactattct ataaccggat tcagaaagcc
    10861 tatatgctaa tttctcgaca aattttaatc ctttcgattc agatgtaaat ggagtataat
    10921 caacagcact tcccgaaata aaaatagttt ttctcaaata cttttttcta atacttttca
    10981 aaatatcagt aatctcagaa taatcatcta cttcaacaac gtttatcgaa aatctggaca
    11041 aatcattgat ttgtagtctt ctttttgtca gttcataatt atatctctct tgatcaaaaa
    11101 aatcctcaga atcgacaaaa ttttccttta aaattatatg ggattttaga ttatcttttt
    11161 agaagttctt gaatgtcggt atagcccgtg tttccgggct tttccggcat ttttgatatt
    11221 gttagatact tacaaatgtt cttattacct ctcaccattt ccccctcaaa agtagtaaat
    11281 ggagtagtaa aaccggcctt gtgtcaggct gccagccgtt ccatttctgc ccttgcggaa
    11341 tcgaatgtgg cgtgtgcata atagttcagc gtcatggtga tattggaatg tcccataatg
    11401 tactgcaagg ctttggggtt cattcccgca ttggctaacc gggtgcagaa tgtatgacgc
    11461 atagcatggg gcgtcgtcgt tttcggcaac gcttcttcat ggctcttgtt gtacttcttc
    11521 acaagcctgc caaacatccc gccataatct accgccgtca tgggatagcc gttctgtttg
    11581 agaaacagga aattggcata cccgtcaata atgaacggct ttgcaccttt gcggtttttt
    11641 atcactcgct ggaacgcctg atacacttcc tcattcatgg ggatttttct gacgccgctt
    11701 tgggtcttgg gttcgtcaat atagtaaccg acgccggtat ttctcaaaag ctggtggctc
    11761 acgatgatta cccggttttc aaaatccaaa tctttgtccg tcagtccgca cagttccgaa
    11821 atgcgaagcc ctgtccccag cagaataacg attgcgtcat aatgcttttg gtaaacctta
    11881 tcaccttgaa taaaggacag aaaactttct tcctgtgctg gtgtaagagg cacctttggt
    11941 tccgtatcgt cgtccagaac gtcactaagc tggaagtcaa acgggttttt tcggatgcaa
    12001 tcgtcctgta tggcggtgta aaaagccgct ttcagggaac gcttgtcgtt attgatggtc
    12061 ttataagaaa ggcccttttc tttcatccga agcgcccatt cctttgcgtc ggacagcttc
    12121 acactgtcaa tcgggcagct ccccagccga tcttgttcca gaatacgcat cagccgttcc
    12181 cggcccagtt tggtgttgtg cttcacattg ccccggtgcc tgatctgctt ggcgtagagc
    12241 tggcagacgg tcattttctt gccgatggtg tctatcccgt cgtcaaggtc tttctggatt
    12301 tctttctcct tttcccggag tgaaatatcc tcccgtttac ctgccggggt cttatccgta
    12361 ggcactaact tccatgagta aacaaactgc ggttttccaa aggtatcaac atatttgtaa
    12421 gcatatcgtc cgtcttttct ctggctctct ccagaccgca aaacgcgatt tttgctgtct
    12481 cgtctttttt ctgacatctc gtcatgctcc tttccatgac ggaaagagcc gcatatatgc
    12541 ttataaatta ttataacata tctgcggccc tttttcacta ttagattgtg tccaaagtgt
    12601 caattatttt ctcaaactgc ttgcgtttga tctggatgcg gttgccattc ataatgaccc
    12661 agccagaggc cggattttcc tcggccagct tacgcaattt gttttctccg atacggaaat
    12721 atttagacgc ttcctcaatc gtcagcgtgt atttttccca aataggcaca tcatggttca
    12781 taatgcagcc ccctttcaca aattcaaagg tttgtgtgat ttaggggcat agacggacgg
    12841 ctgctggcac agctccacgg gactttcacc cccgcgtggt tctcacgcgg ccctacccat
    12901 tgcctgcgac gcttttaacg ctcggactgt ggctataagg gagtatcata gtcctgcctg
    12961 tacgtcgtcg cccgcaagcc gccacgctca cattgcggcg tggtgttcct cgctccgctt
    13021 ttcccgtagg gaaaagtatc atggcgcacc agccgtccgg ctcggcacag acagaatgtg
    13081 tccgtatgcc ctatgatgcg ccgccgttat cttgcgggca tatttatcaa ggtgcgatca
    13141 ggccaccggg aaaacaccga aacggggaaa gggtaagccg tttcatgctg ccggggccgg
    13201 ttcgtcagtt cgtgacgata gcgtggatga gcttgatttc cagacggcgc ttcatggcct
    13261 cgtccagaca cacatgcgga tagccgtttt cgtcatagag cgtccgggta cacagcttgt
    13321 tcatgtaacc gctgtaatac tgcaaaacgc gctctacggc tccggcatcc ccagcgcggg
    13381 cggcctcgat caccgaaagg ggcaaaagct caatgccctt gtagttcggg ttcttcatcc
    13441 gcgtctaacc tcccttcggc atcaacgcct ttagctgttt ccgcagctcc gataaggtat
    13501 ttgtcctgtg ccgctgaacg gcactccggg acattcccac aaggccgccg atctcaccat
    13561 ctgccatatc cagaacacag tgcaggatta aaatactctg ctccatgctg ggcagcgcgg
    13621 caaaggcttc ggcaacaagc tcattgttga tatgtaggtc atagccatgc gacgaaaacg
    13681 taaagctgtc gctggggtag tgatctactg tacagagctt gtccaaatct gcctgtgaca
    13741 aagagcttag tgacacttca cggtcacggc ggcgcttcat ttcagccaag tagcttttgg
    13801 cctcgttacg cagtaccgtc ttgcaaaaag catcgaacat gcaccgcgct tcatagtcgc
    13861 gggggatagc atccatcttc tcaccccctt tctgtggggg acttaggtgg ttctctccct
    13921 ttccgctaac aagactttga caaggccgcc agctacccgc taaatacctc ctttttgaaa
    13981 atttctcaaa aaattttttt ggggacagcc gggccgcaaa agacgacaaa aatcgcccgg
    14041 caggtcgcaa acctaccggg cgagaaaatg ctttgctatg ctgctgattt acatggtata
    14101 gtacatactc ataaccggaa tagccctgtg
//
LOCUS       DAZBTF010000070        13900 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000070 DAZBTF010000000
VERSION     DAZBTF010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.070"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(148..2223)
                     /gene="glyS"
                     /locus_tag="ACHGNS_002236"
     CDS             complement(148..2223)
                     /gene="glyS"
                     /locus_tag="ACHGNS_002236"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFN6938159.1"
                     /translation="MAKDLLLEIGLEEIPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFTHAKQYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2225..3142)
                     /gene="glyQ"
                     /locus_tag="ACHGNS_002237"
     CDS             complement(2225..3142)
                     /gene="glyQ"
                     /locus_tag="ACHGNS_002237"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFN6938160.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3526..4338)
                     /gene="recO"
                     /locus_tag="ACHGNS_002238"
     CDS             complement(3526..4338)
                     /gene="recO"
                     /locus_tag="ACHGNS_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFN6938161.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4481..5380)
                     /gene="era"
                     /locus_tag="ACHGNS_002239"
     CDS             complement(4481..5380)
                     /gene="era"
                     /locus_tag="ACHGNS_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFN6938162.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5395..5790)
                     /locus_tag="ACHGNS_002240"
     CDS             complement(5395..5790)
                     /locus_tag="ACHGNS_002240"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFN6938163.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5774..6250)
                     /gene="ybeY"
                     /locus_tag="ACHGNS_002241"
     CDS             complement(5774..6250)
                     /gene="ybeY"
                     /locus_tag="ACHGNS_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFN6938164.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6267..8459)
                     /locus_tag="ACHGNS_002242"
     CDS             complement(6267..8459)
                     /locus_tag="ACHGNS_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFN6938165.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8483..9454)
                     /locus_tag="ACHGNS_002243"
     CDS             complement(8483..9454)
                     /locus_tag="ACHGNS_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFN6938166.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10160..11539
                     /gene="brnQ"
                     /locus_tag="ACHGNS_002244"
     CDS             10160..11539
                     /gene="brnQ"
                     /locus_tag="ACHGNS_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFN6938167.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
     gene            complement(11693..12139)
                     /locus_tag="ACHGNS_002245"
     CDS             complement(11693..12139)
                     /locus_tag="ACHGNS_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFN6938168.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12166..12342)
                     /gene="rpsU"
                     /locus_tag="ACHGNS_002246"
     CDS             complement(12166..12342)
                     /gene="rpsU"
                     /locus_tag="ACHGNS_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFN6938169.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(12504..12932)
                     /locus_tag="ACHGNS_002247"
     CDS             complement(12504..12932)
                     /locus_tag="ACHGNS_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFN6938170.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGKIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            13024..13884
                     /locus_tag="ACHGNS_002248"
     CDS             13024..13884
                     /locus_tag="ACHGNS_002248"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFN6938171.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
BASE COUNT         3822 a         2881 c         2501 g         4696 t
ORIGIN      
        1 ttcataggtg tttttaaaag ttacacaaaa aataaaataa caaaaaaatt aacattgaat
       61 taataatagt tatttatatt tataacaaat aaaccagaga aatagcctat tttgtgcctg
      121 ttctctggtt tatttacata ttagaaatta ttttgtgatg atttgatcta aacttgctag
      181 tgacaaagcc atggcagcaa tttgacttaa ttgcgttaat cggttcttac gtactgctgg
      241 ttcttctgcc atgaccatcg tctgatcaaa ataattttcg atcagtgggc gaaggcttac
      301 aagctgatca taattttcgt tgatcgtttg gtcagcaaag cttgttttca ttgcctctac
      361 tgctttgtgc agttctttct ccgcttcgtt ttcgaacagt tcagggtcta cttctgattg
      421 tttttcaagc tccgcttttt tcgctaagtt cacgacacgt gtcaaagctt ccatcgatgg
      481 tttgaagtct tcatctgcaa gatgctgctt gaacaattgt gctgctgcaa atacttttgt
      541 cagatctttt tgttctgcat tcaaaacagc ttcgatgaca tcatgacgga tattcttcgt
      601 tgtcaataat tggcgcagac ggcctttgat aaagtcgatc acctgttgtt gtcccgctga
      661 caagccaatg ccaaaacgat ccgtatcttt actgataact tcactaatcg tttcttgcag
      721 aacagacaaa ggaaaggccc attttttcga ttcgatgatt cgaacgacac catatgcttg
      781 acggcgcaaa gcatatggat cgtttgagcc acttgggatc atcccaactg caaagaaaga
      841 aaagatacta tctaatttat cagcaatcgc taaaacagaa cctactgtag tctcaggtaa
      901 atctccttca ctggatatag gcatgtaatg ttcacggatc gctgttgcta cggctggttt
      961 ctcgccttta agcagagcat atttttcgcc catgatccct tgtagttcag ggaattctcc
     1021 taccatgttg gttactagat caaatttata gatttcagca gcgcgttgca gatccatcaa
     1081 ttcttcctca gatagtccta cttgttcgcc tatgatgcga gcgatgactt gtacccgttg
     1141 cattttctca tagatgctac cgattttttc atggaaagta acagttttta atttttcaac
     1201 gtattggtcg attgaagctt tcttgtcttc attataaaag aattccgcat cttctaaacg
     1261 agcagtcaat actttttgat tgcctcgtac cacattttcc aattttaccc gatcgccatt
     1321 acgagcagaa ataaaatgag gaagcaacat accttgatcg ttccgtactt cgaaataacg
     1381 ttgatgttct ttcatagaag tgactagtac ttcttcagga acagatagat atttttcttc
     1441 gaagtcgcca gaaaagacag ttggatattc gactaaattg ttgacttctt ctagtaaatc
     1501 ttcatctaga tcaatgatcc agtgattttt ttctgctaat tcgtttgctt gtgtgatgat
     1561 catttctttt cttttttgcg gtgaagcaat aacaaattgt tcattcaatt tttcttcgta
     1621 ttgttttgca tgcgtaaatg tcacatcttc gcctaagaaa cgatgacctc ttgaagctct
     1681 gtctgttttg acatccaaga tctcaaacgg gattatttcg tcatctaaca atgccacgat
     1741 ccagtgaata gggcgaatat attcaaaatc gtattctgcc cagtgcatcg ttactgggaa
     1801 agtgaggctt ttaatgacat ctgctaaacc tgtcaatact tcttttgctt ctttgccgtg
     1861 agtgtatttc gttacataga catattccac gccttttaat tctctaaaag tgatagcatc
     1921 tgtcgtcaga ccttgtccgc ggacaaatcc ttcagctgct ttcgtccagt ttccatctgc
     1981 gtccaaagca atttttttgg ctggtccttt gacttcttct tcgctgtctg cttgacgttc
     2041 tggaatgttt gttactttga ttgctaagcg gcgcggtgta gaaaacgttt cgatcgtatc
     2101 ataagtcaat tggttttcat ctaaaaattt tgctgttttt tcttctaatt gtttcatgct
     2161 tggtgtgact acatgagcag gtatttcttc cagcccgatt tcaagtaata agtcttttgc
     2221 catcttattc agcctccttc acgttttctg gatttgctgt gtttttattc aataaaggga
     2281 atcctaattt ttcgcgttct gccacaaaga ttttggctac agcacgagcc atgttgcgga
     2341 tacgggaaag ataacctgca cgctctgtta cggatactgc accgcgagca tctaataaat
     2401 tgaatgtatg gctacatttc aaaatataat catacgctgg atgaacaaga ttttcatcga
     2461 tacagcgttt agcttctttt tcaaaacgat caaaattgct tagtagcagg tcttggtcgc
     2521 ttacttcaaa agaatattta gaatgttcat attctggttg tttgaagatc tcaccatatt
     2581 tcacgccatc agcccattcc agatcataaa cactttcaac ttcttggata taagaagcaa
     2641 gacgttctag tccataagtg atctcagaag taactggttt acaaggtagt cctccgactt
     2701 gctggaagta tgtgaattgt gtgatctcca tcccatcaag ccatacttcc cagcccacac
     2761 cggcacaacc catcgatggg ttctcccagt tgtcttcaac aaagcgaata tcgtgttcta
     2821 atggatcaat tcctaaaaga cgcaagcttt ctaaataaag ctcttggata ttttcaggtg
     2881 aaggtttcat tacgacttga aattgatgat gctgatacaa acggtttggg ttttctccat
     2941 aacgcccgtc agctggtcgg cgggatggct ctacataagc agcattccat ggttccggtc
     3001 cgattgcccg taagaatgta tatgggctca tcgtacctgc tcctttttct gtatcataag
     3061 cttgcatcag catgcagccg tttgatgacc aaaatttttg taatgttaaa atcatatctt
     3121 ggacggtcaa ttttttactc atctgttttg cccctttcaa tttttaagcg ttgctgtcaa
     3181 taagaataca ggaaagaaaa cgacaaaaag tccctatgtc tgattacaga catagggacg
     3241 aataatttcg cggttccacc ctacttccga ataaagattc ggcagcttca tttatcgtac
     3301 tcgcaagtgc ctcttcaaaa atcgtttggg tccggcttcc actctcccga actcgctaaa
     3361 ccacgtgttt tttactgatc ttggtcaatg tacgtattca ttttgcagtt ttctggtttg
     3421 tataatagat cctctcagtg aacgatctat ttaatctacg gttaataata gacggaagtc
     3481 cctgtatttg tcaaatagtt ttgatttttt tgttgtatct ttcatttatt cttctttttc
     3541 aggtatctct ttttcaggca tatttttctc tggcgtcttt aatacgtgtg cccaagactt
     3601 catttgatca atgaactttt tgctttttag atgaataccg acatattcat catacaactg
     3661 gtcaatgagg tcacggattg cctgcttcgt ttcttctttc aagtggattc cactgatctt
     3721 atcataagaa atagccgaaa acattcgaac gaaataaaca gcgcgaggat ctgcatgata
     3781 acggtgttcg tccattggcc agtgtttctc gcatatcaca ccgccatatt tggaagaata
     3841 gtcaaatttt ccttttgttt ccccgcatac gacacagtgc gtccaaattg gcgcgattcc
     3901 gaagcgttgc agtatttgca cttcaaagat attcgtgatg atttctgcat catctccttg
     3961 gtccatccgc tgcagagctt gttgcgtaaa ttggaataaa tgcgggtcat attgatgatc
     4021 ttcgattgct gcatctacta gatttagcaa atacgtacca taaccgctca aaaagatatc
     4081 ttcttggatg ttccgaaacg catggacgtc tttgacactg cttaaaaacg acaaaccttc
     4141 acttcgaaaa ttaccgatat agactgcttc ggtaaagggg agcaaagccg gagctaatgg
     4201 ctgattttgc cgatggattc ctttgatata aaacatcatt ttgccagaag attccgtaaa
     4261 gattttgact aatttatctt tttctttgta attacggcta gataaaatca agcctttcga
     4321 ttctgcaagc tggctcatat tctccctcct aaaaaataaa tctggaaaag cagattctgc
     4381 cgatccagat tatttatgtt tcgatcttcc gctgtttttc tcaacgtacg gatttttatc
     4441 cagtttattt ctatacctaa cctataagaa gtccagaata tcagtactcg tcttttcggt
     4501 agccgaagtc ttgcagatag acttttttgt ctcgccagtc tttttggact ttgacccaaa
     4561 gttctagaaa tactttgtct cctaaaagat tttcgatatc ctttcttgct ttcgttccta
     4621 cttgcttcag catttttccg cctttgccga taatgattcc tttttggcta tctctttcga
     4681 cgatgatcgt ggcctggata tggattttat cattctcgtt tcgcttcatc gaatcaacaa
     4741 cgactgcgac agaatgaggc acttcgtctc gtgttaaaaa caagactttc tcacggatca
     4801 attcagaaac gatgaagtat tcaggatgat cagtcacttg atcatctgga aaatattgtg
     4861 ggccctctgg catttcactg actaatacat ccattaagcg ttcgacattg tttccttctg
     4921 tagcagagat cggaatcact tgtgcaaaag gcatctgcgt cgaataatct tcgatgattc
     4981 ccagcaactc gtctggatgg atcttatcga ttttattgat aatcagataa acaggagaat
     5041 gcacgttttt tagacgttca ataataaaat cgtctccccg gccacgtttt tgatcagcac
     5101 tgatcataaa taagactgca tccacttctt tcaacgcact gtaagctgct tctaccataa
     5161 aatctcctag acgatgcttt ggtttatgga ttcctggtgt atcgataaaa atcagctgag
     5221 cttctggtgt agtatatact ccttggattt tatttctagt tgtctgtgcc ttgtcactca
     5281 taatggcaat tttttgaccc acgattcggt tcaataatgt ggattttcct acattcgggc
     5341 ggccgacgat tgcgacaaac cctgatttat gttctggcat atacgttctc ctttttaatt
     5401 aaacaacaaa tgatatattt tagggacaaa taaaatcaac ccaatgatga cagcaaaaca
     5461 agcggtcact aaaactgctc cagctgccat atctttgatt tttttcccaa tcggatgaaa
     5521 atgaaaatcc gtaaacatgt ccactacatt ttcaaaaaca gtattgatga tctctactat
     5581 ccaaacgaga aaaacagcta aaagcagcca taaccactct atccgatcaa gctggaaaat
     5641 cacgcccata aataaagcaa ggataccgaa cgcaacatgt ttcctcatat tgcgttcttc
     5701 ctcaaaaacg gttttgactc cttggacagc aaattccaca gacatcagga aatgtttgtt
     5761 tttctctacg tttttatctt ttgagtccat aggcatccaa gatctctttc tgtaaaccaa
     5821 acatttcttt ctcatcttct ggtgtcatat ggtcataccc gttgatatgc aagaatccat
     5881 gtaccgctaa aaagccaagt tcccgatcat atgaatggcc gtaagcttct gcttgttcag
     5941 cagcacgttc aatagagatc atgatgtctc ctagtacacg aggtaatgga ttctctccat
     6001 cttcaaaaat cactgggatt tcgtcttcgc cttcttcttc cagagcaaaa ctgatgacgt
     6061 ctgtcggtgc atcttttcca cggtaatcac gattgattac ttggattgcc gcattgtcca
     6121 tgaaagtaac agacatttct gtttcctctg gcagctttaa ataagatgct gcaaattgca
     6181 gcaagtcatc gatttctttc attttctctt ctgagacttg gtttgtttca tccataaagg
     6241 taatatccat tatacatcgc ttctttctat tcttcttgtt cttcctttat cttctcaact
     6301 tctgttttca ttttaggata tttgatccga gaatggaagg tgctacttaa accgctgatt
     6361 aatgattttt ccacttttcg gatttctttc aaggtcagtc cactatcgtc taattgacca
     6421 tcggaaatac gctcttcgat aagattatgg acaaaacttt cgattttttc gctcgtcgga
     6481 tgatccattg cccgtactgc agcctcacaa ctgtcagcaa tattgacgat ccctgcttca
     6541 cgactttgcg gttttggtcc aggataacgg aattgctctt ctgttacttc cggattgcgt
     6601 tctttcgctt tgatgtagaa ataacgcatt agcgtggttc catgatgctg tttgcaaata
     6661 tcgatcacca tttgcggcat attataatct tccaatattt tggctccatc tgtcacatgt
     6721 ccaaagataa tatgtttact gtcttctggc aataagaaat tgtgcgggtt ttccgctcct
     6781 gaaggcaagt tttcaacgaa aaagctagca tgtttgatct tgccgatatc atgataatag
     6841 caagctactc gagttagcaa agaacgtccg ccgatttctg ctacagcgtt agcacttaga
     6901 ttagctacca tcatactatg atgataagta cctggcgctt cttccagcaa ctgtttcaat
     6961 aaaggatgat ttggattgct gagttcattt aatacaatta cactgtcatc attgaccatc
     7021 aattcaatat atgggtgtag accgatcgtc agcaagaatg agaagagtgc accagtgaac
     7081 ccgcagacga gcattaacca agtcgttgca tcaccaaagc tcattccttg atagacgatc
     7141 aaaatgacat ccatcatcac aggaaaaata atgacccaca tggcagcaga taatccttgt
     7201 tctgagatcc gtttgcgtct gacaatcgtt gccatgagcc cagagaaaac ataagacatc
     7261 aagataatcg ttagaaagtt cgtgccaatc gaatcataaa aaatgaaaag tgcggatact
     7321 gcttggaaaa taccagccat gatccctgct cttcgattca agaaaaagct taaaacaagt
     7381 ggtgcaaaag cagcgggata aaacagcggg atataagtag cttgttccgt ttggaataat
     7441 tggaaaaatt tcatcagcac tatgctgact gacatagctg tcacatagaa aagcacgtat
     7501 tttgttcttt ctcctgcatt ttggaattga agtgaattat aaatcagtac gccgatttga
     7561 agcaaaacag ctaaaaccat ggcaattagc ggaaaaatcg aagttgtttg attagtcagt
     7621 cctagtaatt ccaatttttt catagctgaa gcatcgattt gattaccttc acgaacaatg
     7681 atttctcctt ggaagatcat cactggttgt acagcttctc gtgcggattg tttcaattct
     7741 tctgtctttt tctcattcaa tacgtcattc actacgatac cttgatccac taagtagcga
     7801 atcatttgtt gttgtgctgg tgtgacattc aaatattgga tttgctcttc tgcttcttgt
     7861 ttaaaagctt ccaattcact ttcacgaact tgtttgctca tctgttcgtc aatgagcttc
     7921 agactctcat ccctcacagt atgaatctca gtttcagtca tttcaaatat cgtcgaataa
     7981 aaattgttcg gtagtttttg ataaaacaca acattttctg cattcacatt ttcaaaattt
     8041 ttcttcagcg cggcgatccg ctcttctgta gtaggctttg ggatattttc atcttccttt
     8101 gccttatcaa tcttttcgtc ataagatttg ttgatggcat cattcgtctt atcgatcaat
     8161 tcaaatagtt gcttgatccg attgttctgg tcatctgcaa gatctttttg gtaagtgtat
     8221 tctggagtga ctgcttctgc tgccagtttt cttttttgtt cggtttcttc cgtgttctcg
     8281 atcgttttgt tcgcacggat actttcttct gctacttggc cttctctgaa atcattggcc
     8341 ttttgtttga cactactaaa tgtaacgccg cagataatga taaaaaacaa gaacagcagg
     8401 aaaggcaatg ctgcctttcc aagtttttct tgtagtttct gcagtgtttt attcaacatg
     8461 tgcttatcct cctaagcaaa gcttattctt gatggagatc tgccttttca taagctttga
     8521 tgatttcggc aactacaggg tgccgaacaa cgtcacttgc ttcaaaatta acaaaatcga
     8581 ttttcttgat ttgctttaat gttcgttctg catgaacaag accacttgtc gtccctcgag
     8641 gcaagtctat ctgacttgta tccccattga caatcatttt tgattgataa ccgagacggg
     8701 tcaagaacat tttcatctga gcgactgtcg tgttttgtgc ttcgtccaag ataacgaatg
     8761 catcatccaa tgttcgcccc cgcatgtaag ctaaaggtgc gatttcgatt acgcctcgtt
     8821 ccatcaatcg gttcgtatga tccaatccaa aaatttggta taaagcatca taaacaggcc
     8881 gcagataagg atccactttt tcttttagat ccccgggaag aaagcctaga ttttcacctg
     8941 cttccacagc aggccgcgtc aaaatgattt tttgcacttc accttttttc aaggcagcga
     9001 ttgctaatac tactgcaaga aaagtttttc ctgtaccggc tggtcctacg ccaaatacga
     9061 tatcatgttt ggctaccgat tcgacatatt tcttttggcc gctgtttttt acccgtatag
     9121 gttttccatt acggtctttg acgatctctt cctcatacat ctcaacaaaa taatctaatg
     9181 tacctttttg ccccattttc aatgccgtca caacatcggg cgtacttacg tggattcctc
     9241 gtttgatcaa ttcctgtaat gtccgaatga ctgaggcagc caaggaaatc tgggattgct
     9301 caccaatgat ctgaatgatt tctccacgtg tatggatggt tgtttccgtt gtttcctcaa
     9361 tcagttttat atgcttgtcg tgtgcaccta aaagcatgct gacatcatca gcagccgtca
     9421 atctaatctc taaagaacta gactcttccg tcaaaaatag acatcccctt tttttcgatt
     9481 cattctttcc tattttacca tactcagaag aaactatata gcaaataaga aaaggttcta
     9541 aaagagttgc ttaaccataa gtaccaagaa acgttaaaaa actagccgct tccattctct
     9601 aaaggtctcg tttgaataaa aaaagctgat catgtaagct atgcataaga tagaggacaa
     9661 gtattccgtc taacttagca agtattctct gggtattttt cttgtttcaa aaaaagaaaa
     9721 caaaatattt gaaatattct tgcatttcaa aaaaaacagg tgtattataa cgtcaacaaa
     9781 taaatataca gcaaagcgtc gaggaaaaca gtaggctatt cagtgattta ttcccagtga
     9841 gtcatgtaag tgagaaatga caataaatcg aatagacgaa catcatttcc gagtcaatgt
     9901 cgaaaacgta gatcattgtg gctgcctcca ttatcaggct agcggataac tttatcgaaa
     9961 agcgtccgtg aatgaagaag cttttttaag cgaaaaaagg tggtaccgtt ctagaatcta
    10021 gagccctttt cccaagtgga aaagggcttt ttttatattt taaggtggtg gttttcatgg
    10081 aatctgatat tagtggtatt tctagtttct gcaacgttta ttttgttact caaaatgata
    10141 attattggag gaaaaattta tggaagaaac aaaaacttat tcatggaagc aattattgat
    10201 ggtcagttca cttatcttcg ggatgttctt cgggtcaggt aatctgatct ttcctgtaca
    10261 cttaggtcaa atggctggat caaactggat cagcgctggt gtaggatttg ccatctcagg
    10321 cgctcttttc ccattattag ctatcttagc tgtagtagtg acaaaaagtg atggtctgta
    10381 tgacttagct aaacctgtcg gaaaatggta tgctgccttg ttcttgatcc ttgttcattt
    10441 aacgatcgga ccattctttg gtacaccgcg gacagcagca actgcttttg aaatggctgc
    10501 taaacccttt ttgcctgaaa aatatgattc attaggtatg tttttattct ctgctctttt
    10561 ctttttactt gcttatttcc ttactgtaca tccaacacgt ttggtcaaat atgtcggaaa
    10621 gtatttaaac tccttgtttc ttgcattatt agctatcatt tttgtcgttt ctttattcca
    10681 acccatgggg aatctaaacc aatctgctgc agattcttat cagacgaacg ctttgacaaa
    10741 tggcgtttta gaaggataca acacggttga tgcagtagct ttacttgctt taagtgtcac
    10801 ttttgtccat gcagttcgtg atcttggttt taaagataaa aaagtcacag aattaacagc
    10861 aaaagctggt agcctgagcg ttgcgttaga agtcatgatt tattttggtc tggtccttct
    10921 cggtgcgatg agtttgaata agatgtcttt atcagaaaat ggcggaactg cactttctca
    10981 gatcactaat ttttatttag gtaacttcgg tgctagcttc ttaggggtaa tggtcacatt
    11041 aggtgtcttc actactgcta tgggactcgt tgtttctttt gcacaagatt tccataaact
    11101 gttccctaaa gtcagttata tgacatggct gcggttaacg acttttgtct cttttgtcgt
    11161 tgcgaatgcc ggattggata atatcatcca atggtcttta ccagtattga tgcttttgta
    11221 tccattgtca cttgcgttaa tcttactttc cttaacagca aaattcttcc aaaagagtcc
    11281 atttgtctat caagtaacga tgttcttttc tgccgttcca gccgtattgg atatgttggc
    11341 aaattcgcct gcattagtga gtcagcaaac tgtcgtagca agcttactag gattttatca
    11401 tcattatgtt ccttttgctg cattaggtct tggctgggtc gtcccaacgt tgcttggtta
    11461 cgctggcagc ttgctctttt attatgtata tcgcgtatca ggatataaac aagaagcaag
    11521 tgagttacca gaagaataac caaatcaaac acaatttaac ataagagata ttaaacagga
    11581 aaaaaacagc tagaagaaag aataagattc tttcttctag ctgtttttta gttctttttt
    11641 tacgaacaca aaaaactgcg gataatcact gtccgcagtt taaatgataa aattaagatt
    11701 gtaatagttc tttcacaatc gcattcactt ggtttccatc tgcttttcct ttgactttag
    11761 gcataattgc acccataact ttgccaaatt ctttcattga tgaagcacca gtttgagtga
    11821 ttgcttcttg aacaagttgt cgaatttctt cgtcggaaag ttgttccggc atatattttt
    11881 cgactattac aatttcactt tttacttttt cagctaaatc gtcacgtcct gcttcttcaa
    11941 actcatgcaa agaatcacgt cgctgtttca tttcacgaga caacaccgtt agttcttctt
    12001 cttctgtcaa gtcgtgacct tctttaatct gctcgttttg tatagatgac ttgatcattc
    12061 ggatgacttg taaagattcc ttatctttcg ctttcatcgc tgctttcatg tcatcgttta
    12121 atctacttag tagtgacaca ctaccaactc caattcatca cagaactaga attttttgcg
    12181 ttttctagct gcttcagatt ttttcttgcg tcttacactt ggtttttcgt agaattcacg
    12241 tttacgagat tcttgtaaag tacccgcttt tgacacggaa cgtttgaagc gacgaagagc
    12301 atcgtcaagt gattcgtttt tgcgaacgac tgtttttgac atgttaattc cctccctccg
    12361 agcttaaaaa gtaaccgtaa ttgttattga aatatatctc aaaaaacaag actgtcctgt
    12421 aaatattata tctaatgctg ttttgaccgt caaggaaatc ttgctttttt ttaaaattat
    12481 caaatgttat tttatttaaa tccttattga caattttcac atcttccgaa aatctcgaat
    12541 ctatgccctt cgatcgtgca tccacctagc tgttcttcga aataattcat cggacacata
    12601 tggatttctt tcgttgttcc gcacactgta cagataaaat gatggtgatg gccgattttc
    12661 ccctgacagc aatgaaaacg aaacttcatt tctccattta aatcggtttc ttccagtaaa
    12721 tcgatctctg caaaatcatg gagattccgg taaatcgtat cataacttac ccctttgaat
    12781 tcctgattca tatagtcaaa aacttcttta gcagatacgt agcggttacg cttcactaaa
    12841 taagttaaca atgcttctct ttttttagta tatttaaaac cgttattttt caatgtttct
    12901 aatgattgtt cgatcaatgt ctgttttttc atgacttatt cctccaaatc aaaatgatta
    12961 cgaagtacgc attctagagt atggtataat aaacataaaa aaaatgcaag tgaggtttta
    13021 tccatgtctt ccgattctac agaaatcgtt actttttttg tcatctccga ctctgctgga
    13081 gaaactgcga caaaactagc gcaagctact atggctcagt atcctaccgt tgagttcaac
    13141 ctattccgca gaacgttcgt gacagatgaa aaaacattaa ggcaggcatt gcaagatgca
    13201 ttagaagaaa aagcacttgt cctgcataca ttaatcaatc aagaactgat cgagataaca
    13261 cgttcttttt gtgaagaaca tcaattattt agttttgatg tgatgacgcc accgatatca
    13321 gaaattgaac gtttaactgg tatcaagcca attcggcaac ctggtgccct ccatttattg
    13381 aatgagaact atttcaaacg gatcaaagca atggaatttg ctgtaaaata cgatgatggg
    13441 aaagatcctc gtggtttttt agaagcagat gtcgttttgc taggcgtatc ccgaacttcc
    13501 aaaacgcctt tgagcttgtt tttagctaac aaaaatttaa aagttgccaa tttacctttg
    13561 atcccacaag cacatattcc caaacagctt tgggagatcg atcctaaaaa aatcgtggga
    13621 ctaacgaata acccagatat tttaaataat atccgtaaag aacggatgcg ctcttacgga
    13681 ttaaaccctg atactgctta ttccgatatc gaaaaaatta gagctgaact tgattttgcg
    13741 aatgatcttt atgaaaaact aggatgtgta gtgatcaatg tggcatcttt atctattgaa
    13801 gaaacggctt cgcttatttt gaatgcttta gacctagaag atcatagtta ttatggaact
    13861 gaaacaacag aaaaaagcga ttaatcattt ttgtcttttt
//
LOCUS       DAZBTF010000071        13828 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000071 DAZBTF010000000
VERSION     DAZBTF010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.071"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            103..2268
                     /gene="acm"
                     /locus_tag="ACHGNS_002249"
     CDS             103..2268
                     /gene="acm"
                     /locus_tag="ACHGNS_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFN6938172.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2540..3025)
                     /locus_tag="ACHGNS_002250"
     CDS             complement(2540..3025)
                     /locus_tag="ACHGNS_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFN6938173.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3115..3460
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3505..4146
                     /locus_tag="ACHGNS_002251"
     CDS             3505..4146
                     /locus_tag="ACHGNS_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFN6938174.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4165..4800
                     /locus_tag="ACHGNS_002252"
     CDS             4165..4800
                     /locus_tag="ACHGNS_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFN6938175.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4818..5633
                     /locus_tag="ACHGNS_002253"
     CDS             4818..5633
                     /locus_tag="ACHGNS_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN6938176.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5952..7388
                     /locus_tag="ACHGNS_002254"
     CDS             5952..7388
                     /locus_tag="ACHGNS_002254"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN6938177.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKSSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7577..10009
                     /locus_tag="ACHGNS_002255"
     CDS             7577..10009
                     /locus_tag="ACHGNS_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFN6938178.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQLSFVPI
                     DSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQDYELPT
                     VDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEVQPAVG
                     VKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIEAQPNH
                     PDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRAKPHEV
                     KLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAATGVRNI
                     SGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMARAAGI
                     HMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGDMLFLP
                     MGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQPQDELF
                     EEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQE
                     ETSETLPDDNDM"
     gene            10239..11306
                     /gene="rlmN"
                     /locus_tag="ACHGNS_002256"
     CDS             10239..11306
                     /gene="rlmN"
                     /locus_tag="ACHGNS_002256"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFN6938179.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            11296..11502
                     /locus_tag="ACHGNS_002257"
     CDS             11296..11502
                     /locus_tag="ACHGNS_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938180.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(11499..12602)
                     /locus_tag="ACHGNS_002258"
     CDS             complement(11499..12602)
                     /locus_tag="ACHGNS_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFN6938181.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13154..13702
                     /gene="aac(6')"
                     /locus_tag="ACHGNS_002259"
     CDS             13154..13702
                     /gene="aac(6')"
                     /locus_tag="ACHGNS_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFN6938182.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         4735 a         2296 c         2934 g         3863 t
ORIGIN      
        1 agttaaaaga ttggaaatga gtgggggagg aattgaagaa aggatttttg agagatgata
       61 tagtaggttt tatttttagt taaaataggg gagatagaaa aaatgaaaaa gtgtagaaat
      121 attattttat caatgttatt tattattact aatataacat cactgattcc tgttcatgtc
      181 tatgcggatg caggcagaga tatcagcagt aatgtcactt cgttgaccgt ggacccaacg
      241 aatattacag atggaggaaa cataaaagta aaattctcgt ttgacgaaaa aaaacaaaat
      301 atccaaccag gtgattatct ttggatcaat tggccaagtg aaggaaatat tcgcggtgaa
      361 ggattccaaa aagaaatccc attaatgatt gaaaataaaa atgttggaac tttaacggtc
      421 agaaaagatt ctgcgcaggt tgtgtttaat gaaaatatta aaaacctaga ctcagtagaa
      481 ggttgggggc aatttgagat acaggccaga aacgtaaccg ataccggtga agaaaatacg
      541 ggaccattca ccgtgacgag cggtgataaa acagctactg taaatgtgac aaagccagct
      601 tctggttctt cttctagcgt tttttattat aaaacaggag acatgctacc tgaagatact
      661 aagcacattc gatggttttt gaatattaac aataacggca cttatgttga acaaccagtt
      721 aaaatcagtg atgaaatcca aagtggacaa agattagatc caagtacgtt tgaaattaat
      781 caaatacact taggtgaaca gaaggtttat agaggagaag agggaatcca acaattttta
      841 caagatttcc ccagcgctac atttaatttt tcagtaactg ataattacat tgaaataact
      901 ataccaaaaa actttgtcaa tcttaggaaa ataatggtga gttataagac gatcatagaa
      961 aacccagaac aaataaattt tgagaatcat tcggaagctt ggtttaaaga atttaataaa
     1021 cctgctgttg atggagaaag tttcaatcat acagttaaaa atatttcagc ttctggtgga
     1081 gtcaacggta cagttagagg cgaattgaag attttcaaat atatcaatga cacagagatt
     1141 ggtatcccaa atgtgacttt cgaattaaga cgagcagatg aacagcctat tcaaggacag
     1201 tctagtatcc tgttgacttc gaatgaacag ggagaagcta gtatcaaagg cctgcaagta
     1261 ggagactatg ttgtaaaaga aaaagaagca cccaattgga ttgattttga tccactaagt
     1321 tcaaatgaac tcaaattctc cataaacgaa aatgatacag agggagtcag tttacctatt
     1381 tataacaaga aaaaggttac gaacatcaca gcaactaaaa tttggaacgg tggaaccact
     1441 ccaagaccga gtatttattt taaattattc agagcaagta caaacaattg ggaaccagtt
     1501 cctgatgcag aaacaaaacg attagacaat ggaataactt cagtgacttg ggaagatatc
     1561 caacagtatg atgatagtgg aaacgaatat acgtttaaag tgcaagaagt agatcaaaac
     1621 ggaaatgatt atgtcccttc aggttatcaa aaaattgaaa acgggctagt cgttacaaat
     1681 gagaataaag aagtcatttc agtctcagga caaaaaacat gggaagacaa tgagaaccaa
     1741 gacggcaaac gaccgacgac gatcacagta aatctattag ccgacggtca accgatccaa
     1801 cacaaagaag taagtgaaaa agatgattgg cgctatcgtt tcacaaatct gccaaaatac
     1861 agagacgggc aagaaatcat ctatactgta acggaagaca acgtgcccga atacagtacg
     1921 acgattgacg ggtataacat caaaaacagt tatacaccgg aaaaaacaaa tatatctata
     1981 ccaggtagcc atataacacc gtggaaaaat tttccagaga agacagagag agagaaaatt
     2041 agaaatctat tctctcaaaa attgcaaata tttgctcata caggagaatc cagcgaacaa
     2101 agaagcaatt atattagtaa gagagctgta cctaaacaga taacaaataa atcaaaaagc
     2161 attcttccta aaacaagtag taaaaaatct agttttgctg taatcattgg tttattgata
     2221 gtaactatct gtgctgggat attcttacaa aaatataaaa aagtttagtt agaatataat
     2281 attttttgtt aaatatgact gaaatcaaaa gaataaaatt ccgatttata atcgaaaaaa
     2341 gaagcatttt tatatatgct tcttttttcg tagaatcaat aaatatggat ttggctaact
     2401 atggaagaat actcgtatta gttttcagcg ttcatactag ttgttacatt gtttgattgg
     2461 ccaaaagaat ttctgatata ttttgtgcta aataaagaaa aagacgcttg aattgggaaa
     2521 taagaaaact actctatcat tacaaatttt cataaactgg cagatgttct aagtaaacaa
     2581 gtatcttttc aaagtcggaa aaatccataa tcaacgtagt ggtattgata tttggatgaa
     2641 agcctacggt atcttgacga tcaatagcag catcgatcac gacttggatt tttttctctt
     2701 tgtcgtgcat caatgctaat ggagtcactg tacctgctgg aacgcctagg aggtcaggaa
     2761 gctgttcttc agaaacaaaa gataagcgct tttcttttaa ttgttcagct agttgtttta
     2821 aatcggcgtg cttttcatca ggcaagatga caagatagat ttgttttcct tttcttgatt
     2881 tcaataatag attttttact tgcggaccag gtagggaaaa agggacattt ttgactgacg
     2941 taatcgcagg atgatcgact ttttgaaaag agatacccag tttttttagt agttcataag
     3001 cttcttgttc ggttgcacga ctcatcgcgc atcaccctcc tttttttatt agtgtatcat
     3061 aaaaaagagt tgacaaatga aaaataatca tgtaaattac acgtaaagtt aaaacaaatg
     3121 acatcgaaaa gaagagtagc cgttaggtgt ttttagagag tctctggttg gtggaaagag
     3181 gcagcagtta accgtgaaac acatcttgga gtcgattttc tgaagcgtat agtaggaaaa
     3241 tccggggagc ccgttacagc tgcggtcgtt gctagatgac cagataaggc tggcttttcc
     3301 gtgaggagtc ggtaaaaaaa ggtggaacca cgtgattaac gtcctttgat cttcggatca
     3361 aaggactttt tgttttcatc tgaacggcaa cgactgtgtg aggttttttc tgcaaggaaa
     3421 aaataaacag aggtggaacc acgtataata cgtcctcttg gcatttgcca agaggatttt
     3481 tttattttaa aggaggaaaa gaagatggag tatttgattg aagttatgcc gcaattatta
     3541 gtaggagcac tcacaacatt gaaattattt ttatttacct tgattggctc attaccatta
     3601 gggattgttt tagcattagg attgtctttg cattttaagc cgctacgcta cttgcttcaa
     3661 acatatgtat gggttatgcg gggaacaccg ctgttgttgc aactgatttt tattttttat
     3721 gggttgccga cgattgggat cgtttttgat cgtttcgaag cagcactcat tgcctttatc
     3781 ttaaactatg cagcttattt tgcagagatc tttcgtggag ggatacaatc gattcctcaa
     3841 ggtcaatatg aagcagcgca agttttgcaa ttaagtccct ttcaaacggt gacgaaaatc
     3901 gtcatcccac aagtgacaaa gattgtttta ccttctgtag ggaatgaagt tatcaacttg
     3961 gtcaaagatt cttccttgat ctatatatta ggtttaggtg acgtaatgcg tgctgggaaa
     4021 atcgcgatgg aacgcgacgc gactttactg cctttgctag gcgtggcaat catctatctt
     4081 acattgactg gtattctaac aatcgtaatg aaacaagttg aaggaaaatt aaattattac
     4141 cgctaactga taggaggaag aaatatgatt gaattgaaga aagtgaacaa agcatttggt
     4201 gaaaaaaaaa taatcaaaga tttgaatcta atcattccag atgggaaaac attagctatc
     4261 gtaggtcctt ccggtggcgg taaaaccacg cttttacgta tcttggctgg cttagaaagt
     4321 tctgacagcg gagaatttta tttagacggt gtaccgttca acccattcga gcttaaaatg
     4381 aaagaacaag ttgtaggagt cgtattccaa gacttccagc tatttccaca tctgaatatt
     4441 tttgaaaaca ttaccttagc accaaaaatg gtattgaaac agacaaaaga agaatacacc
     4501 aaaaaagcag aagaattagc ggcttcatta gggattagtg atttattgaa tatctatcct
     4561 tatcagcttt ctggcggaca aaaacagcgg gtagcgattg ccagagcatt ggctatgaat
     4621 ccacgggtat tggcttatga tgaaccaaca agtgctttag atcctgaatt acgccagcaa
     4681 gtcgagcgtt tgatcctatc attgaaagaa gatggggtaa cccaaattat tgtcacccat
     4741 gatttagtat ttgcagaaaa tgtcggtgat caattactaa aagtttcccc aattcattaa
     4801 gaaaggagaa gatagagatg aaaaaaacca gttttctatt cgctttgatc gcaggtcttc
     4861 ttctttttgc aggctgttcg aacaaaaaaa cttcagcaga taattgggcg actatcaacg
     4921 aagagaaaaa aatcgttata ggtttagacg atacatttgt tcctatggga tttagagata
     4981 aagacgggaa gctcacagga tttgatattg atttagccaa agcagtcttt gaacaatatg
     5041 ggattacggt agactttcag cccattgact ggtcaatgaa ggaatttgaa ctgaacaatg
     5101 gaacgatcga tttgatatgg aacggctatt ccaaaacgcc ggcaagagaa aagaaagtgc
     5161 aatttacaaa accttatatg gaaaacgatc aagtgctgat cacacctaaa gaatcaggaa
     5221 tcacaaactt tggacaaatg aataataaga gattaggtgc gcaaaatggg tcatctggtt
     5281 atgatgtatt tacgaaacaa ccagaagtat taaaagagat agtcaaaaac caagatgctg
     5341 ttttgtatga tagttttaat gaagcattga tcgatctgaa aagcagacgg gtcgatggtc
     5401 tgctgatgga taaagtatac gcagactatt atttgaaaca aagaaacgaa ctagaagact
     5461 tcaacataac aaaaggttct tatgagagtg aggattttgc agttggtgca cgcaagtcag
     5521 atagtacatt agtgaaaaaa atcaatgaag ccctagatca actagagaaa acaggtgaat
     5581 tccaaaaaat atccgataaa tggtttggcg aagatgttac gccgagaaat taacaaatag
     5641 gggactgtga caaaagacgt gtcacagtcc ctttttccga ataaacagtg ttctcctgcc
     5701 aactccttgt gactcggagc tgaatggcag gctcacaacc tcttggtaaa cggtgttcaa
     5761 aagccagttt ttcggtatta agccaaattt atgcaaaaat gtagaaagcc attttcacaa
     5821 aatttttctt actactcgaa actaagcgac tttttcacaa cctcctttac ataattttat
     5881 tttgttgtgg aaggggtttc gtgttattct aaaagaggaa cgattgaagg ttataggaaa
     5941 ggaagagatg aatggaacac aaagaactta cctcatttcc aaaagatttt ctgtggggct
     6001 ctgcttctgc agcgtatcaa gtagaaggag cttggcaaga agatgggaaa ggagaatcag
     6061 tctgggatcg atttgtacgg atacctggaa agacatttaa aggaacgaac ggtgatttgg
     6121 cagtagacca ctatcatcgt tataaagaag atattgcttt aatgaaagaa caaggtctga
     6181 aagcttaccg cttctctgta gcttggacac ggatttttcc taatggacga ggagaagtca
     6241 atcaagctgg gctcgctttt tatgagagat tgatcgacga attgattgaa aacgatattg
     6301 aacctatgtt gactttatat cattgggatc ttccacaagc attgcaagat gaatataatg
     6361 gttgggaatc acgtcagatc attgaagatt tcactcatta tgcagaaaca ttatttgagg
     6421 cattccgcgg gaaagtccat tactggatta gtttgaatga acaaaatatc ttcacttcat
     6481 taggttattt gttagcagca catccgccag gtgtgacaga cccgaaacga atgtatgaag
     6541 ttaaccatat tgctaattta gcaaatgcat ctgtcatcaa taaatttcac gagttagaaa
     6601 tgcctggaaa aatcggacca agctttgcgt atacgccaaa ttatccaatt gacagtaatc
     6661 ctgaaaatgt tctggctgct gaaaatgcag aagacctgat ggccaattat tggatggatg
     6721 tctatatgtg gggaaaatat ccgattgctg ccatgaggtt cttagaagaa aaaggatggg
     6781 caccaacgat tgaagctggc gatgcagaat tgctagaatc tgctaaacca gattttcttg
     6841 ggatcaatta ctatcaaacg gcgacgaatg cattcaatcc attaaacgga gttggtgctg
     6901 gaaaaatgaa tacgacagga aaaaaaagtt catcagaaga aactggagtt cctggaatgt
     6961 ataaaaaagt tgagaatcca tttgtcgaac gaacaaattg ggactgggag attgacccag
     7021 aaggattaag gatcggactg cgtcgtatca caagccgtta ccgcatgccg gtactgatta
     7081 cggaaaatgg cttaggagag tatgacaagt tgacagaaga taaacaaatc catgacgact
     7141 atcgaatcaa ttacttacaa agccacgtca aagccatcaa agaagcaatt tcggatggag
     7201 cagaagtcct aggctattgc acctggtctt atacagattt gcttagctgg ctgaacggtt
     7261 atcaaaagcg atatggattt gtatatgttg atcaagacga aacacaagaa ggatctttgg
     7321 aacgatataa gaaagacagt tattactggt accaaaaact gattgaagaa aatggagaga
     7381 acctttagtt cacatactta aaacaagaag gtacggctgg atatggccgt gccttttctt
     7441 gataaaatca tttagaaatt aagaaaaact gtaaaataag ctgaaaaatg aaagaaagcg
     7501 tcagttcttt caaacaaaac tttagttccc ttactttttt ttgtataatg aaacttgatg
     7561 aaatgaggga taataaatgg cacaaaagaa acggcctact aaaaaaagga aaaagacgaa
     7621 aaaaacgaaa aagcaaaaac agcagcagga acaaatgctg acgattggtg ttgggtttgc
     7681 cttgatttta tttgctgtct tcggcttttt aaaattaggt tttttaggaa tattgattgc
     7741 aaacggcttt cggatcattg caggaaatac ttaccaaatc ctctgtcttt tattagccgt
     7801 attaggattt tggattgtca ttaagaatac agagttttca attggtaaaa accgacgctg
     7861 gtttggcggc atactctttt atttcggtat cttattattg ttacatgccc atttatttgg
     7921 caaactccat actggggaac caaatatcat ggggacgaca tgggatttgt tagccagtga
     7981 tataaaacaa agtcaagtag acaataacgt tggtggtggg atgattggag caatccttta
     8041 tcattttact tacttcttga ttgcacagcc tggaagttat ttagcagcag tgctattact
     8101 ggcaggagga gcattcctgt tcagtaatct ggaaggctat cagctgttaa atggtatcca
     8161 atcaattgga gaacgagtac aggaattatt ggaaggtgat cctgccaagc aagctcgaaa
     8221 gcaagcggca aaggaagaga gaatgaaaca gcgcgcagaa gcgaaagaag caaggcgctt
     8281 agctgcaaaa gaagctgcag aatatgagaa gaaaaaagcg gtgagccagc aaagggaaaa
     8341 tactgctgat gaatggcagg aaccagaaaa tacagagccg gagcagctaa gttttgttcc
     8401 aatcgacagt tttcaagaaa atatccatcc tgcgaatcta gagaaaccag taccagatac
     8461 accaaaacag accaatacag cagaaggttt ttcagatgag ttgccagagg atgacggtac
     8521 ctcattagaa tttgagattg aagcagaaca ggaaaatcaa gactatgaat taccgacagt
     8581 tgatttgctt gattctatac caactgtgga tcaaagtgat gagtataaaa aaattgaaaa
     8641 aaatatcggc gtattagaac aaactttcca aagtttcggt gtcgatgcca aggtcgtcaa
     8701 agccagctta ggaccatccg tcacaaaatt cgaagtccag ccggctgtag gggtaaaagt
     8761 aagtaaaatt gtgaacttaa cagatgatat cgcgcttgct ttagcggcga aagatgtccg
     8821 gatggaagca ccgattccag gtaaatcact gattggtatt gaagtaccaa atggaaagat
     8881 cagtatggtt tcctttagag aaatcatcga agcacaacca aaccacccag ataaattact
     8941 agaagtacct ttaggacgtg atgtttctgg acgggtacaa acggctgatc tttcgaagat
     9001 gcctcactta ctcgtggctg gttcgactgg tagtgggaag tcagtagcaa ttaatgggat
     9061 tatcacaagt atattaatgc gtgctaagcc ccatgaagta aaactgatga tgatcgatcc
     9121 gaaaatggtt gaattgaata tgtacaacgg catacctcat ctgttgacgc cggtcgtgac
     9181 gaaccctaga aaagcagcgc aagccttaca aaaagtagta caagaaatgg aagaacgata
     9241 tgaaaaattc gcggcaaccg gagtaagaaa tatttctggt tacaatgaat ttgttcaaca
     9301 gaaaaacttg gaaaacggaa cgaaacatcc aaccttacca ttcatcgtag tgatcgtaga
     9361 tgaattagct gatttgatga tggtagccag caatgaagtt gaagatgcga ttattcgttt
     9421 agcgcaaatg gctcgggcag cggggattca tatgatccta gctactcaac gtccaagtgt
     9481 ggatgttatc acaggaatca tcaaagcaaa tgtaccttcc agaatggcat ttgcggtatc
     9541 aagcggtaca gattctcgga cgatcattga ttctaatggg gcagaaaagc tcttagggcg
     9601 aggagatatg ctattcttgc caatgggtga gaataaaccg atccgtgtgc aaggagcatt
     9661 catttctgac cacgaagtag aacgggtcgt tcaatttgtt acagatcagc aagaagctca
     9721 ttatgaagag aaaatgatgc cgacagatga agttgaaacg acaggtgctc cagaacagcc
     9781 acaagatgag ctgtttgaag aagcaaaagc acttgtcgtt gaaatgcaaa cggcaagtat
     9841 ttcccttctc caaagaagat ttcggatcgg ttacaaccga gcagcacgtt tagtagatga
     9901 actggaagct catggagttg ttggcccgtc agaaggaagc aagcctcgga aagtattcat
     9961 tgagcaggaa gaaacatcag aaacgttgcc ggacgataat gacatgtaaa acttgtgtga
    10021 tgaagggttg aaaaacgaat aaccaataaa aagacaaggc atgatgatac caaaagaaaa
    10081 aatggttttt ttccatcttt tctaatacgg tatgatgtct tgtcttttta gtttttgtga
    10141 gattaaagtt ctattcgatt tatttgtgaa gtattgcatt aaggaaatga atcatgtata
    10201 gtatgtagcg aaactttgag aaaaagaggg aaaatacttt ggaaaaacag tccatttatg
    10261 gcttgacgaa tgaagaatta atcaattggt ttatagaaaa cggcgagaaa aaattccgag
    10321 ctgcccaagt ttgggaatgg ttgtatcaaa aacgtgtaag taatttcaca gaaatgacga
    10381 atctatcgaa acaattgatc gaaaaattat cggagcattt tatcatcaat cctctaaaac
    10441 agatggtcgt ccaagaagct tcggatggta cagtaaaata tttgtttgaa ctccctgata
    10501 aaaacatgat tgagactgta ctgatgagac aagaatatgg cttgtcagtt tgtgtgacga
    10561 cacaagtagg atgtaatatc ggctgtacgt tttgtgcaag cgggctgctc aaaaagaacc
    10621 gtgatctgac tgctggagaa attgttgcac agatcatgat ggtccaacat tattttgatg
    10681 agcgcaatct tggcgaacgc gtttcacatg tcgttgtaat ggggatcggt gagccatttg
    10741 ataattacga caatgtaatg gactttttgc atatcatcaa tgatgccaaa ggattggcaa
    10801 ttggtgctcg gcacattaca gtttccacta gtggattagc acacaaaatc aaggaattcg
    10861 ctaataacgg cttgcaagtc aatctggcta tttccttgca cgcgccgaat aatgaggtgc
    10921 gaacatcgat catgcggatc aaccgaagtt tcccaattga gaaattgatg gaagctgtgg
    10981 atgagtattt agagaaaaca aaccgtcgaa tcacgtttga atatatcatg ttgaatcaag
    11041 taaatgatag accagagcat gcgcaacaat tagcagatct attaaaagat aagaaaaaat
    11101 tagcatatgt caacttgatt ccatataatc cagtcagtga acatgatcaa tattctcgca
    11161 gtccaaaagc tgatgtattg agattttatg atgtattgaa gaaaaacggc gtaaactgtg
    11221 tgatacgtaa agaacatggt acagatattg atgcagcttg cggacaattg cgaagcaagc
    11281 aaatgaaaaa aacagttgcc aaataatcgt atcctactcg ttttttcaat taccataaaa
    11341 ggagcagttt tcttaattaa ggaactgttc aacttaaata agagaaaaga ttctatagcg
    11401 aaagctataa agcttttttt gacaagaaaa accctcatca agcaaagaaa tgtcctcaat
    11461 ttgatgacag cctgcttgat gggggtttta gcaattattt aactgatttt cttatatcct
    11521 tgctggcttt cttgtgtagc atgataagtt tctacgtcaa gaatggcctg tttaaatttt
    11581 tctcgggtga tttcataagg cacatgttta atatcatttg ttgccatggc tttatctaag
    11641 attgcttcta aatcggtcgt gacattgaga ttcatgtcgg ttaacgatac tggaagttgg
    11701 ttctttttca taaacacata gattttatca aacaaatcga actgctgatc catcatcaat
    11761 aaggtcagta caccataact gacagatgct ccgtgaaggt atccctccat tttttctaga
    11821 acagtcgtac cgtaatagaa tgagtgtgcc atagcagaat tatagttatt gttaactaaa
    11881 acagaaacca atccggtagt aacaatgata tccgaaacga cttgttcaat tgcatatgag
    11941 ggacgatcgt cggcactatc tttcaatgct tgcgtaccat aagtcaagag acgatcaatg
    12001 cacatttttc cgatttggat tcctaatcta tccgtgtagc ctagatcatc gccacgagca
    12061 gaaaaagtag attcgacttc tttggataaa gcatcgccaa tacctgccca taagtattta
    12121 tcaggtgctt cggcaataat tgctgtatca atgaaggtgt gtacaggtgg ttttgaagga
    12181 aagaagagtc cgtccattac gccttcttcg ttatagatga cacataccgc agtgacgggc
    12241 gaacagttcg aaccaatagt gggaaaggtg aagactggtt tagctagttt ttcaccaatg
    12301 acttttacag tatcgcaagc acggccacca ccgactgcaa acaggacgtc tgctttttgg
    12361 acactttcat gctggatcaa ctgttcaata tttccataag ttgaattacc cccgtaccag
    12421 agactggcga caacctctat ttcctgtgtc aaagctactt ttattttgtt ttctgccttt
    12481 gaaagtgcag tcttccctcc aatcagcact acttttgtcc catattcttt gacgattcca
    12541 tttgcttttt catacgcatc tgttccaatg gtataatttg gtaagaaaac actttgactc
    12601 atattcattt ccttctttct tcgttgaaac gggttaaaat tttaaagagt gatcgatctg
    12661 actgcggctg tagatggagt agaggtgaac ctaagacagg ataagtgatg aataaggtgg
    12721 catgaacacc ttgaaatcat caataagtat cctaaaaacg aacaagtaca aaaacattga
    12781 aaacgacaat aatgaatttt tgttcctctt agtatttcta ccgattccct ttgctttttt
    12841 gtaattatct aaaaattcta aaaattaaga ttattatatg aaaacaggtt ttatttgtca
    12901 attcataatt ggtttttttt aaagcaaaag ataatctata tcatgatgaa caaacagagt
    12961 tttttcctcc aattgatggc tttcctgatc tttcctattt tgaataaagt gtttctgtat
    13021 aattttttat tttataagta agataaataa tgaaagaata gagatactga ttggtagcta
    13081 aagatttttg agcgagaaca tttcgttttt tcaaaaggac tgttagaata agcaagaaca
    13141 agggggaaga cgtatgataa tcagtgaatt tgaccgtaat aatccagtat tgaaagatca
    13201 gctttctgat ttactgagac tgacttggcc ggaagaatat ggagacagct cggcagaaga
    13261 agtagaagaa atgatgaatc cagaacgaat cgcggtagca gcggtagacc aagatgagtt
    13321 agtaggattt attggtgcaa tccctcaata cggtatcaca ggttgggaat tgcatccatt
    13381 agttgtagaa agctcccgac gaaagaacca aataggtact cgattagtca attacttaga
    13441 aaaagaagta gcttccagag gaggaatcac gatttattta ggtacggatg atttagacca
    13501 tggaacaacg ttaagtcaaa cggacctgta tgagcataca tttgataaag tggcttccat
    13561 ccagaacctt cgtgaacatc cgtatgaatt ctatgaaaaa ttaggttata aaatcgtagg
    13621 tgtcttacca aatgcaaatg gctgggacaa accggatatt tggatggcaa aaacgattat
    13681 tcctcgacca aattctcaat aataaaaaga ggttgtgaca gaagtaatca gcttcaagaa
    13741 ataagaagga atttacgaaa attacttctt gaatttttgt gaaattccaa cttatttccg
    13801 aagaagctac ttttggaaca ccgtgtac
//
LOCUS       DAZBTF010000072        13792 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000072 DAZBTF010000000
VERSION     DAZBTF010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.072"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            112..306
                     /locus_tag="ACHGNS_002260"
     CDS             112..306
                     /locus_tag="ACHGNS_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938183.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            575..874
                     /locus_tag="ACHGNS_002261"
     CDS             575..874
                     /locus_tag="ACHGNS_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938184.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(971..1405)
                     /locus_tag="ACHGNS_002262"
     CDS             complement(971..1405)
                     /locus_tag="ACHGNS_002262"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6938185.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1637..2347)
                     /locus_tag="ACHGNS_002263"
     CDS             complement(1637..2347)
                     /locus_tag="ACHGNS_002263"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFN6938186.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2337..3197)
                     /locus_tag="ACHGNS_002264"
     CDS             complement(2337..3197)
                     /locus_tag="ACHGNS_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFN6938187.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3202..3840)
                     /locus_tag="ACHGNS_002265"
     CDS             complement(3202..3840)
                     /locus_tag="ACHGNS_002265"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFN6938188.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3855..4154)
                     /locus_tag="ACHGNS_002266"
     CDS             complement(3855..4154)
                     /locus_tag="ACHGNS_002266"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN6938189.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4197..5681)
                     /locus_tag="ACHGNS_002267"
     CDS             complement(4197..5681)
                     /locus_tag="ACHGNS_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFN6938190.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5701..6162)
                     /locus_tag="ACHGNS_002268"
     CDS             complement(5701..6162)
                     /locus_tag="ACHGNS_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN6938191.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6180..7247)
                     /gene="ulaG"
                     /locus_tag="ACHGNS_002269"
     CDS             complement(6180..7247)
                     /gene="ulaG"
                     /locus_tag="ACHGNS_002269"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFN6938192.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7465..8235
                     /locus_tag="ACHGNS_002270"
     CDS             7465..8235
                     /locus_tag="ACHGNS_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFN6938193.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8508..9335)
                     /locus_tag="ACHGNS_002271"
     CDS             complement(8508..9335)
                     /locus_tag="ACHGNS_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6938194.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9903..11138
                     /locus_tag="ACHGNS_002272"
     CDS             9903..11138
                     /locus_tag="ACHGNS_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFN6938195.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12137..12397)
                     /locus_tag="ACHGNS_002273"
     CDS             complement(12137..12397)
                     /locus_tag="ACHGNS_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFN6938196.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12424..13725)
                     /locus_tag="ACHGNS_002274"
     CDS             complement(12424..13725)
                     /locus_tag="ACHGNS_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN6938197.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4434 a         2828 c         2173 g         4357 t
ORIGIN      
        1 aaaactttca aattgattct ttttatttat cataaagata tagatacata atcattttca
       61 cttacatata tttgaatatt aactaaaaac agcaagaaag aagaggatca aatgtattat
      121 gtagaagtaa aaacaaaagg cgtcaaaaac aaacaatatg tgaaaggtat cagtaacgaa
      181 tatcctttac ttggttcatg gaaagaagct gctccttttt ccaaaccatg tgccataaaa
      241 ataaagaacg agctagaaaa agaactgact tgtggtaaag ctgttgtaga tatcatcgaa
      301 aagtaaatcc aatgtataga aaggaagatc acacatgaaa agcgacgtac aaattgctaa
      361 gattatagaa ttagaagaag aagacgcagt gttgacaatt gaaaaaaaat aaatgtttgt
      421 ataccatatg attatttcaa tatgtatcct ggaattggag atctcgtaga gctatatcaa
      481 gatgaatcaa atatacttgt cattcatcat gaaatataga gacttataaa attgtttatt
      541 tttataatgt atcagtatag aaaggaaata aaacatggat tatactgttg aatttgacag
      601 tgaactactt aagcaatttc cacaagaaga acatttcatg tataaagaag caatttccca
      661 caaagtgaca gagtatttgg agcataattt ttttcgtata aagccagtgc gtactcgttc
      721 ggcaaaagga atatacgaga tgaaagtcaa aattaataaa aaaaattatc gcatagcatt
      781 tgctttaaaa aaaaatcatg tgttcatttt ttatattagt tcgaatttgc aaaaaattgt
      841 atttgataaa gaggtgagta aaaaaataaa atagctgagc attggaggat agattgagtg
      901 atactaagtc tatcctcttg tttttctaaa gaactaagct agaatttttt cttttgtacg
      961 ttattatatg ttatttgtca ttaccaaaaa gcaccttttt tcttctagta tctatgacat
     1021 aagaaatacc acccatttcc tttacttgaa caaatcctac tgattcataa aatttttttg
     1081 ttttctcagt atcatctgtc aataattcga tttggactgg tccataacta gtgatcttct
     1141 ccaaagcttc attcaaaagc actttgccta ttccttggcg ctgatacctc ggatgaatga
     1201 ggatatcttg aataaatgcg atcgtcgcaa aatcagtgat ccaacgaacg attccgataa
     1261 tcttcccttc cccattacgc tttatcagag attcagattg ttcaaatgct ctagctaacc
     1321 ttgcagtgtc ttttgtgtaa ttcgtccaac caacgctctt atataattca gaaatctcat
     1381 cgatcataag ggaatcaata ttcatttttt tcttcctttc ttatacaatt ctccctctaa
     1441 taatacagtt ctttgactat ttaatctatc acatataact ctcctgtaaa gattctctcg
     1501 attccttaaa attcctcttt ccttttagaa tgcatacttt ttgataaata aaaaggttgt
     1561 gataaatttt cttcacaacc tctgtttctc tctatcaagt gttatctttt aattttctac
     1621 ccttacccta gcatctctat tgcccgtagt atgcatactt tccatgtttg cgaaaataat
     1681 gtttatctag taaatattgc gggatttcac atatattggg gttgatcagc tcgttttctt
     1741 tggccatcag acaaatttca tctaagatca aactgttttc tactgctttc ataggtgaat
     1801 ccccccatgt gaacggtcca tgtccataga caagtactcc agggacttcg tttggatcta
     1861 attttctttc cttaaacgtc tcgacaatca cattccctgt atgaacttcg taagcttctc
     1921 ttacttcttc tttcgttaat tggcgtgtac aaggaacctt accataaaaa gcatcagcat
     1981 gagtcgtgcc ataagctggc aaatcgcgtc ctgcttgcgc ccacatgacg gagtgcgtgg
     2041 aatgagtatg agtaatagca gttatatctt caaaagtttg atacaaaaca acatgtgtgg
     2101 ggagatctga agatggtttt aagctatctt cttctattat atttcccgac aaatctgtta
     2161 ccaccatttg atctgcttgc atgcattcat atctgacacc gcttggctta atgacgatga
     2221 ctcctagctc acggtccact tcactcacat tgccccaagt caatttgact aatccgtatc
     2281 gcggcaatgc cagatttgct tcaaggacac gttgcttcat tgctgcaatt ttttcttcac
     2341 aagacatcat agccagcctc cttcaattta gaacttaagt agtcttttgc ctgctggatt
     2401 tctcttcgaa agtcttggct tttttcactc cacatttcaa tcagaaatgt tccatcataa
     2461 tctagacgtt tcaaatgtct taataatcca agaaaatcaa cacaaccgct accaaaagga
     2521 acatcacgaa actggcctga atattctttt gttacagggt aagtatcttt taagtggata
     2581 gcagtgatat aatcgatccc tttctcaagt tctcgagccg gattattttc cggccatgcg
     2641 gacaaattgc ctaggtctgg atatacttgt agaaaaggtg aatgaatctg ttctttgatt
     2701 tctaaaaatt ttgaaatgga gttcataaat ggatcatcca tgatttccac ggaaagaatg
     2761 atgccatatt tagaagcttc ctttacgctt tcttttattc cttcgataaa gtcttctctg
     2821 gaattcattg atttctcttc ataataaaca tcgtatccag ctatttggat tactttgacg
     2881 cttagattat gcgccaaatg gattgctttt ttcattagtt gtttcgctgc ttttttcttt
     2941 tctggatcag cagaaccaaa cggaaatcgc cgatgcgcac ttaaacaaat cgaatggata
     3001 cgtacaccgg agctgaacat ctcttctctc agctcagcta tcttttcttc agaccaatct
     3061 aatcgtgcta accgctcatc cgtctcatca atcgacatct cgataaaatc gaagtccatt
     3121 tcttttacaa gggaaaaacg ttctttccat gaaatatctt tgggtaacgc tttttcataa
     3181 atcccaattt gagccatttc cttaccccca gatacgaatg atctcatctt tgaagttttg
     3241 tgcggccttc atcggatcat ttgctgctgt aataccacga ccagtgataa aggtatacac
     3301 atcgatccct tcaaataagt gtaatgtgtg tggatttaat ccaccagtta cagaaacatt
     3361 aaagcctagt tcaatcagtt ttttgatctt cgataaatct ttttcacccc aagtttcacc
     3421 agataacagc gcatcacggc tttgatggta gattacctgc cggataccga tgttatgcca
     3481 atccatggct tgttcaaaag tccaattgcc gtagagttct acttgcaatt cacctatttc
     3541 tttttgtgct gcctccatag ttggaatcgt tgcacaacaa atgactgtca tccagtctgc
     3601 tcctgctttt gcaacattac tcgccactgt tcctccagcg tctgcacatt tcgtatcagc
     3661 gactagtttt ttatctggga acatactgcg aatacagcga atcgcttttt gtccttcctg
     3721 taagcaaaga attgtgccca cttcaataat atctactaca tcaccaactt tcataacatc
     3781 tcctaatgca tgttctaagt cattgtgatc taacgcaatt tgcaaattcg gcctgctcat
     3841 cttagttcct tcctttactt gtttttttgg taccactttg tttcagcaaa tttttcactg
     3901 atttctttga ctgacatcac atttttgata ccgataactg taactccttt tttctctgcc
     3961 tgagtaaaca tattcaaaaa attgactggc gtaaaaacta catcgtattg gccagctgaa
     4021 ctttttccct ctgaaatggc acaatgatgg attttggtaa ttggtacgtt tagttctttc
     4081 attgcttttt ccacacttct catcatcatc aagcttgttc ctgatccatt tgcacacgat
     4141 actaaaattc tcatcttaat ccctccatta tgagtgttgt ctaatcagat tttcttttat
     4201 actctctgct tttctgcata tgtgtcatag tcttctacaa tcaaaaaata accatcagga
     4261 tttttccgat attgtaactg cggaatagcc aataaaccaa tgacaactac ggcaattcca
     4321 gcataaccta ggactttcat gattactgta aacaatggcc aagctgttgc ccagtcgaac
     4381 atcccgatat aaccaccgta tgttgacatc ccaatccatg tagcgaataa tgctgaaccg
     4441 cctacttgta tgattcctga aagaaacggg aacaggcaag cggctttgaa tccacctcgg
     4501 ttattggcat aaactgctat aacggcatta tcaaagaaca acggaatgaa accggctatg
     4561 acgatcgttg gtgatttcaa taaaatcagt aaaccaatca tcaaaaactg tcccaaagca
     4621 ccaaacaaga acccaatcgt taccgcattt ggcgaaccaa agccaaaagt agcagctacg
     4681 tcaattcctg gaacagctcc tggtaaaatc gtgtttgaga ttccttggaa agattgagtt
     4741 agttcatcga cgaatgtccg aacacctaat tgcaagatcg ccagatatac cgcaaaattt
     4801 aaagaagtcg tcataatgta gaataagaaa ctctgccctt cttgcatgaa ttgcgcttga
     4861 atcaaataat ctttgcctag tacaatcaga atgatcccaa agaaaaatag catgagtaac
     4921 gaagtagcaa ccatattctc attgaaaatc gacaagaatc ctggtaattt gatatcttca
     4981 atttttttgt cttgcttgat tgatctattt ttcttttcat cccgcttttt catccactca
     5041 gctaattttg caaatataaa gattccgaac atttgctgat gtgccactgc aaatccggca
     5101 ccttctgtca attcctgcgt gattcctact gttaagttag aagctactgt ccaataacaa
     5161 cctaaaatca gtcccataaa aatcaatgct tgtatcctac ctagatcagg aaagcaaaac
     5221 agtaaaatcc aaaaagcggt agctgcttgt tgaacctgaa cgtttccggt agtgaaaacg
     5281 gaacgcagtt tcgtatattt ctgaaaacgg actaataaga tatttaaaac aaatgcaatc
     5341 agtaataaaa tcatcacatc tccaaaggta cggccaaatg tctccattaa tccagcatcc
     5401 acagcatttt gtccaaaata aggatctgta accatggcat ccaaattgaa ccgttccttt
     5461 agtccaacca agattggccg aaaattattg accaaaccac cagagccaac agtcaaaata
     5521 aagtaaccga ccgttgcctt taagaatcca gcgatgcatt catgaagcgg gcgtttcagc
     5581 aaaatatagc ctaataacac gataaaacca atcaggtatg ctggctgcgt caagatattt
     5641 gttgcaaaat acgtccaaat gcttaacaaa aattccccca tcttttttct cctctatcaa
     5701 ttaattatat tctgccatca caacttggta atcatcgatg gagttcgtag agatgagacg
     5761 ctcaaccaat ccatctgtca ttaataattc ggataatttt tggatatttt ctagatgttc
     5821 ttctggatct tttgctgcga gtgtaaaaaa caaactagcc gttttctcct cattctcccc
     5881 ttcactttca aaacggattg gctgtttcaa cttggtaaat gaaatagctg ttccaaaaac
     5941 accatcactt ttttctgaag aatgcggcat tgctacttga ggtgcgatca caatataagg
     6001 accgtatttt tctacacaat ggatgatctc ctccacatac gtttcatcaa taatttcctt
     6061 atcgatcaat acaagacagc tttcggcgat agcttcacgc caatccgtga tttctttatc
     6121 tgggtaatga atcaattgat ggtcaaaaaa gtatttcagc aagaaactcc ccttctttct
     6181 tataaaaacg acggaaacgg tgtatcattt tcaatcgaga agacatcttc aaatcctcga
     6241 taaaaattaa actcaagatc atctttgttt gtcggataga tgaatttccc gcctacttgc
     6301 cagatataag gtttaaatcc atacttcagg cgatcctttt tgaatttcca aatttcagtg
     6361 atctcttttg gatcagccat aaaattagac caaatatcgt agtggacagg aatcactact
     6421 tttgcattca atgactccgc catgcgcagc atatcaacag aagtaacttt gtctgttatc
     6481 cctcgtgggt tttcaccata ggcccctaaa cagacatcga ttttgtgttc attaccatgt
     6541 ttggcaaata aattggagta atgtgaatct cctgcatgat aaagattccc tcctgatgtt
     6601 tcgaataaat agtttactgc aatttgatcc atctcctgag gcatttttcc ttttaatacc
     6661 tcttctggat cttcacaagt gaccaacgct gtccgatcaa acgcttctaa agctactaca
     6721 ttgatgtctt tgattttgac tgcatcacct ggctttactg taatcgtttt ttcttcagga
     6781 attccccatt tttgccacgt gcgaaccact tcttttggcc caatgaatgt tgcttcagga
     6841 caattttgat gaactgctgc ggctgtatgg atatctagat gatcggaatg gatatgtgta
     6901 acgattaaag cgtctacatt ttttatcgca aaaggatcga ttacgaaagg ctgagtacgt
     6961 aaattaggct gcatattttg aacgccactc atacgcatca tctgatggcc ttttttcatc
     7021 atgccattcc catgactacg ttttcccgtc ccacaccata gatcacataa aatattcgtt
     7081 ccttcatgac ttttcagcca aattcccgta cagcccagcc accacatagc aaaagttcca
     7141 ggatccacta cttctgtttc gatttcttca ttcaagtatg tgccccactc aggaaatgtt
     7201 tctactaacc acttttcttt tgttacttca ttgacagttt tactcatttg cttgctcccc
     7261 ccatatcgtt gttccaactt acaagtaaat attaatgtgt atcagcatcc atgaaaaccc
     7321 ttttatatga ccgtttgttt tttatatgat tttctttcag taatcaatga ttgtttcagt
     7381 ataataaaga cagatatgaa tgtttaaatg aacaactagt caaataaatc catggatcaa
     7441 gatgtccctc aggaggcaaa agtcatgaaa aactcacttg tcaatattga aaaacgtcaa
     7501 aaagaaattt tggaactgct tcagcagact gaacaactat caactttcga attagctgaa
     7561 tcccttgatg tatctattag tacgatacga agagatttaa atttattaga agaaaaaaac
     7621 gagatcatac gcaattacgg ttattgttct tataattaca aaaaccagac tgattttgat
     7681 caatctggtc ctgaaaggat taaacaagcg attgccagag aagctagtaa atacattaac
     7741 gattatgata cgctgtttat caattctagc tcgactgcgt tgaaaacact ggattttctt
     7801 caatctaacc atttgacagt cattacaaac aacgtgaaaa tcaattactc tccgcacaaa
     7861 gataattgta gttatatttt aactggtgga gagatgcgct ttccaaaaga agcactagtg
     7921 ggcgacatcg cattaaatac gatttcttca gtcaatgcag acatttgtat cattggctgt
     7981 agcggagtcg atgtagtaca tggggtcaca acaaagatat taaatgaatc aacgatcaac
     8041 gaaatgatgg tcaacaagac aatcaaatgt aagattttag tcgctgatca tcgaaaaatc
     8101 ggactgacat caaagttcaa gattgctgat atttcagtct ttgattattt gatcactgat
     8161 aaattttgtt ctgaaaaagt attagaggag atcagaaata cggggataaa aaccattcaa
     8221 gtagattcat tttaagaacc aggacgtaaa tatgtaaata ggattatcaa aaaaatgaat
     8281 aacaaattta tcgataaatg aaaaacacgc tacgctcaaa ttgataaagg aatcccaaga
     8341 tgtatccatc caatcgatac catcataatc tattgcttct tttataaccg ttatatgttg
     8401 cctaaatagg aatcaaacaa tatcgaaaaa aggaggtatg acaaaagtca cacctccttt
     8461 tttcgaataa acggtattca aatcagaaca ctgatttcac aaccacttta tttcaggtcg
     8521 ttttcaatca cctttaccgc gtaatcattt ccgttgaaac gcgctatttt ttttgcgtcc
     8581 atatatcttt ctaaataatc atcttctcct aattgcttat ggcaagaagc aatttcataa
     8641 agcaagtatt ccaatgcata agtcgattta tgtttcttat tatactcaat acccttctca
     8701 cataattgga ttgctttatc ataatcctcg attttacggt aataggtggc gatatcataa
     8761 tatacaatgc agaccatcag attatcaaaa tcagtctcac tcaatatttt aagtgtacgc
     8821 tccacataaa ctttcgcaaa atctaattct ccttttgaca agtagatcat tgccacgttt
     8881 gcagctgctt gtaagtgatg gatatctgtt tcggtcgttt gttccaacac ttgattaaaa
     8941 taaaataatg ctgcatctgg ttttttcgca taaagatgat caagcagtcc ttgataaaat
     9001 aaaatttgat ttaaaagatc agaattactt actttttcct gatcgatcgt ttgtaaaatt
     9061 ttataagatt cttggtattt tgatacagaa ttcaatgatt gaatctcatt tatttttttc
     9121 ctgattttgt cttcttctgt ttctactaat atatcggaga tctcgacatt cagctttttg
     9181 caaagcgcac ttaagatatt gatgcctggc gacatattct tgttttcgat attgctgaca
     9241 gttgcttgtt cacaaatgcc ttctgctaat tgtacttgtg ttaacccctt tttctttcgg
     9301 tattctttta gtaatgctcc atctacttta atcaaaacaa tttccttccc tttaaaaaat
     9361 tatttctcct ttatatcata cacaaaaaaa agttctaaac aaccaaaaat gtttagaaca
     9421 gaataaaaat tttaatttta aaataatatt caaattaaac aactggcgta tctgctcctt
     9481 gatttctttt tgtgttatct gaataattat cgtttacagt tataacacat agtgaaaatt
     9541 gtcatggcga gaaagtctaa gaatattttt aaacataact atttctttct tacgtgtgta
     9601 taatgacgac agcacaccta tcaattaata taattatttt atttttttat gttctcatgt
     9661 ttatttttaa tgacacaact tctcgtattt atctggcaca acatataaat cgatcaaaaa
     9721 attgttgcgt gtttttttga aaattggagt ttcaacttga ttacaaaatt taaatttaga
     9781 agttgtgaaa acagcgagag acagtttaat attcaaaata tacataggct actcgaacgc
     9841 tgtgtttttt agtatactag taagaaacga agggatagac tcattccaat aaggaggatt
     9901 tcatgaagac aattcttttt gctcctgcag tattcaattt agctgagaca acacgaatga
     9961 ttgaggttgc caaagctatg aaagaccgtt attactgtgt attctttggc ttttctaaaa
    10021 cttttgctcc tttgatcaca gaaagtggct ttgagtatca tttactagcg ccgatcctta
    10081 cgaaaaaaca agaagaacaa atcataaaat ttgaccagtt gcgtacactt aaaaaccctt
    10141 ttaccaaaga aatggttgcc tctcgtatca ctagtgaaca gagtctgatt gctaagtttg
    10201 agcctcgact gattgtgaca ggttcgaatg tgacaatttt tttatcggcc cgcaataaaa
    10261 agattccttt agtatatgtt aaaccctatg ctttgagtga accttttttt caatcagagc
    10321 atgcgctgat gccccacgag ttacgtttct tcggcccttt aagcaatccc ctttggcact
    10381 ggacaaaaaa gcgtatagta aatacccgat ggatccccaa tgccttcaaa cacgctgcac
    10441 aaaaagaaat gcttccttta ccaaaaacaa gtttacaaat gatggatgct gatctaaatc
    10501 tcatcacaac gcctaaacta tttttggaag aagaacagct gccggaaaat tatcgaatcg
    10561 ttggtccaat ttttgctaag ctcagtactt ccttgcctga agaagtgcta acttttatac
    10621 agcgaaagca acaagaaaga aaacgtatta tctatttcgc aatgggtagt tcaggcaatc
    10681 cgcgtatgtt acgcaaaatt ttgttcttct tacaaaaaca accggatcta gcggttattg
    10741 cacctattga ctatctgctt gatgacattc aatctgactc agaagatctt ttaataacag
    10801 aatatctgtc tagtcatctt ttgcatgaat tcattgattt ttcttttatc cacggcgggg
    10861 aagggaccgt ccaaactgcc tgtgaatccg gcaaaccgtt tatcggtgtg gggatgcaat
    10921 ttgagcaaag ctgtaatatt gactattgtg taaaatacgg aaacgcaatc gccctcacaa
    10981 aaccagtgac tgaaagaaaa ctgcagattg ctctgcgcga agtttcttca cccaagattc
    11041 gtcagcaggc acgcacgctg caagccgttt ttccaataaa tggtccacaa caagcaattc
    11101 aagaaatcga aaccttcctg agcaaccacc caatatagtt tcaagtagaa aaagcttttt
    11161 taatagagac tcatcagtaa tttcgctatt cgagacccag acagtgaaaa tacaaaaaaa
    11221 gtaaatagtg gattgaaaag ttagagttac ttaaattacg gtgtctaaaa aacacaaaat
    11281 catccttaaa ataaaagatc tgacgctatc caaatgagca taggtagcgt ctgccattta
    11341 ttcttagtca aattctttga cacctttctc tggttggatc atctcaacgt ttcttcaaaa
    11401 gaaagaccat aataattagg caaatacatt ttcttaaaat gtgtcatata gatctagaag
    11461 catcgctttg tagagatttg tgccgctagt cccctcaaca attactaaat caaatgtagg
    11521 atttaccact ttatctttga taatcaacct ccctcttctg ttgagacaat gatctcgcaa
    11581 tccgatgcat actcctatat aaactgccag caatccccac atattgagta gatcatgtca
    11641 gaatgttcta tgtcgtcagc aacgatacac taaaagtcac gttcaccatg actgattgct
    11701 attcccagca caattatttc tatacacgtt tcatgatttg aataaacact cacatcatac
    11761 atattaccat tcatacacca tactgcagcc cctactatat ggttgtaagc tcccaaatct
    11821 atatttgact tgatcacttt ggtagtagat ctactaattc taaatccatt gctgataaaa
    11881 tttccatttt ttcctccttc tttctgccaa aacagttaaa acaataataa tctttatgta
    11941 aaaaagggct gtgacaaaag ccttgtcaca gtcccatatt ccgaataaac ggtggtcaaa
    12001 agcttgcatc tgcggtaata agttcaacca aacgaaaaat aagccaacat ttcacaaaaa
    12061 tttaagaagc aattttcgta aatttcttct tatttcttga agctaaccac ttctgttaca
    12121 accactttta gaacatttaa agcttcgatg tgacaaacca tctatgctgc tcgatcataa
    12181 aattgatagc aaaaattcca caaagtacat atgtccccca tgaaaacaaa acattcatat
    12241 gcggtacaat gatctttgga agaatcacga tcaaaaatga tgccagtgcc caaatccatc
    12301 gattgatttg aatatgatat gtagctagca tattcaatat aaggtgacta gccgttaata
    12361 aaaagataaa aacaaaaaat aaaatcagcc aatacattgt ttcccacctt ctaaaaacca
    12421 aacttagaca gtctggactt cgttttctcc acgactttgt tcatctgaca gcatcttttt
    12481 atcataagct tttacaaatg gataatagat aataaatgct atcaatgtgt tgatcatgat
    12541 caagataaca acacgccagt catttcctgc tgaaagataa ccgccgattg gagctggtaa
    12601 tgtccatgtt ggcaaaacag taaatctatt gaccatttct aatttgactg caaaataact
    12661 gataatagtc aaaactaaag gaactaagtt gaacgggatg aacatgtaag gattcaacat
    12721 cataggagct ccaaaaatta tgggctcatt gatattgaag ataccaggaa taatagaaaa
    12781 acgaccaacc tctttcaaga attttgattt acagaagaat ataaacaaaa cagataccac
    12841 gatcgttgct cctgcaccac caattgttac cgtccattga taaaactgtt caggagcgat
    12901 atagggtaat tgggtggcag ctgtaccatc tgccaatgcc tttgcattct catccaacat
    12961 gatcagccac attggacgaa caacagcccc tacaatactc atgccatgga taccaaatga
    13021 ccacaacaaa tggatcaaaa acattggtag taaaacacca agcaaattat tccctaaaat
    13081 attggttaaa ggctcgaata acgataataa ggcagcgttt acatcaaagc ccgcgacgct
    13141 tcgaatcagc caagtaattg ttaaaatgac tgcacctgga atcaatgcct caaaactacg
    13201 ggcaacagca ggtggcactt gttctggcat tttgatagta atatttcgat ttttaaagaa
    13261 acgatatact tccactgcaa aaatcataga taagatcccc gcaaacatcc cagatgctcc
    13321 caaagaactc ataggtaaga cccagccaag aggtgcctct tcaggagtta aaacgtttac
    13381 aggaacttgt aacataaaga acgttcctaa agataatata ccacctgtca cactatctaa
    13441 tccatatgat ttagctagat ccgaacccat aacaaaagtt gcataaatag ccatcaatcc
    13501 catcgtcatc ctaaagggta aaacaatcgt tgccgaataa ggggcaataa attcattcca
    13561 tccaggaaac ggtaaattga taaagaccat aaaaaatgtc ccaatcaaaa tcaatgacaa
    13621 tgttgaaaca accccattac gaattgctaa taaatgacgc tgtgtaccaa tttttgccat
    13681 tggtgggaca agtttctctt cgacccattt catcagcttt tccactaaaa ccactccttt
    13741 tcaataaaat atgaaccatt cattgcatga caacgcaaag aatgttcata ac
//
LOCUS       DAZBTF010000073        13357 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000073 DAZBTF010000000
VERSION     DAZBTF010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.073"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(573..1187)
                     /locus_tag="ACHGNS_002275"
     CDS             complement(573..1187)
                     /locus_tag="ACHGNS_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFN6938198.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1793..2170)
                     /locus_tag="ACHGNS_002276"
     CDS             complement(1793..2170)
                     /locus_tag="ACHGNS_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938199.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2470..3351)
                     /gene="galU"
                     /locus_tag="ACHGNS_002277"
     CDS             complement(2470..3351)
                     /gene="galU"
                     /locus_tag="ACHGNS_002277"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFN6938200.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(3394..4413)
                     /locus_tag="ACHGNS_002278"
     CDS             complement(3394..4413)
                     /locus_tag="ACHGNS_002278"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFN6938201.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(5046..6050)
                     /locus_tag="ACHGNS_002279"
     CDS             complement(5046..6050)
                     /locus_tag="ACHGNS_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFN6938202.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(6063..7112)
                     /locus_tag="ACHGNS_002280"
     CDS             complement(6063..7112)
                     /locus_tag="ACHGNS_002280"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFN6938203.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(7129..7950)
                     /locus_tag="ACHGNS_002281"
     CDS             complement(7129..7950)
                     /locus_tag="ACHGNS_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN6938204.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(7937..8716)
                     /locus_tag="ACHGNS_002282"
     CDS             complement(7937..8716)
                     /locus_tag="ACHGNS_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFN6938205.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(8733..9203)
                     /locus_tag="ACHGNS_002283"
     CDS             complement(8733..9203)
                     /locus_tag="ACHGNS_002283"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN6938206.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(9215..9634)
                     /locus_tag="ACHGNS_002284"
     CDS             complement(9215..9634)
                     /locus_tag="ACHGNS_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN6938207.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(9715..12492)
                     /locus_tag="ACHGNS_002285"
     CDS             complement(9715..12492)
                     /locus_tag="ACHGNS_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFN6938208.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            12683..12907
                     /locus_tag="ACHGNS_002286"
     CDS             12683..12907
                     /locus_tag="ACHGNS_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938209.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT         3926 a         2773 c         2156 g         4502 t
ORIGIN      
        1 taaaattctt tttttatttt tagattagag aaaatctcgt cctgtttttt tcattttaca
       61 atcattgaaa aattgaattt cattgcaaaa aaaataataa acacttataa atatccatta
      121 tctatccttc aatttttgtg tattttattt ctattagaaa aaggtatcct gttggacaat
      181 tttgttatat caggtaaact tcattgcaat aatgataaca aatgtactat aattactttg
      241 taaagagttt tggggaagac tctttcatca acgtgtatta agagacaaga aagaatccat
      301 atggattctt tcttgtctct ttttccacta ctttttattt ttctgacact acatattctt
      361 ataactcatt tctcataact gtgaaacaac ttataaaaat aacaaacttt aaacaaaaca
      421 agcttaaaat caaagatgat ccatcttaaa ataactaaaa caaaaaaagt agtcgttgtt
      481 aatcctcatt aataatcgtt ttcttttttt caaacgctcc atttttatgt ggaacattat
      541 ttttttaagg aactttgtca acatgtggat aactattttc cacttgtgtc ctggactagc
      601 tgttttgctg taaaatacaa aacttgctgc agcaaaaaga tcgttgcaag agaggctgtt
      661 ccgcagatcg ctggatctag atttggcaaa gtcaagactg catcatgtaa attggcgtgg
      721 ttgtccaaaa ccaaatcagc aaattcaaat aatcgcaaac cactgctatg ttgcgatttg
      781 atacttcgtg acaaatcaaa tccagtaaca atgattaatg gatgaccatt ttctttgatc
      841 cactgagcta gttcgatgat tgccgggttt cttccttcat tcgaaagaag aagaaaaatg
      901 tcttctgatt tgacagaaag ctgatcaata attgcctgtc ctgcaccttc tagcgtttca
      961 tagacacctc cggctggatt ttcaatcaat atacttggta caagatccgg ggtttgatcc
     1021 caaaattcaa atgctacacc acttagtgtg cggctagcat atatctgcaa tctaccacct
     1081 tgattaattt gtttcgcgat ccaagtgcca gcttgaagca tcagcggctc ttctatcctt
     1141 aatttctctt ccaattggtg ataatagtca aaataacata aactcattta tctctcccgc
     1201 ctagctatac ttgacatgaa tagaaacagc tgcctaatct aaggaagtac ataaaaaaac
     1261 agtagttaga cagtaacaca tgtggtcttt gctgtttcaa acacaaaaaa ggcattgaac
     1321 gaatacgccc aataccttga catagcaaat aaaacaacgt gcagaccatg gaaaatatct
     1381 acataggggt agaattttca ccctagatta ggtacaataa attaggtcga tgcactgcat
     1441 tgactatggc aaaatgatcg gtcagggtgt atgtgtgtga ctggtcattc cctgattctt
     1501 agcgccaact aagggtcagg gagccttttt ttattcagct gtttctatct gaatttattg
     1561 taagtaatct cttatccaag tgcaagcaag aaaatacaaa aaaacattgg tatgatttat
     1621 atatatataa ataacataaa aaaaggaatg gcttatcccg atacaacaag cattccttta
     1681 aaaaagattc ttttatataa gtgaaaaaag cttttttcca ccaattaaat aaatctctca
     1741 tctcaatagt ttatccatta ttttatcttt ttttgcgtat acctactaaa tattaccgtc
     1801 ttcttctcgt tcttctactt tgttcgagag aatctgtact agaaaaagta gttgtatgtc
     1861 taccttttct gcctatccga gactttcttt tcatatatgg gatataaatg attagataca
     1921 gtacagcaaa aaataaagca aatacgcaag cttcccaact acctaacgcc aattcaacca
     1981 tcaaggtagc aaccccaacg gatgctgcat accagataaa aatcagaaac gtttcttttt
     2041 ttctgaccat gcgaaacatg ataaatagac taataagcaa tagtggcaac acaaatatcc
     2101 aattgcttcc ttccatcagc cacttgagta tatcaagcag cgactgtgca acagcgacta
     2161 attttcccat agtgaacaca cacctttttt tataattaaa aaattcgcct ttttattcta
     2221 ctatgaatct tctggcttgg ctttcatttc tcttatgtaa tcattttctt ttatacaact
     2281 taaaaaaaaa ttaaaccatt agcataatat tttctgaatc tattcacgaa agtctttttc
     2341 tatctttcgt tttccacgac tcaactgatc tagagtcaaa aaactaaaac gacagttgcg
     2401 caaatgacgc acaagctgcc gctttaataa caaatagcct ttcattctca gcaggccact
     2461 tatttccagt taactttctg ttcttagctt tttactcaac tggataatat atgcacttaa
     2521 tgaagtccca atttccgaat gttgaagtcc atattcaata tttgtcttca tatatcctaa
     2581 cttatcgcct acgtcgtatc tttttccttt aaactctctc gcatagaccg cctctgtctg
     2641 gttaagagtc tgaagtgcat ccgttagctg tatctcgttt cctgctcctg gttcttgagt
     2701 ttctaatatc tcaaatattt taggagtcag tagataacgt ccgataatgg ctaaattact
     2761 tggtgctttt cccatttcag gtttttctac aaaccctttt actctgcaac gacccttttc
     2821 gctatctgtt ttaggatcaa taattccgta tttagatgtt tcattttctg gtacacgcat
     2881 gacggcaaca gcggaagctt gtgtttcttc ataatccatc atcaattgtt gagtcaatgg
     2941 gatatcatct ttcatcaaat catcccccag catgacgaca aacggttcat ttcctataaa
     3001 tgcttttgct tgaagcacgg catcccccag tcctcttgga tgagattgac ggatgaagtg
     3061 aagattgact tgagtcgttt cttcaactaa agcgagtaat tctgtttttt ccttttcccg
     3121 caaattgttt tccaattcga tatttgcatc aaagtgatct tcaatgggtc ttttttcttt
     3181 tcccgttacg atcaagatgt cttcgattcc cgaatccaat gcttcttcca caataaactg
     3241 gatcgttggt ttatccacga ttggaagcat ttcttttgcc atggcttttg ttgccggcaa
     3301 aaaacgtgtt cctagcccag cggcaggaat gactgctttt ttgaccttca cttgctgttc
     3361 ctcctaaacg acgattaact attgatcatc tatttaacga agagaaaatt cattctccat
     3421 ttttccgtca cgtaacatga tttcttttgc tgcttttttg atatcttctc cattatacaa
     3481 aacattatag attgtttgag taattggcat ctcaacacct agctgttgag ctagctctac
     3541 tgctgctttt gtcgttgcca caccctcaac gaccattccc atgttttcta gaacttcttc
     3601 cagcttatgt ccctgtccta acaagttacc ggcacgccaa ttgcgagaat gaacacttgt
     3661 acaagtaacg atcaagtccc ccacaccact caaaccgata aatgtcagag gattggcacc
     3721 catcgcgaca ccaagacggc tgatttctgc caatcctcga gtcatgattg ctgcttttgc
     3781 atcatctccg aatcctaatc catggatagc gccggcacca attgcaatga tatttttcag
     3841 tgctgcacca gtttctacgc caatcacatc attatttgta taaatcctga aataatcatt
     3901 catgaacagt ttttgtacat aggctgctgc tttttgatca atacttgcag ctgtaattgt
     3961 tgtgatatca tgtactgcca cttcctcagc atggctagga ccagataata caacaattgc
     4021 ctgtcttttt tcttctggaa tttcttcagc caaaacttct gagattcgtt tatgcgtatc
     4081 ttgttctagt cctttacttg catgtataat gattggctta gtattcattt taccaataag
     4141 ttcctgtgcg actgaacgaa tcgcttttgt tggaacaaca aataaaatag catctgcgtt
     4201 atccactgct tctgccaatt ctttatatcc gataatcgat tctgggatcg tcaaatcagg
     4261 taaaaaatgc tgatttgtat gatacttatt gatttcatca atctgttcag gtacatttcc
     4321 ccaaatacgg acatcatgcc cgttttctgc caatgtttgt gctaaagcag tgccccaaga
     4381 tccaggacca agaactgcga ttttttgttt catggatatt cctccaaata aataattgat
     4441 aatttccatt atagtataca tttcttaaag ttgggactaa tttccattgt ttttaagtgt
     4501 ttgtttactt cttttatcgt ttttacttga aataatcaat gatatgttac aatagtctac
     4561 attttatttt tacactcgtt atgctttgct tgttcctaag aggaagcagt tttgacaatg
     4621 ttactaccag cattactatt aggaggttag gaaacttctc ctagtgagta ttgatttaaa
     4681 atacataaga gacagattga acatttctaa atctgtcagg tcctgcaacg gacagattgt
     4741 ctctgttgac ggttgacata ggaacttttg ttttagtcaa cattctggat acaaaacgct
     4801 atgtaaactg gcgcaactcg ttgtacttgg acttttacta cggatccatg caacaactaa
     4861 atagtactat tgcataacaa aaaatggttg gcacccacat gtcccattaa acagattata
     4921 agttgaactg tagataatgc aaaaaatgtg ttttataacg taattgatgc caatatttta
     4981 aaaaaataaa gggagcagtt atcgctgctc tctttatttt ttatattctt tttatccagt
     5041 ctatcctaat aatcaaactg acgatagtag cggcgtatct ctaatggatg gcagttcaac
     5101 tgctccacat gagcatcgat gcgttgtgtc actacatgca tcaataagaa agacaaaaca
     5161 cgtccgcgat atttttcact gataccttct actggatagt cttttgtgtc tatcaatgta
     5221 taattgctgc aaatttttgg caaaagttta gccacacgtt cagataacac tctctgttca
     5281 tcttcgccta aaaataatgt gactggggtt gtttcttcta cgatctctaa cgtcccgtga
     5341 agaaactcgg cagcatggat agatttcgat ggaagccagc tttgttcttc ccagtaacac
     5401 atggcatatg aataaacagc tccccattga ttccctgctc caataaaata atgcatacta
     5461 tcacgtcgat gtttttctgc aaatgataag ccaaatgcat ctgctttttt ctctgcttcc
     5521 actaaccctg ttggtaaata acgttcgagc tgttcatagt attctgaata agcttcgaat
     5581 tctccattca aatacatcaa tcgatctgct gccataaaaa atttgatttg ttctgttcca
     5641 ggagctggat aagtgaccac aaaatcgcat agctgagaca acttactgtt cgctttatcg
     5701 ataaatccaa tcagtgtcgc tcctactttc ttgatttgtt ctactgcttt cactacttct
     5761 tgagttgtac cggtaacaga ggacaaaata acgatagaat cttttgttaa tcttttgttt
     5821 ccagtagtgt aatattctgc agcatgctgt acaaagacag gcaagttgct tttcccattc
     5881 atataagtga cagcttgcat cgaggaagca tacgttccac cgattcctag ataatagaca
     5941 gcgtcaaatt tttccgcata aagtttatcg atgatttctt ctacttgcgg acgtaaagct
     6001 agtgcgcctt ttattgcttt gatttgttct tcttcattga atttcagcat gatttccacc
     6061 tcttataatt tacttttcaa gactgtatca aagtcttcaa aaatcttttt gttcaagtca
     6121 atacctttag ctactgcggt tttataagcg aatagttgaa acggaacgac aagcgctaat
     6181 acagacagat attcatcaca ttctattgct aatccaagtg ttttctcgtt tcttgctttt
     6241 tttgtggtaa tggtgagagt ctctccaacg taccgactca tatatctttg caacttttgc
     6301 gaacgttcct tatttacact ttccccttca ataaagaata gcaaatgcga agcatctgcc
     6361 tccaggtatg ggccgtgcat ataagcttct aactcaaatc cttgtgacgg aacgcggatt
     6421 gtttcagtca atttcgtctc tgcttcttta gctgttcccc agtttggacc atatccaatg
     6481 gtgacgaaac gctgtgctaa acgaaataaa ctttgatatc catcaaaaaa ttcttccgtt
     6541 ttttgaataa tcgctggtaa atggttgatt attttgcgca actgttgcat gtagtcttgt
     6601 tctatttttt tgctgatttt atttttactt attccaattc ctatccctaa gagtagtaac
     6661 tgtaaaacag tcgcagaata gccttttgta acgaagccta ctgattctac cccggcacct
     6721 aaatcaataa tttgtgcagc ttctaaagca agtggacttt gcacgtcatt tgtgatagca
     6781 acaacaggta acccttgttt ttgtatcatt tttactgctt caacagtaga agcacttttc
     6841 ccgctttgtg acacagcaat cacagtatcg attgaaggag aaagttttcc ataattgaaa
     6901 aaataaaaag gttcttgaat atcgacagta agatcaccat aagattccaa tgctggcttc
     6961 gctgctaaac aagcgttata agatgaaccc gttgctaaga tcagtaaatt cactgttttt
     7021 ttcatatgtt ctcttgtcga aaaatcattc tttttcaaga tcgtacttag tacttccttt
     7081 tcttcataga tataatcttg catcgtaaac attttctctg ctcctttttt aaaaaatccc
     7141 tatccacgaa ccaaaaattc caaagaaagc tagtccgatc aagatcgtta atggtttcac
     7201 ttctttcttc aatagataaa acactatgcc aaatgctcca agacataaga tatttggaac
     7261 gatatcatca aaaatacttt gaacagtcac agcattttct cctgaaccga tttttaaagg
     7321 aatattgatg gtgatcatcg tggcagtcat tgctccaacg accatcagtc caataataga
     7381 cgcgcctaat gtcaaacttt ccatcatacc gtttgcctgg atctttttca aaaaacccgt
     7441 tcctaagcga tatccccaac ctgttgctag atagcgaaac agaatgtgcg gaatattgaa
     7501 aatcaaaatg aacaagatag ggcctaaaat atttccttgc atggctaaag aggtacccac
     7561 acctgtggca atcaatcgca aagttcccca aaagaaagaa tctcctaacc ctgcaagagg
     7621 tcccatcaaa gatgttttga tactatcgat cgcagtcaca tcaaattcag gatcatcctt
     7681 attttcttcc tccattgcag ccgtgattcc taaaatcagt gtaacgatat gtggggtcgt
     7741 gttaaaaaac tctagatgcc gtttcaaagc tgctgccatt tcttctttcg actgatataa
     7801 ctttttcaaa atcggaatca ttgcataaac ataagccata ttcatctgac gttcataatt
     7861 ccaagaaaac tccatttgaa aagaacgcca aaatacgtga tttagctctt ttttcgttat
     7921 tttgctgttt ttgatctcag aattcttcat cttcttctac ctcccctgca gttgtttgaa
     7981 gtggtagttg acggacattc ataatattga ctaccacaac tgctaaaatc gcaccaaaaa
     8041 tagctactcc ggtcacagga atctctaagt aagaagctat agcaaaacct aagaaaaaat
     8101 atggtgcaac ttgtttgttg atcagcaaac gagcaagcat cgcaaaacca agagctggaa
     8161 tcaaccctgt agctactgac aacccctttt gaacgaagtc aggaatcata tttaatagat
     8221 ttccgatcgt attgcttccc accatataag aaaccattac cacaacacca agcatcaagg
     8281 aaagaccgat accggcaagc aagtgcatat gctcgactcc tttataattt ccttcttctg
     8341 cataattgtc tgctttatgg ttcataattg gaatcaaaag ccctaagtaa atatttttca
     8401 atatcaatgt caacgtagca atcggcaaac ctagtaaaag cgcggtttct gtaccggctc
     8461 cagctgtaat agcaaaagcc gtccctaaaa tcccaccggt cacaacatca ggtggaatcg
     8521 aagcacctac tgagaacgat ccgataaatg ctaactctaa agttgcgccc ataatgatcc
     8581 cagtttttac atcacctaac acgattcctg taaacagccc cgtcacaatc ggacgagaaa
     8641 gcaacgatgt ccctagtgca tattctgatt gtgcgacaaa tgcaacaatt cctaataaga
     8701 ctgcttgtac aatcattgag gttcttcctt tcttacaaaa cttctcttac atccactttt
     8761 ttatctgctg gtacttgacg gatttctact tctatttgtt tgtcaattaa tcgtttcaag
     8821 cgttcctcct cttctggtaa aatatttacc gcttttccga tgctgcgagt accaggtttt
     8881 gccttgatcc ctcctaagtt gatggttttg atttctggat acgcttctgc cagcttatat
     8941 gcatcctcga tcgattccac tacgatgaat aacttatatt tatctgtcac cccgcttttc
     9001 aacgcggcaa tcgagtcatc aattgatttg atcactagtt tgactccttg tggcttggct
     9061 aatttgatcg ttgtctttcg tatctcattc gtaggtacat catcattagc aatcaaaata
     9121 cagtctgcgc ctattgtttg tgtccatgaa aaagctactt gtccgtgtaa taaccgatgg
     9181 tcgactcttg tcaatacaat catttttctt cctcctaaaa ttcgtcttct tctattttct
     9241 gattgagccg ctcgttacaa aactggatca ttttttttga atcagttagc acctgctgga
     9301 tcaaaacagt tgtctctact tcagcgtcca accgagatac tagctcaatc aatagtggca
     9361 aattcaatcc ggcaacgaga tagacatgat cccgattgat atattgtaag aattcgttgt
     9421 tgacacttcc gccaaaaaga tccgtcagaa caatgatttc tttatctttg tacctcataa
     9481 aaagctgcct tactgtttcc gctaaatcaa aattctcatc agtatagcaa tcgaatacag
     9541 taatagaatg atttttccca tagatcagct ctaatgaatc taatatccct gaagcaaatt
     9601 ttccatggga agctaagata atttctctat ccactttttc atctcctctt ttcacttaca
     9661 catactaaaa aagcaagttg catgccaaaa ttagtggcaa caacttgctt atgttcaaaa
     9721 ttcttcttct atcgagagaa actctgtatc ttcactcaaa atatcataga tataggcaat
     9781 ctcagaattg gggatcatga cactataatg ctcttcaatg acactaaacg cagatttaat
     9841 agcagataac ccttcttttt gacactgctt ccatttctct aatccttgat aggtttcgat
     9901 tggcatttgt cggatcaatc gttcaatgag acagctcaca tggacataca acgctaattt
     9961 tttgggattg ctgatgcttc gattcaactg cacctctaat tctctcataa aatgatcgat
    10021 ttctcccatc accttctctg tatctagaat agtcacggaa tccagtactt tttccaatga
    10081 aaaattacga atgatgcgct gattgaatat ttccttctct gaagcattca tagtagtgtc
    10141 caaccattca gctagtacag acatcttttc ccccgagatg aggtcttcta atgaaagata
    10201 gtcgatattg gtgatatcag gatctgtggt gccaatcatt cctacgactt cgtatatgga
    10261 aaataatggg tcactgtttt tcttatcttt taattgctga tattcatatg ggatgatttt
    10321 caactgacaa gccgtaggta agcttttttc tagcagttca cttaagtgga ccgccgtacc
    10381 gattccagtg aagcaagtag taatgagtgc tttgggtttg ttttcctctg gatagagcag
    10441 tcgccattct ggctggatct gtcgaacagt ctcttctgct atctcttcta atgatacatt
    10501 tttttgcagg cattccccta ctgtgattgc taaaggtgtc gtcacattat tcaaaatcaa
    10561 aagcggtacg gataactgtt ttttaaaaaa ctgatggatt tctttcagag atcccatgtc
    10621 tactaatatg atcaatccat ttgaaacatc gctgttttcg caataatcaa ggatttctga
    10681 agcaatctgc tgtggtgtga catctagtgg catatcaaat gattcaaata gatcctcttg
    10741 taataaacga tttactacat ttgccatact gctagcagtt gcataaccgt gagcagcaat
    10801 catcgctttt ggcagtcgtt tttcttttga ccatttcgct ttttttagat agatcgtcag
    10861 taaaatccga tccatacttg tgtcttcaat atctaatttg atcttactta attccagaat
    10921 tcgttggaca tagtgataac tagatggata agactgagaa acttgttgtt ctaattcttt
    10981 gattagttgg ataatatcat gttcttccgg taaccatttg atagtgcttc gctgatagag
    11041 atagtaactc aaggcataga cactgtttcc atcgaatttg atttgatagg cagtttccat
    11101 ttgtcgaaag gtctctcgga tagattgtgt ccaataaacc agcagctcgt gtttttcttg
    11161 tctactggtc tcaaacagca aaaaatcaaa taattgaatc acttcttgct ttaattttcc
    11221 ttcgcaagaa tatagcagac agttattttt cttgaaatca attaggattt gctcaaaact
    11281 tttaacgatt cgctgctgtt cgggctgttg cttctccatt aattgctcta aggtctggtc
    11341 ttgcgtaata tagattcctt gctgtgctgt atttttagta ataggctgcg caagtagttc
    11401 tttttgcaaa tgatggatcg tgacatggat ttcaggctgt tcacgctgtt cagcatatgc
    11461 ttttgctgct gaaactttga cgatattttt cagctctcct atgtttccgg ataatttctg
    11521 acttgttaaa agtgacaaag ctggtccact gactgacaaa gggctcccca tttttctttg
    11581 ttcttcgaga aaaaaagagt aaatcagttc tagccgttca ttacgactac gctgattaag
    11641 tgcaggtagt tcgattcgga taggtattct tcgaatgaaa gttgttaaga aatgactagt
    11701 aatctcttca gtcgtagcaa aaaagagacg tgttttgatc cggcgtgaca aattaggttc
    11761 tcccatacga taaatcacac cttgatcaag ataggtgaac agtttttcct gaccttctgg
    11821 tgttaagcga tgtacctcgt ctaaaaacaa gatcccacca tctgctgctt caaaagcccc
    11881 ttttttattt tcttctgcac cagtgtacgc tcccttaaca tgtccaaaca aattgctagt
    11941 caaaagttca ggattatctg catattgtgc acaattaaaa gtgatgaatg gtgcagaatc
    12001 ttctagcaga tcatgaagca gacaatactg atagaccaaa ttaactaaat aacttttacc
    12061 tgtgccactt tcgcctgtaa tcaaaagtgg taatccgcca tctggatagt aaagagctgt
    12121 tttgatttgt tcgatacttt ctttcaaact ggcgtcgtat ccgatcataa aagagaataa
    12181 gtcttttggt tgcgcgaaaa aaggctgttc ctctttgaga tcatcgatag aaaaataaaa
    12241 attcgtgcgt aacttaaaaa attgtttttc aaaagcttct ttgtggaaat aaatcactgg
    12301 tcgagaattg attttgacta gtagttgctc atcatttaac tggttcaaat aatgactgac
    12361 tgtgtttctt tttaccccaa aatgatcagc taacctttgt gctgtaaaga cctcgttcat
    12421 atgttctaaa tcgaaaaagg ctgtctggtt ctctaagtaa cataaaattt ccttcttcaa
    12481 caacttattc acccctcctc catcatagaa aacggattca ttttgtcaat aaaatcttta
    12541 ataaagaatt gaaaaatata tcttattata gacagcagcc tacttatccc ttataatttt
    12601 tattctaaaa aacgtacagt tccttccagt ttttatactg gaatttcaac atatgaaaga
    12661 ataaggaggc tatgactatt caatgaaatc ggataaatat aaaaacagga aacatacttc
    12721 ctggaaaact tttcgagaaa tcatcggatc ttatctcgct gtagttgtat ttttgccttt
    12781 gctttatatg gctatgacag aacaaactcg ttttattggc gtagatacgc aatatatagc
    12841 cgaaacagga tttttattca tctttatctt gatttttgcg ttctataaat ccttccatac
    12901 aaaataagat tcacagattt caactttcct gtctgttttt agctattaca aatccttata
    12961 tgtggtataa caacaattta ttggcataat gaggaaaaac gagttatgat gctttcagag
    13021 agtaaagagg tactgttgtc accatccttt ttattgatta cgtacaccac tcttagtttt
    13081 ttcccattga ttacgaaaac aaggtagaaa cattgatgtt cctaccttgt tttctttttt
    13141 tattttcaga tcaaaatctc aagtggcttt tgacttgatg tcgggaggtt gtcttcgttc
    13201 catcaaacct gatagaatct gcgccaaacc atagttttct ccactattat ttattcctct
    13261 aagatagtac ccacaataaa cgaataaata tttttctcat tttcatactc cttctttgaa
    13321 aatcaattag atttctctca acccactttt ttacaaa
//
LOCUS       DAZBTF010000074        13086 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000074 DAZBTF010000000
VERSION     DAZBTF010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.074"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..2199)
                     /locus_tag="ACHGNS_002287"
     CDS             complement(79..2199)
                     /locus_tag="ACHGNS_002287"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFN6938210.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2429..3607
                     /locus_tag="ACHGNS_002288"
     CDS             2429..3607
                     /locus_tag="ACHGNS_002288"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFN6938211.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3623..4381
                     /gene="aroD"
                     /locus_tag="ACHGNS_002289"
     CDS             3623..4381
                     /gene="aroD"
                     /locus_tag="ACHGNS_002289"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFN6938212.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4404..4889
                     /gene="ybaK"
                     /locus_tag="ACHGNS_002290"
     CDS             4404..4889
                     /gene="ybaK"
                     /locus_tag="ACHGNS_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFN6938213.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4960..6213)
                     /locus_tag="ACHGNS_002291"
     CDS             complement(4960..6213)
                     /locus_tag="ACHGNS_002291"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFN6938214.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6329..7678
                     /gene="gdhA"
                     /locus_tag="ACHGNS_002292"
     CDS             6329..7678
                     /gene="gdhA"
                     /locus_tag="ACHGNS_002292"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFN6938215.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7790..9136
                     /locus_tag="ACHGNS_002293"
     CDS             7790..9136
                     /locus_tag="ACHGNS_002293"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFN6938216.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9444..9830)
                     /locus_tag="ACHGNS_002294"
     CDS             complement(9444..9830)
                     /locus_tag="ACHGNS_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFN6938217.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(10098..10247)
                     /locus_tag="ACHGNS_002295"
     CDS             complement(10098..10247)
                     /locus_tag="ACHGNS_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938218.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            10709..10921
                     /locus_tag="ACHGNS_002296"
     CDS             10709..10921
                     /locus_tag="ACHGNS_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938219.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(11199..12998)
                     /locus_tag="ACHGNS_002297"
     CDS             complement(11199..12998)
                     /locus_tag="ACHGNS_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFN6938220.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         4291 a         2354 c         2577 g         3864 t
ORIGIN      
        1 ttattctaag atttctcttt accaacatca tttgacaaag agcctaattt tgtcgctgct
       61 ccttttaaat ttattttatc attcggaaga acttgtggtc gttccgtgaa gttcttggta
      121 cgtttgcgtt ttataaagat caacagttga ttgtcccttg tgttcactaa agacactagt
      181 tgacttgtca ccaagagatt tttcagcttc ttttaatttc tctaagccat tcttgtagtc
      241 ataatcactg gtatcaattg ggtctaatcc actattcgta tagaaccgaa gaagatcacc
      301 attattgatt tggtcagaaa cagctaactg tttatcaact ttttctttca gtgaatcaat
      361 ttcattctgt acttcctcag taggttcagt aatagcttca ccagtttcgt tactatagat
      421 cgtaccaccg tagtaagtat attttggtgt aacaaaatca ccatcacgga aagaaacaag
      481 ctgttcatga tctttcgaga atagatcttg tcctaactga atataatttt ttgtatctac
      541 acctagtaag tgaagtaatg taggcaaggc atcaacttgt ccgccgtaag tgtgattgat
      601 tccgccctgt gtttgtccag ggatatggat catataaggg acacgctgca tttgtgcatt
      661 gtcgtagttt gtccaagtag aacttgtttt tccaactaat tcagctagat ttttatttct
      721 tgaattcgag acaccgtaat ggtcaccgta aaggacgatg actgaattat catacaagcc
      781 gctttctttc aaataattga agaattcttg tacggcggta tctaaataat tggctgtcgc
      841 aaagtaaccg ttgatcgttt catcatttgt attggctaac gggaatcctg cttcgtcatt
      901 ggtgaatttt gaataaggat aatggttaga tacagcaatg aattttgaat aaaatggttg
      961 ttgcagacgt tccaagtatt gtacggattg ttggaaaaat ggtttatcat gcaacccgta
     1021 ttggaacgaa ttgtcatcat taacatcata ataagaagaa tcaaagaaat aatcatatcc
     1081 taaattcttg tatgtctcgt ttcgattcca gaatgtacca gcgttcccat ggaataccgc
     1141 actagtgtaa ccttgtgttt gacttaaaat atcaggtgca gcttggaaag tattttttcc
     1201 gcctaattgt gtgaacaagg agccttgatt caagccaaac aatgaatttt ccatcagcgt
     1261 ttccgcatcg cttgtttttc ctgcttttac ttgatggaaa aagttgtcaa aactgaacgt
     1321 gctgttgctg tggtaaagac tattgatgaa aggtgtaact tcaagctgga gaccattctc
     1381 atctgtcaat ttataatcga tcaggaattg ctggaagctt tctaaatgga tataaatcac
     1441 attttttcct ttagcgagcc cgaacaaatc atcatttggt gctgcataat gttcttttac
     1501 ataattttct acttctttca tatcattggg acttgcttct gcccgcactt ggtttgtctg
     1561 gtaagtttga acaccgtcat aaaccatgaa agcattcaag ccaagatatt tgaccaaata
     1621 atcacgagag aatgttcgag tcagcaattc tggacgatca gcttccgcca taaaaagatt
     1681 tcctgagaaa atcataactg ctaatgtaga gatcgcaaaa gccattcttg ctcggatcgg
     1741 gcgagggtcc aacttgattt tttttgcaat cagcagaaca gtcaagataa taaaatcgat
     1801 ccaatagatc acatcatatg ggcgaaacag tcgaatggca ctttctccca gaccactcgc
     1861 tactttccca gcacctaaaa tcgtgttgat cgtaatgaag tccgtgaatt ctcgataata
     1921 agtgacattc gaaaaaacaa gcaatgtcaa aagaaaataa atgaccatca tcgtgatata
     1981 agcaactcta gggcgtctga tataaagagc aattccaagt aataacaatg tagttgcaat
     2041 agggttgata aacaagataa agtattgaat gccactttcg attccgagat ggaaatcaac
     2101 taaataggca aaaatcgttt tcaaccaaag taaaacaact agtaaagaaa aaaatccgag
     2161 cctcttattc aaaaaggcag gtgtttttat acatttcaag ataatcttcc tttctgcact
     2221 tcttaatcac agaagtgtca tgaaaccatg ttacacagct cacttattct catttatttt
     2281 actagtatga caatatagtg tcaattgaaa ccagaagttt atgctgtttc attaaggttt
     2341 tctttaagct tcatgaaaaa atgagcagta agacaagtcc tatgtttttt gttatacttg
     2401 ggaagagaaa gctaaagcag gtgaaggaat gaaagtaaaa gtaaaaaaaa gagcaagcaa
     2461 tcgattgaaa aaaggatatc cactgatcca aaaagaagac cttgctcatc cccaaaaaga
     2521 aaccacgcaa tgggtggaat tagttgattc gcaaaaccaa tttatcgcaa aaggatatct
     2581 aggaaagcag aataagggaa tcggttgggt attgagccag cagaacatcc catttgatca
     2641 atcttttttt gaagaatttt ttgtgaagtc aaaagaaaaa agaactttct ttttttcaga
     2701 tgattctact accgcttttc gtatattcaa tggagaagga gacgggatcg gcggtctgat
     2761 tgttgattat tatgcaggtt atgctgtttt ttcttggtat aacgaaacgt tatatgcaca
     2821 tcgtgaatta ttgattcaag cttttcgtga aatctacccg gaaatcgtag gaggctatga
     2881 gaaaattcga tttgaatcag taaatctgcc tgagagccag ttcatttacg gaaaagaggc
     2941 accggaaccc ttgatcgtaa aagaaaacgg agtaaaattt gccgtttatt taaatgaagg
     3001 attaatgact gggatctttt tagaccaaaa agaagtacgg ggatcattag cagaaggttt
     3061 cgcttctggg aaaacagttt tgaacatgtt tagttatacc ggcgcctttt ctgttgctgc
     3121 cgcaatggga ggggcaatcg aaacaacaag tgtcgatcta gcaaaacgca gtttaaaaaa
     3181 aacacaggaa cagtttgaag tgaatggttt ggatgtcgat cagcaaaaaa tcattgttat
     3241 ggacgtattc gagtatttca aatacgccaa aagaaaacaa tttttatatg acatgattgt
     3301 attggatcca ccaagttttg ccagaaataa gaaaaaagtc tttcgcgtag caaaagatta
     3361 tggggaactg gtcaaggata gtctggatat tttagcagaa gacgggatac ttattgcgtc
     3421 tacaaatgct gcgaatgtct cgatagaaca attccaaaca atgattgaag aggaattcca
     3481 tgccaaaaat gtttcttttg aacaacttgc tttatatcgt ctgccaaaag atttccagac
     3541 gacggatacc tttttagaag gcaattatct gaaagtgttt gtttatcaaa ttaaaaataa
     3601 ccattagaag gagcgatgca gaatggtttg ggtaaaggga ttgaatatag gaagcggtag
     3661 accaaagatt tgtgcgcctc ttatcggggc cgtccgagag gaagtattac gtgaagcagt
     3721 tcttgcacgt gaagcagggg ccgatttagt cgaatggcga gtcgatcatt atcgtgaagt
     3781 tttttctttt gatgaagtag cagaaacatt ggttcttatc catgagatgc ttgatggact
     3841 gccgttatta tttacgtttc gtaccttagc tgaaggcgga gaacaagaca tatcgatccg
     3901 tcagtatcgc gacctttatg aacatgtgat acacacagga ttagtagata tggtcgatat
     3961 cgagttgttc aaagtagaaa gtctaggaaa aagactgata gaggaaatca agtctttaaa
     4021 cattccttta attatcagta gtcatgattt taaagaaaca ccagcagacc ctgtcttgct
     4081 ttaccgattg aatgttatgg aacattttgg agccgatatc ggaaaactgg cagtaactcc
     4141 caataatgaa cgagatgttt tgcggttgat ggagttgact agacgggcaa atgcttttgt
     4201 ctccatgcct attattacga tgtccatggg aaatcttggt aaaatttctc gtctggctgg
     4261 cggaacgacg ggttccgtta tgacatttgg ttccttaagc agcgattcta cttctgctcc
     4321 aggacaatta ccggtcaaag cattgaagca aattattcaa ttattaaatg aaccgttgta
     4381 gaacaagttt gaggtgatat tttttggcaa agaaaaaaat agtaaaaacg aatgcaatcc
     4441 gtatggtcga acaaaaaaag attccctata cagagcacga atatgagtgg gacgaaagcc
     4501 acctaagtgc gtcatcggtc gctgaacagc ttccagaaag ccaatctcat atatttaaaa
     4561 cactggttgc tgtgggcaat gtgacggggc ctcttgttgc tgtgataccg ggagaagccg
     4621 aattgaatct aaaaaaatta gcaaaagttt cagggaataa aaaagtagaa atgctccatt
     4681 taaaagattt ggaagcaacg accggataca tacgtggtgg ctgctcgcct ataggtatga
     4741 aaaaactatt tcctacttat ttagatcaaa ttgcagaaag ctatgaacaa atcattgtct
     4801 cggctggcag aagagggcta cagatggagt tagcacctca agatatctgt gcattaacga
     4861 gcggacaatt tgcagatata aaacaataaa tcatacgtat aaaagaaaga aggaggccgg
     4921 gatgttgttt gtccagtctc cttctttctt ttttatacat tatgctcttt gcccggcatt
     4981 cattcctgct acatgccctg tagcgaaagc cgctgtgata ttaaagccgc cagtataacc
     5041 attgacatcc agtaattctc ccgcaaaata cagaccatca atgattttac tctccaatgt
     5101 tttaggataa atttccttta gagaaacccc tccgccagtt acaaatgatt tctcaatagg
     5161 aaaagttttg ttgattgata gcttaaactc tttgcatagt tgaacaaaat cttgaatttg
     5221 tttttgagag gcttgttgtc ctgtcagatg atccatttca agccgctcaa gaaagaaaac
     5281 taacaatcgc tcaggcaata taccatgcca agcattgacc aagttctttt tggttgcttt
     5341 acttttttct gtcaatatat gaataagctc ttgtttggtt tgtgtaggga aacaatccag
     5401 agaaagtgtc actggttctc ccgttttctc taattcttta ttgacaaaac ttgaacaacg
     5461 cagtgcagcc ggaccagaaa taccgaaatg ggtgaaaagt aaatccatcg tatgttcagt
     5521 caatacacga ccttttttat ttaaaacgcg taaagtgatg tcttgtaaag aaagcccttg
     5581 aagtgttttc tcctgaatat acggttcatc cgatatcaag ggggactctg tcgcataaag
     5641 aggcgtcacc gtgtgaccca ctcgcttcac gatcttgtac ccatctccag tagaaccagt
     5701 agatggataa gtccgtccac cagttgttaa gataatggca ggagcatgga actcttcgaa
     5761 ttccgtgcgt ataccgtaaa ttttatgttc tttgtggatc aatttctcta ctcgagtaga
     5821 gaatagaagc gtcacatcta gttctttcag acgattgagc aatgcttcga taatcgtttt
     5881 tgatttattt gttacaggaa acatccgtcc atgatcctct tcttttaaat gtacgccttg
     5941 tgattcaaag aagttcatga tatcgaaatt attccattgc gcaaacgtac tatataaaaa
     6001 ctttccattt ccaggaatat gtgcaatcaa atcatcaaca ggacgattgt tcgtaacatt
     6061 gcaccgacca ccacctgtca tcagtagttt ttttcctgct tttttgtttt tttcgatcaa
     6121 gagtacgcgt gcgccttgtt cagctgcact gatcgctgcc atcatcccgc ttgttccagc
     6181 gccgactacg atcacatcat acagtttttc cattccctca cctcaaggaa agtgtagcat
     6241 atctgaagaa agaaaacaga aaaaatgttt tgagccaaaa gagaaaatga gctaaaatga
     6301 gggaagataa gcaaaggagc gtggaacgat gacaaacgca acagaatatg ttcaagcaat
     6361 tcaagaaaag ctacatcaaa aggatcaagg gcaagaagaa tttttacaag caatcgatga
     6421 attcatgccc acggttatgt cttttttgga cactcatccc gaatatatcg aaaagaatat
     6481 cttagaactt ttaacggaac cagaaagagt tattcaattt cgagtgcctt ggcaagatga
     6541 tgaaggaaac tggcgagtaa atagagggta ccgtatccaa tacaactcag ctatcggccc
     6601 ttataaaggt ggattgcgct tccatcctag tgtcaatcta agtatcttga aatttttggc
     6661 ttttgaacag attttcaaga atagtttaac cggtctgcca atcggtggag gaaaaggcgg
     6721 aagtgacttc gatcctaaag gaaaatctga taatgagatc atgcgttttt gtcaaagttt
     6781 catgctggaa ttgtcaaaac atatcggccc atcgattgat gttcctgcgg gagatatcgg
     6841 tgtaggagct agagaaatcg gctatttgtt cggcgaatat aaacgattga aaaaatatga
     6901 tactggtgtg ttgacaggta aacctttgga tttttgggga agtaagatac gtacggaagc
     6961 tactggttat ggattagtgt attatgtcaa acatctattg aacgaagaaa aagattcatt
     7021 taatcaaaaa acagtttttg tttccggaag cgggaatgta gccatttatg caatcgaaaa
     7081 agtgcaagag ctaggcggga aagttgttac atgttcagat tctagcgggt acatctatga
     7141 tccagaagga atagatgtga aactattgaa agaagtcaaa gaaatcaata gacaacgttt
     7201 gacggaatac gcaaaccaac gtccttctgc tatttatcat gctggagaat ctgtttggac
     7261 attgaaagaa aaagcggata tcgctttgcc atgtgctact caaaatgaaa tagacgaaag
     7321 cttggccgaa atcttagtag aaaatgggat caaaatcgta gcagaaggcg ctaatatgcc
     7381 atgtacagta aaagctgttt ctgttcttca tgaagcaggc gtgtggtatt gtccaggaaa
     7441 agctgctaat gctggaggag tagcagtatc tgctttagaa atgagtcaga acgcccagcg
     7501 attgacttgg gaaagagagc aagtggatga gcagctggat caaatcatgg aaacgattta
     7561 ccatacgtgt cgccaaacag cagccgagta cggcgacgaa aaaaatctgc ttcttggagc
     7621 aaatgttgct ggatttgaaa aagttgccaa agcaatggca gcagaaggtt tagtttgaaa
     7681 aatgaaactt tcttggaaat attgtcagca ttttgtttat ttgcttgaaa aataataaaa
     7741 aaaaacgtaa agtattacct gtagagtaaa cctataagga ggagaacaaa tggcacacat
     7801 tcattttgac tattcaaaag tagcaccgtt tgtcaacgaa caagagttag ggtacatgca
     7861 aagccaagta acagcagccc acaacgaatt acgggaaggt actggcgcag gaagcgactt
     7921 ccgcggctgg atcgatttac caacgaacta tgacaaagaa gaatttgcac gtatcaaaga
     7981 agcagctaaa aaaatccaat ctgattctga agtattagtt gttatcggta tcggtggttc
     8041 ttatttaggc gctcgtgcag cgatcgactt cttgaatcac acattcttca atttattaga
     8101 tagcaacaca agaaaagcac cacaaatctt ctttgccgga aattcaatca gctcaacata
     8161 tattgctgac ttgatcgaag taatcggtga tcgcgacttt tcagtaaacg ttatttcaaa
     8221 atctggaaca actactgaac cagcgattgc gttccgtgta ttcaaagagc ttcttgttaa
     8281 gaaatatgga caagaagaag caaacaaacg tatctacgct acaacagata aagcaaaagg
     8341 tgcagttaaa gtagaagcag atgcagaagg ttgggaaact ttcgtgattc ctgatgacat
     8401 cggcggacgt ttctctgtat taactgctgt tggtttgtta ccaatcgctg ttagtggtgc
     8461 agatatcgat gcattgatgc aaggtgccgc tgatgcaagc aaagcatact caagcgacaa
     8521 actagaagaa aacgaagctt accaatacgc agcaatgcgc aacattcttt accgtaaagg
     8581 caaagtaact gagttgttga tcaactatga accaggaatg caatacttct cagaatggtg
     8641 gaaacaattg tatggtgaat ctgaaggaaa agaccaaaaa ggcatctatc cttcaagcgc
     8701 aaacttctca actgatttgc actctttagg acaattcatc caagaaggac gtcgcaatat
     8761 ttttgaaaca gttgtgaagg ttgaaaaacc tcgtaaatca atcacaattc ctgaacaagc
     8821 agaagattta gacggtctag gctacttgca aggaaaagaa gtggacttcg taaatacaaa
     8881 agcatttgaa ggaacattgc ttgctcatac agacggtgac gtgcctaact tgctagtcaa
     8941 aattccagaa atggatgctt atacattggg ttatacaatg tacttctttg aaattgctgt
     9001 aggtatttca ggttacttga atggaatcaa cccattcgac caaccaggtg tagaagctta
     9061 caagaaaaac atgttcgcac ttctaggcaa accgggcttt gaagatcttg ctaaagaatt
     9121 gaacgaacgc ctgtaagata agggtttttc gctagattta aataatcagt gatagaaaca
     9181 tgttcgcttt aaaatgaaaa ctggtaccca tttaggtact caaacatact gcatcgttta
     9241 ttcagaataa ggggctgtga caagacgttt gtcacagtcc cttgtttgta tagaaatcga
     9301 ttgattgcct tgctctttag tttgttttag gactttaaga acagtgaggc tgacttcgaa
     9361 ggttaattct gtttttttgt tttctagaat cagtaagcaa aaaagcactc acttcgagtg
     9421 cttttttgcg atatattctt agcttaagat aggcctaatt tttcttttac agatgagggt
     9481 accttatcgc tggtaattac ttttttctct atgatccgct tttcgcttgc tttcaactgc
     9541 aagatcgttc cttcggtgta agcagaacca ttggcagagt caccagaagt aatgacatcg
     9601 aaatctaact gtcttttttc accgttttca gcataagcat ctacgttgta tttaaaacct
     9661 cttccaatta cttccccatt atctgctttg atatcagttt ctgcaggcat tggagctttg
     9721 actaccgcat aataatcttt acctacataa gtagactgat agtagtccca acctttccat
     9781 cctataaaag caatcaatat gataacgatc aaccctaaaa tttttttcat gaaagatctc
     9841 cttttttatt tttattatat taaatattat acagataata acggaatatt atcatcgaaa
     9901 cagctaacaa aattgtcata aaaaacgaga tggttgatat tagaacttgt gtttttgttt
     9961 aggtctagag ataaagagag acaatttcca aatatatgta atgaatacaa aaagacgttt
    10021 gtcgcggatt accgttacaa acgtctttta ttgattttcc gagatctcat cagtcgccat
    10081 ttggaatggt tcactcctta tctagattcg tttaatgctc ggactgcttg ttcgattgta
    10141 ataccaaacg caacgttatt ttcgttttgt gcatcaatcg ccataaaacg gttagaagtt
    10201 gtatcaaaag ctacgcggta ttgcatattt gtttttgtaa acgtcataaa attcatctcc
    10261 tttgtaatgt aatttagttc ttgaaagttt cctttaaact ttcgaactta taccaagttt
    10321 agcatcgtac gttttttgat gcaattaaaa agcatttatc attttcctta tttatttttt
    10381 cttaaatggt ttagctattt tcttttaatc cgataagtag cccaatcatc aaggttacaa
    10441 atactattgc ttgaatataa atcatatgct cacctcttta taaataactt agcaatataa
    10501 agaaaaaaac gcacgaacaa cataggaata taaacaaacg tttttgtcac aacgtctaag
    10561 atcgtgttaa aaaatagtta tctaaaaagc agaaagatac tcaaaataaa aaataaaaaa
    10621 atgaataaaa acgttttcaa atacaaggta atttgttatt ataaaataaa gaaaaaacaa
    10681 aataaaaata aagaaaagag ggaatgaaat ggtaaaagca gaagtaggaa gatcttatat
    10741 atgcaaaccg atcggagtat ctcatgaact aatcggctgt attgaacgta catatatcaa
    10801 cacggctgtc gtgtatgttg aaagttatga ccaacgagac tatcaactta tcaatgaaag
    10861 caagtatcgc atgctcgtaa aattctgtga tattagcgca cctactgaat tggtttctta
    10921 aacagtattt ttgagaattc gcaagaactc ccttataata taaataaaga cagaagggag
    10981 cgagaagata tgccaaaaag aaaaagttca gggaaaatgg gaattattct tgcagtgatc
    11041 tctgcaattc ctgctatttt agctattgtt cgtgcgatga aggaatcaag agaaagttaa
    11101 gcacacatta tgagcgtcaa acaaaaaacg catatacctg cggtggcagg ttatgcgttt
    11161 tttgttgtat aaagactttt ttcattaacg atttttgatt agccgactcc caaaacaaaa
    11221 tttagcagtt tatctgaata agtgatttct tcatctgttt gtttcacact ttcatttagt
    11281 agtcccatct catcggttat gataccatcc acaccataaa acatcatgcg actcatggca
    11341 tctgaatcgt tgattgtcca tacatatact tcttttccgt cttggtgggc agaattaatg
    11401 aagttacggt tgatcgtact atattccatt gtcagaaaat cagcaggcgt cacagggggc
    11461 ccaacaatat tgaaaggtaa gatgtagccc acataaaagt ttggttctgc ttcttttaaa
    11521 tctgaaacgg tttggtacgt caaactttgc aaaatatgat ggttctctaa gataactttg
    11581 cggtatttat ggacaaatcg ttcgacaagg tcatccgaat cttttttaga ggtcttgatc
    11641 tcaataagta atttttggtt tagcttatgt gctttttcca aataagaatc aaaagaacaa
    11701 acaggggctt gcactccgtt ttcagtaact gtaagatttg tcaattcttt caatgtaaga
    11761 tcctgtggtg ttccatttac ccctgttaag tgacgaagat ttgcatcatg catcactacg
    11821 aaatgaccat cttttgtttc ctgaacatcc atctctacat aatcaggatg atagtctctg
    11881 cttgtttttt caagtgcttc taaagtattt tgcgccccat tttgggtact cacacctctg
    11941 tgagagacag tcactggttt tgtctgaaca gctgaagtca gataatccat attgtaaaga
    12001 cagacgccaa taccgaaaaa aactgcaaaa agagtcaaac cagtgttttt cagcgcagag
    12061 aaacgcaagt tgggggcttg tgggataaac cacttaggga tctcaggcaa gaagccttcg
    12121 tcatccatga aatcaacaat gatataaaaa atccctacgg tagacatcac gatattgaat
    12181 aataaaatcc cttgtaaaag cgtcatagca aacacagcgc tgatcaatgc atagtctggg
    12241 aaaaattgtt caaccatact ttgtgccaaa ataacaacga tatatcctaa agaagaaagg
    12301 agtaaaatcg taccgccaat cacaataaat tggcccgtga tggccaaaag acgcgatttg
    12361 gtcaatgacc agctttcccg aatggctgct cgaaaagaac gatctcgtag aatcatttcc
    12421 ggtaaagcaa aaatcagtcg aatcccaata tagccaagga aaatataaac gagtaaaaag
    12481 gaactgacga tgatccaacg attagcaaaa ataaaatcca taatgaaagc ggggattttt
    12541 atttttgaca gtagatcaga attaaaactt aatccgctga tcggtagaat caataaaaaa
    12601 taagcaagga aaaacaaaaa agtaatgggt ctcactttct tcagctgaag tatcgtcaag
    12661 tgtaagagtt gcttcaaaga gattggttct ttctttttaa taaaaaaaac actcatcaaa
    12721 agaaaagtaa attcgaaata aactaacagt aaaatcgtca tcaaaactac gattaacgag
    12781 agtagaaccc caggatgttg agaaaaaatt gaaggaatat tgtcataaga caaatagtca
    12841 attgcccctc ggtgtaaaat aaaacgggtc gtactgctca ataagggtaa acaaataaat
    12901 aaaataaagc catgcatcag caagacatca cggaaataag cttgtgttcc ttttaaaaag
    12961 tcccaagcat tacctaagct acgtttcaaa taattcatcc tatcacctgc tcttcacatt
    13021 gtagcgcaaa tctaatgaaa aaggaaaggg tgcagtgtgt tttgcagacc ctttcccaga
    13081 tttaac
//
LOCUS       DAZBTF010000075        12978 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000075 DAZBTF010000000
VERSION     DAZBTF010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.075"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..512)
                     /locus_tag="ACHGNS_002298"
     CDS             complement(<1..512)
                     /locus_tag="ACHGNS_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HFN6938221.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIG"
     gene            complement(517..702)
                     /locus_tag="ACHGNS_002299"
     CDS             complement(517..702)
                     /locus_tag="ACHGNS_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFN6938222.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(1056..1301)
                     /locus_tag="ACHGNS_002300"
     CDS             complement(1056..1301)
                     /locus_tag="ACHGNS_002300"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6938223.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(1295..1696)
                     /locus_tag="ACHGNS_002301"
     CDS             complement(1295..1696)
                     /locus_tag="ACHGNS_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFN6938224.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(1883..3691)
                     /gene="uidA"
                     /locus_tag="ACHGNS_002302"
     CDS             complement(1883..3691)
                     /gene="uidA"
                     /locus_tag="ACHGNS_002302"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFN6938225.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(3714..5480)
                     /locus_tag="ACHGNS_002303"
     CDS             complement(3714..5480)
                     /locus_tag="ACHGNS_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFN6938226.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(5493..6287)
                     /locus_tag="ACHGNS_002304"
     CDS             complement(5493..6287)
                     /locus_tag="ACHGNS_002304"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN6938227.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(6297..7673)
                     /locus_tag="ACHGNS_002305"
     CDS             complement(6297..7673)
                     /locus_tag="ACHGNS_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN6938228.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(7793..8440)
                     /locus_tag="ACHGNS_002306"
     CDS             complement(7793..8440)
                     /locus_tag="ACHGNS_002306"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFN6938229.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(8524..9951)
                     /gene="uxaC"
                     /locus_tag="ACHGNS_002307"
     CDS             complement(8524..9951)
                     /gene="uxaC"
                     /locus_tag="ACHGNS_002307"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFN6938230.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(10025..11104)
                     /gene="uxuA"
                     /locus_tag="ACHGNS_002308"
     CDS             complement(10025..11104)
                     /gene="uxuA"
                     /locus_tag="ACHGNS_002308"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFN6938231.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(11107..12738)
                     /locus_tag="ACHGNS_002309"
     CDS             complement(11107..12738)
                     /locus_tag="ACHGNS_002309"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFN6938232.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
BASE COUNT         4112 a         2602 c         1657 g         4607 t
ORIGIN      
        1 cctatagtat ctacaggatt ttcttcaata atttcaagta caattgccct tttaaaaact
       61 actctcaact tattgaatat ctgtttaata taatttggtg ataatggttg ctcaatgccc
      121 ttttttacaa catggatttt cgataaaaac tgttgaaatt cctgtatatg aattggtcga
      181 atctttacaa cctgcatttc tccaaaaaaa tccaatgcac gttgtaaaat atgatctgtt
      241 ttcatatatg ttttttctac agttcccaac ttataccaag gaaaaaaata ctctcgataa
      301 aaatcatgaa acgttattcc ttgcagataa attccttgtg aatcagaaaa actttgtttt
      361 aactcttcga gtccccttct agcttctcct tgagttttga agcctcttct ggtcacttga
      421 attctttttc caaagcgatc ttgtcctaaa gttgcagaaa aaaaccaagt gcctcttttt
      481 ctgtctttat aaacattcgc aattttcctc attttactat tcctcccaag aaatatggag
      541 aacttcttct aaaattgaaa taggaattct ttttattttt gaattacgat aaaaaaagta
      601 tcctttctca actaaaagtt ctttacattc cttgaagatt ttattagcat agtactgaga
      661 aaaacctaga ttaatcaaat cttttcgagt agctgttttc attatagcac cttccttact
      721 ttttttcatt tatttctatc agctgctttt gtcaaaagca cataggaatt tcacctctcc
      781 ccaagtttac ggcgagcctt acggaagtat cattatccca actttaaatc atcgcttttt
      841 attgactata aaaattattt tagaatattt atcgctcctt ctttacagca agaaatcatg
      901 gcgcaactaa aatatgctcc ttaaagtgtt aacgtactga tagaatctat ttaattttca
      961 atgaacaata ggttttctac ctaaaagaat ctactaattc cttaactatt agaagattct
     1021 tttaaacaga aaaagtggaa aggggatttt tttaattagt taattttaaa ttttaagatc
     1081 gcttctatta atttaatttc tagctgactt atcatatatt catctacata ggtaacgaat
     1141 tgacctacct cgttaaatcc atttgtcaaa cataacttag ccatataaga atgataatgt
     1201 ttgagtatca tcttgatcga aattggatct ccttctagag cagataagat aatggatagt
     1261 ggaactaata ggtactgccg ctttttagat ttatctacca taggtcatac cccattttca
     1321 attgttcaac tgccttcttt cgttgatacc agataccact tgtgctcata ttgagaattt
     1381 taccgatttc agcatcatta aaacctgcaa aataataaag taggataatc tttcttctta
     1441 tctccgataa ctgatcaatt gctgctgata acttttgatc tgaaattaat atttccattc
     1501 caaataagaa atagacatct tgttcatcaa aatgagtttg ggaatacctg ccttcatttg
     1561 aatctgctaa tggttcctca ttatccttta tgttagcgtt tcgttttcga atagacttag
     1621 cttcgtttcg taatactttt ttacagtagg catcaaattg gtgttgtata gatatgtcat
     1681 tgtaatgttg ttgcatgata atatgctcct ttttccccct ttccactaat acaacaatca
     1741 gaaagcccct ttttttcgaa aaaactagaa aaaaaacata aaaatattta tcattcttta
     1801 aacagaaaat ctttctataa aaaacaaaaa aaaacgatta ggctaggcta atcgtttttt
     1861 gaacattctt aaaaaatcca aattattttt tataactgaa attgggtatt gatgaccatc
     1921 gtttctgtag atagtaagca atcattttag gttttctatc tctagtaaag attcctttct
     1981 tatttccttg cactcttaaa atcccttgac ttgttgcaaa atctgcaaaa ttccacactt
     2041 gttcaccaac aaaattttca catgtatcta ttacttcgtg atttgcttcg taataagcca
     2101 cttgatactc ttctgtaaac attgttggta ctgtatcatg aaatccagct actgtatctg
     2161 ctccatattc tgtaaacatt attggcgtat caggacatct ttccatccat ccatctagct
     2221 cttttttcaa cagatatttt gcatgtgcca gatcccctcc cgaaacatac caaccataat
     2281 aacgatttaa acataaaaca tcgacaatat ctcctacctt acaaacatct ggcgttgatt
     2341 ttagataagt aacgattgtt acaggtcttt tctgcggatc aagttcttta gttaattcaa
     2401 ctaaaggttc aaaatattct ttagcacctt cggaatccga atctggttca tttgcaatag
     2461 accacattac aacacttggg tggttcttgt cgcgttctat caattccttg atcaccgttt
     2521 tatgagaatt ttctgttcgt atctctttcc acgtattatg tttttccaca ttatcatcta
     2581 ataaagtggc catgaaattt aaatgtaacc ctactgcagg tacttcatca ataactacaa
     2641 taccttctcg atctgctaat cgcataattt cttctgagta aggataatgt gatgttctaa
     2701 aagaattagc acctatccat ttcattaaat taaaatcttt aatatttaca gcctcattca
     2761 aaccacgtcc attaacatac gaatcctcat gcttaccaaa tcctttaaaa taaaaaggtt
     2821 tattattaat caaaaatttg ccattggtaa cctgaacact gcgaatacca aattcttctg
     2881 tataggtatc gatcacttta ttatcatcaa ttaaactaat tttcatacta tataaataag
     2941 cgtctagagg ttcccaaaaa attggattct taagctgaat cattcctttc tttccttcag
     3001 cttcagctac aagttgagcc tcttcgtcat atatcgctat taagattttt ccggtccatt
     3061 ctgtatcgat attgaattct acacttgcat ccaaatcagt tattcttgta ttaatagtaa
     3121 tatcacttat atagtctttc ggagttgtat ataacttaac aggtctatga atacctgcat
     3181 aattgaagaa atcaaaatta actaagtttt ccttttttat atccccattt tcctgtactg
     3241 tttccttcat aatccccaca ggtaaagtac tatcgtcaat aatattgttt acagcaactg
     3301 taattcgatt attcccgtct tttacaaacc gattaatttc agactcaaat ggtgtaaacc
     3361 cacctttgtg ttgagtaata aactttccat tcaaaaaaac ttttgcttca tgtgttgcag
     3421 atccaaaacg aagaactaat ctttcttcta ataatctttt aggaagagtc acttcttttt
     3481 cataccacac ccatccaaca tgatttctaa tgttctctgt ttcaaataaa tcattataag
     3541 aagagggaac tgaaatcgga taactctctt tttcatttat tggttctaaa gcaagttctt
     3601 cctgtaagtc atcatttgta tctaatttaa atctccagag accgcttaag tcaattactt
     3661 gcctcgtctc tgtcataatg ggatacaaca tataaaccac tcctatctaa atattaaata
     3721 aatgtatcct ttaaaccaca ataagcatct attggactta ctcccttaaa ttcagaaaga
     3781 cccattagtt tgtccaccaa tatttctaaa gtttcttgtt ttccatcata agtattaata
     3841 taagtcttta cttgaggtac atcagccaaa tgaaacggac actgtacaga tacaaaaatt
     3901 gttggaattt catgaatgta aaacggtata tccggagttc ctttacttgc tggccagaca
     3961 attcgttgaa ctgtgcctgg attaacatta gctatattaa tgatcaagtc atatttttta
     4021 gttaaatttt taattggtct cttagcagca tatattccag ctatattttt aattttttct
     4081 tcttgcgata atcctttcaa ttcttcttca ggtgaaatgt atttttcaac ttcaaatcct
     4141 tttgctgaca aaatctgttt caataaatca atggcttgag gtctatttcc agcaactact
     4201 tcaccaaacc caccttttac cccatgtaca tctactagta aaatcctttt atatttttct
     4261 ggtacaatag gccaaatatc ttgtttatct ttaactaatg taattgcttg gtcagccacc
     4321 tctttaaaaa ttgatttatt ttcaattaaa ccaatttttt ccagagcttc ttgcttaggt
     4381 aataaaattt gttcttttgc ttttttgtgt aatcccaatt tagcttttaa ccctaaaatt
     4441 ctttttaagg catctaataa ccgatcatca gtgataattc ctttacgata gccttccatc
     4501 atataattaa agtcttcatc tggatcatta aaaaatagaa atagatcaca tccagctgca
     4561 atagaagtgg gcaataattc tgatcgtttc attgccccag tcattcctaa catatgacta
     4621 gcatcagtaa ctactaatcc attaaatccg agttttcctc taagtaagtc agtgattaat
     4681 tcttttgata aagttgcagg aagtaaatct tcatctttca gatcaggatt aaaatgtttt
     4741 gaataagctg gcatatggat atgacctgcc ataattgaag gtaatccagc ttctattaag
     4801 cctgagtata cttttccaaa agaattatcc cattcttccg tacctaaact attaatacta
     4861 aaagatagat gctgatctcg ttcgtcaata ccgtcaccag ggaaatgttt agctgcaggc
     4921 aaaattccac tctcctgaat acctttcata taagctaaac taagttctaa aactttatca
     4981 gggttttgtc caaaagatcg actagaaatg ataggatttc tccaatttct attaatatct
     5041 acaataggag caaaactcca gttacatcca atagccgatg cttcaatccc agaaactctc
     5101 cctaattcat aagcatattt cgcatcattt gttgcaccaa tttttacttc tacaccaact
     5161 tcagtaccat ctgtacaagc tccgttacca cctgcttccg tattagcagc aattaataga
     5221 ggaattttac tttctgtttg taagatatag ttttgatcat atacctcatc agcagttcct
     5281 ggattatatc gtacagctcc aatatggtac tgattgacca tattactcaa gtaattttta
     5341 tcacgactag atcccatatt aacaaacaat tgacctattt tttcttccaa agtcatagaa
     5401 tcaatcgtct cttcaatcca agaaatagca tcttcatcta aattatatgg tttttttcgt
     5461 aaatctactt ttatagtcat tttctttctc ctctacttat tagtaagatt tttccaaaaa
     5521 gtatctatat atttttgttc aatatctaca taatctcccg ataaacattt aaataaaaca
     5581 tcagttctta agttttccca tgattctttt tctttattga ccaaaatagg ataaaagaaa
     5641 actttatttt tatttgcagc ttctagatca ccaggagaat caccaatcat taatatctta
     5701 tccaaatcac aaccattttc aattacactt cgaataatat cttctttttt tcctttatct
     5761 tgacaacaca gttcatcaac taatgataat aattcatgtc tttcccattc ttcttgaaca
     5821 gcttccttat tagctgagct tacaataatt atcttcccat attttttcag agcttttata
     5881 gtatctctag cacttaaaaa gggtttatct aatccttttg cttgactaat ctgttgattg
     5941 acttcttccg accattttaa agcttttaat aaatcctctg actgagaata ttctatttcc
     6001 ctcttcaaag atggattaga caattctttt gttagagata cccagtgctt taatcgatta
     6061 atcgatgtta catcattttt ttctaatgtt ttaactaaac ctaaaaatct gtttattcca
     6121 cgagtctctg aatataaatt aatattattc caatcctgta ggaatttttc tctatgatga
     6181 acatcaaaaa tctctgcagc aattggtccg aaaaagagat tgtgtttata agtcatcgta
     6241 tccataacac agccatctga atcaatacaa aatatccaat tattcatact tctctcctat
     6301 ttatgttttt gttttaaaag ctcgcaattt tctaagactt tatctttaga taatggataa
     6361 aggaaaaata aaattaatcc tacaatcaga aaacaaattg atggaattaa tgttgaagcg
     6421 ttatataact tttctaaaac atctcccgtt tgtacatttg ctaaagagtc atagcctata
     6481 atagaaagag tccaacctcc gacgcctcca gctagagctt gacctatttt tctagcaaaa
     6541 gaatataccg catatacggt cccatcttct ctttgatcag actttacttc ttgatcatca
     6601 ataacatcag taatatttgc ccaaataaca gtattaaaca cacccaaacc catatagcca
     6661 accgaactaa gaattaagaa tacatacata ttactagctt ttatcaaaaa taagatcaaa
     6721 tatactattg ctgaaaagaa cattcctact gatgccagtt ctttttttcc atatttttta
     6781 cttaaaaaca aagttaaagg cgaaacaact attaaaacca aaataggatt gataaaattc
     6841 attaaaacta ttccctgtgc atcattatag taatcaggaa atacataatt attcatactc
     6901 ataattaata attgagcaag gagtaagaaa atagccgcac caattataga aagtaatgcc
     6961 ctatttttaa ctaacccttt taaagatttt aacaatgaaa ctttgtattg agattggtca
     7021 attcttactc gttcagtagt tagtttataa catattaaat aacaaataag tgcacatatc
     7081 gaaaatattc ccgcaacaat tgtaaaagtt gatccaccac gaactatttg agcgcctgaa
     7141 tcatctcttg tataaataaa taatggtgtt aatacaccaa tgaccaaact tgctaaagta
     7201 gctccaacat ttcggaatgt cgatagcatt gttctttctt ctgtaataga agtaatagct
     7261 gatgccattg atccataagg aatatttata gatgtgtaaa aaatactccc ccataatata
     7321 tacgtaacat acatgtatac taccttaacg ctcatagaag ctgaaatcat agaagtttga
     7381 tacattaaaa aacttgctaa agcgactgga ccactcattc ttaaaatcca cggtttaaac
     7441 ttaccctgct tagttgcttt cgatctatca acaatacgcc ccatagttac atcagtaact
     7501 gcatcgataa atcttgctac taaaaataat gttccaacgg cagctccgct tatccccaat
     7561 acttttgtat aaaagatcat taaaaaagaa ctagcaaaaa tgaaagtaaa atcgtttcca
     7621 aaatctccaa acatatatcc tattttgtct cttataccaa attttctttc catacaattc
     7681 tctcccttct gttgttaagc gttttcattc tgatgaaagt acgtactata ctctgaagcg
     7741 taaagagtca gtatttattt tttacatttc agagtattat ttgttttttc tcctaataat
     7801 tctttatctc ataaaactta tctatatatt tactagctac tcgacttact tcttcaaaat
     7861 cacctttttc aacagaagcc aataaattcc cacctacacc aactgcaaca actcctactt
     7921 caaaccaatc ttctaagttc tctatactta ctccgccagt aggcattata tttacgtgtg
     7981 gtaatggagc ttgataggct tttacaatgg atggaccaaa agcagaacct gggaatagtt
     8041 taatgatatc tacgccagca gtaagtgcct cctttatctc agtaattgtc atacaacctg
     8101 gtaaataagg aatttgatat aaattacaaa tctcagctgt ttgtcgatca aaagatggac
     8161 taacgatata ttctgcccca gccataattg cgatacgagc agtaaacgca tctaaaaccg
     8221 taccggctcc gattattaca tctttttgat cagcatattt tttagcaatt tctttgatta
     8281 tttcttcagc attaggaact gtaaaagtta gttcaatccc tttaattcca cccgcaataa
     8341 ttgaatttat tgctttaatg gcctcatcct tatttttccc acgtacaacc gcaatgactc
     8401 cagccgcttc taacttactt aaaatttcta cttttttcat atctatctcc ttcctttatg
     8461 gatacatact ttgtctagct gtactaactt acaaagaata aatttaagct tttatttcaa
     8521 gtattatttt ctacttccga tttcaaaaaa attaaaataa ttatacgcat tacgataaca
     8581 aatattctcc actatctgac taagatacat ttcatcatct ggaattcggc ctgctttaac
     8641 ccactcgctt agtaattgac ataatactcg acgaaaatac tcatggcgag gatagcttag
     8701 aaaacttcga gaatcagtca acatacctgt aaaattcgac aacaaacttt gttgtgccat
     8761 cgtagtcaac tgttgacgca ttccttcagc tgtatcattg aaccaccaag cacatcccaa
     8821 ttggattttt tgaatcatac ccccttggaa gctttgcatt gctgttgcta gtggaagcca
     8881 atcagttgga tttaatgaat ataaaattgt ttttggtaag cacccactct tttcagcggt
     8941 atcgaacaat gctacaacat tttgtgtcaa actagcttga tcccccatcg aatcaccacc
     9001 gacatctact ccaatttgtt cctttaatat acttgaatta tttcttaaag catttaaatg
     9061 catttgcatc gtccaaccat atcttgtata ctcagacatc aaatgtaatt gaatacctgt
     9121 aacaaattgt gcaatttctg ataaatggaa atccgttttt cctgctaaag cttttattaa
     9181 cagattgtcg agtatttttt catctgctat ttcataaaaa taagtattca aaccgtgatc
     9241 tgctaatcga cagccaattt gatgaaaatg atccactcgt tggctcaatg cttgcttcaa
     9301 cccattaaag tctttgattg gtatagccgc tgtttgacta agttttaaaa tatagttcga
     9361 aaagcctgat gatttgatac tccagacctt atctggacgg aaagtaggta ataccttgaa
     9421 agaattatcc tgtttagaaa gctcagtatg ataccacaag tcatctgtag gatcatccgt
     9481 tgtacagatg gctttcacat ccatcatctc caacaattct ttcgggcgat aggaatccgt
     9541 ttgaattttt tcgttagcac gtcgccaaat ttctggtgca ttcttctcag aaattgtcaa
     9601 atcaatatca aagtaacgtt gtaattccaa atgagaccat tcaaaaatag gattacctgg
     9661 cgccttctcc actgttttta caaaagctaa aaatttttca taatcatcac ctggaccact
     9721 gatcaactct tcttctatac cattagctcg catcaatcgc catttataat gatctccagc
     9781 tcctttatca tttaaccata cctgagtcaa attcttgtat tgcttatttt ctagcaaata
     9841 tgctgggtct aaatgacaat gatagtcgat aattggttgg tgttttgcaa atttatgaaa
     9901 aagtctttta gctatttcat tctctaataa aaagttctta tctaaaaaca tatttaaaca
     9961 ctcctttcct tccgttagtt tttagaagag aaaatttttc ctctaaataa actcccttat
    10021 ataattattt actcgccccc ttttcaagcg cttcccaaat accatttaaa taaactgcac
    10081 ctagagctcg gtcatataat ccataaccag gtctaccggt ttctccccaa atcatccgac
    10141 catgatctgg acgaatatag ccattaaatc cattatcgta cattgttttt aatatcttat
    10201 acatatctag agaaccatat tctgatttat gcggcgcttc gtaaaaatcg agtccattgt
    10261 cacttaaata tttgatatta cgaacatgtg caaaaggaat ccgatcccgt tgacaaaatt
    10321 cagatagaat ttcatagaca tcattatctg gtcgttctgc aatacttcca gtacataatg
    10381 taataccatt agaaggagaa tccactacat tacacaacca atctaaatcg tcacgatttt
    10441 taacaatacg aggtaatccg aaaatggaat atggtggatc atctgggtga actgccattt
    10501 taatcccaac ttcttcacat gtcgggataa ttgctttgag aaaatatgca aagttctctc
    10561 gtaatttctc ttcatcaact tctttatagg cttctaataa ttgcttaatt tgtgataacc
    10621 gatgaggttc ccaacccggc aattcaaaat tattagtaga attttctact tctctaataa
    10681 tttcctccgg atcagtaggg atattttttg aaatataggc taacgtagaa gacccatcat
    10741 ctaaacgata atccaaatcc gattttaccc aatcaaatac tggcataaaa ttataacaga
    10801 taacctcaat cccaaattct gccaaattgc gaatcgtctg tttataattc tcaatgtatt
    10861 gatctcgtgt tggtaaacca attttgatat catcatgaac attaacactc tcaataactt
    10921 tcaaggtaag tccattggct tcgacttcat cttttaaaga tttaattcgt tctctcggcc
    10981 aaacctctcc ggctggtata tccattaatg ttccaacaat tcctttcatt gctgggatct
    11041 gacgaatttc ttgtagtgag attttttctt catttgcccc ataccatcta aaagtcattt
    11101 ccatagttac tttcctccaa cttctgttat atatttattg attgttttac gaactgcttg
    11161 aggacctact agcatttcgc aaaagatttt ttcagttttc tttcctaccc caacttgata
    11221 taaatctaaa ccaaagattt ctttattttc taaaatttcc tgtaccgcac aatgaatctt
    11281 tgaaggatct tgttcaccta atttcaatac tgataattta tcctgcaact ctaccaataa
    11341 cggatcagga cttggcacaa aattttcccc ttgatcatcg actgccagta aataacgtaa
    11401 ccaagcagca atagcgatag gaataaatgt tagattttct gcatttccgt ctttttcgac
    11461 atacttttta attgtttcac catagcgaat cgctactttt tgagaagtat ctgttgcaat
    11521 tcgttgtggt gtatctggaa tatagggatt aggcaaacgt ttattaatta cttcatcaat
    11581 aaattgtttt ggatcaataa tgcctggatt ttcaactact ggtaaacctt ctaaataacc
    11641 tattcctttg attaaaccaa ccaaatcatc atcctgcatc tctgctgaaa ttgattgata
    11701 tcctagcaga caacctgtta ctgccaaagc agtatgaagt ggattcaagc ttgtcgtaac
    11761 cttcatgatg tctgctttat caaccgtctc tctatctgtc ataatcacac cagccttttc
    11821 caatttaggc cggccatttg gaaaactatc ttcaataact aaataatgaa ctacctcagt
    11881 attagtgaaa gcagcaatat tggtatgttt ttttgtgtga atgatatcta aatcctcgag
    11941 ccccatcatc aatagatctt cctttacatt ctctgaagga tttggcgtaa ttcgatcaat
    12001 catactccaa ggaaaagtca ctttttcttt atcctttaaa taagcaataa atttttcaga
    12061 aacatatcct tctttttgcc aagcttctgc aatcgtaaaa atactatctt gaaaacgttg
    12121 accattctgt gagaaattat cagtagaaac catcgcaatc ggcaactctc cagcattaaa
    12181 tctgctatat aacaaggatg ttacaatact catcgtatgt tgaggattgc taggtccaga
    12241 tttgatatca gctttgacta ccgaaaagaa ttgcccttta gaatctttta aactgtaacc
    12301 tttttcagta atagtaaacg ttgtaaactg taatttagga tcttcaaaat atttaaccac
    12361 tctttgatag tccgctatat tcttcggatt gcaatataaa ctatcagccg ttactgccaa
    12421 aagtcttttt ttgagttcac catctccact cataacaatt tccaaaatgt cattgttata
    12481 gtcatgataa gctttttcca ctacttcttc atcaaatgtt tcacacacaa ctactcctgt
    12541 ttctaattca ttttgatctg ctaagcattg agcaatttct gcatgaaatc ctcgataaag
    12601 atttcctcca ccaaaatgaa gccatactgg atgttgctgg ccttcttgct taacttcttt
    12661 gacatcaaaa cacggcagta gaatctgcat ttcattgaat tcttcgatag aattttgcca
    12721 atttttaatc gaaaccataa ttaacctcct caaactgata tgttagtttt gatacatttt
    12781 atattttaaa acccattgta accgttgtca atataaaaag ggaataaact tgcttttttc
    12841 taccatatca gaatgattgt attttttatt cagaaaaata aaatactcct tctttttgga
    12901 ctgattttat cgaaacttat tttttctttt tttcatgtat tcttttattt ttataactag
    12961 tgtattactt caaaattt
//
LOCUS       DAZBTF010000076        12866 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000076 DAZBTF010000000
VERSION     DAZBTF010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.076"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            109..1395
                     /gene="efmA"
                     /locus_tag="ACHGNS_002310"
     CDS             109..1395
                     /gene="efmA"
                     /locus_tag="ACHGNS_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFN6938233.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(1533..2132)
                     /locus_tag="ACHGNS_002311"
     CDS             complement(1533..2132)
                     /locus_tag="ACHGNS_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFN6938234.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            2536..3657
                     /gene="mnmA"
                     /locus_tag="ACHGNS_002312"
     CDS             2536..3657
                     /gene="mnmA"
                     /locus_tag="ACHGNS_002312"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFN6938235.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            3903..5324
                     /gene="arcD"
                     /locus_tag="ACHGNS_002313"
     CDS             3903..5324
                     /gene="arcD"
                     /locus_tag="ACHGNS_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFN6938236.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            5338..6456
                     /locus_tag="ACHGNS_002314"
     CDS             5338..6456
                     /locus_tag="ACHGNS_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFN6938237.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(6516..7808)
                     /locus_tag="ACHGNS_002315"
     CDS             complement(6516..7808)
                     /locus_tag="ACHGNS_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFN6938238.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            7958..8518
                     /locus_tag="ACHGNS_002316"
     CDS             7958..8518
                     /locus_tag="ACHGNS_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN6938239.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            8647..9024
                     /locus_tag="ACHGNS_002317"
     CDS             8647..9024
                     /locus_tag="ACHGNS_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFN6938240.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            8957..11029
                     /locus_tag="ACHGNS_002318"
     CDS             8957..11029
                     /locus_tag="ACHGNS_002318"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFN6938241.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(11240..12187)
                     /locus_tag="ACHGNS_002319"
     CDS             complement(11240..12187)
                     /locus_tag="ACHGNS_002319"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN6938242.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            12433..>12866
                     /locus_tag="ACHGNS_002320"
     CDS             12433..>12866
                     /locus_tag="ACHGNS_002320"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFN6938243.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQ"
BASE COUNT         4146 a         2338 c         2745 g         3637 t
ORIGIN      
        1 attgaaacga ttgttattta gggaaaaagg tattttttga aaaaagaatc ccgtataatg
       61 attgttgtgc aaacgctata caaaatgaaa aagaaaagag ggatgtttat ggaaaatgaa
      121 cagtcggtcg ttttaacgaa ttggaagaga aattatttgt tctttttatc gggacagttt
      181 ttgtcaggaa tcactagtat ggtcgtccaa tatgcgatca tctggtattt gacaagagaa
      241 accggttcgg cgacaatttt gagttttgct actttactag gaatgattcc catggtttta
      301 ctaagcccat ttgtaggacc gctagtggat cgctgggata aaaaagctct tttgattgtc
      361 acggatatta ttgtagcgat tttcgcgctt attttagcag tagtcggaac gatttcagaa
      421 tccttcccga tttggctagt ttttgtgtct ttgtttatgc gttcggtcgc acaaacattc
      481 caaatgccaa cgatccagtc gattatgccg acaatcgttc catcttctca cataacaagg
      541 acgaatggac agttaggaat ggtccaatca gcgaatttca tcattgcgcc agctctaggt
      601 gcggctttgt tttcagtcgt tcctgtcaat taccttattt tattagatgt attgggggca
      661 gtttttggtg tcggcttatt gatttttgtg aagatcccaa aagtttctcc tgaaatattg
      721 gaagttccgc ttactatttt taaagatgca aaatttgggc tgcagcaatt gatggataat
      781 aaagggttat ggtacataac gatcaatggt gcatttgtga tgctgttgtt catgccagca
      841 atcagtctgt atccattgat gacgctagat tactttggtg gttccgttgg acaggcggga
      901 gcagtggaag tagtctacgc agtcggaatg ctcttaggcg gtgcactgat cagttttata
      961 ggaacatgga aagaccgaat gaaacctatt attatagcct acatcattat gggcctgacg
     1021 atcggtgcaa gcggattggt tccaaacgat agtcaagggt tcttgtactt tcttatcttg
     1081 aatgcaggag caggttgtgc aacgccatat ttcaatacac tgctgatggc aatgatccag
     1141 cagagttatg aatcgaatgt tttaggtcgt gtcttaggga acttcaactc tttgatgaac
     1201 ctggcaggtc caattggatt attatttgca ggaccattag ctgatcgttt aggggtagaa
     1261 aaaatgttct tgttttccgg aatcgggatt ttattatgcg gaatcgtttt atttttaacg
     1321 tcagctgccc gaaattatga taaagaatta cagaaaaaac tggtaaaaga acaccatgag
     1381 caaaaagacg aatagtatgg aatggagttg tgacagaagt cttgttacaa ctccatttct
     1441 gatcctaatg ttcctgtggc aataagccta ctcaaatgaa aaatatgagt aattatcttt
     1501 atttgtttaa tacttgatat agggcagttt tttcacgacc tatcgtactt cctctttgct
     1561 tcatagattt ctttgatttg ttgctcagat aacggagtgc caaatggtag tttttccttt
     1621 aagagttctc tttccggtgc gtctacgcct acattgacat cgttctcgat aggtacgctg
     1681 taatgatgga tatgtccatg aagattgcgg atagtgtcgt taggcccgag caacatcgga
     1741 taatgagtca agtaatattg ttgatggtcg aatttaacga tggcacctac atcataaaaa
     1801 taaaatttgg gacttccgtc agtaagccta ggatcatttt tttctaaata acggaaaaaa
     1861 gcgcgactgt catggttccc tttgatcaag tggattgttc cttgcagttg agtgaccagt
     1921 tccaaaattt ctgcatttgc tttttcatat tgcggatgca tagcaaggtc gcctagatga
     1981 taaacatggt cggtttcttt tattactgca ttccagctag tgatcaactg ttggttcatt
     2041 tcatccacat ttgcaaagag ccgcggagca aagtcgtttt ttcctaataa gcgttcatgg
     2101 aaaaagtgca tatcactgat aaagtaattc atgtatcttt tgcctccctt ttttcttaga
     2161 gtaaacaaaa tcacttatct gagcaactca taacatctaa aaagaaaagg ttgtaaatga
     2221 gacgtgcagc tttaagcgat aagaagcaat tttgaaaaat agtttttcac aatttataaa
     2281 attattcctt attgccgaaa ggctggccct ttttatgagt atgccaattt tttatatcga
     2341 atgctcaaaa gatgagtgat tttttggttg actatcatgt ttgtgtactg aataggtcca
     2401 gtagaacagt atttttacga cttgataatt aagggatgaa attttcagaa atatgacgta
     2461 taatgtaaaa tactgaaaag ttgcgacctt caactcgtag atgtttcaga atctaattga
     2521 aatgatggtg atatcatgac agacaacagc aaaacacgcg ttgtcgtagg gatgagcggc
     2581 ggtgtcgact catctgtaac agcacttttg ttaaaggaac aaggatacga cgtgatcgga
     2641 atattcatga aaaattggga tgacacggac gaaaacggtg tatgcacggc cacggaagat
     2701 tataaggatg tagcgaaagt agctgctcaa atcgggattc cttattactc tgtgaatttt
     2761 gaaaaagaat attgggatcg tgtatttgaa tatttcctag ccgaataccg cgcaggacgt
     2821 acacctaacc cagacgtgat gtgcaacaaa gaaatcaaat tcaaagcatt cttggattat
     2881 gcaatggatc ttggcgccga ttatgtagct actggacatt atgcgcaagt cactcgcgat
     2941 gaaaacggcg tggtccacat gcttcgtgga gtagacaata ataaagatca aacttacttc
     3001 cttagccaat tatcacaaga acagctttca aaaacgatgt tccctctagg cggcatggaa
     3061 aaatcagaag tacgggcaat tgctgaacgc gcaggccttg cgactgctaa gaaaaaagat
     3121 tcaacgggaa tctgctttat tggtgaaaag aacttcaaac aatttttgag caactatctg
     3181 ccagcaaaaa aaggaaatat ggtcactctt gatggtgaag tcaaaggaca acatgctggc
     3241 ttgatgtatt atacgattgg tcaacgtcaa ggactaggga tcggtggcgg aggagattcg
     3301 caagaaccct ggtttgttgt aggaaaagat ctagcgacaa acacattata tgtggggcaa
     3361 gggttccatc atcctgcatt gtatgccaca agcttggatg caagtgaaat ccactttacg
     3421 acgaatgaac caatgccaaa agaattcaag tgtacagcga aattccgtta tcgtcagcaa
     3481 gacgttcctg tgacagttcg cttattggat gataatcgtg cagaagtcgt attcgacgaa
     3541 cctgtacgtg caatcactcc tggacaagca gttgtatttt atgatggaat ggaatgtctg
     3601 ggtggtggtt tgatcgacca cgcatatcaa gaaacaaaag ttttacaata cgtttaagcg
     3661 atattaaatg aatcaaaaca gctatggcta acaaaagaaa caccttttct tttggatagc
     3721 atagctgttt ttttgcaata aaattaattt ttattcattt tatacttgaa atacttgtgt
     3781 aaaaagttca taatatccaa taatatgaat aaagttgctt tttgttaagc aagagcagtt
     3841 gcaaaataga agggaatctt ttgcaagtga tttgttcaat tttataagtg agggtgatgg
     3901 aaatggaaaa gaaatcgacg ggtattagca aatggggact ggttgctctg gttgtcagtt
     3961 cgtcaatcgg aagcggtgtg tttgggatca caagtgatct ggctagttct gcagctccag
     4021 ggccagcgat cctttcatgg ataatcgttg ggattggtat tttagctttg gtattatcgc
     4081 tgaatcattt aggagaaaag cgtccagatc tagatggtgg tattttcggt tacgcggaag
     4141 cttcatttgg taaattaggt ggcttcatca gtggttgggg ttactggttg tctgcgtggc
     4201 tgggaaatgt tgcttttgca acgatgctga tgagcgccat gggagaattt ttcccgatat
     4261 tcaaaggtgg acaaaatgtt ccttctattt tatttgcaag tatttttatt tggggtctga
     4321 ctttattagt aaacaatgga gttgaaggag cgagcgtcat caatacaatc gtgacgatct
     4381 gtaaattagt gccattattc ctatttttaa tatttgtatt tatcgcattt aaagtgaata
     4441 tattcactgc tgatttttgg ggtaacgtat ctgataatct agtgaatgga acagggcagc
     4501 aaggaacgat ctggttgcag attaaaggtt gtttgatggt tatgatgtgg gtctttgtag
     4561 ggatcgaagg cgcaagtgtt ttagccaacc gcgcgaagaa acgttcagaa gcccagcaag
     4621 catcgatcat tggtctgctt tgtttattgt ttatctatat ccttgcttcg atcttgccat
     4681 acggcgtctt atctcaagaa gagttagcaa ctatttcaca acctgctatg gcaaatattt
     4741 taaaaggaat cgtgggaaat tggggagcag cgctgatcaa tatcggcttg attatttcga
     4801 tcattggttc ttggttgtct tggacgatgc tcccagcaga aacaacgatg ttgatggctc
     4861 gagacggtct gttgccgaaa ggttggggaa aaacgaataa aaaaaatgcc ccaacttatt
     4921 cgttagttgt tactgcaatc ttgactaatc tattcttatt gactttctta gttactgatt
     4981 atgcctatca atttgcctat tctttatgta cagcggcaat tttgatttgt tatctacttg
     5041 tcgggatcta tcaagtccag ttttctttaa aagaaggaat caaaaaacaa gtagtgatcg
     5101 gtgggattgc tgtagttttt gaattgatgg gcatcatttt agcaggattt tcttatgtcc
     5161 ttttatgtag tattgcctat cttcctggtt tttgcttcta ctggatcgct tgtcgtgaaa
     5221 ccaaacatca aattacggga aaagaaaaag taatgattgg gctgatttgt gtggccgcac
     5281 tgctttcaat cgggttgctt gctgtaggct ggatcaagat ataaggggga aagaaacatg
     5341 aaaatcgcaa atttcggtgt agaagaatgg ctaaataaat gggaaaagaa agcggtatat
     5401 gatattgcac aaagctcgat cgaagcattg acattagacg aactcgttgg cttagatggg
     5461 actagcgtaa gtaaattttt cgaaaaacgc aaaaacgaac cattggacta tggctggatc
     5521 gaaggatcag atgttttcaa acaagaagtc gcttctcttt atcaaaccgt cgatccggaa
     5581 aacatcttac aaacgaatgg agcgactggt gctaatcttt tagctctata cgcgttagtg
     5641 gaagcaggag accatgttgt ttcgatgctt cctacttatc agcaattata tgacatcccc
     5701 aaatctttag gtgccgctgt tgactttgtt catttgaagg aagaagaaga ttggcagttt
     5761 gatttagaac agctagaagc cttagtcaaa cctgaaacaa aaatcatctg tttgaatagt
     5821 gcaaacaatc ctacaggcac attgctagat cggaaaactt tagaaaaaat cgcagaaatt
     5881 gctcgtacag tagatgcata tgtattgata gatgaagtat atgcgccttt aacggataaa
     5941 ggtgaattct tgtcgatcgt agatgtctat gaaaaaggta tagcaaccaa ttccctatcc
     6001 aaaacctact ccattccagg tattcgtatt ggctggacag ctactggacc tgagttggca
     6061 gaagtttttc gaaagtatcg agactacacg atgatttgcg gcggagtcct gtcagatgat
     6121 cttgctgtac atgcattgaa gaataaagaa aaaatattgg cgcgaaacaa aaagatcatt
     6181 acagagaacc ttgtgatttt aaaacaatgg gtagccaatg aaccgaaagt tgaacttgtc
     6241 gctcccaatt acgtctctac ttctttcatt aaattagcca tcgaagaaga tgaccagaca
     6301 ttttgtatca atttattaga agaaacagga gtactattag taccaggaag tgcctttgat
     6361 ttaccgaagc atgcccgact aggttactgt tgtaaaaaag aaaccttgga aaagggatta
     6421 ttcctcttat ctgagttctt gaaaaaacag gcctaaacaa aaacactgca aggacagtct
     6481 gtgacaactg ttcaagcagt gtttttcatt tacgcttatt gtctatgaga aggagagtgc
     6541 cctgaagcta tggagactcc tgaagctttt tcttctgcag tttcttgctg atggatctct
     6601 tgtgtattgt gtcgtacaag caagaagttg accaaaacaa gaagagaagt cgaaagaaag
     6661 acgccgcgat aaccaaatgc actggaaaca ctggagccga tcataggccc tactacgtta
     6721 ccagttgctt ggaaagattg gttataactg aagatccgtc cagcagcttg atgaggcgaa
     6781 tattttgtga tcaatgcttg gacagcaggg agcagacaag catcagagat accaatcaaa
     6841 aaacgtaagg cagctaattg ccaaacattc gtgacgaatg ccattgggat ataaaccaga
     6901 atcgcaaaac ccaaaccaat cgcaagtatt ctttcactcc caatcttgtc acctaagcga
     6961 ccaaatcttg gagcggctat caaagtggca atcccaggta tcgaagcaat gattccgctg
     7021 atcaacgtaa cattcccatg tccatgcagc aattggcgaa tgtacagact gatgattgga
     7081 ctaatggaat tgttggatgc ttggatgatc atcgttgtga cgaacatacc gatgacgacg
     7141 tgtggatatt tcaagtcttt gaaaatctgt ttggcagaaa ccatgttttc ttttttcact
     7201 gggacaaatt tttcatggac gaaaaacaga ctcaacaaga agacgaaaaa taagatgatc
     7261 cctgtaatga aaaaggtagg gcgatagcca aacgctgaag cagagatccc gccaagaagc
     7321 ggtccaagta atgttcctgt aacagaacca gttgctaagg ttccaagaac ttgaccactt
     7381 ttttcttttg gcgtcccagt tgcgacaagg gcagtggcat tactgatata accagaaaaa
     7441 atcccttgaa gcagacgcaa tgcgactaat tgatacacac tagtcacgca acccatcaaa
     7501 gaaatgacga ttgccattcc taatgaagca cgcaacagca tcaattttct tcctttttgg
     7561 tcggctaatt ttccccacca aggagagata agcgtagtca ccaagaaagt agatgaaaaa
     7621 gtcaatccgc tccaaaagtt caattgtgac gtactataat tacctaacgt atctatgtaa
     7681 agtgacataa aaggcatgac taaactaaat ccgataccag ccataaatgt tccaaaccat
     7741 aagacaaaca agttttgttc ccaagtttct cttttcctaa ataatttttc ctttattctt
     7801 gcagccaatt agactccttc tttctgaaaa ttttcatcta cagctcttta ttataaacta
     7861 caatatgtag tttatcaatg aaagtctctt ttttctcttt ctttttcgtg gtaaactgaa
     7921 agagtgaagg atttatttta tgagaggaaa catggaaatg aaaaaaagaa atctgtctca
     7981 agagaaaatc atcgactgct ttcgagaact agcagaagag atggatgttc agcaaataac
     8041 ctttcagcat ttagccaaac gcttggatat caaggctccc tcgctgtata accatttcaa
     8101 aaatattcgt gatgtcaaaa cagcgctaac agcgaaactt ctgcaggaac ttaatgacca
     8161 gctgcgacgg gctcttgtgg gaaaaagtgg tcaggaagca ctgcagatct atgcagaaac
     8221 ttatcgatcc tttgcttttt caaatcaagc agtgtatgag ttactgatca gtgtaccgca
     8281 taccaatgaa gctgttttat tagatggaat ccatgaaacg aatcagatca ttttgcagat
     8341 atttgaggct tttcaattaa cgaaagaaga aaaagttcat cgaagcaggg aacttcgtag
     8401 tatgatccac ggttatcttt ccttacgttt tttaggatac tttacaaaag aaccaacaat
     8461 cgatccagaa gacagctatt tttggatgat caataacttt atcgctactt tgccttaaac
     8521 aatcagattc atttgtaaca attttaagat gttcattaaa tgaccctttt ataagagaaa
     8581 attcttgcaa ctttcttgaa aagtgactaa gatgagtcta gaaaagctga aagaaggagc
     8641 acgagtatgt atcaagtaat taccatgttc ggggacaatg agccttggtg gttttttgaa
     8701 gactgggaag aagatattga agaagaagaa acattcgagt ctttcgaaga agcacgacaa
     8761 gcgtacgaaa aacaatggaa cgaaatccat caaaattatg aatatatcaa tgcaaaaagt
     8821 aactttctca gcgccttttg gaatgacgga gatgagcgct ggtgtgaaga gtgtgacgat
     8881 gatttacagc aatacaaggg acttgcgttg ttgaaaaatc atcagcccat cacagtagaa
     8941 agcagaaagg aattttatga aacaactaat tctagcggaa aaaccaagcg ttgcgaaaga
     9001 cctaagcaag gtgcttggtg ctaatcaaaa gcacaaaaat tattatgaag gacccaaggt
     9061 gatcgttact tgggcccttg gtcatttatt aggattgaaa atgccggaag atctgaataa
     9121 ggaatggcag agctggcaaa tggacacgtt gccaatgatc cctaaaaaac taggaatcaa
     9181 accacttcct aaaacaggac atcaattaaa agcaatcaag cagctggctc agcgaaaaga
     9241 tgtctcagaa gcagtcattg cgacagatgc cggaagagaa ggggaacttg ttgctcgctg
     9301 gatcttagag tatgtgcatt tcaataaacc agtgaagcgg ttatggatct cttctcaaac
     9361 agataaagca atcaaaactg gattcaagca actaagacct gcgaaagaat atgatgcatt
     9421 gtatgaatct gctcttgccc gggcaaaagc ggactggctg gtaggattga atgtgaccag
     9481 agccttaact gtgaagtacg cggataattt atcagcaggc cgtgtgcaga caccgacact
     9541 agcattagtg agagaccaag aaaagaaaat cgagcagttc cgtccacaaa cgtatttcac
     9601 gatcacgttg caagctggaa aagaacaagc caaaatgctt caaaaaaatc aatttgcttt
     9661 aaaaacacaa gaagaagcac aaaaacttgt tcaattgatc agtacaagca aaggaactgt
     9721 cacagaagtc caagaaaaat taaaaacgga atctgcgcca ttgccttatg atttaacaga
     9781 gatccaaaga gaagccaatc aacgctatgg ctattctgca aagaaaacat taagtcttgt
     9841 acaaagtttg tatgagaccc ataagatcgt tacttatccg cgtacagaca gcaaatatct
     9901 gacaaatgac atgaaaagta cgatgaaaga acgattgcaa gcagtcagcg actttgctcc
     9961 tgaggtcaag cagtatttga aaaacggagc catcgtccaa cagacaaaag tattccaaga
    10021 cagtaaagtg accgatcacc atgcactttt gccaactgaa aacagagcac gttatgaaaa
    10081 attaagtaat gaagagcgaa aaatctatca aatgattgtc tctcgttttt taggactgtt
    10141 tgctcaaccc cataaagtca gccaaacaaa agttacggtt gaatttggta aagaacgatt
    10201 catttttcgg caaaatcgtg tcgtccaagc aggatggaaa ggcgaaactg aaactgagac
    10261 ggaaacagtc aaatgggaaa aaggaatgag aatcaatccc gatttcgcca tcaaaaaaga
    10321 actatctgca ccgcctaaac cattgtcaga agcaagcttg cttggtcaaa tggaaaaata
    10381 cagtctggga accccagcta cgcgtgcaga gattatcgag aaactaatca aatctgaact
    10441 gatggaacgt acccctcaag gactgcaagt atctcctaaa ggtaagcaat tattagtatt
    10501 ggtaaatccc tctttagtaa caccagattt gacagaaagc tgggaaaaag accttgaagc
    10561 tattgctgca ggaaagaaac aagcaggaac cttcttgaat ggaatcgaaa aagaaacaaa
    10621 acgcttggtg aatgagatca aatcaagcaa acaagaatat caagatttct cgattaccca
    10681 aaagaaatgc ccagaatgtg gggccaatct gcgcgagaaa aacacacgtg acggcaaaat
    10741 ctatgtctgc accaattcag attgcaacta ccgcagaaga aaagatccaa aagtctctaa
    10801 tcatcgatgc ccgcaatgtc accggaaaat ggaaatcatc gaaggtaaaa acggtgctta
    10861 tttccgttgt aaatacgatg gaacgacaga aaaattgctt gataaaaaag agcagaagaa
    10921 gaaaatgacc aaacatgaag aacgacgctt gctgaaaaaa tatgcacaag cagaagaacc
    10981 agaagaaagc ccattagcag cagcactaaa agcagcaatg aagaagtaat aagatatgaa
    11041 agaggttgtg atagaagtgg tcagcttcaa gaaataagaa ggaatttacg aaaattgctt
    11101 ctcaaatttt tgtgaaattt cggcttattt ccgaagaagc tacttctgga acaccattta
    11161 ttcggaaaaa aggactgtga caagactttt gtcacagtcc tttttcattt aaaaaaatgt
    11221 ttacttcatt tgacgtatat tattggtgag gcttcaattc atttggcttc actttttctg
    11281 tttctcttat gatataaaca ggacgctgtt tcgtttctaa gaagattttc ccaatatatt
    11341 tcccgatgat tccaagacat aacaattgga ttccaccaat gaacaggaag atcgagacca
    11401 tggaaggcca tcctgatgta ggatcaccaa acaacaatgc ccgcaaaata atgaacaaaa
    11461 ttgcaatacc agaaccgaca caagacaaag cgccgataaa tgaagcaatc gttaacggtg
    11521 catccgagaa gttcacgata ccatcaatcg agtagttgaa taatttccag aaggaccaag
    11581 atgtttcgcc agccacacgt tcgcggtttt cataagaaat atattctgtt ttaaatccga
    11641 cccagctaaa taatcctttt gaaaatcggt tgtattctgt taattctagg acggcatcca
    11701 ccatttgacg agtcatcaag cggaaatcac gagcgccatc gaccatttct gtatcgctga
    11761 ttttattgat caactggtag aattttttcg caaagaaact gcgtatcgca ggctcgcctt
    11821 cacgagtcga acgccgggtg ccgacacaat cgatatcaga agtagtgatc cggttgatca
    11881 tctcaggaag cagttcgggc ggatcttgca gatcaacatc catcacggtc actaaatctc
    11941 ctgtagaagc ttgcaatcca gcgtacagag ccgcttcttt cccaaaattt ctggaaaatg
    12001 agacaaagcg gacataagga taagctgagg ctagcttctt gattgctgct aacgtaccgt
    12061 cttttgatcc gtcgtcaata aacaaatatt cgaactcatg ggcacctttg aatttttcaa
    12121 cctcatcaaa aaaaagagga acagtttttt cttcattaaa acatgggaca acaattgata
    12181 ctttcatcgt ttctatcctt tcaatcgtat gtttatatat ggtttacatt caaaatttaa
    12241 taaaccttaa ataacacctt taaaatttga ttttacgtaa aagaacaggt attctttttg
    12301 taaactaacg tatttagttt accatgtttc ctcggtttat caaactttta agaacaattt
    12361 ttattagttg gcaagtagtt ttggatagag aaaaaggagg aagttccagt ctattgacta
    12421 ggagcaaaca aaatgaaaat acacacacct atcggctttt tatggttaga tgcagatgaa
    12481 tccggattga ctgctgtatc ttttcagcca atttcagaaa gcgaatcagc gaatcagcga
    12541 attttaacgc gcacacaaga acagtttgaa gaatattttg cgggaaaaag aaaagaattt
    12601 gatgttcctt tatctataaa gagaggtacg ccttttcagc aaaatgtctg gaaagctttg
    12661 tcgaccattc cttatggaga aatccgaacg tataaagaag tagccgttgc tgttgatagt
    12721 ccgaaagctg tgcgagcgat tgggcaggca aatcgaacca atccattgcc aatcgttatt
    12781 ccttgtcatc gagtgatcgg gcaaaatgga aaactgacag gatatatggg aaacgcaata
    12841 gatggggttg aaatcaaacg ccaatt
//
LOCUS       DAZBTF010000077        12733 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000077 DAZBTF010000000
VERSION     DAZBTF010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.077"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACHGNS_002321"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACHGNS_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFN6938244.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="ACHGNS_002322"
     CDS             complement(323..892)
                     /locus_tag="ACHGNS_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFN6938245.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="ACHGNS_002323"
     CDS             1078..1980
                     /locus_tag="ACHGNS_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFN6938246.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACHGNS_002324"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACHGNS_002324"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFN6938247.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACHGNS_002325"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACHGNS_002325"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFN6938248.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="ACHGNS_002326"
     CDS             complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="ACHGNS_002326"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFN6938249.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8548..8733
                     /locus_tag="ACHGNS_002327"
     CDS             8548..8733
                     /locus_tag="ACHGNS_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFN6938250.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8795..9991)
                     /locus_tag="ACHGNS_002328"
     CDS             complement(8795..9991)
                     /locus_tag="ACHGNS_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFN6938251.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10161..11597)
                     /locus_tag="ACHGNS_002329"
     CDS             complement(10161..11597)
                     /locus_tag="ACHGNS_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFN6938252.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11890..12699
                     /locus_tag="ACHGNS_002330"
     CDS             11890..12699
                     /locus_tag="ACHGNS_002330"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN6938253.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3801 a         2672 c         2250 g         4010 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttgctgtcat ccacccaata agcatctgac agaactactt
     5101 ttcgttgcgt tttttcgtga tacataacta ctggtatata tcctgggtgt ttctgaaaaa
     5161 tcacttgtaa ttgtttcatt atctcgggat cttcccgttc ttctgtaata cgcaaatagc
     5221 aagtttgtga aggatactgg gcttctaact ggttaggatc agcaaagctt tctccaatta
     5281 gttgcagttc ctgattatat ttgctgattt cgctccgccc ttgaacaaag atcactcgat
     5341 caacggcaaa caaattacga tatttacgat aaagagttgg aaaaactgtg acagaaattt
     5401 ctccactact gtcggttcct tcaacaaagg ccatcagttc tccttttttt gtccgaattt
     5461 ctctaacttc cttgatcatc actaataaat tgactggttg attagggacg atatctgtaa
     5521 tcagctgaac ttgtttagct aaacgaagac gatcaaaccc ttctgcagga tgacctgaca
     5581 aataaatacc taagtactcc tcttccagct tcaagcgttc ttctagtgta tagtcagcga
     5641 cttcttcttc ttttaatgcc atgattccca gcaagtcaag acttccacca ctgtaaacga
     5701 tattttggat ttttccttcg agatcaagca tcaattgttt cctgttagga gataattcat
     5761 caaaaactcc tactgctgtc agtgattgca gtaactctaa tttcagccaa cgcttatcga
     5821 tccgtatgag gaattgatcc actgactgga acgggccatt ttgttttctt tcgtttaata
     5881 tatcttctac aaaatcacgc cgaatacctt ttatcgctgt cagtccgaat ctgatttctt
     5941 ccttattgac caactcaaaa ctggaattac tttgattaat ggacggcgag gaaagtttga
     6001 gtttattttt acgtgcttct gcaatatatt ctttcaattt tgatgtgtta tgccgaacag
     6061 aattcatcag gctggtaaag aaagcacctg gatgatgggc ttttagatac gccatttgga
     6121 atcccacgaa tgaataagca aatgcatgtg accgattgaa tccatagttt gcaaattttt
     6181 cgatatattc atatacggtc gctgctgttt tttccgaata tccctgttgt aatgctcctg
     6241 atacaaaatg cgacttttct tcatctaata cgtctttttt ctttttactc actgcccgtc
     6301 gcagaatatc tgactgccct aatgtaaatc cagccatagt cgaggcgatc tgcatgattt
     6361 gttcctgata gacgatcact ccatatgtgt ttttcaaaat cgggatcagt gaatcatctg
     6421 ggtagcgaat ggcttctttt cctttcttcc tggcaatgaa ggtatctata ttttgcatag
     6481 ggcctggtcg atacaatgca ttgactgcgg caatatcttc gatgttttct ggatctaatc
     6541 ttcgtaaaac gttccggata ccagcagatt caaattgaaa tactcctgag gtttcgcctc
     6601 gttgaaacaa gagcagcgtt tttgcatcat ttagcgggat ctcttttaat cggatctcat
     6661 acccttctac tcgcttaaca gctttcaaag taaaatcaat aatcgataag ttgcgtagtc
     6721 ctaaaaagtc cattttcaaa agaccgattg cttctacctc attcatcgta tactgggtca
     6781 gaaaagcttc atcagaaccg cgttggagcg gaactagttc aagtagattc ttatcgctga
     6841 taactacacc tgctgcgtga gtcgaaacat gtcttggcaa tccttctaaa atcactgcag
     6901 ttttgaataa aagttggttt ttttcgcttg cctgaactaa ttctactaat tgatgcgaag
     6961 ttttataagc ttgggtcaat gtgattttca attgattggg tattgctttt gaccagcggt
     7021 ttgcttcact ttgcgataaa ccaaacactc tagctgcatc tctaagaacc atttttgctg
     7081 ccattgtgcc aaatgttgcg atttgtgcca tatgatattg tccatacttt tctcgaacat
     7141 aattcaatac ttcttcacgt cgattgtcag gaatatccaa atcgatatct ggcatcgtaa
     7201 agcgttcggg gtttaaaaag cgttcgaaca gcaaatcata ttctaatgga tccacatctg
     7261 tgatttctaa cacataggct actaatgaac ccgcagcaga accacgacct gcaccggtaa
     7321 cgattttgtt tctatgagca aaagccatta catcccatac gatcaagaaa taatcatcga
     7381 atcccatttg atggatgaca gataactcgt agtccaaacg ttcttggtat tcctttgtat
     7441 agtcagtgat tcggcgttcc acttgcgaat agcataactc tcttaaatat tctccagctt
     7501 gttttccatt aggaactgga taatgcggaa gtaatttctg atggaactgg aattcaaatg
     7561 aaatcgattc agctaaacgc tgggcgccta ttacagcttg ggaaagaccc gctttttcga
     7621 atcgtttgat catttcttgt tctgaaagaa gatagacatt tgctgttagt ggtaaagatg
     7681 cagataaatt ttctaaaggt atttggcttc cctctttgat atgtttcatc acttcaagtg
     7741 caaaaagttc ttcttgttgt aaactagcga tgggatgtaa tgcacaaagc gaataatttt
     7801 cttttatcac atactgatac cattcaggca attgatcagg cgtgttttct gtcaagccga
     7861 tataaaaatt tctagtaaaa acattttcaa gtaaaaactg ccaatgaccg aaaaagttat
     7921 cctctttatt gaaagcctca gaaacagaat gatttgccgg aacaacaaac gctagatggt
     7981 cggtttctgt catttctgca agttccaatt tttcttggat catttttcgg ctggaaagtt
     8041 tcatcagctg ctgataacct atttcatctt ttgcgtacgc aagcaagcca taattttttt
     8101 cttcataagt ataactaaga tgtaatccaa tgattggctg tactccttct tctacacaaa
     8161 tacgatagaa ttcaggtgca ccgtataaga catctttatc cgttatggct aatgaagtgt
     8221 agccttgttt ttttgcttct tgtacatatt ctcgaatacg tatcgtactt tgtaaaagag
     8281 aataagaagt aattgtataa agttgtggga aaaacatctt gcattccgcc ttttttttcg
     8341 ttaaaattca ctagcgttaa aaggtttgat ccttttactt tcattaagat agcaggcgct
     8401 gaagttgatt attccacttc atttgcttgc ttgtaatctg taggaatgag ttctttcgca
     8461 agaactcaaa ttttattgag atttggtctc gtttctttac attcaaagaa aatctggtaa
     8521 aattaagata gaaaagaggt gttctttatg cgatatatcg ttacattatt ctgggctgtc
     8581 gttctaggtc aagtagtcgg ttatattggt gctgcattaa caagcggaac atatgatttt
     8641 actttgacaa caatcatttc attcattgcg ggtgtcatta tccttttgat tggtgcagtt
     8701 gcgccaagaa aagaaacctc tgcccattcg taaaaaaaca gccaagagct tgagatttct
     8761 caagctcttt tttattttct actcctcacc accattataa tctgctgcga agaactttac
     8821 gaaagttgaa taaagaaaga agaatatttg acagaacaaa caaacttgtc acaataaaaa
     8881 cagcactgta attataagca tgcgcgactg tcgatcctag catgggacct aaaacttgtc
     8941 caaagttgct aaacatctga ttgtagctat agattcggct gactccttct tgaggcgtaa
     9001 tcttactgat cagtgtattg acagaaggca tcaaagcgcc agttgaaaag cctaaaataa
     9061 agcgaagtat acctaattgg agcggtgtag tgacgaatgc cataggtaaa taacagcaaa
     9121 atgataaaat gagtcctcca attagaactt tttgattccc aatcttatcc cctagttttc
     9181 ctagtttcgg tgaagaaaag acagcggaaa caccagctat cgaaacaatc aagccgctga
     9241 caaaaaggat attttctgta cttccgctca attcacgaat atataaggtc aaaatagggc
     9301 tgatcgtagt gattcctatc tgtaaaatca aggtcgtaat gaagagaccg atcaaaatag
     9361 agagatgatc catttttgaa aaaatttctt tggtgcttat cagatctttt ttctcgattg
     9421 gatggaaatc ttctttgact aagaaaatgg tcaataccgt tgtgatcatc aacaaagccc
     9481 ctgtgatcaa gaatacattt tccataccaa accactgagc cagtgcaccg cccattgaag
     9541 gtccgatcag atttccagca atcgctccgg tagacaatgt tcctaacgcc catccgctct
     9601 tttctctagg tgcctgtgaa gcaatcatcg ctgtggcatt tggaatatat ccagacaaaa
     9661 tcccattcca aaaccgcatg atcaaaagcc aatacgcgtt tggtacaaaa gccaaagatc
     9721 ccattgttaa tgtcatccct gctgctgcac ggatcatcat gagttttcgg ccttttcgat
     9781 cagctagatt tccccagatt ggtgcaacca cggcagatgc aaaagcagtc acagagattg
     9841 ctagtcccga aaacaattcg atttgatctt ttggtgttcc tagttgctca acataaactg
     9901 gtatgaatgg cataactaaa ctaatacttg caccagtgaa aaaacagcca atccacgcaa
     9961 tcactaaatt ttttctccaa ttaatctcca tctatcatcc ctctttcgtc tcttttatca
    10021 tacctatttt cgaataaaca agcaacgatc tcgccagtaa aaagagaaaa atcccgcaaa
    10081 ggtcatttta tatgtaacgt tctttttatg taaaacaaaa agagatcaga acaaatactg
    10141 ttccaatccc ttctctttag ttaaattgtt gctagaatga tgaagttgat aatgaataaa
    10201 atatcaacga tccacaaagc aacagaaact tgattgtatt ttcctttgat tactttcaca
    10261 attgtataga aaataaatcc tgctgcaatc ccataggaaa tgctatagca caatcccata
    10321 aagatcgatg caaagaaggc cggtaaagct tcttccatat ctagccattt gatgtctgca
    10381 aaagaagcca tcatcattac tccaactaaa atcaatgctg gcgcagttgc ttgatttggt
    10441 acaatggcga tcagtggaga gaataaacta cttaatgcga atagcactgc cactactact
    10501 gaagtcaaac cagtacgtcc accggccccg atcccagctg cagattcgac ataagttgtt
    10561 gtattcgatg taccaaagat agcccccaca gaagtagcga tcgcatcggc aaatagtgct
    10621 ttatccattt ttgttttgaa cccacgacca tcttccaacg cgatctcgtc ttcttttgag
    10681 aaaatacctg tgcggcgtcc tgtaccgata aatgttccga tcgtatcgaa agtatcagat
    10741 aaactgaatg cgatgatcgt catcaatact tgaggaatct tggaagaatc actgaataaa
    10801 gattgcatgc cttcagaacc aaatgctgca ccgaaagttg ttcctaattc tttaaatgaa
    10861 ttacctaacg aattcgtttg ccaatcaatg gaacttaaat caacaacacc cataaaaata
    10921 ccaaggactg tggtcgctac gataccgata agaatagccc cacggacatt ttttacaact
    10981 aaaatggctg tcagtaccaa accaatcaca gccaataaga taggagcgtt gtcaaagttg
    11041 gctaacgcag ggacgattcc accatttatc gtaacatttg tcgcttttcc gccttcaacg
    11101 acagagctcg aaatagctga ctggtctgct gaaaaagata agaatcctgc atttttgatt
    11161 ccgacatacg caacgaaaat accgatcccg ccaccaatcg catgttgaag actttcggga
    11221 atcgcccgaa taatcatttt tcgaatattt gtgacagtaa taaaaatatt gattaatccg
    11281 caaataaaca ccattgccaa ggcttgttgc caagaataac caagcccaaa aacgaccgta
    11341 aaagtgaaaa atgcgttcaa tcccatacca ggtgcctgag catatggtac atttgcgaac
    11401 aatcccatta ccagcgtacc aataatcgat gcaatgattg tcgctagaaa tactgcttgg
    11461 aagggcatac ctgatgctga caaaatcgtc ggatttacga ataagatata actcatggca
    11521 aagaatgtcg ttactcccgc cattatttca gttgatacat ttgtattgtt tttctttaaa
    11581 ccgaaaaact tttccataaa aagttccact ccctgaattt ttttctgatg gaaaactata
    11641 ataataaaaa ataagtaaaa tagggcaaat ccatcgaaca atttcaaatt atatagacgt
    11701 ttcattcgct tttcaacgtt tttttaacaa aaaaagcgaa aataatactt aacgttcgtg
    11761 tttagcttat gtttagctat ttatatagcc ataactgtag tttttgttag gaatagctga
    11821 ttgctcttgt taattaccta aaatatgcta gactaaaaca aatgctagtt gaggaaggaa
    11881 ggacccttat tgaataaaaa attaattgcc atcgatctgg acggaactac cctcaatcaa
    11941 gattcgatga ttacatctaa aacaaaagag actctaaaaa aagcaatcaa tgccggtcat
    12001 catgtcagta ttgctacagg acgcccattc cgaatgagcc atcagtttta ccaacaatta
    12061 gaattaacaa caccgatggt taattttaac ggcgcattag tacacatccc taatcagcac
    12121 tgggatggtg aaagagaaac gttgatcaat cgtgaaatcg cttttgaaat cttatcgcaa
    12181 aaaaagcagc taaatttaga ttttatcgct gctgaaaatc gtgatacttt ttttattgac
    12241 agttttgatt tctttgatga aaaaattttt gcttcttctc gtccaggaga gaagaatttg
    12301 ctttctccta aaaatttgac gacaaatcct acatctttgc ttgtccgaac agacaaacgt
    12361 tttgcaggag ctgtatctgc tgaattaaca cgtcaatttg gcagttatgt cgatgttcgg
    12421 acatggggtg gacctactgc catcttagag attgtttcaa aaggaatcca aaaagcaaaa
    12481 ggcgtgcaag agattgctaa ctacctgtct atcgatcaaa aagacgtcat tgcctttggt
    12541 gacgagcata atgatttgga attgttggat tatgcaggtt ggggagtagc tatggctaac
    12601 ggtactgacc aactcaaagg cattgcaaat gatgtcactc ctttgagcaa tcaggaagac
    12661 ggtttagctg tttatctgga aaaactactt aaactataat agagaattag attccacaaa
    12721 aaagaggttg tga
//
LOCUS       DAZBTF010000078        12184 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000078 DAZBTF010000000
VERSION     DAZBTF010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.078"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(146..310)
                     /locus_tag="ACHGNS_002331"
     CDS             complement(146..310)
                     /locus_tag="ACHGNS_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938254.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            605..1933
                     /locus_tag="ACHGNS_002332"
     CDS             605..1933
                     /locus_tag="ACHGNS_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFN6938255.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2135..2968
                     /locus_tag="ACHGNS_002333"
     CDS             2135..2968
                     /locus_tag="ACHGNS_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFN6938256.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2984..3721
                     /locus_tag="ACHGNS_002334"
     CDS             2984..3721
                     /locus_tag="ACHGNS_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFN6938257.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3876..4844)
                     /locus_tag="ACHGNS_002335"
     CDS             complement(3876..4844)
                     /locus_tag="ACHGNS_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFN6938258.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5076..5909
                     /locus_tag="ACHGNS_002336"
     CDS             5076..5909
                     /locus_tag="ACHGNS_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFN6938259.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5943..6782
                     /locus_tag="ACHGNS_002337"
     CDS             5943..6782
                     /locus_tag="ACHGNS_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN6938260.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6808..7368
                     /locus_tag="ACHGNS_002338"
     CDS             6808..7368
                     /locus_tag="ACHGNS_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN6938261.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7567..9288
                     /locus_tag="ACHGNS_002339"
     CDS             7567..9288
                     /locus_tag="ACHGNS_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFN6938262.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9453..10595
                     /locus_tag="ACHGNS_002340"
     CDS             9453..10595
                     /locus_tag="ACHGNS_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFN6938263.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10611..11822
                     /gene="thiI"
                     /locus_tag="ACHGNS_002341"
     CDS             10611..11822
                     /gene="thiI"
                     /locus_tag="ACHGNS_002341"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFN6938264.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4152 a         2044 c         2531 g         3457 t
ORIGIN      
        1 tgccggagta aataaagctt atgcaaagaa aaagaaatcc aatcacttgg gtttcttttt
       61 cttttattat tatgatgaat caagctttct gtttttgtca cgtatgttgt aatctttacg
      121 aaagagtaga gttggctact cataatcaac catacttccc atcatcgcta aatacgaaat
      181 aaatcagcat tcctacacca atcaaagcac aagctgcacc aagaaaaggg atgacattcc
      241 caaagttatc tgtattcata agtatgccac cacccacaaa caaaaagccg ccagcaatca
      301 ggtttttcat tttattcatc tcctttctta cgttatagta tttccttctt tcaatgagta
      361 ttttgaaagc tatcttattg tatgatgttc ttctaggcct cttctttatt atacaggcca
      421 ttttcgttat ctcatagaaa tattcctttt tatctgaaaa ctattgtata ccgtatgaca
      481 tttttcttca tgaagttgta agtatattct cattcaaatg aaaatttcat catagaagag
      541 attgtatttc taaattctta agagtaaaat acgaagtata acttggattt ggaaggatga
      601 ttggatgaaa gctattacgc ctgatatgac aacaaaattt aacaaacagt ttcaagcaaa
      661 tatcaaacag caggcattgc agcgggcagt tgtgaagaac ggaattactg cttctgctga
      721 aaatgtttca gcacatgtgg agaatacacc ggtattttct atagatttgg ctacggggaa
      781 agtggctaat caaatgcaat cggggcgttg ctggatgttt gctgcactga acacattccg
      841 tcataagatg ctgaatcaat tccatttaaa agagtttgaa ttatcacaaa attacacttt
      901 tttctgggat aaatatgaaa aggcaaatta tttctatgaa aacatcattg caacaggaaa
      961 agaggcaatc gatagccgga aagtagcttt cttactcgct actcctcaac aagatggcgg
     1021 ccaatgggat atgatcgttt cattattcca aaaatatggc gttgtgccta aatcggtgat
     1081 gccggaaagt gcgaatagtt caaattctcg agatttgaat aactacttga acaaattact
     1141 gcgtaaagat gcagtagtgt tacggaaaat ggttgctcaa ggagaaacat tagacgctat
     1201 tgaagaaaag aaagaagaaa tgctagaaag catttataac ttcttagcta tctcattagg
     1261 aacacctcca aaagaattcg attttgagta ccgcgatgaa gaaaagaact atcatcttga
     1321 ccgaggactg acaccacaaa ttttttatga taaatacatc ggtgtaaaat tagatgatta
     1381 tgttagcgtg atcaatgcac caacaaaaga caaaccattc aatcgttctt atacagtaga
     1441 gatgctagga aatgtcgttg gcgggaaaga agtcaaatat ctgaatgttg atatggaaac
     1501 tttcaaaaaa ttagcgattg ctcagcttga agaaggagaa tctgtttggt ttggttgtga
     1561 tgtaggtcaa tcttctacta gaactagtgg catcatggcc ttagatgctt attcaatgga
     1621 tgatctattt gatacggatt ttacaatgac taaagctgaa cgtttagatt ttggagaaag
     1681 tctgatgacc catgcgatgg tattgaccgg tgttgactta gtagacgggc aatcaacaaa
     1741 atggaaagtc gaaaatagct ggggcgaaaa agttggaaag aatggcttct ttgttatgag
     1801 tgatgcctgg atggacgaat atacgtacca aatcgttgta agaaaagatt tattgacgaa
     1861 agagcaatta aatgcgttca atgaagaacc aattgttcta tctccgtggg atccaatggg
     1921 tgctttagca tagacgagta tttcaatgaa gagtatgtag taaagataaa aagagctggc
     1981 ttaagccagc tctttttatc tttaaagtcc attccctgtt ttttatggtc cctgtgctat
     2041 aattctaaat aaaggataag gattggaaat aatcctttta tcattaaaaa tatgatgacg
     2101 aagtagaata aaattgggag taggggggaa agtcatggat atccaaactg ttttatctga
     2161 tctgaaatta aatggtaaag tgattccagt tgttggtggc gatgttaatc agacctatcg
     2221 tataaaaacc gaacatcgtg cttatttttt aaaaatccat ccgaatgtaa aaaaaggatt
     2281 ttttgaagca gaagtagacg gattaaaaga actgtcagca ttcgtacgtg taccggatac
     2341 gtatatgcta ggtgaaacaa gtgaaggcgc ttatttgtta atggaatgga tcgagccggg
     2401 aaaaggggat caacgagatc ttgctgcagc tttagctaat ctccatcagc aaacagcgcc
     2461 acaatttggt tttcgtaaag ataactattt gggtacactt gttcagaaaa attcttttga
     2521 agaagattgg tggacttttt tctttaaaga tcggcttgaa tcccagattt ctttggcaga
     2581 agaaacgaat cgatggaatg tacagcgtca agaaaagtat ctgcgattta aagaacgtgt
     2641 attgaaatcg gttgaaccaa aaaagatcac tccacgcctc ttgcacggag acctgtggag
     2701 tggtaatgtg ttctttgacc agcaagggca tccagttttt gtcgacccag ctgtttctta
     2761 tgggaatcgg gaacaagata ttgcaatgag ccagttattc ggtggtttcc gaccggaatt
     2821 tttagatgct tatcaaacta tatttccatt agaaaaggga tggaaagacc gactgccgat
     2881 ttaccagtta tattatcttt tggcacattt gaatatgttt ggagaaagtt acggttccca
     2941 agtagatcaa ttactagaaa atttttaaga ggagggcgtg tttttggcaa aaaaatatac
     3001 caaaaagcat gaagtttcat attatgagtg tgacatcaac caaacgatga catttccttc
     3061 gatgttagga gtagttatca aaacgtctga agatcaaagc gaccagttgg gaagaggcag
     3121 tgaattcgtc aatagctttg gacttacttg ggtcatcacg aattattcca tggagattaa
     3181 tcgtctccct agagtcggtg aagtaatctc agtaacgact caagccatgg agtacaataa
     3241 gtttttctgt tatcggaact tctggatcca tgatgaagat gggaaagaat tagtcaaaat
     3301 agaatctgtt ttcgtattga tggattttgt gaatcgaaaa atgagcagtg ttaatgaaga
     3361 gatcattgct ccttttgaga gtgagaagat caagaaaatc aagcgtcagg aaaaaattga
     3421 aaagattgat tcgggcttca tgttgccata tcgtgttcgt ttttatgata tcgatagtaa
     3481 tcagcatgtt aataatgcga tgtattttaa ttggatcatt gacgtattag gatatgactt
     3541 tttgactacg catgttccag aaaaagtaat tattcgattt gataaagaag tagagtacgg
     3601 gaatgagatc gaaagccatt ttgaacaaat ccctatcgaa aatggtgtca aaacaaaaca
     3661 tgaaatccga atgcaagaac agctgtattg tgaagcaaat attatatgga aaaataaata
     3721 aaaaagaggt tgtgacagaa gggggcaact tcaagaaata agaagaaatt tacgaaaatt
     3781 gtttctcaaa tttttgtaaa ttttcgactt agttttcgaa gaagctattt ttgtcacagc
     3841 ctctcttttt aagtttagct attagataat gatgtttagt tttggataaa cgaaatcaat
     3901 tcttcacccg acaaaccata gtttacagca atcaatagtt ttaaacgtgc tttttgacta
     3961 ttaatactgt tacagaaaat aatccccatt ttttctagtt cttttccccc gccttcataa
     4021 tcataaacag gctctgcgat tccattgaaa caacgagaaa caagtactac agggattttt
     4081 tggacaagaa gcttttccaa agcagctaag gcttgtggag ggagattccc cgcacctaaa
     4141 gcttcgatta ccaaaccgtc tactttcgta tgggcgatgg cttccaataa gtccccttgc
     4201 atcccagcat atgctttaac gatcgggata gtcccatcta cagcggaaat gtccagtcgc
     4261 tttgtttcta aaagttcctg caagaataaa attcgattct tagttactaa gccgatggga
     4321 cccaatgtag gtgttcggaa agtagcaaca tttgtggtat gggtctttgt cacgtagcgt
     4381 gcagaatgga tctcatcgtt catgaccacc aaaaccccct tatctagcgc ttcttcacaa
     4441 cttgcgacac gaatcgcact ttcaaaatta taaaggccgt cacttccaag ttcattactt
     4501 gaacgcattg ccccagtcaa aacaattggt aatttgttgc caatcgttgt atcaagaaaa
     4561 aaagcagttt cttctaatgt atctgtaccg tgtgtgatca ccactccgga tatacctgat
     4621 gcataagcgg acaagatacg ttcttttaat accaacatgt gagaaggcat gatgtgtggt
     4681 gagggaagat tgaagatatc ttcaactaat aattctacat gcttggggat tgtgatttct
     4741 gcatctaata atgggtttgc cacatcagga ctaacaccgc ccgtgagcat gttttctttc
     4801 atggcgatcg ttccgccggt gtgtagtaca agtatttttt tcattttaca gacttcctta
     4861 cgaataattt tgcatagccg ttaaatctta ttgtatacta tataaaagag ttttgctatc
     4921 agtgaagaat aacgaatagc ctaacttatc ataaaaaagc tgaccttatt aaaatggaaa
     4981 agactcaacc ttgacctcct aatacaatgg agtatctggt tgagtttctt ttacaagaaa
     5041 gcagccaagg aaatcaatca cgaaggtggc cggaaatgat cagaatcgga ttaacatcgt
     5101 ttaaagaaca tgacaaatta actggtaaaa agaataacac tctttatgaa tatgcagggt
     5161 ttctaccctt agtggaatta gatactgcct attatggaat tccccgaaag tcctcagttg
     5221 agaattggat aagtgaagta cctgaatcat ttcgctttgt agtcaaagtc tatcaaggaa
     5281 tcagcggtca aggagactgg aagccttatt atcagaccga agaagagatg attgcgcatt
     5341 ttttagatgt tatgtcgcca atggtagatg ctggaaagat gttttgcttt ttagtccaat
     5401 tcccctcatc attcaaatgt acgaaagaaa atgtccagta cttgcgcaaa cttagaaagt
     5461 ggtttgaggg ttatcctgtt tctatagaat tgcgggatta ttcttggtac agcgatgaat
     5521 ttcttgaacg tacaagaaac tttatgtcta gagccgcttt ttctttagcc atcatcgatg
     5581 agccacagtt gctgaataca acgattcctt ttgatccatt tgtcaccaat ccgaactttg
     5641 ctctttttcg attccatggt aggaatgcgc aaggatggca agcaaatgat aaagagtggc
     5701 gtaaaaaaag aacgttatat cgttattcag aaaaagaact gcaagaacta gcacatgaag
     5761 tcaaagtagt tgattctaag acaaaagagg taggtgtgat ttttaataat aattctggtg
     5821 gtgatgcggc tgaaaatgca ttagcactgc aaaaaatatt aaatatcaag ccagatggac
     5881 tcaatccaac ccaaatggat ctgttttaag ggcttatttt attcaagaaa ggggcgaagt
     5941 ctatgatcaa agctattttt tttgatattg acggaacatt ggtcaccaat cgttcaaaag
     6001 cgttggaatc aacaaaacaa gcaattgaat atgctcggaa aaacggcatt ttatgcggtg
     6061 tagcaacggg gcgcagccca gtaaagatca aagaaatcat tgatgaattg gaattagata
     6121 tgtatgtcgt gtataatggt caactggttt ttactgcaga tcgcacgatt atcgaccacc
     6181 cttttgaaca aaaagtattg gaacatattg ttgaatttgc agatgagaac catcgacaaa
     6241 tcgtttttgg tgcgagaaat cggttagatg gatcaacgac gatgctatta ggacaatcga
     6301 tttttatcaa gcgactagtc agctttttac cgcgtaaatt cccggtacgt ctgatgaaaa
     6361 aaatcctcca ggtttttagc cctcaccgac aaaaggatcg ttatgaaacg ttagagatat
     6421 taaaagaacc aatttatcaa tgtatattgc tcagtccgga gtcagaacag aaaaagttag
     6481 aacatcgctt tcccgagtgt acgtttcagc gttcaaatac gtatactgta gatatcatcc
     6541 caaaaggtgg ttcaaaatta ctaggcatcc aagctttcgc aaatgctgtc gaaattgaaa
     6601 tggaagaaat tatggcattc ggcgatcatt acaatgatat cgagatgctg aaaggtgtag
     6661 gaattggagt ggccatgggg aatgctcaga tcgaagtaaa acatgctgca gactttgtga
     6721 ctcaatcaaa tgaagaagat gggatatacg aaggactgaa acacttcggt ctgatttatt
     6781 agtcaatgaa agaaggaaaa aatcagtatg gaagcagaga caccttttca aaaagtagaa
     6841 acattccaca aaacttttga tccaagaaga ccagagatac ccacggcttt tacagaaaat
     6901 gaagctcata cacgtgctgc gttcaaaata gaagaacttg ttgaatttgt tgcagctact
     6961 tcacctaata aagaacaact gcatgaagct gttatgctgc tgcacgaagc attagataaa
     7021 gcagaagaaa aaatacttgg taaaacacag tggggaagtg atccgctggt tgaacaagta
     7081 gatgcactga cagatatcct ttactttact tatggttctt tttcattact aggaatagtg
     7141 cctgatcgaa tattcgaaat cgtccatcag gcaaatatgg ggaaattatt tccagatgga
     7201 aaacctcatt atgaccctgt aacaaataaa gtgttgaaac ctgaaaactg gcagatggaa
     7261 tatgctccag aacctaaaat cgcggcagaa atccaacgtc agattcaaga agcgattgct
     7321 aagaagaaat ccgctgatat aggcatgtgc acaaatgaaa aagagtgatc agagtttcac
     7381 tatttttgtt atctttatac gatggaaaaa caaaagattt tcgtttaagt tagtgaatgc
     7441 atgaaaatag tatgctataa tctagaatag atgtcttttg cacgagagac gtattatttg
     7501 gatttatgcc aagcagaacg ccatctgttg cataaaagat gcatactgaa aacggaggaa
     7561 ccataaatgg gaaataatgt tatcatcgga atcgtcattg caatcattat agtcgccatt
     7621 gtcgtctttt tggttagtta cttgatgaag aaaaaaaacc aagatcgatt ggatgcctta
     7681 gaaaaacgca aggaagaatt atttgacttg ccagtgattg aagaagtcga tgatgttaaa
     7741 aaaatgcatc tggttgggca aagccaaaat acctttcgtg aatggagtca aaaatggaat
     7801 gacatatcta cttcctcgtt cgctgaacta gaaagtcaaa ttttcgaagt ggaaaacctc
     7861 aatgaaagtt ttcggttctt aaaaggaaaa aaagcagttg ttgaagcaga agctacaatg
     7921 gaccaaatgg agaaacaagt gacggaaatc cgtgaaggtc taaaggaatt gcgggaaacg
     7981 gaagaacgca actcactagc tgttcagcag gcacttgacg tctatgaaga actgaaagaa
     8041 aaagtcagag aagacgagca tgaatacgga ccagctcttt ttgaattgca aaaacagatc
     8101 aaagtgatcg aaaaagaatt tacgcagttt gtgactttaa acacttctgg agaccctgtt
     8161 gaagcccgcg aggttttgga taaagcagaa aaacatacgt atgaagtgga agctctgatg
     8221 aaaaaaattc caccgctgta tgaggagctc cataccactt tccctgaaca acttgaagaa
     8281 atcagtgata cttacgagaa attgatggaa gaacaatatg tattcccaga agaagatctt
     8341 gctgaagata tcgcaaaagt aagtcgaaga attgaaaatt cattagccaa tcttgaaaaa
     8401 acagaagttg aaacggtcga atttgaaaac cgtgaaacag ctgatttgat tgattcgtta
     8461 tatgatatct tagaacggga aatggaagct cagagatatg taaaaactaa ccaatcaacg
     8521 attgcagagt atatcaaaca tacaacaaag aataaccgtc aattattgat tgagctggat
     8581 cacacatctc aaagctatac attgaaccac aatgaaattg gccgtgtacg aggtttccaa
     8641 accgaagtgg aagaaatgga acgtcaaaat gaacagatga ttccacagat ccgtcaacac
     8701 gaaattcctt attcagaagt gcgcacgttc tataaaacag tcttcaaagt cttagaagat
     8761 atcgaaaagc aacaagtaga gatcgacgac tcattacatg aattgagaaa aggcgaaaaa
     8821 gaagcgcaag aaaagatcga tacattcgaa ttcaaattgc gcagtttgaa acgttttgta
     8881 gaaaaacaac gtttgcctgg cttgccgaat gattacctag aattcttttt tgttgctact
     8941 gaccgtattg aagaactaag tgttgtcttg aataaaattc gagtaaacat ggaagaggtc
     9001 aatcgtttag ttgctctatg tgaagaagat ctagaattat tagataagaa gacacatgat
     9061 ttagtagatg cagcagcatt aacggagcaa atgctccaat atgcaaatcg ttatcgtcat
     9121 acacacgaag aagtgagaga agcaattgat aaaagctact atttattcaa taaagaatat
     9181 cactatcaag aagcattaga cgaaatcggt acagcattag agcgagtaga acctggtgct
     9241 ttcaaacgca tcgaagattt ttattttaat catccagact tagtataaag caaaaaagtt
     9301 agtaaaccag caagagcaag acggattatt gtcttgttct tgtctttttt tgtatatttg
     9361 aaaattgaaa caagtgaaaa tttcacctgc tcttgaaagc accttggaca accgttataa
     9421 tagaaaagaa tttcaaaaaa gaggggtttc ttatgatata ttttgataac agtgcaacaa
     9481 ccccaatctt tccgcaaagc ttagatgcat atgtaaaaac cagtcagcgg attattggga
     9541 atccatcaag cttgcatgat ttaggcaatc aagctaaccg gttgttgcag caagcaagaa
     9601 agcagatcgc tgatttgatc catgtagaac cagaagaaat ctattttact tcaggcggaa
     9661 cagaaggaga taactgggta ttaaaaggaa cgatgatcga aaaaagagaa tatgggaatc
     9721 atatgatcat ttcaggagtc gaacatccgg ccgtcagcga gacagcagaa caattaaaag
     9781 aattaggaat cgaagtaagt atcgccccag tggataaacg cggatttgta cgtgtcgacg
     9841 aattgaaaga attaataaga aaagagacag tattagtctc tgttatggct gtgaataatg
     9901 aagtaggatc gattcagccc atccaggaaa tcagtgatct tttggatgat tttcctaaaa
     9961 ttcatttcca tgtagatgct gttcaggcga ttggaaaagt accattctcg caatggttga
    10021 cagaaagagt tgattttgca acattttcag ctcataaatt ccatggccca cggggaactg
    10081 gtttcattta ttggaaaaag ggtcgtcgtt tagcaccatt actgacaggt ggagggcaag
    10141 aaagaaatca gcgaagtggg acagaaaatg ttccaggtat tgtggctatg gttaaagctt
    10201 tacgtttatc attagagata aaggaacaaa aacctgagca gacacaacgc ttaaaagtag
    10261 ctctaatcaa tgcgttgaag acgtatgaga aagtgacgat tttttcagaa ggaactgact
    10321 atgcaccgca tattttatgt tttgctctaa aaggaattcg cggcgaagtt ctcgtccatg
    10381 ccttagaaga aaaacaaata tttgtttcta ctactagtgc ctgctcctca cgtaaacata
    10441 tggcaagcag tacattgcac gcaatgcatg taccaaatga tctggctaca tctgctgttc
    10501 gaatcagctt agatgaaaac aattcgcttg cagaagtcga gcaatttatg attattttca
    10561 agcagcttta taagaaattt tctgcaatca attaaaagag gagtgttctt gtgaaataca
    10621 gtgaaattat ggtccgttat ggggaacttt cgacaaaagg aaagaatcga aaaagcttta
    10681 tcatgcaact agcacaaaat gtcagacaag ctttgaaaga ctttccagaa atcaaaatcc
    10741 atgctgatcg ggaccggatg catcttttat tgaatggcgc agacgggcag ctagttattc
    10801 caaaactagc aaaaatattt gggatccaaa atttctctcc aagtattcgt gtagaaaaag
    10861 atgtcgaagt tttgaaacaa gctgttcaag acatcatgaa agaaatttat actgggaaag
    10921 aaacatttaa aattatcgct aaaagaagcg atcaccaatt tgaattgaca agtaatgaat
    10981 tgaatcaaac attaggtaat gctgtttttg atattttccc gcatatccaa gttcaaatgc
    11041 gtcaaccaga tattccatta cgtgtagaga ttcggcgtga tggcgcttat ctatcgtatg
    11101 aaacgatcaa gggagctgga ggattgcccg taggaacaag cggaaaagga atgcttatgc
    11161 tttctggggg gattgattct cctgtagcag ggtactttgc catgaagcgg ggagtggaaa
    11221 ttgaagctgt tcattttgct agtccgccgt atacaagtga acaagcactg caaaaagcaa
    11281 aagatctaac agccaaacta gcaccttatg tgggaacgat ccattttatc gaagtgccat
    11341 ttaccgagat tcaagaagaa atcaaaaagt cagtgccaca aggatattgg atgacaatta
    11401 ctcgtcgaat gatgttgcgt ttgaccgatg aaattcgccg tattcgtcat ggtttagtca
    11461 ttttgaatgg tgaatcatta ggtcaagtgg cttcacaaac tttgcaaagt atggtggcta
    11521 tcaatgaagt aacgaatacg ccaatcattc ggcctgtagc gacaatggat aaacttgaaa
    11581 tcattgaagt agcagaacag atcgatacat ttaatctttc tatccagcct tttgaagatt
    11641 gctgtacgat ttttgctcct ccacagccaa aaacacgccc tcaactggaa aaggtccatc
    11701 aatatgaaga gcgtttagca atagatgaaa tgatccaacg agcactagct ggcttgaaga
    11761 tggaagagat acaagcagaa tcgtctaatg aaaaaactga agaattttca gaattgctat
    11821 aagaggttat gaaaatagtg ttttgaccag agtattagag aaacaaaacg aaaaaatagc
    11881 tccatgtatt ttaccgtttt tgaactaatg tcgaagatta gctgattgaa taccatttat
    11941 caagaagtta tggaaaagcc ggttcttttt tactaactga aaaataaaat tttatttaga
    12001 gatcaagcta cagttgataa atgatataaa aaagagaatt tcccatgaaa tagcgaggtt
    12061 gtgacagaag tgggcagctt caagaaataa gaaggaattt acgaaaattg cttttcaaat
    12121 ttttgtgaaa tttcggctta tttttcgaag aagttacttc tggaacaccg tgtatcagag
    12181 gttg
//
LOCUS       DAZBTF010000079        12133 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000079 DAZBTF010000000
VERSION     DAZBTF010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.079"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..610
                     /locus_tag="ACHGNS_002342"
     CDS             <1..610
                     /locus_tag="ACHGNS_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN6938265.1"
                     /translation="KRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWIQLIL
                     DEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSKITVF
                     PKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAYTIQN
                     ATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            723..1001
                     /locus_tag="ACHGNS_002343"
     CDS             723..1001
                     /locus_tag="ACHGNS_002343"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6938266.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            1119..1355
                     /gene="secG"
                     /locus_tag="ACHGNS_002344"
     CDS             1119..1355
                     /gene="secG"
                     /locus_tag="ACHGNS_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFN6938267.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            1458..2213
                     /locus_tag="ACHGNS_002345"
     CDS             1458..2213
                     /locus_tag="ACHGNS_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFN6938268.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            2216..4573
                     /gene="rnr"
                     /locus_tag="ACHGNS_002346"
     CDS             2216..4573
                     /gene="rnr"
                     /locus_tag="ACHGNS_002346"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFN6938269.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            4595..5059
                     /gene="smpB"
                     /locus_tag="ACHGNS_002347"
     CDS             4595..5059
                     /gene="smpB"
                     /locus_tag="ACHGNS_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFN6938270.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            5361..6080
                     /gene="atpB"
                     /locus_tag="ACHGNS_002348"
     CDS             5361..6080
                     /gene="atpB"
                     /locus_tag="ACHGNS_002348"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFN6938271.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            6133..6348
                     /gene="atpE"
                     /locus_tag="ACHGNS_002349"
     CDS             6133..6348
                     /gene="atpE"
                     /locus_tag="ACHGNS_002349"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFN6938272.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            6439..6963
                     /gene="atpF"
                     /locus_tag="ACHGNS_002350"
     CDS             6439..6963
                     /gene="atpF"
                     /locus_tag="ACHGNS_002350"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFN6938273.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            6950..7492
                     /locus_tag="ACHGNS_002351"
     CDS             6950..7492
                     /locus_tag="ACHGNS_002351"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFN6938274.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            7515..9071
                     /gene="atpA"
                     /locus_tag="ACHGNS_002352"
     CDS             7515..9071
                     /gene="atpA"
                     /locus_tag="ACHGNS_002352"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFN6938275.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            9085..9987
                     /locus_tag="ACHGNS_002353"
     CDS             9085..9987
                     /locus_tag="ACHGNS_002353"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFN6938276.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            10010..11416
                     /gene="atpD"
                     /locus_tag="ACHGNS_002354"
     CDS             10010..11416
                     /gene="atpD"
                     /locus_tag="ACHGNS_002354"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFN6938277.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            11430..11852
                     /locus_tag="ACHGNS_002355"
     CDS             11430..11852
                     /locus_tag="ACHGNS_002355"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFN6938278.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4035 a         2155 c         2677 g         3266 t
ORIGIN      
        1 taaaagatat gcagagaaag gattcaacgt tcttgtacct gatttacgtg cgcacggaga
       61 aagtgaagga gaaatcatcg gaatgggatg gctagatcgg ctagatttaa ttgcctggat
      121 ccaactcatc ttagatgaac agccgaatgc gtccattatt ttacacggcg gatcaatggg
      181 agcttctacg atcatgatgg caagtggtga aaaacttcca tcagcggtca aaggatttat
      241 tttagacagc ggatatgttt ctgtttatgc agaattccga tatatgttaa gtaaaatcac
      301 tgtctttcct aaaaaaatgg tcatgagata tgcgaatcat tatgcccaga aatacgccgg
      361 ttactcctta aagcaagcat cagctacgag acagcttggc agcaaccatc tgcctttatt
      421 agtcatccat ggtgaaagag atcattttgt ccctacagaa gcagcgtaca cgattcaaaa
      481 tgctacggca ggggataaag cactgctgct tgttccagaa gcagaacact tggaagcatc
      541 gatgaaagat ccgaaaacct attggacagt gattttttca tttattgaac aaagaataac
      601 gatggattaa ttttctcttc ttttttgtaa gtaattcgag gataaataat tgtttataga
      661 tctggagact gctaaaataa ggtaggaata aaaaaatatc agttatttga ggagtgcggc
      721 atatggaaat caaagaagaa aaaaatcgtc ttgctttatt caatgatgag caacaagaga
      781 ttggagaaat gacatggtca gatgcgggcc cagacatcat gatcattgac catacatttg
      841 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
      901 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
      961 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     1021 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     1081 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     1141 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     1201 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     1261 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     1321 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     1381 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     1441 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     1501 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     1561 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     1621 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     1681 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     1741 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     1801 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     1861 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     1921 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     1981 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     2041 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     2101 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     2161 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     2221 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     2281 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     2341 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     2401 agtgaaactt cctttaaaac caatcttgat cgaaggaaca tttcgagcaa atgaacgagg
     2461 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     2521 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     2581 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     2641 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     2701 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     2761 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     2821 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     2881 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     2941 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     3001 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     3061 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     3121 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     3181 tgtatatttg actgatcgag tgattccgat gatcccgcaa cgtttatcga atggtatctg
     3241 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     3301 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     3361 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     3421 tcagctcgta cctatgttcc aagaaatggg tgaattgcat cgaatccttg aaacgatgcg
     3481 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     3541 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     3601 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     3661 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     3721 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     3781 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     3841 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     3901 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     3961 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     4021 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     4081 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     4141 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     4201 aggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     4261 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacaaaaag tgcgcgtaaa
     4321 agtagtcaaa gctgatccag atgaacgagc agtggatttt gaactcgttt ccgcagaaga
     4381 ggtcactgcg ttggaacgtc caaaagcacg caatggaaaa tctggaaaac gagaaggaaa
     4441 acaagcaggc aaacgctcag aaaaaaatgg caagaagaac aaacgatata gtgatagtaa
     4501 gcaaacgttc ggtaagaaaa aagggaaaaa gccattttac aaagatgtca aaaagaagaa
     4561 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaactaat
     4621 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     4681 ggtcttgcaa ggaacggaga tcaagtcgat acgaaacagc cggatcaatc tgaaagatgg
     4741 atttattcgc gtccgcaacg gggaagcttt cttgcataat gttcatatca gtccttatga
     4801 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaagaa
     4861 acaaatcgtt cgtcttgaaa acgaattgaa aaacactgga atcactgttg ttcctttaaa
     4921 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcaaaag ggaaaaaatc
     4981 gtatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     5041 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     5101 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcac aggttttttc
     5161 ttagatcagg agcaccgtgc atggggaaaa attcgcttcg tttttgactg aaataaaaaa
     5221 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     5281 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     5341 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gtttcacatt ggaccaattt
     5401 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     5461 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     5521 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     5581 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacataggtt
     5641 tagtaacaaa aatcgtctta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     5701 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     5761 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     5821 ttccgatgaa actgattgaa gaatttacaa atttattaac acttgcttta cgtctttatg
     5881 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     5941 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     6001 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     6061 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     6121 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     6181 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     6241 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     6301 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     6361 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     6421 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     6481 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     6541 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     6601 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     6661 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     6721 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     6781 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     6841 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     6901 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     6961 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     7021 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     7081 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     7141 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     7201 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     7261 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     7321 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     7381 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     7441 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     7501 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     7561 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     7621 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     7681 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     7741 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
     7801 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
     7861 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
     7921 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
     7981 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
     8041 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
     8101 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
     8161 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
     8221 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
     8281 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
     8341 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
     8401 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
     8461 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
     8521 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
     8581 cggttgatgc cggactatct gtatctcgtg tagggggttc tgcgcaaatc aaagcaatga
     8641 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
     8701 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
     8761 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
     8821 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
     8881 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
     8941 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
     9001 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
     9061 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
     9121 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
     9181 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
     9241 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
     9301 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
     9361 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
     9421 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
     9481 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
     9541 tctgatcagc caagctttga tgaagtccgc aaaatcgtaa atatggcaac aactatgttc
     9601 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
     9661 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
     9721 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
     9781 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
     9841 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
     9901 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
     9961 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    10021 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    10081 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    10141 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    10201 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    10261 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    10321 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    10381 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    10441 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    10501 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    10561 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    10621 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    10681 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    10741 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    10801 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    10861 attacaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    10921 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    10981 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    11041 ggcttcttct tcaagtgctt tagcacctga aatcgtgggg gaagaacatt ataaagtagc
    11101 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    11161 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    11221 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    11281 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    11341 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    11401 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    11461 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    11521 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    11581 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    11641 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    11701 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    11761 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    11821 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    11881 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    11941 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    12001 tggaacaccg tatatcagag gttgtaaaat cagcgttttg tcctgagtat tagagaaaca
    12061 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    12121 tgaacaccgt tta
//
LOCUS       DAZBTF010000080        11783 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000080 DAZBTF010000000
VERSION     DAZBTF010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.080"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            510..1073
                     /locus_tag="ACHGNS_002356"
     CDS             510..1073
                     /locus_tag="ACHGNS_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938279.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(1134..1499)
                     /locus_tag="ACHGNS_002357"
     CDS             complement(1134..1499)
                     /locus_tag="ACHGNS_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFN6938280.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1575..1820)
                     /locus_tag="ACHGNS_002358"
     CDS             complement(1575..1820)
                     /locus_tag="ACHGNS_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN6938281.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1890..2144)
                     /locus_tag="ACHGNS_002359"
     CDS             complement(1890..2144)
                     /locus_tag="ACHGNS_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938282.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(2308..2631)
                     /locus_tag="ACHGNS_002360"
     CDS             complement(2308..2631)
                     /locus_tag="ACHGNS_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938283.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2818..2891
                     /locus_tag="ACHGNS_002361"
     tRNA            2818..2891
                     /locus_tag="ACHGNS_002361"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2852..2854,aa:Ile,seq:cat)
     gene            complement(3276..3773)
                     /locus_tag="ACHGNS_002362"
     CDS             complement(3276..3773)
                     /locus_tag="ACHGNS_002362"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFN6938284.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(3813..4622)
                     /locus_tag="ACHGNS_002363"
     CDS             complement(3813..4622)
                     /locus_tag="ACHGNS_002363"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN6938285.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            4956..5813
                     /locus_tag="ACHGNS_002364"
     CDS             4956..5813
                     /locus_tag="ACHGNS_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFN6938286.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            5806..6708
                     /locus_tag="ACHGNS_002365"
     CDS             5806..6708
                     /locus_tag="ACHGNS_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFN6938287.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            6741..7091
                     /locus_tag="ACHGNS_002366"
     CDS             6741..7091
                     /locus_tag="ACHGNS_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFN6938288.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            7140..8063
                     /locus_tag="ACHGNS_002367"
     CDS             7140..8063
                     /locus_tag="ACHGNS_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFN6938289.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            8141..9034
                     /locus_tag="ACHGNS_002368"
     CDS             8141..9034
                     /locus_tag="ACHGNS_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN6938290.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            9072..9635
                     /gene="amaP"
                     /locus_tag="ACHGNS_002369"
     CDS             9072..9635
                     /gene="amaP"
                     /locus_tag="ACHGNS_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFN6938291.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            9649..9837
                     /locus_tag="ACHGNS_002370"
     CDS             9649..9837
                     /locus_tag="ACHGNS_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFN6938292.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            9850..10752
                     /locus_tag="ACHGNS_002371"
     CDS             9850..10752
                     /locus_tag="ACHGNS_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFN6938293.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            10784..11026
                     /locus_tag="ACHGNS_002372"
     CDS             10784..11026
                     /locus_tag="ACHGNS_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFN6938294.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(11282..11653)
                     /locus_tag="ACHGNS_002373"
     CDS             complement(11282..11653)
                     /locus_tag="ACHGNS_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938295.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         4176 a         1985 c         2232 g         3390 t
ORIGIN      
        1 attcattggt cgttgaatgg gctgaatagt agtgtataaa acaattttgt ggagcgtgaa
       61 gcttttatgg tagaggaaaa tacgaataaa gtgatgttga taaaaagatt cctatcttga
      121 atatgaatcg agatgtcttg aaaatgaatg agtttgacca atcattatcc tatcgaaatt
      181 tacagtttcg ttcgattggt ctgtcaatta atgaaaaagt aaaaaagaat atgttctagg
      241 aagagggcgg cacatctaaa tttttaagta acggacagtt caaatatcaa gcattgaaag
      301 aggctatcct actgtttgaa ggaatacgaa tatataaaaa cgacaaagag gaaaaagtag
      361 ttcattaaat cccttcaagt gatgaaagaa tgagatctaa taaaacctga tctttcatct
      421 gtttttttaa taacgtaata atttttttat tttttgtgct ttcgccgtta ttgtagagtg
      481 atttagcatt cttattttta aaaataactg tgttatatat taaaaaaggc ataatagtta
      541 taagcttttt taaagaatat ttagaaatta ttaattttcg tctgaaaaca aaaaaagaaa
      601 gcgttattat tttagtctat tttcaggggg ggaaccttat gataaaaaaa ctatttttac
      661 tatcaatatt aacgctagca atggcaggtt gttctgactc tggcttagca actgaactga
      721 tcacaaaatc aactgaagcc acgacagagg agttaaaatg tggaaacgat cacagtgact
      781 acagaggctt aaatcgacga tgcatgggaa tattaggcag aagcaattgt aagcatcgat
      841 gtagtaatcg aagaaggtgt agaagaagca acagaaaaat caaaagaaag atcaaaagaa
      901 gcaatctgtg ctgtgcatcg tatacagacg tcgaagatgc ggtactttta aaccacgtca
      961 ggagatgttc atttatgtct agggaagttg gcgatgaacc aaaactagag tacgctttta
     1021 gttttgcacg tgaggatgaa gataacccta aaacttacga cattttagct tgatctaaga
     1081 tggttaaagc tttttaatga agtaagagga gatagaaggc ttttctagtt atattaggta
     1141 agataaattc ctttttgctc caaatttgct aaacattctg ctagataaac ttcatcgtgt
     1201 tccggataaa tcggcacgtt gcgctctaca gtaggtaaat caaaaaatgc ttcgcatgat
     1261 ttgccacgat acataggatt tttccactct ggtgctgggc tgtaaccacg tttttccatt
     1321 tcatccatga caagctgatg atattggaat aatctataag gagaatagtg aaaaacataa
     1381 tccacagtag catgtttctt tccccagcca tttcctctta atgcacagca ttcccgatgt
     1441 tgtcctaaaa gctgttgacg aggaagcttt gaaatcaatt tttcatgcca taatcgcata
     1501 gaaatgtctc ctttatatat agattgttaa ccttagacgt taaatgaaaa aaccagcacc
     1561 aaggctggaa aaaatcactt cagtttgctt gctgtttctg agcgaccaat taggtaatca
     1621 acggaaatat cgtaaaagtc agcgatatag attaattcaa tgatgcttgg aatttcttct
     1681 ccttcttcgt acttacgata tttgaataaa ggaatactta acgcgctaga aacagctact
     1741 tgagataaag atcggccagt tcttaagaaa ttcagacgac aacgtaaagt atccagtaaa
     1801 atagcttctt cgatactcat ttaatcacct ttcattatag tagaatagtt ataatgacta
     1861 tagaaagcta cacatgcaat caatagtact caatgttttt tatttaaacg tatcaaataa
     1921 agaatgacga tcaataaaga caaaaccagt cctgataatt cttctataaa aacactatcg
     1981 ggttcaaagt agttgtaatt aaaaactttc cacataaagt agatagccgg aagagtcaaa
     2041 atagctaaat aaaaccgttc tttgttccaa ttcataagat aaacaatact tgcgaccagt
     2101 gcgaccgcag gcacccaaaa caaaactctc accaacgtaa tcatgtaacg gactcctcaa
     2161 cgattcatat aataaaagat aacatgttat tgcatacatc acaaatgaaa tgctaggaaa
     2221 ataaaagaag aatagatcaa tgaaaaatga aaaagcagaa cagttttctg ctgataagca
     2281 ggaactgttc cgctgaatgt gtcttatcta tcgagaaaaa atcaatggat tgatatctaa
     2341 gtaattcaat acatataaaa agaggacgat cgcattcact aaaaaggaaa gaatgatgat
     2401 cccatagcga atggaaacat atttaacaaa tacttttgtt tgaatacggg catatctttt
     2461 tcctttatag ctgagcatca tgacaagaag caaaagggta atcacacctt gaaaaatcat
     2521 tccaataaac agaattcctg aggtttgata atttaattgg agaaaataag ctgctgccgc
     2581 aactaaatcg aataaaatcg caataaatgg caaaataatc actcctaaca ttgagaataa
     2641 ataaaagaat aacatttttt ctaaaataag caaataaaaa ttaaagtaac aaaatagtta
     2701 tgacacaagt aaaagaaata agaataacaa tattatttta cttatatttt gaaacttgtg
     2761 agagtttgtc attcgtgcta tagttgaaaa gtaacaaaaa taaattttat ttatgatgta
     2821 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
     2881 tacaaggtac ataagaggtt gtgaaaaagc tgccctgcat caagtaataa gaacagccaa
     2941 gtaaaaacag cttcttatgt ttttacttgg ctgttcttat tactgcagat gcaagctttt
     3001 gaataccgtt taaggaagag gttgtgaaat cagcgttttg acctgagtat tagcttaaca
     3061 aacggaaaaa tagttcctca tattttgccg tttgttaagc taataccgag ggtcagctga
     3121 ttgaacacca tttaagaaag aggtcgtgag cctgccgttt cactctgtgt cataaaaaag
     3181 ccaatctact ttgatagaaa aagcacgtag gactaaaaca gttctgcgtg ctttttttat
     3241 tgtgtgttaa atatcgtaaa tgaagataga tatttttatg attcgacgat agagacgttg
     3301 aaatctttca acagctctaa agcggattct ccgtgttttc tactggagac taacattgga
     3361 tcaacaaaaa agtctgcttc aggaaaagca ctctgcaggc ctatcagatt agagagtaca
     3421 cacaagtttg tttcaatccc gactacttcg atctcttttt gttcttcttt gctttcaaat
     3481 aaagtttcct ggatttcagc taaagtttca ggtggaatcg taaagtaata ctttttaatc
     3541 actcgttcat tttcttgtgg agaaagaaga gggatcaatt gcaaatcttc tcgttcttct
     3601 tctttgtcct tcagttcaac tggtatatcc cgtgtgaaga gaatatattc attattttgg
     3661 cgtgctttag ctaatctttg agcaatacgt tctgccaatt gatctgactc tggaatataa
     3721 aattcgctgt ttggatcaag aatatgattt tgcatatcaa taataactaa cattttcttt
     3781 cctcctacat tcatatcgtc attttattct ttttaataga agttttttaa aatataagca
     3841 acaccagctt cttcattcgt cagtgttact tggtttgcaa tttcttttac ttgtcgctgt
     3901 gcattcccca tcgcaatact caacccgctg taattcaaca tagcaatgtc gttttcttga
     3961 tcgccaaaag ccatgatgtc actcgattcg attccaaggt aatccgccag aaaaacaagt
     4021 gaatgagctt tagtgatacc tgtaggcgtg atttctaaga aatgcggatc agagaatact
     4081 gattggatac catctgcttg cagatctctt tgtagcttct gtagtttttc ttcactctta
     4141 tctgttaatg ctaatttaag atagctaaga gaaggatctt ccaacatatt taaaatttga
     4201 tcaaaagaat ggttctgtaa attgaaatgt tcataataag ggtcatcttt gactagattt
     4261 gattcatcga atgtcagctg atcatcttgg gtttctcggc tagcaatgat ggatattttc
     4321 gcttcttgag catagaagaa ggcttttctc agtgtatcgc tatctatttt tgaacgtctg
     4381 agaactttcg ccatttttcc atccttcatt tcagcaatca gtgaaccatt ggaagaaatc
     4441 aaatagccac cgattcctaa ttcggaaaaa atatctttag cagaaatgct gttccttccg
     4501 gtagcgacgg ttacgattcc gccattttca tcaaatttcc gaagtgcttg gatattttct
     4561 tctggtatct ttccatttcg atcaagcaag gtaccgtcta aatcaatcgc tgctagtttc
     4621 attttatctt cctactttct ttttacatat ctattttcct gttaccttaa gattaaatga
     4681 gaatttaaaa aaatgaaact aacttgcttt gtcattatga attttttcaa aaaacattct
     4741 taagaaaaag gtggctatat atagtagttt ttggtcaata cctttgaata aaaagccatg
     4801 aaaaacgaat agttagcgct taccgatgcg ttttgtgata aaaaaatttt aaaatattca
     4861 atgaaaacaa agcatatatt ttgctttaaa accaaatgag gaatattcta gatgtagaac
     4921 aggttagaaa aagattagaa aaaggagtga ttttgatgga gaagagatac ggcgtagttc
     4981 ctgtggtaac tgcttcagac gaaaattatg ctccttactt gagcgtgatg atcgcaactg
     5041 cattagaaaa ttgcaataaa acaagacgca taaaatttta tgtgattgac gatggtctat
     5101 cagaatatag taaagaagga ttggaagaaa cggtaaataa atattcaagt aatgcaagta
     5161 tccaattcct gacagtggaa aaagatatct acgaagactt cctagtcagt gaccatatca
     5221 caactaccgc ttacttaaga atttctttac caaacttatt agctaaagag gattataaga
     5281 aagtccttta cttagattca gatgtgttag tactagacga tatcgtaaaa ctatatgatg
     5341 agccgttaaa tggaaaaaca atcggcgcta ttatcgatcc aggccaagtg aaagctttgg
     5401 aaagattagg aatcgattcc gatgacttgt acttcaattc aggagtgatg gtgattgaca
     5461 ttgatcaatg gaataaaaaa gaaattacag aaaaaactat ccattacctc agtgaaaatg
     5521 gcgatcgaat catctatcac gaccaagatg ctttgaatgc tgtactttat gaagattggg
     5581 aacaacttca tccaaaatgg aacatgcaga cttcgctgat ttttgaacgt catccagcac
     5641 caaatgaaaa atatgaacgt cagtataaag aaggaaatga aaaaccttcg atcgttcact
     5701 ttacaggaca tgataaacct tggaatacgt tgaaagatca tccgtatacc aatttatact
     5761 taaaaaaatt agcacacagt actctaacga aagtaggcga gatcaatgaa taaaaaagaa
     5821 atagcagtag tcgcaagttg taacacaaaa ttcgtaccac atttagctgc attatttgtc
     5881 tcagtattag ataattgcaa cccgtctaaa tttgttcggt tctatgtgat tgacgatgat
     5941 atcgattttg aaagtaaaca gcttttacgc ttctcagtga aaaacgcacg aatgaactca
     6001 gatgtggaat tcttgaaaat caataaagaa ttctttacaa atgtggtaat cagcgatcgt
     6061 attccagaaa cagcttatta tcgtattgcg attccggaat tgttccgcgg cacagaagtt
     6121 gaacgtatct tatatatgga ttgcgacatg atcgctttac aagatatctc taaattatgg
     6181 cgtcttgact tcggtgattc aatcgtagca gctgttgaag acgctgggtt ccatcaacga
     6241 ctggaaaaaa tgaaaatttc agctaaatca atgcgttatt tcaactcagg tttgatgctg
     6301 atcaatgtga aaaaatggtt agatgaaaat attacgcaaa aagtattaga tttcatcgaa
     6361 cataatccag aaaaattacg attccatgac caagatgcat taaatgctat cttacatgac
     6421 cgttggttac cacttcatcc gcgttggaat gcacaaggct atatcatggc aaaagcaaaa
     6481 aaacatccaa cagcagcagg tgaacgtgaa tacgaagaaa ctcgcaacaa tccttacatt
     6541 atccattttt caggacatgt gaaaccgtgg agcaaggatt ttgaagggcc aacgaagaaa
     6601 tattacgaaa aatatgcagg gatgacggct ttccgttgtg tagctaaatt ccctaaatat
     6661 ccaaaatatg caaaagttca accaaaaaca gatgtagttg ttaaataata gaagaccaca
     6721 actgaagagg gggagtagga atggaaatta caatgtctaa aaatgcagta gagacgttaa
     6781 ttgaaaaggt tggggaaaat acaaaaatcg cactcgcgct tatcaatgat tccgatccat
     6841 ttctaaggga caaaggtgct tttgcaaaag gcagcttttt tcaaatcatt ccttttgttt
     6901 cggaatttgg agaatatgcg actaaaatcg agcatccatt attggacatc tatacatcta
     6961 aattagagca aaattatttc ggtaaacgat tgaacatgga cttcaacaaa cagcttgaca
     7021 gcttttcatt agaaaatgaa atcgcagtac tcgattataa tatcaaatta aagaactgct
     7081 tcttcagcta atagtttcga gaaagtccaa aaaggaggta tacattgaga tactactttg
     7141 tggatgaaga aaagaatgta atagaaacag atgaaaaaaa atataattgg ctcgtacttg
     7201 atagtgcaaa gcccgaagag attgagcaag caatcgaaga attcgagctt cctgatgata
     7261 ttttcgtagg aaccgaatat ccggaagaag tttctcggat ggagcgcctt tatgagacga
     7321 aactagagca tccattttct ttggtagtga ttaatctaga tcagtcagat aatcgtatcg
     7381 aaaaaaaatt aacgccaata tcttttgtac tttccgataa tctcttgatt acttgtatcg
     7441 atagaaaaac cgaatttgtg gatcggctgc tgacaaagca tggaaatcat ttaaactcgt
     7501 ttgaaaagat catcacgtat acattattag atatttacac ccattacgtg aaagaactaa
     7561 gagagatgaa aaagcgaatt gacgctttgg ataaagcagc tcgtaaaaca acggaaaatg
     7621 aagaactgta taaacaggca gatcttgaaa gagacatcgt atatatcgat cataccttaa
     7681 gagatcaacg ggaaacaatg gatcaattat ggaattctga aactttcatc aaacgtttag
     7741 acgatgaaca gctactctat gatgtccatt tgagacagcg acaaacagaa aaaatgatcg
     7801 agatctatcg agatttatta gagagtattg gtgatctatt caacgggatg atggacaata
     7861 atctaaatca tatcatgaaa tatctagatt cagctgcatt aatcatttcc gttccggcgt
     7921 tgttcgcagg gatttgggga atgaatacag gaggattacc gggtaaaagt tctgctctag
     7981 gctttatact cgtagtcttt ggctctttga tagcagcagt agttttaggt taccatttat
     8041 accggaaaga ttatacaaat taaagaatct atttaaaaca ggtatcaaac aaagaaattt
     8101 gttttggcaa tacaatttaa aaactaggag gaatacagca atgacagaac caaaattaat
     8161 cgacccgcga aacttgtacc atacggagaa atttcctaaa caagatcagg atacaccagc
     8221 tttacagcag aatatgattc ctgttcctga ttgtggagaa gaatcctatg aaggcaataa
     8281 tcaactagaa aaccgccgtg tactaatcac tggtggagat tcagggatcg gtcgtgcagc
     8341 agcgattgca tttgctagag aaggggcaga tatcgctctt caattcttcc caggagaaga
     8401 aaaagatgca gaggaagtag caaaatacgt gaaagatgca ggacgtaaga ttgtcttact
     8461 accttttgat cttcgtgacg aacaagcacc taaagaaatc atagataaag ccgtgagtga
     8521 acttggcggg ctagatacga tggtactaaa cgcaggacag caaatttctt gtcagtctat
     8581 tgctgagtta ccacttgagc aagtcaaaga cacatttatg ataaacatca tcgctatgtt
     8641 tgctttagtg aaagaagctg ttccgcatat cccagcagga ggagcaatcg taacaacaac
     8701 ttctgttcaa gcattcaatc caagcgaaca tttattagat tacgcagcaa caaaagcttc
     8761 aatcgctaac tttacgatcg gtttagcaaa acagcttgca cctaaaggaa tccgtgtaaa
     8821 tggtgtagca cctggtccaa tttggacacc attacaacta gacgaaggac aaccttctga
     8881 ttcgatccct gaatttggtc agcaaagttt actagaacgt gcaggtcagc caggagaatt
     8941 agcaccagtt tatgttttct tagcatcgaa taaagcaagt tatgttacag cccaaattta
     9001 cggagtaact ggtggagaag cgatcaactt ataagaagtt gatcgtttca cccctatgag
     9061 gaggtgcgaa aatgaataaa ggtgtaaaaa cgatcggagt gatcggaaca ttacttttgc
     9121 tatctgtgct tttgtttgcg atactattaa gtgcaaccta tccattgcct tttcagctag
     9181 agaagtttcg attcttttcc atcactaatt catacgtgca acaatatgta ttttggcttg
     9241 ccgttgcatt tgctattttg acaattatca cgttacttgt tttcctgttt tatccaaaaa
     9301 caatcggaaa ctttctactg aaacatggtg atggggagtt gacgttggat aaaaaagcaa
     9361 ttgaaggaat ggttcgttct cacttgcatg aagatgaatt catccattca ccaaaagtaa
     9421 atgtcaaagc aacgaaaaac aagatcgccg tcaatgtaaa aggggagttg aaacgaactt
     9481 cagcactcat tggtaaaaca ggaacattga tgaaagaaat cgaaaatgaa gtacaacaca
     9541 tattgggaac aaaagagcca gtgaaagtat cggtgaaata cacaggcttt gaagaaccag
     9601 ctaaacgaag caatgaccgc aaacgagtag aatagggggg gaccaaaaat gaaagaacta
     9661 ttttcgacat acaagctccc tatcatcttt ggggtacttg gattgatatt agccgtttta
     9721 tggatcaccg tgggcttttt caagacctta ttgatcttga tcatggcagc gatcggcgtc
     9781 gctatcggtt tttatctgaa acaaacgaaa atcttagatc actattttaa atcttaggag
     9841 gaaacatcca tgaataacaa taacaaaaat aataacaata aaatgaacaa ccaaaaacca
     9901 gtgtctgcac atccagaccc aacgacacca aaccaatcaa atggtgtgaa cgatgaaaag
     9961 aacaaaacag caccagtatc tgcacatcca gatccagtag tccctaacgt tcaacaagga
    10021 gtagaagaga agaaacatga tgcagaaaag catgcacctc atcatccagg aactccagaa
    10081 tctaaagttc cagaacaaca tgatgcaatc gatccttcta aacctgtgga agagcaaaaa
    10141 catccttctg accatgcgaa agcacaagaa aaacttagtt ctgaccacac aaacggacaa
    10201 ggaaaacttg gttctgatca tacaaataac caaggaaagc atgattctga acaggaagat
    10261 tcaatgcgcg gtgagctgaa ttatgaagat aaagttgtgc aaaaaatcat cggaatcgca
    10321 attgaacaag tagacggttt attatctgca aacggtggtt tcttctctaa tgtggcagga
    10381 aaacttgtga atacagataa tgtaacggct gggatcgaaa cagaagtcgg taaaaaacaa
    10441 gtagcagttg atatggatgt cattgtggaa tatggcaaag atatcgaaaa aatctttgaa
    10501 gaaatgcaag aagtaatcgg caaagaagta aaaaatatga cacatttaga agtgatcgaa
    10561 gtcaacgcta acgtagtgga catcaaaaca aaagaagaat ttgaaaaaga tcaagaaacg
    10621 gtgcaagaca aagtaactga cgcagcgaaa aaaaccggtg aatttgcttc gaatcaaaca
    10681 gataaagtaa aatcagcagc tggaagcggc gcacaaaaag taaaagaaaa tacagaacca
    10741 cgggttcagt aataaggaag taaaacatta ggaggaacga aacatgggat ttatttggtc
    10801 acttatcgtc ggaggaatta tcggagcaat tgcaggagca atcacgaata aaggttcttc
    10861 gatggggatt attgcaaatg tcgtagcagg tttagttgga tcagcacttg gtcaagcatt
    10921 attaggttct tggggaccaa gtattgcagg aatggcaatc gtgccatcat tgattggtgc
    10981 catcatttta gtactaatcg tatcattctt tgctcgaaaa ctgtaaaaag acttttaaaa
    11041 agaggtcgtg aaaaagctgt tcttcatcaa gtaataagaa cagccaaaca aaaatagctt
    11101 ctcatatttt tcatttggtt gggcatatta tcgaagatgc aagcttttga acactgttta
    11161 tacggaataa gaggctgtga caagactttt gtcacagcct cttatttatg cgggcaaatt
    11221 cccaattaga tttttaaaag tttttcctta aatgtgtctt tattgataca gagaagcttc
    11281 tttaataaaa caatgcctcc ctaaattttc ttgagatagg aaaggtgaac atacaaaata
    11341 atacgaccac tttgaaaacc cagatgatcc acaaatggat cgtatgatac tgatgaagta
    11401 aaaagcaatg gatcagaaac tcaaagagat tgaaaaagac aaggaagaaa taatatctct
    11461 tttttactct tgtctgtttt agccaataat agctcgtaaa gtaatctaga caaacaaata
    11521 gaagtaaaca aagtttcgta agagcagcaa tcgtatcatg ttgctctgct agacgattga
    11581 cgagcaggaa aaatgttggt aatggaatat agatcacgag taatgaaaga aaaaagtaaa
    11641 caataaatgg catactattc ctcctaattg tgtgagttcg ccctcatttt aaaataacga
    11701 tatttgtttg tgaagctttt tcgtgtcgta acaaatgtta tctaatgtat ttttaaataa
    11761 ataatcatta ttatcaaaat aat
//
LOCUS       DAZBTF010000081        11755 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000081 DAZBTF010000000
VERSION     DAZBTF010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.081"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..177
                     /locus_tag="ACHGNS_002374"
     CDS             <1..177
                     /locus_tag="ACHGNS_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6938296.1"
                     /translation="YLLGMALAYSVGSLIKVNVSLDLFTILLAVGVSSVIGLVFSVMP
                     ASEAAKKDLIDILR"
     gene            578..1183
                     /gene="fic"
                     /locus_tag="ACHGNS_002375"
     CDS             578..1183
                     /gene="fic"
                     /locus_tag="ACHGNS_002375"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFN6938297.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            1199..1771
                     /locus_tag="ACHGNS_002376"
     CDS             1199..1771
                     /locus_tag="ACHGNS_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN6938298.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2023..2280)
                     /locus_tag="ACHGNS_002377"
     CDS             complement(2023..2280)
                     /locus_tag="ACHGNS_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938299.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(2406..3221)
                     /locus_tag="ACHGNS_002378"
     CDS             complement(2406..3221)
                     /locus_tag="ACHGNS_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6938300.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            3738..5063
                     /locus_tag="ACHGNS_002379"
     CDS             3738..5063
                     /locus_tag="ACHGNS_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFN6938301.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            5056..5406
                     /locus_tag="ACHGNS_002380"
     CDS             5056..5406
                     /locus_tag="ACHGNS_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938302.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            5403..5573
                     /locus_tag="ACHGNS_002381"
     CDS             5403..5573
                     /locus_tag="ACHGNS_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938303.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHKYEYYIGY"
     gene            complement(6477..6752)
                     /gene="prgO"
                     /locus_tag="ACHGNS_002382"
     CDS             complement(6477..6752)
                     /gene="prgO"
                     /locus_tag="ACHGNS_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFN6938304.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(6724..7677)
                     /locus_tag="ACHGNS_002383"
     CDS             complement(6724..7677)
                     /locus_tag="ACHGNS_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN6938305.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            8289..9782
                     /locus_tag="ACHGNS_002384"
     CDS             8289..9782
                     /locus_tag="ACHGNS_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFN6938306.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            9917..10213
                     /locus_tag="ACHGNS_002385"
     CDS             9917..10213
                     /locus_tag="ACHGNS_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFN6938307.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            10314..11165
                     /locus_tag="ACHGNS_002386"
     CDS             10314..11165
                     /locus_tag="ACHGNS_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938308.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            11186..11398
                     /locus_tag="ACHGNS_002387"
     CDS             11186..11398
                     /locus_tag="ACHGNS_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938309.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         4041 a         1811 c         2179 g         3724 t
ORIGIN      
        1 tatttattag gaatggctct tgcttatagt gttggttctt tgataaaggt aaacgtgtca
       61 cttgatctat ttactatttt actagcggtt ggggtatctt cggttattgg actggtcttt
      121 tctgtaatgc ccgcttcaga ggctgcaaaa aaagatttaa ttgatattct tagataaaat
      181 cagaatgaca agaatattta cgttctagat tctaaaataa aacacaaaga gaccaaccag
      241 gcaccctatt gtttctttgt agtatagaga tattctttgg cttgtaagag atcaatagag
      301 ttaggtgacc ggagttcgaa ataaaggaaa tttttgattg tataggagaa ttctttatcc
      361 ctaatattta gaattttgag tatgccatgt tgatgtttta gattctctat aaatatagag
      421 gtgtatcaaa ggtcaactag actgtgcaaa atagagacac ttgaaaaatc tatcgggggt
      481 cagaaactta atagtttttg acacataaac aaaatcgcga tataatgggg ttgagagtgt
      541 ttcataaact tatgtcataa agttaaggag atttcgcatg attttagaga ataaattggg
      601 attaaccaat caagttgagt tggctaaggt tgaagaaaaa ctcagtaaac aaaaagcgaa
      661 agaattgtac gattgtggaa aaataaatga aatcgaagtt ggtacattta aaggtttatc
      721 tgaaattcat gaatttctat tttcggatat ttacgatttt gccggaaaaa tccgatcggt
      781 caatattgca aaaggaaatt ttcgatttgc accagttatg tatttagaac attcgttaca
      841 gcatattgat cagatgccac aaacttcttt tgaagagatt atcaagaaat atgtggagat
      901 gaatatcgct cacccatttc gagaaggaaa tggccgaagt actagaattt ggctggatct
      961 cattttaaag gaagaattgc aaaaagttgt agattggaac ttaatcgata aagcagacta
     1021 tctttctgca atggagcgaa gtcccataaa cgatttggaa atacgctatc taatttccaa
     1081 tgctctgact gataaaattg acgatcgcga gttatatatg aaaggaatcg atgtcagcta
     1141 tttctatgaa gggtattctg agtatacgat agatgatttg taaaagtagg tggaccaaat
     1201 gacgaaatat ggatatgcac gtgtctcaac cgcttctcaa agtacaaaag aacaaattca
     1261 acagttgctt gagaacggcg tagagaagaa gaatattttt tcagaaaaat ttacgggtac
     1321 aaaacgggat cggccaaaat tcaatcagct gttggctggt ctgaagcctg gagatgaaat
     1381 agttgttacg aagcttgatc gatttgctcg taacacgaga gaagcattag agatcattga
     1441 acctttactc gatgataacg taacgatcaa ggttttgaat ctaggaacga ttgaaaatac
     1501 gtccatgggg cgaatggtga cacggacgtt gttgagtgta gcagaaatgg aacgtgatat
     1561 gattgtggaa cgtacccaag aaggaaaaat gtttgcaaga aagaataatc ctaatttcag
     1621 agaaggacgt ccgaaagcaa caatcacacc gaagaagagg cacgcatatg aactgcttac
     1681 ttcaggtaaa tcgtataagg aagttgaagc tataaccggt ttcagtcgca gtacgctttt
     1741 tagaataaag aagaaaattg aagaaagtta aaaaagaagt ctcagcgaac cagacaggta
     1801 aatacttaat gcgttctaga tatgagtcta ttagcatgtt gtgaaatata atattttttt
     1861 atttacttga tttctgctca aaaaatgtgt atggtaactg taaaaggtaa agtaattaaa
     1921 ggtatgctag ccacgcttac tatattagga taccatcata tatttataat gaacgatttg
     1981 ttcgtctacc aaacaacaca tatcgttcag cgtaagtcac gtttaggaac gtggcttttt
     2041 tagtgttatt atcgataaat aatctattga ctctattgat tttatctcca tatccctacg
     2101 gtgttgattg tctttagatc tttcatttaa gaaccaaata actatcataa aaatcagcac
     2161 tgctgctaat ataataaaca gcattcctat tgtgatatta cctattgatt ttaccataac
     2221 tacgattaaa gtaataatgg cattcaagtc tcctttgttc tctatatata aattagccat
     2281 aaatcatctt cctttcaaat taagatactt gtttgatacc aataaatagt aagcgtggct
     2341 agcatacctt tttctgtaat agcaagtgtt ctaatagtcc aaacttaatg atggaccgat
     2401 gctagttata gtacagtaat gttttctttc ttatctagat ttttagtaag ctctatcagc
     2461 aaatctaaca aatctgagtc cttttcagaa actaaattaa atctagtgtt tacaacaaag
     2521 ttttcatgtt ctatattgtt tagattattt gcggaaagga gggcttctag atcttgacat
     2581 tcaaatctat atgcatcgtc ataaatctta ctagaaggag tgcttaattc gaaattactt
     2641 attttctcat cttggaccaa gataaattta tagatagtat ctttttgtgt ttttctttta
     2701 aaaacacaac agagtgtgtt ataaggaacg ttataatttt catcagcctc attttttctc
     2761 cattcaaaca aatgataaac tgcaaccaag ttgccatcaa tttcatggac agtgctctcg
     2821 gtaacatcct ttaaagaact atcaagggct aatttatagt aactagagag aggaagtaaa
     2881 attgtcgatt tactgagatg gttatcgtct atacgtgcaa tcatacctaa atcaactaag
     2941 gttttattcc aggtgtttat agttttagtt gtcactccta gagactctgc tatggtattg
     3001 accgaatgcc aggaatcccc gccttcattc tttgccttaa aacagtaata cagatataaa
     3061 ttaatagctt tgttattcgg taaatatttt tcaagttctg ttggtagaat aaagtaagaa
     3121 tccttaatgt tttttctgaa ctgtctccag tctctgtagt tttgtttata aagttgtgaa
     3181 acggtttttc cacctttaga aagtcctgct atcttaacca atcttattgc tcctttcttg
     3241 aatctgtcat gatcatacag tattttttac ttgtagtcca gcaccttaat atattatcct
     3301 atgtaattaa tttcgtacta gatagtaatt attttttatt taattctcta gtcacttaca
     3361 aataagttat ataaactacg attaataaat tattacaaaa actattaaga aaacttctac
     3421 atacaactat tacaaaacta ttaagaaact gaatactcct attttttgtt tttaaggaaa
     3481 gttttttctt ttttgaattt cttatttttt tctaatataa ctaagcaaaa ctggataata
     3541 cgacacttat tttcaaattt tcacaagact aatagtaaat atccatttaa taaagtgata
     3601 caatgtgttg attttacaaa tcaacagaag aagtagggaa ataacttacc tatgcttctt
     3661 tttttgtaca tttaatcgat tcttctttac aaaaacgaac gtatgtttgt ataatgtgga
     3721 agaatggagt gttatttatg atggattatt caaaagagcc agtaaatgat tactttttga
     3781 tcgatatgaa aagcttttac gcaagtgtcg aatgtataga aaggaattta gatccattaa
     3841 cgactgaatt agtcgtcatg agtcgagcgg ataatacagg atcagggttg attttagctt
     3901 cttcaccagc tgcaaagaag aagtacggta tttctaatgt cagtcgacca cgagatctac
     3961 cgcaaccttt tcctaaaacg ttacatgtcg ttccaccacg catgaatctc tatattaagc
     4021 gaaatatgca ggtaaacaat attttcagaa ggtatgtagc agacgaagat ttattgattt
     4081 actcgatcga tgaatcgatc ttaaaagtga ccagatcaat gaatcttttt acgactgaag
     4141 gaacaagaag tcaacggaga aagaagctgg ctcaaatgat ccaggagaat attaaagaag
     4201 agctaggatt gcttgctaca gtaggtgtcg gagataatcc cttattagct aaacttgctt
     4261 tagacaatga agcaaaacat aatgatggat tccttgctga atggacgtat caaaatgttc
     4321 ctgataaagt atggaatatt cctgaaatga cagacctttg gggtattgga tctcgaatga
     4381 aaaaaagact caaccaaatt ggtatcatga gtattaaaga cttagctaat tggaatccat
     4441 acactattaa gagccattta ggtgtgattg gcttacagct gtattttcat gcgaacggga
     4501 tcgatcgaac agatatagcg atcccaccag aaccgacaaa ggaaaaatca tatggtaatt
     4561 cacaagtgtt accacgagat tataccagga gaaatgaaat cgaactggtg gtaaaagaaa
     4621 tggcggaaca agtagctatt cgtatcaggc aacataactg taaaacaggt tgtgtccatt
     4681 tatacattgg cacgtcgatc ttagaaacga gaccaggttt ttcacaccag atgaagatcc
     4741 ctataacaga taatacaaag gaattgcaga attactgtct tttcttgttt gataagtatt
     4801 atgaaggaca agaagtaagg cacgttggaa taacctatag caagcttgtg tacactgatt
     4861 cgcttcagct ggatttattt agtgatccac aaaagcaaat tgacgaagaa aatttagata
     4921 aaattattga caagattcgg caaaaatatg gttttacgtc gattgtacat gcttcttcta
     4981 tgttagaagg cgctcgtagt attactagaa gtacattggt tggtggccat gcaggcggcg
     5041 caggagggct taaaaatgat tgattataat gatcgtggac gtttaaagtg ggcaggtttt
     5101 tatttatctg atcatactga aaaaattgat gccgacaatg aacaacgagc caatcaaaat
     5161 ttggcaaaag aacaaatgac gactgaagag gttgcggaag tattgaacgc tgcagtcata
     5221 aaaaatagat cagtatgtat tcaaaaagaa gaacgaaacg ctgaaggata ctatccagaa
     5281 gatattgtta gcaagatctt aggtagtgat gagctgggga tctacgttgg aaaaaacaaa
     5341 gttcactacg acgaaatacg tcatatcgaa ttttaccagg ataataaatg gggtcgatta
     5401 gaatgaacat tatccagcaa tatgagctaa agtatataac ctttgatcag ctatcagaag
     5461 agatttgggg ttatggccag cgtttgataa atgaggttgg tgttgaacgc ttctctttct
     5521 atgtagaagc tgcagcagga taccataaat acgaatacta tattggctat taaagtgcaa
     5581 taaaattata tttcttttat gttttacatt ttgtatcaat tctgataaaa ttacctatag
     5641 gtaattaaaa aatataaaaa aactagttac aaccttagga cgttgtaact agcttgaagc
     5701 atactagagc taactaaaat agcactagaa agcaatgtta aacccgtcca acaaacttga
     5761 tttaacattg agaccagctt attcagctaa atatagttga ataagcttct tttgttttaa
     5821 aaaaacaaaa ggtgtactac gaataacgca agtaaaagaa atactattac aaccagtagg
     5881 ctacaagtaa gtattactaa acattgcgta gttagaacct ttgctctatt gtctaaagcc
     5941 acaaaattag agatcagctt tatgatgcta agagcgagct gcatccatgc ggacataggt
     6001 tcttttgaca ctattaattc tcctttttag gataagggaa attgctagta gttttttcat
     6061 tagcaccatc ccccttcatt tgattgagtc tagtgctgct gtgatagttt gctgtttaat
     6121 atgcttacat tcttattata acataaatga aacaaacaca tggttagagg tcgtatcaat
     6181 caaaatagtg tctcacaaac gatttattac ttactcctgc tgactaacgg actgttcttc
     6241 agtaactgta actatatgtt tgttaaaacc accattacct gtagataaaa accaatatat
     6301 ttactatact cactatatta aatatattta gtatagtaaa aactagaaac aataaaattt
     6361 gtgaatgttg tataccccca aacaatacac atattttgaa ggattaggat taataaagct
     6421 aaaaatatag taagtatact aaatatattt actatatcta gtatacttac tatattttaa
     6481 tcttttgatc gtctattata tataatccgt tcctcgtcgt ttaaagtatc aacataggcg
     6541 tccattgcaa gtttgactat ttcatacata ggaatatctt ttgcataact aagatcccga
     6601 atggttttta gtacgggagg atctacttga actggttttc tttctttcgc agtgtacttt
     6661 cgttccccta actcgacatt aatatcatct ttaattgaaa tatcttcagc accaattttc
     6721 ttcttatctt tgttcgttgg taataatgcc atttagttta atctcctttc caagaggtag
     6781 gatatgattg gccattgagt cagcatattt aggtgtataa tcaaatcctt caatatcacc
     6841 atgttgttca tagtattcaa ttctttcttc taattctgta aagatgtctg caaaaatagc
     6901 ccaccatctt cgatctacat aatcatttaa ttggatacca ttctcaccat acatttctaa
     6961 ccgatccgag cctttaacaa tgcttttaaa aatgtttgat tcaccatact tatcaattgt
     7021 tttttgatag tttgcgtgtt gagaggaacg tcgcactgag aaaagaacag gcaaaattcc
     7081 aatgatttga agatgagact cttcaaaatt cgtgacaatc ggaactaaaa ctttattaat
     7141 aaaatcttct gttccttcca ttgcgtaccg ttttaattct tgcattgcaa taatataatc
     7201 tgcagcagtt aagaatgcac gaaccacatt atctgttgat ggtgggcagt cgataaagat
     7261 ataatcgaaa ttttttctca attcttctaa aggtttaatg aaggccaatg atctcttacg
     7321 acgatcagga tatttttcgg acaagtattc attaagttct tcagatccag gagagccagc
     7381 cataatatat aaatgtggac ttacttgagt aatacctgaa acccagtccc cttttttgat
     7441 tgctgcagac atggatcgat caaagctagt aatttgatac gttgatccaa tgttggaagt
     7501 agtatttctt tgttggtcat aatcaattag caacacgtct ttttcaaaaa gttgactaga
     7561 aacaatcgct aataaatcat tgtctgtagt cttaccaatg ccgccttttc tacctgtaag
     7621 cattacaact tttcctagtt tatctgatgt tttaaatgca gtccgtaagt tttccatagt
     7681 tccctccaat ttcctatact aaatatattt agtatacttc atatacttaa tatattcgat
     7741 atatctaata tactccaaat tgataagaat tcagacttta taaggaatat agtggatata
     7801 ctaaatatat ttgctatatt cagtatatca gatatattca aaatgtcaaa atatatttgt
     7861 gcgttttgcg ttgacaattt agagtgaaaa gaggtaaact taattctata gaaaccacag
     7921 ataattcact atttacaaag tagcaaaagc agtggtactc tttaaacaga aaaattacat
     7981 aaaaaaaaga gacggaaata attgatttta gcagaatcaa ttatttctga atcacatggt
     8041 taggacatgt gttcacgctc gtctcaagaa tatgtaagta gctaccaact acttatatta
     8101 aaagttgatc ggataccttt tccgattgcc tttattctac caaaaaaagg cgagcaactc
     8161 aacagaagaa cgtttattta gtgtacttaa attaaatatt cttaaacact gaagagagaa
     8221 cacatactat ggctgagatt aaagctaaac tgtgttctct ttttgtttga gaaaggacgt
     8281 tacaatcgat gttgagcgaa atttattcag ctattcttaa aaacggctta cgtacataca
     8341 aatatcgaaa cagcaccatg aaacctgcag gtcatattga ttccagtagg agtggtgcta
     8401 tctttgcgta ccgtagcaag caattaatga atgttgggcg tggtatggtc attacttcgg
     8461 aagaagctat tttagagaac gaaaagaaac taacgcattg gacacctaat acctatcgct
     8521 ttggtactta tgcggatgat tatcgaacag tggttaaagg tcattctgag aaaaacttaa
     8581 gtcaaatcaa cacatttgta gttgatatcg attcaaaaga gaaccatcaa ggcgaaatta
     8641 ttttagcctg tttagatcaa gtgggctaca tgccaacgct aattttagaa tcagatcatg
     8701 gttatcaagt ttattttgta ctaaagacac caggctatgt cacaaagaaa agcaatttca
     8761 aggtgatcga tgtagcaaaa aaaatttcaa aaacaattcg tctacagttg gctgaaaaat
     8821 tgtccggagt ggatctagga tgtaaccact tcggaattgc tcgattccca caagaaaata
     8881 atattgtttt taaagaaatg aactatcagt attcttttgc tgactggatt caatggtcga
     8941 tgaagcttga atcagatcaa aaaacaaaag aagatcgaga acgaaaacta attgtctttc
     9001 ctgagaaaaa agaaattcgt caagtagatg aaccgtggtt cgatatgtta cttcataaag
     9061 caaacatcgt cggcggagaa ggacgtcttg gtcgaaataa tgtgattttc acgctttctt
     9121 tagcttatta ctcttcaggt tatgcattgg atacttgcga atacaatatg ttcacattca
     9181 acgaacggtt acaggaaccg ctatctgaag cagaattagt aaagattgta cgtagtgctt
     9241 attctggtaa ttatcaagca gcaagcagag aattcattat tgaattgtgc cagcaatggg
     9301 tttcaccaac cttaaaaaat aatgatctgt tcattcaacg acgtggctgg tggaaattta
     9361 aaaagccacg agatcaaaga aaatatagtc ataaaaatga atggaaagaa gatctattgg
     9421 cttatctaag cgaaaaaagt cttgctagtg agccttattt gaacctgtca aaaaaagaat
     9481 tggcttcaca actaacgatg cccaagcgaa cacttgataa gatcttaaat gagttacgag
     9541 aggagaataa agtcttctac aaggtaacac gaggaagaaa tggcggaatg atcattgctt
     9601 cagtcaaaac gttgttcgcg agggttattc aactaagtaa agaaaagaga ctagattatt
     9661 caacaaggat cacagaggta tttggactca acgagagctt agtagaagag acatttagac
     9721 aattatcaac ccccttgaac cctcttaaag aagtgaatct ttttgaagta gatacaggtt
     9781 gaaaaataaa aggtgcaaat tgccttatac aatatatcta tgatacttct gtaggtgttg
     9841 ctccctttgc ctagctacgt gtaggtggta tacttataac tgcgtatgca gtcttatgta
     9901 tcatggagga agttgtatgt cgttaaaaaa ttatgtctat tcacatcctg ttaatgtgtt
     9961 tatcattggt cgtttgggat tatctgtgga agtgttctgt gagctttatg gttttaagca
    10021 ggggactttg tcttcttggg ttaccaggga aaaaacagtt gcttctttgc cgattgagtt
    10081 tcttcatggg ttgtccttag cttctgggga gactatggat caggtatatg attgtttgtg
    10141 tgtgttagag caggagtata ttgagtataa aatagctaac gagttaagaa aaaggaagaa
    10201 gtatatccag tagtagtttt tatttactta aaaaattggt tcattgagca acctatcgct
    10261 gctaaagtag caaaggaaaa cgttaaagga aagaatttag ggaagaaatc gaaatgaaat
    10321 cggacgactg gattgaacac acctggcttc ctctgaaaag aaatagcaaa cgaaagaaat
    10381 tttgggtatt gcaattatgg ccattaggaa aactttgtag ttacggggca cgacttattc
    10441 ttgatttaac cgtttactcg attggagtat ttggaacggc ttattacttg ataaatgatc
    10501 ccaataggat tgcagcatca gacattttta cagtgctaat gacgttgatt tttgtcatgc
    10561 tattgtctat ttttactcaa ttagtcatgc aatttaccag gcaaaaatta acagcaggaa
    10621 aacttttcaa aagcctattc gatttaattg ttgtaaatgc aattctagcg actttaataa
    10681 aacaaattga acaatctgga agaacaacaa aaggagtgaa ggatttttta ttgacgttta
    10741 ttcactttgt aaatggacac atgtatttgt ttgaagtggg tattattctc ttttttgtcg
    10801 ggtatgtatc cacgaaactt attaaacgtt caattgaaaa agaaattgaa attgacctga
    10861 ttccgacact tcttggttat gagtacaatg ttgattttgg aagatatgac gaattgatca
    10921 atacggatat cgttcacacg agtatccatg aatctactgt taagggcatc agatctattt
    10981 taaaaaatgg ccaggaaatg aaagcaatag atcgaagaaa agagatctac aatttttatg
    11041 aagtcgaagc aagcctacga tatggcatcg gaaaattgtg gttagatgga agtaatattc
    11101 gtcaaaacaa accatacatt ttttggaaca gtagtgattt agaacttcca atgaaagata
    11161 aataattatg gagaatcgtt tgaaaatgat tcctctatac aggattgtgc ttatagttgg
    11221 catggagtta aaaaccttaa agaaggagca gttattcaaa gtgaaagaac gtcataatca
    11281 gttactatgt agtaaaacgg gtacgatgaa cattgaatac cgaacagaat atttagaatt
    11341 aacgaatcaa actgattttt tgttgattcg aaagcaacaa gagcaagaaa aaaattaatg
    11401 gctacttata gccaattgat tgatgaatgg gaacaattca aggaatctaa aattccagaa
    11461 cgtctctaaa agcctttatt tgtaaagagc ataaggaaac gtgggaacac tttttaaaaa
    11521 aagtgttcca ataggctgtg cctattacgt ttctcaaaaa taaatgtggg aacaaaatat
    11581 ttaaaaatag gatagtgaaa ggatagtgaa ggatagtaaa aggagggtag taggatagca
    11641 aaaaggatag gctccttttc gctatccttt ttaaattgga ggaattaata tggaagtaag
    11701 aataagaaat gtggatgaga aaatcgtaag agaaattgat cgaagatgtg acgaa
//
LOCUS       DAZBTF010000082        11608 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000082 DAZBTF010000000
VERSION     DAZBTF010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.082"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACHGNS_002388"
     CDS             6..305
                     /locus_tag="ACHGNS_002388"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN6938310.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACHGNS_002389"
     CDS             378..1739
                     /locus_tag="ACHGNS_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFN6938311.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACHGNS_002390"
     CDS             1761..2021
                     /locus_tag="ACHGNS_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938312.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACHGNS_002391"
     CDS             2035..2787
                     /locus_tag="ACHGNS_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFN6938313.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGKKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACHGNS_002392"
     CDS             complement(2881..3486)
                     /locus_tag="ACHGNS_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN6938314.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACHGNS_002393"
     CDS             complement(3539..4513)
                     /locus_tag="ACHGNS_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFN6938315.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACHGNS_002394"
     CDS             complement(4891..5610)
                     /locus_tag="ACHGNS_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN6938316.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHGNS_002395"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHGNS_002395"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFN6938317.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACHGNS_002396"
     CDS             7161..8558
                     /locus_tag="ACHGNS_002396"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN6938318.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACHGNS_002397"
     CDS             8639..9079
                     /locus_tag="ACHGNS_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFN6938319.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACHGNS_002398"
     CDS             9227..9553
                     /locus_tag="ACHGNS_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFN6938320.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACHGNS_002399"
     CDS             9554..10303
                     /locus_tag="ACHGNS_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFN6938321.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACHGNS_002400"
     CDS             complement(10383..10838)
                     /locus_tag="ACHGNS_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFN6938322.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="ACHGNS_002401"
     CDS             complement(11265..11540)
                     /locus_tag="ACHGNS_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938323.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
BASE COUNT         3666 a         2014 c         2358 g         3570 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtaaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaag
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaag
//
LOCUS       DAZBTF010000083        11293 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000083 DAZBTF010000000
VERSION     DAZBTF010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.083"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            12..84
                     /locus_tag="ACHGNS_002402"
     tRNA            12..84
                     /locus_tag="ACHGNS_002402"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="ACHGNS_002403"
     tRNA            88..160
                     /locus_tag="ACHGNS_002403"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="ACHGNS_002404"
     tRNA            168..249
                     /locus_tag="ACHGNS_002404"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="ACHGNS_002405"
     tRNA            267..339
                     /locus_tag="ACHGNS_002405"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="ACHGNS_002406"
     tRNA            351..422
                     /locus_tag="ACHGNS_002406"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="ACHGNS_002407"
     tRNA            439..524
                     /locus_tag="ACHGNS_002407"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="ACHGNS_002408"
     tRNA            535..608
                     /locus_tag="ACHGNS_002408"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="ACHGNS_002409"
     tRNA            618..691
                     /locus_tag="ACHGNS_002409"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="ACHGNS_002410"
     tRNA            711..783
                     /locus_tag="ACHGNS_002410"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="ACHGNS_002411"
     tRNA            811..884
                     /locus_tag="ACHGNS_002411"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="ACHGNS_002412"
     tRNA            898..971
                     /locus_tag="ACHGNS_002412"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="ACHGNS_002413"
     tRNA            995..1084
                     /locus_tag="ACHGNS_002413"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="ACHGNS_002414"
     tRNA            1097..1170
                     /locus_tag="ACHGNS_002414"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="ACHGNS_002415"
     tRNA            1189..1264
                     /locus_tag="ACHGNS_002415"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="ACHGNS_002416"
     tRNA            1266..1336
                     /locus_tag="ACHGNS_002416"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="ACHGNS_002417"
     tRNA            1350..1423
                     /locus_tag="ACHGNS_002417"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="ACHGNS_002418"
     tRNA            1448..1535
                     /locus_tag="ACHGNS_002418"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="ACHGNS_002419"
     tRNA            1557..1628
                     /locus_tag="ACHGNS_002419"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="ACHGNS_002420"
     tRNA            1651..1740
                     /locus_tag="ACHGNS_002420"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="ACHGNS_002421"
     tRNA            1753..1826
                     /locus_tag="ACHGNS_002421"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="ACHGNS_002422"
     tRNA            1845..1917
                     /locus_tag="ACHGNS_002422"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="ACHGNS_002423"
     tRNA            1921..1993
                     /locus_tag="ACHGNS_002423"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="ACHGNS_002424"
     tRNA            1998..2068
                     /locus_tag="ACHGNS_002424"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="ACHGNS_002425"
     tRNA            2081..2154
                     /locus_tag="ACHGNS_002425"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="ACHGNS_002426"
     tRNA            2166..2237
                     /locus_tag="ACHGNS_002426"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /gene="metK"
                     /locus_tag="ACHGNS_002427"
     CDS             2503..3696
                     /gene="metK"
                     /locus_tag="ACHGNS_002427"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFN6938324.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="ACHGNS_002428"
     CDS             3826..5298
                     /locus_tag="ACHGNS_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFN6938325.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="ACHGNS_002429"
     CDS             5381..6169
                     /locus_tag="ACHGNS_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN6938326.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="ACHGNS_002430"
     CDS             complement(6236..6904)
                     /locus_tag="ACHGNS_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFN6938327.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="ACHGNS_002431"
     CDS             7019..8731
                     /locus_tag="ACHGNS_002431"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6938328.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10506
                     /locus_tag="ACHGNS_002432"
     CDS             8734..10506
                     /locus_tag="ACHGNS_002432"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6938329.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(10588..>11293)
                     /locus_tag="ACHGNS_002433"
     CDS             complement(10588..>11293)
                     /locus_tag="ACHGNS_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN6938330.1"
                     /translation="MIFKFANRRYGLKYNPVNGVSLARKPKTNEEIKKEKDGYLSKEE
                     VQMIASKQKSSHQQSRYSLITKFLFLTGLRYGELISLTESDYDGNKITVSGTYDYELK
                     IKTTTKNTGSYRTIELSSNAKEIIDQLIEENKLIKNNKDKYIFISKNGNPISIQAYNQ
                     SLRAVSKELNLDKKVSSHMLRHSHISLLTELGIPLKAIMDRVGHEDSKTTLKIYTHTT
                     KNMQNQLVEKLGKIEI"
BASE COUNT         3250 a         2022 c         2554 g         3467 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 agttaaatta aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt
    10561 ttttataagg ggcagaaaag gggcaattta aatttctatt tttcctagtt tttctactag
    10621 ttgattttgc atatttttag ttgtatgagt ataaatcttt aatgttgttt ttgaatcttc
    10681 atgtcctaca cgatccataa tggcttttaa tgggattcct aattctgtaa gtagagaaat
    10741 gtgggaatgc cttaacatgt gagaagaaac ttttttatct aggtttaatt ctttggatac
    10801 tgctcttaaa gattgattat atgcttgaat agatatcggg ttaccatttt tagagatgaa
    10861 aatgtattta tctttattat tcttaattaa tttgttttct tcaattaatt gatctatgat
    10921 ttctttagca ttacttgata attcaatggt tctataagaa cctgtatttt tagttgttgt
    10981 tttaattttt aactcgtaat cataagtacc agaaaccgta attttattac catcataatc
    11041 agattcagtc aaagatatca attcaccata acgtagtcca gttaaaaaca aaaatttagt
    11101 aattaaagaa taacgtgact gttgatgtga acttttttgt ttactagcaa tcatttgaac
    11161 ttcttctttt gataaataac catctttttc tttttttatt tcttcgtttg tcttaggttt
    11221 tcttgctaaa gaaaccccat ttacgggatt atattttaat ccatatcgac ggttggcgaa
    11281 tttaaaaatc att
//
LOCUS       DAZBTF010000084        11159 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000084 DAZBTF010000000
VERSION     DAZBTF010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.084"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1167
                     /locus_tag="ACHGNS_002434"
     CDS             <1..1167
                     /locus_tag="ACHGNS_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938331.1"
                     /translation="NGIFTSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIII
                     PEFDSRNILLALVRYVNWEGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIR
                     GIEIAIGRKAFPLGLKEEDKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFN
                     SRNYTNENMELLRKDFEVVYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQF
                     VRTLWADGQVFLPERIYLLSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKS
                     LSLINSEQAGRQEIEIFVVSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDE
                     CLCAKKRVVLCDAASYQNGLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            1388..3151
                     /locus_tag="ACHGNS_002435"
     CDS             1388..3151
                     /locus_tag="ACHGNS_002435"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFN6938332.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            3235..3693
                     /locus_tag="ACHGNS_002436"
     CDS             3235..3693
                     /locus_tag="ACHGNS_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFN6938333.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            3802..4485
                     /locus_tag="ACHGNS_002437"
     CDS             3802..4485
                     /locus_tag="ACHGNS_002437"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFN6938334.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            4632..5246
                     /locus_tag="ACHGNS_002438"
     CDS             4632..5246
                     /locus_tag="ACHGNS_002438"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFN6938335.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            5262..5468
                     /locus_tag="ACHGNS_002439"
     CDS             5262..5468
                     /locus_tag="ACHGNS_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938336.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     regulatory      5693..5859
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            6007..6564
                     /locus_tag="ACHGNS_002440"
                     /pseudo
     CDS             6007..6564
                     /locus_tag="ACHGNS_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            6622..8661
                     /locus_tag="ACHGNS_002441"
     CDS             6622..8661
                     /locus_tag="ACHGNS_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFN6938337.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDQYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGEKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKDDDDSATKRASELLQYLNQNYQ"
     gene            complement(8770..9921)
                     /locus_tag="ACHGNS_002442"
     CDS             complement(8770..9921)
                     /locus_tag="ACHGNS_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFN6938338.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVFLLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(10075..10605)
                     /locus_tag="ACHGNS_002443"
     CDS             complement(10075..10605)
                     /locus_tag="ACHGNS_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFN6938339.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(10704..11054)
                     /locus_tag="ACHGNS_002444"
     CDS             complement(10704..11054)
                     /locus_tag="ACHGNS_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFN6938340.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
BASE COUNT         4023 a         1894 c         2177 g         3065 t
ORIGIN      
        1 aatggtatat tcacgagtac agaactggct gatcttgaat ttatttcttt gagcaaagta
       61 tatacaatca aaaaaatcgt attggatttt tttaaagaaa atgattattt gaaaaataaa
      121 gaaatcatta tcccagaatt tgactcgcgg aacatcttgc tagctttagt acgttatgtc
      181 aattgggaag gctatgaaaa taaaaatccg tatgtagaag aagcttgtaa cgagttgatt
      241 gattatgtag agactcattt ttttaaaaga agatattcgg aagatgaaaa gtcctttatc
      301 atacgaggaa tagagatcgc aatcggaaga aaggcatttc cccttggact gaaagaagaa
      361 gataagaaaa gcgcagaaac gaagccgtta tttcaattag tctgtcaagg attggaagag
      421 caaaaaggaa ttctagactt acaagaagaa gatgtctatt atattttttc tttgttcaac
      481 agtagaaatt ataccaatga gaacatggag ctgttaagaa aagatttcga agttgtttat
      541 aaaaattttg ttctcaatga cctatcactg aatgaattga ttttacagat tattagtatc
      601 gctgaaatcg acgatgcaga aaattttatt ttcaagcaat cctttttaca atttgtccgt
      661 acgttgtggg cagatggaca agtattttta ccggaaagga tctatcttct atcagaaaaa
      721 gaacaacttc tgtacggaaa aattgtagaa atattggaag agtggaaaaa gaaaaatcaa
      781 atccaactaa gatggaatga aaatttcatt cgaaaattcg tcaaaagtct ttctttgatc
      841 aacagtgaac aagcggggag acaggaaata gaaatttttg ttgtttcaga aagttcagtc
      901 aaacaatttt tttatcgaac ccaatttcat ttgtatgcgg aagaaggcat cgctgaaata
      961 aatcctattt tatttcgttc attagaagaa ttgcctgatg aatgtctatg tgcgaaaaaa
     1021 agagtggttt tgtgcgatgc tgctagctat caaaatggat tagaaactga aaagacaaag
     1081 atctatccaa tctcattaaa aggtgcaaac atccatttgt atcatattgc tcaggaactt
     1141 cacctattaa aaaaacatat ttcttgatga gaccttaatt aggagacctt ctacaaagct
     1201 ttttaattgt ctgtagaagg tttttatttg agtcctgatt cgtgcgaaga tctacttata
     1261 taacaagctt tctctgaaat ttttttaaaa tgctattgac ttagagtcaa ctctaagtag
     1321 tagaatagat aaagtgtaac aaatctattt tcattaaaag aagatagtac ggaggagaga
     1381 atgtattatg agtacagtat tacatgtatt ggatgcaatc aatagccccg cagatgtcaa
     1441 aaaattatct gttgaggaaa tgaaacagtt ggcagaagag atgcgccaaa aaatcttgca
     1501 aaaggacagc gatgtaggtg ggcacgttgg tccgaatcta ggtgtagtag aattaacgat
     1561 tgcgtttcat tatgtatttg attcaccaaa ggataaaatc gtttgggatg tttcccatca
     1621 gtcttatggt cataagcttt taactggtcg aaaaaatggt tttgaaaaag gacattaccg
     1681 tgaagtgagc ggatattcca accaacatga aagtaaacat gactatttta cagtggggca
     1741 cacatctaca tctatcagct tagcaacagg tttagctaaa gcgagagatc tgaagaaaga
     1801 atcaggaaat gtggtagcat ttatcggtga cggctcccta agtggtggct tagcttttga
     1861 aggattgaac aatggtggtg ttttacaatc caatctaatc gtattagtaa acgataatga
     1921 gatgtctatt gatgagaatc atggcagttt atataaaaac ttagctgagc ttcgtcaaac
     1981 caacggtaaa agcagcaata acctatttaa agcaatggga tttgattatc gttacgtgga
     2041 taatggaaac gatctggaaa cggtgatccg tttatttgaa gaagtaaaag atattgatca
     2101 tccgattgta cttcacgtcc atacggaaaa aggtcgcggt tatcagccgg caatcgataa
     2161 taaaatgaaa tatcattggc atgtaccatt tgatctgtcc accggggagg caaagcaagt
     2221 aacttctgga gaaacattta atatgatcat catggattat ttggataaga aaatgcaaac
     2281 aagtacagct cctattgccg cagtcaatgc agcgatacca ggcgcctttg gactgaaaac
     2341 attccaagaa aaacatccag atcattattt tgacgtgggg atcgctgaac agcactctat
     2401 cactttcgct tctgctttgg cttctcaggg agtacgtcca gtcgtcttcc aatcaagtac
     2461 tttcttgcaa cgggcatatg atcaattatc acatgattta gcgatcaacg agaatccagt
     2521 gattatttta gtcggtggcg gaacgatctc agctagtgac cgaacacacc aaggaacatt
     2581 tgacattcct tacttatcaa gtatcccgaa catcaaatat cttgctccag caacaaaaga
     2641 ggatatgatc tctatgctag attgggcgtt cgaacaaaca gcgcagcctg tagcaatccg
     2701 aattcctaca catggtgtag aacatggtac agctatcgca aatgattata gtattccagc
     2761 ctatcaaaca gtaaataatg gaggaaaagt agcaatttta gctttaggcg gttttcttga
     2821 attaggaaaa gaaacagtaa aagcttttag aaaaaaagga atcgaagcaa cgttgatcaa
     2881 tccaggaacg atcacagaaa atgacgtaga aacattagat aaattaaaag aaacacacga
     2941 attagtcatc acattagaag atggtagttt gtcaggagga tttggcgaaa atatcagtcg
     3001 ttattatggt gattcttcaa tgaacgtatt aaattttggt gcagagaaag aattcacgga
     3061 tagcgttcct gtagggcagc tttatgaacg ttatcattta acaccaaaac aaatcgttga
     3121 agatagtctg cgtgtcttga atattcaata attcattatg ctcaccatcc agataattgt
     3181 ttatactagt atctggatgg ttttttcatt ataaaaagca aaggggtgga tgttatgtat
     3241 cagcaagtag aagaatatgt caaaaagttc caagaaaaat atcctagtta tcaatcagaa
     3301 aaattaggtt tcttttcatt tggggataca gaaaaaatgg cagacgagtt gagcgaacta
     3361 gttgtaaaaa agataaagac tggtacatca agtggttacg atctgtataa acaagagaat
     3421 gagcctttac caaaagttgg tcaattagac gtggtcctga atggaaaaaa tgaaccagtt
     3481 tgtatcatca aaaatataaa tgtgaccatc ttgcctttta gtgaagtcac gaaagagcat
     3541 gcttggaaag aaggagaagg ggatcggaca ttagcttatt ggcggaaagt ccatgctgag
     3601 ttttttaaac atgcttataa agaagcgttg aacatagaat ttacagaaga gaagctggtc
     3661 gtatacgaag aatttgaagt aattttcttt tagtaagaaa ggtctgctga aagaaatagc
     3721 tcattattgt ctcctgaata gaaaagggtt acatttttat atgaagctgt atttttatat
     3781 aacattactg gaggagatat tgtggataca ctggaacaaa tcaaaggcgg agtcgtcgtt
     3841 tcttgtcaag ctttggaaaa cgagccgcta cacagttctt ttattatggg gagaatggct
     3901 ttggctgcaa aagaaggcgg agcagttggt attcgagcaa atacgacaga agatatcaat
     3961 gaaatcaagc gaatcgttga tttaccggta atagggatta tcaaaagaga ttacgatggg
     4021 tcagaagtgt ttattactgc gacagaaaaa gaagtggaag aacttttgga gacgcaagca
     4081 gaaatcattg cactggatgc aacttctcgt aaacgtccga cagaaaaaac aactgctgaa
     4141 cttgtccaaa tgattcacga acacggtcgg ttagctatgg cagatatttc gacttttgag
     4201 gaagcgcttc aagcgcaaga agatggtttt gatcttttgt ctacgacttt agcaggctat
     4261 acagaatatt cacgaaagac gcagacacct gattttgatt tattgaatca aatcatagaa
     4321 caagtcgata tcccagtaat tatggaagga catactgact ctcctgaaca agtagagaaa
     4381 gcatatgaat taggtgcttt ttcagtcgta gttggatcta ttatcacccg tccgcaattg
     4441 atcactaaaa gatatgtcga agccgcaagt aaagcaaata actaaaaaac acgcacggca
     4501 cagattcttg acaaaaaaga aaaattgata gatagtttaa agcagattct aagaagatga
     4561 aaaaagcatt acttttcatt ttgtcttcaa gatctgcttt ttaacgaatt ttatagaaag
     4621 aaggcgaaac catgtttgaa cggagtcctt cagaattgac agaacgtgaa aattacaagt
     4681 tgctgattgg ttcagtcatc cctagaccag tagcagtagt aacgactaaa tcagcaaaag
     4741 atatagtgaa tatcgcaccc ttcagttact tcaatatcgt cagttcgaat cgacccatct
     4801 tatccttggc gatccagaga aaacaagatg cagtaaaaga cacagcaaag aatattcttg
     4861 agacaagaga agctgttatc catatccttg atgaggacaa cgtagaagaa gtaaataaaa
     4921 cagcagccaa tctgcctaat gaggaaagcg aactgtacct gactgacttg actttaactg
     4981 attcagtaat catttcagta ccagcattga cagaagcaaa agttcgtttt gaaacatcct
     5041 tatatgaaca tgtcgagata cggaataaaa ataaagtaac agctgattta tttttgctag
     5101 aagtacagaa atatcattta aatgaaacag tctatgatga taagacagga aggatcgatc
     5161 caagaaaatt gaatgctgta agccgtttgg caggaaatga ttatgcagca ataggagaaa
     5221 ttttcacgat tgcacgtccc gaataaaaag gagcactagt tatgttaaaa ataagctata
     5281 aaccatcgac agattcaaaa gaaatgaaaa aagaatatga aacagtgaat gattttctgc
     5341 aagggcagta cttagaagtt ccgccattgc aagaccattt tgtcgttact actgttactt
     5401 tagatggtaa ggaaatagaa atgccagatc aaacgattag cggattgttt aattacttta
     5461 acaaataaat atttattccg aaaagtgaaa aaacatataa aaaaacacat aaaactaaaa
     5521 aattatcaaa gaatcctggt gcaactgaaa gaatttgcaa cgggattctt tcaacttatt
     5581 tgttattttt ttctaaaaaa tattaaagat tgttaaattt cactatttca tttcaaattc
     5641 cattgcctaa tcattaggaa aagtctatca tgaactcgta aatagaatat agtaataacc
     5701 ttgttaggtg aggctcctat acaaacatag gctgttgccc agaaacgtcg aaagtcgcca
     5761 atgggtaaaa cagaaactgt tggattaagg ctttttccaa tagctaagaa tgttttcttt
     5821 acgttgtata gtgctaaaac tcggacgatg agatgatgct aggtcagaat tcttgttcga
     5881 aaaggtttct gaccttttct ttatattttt gtgactgact tatttgatga gacaaactcg
     5941 ctcctcacct gacaagtgga ttgctgtgtg aggaggagat gcggtgaaaa aagatcgatt
     6001 ggtggaggtc gctcggattc acttctggtt gcacaataca accagaaaac acaacaagca
     6061 aaactgatct ctatcatgag agattcatat gtcgatattc caggttacgg aatgaataag
     6121 ataaatgcag cttactctta tgggggagtt gatttattaa atcaaacatt aaaggaaaat
     6181 ttcaaatttg aagctcccta ttatgcaagt atcacatttc aagattttat cgattgcgtt
     6241 aatgaactgt ttcctggtgg agtcaagatc gatgcagaaa aatctctaga tttagatggc
     6301 gtatatataa acaaaggaga gcaagtgatg gatggtaata ctttactcca gtatgctcga
     6361 tttcgggaag acaaagaagg agacttcggg aggatcagaa ggcagcagca agtgatcaaa
     6421 gctatctctc agcagctgaa agacgtaact tcaatattca agctacccaa agcggttggg
     6481 aaattacttg gtagtatcca aacgaacctt ccagaaagtg tgcttctgga ttgtggtatg
     6541 gatttcctca aagacatatg ttagaataaa caggtataaa tagtgaaata aaggaatgac
     6601 aagcaagaga aggaggaaaa aatgaaaaga agtgacaagc acggcaaaaa tcgaacaggc
     6661 gcttatattg ccggcgcagt gatcttaata gcagctgcgg gtggcggtta tttttactac
     6721 cagcactatc aagaaaccca agcagtagaa gctggagaaa agacagttga gcaatttgtc
     6781 caagctttga acaagggaga ttataacaaa gctgcagaaa tgacatcaaa aaaggcggca
     6841 aataaaagtg cattatctga aaaagagatc ttagatcaat accagaatat atacggtgct
     6901 gctgatgtca aaggacttca gatatcgaat ctaaaagtag ataaaaaaga cgattctact
     6961 tacagttttt catataaagc aaagatgaat acctcattag gtgaattgaa agatctttct
     7021 tataaaggaa cattagacag aaatgatggt caaaccacga tcaactggca acccaacttg
     7081 gtttttccag aaatggaagg aaatgacaaa gtaagcctga ccacacaaga agcagcaaga
     7141 gggaatatta tagatcgaaa tggtgaacca ttagcaacaa caggcaaact aaaacaatta
     7201 ggagtcgttc caagtaaact tggagatgga ggcgaaaaaa cagccaatat caaagcgatt
     7261 gcttcctcct tcgacttaac agaagatgct atcaatcagg cgatttcaca aagctgggta
     7321 caacccgatt actttgtccc attgaaaatc attgatggag caacgccaga acttccagct
     7381 ggagctacca ttcaagaagt agacggcaga tattatcctt tgggtgaagc agctgctcag
     7441 ctgattggtt acgtgggaga tatcacagca gaagatattg ataagaatcc agaattaagc
     7501 agtaatggaa aaatcggtcg ctcaggtttg gagatggctt ttgataagga tcttcgtggg
     7561 acaacaggtg agaaattaag cattacagat gcagacggtg tagagaaaaa agttctgatc
     7621 gaacatgaag ttcaaaatgg caaagatatc aaattgacaa tcgatgcgaa ggcacaaaaa
     7681 acagcttttg acagtctagg aggaaaagct ggatcaactg ttgcgacaac gccaaaaacc
     7741 ggtgatcttc ttgcgcttgc tagctctcca agctatgatc caaacaaaat gacaaacggg
     7801 atctcacaag aagattacaa agcttatgaa gaaaatcctg aacatccatt catcagccga
     7861 tttgcgacag gttatgctcc tggatctacg tttaaaataa ttacagcagc aatcggtctc
     7921 gacaacggca ctatcgatcc aaatgaagtg ttgacgatca acgggcttaa atggcaaaaa
     7981 gacagttctt ggggatcttt taaaacaacg cgtgtcagta gtgatgtatc acaagtagac
     8041 ttaaaaactg ctttgatcta ttccgataat atatatgcgg cacaagaaac gttgaaaatg
     8101 ggtgagaaaa aatttcgtac aggcttagat aaattcattt ttggtgaaga ccttgatttg
     8161 ccaattagta tgaatccagc acaaatttct aatgaagata gctttaactc agatatcttg
     8221 ctagctgata ctggatatgg acagggcgaa cttctaatta ctcctatcca gcaagcagca
     8281 atgtattctg tttttaccaa caatggcaca cttgtctatc ctaaattgat tgcagataaa
     8341 gagacaaaag ataagaagaa tgtcatcagc gaaacagcag tacaaacgat cgtgccagat
     8401 ctgagagaag ttgtgcaaga tgtaaatggt acagcacatt ctctttctgc tttagggatt
     8461 ccgttggcag cgaaaactgg tacagcggaa atcaaagaaa aacaggatgt aaaggggcaa
     8521 gagaacagtt tcttgtttgc tttcaatctt gataaccaag gttatatgat ggtcagcatg
     8581 ttggaaaata aagatgatga tgattcagca accaaacgag catcggaact attgcaatac
     8641 ctcaaccaaa attatcaata aaaaattgct ttttaagtac agtaaaagga ctgtggcgtt
     8701 atcgtcacag tccttttttc aaataaacgg ttttcacgat gtctctttca caacttcttt
     8761 ttgatttact cacacttcat aaactttttt tgtaaaatat ttgagaacaa gtggagaaat
     8821 aagtgtactt aataaaatca cgataactaa aggtgaatag tactgtggtt cgatcaattg
     8881 actttgctgt ccaatttgta aaatgatcaa agccatttct ccacgcgaaa tcataccagc
     8941 accgaccatc aatgaactat gttgagaaaa accggcaatc tgagcaccaa aataacctcc
     9001 aagcaatttg gtcaaaatcg ctacaagcgt caatatagaa ataaagagta gttgttcact
     9061 aaagtcagaa aaatcaactt ctaatcctac actgacaaaa aatactggta taaatactgc
     9121 atatcctaaa gcctctacat tgtggtatac ttcttcttta acctttgtct gactgacagc
     9181 tatcccagca aaaaaagcac caatgacaga gctaagccca acaagatccg ctacgtaaga
     9241 cattcctaaa caaataacta gcgatacgat gataatagaa gaatttgcgt acactttttc
     9301 tgcaaatgaa agtaggaaag gcgcaatcca tttgactaat aagaaaataa aaacaaaata
     9361 aattagctgt tcaatcaaaa caagcggtaa agaagcattg ctagaagctt cccccgttag
     9421 aaaagatagg ctgaaactta gaactaaaac gactaaaatg tcatctacca cagaagcacc
     9481 caaaattgtt gatccttctt ttgtattcac tacgtttagt tcttttaata cttctactga
     9541 aatactgaca gaagttgccg caagaataat cccaaagaaa atcgcttcat ttcccgaaac
     9601 ttggaatgct ttgcctgtca accagccaaa aacaaccggg aagatgatcc caagcaaagc
     9661 aacgaacata ccaggtcgga aatatttttt caaaagagat aaatcacttt ccagaccagc
     9721 taaaaacatt aataagaaca caccaatctc cgaaaagtca tggactagaa tatcaggttt
     9781 gacccaattc aatccagctt ggcctagcaa aacaccaaca agtaattgtc caatcactgc
     9841 tgggatacct aatctccgcg agatatgtga gccaatcgtt gttgaaatca atatcaggca
     9901 taaaatacca ataaattcca ttctcatcat tcctttgtat aaaatatagc ccgaacttag
     9961 cataacatac attattgaaa aaataggaaa atatactcac aaaaaaacca acaatccaaa
    10021 agattttttt ggattgttgg tttttatacg gtctatttta ataactaaaa atgattaacg
    10081 tatagtcatt tttcttattc tgatgaaatt gataatctcc tggatgccca tgaaaatcaa
    10141 aataaagccg aatatttgga atagaagcat caaacttttg aatggattga acaataagac
    10201 gaatccgcca atcaacaaca gaatactact ataaacactc caacttttag cttttgtatt
    10261 catgctgaag aacaactgga acaagctgtt gatgataaca gctaatccaa gaagaatcgg
    10321 caggatagaa acgattgcag gtgcaaaagc atagacgatc agagctaaga tcaggaaaaa
    10381 aagaccactg aacagaccga tactccaaga acctgttttc cttttaaaac ggtaagcctc
    10441 aaaaagattc aacaacccaa gcaaaacaag ataggcggtg atcagataac cgataaaatg
    10501 aaaaacagca gaagggttta gcacaatcgc aatcccagaa ataatataaa cagcagctct
    10561 taataaggcg tatttttgaa tattttgttt aatcgtctca aacatataag aaaacctcct
    10621 ttgacttatt ttaacgtgaa ctagtcattt taccaaatta aatgttacaa aatgagcgaa
    10681 ggagaagaat atctctggaa aaatcaactt ctgaggatct tttccatttt tttgcctttt
    10741 gctagctcat cgatcatttt atctaaataa cgaatttctt tcatcaagtt atcctcgatg
    10801 tcttctactc gtacaccaca aacaacacct gtaatcaagg aacgtgaagg attggggaca
    10861 ggggcttccg caaaaaattg ttcgaaagtc acttgttttt gtagttgata ttctagttct
    10921 gtttgggaat aacccgtcag ccaacaaatg atttcatcta cttcctcttt tatacgatct
    10981 tttctctccg cttttgctac atacatagga taaacgcttg caaaacttgt cgtaaaaata
    11041 cgattttgcg ccataatcag tcacctttct ttttttcaaa ttatttacta cttatagtat
    11101 ataagaaaaa gagaagaaaa agcgattatt caaaaaaaca aaaaggattg tgacaaaat
//
LOCUS       DAZBTF010000085        11052 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000085 DAZBTF010000000
VERSION     DAZBTF010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.086"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(46..969)
                     /locus_tag="ACHGNS_002445"
     CDS             complement(46..969)
                     /locus_tag="ACHGNS_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938341.1"
                     /translation="MATNYAKKINFDVDKLYKSLIVVPKNKSEDGNYELEVLQFKIEQ
                     KSSERFMSNQLVKIKKWLPMIDQLVSNNKEAKNLCIFVLLLHGRVINLVNRKSTTKFA
                     NSFWFLKNINKTVEKNGVYKGDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETY
                     SQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRI
                     LKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLK
                     CRGLDFIGTLGWTETYYEKIYKEIIEQYFPK"
     gene            complement(1492..2496)
                     /locus_tag="ACHGNS_002446"
     CDS             complement(1492..2496)
                     /locus_tag="ACHGNS_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFN6938342.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDNGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK
                     "
     gene            complement(2567..2863)
                     /locus_tag="ACHGNS_002447"
     CDS             complement(2567..2863)
                     /locus_tag="ACHGNS_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938343.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            complement(2856..3149)
                     /locus_tag="ACHGNS_002448"
     CDS             complement(2856..3149)
                     /locus_tag="ACHGNS_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFN6938344.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            complement(3295..3846)
                     /locus_tag="ACHGNS_002449"
     CDS             complement(3295..3846)
                     /locus_tag="ACHGNS_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFN6938345.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            complement(4020..4319)
                     /locus_tag="ACHGNS_002450"
     CDS             complement(4020..4319)
                     /locus_tag="ACHGNS_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HFN6938346.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            complement(4346..4654)
                     /locus_tag="ACHGNS_002451"
     CDS             complement(4346..4654)
                     /locus_tag="ACHGNS_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFN6938347.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            complement(4771..5730)
                     /locus_tag="ACHGNS_002452"
     CDS             complement(4771..5730)
                     /locus_tag="ACHGNS_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFN6938348.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            5874..6650
                     /locus_tag="ACHGNS_002453"
     CDS             5874..6650
                     /locus_tag="ACHGNS_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN6938349.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            complement(6748..7395)
                     /locus_tag="ACHGNS_002454"
     CDS             complement(6748..7395)
                     /locus_tag="ACHGNS_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN6938350.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(7395..8225)
                     /locus_tag="ACHGNS_002455"
     CDS             complement(7395..8225)
                     /locus_tag="ACHGNS_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN6938351.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            complement(8225..8974)
                     /locus_tag="ACHGNS_002456"
     CDS             complement(8225..8974)
                     /locus_tag="ACHGNS_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFN6938352.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            complement(8988..9452)
                     /locus_tag="ACHGNS_002457"
     CDS             complement(8988..9452)
                     /locus_tag="ACHGNS_002457"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN6938353.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            complement(9502..9963)
                     /locus_tag="ACHGNS_002458"
     CDS             complement(9502..9963)
                     /locus_tag="ACHGNS_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN6938354.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            complement(9945..10919)
                     /locus_tag="ACHGNS_002459"
     CDS             complement(9945..10919)
                     /locus_tag="ACHGNS_002459"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFN6938355.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
BASE COUNT         3512 a         2047 c         1511 g         3982 t
ORIGIN      
        1 gaaatcacaa cttttttctt ttacgataaa tcaatcctta ttaaattatt taggaaaata
       61 ttgttcaatt atttctttat atattttctc atagtatgtt tctgtccatc ctaatgttcc
      121 tataaaatca agtcctctac attttaaagg ttcacttaaa ctattaatat agttaataat
      181 gttgtctatc cttttctctt tatcttcacc ttcaatctgg ggagcatata gtccaatttc
      241 ctcccaataa tatgaatctc tttcaattcc catgatggta gctttggcta atttcaaatc
      301 aaataacgat tttagaattc ttagtgtctc aataaagtct tctctcttca actctttaat
      361 ccattgtgaa ttttctggat tatctaaaat ttcaagttca tcttcattat aagtatattt
      421 caaatttgac ttgcttaact catagataaa tgaagtttct tgactcataa ttcttttaag
      481 tacttgacta taagtttcgt ttgactttgc ccatttcgct aattctacca ctgtattcac
      541 taataaatcg aaataatgat gttctcgttc aatagtctca gcattttgaa aatgaaaatc
      601 acctttataa actccattct tttccactgt tttattaata ttttttaaaa accagaatga
      661 gtttgcaaat ttagtcgtac tttttcgatt aactaagtta ataactcttc catgtaataa
      721 taagacaaaa atacataaat ttttagcttc cttattatta gatactaatt gatcaatcat
      781 agggagccat tttttgattt tcactaattg attgctcata aatctttcac tggacttttg
      841 ttcaatctta aattgcaaaa cttctaactc ataattccca tcttctgatt tatttttggg
      901 aacaactatc aaacttttat aaagtttatc cacatcaaaa tttatctttt ttgcataatt
      961 tgtagccatt aatacatctc cttattcata ataaatttca tatgacgctc ctctcaaaat
     1021 ttaccggtag actagaaaaa agaataaaag atacgtcaat tcacacttta ttcttaaaaa
     1081 aaaactggtc tctctaccgg taatatcgat tgagtccgcc tttatcgact ttatcctttt
     1141 tttatattaa attttttcac cggtagacag ggagagaaag aataaactaa aaagccctta
     1201 attcttaatg ttttcacctc cttaccgttc ctaccgctaa aatttaatat aactaaattc
     1261 tataacatta gaagaaattt taaatacctt tgataaaaaa tattgatgtt ttttctctca
     1321 ttgtttacaa actagatagc ttatgtattc ttattaaaaa taatagatat ttcgaacatc
     1381 atcactctca tctacttaat ttcgcatttt tactttataa aaaaactgat tctaagaaaa
     1441 ttaaataatt tttctcaaaa tcagtttttc tttgataaat aatattttca attatttaat
     1501 catttgaaaa ttgttagtca ttccaatacg ttgagaaatt cttccatctg tataaacacg
     1561 gaattgtttt ccaccagcca ttgttgtatt tccattatca tccgttacaa tattaattga
     1621 atagtagctt ccattttcat cttgcattag atctttcata tattcatttg tgctaattaa
     1681 attagtattt gatgcatttt tatcaggaga atcatacaac cataaagctt tattatttag
     1741 tacagtttct ttagcagctt cccaacttga aattgctgag ctacttgtag attgtgtcgt
     1801 tgtagttgtt gcttgttgag tagattcttc tactactgga gtttgaggag tttgtccatt
     1861 cacaatatca atgaaacctt gttttaactg ttggttctga gtttcattga agtacatgta
     1921 gtttttatca ttgccttgat tttgttgcgt taaataaact ttaggttgtc cattttgaaa
     1981 gccaaagaaa tatacatgct gccctaaata ttctccagtt tctacatcag aataaactgc
     2041 tactaaattg aaatctgcat gaccagttcc atcttctgac cattgtacgg taactggtgc
     2101 ttcattgatt gccattttcc aatttccatc aataactgca cttggtaaag gcgtaccata
     2161 cagacttaca ttgttctgag gattgtagct tttatattct tgacctaatg tctttcccca
     2221 ttgtgggaca aaaattgcta gttgagtaga tttctcacta tcccacaaag tatttttaac
     2281 ctcttcagaa gaagattcaa tgattgaact ttcacttgta cttgcttcca cagattgttt
     2341 catttctgtt gtagtttgtt cagttgattg cgttttactt tttttagcct caattgttgt
     2401 atcagtttct acagtctctt tagtcctcgt attctcagat gtacaaccag tgaataaaat
     2461 ccccattcca attagtaata acaaccattt tttcatttta tttccccctt tcaatttctc
     2521 cctcgcgtac ttattttatc agtcaattaa tctcaatggt gttcatttag ttaatccttt
     2581 cccaagaatg tcgatagcca gcatgacaac gatctcttcc ttgagggctt tcagatggat
     2641 taaaactagt agaaatttcc cttccacatc ctctacagcg ataagttctc attatttttg
     2701 ggctattagt attatttttc ttagttagag ctatgagtcc aactatgatt accccaactg
     2761 tcttaattac ttttccttta tctatattcg gatgcgcaag catatattta tataaccaat
     2821 atccaccgta aattaaacca cctaatacaa tcagcttagc catgtactcg tctcctttct
     2881 ttttgtttaa ttactttttg aatccatctt gcagagaaac cataaacttc tgtcaattga
     2941 cggatatcta cagttttttc ctgttctatc atgttttcga ttctttttct aactaactca
     3001 cgatcgtaca ttttcattgg aaaagaaatc tgttgccctc gaaaagcaag ataaaattga
     3061 tagagcaatt ctgaatccag ttccatttcc tcaaaaaagt cattcatact tttataagag
     3121 ttactcaaac aagtttcatc aatctccatc tacacacccc ctgttatttt ttacataatg
     3181 aaaatagcat attactgggt acattgataa ttcatataac atgtatcatc acatgtattt
     3241 ttaaagtcaa aaagcatgta aaactactta taagtaacat aagaaaataa aatgttattt
     3301 ttcttgaaat aataattgac tatctgccaa ttgccattca ttacgaacat gaatacttag
     3361 tgggtgggat aacttacctt ctgcatttat caatactttg gtcatctttt tttgtttcgc
     3421 actcaattga tcgtaaatgg tcttcgcttg ttcataagcc acttgactcg tcgctgtaat
     3481 cgaaccacaa gcaaaaataa tggtgttgat tttcttttct gtgaaaacag ttgttaatat
     3541 ttccaaagat tgttcatcat ttccacgagg aaatacccga ggacttttcg cttttgtata
     3601 cgaaaaaaga ttcacagcaa taaaaccagt aaatcctaat tgtcggatat tcttttcgat
     3661 taataacata ctcaaatcat tcgtaaatgg ttctgtatct gttggacgaa tagtaatgac
     3721 taccgctaat ttatcttccg cttcttttcc tttgatcgcc cattgtcgtt tgaaaacata
     3781 gcgatgggta tgatgaggat ctcgccaaac ttctgtttgt gtggtcacta attctttttc
     3841 aatcatctct ttttctccta tgctaatttt tgttcaagca attcaatcac cattgccgtt
     3901 ataagtgtca atactagttg aaaatatttc atattctttc tccttttttc ttcagaattt
     3961 gatgaatcgc tgtaaggata tcttctaaac atttcgcaat cattctcaaa taatcgatct
     4021 tattcattcg ttcctccaat ctgactccaa accaatcgtc cttctaccag taacccacga
     4081 acaaattctc gctggctggt aatatctgta atgaaataaa ctacatagtc ttttccatct
     4141 tctggtgcgc aataaatata aagatgtgtc atcactaaac gtggtagagt aacatcttca
     4201 ggaagcatac ccatttcaat ttcctctttt tccatcgtaa cggattcata gccccactct
     4261 tcaggagtag tgactagctg tgcacacttt tccagtactt cgatagccgt ttctaacaag
     4321 tcctttcgtt cctttctttt agatatcagt attcttctgg taataacatc gtttgatact
     4381 cacccgaatc aatgacccat attttttgag acactgattt caaacgaagc ttaggtgaaa
     4441 tcagatactc ttttcttgct tctggttctt cttgacgatg aaccaacttt tgatttcctt
     4501 tttccgtaga aaattcaaag atttgaagat agtctaaatg aaattgcttt tgaacgtttt
     4561 gatcgattag attccaacat aaagcttgta attccaccgg caaactgtcc attactccct
     4621 ttgtgacata acgttgtcct ttattttgaa acatttcttt tccctccttt gccataaaaa
     4681 tagcccaatc ttctattaaa gaagattggg cttcatggat ataatatata tttgatattc
     4741 aatttgattc attttatact atctgatttg tcaaattaag ctagacaata tgtctttatc
     4801 tatgcagctc aaaaatactt ccaatggtgt tcgataattt aatgattttc ttggaatatg
     4861 gtttcttttc gaggcaacgg attgaataaa ggtttcatcc gtgtccctaa agtccattgt
     4921 tttaggtaag ccatctcgtc taagcaagcc gtttgagtgc tcatttagcc ctctttgcga
     4981 aggtgttcct ggatctgcga aaaagatggc aatatcgttc gagttactta tagatcgcca
     5041 gttggaaaat tcttttccac aatcaaatgt catggattta aacaagtgtt taggcatgga
     5101 tttgaaccaa tcatttaacc tgttttcgat atcttcggct cgtctgcctg tcggtttcaa
     5161 agtaataata accttggata aacactcaac caatgtaata acagcgctct tgtgtttaac
     5221 tcccacaatg gtgtcgactt cgagatgacc aaattcttta gtcaggtgtg gatagagact
     5281 gttccgttca tgaatcgttc tgcgaaaggc ctgttttcca cgcttttctt gatgattatt
     5341 cggtttacgt ttccccttca taggtaacga ctgtgtgtca aaaaggcctt ttttaaacat
     5401 ccgatagagt gtacgaacag aacaatcaat tggaaactcg ccacggccga taatcacatc
     5461 gggtgaccat ccttgagcta ccttttcttg aacatatgtt gattgttcat cagataaaac
     5521 ggttgaacgt cgtccacaac gagatttatt ttgtttgtat tgacggtaat aatctagcac
     5581 tttttttcct tgtttgagaa acgaataaac attataaatg gtttgtctag agcgatttaa
     5641 actttgtgcg acaagagcga caggtttctc gatattgaaa taagactcta tgattactag
     5701 ttcgtctgtt gtaagatggg tgtaagtcat ttgtactcac tccttattaa ttctttggtc
     5761 ggaactattt tgagtgtagc acaaatgatc tttttagttg tctagcttaa ttttacaatc
     5821 gacgttttaa taaattgtga tataatgaag tataactttt tagggagtgt actatgaatt
     5881 ttcaagataa tagaccatta tatttacaag tagaagcttc tatacgtaac gatattcgtg
     5941 aaaaaaaata tcttccagga gagcaattac ctacagaaga agaattatgt aaaatttatg
     6001 gagtaagtaa gattaccatt cgaaaagctt ttaaactttt agcagaaagt ggaacagttg
     6061 aacgattaag ggggaaaggg acctttgtaa ctcaaaaaaa agaaagttta aaattagctg
     6121 gagttcatgg atttagcgat tctctttcta cccgaggaca taaaattaga tatactgttt
     6181 tacattctaa aattataaag gctgatgagt ttttagctaa taaattacaa atcaatgtaa
     6241 ctgatagagt ttacaatatt aaacgtttaa tgtgggaaga cggtgccccg atgggaattg
     6301 ataatttttt cgcatcagaa gaaaaatttc caactttatt tgaaaaaggt agccaatata
     6361 cttcattata taaacttcta gaagatgact atgatgtcaa agctacgagc tctattatgg
     6421 aaataaacgg tttatctgca acacctgagc tttccgactt attgcaatgt atagtaggag
     6481 atccgctatt tattttagaa aaggttggca aagataaaaa taatcaacca tttcattatt
     6541 ctcaatccac agttcgttgt gatagaatta gttatgttat tgaagtcaca gaaaatatca
     6601 ctttagatac ggtgcataaa aatgattcag atcctaaagt aatagattga tattaaaaga
     6661 aatcataccc aaagcaatca attttgatta gatttgggca tgatttttat ttttactaat
     6721 attctctaaa tacatcaaaa aacttattta tattccaaat ttcacctgta cttcttgtcg
     6781 cagatattta atatcgtaaa taacatcatc tgctagactt acatcttgag aaaaacgttt
     6841 atctttaatt gcaagaacct tcatatttgc tcttttacca gcttttatac cattataaga
     6901 atcttctatc accagacatt cttcaactgg aattctgagc tgttctgaag ctttaaggaa
     6961 tatagtagga ttaggtttac tttctggcaa atctcttccg ctaattaaac aatcaaaata
     7021 tgactctatt ttacaagtgg taacaacttc atttatagta tccattggac tggaagaagc
     7081 aattgccatt tttatttttt tcttttttaa ccattcaagc tgttcaatta gtatattagg
     7141 aaaaatatcc aagtaatcaa tcttttgcga tttccaatat ttagtaaatt ttcccttcaa
     7201 ttctaaactg ttacatccct cattgtactt ttctatatca tcaaaaatac ttgctgtagt
     7261 tccaatcttt ttttgataag aatacaaagg atagcttaaa ttttcttgtt ttaaaaaatt
     7321 aaatacccac ttttgataca taatctcgct atcaattaaa actccgtcca tatcaaaaat
     7381 tagagcctta atcattataa gatacctatt atacttaaaa caaaccctgc caaaatacaa
     7441 cctaataaga gcatatttgt actaacattt ttcttaatca atttatatat tcccaaagta
     7501 catacaagtg gtaataatcc aggaataatg gaatcaagta agtcttgtaa aacaagttct
     7561 gttccccctg tagtgaattt taaaggggtg acaattggca ccatgctagc caccattcca
     7621 cctactacag ccaaaccaat gatagaagca aattgcatta ctcgatccat aagaccttct
     7681 tgatacaatt tagtaaggta tttattgcca gactcatatc ctaatttacc gccgtaaaaa
     7741 acagctaaaa tagaaggtat agcaaaaagg ccaacagcta aaatcggtcc taaaatacta
     7801 ccttgcttag caaaagacaa tccaatccca aatgtaataa ttcttaaagt accttggaaa
     7861 aatgaatcac caattccagc aaaaggaccc attaaactgg ttttaatcat ggagatagat
     7921 tcagtattaa agtttccttc ttctgcttga gcattttgtt cttccataga agcacaaagt
     7981 cccataataa atgaagtcat ttgaggcgta cagttaaaaa atgccatatg acgagtataa
     8041 gcttctcttt ttttctcttc atcattttta taaactttat ttaaaattgg cgccatccca
     8101 tacataaatc ccatatgcat ttgtctttca taattccacg aacacatgat tgtccatgaa
     8161 cgaagacaca tcttattaag atcttttttt gtgattaaac gttttttttc tgtactattt
     8221 tccattaacc atgcgcctct tctctttttc catatttaac ttgatccatt acaaaggcaa
     8281 tcagagctgc aaaaattgtc acgccaatta gtgaaagacc tgaataggca acaataaaga
     8341 atccaaaaat aaagtaagga tataattttt tactcaacat tgtgcttaat agcattgcaa
     8401 accctaatgc ggaaagcata tttccagaga cagtcaaggc gtctgtaatc atttctggaa
     8461 ttttatctac taaatctgcc acaacgtcag caccaaaata tactgttaaa aagattggaa
     8521 taaagtaaac aaaagcatta aaaataaatg accaataaat atgaataatt tgagcttttc
     8581 taaatttatt attttcaatg gctttatctg cagcatgtcc aaattgagca attatcatgc
     8641 gcatgataat actcataaat tcaccagcta cagccactgg gatagctatt gttaaggctg
     8701 tttctgtact actattagtt aaaattgcaa aagcagtggc aataatacta ccaagttgca
     8761 tattaggagg tccagccgcc ccaattgaga cgaatcccat agaaattaat tctaaagatg
     8821 ccccaagaac aatccctgta tgcaagtcac ctagcacaag tccggcaagt gtactcaaaa
     8881 ttagtggtct accaatattt tgtcgaccaa ttaacctgga gtcaataaca gctaccacac
     8941 caactaaacc taataaaatg gcttttaata acatttattt tcccccctta cttactaaaa
     9001 tctacatttt caatacttga agatgggact tgttgcacaa atattttcac ttcttgatca
     9061 agaatttttt ttgtggcttc taattcttca ggagttaaaa agactgcctt tccatattgt
     9121 ttggactcgc ttttcttaga cacaccgcca aaattaattt ctttaatttc tggtaaattt
     9181 tgaactaatt caaaagcttc cttaggtcct ttaattaaaa tcatgtaatt atcatttggg
     9241 gatgtttcca taatcccttt aacatcacta attttaacaa tgtctaattt gcaatcagca
     9301 ggtttagcaa ttgtcaaagc catcacagac attggatctc ctggaacttt gttatcagcc
     9361 acaataatat ggttaatatc atactgttta gtccaagaaa atattacttg tccatgaagt
     9421 aatctgtggt ctacacgcac taatttaatc attctatttc ctccttcaat ttttaaaatt
     9481 tatagaatag aaattgccac cttaactgac atcttttaaa agcgaattaa tatgaaccat
     9541 acttttttga gagttattca gtaattcctt aaaatctatt tcttctatag atttaccagc
     9601 tgactgggcc attaataact ccagtaaaag aggtaaatta atcccagcaa taatttgaat
     9661 tctattgttg ttcaactgtt taatagaaac attattaggt gttcctccaa ttaaatccgt
     9721 taaaataatt agaggttgca aatgatgctg actaattatt tctttcatac tctcctcaaa
     9781 atcatctgga ctagtatttt ctttaaatgg aacagcacta atacctgtgg cgtcattcat
     9841 taaaattaat tttgttgcct ttaaataacc ctcacaaagg gtagaatgtg ttgtaatcac
     9901 aatgtgaaat aattgatttg ccatcttttg caacctccta ttaattaata tttcatttgc
     9961 cacatatagc gtctaacatc taaggaatgg cctctttcaa aagcaatcgc ttctaccatt
    10021 tggcgaataa caacgccact aagtacagct gcaatatatt ctttcgctac actatctata
    10081 gattctaagt caaaatcagc ttggtctaaa accattaatt tattactgaa ttttttagtg
    10141 aagtcttcta cccgttgatc taaaaatctt gtatctccaa tacttttcac taagatgaat
    10201 ggtacatcat aatcagtgat ttcaaaagga ccatggaaaa attccccaga atgaatagca
    10261 ttagaattaa tccattgcat ttccatgaac cagcaagctg ctgtggagta aatttctccg
    10321 taattgatac cgctaccaat tgtgtaaata attttatctc gtttttggtt tttaccccat
    10381 tctttagcag ttatttcaaa tttgctcttt tcagcaataa caagatcatt taacttatca
    10441 aattcattca agcaagtttc ccatttttta gagccatcta aaacaagtaa aatattaaat
    10501 aataaaccat aaagaattcc tttatttaaa tcactagcat caacatcttt accccaatca
    10561 tagtgaacag gatattcagc tgcttcccat agaggagatc cttcttcatt agataaacaa
    10621 atagtcattg ctccaaattc tcttgctttt tttgttgctt ctacagtttc tggtgttgtg
    10681 ccagaatggg aacaagtgat aactaaagaa ttttcagata atccttttgg tatagcatag
    10741 ttaaattcgt tagcagtata aacatgacaa ggtacagtta attctttatc aaatagatat
    10801 tgaccagcca ttaaaacggc ttgtgaaccc ccacatgctg taaaatatac atgatcaatt
    10861 gtcttttcct ttaacgctgc cattgctgtt tcaatctgtt ctttgtgtag ttgtctcatt
    10921 tctttatccc tcactctaat aagtattata aagtcatata actttacaat acctattatc
    10981 tatgaaattg ctttattttg caatactatt tttcaaaaaa atttttttat aacaatttta
    11041 tataataaaa tg
//
LOCUS       DAZBTF010000086        10746 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000086 DAZBTF010000000
VERSION     DAZBTF010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.087"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            157..972
                     /gene="thiD"
                     /locus_tag="ACHGNS_002460"
     CDS             157..972
                     /gene="thiD"
                     /locus_tag="ACHGNS_002460"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFN6938356.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(1301..2752)
                     /gene="cls"
                     /locus_tag="ACHGNS_002461"
     CDS             complement(1301..2752)
                     /gene="cls"
                     /locus_tag="ACHGNS_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFN6938357.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            3093..5759
                     /locus_tag="ACHGNS_002462"
     CDS             3093..5759
                     /locus_tag="ACHGNS_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFN6938358.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            6123..6875
                     /locus_tag="ACHGNS_002463"
     CDS             6123..6875
                     /locus_tag="ACHGNS_002463"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN6938359.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(6897..8051)
                     /locus_tag="ACHGNS_002464"
     CDS             complement(6897..8051)
                     /locus_tag="ACHGNS_002464"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFN6938360.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            8236..10677
                     /locus_tag="ACHGNS_002465"
     CDS             8236..10677
                     /locus_tag="ACHGNS_002465"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFN6938361.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         3501 a         1912 c         2305 g         3028 t
ORIGIN      
        1 actattttct gatttattgc aaatgttttt tcaaataaat ttgattacca agaaaaggta
       61 gtggtatatt tactgaggga tactttgaaa aacatatgat agaaacagta acagtattat
      121 gaaaaatctt tttataaaaa tggaggaatc gatttgatgg aagatcagta tgtacaagca
      181 atgacgatcg caggatcaga tagcgatgga agcgcaggga tgcaagcaga tatgcataca
      241 ttctttacaa gaaatgttta cggtgtttct gtcatgaccg catgtgttgc aggtaattct
      301 tacggaatcg gagcaagtgt gacattgcca acagatttta tcgacaaaga atttgaactg
      361 attgcgaagg actttcaaat acgtgcagct aaaacaggta tgctagcaga ttcaaagttg
      421 attgagacag ttgtaaaaaa ttaccaaaaa tatgattttg gaccattagt cgtggatccc
      481 gtaatcgtaa cgaaacatgg aaatctttta ctagaagaat ctgctttgca aagcttgaaa
      541 gaaaagcttg tccctttggc cgaagtactg acacctaatt tttatgaagc agaaaagctg
      601 actggacgga cattagcagc tgaagaagat tttgtgcaag cagcaaaaga actgcaagat
      661 atgggtgcaa aaaacgttat gatcaaagga catcatgcta aaaatgcgca gcaagaaaca
      721 gtcaatgact acgtattgtt cgaatcagga aaatcatttt ggttaaatgc accattttat
      781 cctaccgacc ggatcaacgg aactggcgat tcgctatctg catgtattgc agctgagtta
      841 gcgaaaggaa aaacagttga acaagcgatc cgattggcaa aagactatgt aaaccgtgca
      901 attcgtgaag aaataaatgt agggcacaaa tatggcccca tcaatcattg ggcagctggt
      961 gaattagagt aaaagaaaga agttgtgaaa aagttgccct gcatcaagta ataagaacag
     1021 ccaagtaaaa acagcttctt atgtttttaa cttggttgga cttattaccg cagatgcaag
     1081 cttttgaaca ccgtttaaga aagaggtcgt gagcctgccg tccagctccg agtcacaagg
     1141 aacaagttca aaaaacagct tctcatgttt gttgaatatt gtgacttgtg accgaggagt
     1201 tggcaggaga acaccgtttg ttcggaagaa gggactgtga caagattttt gtcacagtcc
     1261 ctttaatctt agtatatttt tctctacttg aataaaaaaa ttataggatt ggagaaaata
     1321 atcgagaaat ttgttgcttg aataatagcc agttagacat ttccttgact gtttcagttg
     1381 tcatggatga acaatgtgtc tcatcttctt tgaactgagt agcaagttct tctaaaaact
     1441 caggatcgta aatgacagca cttgtttcaa aattcaatcg gtagctacga atatcttgat
     1501 ttgctgaacc taccatgcag atttcatcat ccattatcaa tgtttttgca tggaggaagc
     1561 cgttctcata gacaaggatt tgtacatttt ccttgatcag ctgccgagca taatattgtg
     1621 ttgctcgata aataaaagga tgatccggtt tgttgggaat catgattttc acatctacac
     1681 cagaggcagt tgcgattttt aaagcagcaa tgacactttc atcaggaaca aggtaaggtg
     1741 tctgtatcca aacacgtttt ttagcagaag taatcaattt gataaatgaa agcttgattt
     1801 gttccctttc gttattcggt ccgcttgaaa caagctggat ggatgtatca gcaagcttag
     1861 attcatctcg atctgctttt ttaaagaaat aattcaaatg atagccgacc tttttttctt
     1921 caggtaccga gacgttccag tctgttaaga agcgaagctg gagcaaagaa gaagcggcac
     1981 cgaaaatccg tatatgcgta tcgcgccaat agccgaattt ttttgttgta tttacatatt
     2041 gattggcaat attgaaacca ccggtataac taatctttcc atcgatcaca acgatttttc
     2101 ggtgatcatg gtaattcaat cggaaacgaa gtaatgccct ttgtgaggta acaaatgtat
     2161 ggacatgtcc accatttttg attagacgat tgaaatcttt tgcttttgtg ccatgagagc
     2221 caaatgcatc atataatatt cgaacttcca cgccttctgc ggctttttct tctaaaacat
     2281 gtaagacttg ctggccgata ttatctgtca caaatgcgta atattcgata tggatcgaat
     2341 gtttggcttt ttttagatct tgaagcagtg aatccaattt ctcttgtccg tctgtgagaa
     2401 gagtgacaga attcattctt gtcagcggca tacgatttaa tgaagagaag aagtcaacaa
     2461 attgctgttt gtctttatcg cccatgtcag gatcgtaatg ttcaatggta tctcctctaa
     2521 aagactgaaa gttttctaat tctttcaagt cactttgtcg gagatagaat tttttcttat
     2581 ccgttaatcc acgtccgaaa aataaatata atacaaagcc caccccagga agggcaaaca
     2641 atactaagag ccatgcccaa atggctgcta catctcgggg tttgatcaaa atagccacca
     2701 aagcaataag tgcatttaat agataaaggg cggttataat actagatacc acaaaaatgt
     2761 tcctccttac atacaataac attagtatag catgatagaa gaaagaagcc taccaaggtg
     2821 aatatacttt tcactccttt catgaaaatg taggtaaatc gggctaaagt agttgctttt
     2881 tgtaggttaa taaggtagga tattagtaca gttgaatatc tccttagggg agtaaccggc
     2941 aacgtttgtt gcgataatat caacagtaag ttaaccaaat ggttttctgg tattgtatga
     3001 aatggtgaga cctaagacaa atgcgtattt gtcttaggtc tctttttttt cagacaggac
     3061 tagggccaag atcaaaaaat gggggatgaa atttggaaaa caaggaacaa gcacaacaag
     3121 cggtctataa agaaacgatc gactcgttga tgcagcaaac gcagagctca accgatggat
     3181 tgagcaccac gcaagcgaag gaaaaattag aaagagatgg cttgaaccag ctaaaagaaa
     3241 agccgaagaa gccaacttgg aaattgttct tagaaacttt caaagatgca atggttatcg
     3301 tattgctgat tgtcgcactg attcagatgg tcatgggtga ctacatcgaa ttcttagtta
     3361 tttttgcagt attgatgttg aattctataa tcagtgttgt ccaaacaaaa aaagcagaag
     3421 gttctttaga agcgttgaaa aacctttcag cgcccgatgc tcgagtgatt cgtgatggta
     3481 aggaaaaaac gattccggca aacgaacttg tggtaggaga tatcgtgttg ttagacgcag
     3541 gcgattatgt acctgctgat ggacgtttac tagatgcggg atcattaaaa gtagatgaag
     3601 gtatgctgac aggagagtcg acttctgcag aaaaagaagt aacggatatc acaagtacgg
     3661 taccaatcgg agatcgtaca aatatggtat tcagtggaac gatcgtaact tacggacgcg
     3721 gtaaattctt agtgacttca actgggaata atacggagat cggacaagtt gctggccttt
     3781 tggaatcaac aactgagcaa gtcactccgt tacaacgagg tctggataaa ttcagtaaaa
     3841 aactaagttt ggctattttg gcattgtctt tattgatttt aggtatccaa ctgtttcgta
     3901 tttatcttgg agaaggtaca ggagatatga ctgcagatat cgtcagcgca gtgatgtttg
     3961 ccgtagccgt agccgtagct gctattccag aagcattaca gtctatcgta acaatcgtgt
     4021 tatctttggg aacgaataaa atggcaaaac gtcatgccat tattcggaag cttccagcag
     4081 ttgaaacact tggttcgaca agtatcatct gtacagataa gactggtaca ttgacacaaa
     4141 acaaaatgac agttgttgac tactttttag gtaatggcga tactggggat ttcacaaatg
     4201 acccttcaaa atggacagct gacgaacgtc gcttgatcga aatcagtgtt ttagcgaatg
     4261 atgcatcgat cagtgaagac ggtactaaat tgggcgaccc tacggaagta gcgtttatcg
     4321 actttagtga aaaattaaac cagccttatc aggaaatccg aaacaactac ccaagacaag
     4381 cagaacttcc ttttgactca gatcgaaaat taatgtctac tggacacaca attgataaca
     4441 aaaattactt gtttgttaaa ggcggaccag atatcgtctt ccaacgcagt aaaaaagtat
     4501 tgctaaatgg tgaagtcgtt ccgatgacgg atgaattgat gaaaaaattc caagagcaaa
     4561 atgaagcttt ttcaacacgc gcattacgtg tactagcttt tgcttaccgt ccacttgata
     4621 attttgattt atcatttgaa gatgaagacg atttgatcct tgttggtttg cttgctatga
     4681 tcgatcctcc acgtgctgaa gtgactcagg cggtaacaga agcaagaagt gcaggaatca
     4741 aaacaatcat gattactggt gaccacaaaa caacagctgt tgctatcgca aaacaaatcg
     4801 ggatttttga agaaggaaac ctggctttga ccgggacgga actagatgaa ttgacagaag
     4861 atcaactaaa cgaagtttta gaaaaagtca ctgtttatgc acgtgtttca cctgagaata
     4921 aaattcgtat cgttcgtgct tggcaaaata aaggtcatat ctcagctatg acaggtgatg
     4981 gtgtaaatga cgcaccagca ttgaaacaag cagacatcgg aattgctatg ggaacgggta
     5041 cagatgtggc aaaagatgca gcagcgatgg tattgacgga tgataacttt gcttctattg
     5101 tcagcgcagt agaagtagga cgtaatgtgt ttgataatat caagaaagct atttcttatc
     5161 tatttgcagg aaatttaggt gcgatcattg cgatcatctt tgccttagtt gctgactggt
     5221 ccaatccatt tacagcatta caactgttgt tcatcaattt agtcaatgat tcactgccag
     5281 caattgcatt aggtacagaa aaagctgaac ctgctacgat gaaccgcccg ccaagagatc
     5341 caaattcagg aatctttacg gacaaaacat tgatttctgt aacgtatcgt ggtgtgctga
     5401 ttgggattgc tgttatcttt gctcaatggc ttggcatgca gcattcacct gaattcggtg
     5461 tagccatgtc attttcaaca ttgatttggt gtcggacatt gcaaacattc ccagcacgtt
     5521 caaacacaca aacagctatc caagcagggt tcttctctaa cagaagtgtc ttactagctg
     5581 tagttgtatg tagcgcgctt tactgcttaa caatgatccc aggactgaaa gaagtcttct
     5641 ctatcccatc agcatttgga ttgacagaag tctttacatg tatgggaatc gcacttgtga
     5701 caattgtttt gatggaaatc actaaattga tcattgtacg agtacaagga aataaataaa
     5761 acgaatcaac gaggttgtga gcctgccgtt taattctgag tcacaaggtg caatttcaac
     5821 aaacagcttc tcatgtttgt tgaatattgt gacttgtgac cgaagagttg gcaggagaac
     5881 accgtttaag gaagaagttg taaaatcagc attttgagct aatactgagg gtcatctgat
     5941 tggacactgt ttattcggaa agagggactt tgacacgact ttggtcacag tccctttttt
     6001 tatttatgat agacggaaag ccaatcattt tattattcat caaaagagat agaatagata
     6061 aaagaggaga atgatttatt ccgaaaagtg aaaacatata acaaaaaatt agaggaaatc
     6121 aaatgaatga aaaagaatgg gatgaatttg ctgaagaata ttatgaaatc cagcaagaat
     6181 cgcaagtaac tattgctgaa gatgtaaaaa attatctgaa aacggagaaa gtcctgcctg
     6241 ctgaaacagc agcagacgtt gctggaggat ctggcagatt tctttcctta gcaaaagaaa
     6301 ttaatcgcta tgatttgatc gatttttctt caggaatgct agaaaatgca aaaaaagaag
     6361 cagaaaaaaa tgagttgcca aatgtttcct ttatcaaaca gtcgttagaa gaatttatcc
     6421 aatcaaatga gacatatgaa atggttttta cagcagcaaa tccagcattg gtacatctta
     6481 ttcaattaga aaaattgatg aaaaagtcaa gcaaatggtg cgtgataatc agagtagtgg
     6541 aatcaatcga tgatgttttt tatcctatcg aaaagcaact aggaatgata gaagaagatc
     6601 ctaatacatc gccagttgta atggatcaat ttgaaacata cttaattgat gaaggattca
     6661 aagtgaagaa aaaagatttt acttatttta cacaggaatc aattactgaa gaattgatga
     6721 agctatacta tgacgcgtac gcaaaagatc ctgttgtgac caggtacatt cgcaaactat
     6781 tcagtgaaaa acgagaaatc cttagtacga ctaagctgac ctatagactg ctcctttggt
     6841 ccgcagataa aaaaagaacc tttcacgact cgtgaagggt tctttttatg attttattat
     6901 attttgtagt aacgaatatc attttcgatc tttgtaatcg aaaaaggtac atctgaagta
     6961 tattctgcac attctccgtg ttcaggaagc gtctcactga aaatcgtttc atattcgtca
     7021 acagtgatcc gtgttcgaga atctagcatc tcttgatgag attgtgcgaa gagatattct
     7081 tgatagtttt cttccagata acctgtaaaa aattcactta ctgcaccaga accgtagctg
     7141 aatagaccga tgcgatctcc tggctgtagt gatttcgaat tttccagtag agaagttagc
     7201 cccaggtata atgaaccagt gtaaagatta ccgattcgtc ggctgtaacg gatgctttct
     7261 tcatagcgag ccataagacg ttcctgattt tcttcgtctg tttggtctaa tacgctttgc
     7321 aatgcctttt ttcccatctt agtatacgga atatggaaag caatcgcctg ataatcttcg
     7381 agtcctcgac ccgacaattc tttatgtcga ttccaaactt tttggaatga ttcgatatac
     7441 gtagaattag ataaaggacc atcaacaaca ggaaattcgc tataatctgg acgccagaaa
     7501 tcatagatat cttctgtcag aaatacgctg tcgtcttcaa tcgataaaat acgcgggttt
     7561 tgagtgatca tcatcgcaac agcaccgaca ccttgcgtca cttcaccacc gcttgccaag
     7621 ctgtaacgag caatatcact tgctatgact agtacttttc gttctggatg atttttgaca
     7681 tattctttcg ccatgtgcaa agctgcggtg ccaccgtagc aagcctcttt tacttcaaaa
     7741 gaacgagcaa aagattggat tttcagtaaa tggtggataa ttaccgcact cgcttttgaa
     7801 tgatcaatcc cggattctgt tccgacgatg accatgtcga ttagctcacg gtctttttct
     7861 gttacgattt tgctggcagc atttgctcct agtgtaatga tgtcttcatt tgcacgattg
     7921 actgccattt ggtcttgacc aatccctata tggtattttg caggatcatc cccacggctt
     7981 tctgccaact ctgtcatatc taaatataaa ttaggaataa aaaaggaaag acgatcaatc
     8041 cctattttca tttcaacacc tctttactta tgataaaaaa cttactttac taaggtatca
     8101 taaaaagatt aaaagaaata taaaaaagag aatatttaag gattgttaaa atttaatctt
     8161 agaaaaaaat aactggaaca agagctattt gtttttacag caaagaatgg tatatttttg
     8221 taggaggtgg cttacttgaa agaagtcgtt atgatagatg ctgcaagaac gccgatagga
     8281 aagtatcgcg gaagtttaag tccatttact gcagttgaat taggcacact tgtgactaaa
     8341 ggattattag ataaaacaaa actgaaaaaa gataaaatcg atcaagtaat atttggaaat
     8401 gtcctgcaag ctggtaatgg tcaaaatgtt gccagacaga ttgctttgaa cagcggtctc
     8461 cctgtagatg tcccagcaat gacgatcaat gaagtttgcg gatctggtat gaaagcagtg
     8521 atcttagcac gccagctgat ccaattaggt gaagcagaac ttgtgatagc cggcggaaca
     8581 gagagtatgt ctcaagcacc tatgctgaaa cagtatcagt cagaaacaaa tgaatatggt
     8641 gaaccaattt ccagtatggt caacgacgga ttgactgacg cattttcaaa tgcacatatg
     8701 ggattaaccg cagagaatgt tgcaacacaa ttttctgtga gcagagaaga gcaggatcgc
     8761 tatgccttgt cgtcccagtt gaaagcagca catgctgtcg aagccggtgt attttctgag
     8821 gagatcatcc cagtcaagat ttctgatgaa gacgtgttat ctgaggatga agcagttcgt
     8881 ggaaatagta cattggaaaa actgggcacg ttacgtacag tattctcaga agaaggaact
     8941 gtaacagcag gaaatgcttc cccgttgaat gacggtgcct ctgtggtgat ccttgcatcc
     9001 aaagaatacg cagaaaataa taatctgcct tatttagcaa ccatcaaaga agtagcggag
     9061 gtcggtattg acccaagtat catgggaatc gctccaatca aagccattca aaaattgaca
     9121 gatagatcag gaatgaattt atcaacaatc gatctttttg agatcaatga agcatttgca
     9181 gcttcttcta tcgtagttag ccaagaactg cagttagatg aagaaaaggt caatatatat
     9241 ggaggagcaa tcgcattagg ccatccaatt ggtgcaagcg gtgcacgtat cttgactaca
     9301 ttagcttacg gattgcttcg tgaacagaag agatacggta ttgcttcatt atgtattggt
     9361 ggaggtttag gcttggcagt cttgttagaa gcgaatatgg agcaaacaca taaggatgtg
     9421 caaaaaaaaa agttttatca gcttacgcca tctgaacggc gcagtcaatt aatagaaaaa
     9481 aatgtattga cacaagaaac tgcgttgatt tttcaggaac aaacactttc tgaagagctc
     9541 tccgatcaca tgatcgaaaa ccaagtgagt gaagtggaaa tcccaatggg gatcgcgcag
     9601 aattttcaaa tcaatggcaa gaaaaaatgg attccgatgg caacagagga accatctgtc
     9661 attgcagctg caagtaatgg tgcaaaaatc tgtggaaata tctgcgcaga aacacctcag
     9721 cggctgatgc gagggcaaat cgttctatca ggaaaatcgg agtatcaagc agtgatcaat
     9781 gcagttaatc atcgaaaaga agagctgatt ctttgtgcaa atgaaagtta tccttcaatc
     9841 gtgaaacgcg ggggcggtgt tcaagatatc tctactcggg aattcatggg gagtttccat
     9901 gcatacttat ctattgattt tttagtggat gtaaaagatg caatgggcgc taacatgatc
     9961 aactcgattc ttgagagtgt ggcaaacaag ttacgggagt ggttccctga agaagagatt
    10021 ctattcagta ttttaagtaa ttttgctaca gaatcattgg cttctgcctg ttgtgaaatc
    10081 ccattcgaac gcttagggag aaacaaagaa atcggtgaac aaattgccaa aaaaatccaa
    10141 caggcagggg aatatgcgaa gcttgaccct taccgtgcag caacgcacaa caaagggatc
    10201 atgaatggta tagaagctgt agtggcagct acaggaaatg atacacgtgc ggttagcgct
    10261 tcgatccacg cctacgcggc aagaaatgga ttataccaag gattaacaga ctggcagatc
    10321 aaaggggata aactagttgg aaaactaacg gtgcctttgg ctgttgccac agtaggcgga
    10381 gcttccaata tcctgccaaa agcaaaagca tctttagcta tgttagatat tgattcagcc
    10441 aaggaactag cacaagtaat tgctgcagta gggttagctc aaaacctggc tgctttgcgt
    10501 gcattagtta cagaaggaat ccaaaaaggc cacatgggac ttcaagctcg ttcattagcg
    10561 atctcaattg gggctattgg tgaggaaatc gagcaagttg cgaaaaaatt acgtgaagca
    10621 gaaaagatga accagcagac agctatacaa atattggaaa aaatccggga aaaataaaaa
    10681 agcaatatct ttagaagaat aaagcaagat ttacagttat tttatttact gcagatcttg
    10741 cttttt
//
LOCUS       DAZBTF010000087        10656 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000087 DAZBTF010000000
VERSION     DAZBTF010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.088"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..656
                     /locus_tag="ACHGNS_002466"
     CDS             33..656
                     /locus_tag="ACHGNS_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938362.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /gene="trpX"
                     /locus_tag="ACHGNS_002467"
     CDS             1106..2155
                     /gene="trpX"
                     /locus_tag="ACHGNS_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN6938363.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="ACHGNS_002468"
     CDS             2152..3033
                     /locus_tag="ACHGNS_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6938364.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="ACHGNS_002469"
     CDS             3036..3806
                     /locus_tag="ACHGNS_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6938365.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="ACHGNS_002470"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="ACHGNS_002470"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFN6938366.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="ACHGNS_002471"
     CDS             5117..6280
                     /locus_tag="ACHGNS_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFN6938367.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="ACHGNS_002472"
     CDS             6397..6807
                     /locus_tag="ACHGNS_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN6938368.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="ACHGNS_002473"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="ACHGNS_002473"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFN6938369.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9380..9664
                     /locus_tag="ACHGNS_002474"
     CDS             9380..9664
                     /locus_tag="ACHGNS_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938370.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9824..10060
                     /locus_tag="ACHGNS_002475"
     CDS             9824..10060
                     /locus_tag="ACHGNS_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFN6938371.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10057..10455
                     /locus_tag="ACHGNS_002476"
     CDS             10057..10455
                     /locus_tag="ACHGNS_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFN6938372.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3723 a         1773 c         2253 g         2907 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     9541 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     9601 ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg
     9661 ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt
     9721 tcgtaagaaa aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa
     9781 tgtacttact atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa
     9841 gtataaaatt agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga
     9901 attcatgggt gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt
     9961 acgaatgatc aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa
    10021 tcaatatgaa gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa
    10081 attatcaaaa tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta
    10141 aaagatgcaa atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa
    10201 gaactctatc ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa
    10261 catccttttc ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa
    10321 ttgaataatt gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt
    10381 accactagtt cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa
    10441 accgcaataa aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa
    10501 ggaatatgct gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga
    10561 aaatatggat taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg
    10621 aaatagtggt ttagaactag tgggctgatt ttttat
//
LOCUS       DAZBTF010000088        10623 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000088 DAZBTF010000000
VERSION     DAZBTF010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.089"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            112..279
                     /locus_tag="ACHGNS_002477"
     CDS             112..279
                     /locus_tag="ACHGNS_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFN6938373.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(341..2152)
                     /locus_tag="ACHGNS_002478"
     CDS             complement(341..2152)
                     /locus_tag="ACHGNS_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFN6938374.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2149..2514)
                     /locus_tag="ACHGNS_002479"
     CDS             complement(2149..2514)
                     /locus_tag="ACHGNS_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFN6938375.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2677..3072)
                     /locus_tag="ACHGNS_002480"
     CDS             complement(2677..3072)
                     /locus_tag="ACHGNS_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFN6938376.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(3090..4052)
                     /locus_tag="ACHGNS_002481"
     CDS             complement(3090..4052)
                     /locus_tag="ACHGNS_002481"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFN6938377.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(4052..4264)
                     /locus_tag="ACHGNS_002482"
     CDS             complement(4052..4264)
                     /locus_tag="ACHGNS_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFN6938378.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4285..4983)
                     /locus_tag="ACHGNS_002483"
     CDS             complement(4285..4983)
                     /locus_tag="ACHGNS_002483"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFN6938379.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(5003..5545)
                     /locus_tag="ACHGNS_002484"
     CDS             complement(5003..5545)
                     /locus_tag="ACHGNS_002484"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFN6938380.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5677..6681)
                     /locus_tag="ACHGNS_002485"
     CDS             complement(5677..6681)
                     /locus_tag="ACHGNS_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFN6938381.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6678..7667)
                     /locus_tag="ACHGNS_002486"
     CDS             complement(6678..7667)
                     /locus_tag="ACHGNS_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFN6938382.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7664..8470)
                     /locus_tag="ACHGNS_002487"
     CDS             complement(7664..8470)
                     /locus_tag="ACHGNS_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6938383.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8636..9592
                     /locus_tag="ACHGNS_002488"
     CDS             8636..9592
                     /locus_tag="ACHGNS_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN6938384.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9669..10187)
                     /locus_tag="ACHGNS_002489"
     CDS             complement(9669..10187)
                     /locus_tag="ACHGNS_002489"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFN6938385.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10275..10424)
                     /gene="rpmG"
                     /locus_tag="ACHGNS_002490"
     CDS             complement(10275..10424)
                     /gene="rpmG"
                     /locus_tag="ACHGNS_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFN6938386.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
BASE COUNT         3308 a         2314 c         1812 g         3189 t
ORIGIN      
        1 gtaaacattt tcaaggattt tttgcaaagg tttacaaaca aacgtaaaac tagccttttt
       61 ttctcttttg ttccataata gatataacaa ataataaatg aggtggattc aatgaaaaaa
      121 tttgtatctg gaatattagt aggaagtctg gcaacagccg ctgccgttgc tggtttggtc
      181 gcttctgtga aaaaaacagt gatcgatcca attgacgaaa aagaagcaat gatcgaagaa
      241 aaccgtaaaa aagcaatgcg caaacgcgtt tctcgttaat aattatttaa aaacacgcat
      301 gaagacttgt gaaagaacag ataagtcatc atgcgtattt ttatttgtca gtcaatataa
      361 gttgtgctat tttatctgcc tgtctcagat tccatggatc tgtataaggt ctagctactg
      421 ctgcagttag ccaatccaca ccttcaacag gtattccata gcaataaaag tcagactgga
      481 tttcccctga agcatcaatc acgcgattcg tacctcgctc aacatctata gcttctgaat
      541 gataacttcc tgtattatct gaaaatgaaa aagggcgaac aagtttgttt tccttcaaat
      601 tttttatgac aggattactt gaacgagaaa agcttgtttg tggcaatcga gcttcaatca
      661 aatagcgact gctgatttct ttatcttgta gagaaataac atagcggttg tgcttttgct
      721 gaatatcaaa tggcggttcg aacaaatgaa aaatacccgc atctatcagt gctgctaatt
      781 ctttcgtccg aatgactggc ggaccgatcg ttaaaaagct atttagatga gtaaaccatc
      841 cccatagtat ttctttgtat tcctttgcag aaaaaacgtg ccattcaata gcctgccgga
      901 ttggatcacg ccagtctttt aatgcatcaa atgttgaagc aaatgctcct gtgcaattgc
      961 ctttttccgc ttcttttatc tgcccttcaa taaatgctcg gctagcttct gcaaaggact
     1021 catcctgata ggtaagagga atttctaaat acgaccaatc ccatctataa gggattagtg
     1081 ctggatgctt tccgagacac tcctctgaag agagggagag aaaatcatgc tcaaaatttt
     1141 tctttggaat cggcaaatga tattcttcga tgattttctt gtaataaaac aactcaacgt
     1201 cttttttcag atagtcaaaa aatgtttcgc cagtcaaaaa tcccgactga tgccattgct
     1261 ccaggttttc ttttgttagt aaccgtggcc atgccattga tccgccttgc ttttggtttc
     1321 ttcctcgagg gtaataaggg agcccccttc ttgatccact gtatacaatc ggttccttgc
     1381 ctgatggatg atagacaagt tttccttttt cgttctcgaa gcgcccttct cttgatactg
     1441 taaacaatcc gacataatca aaaaaactta agcctagacc tcttaaaaag acattttctt
     1501 taggcggtat cttcgaaaga tcgacatctg caggattaga aggaagctga tacagtaaat
     1561 catttttctt tgcgtagtct acccacactc tttcttcttc tgtctcatca ttttcccagt
     1621 gaccgcttgc taaaacaagc tgatcgatag aatagctttg ctgatctgtc tgtaaaagat
     1681 aaccatcttt tcttttgctg acttttttta ctaaagaata aacaaactga atagaagctg
     1741 gaaattcttc ttgcaacttt gagaaaaacc agcgttgata gagtccgtat aaacatcgag
     1801 tagattgctg atctttttcc agacgattga tttcttctat aattagttgc ttatttttgg
     1861 ttaccgtctg ttcgatataa tctgtagcag gtcctttact ccattgaaac aagttgggtc
     1921 cctgtgttac caatcctcca ctggacaatg tttcatctgt aaaaagcgtc acttgttgag
     1981 aaacagaatt cattaataac tccggtggct gattcaaccg ccaaactcga cctccaggac
     2041 cattgggatc gaaaagaaaa agttcaagag aatgtctgtc ctgattatta tggagaatcc
     2101 tttctgctat acttaatcct ctaggtccgg caccaatgat ccctattttc atttgcatgc
     2161 ctccatttcg ataaattttt tcaataaaac agcatggttc tgatgttcat tttttgcacc
     2221 ataaacaaaa gtaatgactg gatgttcttt cataagttga ctgagtttaa aaatagcttg
     2281 cttcttttct tcttcgaaaa tcaattcttg ctggtatttc tttgcaaatg aagcaaatcg
     2341 ttctggtaca tggtcaaaag ttttgcgtgc ttcattacta ggtgcaattt ctttcaacca
     2401 cagatctaac tgcgcttttt cttttgaaat tcctcgtggc cagaacctat ccacgagtat
     2461 tctatagcca tcctcttttt cagcatcaag gtaaacacgt tttaatttga tcatagttgt
     2521 ttccttcttt ctgccttttc gcttagctta tatatgtaga taatagcaca aaataaaaaa
     2581 aatgaacggc acaactatcc attgcgccgt tcatgtgatc ttggtcaaaa tctaatgatt
     2641 gattcggttt ttaaagctgt catacaatca tctgttttat acttttttac ttttgatttt
     2701 tccagaccat ccgtcatatc cacccttaag taaataaatc tcttgataac ctgctttacg
     2761 taatttattt gctgtacgga tgctcaagct tttcttttga tcgtaaatat agacaggctg
     2821 gtcttttcga atagatccta atgaatcttt caaaacagtg taagggatgt ttcttgcacc
     2881 taaaatatgc ccagcatcaa acacatcttt ttcgcgaaca tcgattactt gtgcttttct
     2941 cattccttct cgaaattctt cttctgtcaa agtcgttgct gaacgcttgg ccattactcg
     3001 tagatagact tcccaaaaga tgattgcaag cacaatcaat agcaaaatga tattaattac
     3061 ccataaaacc atgtaatgcc tccgtttttt taagcaaatt tcaaggctaa tgaggcagca
     3121 ccaatgacac cagcttcatt accaagctct gctaatttga tttttgttga ttctcttact
     3181 tgcgggaaag taaattcttt aaaatatttc tctacacggc tacgtaaaaa ttctcctgct
     3241 gccgatactc ctccacctaa cacaatactt gacggattca atgtattccc caagttggcg
     3301 caagctaaac ctaagtagaa gcatacgcga tcaacaacca tcaatgcaaa cggatcgtct
     3361 tcttgagcca tttcaaagat atctttactt gttacttctt caccgttgtc taacattgct
     3421 ttcagttttg aatctcctgc gtattcttct gccaagtaac gacctagacg taccacacca
     3481 gtagcgctag acacagtttc taaacatccg cgtttaccgc aagtacattc gaacccttca
     3541 ggatccacag tgatatggcc gatctctccg cctgctccgc tcacgccgtg aatcaaattt
     3601 ccgccagcaa cgataccacc gccaacacct gttcctagag taacgaatac aacgtctgga
     3661 tcgttttctc cagcaccttt ccatcgttca cctaaagcag caacattcgc atcattatcg
     3721 atagcgaatt tgattcctgt tcctgcctcg atcagttttt tcacaaattg aacttctgtc
     3781 cagtttaagt tatacgcacc aataaccgtt ccagcttcgc tgtccacgct tcctggggta
     3841 cccatgccga ttccgataaa gtcttcggct ttcatattat ataaattcaa acgatggttg
     3901 attgattcaa tgatttcagg aacgatatgc ttaccatcat ccaaaatatt ggtatcgatg
     3961 ctccattttt gttgaatctc tccttctggt gttaaaattg caaattttgc tgttgttcca
     4021 cctaaatcaa tcccaataat cttcttgtcc attacaaatt tcctttcttt cggctttctt
     4081 caattcggtg ctctcttttt aatatgcgcc acgctgtcat gtatgtatca tgatcaatca
     4141 aacggctatc atacagatta cgcaattcga tagtcattaa ttcaatgtca tagatgcgtg
     4201 cacccacata gataaagatg ccaaattttt tgaacagctg ctgtacatca tataagctct
     4261 ccatctttaa gcacttcctt catattatac aagtaaacca tattttttta aacccagtaa
     4321 aatacatatt acaaaaagaa atacaaaaac tagtgtagaa agaatccttt ggtgtatcga
     4381 atagcttgtc tgttgtttcg gtagacccaa aacattccct aacactaatc cgccaattag
     4441 cccaccgaca tgtccccaaa catctacaga ggtatccaga agaccaaaaa caaatgtcaa
     4501 cacaataaat aaaagaaaat gtctgactag ctgctggatc gctgggttat gtttaaaatg
     4561 aaaccctaag ataaacaacg caccgaacaa accgaatatc gaagtactcg caccaccaga
     4621 cagaacattc gcttcgttaa aagcaaagct tgctgtattt cccattatcc cgctgaacat
     4681 ataaatcaag aaaaaacgcc aatggccata gattgcctcg atttgctgcc ccataaaata
     4741 cagtatcagt gagttcatta cgaaatgcat cagtccaaaa tggatgaaga ttggtgtcac
     4801 taaacgccac cattcattga agtgaacaag ataaggacca tacattccgg cataataagg
     4861 gatctcaagt cgcggaaata attccatcat gaggaagacc gctgtctgta tccctaaaaa
     4921 agcataggtc cataacggtc gtcttaacca tgcacgcaat tttatttgct gttgataatt
     4981 catgaaaacc tcccatttca ttttagtcaa taaacaattg ttggatcttc tgatcaaagc
     5041 tctccggttg ccattgttca ttcaattgtt cacgaaaaac aaggctacat gtttttttat
     5101 gaaaattatt taaataccgg tcgtaataac caccgccaaa accgattcga taaccggctc
     5161 tattgtagat aatacctggt acaatgagga gatcgatcat ctccggcaaa tagagcttct
     5221 ctgattgtgg ctcaagaaca ccaaaactcg aacgtacaag aacggactgt tcatccattt
     5281 cataaaacgc catctgacgt ttcggcaaag ttttaggtac aactattttc ttttgttctt
     5341 tttctgctcg ttggataacc ggctgtgtat ccagttcaaa ttcattcgat aaagtcgtcc
     5401 ctatcacttt tgctgacttc cagtactcgc tggcgaatag ctgtttatag attcgctgtt
     5461 ctttttcctt tttcttgcca cttttggcca gatttttcag cttttcgatt cctttccggc
     5521 gtaatcttac tttcaattgg tccaaaaact cacccttcct tctctcataa aatggtatag
     5581 gaaaaaaaga aaaatagcaa tcgaactttc aaataaatgc ataaatagaa taaaaagaag
     5641 acctagaaat gattggcctt ccttttttaa taaatttcaa ctgttcagcg cgtgtttttg
     5701 tctgataaaa agtgcataca gaaaatatgg cgcaccaaaa agggcaatca aaattcctgt
     5761 aggtacttcc ccacttggta gaatcactct agcaaccata tctgcactaa gcagaaaaat
     5821 ggaaccgaac aaaccgctca gaacaaaggt ccagttattt tctttacgta cgactagctt
     5881 cgcagcatga ggagctagta gtcctacaaa agataaacta cctgtcaccg ctacactacc
     5941 gcttgccaaa gcaacagcta acaacaaaag gattttttga cttttagcca cgttaaggcc
     6001 taatccttgc gcgcgttctt gaccaaatga tagcaatcca atcctctgtg cataaagtcc
     6061 tcctagcgga attaagataa ccgcccatgg aagcaatgcc caaacataag gccagctcgc
     6121 accccagatt gatcccgcta accatgcatt cacaaatcca taattttcct tagataattg
     6181 gatcgtgcat agcaatgtta atgcagaaag gccggcatta acggcaatac cggacaataa
     6241 gattcgattc atacccaaaa agccatttcg atcataggcc gcaagataga cacagcaagc
     6301 agctaaaaca ccacctagaa aggctacaac tagcaataga aacggcagcg aatagtgcga
     6361 aaagaaacct aaataaagca tcactaaaaa tcctgctccg gcattaatgc ccaatatact
     6421 agaatctgcc aattcatttc ttgttactcc ttgcaagata taaccagaaa cgccaagaca
     6481 agctcctgct aataaactga tcagtaatcg aggaactcga aattctctaa caatcagcgc
     6541 caaagaagat gattctttcc ctatgaacgt ggcaaaaagc tgatccaggt aaataaaagg
     6601 cgtaccaatc attaaactac ctatagacag gatgacaagt acaaaaagca agaaatagaa
     6661 cgtccaatat atttttttca ccgttgttcc ctccttactt gtatcagtaa aaacggaacg
     6721 ccaatcaatg caatgactaa tccaaaaggt gtttcaaaag ggggattgat catcctcgct
     6781 gtaaaatccg ccaacataac caatccaccg ccaactaaaa aagagacagg tagaacataa
     6841 cgatagtccg ttccaaccat tccgcgaaca actggcggaa ccaacagccc aataaatgaa
     6901 acaggtccga ctaacgaaac ggcaatggct gctaacactg caactagaca gaaaacaatc
     6961 aaccgcgttc gtttaggatg acttccaaga gctaatgcat gtgtttcact caaatgaatc
     7021 aaagatattg cttttcctaa tgaaaaagaa accaaaacag caagtatata taaaggaaac
     7081 aatactgcta attgtttcca tgtgatattg gcagctccgc ctacaaacca aaaagcaaga
     7141 tcctgctgca gattaaagaa ttgggtaagt gcttggctga tagcagtaaa aaaagaacta
     7201 atagctacgc cagctaaaat cagctgtaaa ggattcatgc ccattttcga aaactgcaaa
     7261 aaaccaaata cgagcaaagt tacagcaaaa gaacctgcca atgacattag aaacacagaa
     7321 aaatcagaag ctttaggcca aaaagcaaaa ctaagggcca ttgccaacga agcacccgcg
     7381 ttgattccta gtaagcctga atctgctaaa ggatttcttg tcactccttg catcaatgca
     7441 ccgctcaatg caaaacaact ccctacaaga aatgctcccg ccacacgagg caaacgaata
     7501 tttcgtatga tttggtgact ttgttttgat ctatcaaaat caaacaacgc ctgccaaaaa
     7561 tctgcttttc ccacagtcgc tgcgccattt gaaagagcaa caaaaaaaat aatcaaaaga
     7621 acaaaacaga ccaaaagcag gagtctattt ttcttcatag tcctcataat tgatcctcct
     7681 gctcttttgg caacaaatca taggttagaa aaactggttt atttgttctt ggaagcgtga
     7741 caatcgttgc atcaatctca aatatttttt tcatacactc ttttgtgatc acttcttcag
     7801 gtgtaccttc tgtgatcaac ttaccgtttt tcattgctaa taaataatct gaaaaacgtg
     7861 aagcatgatt aatgtcatga atcgacatca agatcgttgt ttgtttcatt tgattgattt
     7921 cagtcaataa ctgtaggatc tctaattggt gtgccggatc taaaaatgtt gtcggttcat
     7981 ccaagatcaa gatatctgtt tcctgagcaa gcgccatagc gatccagaca cgctgggttt
     8041 gtccaccaga cagcgttgcg atatcccgca tacgaaatcc agctagacct gtagcatcca
     8101 acgcccactc gattgcttca tgatcttgcg tgtccatgcc actgaacagt ttttgataag
     8161 ggtatcgtcc gtaagatact acttcttcta ctgtcatacc tgtagggtgt tcactagttt
     8221 gtgctaataa tgtaattttt tttgcaagtt cttttgaact gtatgtagag atatcagagt
     8281 tattgacaat gatctttcct gatttcacag gtagtatacg ggtaaaactt ttcaataaag
     8341 tagattttcc acttccattt ggcccaatca aactagtgat tttcccttca ggaatcgtaa
     8401 cagacatgtt ttcaatgatt tttttgtttt cgtagccggc tgcaatgtca gctgctctta
     8461 atctcgtcat ttcacccatc ctttcgctga aaatcattct caattatgac gctgtttcaa
     8521 catttcgtca agcgctactt tcatatctat agccaaatga agcggttgac agcgttcgaa
     8581 atatcaggaa taataaccaa tgagaaagat tctcaattat gttaggagga aagaaatgaa
     8641 attaaaaaca aaagtattca cattatctag catattgctg ctggctggag tactgagcgc
     8701 ttgctccccc tcttcagata aagaaacagc tacaactgcc aattcgacga cttcatccaa
     8761 tagtactcat acagtcacag atacacttgg acataaagta gagattccaa acgcgcccaa
     8821 acggatcatc ggcagttact tagaagacta tctgattgca ctaggggaaa aacctatcgc
     8881 ccagtggact gttggcagtg gttccattca agattatctg caagatgatt taaaggatgt
     8941 cccaacgatt tcttatgacc ttccttatga gaaagtgtta agctttgagc ctgatttgct
     9001 gttgatttct tcttccgcta ctgtcgaagg cgggaaatac gaacaatata gcaaaatcgc
     9061 gccaacttat gttgtaaaaa acggaacaga tgtcacatgg gaagaacaac taaaagatgt
     9121 cggcaaagct ttggcaaaag aaaaagaggc agaaaaaatc atttccgatt atcaaaaatc
     9181 catcaagaaa actcgagaag aactagcaga taaaatcgag aacaagacag ctgcagtgtt
     9241 gtgggtgacc aacaactcag cctttatggt cagcaaaaat cggtcaagtg gaagaatcgt
     9301 atacgacgat ctggaatttg gtgttcctga tctagtggaa gaggtctcca aaaaagccac
     9361 ttctgactgg tctgctgttt cctctgaaaa gctagctgaa ttagatgctg actacatcat
     9421 ccttgtgaac agtgacaaag atgctgccat gttcaatgaa ccaagctgga agaatcttaa
     9481 agcagtgaca gacaatcatg tactgcaatt cggacctgaa tctagttggc tatataacgg
     9541 acctattgcc agtcaggaaa tggtcaatga tatcaaagaa aaactaaatt gattttaact
     9601 aaaaaaagaa tcttcacgat tagtcgtgag atgctttttt tcttattaac aaactagacg
     9661 catagatttt attcttcttt tgaagcaacc ctatttttta ctctttccca tttttgaaaa
     9721 cgatgggcat atagattttc ttgatccgtt atcccctcat actcctctat aagggcgaag
     9781 tctgaaaaat caatattccc aatatacgta tcgccttcaa acgtgctatc gatcaatgtt
     9841 cgccagatcc gaccagtgta aggaagaagt tcttgaaaaa tccgactacc acccgaaaca
     9901 tagatatctt ctgagatcgt ttgactatac gacaaaagtt catcgatcga atgcattact
     9961 tccgcatttt ctttcgtgcg aaaatttggt tgcgttgtca acacgatgat atggcgataa
    10021 ggaagtgaaa gatcccccat tccttcatag gtctttcttc ccatcactaa aattcgatcc
    10081 attgtatgtt cgcgaaagaa acgcatatca tttggcaatc gccatggtaa caaaccattt
    10141 ttgccgatca gaccattttt atcttgtgcc cacatggaaa taaacatatt atccgctcct
    10201 tatgatttta gatgttttga gataaaaaaa gtctcaattc tttgaccagc aaggaattga
    10261 gaactttaaa aacattattt tgtttcacgg tgcaaagtaa ctttgcgttc gcgtgggcaa
    10321 tatttttgtt tttccaaacg atcaggattg ttacgtttgt ttttgcttgt tagatagtta
    10381 cgttctttac aagaagtaca ctctaaagtg atgtttacgc gcatgttttt ccctcccatt
    10441 tttacaagaa tcctctaaga tgactctcag acttgattat cttaccatga ttttttctag
    10501 attgctagtg tttttcacga ttttgttaag taaaactact tgacgataaa tacaacggtt
    10561 ttacttctca aacaaaatct ttttcttttt gtataaatac ccttacactt gtttatatag
    10621 att
//
LOCUS       DAZBTF010000089        10061 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000089 DAZBTF010000000
VERSION     DAZBTF010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.090"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            91..765
                     /locus_tag="ACHGNS_002491"
                     /pseudo
     CDS             91..765
                     /locus_tag="ACHGNS_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            758..919
                     /locus_tag="ACHGNS_002492"
     CDS             758..919
                     /locus_tag="ACHGNS_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938387.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
     gene            1403..1999
                     /locus_tag="ACHGNS_002493"
     CDS             1403..1999
                     /locus_tag="ACHGNS_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFN6938388.1"
                     /translation="MNPTLITKKELLKKLDISTGVLANLIRNGMPKEGEMFNLDKIIT
                     WRENWSKNILGELEVGRVYTNKEISEKFKCSKQGGMRRSHQTNTLVLFSDQTGSNVYK
                     DKWLNGILQYTGMGLKGDQVLDKNQNKVLANSKSNFVKIHLFETFKPKEHTYLGEVYL
                     AGQIYTVNEKDSSGNSRKVYKFPLALINQEQLIEDKDI"
     gene            1980..2426
                     /locus_tag="ACHGNS_002494"
     CDS             1980..2426
                     /locus_tag="ACHGNS_002494"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFN6938389.1"
                     /translation="MKIKIYNQEENQTRHIRNLSNAKLEEEARKVSNYNKKMAAKYVN
                     EEEFCFSKTKIYKRNPFVSEYVKRLAKGICQLCQEKGPFIKDGVPYLHCHHIEYLSQG
                     GKDVIENCIALCPNCHARIHELELPDDKEKLLKIVEKRENSFSKIP"
     gene            2769..3092
                     /gene="relB"
                     /locus_tag="ACHGNS_002495"
     CDS             2769..3092
                     /gene="relB"
                     /locus_tag="ACHGNS_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFN6938390.1"
                     /translation="MRFFKRGNYRAMITVRVSDAKKEWLSYMAEFYGITLSELLKNYS
                     MEQLEDEYNRQTAEIAHKHWIRSGKTTVFMEESLMNLVGWNDLPFKIHARNSEAALIG
                     KYVYW"
     gene            3412..3639
                     /locus_tag="ACHGNS_002496"
     CDS             3412..3639
                     /locus_tag="ACHGNS_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFN6938391.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            3655..4821
                     /gene="hemW"
                     /locus_tag="ACHGNS_002497"
     CDS             3655..4821
                     /gene="hemW"
                     /locus_tag="ACHGNS_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFN6938392.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            5056..6099
                     /gene="hrcA"
                     /locus_tag="ACHGNS_002498"
     CDS             5056..6099
                     /gene="hrcA"
                     /locus_tag="ACHGNS_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFN6938393.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            6193..6756
                     /gene="grpE"
                     /locus_tag="ACHGNS_002499"
     CDS             6193..6756
                     /gene="grpE"
                     /locus_tag="ACHGNS_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFN6938394.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            6807..8636
                     /gene="dnaK"
                     /locus_tag="ACHGNS_002500"
     CDS             6807..8636
                     /gene="dnaK"
                     /locus_tag="ACHGNS_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFN6938395.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            8787..9953
                     /gene="dnaJ"
                     /locus_tag="ACHGNS_002501"
     CDS             8787..9953
                     /gene="dnaJ"
                     /locus_tag="ACHGNS_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFN6938396.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT         3556 a         1596 c         2110 g         2799 t
ORIGIN      
        1 aatgttaata tggttaaaat gaccgttata ttttttatat aaatatcgta taattagtat
       61 gaatatagaa gaaggaaaga aggtgcaatc ttggaaccta aagacaagta tttagattac
      121 gctagggtca ataaagataa atttattgaa acaatcatca agggaaaaat ccctgatgat
      181 gaaaaagatg cggtattcat ggctggaagc ccaggagcag gtaaaacaga ggttgcacta
      241 ggtttagctt aaaattatga caaccatgtt gtgattgatg cggactattt tagagcacaa
      301 tttccagaat acaatggaaa gaactcaagt gtttttcaga aagcttcttc ttggttagtt
      361 gaacaatcat taaaatatgt actggaacat ggctattctt tcatactaga ggatactttt
      421 gctatcctta gtgcagagaa aaatattatt cgagccctta aaaataactt tcgcgttacg
      481 attttctatg tttaccaaga tcccaaagtt gcctgggatt tcactcgaaa acgtgaacta
      541 gctgaaggac gtcatgtgcc aaaagaaatg tttatcaatg cgttttttaa gtccagagag
      601 aatattgaaa aagtcaaaaa tagacatcct gaagtagtcc ttcacataat gattaaggat
      661 tatcaaaacg acatttctga agtgcattat gcagcagata atattcaatt agtactacca
      721 atacaatata cttcaaaaga gttggaggag gaattacatg actgagaaag aacaagtaca
      781 acaaatcgtg aagaagtata ataaaagtat cgctgattta tcagaaaacg cctcagcgaa
      841 agaatttaaa acagtaatga attacgtagc agatgaagca aatcgtaagc agcgaaaact
      901 agttgggttg gataaataaa ttttttaaac catatccttg taaagttgag ttttttattt
      961 taatgaatta agactattaa atagataatt attgtaactt aaaaaacaca gctaaaaatt
     1021 agttgtgttt tttattaggt ttacttttct ggtataaaag gaactagcta ctttttcaaa
     1081 atttctaaat cattcaatat gtagcgctta ctaaattatt cgccataaaa ttttataatt
     1141 ggatcactca ttgttttacc taagtcatat ttaagtaata accggtgttt ttacataaga
     1201 agacttcggg tattttttag tttgattagc tctgatatac ctataaagaa tatgctaagg
     1261 taagaatagt aaaaattcac atagatgagg tataataatg atatttttat atattacaga
     1321 taacttgaca caaaatgagt ttttttcgat ttttcaagtt atctaatgca attatattga
     1381 tatcattagg agctgcattg ttatgaatcc aacattgatt actaagaaag aattactaaa
     1441 aaaattagat atttctactg gtgtattagc taatttaatt agaaatggca tgccaaaaga
     1501 gggagaaatg tttaatcttg ataagattat tacatggcga gaaaattggt ctaaaaacat
     1561 attaggtgag ttagaagttg gtagagtata tactaacaaa gaaatttcag agaagtttaa
     1621 atgttccaaa cagggtggaa tgagaagatc tcatcaaact aatactcttg tactgtttag
     1681 tgatcaaaca ggaagtaatg tttataaaga taagtggtta aatggtattt tacaatatac
     1741 cggtatgggg ctaaaagggg atcaagtatt ggataagaat caaaataaag ttttagccaa
     1801 ttcaaaaagt aattttgtaa agattcattt atttgaaaca tttaaaccaa aagaacacac
     1861 atatctggga gaagtatatt tagcaggaca aatatatact gttaatgaaa aagatagttc
     1921 gggcaacagc agaaaagtat ataaatttcc attagcttta ataaatcaag agcaattgat
     1981 tgaagataaa gatatataac caggaagaaa atcaaactag acatattcgt aatttatcta
     2041 atgctaaatt agaggaagaa gcaagaaaag ttagtaatta taataaaaaa atggctgcaa
     2101 aatatgttaa cgaagaggag ttttgcttct caaaaacaaa aatatataaa cgtaatccct
     2161 tcgtatctga atatgttaaa cgacttgcaa aaggtatttg tcaactttgc caagaaaaag
     2221 ggccatttat aaaagacggt gtaccttatt tacattgtca tcatatagaa tatttgtcgc
     2281 aaggtggaaa agatgtgatt gaaaactgta tcgcgttatg tcccaattgt catgctagaa
     2341 ttcatgaact tgagttacct gacgataaag aaaaattgct gaagatcgtt gaaaaaagag
     2401 aaaatagctt ttctaaaata ccgtaattta tttttatagg acaaataagc taatgtgagc
     2461 tatgccttct atataagact aagttctatt ataagtaacg aagtaattct cgttccttcc
     2521 tgtagtaata ttttttaaat aacctatagg cttcttcaaa agtacatcct ccagtaaatt
     2581 ctaagtactg gaggattctt tttctagtgt tgctttactc atgaaaaagt agttggctac
     2641 ttcatcaaaa tttctgaact tttttaatgt ataataaaga gctcgatcat ttactacaag
     2701 gatgctagct cctacatttt cacagttttt tagcaagtaa atttttctaa aatcacatca
     2761 ggaaatgcat gagattcttt aagagaggca attatagggc tatgattacg gttcgtgtaa
     2821 gtgacgccaa aaaagaatgg ctttcttata tggctgaatt ttatgggatt actttatctg
     2881 aacttctaaa aaactattcg atggaacaac tagaagatga atacaatcgt caaacggctg
     2941 aaattgccca taaacattgg ataagaagtg ggaaaacaac agtattcatg gaagaatcct
     3001 taatgaattt ggtgggctgg aatgacctgc cgtttaaaat tcacgcccga aattcagaag
     3061 cagctctgat tggcaaatac gtgtattggt agagatagaa gataatcaat tcgttgttat
     3121 cgctattcaa gtagggcacc gaagaaatgt ctatgattaa gagactgttt atttttacaa
     3181 aaaaataata attggaatct agttaaaact cttgaagtga gagatttgac tagatttttt
     3241 tatgaataaa ataaggacaa aattgtttag gaagaatcat gagtgtaaaa aaatcatcca
     3301 agaagtcgat tcttggattt tttagtggat ttgttcgcca aatacgttta gtatgcgtat
     3361 atgagaggtt gtaagcaaag cgttgtggtc tgagcactta aggaggaatc tgtgaaaata
     3421 gcttttcata tttttgcaga ttttgactta atgccgaagg tcagctttgc gattaccgtt
     3481 tatcgagagg ctgtaaaagc accgtgtagc tccgatgaca ataagcaagc atttttagag
     3541 ctaggtttcc ttgattctaa atatgcggct tatttcaatg agttggtgct tgaacgcgat
     3601 tttaagaagg tcgaaaacag gcacgacttt tcgacgtaag gagaaagaat ttcaatgata
     3661 gaaacaataa atcccgcaga aaacaaggtt tctgcgtaca ttcatatccc attttgcgag
     3721 catatctgct attactgtga ctttaataaa gtttttttag aagggcagcc agtagatgag
     3781 tatgtggaaa tgctgctgaa tgaaatgaga ttaatgctgg aaagtcatcc tacagcgaat
     3841 ttggaaacac tttatgtagg aggcggaaca ccaacttctt tatctgctaa acaacttgat
     3901 cgactattat caggtgctag agagatcctg ccttttcaaa atggtaatga gtttactgta
     3961 gaagcgaatc ctggtgattt gacccgtgaa aaacttcaag tgatgaaaaa ctatggcgtc
     4021 aatcgtttat ccatgggagt gcagacattt gacaatcgct tattgaaaaa aattggacgt
     4081 aaacatactg cagaagatgt gtatcaaaca atgcaatttt tagaagcaga aaattttacc
     4141 aatgtaagca tcgatttgat ttatgcatta ccgggacaaa cactagaagg atatcgggat
     4201 actttggaaa aagctttggc tttggatctg cctcactatt ctttgtactc attgatcctc
     4261 gaaaacaaaa caatgttcat gaactgggta cgacaaggaa gactcgaact gcctgatcag
     4321 gaaacggaaa ccagaatgtt tgaagagacg attgaagcaa tggaaaagca tggacgtcac
     4381 caatatgaaa taagtaactt tgggctctcc ggacatgaat ctcaacataa cctcatgtac
     4441 tggaacaatg accactattt tgggtttgga gctggtgcta gcggttatct tggagatatt
     4501 cgttaccgca acaaaggacc aatccaacac tatttacgcc cattacgagc aaatgaacgc
     4561 ccggtattgg aagaagaaaa attgacacga aaaaatcaaa tagaagaaga aatgttctta
     4621 ggactaagaa aaaaaacagg gattactaaa aaacatttct atgatcgcta tcaaaaaaca
     4681 attgaatcgg tgtacggtga tgtgctaagg gaattagagc aagaagggtt acttgttaat
     4741 gaagaagacc gtgtttattt gacaccaaga gggacttttt tggggaacaa tgtgtttgag
     4801 cgctttttat ttgaaaaata gtttgaagaa tatttgtttt ttatcgaaac ttgaaaagaa
     4861 aaagtttaga aagtttgaca atgaggtatt gaagtattga tataactgga tttacatcaa
     4921 ttatttagaa ttagcactct tgtgtttaga gtgctaaaaa atggagtctt tctcttgaca
     4981 ggagaagggc ttcttgctat atttataagt gaattagcac ttagaatata tgagtgctaa
     5041 tgagaggtga ggaagatgtt aacaaagcga caaaacgaca ttttacgtct gatcatccaa
     5101 cactatacga gtagtggcgt gcccgttggt tccaaaacat taatgtctga gggagtcaag
     5161 gccagctcag caacgattcg caatgatatg aaagcattag aagatgaagg attgctgttg
     5221 aagactcact cttcttctgg gcggattcca tctgctttag gctatcgtta ttacgtggat
     5281 catttgttaa aacctgctcg cgtagcagaa gatgatttgc tgctgattcg tcagtctcta
     5341 ggcagagaat ttcatgaaat caatgagatt attaagcaat ctgctgagat attatctgag
     5401 ctgacaagct atacgacttt ttcattggga cctgaaataa aagaccgccg actgactggc
     5461 tttcgtatcg tacccttgaa taatcgacag attattgcta tcgtcgtaac agatatgggg
     5521 aatgtagaaa gccaagtgtt cactcttccc gaatatatgg gaagtcagga actagaaaaa
     5581 atggtacaga ttgtaaacga ccgtctagtc ggagatcctc tgatgacggt gtaccataaa
     5641 cttcgtacgg agattccgat gattcttcat aaatatttcc agacgacaga agggatgtct
     5701 cagttatttg acaccgttct tggtcatgtt tttgaagaaa aggtttatgt cagtggacaa
     5761 atgaatctct tggattatga gccccgtcaa gatcttgatc agttcaaatc gatgttttct
     5821 tttatgaaag actcagacgg acttacacaa atgatcactc cgatggatag caatattcat
     5881 atcaagattg gtgctgaatt aggcaatgat ttattgcaga atatgagtat gattcaagca
     5941 agttacgaga tagatggtca tggaagaggt acaatagcct tgttagggcc tactagtatg
     6001 ccttattctc ggatgttcgg tctagtcgat gtttatcgaa gagaactagc agctaaactt
     6061 gctgattact accgctcgct tgacttgtca aactcgtaat gtgaataacg aagctatttg
     6121 cagctgatcg ttctctcttt cttagtaaga gcaatcaaac agctgactga ataatgaaag
     6181 gaagactgta tcgtgaaaga aaatcaagaa gaactagata aagaaatgac agatgctcag
     6241 ccagaaccgg aaatcgatgt cgaagctgca gaagattcgg gtatttcaga agctgaagcg
     6301 gaagaattcg aaacagcgaa attaaaagct gagttagaag aaatggaaga taaatacttg
     6361 cgggctcgtg ctgaaatcgc aaatatggca aatcgaggaa aaaatgagcg tgagcaatta
     6421 caaaaatacc gttcacaaga tttagccaaa aaattacttc catcaattga caacctggaa
     6481 cgtgcattag ccacagaagt gtcagatgat caaggggcag gtttgaaaaa aggcgtggaa
     6541 atggtcttgg aaagtttgcg taacgcactg gaagaagaag gaattgaaaa aatccctgct
     6601 aaaggagaag cctttgatcc taatcttcat caagcagtac aaactgttcc agcgactgaa
     6661 gatacaccag ccgatacgat tgtagaagta ctacaagaag gctacaaatt acatgatcgc
     6721 gtgttaagac caactatggt cattgttgca caataattgt gcggatataa aacttacact
     6781 ttcaattaaa gaaggagacg ttaattatga gtaaaattat cggtattgac ttaggtacga
     6841 ctaactctgc agtagcagta ttggaaggaa acgaagcaaa aatcatcgct aacccagaag
     6901 gaaaccgtac aacaccttct gttgtatcat tcaaaaatgg agaaatccaa gtaggggaag
     6961 ttgcaaaacg tcaagctgtg acaaacccaa atacgatttc atcaatcaaa cgccatatcg
     7021 gtgaagcagg ttacaaagta gatgtagaag gtaaatcata tacaccacaa gaaatctcag
     7081 caatgatcct tcaatatatc aaaggattcg cagaagacta tttaggtgaa aaagtcgaaa
     7141 aagcagttat cactgtgcca gcttacttta atgatgcaca acgtcaagca acaaaagatg
     7201 ctggtaagat cgctggtcta gaagtagaac gtatcgtcaa tgaaccaact gcagctgctc
     7261 ttgcctatgg tttagataaa acagatcgcg acgaaaaaat cttagtattc gaccttggtg
     7321 gcggtacttt tgacgtatct atccttgaac taggagacgg tgtctttgat gtattgtcaa
     7381 cagctggcga caaccatcta ggtggggacg actttgataa caaaatcatc gactacatgg
     7441 tagcagaatt caaaaaagaa aacagcattg acttgtctca agacaaaatg gctttacaac
     7501 gcttgaaaga cgctgctgaa aaagcgaaaa aagatttatc tggcgtatca agcacacaaa
     7561 tcagcttgcc atttatcaca gcaggagaat ctggaccatt gcatttggaa atgacattga
     7621 cacgtgcaaa atttgatgaa ttaacagctg acttagtaga acgtacaaaa gtaccagtac
     7681 gtcaagcgtt aaaagatgca ggtctgtcac aatcagaaat cgacgaagtt attttagtcg
     7741 gtggatctac tcgtatccct gcagttgtag aagcagtacg taaagaaaca ggaaaagaac
     7801 caaacaaatc agttaaccct gatgaagtag ttgctatggg tgctgctatc caaggtggcg
     7861 tgatcactgg tgatgtgaaa gatgttgttt tacttgacgt tacaccattg tcattaggta
     7921 tcgaaacaat gggtggagtg ttcactaaat tgattgaccg caatacaacg attccaacaa
     7981 gtaaatcaca agtattctca actgcggcag ataatcaacc agcagtagac atccatgtat
     8041 tacaaggtga acgcccaatg gctgctgaca acaaaactct aggaagattc caattgacag
     8101 atattccagc agctcctcgt ggtattccac aaatcgaagt aacattcgat attgataaaa
     8161 acgggattgt aaatgtatct gcgaaagatc taggtacaca aaaagaacaa aaaatcacga
     8221 tcaaatcttc ttcaggtcta acagacgaag aaatcgaacg tatggtgaaa gatgcagaag
     8281 caaacgctga agcagataaa gctcgtaaag aggaagttga cctacgtaat gatgtagacg
     8341 cattgctatt ctctgtggac aaaacattga aagaattaga aggcaaagtg gacgaagaag
     8401 aagtgaaaaa agcagaaaca gctcgtgacg aattaaaagc cgctgttgaa gcaaacaaca
     8461 tcgaggaaat gaaaactaaa cgcgatgcat tgaacgaaat cgttcaaaac ttgactgtta
     8521 aactatacga acaagctgca caacaacaag ctcaagaaaa tccagaagca gctcaaggcg
     8581 gagcagatga tgtcgttgat gctgattttg aagaagtcga tggcgacgat aaataatcag
     8641 ttcactgaat ttcgagagaa ggccaaagtt gctactttgg ctttttcttt tgaaaaatag
     8701 tttgaagtat cggtttttgt ttcaaacaaa atatgatatg ataacaagcg acgtgaaaat
     8761 gatgagaacc aataggagga aagcctatgg caacaaaacg tgattattat gaagtcttag
     8821 ggctctcaaa aggagcatca gaagatgaaa taaagaaagc ttatcgtaag ctttcgaaaa
     8881 aataccatcc tgacatcaat aaagaacctg atgcggaaga aaaattcaaa gaagtatcag
     8941 aagcatatga gattttaagt gacccgcaaa aacgcgctgc atacgaccaa tacgggcatg
     9001 caggcacaga tccaaattac ggtgctggtg gagctggtgg cggatttggt ggattcggcg
     9061 gcttctctgg tggcggcttc ggtggttttg aagatatctt cgactctttc tttggaggag
     9121 gcggtcggtc agtcgatcca aatgcgcctc gtcaaggtgc tgatttgcaa tatacggtcg
     9181 acttgtcttt tgaagaagcg atttttggtg tagaaaaaga aatcaagtat aatcgggaag
     9241 aaatctgtca tacttgtggt ggaaacggtg caaaaccagg cacacaacca actacttgtc
     9301 ataaatgtca tggtgcaggt acgatcaatg tagaacgcca aacgccactt ggacgagtga
     9361 tgagccgtca aacttgtgat gtttgtcatg gcacagggaa agaaatcaaa gaaccttgtc
     9421 caacctgtca tggctcagga catgagaaga aagcacattc agttaaagtg aatgtaccag
     9481 ctggtgtaga agaaggacaa caaatgcgtt tagctggtca aggagaagct ggagaaaacg
     9541 gcggaccatt tggtgatttg tatgtggttt ttcgtgtaga agaaagtgat atctttgatc
     9601 gtgaaggttc tgaaatttac tatgaattgc ctttaagttt tgtacaagca gctttggggg
     9661 atgaagtcca agtaccaact gtccatggag atgtgaaatt gaagatccct gctggaacac
     9721 agacaggtac gaacttccgt ttacgtggaa aaggcgctcc gaagttacgt ggtggtgcaa
     9781 caggcgacca acatgtcaaa gtaaaactga tcacaccgaa aaatctaaat gaacaacaaa
     9841 gagaagcatt acgtgcattc gctgaagctg gcggcatcaa agttgaagaa cagcaagaag
     9901 acggtttctt cgataagttt aaagatgctt ttggcgggaa aaagcggaag tagtttttct
     9961 agtaaaaata taaagtagac ttgcccaaat tttctatttc tagtaaaaac gctaagtcat
    10021 taataattaa aaaccagacg aacaatcata cgttcgcctg g
//
LOCUS       DAZBTF010000090         9940 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000090 DAZBTF010000000
VERSION     DAZBTF010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.091"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            32..388
                     /locus_tag="ACHGNS_002502"
     CDS             32..388
                     /locus_tag="ACHGNS_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich SFCGS family protein"
                     /protein_id="HFN6938397.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            404..754
                     /locus_tag="ACHGNS_002503"
     CDS             404..754
                     /locus_tag="ACHGNS_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HFN6938398.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            783..1562
                     /locus_tag="ACHGNS_002504"
     CDS             783..1562
                     /locus_tag="ACHGNS_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HFN6938399.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            1573..2235
                     /locus_tag="ACHGNS_002505"
     CDS             1573..2235
                     /locus_tag="ACHGNS_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HFN6938400.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAIFPA
                     VN"
     gene            2254..3360
                     /locus_tag="ACHGNS_002506"
     CDS             2254..3360
                     /locus_tag="ACHGNS_002506"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HFN6938401.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            3350..4441
                     /locus_tag="ACHGNS_002507"
     CDS             3350..4441
                     /locus_tag="ACHGNS_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HFN6938402.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            4454..5194
                     /locus_tag="ACHGNS_002508"
     CDS             4454..5194
                     /locus_tag="ACHGNS_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HFN6938403.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
     gene            5482..7422
                     /locus_tag="ACHGNS_002509"
     CDS             5482..7422
                     /locus_tag="ACHGNS_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFN6938404.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            7509..9431
                     /locus_tag="ACHGNS_002510"
     CDS             7509..9431
                     /locus_tag="ACHGNS_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFN6938405.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
     gene            complement(9711..>9940)
                     /locus_tag="ACHGNS_002511"
     CDS             complement(9711..>9940)
                     /locus_tag="ACHGNS_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HFN6938406.1"
                     /translation="IDRDYFTQADLIITLCGDAKDKCPAVPNNHEHLHWDLKDPAQAV
                     GSEEEILTEFRKVREGIKRKVLDLNQRTSIV"
BASE COUNT         3245 a         1650 c         2207 g         2838 t
ORIGIN      
        1 acaaatgata ttttacgagg aggaaacaat catgattaaa gtagttattg cagaccgtat
       61 gggtaaagga caaaatgtgg ccaaaggggt agaagcagct ggtggaaaag cagttgtcgt
      121 accaggaatg ggggcagata tgcgtttggg cgatgtgatg caacaagagc atgcagatat
      181 gggtatctct ttttgtggta gtggtggtgc aggtgcatta acagcagcaa caaaatatgg
      241 ctatccagaa cgacatggta tgcgttcgat tgaagaaggg atcacagcta tcaaggatgg
      301 caaaactgtt ctaggtttcg gctttatgga tcaagaggaa ttagggaaac gtctgacaga
      361 agaatttatc aaaaagcatg gaaaatagaa agagggacat gaaatgagcg ttcaaaagac
      421 aattcagcaa aaagttatcg tctccggtaa aggggaaaca aagcaacatg cttttgcatc
      481 agcgttagca gaaattcaaa acaaaataat ggaacaatca aacgtattat tgcgaatcga
      541 accagtcaat gtggatattt taaaggcggt cgaaaacaaa tacacagaac gctttctatt
      601 tttcttcttc ccacggcaac gtattagcta tgaagtcgaa ctctctgtta ctgtggctgt
      661 aacagaaata aaattggcag acatatcgtt cagccagcaa atgactgaag atccaaatgg
      721 gattgccatt ccatttgtaa ctaaaaaaat ctaattttga atcataaggg ggaaagaata
      781 acgtggattt cgtaatcatt ctagtcaaat cggtcattat cgggggatta cttggttttg
      841 cagcatctgt cggggcagct cgaatgtttc atgcacctaa tacacaaggt ttaggagctt
      901 ttagaacatt aggcgaaatg aatgcctgca tgggtgatcc agcttctcat ttttcatttg
      961 gtttaggctt tttctttaat gcatgggctt cttcagttgg tgctggtgca ttcacacaag
     1021 atgtgacgca tcgaatcatt cccaattggg cagcggcagc acttctagca aaaaacaaag
     1081 atgtcaccca gaccatgcat gatccgaaaa aaatggggat cattggagcg gttatcgggg
     1141 cagtggtggt agccttctta aacacaacag ctgcagcaat tccagaggcg ttacaagtga
     1201 cggcggtttc cgttttggta ccagcagcga cgattctgat caatacagta atgccagtta
     1261 ttttttggtt ggcagcttta gacgcaggga aacgaactgg tttttggggc actctttttg
     1321 gtggtatcgc gcaattgatt atgggaaatg cggtacctgg tgttgttcta ggaatcttga
     1381 ttggtaaagg cgtcgatgag agcggttgga atcgcgtcac gaagagcatg atgatcgcta
     1441 ttgttttatt atttattttc agtggatttt tccgtggatt tgatatggaa atgatcaaga
     1501 gcttccagct tcaagtgcct aattggcttg ataacttgca caacatcttt agtgtgaaat
     1561 aggaggagaa tcatggagac aacagaagaa caagcaaaag tattagaagc aaagcggcaa
     1621 aagagctttt ggtttgcaga ctggtctttt ccgctaatcg taggaattat gtccgctgct
     1681 gtatttgcag gaacacacat gtatgtgatg tatggtgtgg gtgcattcaa tgaagtttcg
     1741 atcgtggcga tgttaaaagc cgggctggat ggcggatcct atggcgcagc ggcagcgttt
     1801 ggtgctagtt tcttatttgc cagaatttta gaaggctctt tagttgggat cttggactta
     1861 ggcggttcga tcctaactgg gatcggaatt ggggtaccag caattctctt aagtatgaaa
     1921 atagcggcac caatcgataa ttttgcactc tctttattga ctggtttggt tctaggtttg
     1981 attgttggtg gcatcattgt cttgattcgt aaatttacga tcaatcaatc aaattccact
     2041 tttggtgctg acgtcatgat gggggcagga aattcatctg gacgtttttt gggtccatta
     2101 attatattaa gcgcgtgtgc agcatctatt ccaatcggca ttggcgctac attaggagca
     2161 ctaggattct atgcttggaa aaaaccaatt gctggaggtg ctattttagg agcaatcttc
     2221 ccggcagtaa attaatagaa agagggtcat taaatgtttg atactttgat taaaaatgga
     2281 aggttagttg atggatctaa aatagaggtc gctatagaaa aaggcatgat taaagcagtt
     2341 gcagcagaaa taaatcagcc ggcgcaagaa gtagttgact tggagggaaa gtattattta
     2401 tccgctgggt ggatcgatga tcatgtccac tgctatgaaa agatgaatct ttattatgac
     2461 tatcctgatc agataggggt cgaaaaaggt gtgacgacgg tcattgatgc tggaactacg
     2521 ggagcagaaa acatccgtga attttatcaa ttgacgaaat cggtcaagac aaatgtgtat
     2581 gcgctaatga atatctctaa atggggcatt gtcaaacaag atgaacttgc tgatctaaca
     2641 aagattcaag aagatctagt aagtactgcg ttagcagaac tgccggaatt tattgtcggg
     2701 attaaagcga gaatgagtaa gacagttgtt ggtgaaaatg gcattaagcc attagaatta
     2761 gctaaaaaaa tccagacaga aaacaatgat ctgccattga tggttcatat cggttctgca
     2821 cctcctgagt tagaagaagt attgtcaaga atggaaagag gcgatgtatt gacccattgt
     2881 tttaatggaa aaccaaacgg tattttagat ccaaagacag atcaaattaa aaactttgtc
     2941 tgggatgctt acaataatgg tgtcgtcttt gatattggac atgggacgga tagctttaat
     3001 ttccatgtgg cagaaaccgc actaaaagca ggaatgaaag ctacctctat tagtacggat
     3061 atctacatcc gcaatcgtac aaatggccca gtttttgatc tagcaactac tttagaaaaa
     3121 ttgagggtag tcggctattc ttgggaagaa atcattgaaa aagttacagc agttcctgca
     3181 cggaattttc atttgaagaa taagggccag ttagctgtca attatgatgc agatttgacg
     3241 atatttgaga ttgttgaaaa tgaaaaggaa ttgaaagatt ccaacggatt tacacgtata
     3301 gctaaagaac aaattttacc gattaaaaca attattggag gcgtcatata tgacaattag
     3361 ctatggaaag tttgatttaa aagaagtcat taatgcatcg ggaaaaatga cgatcctagg
     3421 ggtttctaag gtatctgaaa gtgtcctagc agcacaacga tttggaggcg agcatttttt
     3481 tgagatgagt gatcttgcca tcaaaaccgg cgcttatctt tctcaattac tgaaagtgga
     3541 agatgcgcaa atcgtttctt ctgcttcagc agggattgct caatctgttg ctgctttaat
     3601 cggacagggc gacatgtacc atgtctatca tccctataca gaaaaaatcg ctagacggga
     3661 aatcatcata cctaagggac ataatgtaga ttatgggaca ccggttgaag taatggttgc
     3721 tcaaggtgga ggaaaagtca tagaagctgg ctatgcaaat atgtgtacac cggaacatgt
     3781 ttccatgatg attacggaaa aaactgctgc gcttctatat attaaaagtc atcatgcagt
     3841 tcagaaaagt atgctgacgg tggcagaaat ggtagaagtt gccaaagctc atcgtttgcc
     3901 attgatcgtt gatgcagctg cggaagaaga tttattcaaa tatagcgaaa tgggcgttga
     3961 cttagtgatt tactctgggg caaaagcaat cgaaggtccg agtgccggac tggtcatagg
     4021 aaaaaagaag tatattcaat ggattcggct acagtctaaa ggtattggcc gagcaatgaa
     4081 aatcggtaaa gataatattt taggctttac ccaggcacta gaagattatt taaaaaatgg
     4141 gagcgaatcc ggtgactcca tgaagcaacg attagcgcct tttgttaacg caatcaatca
     4201 acttcctgac ctttcagcga aaatagttca agatggtgcc ggcagagata tttatcgagc
     4261 aagtgtaacg attaatggcg ataaaagtgc gaaagaagtc attcaggaat taaaagcacg
     4321 aaatccagca gtctacaccc gagaatatca agcaaataac ggaatcattg agttcgacat
     4381 tcgttccgtc agccaaaacg aaatggaaaa aattataaaa agattgagag aaatcatgta
     4441 ggaggctttt aaattgtcag ttatcccaaa ttattataaa aatcgtgtat gtttaaatgt
     4501 attagctcat tcagttgaaa atgcaaaagc atgctatgaa gcagcggaag ggcatgtcgt
     4561 actaggtgtg ctatcgaaaa attatgctag cgatgaagca gcaatcgaag atatgaaaag
     4621 gtaccaagac gcaaccaata atgcgttgtc agttggatta ggcgctggag atccaaacca
     4681 aagtcagatg gtttcgcgtt tatctggggt tttacaacca caacatgtca atcaagtatt
     4741 tactggcgtg gggacttctc gtgctttatt gggccaaaat gatacagtca tcaatggatt
     4801 ggtttcacca acaggtaaga tcggctatgt caatattgct acaggtccgt taagttcaca
     4861 agcgccagca gctgaagtgc ctattgaaac tgccattcaa atgctgcaag acatgggagg
     4921 tacttcaatc aagtatttcc cgatgaaagg cttagcacat agggaagaat ttaaagcagt
     4981 tgcggaagct tgcgcaaaat atgaattcta tttagagcca acaggaggaa ttgatctttc
     5041 aaactttgaa gaaattctga aaattgcaat cgatgcaggt gtaaaaaaag tgattcctca
     5101 tgtttatagt tcaattatag ataaagaaac aggagatacg cgtataggag acgtaaagat
     5161 actattaagc atggtaaaaa aaattattca ctaactcatt agaaaataag aagcttattc
     5221 cagaatgaaa ttaaatggaa taagcttttt ataattaatt acggaaattg ttaatgtaga
     5281 taataattta gatgtacgta agcagaagta cagaaatgta agattttcca atattatttt
     5341 attcaacata ataaatcgta tatcaatttt tttagcgaaa tttctattta ttccaagaat
     5401 ttcagttatt acggaatcat tttgtgctta ataaaaagaa accgttttta tatactataa
     5461 atgtagaaag gcggtaggaa aatgaaaaaa attattgcaa tcacatcctg tccagttggt
     5521 attgcccata catacatggc agcagaaaac ttagagaaag cggggaaagc agttggcgcc
     5581 gaggtaaaag tcgaaacaca tggttccatc ggtattgaaa atgaattgac tgcaagagat
     5641 atcgaagaag cagctggtgt aatcatcgca gcagatacta aaatagataa atcacgattc
     5701 ggcggtaaac ctttgataac cgtaggtgtg caagaaggaa tccaccatgc agatgagttg
     5761 gtgagagata ttttagcagg aaaagcgcca gtctataaaa gtacggaggc aattatttct
     5821 tccgaaaata aaagtgaatc agaaaattta ggtaaaaaaa tctataagag tttgatgaat
     5881 ggcgtttcgt acatggtacc tttcgtagtt acaggagggt tattgatcgc gatttcttta
     5941 acattaggag gaacagctac acctgaaggg atcaagatac cagaaggaac gatttgggca
     6001 acgatgaata gtatcggaag tatcgctatg gggttgatgg ttccaattct atctgcattt
     6061 attgcgcaaa gtattgcaga tcgtccagga cttgttcctg gatttgttgg tgggatgtta
     6121 gcggcaaacg gagcattata tggtagtgat gcgaatgcgg gattcttagg cgggatcatc
     6181 actggatttt tagcaggatt cattgcttta ggaatcaaga aagtgcgagt accgaaagct
     6241 ttacaatcga tcatgccaat tattgttatc ccaattttag gatcattggc agttggtttt
     6301 gtgtttatct atgttattgg agaaccagta gctcttctat ttagttcatt gactcacttt
     6361 ttagcaggga tgcaaggagg aagtgaaatt gttctagcta tgatcttagg agcgatgatt
     6421 gcttttgata tgggggggcc gttcaataaa gtagcctttt tgttcggatc tggcttgatt
     6481 gcagaaggca attattccat tatggggcca attgctgtag ctatctgtat ccctccatta
     6541 gcattggggc tggcatcatt gattttaaaa accaaattta cgaaaacaga acgagaagct
     6601 ggtaaggcgt ccttagcaat gggactgttt gggattacag aaggcgctat tccgtttgct
     6661 tcaacagatc cactacgggt gattccaagc attgtaatcg gggcaatgac aggaagtgtg
     6721 atcgcgatgc tttcaggagt gacagatcat gtagctcatg gcggtccgat cgtagcggtt
     6781 ttaggagcag tggatcacgt agccatgttt tttgtagcag tgattgctgg tattgctgta
     6841 accgtgctaa tgttaagaat ccttaaaccg acattacaac aaactgcggt tgctgcagca
     6901 aatggtgtga cagtagcaga tctatcagag actgaaacaa gtgatgttta tacgaaaact
     6961 gaaacttttg attctatcac ggatattatg tcagatgaag caatcgtttt aggtgaaaca
     7021 gcaacaacaa aagacggagt aatcgaagat ttagtccaac gtttagaaaa aacagcggcg
     7081 atcacagata aaaccagatt taaagaagtc atctatgata gagaacatga aagtacaacg
     7141 ggaatcggta tggggattgc tattccacat ggaaaatcag cttatgttct taaaccaaca
     7201 gtggcatttg ccagaagtca atctggggta gattggcatt ctttagatgg aaaacctgca
     7261 catatgatct ttatgattgc tgtacctgaa aacagccaag gtgatatgca cttgaaaata
     7321 ctgcaacgtc tatcacgtaa attgatggat gatgacttcc gtcaagcact gatggaagcg
     7381 ccagataaaa caacagttca ccaattatta agcgaaatgt aacataagat gaaaaatcga
     7441 ccgtgatgag acataataga tgtgttatca tgatcgattt tttatttaga agggagtttc
     7501 tgcacattat gaatcagcgt cagcaatctt tgatctatca attaagtgag aacaataatt
     7561 atataacaac aaaaaagcta gcagagcatt tccatgtgtc agaaagaacg attcatagcg
     7621 atttgaatac cattgaaact tggttgagag acaaacatct gcctttaatg tttgaaagaa
     7681 aaaaaggtac aggtatcttg ttgaatggtc tgcctcacga aagggaacaa ctgaaacgag
     7741 cgcttaatga agagactcaa acagaaatgg ataaaaaaca gcttcgtcag ttattgattt
     7801 tccatctgct agtagcaaaa gacggattta gtcttgaaga actttcagaa aaactatatg
     7861 ttggaaaacg tacgattcgt aaagaattga cagagttgca aagctttttt gagtatcacc
     7921 atttacagct agtatccaaa acaaaactag gaacttttct taaaggcgat gaacaagaaa
     7981 aaagacagtt gcttgttaag acattacgaa atatgcagaa agaagatcca caaagtccgt
     8041 cgttgaaaga attcttcgct aaagatacat taaaagttat ccaacataca ttacaagatg
     8101 ttttttatga aaaccattta gaacagccga atggactggc tagtgtggag attcacatct
     8161 actttatgct cgagcgaatg aaacagtacc agaaagtgaa attaagtaaa gacgaaaacg
     8221 aagtcgtgga acacacccaa gcacaaaagt taagtagtca aatattagca aaactagcca
     8281 ccatctatcc aattgaattt tctcctgatg aaatcaatta tctggctttg cgtatcgcca
     8341 atctttttac aaattcacaa gcagccacac gttttcaaaa ggaatctagc gcacttgctg
     8401 atcatttgat cgaacaagtg gaacagtttc taggctattc attaaaagaa gatcagctac
     8461 taaaacaaaa tttaaggtct catttatctt caacttattt tcgactccat tatggtttgc
     8521 agatttctaa tccattgacg aaaaatgtgt ttagtacgta cacacaactt ttcttagttt
     8581 tacagttaat tttagaagat tattttgaaa aggaaaattt ttatgttcca caagaggaga
     8641 cagcttattt aacgatccat ttccaagcag cgatcgagcg acacaaacat cagaaaagta
     8701 gaagatatca aacggtttta gttagtgaat attcaaaagc tatggctact tttctcgaag
     8761 cacggctgaa cagagaactt ccagaattaa cgattatgga tctagttgaa tataagccag
     8821 aattaaagag acaagatttt ccctctgttg attttatctt ggctacgtta ccatttaaac
     8881 atataagcat tccagtaatc gagatctcac caatgatcac agaaactgat ctagcctatt
     8941 tgacaaaata tatgctggag catgtgccta tcaagaagaa aaaaacattt gatctagcaa
     9001 gctttactca tccatttctg atctttcctc aacttgaacg gacagaccca gccgatattt
     9061 taaactttat gggaaacgtc ttagtggaac atcattatgt ggagccggaa ttcatcgatt
     9121 ctgtcttgga aagagacagt cactccagta ctagagtagc accatttgta acaatccctc
     9181 acggtaatcc gctgtatgtg aagcattcga tgatttctat tgctacaatg aaagaaccag
     9241 tcctatggca cggggaacag atacgtatcg tactgctatt agcaataaag aaaaatgatt
     9301 tgaaacatgc agagttcaaa aaaatatttt ctgttattca ctatctagaa aagcagccag
     9361 aacaaatggc tcagttgatg caatccaatc atccgctaga aatcttaacg ttgttatcta
     9421 gttatgaata agtagcgatt aaaaaactgc ctttatattt ataataattc tatctattta
     9481 tttggaagta gaaaactaaa tacaaaaaaa gaggatgtgg ccaaagttgt ggcacatcct
     9541 tttattttga gtttcttata attttttata gttgcttacg atgtcttgac tactggtaaa
     9601 cggtgttcaa gagacaattt gtcggcaata aggcacaatt ttgtaaaata tggaaagcta
     9661 tttttttaac attgtttctt agtgcttgaa aaatcatttt gaatacttct ttatacgatc
     9721 gatgtacgtt gatttaagtc taaaactttt cttttgattc cttctctgac ttttctgaat
     9781 tccgttaata tttcttcttc gctcccgaca gcttgtgcag gatcttttag gtcccagtga
     9841 aggtgttcgt gattgtttgg aacggcaggg cacttgtctt tggcatcacc acaaagtgta
     9901 atgatcaagt ctgcctgagt aaagtaatct ctatcaatca
//
LOCUS       DAZBTF010000091         9846 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000091 DAZBTF010000000
VERSION     DAZBTF010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.092"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(259..996)
                     /locus_tag="ACHGNS_002512"
     CDS             complement(259..996)
                     /locus_tag="ACHGNS_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFN6938407.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(998..2500)
                     /locus_tag="ACHGNS_002513"
     CDS             complement(998..2500)
                     /locus_tag="ACHGNS_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFN6938408.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            2759..4753
                     /gene="uvrB"
                     /locus_tag="ACHGNS_002514"
     CDS             2759..4753
                     /gene="uvrB"
                     /locus_tag="ACHGNS_002514"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFN6938409.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            4763..7579
                     /gene="uvrA"
                     /locus_tag="ACHGNS_002515"
     CDS             4763..7579
                     /gene="uvrA"
                     /locus_tag="ACHGNS_002515"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFN6938410.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            7842..9107
                     /locus_tag="ACHGNS_002516"
     CDS             7842..9107
                     /locus_tag="ACHGNS_002516"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFN6938411.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            9111..9842
                     /locus_tag="ACHGNS_002517"
     CDS             9111..9842
                     /locus_tag="ACHGNS_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFN6938412.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
BASE COUNT         3160 a         1729 c         2117 g         2840 t
ORIGIN      
        1 ttttttgtag ttgtcatgtt tgtaccaaca aaaaagccag ctttagaata agataatcat
       61 catagtttct gaaggtggta tcgactatgt acttgatatc gtcaatgaac cgatggtact
      121 ttcttatgcg atggaacagg tagagcgatt gcttgtttgt gcaggagaac aagcgatgcg
      181 gctggtacag ttaagtgaaa aaacaagaaa ctaaaaatgc ccggtcgtca tgaagcgacc
      241 gggcattttt ggtttttatt aaatatttaa tactttattc aagaaatctt ttgtccgttc
      301 attttgcgga ttttcaaaga tttgttgagg cgttccgtct tctaaaaagt ttccgtcgtc
      361 aatgaacatg acacgattgg ccacttcttt agcgaagccc atttcgtgcg taacgataac
      421 catcgtcata ccttgttctg ctaacttttt catgacgttc aagacatcac cgaccatttc
      481 ggggtcgagt gcagatgtag gctcatcaaa aagcatgatg tcagggttca tcgctagagc
      541 acgagcaata gccacacgct gcttttgtcc accggaaagc atttcaggat acatatcttt
      601 tttatcttct agaccaactg tctgcagcaa tttgattgct tttttctctg cttcttctct
      661 agattctttc ttcaggtcca tcggtgctaa aatgatgttt tccaaaatag aaagatgagg
      721 gaataggtta aagtgctgga ataccattcc gatatgttgt ctcaccaagt tgatatctgt
      781 gcttttatcc atcaagtggg cgccgtcaat aataatttca ccgtcagaag gttcttctaa
      841 acgattcaag caacgcaaaa atgtactttt tcctgaacct gaagggccaa tgatacagac
      901 gacatctcct tcgttgatgg acacattgat gtcgtttaat actgtgttat cgccgtattt
      961 tttgactaaa tgctcaacta agattttttc tgccatctta cttcaccttc ttttctaaaa
     1021 cttttgctaa ttttgtcaat gctgtgatca aaatcaagta catgatcgca atgatcaagt
     1081 aaacatacgt actttgtgtt gtacgtgcaa caatgatctt accagtttgc agcaattcga
     1141 ctacaccgat tgcggaaatg atcgttgtgt cttttaatga gataacgaac tgattgacga
     1201 atgatgggat catgatgcgt atcgcttgcg gcaaaatgat tttttgcatt gtccgattat
     1261 aagaaagacc taaacttctg gaagcttcca tttgtccgac aggaacggcg ttgatcccgc
     1321 ctcgaacaat ctctgcgata taagcacttg cgttaagagt caaggtgata acaccagcaa
     1381 ggaagtcagg aatcgtaaat ccaacgatac caggcaaccc aaagaaaata aagaacgcaa
     1441 gaaccatcat tggaattcct cggatgatat cgacataaat actagcaatt gtgcgaagag
     1501 cgcgtattgg tgctacactg aataatccaa aaatcacacc gattactata gccaaagcaa
     1561 aggagatcaa agttaggaga attgttctcc aaagtccttt caacaatgct tgatagttgt
     1621 tttggataag tccaagaaat gttgattcat cagcagtgga agactcactg gaagtttctg
     1681 aaccatccgc tacatattct ttaataatct tatcgtattc gcctgttctt tttaattctt
     1741 tcaatccttc attgaacata ttaagaagct caggattttg tcctttttta acggcaaatg
     1801 catattttcc gccagattct tttttgatcg gtgtttccaa ttcttgtcct tgtttgatcg
     1861 cataaccgat taccggataa tcatccatca ttgcatcgat tgcgccaact tttaacccgt
     1921 cgtacaattg atcagcagca tcaaaatatt tgatagaata gccatattca gattcatggt
     1981 cgcttaaaaa ttcagcactt tcggtaccaa cttttgctcc gactgttttt cctttcaaat
     2041 cttcataaga cttgatcgaa gtatctcctt ttttgacagc taattggatc ccgctatcaa
     2101 agtaagaatc agaaaaatca tagcttttct ttcgttcatc tgttacagtc attccggcaa
     2161 tcattccgtc tgcttgtcct gattccaaag cttgcaaagc agcagagaaa ccacggtagt
     2221 ccattcggat gttaaatcct tgcagtttgg cgatgttttc catcaatttt acatcgatgc
     2281 cttcgtactc gccctttgcg ttttgaaatt caaatggagc aaaagcagag tcactggcaa
     2341 ttacgtatac gtcttttttg ggctctattt tttgcattgt ctcgcttgtg cttgactctg
     2401 ccgttgtagt gttgtcagct tgtgcaacat gtccaaacgc aagtgtcaca agtcctgtca
     2461 ttaagaaaac aaatgagtga aataaatgct ttcttttcat aatcaacctc ctaaatttcc
     2521 aatattttat tattataggc gaaaagtgct gaatcagaca agactgtaag ctcgaaaaaa
     2581 tgaaaaaaat gcgagtttct aacattttat gttataaaac gtaacatgca taataagata
     2641 aaaaagtggc gaaagtatgt tcggtgtggt ataatttttt agtaaggaat gcctttgctt
     2701 tccttttctt tttattggtc gtaaaatgaa aaggaatgaa tgaaaaggag gacgaagaat
     2761 gattgagaga gatacctccc gacactttga gcttgtctcg aaataccaac cggctggtga
     2821 tcagcctgaa gcgattaacc aattagtgga tggcgtagtc ggcgggaaaa aagcacaaat
     2881 cttattaggt gcgactggaa cagggaaaac atatacgatt tccaatctta ttgagaaagt
     2941 gaataaacca acactaatta ttgcccataa caaaacattg gctggtcagc tgtacgggga
     3001 attcaaggaa tttttcccta acaatgctgt tgaatatttt gtttcttatt atgattatta
     3061 tcagcctgaa gcttatgtgc cttcaagcga tacgtatatc gaaaaagatt caagtgtaaa
     3121 tgatgagatc gacaaattac gacattcggc tactagttca ttattagaaa gaaatgatgt
     3181 gatcgtcatt gcatccgttt catgtatttt tggattaggt tcgccttttg agtaccaaaa
     3241 acaagtagtt tcgattcgac agggagcaga actagatcgc aatcagttga ttcgtgactt
     3301 agtcagtatc caattcgaaa gaaatgatat agattttcag cgcggtcgtt ttcgagtgcg
     3361 cggtgatgtc gtggagattt ttccggcttc aagagatgaa cgtgccttac gtgtggagtt
     3421 ttttggagat gaggtcgaac gtatccgaga agtcaatgca ctgacaggtg aagttttagg
     3481 agaaacggaa cacgtggcaa tcttcccggc gactcacttt gtgacgaatg atgaacatat
     3541 ggagcatgcc gtagcgaata tcaaagcgga attagaacag cgtttaactg tcttgcgaaa
     3601 tgaaaacaaa cttctggaag ctcaacgttt agaacagcga accaattacg atattgaaat
     3661 gatgttagaa atgggctaca cgtcgggaat tgaaaattat tctcggcata tggacggacg
     3721 taaagaaggt gaaccgcctt atacgctatt ggatttcttt cctgaagatt ttttgatcgt
     3781 ggcggacgaa tcgcacgtaa ccatgcctca agtcagagga atgtataatg gtgaccgagc
     3841 gagaaaacag atgctagtag attacggttt ccgtttgcca tctgctttag acaatcggcc
     3901 acttcggtta gaagagtttg aaaaacatgt caatcagatc atttatgtct ctgcgacgcc
     3961 aggaccatat gaacatgaac aaacagatac cgttatccaa caaatcatcc gtccgacagg
     4021 gctgcttgac ccagtgatcg aagttcgtcc aatcatggga caaatcgatg atctagtagg
     4081 cgaaataaat gaacgagtcg aaaaagacca gcgggtgttt gtaacgacat tgacgaaaaa
     4141 aatggcagaa gacctcacag attatttcaa agaattaggg atcaaagtca aatatttgca
     4201 tagtgatatc aaaacgctgg aacgaacaga aatcattcga gatcttcgat taggtgaatt
     4261 tgatgtgtta gttggaatca atttgcttcg tgaaggatta gacgtacctg aagtttccct
     4321 tgttgctatc ttggatgcgg ataaagaagg ctttttacgt agtgaacgtt ccttagtcca
     4381 aacgattgga cgagctgctc ggaatgaaga aggtaaagta atcatgtacg cagacaaagt
     4441 aacagattct atgcgtctag cgatggatga aacatcaaga cgccgtacga ttcagcagaa
     4501 gtacaatgaa gaacatggga tcgttccaaa aacgatcatt aaagaaatcc gagatttgat
     4561 ttctattacg aaagaatcgg aagatgatac aaaagaagcg gtacaagtct cgtatgaaga
     4621 aatgacgaaa gaagaaaaag atacgttatt gatgaaatta gaaaaagaaa tgaaaaatgc
     4681 ggcgaaagca ttggattttg aaacagccgc taatgtgcga gatatgattt tagaattaaa
     4741 agcttcaaag taggaggaca acatggctaa cgataaaatt acgattcacg gtgcacgtgc
     4801 ccataacttg aaaaatatag atgtaacgat tccacgagat aaattcgtgg tcgttacagg
     4861 tttgtccggt tctggaaaaa gttccctagc atttgatacg ctgtacgctg aagggcagcg
     4921 gagatacgta gagagccttt ctgcttatgc tcgtcagttt ttaggacaaa tggataagcc
     4981 ggacgtagat agcatcgacg gattgagtcc ggcgatttct attgatcaga aaacaacaag
     5041 taaaaatcct cggtcgacag taggtactgt gacggaaatc aatgactatt tacgcttgtt
     5101 gtttgcacgt gtaggacatc cgatttgtcc caacgatcat atcgagatta ccagtcaatc
     5161 agtagatcaa atggcagatc aagtaatggc tttgcctgag cgcacaaaga tccaagtgtt
     5221 ggcaccgatc gtggtaaaga aaaaaggaca acataaaaaa atattcgaac gaatccaaaa
     5281 agagggatac gtccgcgttc gagtcgatgg tgagatctat gatttaagtg aagtgcctga
     5341 gctggagaaa aacaaaaaac atgatatcgc gatcgtcatc gaccggatca ttgtgaaaga
     5401 agggattcgt tctcgtctat ttgattcttt agaggcggct ctgcgattgg cagacggcta
     5461 tgctctggtt gacgtgatcg gtgaagaaga aatgttgttt agcgaacact atgcctgtcc
     5521 ttattgcgga tttactgttg gcgaactaga accaagactg ttctcattca atgcgccttt
     5581 tggagcttgt ccagactgtg atggtttagg tgtcaaatta gaagtcgatc aagatttagt
     5641 gattccagat agaacgaaga cacttagtca aggtgctatc gttccatgga atccaatcag
     5701 ctctcagtat tatcctcaaa tgttagcgca ggcttgtgaa agtttcggta tcgatttaga
     5761 tacaccattc gaagaattac cggaagagca tcaggatatc atcttgaatg ggacagatga
     5821 tcttttccat ttccattatg aaaatgattt tggtggcgtt cgcgatgtag aaacgacttt
     5881 cgaaggggta ttgaagaata tcgagcgtcg ttatcatgat acgaacagtg atttcacgcg
     5941 tgagcaaatg cgtctttata tgacagaatt aacttgtcag acatgtaaag gttatcgatt
     6001 gaatccccaa gctctatctg ttcaaatcaa tggaacgaat atcgggcaaa caaatgagtt
     6061 gtctattcaa aaagcagtag atttcttctc aaacttaacg ttatctgaac aagagaaagt
     6121 aattgccaaa ccgattttaa aagaagtaaa tgacagactt tcattcttgg aaaatgtggg
     6181 actagactat ctaactttga gtcgcgcttc cggaacatta tctgggggag aagcgcagcg
     6241 aatcagattg gctacacaaa ttggatcgaa tctttctgga gttttgtata ttttagatga
     6301 accatcgatc ggattgcatc aacgcgacaa cgatcgactg attggctcat taaagaaaat
     6361 gcgtgatctt ggcaacaccc tcgttgtagt cgaacatgat gaagatacga tgtgggcagc
     6421 agattatctg attgacgtcg gacctggggc cggagaacaa ggcggacaaa tcgtggctgc
     6481 aggcactcct gaagaagtag ctaatgatga aaattctttg acagggaaat atctttcagg
     6541 gaaaaaatca atccctgtac caaaagaacg ccgtaaaggt aatgggaaag caatcaagat
     6601 cactggtgct tcagaaaaca atctgaaaaa tattagtgtt gaatttccat taggagaatt
     6661 tgtagcagtc acaggtgtgt ctggttctgg aaagagtaca ttggtcaata gtatcttaaa
     6721 gaaatcgtta gcgcaaaaat taaataagaa ttctgctaag ccaggtaaat tcaagacaat
     6781 ttccggctac gaaagtatcg aaaagatcat cgatatcgat caaagcccaa tcggccggac
     6841 gccgagaagt aatccagcga cttatacaag tgtatttgat gatatccgtg ggttatttgc
     6901 tcaaacgaac gaggcaaaaa tgcggggtta taagaaaggg cgttttagtt tcaacgtaaa
     6961 aggcggtcgt tgtgaagctt gtcgcgggga tggaattatt aagatcgaaa tgcacttttt
     7021 gcctgatgtc tatgttcctt gtgaagtatg tcatggcaaa cgatataact ctgaaacatt
     7081 agaagtgcat tacaaaggaa aaagcattgc tgatattttg gaaatgacag tagaagatgc
     7141 tgtagaattc ttcaagcaca ttccaaagat tcatcgcaaa ctgcaaacga ttgttgatgt
     7201 tggcttaggt tatgtgacga tggggcaacc agcaacgaca ttgtccggtg gtgaggcaca
     7261 acggatgaaa cttgccagtg aattgcacaa aatctctaat ggaaagaatt tctatatact
     7321 agatgaacca acgacaggac ttcatagcga tgacatcgcc cgcttgttgc atgtattaca
     7381 aagattagta gatgctggta acacagtttt agtgattgaa cacaatctag atgtaatcaa
     7441 aacagcagat tatatcattg atttaggacc agaaggtgga gaaggtggag gaacgatcct
     7501 tgcgactgga acaccagaag aaatcattaa cgtaaaagaa agttatacag gtcactattt
     7561 gaaaaaaata atggtataaa gaggatagat agctgagata gttaaaaaaa cataaaaaaa
     7621 tgaatttttg cttgttttat taatatcagt gttctagaat cattacatgg tagttctttc
     7681 tacactgatt ggtatagaca aaaacttcct ataagttcaa tccgttcaaa aaagaattga
     7741 gcaatcggag gagtttcctt tgattgcttt tttctttatg gtatgatatt ccttgtgtag
     7801 ttggaaaaga aaaacaagag attatgagta agtgagagat aatgatgaac agtattgaaa
     7861 atgaagaatt tatccctata ttattaggaa gcgacatgaa cgtttatggt atggcacgtt
     7921 cttttaatga agcatacggc aaaatctgcc aagcatatgc ttcggatcag ttagccccta
     7981 cccgctacag taaaattgtc aatgtagaag ttattccagg atttgataaa gaccctgtgt
     8041 ttatcgagac gatgcttcgt ttggcaaaag aaagatattc tgataagagt aaaaaatatc
     8101 ttttgattgc ttgtggtgat ggatatgcag aattgatctc ccaacataaa caagagctgt
     8161 cagaatattt tatctgtcca tatattgatt attctttatt tgaaagactg atcaataaag
     8221 taagctttta tgaagtttgt gaagaatatg atcttccgta tcctaaaaca ttgatcgtca
     8281 gagaagaaat gttagtaaat ggacatttgg aacaggaatt gccttttgaa ttcccagttg
     8341 ctttgaaacc tgcaaacagt gtggagtatc tttctgtcca atttgaagga agaaaaaaag
     8401 cttttatttt agagactaga gaagagttcg atttaatttt aggccggata tacgaagccg
     8461 gttataaaag tgaaatgatt gtacaagatt ttatcccggg tgatgatagt aatatgcgtg
     8521 tgctaaatgc gtatgtagat gaagatcatc aagttcggat gatgtgtctt ggtcatccac
     8581 tgttagaaga tcctacacca gcatcgattg gtaattacgt tgtgatcatg ccagattata
     8641 acgaaaaaat ctatcaaacc attaaggcat ttttggaaaa aatcgaatat acaggctttg
     8701 ccaatttcga tatgaagtat gatcctagag atggagaata taaattattt gaaatcaatt
     8761 tgagacaagg tcgtagcagt ttctttgtga cattgaacgg cttgaatctg gctcgtttcg
     8821 tcacagaaga tcgagtgttt aacaaaccct ttgttgaaac gacctatgga acgaatcaat
     8881 cagataaagc aagattatgg atgggtgtgc ccaaaaaaat ctttttagaa tatgctcggg
     8941 aaaatgaaga caaaaaacta gctgagcaaa tgatcaaaga aaatcgttac ggtactactg
     9001 tgttttatga aaaagaccgg tcaatcaagc gctggttgtt gatgaaatat atgttccata
     9061 actatattcc aagattcaaa aagtattttc atgtgaaaga aggctagttc atggagaatt
     9121 ttttcagtat tttaggcgga atgggcacga tggcgacaga aagttttgtt cgcttgatca
     9181 accaccgaac gaaagctaca aaagatcaag aatatttgaa ttatgtctta ttcaatcatg
     9241 caacagttcc tgatcgtaca gcttatattt tagatcgatc agaagaaaat ccaatgccgt
     9301 ttctgctgga tgatattgag aaacaaaatt tattgcagcc gaacttcatt gtgttgactt
     9361 gtaacaccgc acactacttt tttgaagaat tacaagcggc aacggatatt cctatccttc
     9421 atatgccaag agaagcagca aatgaacttg tccgtcagca tacgacaggg agagtagcga
     9481 ttctaggaac agaaggaagt atgaaagctg gaatctatga acgcgaagtc aaaaatcttg
     9541 gattcgaaac agtgattcct gatactgttc ttcaagaaaa aatcaattat ctgatctatc
     9601 atgagatcaa ggaatcggat tatttgaatc aagagctata ttacgaaatc ttagaagaag
     9661 cggtagagcg cctaaactgt gaaaaagtta tcttaggttg cacagaattg tcattgatgc
     9721 atgaatttgc tgaagataac cattatccag tgatcgatgc ccaatctatt ttagcagatc
     9781 ggacgattga acgagcgcta gctgaaagaa gcaaggcatt ggatacagca tcggaaaagt
     9841 aaaaaa
//
LOCUS       DAZBTF010000092         9716 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000092 DAZBTF010000000
VERSION     DAZBTF010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.093"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            69..3347
                     /locus_tag="ACHGNS_002518"
     CDS             69..3347
                     /locus_tag="ACHGNS_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFN6938413.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3888..5795)
                     /locus_tag="ACHGNS_002519"
     CDS             complement(3888..5795)
                     /locus_tag="ACHGNS_002519"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFN6938414.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5925..6800
                     /locus_tag="ACHGNS_002520"
     CDS             5925..6800
                     /locus_tag="ACHGNS_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFN6938415.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6880..7854
                     /locus_tag="ACHGNS_002521"
     CDS             6880..7854
                     /locus_tag="ACHGNS_002521"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFN6938416.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8034..9179
                     /locus_tag="ACHGNS_002522"
     CDS             8034..9179
                     /locus_tag="ACHGNS_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFN6938417.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9316..9660
                     /locus_tag="ACHGNS_002523"
     CDS             9316..9660
                     /locus_tag="ACHGNS_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFN6938418.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
BASE COUNT         3170 a         1797 c         2055 g         2694 t
ORIGIN      
        1 tgacagtaaa aataaattag gtaatacttt aagtaaacgc tttctaattt gttacgggag
       61 gaatacactt gttaaaaaaa cgtataaaat acagtgcgct aatgatgaat ctccttatgc
      121 tcagtacccc ggttttaatt tctgtcgttg aagttgctgc taacgaacaa cagtctgtga
      181 acgaagaaaa tgcacacgtg aacattgaag ggatgtcaac aatggaggaa ataccaagaa
      241 ctagtgaaga tatggatacg gaagaaggaa atgatcctat gatgaaagaa tcagaaaatg
      301 aaaagacaaa aaagcaatct gaagaagcaa atgaagagtg gaaaggggtt gttttcggag
      361 aaagcacaag tgctagtagg tatgccattg aaccattaga agacggcgtg cggctctctt
      421 ccacgaataa cggcggaaaa ttccagtcaa gcggatcaga tggaatgacc tattattatc
      481 aagcaatccc caaagacatc aactttagga tgcgggcgac gatcgaaata gaatcttgga
      541 cctatacgaa tgcccaagaa ggatttgctt taatggtaag agacgcagta ccaaaaaatc
      601 atttgtttgg gcaggcattt tattcaaatt catttgccat actaggcagt cggatcgaat
      661 atgtctggga ctcggataga caagaagtgg tcaacacgag cggcagcaaa tatacgatgc
      721 gacttggatt ggggacacgg gcaatcacag gaatatcatc tgaagatccg caagctgcgc
      781 cagctcctgg gagtgtctca gtggaaacaa cacctttaga gacttcagca gggttcttgg
      841 aaaaagaaac aggttcttat aatatattcg gaaacggtca tgggaatctg tttccggcca
      901 cgaactcgat tactaaactg gacgtagaga tgaaaaaaac gaatactggt ttagaggcat
      961 attattacga tcctgaaaca ggagaagaaa tggcttctga catcatgtat gactgggaaa
     1021 aattatacgc ttcagatgaa tcggttattt atgcaggatt tgctgcagca aggaatatga
     1081 cgatcaaggt agaaggaatc gaagttgctt actcagatcc tgcgacagat cctcctgggc
     1141 aagaaagacc tactcgcttg attgatccga tttacactgt gacctctagt aatcattcag
     1201 gaaaccaaga tcatactatg tcctttagag caaatgctga cggattattg acaattaaaa
     1261 ataaagcaac aggagaggtg cttaagaacg caaaaaactt ggctatcact gcgaatcggg
     1321 aatttgatta tcagacaaaa ctgatggaag gaacgaacga atttactttc agttttacgc
     1381 cagataaaaa ctatccgcca gaagaatacg tagagatgac tagttatgaa acaaaagaga
     1441 ttcttcatac agtggactac cgtaaaccta aatattcgac agtttatgta acaccagaag
     1501 ggtcagatgc aggaaacgga agcagacaaa atccattatc tctgacacaa gcattgagat
     1561 acgcatatgc tgggcagaca atcgtcatgg cgcctggaac ttattccttc gaaagaggat
     1621 tgacgattcc aaaaggcgtt aacgggacaa gagaaaaccc gattcagttg attgcagaga
     1681 aaaatccaga aaacaagcgt cctgtacttg attttaaagg aactggaaat gggatgacac
     1741 tgttcagtca ctactggggt ttacgagggt ttgacattac aaatagtgca gctatgcaaa
     1801 aaggattaca gatttctgga aatcataatt tgatcgaaga gattcatgct tatcggaatg
     1861 gaaatacagg aatccaaatc agcggtagct caaatgatcc tttcgaagca tggcccgcgc
     1921 ataacttagt caagaactgt acatcttttg aaaatgccga tccaggattt gaagatgccg
     1981 atggatttgc ggccaaactg acaataggag aaggaaatgt atttgacggt tgtatcgcgt
     2041 atcataatgc agatgacggt tgggaccttt ttgctaaaaa tgaaacaggt tcgatcggaa
     2101 aagtaatcat caaaaactca gttgcttatc gaaacggctg ggtaccagga attgagggag
     2161 aaggtaacgg taatggattc aaaatgggcg gttcttcttt aacgggccct catgaattga
     2221 tcaatagttt gtctttcgaa aatcttgcga aaggaatcga ttccaatagc ggaacagata
     2281 ttatcgtcaa tagcagtaca agttttaata acggttcata taatgtggct ctttatacct
     2341 cttcagcagg aaacacagac taccatgctt ctggtatctt atctttccga acagagaatc
     2401 ttgagatgag agagcagatc cgcccattga atcaaaacga agatcaaatc taccactcgt
     2461 tgaactacta ttggaatgaa cgagagaaac aatcagaaaa tactcttgga cataccatat
     2521 caaaagattg gatcgagtct ctaaatacat gttcgaaaga aggtcagcag cctgttacgc
     2581 gctatgaaaa tggaagtatc catgttcatg gactgatgca gattaaaccg gggaatcgtc
     2641 aaacagaaaa ggaaagagtg gatggtcttc cattatctgt aggtgctatt tttgacggag
     2701 aaacctcacc gtcttcggaa tatccagagt atgcaatcgt acaagaacca aatcagacaa
     2761 aaccaaatga acaaaataat ccgattatta tacaagaagc aaatggtgtg gcggagaatg
     2821 cagaaggaaa tatcattgaa ctgaagcctt ttacagtttt tgtgtcaact ttcacaccaa
     2881 atcaaggaac gcctgtcttt gaagatatgc gttatttccc agaaaagatc caaatcgttg
     2941 aaacacagtc aagtgctcaa ttttctaatc ataccgctat tttttcagca ccttcaaaag
     3001 gagaatatac gctagaagtc acttatcgtt cggaaatatt tgatggggaa aaatggtctg
     3061 ctcatacaga atctgttacc cagcaaaaag cgatcactgt gcgagaaact gccgatgtgc
     3121 ccccgggaaa tgacaaacca gaaagagaaa atccaggaaa tgaatcacca aatggaaaac
     3181 caaatggaaa accttctcat ccggatagga cagcaaaaga aagtgaaaaa aatggcactc
     3241 aaggactccc aaaaacggga gaatttaata acccgttgct agcattagct ggaggaattt
     3301 tgttaatcgg tgtagttgtt tatgtcatga aacagcgaaa aaaataagtg gaatttctcc
     3361 ttttatgatg taaaacaatt gactgaggaa agtaaaaata gaaggaaatg agaccaatag
     3421 atgtctcact tccttctatc tttttataga tgaggttctc cttgctgctt ttgattggct
     3481 gttcttatta cttgatgcag gacagctttt tcacaatctc tccactaaac ggtgttcaat
     3541 cagctgaccc tcggtattag ctcaacaaac ggcaaaatat gaggaactat ttttccgttt
     3601 ttttctctaa tactcaggtc aggacgctga tttcacaacc tctcgataaa cggtgttcaa
     3661 aagccagttt ctcggtagta agtcaaattt atgcaaaaat gtggaaagcc attttcacaa
     3721 aatttttctt actacttgaa actaagcagc tttttcacaa cctctaatac atggtattcc
     3781 agaagtaact tcttcgaaaa ataagccgaa atgtcacaaa aatttgagaa gcgattttcc
     3841 caaattcctt cttatttctt gaagctgacc acttctggca caacctctta ccagattcgc
     3901 actctttttt ctggcgctag atacatagcg tcttgttccg tgataccaaa tgtttcatag
     3961 aattcgtcaa ggttttttgg ttgcacgttt gctcggagtt tagcaggggc atggacatcg
     4021 atttgcagta aaagttgttg gtattctttt tttgcttttg tacgccaaat cgttgcccaa
     4081 ttgacaaaga attcctcgat agaaagatct gattccgttt ttgctgcttc caatgcacag
     4141 cttagaccgc ctgcatcagc aatattttcc gaaacggtta attttccgtt tacttttccg
     4201 tcagcaaaag gcagaccatc gaattcttcg atcattgctt gtgctttgtt ttgaaattgt
     4261 gtaagatctt cttctgtcca ccagttgttc aagttgccat attcgtcaaa taaagcgccg
     4321 ttattatcaa atgcatggga aatctcatga gcgataacag ctccgattcc accataatta
     4381 gcagagcttg attgttctag actgtaaaat ggtgcttgaa ggatcgctgc cggaaagacg
     4441 atcacattcc gaaatggatg ataataagca ttgaccgtgt cagcactcat ttcccattct
     4501 gaacgatcca ctggtttgtt ccatttgctg aaacgatctt ttaatgtgag tttgctgaaa
     4561 acaagagtat ttgaaagcaa agtaccaccc tgatctgcag gtactgtttt gaactggtcg
     4621 aataatgctg ggatcttatc ggggtagcct acttggatac ctaagttatt taattttatg
     4681 acggcttttt cttttgttgc atccccaagc cagtcatttt cttctagacg ttttttataa
     4741 acggcgatca tttttttcac catttgttct acgtcttttt tagcagcttc gccaaaatat
     4801 ttttttccgt agtaatcgcc aactacttga tcgaattgtc cagaggcaag gtaatacgct
     4861 gcttttttct gaggcattgc ttcgtctgtt ccagacaata cgcgagaata gattccgctg
     4921 acttggcgga attcttctga tagataacca ctaagagaac gtactgtttt aatgatcatc
     4981 cagcttttga ataacggaaa atgagcttct gtcaggatgt cattcaaatg atcgaagtac
     5041 actggatcag ttacgatcac tttatctgga gttgtcccaa tcaaacccgt gacaagtttt
     5101 ttcaagtcca gtttatctgt gtatttaata aatgtatcaa aagattgcgg attatacatt
     5161 ttgctgtaat ctgcgttttc ttctgcactt tttacatgag gagcaatcaa tttatcaaat
     5221 tgcactgctt gttccgtgat tgcttctgcc tgactttttt ctttgccggc aagctctaat
     5281 aactgaacag ccatatcaaa gaaaacgcct attagttgtg caccgttggg atggtctgat
     5341 gcataatacg tcttatctgg aaggaataaa gaaggcggat aagcaaagaa ggcgttcgtt
     5401 tgcgcattct tcatatctgc atctacatcc agattaaatg gcaatggtaa tgtatccaac
     5461 agccattctg gaaattgctt attcaattcg tcgaaagact gcaatgattc aactttttcg
     5521 attataggtt gaagcggttg agcatgcaac tcatttcttc ggtcataatc gtttgccagt
     5581 tgataatatg caaggaaatg tttcattttt tctgaagaca ctttttctgg atgggcaagc
     5641 agatcgtctg tttctcccat caaaagttgg tcgatcccgt caactaaatc ttggaaaccg
     5701 cctgtagctg gtttatctgc agggattttt gctgtttttt gccattcttc attgactgct
     5761 tcatagaaat cttgtttcaa tcgttctttg ttcattcatc gatcgctcct tttttgtttc
     5821 gtgtgcccat tatcttacaa aatgaaggaa ttaacaaata gaacataaaa aattgaaaat
     5881 tcgaacaatc aaactgattt gtttttttca atccttcact tataatgaaa aaaaggaggc
     5941 gatcccaact gaatcaagta gtggataaac ctttatattt caaagtagat aaaaaaatta
     6001 aaaaattagc cagcactcag cagctgcagt caagaaaaag taaattgctt tttttagctc
     6061 tggtttttga agatcaaagt tatgtgatta tcgatcaatc aggacatccg gtagaatatt
     6121 ctccagcaaa atatacttat caagagggtc tttcttgtaa aaaatggaag ctgatcaatg
     6181 aagagccaat cgagttatct agatggatca acagaaaaga agatattccc gttttgatcg
     6241 aagaaaaaag gtcaggaaag gaactcgcaa attgctgggt gggattgcca gaagaacgtt
     6301 ttttgaggta taagaaatgg gctaccccct caggctatct ttgtggtact tacgcggcag
     6361 cagttctcct tgcctattat caagactatc gtaaggggtg gatgcttcca aatgagattc
     6421 gcaagaaaaa tacggctgat tcgttggttt tgacgaaggc attgcgtagc cagatccaac
     6481 cactaggact tcctacgatt ccttttcagg ttagtacagg gatttctagt tttttaaaga
     6541 agaatggcaa tcatgagcgt gcacgagcaa ctcttctagg ttcatggcag cgagctacaa
     6601 aacgtatacg tgaaggcaaa cctgtgatga ttggtatttt gaaagtactt ggcagtacat
     6661 atggcaatca ttgggtgaca gcgtacgctt attttgaaac tgaaacagga gaacgttatt
     6721 acaaagtaca cgacaactgg ggcgattatc ataaagtcat tcctgccagt tggagtaacg
     6781 gaacagtttc acttccttaa gaatttgctg aaattaacag aaaatcttgg aaaaatatgt
     6841 taaactatcg aagaatagag aagttttggg gaggactaca tgagtaataa gtatcaggat
     6901 gacacgttga agatttttag tttgaatggc aaccgtccat tagctgaaaa aatcgcgaaa
     6961 gtgttcggaa cagaattagg taaaagcgtc gtgaagcaat ttagtgatgg cgagatttcc
     7021 atcaatatcg aagaaagtat tcgaggggat catgtgtata tcgttcaatc aacgaatgca
     7081 cctgtcaatg attactatat ggaactactg atcatgatcg atgcaatgaa gcgagcaagt
     7141 gcgaaaacga tcaatgtcgt attgccttac tatgggtatg ctaggcaaga ccgcacggcg
     7201 aaacctcatg aaccgatcac tgcgaaattg atcgctaact tgatcgaaga agcaggggca
     7261 actcgagtac tgactttaga tctgcacacg gttcaagtac aaggattctt tgatattcct
     7321 gtagataatt tatttacgat gccgttgttt gctcattatt accgtcaatt aggtctgacg
     7381 ggagatgaca tcgtagttgt ttcgccaaaa aacagcggtg ttcagcgtgc acgtagtctt
     7441 tcagaatatt tgaacagcac attagccatc gtagatcatg cagatgatgc ttcaactgag
     7501 ggtggttatg tgatcggtga tgttcaagga aagacatgta tcatggtaga tgatatcttg
     7561 aacacaggag caacgcttgc acgagcagca aatgtattaa aagaaaacgg cgcaaaagaa
     7621 gtatatgcct gtgcgtcaca tggattattg tccgatcatg cgaaaagtat tttagacaat
     7681 gcaccaatca aagatatttg tatcactgat tctgttttca cagaagaaaa tcgtcatcca
     7741 gaaaacttaa caatcgttac atgttcagaa ttaatgggtg aagcggtcaa acgcatccat
     7801 gaaaatacac cgatgagtcc gttattccgt ctggaagaaa aagaattcga ataaacaaaa
     7861 aaaagaggga aagaagttgt gacagaagtg agcagcttca aggagctgcg acaagacttt
     7921 tgtcacagct ccttttttct atgcccatga aacgtcctct agacaaaaat gagctcttct
     7981 ttggtttgtt cgctatttta tgctagaatg tgcctagcaa taatttggaa gggatgaatg
     8041 aagtgaaaac tatttatctg gatcatgcgg caactacccc catgcatcca caagtaattg
     8101 aagcgatgac ggcaatcatg caagacacgt ttggaaatcc ttcaagcata catggatttg
     8161 gtcgccacgc gcatgaaaaa ttggaaacag caagacaaat cattgcagac agtctgcaag
     8221 caaaacctca cgagatcatc tttaacagtg ggggaacaga aggggataat acagctatct
     8281 tagaaacagc gttctcgcgt caaaaagaag gcagacatct aatcactaca gctatcgaac
     8341 atcctgctgt tcttaatacc atgaaatatt tagagacaaa ggggtttgac gttacatatc
     8401 tgcctgtaga tgaaaaaggg aatctgtcga tcgaagaggt caaaaaagcg ttacgagaag
     8461 atacgatctt ggtctctgtg atgtacggga acaatgaaat cggaaatttg atgccaatca
     8521 aagaaatcgg tgaactttta gcggatcatc cagcttattt ccatacagat gcggtacaag
     8581 cgtacggaaa ccaaacgatc catccagaag aattaggaat cgatttatta agtatctctg
     8641 ctcataaaat caatgggcca aaaggcgtcg gtttcttata taaacgagac ggagtaaata
     8701 tccctgcatt gcttttaggc ggggagcagg aagaaaagcg gcgtgcaggt acagaaaatt
     8761 tagcagggat ctggggtatg gcaaaagcag ttgaactgct gtcacctgaa gaacgtgaag
     8821 cacgtaacaa caaatataaa ggattccagc aattgattct tgatacatta gaagcaaacg
     8881 aaatccctta tcaagtaaat ggtgatttaa cgaataaact ccctcatgtg ttgaatcttc
     8941 gcttaattgg cgaagcaaat gatttgctac tgatgaagct tgatcttaat ggggtcgcta
     9001 tttctactgg ttcagcttgt acagcaggaa atatcgagcc ttcacacgta ttagaagcaa
     9061 tgtatggaga agatacaccg atactaaaag aatccatccg tatcagcttt ggtttgggaa
     9121 ataccgaaga cgacatccag tatttccttg atcaattagt tcgtgctgtc aaaaaataac
     9181 tagtgaagtc attcttatat gaacagatgt ctgagagaac gccttcattt tttagggaac
     9241 ttaactgaaa aacctagtaa aaatcaggat aaacttttat aataaagaga aactaatttt
     9301 atcgaggtga aaatcatggc atttgaaaca acagcaacgg tactgggcgc accaattagc
     9361 tatcgtttgc atcctgatgc aaaaagatat acattgcgcg ataatggatt tacagaaaca
     9421 aacggaggca attatcaatt gatccgacca ttagatgcca cgccgcaaag caaagaagga
     9481 ttcaaattaa aaatcactgt atcaaaagat attcaaacat tgaaaatgtc gatcactaca
     9541 gcaaatggat tgaaagcagt caatattttc aaagatccaa aacagaaaat gagccaagat
     9601 aaattttatt ttctgatgga tgggatgatt agccgtgggt tattcgaaaa agtcgaatga
     9661 tctgtggata taaaaagaca actagttttg aaacggccta ttgttagatg actaac
//
LOCUS       DAZBTF010000093         9311 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000093 DAZBTF010000000
VERSION     DAZBTF010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.094"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(42..710)
                     /locus_tag="ACHGNS_002524"
     CDS             complement(42..710)
                     /locus_tag="ACHGNS_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN6938419.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            844..1131
                     /locus_tag="ACHGNS_002525"
     CDS             844..1131
                     /locus_tag="ACHGNS_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938420.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1547..3085
                     /locus_tag="ACHGNS_002526"
     CDS             1547..3085
                     /locus_tag="ACHGNS_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFN6938421.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3082..4047
                     /locus_tag="ACHGNS_002527"
     CDS             3082..4047
                     /locus_tag="ACHGNS_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6938422.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4034..5356
                     /locus_tag="ACHGNS_002528"
     CDS             4034..5356
                     /locus_tag="ACHGNS_002528"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFN6938423.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5419..5523
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5597..6340
                     /locus_tag="ACHGNS_002529"
     CDS             5597..6340
                     /locus_tag="ACHGNS_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6938424.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6355..7347
                     /locus_tag="ACHGNS_002530"
     CDS             6355..7347
                     /locus_tag="ACHGNS_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN6938425.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7359..8096
                     /locus_tag="ACHGNS_002531"
     CDS             7359..8096
                     /locus_tag="ACHGNS_002531"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6938426.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8086..8808
                     /locus_tag="ACHGNS_002532"
     CDS             8086..8808
                     /locus_tag="ACHGNS_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFN6938427.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8892..>9311)
                     /locus_tag="ACHGNS_002533"
     CDS             complement(8892..>9311)
                     /locus_tag="ACHGNS_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFN6938428.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3050 a         1604 c         1967 g         2690 t
ORIGIN      
        1 tgatatttct gtaaattaaa taaagtagtg aaaaataaga atcagtgctc taactgttca
       61 agaggatcaa gtggttcaaa tgtttctgta gtctgttttg cttccaattg gggcaaaaca
      121 gatccaattt ccagatactt cactttattc aatgtataag atagaataga acagctaagt
      181 aatgtataca gaaaatgtgt gagatccaaa taaagcaagg agacaactaa aataccaaaa
      241 gtaatcaaga aaagactttt gccaatctta atatgaaaat atttttcaat ggcttgataa
      301 atgatatctg ttccgcctgt gcttccgccg aatcgaaaga cgagaccgca accaatgcca
      361 gaaagaatac ctgtcatcaa actattgatc agtagactat ctacagcaaa atgtatatga
      421 aggctctccc ataaatgtaa aaagaaaact agcgcaaatg ttccgacgat cgtccggaga
      481 aaagcgtgtt ttccaaaaag aaacaatgag ataagcaata aaggtaaatt aaaaagcagt
      541 gaagtgacag aaggagcgaa tccaaaaaga ttatgcaaaa gcaaagcaat accagtaacc
      601 ccaccatctg ccagcttaga tggaatattg attcctgtca cagctaatcc gtagattcct
      661 gcgcctaaaa tgattccgca caattctgta aataaattta attttttcat ttctagcctc
      721 ttttcttttg aaaattttca gcaacttctt tattatactg acttatactc ttacttcaag
      781 aggaaaatga aaaaaaatta tttttggctg tttcttttta tttatcaaat aaggagtgga
      841 aaaatgcgag cagcagactt attgacaatt cttacaaaat cacccaaaaa aacagagtat
      901 caaggagttt ttttggctca agaacaaaaa ctggtgcctg tgacaatggt ccagataaat
      961 aaagcaaatc aatttatctt tttatttgaa cacggcaaag caccacttgc tatgaaagac
     1021 atctttcttt ttttgatgaa gaaccgtgag aaagctgttt attatcagtc aaaagaagaa
     1081 ctcattcctc tttatggagt gaaagagata gaaaataaag tggtgatata agtaaaaata
     1141 agaaaacaag acgtttatga tgtcaaaaca accatttttt acatttttgg caaaaaagtg
     1201 tcatgataca gtataatatt cgttgaacag gccaataaag gtgttctccc ccacgagatt
     1261 tctactttat tggctgtttc tttttattaa aataaaagtt gtgtgtgtaa aaaagatata
     1321 gctgattcca agtgacaatt tcgtatagat ttttatggta ttcgtagtga aaaaacgaga
     1381 accaaaaagt tgatccgact ttgttgcttg gattcttttt ttgtcagcat atttagaggt
     1441 atttttttct tttttttgtt cgtgctgtaa ggttaaggca agacaagtta gttgggaaaa
     1501 aggaaagaaa tgtttatact taaagaataa cagaaaggaa ggtcgcatga ataattttat
     1561 acaaacatta agtgaaagaa aaggcgagtt gctggaagct gcatttcaac atctgtccat
     1621 ttctctcatt tctttgctga ttgcagcact gattgctatt cctttagcga tttgggcagc
     1681 caatcataaa aaaatggcag aagtcttatt gcagatcacg ggtgtgctcc aaacgattcc
     1741 ttcacttgcc ttattaggtt tacttatccc atttgtaggg atcggaactg ttccagcatt
     1801 gattgcactt gttatttacg ctttgcttcc gattttccaa aacacgtata ttggtctagc
     1861 tgaaattgac ccgtctattg aagaagcagc ggtagcattt ggtatgtcca gaatgagacg
     1921 cttgctgaaa gtagagcttc ccatcgcatt acctgtcatt atctcaggta tccgaactgc
     1981 cttagtttta atcatcggta cggctacttt ggcagcattg atcggagcag gtggattagg
     2041 gacatttatt ttgttaggta ttgaccgaaa tacacctgtt ttgacattga ttggtgcgat
     2101 cagttctgcc ttgcttgcaa ttatattcag cggactgatt cgctggttgc agcataagaa
     2161 acctcgatat tccatcatta ctttagcagc agtggtgtgt ttatttggcg gcgtttcttt
     2221 atatcagtct gccttgttta aaaatgaaac catcacgatt gctggaaaat taggagcgga
     2281 acctgaaatc ctgatccaaa tgtataaaga attgattgaa gacaacacaa aaacagaagt
     2341 tgaattaaaa ccgaattttg gcaagacgag ttttttgttc aatgcactgg atcaaggtca
     2401 aatcgatatc tatcctgagt tcacaggtac tgtcctagaa agcttggtga aggtacctga
     2461 agcaatgaaa agtgctgatt tgacaaaaga aaaaacatat gacgaagcag aagaattatt
     2521 gaataagcaa ttcgatatga cattattaaa accaatggct taccaaaata cgtatgcctt
     2581 ggcagtcaag agatcttttg cgaacgaaca tcaacttaaa aagatttccg atttggcagc
     2641 tatctctgat cagatcaaag cgggattcac actggaattt attgatcgtt cagatggata
     2701 taaaggaatc caagaagcat atggcttgaa tttttcaagt gtacaaagca tggagccaag
     2761 tttgcgttac caagcaatca ataatggaga tgtcaatatt gtggatgcct attccacaga
     2821 tagccagctc aaggagtacg atctagtgac tctagaagat gatcgagggt tattcccagc
     2881 ttatcaaggc gcaccgctga tgaaaaaaga ttttgctaaa gaacatcctg aaatcgttaa
     2941 agcactgaat aaattaagcg gaaaaataac agaggatcaa atgattgata tgaattacca
     3001 agtcaatgtc gaaaagaaaa agccgacaga agtagcacga atcttcctga aaaatgaagg
     3061 attagtaaag gaggaagatc agtgactaaa ttaatcgagt tcaaacatgt gcaaaaaaag
     3121 tatgatggaa agtatgtcat cgatgaccta aatctggcaa tcaaaaaagg agaaatcttt
     3181 gttttggttg gtccttccgg tagtggaaaa acaactactt tgaaaatgat caatggatta
     3241 tctaaaccat cagcaggaga catctacttc aaaggaaaat cgttaaatga atataatctg
     3301 cagaaaatgc ggtggaatat gggatatgtg ctgcagcaaa tcgctttgtt cccgaccatg
     3361 acagtcaagc aaaatattga agtcatccct gaaatgttag gttgggaaaa gcagaaaaga
     3421 gcagaccgtg tagatgaact tcttcaaaaa gttggcttat ctcctgatat ttatcgagat
     3481 cgtatgccta gagagttgtc tggaggagaa cagcaacgga tcgggatcat ccgagctatt
     3541 gctgcaagcc cagatgtgat tttgatggac gagccattta gcgcattaga tccgatttca
     3601 agaaactcat tacaagaatt ggttttgtcc ttacacgaag aattgggcac gaccatcgtt
     3661 ttcgttacgc acaatatgga agaagcaatc aagctaggtg atcgtattgc atttatgaag
     3721 gatggagaaa ttatccaatg tgacacacca gagcagttat tgatgaatcc taaaaacgac
     3781 tacgttcggc atttctttga tgaaccgaaa cagacgaaag agtggcgagt agaagattta
     3841 gtcgtcaatg gatatttctt aaatgaaata ccagaaaatg ctcgtcaaca agtttgcttt
     3901 gatacgccta tgaaagaagt atattcgctt ctttcggtgt atccaagcat tgtgatagta
     3961 gaaaagcaaa gaagtattgg gtcgctgaca agtaaagaaa tctttgcttt tttaagtcga
     4021 gaggaggaag gaaatgaaaa catatgatgc aatcatcatc ggttcaggtg tgagcggctt
     4081 aagtgctgct tatggattga aagaagcagg taaaacagta ctagtggtcg aagaagatct
     4141 atggggcggg acttgtccta accgtggatg tgatcctaaa aaagtcttgt taagtgcagt
     4201 agaggcacgt aatcgggtaa aacaattgag tggaaaaggt tttaacgaga tcccaactgc
     4261 caactgggaa gaacttcaaa aattcaaacg aacatttacg gatccagtcc cagaaagccg
     4321 aaaaaaacaa ttagcagaag ctgaaatcga tcatctttca ggaaccgctc gttttttgga
     4381 tgattcatct attgaagtca acgaagaagt cttccatgcg gattacttag ttttggcaac
     4441 cgggcaaaga cctacgatct taccagtgga gggaaaggaa tacttgaaaa ctagtgcaga
     4501 ctttttatca ttgcctgtac tgcctaaaga aatcattttt atcggtggtg gttatattgc
     4561 ttttgagcta gctacgattg ccaatgctgc agggagcaaa gtaaccattg tccatcataa
     4621 tcaacggcca ttaaaagaat ttgaggcgtc tttggtagag gaagccgtcc atcaaatgga
     4681 agcatctggc attcagtttg cttttggtgt tgaaacccaa aagatcattt cagaaggtac
     4741 tcgctatcgt ttagtcggaa aagagacgga attagtagca gatatgattt tttgtgcgac
     4801 tgggagacag ccgaatacag aatctttagc acttgagcaa gcgaatatcg tctttgataa
     4861 acacggaatt gcagtcaatg attatctgca aacgagcaat ccaaaaattt ttgcgtgtgg
     4921 agatattgtt tcaaggaaaa ccccaaaatt gacaccagtc gccacttttg aaggaaatta
     4981 tgtagctaag cggataacgg atgcaacaag cgaacctatc aaatatccga ttatacctac
     5041 gatcgtctac gccagtccta aacttgctga agtaggagtt acaaaaagtc acgcctcttc
     5101 gtcagatcaa gtggtagaga tggatcttac cagttggttc acctatcatc gtgtcaatga
     5161 accagttgct aaggctgaat tgacctttga ccagcaaaat tatttgattg gtgctgcagt
     5221 gattagcgag caagcagacg aattgatcga tgatttgaca ctcgtcatca atcaaaaatt
     5281 gactaaaaaa gagttggata gctatatcat ggggtatccg acattggcaa gtgatctctc
     5341 ctatttattg aaataatcag catgataact aaagcgcttg ttgctttttc cttcttgtgc
     5401 tacactttaa aaaatgaata cctgtttctg gggatgtcgt agacgacatg agagtaagtt
     5461 gtgaacttag acccattgaa cctgtcagtt aatactggtg aagggagaaa catagcagac
     5521 gcttgaagcg ggttactatg tcgtgcccat ttcaagcgtt ttttgttatt catattagaa
     5581 gaaaggaggt aacaaaatga agaaaaatct ttatccagca gtcagtatgg cattcatttt
     5641 aattatttgg cagattgttg tcatagtttg gaaaatacct gcctttattt tacccggacc
     5701 tgctgctgta ttttctgcat tagcggcaga tgcacaagta ttactttccc attctgtcac
     5761 gacattaaat gaagcagtgc ttggtttggt aatagctagc gttctttctt ttattactgc
     5821 cttggcaatg gattattgga agtttttaga aatgtctttt tatccgttac tagtgatttc
     5881 acaaatgttg ccaatcatgg tgttagggcc attacttacc ctatggtttg gtttcggtat
     5941 gcttccaaaa gtgatccttg ttgtcttgat aagctatttt ccgatcgtag tttctttttc
     6001 ggatacattg aggaaagtat caaaagagca aatcatcttt ttgaaaacga tgggggcaga
     6061 tacagggaag atttatcgaa tatataaaat accagcgggt ttatccggct tcttttccgg
     6121 tttaaaagtg gctgctactt attgtgtcag cggagcagtc gttggagaat ggctaagcgc
     6181 tcaagcaggc ttagggtatt acatgatacg cgtaaaaaac agctaccaga tcgataaagt
     6241 ttttgctgcg atcatttgtg tcattctatt gagtttattg ctgaatggcg cttgttcatt
     6301 attgaaaaaa gggtattatc gtatgcttta tattttttaa gagaagggga tcaaatgaaa
     6361 aaattatggc ttttattacc gctactgttg cttttatccg cttgtggcac tgcaaaagaa
     6421 acaagtagca aacaagaaat aaaggatttg aaaaaagtga cacttgtttt ggattatgta
     6481 ccgaatacta atcatactgg catctaccta gcaaaagaaa aaggctatta taaagaggct
     6541 ggtttaaatg tccaaatcat cgaaccagga gataattcga caagtattgg tctagtaggt
     6601 gcggacaaag cacaatttgg tgtaagttat caagaagatg tcacttatgc tcacgcagat
     6661 ggacagaata taccagtcaa agcaattgct acagtgatca aacataatac ttctggattc
     6721 gccaccctat cagacagtaa catccattca ccaaaagatt ttgaaggaaa aacatatgca
     6781 ggttggcaaa gtccaagtga agaagctgtc ttaaaagctg tgatggaaaa agacggcggg
     6841 gacttttcaa aactgacaat ggtaggaagc aatggagaag ggccagaaag cttaggaaaa
     6901 tcctcagata tccaatggta ctttgaaggt tgggatatga tcaaagcaaa agaagcgggt
     6961 attgaactaa actatatccc attgaaagaa ttagatgaac ggttagatta ctatactccg
     7021 gtaattatta cgaatgatca actgatcaaa agtgatccag aattggtaca gtcttttatg
     7081 gatgccacga aaaaaggcta tcaggaagct attaaggatc caaatgacag tgctaaactg
     7141 ttgcaaaaat atgcaaaaga aaatgatcgg acgtttttag aagaatcaca agcattcttg
     7201 tcaaaaaatt atacggatga tcctgagaat tggggattga tggaagaaaa agtttggcgc
     7261 aattatacag cttttatgca ggaaaacgga ttgatcaagc aagatgtacc ttcgcaagaa
     7321 ctattcacta atcagtttat aaaataggag tgatagatat gtcaatccaa atcagccact
     7381 tgaatgtagt cattgaaaac ctgaaaatat tagaagacat ccatttcact atccctaaag
     7441 atacctttac atcgattgtc gcgccaagcg gagccggtaa gtcaacattg ctgaaaactt
     7501 taacaggagt ccagcctatg acttctggaa cgatcaaagt agacgatcaa aaagtaactg
     7561 gtctccatac agcatttagt tatatgccgc aagaagatat gttacttcct tggctgagcg
     7621 tctatcaaaa cgttacgtta tatcaaaaaa tcaatcatct aacaatcaat gaagaacaag
     7681 taagggatta tctggatatc tttgggttaa acggctatga acattttttg ccggaacaat
     7741 tatctggcgg gatgaagcag cgaacggcct tgttacgaac aatcatgaat ccttcttctt
     7801 acttgcttct tgatgaacca tttggagcgt tagatgctat gaccagaggg cagatgcaag
     7861 actggatgtt gaagctgcct aaaaaagcaa agcggactac tttacttgtg acgcatgata
     7921 tcgaagaagc aatttattta tcagacagga ttcttgtgct ttccgcccgt ccggcacatg
     7981 tgattgctga gattcaagtg cctgaaaaac gacggtcacg tgaatggcta ctgcagcaat
     8041 cagagttgaa gcaaacgatt tatcagttat tggcaggtga attagatgtt aagtgatgct
     8101 cattgtcatt tagatgggag tcagagttta gcactccttc agcaggaaca cacgattttg
     8161 acgatcatca attgtgattc gccagaagaa tggaaggaaa acagacagct tgcagcaagt
     8221 aagactcaag ccttaagtta cggtatccat ccttggaaag cagatagcta tacgtttgaa
     8281 caagttgagc cttttttgaa aaaagcaagg atcatcggtg aaatagggtt agataatatt
     8341 tggacgaatg taccgatgac tacacaaaaa aaagtatttg agcgacagct tgcttttgca
     8401 gccataaatg agaaacctgt tgtactgcac acaaaaggat gcgaaaaaga gatcttaaat
     8461 tatatccaag cttaccctaa tcactatttg atccattggt attcttcttt ggactatcaa
     8521 caagactata ttgatttagg ctgttatttc agcattggtc ttgatttgaa aaagaatcca
     8581 gcagtctggc aactagctaa agcagtccca atcgaccact tacttattga aacggatgga
     8641 ctacatgcag cagagtgggc tttagggggg attttttcag aaaaagatta tgtctcactg
     8701 cttgaagaac agttaaagac aatcgctgag atcaaagagc tatcagttga agaagttaaa
     8761 aaaaatactt atcaaaactt ggaacgtttc attagattag gtaaataaaa aagagatgtg
     8821 acacaactag tgtcgcacct ctttttggct ttctttgtat aaaagtaaac tgtttgatta
     8881 ataaatgaat cttagtacca accgtttgct tcccagaatg ctttcgccgc ttcccaagaa
     8941 ccataacgtg aagtaacata ttgttctgct actctttctt ggtttgcagc agagtagtca
     9001 ccatttaagt aagaagaatc taattggtaa cgtccgatgt aacgtccgtt cgttgctgtg
     9061 taagaaccgc ttgattcttt ttgtgcgatc cattcttttg cagagcttgt gcttgctgga
     9121 gtagcagcag tttctgttac agcaggagca ggttgtgttt cagcaacagg cgctgcttgt
     9181 ttttccgttt gaaccgtttc ttgtacaaca ggttgttcag cttggactgg tgcttcttga
     9241 acggctgctt gaggttcagc agctgcttgt gcagagcctt ctgttgggat tgttaattgt
     9301 tgtcctgagt a
//
LOCUS       DAZBTF010000094         9018 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000094 DAZBTF010000000
VERSION     DAZBTF010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.095"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1101
                     /locus_tag="ACHGNS_002534"
     CDS             <1..1101
                     /locus_tag="ACHGNS_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFN6938429.1"
                     /translation="KKETIVRFEQYNHMPLVTRLKKQRYTCKNCRTHWTAQSYFVRLS
                     HSIADHVRYKIASLLTEKVSLSFIAKSCQVSLTTVTRTLKEFKSYLPKPSKKILPRVL
                     MVDEFRSHASIEDKMSFICADGETGNLIAVLPTRKLPRLTSYFLGCTNPEEVEFLVTD
                     MNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQALNELRIREMNELRKAGQKSQAEKLKK
                     NWRFLLKNRASINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSAPLKEAYPFFHELV
                     EAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITSAMIYPYSNGKIEAKNT
                     HIKTMKRVSYGFKSFENMRIRIFLINQLINVR"
     gene            complement(1280..1615)
                     /locus_tag="ACHGNS_002535"
     CDS             complement(1280..1615)
                     /locus_tag="ACHGNS_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFN6938430.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(1599..1862)
                     /locus_tag="ACHGNS_002536"
     CDS             complement(1599..1862)
                     /locus_tag="ACHGNS_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFN6938431.1"
                     /translation="MAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQGKI
                     PFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2322..3125)
                     /locus_tag="ACHGNS_002537"
     CDS             complement(2322..3125)
                     /locus_tag="ACHGNS_002537"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFN6938432.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3103..4227)
                     /locus_tag="ACHGNS_002538"
     CDS             complement(3103..4227)
                     /locus_tag="ACHGNS_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6938433.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4243..5058)
                     /locus_tag="ACHGNS_002539"
     CDS             complement(4243..5058)
                     /locus_tag="ACHGNS_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6938434.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5048..5920)
                     /locus_tag="ACHGNS_002540"
     CDS             complement(5048..5920)
                     /locus_tag="ACHGNS_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6938435.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6005..7294)
                     /locus_tag="ACHGNS_002541"
     CDS             complement(6005..7294)
                     /locus_tag="ACHGNS_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN6938436.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7438..7950)
                     /locus_tag="ACHGNS_002542"
     CDS             complement(7438..7950)
                     /locus_tag="ACHGNS_002542"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938437.1"
                     /translation="MTINCDLKEEGLEMAVFSLAQTYQIWPQIVLSGYVQLSWAAIWG
                     KHIFYNIENQFEGKKLGDITEADLIDTLQKLAYQSVQIVNIHEGWLTKLAREVGQQLG
                     LTFSVWTVNELETIEYLARCGVYNITSRCAWAYLWKKGAQNKLFYNQNRGVKRFGDGI
                     HDHMGALFLA"
     gene            complement(8017..8166)
                     /locus_tag="ACHGNS_002543"
     CDS             complement(8017..8166)
                     /locus_tag="ACHGNS_002543"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFN6938438.1"
                     /translation="MTMITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLI
                     LAHDR"
     gene            complement(8643..>9018)
                     /locus_tag="ACHGNS_002544"
     CDS             complement(8643..>9018)
                     /locus_tag="ACHGNS_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN6938439.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSLEQLLSTVWLSVRTAFFVG"
BASE COUNT         2778 a         1933 c         1427 g         2880 t
ORIGIN      
        1 aagaaggaaa cgattgtccg ttttgaacaa tacaatcata tgcctttggt tacgcgccta
       61 aaaaagcaac gctatacctg taaaaactgt cgtacccatt ggacggctca aagttatttt
      121 gtccgactca gccattcaat cgcagatcat gttcgatata aaattgcttc tttactgact
      181 gaaaaagtat ctttgtcttt tattgcgaaa agttgtcagg tatctttgac taccgttact
      241 cgtacattga aagagtttaa aagctattta ccaaagcctt ctaagaagat tctccccaga
      301 gtattgatgg ttgatgaatt tcgttcgcac gcttccatag aagataaaat gagctttatt
      361 tgcgcagatg gtgaaacagg aaacttaatt gctgtcttgc ctacgcgtaa attacctcga
      421 ttaacaagct atttcttagg ctgcaccaat ccagaagaag tagaattctt agtcacagac
      481 atgaacgccg cttacttcca gcttaccaaa cgtgttctgc caaatgcgaa agtagtgatt
      541 gatcggtttc atattgtcaa acacatgaat caggcactca atgagttgcg tatccgtgaa
      601 atgaatgagc ttcgtaaagc aggacagaaa agtcaggcag aaaaactgaa aaagaactgg
      661 cgctttctgc taaaaaatcg tgcaagcatc aaccattatg agtacaaaac atggaaaagt
      721 ttccgagcac caaaataccc atttctcact gaagcaatga tgattgatcg attgcttgag
      781 ttttctgcgc ctctaaagga ggcgtatccc ttttttcatg aattagtcga agcctttcga
      841 gacaaagacc ctgacttatt tttctcctta ttggcagaac ttcccgaaac gttagatgac
      901 ggctttcggg agaagcttca aaaccttctg acctatgaag aaggtatcac cagcgcaatg
      961 atctatcctt attccaatgg aaaaattgaa gcgaagaata cccacataaa gacaatgaaa
     1021 cgagtatcct acggatttaa atcattcgag aacatgagaa ttagaatctt tttgatcaat
     1081 caattaatca atgtaagata acaaaaaatc tgaaccagaa tgtggttcat tctgacccgg
     1141 atttgatttt gcacaactca tcagtcctta ttgacaaaga gccaaaaatc tgagccagaa
     1201 tgaggttcat tctgacccag atttgatttt gtacaaccca tcagtcctta ttgacaaaga
     1261 gcccttcttt ttagtattat tataattctt ccatgcgttc ttccagtcgc tgtaaatcgc
     1321 ttggacttaa ttccccaatt tttttccatt tgaccttttt gttgtttttg ggtaattcgt
     1381 ttagagttcc tacgtcaatc caagattctt tttttagtcc tgcctgtttc caatccctta
     1441 tttcaacgta tttcagcttt attttatcgg atttagtatg atattttgaa gtaattgaca
     1501 gaaacgaaat tgtgtccagt ccatttttta gaatatagac aggccggcgt ttaccgccct
     1561 ctgcagctgt gaaagcaacg tagacggttg ctatatcatt agtattcatc ttcattgaac
     1621 cattcttcaa ttttttcgtc agtgtctagt ttttggacag gcaatttttc cgctaatctt
     1681 tgaatattat ggattcgttt acttttttcc gacaattcaa tagaaaatgg gatttttcct
     1741 tgctcaacaa tttgcttata aaaaacggtg acggctgttt tggggctcat gcctagttca
     1801 tcaataatgc tttctgcttc atttttcaat ttttcgtcca tagaaatttg taacttagcc
     1861 attttctcca cctctttctg acaaacacat tatatcacgg aaagagttca aatggaatta
     1921 atacaactac ttttgaactc cttgaaatgc agtgtattta tctaaatata aggaaaggat
     1981 atagtgctaa atggccttga tagatagaaa agtttcaaaa tccgccacag cgataattga
     2041 agcgaacact caacgacaca cagaacagga aaagactcct aaaagaacca aattaactag
     2101 gacgtgtctg aaaactccac aaagatgtgg tatgctctgg taaaaaacga atggcacaac
     2161 cacgaagacc gatatggttc ttggaaaact ttctccaccc tttttgtaat tggagggata
     2221 gtggtttatt gatagcgatt ttccaagttc ttcaagtgga accagacatt gagcaccacg
     2281 gctaaaaagt gaatgaagtc tttattttta atcaataatt tttaatgatt accagtaatt
     2341 ggttttttca tgcgccctat ttctatttga tgtggagtta aataatgctg ataagtaaaa
     2401 atttttccgt caattaaact gccactagtg aatcctgttc tattggtaaa aagagaatag
     2461 tctgggtatt catctacacc aaatgataaa gattcagcca taaactgttt tttaccagca
     2521 aattcagcac tgctaccttg atggatatgt cctacaaaaa tacctaaaat atcactttcg
     2581 gcaatcaatt cagcaaaacc ttccggaact tcagttacaa tagatggatc ttcccacgac
     2641 aatggatgat gaaccaataa aatcgttccc ttttcagcag gtgtacccaa ccactcttgt
     2701 aattgaatca tctgtcctat cgatattttt ccttcgtgct gggtatcttg agcagaatcc
     2761 aaggaaataa ttcgtaaccc attttgattc gtacaatgga tatactctcc cgtttcattg
     2821 ctttcaacac tcattccttc aaagaaaaca ctgcgacgat catgattccc acgacaaaaa
     2881 tagtaaggtg tggttgggaa atattgatcc aacattttcc gtaagacttg ataatcagaa
     2941 atcccccctt catgtactaa atcacctgtt aatagaataa aatcgtacac tgttaacgag
     3001 agttcttgta acaatttttc caatgccaac tgtggatttt taattgttgg aaatagttca
     3061 gcagtaaaca agttagtttg atagttttct ctaaaatgga tatcagagag atgcaaaaag
     3121 cgcatggcta ttccctcttt cttcaataaa ataatcagga tatcctaaat tttcttgcgt
     3181 ctgataatca aaaaaatgca ttctttcagg ttctatgcgg agattgactg catcaccaat
     3241 ctgccaagta ggtttttcat taaggacaat catttctata ccatcgactt taacggtgta
     3301 cgcataactt tgacctaata gctcacgata cttcacttga ccgtgtaaat cgcttgtttc
     3361 ttttgaaaag gttacgtgtt ctggtcttat gcctaaatac aatgagccac taacctgaag
     3421 aagttgtttt tgccaagctg gattcaatgt taaaaattga ctggctacct ttaatgttcc
     3481 ttgatcataa gtaactggca taatattcat gcctggcgaa ccaataaaat ttgcagtaaa
     3541 agtattttta ggtttcgtat aaacattttt cggcgtatca atcatctgaa ttctaccttc
     3601 attcaataaa acaatccggt cagctaacgt catcgcttca atttgatcat gagtgacgta
     3661 gacaaaagtt tgtttataac gttgatgaat ttttaccaat tcttttcgtg catccaatcg
     3721 taaacgcaca tccaaatttg ataacggttc atctaataaa aaataattac tccgtttaac
     3781 caccgcacga gccaatgcta cacgctgccg ttgccctcct gacaaatctt taggaagtcg
     3841 atcttggtat ggcgttaaac ctaacatctt caaagcttct tccaaacggt gctgaatgtc
     3901 agctgtctta attttattcg cccttaatgc aaaaataata ttctccttta cagtcatgtg
     3961 aggatataaa gcataatttt gaaaaaccat tgcgatctct cgttctccac aaggtagctt
     4021 atttgctagt tgtgtaccta aaaaaagctc tccactcgta attttttcca aaccagcaat
     4081 cattctcagc aaagtagatt ttcctgaacc agatggtccc agtaacacaa ttcgttctcc
     4141 agcggccaca gctaaatcaa tttcttttaa aatggtatgt tctccatatt tttttccgat
     4201 atttcttaat tcaagcgttt ctcccatgat ttgtcctcca atttatttaa tacctgcaga
     4261 aacaaaagtc ccgataatat atttttgaaa aattgcatac aagactaacg gtacaaacat
     4321 tgtaattaaa ctaatcgcca ttgctacggt aaaatttgtc ccaccttcag aactaatata
     4381 ggtctgcatc gccaacggca aagtataatt atctactgtc cgtaaaatta ataccggcca
     4441 aacatactca ttccaagagg taataaaaaa ccaaataccg gtagaagtaa tcgcatgtct
     4501 agtaagagga aaaacaattt tactcatgac ggttgtttgt ttgatgttat ccaatgccgc
     4561 cacatctaga aggctcactg gaatttcccg cattgtttgc gtcaataaaa aaatgcccat
     4621 cgcatcagca aattgtggta ttatcacacc ccaagtagta tcaattaaat taagttttgc
     4681 aatcaacaaa taattaggaa tcatcgcaac agtaaaagga atgaacatcg taccaatgat
     4741 gataaagtac cagattttct tccctttgat tcggcaataa gtaaaactat atgccgcaaa
     4801 aaaagctacg cctaacttaa caaccgttat aattgtagca ataaaaaaag tatttctcgt
     4861 aattttgata attggaagat tttcgaacaa atagcaaaaa ttttccaagg taaacggctg
     4921 tggtatcaaa ctagtaatcg tctgataggc atctttcaat ggcttaaaag aattggccat
     4981 aataaacaat aatggataga taataatgat tatgatgaac ataaataaga gatgtggcaa
     5041 ccagcgttta attttcatag taaaccccct tttccacaaa tttaaatatt acccctaaga
     5101 aaacagcaaa tattaataaa gttaaaactg aaattgctga agattctccg actttataca
     5161 tttcaaaagc cttttgataa gcataataga tcacgtttgc actaccatta ttcggtccac
     5221 cacgagtgat gatcttaatc ggagtaaaca catactgcag accttgaaca atggtcatca
     5281 ccaataaata tagtcctgtc gaactactca taggcaacac aattgtaaaa aaaatgcgcc
     5341 agatgggtac acgatccaac ttagctgctt cgatcatctc ttgatcaatt cccacaattg
     5401 ctgcaaaaat gacaatgaaa ttataaccaa aaactttcca agcagtaata cctgcaatca
     5461 cagcaattac ccaaccttgt aaattcatcc acataggcag tcttaaacca aaatttccca
     5521 acaagacagc aattggtccc gataccggat ttaatatcca tgtaaaaacc atccccgcca
     5581 ctaccaaaga aatcacagct ggaataaaaa ataatggttt atacaaattc tgcccttttt
     5641 ctataactat tcctaataaa aaggatgtaa tatatgggaa cacacagctc agcaagacaa
     5701 atagaacggt ataggacaaa gtattcttca acactttatg aaagactgga tcagtcagca
     5761 cctttacata gttggcaaag ccaacaaatt ctttagtggg ggccaccata ttccagtcaa
     5821 aaaaactcaa atagatcgta taaatcaatg gataaaaaac aaacacgccc aacaaaataa
     5881 ttgctggcgc cagatacaaa taacctacat agcgcttcat ttccgacagc ctcctcatcc
     5941 ttatgtcaaa agcagacgac cttgtttttc ctttcttaca ggcgctgtcg tctgcttgct
     6001 ttgcttattt gagtaactca ttaatttcct tttgagtagc tttcatcgtt tcattgacgg
     6061 aattacctga taaaatttta tcccgcatat ccaacaattt ttgttcagct tctaatccag
     6121 catctccagg aaatgaactc caactgacca tcttatccat ctgatcaatt gctggtatca
     6181 tcatctgatt ttcagctaag aaagctttta atccattttg ggcgtctgca acatctttac
     6241 gtggtggtaa ataaccagta ccttctgtcc atttagctaa agattcaaca ctataaagat
     6301 attttaagaa atcccaagtt gcttcctttt tcttatccgt ttgtgatgta acagctaaca
     6361 ttgcgccacc agcaggtagt ttaacttttt tcccttccca acttggtgac tcaactgctt
     6421 tagcagtaaa agtactattt ttttgaatgt tgtccctttg agcaatagtg gtatagacca
     6481 tcgcgacgtt accatcaata aagctttgca caccttgttc ccacgtagtg tgtaatgccg
     6541 tctggtcttt taccatcctt tgatataatt cataagcagc gataccatct ttagaagcaa
     6601 aggtagcttt gccatctttt attacacttg ccccattact ttctaaaatt gcttgttgtg
     6661 cccaattatc agcaggttct tgaatataca cgccatactt tccagttttt tcttttatta
     6721 tcttagaaaa agtttgaact tcttcccaag tttttggacc attttcatcc aagcccgctg
     6781 cttttaatat atcttgattt aaatataaaa ccggattact aatggagtaa ggaataccca
     6841 cttgtttacc atctttttca gacagtgcca tgatattgtc taaaaaatga tctttaaaat
     6901 aatcagcatt atccggatcc acttcttcta ctaaagattg aggattagta tatttgaaat
     6961 tttctgcaaa ataatcttta aaagcccatc ccacttgtac tacgtccggt atttctttgg
     7021 aggcaacaga agcttgtaaa ttttgcatca agcctttata catatcagga ttatattttg
     7081 ctacaacctt cactttgtct tgagattgat tgtatttatc tactaattca ttcactgttt
     7141 ttccacctag ggtatccgca ttaacatgcc aatactctat cgttacacga ccctccgatt
     7201 tttttgttgt cttggtattc ccactaccac aagctgccaa agagatgaca cttaaaacag
     7261 acacaacccc tataacaaga tttttcttta acataacatt aacttcctcc taatttcttt
     7321 atggccattt ttctaatcaa gaaatattat gaactaccaa tgtaaatttc tagttaattt
     7381 tctgtaaata attcgaaatt tgtgcatttc ggggtacagg gtcactggac atatccatca
     7441 agctaagaat aaagccccca tatggtcatg tatcccatct ccaaatctct taactcctct
     7501 gttttgatta taaaatagtt tattctgtgc tcctttcttc cataaatagg cccatgcaca
     7561 acgtgaggtt atattataaa caccacagcg cgcaaggtac tcaatcgttt ctagttcatt
     7621 gactgtccat acagaaaatg tcaatcctag ctgctgcccc acttcacggg ccaacttagt
     7681 taaccatcct tcatgaatat tgacgatttg aacgctttga taggctaatt tctgtaaggt
     7741 atcgatcaaa tctgcttcgg taatatcccc aagttttttc ccttcgaact gattttcaat
     7801 attataaaat atatgctttc cccaaatcgc agcccaagat aattgtacat agccactaag
     7861 aacaatttgt ggccagattt gataggtttg tgccagcgaa aaaacggcca tttccaatcc
     7921 ctcttctttt aaatcacagt taatggtcat ggttgccttc tcattggcca ctgcttgaaa
     7981 cacctgctca agggttaccg aatctgggac aacttcttac ctgtcatggg ctagtattag
     8041 cgtaccatct gtttgctgac gcacatctac ttcaaaacaa agctggcgat ccaaacaaaa
     8101 gcgaagaaat gcccagctat tctcctccgt attgtcacta ccagagtgtg cagtgatcat
     8161 ggtcattttg acccctcctt taattccgtg attttctccc acacgtccac tataccgatg
     8221 aagcgttaat ttcttgttaa tttcttgtaa attccctgcg ttttttcagt aaactagttg
     8281 taaatcttgc atgctccttc ctacgaactc atgaatcgtc gattttgccc ttctagctag
     8341 tttcaccaat taagtagttc tacattcccc gcttttaacc tactacacct ttttttgttt
     8401 tcagctattc cttcttcttg ctgaaagtcc ttgaggttct ttcttacagg ataccatcaa
     8461 ggtgttcaat ggcttattcc cattgaatga ccgagactta ctatcagatt tctgctcgtt
     8521 gaaccaagtc tttgatacag gatttcttcc gtcatcttct cacaaatacc accttgcgtt
     8581 tctctagcga ttggtcgatg tgcaccctcc cagtggactg tcactacccc tttttgctat
     8641 gcctagccca caaaaaaagc tgtcctcaca gacagccaga ctgtagacaa aagctgttcc
     8701 aaactcgtaa ctcgagcttg gaacagcttt ttatctactt cgtttcgaat ttttgaacaa
     8761 aaaaataaag gctccttgcc tttccatagc caggtggcta atttctttaa attcattgca
     8821 gcaaacacta gcatcgtatg cgtcgcgact ttttccaagc ctcgatactt ggtccaacgc
     8881 atcccatgct tttcttttgc atccgcaaat attcgttcaa tcgtttgttt ccgttttttg
     8941 taaattgatt tattcagatc tgttaggcga agatgttcca cttcatccat cagatctccc
     9001 caaacatgtc tcgtgatg
//
LOCUS       DAZBTF010000095         8944 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000095 DAZBTF010000000
VERSION     DAZBTF010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.096"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..1152)
                     /locus_tag="ACHGNS_002545"
     CDS             complement(13..1152)
                     /locus_tag="ACHGNS_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFN6938440.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1551..2849)
                     /gene="eno"
                     /locus_tag="ACHGNS_002546"
     CDS             complement(1551..2849)
                     /gene="eno"
                     /locus_tag="ACHGNS_002546"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFN6938441.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(2965..3720)
                     /gene="tpiA"
                     /locus_tag="ACHGNS_002547"
     CDS             complement(2965..3720)
                     /gene="tpiA"
                     /locus_tag="ACHGNS_002547"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFN6938442.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(3824..5014)
                     /gene="pgk"
                     /locus_tag="ACHGNS_002548"
     CDS             complement(3824..5014)
                     /gene="pgk"
                     /locus_tag="ACHGNS_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFN6938443.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(5124..6125)
                     /gene="gap"
                     /locus_tag="ACHGNS_002549"
     CDS             complement(5124..6125)
                     /gene="gap"
                     /locus_tag="ACHGNS_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFN6938444.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(6166..7257)
                     /locus_tag="ACHGNS_002550"
     CDS             complement(6166..7257)
                     /locus_tag="ACHGNS_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFN6938445.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(7406..8740)
                     /gene="rpoN"
                     /locus_tag="ACHGNS_002551"
     CDS             complement(7406..8740)
                     /gene="rpoN"
                     /locus_tag="ACHGNS_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFN6938446.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            8800..8871
                     /locus_tag="ACHGNS_002552"
     tRNA            8800..8871
                     /locus_tag="ACHGNS_002552"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8833..8835,aa:Arg,seq:ccg)
BASE COUNT         2320 a         1897 c         1648 g         3079 t
ORIGIN      
        1 attaaaaaaa tcttacgcta agtaggataa aataattcct tgatattttt cgatcatatc
       61 caaataatta tcgatttcaa catactcatc tacttgatga ggcaaagtaa cgacaccagg
      121 cccaaagacg acaaagtcga aggaatggtt agctttcgta aattcggctg catccgttgt
      181 tgctgccgct ccaactaaag gtaatggctg gctgaattgt tgctgaatgc aatgaatcaa
      241 aggagaatct ggatcagctt tgacaggtat tttgttgtaa tcgatcatca attccaaatg
      301 atagtctgtc tcttgattca attcattgac gattgattgc agcaatgcaa tgattttatc
      361 atttgggtat tctggaatcg atcgaatgtt cccttggagc tgcgcatggc ttgggatgct
      421 gttgacttga ttcccgccac tgatcaatgt aacattatgg attgtttttc ctaggacagg
      481 attctcgatt gtttcagcga ggtgattcat ttcagcattt gctttcgtaa tgaattcatt
      541 taggtgattg attgcattat aaccttggtc tggcatagag ctatgcgctt cttttccgtg
      601 cgaggtgact gtatagttga ttgatcccat gtgcgtatac atcaaactgt aattggttgg
      661 ctctccgatg atcaaggcat ctaagtcatc tacatagcct gctttcgtga gctgttcccc
      721 acctagttca cccacttctt ctccgacagt ggctaataat ttgactgttc cgttaaatgg
      781 ttttcctgac tctttcagtt cgatcatggc aatcaccatt gctgccaagc cactcttcat
      841 atctgtagca ccacgtccgt acaaacggtt cccatggatt tctgctgcaa agggtgcata
      901 ggtccaagac gattcatcac cagccgccac gacatccata tgcccagata ggcctaacac
      961 ttttcctgat tggccctttt ggtaacgagc aatcaaatta tctctaccat ctctgtaaga
     1021 aacaatctct cctgttatgt catgctttgc caaaagctta ttcaaatagg ctgctacttc
     1081 accttcgttt ccattcactg atttaattcg gataatctct tgtaaaatcg cgattttttc
     1141 ttcttttttc atctaatcct ctcccttgcc atttcttttt attgtatttg agtgtgctcg
     1201 aattttcaaa taggagcaac caaagtaaaa atgaagaaag agagccactt tatgaaaaat
     1261 atgctgctac ttccctccta ttttatttat acgagaggac ttgcccatag attcctattc
     1321 tactgttcta tccttaaaag ctcttatatg atgctagtta ttgttagctt tcctttttca
     1381 aggatgatct tttcttgtac cattctttgt aaacatcata tacagaagct agttgcaaaa
     1441 aaactctcta cctgctgata tttcagcaag tagagagttc gattacttaa ctaagtgatc
     1501 atactcgact atctttcaat agtcgatcat gtatcttcat tagaaaagaa ttatttgttt
     1561 tttaagttgt agaaagattt caaacctttg tattctgcaa cttcaccaag ttggtcttcg
     1621 atacgtagta attggttgta tttagcaata cggtcagtac gtgatagaga accagttttg
     1681 atttggccag cgtttgttgc aacagcgata tcagagattg ttgaatcttc tgtttcacca
     1741 gaacggtgag atacaactgc tgtgtagcca gcttctttag ccatttcgat agcttcgaaa
     1801 gtttctgtta atgtaccgat ttggttcact ttgataagga ttgagttagc aattcctttt
     1861 tcgatacctt cagataattt ttgagtgttt gttacgaaca agtcgtcacc aactaattga
     1921 actttgtcgc ctaatacgtc agttaatttc ttgaatccgt cccagtcgtt ttcgtctaat
     1981 ccatcttcga ttgagatgat tggatattta gaaactaatt cttcgtagaa tttgatcatt
     2041 tcgtcagttg ttttttcgcc ttcgcctgag tcagctagta cgtaaacacc tttttctttg
     2101 tcgtagaatt ctgaagaagc agcatccata gcaagaacaa cgtctttacc aggtacatag
     2161 ccagcttttt cgattgcttc gatgattact tcaaaacctt cttcgtttga accaaggttt
     2221 ggagcaaatc caccttcgtc accaacagaa gttgctaatc cgcgagcttt caagattgaa
     2281 gctaatgcgt ggaatacttc agcacccata cgtagagctt ctttgaatgt tggagcgcca
     2341 acaggcatga tcatgaattc ttggaagtcg attgagttat cagcatgtga tccgccgttg
     2401 atgatgttca tcattggtgt tggcaatact tttgtgttga atccgcctag gtagtggtat
     2461 aaaggtactt ctaggtagtc agcagctgca cgtgcaacag cgatagaaac acctaaaata
     2521 gcgttcgcac ctaatttacc tttgttagga gttccgtcta aagcgatcat agctttgtcg
     2581 atagccattt ggtcacgaac atcgtagcca atgattgctt cagcaatgat gttgtttacg
     2641 ttgtcgacag ctttagttac acctttaccg ccataacgag ctttgtctcc gtcacgtaat
     2701 tcaactgctt cgtattcacc agttgaagca cctgaaggaa ccattccgcg gccaaaagct
     2761 ccgctttctg tgtatacttc tacttcgatt gttgggttac cacgtgagtc taagatctcg
     2821 cgagcgtaaa catcagtaat aattgacatg ttttgtctct cctttgagtt tgttttttcc
     2881 taagggattt ccctcatata cgattttagt taattttgct cattagtgca aacatttctt
     2941 gcatttttcg caaattttca acaattattt tacagcgtct aacaatgaca agaatgaatc
     3001 tgcttgcaag cttgcgccac cgactaaagc accgtcaaca ttttcttttg ccatgtattc
     3061 agcaatattt tctggtttta cagaaccgcc gtattggata cgaactgctt ctgatacttc
     3121 ttttccataa agtttttcaa ctgtttgacg tacaactcca caaatttcgt cagcgatttg
     3181 tgcgtcagct gatttaccag taccgattgc ccagattggt tcataagcaa taaccatgtt
     3241 tgacacctgt tcagctgaaa gatcagctaa accagctgta atttgtcctt cgatccattc
     3301 agcagttttg cctgcttcat aggtttctaa agattcgccg cagcatagga ttggtgtcat
     3361 accatttgca aagatagctt tcgctttttt attgatgtct tcgtctgttt catggaaata
     3421 ttcacgacgt tctgaatgtc cgatgatcac ataatgaaca cctagatcag caagagctgc
     3481 tggtgaagtt tcaccagtga acgcacctga gttttcccag tagcagtttt gtgctgagat
     3541 ttttagttct gtaccttcag ctgatgtcat taattcttgt aagaataatg caggagatcc
     3601 gatgactgaa tcaactttgt catttgaagg gattttgttt tttactgctt cagcaaattc
     3661 ttttgcttct gatgcagttt tgttcatttt ccagttacct gcgataatag gtttacgcat
     3721 tgaggaacac actccttaat ttattgaaaa atggctaggg agtcatgaaa actcccttca
     3781 ccttttgttc gattactcgt cagaattgag caaacttggt cgtttattta tcgttgattg
     3841 ctgctagtcc tggtaattct ttaccttcaa gcaattccaa gcttgcgccg ccacctgttg
     3901 agatgtgtgt gaatttgtca gcaaatccta attgttctgc tgctgcggct gagtctccgc
     3961 caccgatgat tgttgttgcg ccttctaagt tagcgattgc ttcacaaaca ccgattgtac
     4021 ctttagcaaa gttactcatt tcaaatacac ccataggtcc gttccaaaca actgtttttg
     4081 caccttgtaa tgtatcagca aataatttta ctgatgctgg gccgatgtct aaagccatca
     4141 aaccatcagg aatgtctcct tcgattactt gtgtttcaac atcatttgaa aattctggtg
     4201 cacagacatt gtcgattggc aagacaagtt tgtcgcctgc tttttcgatt aattcttttg
     4261 ctagttccac tttatcagct tctactagag agttaccgat cttgattcct tttgcttcgt
     4321 agaatgtgta agtcattccg ccaccgatca agattttatc tgctttagga atcaagtttt
     4381 cgattacgcc gattttatca gaaacttttg cgccaccaag gattgcaatc attggtcgtt
     4441 ttggttcttc tactgcttca ccgatgaact tgatttcttt ttccatcaag aatccagcaa
     4501 ctgttgggat accagttgaa gcgataccta cgttagaagc atgtgcacgg tgagctgtac
     4561 cgaaagcatc gttaacaaat acatcgccta atgaagccca gtatttacct agttctgtat
     4621 catttttgct ttctttttta ccgtcaatat cttcaaatcg tgtattttcg aatacgacaa
     4681 cgtcgccgtc attcatgttg cggattgctt cttctaattg ttctccgcga gtttcaggta
     4741 cgaatgttac ttctttacct aataattcgc ctagacgttt tgcaactgga gctaaagatt
     4801 ttccttcttt atcttcttct gtttttacac gtccaaggtg agagaaaagg attgcttttc
     4861 cgccttgttc tagaacgtag ttgattgttg gcaatgctgc tttgatacga gtgtcgtcag
     4921 tgatcacgcc gtctttcaaa ggaacgttga agtcaacacg gacaagaact tttttgtctt
     4981 ttaaatcgat atctttcact gttttcttag ccattttatt tcctcctgaa taaacttatt
     5041 atcaaaaaaa gcggaagaag cgcgattgct tccccgcttt taaatgtaca tgtctataat
     5101 ttacacttgt acgtttgtct atcttataag ttagcgaagt attctaaagt acgtactaat
     5161 tgtgcagtgt atgacatttc gttgtcgtac caagcaacag ttttaactaa ttgtttgtcg
     5221 ccaactgtca ttactttagt ttgtgtagca tcaaataatg aaccgtaagt cataccaacg
     5281 atatctgaag aaacgatttc gtctgtgttg tatccgtaag attcgtttga agcttctttc
     5341 attgctgcat tgatttcgtc aacagttact tctttttcaa gaactgttac taattctgtt
     5401 aatgaacctg ttggtacagg aacacgttga gcagctccgt ctaatttacc gttcaattcg
     5461 ggaattacta aaccgatagc tttagcagca cctgttgagt taggaacgat gtttgcagca
     5521 gcagcgcgtg cacggcggaa gtcaccttta ggatgaggtc cgtctagagt catttggtca
     5581 cctgtgtaag cgtggatagt tgtcattaat ccttcaacaa caccaaattt gtcgttcaat
     5641 gttttagcca taggagctaa acagttagta gtacatgaag cacctgagat aactgtttct
     5701 ttaccagtta atgtttcgtg gtttgtgtta taaacgattg ttggaacatc gtttccgcca
     5761 ggagcagaga taacaacgcg tttagcacca gctgttaaat gtttttcagc tgctgattta
     5821 gaagtgaaga aaccagtaca ttctagaacg atatctacgc ctagttcgcc ccatggtaat
     5881 tcttctgggt tgcggttagc taatacttta acttcttttc cgtttacgtt gaatgaacct
     5941 tcatgaactt caactgttcc gttgaaacgt ccttgagttg tgtcgtattt taacaagtgt
     6001 gccaacattt tagcatctgt caagtcgttg attgctacta cttcgattcc atctacatct
     6061 tggatacgac ggaatgctag acgtccaata cgtccaaaac cattaatacc tactttaact
     6121 gtcatttaag atttcctcct tatgaaaatc aaaaaaatat ttattttaaa gggttacccc
     6181 ttttaaaatc tcatttgcag cagcttcatc agtgatgagc cacgtttgtt ttggtgcatt
     6241 tttcatataa gctcgaattg ctttagcttt tgattttccg cccgcgattg ccatgatgat
     6301 cgggactttt tccaaatctt gcaattctat gccgattcta gggaccttat aaacgacttc
     6361 acctttttca ttgaaaaagt agccaaaaga ttccgcaaca gcatttgctt gtttcagcat
     6421 gactaattct ttttcagtca tcttacgacg tgctgccatg tgtaatgcac gcccaatact
     6481 atgaataaca caattcgctt cactaatcaa gtttaagact tcttggatag atggctcatt
     6541 gagtaaagaa ttgtaagttg ccagacttaa ttgttccgga acataaagtg cgcggtggtt
     6601 accgtttgct ttagcagcca ttcttgcact gacagaattt gcctgtacag ttatcgcttc
     6661 accaatcccg cctcttgcag ggacaaacag gttatgccgt ttcttatttt ccagattaga
     6721 caattgttcg gctaccacag ccatagtagt tcctcccatc acagcgatga tattctcgcc
     6781 ttctggaagt agtcggtcta aggcttcgct cagcgcttcg ccgaatgcat cagccacttt
     6841 ggtctgctct tcgctgtttc ctgaaacaac gatacagcgt tggttaccaa aatattctgc
     6901 caactgttgt tcggtttgat gcgtacctaa aagctgatcc atgaagtttt caaggctttg
     6961 ataaacttct tcgcccttag ttgtcagcgt cataccgctt tttgaggtgt cgatcaaatt
     7021 cagatttttc aaaaggtctg tttctgttcg taagactcgt tctgtgatcc ccatactttc
     7081 cgataaactt cgacgcccaa tcggctgcat ccaatagata tttcgtaaaa ttttatagcg
     7141 ttcttgcaac acttcgataa tatctggtgc aacagattca atcattttta tttcatccag
     7201 catagcgatc tccttagttg gacactttac gtccaatgta gacttaaaac gacccatata
     7261 cgataaaaaa agaaattaac tattcgaatt tcacatcaat aaagggaaat ctcaacctca
     7321 cgactgtaag tctaacagtt ttgctctcgt ttttcaacta aaaagactag aaaaagtgaa
     7381 atactttttc tagtcttttg actgattagt tatcataacg ttttcttttt gatgaactcg
     7441 ggattcctaa taaatcacga tattttgcta ctgttcgtct tgagatcgct atctcatctt
     7501 ttttcagcaa ttcaaccaat ttctggtcag acaacggctt gtttttgtct tcttgatcga
     7561 ctaattcctg cagctttttc ttcgctgtat ctgctgaaag gtcttctgtc tgttctgtac
     7621 tattcgtagg aattcgtgta gtgaaaaatt tttttaattc aaatacaccg aagtctgttt
     7681 ccaaatattt cccgttgact gcacgactga ccgtggattc atgcacatct atctcagttg
     7741 caacttcctt taacgtaaga ggcttgattg gacgttcctt gtcgataaag aaagcttttt
     7801 gccgggaaac aatgacttga gcaacccgca aaattgtatc cccacgttgt aggatcgttt
     7861 tcttcaacca ttcgaattcc tgtttccgtt cttttaaata attttcagtt tctttatctg
     7921 cttgttgtga catttgtttg aagtaactct cttgaaagcg tatatttggc tgattttttc
     7981 ggtttgagat aacttgtaca tttttatcgt cgtccactaa tacccggaca tcaggaataa
     8041 tgtacaattc gctgctacgg tcgaaaatcc ggccagggct tggactcaac gtttgtaaat
     8101 agtcgaatat ctgctgaaca gcatgtagat caacagaaaa acgttgtgcg atttctttcc
     8161 attttcgctc caccagttcc tcaaaacatt cttccaacac gatataagct aattccggcg
     8221 cataatcatc tcgttctgtt tgaagcatga gacattcttg caaactacgt gcacctactc
     8281 cagctggttc taattgctga atcagagtca acgcatcgag catttggata ggcgtagctc
     8341 caatctcttt tccggcttct tctaaactaa tcgtcaaaaa tccgttcaaa tcgatgtatt
     8401 ctacaagata aagaacgatt ttcctcaaaa aagtatctcg gtaattcaaa tggacttgtt
     8461 cgatcaatga atcaaataaa gacccttttg tatcaggaat ctgatttagg taatttgtct
     8521 cttctccttc ataagaagat gaagaagctt ttatgtaatc tggctgccat tccggctcaa
     8581 caacttccac caatggattt tccattgctt gattctcaac gaatgccaac agctcctcac
     8641 tgttatattg taaaacttgg atggactgtt gcaactgttg cgtcatggct agtttctgtg
     8701 tctgctgctg tttttgtgaa aaattttgct caaatttcat ttttttgtcc aatccccttg
     8761 taatttttta tctgtttagt tacaattatc ccagtgcttg cgtccttagt gtagtggata
     8821 tcacgtaaga ttccggttct tgagacgggg gttcgattcc ctcaggacgc gttctactgc
     8881 tattttagca taatttacga atagttgctt ctttattttt aagtgaaaac tgtccgccaa
     8941 agaa
//
LOCUS       DAZBTF010000096         8804 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000096 DAZBTF010000000
VERSION     DAZBTF010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.097"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(402..2783)
                     /locus_tag="ACHGNS_002553"
     CDS             complement(402..2783)
                     /locus_tag="ACHGNS_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFN6938447.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2773..3420)
                     /gene="recU"
                     /locus_tag="ACHGNS_002554"
     CDS             complement(2773..3420)
                     /gene="recU"
                     /locus_tag="ACHGNS_002554"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFN6938448.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3484..4023
                     /locus_tag="ACHGNS_002555"
     CDS             3484..4023
                     /locus_tag="ACHGNS_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFN6938449.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4113..4532
                     /gene="gpsB"
                     /locus_tag="ACHGNS_002556"
     CDS             4113..4532
                     /gene="gpsB"
                     /locus_tag="ACHGNS_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFN6938450.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4554..4921
                     /gene="rnpB"
                     /locus_tag="ACHGNS_002557"
     ncRNA           4554..4921
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHGNS_002557"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5130..6296
                     /locus_tag="ACHGNS_002558"
     CDS             5130..6296
                     /locus_tag="ACHGNS_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFN6938451.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6342..7838
                     /locus_tag="ACHGNS_002559"
     CDS             6342..7838
                     /locus_tag="ACHGNS_002559"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFN6938452.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7919..8602
                     /locus_tag="ACHGNS_002560"
     CDS             7919..8602
                     /locus_tag="ACHGNS_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFN6938453.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8611..>8804
                     /locus_tag="ACHGNS_002561"
     CDS             8611..>8804
                     /locus_tag="ACHGNS_002561"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938454.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRL"
BASE COUNT         2783 a         1587 c         1857 g         2577 t
ORIGIN      
        1 tattgttttc gataaaaaaa gatggtgcag acaaattatg ataattaagc atactatcag
       61 ataactcaaa atgaagcctt ctttcaaaaa attattcaaa accatatatt gtatttttta
      121 tttgataata acaatgggat tattaagaag tggcactaaa taaaagacta gtgtgtattt
      181 agccgattta attataaaca agtaaatgga taaagtatat gtaatgataa aagaaaaatg
      241 gattttctaa tttttaagct aaatgttaga tcacacatgt atatgctgga tagtcttata
      301 aaaaatcatg taatacagct tggtacatga tttttatttg tagctgtttt ttacatagac
      361 acaaaaaaga aaccacgctt gtagacgtgg tttctttttt actatctgtt tccagaagct
      421 gaggtgctgc tggatggtgt atggtcattt gcagcatccg ctccgctagt tgctggctca
      481 ctggaagaag caggaggagc agatgaagac ggtgtcgagc tttcttcgga tgaagaagaa
      541 ggaaccgttg ttgaagacgc tggctcgctt gtttctgctg tcgtgctttc ttttgaggac
      601 tcagcagttg attcgctttg actagtagag ctagagcttt gagtcgttgt ctctgttgtt
      661 gagcttccag gagtctggac atagctggaa gatggaatag ttgttgatgg tgtaattgca
      721 ttcgatctag ctgtatattg atctttgtag tacagttcac cgcccacacg aattaatccg
      781 cttggcatct cccagtctgt atttgtcacg ttagctgaaa catactgcat aagctcgcga
      841 taaacatcag aggctacgtg tgacgattct gaagtgacag gcgtcatttt tttattgtaa
      901 cctgtccaca cggaaatcga ataatttggc gtatagcccg caaataggat atctggatac
      961 accccagaag aaataccaag ttttgcatac tcgtcatctg tgtaattgga tgttccggtc
     1021 ttaccggctt gatataaccc agcgatttgc gcattcgtac cagttccttc agtgatcgta
     1081 tcttttaaaa tgtctgtgat catataagct gtttctggag acatggctgt ttttccatct
     1141 ggctcataag tttcttctgt tccgtcttgg aagacgattt tattaacata ttgaggtttg
     1201 taataagtcc ctccattggc gaaagccgca taggctgctg ccatttttaa ggaagaagct
     1261 ccgtattttg tcccatcttg ttcttccgta ttactagaga tcgcattcga ttgatggatc
     1321 gtactgtatt cgatcccaag attttttaag aaagaagcca ctttatctga accgacttca
     1381 ttgaataatt tgactgctgg aacattacgg gataagtaaa gtgcttggcg taaagtaatg
     1441 gtgcccatat attgattatc ccagtttcct acaggagtcg aagtcccttc atagttatac
     1501 ggtgcgtcag taatagtttt tccagtagaa tatttcaaat attcaaaagc tggtccgtaa
     1561 tctgtgacag gtttcatcgt agaaccaaaa tcacgggaag tattcactgc taagttgttg
     1621 cctaaagtta cgtcttctgc gatgtgacgt ccgccaatct gggcttttac tttcccggta
     1681 ttggtgtcga taagggtgga ggcaacttgc atctcgtcgt ctgggtaaga gacatactga
     1741 tccgtgttga cgatatcata tagtttcttt tgggcgtcca gatccaaatt cgtataaatc
     1801 tctaaaccgt ctgtgtagac attcttatct gttttttctt gtacttcatt gatgacttct
     1861 ttgacataat tatcgacgat cttagtattg tcatcggatt gagttagttc ttgcagacca
     1921 tccgttaccg gtacattaac agcttgatca tattctgttt gagagatttt ttcgttttga
     1981 agcatcgtat acaaaacagt atctcgtctt ttcttcgcct gatctggata aacataagga
     2041 tcataggcgg aaggggcttg aggcatccca gcaagcaatg ctgtttgcgg taaagaaagt
     2101 tctgataatt tcttaccaaa gtacatctca gaagctgtct ccatcccgta caagccgttt
     2161 gacatataca ctttatttac gtagtaggtc aagatctctt gtttcgattt tttttgttcc
     2221 aatcttacag ccatccacgc ttcttgggcc ttgcgtttca gagtctgatc ctcggcactg
     2281 gtggagaaga aggaaagttt gatcaattgc tgtgtcaaag tacttccgcc ttgcagtccg
     2341 ccagaggtaa agttagagag ggcagaaccg atgatccgaa tagggtcaac cccgatgtgt
     2401 ttataaaaac ggcgatcttc taccgacacg atcgcatctt ctaatgtttt cggtaattca
     2461 ttcgcagaaa ttttttcacg cttttctgcg cctaaatctt cgaatagttc gccatcttgt
     2521 gtatatagtt tggaagatac ggtagcgtct aattttttat ccgtcaattc gggcgcatct
     2581 tttgcgtaat accagaaaag tcctacacct gctaaaaaga ggatacagaa aaatgagagt
     2641 attcctaaaa ggatcttgat aaataatcct tttttatgtg ttccagagga ctttccagaa
     2701 cctttgccag aattcttctt cacactcttt ttaggagtag gctgtttctg tcgtctagag
     2761 cttctagttt gttcatttgc cattcttgct ttctccttca atagataatg ttgataaata
     2821 gaagtcaacc gcgtccaaat aaggaatccg cggagaaata ccagttttga tctctattcc
     2881 tagttcttcg atcaaagata aaggtagtga tttgcgtcct ttttcctttt gggcgtccca
     2941 gtaagcaatc aaatgaacac tattcaaata aaaacagcgc ttcaaagacg aaaaccataa
     3001 caagacaaag cagaccccat tttgttcgag gcatgctttc atatgttcga tttgatgggc
     3061 atggaagttt ttaaagggaa aagatgtttt gttcttggtt tcttttgctt caaagtccaa
     3121 gtaaaagcct cgatagacgc cattatagtc tgtagttgag gcttgggtaa agtaggcttc
     3181 cttgatcaca gcagcacttc tcttaggata gtctactttc acgatttgga taggggtagg
     3241 ctttttatgg ataaccgcca acccgcggga aagataatat ttattacttt cattgattgc
     3301 ttcttcaaag cgcatccccc gattgccgaa ttcagtgatc tgatttcttt tttcttgaat
     3361 tttttcgacg ggagaggcat tcttataata aggttggcca tttggatagc gcaataccat
     3421 ctttaatcac actcctaggc cacattatag caaaaaagcg gatagaaaga aaaggaatct
     3481 gagatggata agtcaaaagt aatctatgtg tctgggtatc gaagtttcga actcggtgtt
     3541 tttaaagaaa atgatcctaa aatccaagtt gttaaaaatg ttttaaaaaa agaactccat
     3601 cagttagcag aagaagggct ggaatgggta cttgtcagtg ggaatttggg cgtcgaacta
     3661 tgggctgtgc aaatagtagc ggaattgaaa gaagaatatc cggaactatc tcttggtatt
     3721 ttgtatcctt tttctgattt tggtaataac tggaatgaag ggaaccaaga aaaaaaacaa
     3781 ttagcagaac aattagcaga ttatgtcgaa gctgttagcc atcaacccta ccaatcacct
     3841 agtcagctaa agaatcacac tcgttttata cttgagcata cagtgggctg tctgctgatc
     3901 tatgatgaag aattccctgg aaaaacgaag tatttttttg aagatgcaca gaattaccaa
     3961 gcagatcatc cctacgaaat tcggctgatc agtatggacg atcttcaaaa ttttagtgaa
     4021 taatcgatat tgtgtttgat ttatcaactt ttttcctata caatgaaaaa ggtagaaatg
     4081 ttattacgta aatgaaaact agaggtgtaa atatggcgaa tttggtttat agtcctaaag
     4141 acattttaca acaagaattc aaaacaaaaa tgcgcggcta tgatcccgtt gaagttgatg
     4201 aattcttaga caatatcatc aaagattatg aaacttacag caaagaactc cttgctttac
     4261 aagaagaaaa cgatcgattg agtgcaaaag tagctcaatt atctaaaact caaggagcag
     4321 ctcaaacacg cgtgcaacaa acagaggcac caaagagcgc agcagtgacc aattttgata
     4381 tcttaaaacg cttgtcaaat cttgaacgtg aagtatttgg taaaaagctt gatcaacaag
     4441 caagtgcagt caaaccagca caacctaatc caaataacta cacaaatgca gacacaagct
     4501 tagatgataa tgaaaaaaca cgtcaatttt aatttttgat taatgaattt gcggttttcg
     4561 ggtaatcgcg gtctgacttg atcaggctga ggaaagtcca tgctcgcaca ggctgtgatg
     4621 cctgtagtgt tcgtgcttag cgaaaccata agctaaggta ctctttggag taacggcagg
     4681 aaaacaagct aaggcttcgg ctatgcttaa gtatccttga aagtgccaca gtgacgaagc
     4741 ggctggggaa acccatccgg tggaacgcgg taaacccctc gagcgagcaa cccaaacaat
     4801 ggtaggggcg ctttttgtct ggaagaagaa caggacaaaa aggcagtata actgcagata
     4861 gatggttacc gctttgtttt ttctccctgg aaaagcaaag tacagaacat ggcttataga
     4921 aaaccgcaaa catcagggaa ttaggtcgtg acaaaagtaa tgcgttataa gaaacaagaa
     4981 aaacatttga aagctatttt tcaatacttc agatgaatcg ttcttattac aaaacaaact
     5041 tttgtatcac cggttattcg gaatttgagt aaaagtgtgg ggtaggactg atgtcccacc
     5101 tccttttttt ataaataagg agattgtgta tggaaaaaaa caggaagttc aacctcgtcg
     5161 caactgcggc aagcgggcta gaagcactgg ttggtaaaga attaagagat cttggaatcg
     5221 aatgccaagt tgagaacgga cgtgcacgtt ttgaaggaac aatggaaacg atcgctacag
     5281 ctaatttatg gttacgtaca gcagatcgag taaaaatcgt ggttggggaa ttcgatgcgt
     5341 atacgtttga tgaattgttt gaaaacgtaa aagcattgcc ttgggaagat tttcttccac
     5401 tagatgcagc ttttcctgta gctggaaaat cgatcaagtc gaaactatat agcacacctg
     5461 attgccaagc gatcacaaaa aaagcaatcg ttgaacgtct ccgtacctat tatcaccgtc
     5521 cagcttctgt accactgacg gaaactggcg cacattttca gttggaagta gctttgctga
     5581 aagaccatgt gatggtaact ctggatacga caggaccaag tttattcaaa cgtggttatc
     5641 gcttagaaaa aggtggagca ccattgaaag aaaacatggc agcagcactt attatgttga
     5701 caaattggcg aaaagaccgt ccattttatg atcctgtatg tggttcaggt acgctttgta
     5761 ttgaagcagc cttaataggc cataatatcg caccaggatt caatcgtgag tttgcttgcg
     5821 aatcttggga ttggttcagt caagaaatca tggaaaatgt tcgatcagca gccgaagaaa
     5881 aagcagatta tgatattgaa ttagatatta ctggttcaga tatcaatgga cggatgattg
     5941 aaattgccaa agccaatgca gaagaaatcg gattaggctc ttcgattacc tttaagcaac
     6001 aagcagtcaa agacttcaaa acggataaag aatacggggt catcgtggct aatccgcctt
     6061 acggagaacg tctaggagaa gaagaaactg ttcggaaatt atataaagaa atgggcgatg
     6121 tattccgtcc attaaaaaca tggagtaaat atatcttgac tagcgactta gcgtttgagg
     6181 aattttacgg acaaaaagca accaaaaaaa gaaaattgta caatggtgca ttgcgtacgg
     6241 atctattcca gtattgggga actcgtccgc ctagaaaaaa agagaaagac gaataatctt
     6301 actaattgat tcattcaatt ggatgtttgg aggaataggg aatgaaagaa aaagagtttt
     6361 tacaagaagt aaaagaaatc aatcttctac aacaagcttt aggaatcctc gattgggata
     6421 cacaaacagg tatgcctgaa aaagctagtg cataccgaag tgaagtagac agctatcttt
     6481 acagcattta tttttcaaaa aaaatcggtc caaaaatcaa agaagccatt cgctattttt
     6541 ctgaacatcc ggaagaattg tcggaagttg gaactgccgt ttttgccaaa gtaaaagaag
     6601 agtatgattt ggaacatcgc gtaccggaag atcttatgag aaaatataca gctgccgttt
     6661 cttcagcaca cggacaatgg caaaaagcta gagaaacaca acaattttct gattttcaac
     6721 acgcattgaa tcaaaatatt gaattaacaa aacagttgat accttactgg agaaaagatg
     6781 aaaagacgaa ttatgatgtt ttgctgaatc aatacgaacc cgggatgact gtcgaaatcc
     6841 ttgataaggt atttgatcag cttaaagaag gaattctttc gatccgccgt acgctccaag
     6901 aaaaagggac tgaacctgaa acagactttt taaatcggag aatgacgaaa aaacaacaga
     6961 agagatttgt agtgaaagta atcgaacaac ttggttatga ttttagtcgt ggtcgtttag
     7021 atgacactgt ccacccattt atgactggaa ttaatccgaa tgatgcacga atcacgacac
     7081 gatggaacga aacagatttc aaaatggctg tttttggagt gatccacgag gctggtcatg
     7141 gtatgtatga acaagacatc gatgataaat atgcgtatac accggtttat gaaggtacat
     7201 caatgggtat ccatgaatca caatcgttgt ttaacgagat cattattgga agtagtcgca
     7261 gcttctggaa aaagcaatat cctttctttc aagaatgtgc agaaggaaca ttcgatgata
     7321 ttccttttga caaattttat cgttcattga accatacaaa atcaagtttg gttcgtatcg
     7381 aggcagacag tttgacttac cctctccata tcatcattcg ttatgaaatc gaaaaaatga
     7441 ttttcaatga aggggtagat gttgctgaac tacctgaaat atggaatcaa aaatatgaag
     7501 agtatctggg aatacgacca acaaatgatg tggaaggcat cctacaagac gttcattggt
     7561 caggtgcaag ctttggttat tttccctctt atgcattagg gtacatgtat gcggcacaat
     7621 tacttcattc tttgcagcaa gaaatagatg tggaaaatgt tctagcgtca gacgattatt
     7681 ctccaatcaa aaactggatg tcagagcata tccatcaata tggtgcatct agaaaaccaa
     7741 atcaattaat catggatgca acacatgaac cgcttaatcc gcaatacctg atcgactata
     7801 tgaaagacct gtattttaat gtatatcagg taaaataaaa tacaatttac aaagatagct
     7861 gaaattcatc taatctatag atgttttcag ctatttactt aagaaagaag tggaacgaat
     7921 ggataaatta caagtgatcg ctgattatag tatggaccaa ttagcgcatg atcaaacagg
     7981 acatgggagc gatcatacca aaagagttgt caagttagct gaaaaaattt tggagacgga
     8041 accgcaggca gaccgctttg tgacgcttgc agcagcttac ctacatgata cgattgatga
     8101 taaagtcgtt aaaaatgaga acgaggcaaa gcaacagctt cgtgtattcc ttcgctcttt
     8161 gccaatagta gaggaacaga tctcgatgat tttttccatt atcgaaaata tgtccttttc
     8221 taaaaatctc tctgaagcgg ccgaactatc tctggaaggt aagatcgttc aagacgctga
     8281 tcgtatcgaa gcattaggag caatcggcat tttgcgtact gcttatttcg gcggtggaca
     8341 tgggcacccg atttttgatc ctgaactgta tccgcaaaca ttcaaggaca aagaaaatta
     8401 ccgaaaaggc acgacagtga tcaatcattt ttatgaaaaa ctgttcttgc tgtctgataa
     8461 aatgaatact gattacggat atgaagaagc caaacgaaga gaaaccttca tgcgtgaatt
     8521 cttagaagaa ttttttctgg aatggtctat tgacgatgaa aattttactc cagacaattg
     8581 gatcaaaaat aacaaaaaat gatggaggtt gtgacagaag tggtcagctc caagatattt
     8641 cggatcattt tccgtaaaag ttacttctgg aacactgttt accaagaggt cgtgagcctg
     8701 ccgttcagct ccgagtcaca aggaacaatt tcaaaaaata gcttctcgtg tttgatgaat
     8761 attgtaactt gtgattcgga attggcagga gaacaccgtt tatt
//
LOCUS       DAZBTF010000097         8740 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000097 DAZBTF010000000
VERSION     DAZBTF010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.098"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..219)
                     /locus_tag="ACHGNS_002562"
     CDS             complement(<1..219)
                     /locus_tag="ACHGNS_002562"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938455.1"
                     /translation="MSENKKKWLLIHPFDYISRTNILEEILTENSIEYFNVVSDYSHI
                     RKQFLSSKERHMIKNRHVIKTIGYKKNIS"
     gene            complement(212..841)
                     /locus_tag="ACHGNS_002563"
     CDS             complement(212..841)
                     /locus_tag="ACHGNS_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFN6938456.1"
                     /translation="MSNRGIYEKFFKRLIDILFSILGLIMLWPLMTFVAILVRIKLGS
                     PVIFKQQRPGKHEKIFLMYKFRTMTDQRDEEGNLLSDDIRLTQFGKILRSTSLDELPE
                     LWNILKGDMSIIGPRPLLVEYLSLYSNEQRKRHFVRPGLTGLAQVSGRNAISWKEKFE
                     KDIEYVENISMMKDIKIFFLTLKKVIVRDGITSVASGTTEKFEGAKIDE"
     gene            complement(834..1952)
                     /locus_tag="ACHGNS_002564"
     CDS             complement(834..1952)
                     /locus_tag="ACHGNS_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFN6938457.1"
                     /translation="MENKRILLASPHMSDEGYEQEYVKEAFDTNWVAPLGMNVNGFEE
                     ELAEYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPIIYQGAIPV
                     FVDSDYETWNMNPEHLRTALEKYPNAKAVLVVHLYGLSAELDEITKICKEHNVVLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERIEKKRYIHSFYKKELSKLSNIQF
                     MPENNWNEPNYWLSVITVKGINTNHLIDELDKNNIEARPIWKPMHLQPVFDKYEYIGT
                     DVAQTLFEKGICLPSDSKMTDDDLYRVTNTIKRVMQNV"
     gene            complement(1954..2586)
                     /locus_tag="ACHGNS_002565"
     CDS             complement(1954..2586)
                     /locus_tag="ACHGNS_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFN6938458.1"
                     /translation="MKAIIVGAGGFGKEIAFLLESFSRYELIGFVDDSLKMQNQELVG
                     KTVLGTIDSLLELEEETAIFLGIASPDIKEKIYQKINKNNKLIFPNLVASSALVGINV
                     QLGIGNILMPYTTYTADVVLGNFNMINIGSTIGHDTQIGNYNSIFPSVNISGHVLLGD
                     KNEIGVGTKIIQNLRIGNMNIIGAGSVVIKNINNKTKNVGVPTKVIERWD"
     gene            complement(2597..4414)
                     /locus_tag="ACHGNS_002566"
     CDS             complement(2597..4414)
                     /locus_tag="ACHGNS_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFN6938459.1"
                     /translation="MFVLTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFGSLSASAASSIIILLFINKQYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYLSTDSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVIGFVDDDPNKRNTYLSGKKVLGSLKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESAHVKVNTMPSMEELASGKISVSKLKTIDVVELLGRDEVELDIESI
                     KDQITNKVILVTGAGGSIGSEICRQIIQFDPAKLLLLGHGENSIYLIDRELRTHHQKC
                     PTEIVPIIADIQDRKKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPVTVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSMQTVMEFVKSLPQDDEQLAKDIVTFANASNK"
     gene            complement(4427..5191)
                     /locus_tag="ACHGNS_002567"
     CDS             complement(4427..5191)
                     /locus_tag="ACHGNS_002567"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFN6938460.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLQLGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVAQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(5219..5917)
                     /locus_tag="ACHGNS_002568"
     CDS             complement(5219..5917)
                     /locus_tag="ACHGNS_002568"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFN6938461.1"
                     /translation="MARTQKQKATQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSRDAGYYYGN"
     gene            complement(5929..6708)
                     /locus_tag="ACHGNS_002569"
     CDS             complement(5929..6708)
                     /locus_tag="ACHGNS_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFN6938462.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGSAPARPKATNDKTAPSRRSRSRV"
     gene            complement(6724..7668)
                     /locus_tag="ACHGNS_002570"
     CDS             complement(6724..7668)
                     /locus_tag="ACHGNS_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFN6938463.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTLQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(7714..8493)
                     /locus_tag="ACHGNS_002571"
     CDS             complement(7714..8493)
                     /locus_tag="ACHGNS_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938464.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKNFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
BASE COUNT         2368 a         1799 c         1473 g         3100 t
ORIGIN      
        1 ggatatattt tttttatatc cgatcgtttt tatcacatga cgatttttaa tcatatgcct
       61 ttctttactg gatagaaatt gttttcttat atgtgagtaa tcagatacaa cattaaaata
      121 ttcgatactg ttttccgtca gaatttcctc taatatattc gttcgactga tataatcaaa
      181 aggatgaata agcagccact tttttttatt ctcactcatc tatcttcgca ccctcaaatt
      241 tctcagtagt cccacttgct actgatgtta ttccatcacg gacaataact ttctttagcg
      301 ttaaaaaaaa tatttttatg tctttcatca ttgaaatgtt ctccacatat tcaatatctt
      361 tttcaaattt ttctttccaa ctaattgcat tacgaccact gacttgagct aatccggtta
      421 aacctgggcg cacaaaatgt ctttttcgtt gttcattact gtatagtgaa agatactcaa
      481 ctaacagagg acgcggtcca ataatactca tatctccttt taaaatattc cataattctg
      541 gtagttcatc aagactagtc gaacgcaata ttttaccaaa ttgagtcaat ctgatatcgt
      601 ctgataataa attaccttct tcatctcttt gatctgtcat tgttcgaaat ttatacatca
      661 gaaaaatctt ttcatgtttt ccaggtcgtt gttgtttaaa gatcactggc gatccaagct
      721 ttattcgaac taggattgca acaaacgtca tcagtggcca taacattatt aaacctaaaa
      781 ttgaaaaaag aatatctatc aaacgtttaa agaacttttc gtagatacct ctattagaca
      841 ttttgcatca ctcgcttaat tgtattagtt actcgataca aatcatcatc ggtcatttta
      901 gaatctgaag gtaaacagat acctttttca aacaatgtct gagcaacatc tgtaccaata
      961 tactcatact tatcgaaaac tggctgtaaa tgcattggtt tccatatcgg gcgggcttct
     1021 atattatttt tatctaactc atcaataaga tgattcgtat ttattccctt tactgttatg
     1081 acacttaacc aataatttgg ttcattccaa ttattttctg gcatgaattg tatatttgac
     1141 aatttagaca attctttttt ataaaaacta tgaatatatc gttttttctc aattctttca
     1201 tttaaaactt tcaattgtcc tcgaccgata cctgcaacga tattgctcat tcgataatta
     1261 tagccaattt cattgtgttg ataatggcgt gccttctcac gagattgcgt agcccaaaac
     1321 ctcacttttt ccaccttttc tttgtcatta gacacaagca ttccgccacc tgaagtcgtg
     1381 attatcttat ttccattaaa agaataaatt ccatagtcgc cgaatgtacc agtatatttt
     1441 cctttatatg ttgttcctaa tgattctgca gcatcttcaa ttaaaacaac attatgttct
     1501 ttgcatattt tagtaatttc atctagttca gctgataaac cgtacaaatg cactactaat
     1561 acagcttttg cgttaggata tttttctagc gctgttcgca aatgttcagg gttcatattc
     1621 caagtttcgt aatcactatc aacaaatact ggaatagctc cttgatagat gattggattg
     1681 gcagtagctg agaacgtcaa agactgacaa atcactatat catctttacc gaccccagcc
     1741 gcttttaaag ctagatgtat agctgctgtt ccagaagata gtgcagctgc atgactagca
     1801 ccgacatatt ctgctaattc ctcttcaaaa ccattgacat tcatacccaa aggagccacc
     1861 caatttgtat caaatgcttc ttttacatac tcttgttcat atccttcatc actcatatga
     1921 ggagatgcta ataatattcg tttattttcc aacttaatcc catctttcta tgactttggt
     1981 aggtacgcca acatttttag tcttattatt aatatttttt attactacac ttcccgcacc
     2041 tataatgttc atatttccaa ttcttagatt ttgtattatt ttagtaccta ctccaatttc
     2101 atttttatct cctaatagaa catgtcctga aatatttaca cttggaaaaa ttgaattata
     2161 attaccgatt tgagtatcat gtccgatagt agagccgata ttgatcatat tgaaatttcc
     2221 taaaactacg tcagcagtgt aagtcgtata gggcatcaaa atattgccaa ttcctaactg
     2281 aacatttatg cccactagtg cacttgatgc aactaaatta gggaaaatca atttattatt
     2341 tttatttatc ttttgatata ttttctcttt gatatctggc gaagcgattc ccagaaaaat
     2401 agcagtttct tcttctaatt ctaataacga gtctattgtt cccaatacag tttttcccac
     2461 taattcttga ttttgcattt ttaacgaatc atcgacaaaa ccaatcaatt catatctaga
     2521 gaaactttct agtagaaaag caatctcttt gccaaatcca ccagctccta caataattgc
     2581 tttcacttta ctccccctac ttattcgaag cattcgcaaa agtgacaatg tctttagcca
     2641 attgttcatc gtcttgcggc aggctcttga caaattccat cactgtttgc atggaataac
     2701 ctttgatgtt tcccacaaag atcttctcgt aaactgcttc atcgtttctt tccttatcta
     2761 acaatagttc ttcatatagt ttttcccctg gacggatacc tgtttcaatg atttcgatct
     2821 cgtcttcgct atatccgctc aatcggatca tgttttttgc tagatctacg attttcactg
     2881 gttcactcat atctaaaata aagatttcgc cgccttttgc taacgcccca gattgaataa
     2941 ctaaacgact tgcttcagga atcgtcatga aatatcgggt catacgaaag tctgttacag
     3001 tgactggccc tccttgagca atttgttccc ggaatactgg aatcacacta ccgcgagaac
     3061 caaggacatt cccaaatctg acagctgaga atttcgtaca tccttcttca ttcaacccag
     3121 tcacgatcat ttcagcgatt cgttttgttg aacccatgac gtttggcgga ttgttggctt
     3181 tatctgtcga gaccatgacg aagtttttta ctttcgctgc tttggctgct tctgctacat
     3241 tttttgtacc gaaaatattg tttttgactg cttcttttgg gttatattcc atcaatggta
     3301 catgcttatg agcggctgca tggtagacga tatctgggtg gtattgttcc atgatctcat
     3361 tgattttctt gcgatcttgg atatccgcaa tgatcggcac gatttctgta ggacactttt
     3421 gatgatgcgt gcgtaattca cggtcaatca agtaaatcga gttctcccca tgacctaaaa
     3481 gtaatagttt tgcagggtca aattggatga tttgccggca gatttctgaa ccgattgatc
     3541 ctcctgcacc ggtgacaaga atgactttgt ttgtgatctg gtctttgatg gattcgatat
     3601 ccaactctac ctcatctctt cccagtaatt ccactacgtc gatggttttt aattttgata
     3661 cgctgatttt ccctgaggca agttcttcca tagaaggcat cgtattgact ttcacatgag
     3721 cagattcgac taactcgaag attcttcgta atttttttct tgataaagaa gggatagcga
     3781 tcgttaccat ttggatgtca tatttttcga tcaattccgg tatatctttt aatgacccca
     3841 gtactttttt tcctgaaaga taggtatttc ttttattcgg atcatcatcc acgaatccaa
     3901 tgacatggat atcttgagcc gttttagaac caagaaagct gttgtataaa atacgtccac
     3961 cttcgccggc cccaacgatc aaggtatttt tagcggaatc tgttgataga tacctcatat
     4021 tttttgtttc gatatagata cgccagatca atcggctgcc aatgatcagt aatagcgaaa
     4081 gtaggtatgc aaagatcact aaacgtaaac tgtattgttt atttatgaaa agcaatatga
     4141 tgatacttga ggccgcactt gcagataacg aaccaaaaat cgcaatgatc tctcttaaat
     4201 ttgtataacg attgattcga gtaaaaactt tgaataaact accatagaat aaataaaaac
     4261 caatcgacaa accgatagag attaaaataa ggtccattgg tatagctaca aacggcttca
     4321 tgaatttagc tgctgcaata ttcgcagcaa tcaacaacaa gctgtccaca aataccaatg
     4381 tggcaatttt tcttcttctt gttaatacaa acaccctaca ttccccctaa aataaaccaa
     4441 acttcttctt ttttacctct tcatagactg gataactgat ctcatccccg ttgatcaggt
     4501 cctttgcgac ttgtttcatc tgtgctactt tttccttacc aaagtccttg gcgatttgct
     4561 cataacattc ttttaagtaa aaggtacgtt tagagacccc atgggcatcg gaagcagcca
     4621 tttgcacgag gttatgtttg accatcattt tggctgtttt ctggatgtct tttccaaata
     4681 caccgacata acttggtgcg gtgagttgag ccaagcatcc catatctagg aatggcagca
     4741 aatgatttgg gtcttctcga aaatgcgcgt tgcgttctgg atggacgatc actggtgtct
     4801 ttcctagttg taacaactca aaaaagagcc attctgcata taacggcact tccagtgtcg
     4861 gaaattcgat tagaagatat gtatcatcta aatctgtaaa taaaatttca tctcggtgga
     4921 tgtcttcaat cagtgttccg gtaatacgta cttcttgtcc ttccaataac gtcaaaggca
     4981 attgtctttt ctctaattct gcttgtaacg aagcgactag agaaatgact tgtgattttt
     5041 cattgctgta ttttccatta ttgtgatggg gtgtgcataa aatatgtgtg atcccttgtt
     5101 gaatcgcttt ttcagccatg gcgatactgg catctatgtt ttctgctccg tcatcgattc
     5161 ctggcaggat atggcaatgt agatcgatca tttctcttgt ctcctctctt tttatttctt
     5221 aattcccgta gtaatagcca gcatctctgc tgtgttctgc tccattgtat acaacaccaa
     5281 ggactcgcgc ttgtaccata tctaacagtt cttttgcttt agtcaatgat tcttttcgtg
     5341 acgtattttc ccgtacgacc aataaggtgc catccgtttt tgaagacatg atctgtgcat
     5401 ctgttacggc aacgactggt ggcatgtcga aaatcactac atcaaatagt tgtcttgctt
     5461 ctgctaaaat ctgatccatt cttggcgaac ttagtagttc tgatggattt ggtggtttag
     5521 gtccacttgg cagaatcgac aaattgtcga ctggtgttcg ttggatctca tctgctacac
     5581 ttcctgatga actaagaaca gtacttaatc cgcttgcgtt gttcaacttg aacgttttat
     5641 agatgacggg cttacgtaag tctgcatcga ctagcagtac ccgttgacca gatttggcaa
     5701 aaacaacggc aatgtttgct gcagtggtgg attttccttc tccaggacct gatgaagtca
     5761 cgacaatcgt tttgatttgc tgaccagctg cggaggcaaa ctggatattc gtccggatcg
     5821 tacgatattg ttcggcgatt ggtgaagatg ggtttgttaa tgtgatcaaa ctgactgcat
     5881 gagtttgagt tgctttttgt ttctgtgttc gtgccatttt tcttcctcct agactcttga
     5941 gcggcttctg cgtgatgggg ctgttttatc gtttgtagct tttggtcgtg caggagctga
     6001 tcctttttta cggataccag cgttcaattc ttttgctgac atttctggaa ctgttcctaa
     6061 gattgggaat cctaatgtat ccgtcacata tttttcatct ttcactgtcc gatcaagtaa
     6121 ttcgaataag aatgccagac ctacgcctac catcattcct aacactaagc caatcactag
     6181 attcaattta ttatttggcg aaacaggtgt cgtattggca actgcacctg aaataatcga
     6241 gattttatcg acattcatca cgtctttggc gttttcttgg aaaacttgtg aagttgtatt
     6301 agcgatctgt tgagcagtca ctgcattcgt gcttattgcc tggatcgaga acatttgtga
     6361 attttgtgac tgattgacgg agatcgcatc tttgatttgt ccagcagtca ttttcacatt
     6421 gtcttctgtt tctaaacgat ctttgacttc atttagtact aaatcactga cgatcatatc
     6481 tttatacgta ttgatcattt gcaggttcgt attgacatca ttgacatttg ctgtgtctga
     6541 ttgtggcagt gtcacgatca attgtgcttg tgaactgtat tttggtgtca agatgaaaaa
     6601 tgttacgatt cctgagatgg ccaagccagc aaacattgag atgatgattg ttaccaagtg
     6661 tttgcgtaat gtttcaaaaa tttctttcaa gctgattgtc tcttccattt tttctccact
     6721 ttcctattta ttctctagat tcatttgatc ttttaattct tttttcacac gatcaagttc
     6781 ttgatcgctg actttttgat aggagatatt atcttgcatg aagccttctc cttgcagctg
     6841 atcttgcttg atcgtaccaa atgcctcccg ataatctaaa gcgatttttt tcatttcatc
     6901 aaagctcatg tctgttttca tattcgactc tagagcattc aaaacggatt gatagttgct
     6961 gacaccgttt aaactcaata ctttttttgc tacaccttct acgatctttc tttgacgctc
     7021 ttgtctacca tagtcaccat ttggatcttc ataacgcata cgagaataag ataatgcctg
     7081 atccccgttc aatgtaattt taccgattgg aaagtcatag ccatcttgtg agaaagtgag
     7141 gtcattattg acttcgatac caccaactgc atcaacgagt tcctttagcc ctttcatatt
     7201 gatagagaca tagtgatcga ttgggatgtc tacatatttt tgtagggtat ccatcgccat
     7261 ggcagctccg ccgaatgcgt aagcgtggtt gatcttgtct tttgtgtcac gacctacgat
     7321 gtcgacatac gtatctcgag gaatgctgac gatcgttgtc tgtttgtctt caggactgac
     7381 tgtggcaacc atcatcgtat ctgatcttcc ttgatcgacc cgacctaaat ctccagtatc
     7441 aatgcccatc aatagaacag agaaagattc ttgtttgtcc agatcaactg gtttcgttcg
     7501 ttctccttgg gttcgttcca ctgattcgta agttttctca atagaatgtg agagatccag
     7561 atataatttt gctcctactc ctacaattgc taatactaat acgataagaa ctgctaaaat
     7621 aatcagaaat atttttttcc ctcttgattt tcttcttcgt cttgccatag tttcctccgt
     7681 ttgctaacct tgcttgaatc aacgttttaa ctgtcaataa cttatattgc tatagcccat
     7741 ttgctgagca acagtttgtg cttttgttgc gtatgctgcg cttatttttt cattccggat
     7801 ctgctctatt tgactgagcg ccgtcaaata ttgatcgtaa gtagcggtgg cagtcacttg
     7861 accatccttc aacatcgtat cgaataactg atctagttgc gtgacttgcg cgatctgagt
     7921 atcagcgaat cctagatatt gcataacaac cgttttccaa ggactatctt taaaaaagct
     7981 catattttca cgtacacgag cgacatctgc acttgtcacc ttttctttga tgacgacatc
     8041 gtctacagct tgctgccagt ttgatacatt tttagtaaat aaatcgttga cagctgtctg
     8101 gatcttcagt tttgattctg cactatctaa ctgtgtcacg attgctttct tctcatcttg
     8161 gattgcaaca gcttctttag gcaattctga ttctttgatg tcgtaatcat ctgctgaaag
     8221 tttgacagag gatagcttat ttgacaaact tgtgattgtt tcttctgtta tttcagggtt
     8281 tagaaaattt ttttcttttg tactatacag cttgtctact tcttttgata aagaagtcaa
     8341 ggtgtctttt tgattggtga tggcagattc tgcataattg atccgttgct gatgttgccg
     8401 ctgattattg atatggtaac caaacaaaca gatcgcaata gcacctgcac ctaccgccac
     8461 agcagtcaat gacttgacga atggcttttc catagattca ctcctaagta ttgatgatag
     8521 ataaattaag atataattca catacataaa aatgacagct ttttatttta aaaaacataa
     8581 ttaaattaat atattcacag aaatatttgt aaacaagttt tccctatata atcataccac
     8641 aaattgtttt cttgactagg gcaatttacg atttaaaagt aaataaaaca ttaataagat
     8701 aacaattaat cgatttcatt tttatttcct taattttcat
//
LOCUS       DAZBTF010000098         8701 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000098 DAZBTF010000000
VERSION     DAZBTF010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.099"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(39..635)
                     /locus_tag="ACHGNS_002572"
     CDS             complement(39..635)
                     /locus_tag="ACHGNS_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFN6938465.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            985..1329
                     /locus_tag="ACHGNS_002573"
     CDS             985..1329
                     /locus_tag="ACHGNS_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFN6938466.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            1330..1749
                     /locus_tag="ACHGNS_002574"
     CDS             1330..1749
                     /locus_tag="ACHGNS_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFN6938467.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1841..2191)
                     /locus_tag="ACHGNS_002575"
     CDS             complement(1841..2191)
                     /locus_tag="ACHGNS_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938468.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(2157..3488)
                     /locus_tag="ACHGNS_002576"
     CDS             complement(2157..3488)
                     /locus_tag="ACHGNS_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFN6938469.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            3743..4309
                     /locus_tag="ACHGNS_002577"
     CDS             3743..4309
                     /locus_tag="ACHGNS_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN6938470.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            4454..4541
                     /locus_tag="ACHGNS_002578"
     tRNA            4454..4541
                     /locus_tag="ACHGNS_002578"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4486..4488,aa:Ser,seq:cga)
     gene            4877..5851
                     /gene="bsh"
                     /locus_tag="ACHGNS_002579"
     CDS             4877..5851
                     /gene="bsh"
                     /locus_tag="ACHGNS_002579"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFN6938471.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
     gene            6176..8071
                     /gene="asnB"
                     /locus_tag="ACHGNS_002580"
     CDS             6176..8071
                     /gene="asnB"
                     /locus_tag="ACHGNS_002580"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFN6938472.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(8266..8700)
                     /locus_tag="ACHGNS_002581"
     CDS             complement(8266..8700)
                     /locus_tag="ACHGNS_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFN6938473.1"
                     /translation="MKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIAIDQA
                     KKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKSKGAK
                     KASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
BASE COUNT         3063 a         1475 c         1625 g         2538 t
ORIGIN      
        1 aacattgtca atcaatgact aattttacaa aataaatatt atccaagaag accacttaga
       61 tattgacctc cataaatcaa ggccatacta tataagaaaa tcgacagtgg tttagcaatc
      121 aaaatgatta gtgtatatcg tttcactgac atgtttgtca atccagccat caaacataac
      181 gcatcgtcag gagcaactgg aagaaaaata gctagagcaa acagcttctc aaaacggttt
      241 tgattatcca gccagccaat atatttgtta tatgtcttat cgctaatcat ggaaagtatg
      301 aatggctttc cataacgacg ccctagcaga aaaatgataa tcgaaccaat gcagattcca
      361 atatagttat agataaatcc agcataaggg ccaaatatca ggacaccagc tgcagtactg
      421 atccctccag gaatgattgg aataactact tgcaggattt ggatgaagac aaaaataatc
      481 ggtcctaaaa tgatggaatc acctactaat cctcttaacg cattcaaatc tttgaaaaca
      541 cctaaattaa taaaataaat cgtcagcgcg attgataggc ctaatccgat aatggatatg
      601 aaatttatca gttttcgaga ggcagcaata ttcattattc atcaactcct tagaggttct
      661 ttgttaacta tactttacta gaaaagcaac taactgcgcc attttttgat atgacagaaa
      721 acaaacaatt ttgtaagttc atcataagta taaaagattg atctcaaaaa aacatccaac
      781 taaagactta tttaataact tgtttttttt aaaataaaaa aagcgttagc aactatgctt
      841 aaaaagcata tcgactgaat aaactaagca ttggacaggc tgaaagacta catttaaaat
      901 gaaatgcgaa gaaacaatag aaaataaagt tgccttagaa ccatagttga atcacctaac
      961 agtcgtgaag ggagaataag agaaatggca gaaaaacaaa ccgcaggacg agatcaatta
     1021 ggagattttg caccaaaatt tgcagaatta aatgatgatg ttttatttgg acaagtatgg
     1081 tccagagaag aattatctgc tcatcagcgt agtatcatca ccatatctgc attaatttca
     1141 ggaggaaatt ttgaacaatt gccagctcat ctgaagattg gcaagcaaaa tggcattact
     1201 agtgaagaaa tagcagaaat cattacccat ttagcttttt atgtagaatg gccaaaagct
     1261 tggtcagctt ttagtttagc aaaagaaatc tatttagaag aaaatagcga agataagaaa
     1321 gaggcgtaaa tgatgacaaa acacgaagaa atacacaatg gcgtgatttt tccagcaggt
     1381 gaaaaaaacg aagcttacgc tcaagtattt accgggcaaa gctatctgaa tatgttagtg
     1441 aatgatcccg aagtttctgt tggtgtaggt aatgtaactt ttgaaccagg ttgtcgcaat
     1501 cactggcatg tccatacaga cggctatcaa atcttacttg tgaccggagg agaaggctgg
     1561 tatcaggaag aaggaaagcc agcaagacga ttaatggctg gagatgtcgt cgtaacgcac
     1621 aaagggatta aacattggca tggtgcgaca aaagacagtt ggtttgaaca tttggcgatc
     1681 actgctggta attcagagtt tttagaacct gtaacagatg aagtgtatga aggattggaa
     1741 aatcaataat gtcaagcttg aacagctcct gaaagtagat tttaatcgaa ctttcaggag
     1801 ctgctttttt gattcagtct tttataaata gaaaataggg ttaataatag gaatgagaag
     1861 cacgtcgaaa gatcttaata ttgataatcg gctgggcata catgatttcc acgtcatcaa
     1921 tcaaactacc taacagatga taatcttccc gatcatgtcg gggattacct aatagttctt
     1981 gatagtagat ttccatacta gggtcccgag gattgttcag caaatcagca agtacatcta
     2041 aatcttctgg ctgagtttct gccgtaccct gtatctctat gaaaagccct tcctcagcaa
     2101 attctactcc aatacgtatt tcagttattt catgggaata taaataagta agaagctcat
     2161 cgatgattct ttttgataat tctttttcca agtgtctttt tttcctttct gacagcggta
     2221 aagttatcta gtatcggtac aaggatacct gcaacgaaac cagttgaaaa accactgtta
     2281 tatagattta gtcctccgtg caaagtgctg acgttatgga caagagcaat atgtagcaca
     2341 ccagctatta caccgtaaaa actgccgtaa aaaccgctta tgggcgcaag acctgtccca
     2401 aacacagctg caacaagcat gccagtactg gtgatctcat gaaaagttcc aaacaaagaa
     2461 gcaataaata ttccgactag tatcggaaaa ctgtttttta aatgacaacc aaaagcactg
     2521 aaacccaccg cagataaaat agcaccgatg acaggtccat ttaactgacc gccaaccagc
     2581 aaaacataag aagacagcat cagaccaact aagcccatgt tgatcaaagt agcaccaata
     2641 ccagaattag caatgaaatc agttgtcaat tttccagatg aatcgaaaat ttctcgaatc
     2701 cccgaaagcc gaaaggaatt ataatagaaa ccaattccga ctgtcagcga aaaaaagcca
     2761 aaaataatcc aaatgagagg aaagtgatat tcattgaaaa ccaatgtatt ttcgacgatt
     2821 gaatagctga ataacctcaa aacagcagta aaagtcatcg caatcaaccc agaggtgaat
     2881 ccaatattat aaagtgtgaa tccacgatgg aaaactaaag tttgagcagc taagggcgga
     2941 agaagtatac caattgctat gccagctaaa ttccccaata aaatacctat cagtaaagga
     3001 aaacgcatac caaaagtgat ataactaacg accggtgata atacgctacc aaacagtccg
     3061 atcatgatgt actgagaaaa aggtttattg acaaatacag cgtatagata cacacctaaa
     3121 atgattgaga tagaattata aaggtttttg ccaaaaaaag agaaaccggt aactgttaga
     3181 atcgaagcaa ataagggacc gtttaaaatc accttgtgcc gataagctaa aagcacagaa
     3241 agtaaggtca aaatccctac atttatgaat gcgcttccga aaacacctaa agcaaaataa
     3301 tctgtcaaaa gattgcttgg cgaggtaaga atagttagca atccactcca attatcagta
     3361 ggagtattag aaagcaagcc aataaaaaga aaaaaaacac ctgtaaaacc aagataaaaa
     3421 tactttcttt tctgtgaaac gattcgtttt tgaggatgaa tataaataac gtcagtttcg
     3481 ttttccatta cacaccttct ttttcatttt ctttaactat aacttgaaat gaaagatttg
     3541 gacaactatc atttaggtca aaactaaaaa tgtaagggaa cccattgaat ttaaccaaat
     3601 ttttttaatt ttgcaaaaaa aagttaaaga agactgaaaa aaatttaaaa tataacgttt
     3661 tgagtaaaat gtttgcaaaa aatgctaaaa atagggtaca ataaaacaac atatactgca
     3721 gtaaagaagt gggggagttt taatgagaag aaaagtctat accaaggatc aaattctaaa
     3781 agctgcttat gatgttgttg caaaggaagg gtttaaagga tttactgcga gaaatattgc
     3841 taaaaagatg gggatttcga cacagccgat ttatctggaa ttcaaaaaca tggaagattt
     3901 aaaaaatacg ttgctggatt ctatctttga acaactaaaa gaagatgttt tccctattga
     3961 acgtactggt gataaaatca ttgatctcgg tttgaactat atctactttg caaaaaagca
     4021 tcatagtctt tacatggcat tgtacattga tgaacatggg gacggaaaga aaatgcatga
     4081 tttttcatac aattatttcc gtgaaatagt taaagaagat gaaagataca atcaattatc
     4141 ggatgaatat gtcgatgctc tgcatactgg tacatgggta gcagtgactg gcttagcgtc
     4201 attgatgtca tcaaacatca tgcatccaac agaagaacaa atcattgcac tgatcaaacg
     4261 gacaattgac tcgattttag cattagataa tcctgaaata atcagatagt gttctatcag
     4321 tatgaatttg atcggtaagt ccgttggctg caaactgtca atggactttt ttgctaaaaa
     4381 aagcgatttc tgttatttat ctattagaaa taggcttgac atttcgtgaa aaaagtttaa
     4441 aaatagtact tacggaaggt tggcagagtg gtaatgcacc ggactcgaaa tccggcgaac
     4501 cggctaatac cggcgcgcag gttcaaatcc tgtaccttcc ttatatcaac gattagaggt
     4561 tgtgaagaag ctgtcctgca tcaagtaata agaacggcca aacaaaaata gcttttccta
     4621 ttttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc gtttgttcgg
     4681 aataaggggc tgtgacaaga cttttgtcac agccccttat tgttgtactt ttaacctaca
     4741 atattttttg attccccctg agtactattt tttataaatg ctagtaaatc aatcttcttt
     4801 ggcctttttt ttgataaaaa aaaatactag atgtaagata aaagtgtaaa aacgatttgt
     4861 tttaggaggg aaaacgatgt gtaccgcaat cacttatgca acgaaagacc attattttgg
     4921 aaggaatttt gattatgaga tgtcttataa tgaagtagtc actattactc caagaaatta
     4981 tcgtttcgat tttagaaagg taaaaaattt agataaacat tatgcaatga taggaatagc
     5041 agctggggta tccaactatc cactctatta tgaggctaca aatgaaaaag ggttgagtat
     5101 ggcaggactg aattttcctg gcaatgctga ttataaagaa ttacaagaag gaaaagataa
     5161 cgttgcacca tttgagttta ttccatggat actagggcaa tgctcaacca tagacgaagc
     5221 taaagaatta ttggctacta tcaatctagt caatatcgat tttagtgaaa aactaccctt
     5281 gtcaccttta cactggttat tagctgataa agaaaaatcg atagtaattg aaagtatgaa
     5341 agatggactt catttatatg ataatcctgt cggtgtgttg acaaataatc cgccatttga
     5401 ttatcaatta tttaatctga acaattatcg ctcgctatca aatggaacac cagaaaatca
     5461 tttttcaaac cagatcagct tgaatgttta tagtcgtggg atgggaggat tgggcttacc
     5521 tggagatctc tcttctgttt ctcgatttgt aaaagccaca tttactaaaa tgaatgctgc
     5581 atcaggtgat tcagaatctg aaagtatcag tcaatttttt catatactag gctcagtcga
     5641 gcaacaaaaa ggagtatgcg atactggaga aggaaaatac gaatatacga tttattcttc
     5701 ttgctgtaac gttgataaag ggatttatta ttatcgtacg tatgaagaca gtcaaattac
     5761 tgctgtaaat ataaataaag aagacttgga tcgtcatgaa ttggtcagtt atccagtcat
     5821 aaaagagcaa cagataaact atgtcaatta attaatgaat agatccgttt ggtatcatat
     5881 tttctgatac agtcataatt agacacgatc tttaggtcgt gtcttttttt gctgtttttt
     5941 ttggaaatag catccactaa tattttttta acacgaagag aataaggtag cctcaaaaaa
     6001 gaaaacatgg taaattagat attgggactt tattttctgt aaaaacagaa actttcatct
     6061 tttgttttca cgtgacgaaa ttgtaagaga gaaaatattc gtccgctatt tattctatga
     6121 taagatgcat atgaatgaaa taaacagtaa ttttatatta ggatgtgaaa gttaaatgtg
     6181 cggtattgta ggttttgtta acgacaagga caacaaaaaa acaattatta atacaatgat
     6241 ggatcgaatc gttcaccgtg gacctaatag ttcaggagaa tatatcgata aacatgttgc
     6301 tttaggattc agaagattaa gtattattga cttagagggc ggtactcagc ctatttacaa
     6361 tgaagatcgg acaaagatca ttatttttaa tggcgaaatc tataattacc agcctttgag
     6421 agaagaattg attgcagctg gccacgtttt tcaaacacat gcagatacag aagtcctttt
     6481 gcatggttac gaagaatggg gaacagaatt attgcaaaag atccgcggaa tgtttgcttt
     6541 tgctatttgg gataatgaaa agaatgagtt attcggtgca agagaccact ttgggatcaa
     6601 accatattat tatgcagaaa tgaacggaac atttatgttt ggttcagaaa tcaaaagttt
     6661 tctgcctcat cctgatttta acaaagaatt gaacaaagaa gcgttgaaac cttatatgac
     6721 tttccaatat tctccattaa acggcgaaac attcttcaaa gatgtctatc gtttgcctga
     6781 aggccattat tatacgtata aagatggaaa actagacatt cagcaatatt gggatgcaga
     6841 ttttgaaaca aaagaaacgc attcccgaca agaatggatc gaaaaaatcg acgaaaccgt
     6901 tcaagcttct attgaagcac atacggtcag tgatgtagaa gttggttctt tcttatccag
     6961 cggggttgat tccagttatg taacctctgt attgaaacca gatcattctt tctcaatcgg
     7021 gtttgatgat aaaacctata atgaagcgat tgaagccaga aaactaaccg aactattgga
     7081 cttagataat acagcagcag tgatcgacgg agacatgtcg ttcaaagcat tccctttgat
     7141 ccagtatcac ttagatgaac ctgattcaaa tccttcttgc gtacctttgt actttttagc
     7201 aaatcttgct tcacaaagtg tgcgggtagt ccaatctgga gaaggggcgg atgaactgtt
     7261 tgcgggatac caaacgtatg gattccatac gaattcaaaa ttcatccgtg tgattgctca
     7321 aggattgaaa aaattaccaa aaggaacacg ctataattta ggacgtaaaa tcggaaaaat
     7381 gaaaaatttc catggacgga tccatttata tgaatcactt gctcctgcaa aagaatattt
     7441 tatcggtcat gctcgagtgt tcgaagaatc agaagcatca gaagtgttaa caccaaaata
     7501 tcaaacagca ccatctgtgg atgaaatcat gactgttcat tacgagaaaa cagaaggaat
     7561 caaagatgaa gtcaataaga tgcaatatgt cgatcttcat caatggatgc caaaagatat
     7621 cttgttaaag gcagataaac tttctatggc aagttctttg gaagtccgag tacctttatt
     7681 ggatatcgaa gtcatgaaat tggctcaaca aataccaagt aagtttttat tgaatcaaaa
     7741 caacacaaaa gatattttca gacaagctgc gaacaaacat cttccggaag aatggtcgaa
     7801 tcgtgtgaaa ttaggattcc ctgtgccaat caaagcttgg ttgaaagaag aacacggcta
     7861 cgaacaagtc aaagcattat tcgaagcaga ttttgcaaaa gaattttttg atcaagaaaa
     7921 aatcatgaag ttgctagatg accatcacga aggacgtaaa gaagagcaaa gaaaaatctg
     7981 gacaatcttc agtttcttga catggtataa agtctacttt gtagatgaaa cgattccgca
     8041 agcagaagca atcgactatg tgactgtttg atgaaaatca aatatcaata aaaaagaggt
     8101 tttctcctac cgtttaacac tgagtgataa gaaacaattt caaaaaacag ctcttcccgt
     8161 tttatagata ttatgactta tggttcggag ttggcagagg aacaccattg tttcgaacat
     8221 aagggggttg tgacacgatt ttcgtcacaa cccctttaca tatgttcaat aatcgatttc
     8281 tttttctttt gtcccatctt ttttataaat gaacaaagtt gattctttgt tttgtgctac
     8341 ttgctttcct ttttcgattg cttctttttt tgtatcaaaa tggttgcttg ccttttttgc
     8401 ccctttagat ttcacgatcc attgttcttc ctcatattct acgatcacat ctgaatcaag
     8461 cagttttcca gcacgaggat tcgtatcgtg ttcatccgtc ttgcttggat ttttttcttt
     8521 ttcgaaagtt cgtttttctg attcactagc atcttcatac catttttttg cttgatcaat
     8581 ggcaattggg atcgcacggt tatcagggta cccttcatct aataatgcat ttgcgatgtc
     8641 gattgctttt tttcttgtca atttatctaa gtttttcatt gatgcaggaa agtccttcat
     8701 g
//
LOCUS       DAZBTF010000099         8593 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000099 DAZBTF010000000
VERSION     DAZBTF010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.100"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(132..1085)
                     /gene="menA"
                     /locus_tag="ACHGNS_002582"
     CDS             complement(132..1085)
                     /gene="menA"
                     /locus_tag="ACHGNS_002582"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFN6938474.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1098..2201)
                     /locus_tag="ACHGNS_002583"
     CDS             complement(1098..2201)
                     /locus_tag="ACHGNS_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFN6938475.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2291..3226)
                     /gene="pplA"
                     /locus_tag="ACHGNS_002584"
     CDS             complement(2291..3226)
                     /gene="pplA"
                     /locus_tag="ACHGNS_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFN6938476.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3528..5462
                     /locus_tag="ACHGNS_002585"
     CDS             3528..5462
                     /locus_tag="ACHGNS_002585"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFN6938477.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5919..6335
                     /locus_tag="ACHGNS_002586"
     CDS             5919..6335
                     /locus_tag="ACHGNS_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFN6938478.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6349..6915
                     /locus_tag="ACHGNS_002587"
     CDS             6349..6915
                     /locus_tag="ACHGNS_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFN6938479.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6938..7924
                     /locus_tag="ACHGNS_002588"
     CDS             6938..7924
                     /locus_tag="ACHGNS_002588"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFN6938480.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8079..>8593)
                     /gene="scm"
                     /locus_tag="ACHGNS_002589"
     CDS             complement(8079..>8593)
                     /gene="scm"
                     /locus_tag="ACHGNS_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFN6938481.1"
                     /translation="SDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSDSTDT
                     TSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHA
                     SVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRI
                     TFMSYKKSKR"
BASE COUNT         2524 a         1656 c         1749 g         2664 t
ORIGIN      
        1 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
       61 aaggagctat ttttatttgg ctgttcttat tacttgatgc agggcagttt tttcacaacc
      121 tcttcatgct gttaaatggc ttgcataatg attgttagta atagccccaa tacataactg
      181 gcattgaata agaccatatt tttgatggaa tagctgaaac tgataggctg gggtaacgtt
      241 tgcttgaatt gatccaaatg cttcttcaca attggcaagg tcaagaaggt gaccaagatc
      301 ggccattcat agataccgct caacaacccc attaaaatca cgaaatagca agcatacatc
      361 aatagctgga ataagactgt tcccgcagga cgtccgatat aaaaaactag tgtatagcga
      421 tgattttcaa tatcacggtc aagatcccgc aaattattag ccaacatgat attggcaatc
      481 gtaaacacca agggtaagga agctaatacg attgctgcta tagcccaaag atttcctacc
      541 aaagcaaatt gtccagatga aaagttgact gtcagatcat agacatctga accgaatgta
      601 ttgacataaa cgaccatggc aaaaattcca aggcccatag tcaccccact gaaaatttcc
      661 cctaaaggca ttcgagataa aggaatcggt ccgaatgtat agaatactcc aataaaacaa
      721 cagataaatc ccatcaaaag taaaagccaa cccgtattga tcgttagaat aacgccgata
      781 acaaaaacaa atgcaatcat agctaaaatc atgtttcgaa tcaatacagg agatagattt
      841 ctgcggccaa tgacattttc ttcatactta tatacttcag aatgagcctt ttcataatcc
      901 atgtaattat tgatagcggt agtcgctaaa tcaaaaacta gcattccaat aaaaaataaa
      961 aaagtgttta gccaatgtat ctcatgaaaa aaagcgatag agaataatac ccctaccgca
     1021 aaaggaaata cactagccac ctttgttcgt atttcaacta cttctaaaaa gatttttagt
     1081 gtcatggttc gtactcctta ttttagttca gaacggtcac ccatcgtgta atgggagtcc
     1141 tccgataatt tgaaggtgtc tttgattgga tcggtcacat atactttatc gtcttttgtc
     1201 acgaagatcg cttccgtccc gttattcaat tcgctttcga cgtattccag tccttttttc
     1261 acacccattg aaaagactgc tgtagacaac ccgtcaccat caattgattt atctgttatg
     1321 atcgtgacac ttgcgatatc attgtcgaac ggataacccg tttttggatc aaacaaatga
     1381 tggtattttt tgccatctac ttctaaataa cgctcgtaaa ttccagaggt aactaatgtt
     1441 ttgttccgtt ctttaataga acctaaaaca gatccgcgag ccatgttcgg atcttggatg
     1501 ccgactgtcc agtcttggtt ttcgcctctt gggctgtggc ctaagacata gacattaccg
     1561 cccaagtcaa cgatggcagt ggtcactcct tgttttttca agactttcac tacttcgtcc
     1621 gtaatgtaac ctttggcgat tgcacctaaa tcgatgatca ttcctttttc ttttagataa
     1681 acagtttttt ctttgtcatt tagttcgatc ttatgataat ccactaattt caatgcctga
     1741 tcgatttctt cttgggaagg ttttctggcg tcatcaaaac cgattcgcca taattgtgtg
     1801 attgcaccaa tcgccatatc gaatccgcct tgagaatctt ggctatattc gtaggcccgc
     1861 ttgactaacg tgtagacgtc atcagaaact tttactggtt tgatcccagc ttgctcattc
     1921 acttcatcga tctcagaacc tttttgattg atcgtgattt tatcaccaag ttcttttact
     1981 cgatcgaagg caggtttcaa tgctgattct ttgccttcat catacacacg gatacgaacg
     2041 taagtcccta acaagaactg ttcatcagaa tatggatctt tcaatatctt ggtagctgct
     2101 gaactttcag tggttgtttt atttgctgag ttgctgcagc cagttagcac cataaaagag
     2161 atgcccagtg tgattaagga aaatagtagt tttttccgca tctttttcct ctcttcattt
     2221 tgaaaggtga gacgactgcc taatctggct atcgtcccac cctccaactt ttattagtat
     2281 gattatgtga ttatttttct gttacgatat tatctacttc gattgtttga gtgtcgcctt
     2341 tttcagctgc attcactaat tgttgtgcgt acatgatgaa tgaatgtgag ctgtgtgtag
     2401 cacctgtaac aacttctaca ttagcaggtg agccatcttc ttcgcccatt gctttgacga
     2461 attcgtcgtt cagagtaggg atgtaagttt ttggttctgt accgcttttt gctttcatat
     2521 tttcattata ttctgtatca tcttgttttg atttaccgtc tttgtcgata taatcaaagt
     2581 ttgattcagt tactttgccg ccagcaactg tcatttcgaa ttgcacacgg tagccgttag
     2641 agtagttctt ttcttcaagc ttgtaagtgc cgtctttcaa atcagcacca ttgtcgattt
     2701 cgattgtatc tgtgttacca gcttgagcag cttggatcaa ttgttgtgcg tagttttgga
     2761 agctttcaga tgagtgagta gctccagtta cgacttccac accgcttgcg ctttgtgctt
     2821 ttaagaattg gtcattcaac tcaggaatgt atttttctgg gtttgtgcca gattttgctt
     2881 ccatgttttt gttgtattct gcatcttttg tttttgattc gccgttttca ttgacgttat
     2941 catattttga ttcagtgatt ttgccgtctt ttacaaccat ttcaaagact gcacggtaac
     3001 catttgaata atttttttct tctaatttat atgtgccgtc ttgcaaatct ccgccagcta
     3061 caacttttgc agatgattct gttgtgcttt ttgcagtcga agattgtgct gtagatgatt
     3121 cagaagaaga gtttgagctt ccgttgtcat ctgcgccgca agcgcctaac actagagctg
     3181 aaaatgctaa tacggcaaat ccagttacaa aacgtttttt cttcatttta ataccccacc
     3241 attccatttc tagtccacaa tttttctttg tgattcatta tacattctaa tctttttagc
     3301 ctataagtca actatttttg ataaataacc catgtaatat ttcaaacgtt ttctttcagc
     3361 aaatttcgtg aaaaaacgct ttattttaaa atttgaaaaa aatccaaaag gagttagaca
     3421 aaagaacaga acatctatat aatatatgga gattgtgtaa taagtatcaa actaaaagaa
     3481 ttatcatata ttcattatat agatagtcta taaggagcgg atgatcaatg gcaaagcaaa
     3541 atattgtcgt tgtaggagca ggatatgccg gtgtttcagc tacaaaatac ttggcaaaga
     3601 aactgaaaaa agaagatgta gcaattactc tgatcgatcg tcattcgtac catactatga
     3661 tgaccgagtt gcatgaagta gctggcggac gtgtagaacc agaagcaatc caatacgatc
     3721 tgcagcattt gtttgctcgt caaaagaatg tacaacttgt cacagacact gtaatcggta
     3781 tcgataaaga acgaaaaatc gtcaagacca aattaggttc ctatcctttt gatcagctga
     3841 tccttggaat gggcggagag ccgaatgact ttggtacacc tggtgtcaaa gcacacggat
     3901 tcaccctttg gtcatttgaa gattctgtaa ggattcggga acatattgag aagacagtag
     3961 caaaagctgc attggaacca gatgatgaac tgcgtaaagc catgctgact ttcgttgtct
     4021 gtggttctgg atttaccggt attgaaatga ttggggaact gatcgactgg aaaaatcgcc
     4081 tagccaaaga ttataaatta gatccaaacg aaatcacact gatggtcgta gaggcaatgc
     4141 cgacgatctt gaatatgttg gatcgaaatg atgctgcaaa agctgaacgc tatttgcgta
     4201 agcacaacgt ggaattatta ttgaatgcac caatcgtaga agtggctgct gatcatatca
     4261 aattgaaaga cggacgcaac attccgactc acacactgat ttggacagct ggtgtcaaag
     4321 gaacttctga cgctgctgac tttggattag aatcagcacg cggacagcgg ttgatcgcca
     4381 atgaatatat gcaggcaaaa ggctacgaag ataaaaacat ctatatcatt ggtgatttag
     4441 tcttttatga agaattccca ggaacaccga caccgcagat cgttcaagct gctgaacaaa
     4501 caggacacac agccgcagcg aatatcgtgg cttcgatcaa aggcggagaa aagcataaat
     4561 ttaaaggaaa ctatcaagga tttatggttt ctattggtgc caaatggggg gtagctaacc
     4621 tatttgataa gatccattta agcggatttt tagctatcgt aatgaaacat atcgtgaatt
     4681 tgaaatactt ctttgatatt cgttctggtt attatatgtt ccaatattta atgcatgagt
     4741 ttttccatat caaagatgat cgcaacgtga ctcgaggaca ttcttctcgt tacggcaatg
     4801 tcttatggag tgtgccactt cgtattttct atggtttagt ttggttcgtt gaagcttcca
     4861 aaaaaatcat tgggaatggt gaagtgttca aaccaagtac ttggtttggt gaaggatcat
     4921 ggttcactgg aaacagtgtg ttcccttgga catgggaata caatgtaaca gatgttgcca
     4981 gtggtgcttc tgctgctggt gatgcaacca cagctgccag cggagcaggg gcaagtgccg
     5041 gagaagcagc gactcaagct gcacattttg gtttgagtta tgcatacggt gaagaaccaa
     5101 tggctgtgat tgagaagtca ccagactggt tcacaaagat catgcaagtg atcatgccaa
     5161 acaatgatgt agcaatgttc ttccaaaaat tcatggtatt ctttgaaatt gccttggcat
     5221 tagcattgat tgctggatta ttcacatggc tatgcagtgc gacaacgatt gtattggtga
     5281 tcacgttctg cttatcaggc atgttttact gggtaaacat ttggttcatt tttgttgctt
     5341 tcgcattgat gaacggttcc ggacgtgcga ttgggttgga tcgctgggta attccatggc
     5401 tacaacgtac actaggtaat tggtggtacg gtagaccaag ttcccagtac ggcaagaaat
     5461 aaatagataa agaacgttat ttgaatgcaa gatttcaata gaaaggggct gagacaaacc
     5521 gtcacagcct cctttttatg taaaattaaa ggaataagat gcccgagata agcctccatg
     5581 agcaagacac aggtgtcaca acctctgtta caacctcttg ttataaacgg tgttccagaa
     5641 gtagcttctt cgaaaaataa gccgaaattt cacaaaaact tgaaaagtaa ttttcgtaaa
     5701 ttccttctta tttcttgaag ttgcccactt ctgtcacaac ctcttgttat aaacggtgtt
     5761 ccacctgctg cttctcggta ataagcccaa agaattagaa aacatggaaa gctgttttct
     5821 attcttcgtt cttattactc aaagcaagac acaggtgtca caacctcttg tttttatgct
     5881 aaaattgggt gagttaatct ggaaaaagga gaacaaaaat gagccttaaa gaatttgctg
     5941 aaaaaagtct gctcaaacct tgggatgttt ttattatttt gcttttgact gttctttctt
     6001 ttttaccagt catgattttt ggtcttcagc aaaacaccgc aacagcagaa aaagaagcgg
     6061 tactgcgtgt ggatggcaca gaaatcaaaa cattcccttt gatggctgga gaagagagca
     6121 acacatatac ttacaaagat gaacacggag attataactt gatcgagata gatggcgatc
     6181 gagtacggat aaaagaagca gattgcgatg accagatctg cgtacgtcga ggctgggcaa
     6241 cgaaaaatgg cgaaaccatc gtctgtttgc cacataaatt agtgatcgaa gtccgttcaa
     6301 cagatggagg tgacgaagac agtctgattt attagacctt cacacaccat gagtaaatta
     6361 caaaaaatga tttatatttc gctacttgtt gcacaaggag tgatcattgg tttattggaa
     6421 aatatgatcc cttatccttt ttcttttgcg cctggtgcaa aactaggctt agccaatcta
     6481 atcacgatcg ttgccatttt taccatgaaa aaacgagaca gcttcttact actttggcta
     6541 cgactattcc tgactacttt gctaggcgga acgatttcca cattccttta tagtatgagt
     6601 ggtgctttgc ttagttattt tggaatgtta ttggtcaagc aattaggacc aaaacgtgtc
     6661 agtatcatcg gaattagtgc ggccggcggt ttcatgcata atgtcggaca gcttttgatg
     6721 gcttctttca ttgcacaatc atggacagtc atgttgtact tgccagtcct ttcagtttta
     6781 gggattttat cagggattgc aatcgggatc gcagcgaatt atcttttacg acatgtgcag
     6841 acacttcaac gattccaaaa agattatgag cagggaacca aacaaaaatg gagcccaatg
     6901 tttaccaaaa aataatcgtc acacaaggag tttttttatg aagctacatc caatgtggga
     6961 aaactatcct gctttaagta aagaattgac agaaacacta aaacggatgg aagaaactgt
     7021 ccgcctgaaa aacaaacctg tcgaacaagc aatcaaagaa acgatccatg ccggcggaaa
     7081 gctgttgcgt cctgcctatc agctattgtt ctcacaattt ggaccagagc aagatcgaga
     7141 caaagccatc gctttggcag cttctatcga aatgctccat acggccacac tgatccatga
     7201 tgatatcgtt gatgaagcgg atctgcgacg tggtcagcct aatatccgta gtcagtttgg
     7261 taatgtcgtt tctgtctatg caggagacta tttatttgtc tgctgtttta aactattgtc
     7321 tgattactcc acttccttga aaagtctcca attgaattct cgcagtatgg aaaaagtatt
     7381 gaatggcgaa ttagggcaga tggatgatcg ctataattat gaactttctg taaaagaata
     7441 tttggaaaat atttcaggta agacagctga actgttccaa ttaagttgtt ctgttggcgc
     7501 ttttgaaagt ggaacaagcg agcgcttcgc aaaaaaagca ggagatatcg gactttctat
     7561 cgggatggcc tttcagatta tcgatgatat tttagattat acaaaagaga gccaggaaat
     7621 cggcaaacct gtactagaag acatgcgtca aggggtctat tctttaccgt tgatctactc
     7681 tttgcaaaaa aacaaaccaa agctactacc ttatttagag aagaaagcgg caatgacaga
     7741 agaagatgtc gacgccgtac gcaaaatcgt tgaacataca ggaggcgtgg aagaagctag
     7801 gaagttagcg gcctcttaca cagaaaaggc tttgaaggaa ataaagaaat tacctgccac
     7861 ctctcttcga acaaaagaga atctcttttc ccttacccaa ctgattttgg atcgcaaaga
     7921 ctgaataggg aaaggttcga tacgtaaata aatttctcag aaggaaatct acgtgttttt
     7981 tgacaaattg aattgtagtt cttgtattta tgataaaaag caaaaataaa gagcaacgtc
     8041 cgttagcgac gttgctcttt tttatcttga atattttttt accttttcga tttcttataa
     8101 ctcataaaag tgattcttaa accgattaca agtaatataa cgcctgcgag tgttatccaa
     8161 ttattcgatt gacttccagt tttaggaagt gctctttttg atttgtttcc attgtcattg
     8221 cttgttttat tagttaagtt aaagttcgtc ctgttatccg aacttgtttt tgagaaggag
     8281 acacttgcat gacgtgtatc gcttgtggta gacgacgtgt ctgtactatc gcttgaagtt
     8341 gatgaatctg agctatcact tgtggtagag gatgtatctg tactgtcact tgaagctgac
     8401 gtgtccgtgc tatcacttgt agtggtagat gaatctgagc tatcacttgt ggtagaggta
     8461 gtatctgtac tgtcacttga agctgacgtg tccgtgctat cacttgttgt ggtagatgta
     8521 tccgtactgt cgcttgaagc tgacgtgtcc gtgctatcac ttgtggtaga ggtagtatct
     8581 gtactgtcac ttg
//
LOCUS       DAZBTF010000100         8335 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000100 DAZBTF010000000
VERSION     DAZBTF010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.102"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            476..1339
                     /locus_tag="ACHGNS_002590"
     CDS             476..1339
                     /locus_tag="ACHGNS_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFN6938482.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ACHGNS_002591"
     CDS             complement(1468..3090)
                     /locus_tag="ACHGNS_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFN6938483.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ACHGNS_002592"
     CDS             3339..3860
                     /locus_tag="ACHGNS_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFN6938484.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACHGNS_002593"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACHGNS_002593"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFN6938485.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ACHGNS_002594"
     CDS             5941..6759
                     /locus_tag="ACHGNS_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFN6938486.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ACHGNS_002595"
     CDS             complement(6862..7059)
                     /locus_tag="ACHGNS_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938487.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ACHGNS_002596"
     CDS             7228..7806
                     /locus_tag="ACHGNS_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFN6938488.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="ACHGNS_002597"
     CDS             7901..8038
                     /locus_tag="ACHGNS_002597"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938489.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2516 a         1490 c         1642 g         2687 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagtata aaacggtgtg gtttttggaa taaaaaaata acata
//
LOCUS       DAZBTF010000101         8229 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000101 DAZBTF010000000
VERSION     DAZBTF010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.103"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            328..1251
                     /gene="truB"
                     /locus_tag="ACHGNS_002598"
     CDS             328..1251
                     /gene="truB"
                     /locus_tag="ACHGNS_002598"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFN6938490.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1255..2196
                     /gene="ribF"
                     /locus_tag="ACHGNS_002599"
     CDS             1255..2196
                     /gene="ribF"
                     /locus_tag="ACHGNS_002599"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFN6938491.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2591..3376
                     /locus_tag="ACHGNS_002600"
     CDS             2591..3376
                     /locus_tag="ACHGNS_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN6938492.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3389..4240
                     /locus_tag="ACHGNS_002601"
     CDS             3389..4240
                     /locus_tag="ACHGNS_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFN6938493.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4364..4816)
                     /locus_tag="ACHGNS_002602"
     CDS             complement(4364..4816)
                     /locus_tag="ACHGNS_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6938494.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5281..5649)
                     /locus_tag="ACHGNS_002603"
     CDS             complement(5281..5649)
                     /locus_tag="ACHGNS_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFN6938495.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            5799..6281
                     /locus_tag="ACHGNS_002604"
     CDS             5799..6281
                     /locus_tag="ACHGNS_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFN6938496.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            6318..7328
                     /locus_tag="ACHGNS_002605"
     CDS             6318..7328
                     /locus_tag="ACHGNS_002605"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFN6938497.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(7443..8156)
                     /locus_tag="ACHGNS_002606"
     CDS             complement(7443..8156)
                     /locus_tag="ACHGNS_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938498.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         2784 a         1337 c         1667 g         2441 t
ORIGIN      
        1 ttaagaaact gaaaaaataa ataaaaaaaa acggaaaaaa gactggaaga atggacagtc
       61 ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc
      121 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaaaacataa
      181 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
      241 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
      301 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat taactttatg gaaagaacgc
      361 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
      421 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
      481 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
      541 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
      601 gatatgtctt tatctgaaga aaaaatagat gaagtgatgg cttcatttat tggtgagatc
      661 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
      721 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
      781 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
      841 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
      901 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaagat
      961 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
     1021 ctgccaatag aaaaaggagt gaaacgattc aaacgagtag atattactga tgaagtatgg
     1081 cagaaagtaa aaaacggtat gagacttaat tatactgtat ttgcgctccc agaaatgccg
     1141 tcagaagaaa ttgctttgtt ttataaaggc aaagttgtca gtatctatca gccaaacccg
     1201 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
     1261 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
     1321 gtactaggat tttttgacgg cgtgcatatt ggacaccaga aagtcataaa gacaggtatg
     1381 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatcg
     1441 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
     1501 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
     1561 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
     1621 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
     1681 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
     1741 ggtgaaaaaa tcagctctac ccgtattcga ggattactag acagcggaca agtaaaagaa
     1801 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
     1861 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac agttcatttg
     1921 cctaaagaag gtgtttatgt atcggaaata aaaattgggg aaatttggta tccagccatg
     1981 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agaattgtat
     2041 attttggact ttaatcaaga tatctatggt gaacatgtcg cagtacgctg gaatcaattt
     2101 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaagaggat
     2161 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
     2221 ttatgtataa ctagcaattt caataaaatg ggtgataaat aagcccagga aaggggctgt
     2281 gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa gtagcttctt
     2341 cggaaataag ccgaaatttc acaaaaattt gagaagtaat ttttgtaaat tacttcttat
     2401 ttcttgaagc tggccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag
     2461 caattcatga aaacgattgc taaagcgtat catttgcttt agactatata tcttggtgta
     2521 cactcacttt aagacggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg
     2581 agtgggtact atgtataagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa
     2641 ggggattgga acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa
     2701 ctataactcg gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg
     2761 aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga
     2821 tacggcttta gaaaattttg ggacactgga cgtttggatt aataatgctg ggatggaaaa
     2881 tcagcatcca acccatgaac ttagccttga agattgggaa agagtattaa aagtcaattt
     2941 gactggcgtt tttatcggaa cgaaagcggc tttaaattac tttacagaga atgataaaaa
     3001 aggaaatatc atcaatatgt cttctgttca tgaacaaatc ccatggccaa cgtttgctca
     3061 ttacgctgct tcaaaaggcg gagtaaaact attcaccgaa acggtagcaa tggagtacgc
     3121 accaaaggga atcagaatca atagtatagg acctggtgct atccgtacac ctattaatgc
     3181 ggcaaaattt gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg
     3241 cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag
     3301 ttatgttaca ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca
     3361 aggtggaaaa ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt
     3421 tgggtggggg atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat
     3481 attaggaact ggtattggag ccgtgctcgt tggcttactt gttcaattat tcgcttctcc
     3541 agctggtatt tcagtgacta catttttgat tagtttactt tctggtgcat tctgggtcat
     3601 cggacaaaca ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat
     3661 ttcgactgca ttacagttag tcggaacctc attgattggt gtcttcgctt ttggagaatg
     3721 gtctggaagt atgggaaaaa tcattggtgc agcagcaatc attcttcttg tatttggttc
     3781 tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaatcag
     3841 tctattagca tcaacaagta ttggttattg ggtttacagt gcattgccca aactagttga
     3901 tgcaagcgga ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat
     3961 ttatgtgatt ttccgaaaac caaccgcatt ttctgaagac aagagttgga aagcaacaat
     4021 agttggagcc atcttcagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg
     4081 ggttgctact gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat
     4141 gtacatcctg catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt
     4201 gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaatct
     4261 catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa aaatagcgct
     4321 agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat acaagtgaaa
     4381 tatctgtctg catcaataat ttttcattga tccattgact aaaatttacg acatgattga
     4441 agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat tgtaaaagac
     4501 ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct cggatttgat
     4561 tgattgctgt aaaatcacga tactgaaggt tgaaacgcat cgtgattggt gcatttagca
     4621 tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg aactcaaaaa
     4681 gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt tttccttcat
     4741 gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat tcatttactg
     4801 agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact tagttgatat
     4861 ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta aaaatcaagt
     4921 cagaaaattc taacttttct ttctctaaag aaattcgttc tctcctaaag tgatattata
     4981 tttaaatata cctgaaaata atgggcagta ttatgaaaga tcactgatat tttctcgtat
     5041 tttttcctta ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt actaagtgga
     5101 aaattcaaag ccattcaatc agtgattttt ttcagcacaa gaaagactcc ttataaaaat
     5161 agcagatact ttgtcaatta agtgtttaac cagggatgat aaaaaggata ggattgaaca
     5221 aaacctgtat aagctgatga atagtttcct cgccagtaaa aggtttattc gtataaagct
     5281 ttatcgccaa aatttatcat gacctttttt atcttcaata ttaaatgaaa tcaattgttc
     5341 aatcaattcc cagttgatta cacttttcca aggaactttg atgacgttat ccgtatatac
     5401 gtagcctgct tcttggatat ttttttcaag ggcacgaatc gctgcttttt ccggtgcgat
     5461 agaaaaatga tttttagcaa aactaaatgc aataataaag gtaccatggt cagtaaacat
     5521 aggttggttc cattttacag ttgtgtctag ctggggatac gtttctgtta tcttagcaaa
     5581 aaggtctttg atcttttgtt gatgttcttt ttctttgatc tgattgatga tcggctgaaa
     5641 tttttccatt ctaaaacgcc tccattaagt ggtttgattt attttaccag aaaaacaata
     5701 gttatgtata aaaaacgaac aaaacacaag taatgttgta accgctatat ttatatgata
     5761 agatgtctca aataataaaa tagaaaagag acataaaaat gattgacttg gcaaagaaat
     5821 tcttacacga aaatatatat tttgagttgt atcatcatcg agccatttat acaaatgaag
     5881 atgctttggt aataaagaaa gaacaaggat ttacaggtac agagaccaaa agcttattct
     5941 taaaagataa aaaagataat aactacgtat atctgacttt tactacgaaa caaacggatt
     6001 tcaaagcatt agggaagatc gtcggtaaaa ggctttctat tgtttcatca gaacgcatgg
     6061 aagaacaaac gggacaaaag cagggcgctg tttctccgtt tggttatgaa aaacagattc
     6121 cgattattat tgatgaagag ctcttatcaa atgaaaaact tgtttttgca ccaggtagac
     6181 cagatcaaac gatggtcatc caaacggttg acttaaacaa aatcatccag ctacttgaga
     6241 tgccgatatt tttgatgccg gcaaacgaaa aaaacaatta accaagtaaa atagtgcata
     6301 cttggaggtg aagcaacatg ataagttatc cattattaga aagaaatgca acgattgggg
     6361 ttactgcacc atcctcaggc gtatcagagg aacaccattc gttactcgaa tcagctttaa
     6421 agagaatgga agaaaaatct ttccaagtag ttactggaaa gacgctttgg acgcaagata
     6481 aggaaaaatc aggtccagca agaaaacgag cagatgaatt caatcaaatg atgcaagatg
     6541 aaaatatcca aatgatcttt ccgccttggg gaggagaatt attgattgag attctcgatt
     6601 ttgtcgattt tgatgctcta caaccaaaat ggatagtagg ttattcagat attagtacgt
     6661 tgctacttgc tgtcacatta aaaacaggga tagccactgc acatggaacc aaccttattg
     6721 atttaagagg agagtacttg gatgaaacga cagcaatgtg ggaaagtgta ctgaaaacaa
     6781 gagaaggaga atcgattcta cagcaacctt ctgaaaagta tcaaaaagag tggccccatg
     6841 agcattcatc atcttctgtt ttccatctca ctgaaccaac aaattggcaa tcagtcagcg
     6901 gagatgcagt aaggatgaag ggacgattat taggtggctg tattgatacg atcagtcatt
     6961 taatcggtac cccttatgga gatgtccaat cttttaaaag aaagtataat gatggaaagc
     7021 cgatcttgtg gtttctagaa aattgtgagc tatcgtcagt tgatttaaga agatcacttg
     7081 ttcagatgaa actggcagga tggtttgatg gcagctcggg gatcatgttc ggtagaagtg
     7141 cagcagagga tttgttagat tataaaatga aggatgtata tgaagatctg tcaaaggaat
     7201 taaaactacc gatcttgtat gatattgatt gtggtcatgt accgccacaa ctgacattga
     7261 tcaatggtgc ctatgcagaa gtagcggtag aagatagaaa agcgactgtt ttgcaaacgt
     7321 ttatttaaaa aattttttcc tgccttggag tttatcaaag accaacaaat ctaaatagaa
     7381 aagaaaatga aagaagctga cagtattttg ccagcttctt tttgtatgat ctattagaaa
     7441 ttttaattat ccataactat cacattgatc agctgtttca catcatttgg attacctgta
     7501 ggaatagcta ctacagatgc attatcaata atttcttgag ttgtttctct aatgaacgct
     7561 ggataagatg tttgatctgc aggcatttgc caatgtgaag gaactggata actagtgatt
     7621 gtcccgtcat ggttacttga atacactaaa actccctggt aactgtattt cccagtgagc
     7681 ataaccacat ctttcggata gactgcactc cctgcatcat aaggggcgat aggtgttcct
     7741 gcaggataat gaataacatg tagttcgaaa tcacccgatg tatctctaaa agtattacct
     7801 ctaattgcta gccaaacacg tgcgtactca atttgtagat cagaatatcc tgctaacgga
     7861 tctattgact gagaagactg tgaggaagtt gtgtctattg ttgaagaaga aatatttgtc
     7921 tcaactgtac tactagatgt agaagaagta ttgggctctg agactttact gcttgttgaa
     7981 gatgtattcg tttcagtaga agctgatttc tgagtacttg atttttcagc tgaactggtt
     8041 tgtttgattg aacttttgtc tgtggtcgca gatggatcac tattttcttt actaggagac
     8101 gtacatccta caaggaataa agagaccaac aataaaataa aacctaactt tttcatataa
     8161 aatacctcat tattttttta ataccagtat agcataatcc tgaatttttg aatccgtaaa
     8221 accttgtct
//
LOCUS       DAZBTF010000102         8116 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000102 DAZBTF010000000
VERSION     DAZBTF010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.104"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            386..574
                     /locus_tag="ACHGNS_002607"
     CDS             386..574
                     /locus_tag="ACHGNS_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFN6938499.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            652..1665
                     /locus_tag="ACHGNS_002608"
     CDS             652..1665
                     /locus_tag="ACHGNS_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN6938500.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1732..2766)
                     /locus_tag="ACHGNS_002609"
     CDS             complement(1732..2766)
                     /locus_tag="ACHGNS_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN6938501.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            2881..5106
                     /locus_tag="ACHGNS_002610"
     CDS             2881..5106
                     /locus_tag="ACHGNS_002610"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFN6938502.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            5124..7007
                     /locus_tag="ACHGNS_002611"
     CDS             5124..7007
                     /locus_tag="ACHGNS_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFN6938503.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            6988..7962
                     /locus_tag="ACHGNS_002612"
     CDS             6988..7962
                     /locus_tag="ACHGNS_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFN6938504.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         2623 a         1477 c         1710 g         2306 t
ORIGIN      
        1 tttagttagt ctacattatt ttggcgattt gccaataagg actgttgagt tgtgcaaaat
       61 caaatctggg tcagaatgaa cgtcattctg tctcagattt ttcgttattt caatttgatt
      121 aactgattga tcaaaaagat ttttattttc atattctcaa atgattcaac actaaatata
      181 catgtttgac gcggaaatta ttattcgatt ttggcggcat caggggggat gacgacgtag
      241 cagacccatt attttactca agaaaatgtc cgtgcctatt atttagaata cgatagtgaa
      301 cgtgctggga gttttgttct tttagagaaa gtctctgaag ataaattagt tgtcttagat
      361 tttgactgta acgaaaaatg gcgaaatgga agagcaccag caagtgattg accggatcta
      421 cgaagcgaca cgatatctgc ctttggatcg tttgtgctta agtcctcaat gtggatttgc
      481 ttcaacagaa gaagaaaatg ttttgtttga agaacaatgg aaaaaatttc ttttgtcaaa
      541 agcattgcag aagaagtttg gggaaaatca ttaaatcatc taggagagct tgataggaaa
      601 taacactatg gtataatcag aatgcttaat actaactact gagggagcct tatgaaaaag
      661 aaaattctta aatttatcgg atggttcttc ggaatcattc ttgggatcgc gtttgcaatc
      721 tttcttgcct tccagctttc ccccagacct ggagcattct tgatcaacca tatgttttca
      781 aatactgttc aaatcactga taaaaaaacg ttcgatcaag cagaaaaaaa cgtggcgaaa
      841 aaaacagatt tgacgtatga gtccaagtat aaagagaata cttatgacat ctactatcca
      901 aaaaacagca aggggcctgt tcctgttcta ttttgggtac atggtggagg attcgtcggc
      961 ggagataaat cgggtgtcaa agaatttgcg acaaaattag tttcagacgc caacatcgca
     1021 gtggttgcga tgaattatga attagcgcct gattcagaat accctaatca agttcttcaa
     1081 gtgaatgaac tgatcaaaga tttattagct gaagaaaaag acgacaaatt gctagacatg
     1141 gaacaattat tttttggtgg agatagtgca ggttcacaaa tcgcattgca atatgcagct
     1201 atacaaacga atgatgcgta tgctaaacaa atgaaaatca aacagctact tccaaaagat
     1261 agtctgaaag gaaccatttc ttattgcgga cctgttgatt tgaagctaac agcacagcag
     1321 cattcggatg atcgatttat gaagtttttc gtcaaaaccg ttgcgtggtc gttgattgga
     1381 acaaaagatt ggaaaacgag tgagcaattg aaagaagcta gtgtggctga ccatgtttct
     1441 aaggaatttc cgccaacgta tataactgac ggaaatgcat actcattcca ggagcaaggt
     1501 caagcattag aaaatcggtt gacagaatta aaggtacccg tgcaatcctt gttttataca
     1561 gatacaaaaa aagagattac ccatgaatac caatttgatt acacgctaaa agaatcgcaa
     1621 aattgttacc agcagacatt ggattttgtg aataagtata agtaagaggt tgtgaaaaag
     1681 ccgtttcgtt ttgagtatga agaaacgaag cggcttttca caacctctct ttcaatacct
     1741 ttttgttctt gggataaaac tttccctaac aatcaatcgt gcaggataag tcaaaatgat
     1801 tggttcgtcc cgtataccgt caattcgctc caatgccatt cgaacagctg cttgcccaat
     1861 tgatttcgca ttgatttgaa cactggtcaa gccaggggag aggtttgcga ttgtctcgat
     1921 attatcaaag cttgctacaa caatctcttc tccaatttgg atattttttt gtttcaatgc
     1981 ccgatagaca ccaattgcca ttggatcgct cccgacaaga atggcagtag gaagagggga
     2041 ttcattttct aatagattag tcgcaagtac ctcaccttgt ttttctgtcc agccacctag
     2101 atgataagac atatattttt ctaagtgatg atcgatcata aaatgctgat aggcacgcat
     2161 cctcttttca tttatagaaa tctgcttccc accgaattca tccatatcga ctttatatcc
     2221 cccgatatag gctattcttt tatgaccatt ttctaagaac aattccagta cggttttagt
     2281 catccgttca agatctgcat acaccatatc gacctcttgc tggatgtcag ggttatccac
     2341 gacgactatt tgcatgtttt gttttaacaa ggcggtaatc gattctttcg taacagtccc
     2401 aacaacgatc accgcaccta actgatctag atcttcccat tgtttaggat tatcagatag
     2461 attatagatt cgattcatcc ctaaatgcag acgtcgagct tcttcttcca tccctactcg
     2521 caaatgtttg taatagatat cctgttcttc gctcgctttg ccgatcgtag agatcacacc
     2581 aatcgaaggc attctctttt tgatatattt acgagtactg atgtcataat ccaacgcagc
     2641 agctgcctct actatccgtt gccgagtagt ctgagccaca tggatcgtct gatcgttatt
     2701 caacacacgg gaaactgtag caggagagac atttgccttt cgtgcgacat ctctaattcc
     2761 aaccattttt acacactcct caaaaaaata aaaaattttt attgacaaaa ttgtttagta
     2821 aacagtatac aggaattata aaaaaaagta aacggtttcc gttttgttgg gagtgaaatg
     2881 atggatatta cgcatagaaa aacagataaa aatttcatct tatttgatga aaagagccga
     2941 gtcttccatt tacgaaatac atttctttct tatctgataa agatagaaga aagcaatgta
     3001 ttggcgcata tttattttgg caaaccagtc aagcagtaca aagacaataa aaattatccc
     3061 cgaagagatc gtggattttc aggaaatgtt cccttaaatc ctgaccggtc tctgtcgaaa
     3121 gatacactac cgcaagaata ctcttctcat ggcagcatgg actttcgaac gcccgcatcg
     3181 atcattcagc gcaagaatgg atcagactta ctggatctga gatatgacag ccattatatt
     3241 acagatggga agccagagat cgaaggcttg cctcaaactt atgtgatgga caagtcagag
     3301 gcacaaacgt tggtgatttc acttaaggat agagaaacag cgatttattt cgatttgttc
     3361 tataccatat ttactgatag agcagtgatc acacgatctg taaagataag aaatgaaacc
     3421 ggagaaacaa tcaaactaga aaaagcggct agtttccagc tagactttgc ccatactagg
     3481 cgatttgatg aagtcattgc tttgccagga gcacatgtca atgaaagaca gatttcacgg
     3541 caatctgttt tatctggaac aaaagtattt gaaagccgtc gaggaacgag cagtcaccat
     3601 atgaataact ttattgcttt agttcatcat catacgacag aaaatactgg agaagcgatc
     3661 ggacttcaat ttgtttattc cggcaaccat tctttcgaac ttgaaaaaga tcagatcaat
     3721 caattgagag tcgtcggtgg gatcaacagc catcgttttt cgtgggaatt gaatgcaggc
     3781 cagtcttttc aaacacctga aatgatcctt tcttactcaa gtcagggatt gaacaaaatg
     3841 agtcagatcc accatgagtt gctacgtgaa agaatcgcaa gaggtcgtca tcaatttgct
     3901 gaacggccga ttctggtcaa taactgggaa gccacttact ttgacttcaa tagtgaaaaa
     3961 atcaaggcga tcattgatga agcaaaggag ctaggcatcg aaatgttcgt attagatgat
     4021 ggctggtttg gaaaaagaga tgcggacaat tcttcattag gagactggtt cgagtatgaa
     4081 ggcaagttga caaatggatt acgtgaaata gcagattatg ctcatagcaa aggattgaaa
     4141 tttggtttat ggtttgaacc tgaaatgatc tcggttgatt ctgaactgta tcgaacacat
     4201 cctgattttc ttatgcagga gccaggtaga atgccttctg cttccagatc acaacatgta
     4261 ctggatttta caaggttaga tgttcggcag acgattgaga aacaaatgag aaagatttta
     4321 gatacgattc ctcttgacta catcaaatgg gatatgaacc gttcgctttc agatgtctat
     4381 tcgattaccc ttgacccaca gagacaagga gaagtagcgc atcgttatat gctcgggctt
     4441 tatgaactac ttgaacattt gacaactgat tatccggaaa tcttatggga aggctgttct
     4501 ggtggaggtg gacggtttga tgccgggttt atttattata tgccgcagtc atggacaagt
     4561 gataacacgg atgcagtgga gcgtatgaaa atccaatatg gaacaagcct tgcttatccg
     4621 atttcgtcca tcacagctca tgtatcagct gtaccaaatc atcagaccga cagatctact
     4681 tcgttgaaaa caagaggaga aacagctatg agcgctgttt ttggttatga actggatttg
     4741 acaaagctgt ctcctgaaga aaaaaagcaa gtaaaagacc aaattatatc ctatcaaacg
     4801 attcgtccgg tgatccagta tggccattat tatcgtttgg cgagtccttt tgaagaaaat
     4861 attgccgcat ggatgtttgt gtctcctaaa caagatgaag ctattgtgtt tttagggagg
     4921 atactagctt cagctcaacc agcgttccat gaagtatatc tgatggggtt agatgatgag
     4981 gcactttatc aggaacagac ctcgaagcgg atattttcgg gggccgaatt gatgacagtt
     5041 ggactttact tccccgattt tcaaggtgat ttccaaacag aactgcttca tttcaaaaag
     5101 ttatgagaga gaaggaaaaa agtatgaaag caaatataaa atggttagat gatccacaag
     5161 tttttagagt gaaccaatca aatgcacata gcgatcatgt ttgttatgca acaagggaag
     5221 aagtagaaac cacatcctcc cttaaacagt ctttaaatgg atcatggcgt ttttgttatt
     5281 cgccaaatcc ttcggtacga ccaaaaacat tttataaaaa agattttgat gcctgttctt
     5341 ttgatgaaat cactgttcct agtcatattg aattagcaaa ttatgacaag atccattaca
     5401 tcaatacgat gtatccgtgg gagggacatg tatttcgccg tccccctttc acgacaagcc
     5461 ctgagaaaga ggcggaaggg atcttttcag ctgctaaaga caatccggtt ggttcgtatt
     5521 tgacgacttt tcgattagaa gaaggtctaa agggaaaaca aatctcgatt cattttgctg
     5581 gtgtagaaca agcaatgttt atttggttga atggtcattt tatcgggtat gccgaagatt
     5641 cgttcactcc gtctgaattt gatttaacac cttatttgga agaagaaaat attctagctg
     5701 tggaggttta caaaagaagt acagctgcat tcttggaaga tcaggacttt ttccgatttt
     5761 ttgggatctt cagagatgtc ttcttagttg ctcgaggaca aaaccattta gaagatcttt
     5821 ggatcaaacc tcgcttagat gacaaatgtc aaaatggcaa acttgaagta gatttgtctt
     5881 ttgagcaagt agccaaaaac agtcagctga atgtagcgat actagatcct gatgggaagg
     5941 aaatctatca acagcaatat ccgttaaaag agcaaatgac gattcgcatc gatggatttg
     6001 aaaatgtggc actgtggggg cattttcagc ccaatttata taagcttgaa ttggagacga
     6061 tagacgaaga aaaaagagta acggagtata ttgactattc cttcggtttt cgtaaaatcg
     6121 aaatcaaaga taaaatcatc tacttcaatg gaaaacggat gattatcaac ggggtaaatc
     6181 gacatgagtg gcatccgaaa aaaggacgcg ccatcactat agaagatatg gaaaaagatt
     6241 tggctatcat aaaaaatgca aagatcaacg gcgtacggac atctcattac cccaaccaga
     6301 tcacttggta ttatttatgc gaccaagcag gaatccatgt gatggcggaa acgaacctcg
     6361 aatcccatgg ctcatggcaa aaaatgggcg catttgagcc ttcttacaac gtaccaggtg
     6421 cagtacctca gtggcaggcg gctgttcttg atcgagcaaa aacgaatttc gaaacattaa
     6481 aaaaccatcc aagtattctt ttttggtctc ttggaaatga atcttatgcg gaagaaaaca
     6541 ttcgacaaat gaacgattac ttcaaaaaag tagaccccga tcgacttgtc cattatgaag
     6601 ggaattttcc aaatccagca ttcgaagatt cgatttcaga tgtaaaaagc tgcatgtatg
     6661 cgccaccaga agagatcatt gactaccttg aaaataaagc ggaaaaaccg ttcatcttat
     6721 gtgaatatat gcatgacatg gggaattcgt taggtggaat ggattcttac atgacgttgc
     6781 ttgatcgtta tgaaatgtac caaggaggct atatatggga ttttaaagat caggcgatcc
     6841 aagtgactga tccaatcaca ggcagagaag tgatgcgcta tggcggtgac tttgacgata
     6901 aaccaagtga ctatgaattc tcagggaatg ggatcgtttt tgcagatgga caagaaaaac
     6961 ccgccatgca ggaggtaaga tattattatg aaaaatacag taaataaaag tcatatggat
     7021 acggaaaaag ttgcaatcgt cttcggcgac tgtacattag gtgtcaaatc ggggaatacg
     7081 cattatattt tttcttatac aagaggcgga ctggaatcgc tcaataaaaa cggaaaagaa
     7141 tggctatacc gggaaacact tccagttttt tggcgagcaa cgaccgataa tgacagaggt
     7201 agcggatttg gatttaaatc cggtatgtgg ttaggagccg gattgtatcc aaaagtcaaa
     7261 gaaatagaag tactggtgga caataaaaag atcgatttgc cgattgctcc agtaaataat
     7321 caatattcaa gtaatgaata cgctgaaacg gttgaaatca tttttacact ggaaatcaac
     7381 acagtaccaa aaacccaagt gacaatttcc taccaaatcg ctggagaggg taaaatgctt
     7441 gtcaatatgc attattatgg acaaaaagga ttaccggaat tacccgcatt gggtttaagg
     7501 ttcattatgc caacgaaagc aaaatttttt gagtatgaag gcctatcagg ggaaacatat
     7561 ccggatcgga tggctggtgg tgtcgaaggt atttataaag tggcaggact ccccgtttcc
     7621 ccataccttg ttccccaaga atgcggcatg catatggaaa cgaaatgggt agaactaaca
     7681 agaaatcaaa cgttggataa tacggataaa gatcaagaag atttttcgtt gcgatttgaa
     7741 cagcaggata cgccatttgc tttttccgct ttaccattca aagcagaaga actggaacag
     7801 gcaacacata tagaagaact accgccagaa agacgaacgg ttcttacgat ttttgcaaaa
     7861 gtaagaggag tcgggggtat caatagctgg ggagcggatg ttgagtcacc ttaccgaatc
     7921 agcagcgaag aggaccatga aatcggcttt attattcagt agcaatcttt agattattat
     7981 ttgaaaaacg gagtttacaa gacaaagctg ctttattcta tatgtcatga acaagagagt
     8041 aaaaaagttc tcagatactt ttgttcatga ctattttctt caataaaacc aattgttatt
     8101 tttctgaaaa acaaag
//
LOCUS       DAZBTF010000103         7812 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000103 DAZBTF010000000
VERSION     DAZBTF010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.107"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(32..1384)
                     /gene="celB"
                     /locus_tag="ACHGNS_002613"
     CDS             complement(32..1384)
                     /gene="celB"
                     /locus_tag="ACHGNS_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFN6938505.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(1384..1902)
                     /locus_tag="ACHGNS_002614"
     CDS             complement(1384..1902)
                     /locus_tag="ACHGNS_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938506.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(1918..2244)
                     /locus_tag="ACHGNS_002615"
     CDS             complement(1918..2244)
                     /locus_tag="ACHGNS_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFN6938507.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(2257..4236)
                     /locus_tag="ACHGNS_002616"
     CDS             complement(2257..4236)
                     /locus_tag="ACHGNS_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFN6938508.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(4257..4571)
                     /locus_tag="ACHGNS_002617"
     CDS             complement(4257..4571)
                     /locus_tag="ACHGNS_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFN6938509.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4739..5032
                     /locus_tag="ACHGNS_002618"
     CDS             4739..5032
                     /locus_tag="ACHGNS_002618"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFN6938510.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(5110..6222)
                     /locus_tag="ACHGNS_002619"
     CDS             complement(5110..6222)
                     /locus_tag="ACHGNS_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFN6938511.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            6375..7223
                     /locus_tag="ACHGNS_002620"
                     /pseudo
     CDS             6375..7223
                     /locus_tag="ACHGNS_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7576..>7812)
                     /locus_tag="ACHGNS_002621"
     CDS             complement(7576..>7812)
                     /locus_tag="ACHGNS_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFN6938512.1"
                     /translation="DATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKH
                     LPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         2495 a         1554 c         1262 g         2501 t
ORIGIN      
        1 ttttttgtca cagttccttt tttcttagaa atcattttaa cttttcacta gctaattctg
       61 ctgccttttc tctttcttca tttaataatt tattatcata gaatttgata aatgggaacc
      121 agataatgaa tgcgatgatt gcacaaatga ctgctaacac agcagctttc caatcaccac
      181 cactaccaac aaatgctccg atcccgactg gtgaaggcca cggcatctgt gcaagcatcg
      241 gtttgacgat ttccagccga atggcaaaat aagcgagtgt agcagaagcc atcggagcta
      301 agaaaaacgg aaccgctaaa tagggattgt agacgattgg catcccgaaa atgattggtt
      361 cattgatatt gaataatcct ggtacaagtg aggcttttcc taacactttc agctgatcag
      421 atttcgcgag gaatgcaata aagatgatca atcctaaagt cgcacctgat ccaccaacgg
      481 ttacataaga attgttgaat tctcctgcca acggataatt tgcgccttgt gcgttagctg
      541 ccatattagc taatacgatc ggggtcaaga aagaagtgat gatattcgct ccatggatcc
      601 ctacgatcca caatgcatgg atcaagaaat aaatcaccat gacaccaagc catgtattcg
      661 tcaactgcgt aacaaaccca aatgggatag caatcatttt gaagatatcc gttcctagtg
      721 caactaataa tccattgatg atcatcacca caaacgcaat gacaaatgtt gggatcagag
      781 cagtgaaaga acgagaaact cctggcggaa caacatctgg catttttatg acccaatttc
      841 tcttgacaca catacaatac aattcgaccg caataatcga cataataata gctgtaaaga
      901 ttcctgaagt tcctaaacgt tcgacaaaag agcccatcct aataccattt actacagttt
      961 ctgtctcatt gatagaagca atcaagctga ttttaccatc agtcatgatc aattcaggga
     1021 tacacataaa gaatgcaaaa acagatagta atgcccccgt gagcggatta acatttaatt
     1081 tttgttcatc tcgttcaatt gacgttaatt cataagcaaa cacaatggca aagtaaatag
     1141 ataaaatccc catcgtcatt gtattgacga tcatgtacaa gtcactaact ttgaaaaaag
     1201 ttgcattaaa aaatccttct aaaaatggga acggtaatgg caaaatattt aaaaccaaaa
     1261 acatcgagcc tacaatcgtg aaaggaatag aagccatccc cgctgccatg acagcccgaa
     1321 cgattttaag ttgcgcaatt ttccccatag gtcccatcag gtatttctgc aaaaaatcaa
     1381 acattagtta ttctcctttc ctaaatataa cgctttttca tattgtttaa tacggatata
     1441 ctgttggtct tggttgagtg aaattttctt taaacgatac tgtataaatg cactggcaaa
     1501 aataccgctt acaacaagaa tgaaaacaaa taactgtcct ccattgttat ttgccataaa
     1561 aggatacaag aatgtaaatc ctgaggtaaa caacactgga atcactaata aattaaagac
     1621 actctggaac aaaaaatacc actttgtcca tggaacgttc gtttggtgtt ttcgataaag
     1681 ctttacttgt tctcctactg caggcaaaat agctaaaatc agaatcatcg gtaaactact
     1741 tcctaaactt ttagctgata gaagcagaat catccattgc atgttcatga aaaagaaaaa
     1801 ggcagtcagg taacgtacga ttagataacg attgaaatac atgtttttca aactaagttg
     1861 ctttttttct aattcgattt tcctttttac tgtatcctgc acaatgatcc ttcctttcta
     1921 tttatttttt tgatacaaat aattcatttc ctgtgcaact tccaataatg tcatcgtcgt
     1981 catcagatga tcttgtgcat ggaccatgat aatttccatt tctatttttg tcccgctggc
     2041 atattctttc agaagttgag tttgtgactg atgcgcatga agaatctctt catttgcttg
     2101 tcgcaacttc tcttctactt cttcaaacat ctctttacgc atcgagcgaa atgcttcatg
     2161 tatcagtgtc cgtgcatttc cgctatggaa aatgatatca aaagaagtca cttgtagtgt
     2221 ttcactgttt attttttcag ccatctgttt tcctccttat tcgatcagtt tgataaattt
     2281 actgctgaaa ttctcaaaaa taggttcagc taagatttcc tgctgtactt ttagctggtc
     2341 aactaaccta acgattgctt tagtcacgac cgtaattcct tcattttcga cataagaagg
     2401 cgaaactaaa aaaacaaaat gaatttcttg atattgatcc cactgcatcc cagaaggaat
     2461 caatgcaacg gctacctttg tttcgattcc aactggaaca gcgggatgtg ggactgccac
     2521 tgtatcactg aatatgattt gtcccatatt ttcacgttgc tggatctgct ttcgcatttc
     2581 tcttatatat ccttcttctt catttatgct cagcttttgc agcaaatcat gaagaatttc
     2641 ttcctttttc ggttcgtacg tataagtctt gaaaaatttt tcagacaact gttcggtgta
     2701 aatctgcttt ttttgctgta gagaaaatgc tggtatcggt ctatctgagg aatgataggg
     2761 tatagattct ccaactacct ttcgtatttt acgaacatca tcttcattca aaaaaatact
     2821 gacatgtaaa actggaattt tgaaaaacat tgtcgaaaga tcaatagaag aaataatcag
     2881 gtcgattcct tttaaagttg tttcattgat ctcataatag ccttttacac ttaccactgt
     2941 aatattttta ccgaattcac tgactacgcg attctttaat agttgggcac tgccgtaacc
     3001 cgttgcacag ataatcaatg catgtagttt atgcgcagct ctttcttttt ccaatgccgc
     3061 catgagatgc aaagccaaat aggcccattc atcttcattg atggtatagt ttttcaaagc
     3121 cggcatgttg ctgaaatgcc tttttactgc gtcaaagact ttgggatttt ctttctttat
     3181 ctcctcagtc aatgggttct caatgcgaat acctcggctc aatcgaatca tcatcggtga
     3241 caaatgatcg agtagtccat tgaaaagctg gagatcctct actaatgaaa aatgaaattc
     3301 ctgagacaat cttttcagaa ctttcgctaa ttcctttgat agttcctgat cattttgatt
     3361 ttctttgtga ttcgacttag ccatgagatg taatgtcaaa taagcaattt cttcttcggg
     3421 aaacgataca cctgcgatta cctcaatacg ttgaacgatc cgctgcgcca cttgatactc
     3481 tactcgctta gaaacttctt gttcgatgcc taattccttg atttcaaacc cttctcggag
     3541 ccgtttgatc cccaaagcca agtgtaaagt cagattctgt atgataaaat ccgaaacttt
     3601 taacttagct tctcttgttt catcgagaat gataatgact agctcttcaa aactgatgtc
     3661 tgggaaaaaa cggctttgcc ctaaatattc tttcaatgaa tttccataat ggtttccaaa
     3721 aaaagtatcc atgataaaat gccgtcgatt tctttcttcc ccttcgatcc atgtcccttg
     3781 accatgctta gaaacgattt ttagtgaata tggaatcaat cgctccttga tttcttgcat
     3841 gtcctttgtc aaactggaac gactgatata aagctcttct gccaaatcat ccataaaaag
     3901 aatggcatct tccaaaagca attggttcaa tatatacttt tgtcgatctt cggactcata
     3961 aaatccttgc ggttctctag catatccagg tactatttct tgccgactca aaaacaagtc
     4021 aaatgtcagt ctttgtaaaa tatgcagttg gtagccataa cctggtttag caatgatctt
     4081 acctccgttt ttttctacta actctttgat ccgctgcaag taattacgga ttgtccgatc
     4141 agataataac aattcagaag ctaaacgttg actggtgaca tactccttct tgtgatcaat
     4201 caaatatctc aacagcaatt tttccttctg tttcattatc atccactcct attccatcat
     4261 atgttctctt cgatcacctc tgctaacttc tggatgcctg tagggattgg gacatacgct
     4321 tggaaaggga tactaacaac aggctttcca acagattctc ctaatttttt gaatttatcc
     4381 aaaaacatcg tcgtttgcgg gctgatcaga aataaatcaa aatcactatt cttaatcata
     4441 ttatcgcctt ctgtagctga aactgcatct acgagtacct cttctctttt ttgcttaaag
     4501 aaatccgttg tctttgaggc catcatcgag gatgacattc ctgccgcaca aataatcaat
     4561 gcttttttca taaatcaatc actcctttta tttttataaa ccaagcatat cttgtaagcg
     4621 ttttctattc aaaatatata tttccgtagc attgcggaaa aaatgtgtat catatcttga
     4681 ataataattt ctggcaccgt gatatactag tgatagaatt ttttcaggag gggatttagt
     4741 gagtatcaca gtgaatattt tgtatacaag tacaaatgga caagccagaa aatttgcaga
     4801 agaaatggtc gaaagaaaca ttgtcaaagc gattcgtgca gaagaaggaa acgagaaata
     4861 tgactattac tttccactag aagatccaga atctctttta ctgatcgacc gttggaaaga
     4921 tgaagaagca atcgaaaagc atcacaaatc cgaaatgatg gctcaaataa acgaactccg
     4981 taaaaagtat aaattaaaaa tgaaagtcga acgtttcact gatttaccgt aaaaagatac
     5041 acatggaatg atagcaattt atcatccatg tgtatcttca ctttttcctt attttttatt
     5101 cgatctcatc taatgattca aaccgtgttt ttctcttatt tttcggataa gtatttcagt
     5161 caccccaata tacatgattc ctaaacatac cccaaggata ttctcaaaaa tgcggatcgt
     5221 tactgctcct ggaaccccga ataaactggc agtgattgcc aaagcaccaa aagaattaaa
     5281 aactgtttta tatctatagg tagaacaaac acctaaagct agaccaccta atataccgac
     5341 ccagttaaaa ggaataaatt ggctgatacc aataaataaa acacatccaa tcaatgagcc
     5401 aatgattcgg tctactgctc gttctttgaa accaatcgac atcaatccat agctagatac
     5461 aatagaagaa aatgcaaacc ctgcccacat caatcgctga aaagggacat actctcctgc
     5521 aacgatcaaa agactaatgc caaatgcata gatgagcatc caaatttttt cctgcgaata
     5581 catgcctttc aaaaaaatct ctttaaacaa gcttttgtct ttgttctttt ctcgatgttt
     5641 tctgtaaagg agaactgaaa accaacaaaa aaacaaaaat aataagctac ttgtttgtgt
     5701 aaagaaattc ttgttcaacg aatctttagg taacgaataa acaataaaga gatatgaaaa
     5761 gccgtacaaa gaagcattcc ccattttggg atcactagcg gttgccatta atatgctgct
     5821 tagtaaaata aaatgaatca acatgcccat aactgaccat ttgatcagct ggacatacgg
     5881 tgctactaat agtgagaaca tgacgattgc taaacctatg atcgaatggg aaatcttata
     5941 accaaagtca acaaaacgaa tactcatcaa catacagaat aaaaccacag cgtatggttt
     6001 atgcttcgat tcaaataatg ttgtaattat aatgataaaa caaatggcaa acaatacgat
     6061 gaataatgat cgtaaaaaca atgaacaaat atacatgcac ttttcttttt tagaagctgc
     6121 ttctcgtatt ttttgtttta aaataaacgg atctagttgt agagactgat aaaaatccac
     6181 ctgttgtcgt cctttctttt tacttttatt ttttacagtc atctatgaca ggttgtaaat
     6241 actatggaac atcatatggc gttgtcattt tcttgtcaac tatttttcct attcatgttt
     6301 ttataaaaaa acatatcgtt ttcttgtatg attaaatcac cacaaaataa acaactgaga
     6361 gaacgatatg ctttatgaat ggttctatca aaaaaattct gagatcaata gacaaaaatt
     6421 tgatgattac agaggtctct tacgaaacct tacagaagaa aaaaacattg atcgttgctg
     6481 cagttctctt ccctgctcca tgtgtctgta gaaattgtgg ttctgctgtg gtagatggaa
     6541 ataggaaatt aatggtggtg aaaaacaaga agaaggaaac cattgtccac tttgaataat
     6601 ataatcatat gccaatggtt atacgcctaa aaaagcagcg ctatacctgt aaaaactgtc
     6661 gaagccattg gaacgctcaa agttatttta tccgtccaag gcactcgatt tcaaatcatg
     6721 tcagacataa aatcacttct ttactgactg aaaaagtgtc gttatttttt atttcgaaaa
     6781 gttgttaggt atctttgaca accgttattc gcacattgaa agagttgaga agctatttac
     6841 caaagtcttc caaaagaatt ctgtcttgag tgttgagggt tgtgaatttc gttctcacgt
     6901 ttccgttgag gataaactga gttttatttc cgcggatggc gagacagaaa aattaatcga
     6961 gaaaatttac ctacacaaaa aattcctcga ttaacaaact atttccttag ctgcactaat
     7021 cctaaagaaa tagaattctt agttacagac atgaacgcca cctactttca gctcaccaaa
     7081 catgttctac caaatgcgaa attggtgatt gatcgattac ttggtttttc cacgcctttc
     7141 aaggaggcgt aacctttatt cacgagttgg ttgaagcttt ccgagacaaa agacccgact
     7201 tgtttttctc cttattgaca aagagccatt aaaactagag aaaaaattac aactgatcgc
     7261 tgattatttg tagaaagaaa atcaaagtat tacccaataa acttgagtca ttaggcaaaa
     7321 aacactgcga aactcaagaa tgattttcgc agtgtttttg tatatttttt attaactagt
     7381 attactacaa aggtttattt ttcacttctc aacgcagcac aaaattgtct ttacttattt
     7441 actggttggg ttcaaccttc tctactccga tactaatatt tgtttgtata ccccttctaa
     7501 aatcggtagt ttccattcaa ttgatgggta ctagttgatt ttaaaaacta tgcttattca
     7561 attttttatt ttagctcata tatttcgttt ctttctaaat tttatactga tgaaaacaag
     7621 tagcacaccc aatattgaca atataaactg gctaatgatg gtttctccag tttttggaag
     7681 atgcttattt ccactgcttg ttggattgtt tcctcttata tctgagctat cagcaacttt
     7741 tctattctct tttgttaaaa agatcgccac ttcttgttct ttttcgatct tgaaagttac
     7801 tggtgtcgca tc
//
LOCUS       DAZBTF010000104         7674 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000104 DAZBTF010000000
VERSION     DAZBTF010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.108"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..107
                     /locus_tag="ACHGNS_002622"
     CDS             <1..107
                     /locus_tag="ACHGNS_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HFN6938513.1"
                     /translation="NESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(176..904)
                     /locus_tag="ACHGNS_002623"
     CDS             complement(176..904)
                     /locus_tag="ACHGNS_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFN6938514.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(917..2197)
                     /locus_tag="ACHGNS_002624"
     CDS             complement(917..2197)
                     /locus_tag="ACHGNS_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFN6938515.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2338..2940)
                     /locus_tag="ACHGNS_002625"
     CDS             complement(2338..2940)
                     /locus_tag="ACHGNS_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFN6938516.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3185..3391
                     /locus_tag="ACHGNS_002626"
     CDS             3185..3391
                     /locus_tag="ACHGNS_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938517.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3703..4422
                     /locus_tag="ACHGNS_002627"
     CDS             3703..4422
                     /locus_tag="ACHGNS_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFN6938518.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4503..5186)
                     /locus_tag="ACHGNS_002628"
     CDS             complement(4503..5186)
                     /locus_tag="ACHGNS_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFN6938519.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5405..7591
                     /locus_tag="ACHGNS_002629"
     CDS             5405..7591
                     /locus_tag="ACHGNS_002629"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFN6938520.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2758 a         1293 c         1447 g         2176 t
ORIGIN      
        1 gaaatgaaag tcaagaagta ttgctgaaag aaatggagaa ttggccaaca ttttatccag
       61 atacaatgga tggacgtgaa attgcacagg aaatgctttt gcactgatag gcaaaaaaga
      121 accgaagggt ttggatcggc tggtcattta gtcggtccaa actattcggt tctttttatg
      181 aaatcaattt ctttaatcca cggatagagg ctgggatggt ctcgccgttt gccaacgtga
      241 cttctctggt ttttttatta atcgaccgca cattttgtgt attgataaca taggatttat
      301 gacaacggat aaaattccga tgcattttct cgatttcttt gatcttctca tagaattgta
      361 attgggcatg agtcaaatgg acttccagtt tatgtggcgc tcctagcgaa gtagaaaaat
      421 attggatgtc tttgatcggt aaaaatttga tctcatcatt aaactctatt ttgaagatat
      481 ctttttggat ttgggacatt tgaaaatctt cgaaaatttt gctcaggata tgctcgacat
      541 tttcttttaa atcataaaga tcttctttga cgatataatc agtcggttcg atgttgcttt
      601 ttaagatttt catcgacatt tccgtatggg aagtcacata gattattttt cctaaaggat
      661 ctaattcacg gatcaattta ccgatcgcta caccatccat ttcactgtct tctagttcga
      721 tatctaaaaa gtaaatacca aaagaagaag ttctgatctt ggctgcattg agaagttctt
      781 ccggatcaga ggtagaaata tggacataac tgtctaagtt ttcaatcatg attctatttt
      841 tgatcaccgt tgataaaact tctaattgtt ttggatcgtc ttcacaaata taaatgggaa
      901 tcatatcatt actcctttat tttatttgta tataaatcgt ttgagaaaaa cgctggtttt
      961 caattgatgt ttcaaggaaa agataaggat tggtctttgt caattcatcc aagataaaca
     1021 agccaaggcc ttcgtttttt ggatttgttt ttgttgagaa ccctttttgt ttcaactggt
     1081 ttaacggtaa tgtatttgtt gttgtattga tcacacgaat catcacggaa tgattcttct
     1141 tgagtatggt caattcgatt tttttcttct ttgcagcact agctccttca atcgcattgt
     1201 ccaagataat accaagcata cgtacaaggt ttaccgtatg tttcgtatcc agttcgattg
     1261 gctcaggaaa atgaagctgg acgatcaacc ctttttcctg tgccatcatg agttttaatg
     1321 acagtaaact acggatctca ggaactttca tattatccaa catacttaga ttggccggaa
     1381 aaaggttcat catctggcgt gtaggtaata tcgtttgatc ataataagac cgtaatcctt
     1441 ctaagtcgcc ttctgaaata tagctgtgaa gagaaaaaag cagattttgg taatcatgtt
     1501 tgaattctct caagtcttga tagttttttt ctagatcttt gatataatcc tgttgcatcc
     1561 tgacttgttg ctctaataac tgatcagcta atttcttatt agagattttc caataataaa
     1621 ccaagatgat caggccaaca cacagatata gaatgaaaaa actcgtattc aaagctaaga
     1681 catcaggtgt ttcaccagaa aaacgggtat aaaaaataat gatatagtat gtaaataata
     1741 tcaatgtccc gatcgtaccg atcatacggc ggtccacagt gtgaaaccaa gtctctaacg
     1801 atttttttaa acaaaaagct aataaagcag ataaaaaagc ggataagaca atgtgaaaat
     1861 actgcaatga atctcctggt tcaataaccg aagatttcaa aacaaaaaaa tcgccgatgc
     1921 ttgttaagtg atctcctaaa agcgacgtca ataacccaaa aggaagcgcg taaaccaatt
     1981 gttgacgcga agtcgaaaag aagaaaaaag taagcatcag cattcctata gcaattagag
     2041 cagtaaaaat ccctaaaatc gaatagaccc atccaatcaa aatacaagaa atcagaatga
     2101 ctcccgcaaa cagatatttg tatcggatgt gatggtcaat caataagata caaattgtgt
     2161 aaatcaaata ttgcaacgct atggaccata aaaacataaa gtacagctcc cattgatttt
     2221 taccaaatta tagcacaatt ggcgtattat ttttttaatt tttcagggat tttgaattca
     2281 gaactcatac caaagcagca gccagataca aaaaagtcag ataagttagt taattgttta
     2341 atgattgttt ttttcatgaa agattcctcc aaaattcatc aagtagtttg taataggaag
     2401 caacgtagct atttctaaaa caatcccaag tagcaaatag gtagaaaagt aagaagctga
     2461 gaaaaataac gtaatgagag aaaaaattag aaacatgctg atcgccttct ttaataatgg
     2521 gggacgtttc tcgggttcaa tgggattgat tgctgtacct cttgatccat agcgaagcag
     2581 taacaaaaag cagctgatta acaaaaagac catcattggg aaggagatag tcaattgcag
     2641 atgtttcatc accaagggaa cgccccataa ataaagtagt gtccacagca gacaaacgaa
     2701 ttcagagtcg gaatgcaatc cataagcata tctgcggatg cagatataag aaagaaaggt
     2761 gattcctgtt tccgcccatg agcctgtcac cagagaaaag actagaacga ccgctagttt
     2821 tccaagatta ttcaataaaa cttctaagac aaattttact tggacataca ccatatcttc
     2881 ttccgatttg tctccgctga ataaggcttc taataatttg ttcacaactt tatctttcat
     2941 ctcatacgta cctccaccac aaattttatg ctaaaaaaaa taaaaaacgt cttttgtttc
     3001 ataaaaagtg gttttgacat caaaaccgtc atgatctcct aaaaaaatct tcgttttcca
     3061 agaaccttac caaaaaaagt gacagttttg atacgaaaag tactttttat gacaagtttg
     3121 tgctttttcg agtgatttat gttaaaaaat tactgacctt ataaaaggtg atggagggat
     3181 tcttatggaa aatgaacaat tgtttgaagt cggcgaaaca tatgcattta aatctgattt
     3241 atttgaatca ccaataaaag gaaaaataga aaaaatctat gaaaatagtg tgatgatacg
     3301 tgtcatgaaa tgcgcagtag aagacaatga taccgctcat aatctgcact ggcagttagt
     3361 tgtacgcaaa tcaagtacaa tagaagaata aaaacagata aaatgttgag agcataaaga
     3421 ggttgtgagt ctcccgttta gctctgagtt acaaggaata atgttaaaaa acagcttttc
     3481 atgttttatg aatattgcct tgtgaccgag gtgttggcag gagaatactg tttatttgaa
     3541 aaaagggctg cgacaagact tttgtcacag cctttatttt catgaatagg agttactttt
     3601 ggctactttt agagaagtag cttttaagga gcagataaag tcaaggcttt tatttctata
     3661 aatgaagtag taaaatgaag aacagatttt attacaggaa ggatgccgat aattgtgatc
     3721 attgttgcag gaacgatcgg tgcagggaaa agcacactaa ctgaaatgct tgcccaagat
     3781 ttagaaacaa aaccttttta tgagaacgtg gaagataatg aagtattgcc gctattttat
     3841 tctaatccag aaaagtatac ttttttatta cagattttct ttttgaacaa acgctttttg
     3901 gcaatcaaag atgctttcag ccatgatgac aacgtactag accgctccat ctacgaagat
     3961 agtatgctgt ttcatttgaa tgcagattta gggcgtgttt ctgaagtaga agtaaagcag
     4021 tatgaaggtc ttttagaaac gatgctgaag gagttagaag aaatttcgcc acaaaagaaa
     4081 ccagatctac tggtttatat tcgtgtatcc tttgaaacta tgttagcgag aatcaaaaaa
     4141 agaggccggg aatatgaaca gctagaacaa gatcctgaac tctattccta ctataaagaa
     4201 ctcaatcgac gatacgaaga gtggtatgaa cagtttgata tatgtcccaa aatagtgatc
     4261 gatggggaca aatatgattt cgtagccgat ccggcatgtg gagaacaaat cgtacaagaa
     4321 atcaagatgc gggcaaagaa aatgacagaa gaggcagatg cccagtcgat ccgctacact
     4381 acaaataacc cgaaagcaaa gggaatcttt acaactaaat aatataaaaa ctaagcaaat
     4441 tgctcactcg cagatatttc tctgtgagca gcatcttgct tagttttttt gattcattga
     4501 gatcaaatag tgaaccgatt cgtatgataa aattgccata aatgaaatgt ctgagtgagg
     4561 gccatgatca aaaataaaat ggaaacgatc aaaatcgaat gggcgccaaa cagatgatga
     4621 cagaaaacaa ggacaatagc tcctgcgata aaggcaagaa cgacacttag atagtggaaa
     4681 aatcgatcaa aatctgcttt ttctttagaa atacaataat catataagtt cgttgctacc
     4741 ccttttaaat ttccagttgt aaataaatta gtgtaggcac gcccgttgat tttatcaaaa
     4801 gctacccact ggatcgatgc agtaaaagca atcaaaatcg ttactaatgc gggatgctca
     4861 tggacgggtg tcaaactaac aaagaaaaaa atcactgctt cataatatag aacaaacaag
     4921 cgccagaaat gtttttcttt ttttctaaaa taatcaatta gatactttgt tagaagaatc
     4981 cctaagaaaa agaaaagggt agacaatact tttttaccaa cacttagcca ttctccgtca
     5041 aatgcttgga tgatcgatag aatgatattg ccagtttgag cagaggcaaa agcaccatat
     5101 tggatataag tataactgtc catggctcct ccgataaatg tcagcaaaag tcctattgta
     5161 cgcccctcgt gaaaggaagt ctgcattttt ttcatcacct tttatgaata tttttgtctt
     5221 ccttatctta gcataaacga ttgaaaaaaa aggaaaaatt ttcagaaaac taaaatcata
     5281 tcgcttccca ttttcatttt tagaaaggtc tgttagtata aagaaaaaat agacaacgaa
     5341 actttaaata accaacttga aataaacaaa ttcaaagaag aaagatggga aggaaatcaa
     5401 tcagttgaat gaaaaagaaa ttctccgatt acaaaaagaa ttgaaacgct gggagaacaa
     5461 gtataaaaag cttcaacaag tctttcggct acaagaagaa atcattgcta cctatgaagc
     5521 ggcttatggc gaagcacccg gacttattga ggaagaagta caagtaaaag aaagtgaagg
     5581 aaacagctct ttctttaaaa tgatggagaa acgttcctat aatcgcttca atcaagaaca
     5641 aaaagcagct atcacatatg atatgaagaa acatcttcga atcatagcag gagctgggag
     5701 cggcaaaacg caaacaatct gtgcaaaagc tgtttatctg atgacgcaaa aacaagtaga
     5761 cgaagaacgc atactaatga tcacttttac aaggaacgct gcaaatgaac taaaaaaacg
     5821 agtagataat ttcagccaga gaaaaaccgc tgtccatatc ggaacattcc atagtatctt
     5881 ttttaggata tacaatgaaa tttgtcgaaa atttcctgaa gtagcaatgc aaggaataca
     5941 aggagacttt tcaaaggata ctgcacaaaa agtgaatgct gttttgcaac agctgatacg
     6001 taaatataat ctttatatat ttgatcaata tggagaaaaa acgattgctt ctcggttaga
     6061 ctattggcag aatatgaacc tttcagtaga agaaatggtt cagttagtta aagaaaaata
     6121 tgattccatt gataagaatg cacgacagcc gatatcagaa cgactgtatg gattgctcac
     6181 ggaactccaa gaacaaaaaa gaagccagca attgctagag ttcaatgatg tactccaaaa
     6241 tctgaaaagc gctttggaga atgaggagat ccagcattat atcggccaaa aatatgatta
     6301 tctattcatt gatgaatttc aagatactaa tccacttcaa tgggaaatcg tgaaacggat
     6361 gacaaaggaa aacaggatca agttgattgt tgttggagat gatgatcaga gtatctatgg
     6421 attcagagga tcagagccag catacatcaa gaactttgaa aaagaatatc ctaccaaaac
     6481 tcttttctta ttaactaatt atcgttctcg ggcagctatt gtccaaggag caaatagatt
     6541 gatcagctat aataaaaatg atcggatacc aaaggcgatg attccagcac aaaaagaagc
     6601 aggtgttatg gaagcctatt tgttttctga tactaaagca gaaagtcagt ggctaatcaa
     6661 acaaattcag acatttattc aaaaaaatgg gaaatataag gaatcaatca tattataccg
     6721 atcagcttca caaacgcagc agcttgtcca atctctgctg aaaacgaata ttccttttgt
     6781 actggaagca gatagtccat atgaaggtat ctttggtatc aagtctttcc aacatttcta
     6841 tcaaaaaatt gttcattggc aaacagcact gaatctgcaa aagaaaaatc aggcctacca
     6901 gcaattgctc cggcagctga tggcagactg ctacctgaaa aaaagtgaat gcgatcaata
     6961 cttttctcct aaatatcaaa atatagccgt tgcagattat attttgtcga taagccccaa
     7021 tttaaaatcg aaatctgcag aaatccgaga gtttgaggaa gcgttgcgta aaacagacca
     7081 gcatcaagaa atctattcgc ttgttcaaag ctatcttcat ctgccacgta tagctaagga
     7141 aatcgaccag agtgatcaag aatggatacg agaagaagta aagcagcatg ctactttcag
     7201 aagactccaa gccttatcgc aagaaaccca agcgacagct aaagaattaa aaacccgctt
     7261 gattgcttac agacaaggaa aacttgatgc actatgtatc cagagcattc acaaaagtaa
     7321 aggtcttagt taccgaaatg tctttttgat tggttgtaac gaaggaagtt tgccttataa
     7381 cggagcgaca gaaaagaaag taatcgactt taatgaaata aaagcagaac cagtaacgac
     7441 gatagaagaa gaacgaagac tattttatgt tgcgatgact agagcaagaa atcgtctgta
     7501 tctatgtgta cctcaaatga aaaattcaag aaaattgaaa gtatcgagat ttatcaaaga
     7561 aaccgggagt cgtgtgaaaa aaggaaagtg atagaagaga gggtgtgaca gaagtggtca
     7621 gtttcaagaa agaagaagga atgtacgaaa attgcttctc aaatttttgt gaaa
//
LOCUS       DAZBTF010000105         7600 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000105 DAZBTF010000000
VERSION     DAZBTF010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.109"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(138..428)
                     /locus_tag="ACHGNS_002630"
     CDS             complement(138..428)
                     /locus_tag="ACHGNS_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938521.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="ACHGNS_002631"
     CDS             complement(546..806)
                     /locus_tag="ACHGNS_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938522.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="ACHGNS_002632"
     CDS             complement(943..1356)
                     /locus_tag="ACHGNS_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFN6938523.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="ACHGNS_002633"
     CDS             complement(1812..2297)
                     /locus_tag="ACHGNS_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938524.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="ACHGNS_002634"
     CDS             complement(2436..2651)
                     /locus_tag="ACHGNS_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFN6938525.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="ACHGNS_002635"
     CDS             2875..3675
                     /locus_tag="ACHGNS_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN6938526.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="ACHGNS_002636"
     CDS             3694..5562
                     /locus_tag="ACHGNS_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFN6938527.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="ACHGNS_002637"
     CDS             5555..6046
                     /locus_tag="ACHGNS_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFN6938528.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /locus_tag="ACHGNS_002638"
     CDS             6034..6588
                     /locus_tag="ACHGNS_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFN6938529.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..>7600
                     /locus_tag="ACHGNS_002639"
     CDS             6606..>7600
                     /locus_tag="ACHGNS_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFN6938530.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1213 c         1443 g         2237 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacatagaag aagggatata taaagtaatg aaacatttca agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgtttccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg
//
LOCUS       DAZBTF010000106         7292 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000106 DAZBTF010000000
VERSION     DAZBTF010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.111"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..306)
                     /locus_tag="ACHGNS_002640"
     CDS             complement(<1..306)
                     /locus_tag="ACHGNS_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN6938531.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     "
     gene            485..2131
                     /locus_tag="ACHGNS_002641"
     CDS             485..2131
                     /locus_tag="ACHGNS_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFN6938532.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            2180..2899
                     /locus_tag="ACHGNS_002642"
     CDS             2180..2899
                     /locus_tag="ACHGNS_002642"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFN6938533.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            3378..4325
                     /locus_tag="ACHGNS_002643"
     CDS             3378..4325
                     /locus_tag="ACHGNS_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6938534.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            4337..5257
                     /locus_tag="ACHGNS_002644"
     CDS             4337..5257
                     /locus_tag="ACHGNS_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6938535.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            5298..6755
                     /locus_tag="ACHGNS_002645"
     CDS             5298..6755
                     /locus_tag="ACHGNS_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN6938536.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            7100..>7292
                     /locus_tag="ACHGNS_002646"
     CDS             7100..>7292
                     /locus_tag="ACHGNS_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFN6938537.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWN"
BASE COUNT         2284 a         1265 c         1511 g         2232 t
ORIGIN      
        1 gaaatgtgag gagatcattt ccacttttgg aacaatctct aaaacaacag gaaaaagcag
       61 acttcccgct acagtcttaa aaaatactga ttttcctaat aggacagcgg ctagaagcag
      121 catcataaga ttgatgctcc aaacagtaac tgagcgttgg atagaaaagg ccgtttcaac
      181 taaatgtccg atggcgccca ctccggcgga agctatatcg tgagggagca agaaccaatt
      241 caatccaact gtgataaaga aaagacctaa aaatataacg ggtagttttt ttatttcttg
      301 tgtcattgat atctcctata aatgttagcc ataaaaggct gtatatttct ttactttacc
      361 aaaaaaacga aaccgaattc aaaaaaacaa ttgttcttat aaaagttata taaaatcgat
      421 tgccagtttc tgttttacca attaaaatga tagtaatgtt ttgtagaaag ttggaatctg
      481 accgatggat aatcaaagaa aaccagcatt agatcaatta acaccgcaag aaaaaatggc
      541 acgaggctct gcttggatga ccgccagtaa tatcatttca agattgctag gcgctgtcta
      601 tatcattccc tggtatgctt ggatgggcga aaatgctaag gctgccaatg gattattcaa
      661 tatgggatac aatatatatg cattgtttct actgatctct acagcaggaa tacctgctgc
      721 gattgcaaaa caaactgcac gctataattc attgaatgaa tatggcactt cacgacgatt
      781 atttatccgt gcattacaga tgatgggcgg gctaggcctg ttgtttgctt tgttcatgta
      841 catcgcctct ccttggctag ctcatgcttc tggtggcgga gaagaactga ttccaacgat
      901 gcgttctttg agtatcgctg tattggtttt tccttgtatg agtgtgatac gagggtattt
      961 tcaaggcaat caagaaatga tgccttatgc gctgtcgcag attgtagaac aggtggcacg
     1021 tattttttat atgctattgg ctacattcat catcatgaaa gtcatggatg gtgactatgt
     1081 gacggcggtc acgcaatcaa ctttcgcggc atttatcggt atgctagcaa gtattcttgt
     1141 ccttttgtat tttttgaaga aacatcaagc gtatacatcg gcgtttattc actacagcga
     1201 aaacaaaata aatattgcaa cgaaggaatt gttgttagat accgttaaag aagcgattcc
     1261 gtttattatt gtgggttcag gggtaacgat cttcaaatta gtcgatcagt tcacttttat
     1321 gaagatcatg tctgcatcca ccgagtattc caatgcacag ttactggatc ttttttcgat
     1381 ttttagtgcc aatccagata agttgacgat ggtagttatt gcattggcaa catcgatcgc
     1441 gtctactggg ttaccgttga ttacagaagc tgttacgttg aaagatcgag taggtctggc
     1501 aaaattgaca agcggaaatt tacagttgtt ttcatttttt atgtttccgg cgacatttgg
     1561 tgttatgtta cttgcctatc cgctaaatac attattttat acaccggatt ctctaggaag
     1621 taatgtactg atccaagcaa gttttgtagg actcttccta ggactatata tgctagtttc
     1681 caacatgctg caaggaatgt ttgaaaacaa ggcggcaatt aattatttgg ttattgggtt
     1741 tctagtgaaa ctccttcttc aatacccagc cattcgttta tttgaagtat acggcccttt
     1801 attggcgaca atgatcggat ttgctgtgtc ttgtaccttg atcttaaaga gaatccatga
     1861 agtagcccgc tttaaaccaa gttttgtctg gcgcaggaca ttgctgatct ttattcttac
     1921 cttgattatg cttttggctt cgtggatcac aaagatgatt tttggtgctt ttttaaacga
     1981 agatagcaaa ttccagtctc tattgttgat tcttgtagta gcaggtatag gggctttagt
     2041 gtatggttat ttagctttga aaatccgctt ggcagaaaga ctactaggac caggaatagc
     2101 gcgtttacga cataaattaa aaataaaata agtgaatcag aagagctaga ttgatgctct
     2161 tttgaaatgg gggaaacgca tgcgtttaga taagttttta gcagatatgg gacttggcag
     2221 ccgcaaagaa gtcaaaggat tgatcaaaaa aggaacgatc gaagtcaatg gacaagtggt
     2281 gaaaagcgat aaagcgcaag taaatgaatt cgaagatgcg gttacttatt taggagaaat
     2341 cattacgtat caaaaagatt tttactacat tttgcataaa cctgcaggag tcatttctgc
     2401 tactcaggat aatcatgatc aaaccgtaat cgatttattg gaagaccagg attatcggga
     2461 agatcttttt ccagtcggcc gattagataa agacacagag ggcctcttga ttttaacgaa
     2521 tgacggggtg tttgcccatc agttactatc acctaaaaaa catgtggaga aagaatatct
     2581 agctgaggtt agcggtataa tgacagaaga agatatccgt ttatttgcag aaggactgat
     2641 tattgatggc gatgagcaga cattgccggc aaaactccaa attgatcaag tagatcaaga
     2701 gacagaaact tcaattatcc gtctgatttt gcatgaagga aagtttcacc aagttaagcg
     2761 aatggtcaaa gcagtaggaa aagaagtctt atatttaaaa agaatccgta tgggaaaatt
     2821 ttggctgcct aaagatttga ataaagggca atatcgaccc atgaccaaag aagagctaga
     2881 acaagtgaga gaaaaataat tgaatggata gctaaaagaa taagcagagt agttgtagcg
     2941 ccatctgaaa agcagacttg tccgtctgac tttcggatgg cttttttgtg aaatttcggc
     3001 ttatttccgt agaagctact tctggaacac cgtttgttcg gaataaggga ctgtgacaag
     3061 acttttgtta cagtccccat tgttccttgt gactcagagt tagatagtaa ggacacgatc
     3121 tccctaatac tcaggccaaa acgctgaatt cacaacctct gataaacggt gttcaatcag
     3181 ctgaccctcg gtattagctc aaaaaacggc aaaatatgag gaactatttt cccgtttttt
     3241 tctctaatac tcaggccaaa acgctgattt cacaacctct cctatacttt gtttcatttt
     3301 ccctatactt tgtttattta ttctcattat tgaaagcgct ataattacgt tgcgaatgaa
     3361 tcgaaagggg tttttagatg aaaaaacgaa aggggttcaa tcattttttt atgaatctat
     3421 ggcgttataa agcacttgtc ctgatggctg taccgggtat gctttggatg atttttttct
     3481 tttacatacc tgtaatggcg aatgtcgtag cattcaaaaa ttttcatatt tcagcagatg
     3541 ggttcttggc tagcttgagg gaaagtcctt gggtgggttg ggaaaatttc aaatttttat
     3601 tttcttcgga tcaagcattt ctcatcacta aaaatacaat tttatataat gtagcattta
     3661 tccttttgaa tctgctgatc tcagtcgtat ttgcaatcat tatgagcgag ttgagaaata
     3721 aacgactcgt taaagtttat caaacgatgt ccttactacc ttattttcta tcttgggtca
     3781 ttatcagtta ctttgtttac gcttttttaa gtcctgacaa aggaatattc aatcagtgga
     3841 tcactggaca tggaggagaa gctgtcaatt ggtataatga accgaaatat tggccgttta
     3901 tcttggtgtt cattggtaca tggaaaggga ttggttataa cagcattatt tactttgcat
     3961 ctgttatggg aatcgatccg acttattatg aagctgctat ggttgatgga gctagcaaat
     4021 ggcagcagat caaacatgtg acgataccac aacttattcc actaatgacg attttaacga
     4081 ttttagccgt aggaaacatt ttccgagctg attttggcct gttttataac attcctagga
     4141 attccggtgc attatatgat gtgactcaag ttttggatac gtatatctac aatggcctga
     4201 catccacagg agattttggt atgacggctg ctgcaggact ttaccaatca gttgtcggct
     4261 ttgtcttgtt gatgatcaca aatacgatcg cccgacgttt tgatagcgat tctgcattat
     4321 tctaaggagg aaaaaaatga aaaaaaagaa agtggagaga gtccaagtcc gctctttcaa
     4381 taagacggct aattttttct ctaatataat gattgcactc tttgctattt cctgtatttt
     4441 gccatttata tttgttatcg ccatctcttt taccgatgag tccatattgt cgactcacgg
     4501 gtacagtttt tggccagaaa agttcagtat atttggttat acgtacttgt tcgaccagat
     4561 gcaggataaa attttccaag cgcttttcgt cactgtactt gttactgttt taggtacact
     4621 gatcaatagc acggctactt ctttatatgc ttatgcgatt tcacggtcaa actttccatt
     4681 ccgtcgtttt tttaccgttt tttgtttaat tacgatgttg ttctcaccag ggatggtggc
     4741 caattatctt gttatgacaa atcttcttca gttaaaagat acgatttggg cattgatctt
     4801 gccaatggct gtcagtccct tcaatattat tgtgatgcgc acattcttta aacgttcggt
     4861 ctctgattcg attattgaat cggcacggat cgatggagct agcgaactgc gaatatttgt
     4921 tcagatcgtt ttaccgttag ctgttccagg tattgcgaca atcagtttat ttgccgcatt
     4981 gggttactgg aatgactggt tcaatgcttt actatacatc caagatgata agctagttcc
     5041 actacagtat cttttgatga aaatccaaag taatattcaa tatctgacac aaaattcagg
     5101 agcaggaagc caattgacag gcggattagc ctcagttcca ggagaatctg ccagaatggc
     5161 gatcgtagtc atctcgacgt tgcctattgc gatcagctat ccatttttcc agaagcattt
     5221 tgtcaaaggt ctaacaatcg gcggagtaaa agaataaaga aacaaaaata taaatcataa
     5281 aattaggagg aagaaaaatg aagttatgga agaaactagc ctttacaggt gtttcagcgt
     5341 tgttgttggg aacattagca gcatgcggag gttctggttc taaagaccag gcagaagcta
     5401 gcaattcgga cactttgcag atgtatcaaa tcggagataa accagataat ttcgatcaat
     5461 tgatggaagt agcaaacaaa cgaatcgaaa aagagatcgg tgtaaaagtc aatatcaatt
     5521 acattggctg gggcgattac gaaaagaaaa tgaacgtcat catttcttcc ggggaaaatt
     5581 atgatatcgc ctttgcgaac aactatgttt ccaatgccca aaaaggagcc tttgctgatt
     5641 tgactgaatt agcgcctaag tacgctgaaa aagcttataa tgatttagat gaagcatata
     5701 ttaaaggaaa tctggtcaat ggaaaattat atgctttccc agtcaatgga aatgtctttg
     5761 ctcaacaagt acttactttt aacaaagctt tactagataa atacgatctt tcaattgatg
     5821 gaatccaatc ttacgctgat gcagaaaaag tcttgaagga attccatgaa aaagaaccaa
     5881 acacggcagc ttttgccatt ggacaaggct tcaaagtcca agggaacttt gattatcctt
     5941 tagggaatac gttaccattt gctattgatc tggatggaga tgattcgaaa atcatcaacc
     6001 aatacgataa tgaaacgtat atcggactgt tacgtacaat gcatagctgg tacaaacaag
     6061 gactcattcc aagtgacgct gcaacgagca ataaggatta tccactagaa ggaaatactt
     6121 ggctaatgcg tggagaaaca caaggaccgt atgattatgg cgatacaatt ttgacaaatg
     6181 cagcaaaaca agaacttgtc tcaaaagcaa ttactgttcc actaaaatct acagctcaag
     6241 cacaaatggc aaattttgtc gtatcaaata cttcaaaaaa taaagaaaaa tcagtagaac
     6301 ttctagggct gcttaacagt gatccagaat tattgaatgg gcttgtttgg gggatcgagg
     6361 gagaagcatg ggaaaaagtc gatggttcag atgataaaat caaattgcta gatggttatc
     6421 agccaaatac acatatgtct gcttggaata caggaaacaa caagattcta tatacacaag
     6481 agtctattac ggatgacatg attgccaaac gtgatcaatc gattgctgat gcagaaactt
     6541 caccgatttt aggattcagc ttcaacactg acagtgtcaa aacagaatta agtaatattt
     6601 ctaatgtcat gaatcaatat ttagatggat tgaatacagg gactgttgat ccagatgaaa
     6661 cattaccgaa attgaaagac gctttggaca aggctggcta tgataaggta ttgaaagaaa
     6721 tgcaaaaaca atatgatgag ttccgacaag aataataatg aataaagaca aaggggttgt
     6781 gacaaaagac gtgtcacaac ctcttgttcc gaataaacgg tgttcaaaag ccagtttctc
     6841 ggtattaagc taaatatatg taaaaatgtg gaaagccata ttcacaaaat ttttcttact
     6901 actcgaaact aagcggctat ttcacaaact cttggtaaac ggtgttcaat cagctaaccc
     6961 tcggtattcg ctcacaaaac gacaaaatat gaggaactat ttttccgttt gtttctctaa
     7021 taatcaggtc aaggtgctga ttgcccaacc tttttttgta taatgagaaa gtataaactt
     7081 aatgtttgga ggagaagata tgttagggaa ggttttggaa gagttattta tccggatctg
     7141 ggtcattata aaactaacct tgtatttctg gatatataca tttgccggcg ggatcatctt
     7201 tggattaggt gcggcatgga aaacagtcaa tgaactattt tatttgtacg gattcgagta
     7261 taaagaaatc acaataaaaa gaggctggaa ta
//
LOCUS       DAZBTF010000107         7141 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000107 DAZBTF010000000
VERSION     DAZBTF010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.112"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..615
                     /locus_tag="ACHGNS_002647"
     CDS             <1..615
                     /locus_tag="ACHGNS_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFN6938538.1"
                     /translation="FVFAPLEGEDLASFLVGAAIMMIGMTLFLFGADYSMMEVGDLVG
                     KYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQQVYDISDGEIGRVLLIGIVSVGT
                     GVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFTSSEFMPIAFDSGGVTTGPVTVPF
                     ILALAGGMTSMIKQKKNENDSFGMVGIASLGPILAVMILGVIFQ"
     gene            612..1385
                     /locus_tag="ACHGNS_002648"
     CDS             612..1385
                     /locus_tag="ACHGNS_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFN6938539.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            1382..1780
                     /locus_tag="ACHGNS_002649"
     CDS             1382..1780
                     /locus_tag="ACHGNS_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFN6938540.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            1965..2636
                     /locus_tag="ACHGNS_002650"
     CDS             1965..2636
                     /locus_tag="ACHGNS_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938541.1"
                     /translation="MNFLVVLKDTLIERFTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            2695..3135
                     /locus_tag="ACHGNS_002651"
     CDS             2695..3135
                     /locus_tag="ACHGNS_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938542.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            3147..3815
                     /locus_tag="ACHGNS_002652"
     CDS             3147..3815
                     /locus_tag="ACHGNS_002652"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFN6938543.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            3879..4502
                     /locus_tag="ACHGNS_002653"
     CDS             3879..4502
                     /locus_tag="ACHGNS_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFN6938544.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            4558..4944
                     /locus_tag="ACHGNS_002654"
     CDS             4558..4944
                     /locus_tag="ACHGNS_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFN6938545.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            5157..6548
                     /locus_tag="ACHGNS_002655"
     CDS             5157..6548
                     /locus_tag="ACHGNS_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFN6938546.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         2277 a         1160 c         1572 g         2132 t
ORIGIN      
        1 tttgtttttg caccgttaga aggagaagac ctcgcttcat ttctggttgg tgcggcaatc
       61 atgatgatcg gaatgacgct ctttttgttt ggagcagatt attctatgat ggaagtcgga
      121 gatctagtag ggaaatatat gatcaaaaag aaaagtttga ccgttttggt cagtttggga
      181 tttgctatcg ggatcgtgat cacgattgct gaaccttccg tgcaagtttt gggacaacaa
      241 gtatatgata tatctgacgg agagattggc agagtgttgc tgattgggat cgtcagcgtc
      301 ggcactggag tatttctagc ctttgcgctg cttcgggtcg tattcaagct atcctattat
      361 caattgatgg ctataggcta cataggcgtt ttgatcgcct catttttcac cagttccgaa
      421 tttatgccga ttgcttttga ttcgggggga gtgactactg gtccagtaac tgttccattt
      481 atcttggccc ttgccggagg gatgactagt atgatcaaac aaaagaaaaa tgaaaatgac
      541 agttttggaa tggtcggtat tgcttcgtta gggccgattc ttgccgtgat gattttgggg
      601 gtgatcttcc aatgagtttc tggcagcaag tgatagatgg aatcgaaatc gttaccatgg
      661 aagtcatttt ggcagtggca cctatcgtaa tcctatttgt tttaatgaat atcattagtt
      721 ttcgtatgcg caaaaagcgt ttctttgaca ttatgaaagg tttcttgata acgactattg
      781 ggttgatcct tttcctgcac ggagtcaata tggcttatgt accggtagga cagcatttag
      841 gcagtgctat tgcgggattg ccttacaatt atatactggt tccccttggc tttttaatgg
      901 ggttcttagt agggtttgct gagccggcga ttcatgtaat gatcaaacag gttgaagaat
      961 tgagcgaggg gagaatccga tcgaaaatca tgctgatggt aatttcaatt gggatcggcg
     1021 cagcagtagg attgtctatg tggcgtttgc tcgtcggatt ctctctttat tatttcttga
     1081 tcccaggata cgggctagca tttattttag gacgaaaagt ggacaagatc tttttagcaa
     1141 tggcttttga taacggtgga gtagcaacgg gcccgatgtg ttcgacattt attttatcaa
     1201 tgagcgtggc aatcgctgca caaattgaag gaagagatcc gatcattgac gggtttggtg
     1261 tggttgcaat gattgcattg acaccaatat tatccacttt agtactagga tatatttata
     1321 aacgaaaaga tgaaaaagac agaatcgaaa aagaaagatt gcaaaaattg ggggaaagca
     1381 aatgaaagat cttgtagcat tgaatcttca catgattgtg acgatcgttg acggaggaat
     1441 gggcgaagat gttatcgact tttcaaaaga agcaggtgca gcaggcggaa cgatcttaca
     1501 tggaagaggt tctggtgtac atgattcagg gaaattttta ggtttggaca tttcgccaga
     1561 aaaagatatt gtgttgacat tagtacccga tagtttgacg aataaagtaa tggatgcgat
     1621 tggtcaaggc atcaacatcg atattccagg taatggtatt tgttttagta ttgatgtaga
     1681 caaagtcatt ggtatcacta agattcatca atatcgagaa attatcggaa tgcaagaact
     1741 tttagatgaa aataacgata gagaaaagtc tgaatcatag acaagcgttt gaagaggaaa
     1801 taaacacaaa caagaaggcg gatgagcgtt atttccgctt ttttgtttgt atttttctcg
     1861 tgaatactta tgaaatagta aatttttatg aaattctact ctaagcatca gactttcttt
     1921 tcaaaatatg ctattttaaa aatgatgaaa aaagggggag aaaaatgaat tttttagttg
     1981 ttttaaaaga tactctgatt gagcgattta catttctata tgttcaattg caggaaaata
     2041 aaacattgct gcatttttca ccagagttcc atctagaggg gctaacatta ggagaaatct
     2101 atcaagcaga agggctgtcg gctgttttgc acttttttaa ggtagaattg gaattaccag
     2161 taaaagatta tatcgagctt tctcttgaat cttgggatcg agcagtggtt gaactgtttc
     2221 cagaaggttt aatgatcgat acaaacgatg ggaagatcca tctgactgca gcagaactgc
     2281 aaaaacaaat gcgttatcaa atagatgacg aaaattcttt ttctattttt agacgtcagc
     2341 aaaagattct aaaaagcttt ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga
     2401 cacagctgtt gaaaaaatat ccaggtctgc ttcacacgtc tattcaattt cctcaattga
     2461 ttacgatgat ccatcgtttt atacgtatgc aacagctacc atctaagaag ctgtttcttc
     2521 cattgacgga cacatttcga gtagtcaaca gagaagataa aaagaaagtg attgtgattg
     2581 actttaccag aaatcggaat gtccttcacc aagttgcaat ggaaaaggcc aattaaggct
     2641 ttttcttttt ggattttttt gtcataataa atattagatg atggaggaga taatatgatg
     2701 caaacatggt tagaaaggct aatacgattt cagcagaact atcacaaact tatgcaggga
     2761 agatatgcga aatttgatca gttgaatcgc ggtctattga tcgctgcgct tgtctttagt
     2821 ttgctaagtc gttggttgcc ctatagaatc ggacaattgt tgttcatcgt ctttttcgga
     2881 tggatgatat ttcgtttcct atcaaaaaaa atctacccca gactaaatga gaatcaacgt
     2941 tatttgaaat atcttgatag agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa
     3001 gtcaaaatgg atcgaaaaag ttatactttt tttgagtgcc cgaattgtca tcaaaagcag
     3061 cgtgcaccta aaggaaaagg acgaatacgt gtgacatgca aaacatgtgg tataaaattc
     3121 gaaacgaatg tttaagggag aaaagaatga aattagtatt attacgtcat ggcgaaagcg
     3181 aagcaaattt tgaaaattac tggacaggat ggctagatgt cgccttaaca gaaaaaggaa
     3241 aagaacaagc agcagttgcc ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg
     3301 tttatacctc tgttttgaaa cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg
     3361 gtcagttatg gcttcctata ataaaaacat ggcgtctaaa tgaacggcat tatggggatc
     3421 ttgtgggtaa aaacaaagat aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat
     3481 ggcgcagagg atatacggaa gttccgccgt taaccgcaga aaatagattt gaccgcagat
     3541 atgatcactt ggatccgcat ttgatcccat acggagaaag tctggaaatg acagtgaatc
     3601 ggatcattcc cttatggcaa gatcatctag caccaaaact aaaagatcgg caagatttac
     3661 tagttgtggg tcatggtaat agcatccgag cactgacaaa atatttagaa gatgttcctg
     3721 aagatcaaat ggatacgatc gatatcccaa atgcacaacc aatccagtat acattagcag
     3781 atgatttgac aattctggac aaaaaaatac tttaaagaac acctttatct aaaagtgaga
     3841 gatagaaaaa agaagcaaag gaatggaaca aaaggaacat gaaaaattat atatgggact
     3901 ttgatggtac attgtttgat acgtatcccg caatggtaga tggtgcatgg caagcactaa
     3961 aagattttgg tatcagtatg gacaaaaaag aaatctactt caaaatgaaa aagtattcta
     4021 ctagttattt gatcaatgaa tcaaacttga atgccagaga gtttaatgaa ttatttcacc
     4081 gatatgaaaa agaaagtacg gaagtttccc gtccatttcc agaaacgaaa caagtactgg
     4141 aaatgctaaa agacaacggc ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa
     4201 catggggcct gttaaaagaa catcgattgg cacatctgat tgaggaagta gtagggatcg
     4261 atcaggactt tcctcgtaaa cctgatccag catctttaaa ttatttgatc gatacttttc
     4321 atttagaaag aacagacacg atgatgatcg gcgatcgtcg tttagatatc gaagcaggga
     4381 aaaatgcagg agtcgctact tgtctttacg acatcgatca ttttctgggt gagatcccgg
     4441 cagattatgt ggtaggaaat ctgaatgaaa tccttacctt gcagagcatc attaaaaagt
     4501 agtatcccct ttcaaaggag atatgttata attttcataa aggagtgata aaattatatg
     4561 gaaatcagaa gacaaaaaga agttgtagaa gcatttcatt atgatatgag attgccgaac
     4621 caagaagtag aaacagattt gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac
     4681 tatccgaaag aacatagcat cgtaggagca cgcttagagt tccgtttagt gtttgataca
     4741 tatgttttat ctggaagtgt gagtcagatc aatcatatcc tcaatcggaa aattgaaggt
     4801 caaaatgatg tcacacaaga agaagtggac gagctagtag caccgttatt cgatatcgta
     4861 cgccgaatgt cttatgaggt gacagagatc gccttagatc agcctggggt aaaattgaat
     4921 tttcaatcag aaaaacaaga atagacagta aaaacatacc tgcgatagat taaagctatc
     4981 acaggtatgt ttttttatta ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg
     5041 tctaaaatat aaaagtcact gaaaaaaatt ttttcaggag gatggttgaa gaatcattct
     5101 cgggaacctt tacttccttc gatggacttg tcattaataa tatgaggagg agaaaaatga
     5161 caacactcat cacagtttta gtggttctag tttttgcggc attattatat gtgttctatc
     5221 gtatgcaaaa gcgccataca aaattttcaa cacgcgtctt tacagcatta ggagttggta
     5281 tcgtctttgg cggtctgctt caattgatct ttggtgtaga cgacaaagtc gttactcaat
     5341 caatggaatg gatcgggatt gtaggtcaag gatatgtatc attcttgcaa atgctagtta
     5401 tgcctttagt gtttatttcg atcattggtg cattcacaaa aatgaaagtt tctgaaaagc
     5461 tagggaaaat tagtgcgact gtattgactt ctttacttgg aacaacagct attgcagcat
     5521 tgatcggaat tggttcaaca atgctgttcg gactccaagg agctaaattc acacaaggag
     5581 ctgcagaaac agcacgaatc tctgaactgg caacacgtca ggaaacagtt gaaaacttat
     5641 cgattccaca gcagatttta tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta
     5701 gtcgtgcaac aagtacgatt ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc
     5761 taggcgtgaa aagaaaagcg ccaaaagaag cagcattctt ttcagattta gtcgacagtc
     5821 tgtataaaat cattatgcgg atcgtgacgc ttgttttgcg tttgacacct tatggagtat
     5881 ttgctttgat gacaaacgtt ttggcaacaa gtgattttgc agctatcgtc aatttaggta
     5941 aatttgtcgt tgcttcttac gtagcattgc ttgctgtttt gattgtccac tcactgattt
     6001 tgattggtgc aaaagtcaat ccaatcaact atttcaaaaa agcctttccg gtattgagct
     6061 ttgcgtttac ttcacgttca agtgctggag ctttgccatt gaatatcgag actcagacaa
     6121 aagctttagg agtagatgat gcaacagcta atttctcagc aagttttggt ttgtctattg
     6181 gacaaaacgg ctgtgctggc gtttatccag ctatgttggc aacgatcgtc gcaccagcag
     6241 ctggagtgaa tgtctttagt ttggaattta ttttgatgct tgtggcaatt gtaacaatca
     6301 gttcttttgg tgtagctgga gtcggtgggg gcgcaacatt tgcttcactg atcgttttag
     6361 gggcaatgaa cttgccagta gcaatcgttg ggttagttat ctctgttgaa ccattaatcg
     6421 atatggcacg tacagcagta aacgtcagcg gtagcatggt agctggtatc gtgacaagtg
     6481 cacgcatcaa agatttagat cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga
     6541 atgcatagtc aaaaagaggt tgtgatagaa gtggtcagct ttaagaaata agaagaagtt
     6601 tcctaaaaat agtttctcaa atttttgtga aatttcggct tatttccgag aagctacttc
     6661 tgaaacaccg tgaataagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc
     6721 caaacaaaaa tagttcctca tattttacgt ttggttgagc ttattaccgc agatgcaagc
     6781 ttttgaacac cgtttaagaa agaggtcgtg agcctgccgt ttcactctga gtcacaaaga
     6841 gcaatttcaa aaaatagctt ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt
     6901 gtcaggagaa caccgtttac cagaggttgt gacttgtttt tagtcacgac ctcttttttt
     6961 atttattcca tataaataca cattatttat tccgaaaagt gaaagcgcaa ataacaaaaa
     7021 caaccaactt atttattatt aaagaaaagc gtatttaagt aggacgattc tgttgagagc
     7081 tttcgtagat tttcccattt tcctatggta aactgacaaa gttgagaatg ttagaacaga
     7141 t
//
LOCUS       DAZBTF010000108         6886 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000108 DAZBTF010000000
VERSION     DAZBTF010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.113"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACHGNS_002656"
     CDS             complement(<1..182)
                     /locus_tag="ACHGNS_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFN6938547.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACHGNS_002657"
     CDS             517..951
                     /locus_tag="ACHGNS_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFN6938548.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACHGNS_002658"
     CDS             974..1294
                     /locus_tag="ACHGNS_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938549.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACHGNS_002659"
     CDS             1435..1626
                     /locus_tag="ACHGNS_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938550.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ACHGNS_002660"
     CDS             complement(1697..1954)
                     /locus_tag="ACHGNS_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFN6938551.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="ACHGNS_002661"
     CDS             2112..2375
                     /locus_tag="ACHGNS_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938552.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ACHGNS_002662"
     CDS             2399..2587
                     /locus_tag="ACHGNS_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938553.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="ACHGNS_002663"
     CDS             complement(2670..2855)
                     /locus_tag="ACHGNS_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938554.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="ACHGNS_002664"
     CDS             3070..3441
                     /locus_tag="ACHGNS_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFN6938555.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="ACHGNS_002665"
     tRNA            complement(3591..3663)
                     /locus_tag="ACHGNS_002665"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="ACHGNS_002666"
     CDS             complement(3709..4137)
                     /locus_tag="ACHGNS_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938556.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="ACHGNS_002667"
     CDS             complement(4197..4955)
                     /locus_tag="ACHGNS_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFN6938557.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            5250..5447
                     /locus_tag="ACHGNS_002668"
     CDS             5250..5447
                     /locus_tag="ACHGNS_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938558.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            5553..6035
                     /locus_tag="ACHGNS_002669"
     CDS             5553..6035
                     /locus_tag="ACHGNS_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938559.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(6093..6689)
                     /locus_tag="ACHGNS_002670"
     CDS             complement(6093..6689)
                     /locus_tag="ACHGNS_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFN6938560.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
BASE COUNT         2331 a         1058 c         1353 g         2144 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     5041 gaaataatta gtttttaagg aatatgtttt aattttttga aaaaaattct tctaaataca
     5101 acagctgttt taattgtgaa aacgaaaaca gtacgctata atgcctgtaa ggttgatttt
     5161 taactgtaaa taaaaatcaa tgagttaaat ctccaaagaa atagcagtgg taatgtggag
     5221 aaaagttcca gcagtttagg aggattttga tgacggaaga aatgattaat ttaggagaac
     5281 aatatgcatg caaaccaatt ggttttacaa aaacggtcat tggtgaagtt gtatcaaaaa
     5341 tgacaaactg cgccgtggtc aaagttgccc aatgtgctgc agaggaccaa gaattattag
     5401 atgaaaaagc aagcatggtt gttgcaaaat atgatacatt tgaataaaga aaacaattgg
     5461 acgtgcggcg tgctgcaagt ccaatttctt tgagaaggta aatcagtatt cttttcctat
     5521 tttctggaga agaaaaagcg aggcgagttg tcatggtaac attctttacg gttctattgt
     5581 ttttattcgt tgttgtgatg tttttatttc tttggctggt tcgaaaagaa attatttatc
     5641 ggactgtgcg aaatcgatgg gtctacttgg tgataccctt cttagtgtca ctagtcattt
     5701 ggtatacact catcagtcat cctacagcag atgaattggc aaaagggatc ttgtcagcaa
     5761 tgatttttat tagttttttg ttggacagta gaggaatcac agaagaagga cttgttttga
     5821 atagctttga caaaaaaggt gttccttttt ctgagatcaa taaaattgta ttatatcagc
     5881 caaaaggttc taaaatcgta aaaatgaatt ttttccgaaa cggatggcga ggaccgatgc
     5941 tgaaattttc tgcatcatta gaagaattag ttccattttt gtcccaacgc ttaaacgatg
     6001 aggcagagat tgatattatg attgatccag aataaaaaca gaggttgcga caagatgttt
     6061 gtcacaacct ctgtttttta tttcaaataa aattatccaa atacgcggaa gcgatcggca
     6121 tcgttcatgt attctttttc ctcagccggt gtttctggtg aaccgaatac taattgcgaa
     6181 cgtagtttcc agtttgaagg gatattccat tcttttgcaa ctgcttcgtc aataactggg
     6241 ttgtaatgtt gcaagtttgc gcccaatcct ttatcaacta atgctaccca agtattagct
     6301 gttgcgattc cgtttgattg ttctgaccaa tctgggaagt tgtctgcata taattcaaat
     6361 tgttcttgta gaccttttac cacgtcagta tctttgaaga ataatactgt accataacca
     6421 tttttgaaac cagctaattt gttttgtgta tttgggaaag cttctgctgg agtcaatggg
     6481 cgaagtgctt cttcagtgat ttcccaaagt ttttcatgtg catctccgaa aaggataact
     6541 gcgcgtgttg attgagcatt gaaagcagtt gggctttctt taattgcttc tttgatcaaa
     6601 gttgttaatt cttcattaga aagtgaaaca ttacgtccta gatgatagat tgaacggcgg
     6661 tttttcaaag tgtcagtaaa agttgtcata ataaaatttc tcctttgatt tgcgttttgc
     6721 atttttttaa ttgattacga aatgaagtat agcaccttac aaaaagtaag taaacagata
     6781 tgctcacaaa aaataagcga agttcaaaaa acaattttaa taagaaaaat cgttatgtaa
     6841 ggattttctt agttatcgct ttcattgttg tgttttagga attttt
//
LOCUS       DAZBTF010000109         6824 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000109 DAZBTF010000000
VERSION     DAZBTF010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.114"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(75..278)
                     /locus_tag="ACHGNS_002671"
                     /pseudo
     CDS             complement(75..278)
                     /locus_tag="ACHGNS_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            244..441
                     /locus_tag="ACHGNS_002672"
     CDS             244..441
                     /locus_tag="ACHGNS_002672"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938561.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            complement(484..1059)
                     /locus_tag="ACHGNS_002673"
     CDS             complement(484..1059)
                     /locus_tag="ACHGNS_002673"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFN6938562.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1214..1740)
                     /locus_tag="ACHGNS_002674"
                     /pseudo
     CDS             complement(1214..1740)
                     /locus_tag="ACHGNS_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2197..3708
                     /gene="gadC"
                     /locus_tag="ACHGNS_002675"
     CDS             2197..3708
                     /gene="gadC"
                     /locus_tag="ACHGNS_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HFN6938563.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            3721..5121
                     /locus_tag="ACHGNS_002676"
     CDS             3721..5121
                     /locus_tag="ACHGNS_002676"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFN6938564.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(5286..6721)
                     /locus_tag="ACHGNS_002677"
                     /pseudo
     CDS             complement(5286..6721)
                     /locus_tag="ACHGNS_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT         2114 a         1172 c         1227 g         2311 t
ORIGIN      
        1 aatcttttta ccactccaaa cagggagcgt caataatttt gtgtaaataa ctcgtccttc
       61 tgcaaaataa tgggttactc agtaaacatt gaagctaatg tatccgtcac ttgttggaag
      121 cctttatggc ttctatttaa gaatttttga ttgtatgtat caaagatgga tactaggaaa
      181 cgctctagtg attcttcatt ttgaaactgc tcttttctgc ggctgtattt cttgatttgt
      241 ttattgaaag attcgattgt agaaccagaa ggaatggaga acacttttga actttacaac
      301 aaaactactg ttgataaatt aattccaatt ttagttccaa ctgatttaaa tggtcatgat
      361 gttttacttc gttttaacgg atttttaggt gaggattata gatacctaaa aatttctgat
      421 cctcaacctt taacagaata ataaaaaacg tgtaatcaat tatttgatta cacgtttttt
      481 atttcaatct tcgttgtagt agtggttgcg acataccgaa aggtaatctt cattacctcc
      541 tactacaatc tgttcacctg aataaactgg aataccatca acaatacgag cattcattac
      601 agccttttta ttgcaaaaat ggcaaatagt tttaatttcc tcaattttat cagcaaatgt
      661 caataatgct tcggatccct tgaagagatt attttgaaaa tcattcttca atccatagca
      721 aattactggt acttcctcat tcattacgat aagtgataga tcaataatat tctctctact
      781 taaaaattga gactcatcga ccaaaataca atcaactttt ccgtactgat caataatctc
      841 cactaattct ccgtacacat ccatttcaga agtaattgca atagctggtt tcgaaacacc
      901 tatacgactc tcaacttttc caaatcctga tcgagtatct attgcgcttg tcattatgat
      961 aactctcttc ccttgttctt cataattatg agccactttc agtagctcaa tactctttcc
     1021 actattcatt gttccatatc tgtaaaataa ttgtgccatt aaatttattc ccttctttta
     1081 caattcaatt attccttaat tccatctgct tttatgcagc tgctcgttta ttccttgata
     1141 tgtctataac atctactatc cctaaaacca atttataagc cattagagat ggtattcctg
     1201 aacatctctc atattgtttt ttgagtggta tatcatctga aaaactgaag gaatcatgaa
     1261 aatagcgaca tacttatcat ttagagttct aactttaaat gcatttactt cttcagtcag
     1321 cactttagtc atgttggaca tggtttgggg cgtgtagtga tgcccataca ttttctcaat
     1381 caagtctgcg atttccgaca tggtaatacc ttttcggaag aggtgaatga cggtctcctc
     1441 taatgtgtca ttcgtccgtc tataagcagg aacagtctgt tgcttgaact cgccattgcg
     1501 gtcgcgcgga atctggagat gaagttcccc atactcggtc ttgaccgtac ggtcatagga
     1561 gccgttacga gaattacccg tgttaaaacc aatgcgatcg tacttttcgt aatcgaggaa
     1621 agccgttaac tccgttttga gaagcgtatt gacagctttt tccaaatgga cacggaataa
     1681 tccattcaaa tcgcctttgt tcgctagagt ctttaggatt tctgtagtaa aatcgttcat
     1741 atggaagtcc tcttttctgt gaatgtgttg tggtaactct attctacaga agggacttcc
     1801 ttttttctat ttacacaaaa tattttacac tctcaacaaa agtatcttcg attaatactt
     1861 gctcaagtta tgtgcagcta cgaaaaagta ggtaccgagg tactttcttt ttcgcagctt
     1921 tttttggact tatgagaagt acattcgctg gaagaatacg atataaaacg agctttttca
     1981 ctaaagatat taatagagtg aaagaaacaa gcttttgttg tcagtttttt cacttttcta
     2041 aaaatgaaaa taaaaaattc tggaagtgga gaagaatttt gcttgctata ctttgtcttg
     2101 tagaaaagag gtagacaaaa taatgcaagc tcaaataagc gattttccat aaccttggta
     2161 ggaatgacaa attaaatagg aaagggaagt aataatatga ataaaaaaaa gttatctcta
     2221 ttcggttttt ttgccctgac agcttccatg gttttgactg tttatgagta tccgaccttt
     2281 gcaacatcac aattacattt ggttttcttt cttcttttgg gaggtatttt atggtttcta
     2341 ccagttgctt tatgtgctgc agaaatggca acagttgaag gatggaaaga tggtgggatt
     2401 ttcagttggg ttagtcaaac tttaggagaa cgttttggtt ttgcagctat ttttttccaa
     2461 tggtttcaaa ttacagtagg ttttattaca atgatctact tcatactagg agcgctgtcg
     2521 tatgtaatta attttccaga gcttaataca gatccattta tcaagtttat tggtgtatta
     2581 gtcattttct gggtactgac tttttcacag ttgggcggga ccaatcgtac cgctaaaatt
     2641 gctaaagcag gatttgtcat tggaattgtt attccttcga tcattctctt tggtttagca
     2701 gcagcctact ttatcggcgg taatccgatt caaatggcta ttaatgcgaa agcttttatt
     2761 cctgactttt ctcagctttc aactttagta gtatttgtat cttttatatt ggcttatatg
     2821 ggcgtagaag cttcagcatc acatattaat gagctggaaa atccccaaag aaactaccca
     2881 ttggcaatgt ttttactagt gattttggcc atcgccttag atgcaatcgg cggtttctca
     2941 gtagcagcgg ttattccaca acatgacttg tctctcagtg ctggtgtaat ccaaacattt
     3001 gaaacgctta ttttacattt taacagtcat ttacattggt tggtaaaagt gattgctctc
     3061 atgattgcct gtggcgttat gggtgaagtt agttcttggg tagttggtcc atcacgaggg
     3121 atgtttgcag ctgctcagcg aggtctgtta ccagttttct tccggaaaac taataaacaa
     3181 gacgttccag ttccgttgat tatgatacaa ggaattatcg ttactatttg ggcagcagtc
     3241 ttaacttttg gtggaggagg caacaattta tcattcttag tagcaatctc cttaactgtt
     3301 gtcatttatc tcgtaggata tttactattt ttcctaggtt actttgtatt agtttttaag
     3361 aaaaaagact taaaacgaac ttacaatgta ccaggaaaaa ctgttggcaa aacgattatc
     3421 ggtggaattg gttttatttt atcagtgttc gcattgctta tctcttttgt accaccagct
     3481 tcaattgcag ctagtgagca ccgaacatac caaatcatac tacttattag ctttgtagtg
     3541 actgttcttt taccttttat catttatgaa gtacatgata aaagtgctca cacaactatt
     3601 gaggaaccta ctcacgtgaa agctgaagac gttaatcccg ctgtttatcc agctgctaga
     3661 ggagagcacc acgttacaaa aaatgatgaa catattttaa aacattagga ggaattcatt
     3721 atgttatacg gaaaagataa tcaagaagaa aaaaactatt tggaaccaat ttttggctct
     3781 gcaagtgagg atgttgactt gcctaaatat aagttaaaca aagaatccat tgaaccacga
     3841 attgcttatc aattagtaca agacgagatg ttggatgaag gaaatgcgcg attaaactta
     3901 gctacttttt gtcaaacgta tatggaacct gaagcagtga aattgatgac ccaaacgtta
     3961 gaaaaaaatg caattgataa atcagaatac ccacgaacaa cggaaattga aaaccgctgt
     4021 gtaaatatga ttgctgattt atggcatgct ccaaataatg aaaaattcat gggaacttca
     4081 acgatcggct cttcagaagc ctgcatgctg ggtggtatgg ccatgaaatt tgcttggcgt
     4141 aaacgtgctg aaaaattagg tcttgatatt caagcaaaaa aacctaacct ggtgatctct
     4201 tctggttacc aagtttgttg ggaaaaattc tgtgtatatt gggatgtgga actgagagaa
     4261 gtcccaatgg atgaaaaaca tatgtcaatt aatctagata ctgtcatgga ttatgtggat
     4321 gagtacacaa ttggtattgt aggtattatg ggtattactt acactggtcg ttatgatgat
     4381 atcaagggtc tgaatgattt agttgaagct cacaataaac aaactgacta taaagtatac
     4441 attcatgttg acgctgcatc gggtggcttt tatgcaccat ttactgaacc tgatctagtt
     4501 tgggattttc aattgaaaaa tgttatctca attaattctt caggtcacaa atatggtttg
     4561 gtatatccag gtgtgggttg ggtcttatgg cgtgaccaac aatacttacc agaagaatta
     4621 gtatttaaag ttagttactt aggtggagaa atgccaacta tggctatcaa cttctctcat
     4681 agtgcagcac aactaattgg gcaatactac aactttgtac gctatggctt tgatggttat
     4741 cgtgatattc accaaagaac tcatgatgtt gctgtttatt tagccaaaga gatcgaaaaa
     4801 actggtattt ttgaaatcat taatgatgga tcagaattgc cagttgtgtg ctataagctg
     4861 aaagaagatc ccaatcgcga atggacacta tatgatttat ctgatcgtct gttaatgaag
     4921 ggatggcaag tcccagccta cccattacct aaagacttgg atcaattaat tattcaacgc
     4981 ttagttgttc gagcagactt tggaatgaac atggctggtg attatgtaca agatatgaac
     5041 caagcaattg aagagttgaa taaagctcat attgtttatc ataaaaaaca ggatgtgaaa
     5101 acgtacggct ttactcacta aaattcttca gagaaatata aaagaagtta attaactaat
     5161 aaaaggagtt gtgattacca cacagctcct taaatctatt tgaataaata tatgatattc
     5221 atttgttaca ctctcaatct tagctgttgt tctattcatc attactctta ttgtaagtag
     5281 ttcagtcaaa tttgcttgtt caattgatcg ataacagtat gaattgtttg aaaagtaatc
     5341 ggatatataa ttgttttctc actacctaag ccttcttggt ataaagaact atcgcaaagt
     5401 ataatccgct gagtacaaaa gaaagtgtcg tctactattt ctctaagttg tttacaaatg
     5461 atattactaa tagaaaagtg ttctcccaaa atgtcttcta actgttgtcg atagtataaa
     5521 tacctaaagt ctgacggagc tactatgaac acttcaattg gagatttccg tttatgctca
     5581 tttaaaagac ttaaactaat ggttagttta cgaatagaat tctcattcca acgcagaaat
     5641 aaattgtttt gttttcgcca ttgttcaaga atttcatgaa cttctaagta caaagcgcgt
     5701 tgctcttcat ttaacaagta agtcttatca ggttgaaata tctgaatatc agcccaaagt
     5761 gagcggataa acgggagaag tgctttatca aaaagtatct gattttcttc agatatattc
     5821 agagataaga taagtttttg actgagctca atcaccaaag ggttatctgc tacatggttt
     5881 tgataaacaa cgttcaagtc tttttgtaac aattcaaagt tatcgcatag ataatttcta
     5941 gagttgaata gaaagtagat ataaaggcat tcatcttcgt catgacataa agagaaagaa
     6001 agttgctcta atcctttctg tattagctga aacaagggag tatgtcgggc aactcttttt
     6061 tcttcctgac taaaatatat tgggttagtc ttagatctct gcaaaccgat agcgatacct
     6121 aataaaataa attgtttttc aactgtaggg taacttcttg aaaaaaatgt ttttctacat
     6181 aatcaatcaa ttgttgaccg ctttgaataa ttgatttatt tagctggatc aactctgtcc
     6241 gatttgtctc atagattatc gttagtaaca aacttcgata atccttttca ggaatttcaa
     6301 aatggccatc agcatttaag tactgcattt gtttaaaaaa attaatcaac tcatttcgta
     6361 agttgtaaac tttgcttaac gaaatgaagg tagtatcagc tatattactc caattgtgtt
     6421 cccctgataa ataatgtcct agtacattga aaaagtcaga gcgctgatag atgagttgag
     6481 tgagtgccaa cctcgaaaac gaagagtcga atgcgactgt aaagctactt ctagatttct
     6541 ccactgaaat aatttgctga tggagtatgt cggtaatgca ttccaagtcg ttagtgacag
     6601 tgagtgggca aacgttcaat tgatctgcta tttcgcgaat ctgaatggtc cctttttcgt
     6661 gtaattgctc acaaatgtaa acttgacgaa aaatactttt ctccagatag gtttccaaca
     6721 tagtactcct cctagtcatt ttatacataa ctattataac tctaccaatc ggaatagtcc
     6781 tgtaatatat tgaatcaaaa atataaatta taaaataatt agat
//
LOCUS       DAZBTF010000110         6799 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000110 DAZBTF010000000
VERSION     DAZBTF010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.115"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(208..666)
                     /locus_tag="ACHGNS_002678"
     CDS             complement(208..666)
                     /locus_tag="ACHGNS_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFN6938565.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(679..2010)
                     /locus_tag="ACHGNS_002679"
     CDS             complement(679..2010)
                     /locus_tag="ACHGNS_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFN6938566.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(2021..3463)
                     /locus_tag="ACHGNS_002680"
     CDS             complement(2021..3463)
                     /locus_tag="ACHGNS_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN6938567.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3450..4214)
                     /locus_tag="ACHGNS_002681"
     CDS             complement(3450..4214)
                     /locus_tag="ACHGNS_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFN6938568.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4318..5193)
                     /locus_tag="ACHGNS_002682"
     CDS             complement(4318..5193)
                     /locus_tag="ACHGNS_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFN6938569.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5448..5532)
                     /locus_tag="ACHGNS_002683"
     tRNA            complement(5448..5532)
                     /locus_tag="ACHGNS_002683"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5496..5498),aa:Leu,seq:caa)
     gene            complement(5550..5621)
                     /locus_tag="ACHGNS_002684"
     tRNA            complement(5550..5621)
                     /locus_tag="ACHGNS_002684"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5587..5589),aa:Gln,seq:ttg)
     gene            complement(5631..5703)
                     /locus_tag="ACHGNS_002685"
     tRNA            complement(5631..5703)
                     /locus_tag="ACHGNS_002685"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5668..5670),aa:His,seq:gtg)
     gene            complement(5765..5849)
                     /locus_tag="ACHGNS_002686"
     tRNA            complement(5765..5849)
                     /locus_tag="ACHGNS_002686"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5813..5815),aa:Leu,seq:caa)
     gene            complement(5870..5940)
                     /locus_tag="ACHGNS_002687"
     tRNA            complement(5870..5940)
                     /locus_tag="ACHGNS_002687"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5906..5908),aa:Cys,seq:gca)
     gene            complement(5986..6057)
                     /locus_tag="ACHGNS_002688"
     tRNA            complement(5986..6057)
                     /locus_tag="ACHGNS_002688"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6023..6025),aa:Gln,seq:ttg)
     gene            complement(6069..6141)
                     /locus_tag="ACHGNS_002689"
     tRNA            complement(6069..6141)
                     /locus_tag="ACHGNS_002689"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6106..6108),aa:His,seq:gtg)
     gene            complement(6144..6217)
                     /locus_tag="ACHGNS_002690"
     tRNA            complement(6144..6217)
                     /locus_tag="ACHGNS_002690"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6183..6185),aa:Trp,seq:cca)
     gene            complement(6224..6304)
                     /locus_tag="ACHGNS_002691"
     tRNA            complement(6224..6304)
                     /locus_tag="ACHGNS_002691"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6268..6270),aa:Tyr,seq:gta)
     gene            complement(6306..6381)
                     /locus_tag="ACHGNS_002692"
     tRNA            complement(6306..6381)
                     /locus_tag="ACHGNS_002692"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6346..6348),aa:Phe,seq:gaa)
     gene            complement(6417..6489)
                     /locus_tag="ACHGNS_002693"
     tRNA            complement(6417..6489)
                     /locus_tag="ACHGNS_002693"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6454..6456),aa:Asp,seq:gtc)
     gene            complement(6499..6571)
                     /locus_tag="ACHGNS_002694"
     tRNA            complement(6499..6571)
                     /locus_tag="ACHGNS_002694"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6536..6538),aa:Val,seq:tac)
     gene            complement(6587..6658)
                     /locus_tag="ACHGNS_002695"
     tRNA            complement(6587..6658)
                     /locus_tag="ACHGNS_002695"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6623..6625),aa:Glu,seq:ttc)
     gene            complement(6673..6762)
                     /locus_tag="ACHGNS_002696"
     tRNA            complement(6673..6762)
                     /locus_tag="ACHGNS_002696"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6726..6728),aa:Ser,seq:gga)
BASE COUNT         2065 a         1486 c         1095 g         2153 t
ORIGIN      
        1 aattttattt caatatattc tcttaaataa tcaaatttat ttttaagaat cctgaaaata
       61 agttttctaa atcataggag caacaaaaaa attcgataat taacaaaaat caaaatgatt
      121 aaatctagtg attgaaacat atcatttcaa aaaaattttg taaattcacc cataaatatc
      181 accctattta acttttttca tcgtacacta aaattcaaaa caactatctt taatctttac
      241 ttcttggttt tgttttaata aatttccttc aaaaatgcct ggagaaacta caatgatcac
      301 cgtatctttt tgtgcttgct ccaacttctt caaatctata atagctaaca gtgtcccttc
      361 ttttacctcc tggttacttt caacccgtat ctgaaacgga cttccttcga gttcaactgt
      421 atctaaaccc atatggatca gtatttttct gccagattca ctttcgatcg ttatggcatg
      481 tcgggtagga aatatattat cgattattcc tttaactggc gcatggatat atccatcatg
      541 attcgttata gcgtatccaa ctcctatcat ttttgaagaa aagacctcgt cttttactgc
      601 tgttaatgga ataatttgac cactagctac agcttttaga atgtttcttg ttttaaagaa
      661 atgcactttt tatcacctct actttaatgc tggccaatat tttttgttta ctttgatcat
      721 ttcatctagt attttttttg cgactctggc atttggtacg gtttgattca atgtaaatgc
      781 ttgtaaagct ttttgataag aaccttcaaa gtaagcatct actagtaact tctctactgc
      841 aacttgtgcc tccatcaatc ctttatgaaa atcactgatt ggttttctta gtgtaattgg
      901 ctccacacca gttgcaccaa catatgccgg tatttcaaca actgcatctg accgtagatt
      961 cggaatcgcc ccttgattag gaacgattag cataaagcga cgacgatcat cattcaacaa
     1021 ggaaatagct atatcgacga tatattgtcc atgctcccca aaattaaagt tcaatacttc
     1081 ttccacaata ccgcggcgta ttttatcagc catttctttg gtatttttct ctcgtccatc
     1141 catgaccata ttagcacgtg tatactcttt gtcagtatgc tcaacgacca tatcaggata
     1201 taagtaatac tctaagtaat tattcgggat attacttgga aaatttttcg tgatgactga
     1261 catcatttga aaggtttttt gccaagtttt atctcctata ttaaaatcgg cgacttgcat
     1321 ttcttttgtt attagttttt caataatctc tggcataatt tctcgttgca attctttatc
     1381 ataaatcgat gtataccagc caaagtggtt taaaccataa tacgtaacga tccaattctt
     1441 acgatcataa ccgtaattca ctgcaatcgt ttcttcaatt gaaatagtca tatcacaggc
     1501 attaatcatt tttacattcg gatatttacg acgaacagct tcagaaacga tcgactctgg
     1561 atttgtataa ttcaaaatcc atgctttggg ggcatagtct tgtatatgat cgacaatttc
     1621 caataaacct ttgattgagc gcatcccata agcaaatcct ccgagtccac acgtttcttg
     1681 tcctactaat ccatgtttaa gtggaatctt ttcgtccttt tctcgcatct ccaatcctcc
     1741 aacacgaatt tgtgagaaaa caaaatcgca tcctgtaaaa gcaagctttg gatcatccgt
     1801 tgcacgaatg cgaatatttg aaaaaccttt tcttttcagc ataaatttaa caatcaagta
     1861 catatccata tttctttctt catcaatatc atataaacgt atttcagata atggcaaccg
     1921 tttttcattg ttcaaaatgg cttgaacgat tcctggtgta tacgtactcc cgcctccagc
     1981 tatagtgata atctgttttt tcatcatcat cttttccttt ctattcttct aataatttat
     2041 tgaattctgc cataacataa gtcacttcta agccgatgat aatttgataa tttttttcag
     2101 ttggtttaat gatcccgctt atccctaatt gcttgattcg ctgttcctcg accatggtgg
     2161 gatccactac ctctacccgt aggcgtgtat tacaactata gacatccagg atattggctt
     2221 ttcctccaaa cgcttgtagc atttttgttg ccatatattc ataattttta cttgctaacc
     2281 ctaactctgt ttcttctaag gttacttcat tgccaaactc tgccttagct tcacgtccta
     2341 atgtctgaat gtttctctta ttgatcaaat aataaaaagt aacgtaatac aaggcaaaaa
     2401 agaaaattcc gattggaaga atcaaaatag cattggtagc gattcgaaaa ttgatgagat
     2461 aatcaacaat acatgctccg aatgtaaaac caattcgtat attgaataag taacagacaa
     2521 tccctgctaa accggcaaaa agtgcatgga tcacgtacaa cataggagaa agaaacatga
     2581 aactaaattc aacaggctca gtgatatttg caataaatga agttcctgca ccgcttccca
     2641 tgattccttt gaccatctct ttattttctt tataagctgc tttatagata gctagacata
     2701 tagctggtat accaaacatc attactacaa aaaaaactga taaaaacagt ccagcctgag
     2761 gatctccgtt gataaagcgt gtcatctctc ctctgaaaac ttccttgcta ggagatgtat
     2821 agcttcctaa atcgtaataa atatacgtat tcaacacatg gtgcagtcca aaggggatca
     2881 gcaaacgatt caaaaacata aatatcccca cacctaatgc tcccatatca acgagcagtt
     2941 ttgcaaaatt atcaatccca atttggatga gcggccagat aaagcccaaa aaaacagctg
     3001 cgattgtttg tagaatcatc accatagtca atgggaattt ttcgcccgca aaataagaaa
     3061 aaactactgg taatttttgt tctttaaagc gattatacgt ccatgccgct actaaaccgg
     3121 aaataattcc accaaaaatc cccatattca cttctgggtc cataatttct aagccttttt
     3181 tcaaggtagc aatcgctaaa aaaccagtaa gtgcaggtat tcctttgtcc ttatttttga
     3241 caaaaccgat tgcaactcct atagcaaata agatatccaa gtttccaaaa acggcatttc
     3301 cagctgcacc aattactggg atgttcagca tatcgtcagc tgataaacgc atgatcaatc
     3361 ccgctaatgg catcgctgca actggaagta gcatcgctct tccaagcttc tgcacatttg
     3421 atttaacatt catttttatt cctcctctat taaatatcct catatgttac taactcaatc
     3481 aaaggatcat ttttcacttt ttgagcaaat gctgaatgaa ttaaaaattc gacttggtgg
     3541 gtacgaggat acacaaaact tgaaccattt aacaattcct gatccaaata cgctgcgtga
     3601 cacatgactt cgatcgtttt atcttctctc aatatcatga gtaaacttgc caaatattct
     3661 tccacactcg tattgatttg tcgttttttt tcatcagttt ctcctacgac atcgaaacaa
     3721 ggctcaaaat aatcgacatg acgaacttca tcttttcgtt caaagttgct ccgaaccggc
     3781 aaatcatatt tttttgctaa tgtaacaact acctcctgat tttcatccaa agtatgagta
     3841 tgatgatgac tgtctaaatg agttggtatg atccccgcat gataaatttt ttgaatctga
     3901 gcattccatt cctgatacag ttgatctttg tcaatgttaa atggatgctg ataataagat
     3961 aaggatctga actctccgct ttcgataaga gtatcgacag ttttgaggag tggtttgcca
     4021 cacgtcaatg ttaaatggac tcctacacct agcctaggca tttcctttct tagtttcaca
     4081 gcatgctcga aacccggcat atttgccatc aatgtagttg atgtcaaaat tcctcgctga
     4141 taagcatcca taatcccata attcacacca tggctatagc caaaatcatc cgaattaaaa
     4201 atcacttttc ccatttcatt ctccttcttt tttctactaa aataggcgca accaaataat
     4261 catagtccat ttactatgtg tcatttggtt gcgcctatag atagttacgc ccttatttta
     4321 atagattctt gctagatgaa ttgccaaata gatgatttca ttttcttgaa tggcaagttg
     4381 aaattgctgt tcgatgatat ctttaatctt tagtgctgta caatacgctt tgctatgttt
     4441 aaatgtttca gtaaaaaaac caatattttc tactggcgtt tcttcctgat aaatcaagcg
     4501 tttaaccagg aatttgatat gaaccattaa gcgatgataa gtcagtgatt ctttatcata
     4561 aacgtttgaa taggattttt ctagatggga caaaatcgtg gtcgtcattt ctactatttt
     4621 taagctcttt aaactattag gctctcctag ctcagcatta atgatatgca tagtgatgaa
     4681 tcctacttca tttgacccta actcggtttg atatcgatga ttgattcgct cgaccacttc
     4741 tactgctata tcatattcat ttggataaag gaatctcatt tctggcaaca taattgctgt
     4801 gacatctcta tcttctcttt ttcgttgaat agcaaaataa atatgatctg tcaatgtaat
     4861 atagatattt gaatctaatg gagaggagat cctagctgtt atcagttcaa tcacttcttc
     4921 cgcaaaatcc ataaattcga ttggaatctg cgagactatc tcactgaatc gtttgatctt
     4981 ttctttagaa tctaatacaa ataatttgtc agcatcctca acatcaaatg gcttactcac
     5041 tttagcagaa aagccaatcc ctttccccat cgcaattttt tctattccat cgtcatcgat
     5101 caatacagca ttatgattca gctttttcat cacatacacc gttaattcac ctcctctata
     5161 tgataaaaca aaacaacttg cagactcctt catacatatt tttgtctcca aacaattttt
     5221 aaagacaaaa aacgacctaa gaaagaaccc tttcttaggt cgtgcctaat tgaatagtag
     5281 caccctacat aatttacaaa aatagtttaa cacataataa aagcgttatc aatatctttt
     5341 ttactcgttt tatgataata cttgtatcga attgatttgg aggtaattac ataaaaacaa
     5401 aaaaaatata gcaactctct ataagaaagt tgctatattg ataattatac cggcggccgg
     5461 ggtcgaaccg gcacgtcctc aacggacact ggattttgag tccagcgcgt ctgccaattc
     5521 cgccacgccg gcaaatttaa aaataataac tggggtagct ggattcgaac caacgcatga
     5581 gggagtcaaa gtcccttgcc ttaccgcttg gctatacccc aagaataaag ggcgaatgat
     5641 gggaatcgaa cccacgaatg ccggagccac aatccggtgc gttaaccact tcgccacatt
     5701 cgccatcata aaagatgtta tttaatttct caataaaaaa ctaagatgaa gacatcttag
     5761 tcaataccgg cggccggggt cgaaccggca cgccctcaac gggcactgga ttttgagtcc
     5821 agcgcgtctg ccaattccgc cacgccggca agttgattta ttagtactaa ggcggtaacc
     5881 ggatttgaac cggtgatgaa ggttttgcag acctctgcct taccacttgg ctataccgcc
     5941 attatactat tatcaatcta taaaataaag aataaattat agaaactggg gtagctggat
     6001 tcgaaccaac gcatgaggga gtcaaagtcc cttgccttac cgcttggcta taccccaaga
     6061 ataaaaaggg cgaatgatgg gaatcgaacc cacgaatgcc ggagccacaa tccggtgcgt
     6121 taaccacttc gccacattcg ccatggcagg ggcagcagga attgaaccca cactaacggt
     6181 tttggagacc gctgttctac ctttaaacta tgcccctata aaatggagga gagtggattc
     6241 gaaccactga accctaagga acggatttac agtccgtcgc gtttagccac ttcgctactc
     6301 ctccatggtg gcgcgggaca gaatcgaact gccgacacac ggagcttcaa tccgttgctc
     6361 taccaactga gctaccgcgc catattttaa aattattaat tgaataacaa gaacgacggt
     6421 tccgacggga atcgaacccg cgatctcctg cgtgacaggc aggcatgtta acccctacac
     6481 cacggaacca gttcattatg gaggttaacg ggatcgaacc gctgaccctc tgcttgtaag
     6541 gcagatgctc tcccagctga gctaaacctc caacattcaa ttaaaatgac ccgtacggga
     6601 ttcgaacccg tgttaccgcc gtgaaagggc ggtgtcttaa ccgcttgacc aacgggccag
     6661 gtaaatttgc tacggagagt aagggattcg aacccttgag acagtttggc accatctaca
     6721 tgatttccaa tcatgctcct tcggcctctc ggacaactct ccagagtgaa agaagctcaa
     6781 tactctttct caaaatgag
//
LOCUS       DAZBTF010000111         6644 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000111 DAZBTF010000000
VERSION     DAZBTF010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.116"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..859)
                     /locus_tag="ACHGNS_002697"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ACHGNS_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ACHGNS_002698"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ACHGNS_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFN6938570.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ACHGNS_002699"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ACHGNS_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="ACHGNS_002700"
     CDS             complement(2143..2373)
                     /locus_tag="ACHGNS_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFN6938571.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="ACHGNS_002701"
     CDS             complement(2603..3421)
                     /locus_tag="ACHGNS_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFN6938572.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ACHGNS_002702"
     CDS             3582..4271
                     /locus_tag="ACHGNS_002702"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFN6938573.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ACHGNS_002703"
     CDS             4285..5787
                     /locus_tag="ACHGNS_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN6938574.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="ACHGNS_002704"
     CDS             5800..6276
                     /locus_tag="ACHGNS_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFN6938575.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="ACHGNS_002705"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="ACHGNS_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1204 g         2236 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DAZBTF010000112         6526 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000112 DAZBTF010000000
VERSION     DAZBTF010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.117"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACHGNS_002706"
                     /pseudo
     CDS             complement(99..1229)
                     /locus_tag="ACHGNS_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ACHGNS_002707"
     CDS             complement(1878..3080)
                     /locus_tag="ACHGNS_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN6938576.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ACHGNS_002708"
     CDS             complement(3140..3343)
                     /locus_tag="ACHGNS_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFN6938577.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4552)
                     /locus_tag="ACHGNS_002709"
     CDS             complement(3485..4552)
                     /locus_tag="ACHGNS_002709"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFN6938578.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4610..4717)
                     /locus_tag="ACHGNS_002710"
     CDS             complement(4610..4717)
                     /locus_tag="ACHGNS_002710"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6938579.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4880..5692
                     /locus_tag="ACHGNS_002711"
     CDS             4880..5692
                     /locus_tag="ACHGNS_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6938580.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6066..6257)
                     /locus_tag="ACHGNS_002712"
     CDS             complement(6066..6257)
                     /locus_tag="ACHGNS_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938581.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         1974 a         1323 c         1018 g         2211 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagctaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaa
//
LOCUS       DAZBTF010000113         6500 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000113 DAZBTF010000000
VERSION     DAZBTF010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.118"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            16..2691
                     /locus_tag="ACHGNS_002713"
     CDS             16..2691
                     /locus_tag="ACHGNS_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFN6938582.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            2873..3112
                     /locus_tag="ACHGNS_002714"
     CDS             2873..3112
                     /locus_tag="ACHGNS_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938583.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            3128..4501
                     /locus_tag="ACHGNS_002715"
     CDS             3128..4501
                     /locus_tag="ACHGNS_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN6938584.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            4505..6139
                     /locus_tag="ACHGNS_002716"
     CDS             4505..6139
                     /locus_tag="ACHGNS_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN6938585.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTSLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6302..>6500
                     /locus_tag="ACHGNS_002717"
     CDS             6302..>6500
                     /locus_tag="ACHGNS_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938586.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2277 a         1060 c         1307 g         1856 t
ORIGIN      
        1 agtgagtggt aattaatgaa aatgaatcac acttcaattc agaagcattt acaactgtta
       61 aatacactgg ggatttctta tgttaatttg agaacttttt ctacattaaa caagaaaacc
      121 gtttctcata ttttttctac tgcagattct caagcaatta ttaactattt agaaaaacag
      181 aaattgtctg aaggtacagt aaacataact tataatccta ttaaacaaga ggtgattaca
      241 caaaaaaagc attctgtgag agacaatgat attgaaaaaa ttcggtttgt atttattgat
      301 attgacccta aaaggaaaga gggaagtgca acagatagcg aaaagaaaaa ggcagaaaat
      361 gttatggaac aagtagaacg ttatttgaaa gaaaagagaa tagaatcatt tgtaaaagtt
      421 gatagtggga atggctacca tatttttctt cccataaatg aacaaccaaa taatcacgag
      481 acgattctta ctattcagaa ttttcttcaa ttacttcacc gaagatttgg agtagaagat
      541 gaagttgata ttgatacttc tgtttataat ccttcaagat tatgcaaatt agttggtaca
      601 ccagcggtca aaggaaagtc cacaaaagag cgtccgcatc gtttatctag tttcctttct
      661 attcccgata acatagatag aagtaattgt tttaaaattc ttgaacagat agttgaagaa
      721 aattccatga tgtcaacctc tagagtaaaa agccatccat cttttagaga agtgttacct
      781 tttattcaag cagactataa acaatggtta gctcactatc ctgaattaac ttataccata
      841 aaagagggcg atcataaagg agttacctta cttatatttg acgaatgtcc attaagaaaa
      901 cacacgaata attctaatgg ttcttgtttg tctgttacgg ctgataataa gattcgattt
      961 cattgtctgc atgcttcgga taaaaataaa gatattcaag actttgtaaa aaaatatccg
     1021 ttgccaaaac ctgtaaatcc ttctgtagaa tcattagaat ccaatcaaga atattatttt
     1081 gaccagttta aactgaatca agaaggagta tttttggtga aagaaggtca agaagaaaaa
     1141 gttgcttgtc cgatgtttat taagcaaata cgcaaaaata tcgaacaaaa agtcattaca
     1201 cttgttcttg cctattatgt cgatcattgt tgggatgaaa tagaaataga aggtggatgt
     1261 ttacaaacta ataattttaa acaattagca aaacatggct taatcattaa agctagaaga
     1321 gaagctgaag tcgttgactt tttacagatt caacgggaga atgctccaat cttttttgaa
     1381 catgaatctc ttggctggaa aaaagaaaat gaagcaatga tttttcagtt acaagattct
     1441 tttggacgtg aaattccttc tgtattaact aaaaactcat gctaccaatt aacaactaat
     1501 ggtacagaag cggaatttga cgcattggtt cataagtatg ttttaggaac gaacttagaa
     1561 cttgcttggt cgattggtat tgcttctatt gtgttaggct atttaaacgt tacgaaggct
     1621 aatacttttt tgagtttact aattaaccta cagggcaaga gcacaacggg taaaaccact
     1681 acgttacact ttattgcctc tttatttgga aatccaacta cggttcttag aaatatgaat
     1741 gctaccaata aagccattat aaagctagca tcaaacaatc agggaggtgt tccactaata
     1801 ctagatgagt taggagcagc aactcaaaat aactttagct cgttgatcta tcaattagct
     1861 tcaggagaag aaaggctaag attagattct aattgtcagc ttatgccaca agagactttt
     1921 tctgttttga cccttacctc ttctgaagag cgtttggaaa attatttcaa tgagaaatta
     1981 actggtttga aagttcgata tatcgaattt tctgatatca agtttacaaa ggatgcgaat
     2041 cacgcagaga aagttaaaag tctttcttct aatacttttg gttggggtat aaagttattt
     2101 cttttgaaac tctttgaaca agggattaca aaaattgaag caacttttgt cgaagctcaa
     2161 aaaaaactat gggctgtttt acctgaacat tctttgaagc aacgcattgg taataatcta
     2221 gctttgattt atagtggagc cctcctaatt caagatatac tgggctatcc tttacgatta
     2281 gagcatatac agacggtgct tattaattcg tacgaacagt taattgtaga tactagttta
     2341 ggaagtgagc aaacggtatt tgaccgagtg aaaacattgt tggtgatgaa tagccataaa
     2401 tttttaacaa acagtgacaa caaacatacg cccagtacga tttggggaaa agtatcaatt
     2461 caaaaaaatg gggatattaa ggtaaatatt tttcctatcc cgtttgagga cttgttaagg
     2521 cgagaatttc aagtaaagga tctaacctat ctttttaggc aattgcttga ttcgggtaat
     2581 atgtgtgcag aaaaagggag aagaacgaaa cgtattcaca tgaatcgtca agttcttcca
     2641 acatacgaga ttctattacc ttcttcactc aaatcgtatt ttcgattgta atttagaata
     2701 aaccaaagta tgaaagtgag gcaccatgtt atggaaatat ttcttattga tttagcaaaa
     2761 caactgatta cttatctgta tgatagcgag caaacccaaa cggaagtaaa tgaagcttga
     2821 tctatgcttc atttacttca ttcaatcaaa catgaaagaa aggaaaattc taatgactaa
     2881 aaataaaatg acactaaaag cagaggttct tctgtatatt caagaacatt tttcaaatca
     2941 agcctttttt acaaaaccaa tttacttgga tttcgaaata agaggggtat ctgctggatc
     3001 tatcgggggt actcttcaag cgttgaagaa cgaaggatat ttagaaaatc actttgtgca
     3061 acgttcgttc aatagaagag tcgtgaaaaa atggtattta gttcattcat agagagggga
     3121 atcaagcatg cgtgtggctt gttatgatcg aacgtctgtt tatgaagaag cgaaaattag
     3181 taatgacgct caatatgatg cagtgaaaga gtatgtttta gagaaatatg aacaaattcc
     3241 ccaacgattt tcagacaaag gagaatcagg ggaaaaagcg gagcgtccag cttttcgaca
     3301 gcttagagct tgggtttgtg cgtcagaaga agagggggtg atctatgttt ggcgttatga
     3361 tcgattggca cgtcaagcca aaatagcctt agaatttatt gagttgtgcc aacaacatca
     3421 ggttcaagtg tattcgattt ctgaacctct tcctgcttct ttctcacacg cgtcaacgaa
     3481 taaactattt gtgcaattat tgttcacttt tgcagaacaa caacgaaacg tcattattga
     3541 gaatgttcgt gccggacttt cctataaaaa gcaacaacgg caatatttgt cttctgcggt
     3601 accatatggg tatcgtttag tgaatgggaa aatccaacaa gaagtacacg aagcaaggat
     3661 tgttcgctat atttttcaac tttatttgac caaaaaatat ggctacaaaa agctttgtca
     3721 acgattgacg caacaaaagc ttttcttccg cgaacgtcct tttcagccct atcatattta
     3781 tagtatccta aaaaatccat tgtactatgg cgaaatcaaa ggaggatctt tggggaagta
     3841 tctaggaacc tttgaaccga ttctctcgaa agcaaccttt ttacaagccc aagagattcg
     3901 ccaatcaagg tgtacagcta aaaaagatac gtatctgtac ttattacgtc aaaaaattcg
     3961 gtgtcccttc tgtggtcgtc atctgagttc gaagtatcaa tggaatacaa agaagacgaa
     4021 gacgcttcat tattatcatt gtaccgaccg aagctgccaa ggaatttttc ttcgggcaga
     4081 aagggttgag tcagaagtat tggaaaagat acaggctttt tttcaatacg aaggcgtcta
     4141 tcaagccttg attgcccaat taacgcaaca attagccgaa aagcgaaaag atgaaaaacg
     4201 caaagtaatc caacaaaaga aacaagaagc acaatggatt aaggcatttg aagagggcaa
     4261 ttgttcactg gaagaactca agcaacaact gcaagctctt caaaagccga tgtctacttc
     4321 ttcgtttctt tcactccaac agtgtgaagc aaaagtgaaa gaatggcttc aactaaaaga
     4381 cctctctagt cgacaattat tatgtgacta tgtttgtcgg attgaggtaa caaaggataa
     4441 aacagtttgt gggatttact tccgacaact gacacaaaat attttagaaa ggaacgaatg
     4501 atctatggaa atgatacaaa ccaaacgagt cgcattgtat attcgcgtga gtacacaaga
     4561 acaagcttta gaaggacata gtattcaagc acaagaacaa aatggacgac agtttgccga
     4621 acggatgggc tatgaagtgg tagaagttta tgccgatgaa ggaatcagtg ggaaatccac
     4681 aaaaaatcgg ttcgcctatc aacgaatgat gaacgatgct cggaaccatc gttttgatct
     4741 actagtgatt tggaaattga cccgtctcgg acgcaatatg ttagatgtct tacaaacagt
     4801 cgaggaatta ttgcgatatg acgtaggatt acattcgatc tcggaacaat ttgacgtgac
     4861 cacttcgaca ggtaaattga tgttacaact ccttgggagt ttcggtgaat ttgaacgcaa
     4921 ccaaattgcg gagaatgttc aaatgtcaat gcaaagctta gtgaaagata aaaagcgtta
     4981 tgcaggaggc cgacgtttag gctatgtcag tggtattgac acgaatggtc aaaaacaatt
     5041 ggtcattgaa ccagaagagg cgaaaattgt tcgtctgatc tatgcaaaat atttaggcgg
     5101 agcagggtat cgagcgattg ccaatttttt gaatcgtcaa ggatatcaaa cagtcaaaaa
     5161 gaatgctttt tctaccacgg cagtcaaaga tatcttacgc aataaaattt atgcagggta
     5221 tttagaatat gcgcgctaca tcaattggga aaccaaacga cggaagggga agaatccgag
     5281 accgatttta gtgaaaggaa cccatgaacc gattatttcg gaagaagatt atcaaaaagt
     5341 acaagaacgc cttgctttag aaggaaaaca acccaaatgg aaccatacag gtgaaaatgt
     5401 attgacaggc ttgttacgtt gtccagaatg tggcgcaccg atggcagcga gtaatgtcac
     5461 aaatacccta aaggatggca caaagaaacg gattcgatat tattcttgtt cagtctttcg
     5521 gaataaagga gcaagtgtgt gtcatgccaa ttcgattcgt gccgaacaag cggaaacatt
     5581 tgtcgccgac cgattaaagg agatcgttca gctaccagaa gttcttcccc gattggtggc
     5641 agcgttaaat gaagaaatag tgcgacaaag ccaaccattg gaacaagaac tcgtggtgtt
     5701 actggaacga aaggaagaat tgaagactaa aattgaaaaa tgggaagctg ctttggaaga
     5761 tagtcctgaa ttgtttccga tgttaaaaga ccgcttagac gaattgactg aaaaacgacg
     5821 acagttgcac attcgagaaa atgaaatctt agggatcttt caacaaggag agccgattca
     5881 agtcaaagat gtccaacgga tattgactag tttggatcgt tttcttgccc agagtgagaa
     5941 gaagcaaatc aaagccttgt atcgtacctt tatagagaaa ataacctttg atcctaacca
     6001 taaggagatg ttggaaatta cgatgaaatt cacgcatgcc gtggtacaac aattgaatga
     6061 gcaatatcaa atagccgtat ccaaaacaaa ggatacggct atttttgtgt taaaaacacc
     6121 ctttacgtta acgatctagt ctaggttata tcaaacaacg ttagaggggc gctaacgctt
     6181 ctctagcgat ttgaaatcgt tagagaagta gttagggagg tccttatcat aaatagggat
     6241 atttattaat gaatatttga aatataattg tatataagaa ttaaaaagag tggggataga
     6301 tatgaaaaag atgggtaagt gtattttatg tgaaaaatac actgaactta ctaaagaaca
     6361 tgtaccacct aaaaaatcag ggaatactgg aggtaaaaag accagaactg gtaatttaga
     6421 tgatttcctt aagagcgatt ttacaaaagg tgattttccg aaggggatta aaaggaaacc
     6481 tcaaggaaat gtttattata
//
LOCUS       DAZBTF010000114         6347 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000114 DAZBTF010000000
VERSION     DAZBTF010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.119"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            290..1135
                     /locus_tag="ACHGNS_002718"
     CDS             290..1135
                     /locus_tag="ACHGNS_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN6938587.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1416..2636
                     /locus_tag="ACHGNS_002719"
     CDS             1416..2636
                     /locus_tag="ACHGNS_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFN6938588.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2633..5353
                     /locus_tag="ACHGNS_002720"
     CDS             2633..5353
                     /locus_tag="ACHGNS_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN6938589.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5337..6281
                     /locus_tag="ACHGNS_002721"
     CDS             5337..6281
                     /locus_tag="ACHGNS_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFN6938590.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2363 a          983 c         1224 g         1777 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      661 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat
      721 ttgttcaatc ccttataata atattgagga aattatagaa aaggatgcta tcagcattta
      781 caaatttttt atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa
      841 aaagatgcta aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca
      901 aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc
      961 tttgagaatc gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat
     1021 tagttttgca atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt
     1081 ttcaaatgat aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa
     1141 ttaaggtagt atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac
     1201 aaatagtact tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac
     1261 aaaaatcatg tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc
     1321 gtaatttctt tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa
     1381 tggtatgata aaagaaaaaa aggaggaaat ttttaatggt gaaatttatc catgcggcag
     1441 atttacatat ggatcggtca tttgaaggac tgacaactct tgataaaact gtacaggaaa
     1501 aactattgaa gacgaatctg accgttttat caaatatcat tgaacaggca ataataaata
     1561 acgttgattt tgttttactt gcaggagata attttcatca aaatcgtcct tcattaaaaa
     1621 tacagaaaca tttttcagaa cagatgaaac ggttagaaaa gaaccatata cctgtattta
     1681 taatattcgg aaatcatgat ttctatcaaa aagagcggta ttggttctcc tttcctaaaa
     1741 atgtccattt atttacatcc gaaacggtag agacaaaaaa aataacgata aaaagtggag
     1801 aaactgtctc tctcagtgga tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga
     1861 tggaatttcc ttctagagaa ctcacagatt accatattgg gttgtaccac ggggaacctg
     1921 gcgtctctca aaaaggaaac tatgctcctt tccaaccctc aaagatgcaa gaaaagggat
     1981 acgattattg ggctttaggg catatccatg tgccaacggt cttaaatgaa aagcaaacaa
     2041 ttgtttatcc aggtgcgcct caaggtcata ctaaaaagga acaagcttct acatctattc
     2101 tactagttga attaagtaaa ggaagttgcc aaatacatcc gattaaagta gcagaggtct
     2161 attggaagac aaaggaaata tcattgagga aagcaagaac gacaaaagag gtcattgttc
     2221 acattcgtca gcagctttca aaagtagagg atggatttag tttaattgag ataaaattaa
     2281 aagattatga ccatctcaat acagatgttt tggaacggat acaatcagga gagctattag
     2341 actatttgtt agaggaattc tcagatagga taaatgattt ctttatttgg cgtatttctc
     2401 tcatagagaa tactttttat acgaagaccc cacttgctgc ttctgctaaa ttgatggaac
     2461 agttatttca atactatcag acacctcaag atttccaaca aattttaaat gaagtatata
     2521 gtcatcaaga agcggctaga atattgagcg aactgccgga atatcaagaa gaaacacttg
     2581 aaaaagccaa gcagctgctt aatcaagatt ttttatttga ggaggatcag gaatgaagat
     2641 tttaaggatc gagattgctg cttttggaaa atggcgtcaa aaaagttttg acttttattc
     2701 aggcaatcag ctgatctatg ggggaaacga agcaggaaaa tcaacgattt atcaatttat
     2761 tcaagcaata ttatttggtt tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa
     2821 agacggctca gcctatggcg gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc
     2881 tgtcgagcgc tacaagcagc aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt
     2941 gggaagtgat gagttactag aaaaactgat cgcacctttg acgaaagagt tattccagca
     3001 ggtatttact ttccagcaag agcagttgat ggaactagat catttgagag aaaaagagct
     3061 acatgatgcg ctgatttctt taggcatcac gggcagttct caagtattcc aaaagcggca
     3121 agaatattat cagaaagcac aacaattatt caagaaaaaa ggccaaaaat tactaatcaa
     3181 tcaaaaatta aatgaatgga aagagctgca agaaaaaatt catcaaaaac aagaacagga
     3241 agcttatttt ggcaagttga tccaacaaga acaggaatac gagtcgcaac agcagcagtt
     3301 attggcacaa ttaaaagagg cgaacaaaca attttcatta gccagacagc aagaacttaa
     3361 tttttcttta tatgaagaat ggcaatctct caaaaaaaac agcactacag gtcttcctga
     3421 tgagcaactt cttttagatc tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg
     3481 tttggaagaa ttagaagcaa cactggaaaa gcatagcggg atggatcagc agtcagccag
     3541 atattatttt tatttagagc aggagcagga actaaaagat ctccagcaac ttcaatttac
     3601 agcggaacaa ttaaacgaag aatggcaaag attacaaatt gagactagag aaacaaattc
     3661 agaattgaag attttggaac aaaggtggca ttggcagaaa aatccgttac cggaagaatt
     3721 tttatatgaa gaagaatgga agcatcttct ggctgaagaa agaaggattg ctgatctaga
     3781 aagccagatt tctgtgcgtc tccaaatagc taaagaacag caagagatga ttgaagcaga
     3841 agtgaatgac ttggaagaga cctatccagc attattacaa aatcagtcag agatagcacc
     3901 agtctcttca tctaaaccgt atatgctgat ggctggcgga gcaataatca ttgcaggtat
     3961 atttgtacca atgcccttga aagtgatcct tttactaatt ggtgcaggca tgattggcta
     4021 tttcttctac aaaaggcaac caaaaattaa aactaaaaat gatttctctg agataaaagg
     4081 aatgtggcaa gaaaaactag gacaacttga tctagcctta gctcaattag ctgaagcaga
     4141 agaaaaacaa aagagaactg gagaagaaaa acagattctt cagcaagaag tatatcgttt
     4201 tgcggatcat cggcacttag gaaagatgga tacgctcaaa ctcatgaaag aatatggaca
     4261 ggaagtcata gattatcaga aattatatgg cgtatttgag cggaaacata aaagagaaca
     4321 ggaaatacaa gaaaaactac gacaaatcga ccaaaaattt gagtttgtta aagaatggtt
     4381 accgatacaa gataagacaa tttcagaaaa aatggtattg ctttcccgtt ttatacaaga
     4441 gatggaacag atgaaatttg ccagaagcta tcagcaaaat actttattga agcaagaaat
     4501 caatcaattg aaaaagaaac aaaaagtatg gatacaagaa tacagcgaac aattgactca
     4561 agcaggctta tcttatcctt cggaagtacc tgtcttttta caaaaagcca aacaagccga
     4621 acagcagatg aaaagataca atgaattatc ggaaatgctt actccactat ttcctgaaga
     4681 gctgacaaaa gagaaattat ctgtaagact gcgccaactg aaagaaaagc aatcccaact
     4741 ccagcaagaa gaaaaagaag caagtgaaaa tcggcaacga ctccaacttc aagtagaaga
     4801 gttgcaaaga gacggcacat tggacgaact ttatcaacaa gctagccggc aaaaagcaaa
     4861 attacaggaa ttactggagg aatggggagc acttgaaatc gccgggacat tcttaggtga
     4921 tctttcaaca gagatgagtg aaagacaatt accccagttg ttaagagcag caggtgatta
     4981 tttaggtatt ttaacaaaag gaaattatca gaagatatta ctgaaaaatg atacactgac
     5041 tttaacagac ggatatacag aatttcccat ctatgagtta tctacaggta cgaaagatca
     5101 gctgattatg gcgatgagat ttgcttacct ctcattagaa gctaaccgtt cgatatgccc
     5161 gatcatcatt gatgatggct ggcttcatta tgatcatcag cgaaagtatc aattagctga
     5221 acttttagct aaatttggag aaaagtatca aataatttgt ttttcttctg ataaagaaat
     5281 ggtaagctat taccatgaga tgaaccaaat cgtaaaacaa ttagaaggag caaataatga
     5341 aaaaaattcg tgatttagta atcgatgagc agtttgaaca atttgtcctg atcaaaagtg
     5401 cagatgtaag aatcgctaaa aacggaaaaa aatttattgc ttttactttc caagatactt
     5461 caggtactat cgatggtaaa tactgggatg cttcggagga ggaaatcaat aaatttacag
     5521 ccggcacagt cgtttttcta aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa
     5581 agattcttca tatgcgctta acaagggtag atgagccagg agagcctagt ttatacatgg
     5641 aaagagcccc tttgaagaaa gaagaaatgg aagaagaaat caatcaattg ttgtttgaga
     5701 tcaccaatgc gcattggaat cgtattgttc gctatctact taataaatat caaaaacaat
     5761 tttttgagtt tccggcagca aaaagaaacc atcatgcatt tgccagtgga ttagcttatc
     5821 atactttgac aatgcttcac ttagggaaat caatcgccga tgaatatcct gagctgaatc
     5881 gagcattgtt gtatgctggt atcattttgc atgatttagg aaaggtgatt gaactttccg
     5941 gtcctatgtc gacagaatat acgcttgcag gaaatttgat cggtcatctg gtattgattg
     6001 acgaagaaat aactaaagcc tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc
     6061 tcgtattacg tcatatggtt ttagcacacc atggcttatt agaatatggc tcacctgtac
     6121 gtccaagaat aatggaagca gaaatattac atcaaatcga caatatcgat gcttcgatgc
     6181 agatgatgtt gacttcgatt cgtcaaacag aaccaggaaa atacaccgat cggattttcg
     6241 gtatggacaa ccgaagtttc tatgtaccaa aagatttata gcaaaaattt atttttttca
     6301 ttcaaaacaa aaaaagcgag acataagtct tcgctttttt tgttttc
//
LOCUS       DAZBTF010000115         6318 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000115 DAZBTF010000000
VERSION     DAZBTF010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.120"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..264)
                     /locus_tag="ACHGNS_002722"
     CDS             complement(<1..264)
                     /locus_tag="ACHGNS_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938591.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQS"
     gene            complement(472..1398)
                     /gene="glsA"
                     /locus_tag="ACHGNS_002723"
     CDS             complement(472..1398)
                     /gene="glsA"
                     /locus_tag="ACHGNS_002723"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFN6938592.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1421..2848)
                     /locus_tag="ACHGNS_002724"
     CDS             complement(1421..2848)
                     /locus_tag="ACHGNS_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN6938593.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(2879..4093)
                     /locus_tag="ACHGNS_002725"
     CDS             complement(2879..4093)
                     /locus_tag="ACHGNS_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFN6938594.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(4179..4406)
                     /locus_tag="ACHGNS_002726"
     CDS             complement(4179..4406)
                     /locus_tag="ACHGNS_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938595.1"
                     /translation="MKNKFENRSDTRCGSFLYILYEVFFIPYEKISLKIKKDENKRFS
                     IKRRPFIFCLYLCEKRKRAQHIKLLLIVCLL"
     gene            complement(4434..4676)
                     /locus_tag="ACHGNS_002727"
     CDS             complement(4434..4676)
                     /locus_tag="ACHGNS_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFN6938596.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(4708..5265)
                     /locus_tag="ACHGNS_002728"
     CDS             complement(4708..5265)
                     /locus_tag="ACHGNS_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFN6938597.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(5278..5466)
                     /locus_tag="ACHGNS_002729"
     CDS             complement(5278..5466)
                     /locus_tag="ACHGNS_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFN6938598.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(5479..6045)
                     /gene="amaP"
                     /locus_tag="ACHGNS_002730"
     CDS             complement(5479..6045)
                     /gene="amaP"
                     /locus_tag="ACHGNS_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFN6938599.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         1901 a         1324 c         1078 g         2015 t
ORIGIN      
        1 agattgaccg tacagaaact gtgtgagctc agaaagagca ataccatgtt tgaatacgag
       61 tttatactta tgcttctctc ttacttgttc ttttatacaa tattctagac actcggctag
      121 actttctaga tcgtgaagga tcgttggtgt agtcaccctt agagaaatag ctgtttgttc
      181 gatgtctatt tcttggaatt caaataattg ttcacacaga tacacttttc taaatatatt
      241 tcgctcgatg taattttcca gcattcctga tacacgcctc cagtttttct tatgatttta
      301 tttaatcata tctgtaaatc gtatttttgt ttccttttta tttagaaaag ccctttcaaa
      361 aaccaataaa aaaagaaact gggacaaaag tcaaacaagc tcacaatttc agcctcattg
      421 taggtagctt tgagccgttt ggacttttac cccagcctct ttcaagaatg gttattcaaa
      481 gatgtcagcg tctagttctt tgactacatc tttcaacaat tggataccag ctgcgctgtt
      541 accaaatgga tctagtgccg ggctgaagac acctatgccg taacgatttg gcacagctgc
      601 catcaacccg ccgcctacac cgctttttgc aggtacacca acatggacgg aaaactctcc
      661 tgattcatca tacaaaccag cagttgtcat aattgatttg acgattgtag cactttcttc
      721 agtaatgatt tgtttgccat tccaaggagc aattccttta tttgccagca cagcagctaa
      781 ttttgctatg cctgtagcgg tgacattgag ggagcattgt ctgaaataag catcgagtat
      841 ctccggtacg tctccttcga tcatttgatt acctttcata tagtaagcaa gagagcgatt
      901 gatatcacct gttcttgatt cagaatgata gatttcttca ttcaatgtga tattaggatc
      961 atcacagatc tctttcataa atgacaagat tcgctcaaat ttttcatctg catctttacc
     1021 ggcaatcaac gaggttgttg caatcgctcc tgcattaaca aaaggattca ttggatgttt
     1081 gcgatgattg atttccatat tcatgataga attgaaggca aaaccggttg gttccgtgtt
     1141 gattgtttga aagacttttt ctattccgtt gtctttgatt gctaataaca atacgggaac
     1201 ttttgacatg ctttcgatgg caaagcgcac atcggcgtct cctccctcaa tcagttgttt
     1261 tgtatccaaa tcataaatcg ccatacctaa ttgtttggga tccacttcag ctaatgctgg
     1321 aatgtaatcc gccacttgtc ctgtttgata atagtggaaa ttttttccaa tcaattgttc
     1381 taattctttt acttccattt gaataaccag ctttcatttt ttatttttct gcatgctgga
     1441 atttatgacg gcatgcgtag atcgtaaatg gaataactaa gacgataaga taagaaacaa
     1501 tcagaatcgt ttcataaatc ccatgctgtc cagctgcgat actagcaggt ggaacaaatg
     1561 agatacaaaa agcaaaaagt gaagtcaaaa gacctaaaca agcaacgatt acttttacaa
     1621 cttttccgcc tggaacctga taggctcttt caagactgtc atctttaaaa accagcttca
     1681 aatagcccgc gaaaaacata aagtacaaca tcaggtagat tacaacagtc aatgacatcg
     1741 ctgcaagaaa tgaaagatta ttccccccgc cgcttaatgt aaagacggct gcccagattg
     1801 ttgcaacgat cccatttgcg atcatcaaat gagcaggaac accgtgtgtg ttttcttttg
     1861 caaaatattc aggaagtaat ccttcttctg ctgctacgtg aagtgctttt gaaggagagg
     1921 ttacccaaga agcaatttct ccgatgacac ctaaacaaac aagaatcgca agaattttcg
     1981 caatccatgc aagatttgga tcaatgtgtc ttaataggaa ttctaacgat tgaatcaccc
     2041 ctgtattaag agacaaacca tgttgaggta ctgtagtagc gacggtcaat ccgccaaaag
     2101 tatctaaaat gattgccaga ataaccaata caaacattgc taatggataa tcctttttag
     2161 ggttttccat ttcatttgca tgactggcag atgtttctac gcccatatat gctaaaataa
     2221 aggaaacaaa aacaaccagc gtaggcaatt tcgaaaaatc aggaatcaat gttttcatag
     2281 acagtatcgt ttgtagtgga gcgcctgaag cgacataatg gatccctaag ccaaacaaga
     2341 ctaatgctgg caacaagata ccagctacaa aaccgatccg agcaatcaat gaagtgtatt
     2401 tggttccttt taactgtgag agtaaaatag cccagaaaat aatcaatagg attgccaatt
     2461 tcagatattt attgttttgt atagcaccaa tacccgttgc ataggataat gcaccagtta
     2521 agaaatacag catggtcaag tagccaactg ttatctcaaa ccattggaaa aagattgcgg
     2581 caaaaccgaa tcgttttccc aatgtgcgac tgacccaagt atagacaccg cctttttccc
     2641 atcctttcac cgttgccatc tctgctgcgc acaatgccac agggataaac catagcaaac
     2701 cgcctaataa caagaaaaat actaagctga agcctgaggt cgcgaatgtg ggatattcat
     2761 ataatgaaac gaccattgag atggtcatag ccaaaaagcc aaagaacgtt aatttttttg
     2821 caggaatttt cgttttgctt tgttgcatga atcttcctct tttctatttg aatttgtttc
     2881 aattgagttt gttgccaaag aagaaaaagt ccaatccttc ttcttgttct tcttttgtag
     2941 tagtgatcgt tgtaaagtgc atcgccgatt ttaaaatcac tgttgtcagc acataggcat
     3001 acacggcagt aatgatgaca ccaatcactt gaatccctaa gaagtaccag ctgcttggat
     3061 tcgttagtaa atggctgtta gatgcaaaga tccctatcag aatcgtacct gtgaatccgc
     3121 ccatgccatg aacaccccac acatcaagtg catcatccca ttgtttcttt ttacgaaaat
     3181 caacagcagc atgacagacg atacccgcaa tgatcccaat cagcatggca ctttttgctt
     3241 ctacataacc ggcacaaggc gtaatggctg ccaatccagc aacggatcct gttagtacgt
     3301 cgacaaaatc aaagccattt ccttttactt ttgcaatgat cagccaagta atcatcgcaa
     3361 atgcaagagc gatgaatgtt gaaccaaatg cattgactgc ttgttcatct gctcgaagtg
     3421 caccaccgga attaaaaccg aaccagccaa accaaagcag tcctgtaccg atagcagctg
     3481 ccataagact gctatgctga cttctttctc ctaaaggaat ctttctttga ccaagtgtga
     3541 ggatacatgc taaagagccg aatccagctg tcgtgtggat gactgctcca ccagcgaaat
     3601 ctctaaatcc catctgatcc aaaaatccgc caccccatat ccagtggcaa acagggaaat
     3661 aaatcagcag attccaaaaa ataacaagta taatatagcc ttttaaattc aatctccctg
     3721 caaaagctcc tgtcatcaaa ggaacggtaa tcacgataaa catcagttgg taaagaaaga
     3781 acattaaaaa tggaatcgtt gcaccatgaa acatgttggg gaagcttgta acatgtctca
     3841 tcaaaaagaa atctgccggg tttccaataa caccgccgat atcccgtcca aaggccaatc
     3901 cgaatccacc aaatacccac ataatcgttg ttatcccaac ggagagaaaa gattgagaca
     3961 tgatcgttag tatattattc ttccgtgcta atccgccgta aaaaaaagct agaccaggag
     4021 tcatcaggca aaccattgct gtacataaaa tcatgaatgc tgtatctcct gtattgatag
     4081 gtaatgtatt catttttttc gcctcctttc tacgattacg agtataaaag aaggtgtagt
     4141 tgtttttttc ttctgttttt ttgaattaat acttgcattt ataaaagaca gacaattaat
     4201 aaaagcttga tgtgttgtgc tctttttcgt ttttcacaca aataaagaca aaaaataaac
     4261 ggtcttcttt ttatagaaaa ccgtttattt tcgtcttttt ttatttttaa agatattttt
     4321 tcataaggaa taaagaatac ttcatataaa atatataaaa aagaaccgca gcgagtgtcg
     4381 ctacggttct caaatttgtt cttcatttca acataagttt ttcttcaatt tgcttataat
     4441 ttacgtgcaa agaatgaaac gactaatact aaaatgattg caccgatcaa tgatggaatg
     4501 atagccattc ctgcaattgt tggtccccaa gttcctagta aagcttgtcc aattgctgaa
     4561 cctactaaac cagcaatgat gtttgcgatg atccccattg atgatccttt attcgtgatt
     4621 gctcctgcaa ttgctccaat gataccgccg acgattaatg cccaaataaa tcccatttta
     4681 aattcctcct aactgacttc ttacttctta ttgtacacgt ggttcgttat tttctttgac
     4741 tttttgtgca cctttgccta cagcagattt tgctttatct gtttggcgtg aagcaaattg
     4801 tcctgtggat tttgctgctt ctgttacttt gtcttgtact gtttcttgat ctttttggta
     4861 ttcttcttgt gttttgatgt ctgcaacatt cacattcact tcgataacat ctaaatgtgt
     4921 catattgttt acttcagtac tgatcaattc tttgatttgg ttgtaaatat cttcgatgtc
     4981 tttaccgtat tctacgatca catctagatc cacagctact tgtttcttac ctacttctgt
     5041 attgatacct gctgttacgt tatctgtgtt gactaatttt tcagccatat ttgagaaaaa
     5101 tccgccatct aatgtcaata gaccatctac atctttcaat gcataaccga tgattttttg
     5161 gatgactttg tcttcataag tcaattcacc attcatcatg cggttttctc ctttttcatt
     5221 tgccaaaact gatccatttg ttgtattcat gtttttgttt tccatctttg tttcctccta
     5281 tgatttaaaa aattgatcaa ctattcctga ctgtttcaaa taaaaaccga ttcctgcgcc
     5341 aattgctgtc atgattatga tcaacaatgt cttgaaaaat ccaatcgtta accataaaac
     5401 agctagcagt agtccaagac ccccaaagat aatagggagc ttgtatgctg aaaatagttc
     5461 tttcattatt tggccctcct attccactcg agagtgcttg tagtctcttg tatcttcttg
     5521 ttcttcataa ccgctgtatg tgactgccac tttgaccgtt tcttttgttc cgagtacttt
     5581 agttacttct tcttcgatat cttgcatcaa tacacctgtt ttccctacta atgaagaagt
     5641 tcgtttcaag tctccgttga catggatatg gattcgattt ttcgttgaac gtatccgtac
     5701 tttaggtgaa tgaatgaact cttcttcgtg taaatgggaa cgtaccaacc cttcgatcgc
     5761 ttttttgtcg attgttagtt tgccatcttc ccgtttcata acgaaagttc ctcttgactt
     5821 tggatagaaa agaacgacta gcaagatgat aatagcaaga acggcaaata ctacagctgc
     5881 ccagaaaata tattgttgca tgtagtaatt cgttaaagta aagaagcgaa atcgttccaa
     5941 ttgttcaggc ataatatacg gcgcattgat caacgcaatc gctagaaata ctgaaagtag
     6001 cagtaaactc atgataacac cgattatttt taacccctta ttcattttcc acctcctata
     6061 aacttatttt taccctgtaa ataactctac cgacaatcac agtatagata gcgaacagtt
     6121 agcatagcat cagtaaataa agcgctacca aacattgctg gatcataacg gcgttttatt
     6181 gccagtctga tactgaaata acaattgctt acttattctc tatttacttt tgattgctgg
     6241 ctatacttaa aggatattat tcaagattaa aaattgcaaa gattatgctc agttattatt
     6301 acaagattct atttattt
//
LOCUS       DAZBTF010000116         6281 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000116 DAZBTF010000000
VERSION     DAZBTF010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.121"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(42..230)
                     /gene="rpmB"
                     /locus_tag="ACHGNS_002731"
     CDS             complement(42..230)
                     /gene="rpmB"
                     /locus_tag="ACHGNS_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFN6938600.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            500..862
                     /locus_tag="ACHGNS_002732"
     CDS             500..862
                     /locus_tag="ACHGNS_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFN6938601.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            914..2596
                     /locus_tag="ACHGNS_002733"
     CDS             914..2596
                     /locus_tag="ACHGNS_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFN6938602.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2713..4749
                     /gene="recG"
                     /locus_tag="ACHGNS_002734"
     CDS             2713..4749
                     /gene="recG"
                     /locus_tag="ACHGNS_002734"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFN6938603.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEGSEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4798..5799
                     /gene="plsX"
                     /locus_tag="ACHGNS_002735"
     CDS             4798..5799
                     /gene="plsX"
                     /locus_tag="ACHGNS_002735"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFN6938604.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5921..6163
                     /gene="acpP"
                     /locus_tag="ACHGNS_002736"
     CDS             5921..6163
                     /gene="acpP"
                     /locus_tag="ACHGNS_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFN6938605.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT         2088 a         1093 c         1432 g         1668 t
ORIGIN      
        1 aaaaaaccgt tcactatctg aacggtccaa gtatcattca attaaacgcg ttcaactttt
       61 cctgatttca aagcacgagt tgacacccaa acttttttag gttttccgtc gaccataacg
      121 cgaacttttt gtaagttagg tttaacagta cgttttgttg agttcatagc gtgtgaacgg
      181 ttgtttccac tttttgtctt gcgacctgta aagtaacaaa cttttgccat ggtaatttcc
      241 tcctttgcct tgatttggag caatagaata attcagctca taccagacta acatatcata
      301 tagaatggaa atttgcaagt acattttcat agatacaatt gacaattgag aagaataatt
      361 gtaaattaaa caaggttata cctgaaattt tgcaaggttt ttgagtactt tgggtaactg
      421 ccttttattt caagttgcgc tatgataaaa tatgatagaa gaaaaatggg agttttttac
      481 ccgttaagga ggctcattca tggctgtaaa aattcaaacg tcagctggaa ccattgaaat
      541 taccaatgat gtaattgcaa cagttgttgg tggcgccgcg acagatgtgt tcggtgttgt
      601 aggaatggcc agcaaaaatc aaattaaaga taatattact gagattttgc gtaaagaaaa
      661 ttactcaaga ggagtagttg tccgccaaga agataatggt attgctgtag atgtttatac
      721 gattatcagc tatggtacga aaatctctga agtttcccgc aacgtccaag aaaaagtgaa
      781 atacaatcta gaggcattat taggcgtaac tgccaattca gttaatgtct ttgtacaagg
      841 tgttcgtgta ctgccagact aggcaggaga ctggctggta actcagcagt cgatctgaag
      901 gaggattatc taggtgagtg taacagaaat aagtgcaagc cagttccagg aaatggtcca
      961 ggcaggtgcg aatcgtctgc aagtaaatgc agaatacgtc aattcattaa atgtattccc
     1021 tgtgccagat ggcgatacgg gaacaaatat gaatctatct atgactagtg gagctaaggc
     1081 agtagtagat tcaacctcag aaaaagtagg agaattagca gcaatgcttt caaaaggatt
     1141 gctgatgggt gcgcgcggaa attccggcgt tattttgtct caattgtttc gtggattttc
     1201 taagcaaatt ccagatgttg tctcattaaa tgcttccgat cttgctgcag ccttcactca
     1261 tggagttgaa actgcgtata aagcagtaat gaaaccagta gaaggaacga ttttgacagt
     1321 agctagagaa gcagcgaaag ctggagaaaa gaaagcaaaa agtactgatg atgtcgttga
     1381 agtaatgaca gctgttgtaa aaggggcaaa gcgtgcttta gctaaaactc cagacttgct
     1441 tccagtccta aaagaagtcg gtgtcgtaga cagtggaggg caaggactgc tgtttgttta
     1501 tgagggcttt ttaagtgcat tgaatggaga attccaagcg gatgaaacat atgaaccttc
     1561 tccagcagaa atggatgata tggtcaatgc agaacatcat cgcagtgtcc aaggtcaaat
     1621 ggcgacagaa gatatccatt ttggttactg tacagaaatc atggtaaaaa tcggagaagg
     1681 acctacagtc gatagtacat tcgattacga agaattccgt aactatttag atggtatagg
     1741 agattcttta cttgtggtca atgatgacga gatcatcaaa gtccacgtgc atacagaaaa
     1801 cccaggagaa gtcttgaatt atggacaaaa attcggttca ttagtcaaaa tcaaagtcga
     1861 caacatgcgc ttgcagcatg agacgatttt ggaacacgat gaagaagtga acgagtttgc
     1921 gaaaaaagaa acaccacgta tcccgtatgg tatcgtagcg atcgctgctg gtaaaggcgt
     1981 ccaagagctg tttgaaagtt taggcgctaa ttatgttatc agtggtgggc aaacaatgaa
     2041 tccaagtacg gaagacattg ttaaagcgat tcaagaagta aacgcagata aagtgattgt
     2101 cttgccaaac aataagaaca tcttcatggc agctgatcaa gcagcagaag tagcagatat
     2161 cccagtagca gttgttcctt caagaacgat ttcccaagga ttgacagcta tgctttcatt
     2221 caatgatcaa gcttctttag aagaaaacaa agaaacgatg acagaaatgt tgtctagcgt
     2281 tgtgagcggt caagtcactc acgcgatcag agatacctca attgacggtg tagagatcaa
     2341 agaaggagac tttttgggca tgatcgatgg aaaaatcctg atctctaacc cagatatcac
     2401 agcaacagcg ttaagtacac tagaacaaat gatcaatgag gacacagaga tcgtaacgat
     2461 cttggcaggt gatgaaggaa cagttgaaca agcaaatgaa tttgctgaga aactaacaga
     2521 aaaacatcct gatttggaaa tcgagatcca ccaaggagat cagcctgtct atccttatct
     2581 attttctgct gaataatttc tgtgtcagaa tatctttcta acacagtatt cattcataaa
     2641 aggccttcgg ttagtgatca ggtatgatcg ctgaccaagt cctttttttg ttaagggagg
     2701 gaggaaaaat ttatgcgttc tttagatgat ccaatcacta tgctggcagg agtcggagaa
     2761 aaaagagcag ctagtcttgc cagcttgggc atccaaacga ttgaagactt actgacttat
     2821 tatcctttcc gctacgaaga tattcaggaa agaaatctaa acgagatcca agatcaagaa
     2881 aaggttactt tgaaaggaat cgtagtctcg cctcctgtga tgaatcgatt tggttataaa
     2941 aaaagccgat tgcaatttcg tatgatgcaa gaacatgatg tgttcaacgt ttcttttttt
     3001 aatcaacctt atctaaaaga aaaagtcatt ttatcggagg aaatcgccgt atacggtaaa
     3061 tgggatgcaa agcgtaaagc attgaacggg atgaagattt taggttctca aacaatggat
     3121 gacttttcgc cgatatatca cgtcaacaaa tcaattcgtc aaacgacatt agttgatcta
     3181 atcaaaaaag ggtttcaaga atacggcaca ttgattgaag aaaatctgcc aaacaacctt
     3241 gtggagaaat atcgactgct tgaccgagct tctgctgtaa gaagtatgca ttttccaaaa
     3301 gaccacgaag aaaaccatca agccaaacga cgtgtcgttt ttgaagagtt ctttttattt
     3361 caaatgcgtc ttcaaggatt gaaaaaagca gaaaaagcag aaacgaatgg tttggagatc
     3421 ctttatgatg tccaaaaatt gaaagaattt actcaaaagc taccttttga actgacaaat
     3481 gcacaaaaac gtgtaacgaa tgaaatctgt cgggatcttc gcagtccgca gcatatgcag
     3541 cgacttttgc agggagatgt aggtagcgga aaaacagtgg ttgccgcgat tgcgttatac
     3601 gctaccgtta cagcaggttt ccaaggtgcg ttgatggtgc ctacagaaat cttggcacaa
     3661 caacatatgg aaagcttgca acagctgttt gatcccaatg aagtaaccat cgcattgctt
     3721 acggggtcaa cgaaagcaaa agaacgtcgt gagctccttg aaaaactaga gcaaggggaa
     3781 attaacatcg tgatcggcac acatgcattg atccaagatg gagtcgaatt tcagaattta
     3841 ggtctagtta ttacggatga gcagcaccgt tttggcgtga accagcggaa agtacttcgt
     3901 gaaaaaggct ggcgaccaga tgtcttattt atgacagcga ctccgattcc tagaacatta
     3961 gctattacag catatggtga gatggatgta tctatcattg atgaacttcc agctggacgt
     4021 atcccgatcg aaacacgctg ggtaagaaca cctcaattgg acagtgtact ggattggacg
     4081 aaaaaagaac tggcaagagg acatcaaatg tatgtcatct gtccgctgat tgaaggatca
     4141 gaagcattag atgtaaaaaa tgcagtggaa atctatgaaa aacttcgtga actatttgca
     4201 ccaaaatatg aagttggctt gttacatggt aaaatgaaga atcaggaaaa agaagtaatc
     4261 atggaagcct tcaaagacaa ccagatgcag atattagttt cgactactgt aattgaagta
     4321 ggggtaaacg tgccgaacgc gaccgtcatg ctgatcatgg atgcggatcg atttggattg
     4381 gcacagctgc atcaacttcg tggacgtgtt ggacgcggaa gtgatgcttc ctactgtatc
     4441 ttagttgcta atcctaaaaa tgaactaggt gtggaacgga tgaaaatcat gacagagacc
     4501 accaatggtt ttgtactaag cgaaaaagat ctggaattac gtggtcctgg tgaagtattt
     4561 ggcttccgtc aatctggtct gcctcaattt gcgattgctg acttggtgac ggatggaaat
     4621 gttttggagg tagccagaga agaggctgca gccctttggc agataaagga ttggcagatg
     4681 cttcctgagt atcagccttt agcgcaaagc ctccaaatag aaaatcagga taatcgttat
     4741 ttcgattagc agactgtgct atactttata ctgatgacgg aaaaaggagt ggaaacaatg
     4801 aaaattgccg tagatgcaat gggcggagat cacgcacctc aagcaattgt agaaggcgtg
     4861 gctttagcgc agcaagattt tccagaagta gaattcttac tttacggaaa agaagcagaa
     4921 atcaagaaat acttaacaaa tgaaaaaaac atcacgatta tccatacaga cgaaaaaatc
     4981 aatagtgatg atgagccagt aaaagcaatt cgacgaaaaa agactgcatc aatggtcctt
     5041 gctgcacaag cagtgaaaaa cggtgaagca gatgctgtat tttcagcagg gaatacaggc
     5101 gcgctactag cagcagggtt gttcatcgta ggacgtatca aaaatatcga acgccctggc
     5161 ttgatgtcta ccttgccagt agtagggaaa gaaggtgccg gtttcgatat gctggatctt
     5221 ggcgctaacg cagaaaacaa accagaacat ttgttgcaat acggtatttt aggttctttt
     5281 tatgcacgta aagtccgagg tatcggacgt cctcgtgtcg cactcttaaa caacggaaca
     5341 gaagcaacaa aaggtagtga agtgacgaaa aaagcctatg aactccttca gaatgaaaca
     5401 agcttgaatt ttattggaaa cgtagaagct cgtgagctac tcaatggtgt ggctgatgta
     5461 gtggtcacag atggatttac aggcaacgct gttttgaaat ctattgaagg aacagcaatg
     5521 aatatgatga atctattaaa atctgctatt ctaaatgaag ggatcaaagg aaaaatgggc
     5581 gctatgcttt taaaagacgg cttacgttct ttgaaagcag aaatggatta ttccaaacat
     5641 ggcggtgccg ttttgtttgg cttgaaagca ccagtcatta aaactcatgg agcaacaggc
     5701 cccgatgctg ttcgctatac gatccgccaa atccacacaa tgcttgagac agatgtagtt
     5761 ggacaattag tcgaacaatt tgaaaaaagc gaagaatagt atttggatat ctgaagcaat
     5821 ccgttccatt gaaaaaaagg tggacattcc tgaaaaatgc cagtaaaatt agccatggtg
     5881 taggtaattg acaagtgtat gattcttgga ggtgaaaaag ttgactcgcg aagaagtatt
     5941 taacaaagta gcgaaaatta tttcaaatca ttttgagata gataccgata aagtgacgga
     6001 tgaattgaac atcaaagatg atttaaaagc tgattcaatt agtattatgg aattcgtttt
     6061 agagcttgaa gatgaattcg gtacggaaat ttcagatgaa gacgctgaac aaattgaaac
     6121 agtcggtggc gcagtagatt atatcagcag ccatcttaaa taatttcaaa taaaagcaaa
     6181 gaaggataaa aagctgcata cagcttttta tccttctttt tttaatcttt ttgacacgta
     6241 aaagtcattt ttctgataat tctggcgata aacagaaatt a
//
LOCUS       DAZBTF010000117         6224 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000117 DAZBTF010000000
VERSION     DAZBTF010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.122"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(207..1169)
                     /locus_tag="ACHGNS_002737"
     CDS             complement(207..1169)
                     /locus_tag="ACHGNS_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN6938606.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="ACHGNS_002738"
     CDS             complement(1171..2610)
                     /locus_tag="ACHGNS_002738"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFN6938607.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="ACHGNS_002739"
     CDS             2795..4741
                     /locus_tag="ACHGNS_002739"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFN6938608.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            5005..5877
                     /gene="scrK"
                     /locus_tag="ACHGNS_002740"
     CDS             5005..5877
                     /gene="scrK"
                     /locus_tag="ACHGNS_002740"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFN6938609.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         1986 a         1061 c         1107 g         2070 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaggc acatttaaaa ttgatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta
     4861 ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca
     4921 tgttactaaa taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat
     4981 tctaaaataa agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa
     5041 tttgtgtgtg ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca
     5101 acgacacctg aggaaacaat gcctttagtc attgacttct tcaaacaata ccaagcagat
     5161 ttagcaggga ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact
     5221 tatgggtaca ttacctcgac cccgaaattg gcatggcaaa attttgattt catcggtacc
     5281 atgaaaaaag aatttccgat cccgatttct tggacaacag atgtcaatgc ggctgcctat
     5341 ggtgagtatg tcttcggcag tggcaaagga ttgagcagtg tcgtttacta tacgatcggt
     5401 acgggtgttg gcggcggtgc cttacaagaa gggcgcttta tcgaagggtt tagtcatcca
     5461 gaaatggggc atatgctcgt cgtccctcat ccaaaagatg actttgctgg cagctgtcca
     5521 tttcatggca actgtttaga agggatggcc gctggacccg ccatcgaaaa ccgcttaggg
     5581 aaaaaaggac aagaggttgc agaagatgat ccttattggg aaatcgaagc ctcctatatt
     5641 gctcaatgtg cctacaatac aaccttgatg ttttctccag atgtgatcat ttttggcggt
     5701 ggtgtgatga aacaacgcca tatgctgcaa aacgtccacg acgcatttgc aaaattggtc
     5761 aatgggtatg tcgaaacacc tgagttaagc aaatatatcg tgaccccagc attagaagac
     5821 aacgctggaa cgctcggttg tttggcattg gctcgagaag cggtcttaca ttcataaacg
     5881 aaaaaaggct cttcgacatt tagtgttggt agagaaagat acaactgaaa atttagtcat
     5941 aaccagttag gcagagtgct taactggttt ttgttttact tggaagatca agccttgaat
     6001 gagataaatt cttgctcgaa agttatggaa atttcgatag ccgtaggcca cttgtattgt
     6061 gcaatagttt tttacaaaaa aaagggggtg cggatatttt tttggacctt tttaacaaat
     6121 aggttctgtt gcaaagtttt aaatctacta tcagataagg tagaataata gaaaaagata
     6181 gcaggaggaa tgacgatgag tcattttaaa ggaaagcaat ttca
//
LOCUS       DAZBTF010000118         6176 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000118 DAZBTF010000000
VERSION     DAZBTF010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.123"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            139..225
                     /locus_tag="ACHGNS_002741"
     CDS             139..225
                     /locus_tag="ACHGNS_002741"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFN6938610.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(346..1035)
                     /locus_tag="ACHGNS_002742"
     CDS             complement(346..1035)
                     /locus_tag="ACHGNS_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938611.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1280..2194)
                     /locus_tag="ACHGNS_002743"
     CDS             complement(1280..2194)
                     /locus_tag="ACHGNS_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFN6938612.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2176..2556)
                     /locus_tag="ACHGNS_002744"
     CDS             complement(2176..2556)
                     /locus_tag="ACHGNS_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFN6938613.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3006..3293)
                     /locus_tag="ACHGNS_002745"
     CDS             complement(3006..3293)
                     /locus_tag="ACHGNS_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFN6938614.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3313..3537)
                     /locus_tag="ACHGNS_002746"
     CDS             complement(3313..3537)
                     /locus_tag="ACHGNS_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFN6938615.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(3992..4162)
                     /locus_tag="ACHGNS_002747"
     CDS             complement(3992..4162)
                     /locus_tag="ACHGNS_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938616.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4177..4710)
                     /locus_tag="ACHGNS_002748"
     CDS             complement(4177..4710)
                     /locus_tag="ACHGNS_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFN6938617.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(4731..5675)
                     /locus_tag="ACHGNS_002749"
     CDS             complement(4731..5675)
                     /locus_tag="ACHGNS_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFN6938618.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
BASE COUNT         1633 a         1153 c         1043 g         2347 t
ORIGIN      
        1 cgccttacag gcggtcaggg caagccctaa caaccctaaa ataagcgata cttaaaaaca
       61 ccacacaagt ttaatttttt aagatataat aacttgtcgc ctatccgaca gtcttgcgac
      121 tggaaggagg tgtatctaat gtttgaattg ttcatttgcc cccttcttat agaagtttgt
      181 gtctctttat tcagttattg gttgaataac cgaaacaaga agtaaaatca ataaaggcga
      241 catcaacctg aaaaaaagcc ccagagaatc cgacctctct ggggctttcg tgtatctaat
      301 gtttgatcat ttgcatgttt agtttaacat gattgccgat caaattcaat tttgctgttg
      361 ctgatctaac cattccttga cactttttag ataatttaat gccgtttctt ttttcgttgg
      421 attttgtatg ttcgttgctt gctgaagcag tgctttataa cgttcgtagg taactgattg
      481 ttgccgttct tttcgttcct gttcactagt tgaaaaaggg attgtatcac tgaataaatt
      541 ataacaagct tcttctgtct gcgtatcttt aaaatattgc acgtcttttt tggttcgctc
      601 atagttgtct tgaacctctt tttgctgttt ttctggcaag ttttgataaa gacgagtgag
      661 tttttccatg tttttatccg tcaaacgttt ttcttgattc acaaaaatat cgttcatttc
      721 ctcataacaa gtggtaaact cttccatttt ttgtgttaac acttgctcct ttttcttttt
      781 atcacatgct gcttcatact tttgagtgat atcccctaat aaaatacttc ttgaaatacg
      841 tttatttccc tctaaaacgc cgaataaacc acttaaaacg attgataatg taaatataac
      901 ccctggcaat aaaaacctct taaatcgaat tttagagcct tttaatgaat atcttgtttg
      961 ataagcctta tagctaagcc aaataattcc taaaatactg gcaaaaatga gtagataggg
     1021 taaatagaat gccatttcta tttattctcc ccataccagc caaaaggcta acagtgtcat
     1081 gattaagcaa cataaggggg caagaatatc cattgatcgc tgcataatta cttttttctg
     1141 ttctttattt cgctggttta atagcgcatg gtggaatttt ttgacttgtg aatcaattgt
     1201 agtttgtatt ctttctttgg tataaggtgc aagaaaagca cgttgtttct ttatttcatt
     1261 ttctatgtct tgtaacgttt tagaacgata attcttgttg gtagtctgct cctctttcaa
     1321 cgtgttggtc atggtttctt cgagtcggtc taatttccgg ttgttcagtc gtaagagttc
     1381 gttgttctct tcgtacttct tttgcagttt cgcgaatgct agctcgagct cttcgtagct
     1441 cgttggttcg ttgttgattg taggctcgtt ctcgtctgtc cagctcatgt aaaatcgtct
     1501 ccttttcgta atcctttcct agtttgttgg atcgtacttt tttctttttg tggatcaatt
     1561 cgtaagtaaa atttttccca cgattcaagc aatgaatgcc gaatgtttta agagaatcac
     1621 aaaattggtt atacgttcga gtttttttgt ttttcattgt ttgatcaatc gcaaaccgaa
     1681 tttcgtcttt ccaaacaggt tcattttttt cttttaattt gatttcagac atggttcgtt
     1741 tttcgggttt cttccctcgt tcagtgattg ttaagtcatg ttcaagacaa atctcgtcat
     1801 ttaggtcata acatttgttt aaaaatgctt gaccatgggc ttgaaatttg ttcccagttt
     1861 ccagattgac ggagttaatg acaatgtggt tatgaatatg gtctttgtct gtatgtgtgt
     1921 aaatcgcgat ttggtggtca ggggctattt ttttcgcata ctctgcccct agttgattgg
     1981 ctttttcagc ggtaatttct cctggtcgaa aagactggat caataagtgt gcttgtacac
     2041 gatcgtcttt tccatacagc atgcgaattt gtttcatttc gcttttggca taatcaatgt
     2101 cacagtttat ccctgttttg acggttgctc gtggttcggc gtaattgatc gctcggctac
     2161 aagaagtggt tcggcttact ttaactgttg ccatatcttt tgtagctcct tttgcatgat
     2221 ttttaggttt tcggataagc gttgggcgtc gccaggatca acgtcgtgtt ggtgtgtgtt
     2281 gcaccacttg gcaatttgat tgatattccc gccagctttg gctaaatcgc gcgcgattgc
     2341 ctgagcaatg tctggcgcca ctttcggtgt caccagacgc gcgttttgtg ccttggcttt
     2401 cacgaaagcc ggcacactca tttgcaaagc ttcagctgac tgcttcagct ttgcaaattc
     2461 ggattcgctc actcgaaaac tgatttgttt tggctcggat cgataggtat attttttaga
     2521 atgattgcta gccaaatatt gattttgttc gctcataagt cccacccttt cagtacgtcc
     2581 tacggacgta tcgttcgcaa taccattgta cctaaaaagg tacaataagt aaagctcact
     2641 aaagctcact aaagggcttg gcaagctcta tatttgctag ccaaatacct acgtatttgg
     2701 tgcaaataac cgcttgtcaa aggggcgcaa gctccccctt gaatccccct cccccccact
     2761 ctccacaata caaaaaagac tagcaaatgc tagccacttt ttgtaaagtt tcgcttgttt
     2821 caccttcggt gaaattgttg caaatcaata attaggtaga gctacatttt tttcgttgtt
     2881 caaagattct tatgccattt actattaata aatctataaa tatgataatt tttcggctct
     2941 ataatttatg ggactgatga atcagaattg attcttcagt cccattttca ttttattcca
     3001 aagaattaac tccaaatacc aatagaagcc catcctctat atacttgaga ttccttacta
     3061 acaatttctt gtaattctat taaagattta ggtaatccaa atttatgttt taaaatatat
     3121 cttccaagaa aactacttaa tttcatacta acttcaccaa cactttttcc ctcactgatt
     3181 tctttagaat aaagaaagag cttttctttt aagtcaggat agtcatctat agttgcttca
     3241 ttgtatactt tactaattgc atttaaaagt ttttcttctt tagtaaaatc cattataata
     3301 acctccctac cactaccttc taggtgccca tggaccatga ttaacccaac cattaacaat
     3361 aatttttcca atttctcctt tagcttgatt ccaatctacc caacattttt ctttgttaca
     3421 ataaagacca tttccatagt aagtagctgc atcaactttt attcctgtac cgatgccagc
     3481 tagacaagtt cctaatattc ctagaataac acaatgtttt aatactttct ttttcatgat
     3541 tgtttttcct tttcgttttt ttaaaattgc ttgttatatt aaataatctt agtaatttta
     3601 ttatataaca ttttattata taacaatgat taacgtcata aaaagtagtt ttgaattcat
     3661 aatcgtttaa ataaaattat aagatcagtt atttaaacag ctagtagaca taatgtgtat
     3721 actaccaagt aacgcctgta ccaagggatc caggcgctga ttttgtgaat ttttattggt
     3781 ctaggttaaa agctcttttt aaggcttggt tttctgcggt tgtttctttc taattctttg
     3841 tgaatattga tcggtcattt tttaaatact tgttattttt gtttgtgttt gttatgcttt
     3901 ttacatatct catattcatt tattattctt ttctaaggtt aataatagga taatctcttt
     3961 cagaaagact gctcgtacga gcagtctttt cttatttatc aagtttttct acaaatactt
     4021 cctcattttg actgttgtag taattaggat tgagttttag ttcccctttt ttatttcctt
     4081 tgttggccac tgataataac attgggctaa agcttaatat tcctagtagg atagaaccga
     4141 atagcaagtg tttttttgtc ataagtattt ccttcttcat ttccatagct gccaccattt
     4201 tttccaattc tttgtttgtt cgtccattgt ttctgttgct tcagaaagag tcgttgaact
     4261 attctctgaa gactcttccg tataagtcaa ctgcagttgc tcctgaagtt tttcgatttg
     4321 ttggttggct tgcagggtga gttgttgttg ctggtcgagt agtttttgta tatgagaatt
     4381 attttgctct ttgagggcaa gttgttctcg taaaaattca ttttcttcac gcaactgatc
     4441 tagcaacttg tttgcaacca cgtttttgtt tggtatataa gcatttttat cgttgttttg
     4501 ttcatgttta tgcaacttat ctagcaacct ttgattgtct aagttgcgag caagttgcaa
     4561 tactccagaa cgcaatacaa ccttataacc acgctcattt ttagctatca tggaagaatc
     4621 taatttttgg atatgtttcg atattgcttg cctactcatc ttaaaaagat ctgacaattc
     4681 ctttattgta aaggtttctt gtggttgcat actggttgcc tcctaggttg ctactggaat
     4741 aaattatcta aatcagataa taaacttttt ttctcttttt ccgaaagtgc ggactctgtt
     4801 tgtttttcaa gagctttttt ctctttttct agttgtttga tttgttggag gagttcaagt
     4861 tctcgtttct tctgtttgat tttttcgtta attgttgtag cgggttcttc gtattctttt
     4921 tgtttatcag ctaaagccaa tgctttattt tggtcttctt tggttggtgc atgtttgtta
     4981 aagtcgtctg cttttttgat ctcaggtttg aacgtaaatt gatagccaat aacaggtttt
     5041 ccccgtcctt ttccgtattt ctttttgatt gttagtcctt tgaagattgc agttagttct
     5101 tgacgtatag gttttataac tcgtttatct atatcaggtt gcttataact tttaggaata
     5161 tctaataatt caagaaagtc ttctttggag aaatatgcga aacctttggt tctgtattgc
     5221 tttaacagac gaaacatagt ctttgcatag ccactttgta gctcgttgaa ttgttgtaaa
     5281 gaatagcgta cccactcgtc taaattattt aacaaaggca gtgctttttc gtaaattcgt
     5341 atatctacgt aaggattttc tacgtccccg ttgattttaa attctgtaaa cattacaaac
     5401 atatcgtaag aaagcccaga ctcacttcta cgaccaaagc gtaaagataa gattttttca
     5461 taagttgctt ttaaatcttc tacaaatcgt acatttgcag ttgctttgta gttacttaaa
     5521 tcttttagtt ggtcaaacgt aaaacgaact gtcttatccc ctttttcacg cattctagac
     5581 acgatggaaa aaaacaaatt catttcaacc ggtgtaaatt ttcttaaagg aattgtattt
     5641 aattcatggt ggtattttac aatctcatta gccatataat cacctcaatc caattatgaa
     5701 tgacattttt taaatgtcaa gacattcata tttgacgttc aaacagacaa aatgtccttc
     5761 ataaacagac aaaatgtcct tcataaacag acaaaatgtc cttcataaac agacaaaatg
     5821 tccttcataa cattctgtat ccattggtag agtaaggaca cagcttacgg taaagaggtt
     5881 taaaaagagg tttaaaaaga gatataaaaa gaggggggct aattttcccg cctaaaaacc
     5941 acttcttttt tctttaaaca ggttcgcgca tttttcaaaa ctgccaatcc aaaaccttgc
     6001 ttcctacggt cgccaaaaag gtgtgatcgc tacgctctca cattgtactg cgcagtcgcc
     6061 taaaggctcc tttgctaaaa acatgttggt aaagaggcct ttgagcggtt ctgaatgctt
     6121 atctaagccg ttttaagaca gtcttggata aaaatcctta tctagcttta aagcgc
//
LOCUS       DAZBTF010000119         6005 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000119 DAZBTF010000000
VERSION     DAZBTF010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.124"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            59..415
                     /locus_tag="ACHGNS_002750"
     CDS             59..415
                     /locus_tag="ACHGNS_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFN6938619.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
     gene            405..671
                     /locus_tag="ACHGNS_002751"
     CDS             405..671
                     /locus_tag="ACHGNS_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN6938620.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            830..1831
                     /locus_tag="ACHGNS_002752"
     CDS             830..1831
                     /locus_tag="ACHGNS_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFN6938621.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(2201..2815)
                     /locus_tag="ACHGNS_002753"
                     /pseudo
     CDS             complement(2201..2815)
                     /locus_tag="ACHGNS_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            3055..3300
                     /locus_tag="ACHGNS_002754"
     CDS             3055..3300
                     /locus_tag="ACHGNS_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938622.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            3398..3619
                     /locus_tag="ACHGNS_002755"
     CDS             3398..3619
                     /locus_tag="ACHGNS_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938623.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            3941..4621
                     /locus_tag="ACHGNS_002756"
                     /pseudo
     CDS             3941..4621
                     /locus_tag="ACHGNS_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
     gene            4761..5300
                     /locus_tag="ACHGNS_002757"
     CDS             4761..5300
                     /locus_tag="ACHGNS_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938624.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
BASE COUNT         2213 a          822 c         1071 g         1899 t
ORIGIN      
        1 aattcgaatt tttatgttat atactataat ataaaatcaa tatatgatat aattgggtat
       61 ggaaaaaata tcttttgaaa catttaaaag acctaatggt catgatgaat ttttagaatg
      121 gttggaaaca ttaccaaaaa aagatagtgc gaagctttta cgtacaatag aagaaacaga
      181 aaagaatggg atgctagtag ctcaacggtt gaaatgggtg aaaaaattag acactaattt
      241 gtacgaatta agaagtaagg taggatcaaa catccaaaga gctatctatt tccatgtggt
      301 caatggaagg tatgtcatta cccatggatt tactaaaaaa acggagaaaa caccaataag
      361 tgaacttaaa catgcaaaag aacttagaga ggagtggttc acaaatgatg attaaaaaat
      421 tgattgaaga aaagcaaaaa gacaaagatt ttgcagaagc atatgaccgt gaaggaagaa
      481 aactcgaaac tgcggttgct ctttatcatg cacgggagga agcaggccta actcaagcgg
      541 agcttgcaca aaaagcacat acaactcaat ctactattgc tcgaattgag agaggggata
      601 acgtatcttt cgataaactt gatgatatcg ctagggcatt aggcaaaaag ctggtagtag
      661 aatttgctta aaagcgagtt gaaaatatac ctgtaatagg ctcctaacta atcaattagt
      721 ttagtgggcc tttttgtact aaatagataa aagttgtcct atagtgtaaa agtcaataaa
      781 ttttatacaa aaattacttt aaagagacat aaagagaaag gtacaataaa tgaaaaccat
      841 aaatctaata aaagtattta tagcatcacc aggtgatata gcagcaaaga ggaatgaagt
      901 agaagaaacc atatatagat ggaatataga aaatacagat aatacaaaag taattttgat
      961 gccaattcga tgggaaacga atgcgattcc tacttatata aataatagct ctgggcaaag
     1021 tgttattaat aaacaaattg tagaaaatag tgatatatta ttagcgattt ttggccataa
     1081 gttgggttct ccagttgata attatagatc gggaactata gcagaaattg attactttta
     1141 taaaaatagc aaaggcgagg taggggtttt tttcttagat gaaccagttc cacaagtttt
     1201 ggctgaagag tatatagaag ttaagaagta cagggactct ttagaagata agggttttta
     1261 tggaacatat agcaaagaca atatcagaag gtttctcaca aaaaaagtga attatttaat
     1321 tgagaaagca caaaataatt tagaatcaaa acgctttgat ttagattcta tttcttacaa
     1381 gacaccttca gtaattaaca aaaaagttac cggaactgat aaagattttg atgacaactt
     1441 agatattttt gatccaatag aatttgacaa tgatgaaaga ttgctgctaa tatatgcgtt
     1501 tatggatgaa aattctatct tttcaactga taattcaact tatagaaaag tagttcaatg
     1561 ggaaagtgat aacaatttaa ggagttactt gagtgataga gtatttgatg tactagaaaa
     1621 attaagtaaa tcgggaattt taaaggaaga atacgattct tcttttgatg agtatccatc
     1681 acgctatatt ttgatttcag aggactataa gaaatttaaa aggtattttt taaaaaatga
     1741 aaaaaagata tctgttatta tggagaaata caaagctcca atacaggctg atccattttc
     1801 caatttggat gaatcagatt taccgtttta aattttaaat agataatttc attttttagt
     1861 agcagaggta taaacttttt taatgtttga attgaaaaga aatgctaata ggtcgacttt
     1921 atgtttctct tgtgcaatcg taaatgcggg ggaccataaa attgatctat tatcgagtgt
     1981 gtataaaata aaattcaaat cttattagtt tacataagat acattatact aagttgtcaa
     2041 tgttaaatgc ctttaaataa ggacgattta tgcatcaatt atatttatgt taactaaatt
     2101 ctttttaaca taaatgtaat caacttagct agatgaatct aaaatgattg tctgtattga
     2161 tgacaaagct aattaataat tacgtgattc aaaaaagaaa tcatacaatt agttggtcga
     2221 tttctttttt tattcggtaa attgtttgcc tagtcacatt gtagtctgtt gcaatctttg
     2281 agatagcaac cccattctca agatcctgaa cgatactcct atagacaagg cgacgttgag
     2341 gatctttggt gtctgcactg tataacttag gacgaccttt atagacgccg tttgctttag
     2401 cgattttaat tccttgtgcc tggcgtcgtt tcgattcagt acgttcttgt tcagcaatca
     2461 tggctgagat ttaaatgatt aagtctttga tgaatttatc taggagagga tttccaattg
     2521 cttcagccat gatcggtaaa ctggtgataa taagtcgcac attcttcttc tttagataat
     2581 tcactgaatc gataatctcg tcataattgc gtccaagtcg atcaatggct tctacaataa
     2641 aaatatcttg ctctctgaca aaatctaaag cttcctggaa aatcggccga tgactaatcg
     2701 tcgctccaga ttgtttttcg ataaaaattt ttttagcgcc aaactttttc atttcttcaa
     2761 tttgacgttg ttcattttga tcgatggatg aaactctaat ataagcaatt ttcaaattta
     2821 ttccctactt ttctataacg tttataatac accctaatca tacgttagaa ctctaaattt
     2881 ttcaatgtta tatttttgtc tatttagggt aaactctaaa tagacaaacg gtttttgaat
     2941 tctaaaagtt aaatagttta taatagaaga aaaagaatgg ggtgacgatt tcttagattg
     3001 aaaaggaaag ataaatatta tagattatta cttttgacag ctgaagcagg atggatggat
     3061 tttccagaag ttgtaaaaga agttcaagag ataggtgcaa ctatttctga tagccgctcc
     3121 tctcctatga actatcagga tgcccatgga attaaaataa ttgatcaaga gagaaatgtc
     3181 agaaaataca caacaataaa tgagtgtgta attaatgaaa atctttgtag ggcaccgata
     3241 acgaaacaca ttaagaatag aagcaaagca aaagacggaa gaacttttac taccttctaa
     3301 gtttttttca tatttgtttg ctatacttag atctaaggaa ttagactttc gctgcccatt
     3361 atttaagttt gaggtagcgt cttgcgaggg atcaataatg gaagaagaaa aaatatttga
     3421 aaaacgctgg gaattggctt cagttgaaca aagagcaagg taccataatc taatgtcctc
     3481 ttaccgtaat attgattgga cttacaaaga aaagaagtac ttgctttggt tatgtcagtt
     3541 agatgtaaat acctttgaaa catttgaagt gattctagat aagattaaaa acagcaatga
     3601 aaaaagagct gatctataat ggttcgctct ttttatttta ttaactcttt ggttactata
     3661 tgtaaaatac agcttcatat tacaataaat ttcaggatac aaatttattt aagcatatat
     3721 aaatataatt gaaataggac ataaatatat gagtatgaga aaggtgattc catgtttaat
     3781 aaatgggcaa accacccaat gaactcgaga ggtagagata atttgaagga gacggacaag
     3841 ttctgggaag aagtatatag taaaaacggt tctgttgcaa agttttccaa aaaatctatt
     3901 ttagtgtaaa attgagaaaa aagacagaga ggacagagta atgaatcatt ttaaaggcaa
     3961 acaattcaaa aaagacgtca ttattgtcgc tgttggttac tacctgcgtt acaatctaag
     4021 ctatcgtgaa gttcaggaat tgttatatga tcgtggaata aatgtttgtc atactacgat
     4081 ttatcgttgg gtataagagt acagcaaagt cctctattat ctttggaaga agaaaaatag
     4141 acaatccttc tattcatgga aaatggacga aacctatatc aaaattaagg gacgttggca
     4201 ttatctttat cgtgcaattg atgcggacgg cttaacctta gatatctggt tacgaaagaa
     4261 acgggatacg caagcagcct atgctttctt aaaacgactc cataaacagt ttggtgagcc
     4321 gaaagcaatt gtgaccgata aagcaccttc tcttggctcc gcctttagaa agttacagag
     4381 tgtgggttta tatactaaga cagagcaccg aactgtgaag tatcttaaca atttaataga
     4441 acaagaccat cgacctatta aacgacgaaa taaattttat caaagtctcc gtacagcctc
     4501 ttccacgatt aagggcatgg agaccattcg aggaatatat aaaaagaacc gaagaaatgg
     4561 aacgctcttc ggcttttcgg tgtctactga aatcaaggta ttaatgggaa taacagccta
     4621 agatatttgg agttcagaga gggcgcgttt gattttcaaa cttcgcaaca gaaccgagaa
     4681 ccttgggttt tcaagtgctt tttggttcat taatcttaac catagtgttt ttaaagtgga
     4741 aaacaagcaa caaaaaaagt atgtggagcc ttgtaggaat agttcttatt atttttactg
     4801 tgactgcagc aacagaagca aattattcgc ataaaattca gtcgtataaa ggtcgtttag
     4861 ttatgaccaa aaaagatgtt acacgttata catcctcaag aactggaggc ctatatgact
     4921 atgcaccatt agatgcattg aaatataaag atcatttaaa gaaacatgaa gtaaaagttg
     4981 gtgatcggtg ggagaatgca tctcataaag catataattc caaaatagtc tatacagtag
     5041 aagatgccga tgagcaaaat atcacattac ctgtattcaa ttattgggga acagttgtga
     5101 aagtaaatgg gaaaaagagc actatagaaa atgacaatgg tttagtgtct attaaaggta
     5161 cggggaaaag aactatagtt gaggtttcat ctacgtatcc aactacgatg attctatctt
     5221 tattaattag ttcaataact tttatattct tatttacacg ttatttaata agatgctgcg
     5281 gtgaaatgaa aatagattag atagatatta taaatatgtt gattctattt tcatagcaat
     5341 ggagaaaata cattagcaat atataaaaga aggtatcaag agtcacagtg aatttattcc
     5401 gaaaagtgaa aagagcaaat ataaaattta atgaaaatat agaactaaat aggaaaacgt
     5461 agacgtaatt ttctacgttt tccttttcat atatattaaa tattacggaa aattatataa
     5521 actaatttag tgttttgttt gtaattccta attttgctcc acttttagat aatttttagg
     5581 tctatttaac tcatcagaca ttctatgctc atttttctcc aaatttgttt caactctttc
     5641 gtacttaatt ttgcgtattt gaaaattctt cttttagagt acttttatta cgatactagt
     5701 atgatatata tctatagcaa acagttaaaa tcttcctaaa ataagcgaat agcattccta
     5761 aaacctataa aatttttaga tgaagcgaca agtggacaat taataaacta cttgaacatg
     5821 aaattatgga tcacctttca aatttagaaa atggaattct tatacttatt gcgcaacatt
     5881 tattgattgc aaaattatgt gacaaaaaaa actagtagaa gaaagaataa tggcttgata
     5941 ataaagtact acctattaaa ggtagtactt tatttaattt agtataaatt aacatactac
     6001 tatat
//
LOCUS       DAZBTF010000120         5988 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000120 DAZBTF010000000
VERSION     DAZBTF010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.125"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..450)
                     /locus_tag="ACHGNS_002758"
     CDS             complement(<1..450)
                     /locus_tag="ACHGNS_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN6938625.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELITN"
     gene            674..1465
                     /locus_tag="ACHGNS_002759"
     CDS             674..1465
                     /locus_tag="ACHGNS_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFN6938626.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            1431..2384
                     /locus_tag="ACHGNS_002760"
     CDS             1431..2384
                     /locus_tag="ACHGNS_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HFN6938627.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(2782..3756)
                     /locus_tag="ACHGNS_002761"
     CDS             complement(2782..3756)
                     /locus_tag="ACHGNS_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN6938628.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3871..4305
                     /locus_tag="ACHGNS_002762"
     CDS             3871..4305
                     /locus_tag="ACHGNS_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFN6938629.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(4430..5452)
                     /locus_tag="ACHGNS_002763"
     CDS             complement(4430..5452)
                     /locus_tag="ACHGNS_002763"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFN6938630.1"
                     /translation="MKKIGFLSFGHWMEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGA
                     FFRVHHFAPQIGSPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQG
                     RVQLGVGRGSPEQVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPM
                     FPQGSGKLRIEPYSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQ
                     QANQLRAYKEAWKQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYH
                     RNPTIFGKTFAGEPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIA
                     PELGWR"
     gene            5844..>5988
                     /locus_tag="ACHGNS_002764"
     CDS             5844..>5988
                     /locus_tag="ACHGNS_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HFN6938631.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIRSAGIETHGLN
                     PRAI"
BASE COUNT         1884 a         1129 c         1031 g         1944 t
ORIGIN      
        1 atttgtaata agttcgtata tgttttgcgg tgtttcttgt ttcttgtaat tttccacgaa
       61 tgtttcatcc atgaataaat tcataaacgc ttgaagtaat cctacttgtt cttttggttc
      121 tttgattcct aaaaaaaata gattttctac agtcatttcc tgattatccc ccatttgata
      181 aaaataaatt gggttcttca gctgcataac aaaaacaaat ggcttttcga catattcaca
      241 ttccgaatgt ggcagcgcga tatttaaata cttagtaatc aatccagtag gaaattctct
      301 ttctcttgca atcagtccat tcaaataatt ctcattgacg taccccattt tttctaattg
      361 ttgtgcagcg attaaaaaag cttcttcttt attttttact tctaccgcta attgaatcaa
      421 actaggaacg aacatctctt tatatttcat tccaacctcc tcctttttga ttaagtttgt
      481 taatttattg ttctactaaa ttcatctttg ttttacaaga acattatatt tctgttagtt
      541 tgttttgtca acgctttcaa aagaaattta ttttgttaaa tttgatctac ttgaaaataa
      601 aatcgtttat ctatataatt aagaagaaca aagtcgaaca aaaattgact tttatgttaa
      661 aggagtaatt catatgctga aaaaagaaag acaagaacaa atcattacat tattagagaa
      721 agatttttat ttaactaatt cagaaatcgc taacaaatta ggcgtatcag aaatgaccat
      781 acgccgagat gtcactgaat tagcaaaaaa aaataaagta aaaaagctat atggtggcgt
      841 tgaacgaaag gaaccttata atcgagaact atcgactcaa gaaaagattg atacgaatgt
      901 agccgaaaaa aaatatattg gtcaagtcat gaacagtatg attcccgata attctactat
      961 ttatgtcggt gctggaacaa cgatcctata tgcacttcct ttcattcaaa agaaaaatct
     1021 atttgtaatt acaaacagtt tgattgcttt caactattta aagaatcata caagttatcg
     1081 gattttactt actggtggcg aattttctcc tattacagaa gagttcattg gggaagttgc
     1141 agaaagatct tttgaaagtt tgaatgtcga tatctctttc gctgcaacaa acggcatatt
     1201 tgataacaat gtgacaaccg ctcaatttat tgagggaaat attcagaacg cagcgctcaa
     1261 tgcagcaaaa atcaaatgca ttgttgcaga ttattcaaaa ttaaataaat cagacgtcta
     1321 tacttttaga aaattaaccg attttgatta tctaataacc gataatcatt tatcagaaca
     1381 ggatttcaat cattattcaa aatacttaac aatattaaag gagcccaaaa atgatactga
     1441 caatcacgat gaacccatcc attgattcca tttattttac aaaacacttt gaattaggta
     1501 cgatgacacg ctttgattct ccggtacgct atgtaggcgg caagggaatc aattgcggac
     1561 gagcttctgc tctactaggt tcatcagtta ttttaacagg gtttttgggc ggttattttg
     1621 gcagtatgat tcaagatatg ctgaaaaatg aaaatttgtt tgatttgcat tttttaccta
     1681 ttgcagaaga atcaagaggt gccgtcacga tcatgcatga tgatgaaacg cagacagagc
     1741 tagtggaagc tggtcctgaa atagcagata ctactatcaa aaaattaatg aatgatatca
     1801 aactaatcag taaaaataat tacctttcag tcattacaat aaatggttct gttaacaatc
     1861 cagatccctt tttgtatgct aaattattac aatatatcaa agaacaaata gatccttcca
     1921 cctatatatt aatggatata tctggacaac atctccgtga agtcttagca agtaagtata
     1981 aaccgatgtt catcaaacct aatatccatg agttttcaga tatcatccat caaaaaatta
     2041 ctcaaaaaag cgacataaaa caccatctaa aaacaaatcc actatttaaa gatatcgatg
     2101 ttatcatgat ttcttgcggc tcgtatggtg cagtagtcaa agtaaaagaa gaattttgga
     2161 atgttgagat ccctaagatt cctatcatta atacaacagg ctctggggat gccactgttg
     2221 gaggttttgc tcattcatta gaacagaatt atcctttaaa agaatgtgta aggtacgcaa
     2281 tggcttgcgg catgtcgaat gcacagcaat cagaagtcgg gaaagttatt ccagctgatg
     2341 taaaaaattt agcaaaaagc attaaaatca cagaaatgaa ataaatagta caaagtaaat
     2401 aattgtttat atgaaatgag acacaaagag actgtgacaa aaaacgtgtg taccgacccc
     2461 caaaagttag attttttggt ctaacttttg ggagtcgata cattgtcaca gtcctttttt
     2521 cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
     2581 gagaagctat tttttgaaat tgctccttgt gactcagagt taaacggcag gctcacgacc
     2641 tcttggtata aacggtgttc aaaagcttgc atctacgcta atgagtccaa ccaagtcaaa
     2701 aatgaggagc ttttttggct gtccttatta cttgatgcag ggcagttttt tcataatctc
     2761 ttttagatta aatccttttg gttacacaag gttctcttca aagaatgttg ccattttttc
     2821 aaatggaatg atgtctgtct ggtcgtatag atctgtatgg gatgctcctg gaatgatcat
     2881 gagttccttg ttgtcacctt ttagttcttt gaaagcgtcc tccccaaagt aacgtgaatg
     2941 agctttttcc ccgtgaatga ctaagactgc atgacggatt tctggaatat aatgcaatag
     3001 ttttgcattc ataagtgaca accctgatgt cctattccaa ccatcattgg agtttaaaga
     3061 acggacatga tatccgcggt ctgttttata atacgcatga tagtctttga caaattgagg
     3121 agcatcttca ggcaacggat caaccacacc tccagcacgt gcgtagtccc cttgtttgaa
     3181 atcaatgatg cgttgtgcat ttaattcttc acgcatttca tatcgggcat tttcatccat
     3241 aacatcgaag tagccatttg ctgttactcg agtcatgtca tacattgtgg aaacaactgt
     3301 tgctttgacc cgcgtatcga tagctgctgc attcaatgcc atacctcccc atccacaaat
     3361 accaatgatt ccgattctct cagggtttac atttttttgt acagataaaa agtctactgc
     3421 cgcttggaaa tcttccgtat tgatatcagg cgaagcaaca tatcgaggta ctcctccgct
     3481 ttctcctgta aacgatggat caaacgcaat cgttaaaaag cctctttcag ccatcgtctg
     3541 cgcatataga ccagacgatt gttccttcac tgcaccaaat ggcccgctca cagcgattgc
     3601 cggcaatttc ttttcactta cttcttttgg ttcatataaa tcagcgacta atgtgatacc
     3661 aaaacgattc gggaagctca cctttcgatg attgacttga ttgctttttg gaaatacttt
     3721 gtcccattct tgtgtgatgt ttaattcttt attcatattc tagactctcc tttgatcaaa
     3781 attgacattt gtttttgaat gtattattat gataaaactt aaacttgact ttaggtcaag
     3841 gtaaaaattt tattttagga ggattttttt atgtattcca ttggacaagt gtctgaaatg
     3901 tttggattgc ctgtttctac tttacgttat tacgacaaag agggattatt tcctgatatc
     3961 cagcgtgttt ctggtattag aaaattttca gagaatgagt tagaggcttt aagagtcatt
     4021 gactgtttga aaaaatctgg tttagaaata aaagacatta agcaattcat ggaatggagc
     4081 aaagaaggca gtaagacatt tgaggttcgt aaagaactat ttgaaaaaca aaaagaagta
     4141 gtagaaaaag aaatagcaaa attagaaaga gtactcgcta tgctaaacta caaaagctgg
     4201 tactatgaag aagcaataaa agccggcaat gaagaagccg tattgacaat gattcctgat
     4261 gatttacccc aacatgtaaa agaagcttat gtacattctc attgaacaaa cggataactc
     4321 actctccttc tctatgaact tcatgaaaac atgaataaac acctctaact gattgttaga
     4381 agtgtttatt ttttcactca agtatcctgc acttacaaaa atatcctttt taccgccatc
     4441 ctaattctgg agcgatatct tttacgattg tttcaagcaa gtgcatgtta tattctaccc
     4501 ccaaagtatt aggaatcgtg acaagtactg tgtctgcttc tttgattgct tcatcttcag
     4561 ctagctcctt gattaattga tcaggttcac cagcaaaagt ttttccaaaa atcgttgggt
     4621 ttcggtgata agcaagatag ccgacagcat cttgttgcgg actaccgtct tgtccaaata
     4681 agcgtcgatc caaatcagtg gtgattggct gaatcgagcg agtaactaat gttcttggtt
     4741 caaaatcatg acctgcttgc ttccacgctt ctttatacgc acgtaattgg tttgcttgtt
     4801 ggatatggaa tggctcgtgg gattcgtaat tgactaatgt agatgattgc agattcattc
     4861 catgttgtgc tgcccagatt gccgtttgct gcgaaccagc tccccaccag attctttgtc
     4921 gtaatccttc tgaataaggt tctatccgta attttcctga tccttgtggg aacattggtt
     4981 gaggatttgg ttcttcaaat ggttttcctt caagtaattc caagaattca agggtgcgtt
     5041 cccgtgttac ctctttgatt tcttcttcac tgtaatcata accaaagtat ttccagccat
     5101 ctagaacttg ttcaggtgaa ccacgcccca ctcctagttg cactcttcct tgcgtgatga
     5161 tatctgtcaa tccggcattt tctgccatat aataagggtt ttcatatcgc atatcaataa
     5221 gtcctgtacc gatttcaatc ttacttgtct ttgcgccgat tgccgctagc aaaggaaacg
     5281 gcgacccaat ctgaggggca aaatgatgta cgcggaaaaa cgctccgtcc actccgattt
     5341 tttctgcttc caccgctaaa tcaatcgatt gcaaataagc atcttgagct gatttcacaa
     5401 gcgatccttg ctccatccaa tgtccaaagc ttaaaaagcc gatttttttc aaggtaatga
     5461 cctcctttaa acctacaaaa atggaaggtt ttttattttt aatatgaact gattctacta
     5521 ctttttttct taaaagacga ataattgttc tcgcctcaca atcaagaaat gtatcggctt
     5581 actacacccc tactttcttc atcatctgcc gtgtcttaat ctcttttaac agtctgttac
     5641 atcgttgatt tttgactcaa aatgattgtt tctaacatgt atttatggta aaaaagacaa
     5701 gattagttct ttgtacacat cgatttatac gctgaacaaa aagaaatata taaactaaga
     5761 aacccattgt tttttcgctc catcacatag agtttacttt agataaacag tgacatttac
     5821 taatccataa cggaggaaat caaatgaaaa aaatctattt cttatgtacg ggcaattcat
     5881 gccgcagtca aatagcagaa ggctacgcgc gtaaatactt gtctccttca atgtttgaga
     5941 taagaagtgc ggggatcgag acacatggct tgaatccaag agctataa
//
LOCUS       DAZBTF010000121         5948 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000121 DAZBTF010000000
VERSION     DAZBTF010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.126"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..186
                     /locus_tag="ACHGNS_002765"
     CDS             <1..186
                     /locus_tag="ACHGNS_002765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938632.1"
                     /translation="HRKYRVSDFSTTSKNYCFFPLKCLQKVGSGRIYWQNQGEGSLNK
                     EKNHRKYKVITYKTSFL"
     gene            complement(473..976)
                     /locus_tag="ACHGNS_002766"
     CDS             complement(473..976)
                     /locus_tag="ACHGNS_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFN6938633.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1335..2360
                     /locus_tag="ACHGNS_002767"
     CDS             1335..2360
                     /locus_tag="ACHGNS_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFN6938634.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2683..3561
                     /locus_tag="ACHGNS_002768"
                     /pseudo
     CDS             2683..3561
                     /locus_tag="ACHGNS_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
     gene            4037..4153
                     /locus_tag="ACHGNS_002769"
     CDS             4037..4153
                     /locus_tag="ACHGNS_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFN6938635.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5081..5419
                     /locus_tag="ACHGNS_002770"
     CDS             5081..5419
                     /locus_tag="ACHGNS_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938636.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5513..5743
                     /locus_tag="ACHGNS_002771"
     CDS             5513..5743
                     /locus_tag="ACHGNS_002771"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFN6938637.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            5757..>5948
                     /locus_tag="ACHGNS_002772"
     CDS             5757..>5948
                     /locus_tag="ACHGNS_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938638.1"
                     /translation="MKNFDTYYVIVFSISLLLILLTLMQYKEYPEYFILTFVYLVSSY
                     TLLYKFRSSSRVSKVCFLIS"
BASE COUNT         2016 a          893 c         1089 g         1950 t
ORIGIN      
        1 cacagaaaat accgtgtctc agatttctct actacttcta aaaactactg tttttttcct
       61 ttgaaatgcc ttcaaaaagt aggatcaggc agaatatact ggcagaatca aggagaaggg
      121 agcctaaata aggagaaaaa ccatagaaaa tataaagtaa ttacttataa aacaagcttt
      181 ttatagaaaa ataatatacc tgagtagggt ataaattaag acggaattcc tttctagatt
      241 gagatattga ctaggaagaa tgttaagatt ttaagccttt aagctaaaaa aaaacccttt
      301 ttgttaaata aggggaatat agcctgtcag tttcaaactg caagtcgtcc tgctgcaata
      361 atatagtaga ttagagatga aaatagattt ttttaaacca atgactgaat aaaaagaccg
      421 tcatagatac acattggaat gtgtctatta cggtcttttt ttacgaacga ttttattgaa
      481 gacggacagt tcgggtcaaa aataaaaaag ctacgatcag aaataagaat aaaatgtatc
      541 ccaaaataat ggctagacgg tctaacttat ttgtcgatag atgtttgttt agaagttgat
      601 aggcccataa acctatcagt cctcctgttg tattagttat caaatcagtg atgtccgcta
      661 cgccaatacc gaataggaat tgaatcaatt ctgccaagaa gcttacaagg aaaataatcc
      721 ctaatttttt agaaaaagaa agtctcttga aattgacatt caaaagcacc ccaaaaggaa
      781 aaaagacaat caagttataa gtggtctcac gtaataccgt cgtattttca aaagaaaaag
      841 tagaaaaagg gacgagattg acattgctgt aagaataagt gagaaattgt ggaagattaa
      901 atgatagctt gaataaaatc aaccataata gaaagaataa ataaatccct aataaaaatt
      961 ttgatacata ccgcatagac atctcctcca tttaaaaagt ataccatcga aaaccgctga
     1021 aaaatcataa aaaagtatga agtttaagga ccaacgtagt tatcgtacga aaattagaag
     1081 cttttttttg ttgggaatcc ggtttcttta acaaagctaa gatagcgagt tgcttggaaa
     1141 agttgctatc ttttttctgt caaaacgact cggaatgttc gttttttgtt ctaatttctc
     1201 caagttgttt actttttgat actaagttta tgaaaaatca agaaatagtt gttatactag
     1261 gtaatgctga caaacgggat tggcatttca tcttggttca gctttttttc caattactag
     1321 gaaggaggga tagattgttc aagacttatt ttcgtttcaa taaaatacaa gataatcctt
     1381 ttcgtatcat gaatctgatg ctacttatct ttattgtatt atggttaggt tacataaatc
     1441 atgatttttt gatttcaagt ttttcatcat tagggatctt tacttttttc tattatcaaa
     1501 acataccgat gaaatatttg ctgaaacgta tgtttctgat cggttgcgga ctgttggttg
     1561 ctttcgtttt ggggatctta tcgacttatg ttttatggct tgaaccattt gccgtagctt
     1621 tggttgcttt ttccagcaga ttcgtgttgc gattattcca tatttctaaa ccaggtggct
     1681 tgttttttgc gatgctttct gccatgggaa cgagcatgca gctgccaatc gctcaattac
     1741 cgatcgtctc actttatttt tttatgggtg tcgtttttgc cctgattgcg gcagtgatta
     1801 caaaattact ggattcacgg cctgagcaga caattgaaaa agcaacttta aaagaaagat
     1861 ttcacgaaga gcctttagtg atcattgatt ctgttttcta cagcgctgcc ttatttcttt
     1921 ccgtttatgt cagtcacggc ttgaatctgc ataatcctta ttggttgacc ttgtcatgtg
     1981 catcgatttt gctagcagaa aacttggatg cgatgaagca tcgacaagtc caatatctga
     2041 ttggatcgat gggtgggtta tgtgtgtcgg cattcttgtc atttgtgccg tttacgcagt
     2101 tgcagaccat ttttctgatc acttttctat atggaatcgc acaatttcta gttgctagaa
     2161 attatgcagt tgctaatatt tttcttaatc cgatggcgtt gatgctatct acacttattc
     2221 gtggagcata cttgatttct ttgatcgaat atcgtttttt agggatcgtc ataggaagtt
     2281 tcatcggttt aggtgttgcg tgggtaatga cggttggttt gcaacattat ttaacggttg
     2341 ttcgtcaaaa attagaatag aaggacaaat atatgtgcca aaagaggtta tgacaagatt
     2401 tttgtcacaa cctctaattc aaggtgttca aaagccagat tctcagcatt aagtcaaatt
     2461 cacacaaaaa tgtggaaagg aactttcaaa aaaattttat atttattcta tcaaaaaatt
     2521 ttgtttatga tggtaactga ataaactttc ctgagatggt caatgctttc cttgaaagtc
     2581 ttacagatat tctttcttca tagaaagttt aagttttata gttagtgtac aataaaagta
     2641 tgtttatacc atatggtgtc cggataatca gtcatttaaa caatgaggtt caaagatatg
     2701 aaaaggggaa atttgatgaa gaaaataagc aatgaaatcc aaattctggc agaaacttta
     2761 actcttccca aagtcgttat agagcaaatg attgtacaag caaaaaacat ccttgatgag
     2821 gtgtttgagc aatatagtca tgaactaatg gatcctaata aagcaaaggc gactgcaaag
     2881 aaaatagaca aagtctatga agaggaacct tttgcagcat tagctattta tctatatgca
     2941 gcttctcaaa gttggaaaaa aatttatcaa ccattgaaga ttccaagaac tatctatttg
     3001 gcaacaatga atgcttttac tagatttatt catgaacatt tccaagtaac aggaagctat
     3061 cgctttgacc gtggtttttg gatttggcat tacactagtg gcttgatctt tcggattggt
     3121 gaattggagt acgaaatgac atattttccg aaagggtaca cggatctaag attagaagga
     3181 aagcagtgcc tttctcttca tattccttcc gatgcagatt tatctcagaa taagataaga
     3241 gatagttacg aaagtgccat tcgttttttt gaactttatt ttcctgatta ccattaccaa
     3301 gtgatgtaca cagatacttg gttactatca cccaatctta ctaaatagtt gaaaaaagag
     3361 tcaaaaatct gtctttttgc agccgattat cgattattat cagttgatga acaggacgat
     3421 agtggagtcc cttggatctt tggacgagta gatgcccaaa tacatgatta tccagaaagt
     3481 acgagtttac agcgacaagc aaaagagcaa ttgcttgcag gcgagcacat tggctctggc
     3541 ttaggtattc ttacttcata agtttagtac ttcgaaatag tttaaaagag cgtgagacaa
     3601 aagtaaagaa cacttttgtc tcacgctcta aacacgtata aacggcggaa gcagaagcaa
     3661 ctccttcgga aataagctga aattcacaaa aatttgaaaa gcaattttcg tgaatttctt
     3721 cttatttctt ggagttaaac gcttctgtcc cagcctcttt tttagtgttc tatcgaacac
     3781 acagtacgtt caataggtaa actagctata acaatagata ttaaagttaa cggacaagag
     3841 ggcctttttt aggtagctaa aacgtaataa tgaaaattta tttccagaga taggagtcaa
     3901 tcagctagtc tgatcgactg ttttaacaag ttgactttca ttttaacttt tgataccatt
     3961 taaaacacaa atcgtaattg tttcgatttg tgttttttag catgactaca atcaaagaag
     4021 aaagtgggtc gtccatatgg aaaaaacaga tatctcaagt gcataccgcc gcttgaaaag
     4081 cccaaatatc aaaacacgaa aacgtgcgct gaagatcatc aaagatgtga aacggaatag
     4141 tgggaaaagg taagaagaag gaaacttaat taaaaaaata gccgaaaaac gtttttcggc
     4201 tattttcaat aatcggtttt tgtgtaaggt ttccgaaaga actgctgttg attttccatt
     4261 tttaacatat gataaattgg tagaatcttt cttttcaaat tgataccatt ccctaattcc
     4321 agttatatag ttaactacag aaccacaaag cacgtacgtt tataaaagta ttatctgtag
     4381 ttatttttca attccctttc aacctcttat tttgaccagt agtattgatc actttacctt
     4441 ttaacacctt tgttcgtttt tgaaacattt ttagtcaaga atggaaacgt aactacataa
     4501 tcaaaggttt tgcgaaatat ttcatgaaat atttcatgta gaagcgagca atacggttgc
     4561 aaataaccat tatcgtttaa taagcagtta cgaaagctcc caatatctct aacgttcagc
     4621 tcactccatt tcttttcttc aaaaaaatca gtgattcgta tctctttcta tttgtcgaaa
     4681 aagttaactt taaaaagtaa cgccgaaaaa atatgcaaaa aaataaaaaa gcagttacta
     4741 ttgctagaat aacatcaagg aataataagt tttggttttc agataatagt cgataagttg
     4801 tgtattggaa ttttagatac agtaataaag tatttttcat gaaattttgt gagaatcact
     4861 attacaagta tctttaataa aattaatggt taaggaggaa cttaaagtag aatgtaaatg
     4921 aatagttgta gtcagtcgat tgtgatacta gactcaaagt tgcaggaata agtttttcaa
     4981 tttgtgattg gaagataaaa aatgaaaaag aagggctttt ttttatactt tgttgattgt
     5041 agtgtttatt ttccctctgt ttagccaagt aacattttct atgactcaac cagtttcaag
     5101 tgaggaacat gataaccttt ttcgtgctac agaagagttt aagaaatatc aggaaaatat
     5161 tgttccggag actccattag ctagtgagcc aattactgat attgatggaa accaaatagg
     5221 atggtatata cagtacgaag ttatggtggt aaataatgac tcaatagtaa aaaatacagt
     5281 aggcttagag ctaacaccga ttgaaaaggt gagttcattg ttaacgtata tttatactga
     5341 tgaaagtgac gagactgaga tcattttaac ggattactca aaaattgcta ctgataaaaa
     5401 gtatatattg tggatgtaga cactagtgaa atactttatg atatagaaat tgatgaaaat
     5461 ttactagatg tagcacaaga aatcgatcat caaaagcagg aagctattcg atatgctggt
     5521 agaaaaggga aaagaaaata tttattacgt gaatgtagca aaagttagag aagatgaaaa
     5581 tgagtggaaa gaattcaaga gtcgctactc aattaactcg actccgacat ttacagttta
     5641 cagagaagga tcgatagaaa aaactgtttt ttggactaaa gaaagtggaa tgtctttagc
     5701 tgaagtagaa gagtttttgg attatgttag tatgcaacag taagggagtt cttattatga
     5761 aaaattttga cacatattat gttattgttt tttcgatatc tttgctgtta atattactaa
     5821 ctttaatgca gtataaagag tatccagagt attttatttt aacttttgta tatttagtaa
     5881 gtagctacac acttctatat aaattcagaa gttcttctag agtttctaaa gtttgtttct
     5941 taattagc
//
LOCUS       DAZBTF010000122         5727 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000122 DAZBTF010000000
VERSION     DAZBTF010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.127"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            196..921
                     /locus_tag="ACHGNS_002773"
     CDS             196..921
                     /locus_tag="ACHGNS_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938639.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGFSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            911..1420
                     /locus_tag="ACHGNS_002774"
     CDS             911..1420
                     /locus_tag="ACHGNS_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938640.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1490..2938)
                     /locus_tag="ACHGNS_002775"
     CDS             complement(1490..2938)
                     /locus_tag="ACHGNS_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN6938641.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2938..3654)
                     /locus_tag="ACHGNS_002776"
     CDS             complement(2938..3654)
                     /locus_tag="ACHGNS_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFN6938642.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3635..3985)
                     /locus_tag="ACHGNS_002777"
     CDS             complement(3635..3985)
                     /locus_tag="ACHGNS_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFN6938643.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            4129..4902
                     /locus_tag="ACHGNS_002778"
     CDS             4129..4902
                     /locus_tag="ACHGNS_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN6938644.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            5073..5439
                     /gene="ssrA"
                     /locus_tag="ACHGNS_002779"
     tmRNA           5073..5439
                     /gene="ssrA"
                     /locus_tag="ACHGNS_002779"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1662 a         1123 c          994 g         1948 t
ORIGIN      
        1 aaaattattg tcgctgctcc tcttttcgaa taaacagtgt tcaaaagctt gcatctacgg
       61 taatagaata gggcagcttc ttcacaacct cttgcttttt acaaaatatt ttctggtttt
      121 aattcgtgat ttcactaaag gaatttcata ctttattcat attcattttc tatacttttt
      181 atagaggtga aaagtatgaa aataatccgc tatgctactg ctcgattaat ttattacaaa
      241 aagcagacaa tactctattg tttattttca agcttttctg cttttttatt gatagcatgc
      301 ctgactcttt accattcgca atcggcttta gccaatcaag tccaagatcg gctttcattt
      361 attggcgctt ctgcatctac agagcttctt ccggctttag aattagctag ccgattttat
      421 ataaaaggca tattcagttt attttttcta tttgccttcc tttttatctt tttcttttac
      481 tattcagtca ggcaaaacaa gcaggaattg atcaattggc gcctgactgg tttttctaaa
      541 accaaattat tcctttttat tttctggcag ctgttctttc ctctctttct ttgttgtatc
      601 ttggttttat tgtggatcgt cagcttccag tcagtttatg aagcgcttct tcaaaggatc
      661 aattttcttg tcttaaagac tactgaactg ccagatatcc gtgatatctt gacttcggta
      721 agcggactta caattccgat ggatcaacag acttttttta ccatcgattt ccaaaatgat
      781 ctttttttca cagatatact taagggattt gcccaaactc ttattgggct cagtggatgc
      841 tgtctcattc ttagtctatt ccctttcact ttttttatcc atcatttgca aagtaggagg
      901 cctggacgta atgaaacctg aatatttgat tcatgctgaa cacttgaccg ttattttgtg
      961 tcctctcgaa aaacagcata aaatattgga gcacttgaaa aaagaggttc ttgttgcaaa
     1021 actggataat tttgcgatca tccaaaaaaa ttggccgatg tttccttatc tgaatcttaa
     1081 agaccatgtc ctgttggatg taccggaaaa agaaatcaaa caagatcgtt tggcctatca
     1141 agaaaaactt gatatctctc cttccttgct taattgtgcg gcagctgagt tgacgtcttt
     1201 tgaaaaagta aagctgcaat tacttcatgc cttgctttcc aagcgaaaca atattgttat
     1261 tgaagataca tttgatgaac ttagtgtatt ggagatccaa gagctactcc acttgctttc
     1321 ttatttagct catgaagaaa atcaaggtat cttactcttt actcatgatg ctactattgc
     1381 tcaaagtcct tatattgatc gtttagagcc cgcgggctaa atcaatcatt ttatagtaaa
     1441 aacaagcggt atagaagatc gattcatgat cttttatacc gcttgttttt tactcttttt
     1501 tgatttctac gtcaggcaaa cgaacagcga attttgttcc ttttcctagt tcgctttcta
     1561 cttctaattt tcctttatgc aagcgtacaa gttgctgcac gattggtaaa cctaatcctg
     1621 attcaccata ttttgtattt tttcttgaag gatctacttt ataataacga tcccagatac
     1681 ttttcatttg ttgttcagac attccaattc ccgtatcttc aatcgtgatg atcgtttcaa
     1741 gatagccttt ttctaaagta atcgtgattt ggccgttttc agtaaactgt atcgcgtttt
     1801 gaataatatt gaccatgatt tggacaaatc ggtcataatc agcgtaaaca tcaatatcat
     1861 caacggcttt caatgtcaat gtatctcctg ctgcttctgc tttagcttct aactgtgcaa
     1921 caatattttt caatgtttct gttccattga attttttaat gaccatactg atttgattcg
     1981 tacggatttt ttcataatcc aagttttcat tcaccaaacg gatcagacgc tctgtttcat
     2041 ttttcatcaa tttaatcgcg ttttctcgct gattttcagg gattgcattg tattctaacc
     2101 cttccagaag tccgttgatt gtcgtaagag gtgttctcat ctcgtgagaa gcatctgcca
     2161 taaattgtct tcgacgattt tcttgttctt caatttctgc ttgagattct ttcaatgaat
     2221 tcgtcatttt attgaaatca tctgctaact catcaaattc atctttgtca tgtacgggga
     2281 gctgtacatc aaaattacca ttcgtcactt ctttcgtcgc attccttaac cgattgatac
     2341 gcttaacttg gaatgctgca aaagcatagc tggcaataac tgcaacgata ctggaaaaaa
     2401 taaatccttt gaacaggttc agtgtaattg gacgaacgct gttatcgata tttcgcgcag
     2461 gttgactaac tacaagtgct ccatagaatt catgattcaa attaaatggc acaagtgcgt
     2521 aagaggtcgc ctgtgcttgt cctaaaatat ttttattcga tgtgaatttt tcttgacgtc
     2581 cattcctcag atttttccat tgatcagctg ttaaggtaaa attcagtaaa gctgtattag
     2641 tcggataaat gacacgttcg tttttatcga taaatataaa gttcacattt tgttctgtca
     2701 gtacttgctc tgtcagcagc aatgcatttt gaaacgcctg atcttgacta aactgcggga
     2761 gcgtatcggc ataggtttgc gttgtctttt caactgattc agcatatcca aacagttgct
     2821 ggtaattgtt ttcttcaatc gtttgttttg tcagttgtgt aaaagaaact ccaacgatca
     2881 ataagatcaa cgcgaccatt ccgcaaaaag ctaagagttg ctgatacaaa taacgcatta
     2941 ggcaacacca gaatcatcga atttgtagcc gactccccaa acagtttgga tgacttgtgg
     3001 tccgaccttt tcgatttttt gtcgtaattt cttgatatgg gcgtccactg tccgttcatc
     3061 accaaagtac tgataatccc agactaattc aagcaactgc tcccgagaga atacttgacg
     3121 tggttttttt gctaaagtaa acaatagatc gaactcttta ggcgtcagtc cttcaattgg
     3181 ccggttgtcc agatacgctt ctcgtgtttt cgtattcatc ttgaaatgac tagtctcgat
     3241 atcaaaatga tcatctgaaa ctgctgcttc ttctcttgct tctgccaact cactacggcg
     3301 atgcaaagct ttcattcgag caataagcgt cagaggactg aaaggttttg tgacataatc
     3361 atctgccccc atctctaagc cgatcacctg atcgctttca gaatctctag ctgtcagcat
     3421 gataatcggc acagtacttg aaactttgcg gatctctctt cctactagca taccgtccag
     3481 tgtgggcaaa ttcaggtcta gtgtaatcat atcccaatgt tgtgggtttt ctaaaaaggc
     3541 atcgagccct tcttttccat catacttgaa agttgcttcc caaccttcat ttaagaaaaa
     3601 catctgcatc atttcagaaa cagattgatt atcttcaatc attaaaatat tcattatcgt
     3661 tccctccagt ttccaggtca tatttctgta atttcttttc tgtcataata aaaataggca
     3721 taattggaaa tactagcaga agcatttcaa tcagaaaata attcgtatgc ccagatgtct
     3781 ttaaataata gccgatcaat gccatcagta agcccataag aaaaaaccag tttccgcttg
     3841 tcttttgagc cagccgccaa tgagcgtctg tttttgcggc aagaggcgaa tgatacccat
     3901 aaaaaggcag atttcctttt gagggcagaa tccgaaaaag actgccaatt attaagaata
     3961 agataccaat actgaaaaaa atcattcttt ccctcctaaa ttctcttctt atagtatact
     4021 ctaaattgtt gactgaacca atatgttcag gaattcttca cgaaaggttt gtcttttgtt
     4081 cattctttgt taagataaac ttattccaat aaaaaaggag ttttatttgt gtcaaattat
     4141 cgtgcaatca ctttctttga tcttgatgga acattactag atgcgaaatc aaaaatcaca
     4201 cccgaagtgg caaaagctat gaacgcatta aaagaaaaca atgttctccc cgtcatcgct
     4261 acaggacgta cagaagcaga aatccactct atcatggaag atgcgggcat tacttctgcg
     4321 gtcaccatga atggttcttt cattcgagta gacggcgaag aaatctattc tcaaacattt
     4381 tcttcagaag aatgtcaaaa aatgtatgat catgtacagt tgaacggtca tgaactttct
     4441 tactataacg atcagaaaat ctggtgtacc ggtcatagcc aaacaatgat caatgcttac
     4501 gacttcattc attcagatgt tccggaaatt gatcctttag gctttaaaga aaacacagta
     4561 aatatgttgc tgatcttatc tcaatcaggt gacgaatact atcacgaaca ttttcctgag
     4621 ctaacttttt atcggaatgg tccttactcg atcgatacag tgaagaaagg aatctcaaaa
     4681 ggggcaggtg tcaaacgtct gcaaaaagaa cttcagctgg aaaaagttcc tacttttgga
     4741 tttggcgatg gtccgaatga ttttgccttg ttggaagctt gcgacaacaa aatcgctatg
     4801 gacaacgcct atgacggatt aaaagaactt tctaccttta tcacaaagaa aaatacagaa
     4861 ggcggaattg tccacgcatt gaagtatttt gatctgattt aagatctttt tttgaagcta
     4921 cctcttttct taagaaaatc gcttattttt tcttaagaaa ggggcagtta ttatataaat
     4981 aatgggatat gattcgcttt tttattgaat ttatcccaat atatgctata ctaattttgt
     5041 atgaaaaata cacgtaaccc ttgttagcta acggggacgt tacggattcg acaggcacag
     5101 tcgagcttga attgcgtttc gtaggttacg tctacgtaaa aacgttacag ttaaatataa
     5161 ctgctaaaaa cgaaaacaac tcttacgctt tagctgccta aaaacagtta gcgtagatcc
     5221 tctcggcatc gcccatgtgc tcgagtaagg gtcctaactt tagtgggata cgtttcaact
     5281 ttccgtctgt aagttgaaaa agagaacatc agactagcga tacagaatgc ctgtcactcg
     5341 gcaagctgta aagtgaatcc ttaaatgagt tgactatgaa cgtagatttt taagtggcga
     5401 tgtgtttgga cgcgggttcg actcccgccg tctccatata tgtatggttg agacagataa
     5461 acatgtatga aaaacgttgt taaatcaacg tttttttatt ttggtcaagg agagaaaagt
     5521 agagaaatac aaaatttttt gcattgattt tgcattcaaa aaaacattca aacatttaga
     5581 aatccccaat acgtattcag gaaaatctat tttaaggttt acaaaaagaa cttttgttcg
     5641 tacactcttt ttgaggtgaa cttttatgct gatggaagga aaaacacaat tatggtttaa
     5701 gcatgttcct tcaaataagt tttttta
//
LOCUS       DAZBTF010000123         5539 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000123 DAZBTF010000000
VERSION     DAZBTF010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.128"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            289..903
                     /locus_tag="ACHGNS_002780"
     CDS             289..903
                     /locus_tag="ACHGNS_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN6938645.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(1068..1310)
                     /locus_tag="ACHGNS_002781"
                     /pseudo
     CDS             complement(1068..1310)
                     /locus_tag="ACHGNS_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1514..2833)
                     /locus_tag="ACHGNS_002782"
     CDS             complement(1514..2833)
                     /locus_tag="ACHGNS_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFN6938646.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            complement(2967..3575)
                     /locus_tag="ACHGNS_002783"
     CDS             complement(2967..3575)
                     /locus_tag="ACHGNS_002783"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938647.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSLNNSYF"
     gene            complement(4213..4470)
                     /locus_tag="ACHGNS_002784"
     CDS             complement(4213..4470)
                     /locus_tag="ACHGNS_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFN6938648.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(4463..4732)
                     /locus_tag="ACHGNS_002785"
     CDS             complement(4463..4732)
                     /locus_tag="ACHGNS_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFN6938649.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(4863..5042)
                     /locus_tag="ACHGNS_002786"
     CDS             complement(4863..5042)
                     /locus_tag="ACHGNS_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938650.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            5140..5376
                     /locus_tag="ACHGNS_002787"
     CDS             5140..5376
                     /locus_tag="ACHGNS_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938651.1"
                     /translation="MKQNTSIAKNTTKFKCTAAAWRISISPTNRTNNRGMILFIWISF
                     FLLGYRVLPAYFAPPKRRGRLERLVRNKNHSLSY"
BASE COUNT         1748 a          996 c          874 g         1921 t
ORIGIN      
        1 aatctaataa aaactctcac tctaaaagag tgagagactg caaattagag tacggacttt
       61 tttggagttc cgttctttaa tattaacaaa tgaataaaat ccttacaaca tttgagaata
      121 agttatcagt agacacttac aaaaaattct catactattt cctccatcat tagtttttgt
      181 ctatataagg tttaccctaa atagacactg gtataacatt gaaaaatatg gcttcctcgt
      241 gtatgattag ggtgtatttt aaatagttgc agaaagtagg taaaagtttt gagagttgca
      301 tacatacgtg tctcatcgat cgatcaaaat gaacaacgcc agattgaaga aatgaaaaaa
      361 ttcggtgcag agagaatttt catcgagaaa caatctggcg caacgattac tcatcgacct
      421 gttttccaag aagcattaga ttacgtccga gatcaagata tttttattgt ggaagctatt
      481 gatcgacttg gaagaaatta tgacgagatc atcgcttcag tcaattactt gaagaagaaa
      541 aatgtgaagc tgatcattac tagtttaccg atcatggctg aagcaattgg caatcctctc
      601 ctggataaat tcattaagga tctgatcatc caaattttgg ccatgatcgc tgaacaagaa
      661 cggactgaat cgaaacgtcg ccaggcacaa ggaattaaga tcgcaaaagc aaacggcgtc
      721 tacaaagggc ggcccaaatt atacagtgca gacgcaaaag atccccaacg tcgccttgtt
      781 tacaaaagca tcgttgaaga tcttaaaaat ggtgttgcga ttgctaagat tgctaaagac
      841 tacaatgtta cgagacaaac cgtgtataga ataaaaaaag acagcatggt caatgacaaa
      901 tagttgatca tgctgctttt attatcttct catctttaat ttcctacata taattactat
      961 ctataaaatt aagtcttact cagaacagat aaagcataaa attccaacaa accatttttt
     1021 acttaactaa ataaagagtc tgttaatata atcttcactt atttacgtta atcccctact
     1081 gtatttgttg gttccttatc tatattttct acaaaatcct tattatcttg tgatttagct
     1141 tcgtataatt ctttgtatgt cgacacattg gttatactaa ttcctatagc tagttcttga
     1201 gactctactt ttctctctag ctcctttgac aaagtgattt gagatttatt tggttccgtt
     1261 ggagtcaatt ctatagattc ttcataaaaa tctctataca taaagctatc cctgaataat
     1321 tcatagcttt aattctttgg cacattttat tgaaatatgt atcactaata tccctatcaa
     1381 ctgattttga ctaaaaagtt acttcaaaca ttcactgttc aaaaatattg aggaataaat
     1441 tttgggtgta aggcattttg agcatacttc tccctggata acttcccagt aaaaaaatcg
     1501 ttagattgtt ggattagatc cattgactgg ctcgcctcag gcgagcaaag accctgtaga
     1561 attcattctg atacacatga taactagata atttgagata gactcgtcta cccgtttgga
     1621 ctaatttccc agcaactttc agaaacttca atcgaataga atgaatggtc attccctttt
     1681 ggacttcttc aaagccaatc gttcttaaga agttgactaa gttgtaagct atcaagctga
     1741 gcatcattcg gacatgattc tccaaaaagc gaggactgtc tgttttgtca aagtaaaagc
     1801 cagctttcgc ttctttaatg aagttctcca ttgtgccacg tttggcatag atagaaaaga
     1861 tggtatcagg agaaacattt tctgatagat tcgtcacgat aaattcatgt cgaaagagta
     1921 gttcgcccgc ttcacgtgtt gagcgtatac acacgcgacg actttgtgac caggattgtg
     1981 cttgataagt ggtcgagaag tactgaactt ctcgttcttc ccacttttga ttatcgccat
     2041 aaagtactga tttctcagct atttgaccta gtctacgatt attcttcagt cgaataacat
     2101 aatgactctt ttttgattca cacgaatcat acacaccagg tgtagcgaac ccgctgtctc
     2161 cacgaaccaa gatgtcagtg tttggtagag agtgattata gtgctctaat aaaggtgtga
     2221 gaaactcctt tacgcctttt gatgtatatt gatttcctga acgtagttca gcttttaaga
     2281 agtcaccagt caatccgtca aaagctacca atggatggta accataggtt tggtagtgtg
     2341 tattataatt cgtttgttct tggtgaccaa atgtatctga atgggtcgaa tctaaatcaa
     2401 tgattaattc cgtatcatta cggatgaggc gtgctttatc gataagttct tggttcaaag
     2461 cttgaagttc atggatattc tcctcagata gtcgatctaa aaatcgggaa agtgaagatt
     2521 gagaagcgag ttctttccta tctaaaacag ctttaaacac aggatcttgt ctcaggagat
     2581 tagctgcaga atcagctgag taaccagcaa ttaattgcat aatgaactgc tcaagtatgg
     2641 ataagttgtc atgagtaaaa tacgctcgtt cgtcttcaat gtgaatgtgt tgcttagcca
     2701 gttcagaaaa accaaagatg ttcatcagct ctttcactag gacgagaccc gaatcactcg
     2761 ataattgacc acctgtatga gaaatagtga tatttgaatt gaattttact tggtttttgt
     2821 gtaagctagt catgaaaaga actcctttct tttggttggt ttagtcactt taaccatagc
     2881 agaaaggggt tcttttttca tcacttaacg ggtgaagatg aaaaagtaat tgtaagctgc
     2941 cgtgaggcag tttcacatgg ttttaattaa aagtatgaat tattcaggct atcattaatt
     3001 ttataaaaaa aattaaatgt ttttagcact gccatataac attcaaaaca ttcttctata
     3061 gttggtttac ttccttgagg agaatgaacc aatttattac gtttttctat gaaattttta
     3121 atttcctttc ttatttcttt cggaaactca atattaaagt accctttaaa catgctccct
     3181 aataatttgt ctacaggggg agattgtaaa ttctcaaata ttagagttaa atcaggtttc
     3241 tcttgttgat aaaattcctt tacccctatt tcacaggctg taattatcga aataaaccta
     3301 tctctagtat ttgatatatt tttacttctt ttaaggagta taagagaaaa ggggatactt
     3361 tcgtgtccat gcatgtaatt aactaattgt tgagcttcag aagttatatc tctaattata
     3421 tagttaacgg aattaggaag tgtctttaac tctaatgaaa tttctatcca attactatct
     3481 tctaatctgt atatgctcga agtataattt gattcgtcag caagcatcgc tacatcccaa
     3541 acactgatca taaaatcttt taaacgttca atcatttcag caatattgct aaaatcagct
     3601 aatttttctt tagaataaaa aaaaacattt atatcagaat caataaattc tattttttta
     3661 ttactatatc gtcatcatta tcttgaccat ttatgtttat tgttccctga gtggggagta
     3721 cttctttcaa attcaagtta aatttatatt ccattacttc tcctccaata tataaaaatt
     3781 acttaactgt atccaaattt aatatgtaaa taaatcatta atttttagct aaattctatg
     3841 agaagtttat ttttgttgtt tttgctaatt aactaaaact agtacaaaaa atttaaaata
     3901 gattattaaa cctattgctt attctattca gtcatatata taagcgtcat cctttagtaa
     3961 ctaacatccc acactacaat agtattataa catatctatt caacttcaaa ttgttgaaat
     4021 ttaagccatt tatatttact tttcctcaac tttagaaatg attttactaa ccttatatcc
     4081 gtaatacgcg taacaaacac attcattaat aacaatcaac actaagctat attagctttt
     4141 taaatgacta gggtaaataa aataaggatc aaaaactttt aagtttctga ccctttcctt
     4201 acttccgatt ggttaatagt gatcttttgc agaataaata aatatcgttt cattttcaac
     4261 tctatatatc agtctatgtt catctgtaat tcttctggac cattttccag ataaatcatg
     4321 ctttaatggc tcaggttttc ctaatccagc aaagggggaa cgatcgatat cttttattaa
     4381 cttgttaatc ttttttatat tgcttttgtt tccttgctca tgccaataaa gataatcatc
     4441 ccaagcatca tcagaccaag ccttaatcat cagattcaac ctcgattaag tcatgtgttt
     4501 taaatgcacc tttggagaat tgttcatctc ctcgacgaat tttttccatg acgtaattat
     4561 tagaaagtgt tctcaacgtt tcttgcatag aatcataatc tctttttgat aatacaacaa
     4621 ctgtatcttc tacatcttta cttgttacaa taagtgtttc agcatcctca ttaacttgtt
     4681 tcatataact acgtaaattt tggcggaaat ttgaataagc tactgcttcc attcctttca
     4741 ccccttattt atttctataa aacaattgta cattatattg tacaattaag caaatgatta
     4801 atattctaac ttcttataat taaaagtgct atcactttta gtttttagga taaaaagtga
     4861 cgtcactttt tgagtgctgg tacctcaaat cctgcttttt cttgggcaat caataaatct
     4921 aacgcaatac acataatcct ggattcactt ttgccgtact tctgctttag ttgctgcaat
     4981 tctttttgtc tcttttcatc caacgtaacg ggtaaccgtt tatttcctat tgcaattggc
     5041 attagtactc tctcctttca tcgaaataac cctatgaaaa atatggctgc aatcatcttg
     5101 aatagtagac tcacaagggc taatacaaag cgccaaaaga tcaaacaaaa taccagtatt
     5161 gccaaaaata ctacgaagtt taaatgcaca gctgcagcat ggcgcataag tatttcgcca
     5221 acaaatagga ctaacaatag aggaatgatt ttattcatat ggatctcctt ctttctctta
     5281 ggttatcggg tgttgcccgc ttatttcgca ccacctaagc ggcgggggcg gttggagcga
     5341 ttggtgcgaa ataagaacca ttcccttagt tactaggttt tttgtcctta tttcacacat
     5401 tctcactttt cggtataaat gattattgcc tttttttcct ttcaaatcgt tgatatggca
     5461 ttcgtttacg gtcaagttca ttttggattt cttctgtatc gtcgtaagta taggtgtcta
     5521 aaaaatcagt taaagtcgt
//
LOCUS       DAZBTF010000124         5208 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000124 DAZBTF010000000
VERSION     DAZBTF010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.130"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..321
                     /locus_tag="ACHGNS_002788"
                     /pseudo
     CDS             1..321
                     /locus_tag="ACHGNS_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324676.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(593..883)
                     /locus_tag="ACHGNS_002789"
     CDS             complement(593..883)
                     /locus_tag="ACHGNS_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938652.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1285..1521
                     /locus_tag="ACHGNS_002790"
     CDS             1285..1521
                     /locus_tag="ACHGNS_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938653.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1528..3480
                     /locus_tag="ACHGNS_002791"
     CDS             1528..3480
                     /locus_tag="ACHGNS_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFN6938654.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3552..4049)
                     /gene="dfrG"
                     /locus_tag="ACHGNS_002792"
     CDS             complement(3552..4049)
                     /gene="dfrG"
                     /locus_tag="ACHGNS_002792"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFN6938655.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4376..5068)
                     /locus_tag="ACHGNS_002793"
     CDS             complement(4376..5068)
                     /locus_tag="ACHGNS_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFN6938656.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSWKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1680 a          910 c          859 g         1759 t
ORIGIN      
        1 aatctacgat agtcaacaaa aaaattttca ttatatccag aaaaattttt cttagttaag
       61 gacctatgga ttgcagtaat gcagaattcg tcgaataaac tgccaaagac atacaatcat
      121 attgctttta aaattgacga acaagatata gattcgtttg tctcaaaaat ccaaatgtta
      181 ggtttaactg tcgaaccagg acgttctcgt gtgaaaggcg aggcttcttc aatttacttt
      241 tacgactatg ataatcacct cttcgagtta cataccggta ctctccagga acgtttaaaa
      301 tcctacaatt ccactacctg agactagtaa tgactagtct ctttttatgg cctctgatcc
      361 ccccattgag cgaatactaa tagggcttcg aattgatctg ttcaatcttt aagcaatatc
      421 cctcaaatga gtttggaaag tttagtgatt catcacaaat acttaaatac atgtagttat
      481 atatagattg aggttcttgt gagcgtatgc tcgtgtgggt tcaaatccca tcgtctgcat
      541 ccatctttcc aaaacaagcg gctaggacat ttttgtctca gccgcttgtt ttctagttta
      601 ttctttcatc ttgtcggacc ttttttcttg ggtatagact tttctcatat tctaccacaa
      661 ttttgggttt ccctaacaca tgataggatc tcacaacaaa ttgctttcta ttattataat
      721 aacctgtaac gactaacttc atcccttcgt ctacttcagc taggaagttc aaactatggg
      781 aagcaatcaa acaattgacc ccatttaaac tgaaacggat caacggtctg gcactaactt
      841 gcaaagtacg gatcttatct actgttccat attttttctc cataaccttc acctcaatac
      901 cattatacga acatacattc cctttgtaaa gaagaaactc tgttttatct ttttgacttt
      961 ttgtctatta cctttcttaa aatcatattg gcacttcctg cgaagatgaa ggacatgtac
     1021 taaaaaaaag aggcagcagt atagatttaa gtagttcagt tttggagtac aaaaagtgaa
     1081 aatgtaaacc taaatgagta tcaaattgca actgaatttg atactcgttt tttattgtta
     1141 aatcaacgtt tgttgttaat ttacaccaca aaatgtaagt taaaatgtat ataaaaatgc
     1201 gaacgtgaaa taattattgt aggaatgcaa attggtgtgt taaaatggtt acaatttact
     1261 aagcaaaaag gggtgcattt tcctatgcaa tttcttgagc caaaaaataa gaacgctaag
     1321 tctgtggatt gggaaatatc agagcaagta agggtgatag ttaagcaata cgctgaatat
     1381 gcagagcgta ctgaaagcga agcagtggat gaattcttat tgaacatttt agacgataag
     1441 aagtttattg aatggattgc caacaagcga agtaataagc ggattgttga aaaaatgggt
     1501 atcaaagata gagtgggttg atagacaatg accaagctta aaagattagc cacgaaggaa
     1561 gatgaaattg tagatgttaa aattccgatt tcaaacgaag aattaaaaga tagggctaaa
     1621 caatatgatt tattaactcc aaaacgattt gcaacaaggt ataacaaaat gttgttcttg
     1681 ccaacgtctt ttaaatggaa cggttctgaa tatcccattc aatataatta ctgcatcaat
     1741 ccgttttgtt gtaattttgg gaaagaacag cacaaattca aagatgtaaa agggaaacca
     1801 agtcgatata aaatgactgg tagttccaaa gataaaggtc ataaaggtat gtactgcaat
     1861 gacaatccta ttggcagagg tgtatcgcaa aactgtacag taaccccttt atctaattgg
     1921 tctgttgttg aagaaattaa acgccttata gaaatcaata gcatccaaga tgttgagcca
     1981 gattatcaat ttcataaaga aggctgctcg gaagaagaat caaccccatt caatgagccg
     2041 aagcaatttt acaaaagggg gaaaagtagg ggcaaatctc aacgctatca atgtaaggcg
     2101 tgtaagaagt tcactaatgt tctaccaaaa agagaagaaa ccactactta tcaccaacaa
     2161 aagaacacca ttcttccaat gttcgctaag atggttgttg gaagggtttc agtcagtcgt
     2221 acttgtgata ttttaggaat tggagttgga acctactatc ataagctgga gtggctgtat
     2281 aggcgttgtt tggagttttt agaacgatat gagacacaac cccttcaaac aaagaaattt
     2341 aacgagatgt ggcttaatac ggacaagatg cactactacc taaataacgt ccgtaagaaa
     2401 ggacaaggtt caaagaaata tacggggttt gaagacctta atatgcaaac gtatatagtt
     2461 gtatctgctg aggtcttatc aagatatgtg ttccgttctg atgttgccta tgattggaat
     2521 atttcaatgg atgaattaaa tgaagataca agaaaattca aagaagacca tctcaatact
     2581 tttagtcgta aaaatgatag gttagattgg tcttattatc cacaagaacc atccgctaac
     2641 gatagcgaaa ataggaatgc ttatcttcac gaattaggga aaattactaa taggagtaga
     2701 tttgtcgatg gtttaaatgt tgatgctcct tatactacaa ctgctcacta ttggttaatt
     2761 aaacaaatgg ttaatgccga cgaatggcga atgattagtg atgatgactt ctctattaga
     2821 aacgcctttt atagagtatt cacgaaagaa ttaaggttga gcgatgcaca tcattttatt
     2881 tgccaagtta acaagacgaa aagcagaaaa caatgcttaa aagaattcgg acaagcaaaa
     2941 gcagaactgc tcgattgggg agatatcaga ggattcaaaa caaagtttct acggactttg
     3001 gcatctcatt atctaactga attactaaca agtcatcaat tccacgaaga ggctattagt
     3061 aaagatggag aaagatatcg caaatatgcc gataacccta tcaaacatcc attagccact
     3121 aaggataaag gattttattc agttgattgt agaacggatt tatctgctct ggaaccaaac
     3181 gaaatcgcaa agatgctatt aaatgttaat gaccattcga ccaatagctt tattcaacag
     3241 ataagacgtt atatatcatc tcttgaaaga ccattaacaa cagctcgtgg tgataagaag
     3301 agctatattt acgctaactt caatccgaag tatgctcaat ttgcgattac aattctaaga
     3361 acttactaca acttttgtcg cccttttaaa tcagcggata aaaaggtttt gacacctgct
     3421 caaagattag ggattacaga taaacaattt gattggaaag atataattta tttcaaatga
     3481 caaaaagagc ttggagtaat cccaagctct tgtgctttat ctttactgtt cttcttcaat
     3541 taacgcaccc gttaactcaa taagtttttt ctttcatata catgaaaata gtagttatac
     3601 ggatttttat cattctttat ccctttttgg gcaaatacct cattccattc ctcataattc
     3661 acttctggaa aaaaagtatc tccttcgaat tcatgatgta tttttgtgat gtacattttc
     3721 tcaacataag ggaaaaacaa attataaatc tgttctcctc cgaaaataaa aatttcttct
     3781 tcgtttttac ataactcaaa aacatcttct attgaatgaa caatttcaca accattaaag
     3841 gtaaacccct tatctctcgt cagaataata tttcttctgt caggtaaggc tcttccgatt
     3901 gattcaaggt tcttcctacc taatattatc ggatgtccct ttgtagtatt tttaacatat
     3961 tcccagtcct tgggaatcct ccaaggaatg tcattctctt tgccaatcac tctattctta
     4021 tccatcgcag caatcaaaga aactttcatt tataacacag cctttttaac taatttacca
     4081 tctaatttct tatttctatc aaaaaacctt ttctcattat cctgcccgtt agttgaataa
     4141 gcaatttttt ctataagtta tattatcata ataccacaaa atcacaaatt ttcattcaat
     4201 tatcattttc aattgcattt tataaatcat tttgtatgta atttggtatg taagttaaaa
     4261 tgaaaaaagt agtccatttt gaagtacatt ttgtcatttt gtactccaaa actgaactac
     4321 ttagtataga ttactgttgc ttctttacta atttaggttt acttgttaag aaagattatt
     4381 tacgcaaatg ttcttcaaca atttgctgag atttatcagc tgtatcttcc acttcatctt
     4441 ttgctttttc taacttttga atatattgtt tccagcttga gttttcagca gttgcttcgt
     4501 ctaatttctt ttgtgtatct gttaactgag tttctacatt tgttttttct tgtgtcagtg
     4561 ttgcgatttg ttgattcaaa ccatttacag tattttgtaa ttctgaaagt tttgcttgtg
     4621 cttcttgtag tcttgctgtc caatctgcat tcagatgatt gatttcatca tttttttggt
     4681 tgatctcatc tagtttgaca ttgatttcat tttgtttgct ttgtagttgt gcatccttgc
     4741 tgactaattg actttctaag ttttgttttt ctgctgccga ttgatttttt tggttttcta
     4801 attcatttac ttttgtttgg tattcttgga catctgcctt tgaatcttgc aatgtctgtt
     4861 ctttttgtgc tagtcgagcg gtaagatctg taatcgcttg ctctttatct gtcaatgtat
     4921 gatcaagctg tgtcagattc tcattgattg tatcaatatc tgtctctcca ccccacaaaa
     4981 taatttcatt tgcgtaccgc gtgcctgtgt agccaattag acatagaaat gcaacgacaa
     5041 tcccaccagt aataaatttc tttttcattt gcatagctcc ttaatccaat acttttacat
     5101 gctaaaagta agtgatctca aacacaatgt caagcgtttt taaattacat atgtaatttt
     5161 aatataaata ctatcaaata ctcatttaat cctccttttt tatctatt
//
LOCUS       DAZBTF010000125         5120 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000125 DAZBTF010000000
VERSION     DAZBTF010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.131"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..448)
                     /locus_tag="ACHGNS_002794"
     CDS             complement(<1..448)
                     /locus_tag="ACHGNS_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6938657.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
     gene            complement(755..1852)
                     /locus_tag="ACHGNS_002795"
     CDS             complement(755..1852)
                     /locus_tag="ACHGNS_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFN6938658.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            complement(1960..3987)
                     /locus_tag="ACHGNS_002796"
     CDS             complement(1960..3987)
                     /locus_tag="ACHGNS_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFN6938659.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            complement(4132..4860)
                     /locus_tag="ACHGNS_002797"
     CDS             complement(4132..4860)
                     /locus_tag="ACHGNS_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFN6938660.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
BASE COUNT         1449 a         1038 c          808 g         1825 t
ORIGIN      
        1 tcaactttct cttttcaaaa aaaatttgat atttttttag atttcttttt agtctttgta
       61 atactttata agccacagaa taactcacgt gatgctgttt agcaaaattt tctactgaga
      121 tatatttttc ctgaaagaaa gccaatacca ttttcataga taaagaatcc gttatataac
      181 gagataacag agctttgcta gaacattttc cattaatttc cagacatata tccaattcac
      241 tgacggttac tcgcaaattt ttctctagat cgaacttttg taaatcatgt ttcagcttat
      301 cgatcgtttt tttcaagaga aatgtcgata gcgacaactc ttcttgaaca ttcgatattg
      361 actctgataa atttgagctt gcttctaaat atttaagtaa tgaataactt ctgtgatctt
      421 ctttgcttaa aaaaaactca taattcatta gtcatcagct ctttctacgt atattacttt
      481 ccatgcattc accaactagc tggggataaa actgatttcc tggatccgag tcactgttgt
      541 cagcgattct tgtttgtata atatacatcg tcctgtcttt tttttaacat tttggagagc
      601 gttttatttg gcctttctcg cgatcaaacc tgttattaga attatatcat ttactagtat
      661 gcatttttat taataaaatg gtatatttta tattgttata aaaagagccc aaccatccta
      721 caataataaa aatttactat ttgttatctt aaccttactt ttctttctct tctttttttc
      781 tcaaaacaaa caaaagtatg cataataaaa taaacaataa gaaaagtgaa gctactaaga
      841 tataaatcca attattgtct tttttaatcg taacatccgt cgtgttcaat tccttggcta
      901 cttttccgtc tattgtaaaa tctttttcaa atacccattg attggtatag ttgatcgtag
      961 tatttccttt ttttctcgaa aattttccat tttcattttc atttcctaaa acagtcattg
     1021 taaggtgata ttcacctggg gttagcggtt gaccatttaa agcagttgga aaagaaaaat
     1081 ttgtattcgg cgcaatctgc aatccttctt tttcctcttg atataaaact tcagaatgtc
     1141 ctttttttgt aatttttgtt tcaataacta cttgattgat ataggttttt tgatcatttt
     1201 gaacgtttgc taagatgaca tttcttgcat tgatttgatc tggtttaact tcatgaagag
     1261 taaggttcgg agcaacttcg ttcagtgtct gttgcatcaa caaagcaata atataggtgt
     1321 attcattctc aatcgataag ctttgatcct tagtttctgt ttggtccttt tccgtctgga
     1381 tttcttggaa tgtgatccca ccagccaata caccatcaaa tcgctcattc ggcatcctga
     1441 catgaaaagc aacggtttgg acacttttag gttttagcgt cactatctgt ggatagctca
     1501 cataatctac cagatcaaag atcaggctca tgtctttttc aatctcgttt tttccgtatt
     1561 caaccatcac atttgaattg gtggttgcgc tattcacaga aatatcgatt ttcacttctt
     1621 tttctgtatc atttctcaat tccgctttta cttctacttc ctgatccgga tcaagttgaa
     1681 gatcgaagta agtttttctc ttgtcgattt gattctctga agttatcggc gtcactgaaa
     1741 aattaaatgc actagctgac actgatatat tgttaaaaag tgcccctaaa ataactagga
     1801 gtgttataaa taattgttgt tttggtctca agaaaatcgc tcctttaatc atatgtaaga
     1861 gaagactata acaaaatctt gtctctgtgg gacacaagag cctatggtac ctgtgccctt
     1921 cttgaaactc aataattttg ttatattctt ctcttcctgt taatttcctg ggacatctgc
     1981 aagtatccaa gatagtttcc cctcaaaggt tccgattctt tggttttcta caggaatatt
     2041 tattctgatt ccacgatgag ctgtagaatc taaaagagag gacaaatcgc tttcaccctt
     2101 ttgatcactt tcgaaaatcg taacagcgga tgtccctata accgttgtat tttcagaggt
     2161 aacaaaggaa agagcttctg gaagctcaaa atctccattt gtaagaactt tctcttgttt
     2221 caattgtaat ttccaacttc caaaagcccg ttcatctata attttcaacc cacctgaaaa
     2281 ctgtccgaat tcacttaatg gtctactgga tattttatgt tttccaaaat tgacatattg
     2341 aggagcagtt gagagataaa gtttgccttt tctgtaataa aaatagatat cctctgtatt
     2401 ttcggagaag atgccttctg tattcgctga atccgagtca aaaacatacc cttcaatatc
     2461 aataggagtt tctttatact tctctcctat tttcccagtg attgttttca tttcagcaat
     2521 atcaatctga ctgtctttat ctttataata aacatggata tctatctcct tcagctttgc
     2581 gatctttagg tttatttcat tcgattgctc aatcaaattc ccgttttcaa ggtattcaaa
     2641 atgcatagtg agtacttttt caggaattac ttcctgcaaa gttgtctcca attcgattat
     2701 taacgtatct ccagcaataa aataatctgt cagatttagc gtgactcgat ttccggttat
     2761 caagatatct ttggcatcaa tttgatttcc attgatcaag atatgctcgg gaatagttaa
     2821 ttcttgtggg aggtcgatac tagtctgcat tttcttatat tcaagaaaat ccccctcgac
     2881 cttcaatgta tttctgatag ttatcttatc gtttcctcca gcttcagcga tggccccata
     2941 atcactatcg ttgactttta catcagctgt tgatgtgaca ttcgatggaa gtcgcgtgac
     3001 agcgacagca ttttcttgaa aaaaagtacc tgttgaagaa gtaaaccccc aatttacttc
     3061 tgtagaacca aactgcctaa tgagatcagg tataatccga gaagagcgac cgacaccttc
     3121 caaatcataa gtaagacttc tttgctccgc gtcccattta attattaaca agcgccatgt
     3181 gccattagaa agaaatgtag gagctaccgt cacgccagta tgctctccag aataattatt
     3241 attatttggt ttaggcgtga caaaagcgac atgtccatgc cctctattat cagcagttat
     3301 ctctcgatcc attcgatcag aagaaccact atttttatac gtatcaaact caatcgagag
     3361 cgcattcgca atgtaaggtt ggccagaacg agtagaatat gagccaattc ccatacctgg
     3421 cgatccaata acttgactgg gattactagc cattctagga tcgccagtca atgtaaaggt
     3481 catgccatct cccgctctat ctcgttgatt tcctaagtat aaataagatt taaatgtaaa
     3541 atccgagagt aaactaatct tcttatttga ccaaatagct cctctttgag acctttctgc
     3601 tgggttcaac tgcagtaact tcccattttc caatatggac gtgctagtac cgataggtgt
     3661 ttgaaaaatt ttatcgatag aaataatatt gggatcgtca ggcagctcaa ccgcagaaat
     3721 tggtgcatct tctagtcctt gtttgtccga accctcagct tctacgttgt cagtggtctc
     3781 tccactactc gaaatactta aatcctcact gctttcttgt gtctggttgg attctattaa
     3841 actgtcgaat gtcgaagtga gtttgctttc ttcgtctctt ctatttccat gctccaactg
     3901 attcaattcc aaagagtacc ctatgatcgg aaagatatta gtcactaaga gactgcttaa
     3961 aatgagcacg atcctcctct ttttcaatat atcttttcct cctgatcgtt aaattcctag
     4021 atgaactatt cgtatccaaa caaatttagc ctagcctttg acaatttttg ctcgattacc
     4081 tcgcaagaca atatgactac gtatttattt ttaacagttc ttttcagtaa actagttgtt
     4141 aggtgtatct tctaatgtcc aaactaattt agtggaatat tgttgcgcta acttgacaga
     4201 tttaccgggt accgaaagct gtaccgcatc ttgattttca ttttcatttg caccaaatcg
     4261 atagacccaa gtccccacac ctgttcctga tttagcatta attgctaacg tattgtttcc
     4321 tcccggtgtc aacgttaatt cagaattcac cgtttgcggc gtatatgttg gatcaaccaa
     4381 agaaacagcc tgccctttcg taaatttcag ctgtgctcca actagttcat ccccactggc
     4441 agttttaaac tgttctggtt gagagacact taatttccac ccagataagg tacttctcct
     4501 atctgtcact tggacatatg tcggttttct agacccatcg ttcatcacat cagcagctgc
     4561 ataatacgtt ttatccgcag tagaaatttt ttgagtccca aatttgaatt ttgatccata
     4621 atcaatcgat aatgcccctc ctgttccagg gcttggagga tctgatggat cgacaggttt
     4681 attgggatca ggattctctg gatcaacagg aggagttaca ccctcatctc ctgcttcaaa
     4741 tgtaatcgtt ccttctgtgt tatattcctt tgggtatact tcagttgcag aaacaaaagt
     4801 cgtacttgct accatgctga ataacatcat tgctgctgtt aatacgattt cttttttcat
     4861 cattgacatt cctctttctt tatttactaa acatatcagt cgattggatt aatccatagc
     4921 aaaattctgt tttacgatac ttatgaaata aaagaacata gacctatatt tggtcctatg
     4981 tatcgattag ttataaatga ttatcagccc gatttgattt ctagttttta ttgcaacttg
     5041 atgcaaacac aatctagaaa tacagctgtc atagccttta aaataattcg taagtccccc
     5101 tcctatctat caaaatgtga
//
LOCUS       DAZBTF010000126         5088 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000126 DAZBTF010000000
VERSION     DAZBTF010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.132"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(205..660)
                     /locus_tag="ACHGNS_002798"
     CDS             complement(205..660)
                     /locus_tag="ACHGNS_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LMxysn_1693 family intestinal colonization
                     protein"
                     /protein_id="HFN6938661.1"
                     /translation="MKKQLFKTFLLSTSILTFGSAITPSGAVFANENNISDIATYSDD
                     EYYYVLDEPVIQTRTWSKPQSWSIAKGSRTYLGIETHSRGTKLSVSANVFGMKIEIAR
                     NTSASGKFKKYRQNARITVTYRVYRKVDNKYIRTQTSTANTQYIDYIPA"
     gene            complement(1269..1685)
                     /locus_tag="ACHGNS_002799"
     CDS             complement(1269..1685)
                     /locus_tag="ACHGNS_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938662.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTITTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            complement(1687..1968)
                     /locus_tag="ACHGNS_002800"
     CDS             complement(1687..1968)
                     /locus_tag="ACHGNS_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938663.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            complement(2415..3383)
                     /locus_tag="ACHGNS_002801"
     CDS             complement(2415..3383)
                     /locus_tag="ACHGNS_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFN6938664.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            complement(4550..4705)
                     /locus_tag="ACHGNS_002802"
     CDS             complement(4550..4705)
                     /locus_tag="ACHGNS_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938665.1"
                     /translation="MKLTKLTISLVIIAGIILYFVLPANQFFLYFGIPTILIVNGVRI
                     FEQRKKM"
     gene            complement(4724..>5088)
                     /locus_tag="ACHGNS_002803"
     CDS             complement(4724..>5088)
                     /locus_tag="ACHGNS_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFN6938666.1"
                     /translation="FSVLFLINILNTSFSEGINPNEVNGSLLLFFLNAATYGAFLEEG
                     IFRFCMIDPQANKKQQYISILISSFLFSIVHGGGLSIFFIGIILSFVYIQTKNIWYSI
                     VAHGFYNTIGILIYLISI"
BASE COUNT         1595 a          839 c          839 g         1815 t
ORIGIN      
        1 aaaaaaaact atatttaaag agttttttta gatttacttc tttgaattca cgaatgaagg
       61 tagaatgaaa tagtataatt attagcacta aaaaaaatat atcactatgt tcaaataaaa
      121 aatatatatt tgacggtact cctaatgatg ggatagcaaa ggtcaaaacc aagtaagcaa
      181 tactaattat ccattttatt gtcattaggc aggaatataa tcaatatatt gtgtatttgc
      241 tgtagaagtt tgagtacgaa tatatttatt atctacctta cgataaactc tgtaggttac
      301 agttattctt gcattttgac gatatttttt aaattttcca cttgcagaag tgtttctggc
      361 tatttcgatt ttcatcccaa atacatttgc actgacagat aactttgtac ctcgactatg
      421 agtttctata cctaaataag tacggcttcc tttagcaatt gaccagcttt gaggtttaga
      481 ccaagtcctt gtttgaataa ctggttcgtc taacacatag tagtactcat catcagaata
      541 agtggctata tcagaaatgt tgttctcatt agcgaatacc gctccgctag gagtaatagc
      601 agaaccaaaa gttaaaatag aagtagataa taaaaaggtt ttaaataact gttttttcat
      661 caaaatcacc tcgaatataa gatacttaag aaaacgcttt ctttatataa ttagaataat
      721 caggaaattt aatgttgtca agagggttta ttttataaaa gctgttatat taaaggttag
      781 tagacataat gtacactatg ccaagtagcc cctctaccaa tggatagaga cgctcacttt
      841 gtgatttcta attggtctag cttaaacact tcagccaatg cttgatttta tgcacttttc
      901 ttttttcatt tctttgtgca ttttgaccgg tcatttttta gagatttgtt atttttgtga
      961 gcatttgata tgctcttttt atacctcaat ctcttctttt ctaatgttaa tgaataggat
     1021 aatctcattt ttagctgctc gtgagagcag tctttttttg tttcagggta cgtcctattc
     1081 tttggatttc cgcgctatta aataaccgat acttggggat tggggtagcg ccccaaaata
     1141 caaaaagaag aagttgcata agcggatttt tctttttttt cgcaagttgg taccaacttg
     1201 caaatcttgc tggaagactg atccctaaaa agaaatcgag gaattttttt agggatcggt
     1261 ttcttttttt atctttttag aaagtttgtt gggtcaaaat ttccagaagt aaaggtttga
     1321 ttggtttctt tttgacgatt tttgggttga tttcgtcggg aaggattgag ttgattttca
     1381 attttttctt tcacttcttg atacacttta gaatttgtac cgataagaac accagtatct
     1441 tttagatata aatcagcaaa ttctgccatg atttctgtca tgatagaaat ttcttttccc
     1501 atggcattga gttttgcttg gttactggtt gtgattgtat tggtatcagg ttcataactc
     1561 atacataaaa aacgaatggc ttctgaataa ttgttgacgt ctggattttt ttcgatgatg
     1621 gaatctaaaa cgtcaacgtg actgggataa agggttacgt gtttttgaac agaagttttt
     1681 ctcatgttat attcctccga taagtgtttc aatgagttgg ttgtttgtgt gaacatagtc
     1741 attcagtatg ttcgtatttt ctgtttgaat ggcaataaat ttttcgagta ataaatcgaa
     1801 ttgatctttt ttgtaattca taagttgacg gtcaaaatca ctttcgatta gaacttttag
     1861 atatttattt cgactccatt ttttgcccgt tttggttgtg agttcctgac aacgtttatc
     1921 tattcttgca ataattttgg gattgatatt tcgaactaaa atgtccatgg aaattttcct
     1981 ttcgtatcag cgtataattt atgtatatca tacaaatata gtatttgtat agctattttt
     2041 cgccaaaaca ctatacatag cctagccaca atggcaaaaa agtgatagca aatttttttc
     2101 tgagaaacgt gataggcaca gcctatctag caccttttgt aagaaagtga taccacgttt
     2161 ccttatgctc tttcaaaatt cagaaaaatc agctgtaaaa cgagtgcagt ccgagtaaga
     2221 ttcaacgaaa aaacggggat tggggcgcca ccaaaatatc gcaacatggg tccatgttgc
     2281 tctgctcgca aaagcaaaaa gaagaataaa accagcaatt taaattaata aaaattgctg
     2341 gttttattgc cagaattgcc accatttttt ctccgtcttt gattgttttt tctgtcataa
     2401 aatctaaaaa cccctcaatc tttgaataag ttttgaagat tgtctaataa gtcttttttt
     2461 agttctgaag gaatatcgga atttatctct gatttttgtg cattattttc tttattttgg
     2521 gtattttgtt ctctttctaa cttttctaac tgttgtaaaa gttccaattt tcgcttttgt
     2581 tctttgattt tttcttgtaa tgaagtaggt gctttttcag attgaacttg ttggttagct
     2641 agttctaatg ctttgttttg ttcttctttt gttggggcat gtttgttaaa gtcgtctgat
     2701 ttttttatct caggtttaaa tgtaaattga tagccaatga caggctttcc ccgtcctttt
     2761 ccgtatttct ttttgattgt taaaccttta aagattgcag ttagttcttg tcgtattgga
     2821 ttgaacacac gttgatcaat cgtaccttgg ttgtaacttt taggaatatc aagtaattca
     2881 tgaaagtctt cttttgaaaa ataagcgaaa cctttggttc tgtattgttt tagtaatcga
     2941 aacattgttt ttgaataact gctttctaat tcattgaatt gttggagaga atagcgcacc
     3001 cattcttcaa gattattaag taagggtaag gctttttcat gaacttgtat ttcagcaaaa
     3061 ggaatatcag attttccgtc gattttaaat tgattaaata aaacaaaacg ttcacgttgc
     3121 aaaccgtcag cacttcttct accaaaacgt aaatccatta atttgtcata cgttgtttct
     3181 aaatcatcta tgaatcgaac atttgcagtt gctttataat tgcttaaatc ttttaattgc
     3241 tcgaatgtaa attgtacttt tttatcacct acatcacgca ttcttgatac gatagaaaag
     3301 aacaaattca tttcgactgg tgtaaatttt cgcaacggaa ttgtatttaa ttcatgatga
     3361 tactttacga tttcattaga catatttaaa atagctcctt tcttactcgc acaagagata
     3421 cacgttatga aatacaaaat gcctatttat ctacatctta tattaacatc gtcgatgtac
     3481 taattcaatg tacaaacaga caaaacgatg tagataaaca gacaaaacga tgtagataaa
     3541 cagacaaaac gatgtagata aacagacaaa acgatgtaga taaaagcgtc attcccttgg
     3601 gagagtaagg atatagccga cggtaaagag gtttaaaaag aggtttaaaa agagattata
     3661 aaagaggggg acaaaatttc ccgcctaaaa aaccacttct tttttctcca aacagattcg
     3721 cgcatttttc tcaactggga atcttactcc ctacagtcgc ttaaaaaggt gtgatcgctc
     3781 tgctctcaca ttatactgcg cagtcgcttc cggctccttt gctaaaaata ttcataagga
     3841 actctttgag aagctatgag ccttcgtttg agacgtttta agacagtctt agtataaatc
     3901 ccttatcttg ttcttaaagc gccttacagg cggtcagggc aagccctaac aaccctaaag
     3961 ttgtctgaat gccctctaaa attcgattta agcactctta aacctattct tatatattta
     4021 tatacttaga cgtttcaaac gtcttaaaac gcaaattaga gcttgataaa gatatttgct
     4081 cgtgtgccga cttttttaaa agtctaggca cacgccatga caaagctgaa ataaactgct
     4141 tcagctttgt catggcggat tcgctcactt gaaaaagtgt tcatttcgct cgattattag
     4201 catatatttt ttaacttttt ttgctagcca aattttgcat tagttcgctc ataaatccca
     4261 ccctttcagt acgtcctacg gacgtatcgt tcgtaatgtg aaagtacctt taaaggtact
     4321 ttctacaaaa ctcactaaag ggtttggcaa gctctatatt tgctagccaa atacctacgt
     4381 atttggtgca aataaccgct tgtcaaaggg gcgcaagctc ccccttgaat cccccctcaa
     4441 aacccacgct ccacaataca aaaaaggcta gcaaatgcta gccacttttt gtaaagtttc
     4501 gcttgtttca ccttcggtga aattgttgct ttgcaacgat caggtagagc tacatttttt
     4561 ttcgttgttc aaatattctt acgccattta ctattaaaat cgtaggtatt ccaaagtaaa
     4621 gaaaaaattg attagcaggt aatacaaaat ataaaataat accagcaata attaccaaac
     4681 ttatagtaag ttttgttaat ttcatgaagt atgccacctt atcttatata gatatcaaat
     4741 agattaatat gccgattgta ttataaaatc catgagcaac tatggaatac catatatttt
     4801 ttgtttgtat ataaacaaaa cttaaaataa taccaataaa gaaaatactt aggcctcccc
     4861 catgaacaat agaaaacaga aaagaagaaa ttagtattga aatatattgt tgttttttgt
     4921 ttgcttgagg atcaatcata caaaatcgaa aaattccttc ttctaaaaaa gctccataag
     4981 tagcagcatt taaaaagaat aaaaggagag aaccatttac ttcattagga tttatacctt
     5041 ctgaaaaaga tgtatttaga atattgatta ggaataaaac agagaatg
//
LOCUS       DAZBTF010000127         4966 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000127 DAZBTF010000000
VERSION     DAZBTF010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.133"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(15..88)
                     /locus_tag="ACHGNS_002804"
     tRNA            complement(15..88)
                     /locus_tag="ACHGNS_002804"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(52..54),aa:Ile,seq:cat)
     gene            complement(277..1296)
                     /locus_tag="ACHGNS_002805"
     CDS             complement(277..1296)
                     /locus_tag="ACHGNS_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFN6938667.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RADVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1293..1517)
                     /locus_tag="ACHGNS_002806"
     CDS             complement(1293..1517)
                     /locus_tag="ACHGNS_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFN6938668.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            complement(1514..1807)
                     /locus_tag="ACHGNS_002807"
     CDS             complement(1514..1807)
                     /locus_tag="ACHGNS_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938669.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            complement(1844..1981)
                     /locus_tag="ACHGNS_002808"
     CDS             complement(1844..1981)
                     /locus_tag="ACHGNS_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFN6938670.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            complement(1983..2414)
                     /locus_tag="ACHGNS_002809"
     CDS             complement(1983..2414)
                     /locus_tag="ACHGNS_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938671.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            complement(2428..2922)
                     /locus_tag="ACHGNS_002810"
     CDS             complement(2428..2922)
                     /locus_tag="ACHGNS_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938672.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            complement(2922..3371)
                     /locus_tag="ACHGNS_002811"
     CDS             complement(2922..3371)
                     /locus_tag="ACHGNS_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938673.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            complement(3375..3995)
                     /locus_tag="ACHGNS_002812"
     CDS             complement(3375..3995)
                     /locus_tag="ACHGNS_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938674.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            complement(3995..4903)
                     /locus_tag="ACHGNS_002813"
     CDS             complement(3995..4903)
                     /locus_tag="ACHGNS_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFN6938675.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
BASE COUNT         1352 a         1076 c          791 g         1747 t
ORIGIN      
        1 aatggctacg ttaatgtacc ctgtaggact cgaacctacg accggacggt tatgagccgt
       61 ctgctctgac caactgagct aagggtactg gttgttgcca cataaagcca taaacaatca
      121 accagtagaa tgtgtggcaa caaacctgtt atcgcatagc ttggagtgtg actattatgg
      181 gtgatagtga agatatgcga taacatcact attttatcga atgattttta tagttgtcaa
      241 tatagttatg taatgctcct caacgaggag ctatttttat cgtttaggaa tatttaaata
      301 ccaacgtttg tcatggaaat cttgcgcacc gcctttagtg tttccctctg gatcattcgt
      361 tgcccgcatc atgacataga ctttcttatt agggaagtta cgcatgttaa aagatacatg
      421 atagccaaca tttccagaag tattataagc ttgatttaca tctgctctat aaattccatc
      481 agctcttact cgagctaatt ctttcccagt attgtaatcc ataatgaaga tatactcgta
      541 tttatagtta gcaatgtgcc atccagctac atgcaaattt gcattttcga tttctccaaa
      601 ctgatcaatg tgggcgtaat tcgttccatc tgtcagtgta ggattagctg caccagctcg
      661 tgttggatca atgacaggct tgttttcaga agttgttgga ttttcatcgg taaatccatg
      721 agctaaatca tatgctaatt tttctttact tacgcccatt tcagaaagat aaccgtaagg
      781 atctgtatga tcgccccaga tgttttgtgt tacccataaa tgcgatttga ttcccggttg
      841 gttataagga gtgtccaacg ttaatggaat accatatttc attgctgaat ctctagccaa
      901 ttcaacgtat gccttgtagt ttttctcaaa cgttgcttta tcatgtgtgt gttgtaactc
      961 aatctgcaca ggactgttgg cattagcata cgaaccagca ccgtactgta cataaccagg
     1021 ttgaccgact tgataaacaa ttccgccgtc tcccacaata taagcagtat aagcgctagt
     1081 ccatgaacgt tgcatatact gcgcttcatt gcgtcctgtt gctgtttcat tagccgtttc
     1141 atgcagtaaa atatactgat tatttgctac ttgtgagcta ccttcatttg cgcccaaatt
     1201 aaattcattg ttgatagtat aggcaaatcc gttaattggc aataaaaaaa gagccgttaa
     1261 caggctcatc gcagtaatag taattttctt tttcatttgt ttcctccttc ttcgctttca
     1321 gccgagaaca ttttgtaggt tcgatttgat acacccaaca cactccctaa aaacgcgcca
     1381 aaaccagtaa tgatgacaac acagatatct gtgtactgcc aattgagcgc tttaccaact
     1441 aaccccacga aagtagctag tgcgggaata attaccagtg cgaaccattt tagtacttcg
     1501 aacgttttat tattcatttt cttctctccc taaacaaagt tttgatttgt tgcgtgtgtt
     1561 ccaccaattt ttctgtatgt gtatctaatc tttcatcgtg tttctttagt tcttcatgaa
     1621 tcatcaatcg atctgatttg ctcgattcta aatctttagt cagcaaatct aaattgtggc
     1681 ttacttttga aagagtctca gtaattttcg agaaagatgc agcaatcggt ctaattacta
     1741 ataaaatcaa agaaacgata gcggttattg atcctgctat cattccccat tcccctaaat
     1801 taatcatgtg acaactcctt taccttaaat aaaaacgcat caattaagat gcgctcttat
     1861 ctttattaat gattttatct gcttcttcgt ctgtaatgca taatggaacg aaaaccatta
     1921 cttgttcgtt agtgaaacag ccccagtcat acattagttt gatatcttca tatgaataca
     1981 tgttacttcc caccttgtaa ttgttttttg atttcttcga tttccttggt attttgtaca
     2041 gaagcaagca tcgtttttga attcaactgc gctaaacttt ctgacttttc tttcaataag
     2101 acattttctt gcttgattgc tacattgttt agcattgctt tagaatttag ctgtttcaat
     2161 tcatcatttt ctgccttcag cgtttcgtat aatgccttaa tattagccaa ttcatctacg
     2221 ctatcaacag cattcccttt tttctcttct tcagtagcta actcaaccca ctgtttttta
     2281 tcaaaatcaa atcttggtct ccagttagga actggtggtt taatctctgt acagttttca
     2341 gggatatttt cttgattgtt caaaatgatt tgctcgaatc cgtaaggctt gattgatttg
     2401 taaactactt tcattagatt tcctccctta aattgaataa gtgataacaa atgaataagc
     2461 tgatccataa cttgaatttc ttctccattt aatggctccg tctgcgccaa tagatagttg
     2521 agcactgttc aaagtagaac ggtctattga cccaaccagt tgttcaaaac taattggtgt
     2581 ccgatagcct tctggaacag ttaaaataac cgaatcattc ccgccactac tttttccgtt
     2641 taaagccaca aaatatatag aaactgtttt tccttcacga taaagctttg ctgtccctgt
     2701 attaccgtta gtgacaacca gatttaccac ttcatatctg ttatcatcag tcgtcaaaac
     2761 acgtttatta ttgattgtta cagaacctga aaaattctta tgtcctgcaa tggcttggtc
     2821 tccatctgtc tgcaccaatg cttctttacc atcaatagct tctacatgcg tttttaagta
     2881 tttagcaact ccgtcttctt ttaattgaac aatatcagcc attaaaccgt ccccactttc
     2941 tcaaatgtaa tattcgctaa tccatcaagt ttcactttat cggttgctga cattaaacct
     3001 gctgtcgtag ttgtagcatt acctggattt ttctgtgctc cagctgcaat tccatccaac
     3061 ttagttttat ctgtggacga catcaaccca tttgctgtag ttgtagctac agctgtagtt
     3121 gtggcattta ttccaggatc acctttatct ccctttggta aaacaaaatt aaatctagct
     3181 gcagatgatg ttcctacatt cgtaacagaa gcggttgaac cactagaaac ggttcctatg
     3241 gtaattgttg ctgcttggcc aggatcgcct ttatctccct tcaccgttgt tggtttgcct
     3301 tctatagcat tccaatgagt ttgtggataa acctgtacat tgctttgttt tatttttacg
     3361 atatctgtca ttctctatac ctccccaatt ttttcaaaag taaaatttgg aattctttcg
     3421 tttgtgtaat tttctgcttg atttacagct tcttggaatt tttgatctac atatgactga
     3481 ttgacaccac cagtcccact accacctgta gaactaattg ttccatcttc atcaatagtg
     3541 atgttttctc cagcagtata atttttcagt gaatcgagtt tttcttttaa ctccagcgaa
     3601 aaattgacat cagattgtct gactgcagag actttccctg tctcatcaat cgtcaataat
     3661 tcaccaactt ttatgccacc caaacggtcg atagtggcaa ctggcaacac gtagtcacct
     3721 tctccactgt ttaaaatttt ttgatacatt tctgccgtga taatgccgtc atcttcttca
     3781 cttgcatagg gtaattctgt gagaacattc tctaatccca aatcttcttt agtaatgacg
     3841 acggctccaa tttttccatt aacagataaa actgttgctt ctcctgctat tatttcttct
     3901 aacccaagaa ctgcggaagc atgtgtgatt ggaaagaatt ggcgttgtac acccgttttt
     3961 tcatccgttt ccatcattcg tttagtttta gccattagat caccccaact ttctcaagag
     4021 tgaacacatt ttgctttgga tcatcaacgg ttgcgatgat caaggctcct tcttcaatcg
     4081 ggaatgctac agttccaact ttttcgactt catgattatc tgaaaagaga tcatcctgca
     4141 aaatatcagt cacttcaatc tccccgtatt caatcgtgaa aagcgttgct cgcaatttct
     4201 gatataaata gtccatatct gccagcaaac gctctgaaat agacgcatga cgaactcctt
     4261 gaatatctac tcgcgcatcc gttagttcgg ctaacatgac tccacctgga tcaatcgtct
     4321 ttaaaatatc cttgattgat tcaaaccatg ccaaatagtc tgattcttgt ccttctctcc
     4381 aatcttggaa actgtgttcc tgttcttgtc tccaacgttc aaattcttct ttcctttcat
     4441 tcatccagtc cgtaaaatcg cctttatttt cattaataaa gtccgtcata tccgcaatta
     4501 aatcttcgat tgattgccaa taagaaccca tttctccttc tgtttttgaa acagcattaa
     4561 tgacaaaata agagaagtct tgagttgttc caatcaagtc ttctccttta aatataatga
     4621 aattggctgt ttgtcgatgt aaacactgca tggaatattt atcaaaaata tagttaattt
     4681 tccctttttt agcatccaca atttttgttt ctaattggac tggatatttc ccacctacaa
     4741 ctgattcaaa atatacctta cattctgata aatcgtaggg aagaccattt tcaactatag
     4801 tcgcttccat aacctctgta ttcttattgc cttgtcgaac ttgaatcatc cccacgtaat
     4861 tatagggttc tgttgtactt aatatgacat tccactttgc cattaaatca cctccctatt
     4921 ttggtggtat gacaatggaa gaaattgcac ttgcactata atactg
//
LOCUS       DAZBTF010000128         4961 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000128 DAZBTF010000000
VERSION     DAZBTF010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.134"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(139..1338)
                     /locus_tag="ACHGNS_002814"
     CDS             complement(139..1338)
                     /locus_tag="ACHGNS_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFN6938676.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(1468..2844)
                     /locus_tag="ACHGNS_002815"
     CDS             complement(1468..2844)
                     /locus_tag="ACHGNS_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6938677.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2844..3500)
                     /locus_tag="ACHGNS_002816"
     CDS             complement(2844..3500)
                     /locus_tag="ACHGNS_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6938678.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(3510..4787)
                     /locus_tag="ACHGNS_002817"
     CDS             complement(3510..4787)
                     /locus_tag="ACHGNS_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6938679.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            complement(4806..>4961)
                     /locus_tag="ACHGNS_002818"
     CDS             complement(4806..>4961)
                     /locus_tag="ACHGNS_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFN6938680.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
BASE COUNT         1556 a          903 c          608 g         1894 t
ORIGIN      
        1 tcggagtaag tggcttcgac gattggataa ataaattcaa aatcaagcgc ggcatcaatc
       61 tttcgaacga gatgatcttg aggaactaga tcatcaatcg taagcatcgc aaattgattt
      121 ctaccattcg tattgttttt agacatcata gctatcactc cttgcattcg ttttcataaa
      181 tctattataa aagaaaagac tgtagacatc atccataaaa tggactttgt ctacagtctg
      241 cgcattctca ttagagaaca ctcccttttt tgtgactttt ctgaagctag agtgaatact
      301 ttcaactgca ttagtagtgt acatgatttt tctaatggcg ctgccataat caaagagttg
      361 ttcaacatga gaaaagtttc gtttgcatac atctactgca ccagaataat gagaccaccg
      421 attttgaaaa ctgtcgaagg cagcgtgtgc agcgtttaaa gaagaagcac tgtagaattt
      481 tttcatgtct cgacagactt ccttataatc cttacttggg aggtatcgta acgcatttcg
      541 aacaagatga acaatacagc gctgcacaat gacagaagga aaaattgctt ttgcgccttc
      601 tttgagaccg gaaacaccgt ccatcgaaat gaaaaaaaca tcttctacac cacgtacttt
      661 cagttcatca aatacttgca tccagcgatt tttagattct gtttgattga gccataggcc
      721 taaaatctcc ttgtttcctt taagatcata gccaagaatg gtgtacacag catattcttt
      781 agcttcatag ttttcgcgta aagtgacgta catacaatca acgaatagaa atgcataaca
      841 cttggctaat ggccgagctt gccattcttc caattcagga aggacggcat ccgtgatatc
      901 agaaatcatt tcgtgggaaa tatcaaagcc gtaaatatct tcaacggttg cggcgatatc
      961 gcattgactc attcctcgtg catacatgga aagaacctta ccttcgatgt cggagacatc
     1021 tcgttttcta ttaggaatca actcaggttc aaaagaagcc tcccggtctc tgggaacatc
     1081 aatgtttact ttaccaaagc tcgttttaag cgtcttattg ccataaccgt ttcgtcgatt
     1141 gtcatgtttc tttggctctt tagaatgggc gtcataccct aaatggttat tcaattctcc
     1201 ttgaagcatt ttttcaaata agggaccaaa cacatctttt aaaacatctt gcatatcctc
     1261 tacagattcg ggttgataag tattcaaaat ggattcagct aacttctcag catcaggatt
     1321 tcttttcttt ctagccatcg tgtaatcacc tcttgatctt attttagaaa aataaaaacc
     1381 gtgaagcaac cacccgccta ggattgatgg ttacttacac ggtttatatt acactctcat
     1441 ttttggtgct aaaaagcacc gaaaatttta attattgtca attaataatt cttttggatt
     1501 ccttcttaat atattgaatg aagaaataat caatgataca agaagtacta aactcataaa
     1561 tataactaca taaatcatga atttaggtaa tacattgata tctaaacttg ataatgtctt
     1621 gttaaatcct tcagcttctg ccccaccacc taattggcta gatgcagatt gtttagctat
     1681 ttgtttagct atatcaccag taaccttatt caatatatta ttcccaagct tgtcagctgt
     1741 atattgagct aagaaatagg aaccaacgaa tgcggggatt gatataaata ccatctcaat
     1801 taggaattga ccaaaaattt ctaattttga tatacctaat gatagtaata ctgctatttc
     1861 tttctttcta gcattcatcc ataagaataa taataatgaa actacaactc ctgcaaatat
     1921 taatgagcca aaaaataatt tatttgaaat tgagtaaata cctgatatag attgttgtaa
     1981 tgcagggtaa tttgatgaac ttttaatcaa attatattct ctccaattta tatctaattt
     2041 cccaagatct tttattacac tatcaagatt cttatcacct tttacaaaga atgttgcatc
     2101 ttggtataca gctgtatctt cggtattacc ataaacttta gcagctgtat gaatatcagt
     2161 gattaatgta ttttcgtata gttcttgtgc tgcacttact ccaccgctat tatgcccatc
     2221 gaatagacct ttaatttcta cttctactgt ttcatctgca cctttctcat tatcagcatc
     2281 aaataaatta gattttattt ttatcttatc tccaacttta aggttatttt tttcagctaa
     2341 atctttatgc attaagattt tatttttatc ttcattttct aaatgctttc cttctactaa
     2401 tttatatgct tctgatacaa attttgtttc ttttgttgag tcgttaaccc cagttaacat
     2461 aactgttctt ttaaaattct ttgctctttc aggtgattga ttcgcaagag tctcttgagt
     2521 ttcaataata tcataatcaa ctaaatctgc aacactgttt attcttttta cataggagtc
     2581 tatactatct gtttgagata tctttttaat atcttcacct tttacatttc ccccacctct
     2641 aggtgttcct ggatttactt gtctatttat ctccatagaa aaactatttg ttatattagc
     2701 aaatgtttct tttgaagctc tgtccgtagc atctttaata gataaactaa tgagacttaa
     2761 agttgacata gataaaataa ctaatatgat aattaatgat tttaaacttt ttctagttac
     2821 ataagcaaat gcatttttta tcattattca acctccaaat tcattttatt tacttttttc
     2881 aactttttac cacttagttc taaaatgata tccgcagaat ctgctacttc cttactatgt
     2941 gttacaacta ttacacattt atttctatct ttagctaatg tctttaatat attaattatt
     3001 tctccagcag taacactgtc tagattacca gtaggctcat cagctagtat tattggagca
     3061 tctgatacca atgctctagc aatagctact ctttgttgct gtccaccaga taatttcata
     3121 acatttcttt ttatttgttt tttatctaaa cctagttcaa acaagatact ttcatctgct
     3181 gatttattta ctaatctaat attttcaatt ggtgataaat aatctattaa attatagttt
     3241 tgaaatacta aagatatatt attttttcta tgattactat atcctttatt ttgtatatct
     3301 tcattcttaa acaatatttt tcctgtttga ggtttatcaa gtccagcaag taaggaaaga
     3361 agtgtagatt ttcctgttcc tgactttcct actatcgcat aaaacttccc aagttcaaat
     3421 ttttgattta ctcctgacaa aactttttct ttagaatttg cataactata tgctacattc
     3481 tttatttcta atatatccat tattttctcc taacttattt ttgataagat ttctttaggc
     3541 ttcttgatta acattaatga agaagcaaat acaactgata aaacaataat acttattaat
     3601 attaaataac tttgtccaag tgttgttata tttgacataa aactactatt atttattaaa
     3661 cttccaccgg aaatcattga atcctctgag ttaatgaatc catctacaat tacttttagt
     3721 aatacatttc ctaaaaataa ggaagatatt atacttggta tcgatatgaa tattaactcg
     3781 aatataaatt gcattataat ttgtatctta gatgttccaa tagataagaa tatacctatt
     3841 tcataaattc tttcccttaa ccatagaatc aagattaatg aaagaacaac catcccacct
     3901 aacataatag aataagtcat tacttttatt atatatttta ttccactcac tgactctaaa
     3961 gactcttcaa acgcattaga atctttttca acaaaatact ttgactcatc aattttaagc
     4021 tctttcaatt tgtttaaggc taagtctgta gattctgcac taccagaata cattaaaatt
     4081 ttatttgcaa tttcattatt ttctgaatta tttaatatct cttggcttgt tgaataatct
     4141 acaaacacca tattttcact aaaatcagaa gataatcctg tatatgtttc ctgtttttta
     4201 ccagaaaaga tccctataat tttaaattta tgacttttta tttttccact tttttcaata
     4261 tctagtaatt caagatcaac ttcatcacct aattttagat tgttttgttt agcaaattct
     4321 tcatgaacaa taattgaatt cttatcattt tcttctatat ttttaccttc tttaattgta
     4381 aatactccac tactaaataa aatatttctt ttagtattat ttgtagcttc gagtgaaaca
     4441 acattcttaa attcgtcaga taaatcttct ctatttattc tttgttctcc gctaactact
     4501 ttagcatctt ttagttttgc taatccatca tattgaatta ttttttcttc aatttctttt
     4561 aatttttcaa tatctttaaa ttgattaaca ttaaaatatt taccatcttt ttttgttatt
     4621 gatattgaag aattagaact ttcatataaa gccttttcta tttcattgct tgatttcatt
     4681 attgttaaac aagaatacaa gcaagaaaga actatagtta atatgataaa aattattaaa
     4741 gttctatttt tctttcttgt aatatatgct atagcatttt ttatcacttt cctaactact
     4801 cctctctatt ttcttactcc ataactaatt gcatccactg gacaagcatt tttacatcgt
     4861 ccacatctga tacactccag atgattgcaa ttctcaactg aatctttatt catttggcaa
     4921 acttttttac attttccaca attaatacac ttatccttat c
//
LOCUS       DAZBTF010000129         4948 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000129 DAZBTF010000000
VERSION     DAZBTF010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.135"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACHGNS_002819"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACHGNS_002819"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFN6938681.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="ACHGNS_002820"
     CDS             complement(1297..2133)
                     /locus_tag="ACHGNS_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6938682.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="ACHGNS_002821"
     CDS             complement(2317..3534)
                     /locus_tag="ACHGNS_002821"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFN6938683.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3844)
                     /locus_tag="ACHGNS_002822"
     CDS             complement(3683..3844)
                     /locus_tag="ACHGNS_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938684.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3917..4000
                     /locus_tag="ACHGNS_002823"
     tRNA            3917..4000
                     /locus_tag="ACHGNS_002823"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..4948)
                     /locus_tag="ACHGNS_002824"
                     /pseudo
     CDS             complement(4240..4948)
                     /locus_tag="ACHGNS_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1514 a         1024 c          794 g         1616 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa
     4801 taaaaataag atattcatgt ctaaaaaaac ggctttccaa atgatgaaat acctaaatct
     4861 aacttattaa gaaagttgaa atttttatat tcaggaaacc atttattaat ttcatgtgca
     4921 gcacgtaatg cattccctgg agttgtca
//
LOCUS       DAZBTF010000130         4902 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000130 DAZBTF010000000
VERSION     DAZBTF010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.136"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            30..329
                     /locus_tag="ACHGNS_002825"
     CDS             30..329
                     /locus_tag="ACHGNS_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6938685.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
     gene            594..1469
                     /locus_tag="ACHGNS_002826"
     CDS             594..1469
                     /locus_tag="ACHGNS_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938686.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIKRVFRNIYYEKRAVTLSNVMYIVLNVHKLIEIT
                     FPTLDCEKKEYMPKIRQRGIEKKRYVSLKKGRGMYKFVYTYTKTTSQNISKTTLVMEE
                     EDINIKRIYSKISVLYDANNEDGRKLFQVYHYLRNANVDTEDIRKIIEYLSENTQYLI
                     AECIIKQHEACFFQRKTEEGLYDYVTYFLNGLQKRYKSQFLENVEPIDQFKKAFEIKE
                     LPEITMHNWLKGEES"
     gene            1466..1684
                     /locus_tag="ACHGNS_002827"
     CDS             1466..1684
                     /locus_tag="ACHGNS_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938687.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            1671..4298
                     /locus_tag="ACHGNS_002828"
     CDS             1671..4298
                     /locus_tag="ACHGNS_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFN6938688.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENVQNSTSSQGFKSMDVRENVQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENV
                     QKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNE
                     LFRDTLNDPNPLFDNLLKK"
     gene            4313..4531
                     /locus_tag="ACHGNS_002829"
     CDS             4313..4531
                     /locus_tag="ACHGNS_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938689.1"
                     /translation="MKLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKHEIKNNLKYCYLEKSEEIIHELNGF"
BASE COUNT         1920 a          611 c          981 g         1390 t
ORIGIN      
        1 gaataattaa ttaaaaagga gaggtttttg tggaagcaat aaaattaatg atttcagaag
       61 aacaggagga aatgtttcta aagcagtttt atcaaattgc tacaaaagct attgaaaagg
      121 ctcaagaaaa tgcggggatg acaagggaat atctcaatca aaaagaaatg gcggaatact
      181 taaatgtgag tgaaaacttt ttaaaaaatg ctgtgagaaa tgagggattg cccgtagttg
      241 aattaggggc aagaaaattt tattcgaaaa aggcagttaa tgagtttatg tttgctcgaa
      301 tgaaaagagg ggaaaatatt ggaagatgat gaagtagaaa ttataaaaaa atgctttaaa
      361 aaaagaaaaa aagtgttccc aaaagtttgc agactggggg aacactcaat aataaagtgc
      421 tgagaaaact ttatttatag ctattatatc aaaaaagaag gtgaaaaggt gttttcttct
      481 ggaaatgttg tagttgacaa acttagagaa ataaattttg acggaaatgt aatccacaca
      541 catggtacga tcaattaaga aaaaaaacga aaaaaggtgt tgagaagccc tatttgaatg
      601 caatagttat cttagcagaa attacctatt ggtatagacc taaaaaaatt tttaacgatg
      661 atggtcagct acttggttat caaaaaaagt ttcttgaaga tattttgcaa aaatcatata
      721 agcaattaag caaaaaaaca ggattatcag aacgagctat aaaagatgca attgtgtact
      781 tagagaaaaa ggggattatc aaaagggttt ttagaaatat ttattatgag aaaagagcag
      841 ttacattatc aaatgttatg tacattgttt taaatgtaca taaattgata gaaattactt
      901 ttcctacttt ggattgcgaa aaaaaggagt atatgccgaa aattcgtcag aggggtatcg
      961 aaaaaaagag atatgtctct ttaaaaaaag gtagaggtat gtacaaattt gtatatacgt
     1021 atacaaagac tacttcacag aacatatcaa agactactct cgttatggaa gaagaggata
     1081 ttaatataaa gaggatttac agtaagatta gtgttcttta tgacgcaaat aacgaagatg
     1141 gtcgaaaatt atttcaagtg tatcactatt tgagaaacgc taatgtagat acagaagata
     1201 tcagaaaaat cattgaatat ttatctgaaa atactcagta tcttattgct gagtgtatta
     1261 taaaacaaca tgaagcatgc ttttttcaaa gaaaaactga agaagggtta tacgattatg
     1321 taacatattt tttgaatgga cttcagaaaa gatataaaag tcaattttta gaaaatgtag
     1381 agcctataga tcagttcaaa aaagctttcg aaatcaaaga gttacctgaa attactatgc
     1441 ataattggtt gaaaggagaa gaatcatgat taaaaatcgc ttaatagatc aaatgtggtg
     1501 tcttgatcaa aatacagata ctctcgaatt aatcgagagt atctgcttca acacagtagt
     1561 tctcgattta agagctgaaa atgacaattg tgtaactcat atcataatta atcaaaaaat
     1621 ggctcagcag ctctctgaga gcctaagaaa atgggctgaa ggagaaaact atgaaagcaa
     1681 ttaaagaaaa aatcgttagt agaaaaataa actggttagc actcagtgta cttgttttaa
     1741 tgattagcgt tgtttttaca agtaatattg ttgaagctag cattttcaat tggtttgatg
     1801 acacaaaaag tcaaacagag tttttgaata attctcttta tagaccttac ctaaaaaaat
     1861 acaatggaga tttccttagt cagttcggga tttggcttgg atgggctgtg gtaaaaggca
     1921 tgtttactgt gactgattcg attcaaaata tgattccaga tgtgttggat ttgttcaatt
     1981 tcattgaaag tacagggcta aataatgttt atcaatcagt cataaataca attgtcgttg
     2041 gattaatgat cctttcattg atgtttgttg gctacaagat gattactgga aaaggaacaa
     2101 tagatttaag atctgttgga atgaatatag tgatgtctgt agcgttaatt cttttaatgc
     2161 caacaatgat ttctagcggt attcaatttg ccaaaatatt ttacaatgat gctactacga
     2221 ttacaaattc agatgacgga gtcgcttggt cattgataaa acaaggagta acagatctgg
     2281 catatattaa taaaacagat caatactcaa gcattgataa aacggaggat cgaaataagt
     2341 taacacgaaa aaattttcaa cagacagatt taacacaagt gttgacggat aaagtgattg
     2401 ataaattgga aaaagaaaat ccagctgcgg ataatcttcg ctatgaatta gttgaaaatt
     2461 ctaataatga atttgttgcc acaaagtttt ctgataactt cttatcaaca ttttctgata
     2521 gcttgaaatc aggttattat cgatatcaag caaatctttg ggggatttcg attgggttga
     2581 cagcgttggc aatcgcctat gtgttcagcg cgtttgtgat cattacagca atattagaac
     2641 ttgcatttaa acgtgtgtta ggtgttcttg tttttgcgac agatattgaa acaggacaac
     2701 gttcaaaagt tgttttatcc gatattttac aatgttactt aacagttgga tttcaagggt
     2761 ttggactatc tatgtttgct atgtttatca attttttaaa tgctggacaa ggtatctcca
     2821 caaatatttt tataaaaaca attgcttata tctgtgctgt gtttgtgctg ataaaaggca
     2881 gtggaacagt catgagatac tttggtgttg atattggact caaagaaggc tatgggcaac
     2941 ttgcttctgc ttttggtatg ggcgcaatgc tgtttagaaa aggatcaact ggatttaatc
     3001 gtgctaaagg aagcggaaat ggaaatggat cagacagtgg agaaggagaa gatcgaaaac
     3061 cagaaaagaa tttcggcgaa actctatcaa aaaaagctgg caaaactgga agagctttgg
     3121 gatatgccca tgaacgtgga ttatcaggtt tagcttcaga tggagctaca atggctagtg
     3181 aaagagcaat aaaaccattt aagagtatgc gtgatatggc taatgatacc aagaataagt
     3241 tcaaagaagg tttagatgat ggtacagtgt ccgctattaa taaaaatagc aaacctatgt
     3301 tagctaaaaa taaagaagat gaaactggaa aatatgctga ttccatgcca acacgtcttt
     3361 cggatagaaa agatggtgct aaggttgaaa atgccgatag aattatgtct agttctgaac
     3421 gtatgcgtga agcaatgaaa aataatgctg aatcgaataa taatcctgta agtgcaattc
     3481 agcaaaaagt tcaacaagat attgaagaac gtaaaaatgc tatgcacggt caagctaagt
     3541 ctgctgaaga attaattaat cagaagcgac aagaagctaa atacacgcct gaagcaatga
     3601 atcgtgagga aatgttaagg aaacgtgtag aagggcgtac aggagctaat acggacgaaa
     3661 aagaagcgtt gaccaaagaa agaatccaag aagctaaaaa ctcaaatacc ggattagaaa
     3721 aattggtcaa agaaaatcta caaaattcta caagtagtca aggtggccga tcagtagatg
     3781 tccgagaaaa tattcaaggt tcattgaatg gtcatgtcgg acggaatgtt gatgtaagag
     3841 agaatttaca aagctcttca aatggtcaag gtactaagac aattgatgtt cgagaaaatg
     3901 tacaaaattc tacaagtagt caagggttta aatcaatgga tgtaagagag aatgtacaaa
     3961 attctacaag tagtcaaggg tttaaatcaa tggatgtaag agagaatgta caaagttcta
     4021 caagtagtca aggattcaaa acagtagatg ttcgggagaa cgttcaaaaa gatagtggag
     4081 aaatgaaaga aaaaacacaa aaaatcaata tcgttgaaga cagaaagtca gcgaaaaaat
     4141 ttgattcaaa tcatgaaaca gtgattattg atagtgaaat ccgtgaaaat gataagggat
     4201 caaaaccaag acgaagattt acctatgaag acaacgagtt attcagagat acacttaatg
     4261 atcctaatcc gcttttcgat aatctattaa aaaaataaaa gggggaattg tcatgaaatt
     4321 aattctaaag attagtacgc atgatttctt agaacttgac gaattatgtg aatcagtaaa
     4381 gaacgcaaca aatgaaaaaa tcacggtcac taattatatt gaactgatga ttcaagaaga
     4441 attaaagatg aaacatgaga taaaaaacaa cttgaaatat tgttatctag aaaaaagtga
     4501 agagataatc catgaattaa atggctttta aaaattgtat ttgacatgaa tttacgacaa
     4561 ggaattagtg gggattaagc gattattctc ttccacttta gagaaataat gggttaaaaa
     4621 gccagaatac gtagagcaat attgacaacc tataaaggag ctattgttga aaattgaagt
     4681 aacaaaaaga taaagggagg tttacatcta tcagattaaa agtgatagta cacccatgaa
     4741 acctgcaaaa gagttgaacc taatgtatat cggtttaact ctttttattt gcaaattaaa
     4801 aaaatatcac cgaagtgacg aatgaaacta tgaccaaatt aagcaagaaa atatatcttg
     4861 caaataaagt agtataaaaa gatattgata aagccaatca aa
//
LOCUS       DAZBTF010000131         4801 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000131 DAZBTF010000000
VERSION     DAZBTF010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.137"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(82..1890)
                     /gene="pepF"
                     /locus_tag="ACHGNS_002830"
     CDS             complement(82..1890)
                     /gene="pepF"
                     /locus_tag="ACHGNS_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFN6938690.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2066..3064)
                     /locus_tag="ACHGNS_002831"
     CDS             complement(2066..3064)
                     /locus_tag="ACHGNS_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFN6938691.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3290..3943)
                     /locus_tag="ACHGNS_002832"
     CDS             complement(3290..3943)
                     /locus_tag="ACHGNS_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFN6938692.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="ACHGNS_002833"
     CDS             complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="ACHGNS_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFN6938693.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1410 a         1007 c          744 g         1640 t
ORIGIN      
        1 taaaaaaata cgtcgactcc cttacaagag agccgacgta tttttttcgt ttctattcga
       61 gaaaatcaaa aatagaaagc cttataaacg atcgatcaat tcttctaatt catttagacg
      121 ttgttcaaac atcgacattg catcttcaat atatgcagcc tgtgtcatat caactcctgc
      181 ttttttcatt acttccacag gataatcgct gttacctgct tttaggtaag ccaagtagtt
      241 ctctagtgcc tctggttcct ggtttagtat tttcttcgcc agtgctgaag cagcagaaaa
      301 gccagtcgag tattggaaaa catagtaatt ataataaaaa tgcggtatcc gtgaccattc
      361 gaatttaatt tctgggtctt cttcgactgc tggaccataa tattttgcat tcaatttacc
      421 ataactatca cttaggtatt cactagtcaa tggcacacct ttttcatctt ccgtatgcat
      481 gaaatgctcg aattcagcga attgtgtctg gcggaaaact gttcctttaa acccatccaa
      541 gtaatggttg agtacataag cccgtacacg agggtctttt tctgtttcca ataaatactc
      601 cgttaggata ttttcattcg ttgtcgaagc aatttctgct aaaaagatgg agtagtcgcc
      661 gtacacataa ggctggttcg aacgagtgaa ataactatga acactatgtc ccatttcgtg
      721 gacaagcgta aatagctgat ccagcgtatc atgccaattc aataaaatat atggatttgt
      781 gtcatagctt cccgaagaat aagcaccgct tcgtttccct ttattttcga caacatcgat
      841 ccaacgttca gagaatgctt tttctacgat ggccatgtat tcttcaccca ttggtttcag
      901 tgcttctaaa gctttttctt ttgcttcttc gtacgtaaag gtaattggag cttcccctaa
      961 gaccggtgta taaagatcat acatgtgcag tttttccact tctaataaac gtttccgtaa
     1021 ttccatgtat cgatgtaaca aaggcaaatg cttgtttacc acgtctacca aagtatcgta
     1081 tacactttca ggaatatgat tattgctcaa agacgcttct ctggcagagc tgtaattacg
     1141 gactttcgct ttaaaattat gtccttttat atgtgtgcct aaagtagaag caaatgtatt
     1201 tctaaattgt tcgtaaacac tgtacaaccc cttaaatgct gcttcgcgca cccttcgatc
     1261 cgtgctttct agcaactgac catacacgcc atgagataat tggactattt caccattttc
     1321 tccttcaatc gttggaaaaa ctagatctgc attattcaaa acagcaaatg tatcacttga
     1381 agcatcaaag atttcacctg ctccagcaag aagagattcc tgctcagcag ataagacatg
     1441 ggctcgatta tctactattt gctggatata atggcgatag acttccaatt ttggttcttc
     1501 tttgaaatat tgccaaatct ggtcatctga caattgcaat atttctggtt caaaccagga
     1561 aacggcttca ctaactttcg aaaacagact gcttgctctt gcataaagcg cttggtaatc
     1621 tgtatttcca gtgtcttgat cgtttttaag gtgcgcatat acataaatga cttcagtttg
     1681 gcgataaacc ctcaatacga attcaatcgc atttaaaaat tgagaagcgc cttgatcaag
     1741 tgttcctttg tgtttttcag attgttttaa ctcttctgat aattccaaat atttctcatc
     1801 aaactcttgg tcgcttgaaa agatcttggt caagtcccaa gttaaatttt caggcaattc
     1861 ttctcgtttt ggcaactgct ttacttccat gaaatccact ccttttcttc ccttagctct
     1921 atctatcaaa atagatcata gtctaacatc cacatgtcac agcatctaac ttttctactc
     1981 attcatgtgt ctgcttccat caattttagc ataactaaat cttgttttac atagaaaacc
     2041 gattcctagc ctatttttat acttactatt ttttcaaaga agacaagtgt atgcattcgt
     2101 taaaaaactg ctgatagacc atctcttcat caatcatagg aaaaagccat tcccttttat
     2161 gttcttttat ttggcaaagc catgagttga aatcaggaac tttctggttt tccaaggctt
     2221 cttcgtacaa cattcgataa agaaaaacct gttcttgtaa ataaaagaaa aaccgacttt
     2281 tttgatagat ccacgatgat agatgcaaaa gatgtttatg acgcaagtag cattgttctt
     2341 gtatggatac tgtattcttt tgcttgtgaa taagttggcg tgtcagccat ttcttcccat
     2401 cttcactcac tttttctatt tttttcattt ggttcatttt aatttcgtta ctagtgaata
     2461 tttctttaag gtccctacta gaaaaagtcc agcagctact gcaaaaggaa atatgccctg
     2521 atacagtctc tgtaatatga tgatacaaat aaagcttttg tttcttgaga tcagctttcc
     2581 agcaatgcac tcctcttttt ctattatagt agcagcaact tttttctatc aatgagaatt
     2641 gcgattgtcc aagaaaagaa ttaccaagta tccaccatac ttggtaactg ttattcaaat
     2701 aattttgggt tctttcaata aatctctggt gagacagtct agaacattgg atttctattg
     2761 ctagattcgc caacaaaata tccggccttt gccgcaacct cggcaaatag gcttccaaaa
     2821 atactggttc tactgactgt cccagccaat ccatgaagca ttcctttaac tgtaagtgct
     2881 cttttgattc tctttcacta aagaccgaac agttatctga tttttgatgg gcaaaatgag
     2941 cacatttttt ctttcccttt ttcaaaatca ctctttttcg acagccacga caataatatt
     3001 tctttccttt ttctgcatgt ttagcaaaac agcacttatt atattcattc attgcaagca
     3061 gcatttttct ccctctgaac ttatttacgt ctaagaattt atttacacga aaaaagaggg
     3121 cgtgaaaaag ctattctgca tcaagtaata agaacagcta aacaaaaata gttcccaata
     3181 tttttcgttt ggttgggctt gttaccgcag atgcaagctt ttgaacacat ttatacgaaa
     3241 aaaagactgc gacacaattt ttgtcacagt ctcttactat aatgctgatt tatacaaaat
     3301 aaaatttcgt tcgctcgata gcattacgcg ccatcagaca ttgtccatat tcattcaatg
     3361 tttctggtgt acgatcgctc ttttcagcaa actccaaaat tcgagcaatc tgattctcta
     3421 catccgcttg gttgagattt tccatccaga agtgaacgac taggtaatag cgctcttcga
     3481 ataaatacaa atcagtccaa gcagactcta gttcgacatc tgttgcaagt tggatcactt
     3541 gttcaaaatc ttcaaaagca aaagttgctt gtctttcctg ttcttctagg gcgcttccag
     3601 ataattcttc ctccggatcg ctgccaataa tctgctgacg aagaaagtca ctcacatctt
     3661 ctgaatcaac agtagaatct tctgattgag aaatttcttc atccggaaga tttttactga
     3721 taaacagttc taatccgtct ccttttggca aaacttggaa agtgacagct tcgcttcctc
     3781 ggaactcctc ttcgatatca acttcttcca aaatactata aaagaaattt tcgatttctt
     3841 tgtgattgcc aagtaaatcc aaaaatgaaa cgccccgttc ctctaaatct tcatgaccaa
     3901 ttagtacacg gatcgtattt tcattaatat gttccatttc cattttgcta cacctcactt
     3961 tgccacaatg tttaatatag attcattgta gcgaaatcag cttaaatgta aagaaaaaaa
     4021 atctcgctgt taagcgaatt taataaaacc agcggatttt tcaacctttc aagctatctt
     4081 atttaactat aacatttttg gcagacgaat actatatagc cgacttttca gctaattttc
     4141 cctctctatt tttcgtgcaa tcatcatctt ctactacctt tttatacatc taatcaagaa
     4201 tactttttgt aaaaacaaaa aaacttggaa aaactatctt tgtaatagtc ttttccaagt
     4261 ttcgtattat ccttctataa tccagccatc aattgtgctt gacgtaattc taattgtcgt
     4321 acatcgcgcg gtaggaaccg acgaatttca tcttcattaa aaccaacttg cagacgttta
     4381 tcatcaatca tgattggacg gcgaagcaat ccaggattgt tttgcacaag ttccaataat
     4441 tcttgtaacg gcagatcatc taaatccatg ttcagttttt ggaatacttt ggaacgtgtc
     4501 gagatgattt cttctgttcc gtcttctgtc atttgtaaaa tcgcttttaa ttcagaactg
     4561 ttcaatggtt ctgaaaaaat attacgttca acaaatggaa tctgatgttc ttgcaaccac
     4621 gcacgagcct ttctgcagga agtacaactt ggggaagtat ataatgttaa caacgtgcac
     4681 cactcctttt cgtgataaga atcacttttt tgtaacagaa gatcataata atctcccatc
     4741 tgtttctatt atacataatc aacttttaaa tagctacctt tttttcataa tttttttaaa
     4801 a
//
LOCUS       DAZBTF010000132         4587 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000132 DAZBTF010000000
VERSION     DAZBTF010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.138"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            186..2855
                     /locus_tag="ACHGNS_002834"
     CDS             186..2855
                     /locus_tag="ACHGNS_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFN6938694.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2946..3560)
                     /locus_tag="ACHGNS_002835"
     CDS             complement(2946..3560)
                     /locus_tag="ACHGNS_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN6938695.1"
                     /translation="MINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKAMH
                     MVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPGIE
                     KRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTIIK
                     ITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3680..4219
                     /locus_tag="ACHGNS_002836"
     CDS             3680..4219
                     /locus_tag="ACHGNS_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938696.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGEMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4216..4554
                     /locus_tag="ACHGNS_002837"
     CDS             4216..4554
                     /locus_tag="ACHGNS_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HFN6938697.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1420 a          790 c          859 g         1518 t
ORIGIN      
        1 atttttaagg gtgggacttt tgattcctac cctttttttg cgtttatttt tgttatacga
       61 acaaattatg aagttattta ttctaaattt tatttttttg tgaaaattag actcttttta
      121 agcagagaaa aacaaggata tgataaactg aatacaactt catagaaatt tggtggaaaa
      181 taaatatgaa aaaaaattta aagtcattct ttagagaaaa cggacgctac gcagcagcta
      241 gtttcctgat tccgtttttg attttagcac ttgtttatct aagtatcggg atctatcctg
      301 gaagcagcag aagtattttg gcaagtgatg ccttttcaca gttttctaat tttcatgcca
      361 gcttccgaaa tgttcttctt ggaaagcaaa gtatttttta tacatggaat gcttctctag
      421 gattgaatta tttatcttta atttcttact atctaggcgg gatcttcacg ccactggtgt
      481 tcttttttcc aaaccagatg atgccagatg cactgtatgt tctgactttg cttaaggtcg
      541 gctgtgccgg attggctttt tggtttcttg cacgtacgtt caagattcct cgctgggggc
      601 atgttgcact tagcgtcagt tacgcattga tctcatttat cactgcacat tcggagatca
      661 tcatgtggct ggatgcattt gtttatcttc cattagtgat tttggggatt catcgtgtta
      721 tggatcttaa acggccgaaa cttttattta tcagttattt actcttgttt ttatccagtt
      781 tttatatggg ttttatgatt ggcgtatttt ctttcttata ttttatcgcc cgtctattga
      841 caaactggtc agaatataaa aaaacgattg ttccttatgg tatcacttct ttactggcag
      901 gcggcgcttc aatgatcatt gtattgccag cagtgctgga tcttcggtca aatggcgagg
      961 cactgagtca gatcactacg ttgaaaacag aagcgacttc ttatctggat ctaatcatga
     1021 aaaatatgat tggtgtttat gacacgacaa aatacggatc gattccgttc gtttacgcag
     1081 gtctgctacc attagtcctc tgtctgtttt attttgccag tcggaaagta ccgttgaaaa
     1141 ataaattttt gtacggcagt cttttcgcca tacttatcgc aagtttctat cttgttccat
     1201 tgaatttgtt ctggcatggt atgcatgcac cgaatatgtt tttattccgt tatgcctatc
     1261 tgttctcttt cttagtgctc ctgcttgcag gaaaaggatg ggaaaagata gaacaagacg
     1321 atcgtgggct gttgttagga atcattttgg tcgtagctgc tctctttgcg gtggcatggg
     1381 gcatcaaacc tgaaggaagc tacacctatg tgacgatgac ttcctttgta ttgacagtgg
     1441 ttttcttagg gatttatgct tttgctgtgg gattttatca gcataaaaaa ctttcattca
     1501 atcatctgag tatattgtta ttattgatca tgtctgctga agcactggtc aatacgaaca
     1561 gtatgatcca tggcattctt gatgattgga attatgcttc ccgcagttta tatactgatc
     1621 cttatcctga cttgaaagct cttgtaaatg aaacaaaaaa agattccgac tcgttttacc
     1681 gcttggaaaa tctggatcca gtatctgcaa acgattcgat caactatggt tacagtggta
     1741 tcagtttgtt ttcttctata agaaatcgta attcttcctc ttacctggat aaattaggtt
     1801 tccgttcaag agggacaaat ttgaatatcc gttataacaa taatactttg ctaatggatg
     1861 ggtttacagg aatcaaatat aacatttcta aaaacgaaag cacgttcagt aaatatggct
     1921 tccgccaagc tggtcaaagt ggcgactata cactgtatca gaatgtcaat gcattgccac
     1981 tagcatttac tgcaccgttg tccgttaatg atgttgagca gccagtgaca gataatttga
     2041 ccagtcagac aaacttgatc aatacattgt ctggtttaaa tgaacagtat tttacattct
     2101 atacacctac tttgaagaat cagttgaata cgacgatcac aaagacaacg actggcgtca
     2161 cttactcaga aacagcagat aaccagccaa aagagctcac ttgggaagtc catgtgcctg
     2221 caaatacaca agcttactta agtttgttcc caaccaattt tgcacaatta gaaagttcta
     2281 gtgcgacgat cactgtcaat ggtacatcgc gaaaaacgca aatcaacatt tctggtcaat
     2341 actatgatct tggctattat cctcaagaga caacagtgac atttactgca agtttttatg
     2401 gaacgaaaga agtaagtttc atggagccta aagtcgttgg tctagatgtt gtagcctatc
     2461 aagccgcaat gaatcagatc aaagaaaaag gtgtcgaaat ggaagccact ggcagaaaag
     2521 cttctgggac tgtaaatacc gctgaagatt ccatgttggt gacaactatt ccttatgaca
     2581 aaggatggaa agctaaaatt gatgggaaac cagtcaaggt cagcaatttc aaagacgcat
     2641 tcttgatgat aaaagttcca gcaggtaaac atactgtcac tttcagttac ttgcctgaag
     2701 gatttacgat aggtgcaatc ctttttgtcc tatgtattgc attgtttatt ctttatgtct
     2761 ggtaccttga ccgcaatcgc cccttttatg gcacgattga tccagcaata gaagaaaaac
     2821 cgcgaaatac acacagaaga agacgaagaa aataaagaaa cgatggaatc tgtgtcactc
     2881 ttctatttaa atggcaaaaa tttcaatctt taaatgacaa aaaccacctg atttttggtg
     2941 gttttttata tacctatgac taaactatct cttgctgctt cgacaacttc tacagtgatc
     3001 ttatccattt gattgattct taaaatccga tcaatctgcg caaataaacg atgaataagc
     3061 ctaaaatttc cttttgtgat cttaatgata gttgtgatag cctcataatc agaaaaatct
     3121 tctagcttga tatcaattcc taaatctgcc catttgtatt ccaaaatatg atgtgtttcg
     3181 tccttactaa ggttatcaaa ttcatgtgca aatcctatgc gagagtaaag ctgcggataa
     3241 cgagacaatc ttttttcaat accaggcata ccgataagaa ccattgccag atcatttcta
     3301 tcgtaaatat ctcttagctg ttctaaatgc tgaagcttta aacgatcaat ttcatcgaca
     3361 ataattaagt caattgcttc ataggtttta ggcacaggag gatcttcttg attgtatttt
     3421 agtaccatat gcattgcttt agtcagacct attctactag ttattgtgtt aatatccgaa
     3481 ctgacacgag acatcttttc agctggtaca gtataaaaaa ctttatttac acttaatatt
     3541 tcatctgttg catttatcaa gaacaagaaa aagtctgaat attcaaaaaa aataacggtg
     3601 tacattttta aaaggagatg tataatcgag atttagaaag cgttttcctc aaaatttaat
     3661 gaattaggaa gtgattacta tgaaaaaatt atttagcgca ctttttattc tgttatttgg
     3721 ctttattggt attgggactt ctggaaatat agttaaagct agcacgatgg ttgaacaaac
     3781 aagtgaaatg gcaaaaactt ttagtcaatt aacaccagaa gaacaaagtt acttcaaatc
     3841 caaaggattt agtgataaag atacttttta cactcaaaag atagagcctc aagatccgaa
     3901 aacacgtttt tctgcaaatg tattgcggtt aaccgcatct actaaaaaaa ttactaatac
     3961 gcaggggcgg acagaatata ttataacaag tcccaattcg gcgatgtatc gtatttctac
     4021 acgcttgagt ttaggtagtg caaaatcata tactcaaaca gtaaatctag ctaattctta
     4081 tggttatggt ggagaaatgt attttacatt tactgggaaa agacaatatt acagtgtaaa
     4141 attagctatg caagtttata atacatttgg tgtagggtca gtctcttgca ctgcgggagg
     4201 attgacaata ggaaaatgat aatttcttac tatgtcttaa atagaagtga tgatgaagga
     4261 ctcttcgttt ttcaatcatt acatgaggat acgatgctaa aagagtttta taatcggatt
     4321 aagaataaac agatagaaac aaatttatat tttttaagtg actctatctt gccttttatt
     4381 agcaacccta aaattcttaa cttgttgcag acaaaagatt tgaaaagttt accgattatt
     4441 gtcattgatg aggaaattgt aaaatatggc gcttatctaa gcgtagaaga attagaaaaa
     4501 ttaactaaca ttggaatgag tattcaggag gataatgggg tacatggaac atagaatgaa
     4561 tatgcacagt tgagaacgga atttgac
//
LOCUS       DAZBTF010000133         4461 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000133 DAZBTF010000000
VERSION     DAZBTF010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.139"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            574..1008
                     /gene="bacA"
                     /locus_tag="ACHGNS_002838"
     CDS             574..1008
                     /gene="bacA"
                     /locus_tag="ACHGNS_002838"
                     /inference="COORDINATES: protein motif:HMM:NF041453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="HFN6938698.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2298..3221
                     /locus_tag="ACHGNS_002839"
     CDS             2298..3221
                     /locus_tag="ACHGNS_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFN6938699.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3237..3776
                     /locus_tag="ACHGNS_002840"
     CDS             3237..3776
                     /locus_tag="ACHGNS_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFN6938700.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1664 a          668 c          758 g         1371 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacagagaa cttaaattga attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagaaacg gataaataat aaaaaagact
      181 gctcttccga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      241 tataaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacaatcca gtaaaaaatg
      301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
      361 gagcttttta cctagaccaa ttaaaaatca caaagtgagc ccctgtatcg gttgatacaa
      421 gggctacttg gcatactcga cattatgtct actaaccttt aaaataacac gttttataaa
      481 ataaaccctc ttgacaatat ttaattttct gattattcta aatatataca gaaagcgctt
      541 tcctaggatt ttatatctaa ggaggaagta acaatgaaaa ataaaattat taaaattatt
      601 acttgtctat ttttgttcgg tatgattgtt ggagcaagta ctccgttaaa tctgtttcct
      661 tttagtgcta gaggtataaa agtggaagct gcaagttcac gatataatca taatcatcgt
      721 ggatttactt gctataaaat gaactattat gttactaata aaatgtgtag agatttgaaa
      781 aaaaattata ataagctaaa gactcctgct caaattgcat cttttattcc tattggtgga
      841 gttggaacat gggttattac aaatgctttt agtagtgctg ttgatgcaat gaatgtcttt
      901 gtaagagctg caaatcaagg aaaaggtgta caattaacat ataatgctca ctttagcaat
      961 acaacatcat atcaatacaa tgattacgct cgttatgtaa taaaataatc agaaagtgaa
     1021 actatctcat gaataaaaag attttagctg taattatttc aataatagtt ttaatcacta
     1081 tgttcttcat atttaggcta attttcaata taagcttaca aaaaagtatt ctttatctaa
     1141 ttcctatagc atttatctta gcaattttta gaagtattta cggaaataaa aactctaaat
     1201 agctaaaaat aaatcatact aaattttaca aaaagtggct agcattttct agccactttt
     1261 tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
     1321 gcggttattt gcaccaaata cggaggtatt tggctagcaa atatagagct tgccaaaccc
     1381 ttttagtgag ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc
     1441 cgtaggacgt actgaaaggg tgggacttat gagcaaatta atgcaaaatt tggctagcaa
     1501 aaaagctaaa aaatatatgc taataatcga gcgaaataaa cactttttca agtgagcgaa
     1561 tccgccatga caaagctgaa gcagtttatt tcagctttgt catggcgtgt gccgactttt
     1621 ttaaaagtct aggcacacga gcaaatatct ttatcaagct ctaatttgcg ttttaagacg
     1681 tttgaaacgc ctaagtatat aaatatataa gaataggttt aagagtgctt aaatcgaatt
     1741 ttagagggca tttagacaat tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg
     1801 ttttaagaac aagataaggg atttatacta ggactgtctt aaaatgtctt aaacgaacgc
     1861 tcagagcttc tcaaagggtt ccttatgaac ggcttttagc aaaggagccg gaggcgactg
     1921 cgcagtataa tgtgagagca gagcgatcac acctttttag cgaaccgtag ggagcaaggt
     1981 ttggaattgg cagttttgat aaattcgcga acctgtttag agaaaaaagg agtgattttt
     2041 taggcgggaa aattagcctc ccctcttttt atatctcttt ttatatctct ttttatatct
     2101 cttttatgtc ggctgtgtcc ttactctccc aagcaattca gagttttttc aagtaggttt
     2161 tctacgcttt tcaagtaggt tttctacgct tttcaagtag gttttctacg cttttcaagt
     2221 aggttttccg acattattga gtatttttat aaagtatgtt acttttattt catactttat
     2281 aaaagtagga gaagcttatg gcaaatgaaa ttgtaaaata tgaaaataga ttaaatcata
     2341 ttcctctgag aaaatttaat agtcgtgaaa tgaacttatt tttttcaatt gcttcacgtg
     2401 tacgtgaaaa aggtgtagat gagattattt tttcatttca cgatttgaaa aaactaagcg
     2461 gttatacaga acgtggggaa caatttataa aagatcttag tagcacgtat gacaaattat
     2521 taagtattaa tgcttggaat gatgatggaa atactttaac taaatttgtt gctttcacaa
     2581 aatatagcat tatacgagat aaacaacttg ttataatttc agttaatcca gaatttaaag
     2641 gactttttaa tcaattaaag aactggacaa gatttaatct agagcaattt actaatttgg
     2701 atagcacata ttcaaaaact atgtttcgat tattaaaaca atatcgaact caaggctgga
     2761 ctgaattttc caaagaaaaa ttttttgatt tactagatat tcctaaaagt tattggaata
     2821 gtccgtcaaa tattgacaaa tttatcttaa ggccaattaa agaagaactc actcccttat
     2881 ttaaaggatt aactattcgt aaaaaatatg gtaaaggtcg aggtaaacct gttatcggtt
     2941 atcgttttac ttggaaagct gaaattaacc atgctgacga tttttccaaa gggaaacaag
     3001 aagatttacg tataaaatta tttaatattg atcacaatgg agaattaaca cgagaagaaa
     3061 aatggcgcgc taaagatcga atactaaatc ttcccttagg cactcatgaa gctgatttta
     3121 aacaacaaca acaaactgaa gaaaaacagg cagaaaaacg agaactttca aacaaactca
     3181 aacaagattt actaggaaat ttacaaaact tattcgatta agcttaagga gtatttatgg
     3241 cagataaaac aatcaaagaa cttgcggaag aattaggagt tagtaaaaca gcaattaaca
     3301 aaaaagtttc tgacaaaaat cgaaaacttt ggtttgcaaa aaacggaaac aagtttgtga
     3361 ttaacgaaac cggtcaaaaa gctataaaat caatgttttt agtcgataac gcaaacttaa
     3421 atcgaaaact agttggcgaa aaatcaaaaa ccaaaaacca taaaaacacc aaccaagttt
     3481 ccgaattaaa actcctttct ttcctagaaa aacagttaaa tcgaaaagat atacagctac
     3541 aagaaaaaga taatcaaata aaacaaatgc aaaaattact cgaccagcaa caacaactca
     3601 ctctacaagc gaaccaacaa attgagaacc tacaagaaca gctccattta acttatacag
     3661 aagaaactcc aacaaccgaa tcaactgcct tttctgaaaa aaaagcagac aatacacaac
     3721 cacttattga aaaaaagtgg tggcaaattt ggaaatctaa tagaaccaat ctctaaaatt
     3781 ttctagaagt tagttcggat agtagaggaa aaagttgtac tacctgatgg ggagatcact
     3841 caaattttta tgagaaagaa aaaatagttt acaactgtaa acaatcaatg tttatagttg
     3901 taaactatca aactatatgg tgacttattg cttaaaatag gttgaaatct cactcaattt
     3961 ttggtggaaa aacaaaataa attctaatcg attttgttga aaaagtcata aaaaaacaaa
     4021 tgtgatttta acaaaatttc aaaaaaatcg tttgaaattt cgtttatttt ttttgagaat
     4081 tttgatcatc gtagggtcat tgtagaatca ttatgagatc atcgtgggat catcgaataa
     4141 agtatgggaa aggcttgcgg tgcttggtat taagggcatt ctccgcctcc caggttcttg
     4201 accattttct cttataagtg ggatttttac cacgttgaga gaaaatggtc aagaacctgg
     4261 gaggcggaga aaacatacca agcaccacaa gcaaaaaaag ttagagtaac ttccaaaaaa
     4321 gaaaaatttt ttagaacaac ttttgaaaat ttaattttga tttatatccg ttcaatttaa
     4381 acatttgata aactgaaggc tagtatttaa aatgtgtata caagtggtag cgttaaaaaa
     4441 agtggaggta taacctatgt t
//
LOCUS       DAZBTF010000134         4365 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000134 DAZBTF010000000
VERSION     DAZBTF010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.140"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            152..1075
                     /locus_tag="ACHGNS_002841"
     CDS             152..1075
                     /locus_tag="ACHGNS_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFN6938701.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            1081..2334
                     /locus_tag="ACHGNS_002842"
     CDS             1081..2334
                     /locus_tag="ACHGNS_002842"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFN6938702.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            2468..3814
                     /gene="gor"
                     /locus_tag="ACHGNS_002843"
     CDS             2468..3814
                     /gene="gor"
                     /locus_tag="ACHGNS_002843"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFN6938703.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGIGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
BASE COUNT         1504 a          690 c          939 g         1232 t
ORIGIN      
        1 tcacaatctc tttttatatt tggacagaat atatcgaact ttattttgat tttttgtgaa
       61 ataattttca aaaatccgac tttaaataga agaagtattg gaaagttttt agagctatgc
      121 tactattatg aagttgtagg aggagaaaat tatgatcgaa ctcataacca cagtagaaac
      181 tattgaacaa gcagaacagt tgcttgctgc aggtgcagat acactatatt tcggtgaaga
      241 gcaatttgga ttgcgtctgc ctgcttcatt tacacgagaa gaacagcgac aattggtaga
      301 attagcacac aagtatggta aaaaagcgaa tattgctgta aatgggatca ttcatcctga
      361 aaaaatgaaa aatgtacctg aatatttggc ttttttaaaa gaaataaacg tagatgtgat
      421 cacagttggt gatacaggta ttatttatac gatgaaaaaa aatcctgaat tgcgtatacc
      481 gtatatttat gatgctgaaa cattagtaac gagcgcacgt caaatcaatt tctgggcgaa
      541 aaaaggcgca gttggagcag tacttgctcg tgaagttcct tttgaagaga tgaaggcgat
      601 ggaagaaaaa ctcgatattc ctgtagaaac ccttgtgtat ggtgctacat gcatccatca
      661 atccaaacgt ccactgctac agaattacta taactatacg aaacaagacg aacaaaaaga
      721 tcgtgaaaga ggtcttttta tctcggaacc taaaaaagaa gagacacatt attctattta
      781 tgaagatagt catggcacac atatttttgc cagcaatgat ctgaatctct cgaatgaatt
      841 gaatgaactt tatcagcatc attactgtac atggaaattg gacggattat ttactccagg
      901 cgaaaaattc gtaaacatca ctcgactatt cgccgaagca aaaaaacaaa ttttagaagg
      961 tagctgggat caagaaaaag cggaaaaaat gactagcgaa atcagtaaat tgcatcctga
     1021 aaaccgtgaa ttagatacag gattctttta tattgatcct tctgctatca aataaggagt
     1081 atgatgaaag aaatgacagt aaaaagaaaa ttgaaacgac cagaaatcct agcgccagca
     1141 ggaacactgg aaaagttaaa aaccgcgatc cattatggag cagatgcagt atatattggc
     1201 gggaatgcat atggtttaag aagccgtgca ggtaatttta cttatgaaga aatggctgaa
     1261 ggagttgctt ttgcaaaaga acatgatgcg aaggtatacg ttgctgctaa tatggtcacc
     1321 catgaaggaa atgaagatgg agcaggggaa tttttccgca gtttgcgtga tgtcggtatt
     1381 tctgcagtga ttgtttctga tccagctttg atcgaaattt gtgcgacaga agccccaggt
     1441 ctgccgatcc atttatccac tcaagcttca gcaactaatt ttgagacatt ggaattctgg
     1501 aaagaagaag ggttggaacg tgtcgtttta gctcgtgaag tttcaatgga ggaagtggca
     1561 gagattcgta aaaatacgga taccgaaatc gaagcattta tccatggtgc catgtgtatc
     1621 tcttattctg gtcgatgcac attgtccaac catatgtcga tgcgggatgc aaaccgtggc
     1681 ggatgttctc aatcttgtcg ctggaagtat ggattgttcg atatgccttt tggtcaagaa
     1741 cgaaacagtt taactgatac aggcgaagtg gaagaagaat tctcgatgag tgcagtggat
     1801 atgtcaatga tcgaacatat tccagaattg attgaaaacg gcgtagacag cttcaagatc
     1861 gaaggaagaa tgaaatcgat ccattatgta tctacggtgt caaatgtcta taaagcggct
     1921 gtagacagtt atatggaaga tcctgaaaac tatgtctgca agcaagaatg gatcgacgag
     1981 ttatggaaag tagcacaacg agaattagct acaggttttt actacaatac accaactgaa
     2041 aacgaacaat tattcggtga acgtcgtaaa atcccagtat ataaatttgt cggtgaagta
     2101 ttatcttatg atgaggcaac acagattgct acgatacgtc agagaaactt gttttctgta
     2161 ggagatgaaa tcgaatttta tggtccagga ttcagtcatt tcagtcaaac cgttgaagtt
     2221 atgcataatg aagaaggcga atccattgac cgagcaccaa atccaatgat gattttaact
     2281 atgccagtcg cacagccggt gaaacctgga gatatgatcc gaaaacgtaa ataaggaaaa
     2341 agctgatata tccctgtttt aagcatcttc gcatacaaaa ttcaagaaaa gtgttacact
     2401 tgttttctgt agaaaaagga tcgttaaata aaggttttga ttgatctctt caataaggag
     2461 gaacaaaatg caaatatatg attacatcgt aattggtggc ggctctggcg gtattgcttc
     2521 agcaaacaga gcagggatgc atggcgcaaa agttttgctg attgaagcga atgaattggg
     2581 cggaacatgt gtcaacgtcg gttgtgtacc taaaaaagtt atgtggcaag caagtacgat
     2641 tttggaaaca attgaacgag atgcctcaag ttacggtatc caagcagatt taaaaaatgt
     2701 tgatttcgcc ggtctagtag aaagacgaga aaaatatatt gattttctac acggtgctta
     2761 tcaaagagga ttagatagca atcatgtcga ggctataaaa ggttacgcta cttttatcga
     2821 tagtcaaacg atcgaagtaa acggtgaaac ctatcgtgct cctaaaatct tgatcgctac
     2881 tggaggccgt gcaagtaaaa tgaccattcc tggtggagaa tacgcaattg attcaaatgg
     2941 attttttgaa ttaacagagt tacctaagag agcaatcgtt ttaggtgctg gatacattgc
     3001 ggctgaactt tcaggtgtat ttcgtggttt aggtagtgaa gtcatgtggg catatcgaaa
     3061 agaacgacca cttagaacgt ttgataaaat gctttcggac aatttgatcc aaatgtacca
     3121 agaagctgga ataaaaactt acgcacatca tattgctaaa gaaattacta aaaacaatga
     3181 cgaatatacg gtgctatttg aaaatggtga aaccttaact ggtgattgtg tactttttgc
     3241 cggtggacgt gtaccgaata ccgaaaaatt aggtctggaa aatacaaatg tggaattgga
     3301 caaaaaaggg tttatcaaag tagataaata ccaaaataca acggatgaac atatttttgc
     3361 tgttggtgat gtgatcggta aattagatct gactcctgtt gcaatcgcag ctggtagacg
     3421 tttatcagaa cgtttattta atggaaagaa agattcttat cttgactata aacttgttcc
     3481 gacagtcgtc tttacgcatc caccaattgc aacaattggt ttgacagaag aagaagcatt
     3541 ggaaaaatat ggcgaaaatg aattgaaagt ttatcgttca cgctttaccc caatgtactt
     3601 tgcattaaat gattatcgcc aaaaatgcga aatgaaattg atctgtgtcg gaaaagaaga
     3661 aagaatcgtc ggcttacatg ctattggtat tggtgtcgat gagatgctgc aaggttttgc
     3721 cgtggctatc aagatgggtg caactaaaga agactttgac aatacagtag ccatccatcc
     3781 aacaggagcg gaagaattcg taacgatgcg ataatttaat attaacagtt ccaacagctt
     3841 tttattgaac aaaccatgta aaaccagaaa atcagtatac tgattttctg gttttttgtt
     3901 ttttagttat ttatgtaaga ttgcttcgat aattctcaaa acgaaataag tagggaagtt
     3961 ggagattttg attatcttcg taactgacga agagtaaatg aatccatttg gtatgcactc
     4021 ttaagttcaa aatattggta tcgatgttta aaagaaatag aaatgtatcg aagaacagat
     4081 gtttaaaaaa gtgtgacttt gtataaataa attagaagaa gcaaacgagg atgttaaaag
     4141 aataccatat agttaccaaa ccaagaaagt tctcgtcaag ttcgctggta cgaatcaaat
     4201 caagtgaaaa aagggactgt gaaaaaaatt gtgtcatagt tagtcacttt ttccgaacaa
     4261 atagtgttca atcagctgtc cttcggtatt agctcaaaaa acggcaaaat atgaggaact
     4321 atttttccat ttgtttctct aatactcagg acaaaacact gattt
//
LOCUS       DAZBTF010000135         4262 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000135 DAZBTF010000000
VERSION     DAZBTF010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.142"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            54..506
                     /locus_tag="ACHGNS_002844"
     CDS             54..506
                     /locus_tag="ACHGNS_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938704.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            520..1209
                     /locus_tag="ACHGNS_002845"
     CDS             520..1209
                     /locus_tag="ACHGNS_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938705.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1334..3685
                     /locus_tag="ACHGNS_002846"
     CDS             1334..3685
                     /locus_tag="ACHGNS_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFN6938706.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3765..4160
                     /locus_tag="ACHGNS_002847"
     CDS             3765..4160
                     /locus_tag="ACHGNS_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938707.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
BASE COUNT         1505 a          534 c          861 g         1362 t
ORIGIN      
        1 tatcttatcg tagatatata ttataaattt aataagaaag cgaggtgaga atcgtgaaaa
       61 aaacgatact aagtattttt tcagtattta caatattaat tgggggctat tttcttttga
      121 atttagattc tgttaattca aacgctcaaa taaaagatca aagagtagct gaacataata
      181 ataaaaaaat gagtttgacc aaaaatatct attctatgtc agatgaagaa ttgtataaag
      241 aatttggtga tttatccggg gaacagttag aagaaagtat tccaggaatt agtgactcag
      301 ataaaattgt tgtcttgtct gctggtgggc aattaatggg agatggcaaa tccgaattag
      361 ttagttataa acaaaatcac agacagttta ttgatgttaa tactgattcc aacgctattt
      421 cgattaagga tctaaaagag cttttaaata gtaaaagaaa tgaattaaag cagataaaag
      481 aaactaattc agtggaatta gagtaaagga agattcatta tgaaaaagat ttttggtgtt
      541 tctgttgggc tacttatgat gttaacattt ggaaatgcag tatatgctgc tgagaaagaa
      601 gttatttcag atccagagca gtacgcctca cgatactttg ctgattttga agaagaagat
      661 aaagtacttg cattgccaga aggtggtttt ttaaaagggg aagctaaaat tgtatctatg
      721 gataatccag atataattca aggttcatat aattcagaaa ctgatcctgc ttcagtttca
      781 gtgacagaag caaaaaaaat tgttgctgaa aaattcaaaa atgatattga aactactcca
      841 attatgcctc gaggagcatt tccacccgat gttggtacta gcggacacct tcttgtacta
      901 gcaggaggag gaagctataa atcacagcca tttagtggtg cgggatggcg ttttggaggt
      961 atgggtgtaa aaccagctga tggtacaggc aactttttac gttggaaatc ctacattgac
     1021 gatggaagag taggaaatgt aaatcaagcc aacgcaacta aatctggttc tattcaagga
     1081 atggcattgt atgctcgtga aggattcaaa tactttgcac caaacgggcc tggttggaca
     1141 atgaatatag gtatttatta cacttataat cctgttgcag gtacgtatta tgttgttcaa
     1201 aatatttaat aaaacagcac aaagagattt ctttgtgctg ttttattatt tttagttgtt
     1261 gacaaacact aaaatataaa atataatcaa cacataaacg aacgtatgtt cggcaagaaa
     1321 gcaggggatt tttatggatg agatggtttt aagtactcag aaatggctaa acaagaaata
     1381 cagtaatgtt acagggtttg ataaggttcc agaaaatggg cgaacagggt ggcctacaat
     1441 ctatggactt attgaaggac tacaagtaga acttggtatt actaatctag tagctaattt
     1501 tggacctacc actgaaaaaa tgtacgataa tcaggttact cctaaatggg gcaaaaatct
     1561 acctaaaaat attgtatttc ttattcaagg tgccttttgg tgtaagggga ttaatccagg
     1621 tggcttcgat ggggtatata ctccatattt agatactgct gtaaaagagt tgcagactga
     1681 tgcagggttt aatggaagcg ctgtaactgg agttttggat gcgaaatggg ccaaagcgtt
     1741 atttgatatg agtgcttttg ttcttgttcc cggcggagat agtaaaatta gaaaaatgca
     1801 gcaatggtta aatgtaaatt atttagaata cacgggaata atgccttgtg atggtattta
     1861 tcaaagaaat tcaaatcaag cattaatttt tgcattacaa gctgaactag gatattctcc
     1921 cagtgaagca acagggtctt atggtaatgg gacaactagt aaaacttatc ctgtgtctga
     1981 aggtaactcg ggaaactatg taagaataat tcaatatgga ttatatgtta atggttattt
     2041 tgaagacggt acattcgatg gtattttcac taaatatatg ggtttagaag tactggcatt
     2101 ccgaaaattt atggtattgc ctgaatatac tgaaatagca gatgttgttg taataaaagg
     2161 actattgtct agcgctggag atattagaag atctgcagat ggcgcagata cttctacgca
     2221 attaactaga tctcaaatcc aaacattggt tgataatgat ataaaaatag ttggtagata
     2281 tcttactggt actgttggaa ttggaaaaga tgaacgaaat aagtatctaa ctacagagga
     2341 attgaataat attttttcta aaaatttatc tgtgtttcct atctatcaag atggaggtgc
     2401 agcattagct tattttactt atgatcgtgg cttatcagat ggtaagaagg ctatagatgc
     2461 agcaaaaaat ctaaatatac cattaacaac agttatttat tttgctgttg atctagacat
     2521 gttaggggaa gaaattcttg catatgctgg agaatatttt aagggggtat ctgctgtaat
     2581 gtcatatagc gggtatcaaa cgggtgttta cggaacaaga aatgtttgtt ctcaaataat
     2641 caataacggg tatgcttcgt ttgcatttgt ttcagatatg tcgactggtt atagtgggaa
     2701 tttaggtttt cctatgccta gacaatggtc ttttgatcaa tttgttgaat ttaccatagg
     2761 aagtggcaac ggagctgttg gtatcgattt aatcgctaca tcgggtagag atagcggatt
     2821 taatgagtta tcaaatgata ctaataatga taattacatt gccaaataca atcagaaggt
     2881 tataaatcag atggtacgtg cgtatcaata tctatcacaa gctggtcttg ataatccttg
     2941 gaaccctcat ttgacatttt atagatatgt aaattattct ggtttgtcat gggacatcat
     3001 ttcttctcct gtaacagagc atgatagaaa aatttatgat gaatatagga ataaattaac
     3061 taatagcgag ggcctgtata attattttat agatcctaat actggttcaa taataggttt
     3121 gcctcactta attgtgacgt tacaatcaca gatgtttcta gccgacacaa tcaatgatag
     3181 tgtttctgac tttgcaggtt ggttaggaga tttaatgact tgctggggtg aagttaagaa
     3241 attaggaata caaatggagc aaggtgtatt tgcattagta ggtacggctg caggtggaac
     3301 atttagtcta gaagatttat accaagatgt tgacgcggta aatgtttata ccttagcaaa
     3361 aagaaattct gattcaacag ctttatcggc tttaaaaacg tactattgtg ataagggtgc
     3421 tattgataga tttaatcaat ttgttaagaa cagatttggt tcttcagata aaatcgcttc
     3481 agtaacaaat agtttattag aaaatgacga aacagttgat atcttaggtg tagggataat
     3541 ttcatttttc attgataaaa tagttaataa ttcacttggg acagatttta cttatcttga
     3601 ttttattgga gatgagggga aagttgcaaa tggattttct gaaaaaatta ttcaatttca
     3661 agaaaataga agtaaactta attaagaatc gtttactatt taaacagtgt ccttttcctt
     3721 tttaaatata tacgataaaa taagtatatg aattgtaaaa ggacatgatg attatggaag
     3781 aaatagaatg gaataatctg actcaaaata ataaaaataa ttctggatgt ggaattatct
     3841 ttatgtttgt tatagttttg ggaatagtag tacttttaat aactgtcaag atagtagatt
     3901 actttgcgaa tactcctgaa gaacaaatac agagaaaact tcctgagttt gaaagagtac
     3961 caccttatga tccaagagat aattcatttc aagtaaaaaa attaaaagag tctccaaatg
     4021 aaatgtggtt tgaagttaca gttgaatcaa gcgacttcaa agaaaaaaag aaagaataca
     4081 attatattgt aaaggcaaaa gtatcaaaaa gatttatttt taaccctaaa ttagaaatat
     4141 atagtgttgt aatagaataa tagtgttaaa aaaattgtaa aagttaaaga agtcaaattc
     4201 tgatgtgaaa aataaaatat accatggaag acataaaaga aaaactggat aactagttta
     4261 tt
//
LOCUS       DAZBTF010000136         4241 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000136 DAZBTF010000000
VERSION     DAZBTF010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.143"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            45..746
                     /locus_tag="ACHGNS_002848"
     CDS             45..746
                     /locus_tag="ACHGNS_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFN6938708.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1318..2334)
                     /gene="tsaD"
                     /locus_tag="ACHGNS_002849"
     CDS             complement(1318..2334)
                     /gene="tsaD"
                     /locus_tag="ACHGNS_002849"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFN6938709.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2331..2795)
                     /gene="rimI"
                     /locus_tag="ACHGNS_002850"
     CDS             complement(2331..2795)
                     /gene="rimI"
                     /locus_tag="ACHGNS_002850"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFN6938710.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2802..3344)
                     /gene="rimI"
                     /locus_tag="ACHGNS_002851"
     CDS             complement(2802..3344)
                     /gene="rimI"
                     /locus_tag="ACHGNS_002851"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFN6938711.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3328..4050)
                     /gene="tsaB"
                     /locus_tag="ACHGNS_002852"
     CDS             complement(3328..4050)
                     /gene="tsaB"
                     /locus_tag="ACHGNS_002852"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFN6938712.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
BASE COUNT         1249 a          911 c          735 g         1346 t
ORIGIN      
        1 aaagtgacaa taaatatagt gaacaataag gagaggactt tttaatgcgt gttaataaaa
       61 tcgattttca cttttcaaaa ctcagatatc aacctgattt tgaacatttc tcggatgaag
      121 agttccaaca gatcaaggaa catactgttc ttcgttctta taaaaaagga cagatccttt
      181 tcgacgatgg tgatcccaga caacggttgt atatcttgac caaaggactc gttcgtttag
      241 aacgttatga tgaaagtgca acttattact actatgacta tgtgaataca aataaccttt
      301 ttcctatgtc aggaatgttt ggagaagaga cctacactta tacagctcaa gcgatgactg
      361 atattgaaac attctacctt cctgtttcct actatgaaaa attagcacga aaaaatcccg
      421 aacagttgat ctatatgata caaaaaattt ctaaagtagt tgaaatacat gaattgcgta
      481 tccaaatcgg attgacctct agcgcctttg accgggtaaa acaaaattta ttcattttga
      541 aagaagaatt gggcgtacca acaaaagatg gtcatatcac cattccttat ccgatcacct
      601 taaaagaatt agcagtgaat agcggcacga cacgtgaaac tgctggacaa gtaatcaaac
      661 aactacgtga ttccggactg ttagattatg acaaaaagcg ttttactttc tgtaagacag
      721 ctactactcc agctatgaga gaataacgcg aacaaaaata agcagtgacc ctgatattta
      781 tcagggtcac tgcttatttt cctccctaaa acttctgaaa gatccggcag ttttagatca
      841 ctgggcaaga ctcaaagatt ttctatgtgt gtttcagtta tgtaatattc actgtttgca
      901 aaaaatactg atagattaac tagaggaagc gtcttgtcct catccgacct agattattcg
      961 atattcctcg acaataaacg attaatcagt gagggcaatc agaggaacgg ataatgatga
     1021 ataagctgta aaaaataggg ctttgacaaa agcgtgtcac agcccttatt tcgattaaac
     1081 ggtgtgtacc tgtcaactct tcggtcacaa gtcataatgt tcataaacat gaggagctgt
     1141 tttttgaaat tgttccttgt gaatcagagt taaacgacag gcttacgaac tcttgattaa
     1201 acggcgttca aaagcttgca tacgcggtaa taagaacagc caggtcaaaa acatgagaag
     1261 ccgtttttcc tgggctgttc ttattacttg atgcagggca gatttttcac aacctcttta
     1321 ttcagataaa accaagcttg gatcagcatt caactgatag ctagcaaaat ttcttttttg
     1381 catttccaca tgtgctgctg ctccaatcat tgcagcatta tctccacaaa gtcgaagagg
     1441 gggaatcact agttctacct cagggagttt agttgccaaa gcatcctgca atccttcacg
     1501 caacccttga ttggccgcta cacctccagc tacaatcagt tgcttgactg gataattttg
     1561 acaagctcga agtgttttag taatcaatac atcgatcaca cttgcttgaa agctagctgc
     1621 taaatcattt ttgtctagat cttctccacg ctgttgtgca ttatgcacca aattgataaa
     1681 tgcacttttt aatccgctaa aactgaaatc ataattatct tcatggatca tcgctcgtgg
     1741 aaaatggtaa ttatcttttc cctgatgagc tagctgatcg atttcttttc cgcttgggta
     1801 agaaagacct aatactcttc caactttgtc ataagcttct cctgctgcgt catctcttgt
     1861 ctccccaatg atttcatatg atccatcttc ttgcatatag actaattctg tatgtcctcc
     1921 gctaacaaga agtgccatca atggaaactg gaatggttta accagacggg ctgcatagat
     1981 atgccctgcc atatggttta caggaataag cggcaattgg tgtgcccaag caaaagcctt
     2041 agctgcagaa ataccaatca gcaacgagcc gacaagtcct ggtccataag tgacagcgac
     2101 tgccgataaa tcttctgccg acacaccagc ttctaccagt gcatcttcca agcataaggt
     2161 gatttgttct acgtgatggc ggctagctac ttcaggaact acgccaccaa aacgtttatg
     2221 actgtttatt tgagaagcaa cgatgttaga taagatctct gttccatttc taacgaccgc
     2281 tacactcgtt tcatcacaac ttgattcaat cgccagaatc aattcttcat tcatttcatc
     2341 gtctccgttt ttaactttct gctcataatg atcgcatctt ccgtcggatt gcgataatat
     2401 gatttccgtt tacctaatat acgaaacttt tctgattcat aaagttttct tgctgcttca
     2461 ttagatagac gtacttctaa aaaaacatgc agcgaatctt ttatttgttc cgtaatcagt
     2521 aattgtaaca aatgccgtgc atgccctttt ccttgttctg aaactgcaac agcaatattt
     2581 gtgatctcga cttcatctat tactttcgag tatccaataa agccacagat ctggttatct
     2641 tcaactaaaa gcagataatc agagtgaggc tgttggatgt ctgccaaaaa ctgcattttc
     2701 gtccaaggtg aaccatgtgg ataagcattt tcgctaatca tccatacgca ttctgccaag
     2761 tacgcttcat caaaatcttt ttttctgcaa atcaactaat ctcactcatt ttctaaaagt
     2821 tttatcatat ccaacgcatc ttcttttgtt tcagtgtaat atcttttttt gatcgttgtt
     2881 gcttgaaaac ctaacttccg ataaagtctt tgtgcatctt gattactcat acgcacttct
     2941 aatgacatcg tctcacagcg attcattata gcaaagttct cgatttcgtc aataagcagc
     3001 gaaccgattc gtttacgctg atatgccggt cgaacggcaa tattcgtgat atggcaatct
     3061 aaaccgaacg ctcttgaacc gataaaacca atcattcttc cattatcaaa gatacctaga
     3121 tatagatgag gaagcggtga cttcaattct gatataaagg cactttttgt ccatggcaat
     3181 tcgccattat agacttctcg ttcgacatcc agcagctctt tgatatcttc tgtagtcaat
     3241 tctcttagta cgtaacgacg atcgatttgt tcgattgttt tttctgtata aatcatttta
     3301 tcttgaaaaa agcttctggc atacgctcta aattttttca acataacttt cgtctccagg
     3361 tgtgtgcgtc tccaaccatt tttcttctgc ttctacccgt tttaaatagc gaggaagaaa
     3421 agcttgtttc tctgcaactg gttcagctaa agcacctaat tgagccacag ttacaccact
     3481 tggaatatcc catgctggaa ctgtattaat catcgcacta ggcaatattt cctttatttc
     3541 atagaagaat ttgtgacaat cactcccgac gaaataaata gcttcattca gcttttgtag
     3601 ttgtgtgaac aaatcagaag cagaaatatg tacatcttca agtacaattt ctaatctatt
     3661 ttctttccag cggtatgccc cagcgtatac attttttctt cgagcatcaa acatgggcac
     3721 aataatttta tccacaccga tgcaattagc cgcgatggtt ttcagactag atactcctac
     3781 tagctcttta tctaatgtat aagcaatcgt ttttgctgtc gttacaccta tacgaagccc
     3841 tgtataagaa ccgggaccat cagaaaccgc gattcggtca atgtcttttg gacttatttg
     3901 aagatctgca aacaattggt cgattgcagg catcagtgtt acactgtggt tgcgtttgat
     3961 atttgtttgt atttgtccaa gaatttgttc gtcttctacc acaccgatcg ccaaagtttg
     4021 gtttgcagta tctattccta atgtaatcat atatttgatc ctttctgcta tgacattctc
     4081 gccttccatt gtagcatact tttttattct gaggatgaaa ataccaaagg caaaactagt
     4141 acaatgatac atgattatct gctggcgggc aaattttgcc ccttcctgaa acacaaaaaa
     4201 gctgcggtat cacttcattt ccccacagct tttccatctt t
//
LOCUS       DAZBTF010000137         4132 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000137 DAZBTF010000000
VERSION     DAZBTF010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.144"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..738
                     /locus_tag="ACHGNS_002853"
     CDS             <1..738
                     /locus_tag="ACHGNS_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFN6938713.1"
                     /translation="LSKTIEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLE
                     NMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQEN
                     ELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIAN
                     YFSVSETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHF
                     SRVFKKYAGVSPKQYQLGLVDNFNNIS"
     gene            755..1189
                     /locus_tag="ACHGNS_002854"
     CDS             755..1189
                     /locus_tag="ACHGNS_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938714.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            1806..>4132
                     /gene="esp"
                     /locus_tag="ACHGNS_002855"
     CDS             1806..>4132
                     /gene="esp"
                     /locus_tag="ACHGNS_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFN6938715.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         1520 a          576 c          781 g         1255 t
ORIGIN      
        1 ctaagtaaaa ctatcgagca aatcaaaata gataatctgt tatcacaaaa ttacgatcca
       61 gaaatatatc tgtttcttta tgaaaataaa atactggaat atgttaaaaa tggagatatt
      121 cgaaatttag aaaacatggt ttttaatctg agtaatggaa taattccaag tgttagtgga
      181 gatacgattc ggtctgaaaa aaattattct atcattgttt ttgaaaagtt agcacaaacg
      241 tctataacat taggaatgga tataatagaa gcgtatcaaa gtagagatgc tttgatacaa
      301 gaaaacgaat tagctgtatc gttacctgag gtcttaaaag tgagggattc tgggattgtt
      361 tattatacta aggaaatcgg aaaaactaag atagagcatc tttctccctt aatttcttct
      421 gtcgttcaat tcatcggctt aaatatctat aaaagaatta ctgtaaagga aatagctaat
      481 tatttttctg taagtgaaac taaattaaga gaaagcttta agaatgaaat gcatattacg
      541 atttataatt atatctctaa gcgaaaaatt tctacggcta aaataatgtt gaaatcaaac
      601 catactatca gtgaagtttc tttaggttta ggattttcag attcttcaca cttttcgaga
      661 gtattcaaaa agtacgcagg tgtatctcct aagcaatatc aattaggttt agtggacaat
      721 ttcaacaaca ttagctgaga ggttttattt tattatgaga ctatatcatt tacgtagttt
      781 ggaggagttg aggaaattgg attttaacaa aaaatttttt tgggaaattt ctgatttgag
      841 ttgtttcgta gaatctgagc gtctaagtaa tggtaataat agtgtgttaa atgaatatga
      901 caaagtagta attaataaag taaaaagttt atgtgaagaa gctagagaat ctatcagtca
      961 tattaatggt agcaatttta aacaaacatt atttaatctg attaagctag atgctaaaat
     1021 atcaagttat ttattctttt tgatacatga tgaatttatg gattatcaaa aattggatca
     1081 gttaattgaa agagaaagct gggaagatta ttacgtgggt ctaacttttt cagaaaagtt
     1141 aaacaactac aagttaatag attataagta tttgagtgaa agcaattaat tattcatgta
     1201 gagtactaga cactttaaat atggacaatt caattataaa gaaaaagaat caataattta
     1261 atgaggctgt gacaaatgtc attttttgtt tcgctaaaaa cgagtgaaaa aatgttgata
     1321 actccaatca ttcgtttaag attctaggaa tcattcagaa atgctgtgaa accgatactt
     1381 tataattata aagaaggtga tttcaaattc cgaagaagga atttgaagtc acctttttac
     1441 ttatgtcaca gtctcattca gtaatgcggg ataaggctcc ttattccgtt atttgaatac
     1501 tctgaagttt aatctaagaa agggtaatat tttgaaaaca tgcttcatcg catagaaatg
     1561 aacttaattt cagaaagaat acatacatat ctttttatat gttttcagct gctgaatggt
     1621 taaaaagcac aaatatccga aaaaaacatg aagaaatgta ctttttttgt tgtaatgttc
     1681 atgaaattct ctaagtaaat tagttaacat aaggttagta agattcctat tctgtgaata
     1741 atacgtatca gatttatctt ttattcttac taggaaagta attattaagg gagttgattt
     1801 gaataatggt tagcaagaat aataagagag tatttctaga aaaaacaaag aaacgagtat
     1861 tgaagtacag tatcaaaaaa ttaagtgttg gggttgcatc tgttttgatt ggtgttggtc
     1921 ttgtcttggg aacaactgaa ctagttaaag cacaagatga aatttctcca agcagcccac
     1981 ttgaaacagc gataagctcc gtacaagtag gtgacaaagt agcttcgggg aatacttttc
     2041 aggagaatcc tggttatacg aaaaattata acttttcgga tttacaattt agtcctcaag
     2101 aactaactgg agacacacta aaaggcaata caattggatt tgaagtttac ggaaaacata
     2161 atattgcagc atcaactaaa aactgggaaa tccgtcttca attagatgaa cgattagcta
     2221 aatatgtaga aaaaattcag gtcgatccaa agaaaggaat tggatcttct agacggacct
     2281 ttgtaagaat taatgattcg ctgggtagac ctacaaatat ttggaaggtc aattatattc
     2341 gtgcaagtga tggattattt gctggggcag aaacaaccga tacgcaaacc gctcctaatg
     2401 gggtaattac gtttgaaaag agcctagatg aaatttttaa agagataggc atagacaatc
     2461 tcaagaccga tcgtttaatg tatcgaatct acttggtaag tcatcaagac gatgataaaa
     2521 ttgtaccggg tattgatagt acgggctact ttttaacaga ttcagatgat ttctataata
     2581 gcttagatgt atctgaaaat aatcctgacc agtttaaaca tggatcagta agtgcaaaat
     2641 atgagaaacc taatactcaa acaaaagatg gttcaggatc cacaggtgct aatggagcta
     2701 taatcttaga tcataagtta acgaaaaatt ataatttctc atattcatct tccgcaaagg
     2761 gcacgccttg gtatgctaat tacaaaattg atgaacgatt agttccatat gtagcgggta
     2821 tacagatgca catggttcag gctgacaaag taacatatga tgtttcgttt gaatccggaa
     2881 caaaagtagc tgatttagca atcgagcgac gggaaggtca tgaaaattat ggcataggtt
     2941 caataactaa taatgattta actaaactta tcgattttgc taatgcaagt ccacgtccag
     3001 tcgttattcg atatgtttta caattgacta agccattaga tgaaatctta gaagatatga
     3061 aagcaacagc acaagttgaa gaaaataaac catttggtga agatttcatc tttgattctt
     3121 ggttgtcgga tacgaataaa aaattaatcc agaacactta tggaacaggt tattattatt
     3181 tgcaagatat tgatggtgat ggaaaccctg acgataaaga agagagcgga gacacgaatc
     3241 catatatcgg gaaacctgaa ttagaagaag tatatgatgt tgacacaaca gttaagggga
     3301 aagtattcat ccacgagtta gcgggaacag gtcacaaagc ccaacttgtt gataaagaag
     3361 gtactgtatt agcagaaaaa actatcgctc caaatgaaaa agatggggct ccaatttcag
     3421 atactgtaga atttgaattt acgggtgtag attcaagtaa attaatcgcg aaagatgaat
     3481 taaaaatcca aatcgtttct ccaggttttg ataaaccaga agaaggttca accgttatta
     3541 aggaatcacc aaaagcggtt gataaacaaa ccgtggtagt tggatttaaa ccagatgcta
     3601 aagaatcaat tcggaataat aaaaacttac ctgaagatgc agagtattca tggaaaacag
     3661 agcctgatac ttctaacgtt actgatagta cgaaaggtat tgtaactgtt aagatcggaa
     3721 atcgaacttt cgacgtggat gtagagtttg ctgtaaaagc ttctcaagct atggaaaatg
     3781 atgcaacata cgtacctata acaacaactc cagaaacgac agttcaaagt ggaaaaccta
     3841 catttgataa accagatgtt cctttagcta aagatgcctt ttcaatttca gatgtctata
     3901 ataagtactt cggtaatgca agtgttgatg caaacactgg tattgttaca ttcactccag
     3961 ctaaaggtgt aggagaatcg gagccgatta ctggaacaat tcctattaaa attgtttacc
     4021 aagatggttc tgtaggcacg accgatttag cagtaactgt aagtaaagat atttatgaaa
     4081 atccaggaga aaacattcct gcaggctacc acaaagtaac cttcaccgca gg
//
LOCUS       DAZBTF010000138         4067 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000138 DAZBTF010000000
VERSION     DAZBTF010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.145"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1157)
                     /locus_tag="ACHGNS_002856"
     CDS             complement(<1..1157)
                     /locus_tag="ACHGNS_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN6938716.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRV"
     gene            complement(1160..3409)
                     /locus_tag="ACHGNS_002857"
     CDS             complement(1160..3409)
                     /locus_tag="ACHGNS_002857"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFN6938717.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            complement(3506..4009)
                     /locus_tag="ACHGNS_002858"
     CDS             complement(3506..4009)
                     /locus_tag="ACHGNS_002858"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6938718.1"
                     /translation="MENSIYLVTEPNTQLKDQDNLVSELSRQKQVYKFLELLLNSASY
                     LNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIK
                     EYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLG
                     LGIINGE"
BASE COUNT         1311 a          744 c          580 g         1432 t
ORIGIN      
        1 actctagtat tttttttatt tgtcgcaaaa aatgttacaa ataggatcac aataaatatt
       61 cctgcgaata caaaaaatgg agcagtcaaa tttgtacttc ttgttagaaa ttgtgctaat
      121 tgcatagcaa taggagctat aaaattacca atattacagc caataaaaat caatgaagta
      181 gcaaaattaa ttgtattctt tgtagtgact ttatctaagt tactaaagat aaatgggaag
      241 caccatgatc ctgttatact tgataaaaat aaacccgtta gcaggaacgt catattaccg
      301 tttgacacag caattaacaa atttgaaatt ataaagaata taattcccaa gtatagggtt
      361 ccttttccta tccacttatt tattggtcca aacataaatc ctgctagtat tcctaagata
      421 ggcattagag ctaaatagtt actagcatta aagtttaccc ctttaatttc agttgcaatt
      481 gatgcaaatc gtacagaaat agcaatacta ttcattacga gtaagattgc aaatagaact
      541 agggcaaaaa ccattggatt catcttttct tttacatgat tttcaggtat gtcactattt
      601 tcatctctaa tttcaggaac ttttagcgcg aataagagag ctacaggaaa cgcaattgca
      661 tagactaaaa acgaagccgt ccaattaatg ttcactaaaa ctccagcaag aaaaataagg
      721 attgtctgac ctaaagcttc cattgaattt ctaataccta atagcgttgc tctagtattg
      781 ccagagtata aagagctgat atatgtcaca gccaatgaat tatatactcc taaaccggca
      841 cctaatatta ctcgactaat aagaacggtt tggaaggtag ctgtaaatac tggaataatt
      901 cctcctatac cagctaataa taaacctaaa attattgtct ttttcattcc tattcttttg
      961 gcaaagtaac tagataataa gataaacaaa ataacagtta tacttggtgc agttcctaaa
     1021 agttcacttt gactttgtga tatccccaaa gactgtttca tttgtggaat aacaccatta
     1081 attgcttgag agcttgttaa cattagtgaa atacttaaga ttgctccttt ttgaagtaag
     1141 cttttttggt tattcatctc tacttttcct cctgtgttat aacccaaatc ttggaatcaa
     1201 aatcaaatgt ttctgggcgt cgattctgat catttatttg tccactagat gcatctgaac
     1261 agactagtcc tgctctcatc aattccattc cagatcgcaa actattttgt cctacattat
     1321 aaagtccgtc attatctaac ccttttaatt tcagtcggcg gtaaggagca tttacctcat
     1381 ttaatgtttt ataatgagca acaatagccg ttttcttatc atgactaaca accatccacg
     1441 cggtttgatt atcatcatcg aatggtgata atagtctata aaaagtacca ttatgaatta
     1501 tggttctata ttttttataa aaggcaattt gttcttttac aatcctcaaa tcgccttcac
     1561 ttaattcatt taagtcaagc tcatatccaa atgcaccaaa aaaagctaca tttcctctag
     1621 tttttatagg tgttatacgg tttgtttgat gatttggaac aattgataca tgagcaccaa
     1681 tagacgaaag aggatacagc atactagttc catattgaat ttttaacctt tcaattgcat
     1741 ctgaatcatc actagcccaa gcttgaggtg catagtgaag cattccagga tcaaagcgcc
     1801 caccacctga tgaacaagac tcaaatagta tgttaggata ttttgttata agtttttcat
     1861 ataagcgata tacgcctagt acatatctgt ggaatacttc tccttgttga tctgctgaca
     1921 agtgactgtc aaatacatca gtcaatggac gattcatatc ccacttaatg taatctaagt
     1981 ttgattcatc aatgatttta cacatttgat cgaaaatatt ttcaacaacc tcatctaacc
     2041 caaaatttaa tacatattgg ttcctaccat gattaggttt tcgattaggt gtagaaataa
     2101 tccaattcgg atgtgtgcga tataactcac tatccttatt caccatttca ggttcaaacc
     2161 ataaaccaaa tttcatacca aaactacgaa ttttttctga aaggctgccg attcctctag
     2221 gtagtctttt aggatttaca tgccaatctc ctaatcctgc acaatcgttt tctctttctc
     2281 caaaccagcc atcatctaat acaaataatt caataccaac atcaaatgct tttttagcaa
     2341 tatttaccaa cttttcttcg tcaaaatcaa aatatgttgc ttcccaatta ttaattaaga
     2401 caggacggtc tttttctcgc cacttaccac gagctaagcg ttttctaaat agttgatgaa
     2461 aagtttgact catggcattt aaacccttac ttgaataaac cagaatcgct tctggtgctg
     2521 taaatatttc tcctggattg agtttccaat taaatccaaa tggattgatg ccaacttgta
     2581 gtctagtaac atcccatgta tctacttctg cttgaattaa gaagtttcct gagtaaacta
     2641 gtgctgcacc aatagcttct ccttgatttt catcagtatt ttttcttttt aaagtaacaa
     2701 atgggttatg ctgatggctt gaaatacctc gagtagattc gattgattga attccttgtt
     2761 gtaaagtacg cgacttaata gttctttctc taccccaagc accagataat tgttgccatt
     2821 cataatttgc atcgggtaaa tccaatgata aactgctaat attctcgatt atttgaatat
     2881 cttttccttt attcataact cgcgcgctac gagtaataac aggataatct ctataaattg
     2941 tatataataa ttctacttga acatttgtaa ctgaatctaa aatataaact ttcaatgttt
     3001 tgctttcatc attattatca acataggtag caggtaaacc ttctagttta ggttttcctt
     3061 cttccacttc gtacccttga tagacaaaat ctgataagcg agagccattt ttctgtagta
     3121 atgaaatagc aggatgtcta aaatctgttg taccatattc aggaaattct tgttttaagt
     3181 gctctaacga atatagcaaa tcgttttcat ttctataggt tgtcataggc cgatgatgca
     3241 tctcaattag atgactatag tcattatctg gtaagttctc accataatat aaatgcaata
     3301 atttaccatc ttccgatatc ttaaaaatgt aacttatctc ctgattaaac aaatgaaatt
     3361 gttggttttc tgatcgataa ataatattca tttcattatc cttatacata agctctcctc
     3421 cgtttcttta ttgcattata aaaaacgctc acataaatag taagcgttta aagtaacgaa
     3481 tatttatcaa aatcaaatgt ttagtttatt caccattaat gattcctaga cctaatctac
     3541 gttttcgata tactcccggt gaaacaccat ttgctttctt aaaggcttta gaaaaagatt
     3601 gtactgttga gaatcctatc ttgtcactga tttgctggat tgaatattca gttgatgata
     3661 acagatcgtt tgcaatagaa attttgattt cactaatata ctcttttatt gatattccca
     3721 tatcattctt aaacaatcgt gataaataat tttcgtgtat cgatagactg acagcaatat
     3781 ctttaacaga taatttttga tacgcattct cacgtattat ttcaattgtt tgacgaacaa
     3841 aaggattcat ctttttagta attatccctt cgttcagata actagctgaa tttaagagga
     3901 gttctaaaaa tttataaact tgtttttgtc tggacaattc actgactaga ttgtcctgat
     3961 ctttcagctg agtgttaggt tctgttacta aatatataga attttccata tccaccctcc
     4021 tatgttagat ttggataatt tttgttttat tatgtttata ttattac
//
LOCUS       DAZBTF010000139         4066 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000139 DAZBTF010000000
VERSION     DAZBTF010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.146"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..1434
                     /locus_tag="ACHGNS_002859"
     CDS             85..1434
                     /locus_tag="ACHGNS_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN6938719.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1562..2152)
                     /locus_tag="ACHGNS_002860"
     CDS             complement(1562..2152)
                     /locus_tag="ACHGNS_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFN6938720.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2299..3651
                     /gene="brnQ"
                     /locus_tag="ACHGNS_002861"
     CDS             2299..3651
                     /gene="brnQ"
                     /locus_tag="ACHGNS_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFN6938721.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1172 a          744 c          789 g         1361 t
ORIGIN      
        1 aaattttgga atcttagttg tgtttttcag aaaactctct taatatggaa gtaattaaac
       61 ttttttagtt aggatgattt aaaaatgaat gaagagcaag aactgcagcg tggactgaaa
      121 aatagacatg tccaactgat ttctattggc ggagcgattg gaacggggtt gtttttaggt
      181 tccggaaagt ctatccagct agctggccct tcgatattgc tcgcctatct tattactgga
      241 tgcatctgtt ttttcattat gcgtgcgtta ggcgaacttc tgttatcaaa caccaataac
      301 cattcttttc tggattttgt tgcagaatat cttggcaaaa aagcggcttt catcactggc
      361 tggacgtatt ggttctgctg ggtatcgatt gcaatggccg atttaacagc catcggaatg
      421 tatgtacgct actgggcgcc tggtgttcct cagtggcttc ctgaactgat tgcattaggt
      481 cttcttcttt gcttgaatat ggtggctgtc tcattgtttg gtgagttaga gttttggttt
      541 gcattgatca aagttgtagc gatcattgcc tttatcctcg taggagctta tatgatcctc
      601 acccactata cgacagatat tggcacagcc agtatcacga atctatggtc tcatggcgga
      661 ttctttccaa ctggtgcaaa aggatttatc ttagctttcc aaatggtaac ctttgctttt
      721 gctgggatag aattagttgg attagtcgct ggagaaacag aaaatcctga aaaagtgttg
      781 ccggaagcta tcaataatat tcctatcagg atcatcttat tttacttagg atctcttttt
      841 gtcattatgg caatctatcc atggaacagc ttgaatccag ataacagtcc atttgtagaa
      901 gtattttcgg aaattgggat cacgattgct gcttccttga tcaatttagt cgtattaagt
      961 gcagcagctt ctgcctgcaa cagtgcaata tatagtacag gacgtatgct tcgttcttta
     1021 gcgcaagaag gcagtgcccc taaaaaattc aaaaagttaa caacacatca tgtgccaggg
     1081 aatgctttga cattttctac tatcgtcatt ttcatttctg ttatcttgaa ttacgtgatg
     1141 cctagtgaag tattcacact agtaagcagt attgccacta cttgtttctt gtttatctgg
     1201 agtatgcttg tctacacgca catgaaatat cgtaaaagta tcctcggcaa aaaagctcat
     1261 tcatttaaaa tgccgttgta cccatttagt aattatctgg tatttcttta catggccttt
     1321 gtgtgtgtcg tcttgttcct aggaaaagat acgagaatcg ctttattact aacacctgtg
     1381 tggttcttgc ttctattact aatctatcat atgaaatatg aacgtaagaa gtaagaaagt
     1441 agttgtgaca gaagtggtca gcttcgagaa ataagaagga atttacgaaa attgcttctt
     1501 aaatttttgt gaaatttcgg cttatttctt ggagataaaa gcttctgtcc cagcctcttt
     1561 tctatttccc cggaaacaac cgctgataat aggcatctgc ttttttatag gaaaaaatcc
     1621 aaataccaga tacaaaaaaa cgtccgaata acccgatcaa cggtacaaaa aagacaccgg
     1681 caatcaagca taagttaatc atcatggaga taagacttcg acggtccttt gacaaaactc
     1741 tcacgacatg ggaatctggt ttatttgctt tcatgatttg cctcatcaag ataccaaaag
     1801 aaaaactcca catcgtgtat actaagacat aagccatctc aggccaaaaa gcttcagggt
     1861 tacgcccgac ccaactagta acagaaggac ataaagtcag ccaaaagagc aaaaagatat
     1921 tcgaccagac cactcggtaa ttaattcgtt ctgccatctg aaatatacga tgatggttca
     1981 cccagatcac cgcaacaaat ataaagctga tggtataagc taataatgta tttcggatga
     2041 cccataaaga atgaaagcta gatgtattag ggatcttgat ctctaataca agaactctca
     2101 aaacaatggc taccactcca tcagtaaatg cttctaatct cgactttgtc acgatgattc
     2161 ctcctcagat gattcagcgc ttttatttta tcacttacgt tattaaataa ccatcgtttt
     2221 atatagggaa ttttctgaaa attgttgcat atttttcgaa attttgttac ttttaaaaaa
     2281 accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct ttttataggc
     2341 tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt tcatttgggt
     2401 caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac cggtatcggt
     2461 cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt atatgaatta
     2521 gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta cttagttatc
     2581 ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg tttagcacca
     2641 tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt attttttgtg
     2701 ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg aaagttcctg
     2761 aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt gaatccacta
     2821 ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt tttcacaggg
     2881 tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg gatcatcatt
     2941 gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa agacacgatc
     3001 aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct cgcttattta
     3061 ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac gctggctcag
     3121 attgctgatc attatttagg tacttatggc agtatcttat tagcatttat cgttatatta
     3181 gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt cacagatttg
     3241 tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc ttgtttagcg
     3301 gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat gttcatctat
     3361 ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa gcagcgtacg
     3421 gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga tgggttgaac
     3481 gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata tgttgcggaa
     3541 agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt tatcggtttt
     3601 gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg ataaaagagg
     3661 ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat ggcttctcac
     3721 atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg tttattgaga
     3781 ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa atggcttctc
     3841 acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac cgtttattga
     3901 gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct ccatatactt
     3961 tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt tcttttataa
     4021 tatagatttt agacatatat atgatataaa aatagttttc gcaaaa
//
LOCUS       DAZBTF010000140         4049 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000140 DAZBTF010000000
VERSION     DAZBTF010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.147"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(158..1663)
                     /locus_tag="ACHGNS_002862"
     CDS             complement(158..1663)
                     /locus_tag="ACHGNS_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN6938722.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDPFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1660..2346)
                     /locus_tag="ACHGNS_002863"
     CDS             complement(1660..2346)
                     /locus_tag="ACHGNS_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN6938723.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2539..3960)
                     /gene="gndA"
                     /locus_tag="ACHGNS_002864"
     CDS             complement(2539..3960)
                     /gene="gndA"
                     /locus_tag="ACHGNS_002864"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFN6938724.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1114 a          857 c          693 g         1385 t
ORIGIN      
        1 tcgttcatct aaaagatact tacatttaat taacggatgt tattctattt aattgttcgc
       61 tagttttttt ataatgacat ccctgaattt ctcacttgct tgtattttgt accttattct
      121 ttgttggata tataaaaaaa caagtaaagc acatgcttta cttgtcttct gcctttgcga
      181 taggaataaa gattctaaaa attgtccctt gccctactac actttctgct aagatacgtc
      241 ctttatagtt ttcaaccagt tgtttagcga tagacaaccc cagaccattt ccacccttcg
      301 ttctagctct tgctttatcc actcgataaa aacgattgaa gatcttatta aggtcttctt
      361 tgggtattcc ttcgccaaag tcctgaatcg cgatctcaaa ttcattcaga gtggaagaaa
      421 tagagatatg gacttctttt cgtgtagttg aatactttac tgcgttatcc aatatgataa
      481 tgattaattg ttcaaagtga ttacgataaa tttgcagggt cacttctttt gtcaaatcat
      541 catctaaagt gatcacatag tctggatata atatcttgaa attattataa acttgatagg
      601 tgacttcttt tcctttagtt gtttcattcg cataatgaac atctacttgt tctgctcttg
      661 ataagtctag catctcttga actaagcttt tcatacggct gatttcttgc atgctggcgc
      721 ttaatgattc ttctaatatc tcaggatcat ctttccccca acgatttagc agactaagat
      781 gcccttcaat aattgcaaca ggtgtcctta actcatgaga tacgtcttcc acgaattgct
      841 cttgttgttc aatatacaaa cgcatacggt caagcatctc attgaatatt tcagctaaat
      901 ctgccaattc atcatttgtg tcgatttcag gcatgtggat atctgattgc ggatctttac
      961 gtatagtatc cattgtatct ctgagtacct tgagtggttt taagaaataa gaagataaaa
     1021 agaaacctaa aatactactt aagatcaaag aaacaacttc taaaacaata agagtcaata
     1081 atagcttatt tcgaatatca tagaaagaag ataattcata gaagctttgc gcatagccga
     1141 tcttttctct tgtaccttta gaatatatag gctggataga taaaaagcca gtttttcctt
     1201 ctagtgtaac gatcgttgga actttacgtg tagtctgaat caggtttcga ctgttttcat
     1261 gggttttaaa aatcaagttc tcgtccaaat cataaacata aagatccatt gatggctgcc
     1321 ctagctcagc tataaaggga tcgatcttca ttaagtttcc ttcgaccagt gaagtatgat
     1381 ctacgacctc atctccgtca tttgtcgctt tcgtcagatt acgatacgta tttactaatg
     1441 tcagttcttc atccgagtta gccaaacgag aagctacttc tgaaatcgta cgctctacat
     1501 tgtttctttc ttttgccaca atcagattga tagatgattt ataggtaatg acagcgaaaa
     1561 ttgtaaacac aacgaatatg aagaaagaac tcgcaaaggc ccacttgatt gtcaaggagg
     1621 gccctttcag ttctcttttt tccgattttt tcggctttat cacgagcgca tcacataacc
     1681 tgttccgcgt acagtttgga tatagctttc ctccccggga acgtcaattt tgttgcgaag
     1741 ataacgaata tatacgtcca ctacgtttgt ttcaacttct gtttcgtatc cccacacttt
     1801 atttagtaaa acgtcacgtg ctaataccac gttcacgttt tccattaaag taagcagcaa
     1861 ctcgtattct ctttttgtta gttcaattac ttcatttccg cgacgtacga cacggttttc
     1921 tttttcgata gtcaaatcgc gataagtaat agtagtttgt ttagccacat ttttatcgcc
     1981 ttcaatatcg attcgacgca acaatgcacg aagacgtgct aataattctt cgatagcaaa
     2041 tggtttgacg atgtagtcat ctgctccgtg gtcaagtcca gatacacgat cgatcacaga
     2101 atctcttgca gtcatcataa tgattggtgt atttttgact tggcgtacac gtctgcagac
     2161 ttctaaaccg ttcaattctg ggagcatcag atccaacaat atggcatccc actcattatt
     2221 taatgccgca tctaaaccag ttcggccgtt gtagtggact tctgcgttat acccttcatg
     2281 cttcaactct agctcgacaa atcgtgccaa gttcttttca tcttcaatga ttagaatatt
     2341 gctcatttat tattttatcc tttctattct actggctttt taaaaactct atcattatac
     2401 cgataatatg cttaaaaatc tatagtcatt ttatcatgga tagcaatttt aaaaaagagc
     2461 tgaagtctac tcataacaat cgacttcagc tctttcgtcc tctttttatt ttctcatttc
     2521 ctaacagccg aaagactatt attcttcatg gtaccatgag taatggaaga ttccttcttt
     2581 gtcgacacgc tcataggtat gtgcgccaaa gtaatcgcgt tgggcttgga ttatatttgc
     2641 aggcagacgt tctgctctgt atgaatcgaa gtaagcgatg gctgatgaga aagtcggtac
     2701 aggtacgcca gcttggacag ccaaggcaac gacgtctctg actgattgct gatatttttt
     2761 cgtgatttca acgaagtaat catctagcaa taaattttcg atctcaggat ttttgtcata
     2821 ggcatccgta attttttgca agaatcgagc tcggatgata catccagcgc gccagatttt
     2881 tgcgatttca ccaaatggca agtcccaatc aaattctttt gaagcgacac gtaattgagc
     2941 aaatccttgg gcataactca tgattttact gaaatacaac gcttctcgga ttttttcgac
     3001 caattctttt ttgtctcccg tgaactcaaa atcgttcgta cgagacaaaa tcttgcttgc
     3061 ttgtacgcgt tcttctttat aagcagaaat atagcgggca aatacagatt cagtgatcaa
     3121 tggaagtggc acacctaagt caagcgcgct ttggcttgtc catttacctg ttcctttatt
     3181 cccagctgca tcaaggatca catctactac tggtttacct gttccttcat cgtctttacg
     3241 tgtcagaatg tcagcagtga tttctatcaa atagctgtct aattcgcctt gattccattc
     3301 tttaaagata tcagccattt cttctactga aaggtttaaa atattcttca ttaagtcata
     3361 agattcagca atcagttgca tatctccata ctcaataccg ttgtgtacca ttttcacata
     3421 atgtccggct ccatttgggc caatatatgt gacacatggt gctccgtctt ctgcttttgc
     3481 agaaatttgc tctaagattg gtgcaactag cgcataggct tctttttgtc caccaggcat
     3541 gattgaaggt ccttttaaag ctccttcttc accaccggaa acgcctgtac cgataaagtt
     3601 gatgccagag ttggctaact cttcattacg acgcatcgta tctttaaaga atgtatttcc
     3661 tccgtcaatc aaaatatcgc ctttatcaag gtgaggaagt aactcttgga ttgttgcatc
     3721 tgttgccggt cctgctttga ccattagcaa gatccgacgt ggtttctcga tagattctac
     3781 aaattcttcg attgtatatg ttgctttgaa atttttatct gggtgttctt ctactacagc
     3841 ggttgtttta gcccctgtac gattgaataa agccactgaa tagccgcggc tttctatgtt
     3901 caaggctaga tttttaccca taacggccat accgacaacg ccgatttgtt gttttgacat
     3961 atgaactcct cctacatacg ggatttctcc ctaatctctt acattatagc ttaacacggc
     4021 tacattgcaa actaaagaac aacatattt
//
LOCUS       DAZBTF010000141         3953 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000141 DAZBTF010000000
VERSION     DAZBTF010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.148"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            118..555
                     /locus_tag="ACHGNS_002865"
     CDS             118..555
                     /locus_tag="ACHGNS_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFN6938725.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            631..1251
                     /gene="rpoE"
                     /locus_tag="ACHGNS_002866"
     CDS             631..1251
                     /gene="rpoE"
                     /locus_tag="ACHGNS_002866"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFN6938726.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            1440..1946
                     /locus_tag="ACHGNS_002867"
     CDS             1440..1946
                     /locus_tag="ACHGNS_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFN6938727.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            1963..2862
                     /locus_tag="ACHGNS_002868"
     CDS             1963..2862
                     /locus_tag="ACHGNS_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFN6938728.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            2949..3833
                     /locus_tag="ACHGNS_002869"
     CDS             2949..3833
                     /locus_tag="ACHGNS_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFN6938729.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
BASE COUNT         1423 a          685 c          832 g         1013 t
ORIGIN      
        1 ctctaaaatc aaagagttgt tttggaaaaa aggttttctg taaaaatgga caagttggtt
       61 gacaaaacaa aagaaactag gaacaatgta gctatgagaa gtaaaggaga atgaataatg
      121 gaattatcac aaggaacacc gatttcaatc aaattgcgta caaaagtaaa acagcaaggg
      181 gaagtgcaag atttttattt tgatttgaaa gggcaaatgg tgacaattgg cgatacgctg
      241 tacatacgtt ataaagagat tcaggaggag acgggcgatg agattcctgt aacgatcaaa
      301 ttagttccag atggtcatgt acagctgatc cgtgcaggag aaatgcggat gcgtttgaaa
      361 tttggctata aagaacggtt agaaacatcc tatcgaacac catacggtat gatccagatt
      421 gctactttta cgaaagaact gcatgttagt ttgaaagacc gtcccacagc agggaaggtt
      481 cgaatcgatt atgatctgtt catgggaccg gaaaaaatcg gcgaatatta tttgaccttg
      541 gattttacag cgtaaatata aaagaaaagt ttgatttttt gtgtctgatt ttgtatgata
      601 aggagtagac tgtgaaagga cgtgtcctga ttggaaatta gtgtatttga aggtgcaaac
      661 aaaagcgaat tatcaatgat cgaagtagca cacgcgatct tagaacaacg tggcgatgtc
      721 atggattttt ctgatttagc taaccagatc caaaactatc ttgggaaatc agacagtgaa
      781 attcgtgatt cactcgcaca attttatact gacttgaata ttgacggcag ctttatctca
      841 ttaggggata atcgttgggg cttgcgttca tggtatgcta tcgattcgat cgatgaagaa
      901 gtaaaccatg gaatggacga agaggatgaa gatactccac gtcgccgtaa acgcaagaaa
      961 gtcaacgcct ttatcaatga tgatgaagac gcgatcgatt acaatgacga tgacccagaa
     1021 gatacagatc tgacagaaga agatgacgat gatctgtttg atgacgatga tgatgaagat
     1081 gaagaaatcg ctgcttataa ttctgacttg caagaaatcg gagcagatga cccgacagat
     1141 gatgaagaag acttgccagg aaatatcgaa gaagatttaa gtatcataga cgaagacgat
     1201 gatgatgaag attatgaaga ggaagaattt tctgactctg aaaaagaata aacattattt
     1261 tcttacgaga ggttgtgaca gagacgatca gcctatcaga ataaggaggt gtgacacgat
     1321 ttttgtcaca ccttcttttt tgtatataaa aatcccaagt aaatacataa aaaataaaaa
     1381 aattgaaact tttagcgcac cttgtacgtc atattaagtg aggcagaaag gagtggagaa
     1441 tgatttttac tggatataag aaaaaacggc agatcgaaaa ataccgtcac ctgctgaatg
     1501 gactcatcga aactgattat caaaaaatgt tccgtatcac gatgaattac gtacatgata
     1561 aagaagaggc cttagatgtg atgcaagata gttttcataa agccctgaac tattttgaaa
     1621 aagaaaaaga gatcgagcat tttagtgcat ggttttatcg gatattgatc cgcacggcat
     1681 tagatagttg gcgcagacaa aaaaggacgc ctgtggactt atttgaaatt caagatatcc
     1741 cccaacagtt acctttagaa acttccgttg cagagctcca tatgatttta gataaaatag
     1801 acagtccgga aaaagagatt ctgatcctct acttttttga aggatttcgt ttgaaagaaa
     1861 ttgcgactat cttggatatg aatgaaaaca cagttaaaac gaaaatgtac cgctcattga
     1921 atgctttacg tcaaatccta gaatgaagga gagaagagtg ccatgtattc ccaaaaagaa
     1981 aatttaaaaa aactaaagaa aacttataaa gaacaagcaa ttcccaaagg gcttaaagag
     2041 gagatccgcc agcgttttat tatcgaagag caatcattta taaaagcaaa gcggagaaag
     2101 agacgtctcc aagggttcgg agtatctttg gctatcgtaa tgattacaac aagtagcctt
     2161 ctttttaata gtcaagttcg aagctttgca gaagatttac ccatcttagg atcagtcatt
     2221 gaattgatct taggcgaacg ctttactgat cgatctgaaa aaatcgatat tcaggtaccg
     2281 aagatcaata cacaaaatga gaaagaaaat aaaacaattc atggattaaa tcaaaaatac
     2341 tttcgcgaag gacaagcaga ttttgaaaaa gctgaaaaag aatatggaaa ttttgaaacg
     2401 gatcattacc aagttttggg agattatcag aaaatcgtag acgataaccg gtttctcgtg
     2461 attgaaaggc agatcaccca aacagcagca gatagccatg ttgaaaaaag atacgacaca
     2521 atcgacaaga aaaacagtgt ccagctctca cttccacttt tattcaaaga tgacacctat
     2581 ctatctgttt tgacaaaaga agtcaaacgg cagatggccg aacaagtcaa ggaagatcca
     2641 tctaagtact attggactga gcaagatatt caagaaggga cgatagaaaa accaacactt
     2701 gttacgccta cacgaagttt ttatttgaac aaagagcatc aattagtttt gactttttct
     2761 caatacgaaa ttgctcctgg ttatatgggt acaccagaat ttgtcattcc taagtccgtg
     2821 acaaaaacga ttttggcatc ggaagattat ttggatcatt agacgagaag tagttgcgac
     2881 tagcagtttc aattttcaag taagtacaag taaataataa gaagagaaaa agagaaagaa
     2941 gagatatagt gaaaagaaat tatttagtta tcagtattat tgctttatcc gtgcttttgt
     3001 ttttcgaaat tacttcggtg gcctccttga tcaccgaagt aaaaggacaa acacactctt
     3061 cggagcattc gtcacaatct ttcaaacaat cagaacaatc aaccattaaa agccaatcaa
     3121 ctgaaacagc taacgtcaaa gaagccattc aagaatcagc ggaaaaggta caaagtcagt
     3181 catcacaatc aacacaagac agtacaaaaa cgagccgaga tccggctgtc aaaagcgtcg
     3241 caatcagctt tgatgatgga ccaggagcga ccacaacacc gcagcttctg cgaatcttaa
     3301 aagaaaaaaa cgtgcatgcc acttttttcg tcttaggaga aaatacggcg cagcatcctg
     3361 aaatcgtgaa gcagacagct gaagcagggc acgagattgg caatcacaca tacgatcatc
     3421 agaatctggc cactctttct gcccagtcga tgacggagga agtgacgaaa gcagacactg
     3481 agatcaaaaa agtcactggc aaaaccccca cttttgttcg tccgccctat ggttctgtca
     3541 cgaatgtagg agcaacgatc atccagcgac cgatcatcga atggtctgtt gattcagaag
     3601 attggaagac aagaaatcct gatttgattt tgcagaagat ccaagcaaca gtatacgatg
     3661 gggcaattat tttattccat gatatctatc ctgaaacgat tcgtgccgtt cctcaagtga
     3721 tcgattatct gaaagagcaa gggtatcgca tcacgacagt tggggattta ttagggcatc
     3781 caacggctgt cgagaactat tatggcagaa acgatcatcg gccagttcag taagtgagac
     3841 aagtatcttt aataaataac acattttaat aagtagttga acacataact accttacttg
     3901 caaaggctta acaatatcta tacaggtaca acaaaaaact gtccaccaat ttt
//
LOCUS       DAZBTF010000142         3921 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000142 DAZBTF010000000
VERSION     DAZBTF010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.149"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..453
                     /locus_tag="ACHGNS_002870"
     CDS             <1..453
                     /locus_tag="ACHGNS_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFN6938730.1"
                     /translation="SQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            426..1103
                     /locus_tag="ACHGNS_002871"
     CDS             426..1103
                     /locus_tag="ACHGNS_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFN6938731.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            1054..2235
                     /locus_tag="ACHGNS_002872"
     CDS             1054..2235
                     /locus_tag="ACHGNS_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFN6938732.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            2248..3225
                     /locus_tag="ACHGNS_002873"
     CDS             2248..3225
                     /locus_tag="ACHGNS_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN6938733.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            3225..>3921
                     /locus_tag="ACHGNS_002874"
     CDS             3225..>3921
                     /locus_tag="ACHGNS_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN6938734.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKF"
BASE COUNT         1468 a          481 c          732 g         1240 t
ORIGIN      
        1 tctcaaccaa ttatttgcat tttacctaat agtgatgctg gtagcgaaat tatcagaaaa
       61 atgtaccaga aatatttaaa aacggatatt atttatatta cgaatttaga gccggaggaa
      121 tacctgacta tattaaaaga gtgcaaattt atgatcggaa attcttcttc tgggataatt
      181 gaagctgcat cattcaaaat accagtaata aatttaggaa ccagacaaaa tggacgagaa
      241 caatctaaaa atattataaa tgcaaatcta aatgaaaatg aaattgaaac tgctttgaat
      301 aaaatacaag atcctcagtt tataaaaagc ttgaaaaatg ttaagaacat ctacggtgac
      361 ggcaacagtt ctagaagaat aacaaaaaca ttgaatcaaa tattgaatga tggggaagat
      421 attaagtgga ttcaaaaaca aatagcttat taaaatatac tagtgatcca tacattatta
      481 ttggggctgg cggacacgca aaagtcatta ttgaaacctt aagattagaa aataagttaa
      541 tatatggatt ttttgatgat aagcaaaata aaatgcaaga tctgaaatat cttgggaaaa
      601 taaacgaatt gaatagctgg gcggatagca aaaaaaaaat ctataaattt attgtagcta
      661 ttggtgactg tgtagtaaga aaaaagatta taaaaaaatt gaatatgcca agtgagcaat
      721 atggaatagc tgttcatcca agtgctatag tggcaaaaga aatttcaatt ggttatggaa
      781 ctgttatttt tgcgaatgct gtaattaacc cagatgctgt aattggagag catgctataa
      841 taaacacagg tagcattgtt gagcatgatt gtagaattaa taactatgtt catttatctc
      901 caggtgtatg cttagcgggt ggagtacatg taggtgtagg aactcaaata ggaattggtt
      961 ctcaatgtat ccagatgaag aaaattggat cttggtgtat gataggcgca gggagcacta
     1021 ttgttaagga tattccctca cattccttag cttatggcaa tccagctaaa attaagaaag
     1081 aaggaattaa ttttgaattc tagaatttta ttatcaagtc ctcatatggg aggaacagaa
     1141 gaaaaatatg ttgctaaggc ttttaaagat aattggatag cgccattagg tgaaaatgtg
     1201 gataaatttg aagaaagtat tacaaaatat actggttcta agggcggagc ttctgcgtta
     1261 agttcaggta cagctgccat acatcttgcc ttaagactgc ttaatgttgg aaaaaacgat
     1321 attgtctttg ctccttcatt tacttttata gctacagtaa atcctatttt atatcaaggg
     1381 gctgtaccag tgttcattga ctcagaacct aatacatgga atatgagccc tgttgcacta
     1441 gagagcgcat tagaagaagc tgaaagaaac aaccaattac ctaaggcaat catcgtagtg
     1501 aatttatatg gtcaaatggc tgattatgaa cgaattttag ctattgcaaa ccaatttaat
     1561 gttccagtga tagaagatgc agcggaatcg ttaggttcta cttataaggg aatttcgtca
     1621 ggcttatttg ggacaattgg gatctattca tttaacggta ataagatcat cacaacctca
     1681 ggtggaggag ctctagtgtc tagtgataaa aaatatgttg agcattcaag atttttagca
     1741 acacaagcaa gagatcaagc accgtattat caacatagcg aaataggtta taattaccgt
     1801 ctaagcaata tttctgccgg tatcggacgt ggacaaatgg aagtattgaa tgatagagta
     1861 gctaaacgaa gagaaatttt caatcgatat aaggaagctt tttctggaat tgatagaatt
     1921 gaaatgatgc cagaagtgga tggcatggtg ccaaatcgtt ggttgtctac tttaacgatc
     1981 aatgattata attctattga tcaagtagtt aacgtaataa ataaactaaa tgaaaaaaac
     2041 attgaaacaa gagctttgtg gaaaccacta catttgcaac cagtatttca aggggtaaaa
     2101 atgtattacc atgataataa tcttagtgtg tcagaaaagt tatttaatag cggcttatgc
     2161 ttaccttctg gaagtaatat gacagatagt gaacaagaat ttgttattga tgagtttaaa
     2221 aaaatattca agtaggtgtg atattttatg aacataggaa ttattggttt aggttccatg
     2281 gggaaaagaa gacttagact cattcaagaa cactttaatg atattttact atgtggtgtt
     2341 gatactaatg aggcgagacg aaaggaagtt gaaaaggaat ttggaatacc aacttataat
     2401 agtctaacag aggcaaaaaa tttgtttgaa ataaattcag tttttgtttg tacatcccct
     2461 ataacccatg aatttattat aaaagaggct ttaaatttag attgtaatat ctttacggaa
     2521 atcaatttat taaatgaata ttatgatgaa ataatcgaag tagctaaaaa gaaagataga
     2581 ttactttatc tatcttctac atttttgaag agaaaagaaa ttcaatatat taagtctaaa
     2641 gtgacgcata ataaaaaaat ttcttatcaa taccatgtag gacagtattt acccgactgg
     2701 catccttggg aagattatcg caattttttt gtttcaaaca aaaaaactaa tgggtgtaga
     2761 gaaatattcg ctattgaatt accttggatt atagatacat ttgggaaagt taaatcgttt
     2821 actgtaatga cccagaaatt atccaatctt gatattgatt attcggacag tttcactctt
     2881 attattgagc atgaaactgg tatagttgga actttaagtg tagatgttgt ttcaagagta
     2941 gctaaaagaa atttaactat aattggtgaa aagattttaa tagaatggga aggtaacccg
     3001 gattctctat tttcatggaa tgaagaaact tctagtatga cagatattca attgtacgat
     3061 gaggttatgc atgataataa ttattcacat actattattg aagatgcata tttagaggag
     3121 attaaagaat ttttcacgct tttggaaaag aaaatggaac cccaatatac ttttgaaaaa
     3181 gataagtata ttattaactt aattaacgac attgaggtaa attaatgatg aaagtaattt
     3241 tttttgggtt agggtcaatt ggaaagagac atctaaaaaa tctaataagc tatggaaaaa
     3301 agaattctat taattttgat ataacagctt atagtagcag tggaaaaaaa gagtttgaag
     3361 aaataacgta tattcaaaat agagacgatt tggatacaca ttatgatata gcttttatta
     3421 caaatccgac ttttaagcat ctggaaacac taaatgtaat tgttgaaaaa gttgattaca
     3481 tatttttaga aaaacctgct ttttctgaga gtattgatct tactcaatat aattatgatt
     3541 acaaaaatat ttatgttgct gctccactga ggtataaaaa tatcatggaa gttgttcgta
     3601 atagtattca aagtaaaaaa gtatattctg taagagctat ttgttctact tatcttcctg
     3661 attggagaaa aggtactgat tatagaaata attattctgc ctttaaatct atggggggag
     3721 gagtagaatt agactgtata catgaattgg attatattat aaacctattt ggtttcccta
     3781 aatcagtaaa aaaacagttt ggtaaatatt cgaatcttga aattaacagt aatgatttag
     3841 caacatatat attgagatac gatgataaaa ttatagaaat tcatttagat tattttggaa
     3901 gagttcctac aagaaaattt c
//
LOCUS       DAZBTF010000143         3893 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000143 DAZBTF010000000
VERSION     DAZBTF010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.150"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(74..616)
                     /locus_tag="ACHGNS_002875"
     CDS             complement(74..616)
                     /locus_tag="ACHGNS_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFN6938735.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            751..1140
                     /locus_tag="ACHGNS_002876"
     CDS             751..1140
                     /locus_tag="ACHGNS_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938736.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1144..1908
                     /locus_tag="ACHGNS_002877"
     CDS             1144..1908
                     /locus_tag="ACHGNS_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFN6938737.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIQLMEEASTFLAKNLIKKYK"
     gene            2037..2984
                     /locus_tag="ACHGNS_002878"
     CDS             2037..2984
                     /locus_tag="ACHGNS_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN6938738.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3114..3854
                     /locus_tag="ACHGNS_002879"
     CDS             3114..3854
                     /locus_tag="ACHGNS_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938739.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1298 a          661 c          794 g         1140 t
ORIGIN      
        1 tcaaaataat aaataaaaac tgtctaacat ttaaaattta atttagatat tagacagttt
       61 ttaagtttgt gagttatttt ccttgtcgtt ttctcacttt tttagtccag aaaccacctg
      121 aaatatattt tggctcataa gaaatgataa aagcacgctc ttcgatttct ttgatcaggc
      181 gatacagttc acgttcgctt tttctaggag aaagtatttc gagaactagc cgctcacctt
      241 ccagccccat tgcagaagtc tgtgtcacac cataaccgtg ttctctcagt atacccggca
      301 aacttttatc ttctgcgcta tttgctggca aaatcgttgt gaccatgatg tatcctaatg
      361 ccaatttgtc ttcaatcaaa attccgacac tgatacctac tgcgtaacct aacgcataga
      421 caaataaatt cagtggatta tctaaacgat ctaaaacaag ggataagccg acgatataaa
      481 tcgtgatctc gaccatactg acaagtggag caatcagtcg atagcctttc attgtcaaca
      541 tgaaacgtaa cgtgttcaga gtgatataag caaaattaat gaggaagatc atccccaaca
      601 tttttggatc aatcatttgt aaaactcctt tcatttatat aacaacaata tcaaaaaaaa
      661 tcgtaagtga agaaaaattg aaactttata catacaaaaa aatgataaaa tgattataat
      721 agaattataa agataggtta ggtgattaaa atgaaaacgg tcaatcgtgt gttttgtgcc
      781 agccacgtgc taagtctcct tctcagtata tacttaggtg tctattcgat tcaacagcca
      841 attgaggtag agaacattgt ttttcagatg tcgctgacag atggtttaat tttaagcgtt
      901 tttttctttt tgatactgtt tattggtaat attttcggta gcgttgccag ttttttgaat
      961 ttttctattt atccactcct ttcgctagca ttaggagtgg tgggactttg ttctctgtgc
     1021 ttatttcccg agccattcag tcctgcattt atattatttg gtacactgaa cctgttccag
     1081 acaactgttg gagcatggtt gctatggcga tcaggaaatt taatgaaaat aggtgagtaa
     1141 caattgaatc caattatatt gaaacgttct gtccatcata ttccagtgtt agaagttgtt
     1201 ctgaaagaat taagagagga acgtattccc gttgtcatct attatcacgg atggcagaca
     1261 agtaaggagt tagttttgac gcaaggaaga aaaattgcgc agcagggcat tcgtgtgatc
     1321 ctgccagatg cgatgaatca tggggagcgc aaacagccgg tttcttctat tccatcctat
     1381 acattttggg atagtatcta cggaaatctg tttgaatttg acacacttat caagcattta
     1441 gaaaaaagag gtcttctaca tgatcagctt gcagttggcg gggtttcgat ggggggaatg
     1501 acaacatgcg ccttgttggc taaacatcct catatagtag cgggcgtgtg tttgatgggt
     1561 tcccctgcac ctttggaata tggaaaagaa atcgtaaggc gcgcatcgga atataattat
     1621 gtcgtaccaa aagactattt tgatttgatt tcatgggtta agagctatga tttgtcattg
     1681 cagccggaaa agttagctgg acgtcctctg ttcttttggc atggacaagc tgacgaaaaa
     1741 atcccttata tacaaacgga acgatttgta gaaaaaaatc ggcataagtc ttacggaaaa
     1801 gaaattgttt ttcgttcctc aaaaacacag cgacatatgg tacaaataca actaatggaa
     1861 gaagcttcca cttttttagc aaaaaatttg ataaaaaagt ataaataatt gaaacggaaa
     1921 atcactcatg acgatagagg attttccgtt ttttattttg ggctataata aactctgtta
     1981 ggaaaatttg gtataattga tagtaagaat gacaacatgt tagaaaggaa gagagcatgc
     2041 ccactttagc agatgttgca aaacgtgcca atgtttcaaa aatgacggtc tctcgagtca
     2101 tcaatcatcc ggaacaggtg actgacgaat tgaaagagtt agttttctca gcaatggctg
     2161 aactggatta ccgtccaaat attgctgcaa aagcacttgt cacgaatcgg tcgcagatca
     2221 tcaaattatt tatactggaa gaaatcgaca caactgagcc ctattacatg aatttgctga
     2281 tgggaatcgc taaacggatc ggtaaagcgc attattctct ccaattagtt acaaatgatg
     2341 ccttcgacgt cggttcttgt gatggataca tcatcacagg tgtcagaaaa aaagacttcg
     2401 attggatcga ccgtttggaa aaaccagttg tcttgtttgg ggaaaattct gaaggatatg
     2461 attatgtaga cagcgataat gaatatggta cagcaaaagc aacgcaatat gccttagaaa
     2521 gagattatca aacgattatt tatatcggta tcgaagtgga cgagcctttt gaaatttcaa
     2581 gggaaactgg ttatttatct gtgatgaagg aagttgatct tcctagtaaa atcttgcggt
     2641 ttccaaatca ttcgacagaa gtagaaagat atatgacaga aaactgggca gattttacag
     2701 ataaaacttg ttttgtatgt agttcagatc ggctagcact aggagcagta agagggatta
     2761 taaatgctgg aggacacttg cccgaagaat ttggtgtgat tggatttgat ggggtatttt
     2821 tagaccaaat cagttcacct aaattgacaa cgatcaagca agatattata cgtatgggtg
     2881 aagtatgtgg agaaatgttg ttaaaaaaaa tagaagaaaa aggggcgagt caaggttatc
     2941 gtcgtttttt tcctgaacta gttatcagag agacaacgaa ttaagtctta ttactatata
     3001 tatatttatg aaattcatcg ctattttctt aaaataatca gcgcattgct actgaaaaca
     3061 atgaatctgt gctattattg agaaaagttc catttttgag aaggatggtt tgaatgaaag
     3121 aaatcagacg aaaacatttc cgttttcctg cgtacgatga tgagttaggc gtaaaattaa
     3181 ccaacgacaa gaagcgagta ttatttgacg gacaagaaga tcttttgact gaacagaatc
     3241 agtccccgtt tgaacagatg aaagaatttc atttcacgaa cacgaacacc gagaaaatcc
     3301 gtcgcaagcg tcgtgcaaca aatggattga agtcagctcc gcgtaaggaa aatttggaaa
     3361 agcataaagg aaaattaccg gattataccg gccattacca atcagaaatc acatcgacag
     3421 gacgaaaaaa actttttggt gattcctctc gttctgtcag acaaagtgaa acaaggacag
     3481 agtccaagca tactacacga aacgcatctg ttgacacgaa ggtgcgggag cgttcttatt
     3541 ttgttcctaa atatgtaccc gcatcgatta tcccagaccc tagagaaagc aaaatttctg
     3601 aggatcattt aatcgaatcg atgaaaaaaa cggatgactc ttatctgttg ttcgatacag
     3661 aaccgacgcc gtatcaagaa aaaagaaatg gtgagccgtc tgtacgaaca ttcaatcgaa
     3721 caaaaacgtt agaggaaaca aagcagtcag aagaaaaaaa atcgaaaaaa agccatggta
     3781 tcttagaacg ctcattacaa ggattgatcg atgatcaagg aaactctttg ggagaaaata
     3841 gctattttaa ataagtgata tacaaattta tattcggcta cagttggaaa gga
//
LOCUS       DAZBTF010000144         3878 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000144 DAZBTF010000000
VERSION     DAZBTF010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.151"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(718..3867)
                     /locus_tag="ACHGNS_002880"
     CDS             complement(718..3867)
                     /locus_tag="ACHGNS_002880"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFN6938740.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         1076 a          803 c          637 g         1362 t
ORIGIN      
        1 ggactaatac aggtagtcct cttttttgtt attttgatat tctaaatcat tttttattgg
       61 attattccct tgttcgatat agacccaaat tgatctatca ataattatca aactgagtca
      121 tatagagaca aaaatttcaa aaatgacctg ttaaatggtc atcgaatatc atttaaaatc
      181 aatacgacgg agtcagacac ggagtcaaaa caagcccgct gagagtaaaa aatatataca
      241 gtcccatagg taaagtgcca caggactgta tattttttta tttgagatta cctgaaatga
      301 ttccatttac tttttatctc attttttcct actagttagt atcaaatact ctcctatttt
      361 ctcaataaac tattttgggt tacatggtat ctgaaacagc taaaaccttg tcattacagc
      421 aaaaaatcac tttatctccg tttcatttca gatacatttc acttaagcgg attccaaaaa
      481 aacaaaagaa aaaggaaccc ctagttagcc tatgcttttt attggttcct ctcaaaacac
      541 ggtcatataa caacggggtg gggtaaatga atgaaaaagg tggaggaaac attattcaat
      601 agctgtctta aatacttcgt tataaaatgt gaacaaataa aagacatcta tccctatttt
      661 ctacacttcc actttactac catctttgaa tcgtacaatt ttcttaactc ttctcgttta
      721 cctcaattca ttttcaagtg gaatcagctc atcatctaac attttacgcc atgctcgctt
      781 catttcttgt gggtatttaa atggcttcgc ttctggatgg ttggctttat atccttcgta
      841 atccttactg ttgataattg ctttcatatt aggaattgga tcttcacctg gcatatattg
      901 cagattagaa gcatgtaatt ctttctcaga aacaaaccac tcattggaaa agacatgaat
      961 caaatcatca ctcgcttgtg taaaatagcg acgtttaatg acaaagatat cttcattact
     1021 atctaaagtc cccacatcaa acgcatttaa aatttgtcgg taaacttctt tgactccttc
     1081 tgttttattc aatttagcca aagctttttc aatttcttct cgtgcatctg aactattcgc
     1141 cgcataagtt cctagtaatt gatcaatata agtagaatca atatcataca acttcgtggt
     1201 attttcacta taaaattcga tcgtggaaaa atcttctccc tctttttctg gttcttcctc
     1261 tattaatgaa cccttcacgg tctcgtacac gccaatttgt tcttccaaaa gttgaatttg
     1321 attgttcaag gttgaagatt tcaccatgtc atcattgtaa tcgtcataag tcactaacga
     1381 ttcataagca cgattcaatt cttggaatgc tttgacgtac tcgatacgga tttcaagtgg
     1441 cgtatgttca tcaatggatt ccatggatga aacctctttt aacttaccat atgcttgctt
     1501 gaatcttttt ttggattcct gataacttgg ataaatcaaa cccgattctt cttttgcttc
     1561 tgaatacagt tttgtcgcat cctcgacatt cttctccatt gtggctggct ttcggaaagt
     1621 aacaattagt ccttttgttt tttctggata cgtacggttc gtacgtgaaa aagcttggat
     1681 caatcctgca tactctagat tacgatccac aaatagggtt tgaatcgtag gtgcgtcaaa
     1741 acctgtcaat agacgatcca ctacgatcac taaatcaatt tgtttcccaa attgtttgaa
     1801 ttccgctctt tttcgtgcca aacgattatt gatgtctccg ttataacgct caatgtctgc
     1861 taatgaccag gaagtatcat aatactgatt atattcctca ataatttttt gcatttcttc
     1921 ttgttggaca cttgaatctc ggctgttttc ttctaatgaa taggtaatag cgattcgtgg
     1981 aaaatcagga tcctccatcg ttcttccttc acgaatgggc tgattaggaa actcttttgt
     2041 taaccagtca ggttctttcg tcatttcttt gatcgctcga taatactttt tggccatgtc
     2101 aatggaactc gtggttaaga tcgcagactt cgttggacgg ccatttcgaa aatcaaattt
     2161 tgtataggca ttgtctggac ggaacatttt atgaatgact ttttggatat gttcatctgc
     2221 ttcaaaaaca gtagggtcca aataactttc ttttttgaca ggctccattt gatcaatcat
     2281 acgattgatc tgttccggtg aatactctgc atatgtttct acttctttta atttccgata
     2341 gattttattc tctaatgaag tcggctccac ggtgttttca tgctctactt gaaatcctaa
     2401 aaccgagcca tcttctaatg cattcttgat ggtataggta tgcaagacct ctccatactg
     2461 atcatgggtc gtacgtgcca attgcccttt ggcttgtttc cgattctcag ggaaaatcgg
     2521 tgtacctgta aagccaaacc aagtggattt cggaaacatt tttttgattt cttccatatt
     2581 ttcagcactt aacgctcgat gacactcatc tactacaaac acgatatgtt gccccattaa
     2641 tttttggaag cgattggttc cttttttctc ttcttgtttt tttgcatatt tcaacgccgc
     2701 atctagcttt tgacgggttg taataatcac aacatttgca ttggcatctg ccaacagagt
     2761 ttcacttaac tctttggcac ttcctgttcc cacgatcaag ctattcgctt tggcgttccc
     2821 ggaagaaatc ccagtattaa attccgaagc aaatttcgta aactctgtgg tggtttgatt
     2881 atctaaatct ttgcggtcca ctaacatgat cgtccgatcg atccccgatt ttcgtgctaa
     2941 aagcttagta gatacaaaac tagtcaacgt ttttcctgaa ccagtcgcat gccacacata
     3001 tcctgattga tgtttatttg cagcaataaa caaggcttga atcgcatgta cttgataagg
     3061 atgtaacacc atgaggattt tattgtcttg atcttcactg acaatcgtat aatccgcaat
     3121 caagcgatga gcatccggga tatttagcac ttgtttgcaa aactcatata agttttctac
     3181 ctttttatta tctttcgttc gccaactaaa taaaaactta ggatgcaaat cctttggtaa
     3241 ggcatttgca aaataccgag tggtttgttc attggaaacc acaaacaact gtaaggtcga
     3301 aaagatattg ttccgaaaca taccttcttc tgcatatttt ttgatttggt taaacgcctg
     3361 ataaaaacta tcttttgcgg ttacttgttt cagctcgatt tggacaatcg gtaatccatt
     3421 gatcagtaag gtgacgtcaa aacgtcgatc ccgtgcttct atgttacttc cacgttttgc
     3481 aatttgatgg accacttcat aggtagagat tcctccacca atgtcttgat tagaataaag
     3541 aatgagcgag acagaaccca gttgcggatc ttcccgttca atcgtgatcc gagccattcc
     3601 attttcacct tttaaccatt tcgcagcatc aaagggggtt ttcgttcgta gtaacaattc
     3661 tgttttgatc gtttcaaatt ctttatcggt caaaggaaaa tcattcagtt ctgcttgatt
     3721 atttgagatg attttttggc gtaaattgac ccaaagatct tcttctgatt ttagatcggg
     3781 acgatacgtc cactgattat gcccttcccc caatacacgg atcaactgtt cttcgacttc
     3841 cgcttcctca cggtgactaa ttttcattta tcttccct
//
LOCUS       DAZBTF010000145         3826 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000145 DAZBTF010000000
VERSION     DAZBTF010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.152"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(163..2160)
                     /gene="tkt"
                     /locus_tag="ACHGNS_002881"
     CDS             complement(163..2160)
                     /gene="tkt"
                     /locus_tag="ACHGNS_002881"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFN6938741.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2262..2513)
                     /locus_tag="ACHGNS_002882"
     CDS             complement(2262..2513)
                     /locus_tag="ACHGNS_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFN6938742.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2660..3283
                     /gene="lexA"
                     /locus_tag="ACHGNS_002883"
     CDS             2660..3283
                     /gene="lexA"
                     /locus_tag="ACHGNS_002883"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFN6938743.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3463..3681
                     /locus_tag="ACHGNS_002884"
     CDS             3463..3681
                     /locus_tag="ACHGNS_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938744.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1060 a          813 c          712 g         1241 t
ORIGIN      
        1 tctcaagaaa cacaaactaa tccgaagaat tctttgtttt gtcatttaaa tgtaacatag
       61 ccgaaaacga aaagtaatat tgatttattc ttctaacaca taaagaacag ctgtctccaa
      121 cttgcagaca gctgttctta tcagtcaaac aattgcttgg atctatgata gttgattgta
      181 cgtattcacg acattttcta ctgtaaatcc aaattcttct aaaattttag tgccaggggc
      241 agatgcaccg aaatgatcga ttgtaatcgt tgcgccctct gttcctacgt agcgttccca
      301 tccaaatgat gcagcagctt cgatagccac acgtttcttc actgatttag gaagtacact
      361 ttctttatat tcagaagatt gtttttcaaa gaggtcaaag cttggcatag aaacaacaga
      421 aacatctttg cctttttcag ccaattcttt ttgagctttc acagccaaat caacttcaga
      481 acctgttgca atcaaaatac cttctggtgt ttctccttgt gatggagaaa gaacatacgc
      541 gccttttttc accatttcat ctgctacttc ttttgtagaa ggcaatactg gaaggttttg
      601 acgactcaat acaaggatag taggcgcatc tgttgtctcc atcgctactt tccaagcagc
      661 acgtgtttca tttccgtctg ccggacgaat cacttgaaca ccaggcatgc agcgaacaga
      721 tgctagttgt tcaatcggtt cgtgcgttgg tccgtcttct ccgacagcaa ccgagtcatg
      781 tgttaagacg taaacaacag gagtatgttg gattgctgct aaacgaactg ctggtcttag
      841 ataatcgaca aagacaaaga atgttccacc gtagatacga gttccgccat gaagttggat
      901 accattcata gccgaagcca ttgcaaattc gcggacaccg aaccagatgt tgcgtccttc
      961 ataatgttca ggagtgaaat ctttatctgc agccaccatt gtattattgg aacctgacaa
     1021 gtccgctgaa ccaccccaga agcttggtat agctttcgat aattcttgga tgacttcttt
     1081 acttgaaaca cggcttgctt gactgcttcc aacttcataa gttggcaatt ctgcatccca
     1141 gttttctggt agtttgccat caaaagcatc ttcaaattgt tgtgctaatt caggataagc
     1201 ttttttataa ttggcaaaca tttcattcca tgcatcttca gctttttgtc cttcttctat
     1261 catcgtttga tggaagcggg cagctacttc ttctggaaca gtgaaatctg gatactccca
     1321 accataaact tctttagcca tcttgatgcc atcttcacca agaggagcac catgaacagc
     1381 agatgttcct tctttaggag caccatagcc gatgactgtt tttacttcga tcaatgttgg
     1441 tttgtctgtt tcagcttttg cttcttcaat cgctttagag attgcttcta ggtcatttcc
     1501 gtctttcact aagatatgtt gccagccata tgcttcatag cgagcaccaa cgttttctgt
     1561 gaaagctttt gaagttggac cgtctagtga aatatcgttt gaatcgtata aaacgatcaa
     1621 tttccctagt ttcatatgac ctgccatcga acttgcttct tgagataccc cttccatcaa
     1681 gtctccgtca ccgcatattg catacgtata gtgatccatc acattaaagt tttctttgtt
     1741 atacgttgct gcaaggtgcg cttctgccat tgccataccg acagccattg caatcccttg
     1801 tcctaatggc cctgtagtgg cttctacacc atctgtatga tgtacttcag gatgtcctgg
     1861 tgttttagaa tcccattgac ggaattgttt caaatcatcg attgtcactt gatagccagc
     1921 taaatgaagc aagctataca acattgctga cccatgtcct gccgaaagaa cgaaacggtc
     1981 gcggtcagcc cagttttttg atgtcgttgg atttactttc aggtgttttg tccataacgc
     2041 atatgccata ggtgctgcgc ccatcggcaa tccaggatgt cctgagtttg ctttttggac
     2101 tgcttcaata cttaatgtac gaattgtgtt aacgccaagt tggtcaattt tgtcaaacaa
     2161 aataacccct cctattaatt taggtttttt ttacctttat ttcttacact ttcattctat
     2221 aagatgtcac acgaaaaagc aatctttttc agtatttttt actaaaagtt gtcatttcgg
     2281 ttgtgtaagc cttttttctt ttggatttct tttaattttt ccggggtcac gtcattgccc
     2341 tcaggatcga cgactttcat tccttcgata tgatgacgca taccggaacg aaaagcttcc
     2401 aaatactctt tgcgcaagtt gatttgttct tgttcttcgg cttttgagag accattttcc
     2461 ttctttttac gtgcaagttc attgatccgc tctatttttt caggtgacag catgctcagc
     2521 cctcctttta ctctccttca tattatataa aaactgaaaa aaaaacaact agataaacta
     2581 aataaaatac gaacattcgt ttgatttagc gctttttata tggtactatt tatttatcaa
     2641 gtgaaagaag gtgtaccttg tggcccgaca aactgaatca agacaaatcg aagtcttacg
     2701 atatatccat gaacaagtaa cccaaaaagg atacccgccg actgtccgtg aaatcgggga
     2761 agctgttcag ctttcttcta cttctactgt tcacggtcat cttgcacggc ttgaaaaaaa
     2821 agggtttatc caaagagatc cgacgaaacc tcgtgcaatc gagttgacaa aacaaggttt
     2881 agaaaagatt gggattcgtc caacaactat tccagtactt ggcgtcgtaa cagctggaga
     2941 acccatttta gcagtagagg atgcttcaga cttctttcca atccctccta atctttcttc
     3001 cgaagaaggc agtctcttta tgctgacaat tcgtggagat agtatgatca atgctgggat
     3061 attggatggt gacaacgtga tcgttcggaa gcaagaaact gctcaaaatg gcgatattgt
     3121 cattgcaatg actgccgagg acgaagcgac ttgcaaacga ttctataaag aaaaaaactt
     3181 tttccgtctg cagcctgaaa acgacttgct cgaaccaatc atcttagatc aagtatctat
     3241 attaggacga gtagtaggac tttatcgtag tcatatctat taattgctac taattaaaag
     3301 aaaacttttt aaaaagagtt tgagacgttg gtctcaaact cttttttcgc ccgggttctt
     3361 cttctttcaa atttttatca cttccctctt tacaaaccga acaaatattc gtatactaaa
     3421 tatacgaaca attgttcgca taaattaaag aggtgagaat tcatgaaatc aatgagacgt
     3481 cttggcttag tattaactgt tttgttttta ggtattatta ttggtaattt cggcatacgt
     3541 ccagctttac ttgtgtttgt tccgttattt actttgtggt tcatgttgtg ggatgaaaag
     3601 aagtaccgtc aatctgagcg gcgcagaatt catgaagatc atttctatca tgagccctac
     3661 tacagatcca ggcaatcata ataaggattg actgctaatt attcaaaaga caagaagtta
     3721 aacttttacg ggggtgtgac aaaaatcggt cacatccttt ttttcgaata aacggtgttc
     3781 taaagccagt ttttcggtat taagtcaaat tgcggaaaaa gtgatt
//
LOCUS       DAZBTF010000146         3819 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000146 DAZBTF010000000
VERSION     DAZBTF010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.153"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..387
                     /locus_tag="ACHGNS_002885"
     CDS             <1..387
                     /locus_tag="ACHGNS_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938745.1"
                     /translation="LLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWL
                     LFTRIYIFLMSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIG
                     ILEGIIFYIGYFPNKNNNFYFYRNKL"
     gene            complement(513..1190)
                     /gene="nth"
                     /locus_tag="ACHGNS_002886"
                     /pseudo
     CDS             complement(513..1190)
                     /gene="nth"
                     /locus_tag="ACHGNS_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
     gene            complement(1197..1895)
                     /locus_tag="ACHGNS_002887"
     CDS             complement(1197..1895)
                     /locus_tag="ACHGNS_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFN6938746.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2163..3347
                     /locus_tag="ACHGNS_002888"
     CDS             2163..3347
                     /locus_tag="ACHGNS_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFN6938747.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3353..>3819)
                     /locus_tag="ACHGNS_002889"
     CDS             complement(3353..>3819)
                     /locus_tag="ACHGNS_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFN6938748.1"
                     /translation="KEKEGVEMDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNE
                     LEVIEHIGKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQL
                     TPIGQKIRNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT          984 a          757 c          630 g         1448 t
ORIGIN      
        1 ttattaaata aatttacttt aattgctcct atccttgcat ttattatttt ttctgtactt
       61 ttaagcacta ctcttaaagg aaaatttatg gaaagaagct ctcatgcaat gattctcact
      121 tttttatggt tgttgttcac cagaatctat atttttctga tgagcctgaa accgcccaaa
      181 tccattagtt tatgtttagt tataaatggt atttttttat tatcacttat catttttatt
      241 acacctttag atagatacgt cacttattta tataatcctt tggaatactg gacttatttt
      301 ataggtatat tggaaggcat tattttctac atcgggtatt ttcccaataa aaataacaac
      361 ttttattttt atcgcaataa attgtaataa gttaccatcc tagctactga gtatgtacat
      421 ttactttttc atgcaaaaaa gataaacgaa acagcttttc tccataataa caagataatt
      481 catacattta tttgcaatta aagaaaccac gtctacaggc gtggtttctt ttttagcgcc
      541 ttttctttta gacgcatccg atttttccca tcttgacata ttgatagcaa aggacatacc
      601 tcgcacttgg gatttcttgc cgtgcaatga tatcaaccaa aaaaaatcag tgtatgatgt
      661 gtctttaccc ataattcttg tggtactttc cgcatcagtg tttcttctac ttccatgacc
      721 gtagcatcta gtttgcagat cctcaggcgt tttgagacac gttccacgtg ggtatctact
      781 gcgattgctg gtatcccgaa tgcatccccc aatacgacat ttgctgtctt tctgcctaca
      841 ccaggaagag acatcaattc ttctcttgac gttggcactt gcccatcgaa acgttcaatc
      901 agctgctgtg cacacgcttt gatgtttttt gctttatttc gatacaaacc aatcgttttt
      961 attttaagaa tgatttcatc gattgaagct tctgctaacg catcgggtgt cggaaaagag
     1021 gcaaacaaat ctggcgttgc tttattcact gatacatctg tcgcctgggc acttaagatc
     1081 actgcaatca ataactcaaa tggattattg tgcttgagtt ctccatgtgc ttcgggaaac
     1141 attccataca tcgtttctag agcttccatt gttttttgtt tgcttaacat attccgctac
     1201 tcctccaacc aatttttcag tgagactttc ggcaattctt cttgttgagc tgactgttct
     1261 acttctcttt gaagaagctg ctgttttctt cgtttttggt cttcttccac ttgttgtttt
     1321 gtacgtagat tttttctttc ccacgatagc aaaatgcggt caacgtaatt aaaactataa
     1381 gcctgattta atacggcttc ccgcagtgct agcttcaaaa tttccggctg ataatggtct
     1441 tcttctagcc attgtccgat ccgctggaac tcgatcgaag aaagcgggcg tccaaattct
     1501 tgttcaaata attgatacac tgactggatt tctttttctt gtgttttttc ttcgtatttt
     1561 ttttcttgtg tttttaaata ctcacctaat agatcataga tggggtaaag attatatcga
     1621 tctgcttttt taccgttgac aatcgtcgtt tctatcttga taaatccatt tgttacgaga
     1681 cgatccagta tttgaaaaat ctgatcttgt ttgattccca tatccatagc aatcatctgc
     1741 aaatcaggga aagtatctcc ttcaagctgt gccatatgca actgtagaac aaataaaaat
     1801 tcttcagatt gtagaccgat cctatgataa tttttaagta aaagattaga tatgactgtt
     1861 tgtccagcgc ttaagtaatt ttctaaattc aacatgattt ctcctccgct caatcacagt
     1921 atacgtaaaa aaaacatggg aagcaagaac aggtttcttt tctatttact tcactgatcg
     1981 gtcagattta caatgtcgtc tacttgattt ttgtttgttc atgtttttga tgcgtatctt
     2041 cacttttcgg aataaataaa ttaagtagat ttcgctgttc cttagaatgt tgacaaggaa
     2101 acaaaattac tatacactat ttttgtttcc aagttaacat tattgcataa aggagttttt
     2161 acatgtacag tttcacaaat aaacattcta aagtttttgg atacgtctct gtcttattca
     2221 ctggattagg atttactatc gtcagtcctg tacttccgtt tttaacgctg ccttattctc
     2281 actccataca tcagcaagct ttctatatca ctttgctcat gtctgtttat gctctagctg
     2341 ccttcttatc tgcccccatt ctcggtagct taagtgatca ttttgggcgt catccgatcc
     2401 ttattatcag tttattagga tcaagtatcg gatatttgct ttttggttta ggaaattcga
     2461 tatggatgtt gttccttggt cgtatcattg aaggattgac ggcaggagaa atctccacct
     2521 tatatgctta ctttgccgac atcacggaac ctaatgaaag aaccaaagtt tttggatgga
     2581 tgggtgcttt agtcggtata ggaacaacgc tgggtcctat tatagggggt ctattagctg
     2641 agttggggaa ttctgttcct atatttattg gagcactctt tactttctta aatgccgttt
     2701 atggctacac atttatgcta gaaagccttc caataaaaaa acgttcagta gatttaagtt
     2761 tctcccatgt aagacctttt caccaattac aacaattatt caagatttca tccgttattc
     2821 ctttattgac agcaggtttt gctgtttggt tggcagctgg ctctcttcaa tctatctttt
     2881 ctcaattttc gattgacgcc tttcagtgga aggctgggtt ggttggttta agcttctctt
     2941 tgattggaat cttggatact gtctcgcaat tgttcattat gcctcgattg ctcaaaaaat
     3001 tttcagaaca gcagatcaca agaataggga tgttcagtga aatcctcgcc tatctattca
     3061 tcacattatc tggaattctt ctactgccga ttttgttttt atttgggatt atctgttatg
     3121 gtttcggtga ttctattttt actcctgtat tcaatggcca actatccaat tctgtctcag
     3181 ataaccagca aggtcttgta atgggcggta cccaaagtat acaatctcta tcgcgcgtca
     3241 tcggcccgct aatcgcagga cagctttatg ctgcagcacc atttcttccg acggtattcg
     3301 gatttttgct tatgattttt gcctattatc actacaataa aaaatagtag cttcatttgc
     3361 tacttcgtat aaattctctg tatcgaccga taaaacgaaa tactgtgtca aattcttctt
     3421 ttgacatatt ttcaaataca gtagcatctc gctgtaccca tttttgatgg agttgctcgt
     3481 gtctattcct tattttttgt ccgattggcg tcaattgaaa atagatttct tttttattct
     3541 cttcgctttg ataagtgttt atgatccctc tagctatcag tttttttgtt agcttactga
     3601 tggctcctct agtcatgtaa ctagcattcg ctaattttgt cacgttagga tcagggattt
     3661 tcccgatatg ttcaatcact tctagttcat ttagagtaaa tcctgcaaat tctttttcca
     3721 tttctggttt attacgatga ataatctcat tagctaaatc cagatattct tctattagca
     3781 gttgtctttt atccatttct acaccttctt tttctttca
//
LOCUS       DAZBTF010000147         3812 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000147 DAZBTF010000000
VERSION     DAZBTF010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.154"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..3491
                     /locus_tag="ACHGNS_002890"
     CDS             <1..3491
                     /locus_tag="ACHGNS_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HFN6938749.1"
                     /translation="IFIEVLKFISEVAEKLSKPEYEDIALKMVTPFKENKLWDLDVFD
                     LMNEFLGILSNAQILPTVNWQYISIKDNPKLFLQEFPEELIGETFSTLLQLLETNEND
                     QLVQELANYNEYTGLSFVVDELSDKINILSSSWENRIRTKVFLWWSEQYKSSPIVPKL
                     LKDNSDNWLMKSDKVFLPTDTGVTVLPKELSSWVSLSILKQTYVDELIYQIKTSSSWK
                     NKWEETANAYKAERTGDKRILDAFSEKYLAIEFTEQSSSDLIIGTINRQIDTVEKSIS
                     FINWFYDNYNEKLSAGSELSKVPFNLPNVAGDVQPSNKMYIGKEYGNELGDKLFQDTD
                     YTALKKIDCSSFAEERDFLSFILRCGVSKYPKIYDNDSLKNDVGFKKFIYNKYKNEIK
                     FTNINYLTSKYISNFEELIDKLDTKDIVNWLNQDEELTDLLSSVKKESSASQQSNWSG
                     LYFNSNEYVKYILNKTAWIKIDGEKYSPRQIVLYEKLQAHVPGLYGVSEQSLMELLGQ
                     RIVEQLDFRKNMAMFSDDEIRAILVELPKFDKGEISRRLYNELIRNKKGMNPTYSTEG
                     LSVLAKDGFFYPNKELRYADKRLPKSIEKQMRFIYVPEKASTTTIYEWLGVERFKTNL
                     KLETFELLEDYSEDFKKEIDDIKTAVLSTIDDNSKNVSSLKRIQIIPCSKISAKDVEQ
                     DGQSIVLEDYYFVESKGKFYLKLPANSIEINQLRLIDTFSSTIVDIFKQVLNLSLDLN
                     LIELLISRDTEHKQRKITEEFGVDKWNESFELLFSENALNKKVNDFLLSNGLNEEEYP
                     TIFEIDYSYALKEEEYQLLAIALESISKDIEDLNGLSELIHLDIREFWKLKLRSYLDK
                     KAEHYRQILYSNANKSGDEILKETFLEKIDRYKDYQFEISEIPNSVQVDIEKESKKRF
                     PELSVSITDEIDIDGLYDNNVEELVTVAAITIDDFDYYIRGHQKVSSLLYFGIPSRLR
                     EDILIFVTKENDVDLNGEKSGISIEGTSTVQTILEPSNSVSRKGKNTRNGERSKRDYD
                     NRNSANDDAGETAEQIAYAELTQNQKLNVIWNTKYSSKSSDRNKQPPNGIVCDMWVHD
                     PENGNMYFEVKSSITEFEMSINEYNSMLDNPDSYEVVLVNRDTKEISRHKFDELDGLK
                     QASSYLFKFKQKRSDG"
     gene            3626..3754
                     /locus_tag="ACHGNS_002891"
     CDS             3626..3754
                     /locus_tag="ACHGNS_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFN6938750.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
BASE COUNT         1434 a          511 c          710 g         1157 t
ORIGIN      
        1 aaatatttat tgaggtgctc aaatttattt ctgaagtagc agaaaaatta tctaagcctg
       61 aatatgaaga catagcatta aaaatggtaa ctccttttaa agagaataaa ctatgggact
      121 tagatgtatt tgacttaatg aacgaattct tgggtatttt atctaatgcc caaatactac
      181 cgactgtcaa ttggcaatat atttctatca aagataatcc aaaacttttc cttcaagaat
      241 tccctgagga gctaataggt gagacatttt ccaccttgtt gcaattatta gaaacaaacg
      301 aaaatgacca actagtacaa gaacttgcta attataatga atacactggc ctgtcgtttg
      361 ttgtagatga actatctgat aaaatcaaca ttctttcatc ttcttgggaa aatagaataa
      421 ggacaaaagt gttcttatgg tggagtgagc aatataaaag ttctccgatt gtccctaagc
      481 tattaaagga caatagtgac aattggttga tgaaatcaga taaggtattc ttaccgaccg
      541 atacaggtgt tactgtgctt ccaaaagagc tgtcttcttg ggttagttta agcattttga
      601 aacaaacata tgttgatgag ttaatttatc agattaagac ttcatcaagt tggaaaaata
      661 aatgggaaga aacagctaat gcatacaaag ctgaacgtac aggagataaa cgtattctag
      721 atgcattcag tgaaaaatat cttgcaattg aattcactga gcaaagcagt tcagatttga
      781 tcattggaac gattaatcga caaattgaca ctgttgaaaa atctatcagt ttcattaatt
      841 ggttttatga taactacaat gaaaaactat cagctggaag tgagctgtct aaagttccat
      901 ttaatcttcc aaacgttgct ggtgacgttc aaccttccaa taaaatgtat attggaaaag
      961 agtatggcaa tgaattagga gataagcttt ttcaagatac agattacacg gcattgaaaa
     1021 aaatagattg ctctagtttt gctgaggaga gagattttct gtcatttatt cttcgatgtg
     1081 gtgtttccaa atatcctaag atttatgata acgattcttt aaaaaatgat gttggattta
     1141 aaaagtttat ttataacaaa tataagaatg aaataaaatt tacgaatatt aattatttaa
     1201 cttctaagta catcagtaat tttgaagaat taatagacaa attagatacg aaagacattg
     1261 tcaattggct caatcaagac gaagaattga cagatttatt atcttcggta aaaaaagaat
     1321 caagtgcaag tcagcaatcg aactggagtg gattgtactt taattcaaat gagtatgtga
     1381 aatacatttt gaataaaact gcatggatta agattgatgg agaaaaatat agcccgagac
     1441 aaattgtgct atatgaaaag ctccaggcac acgttccggg attgtatgga gtttctgaac
     1501 aaagcttaat ggaactcttg gggcaacgca ttgttgaaca gttggatttc agaaaaaata
     1561 tggcgatgtt ttcggatgat gaaataagag ctatattagt tgagttacca aaattcgata
     1621 aaggcgaaat aagtcgcaga ttatataatg aacttataag aaataaaaaa ggaatgaatc
     1681 ctacttattc taccgaagga ttatcagtct tagccaaaga tggcttcttt tatccaaaca
     1741 aagaattaag gtatgctgat aagcgactgc ctaaatcaat agaaaaacaa atgcgtttta
     1801 tttacgtgcc tgaaaaagct agtacaacta cgatatatga atggcttggg gttgaacgct
     1861 tcaaaacaaa tttaaaattg gaaacatttg agttattgga agattattct gaggatttca
     1921 aaaaagagat tgatgatatc aaaactgcgg tattatctac gatagatgac aatagtaaga
     1981 atgttagttc attaaaacgt attcagatta ttccatgcag caaaatatct gctaaagatg
     2041 ttgagcaaga tggtcagtca atcgttttgg aagattatta ttttgtggaa agtaaaggaa
     2101 aattctactt aaaattacct gctaattcta ttgaaattaa tcaattacgt ttgattgata
     2161 cgttttcttc aacaatcgta gacatattca agcaggtgtt aaatctaagc ttagatttga
     2221 atttgattga attgttaatt tcccgagata cggaacataa acaaagaaaa ataactgaag
     2281 aattcggtgt agataagtgg aatgaatcgt ttgagctatt gtttagcgaa aatgcgttaa
     2341 ataaaaaagt taatgacttt ttgttatcaa atggactaaa tgaagaggaa tatcctacca
     2401 tttttgaaat tgactattca tatgcattaa aagaagaaga gtatcaattg ttggcgatag
     2461 ctctagaaag catttcaaaa gatattgagg atttgaatgg attgtcagaa ttgattcatt
     2521 tggatattcg tgaattttgg aaactgaaat taagatctta tctagataag aaagcggaac
     2581 attaccgcca gattttatat agcaatgcaa ataaaagcgg tgatgaaata ctcaaagaaa
     2641 cattcctaga aaaaattgat agatacaaag attatcaatt tgagatttct gaaattccaa
     2701 attcagttca agtcgacatt gagaaagaaa gtaagaagag atttccagag ctaagtgtgt
     2761 caattactga tgaaattgac attgatggcc tgtatgataa taatgtcgaa gagttagtca
     2821 cggttgcagc tattacaata gatgattttg attattatat tagaggtcat caaaaagttt
     2881 cttccttact gtattttggt attccgtccc gtttaagaga agatatactg atattcgtta
     2941 ctaaagaaaa tgatgttgat ttgaacggag aaaaatctgg tatctctatt gaaggaacat
     3001 caactgttca aaccattctt gaaccatcga atagtgttag taggaaaggt aagaacacac
     3061 gaaatggaga aagaagtaaa cgagattatg ataatagaaa ctccgcaaat gatgatgctg
     3121 gtgaaacagc agaacaaatt gcttacgctg aattgactca gaatcaaaaa cttaatgtaa
     3181 tttggaatac gaaatattct agtaaatctt cagatcgtaa taagcagcca ccaaacggaa
     3241 ttgtttgtga tatgtgggtt catgacccag agaatgggaa tatgtacttt gaggtaaaat
     3301 catcaattac agaatttgaa atgtcgatta atgaatataa ttctatgtta gataaccctg
     3361 actcttacga ggtcgtttta gtcaatcgtg ataccaaaga gataagtcgt cataaatttg
     3421 atgaacttga tggattgaaa caggcaagta gctatctttt caaatttaag caaaagagaa
     3481 gtgacggttg agtgatttgt ttccacaaac ctattcaatc tatcaactca aaaatcataa
     3541 acaaccatta ttatagtagg aagatacaga gtgtactctc aacgcatatc gaattagtcg
     3601 cttcattcgt taaaggagaa taaaaatgga ccaaaaagaa aaaatgaaag aattgatcaa
     3661 aaagaaacaa ggtggaggcc gatccaaaca aatggaaaca cctaagaatg atcgtaaaaa
     3721 catgagaaaa ggccctaaaa tctttaataa atagacgtgc taaagaaaga ctaagaagtg
     3781 ttaacttttg ttattgtttc ttagtctttt ta
//
LOCUS       DAZBTF010000148         3718 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000148 DAZBTF010000000
VERSION     DAZBTF010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.156"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..353)
                     /locus_tag="ACHGNS_002892"
     CDS             complement(<1..353)
                     /locus_tag="ACHGNS_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938751.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRINESDYNKRDQVLL
                     VKLNEEISVEEAVRT"
     gene            complement(613..1161)
                     /locus_tag="ACHGNS_002893"
     CDS             complement(613..1161)
                     /locus_tag="ACHGNS_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938752.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1162..2019)
                     /locus_tag="ACHGNS_002894"
     CDS             complement(1162..2019)
                     /locus_tag="ACHGNS_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFN6938753.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2305..2591)
                     /locus_tag="ACHGNS_002895"
                     /pseudo
     CDS             complement(2305..2591)
                     /locus_tag="ACHGNS_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2581..2910)
                     /locus_tag="ACHGNS_002896"
     CDS             complement(2581..2910)
                     /locus_tag="ACHGNS_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFN6938754.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2987..3259)
                     /locus_tag="ACHGNS_002897"
     CDS             complement(2987..3259)
                     /locus_tag="ACHGNS_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFN6938755.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3259..3522)
                     /locus_tag="ACHGNS_002898"
     CDS             complement(3259..3522)
                     /locus_tag="ACHGNS_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFN6938756.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
BASE COUNT         1091 a          754 c          473 g         1400 t
ORIGIN      
        1 aatgttctta ctgcctcttc cacactaatt tcttcgttca atttcactaa taatacttga
       61 tctcgcttat tataatcgct ttcatttatc ctaagagtca aatctttttc caccataact
      121 tttacaccca ataaattttg cggtttattt ttctctgttg aaattctttg tgtagccttt
      181 aaactgacaa ttagctcatt gggtacatac tctatttgag agtcatctaa ttggctcata
      241 atcctttctt tttggttagt atctgcagtt acttgtttag tatgagccat gaacccaaac
      301 atactcaccc caacaaccaa tcctaaatat attttctttt taaacttttt catctacatc
      361 tcctcctaat agttttattt tagagttagc tctattactt atttactaac tcaacaacag
      421 attatcatac acttgttaat gaaatcacaa tatctcatta aaacacaccc taaaatcata
      481 accgttccta tgctcatttt ttggattttt taatcacgta accagaaaaa taaatcccct
      541 ctatttcacg caaatcaaag aagaactaag taactaccgt ttaaaggtag ttacttagtt
      601 cttctttgat tattatctaa ctttattatt cattccccac cctttgcgca gtgagtcttc
      661 ggtatcttgt tttatctgac tggtagttct tattatttct ccacgatcat ctaattctgg
      721 aacaattgga tatttttctc tgaacaagtc tagttctttc accacgtcat taaacaaatc
      781 ttgcttatct tcttgcgtaa gattctgttt aatatactca tctaaagctt ttctaagcaa
      841 ttcatctctt gtcatattag tctcatactt ttctactaaa actctggaag tcgcatttaa
      901 caaagtcgac agttttaaag gatgcttggt ggaataaacc ttatcaaact ttctgattgg
      961 tgcgccaact ttttttgttc ttcctctact ctgtctttca acattggagg aagtttttat
     1021 ttcttgaact tggtgcactt ctaattgctt attttcagat ggtgtaattg cttgctgctt
     1081 tttctttgct tctgcttctt tttttatttt ggcgagtcct ttgttgttcc caaaaatatc
     1141 aagtcctttg atgtcagcca attactccac ctccgcatac caattctcgt gttcttccaa
     1201 ttctttcaaa atactgataa ataattcgtg gcttttgcga tcccattgtt ttaagagacc
     1261 tttctttgtc ttctccatcg taatacctga tccgtcgtat cgttttaagc gcgcattttt
     1321 gagtacaaca tttttaatca aatgttcccc ataaatctct tgggcttgtt tatacatttc
     1381 ctgatctact gcgtcaccgc tttccaacat gaaaggaata acaccagcaa catataaatt
     1441 tgtatcaaat tctttcacaa aaaagtccat atactcaaga tattgcatcg catttctcat
     1501 cgattttact tgggtctgta agacgacgat tgcccaatcc gcaatgtaca tcgcggattt
     1561 actatataca gaaatagaag gtgggacatc taaatagatg acgtcaaact catcaaataa
     1621 tggttctagc ttttctttca tatatttgaa ttgggataat gaatcattat attggtcttt
     1681 gatccaatca tcatacattt caaaggacat gtctgctgga agtatataga ggttattgat
     1741 tactttatag acagcctctt taggtggaag attgtttatc aacacgtcag tcacagtatt
     1801 ttcgtaaaca atcggttcac cttctccgcc tccttcttcg ttagtgaagt tcatatgcgt
     1861 taagttcatc acatcagaag tatttccttg catatccaag tcaattatca aggttctttt
     1921 tccttcatta actgctgatt caaaccctaa catttgcgcg cacgtacttt tacctacgcc
     1981 acctttaaaa ttaccgatta catatttctt cgctaccacg ttcctacctc ttttctttta
     2041 gcaatattca tttgatacta ttataacata tttattttta gttactacca aaaaaaagat
     2101 atatatgtac taccttataa ttaataagta aaaggtagta cataaataag gaaaagctga
     2161 aatatattga tttatagctt tcctcaaagt aatgacgtta tttaatttat cgagaagcag
     2221 agaatactcg cgactttagt catgtagacg aatcagattt tcgataaaaa agaagccgtc
     2281 cttcttaaga acgacaacaa atcactagat taattttttc aactgttcat aaaagttttc
     2341 atgcgttcca cataacaata ctggaataac cttgttttct ataatctcgt aagctactct
     2401 atatgttgtg cccgcatatt tgaacccact agaccaaata cctgataaat ccccagtttt
     2461 ttgttcaccc ttatatggat caatcgcaat ttcatcataa attaaattca aaaatttttc
     2521 tttcaatggc ttctctttta gctttttaaa tacttaatga catgttttga aggaacgatt
     2581 tcataagcca atttctgttg ccaactcctc tttagtcatt actttagaat tagtaagtgt
     2641 atcttcagca atatctctaa aggaagaatg cattttgttt tttctattct taaattctac
     2701 aagaatctca tctttatcgt agccttctgc aattatatca gacaaaatat cttcgctaaa
     2761 atcataaaat tctttttgtg gttcaacaaa ttcataaaga ataccattct ctaccaactt
     2821 tgcttcaact tcgactccat taggaacatt aaaatcttta ggtactgtca acatgattga
     2881 attgccttgt tttcttgttt ttgtaatcat ttaaatccct catttctaac agtatgtaat
     2941 tacttagtaa atacattata ccacgagata atgtatttac aagtaattac tgtgactcgt
     3001 aatgcgtcct tgctgaataa atttcaacca cgtgttcatc ttcatctacc ttataaacaa
     3061 cccggtgttg tctattcaat cttctagaat acctaccttc atgtgtaggt tttaacttct
     3121 caaatgattg ggtagggaga tagggatctt cctttaaagt ctgaataacc ttctcaaatt
     3181 gaggtttcaa attagactgt ttgattttct ttaaatcagc tttagccgag tttttaatca
     3241 tcacagaata gttattcatt ataagttatc ccaatcaatt tcatcaacat tagtaaatcc
     3301 actatcatct ttttctcgct tacgaacaac acccaaagtg cctatttgct caagtaatag
     3361 cgtttctttg attgctttcc aatcatccaa accaataagc accgcattat tttcactgcg
     3421 ctcactaatg atttcaattt ccgtatgatt ctcatttaca tctttcaaca acttataaaa
     3481 atttttccgt gctgagctag gatttaagac tgtttttgcc atatctactc cccctctaat
     3541 ttatatctcc attctaacgt acttaattaa gtacgtaaat tcgattgttt tgtttaaatt
     3601 ggagtaccaa aacagcattg taaagtgtta tccgtttcct ttatacccgt ggtgctagtt
     3661 aattttttcc tagaggaaaa tgtctaaaca tgttggtaaa tcaacaaaaa aacgcata
//
LOCUS       DAZBTF010000149         3717 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000149 DAZBTF010000000
VERSION     DAZBTF010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.157"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..155
                     /locus_tag="ACHGNS_002899"
     CDS             72..155
                     /locus_tag="ACHGNS_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFN6938757.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            280..1017
                     /gene="erm(B)"
                     /locus_tag="ACHGNS_002900"
     CDS             280..1017
                     /gene="erm(B)"
                     /locus_tag="ACHGNS_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFN6938758.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1280..2143)
                     /locus_tag="ACHGNS_002901"
     CDS             complement(1280..2143)
                     /locus_tag="ACHGNS_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFN6938759.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2145..2417)
                     /locus_tag="ACHGNS_002902"
     CDS             complement(2145..2417)
                     /locus_tag="ACHGNS_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFN6938760.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2434..2643)
                     /locus_tag="ACHGNS_002903"
     CDS             complement(2434..2643)
                     /locus_tag="ACHGNS_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFN6938761.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2721..3617)
                     /locus_tag="ACHGNS_002904"
     CDS             complement(2721..3617)
                     /locus_tag="ACHGNS_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN6938762.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
BASE COUNT         1143 a          652 c          575 g         1347 t
ORIGIN      
        1 aaattttgta taataggaat tgaagttaaa ttagatgcta aaaatttgta attaagaagg
       61 agggattcgt catgttggta ttccaaatgc gtaatgtaga taaaacatct accgttttga
      121 aacagactaa aaacagtgat tacgcagata aataaatacg ttagattaat tcctaccagt
      181 gactaatctt atgacttttt aaacagataa ctaaaattac aaacaaatcg tttaacttct
      241 gtatttattt atagatgtaa tcacttcagg agtgattaca tgaacaaaaa tataaaatat
      301 tctcaaaact ttttaacgag tgaaaaagta ctcaaccaaa taataaaaca attgaattta
      361 aaagaaaccg ataccgttta cgaaattgga acaggtaaag ggcatttaac gacgaaactg
      421 gctaaaataa gtaaacaggt aacgtctatt gaattagaca gtcatctatt caacttatcg
      481 tcagaaaaat taaaactgaa cactcgtgtc actttaattc accaagatat tctacagttt
      541 caattcccta acaaacagag gtataaaatt gttgggaata ttccttacca tttaagcaca
      601 caaattatta aaaaagtggt ttttgaaagc catgcgtctg acatctatct gattgttgaa
      661 gaaggattct acaagcgtac cttggatatt caccgaacac tagggttgct cttgcacact
      721 caagtctcga ttcagcaatt gcttaagctg ccagcggaat gctttcatcc taaaccaaaa
      781 gtaaacagtg tcttaataaa acttacccgc cataccacag atgttccaga taaatattgg
      841 aagctatata cgtactttgt ttcaaaatgg gtcaatcgag aatatcgtca actgtttact
      901 aaaaatcagt ttcatcaagc aatgaaacac gccaaagtaa acaatttaag taccgttact
      961 tatgagcaag tattgtctat ttttaatagt tatctattat ttaacgggag gaaataattc
     1021 tatgagtcgc ttttgtaaat ttggaaagtt acacgttact aaagggaatg tagataaatt
     1081 attaggtata gggcacctct aataactacc aattaattca cctcaaaatg aaattagaaa
     1141 aaaacagcga aatttttaaa tctatttctt atcgatacaa attcctcgta ggcgctcggg
     1201 accccaacat gaggaaatgt ttttataaaa aaagagtagt taccgttttt tggtaactac
     1261 tcttttcctt ttttaaggtt taaatacctg gaagtttagg tgttttgggt attggtggtg
     1321 taggtagctg caagctttcc attttttgtt gaattgcttt aaattcaggg gtttcttggt
     1381 gttgattttg aaccattttt tgctctattc tctccaaagt cggttgaatt tctttccctg
     1441 atactttacg atttaattct ctttctaagg tctctttcgg actaatggaa ggcgtttcta
     1501 agcttgaata gagtttcact ccttctctat tgtaaagcct tatatcgcta aataagcccg
     1561 ttttatgaag ggtttctaaa ttggtcggta agtttttgac aacaatatca tgcgcttgtt
     1621 ttggtgttgc cctagctgtc attggatcat ctgcatacat ggtttcatat cgttcaatcg
     1681 ttcctaagta tgattcaatt ttcggtactg ccatgacgta tatttttgtt tcataacctt
     1741 tagattgaag cattgtggct gtttgaatgg gaacgtctgt tgttcgtcct gttccttcaa
     1801 tcactaaatt atagccttga tcgcttaaac ggctaataag tgcttctgtc atgcgattcg
     1861 aataaggggt aacgtgtttt actacgtctt tttcatagag tttcactaat tcgtcaaaat
     1921 ttgggtgctg ttgtttaaag gtatcattat caatgacaat aacattccct tgtgtttctt
     1981 caaaaattgc tgatcgcaaa ctggttttcc ctgaccctgg ttgcccacca agtaaaaaag
     2041 cggttggcga ttcaaccgct agtctatttt tagttagtcg ttcaacattc ttttctaaac
     2101 gatcttcaaa ttgcttttcg gtgaagtcag taatattcgc catgttaagc cactttctct
     2161 ttattcaaaa tttgttttga atattgattc attgaccgcc aatactcatg aatagcttgt
     2221 aattcttcaa atggttgttg aaataaatca acttcttgtg ccacttctaa ttgatttaaa
     2281 agattcactt ctagtaaacg agtattttta gtatctagtt catgattcaa aacataattt
     2341 aatacccgat tataaacact tgccgataac tcattaatga tttcaatttc aaatgttttt
     2401 tcatacgtaa ctgccatttc attttcactc cttttaaagt ttgtcaggta aatatttccg
     2461 tatatattct tctaaggctt gatccataac ttcttttacg tttccgccat tctttgctgt
     2521 ttcgattttt atgatatggt gcaagtcggc acgaacacga accgtcttat cccccattat
     2581 atcttttttt gcgctgattg gcgtatcatt tcgttttgct ttctgtgcgc ctaaatttcc
     2641 cacgatcgct cacttctttc tatttcttct tactatttat tttatcatca cctatcacaa
     2701 atcacaagtg atttgtgatt ctattctttt tcattttcta attgaatgat tcgctcaatc
     2761 atttcaaaaa atacgttctc atacatagac aaaacttttt tgtcatagcc tttgtgttct
     2821 gtaatgccat ttttagacca agtacttact ttattacttc gcttgataat atttcggaaa
     2881 accaaattat cctctttatg ttctttgtac agttcttcca ggtttgattt tatcgttgcg
     2941 ctgtccgtat caactaaata aggaacaaaa ccaatcatat ctagtccagg gttaaactgt
     3001 tcttgcaaat caatcaaata ggaaatatag ttttgaatgt tgtttgtact ttcttcttct
     3061 gcttgtaaag ggatcataac gtaatcactc gccacgattg cattatttgt ataaacgctt
     3121 ggcgttggta cagtatcaat aataataaga tcatagtcac tttttaaagg tgctaaaaga
     3181 gtagcaagca atctactttc attttcaaac gtccatgagc gagttaattt tggcagtaac
     3241 atcaaatcaa acgtgccagg gatcaagtct aaattatcag tcaaatgaat aatagaagaa
     3301 gccaagtttc catttttcaa gccttcataa aaattgacac gtggcaattc tacctcaaaa
     3361 gtttttgcta agtcttttgt caatgttgcc tgtaagtcct tatcgatcat taaaactttt
     3421 aaattcaatt tgtctgtcaa gtaagcaaac atagtcgata atttggactt tccaacacca
     3481 cctttaaagt aattattcaa gataataatt gcttcatttt tattgtttaa aatacgtctt
     3541 aattcttcaa gtatttttag tttttccttc tccataacta cgccccattc tttttttgta
     3601 tagtaatatt gtatcattaa gaatcacaaa tcacaagtga tttgtgatta atcacttgtg
     3661 atttgtgata agtgatttgt gattaatata taaaagccct ctttaaaggg cttttat
//
LOCUS       DAZBTF010000150         3702 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000150 DAZBTF010000000
VERSION     DAZBTF010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.158"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..465
                     /locus_tag="ACHGNS_002905"
     CDS             <1..465
                     /locus_tag="ACHGNS_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFN6938763.1"
                     /translation="WKMDETYIKIKGKWHYLYRAIDADGLTLDIWLRKKRDTQAAYAF
                     LKRLVKQFDEPKVVVTDKAPSIASAFKKLKEYGFYQGTEHRTIKYLNNLIEQDHRPVK
                     RRNKFYRSLRTASPTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPD"
     gene            complement(1080..2120)
                     /locus_tag="ACHGNS_002906"
     CDS             complement(1080..2120)
                     /locus_tag="ACHGNS_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFN6938764.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(2469..2795)
                     /locus_tag="ACHGNS_002907"
     CDS             complement(2469..2795)
                     /locus_tag="ACHGNS_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938765.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2782..3570)
                     /locus_tag="ACHGNS_002908"
     CDS             complement(2782..3570)
                     /locus_tag="ACHGNS_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN6938766.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
BASE COUNT         1135 a          636 c          553 g         1378 t
ORIGIN      
        1 tggaaaatgg atgaaacgta catcaaaatt aaaggaaaat ggcattattt gtatcgagcc
       61 atcgatgcag atggtttaac cttggatatt tggttacgta aaaaacggga cacacaagca
      121 gcctatgctt ttcttaagcg gttagtgaag cagtttgatg aaccgaaggt tgtagtcaca
      181 gataaagcac catcaattgc tagtgccttt aagaaactaa aagaatacgg attttatcaa
      241 gggacagaac atcgtaccat taaatacctg aataatttga ttgaacaaga ccatcgtcca
      301 gtaaagagac gcaataaatt ctatcgaagt ttacgcactg cctcacccac gattaaaggc
      361 atggaagcca tccgaggatt atataagaaa acccgaaaag aaggcactct cttcgggttt
      421 tcggtctgta ctgaaatcaa ggtattattg ggaatcccag attaaatcat agataccgta
      481 agggatttta ttctttattt aaaactttgc aacagaaccg tccgttgcgg gtaataccgt
      541 aacactgaag ggcacgcatt aaggcgtcat cttaatgctt tcctgtagga cactgacgga
      601 gattaccagt ctcccgagcc ttacaggggc ctttcttttt ttgtattttc aattatcaca
      661 atttatcgtc caaaagtgca gaacgaaaat caattcatca caagcatatc agacaattaa
      721 atttaaaaca actaaaaata tacaaaaaag tgtaattgaa tattagcagc actgcctgaa
      781 aagaagtaat atcaggcttt tcagcttggg gaaataagtg aattacctct cttcatgaat
      841 tattataact caaaaaagca catatgcaaa cactagttcc ctattttttt gaatgaaaaa
      901 taactaaaat atctaacgat tatgacttta aaacctacgt ttgcgattgt tttcataaac
      961 ttttaatcta aaaactccgg aataatagct atttatttgt aacatttgta ataaaattaa
     1021 aatcataaaa aagttctaat ttaaaattga tcatttttat agtgatcgct ttttatcaac
     1081 tactctagcc aattttccgt actaactgtt ggtaattcgt taaatgtttc tctttgttcg
     1141 tttgcaagct ttgccgcaat agctttttcg acaaaccaat tttttacata aacgaaaatt
     1201 agattctgca aattgtttac tttttcagtt ttttgttttt gataagcttt cagcaaagtc
     1261 atatataatc ctttgtcagc atcaattcca taagattcca gttcttcata aacaacaact
     1321 gtttgctcta attcttcagc ttttttcttt gcattatgaa tagtttggac ggttttttga
     1381 gcgtcttcgt aagtactaga aaatgttttt atcaagttta atacattttc tggtacaaat
     1441 gtatttatac tgtcctcttt catgatgtcg acgaagttat ctagtaaaac ttgattttgt
     1501 aactgatcag tttcagtgtc tattatgtgt ctattagtgt ctatagaatt agaatagaat
     1561 agattgtctg cgattttcgc aattacggtt gagtccttga tatgactgga ttttgccgta
     1621 tttgcgattt tcgcaattac ggtggtatca ttgatttctc gcggttttgc cgtatttgcg
     1681 attttcgcaa ttacggtggt atcagtattg tcttcctctt ccatgatttt aaagacgtct
     1741 tgattcgtta cttctggctt gcctaagtac aataaattag gcatggattc aatcttaccg
     1801 tcacgtttat ttactcttcc tctcttctgt ttcagaagac ctgctttttc taattccttt
     1861 ttgattttag atactttacc ttcgcggcat tgtaacaatt tcattaattc ttcaactgtg
     1921 aatatgaaaa atatattatc atcagaatct acccagttat ttttgactga ataatcaaat
     1981 cgatctttaa gtagcatata agcaattttt gcatcatttg atagatccat gtatttttcg
     2041 ttagtaaaaa ataccttagg catttgataa tatttttcac gataaatttc ttttgatttg
     2101 taataattaa aatcattcat agccaatcct ctttctgtag gatagcgttt tgttcaaaac
     2161 tatgttacaa tgaattaaac aaatatatct atccactata tatttttcta ctgctgataa
     2221 gtcgccaaac tttgttaaag cagtagtctt tttttatctt tttttagttt accttaaaaa
     2281 ataaattaaa gcaaaccgct ctatttcaag gtttaagctc atagtaacta tatcacgata
     2341 ttacaataat attatatcac gatatcgtaa tattacaata tgaagataag cgacttatca
     2401 gctttgatac tactgatttt tataaaaaaa atatcacgat attatgatat cgtgatattg
     2461 aatgtaaatt aaattttatt ttgtttcttt gctagtttat atccttcaat caagtattct
     2521 tcaggtatgg actttaatag cgcatcttct ataacgtctg gtttactttt gtctgattca
     2581 gcaatagtcg tccaatattt gagtgctgac attagaattg gatttatacg aatgctatct
     2641 gctgttctta cactacttcc ttgatctttt atgactttac ttatattttt cttggttaat
     2701 tcgatttctt ctacttcttt tgatttaaaa ttctttttat ttttatttcc tagtaatcta
     2761 cttactgcag cttgactgcg attatcttct gccatcaaaa tcaccaactt cttttaaata
     2821 ttcttctgta aatgctttat agtcatcttc agctgttgag ggtttattaa aattgattag
     2881 atctgatcca gcagcttgcg cttcgtcaac ggttattgtc ctacgaatat aagttttgaa
     2941 aatttttgca ccaatgactc tagaaatttc ggttgctgca gacgtcattt ctttagaaat
     3001 gttataacgc ttattatact tagtaaatag aactcctcgt acttcaagct tgtcgttcgt
     3061 atattctttt accgtttcaa tagtttcact taactttatt agtcctttta atgagaaata
     3121 cgcagcttca actggaatga taatttcatc agaaacagta aatgcgttga ctgtaagcat
     3181 tcctaaactt ggtggacaat caattataat ataatcatag ttgtccttga ttggttgaag
     3241 tactctttta agcctgtgtt cacttcctac ttgagtaaat tccctctctg cagaagaaag
     3301 ggctatgtcg gctggcaaaa catcaattcc tttatagtcc agaatagcat ctgtaatgtc
     3361 agcctcccct tttaatactt cataaattgt aggttggttt gttccatcta tatcgaaacc
     3421 tacattatct gtagcatttc cctgaggatc aatgtccact aaaagtacat ttttaccgta
     3481 aacaaatttc aaacatgctg ctaaattaac gctagtcgta gttttagaaa ctccaccttt
     3541 ttgattggct actgacaata ttcttgccat taaaatatcc cccattcatc ttatattata
     3601 atataattat atcataatat tgtgatattg caatatcgta tatgcattat attgtaatat
     3661 tgtgtgatta ttttgtttta aagatatata tcggttcgga ta
//
LOCUS       DAZBTF010000151         3542 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000151 DAZBTF010000000
VERSION     DAZBTF010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.159"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            52..1041
                     /gene="galE"
                     /locus_tag="ACHGNS_002909"
     CDS             52..1041
                     /gene="galE"
                     /locus_tag="ACHGNS_002909"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFN6938767.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            1053..2537
                     /gene="galT"
                     /locus_tag="ACHGNS_002910"
     CDS             1053..2537
                     /gene="galT"
                     /locus_tag="ACHGNS_002910"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFN6938768.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            2561..3541
                     /locus_tag="ACHGNS_002911"
     CDS             2561..3541
                     /locus_tag="ACHGNS_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN6938769.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
BASE COUNT         1204 a          646 c          775 g          917 t
ORIGIN      
        1 aattatatat aatcaacaaa cacaatttta ctaatcaaag gagtgttttt tatgacaatt
       61 ttagtactag gcggagccgg ttatatcggt tcgcacgctg tagaccagct agttcaaaag
      121 gggtatcaag tagctgtggt agataatttg ctcacaggtc acaaacaagc agtacatcca
      181 gatgctcatt tttatgaagg agacatccgg gataaagaat ttctacgttc cgtatttgaa
      241 aaggaaccga ttgaaggagt aatccatttt gctgcaagct ctttagtagg agaatctgtc
      301 gaaaaaccat tgatgtattt caacaacaac gtatacggta tgcaaatctt acttgaagtc
      361 atgcacgagt tcaatgtgaa taaaatcgtg ttctcttcta ctgcagcaac ttacggagaa
      421 ccaaaagagt ctccaatcac agaagataca ccagctaatc ctaaaaatcc gtatggtgaa
      481 agtaaattga tgatggaaaa aatgatgaag tggtgtgacc aagcatatgg catgcgctat
      541 gtcgctttgc gctacttcaa tgtagccgga gcaaaagcag atgcatctat cggagaagat
      601 catacaccag aaacgcatct tgtcccaatc attttacaag tagctcttgg tcaaagaaaa
      661 gcattggctg tttatggaga cgattatgat acaccagatg gaacatgtat cagagattat
      721 gttcaagtag aagatttgat tgctgcacat attttagctt tggaatactt gaaagaagga
      781 aatgaaagca acttcttcaa tttaggcagc agcaaaggat actcagtcaa ggaaatgcta
      841 gaagcagcta gagaagtgac aggaaaagaa attccagcag aaatagcacc aagaagagct
      901 ggagatccta gccgattagt cgcttcaagt gaaaaagcga gagaggttct gggttggaaa
      961 cctgaataca cagatatcaa agcaattatc aaaacagcat gggattggca tgtcagccat
     1021 ccaaatggtt atgaagaata ggggaatgag atatgtcaat cagtcaaacg atttgtgatt
     1081 ttgccacact tgcgatcgaa gctggcggat ggatggaaat ggaccgctta tatttacaga
     1141 atcgtctttt agggatggta ggagaagatg ctctagaaag cattgaagta agaccagtta
     1201 aggaagaatc tgtagactta ttggatcagc ttgtcgctaa ggcaagagaa aatggtgttc
     1261 tttcagaatt gccagcagaa caagagattt ttgaagcgca attaatggat tttctcacac
     1321 cgccaccttc agtcgtcaat gcattctttg cacagcacta ttcgaaaagc ccagaggaag
     1381 caactgagta tttctttaat ttaagtaaga aaaatgacta catcaaaaca agagccatcg
     1441 caaaaaacat caaatttccg gcagagtcgg agtatggtga actggaaatc acaatcaatt
     1501 tatctaagcc agaaaaagat ccaaaacaaa tcgcagcaga acgcgaggcg gtaaaagtag
     1561 actatcctac ctgcatgctt tgcatggaaa acgaaggata tagaggtcgc ttgaactatc
     1621 cagcccgcac caatcaccgg attattcgaa tgaatctaga cggtgaaagc tggggattcc
     1681 aatattctcc ttatgcttat tacaatgaac attcgatcat tttatcagaa caacaccgcc
     1741 cgatgaaaat cacaaaggaa acatttgcta gactgcttcg gattactgaa gtcttgcctc
     1801 attactttgt tggttcgaat gctgacttgc cgattgttgg aggatcgatt ctttctcatg
     1861 atcattatca ggcaggacgt catgaatttc ctatggcaaa agcaccgata gaaaaactta
     1921 tcaaactagc agattatccg caagtcatcg caggagtcgt gaaatggccg atgtctgtga
     1981 tccgtttgca atcaacagag aaaaaattgt tgattgaagc agctaactca gtattggaaa
     2041 agtggaaagc atattcggat gaaacagtat ctatcagagc tttttcagca gatggtacac
     2101 cacaccatac gattacgcca attgcacgaa gaagggacaa ttactttgag ttggaccttg
     2161 tccttcgaga caacaatgtt tcagaagaac atcctgatgg gatttttcat ccacataaag
     2221 atgtccaaca tatcaaaaag gagaacattg ggttgatcga agtaatgggg cttgctgttt
     2281 taccacctcg tttgaaacca gaactagtag aagtagagca tttcttatta ggagaagaaa
     2341 accagatggc tgaatatcat cgtccgtggg cagaacaatt aaaaaaagaa catcctgatt
     2401 tgacgaaaga tacggtcacc gatgtcgtac aaaaatctgt aggtcaggtt tttgcacgtg
     2461 tactggaaga cgctggagta ttcaagcgag atgaaaaagg acaggcagcg tttgaacgat
     2521 ttactgcaat tttataaacg taaaaaagga ggagagacaa atggcaacaa ttaaagacat
     2581 tgcgcagttg gcaggagttt ctcctgctac tgtttcaaga gttctcaact atgatcctga
     2641 tctttccgtt ggcttagcca caaagcaaaa aatttttgaa gcagcagaac gcttaaatta
     2701 tacgaaacat cgtaaaaatg cagcaaatac taagcaaacg cttcgcctga tccagtggta
     2761 cgacgatacg gaagagcttg aagatcttta ttatttatct attcgtctag gaatcgaaaa
     2821 aaaggcagaa gaagcaaacg ttactttatt gaaagaaacg tgggattcca ttagtgatct
     2881 tccgacagat ggaacgatcg cactaggaaa attcgacgaa gctcagttat tagaaatgaa
     2941 aggatcccaa aatgccttgc ttttggtaga ttctgacggc acttctcaag gaatcgactc
     3001 tctagttgtc gactttaagt caagtgttca aaaagtcatt gaacattttg tctcccataa
     3061 agcgcaacat atcgctatgc ttgctggaac agaatatacg aagaaaaacc atcagcggct
     3121 aaaagatccc agaattatgg catttcaaca agttttggca gaaaaaggct acgacgaagg
     3181 tccaattatc gaagcagatt tttctgtcga gtcaggatat caagcagtga agcagtattt
     3241 aaaaacgaat ccgcatgttc ctgatgcgct ttttgcagct aatgatgcat tagcgattgg
     3301 cgcactgaaa gcgattcaag aaaaaggttt agtggtacca gaagatattt ctgtgatggg
     3361 tttcaatgat gtcagtgtag cgaaatatgt aacgccctct ctttctactg taaaagttta
     3421 taccgaatgg atgggagagc tagcagtgga gacgattctt caattaagta attccaaagc
     3481 accggtttca agaaagatta ttgtggaaac agagttgatt attcgagaat caacgaaata
     3541 aa
//
LOCUS       DAZBTF010000152         3532 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000152 DAZBTF010000000
VERSION     DAZBTF010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.160"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(266..970)
                     /locus_tag="ACHGNS_002912"
     CDS             complement(266..970)
                     /locus_tag="ACHGNS_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938770.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(1157..1402)
                     /locus_tag="ACHGNS_002913"
     CDS             complement(1157..1402)
                     /locus_tag="ACHGNS_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938771.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(1419..2270)
                     /locus_tag="ACHGNS_002914"
     CDS             complement(1419..2270)
                     /locus_tag="ACHGNS_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFN6938772.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            2436..3440
                     /locus_tag="ACHGNS_002915"
     CDS             2436..3440
                     /locus_tag="ACHGNS_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFN6938773.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         1096 a          602 c          683 g         1151 t
ORIGIN      
        1 ttttttctct atcagaaaat atgtttatta ttagaatttt ttatagttaa ggttaggcat
       61 cttaagaaat caggcaactc caagatatct tcaggtagaa gagaagtcgc ctaacattgt
      121 aggatcatag gtgatttgtt ggacaggtca agctgatatt tgagcctgat gttagaagga
      181 atttcttttt acttgctgta gaaattatta tatgactaaa aaggctggga ttttaaatcc
      241 cagcctttta tgacaaatag aatttttatc gatcagtaat cgctgtcgct gcaaattgtt
      301 cgatcaaagg aatcaaagtc aatttgactt tgtatttccg ttcgagtgct ttttcgataa
      361 cgaactcatt ttcagtagtc atagaaatag ttgctaattt acgaggttca tcagcatttt
      421 tttcattaat aactacttca tgttcatttt gttctgtgta aaatttagga tactcagcaa
      481 cggcttttga aaaatcagca aaacttaaag cggcattcgt atcagttgag cgtgtatttt
      541 cagttgtgtc cgcttttggt ttttccgttt tttcttctgg cacactgact gtttcttctg
      601 taactggtgt cgcaacagct tgcgcagttg gttcatcgtt tttcaatcgt tgatcattcc
      661 agtaagacac gatttcaata agaagattgt cacggaatcg ttcgaaaatc ccgttttctc
      721 ctgaacgaat tctctttaat acagtattga agttgtcatc gattggcacg ttgatccgtg
      781 tatcttcatt tgttttccca gctaatccga tcgttaagat ttgacggttt ttgggatcaa
      841 gcatgacttg taacggcaaa ccataacgtc gcaagctctc ttcttctaga gaacggaata
      901 attgtcctct tacaccatcc aatgaaggac ggaattgatt taataatttt tgtttctgtt
      961 ttttcttcat atgttctctc cttcgctcat accctttcga ttataagaaa aaaacagcca
     1021 aatgaaaagg agtaatgcaa aaaaacttgc gtatacgcaa attacaacag aaaaatttgc
     1081 gtattttccc gttttttgaa agaaaaataa aaaatggaac ggattatgta tatatacata
     1141 atccgttcca ttctttctat tttttcggta tcaaggcaaa aacaggtagt tcaggtgaat
     1201 aaatgacttc atgttcgtgc cagggtttga ccattagcga atgatcgaac cgttttttca
     1261 gttcttcgat gatttgatca tgtgtccagt ttcttgcagg aaaatgttgg attttctctg
     1321 catcgatcaa aaagacaaaa tcggaatgaa aagtctgggt aacttcttcc gagagagaat
     1381 tcaagcgttc gattggatcc ataaaatcac ctttcgactt aaaattgggt agtatctggg
     1441 tcttttgctt tgccgactac accatctaac tgatcgattt gtttcatttc ttcttctgtt
     1501 aattcaaaat caaataattc agtattttct ttgattctgc ttggtgtcac tgacttagga
     1561 agaggaagga attcgtgttg cagcgaccaa cgcaaagcaa cttgtgcaat cgtcttgtca
     1621 tgtgcttcag cgatttgttt catttcaggc acatcgaaga ttttaccagt gcctaatgga
     1681 ctatatgctt cgaggatcat atcatgtttt tttgcatact caactacagt tgtctgtaat
     1741 tcgcctggtg caaggaaaac ttgattgacc atcggcataa tttttgctgt ttttgctagt
     1801 gtatctagat ggtggggtaa gaaattgctc acaccgattg ctttgatttt acctgcttca
     1861 tacagttctt caaatgctcg ccatgtatct gcatttgctt gttcccaatt atcacgaaaa
     1921 gcaacagggt ttggccagtg gatcaaaaac agatctaaat aatctgtttg gagcttcttc
     1981 aatgattctt caaaagaaga catcacaagt tcatagctgt ggtttgcatt ccataatttt
     2041 gttgtcaaga atatctcttc tctaggaatt ccactttcct taatcgcttg gccgacgctt
     2101 tcttcattct tgtatccttg ggcagtatcg atatggcgat aacctgcagc cagcgcttct
     2161 ttaacagagg aaacagcgac atccccatca ggtgtttgcc atgtgccgaa tcctactacc
     2221 gggatttcgt aaccatttga tagacgataa gtgcttgtta atgaattcat actttcagct
     2281 ccttttttat tcgttcaact ataagtgtag cgcatttttc gaaaaaaaga atccattttg
     2341 tttctactct tgaaattttt gtttaaaaga aaatgatttt gattctggta tttttgattt
     2401 tttttccgta aactaagaca gaagggaatg agaaaatgga tatctattta aaaaaagcaa
     2461 ttctgcatat tattgacaga gaaactggtt ctccagtctt ttcacaaaaa gagctggatc
     2521 tgacaaaaga atatattcgt gattttcttc aaaagaagat ccaaaaaatc tcttctgctc
     2581 aaacaaaaac tggacaacta gcagaagaaa gcatgatcgt tgaatcactt aaaatcgctc
     2641 aagaaaattt tgtggaagca agtgaagcaa tcgttcagcg gtggttcgat atctaccaag
     2701 aaagtgaaga agcacctagt gcagatgtat ttgttgtctt gtatgaatta gatacagtga
     2761 tgtacttatc tttattgaaa gtcaattatc gtgaggctta cactcacttt gttgaagcag
     2821 acgaagcagg gatcgacaac cgcttgattt tgaatcgagc aattctaggg gcgaaaacac
     2881 aaaaagcaga tgaagcattc gctgtgaatt tgactgattg gacctacgaa ttaattgaaa
     2941 aaaaatatga gttttctggc aaaaaagagc tttatttttc tggaagagtg atcgaaagtg
     3001 ttcctgcacc ttctttagaa gaaaatgtaa aagtgatcca aaaagtagcc aagcatcttg
     3061 gaaaaaaatt tgagacagaa gaatttgata tcatggctga tttgaaagaa gctgtctatg
     3121 atacgataga agaaaaaggt cggttggatc atgagatgat tgcggaaaaa gtcttcaagg
     3181 aaaacatcac ggcaaaaatg gcttttaaag aagaagtaca agagcaagga tttgtcccag
     3241 aagcacctcc tgtaaaagaa gtacaagaaa tttctgagaa aaaatttgga aaacaaaaat
     3301 tcaagctatc aaacgggatc gaactgatcg taccagtaga tgtatatcgc aacccagatt
     3361 tgattgaatt tgtgaataat ccggatggta cgatatccgt gatgatcaaa aatgtggatg
     3421 aagtattgaa tcgtttataa aaatgactag aatattaggt gttcaagtac cagtgatcag
     3481 ttaaatgaat tgtgtcaaga gaaggatcgg aaaagacgaa tataacaaaa aa
//
LOCUS       DAZBTF010000153         3484 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000153 DAZBTF010000000
VERSION     DAZBTF010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.161"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            151..384
                     /locus_tag="ACHGNS_002916"
     CDS             151..384
                     /locus_tag="ACHGNS_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFN6938774.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            381..788
                     /locus_tag="ACHGNS_002917"
     CDS             381..788
                     /locus_tag="ACHGNS_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFN6938775.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(1925..2443)
                     /locus_tag="ACHGNS_002918"
     CDS             complement(1925..2443)
                     /locus_tag="ACHGNS_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFN6938776.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            2810..3058
                     /locus_tag="ACHGNS_002919"
     CDS             2810..3058
                     /locus_tag="ACHGNS_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938777.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         1183 a          590 c          526 g         1185 t
ORIGIN      
        1 aagggagcaa tctaaatttt ttgaattttc ttaattaatt atatagaaag aacaatcttt
       61 ctatagagtt agacatgaga gggactatgc ttgatttaaa atcaacaagc gtatatactg
      121 tatatacaaa gaaaaaggag ggagaagact atggcaatta aagaaagaaa atttagggaa
      181 gtggggaact cagtagtatt aactatttca aaggagtttc ttaaagaaaa tgggttaaat
      241 cccggagata cggttttttt agatgaagaa aaacttcgag cggcaattac aaaaaaacaa
      301 gccttatctg atgaaattga tttagcaata tctcaagcat taactgaata tgatactact
      361 tttagagagc ttgtcgataa atgagtattc attacctaga agccaaagat attataaaaa
      421 tgaatgtaat ccaaataaaa aaatactctc ctaatgaacc ccttggtgta aaggatgcga
      481 atgctttaga aatgtgtgta gggcaacttc gagcctcagc ttttggtgaa gaggtttatc
      541 cttcgattta tgaaaaagca gctatcctac ttatacaatt gataaagaaa catccttttc
      601 acaatgcaaa taaacgtact gctttcttag ctacttttgt ctttttgaaa atcaatggac
      661 agcttttagc tattgatcaa aaagatgctg taaatttagc ggtatatatt gctacgtatg
      721 ataaggattt cgatcaacta aaatatgaag tgatcaacac tataaagaca catacgcagc
      781 ccatttaagg agttactgta tgtgtcttta tagggttatt agctgctata gcgaaataat
      841 tgaagtaaat tacaaatagc tataattcaa atccaactat atagaaaacg taaattttct
      901 atatagttgg attaagataa cgactattta ttttatcata tttttactat accctatact
      961 tttacttggt atatttatga ataatcgcaa atttacccct atactactag tttgtaagtt
     1021 agattaataa aaatactaaa gttatttgtt cttttattaa atacacctac aacttataaa
     1081 ttgatggtat ttagataaca cgaaagaata aaaataacag ctgatagtaa agtttgttga
     1141 cgattgctac tcaatcgaaa gtgagttagt gcgtatgaac ttgcttctga aaacttatga
     1201 aatgaaagac cttttacatc ttgcagaaat cgttactggg attaaactaa gcaataaaaa
     1261 ctaatgttcc cctcattctt tgaacttcta ctatttgatt tatttattct gataaatgac
     1321 gatttcacct tcaggatctt caaatcccgt atagaattta tttaagatct cttcagtatc
     1381 agtaatagga taggcgatag gccacatacc ctttgatggt tagaataaac tgctcaaaat
     1441 ctgtatcgta tgttggcttt agcatattaa caaaggctat ctccaaatta taaaccataa
     1501 gcgttgaatc gttcttaaca atttcaattc ttcctaaccc aaagtcagaa gatataatta
     1561 ccggacttgc cttacccttc atgtgggttg ttgagattcc atatttaaag ggaattgtcg
     1621 gtactatcgg cggtctgaaa aacatcccaa aaataaaaaa atctttcccc tgctagtctc
     1681 ctaagtccct ttgtagcgga tatggcacca taatctcatt ttatcgaagt gagtcatggt
     1741 ttatctgttg gccaaccaaa attgatacaa tctaacgatg caccctctac ggcattctag
     1801 gggagtgcac ttgcattcac ccaataaaaa ccggcagtta gcaaagaact tatgtagtcg
     1861 tatgttctta ctgttaacat acaagaggtc tcctttccac aattgcttag cagtaaacgg
     1921 gaatttacaa agctaatctc ttccattttc ttatctttgt tctaaaagtc ccaaatggag
     1981 caactgtatt tacatgaata aatttatata cttcccatac agccgttttg gttgcctcgt
     2041 ctgcccattt tcttttgtgt gcagtgaata gatcttcatc actcattgaa tcaatcatca
     2101 tgttaatatc atcaatatta cgattgagaa tttcctctaa ttctttcaat ggtagatgtg
     2161 cataggtatt tgtaaaccac ttatataact ctcctaactg attccattta aattgatcag
     2221 aaggcgtttt aacagataat ccatttcttt catcattttc ccattttaga attaaattcg
     2281 tccaaccaac ttgatatgct aaattttcag caggtgttct atccacttcc tcaactcttt
     2341 tatccttcaa ttcttcagga atagtttcaa attctaaaat atatttagta taagtcctat
     2401 ttatttcttt ttttaattct tctttatttt tatatgttct cattatatta cctccataaa
     2461 ttcctaattg tctcctttaa ttcatctata atttcatcca cgatatttta atccgttcta
     2521 caatgcatat ctgatatgca ttgactgtct aactcatatg tgaatgattt ataaaatatc
     2581 ccaacgtctc atctaaagaa atgttgatcg tcagcaagga gctaaaaaat acgaatatat
     2641 ttcttttgta gtaaaatcgg actggtttgc atatcatttt cttcgtaaat atcaaaatca
     2701 tgttaactac tatcaattac taataaaaac taccacttta agcgttaaga actgttaagt
     2761 ttagttaagt gaggatagtt cacaaaatcc attgttaaac ttcacgaaag tggtaaaact
     2821 caaaatgctt tatataggat tacagcatcg ctctttcttc gattgcgcgt tgggtaaaaa
     2881 atattatgaa gtaaaaattg acgatgacac gttccttacg gctcatcaga tgaaacagct
     2941 acaaaaacga aatgatcaac tcgaagaaaa tttaattcta aaaaagcgat tgccatattc
     3001 acgcctcgct cgacgaacta ttaaaggtca agatttacct gaagatcatc ctttttagga
     3061 aattgaagct ttttatgttg cacaatgcat ttataatact acaatgatgt tttcaccaga
     3121 tattcttcta gggtgcggtg taatgaaaca gatacacttg caaaagaaaa ttcaccgcga
     3181 atttactaaa attatgaata attatgttaa tcttccgaaa tttttttcca aaattaaggg
     3241 atcaagctgg tgttatagat tgtttaaggt tggcaaaaaa ataacttctt tttaaacaat
     3301 tatagaaaac cgatactctg ttaagttggt tgacacggtg tcgattttta tgactttctt
     3361 attctgaaac ttcactaacg gacagtacct aaaaagtcta tagtatggac gtttacgagt
     3421 actattttat caaaaatgct gctcatttca acttatatgt tttagtgaac tacccccact
     3481 tagc
//
LOCUS       DAZBTF010000154         3388 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000154 DAZBTF010000000
VERSION     DAZBTF010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.162"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(63..2555)
                     /locus_tag="ACHGNS_002920"
     CDS             complement(63..2555)
                     /locus_tag="ACHGNS_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFN6938778.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(2562..3029)
                     /locus_tag="ACHGNS_002921"
     CDS             complement(2562..3029)
                     /locus_tag="ACHGNS_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFN6938779.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(3165..3238)
                     /locus_tag="ACHGNS_002922"
     tRNA            complement(3165..3238)
                     /locus_tag="ACHGNS_002922"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3202..3204),aa:Arg,seq:acg)
     gene            complement(3296..3369)
                     /locus_tag="ACHGNS_002923"
     tRNA            complement(3296..3369)
                     /locus_tag="ACHGNS_002923"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3333..3335),aa:Pro,seq:tgg)
BASE COUNT          924 a          750 c          557 g         1157 t
ORIGIN      
        1 cttgtcacaa cctcttaatc aagtcttact attataaaaa ttcagctaca caggccaagt
       61 aactataggg aacctactgt ttccttttta ggtccgcgaa cattcaatgt gatcttacct
      121 tttgaagcac ctactgttac gtggtcacct aaacgaattt ctccgccaag caaagcctcg
      181 ctcaatcgat cttcaatttc tttttgaagt gcgcgacgga ttggacgtgc accatattca
      241 ggatcaaatc cagctttccc gatcacatcg attgcagatg gtgtgatttt cacttgaata
      301 tcttgttctt tcaatcgttt gataatcgct ttgctcatga tttggacgat tgtatggatt
      361 tcatctttac tcaatgaatg gaagacgaca gtctcatcga ttcgattcaa gaattcaggt
      421 cggaaagctt tctttaactc ttctaagata cgtttttgca tggctttgtg atctttagta
      481 acatcttgga cattgaatcc gacatttttt tcttctcgaa tttgtgtcgc tccgatattt
      541 gaagtcataa tcatgattgt atttctaaag tccacttttc tacctttaga atctgttaaa
      601 tgaccatcat ccaatacttg cagaagtaaa ttgaaaacgt ctggatgtgc tttctccact
      661 tcatctaaga gaatcactga atatggtttt tgacgaattt tttctgtcaa ctgtccacct
      721 tcatcatacc ctacgtatcc tggaggagac ccgattaatc ggcttgtact gtatttttcc
      781 ataaattctg acatatcaac acggatcagg gcatcttcac ttccaaacat gacttctgaa
      841 agtgctttgg ccaattctgt ttttccgaca ccagttggtc caaggaacat gaatgaacca
      901 attggacgat caggatcttt caatccgctt cgtgcacggc ggatcgcacg tgaaactgct
      961 ttgacagcct catcttgacc aacgacacgt tcatgcagaa gtccttccag ttctaacaag
     1021 cgttcgcttt cttttttctc caactgctgt agagggacac cagtccactc cgatacaact
     1081 gtagcaacat cttcttctgt tacacgatca gaataacctt gctcctgttt tgcttcaatt
     1141 gcagaaactt tttgcagctt tttacgtagt gctttttctt gtctgcgtaa ttgagcagca
     1201 ttttcaaagt cttgtttttg gatcgctgct tccttttctt gtactagttc atcgatttct
     1261 agtttgatca cagttgattt tgttaagtga tcggtttgat ccaaacggac tttcgctgct
     1321 gattcatcga tcaaatcaat tgctttatcg ggtagttgac gagaagtgat ataacgaaca
     1381 gaaagattga ctgctgctcg tacagcttca tctgtgatct ccactccatg gtgctcttca
     1441 tatcttgtac gtaatccttg aagaatcact tctgcttctt caggagttgg ctcatctact
     1501 tgaatgcgag caaaacgtcg ctctaatgca gaatcttttt cgatatattt ttgatattca
     1561 tctagggtcg ttgctccgat cgtttgtaat tctcctcgag ctaacgccgg tttcaagata
     1621 ttcgaagcat caattgctcc ttctgctcca cctgcaccaa ttaaggtatg caattcatca
     1681 atgaataaga ttacttgacc atcattatag atttcgtcga tgaccttttt cattcgatct
     1741 tcaaattcac cgcgatattt cgtaccagca accaaagatc ccatatcaag catcatcagg
     1801 cgcttttctt gcatgtcttc tggcacttca cctaaaatga ttttttgagc taacccttca
     1861 gcaatggctg ttttgcctac accaggttca cccaccagta cagggttatt ctttgtccgt
     1921 cggctaagaa tctggataag ccgtttcact tctctcgaac gaccaacaac tgggtccagc
     1981 ttgttttctt ttgctaattt cgtcaaatca cgtgctaatg aatctagcgt aggtgttcct
     2041 tgcggtgttt gccgtttatc ttgtacattt acatttctac gacgattcat tccttttcca
     2101 gcagaatcac cagctagccc cattttttgt ttcagaactt gacgggcttt tgaaaggctg
     2161 atacctaagt tgatcatgat tcgagtagcc atgatacttt catcttttaa taagctcatc
     2221 aaaatatgtt ctgttccaac tttttgagaa ccagttttct tcacttcgct gctagctaaa
     2281 gcaaaaactt gtttcattct tggtgaatat ggaagaaaga ctcctttagg gtaagaacgg
     2341 ataccaccat aaccaatcag atgttcaatt tcttcataaa tttccgtttc attcaaatcc
     2401 atttcacgta atacttttcc agcaattcca ttttgctcga ccactaaggc tagtaaaaca
     2461 tgttctgaac taactgattg atgtttcaac cgttttgctt cttcttgtgc aatttctaat
     2521 actgcttggg ctctttcagt aaaaagttct tccatctaat ctcacttccc atcttcataa
     2581 cttaaacgtt ccagaaatgc tcggagaatc cgagcacgtg tatgatcttc atattcattg
     2641 aaattcaatg tgttttttgc aattgcactc aacatcaagt ttccttcttt ttttgtaatt
     2701 atttggtctt cataaagttt ttgaataata gcaaaagcat ttttctcact gattgtatca
     2761 tcaaataact gatttatctg ctcaagtaat tgttttttat ctgaaatcct tacttttgca
     2821 attcgtatat aaccaccacc accacgtttg ctttctacta gataaccacg ttggatggta
     2881 aaacgggtat taatcacata gttgatttgt gaaggaacac agttaaagag gtctgccatt
     2941 tcaatccttc gaatctcgat catttcacta ttttccagaa ttttctttat atatgattca
     3001 attaaatccg aggtattctg atgtgccatc ttattcattc cctttgacta atattgacct
     3061 taattataac gaatttgata atagaaagaa agaagaacgc tttttgttaa ataaatcctt
     3121 tagaatcaac aaaaaaaaat acggaatata ctattccgta tcgacgcgcc caagaggagt
     3181 cgaaccccta accttttgat ccgtagtcaa acactctatc cagttgagct atgggcgcat
     3241 attcaattta aaaaaattat aaatcatttg aaaacaaaaa gcaagataaa aaaaatcggg
     3301 aagacaggat tcgaacctgc gaccccttgg tcccaaacca agtgctctac caagctgagc
     3361 tacttcccgt ttaaaaaata aaaaagac
//
LOCUS       DAZBTF010000155         3380 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000155 DAZBTF010000000
VERSION     DAZBTF010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.163"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..1171
                     /locus_tag="ACHGNS_002924"
     CDS             131..1171
                     /locus_tag="ACHGNS_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFN6938780.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            1995..2327
                     /gene="traE"
                     /locus_tag="ACHGNS_002925"
     CDS             1995..2327
                     /gene="traE"
                     /locus_tag="ACHGNS_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFN6938781.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            2946..3287
                     /locus_tag="ACHGNS_002926"
     CDS             2946..3287
                     /locus_tag="ACHGNS_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFN6938782.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1257 a          458 c          643 g         1022 t
ORIGIN      
        1 ttctgatttt aggtagtatt taattaaaaa acagttttaa tgcgtttgca ttttttattt
       61 atctttgcta ttttcgaaaa gagtaggtaa ttttacataa ataaaacaac gtgaaagtag
      121 aggatctaca atgactgatt ttaaattttt taaggcggat agagtttaca acgaattatt
      181 ttatcaattt ccaaaagtct ttattgtttc tgacgaatac aaaaaaatga aagattcaac
      241 taagattgcc tatatgcttt taaaagcaag attagagatc gcaatcagca aacggcaaat
      301 cgatgaagaa ggtaatgttt attttactta tacgacaaat gaactatgta gagtattaaa
      361 ctgccaaaaa caaaaagcga tagcaatcaa aaaagagttg gaatcctttg gtttattatt
      421 acaaaagcag atgggattta acaaacagtt agggaaaaat aatcctaata gactatatct
      481 agcagaatta aaagtctcag aaaatgatat ctacttactc gaaaaatttg atagagagaa
      541 tagggaaaac gttgataaat cagagggtat gaaaatcata cccaccctcg acgaaaaatc
      601 agacgctgaa tcccttgggg ctcaagaggg tatgaaaatc ataccgtgcc aaaacgttga
      661 taaatcagag ggtatgaaaa tcataccaga acttaataat aatatattag acactaatag
      721 acacaatata gacactgaaa aagaccgcct acaagatcaa ttgttgttag acaattttga
      781 gacaattatg acaaacgaca gcattgctac gtttgtccct gaacgagtat taaatttgat
      841 aaaaacattt tcttcaagtt acagtgaagc tcaaaaaacc gtccagacta ttcataatgc
      901 aaagaaaaaa gctgaaatag aaagtgatat ttcgatagtt tttgaagaac tcgatagtta
      961 ttatgtcaat gcagaacaag aattatacac gacactgtta aaagcctatc aaaaattaaa
     1021 aaccgaaaaa gtcgaaaata tccagaacct gatttttgtc tatgtaaaaa attggtttat
     1081 cgaaaaacca atagctgcta aagtatcaag tgaaaaacgt ttgaattatg caagctcccc
     1141 aagcactatt acgaaagact ggttagagtg aaactttact gacaaagtga ttgttgcttc
     1201 atggagtaac attaaaatta aaaatttttt attattctaa ttatattacg aatattacaa
     1261 aataatagct attttaatag aatttataag ttaaaatatt atgataaaaa tcacaaacgt
     1321 agccttttac gtcataagtg ttaaaagaaa taattaattt caaatataga aaagggaacg
     1381 tatgtttgca tactcctttt ttttgagtta taataatacg cgaagagaga taatttcatt
     1441 atttaaacaa gctaaaaatc ttgatattac ttcttttcag gcaatacgaa tattattcgc
     1501 ttacattttt tgtataaatt taattgtctg ctatgcttgt gatgaattga ttttcgtttt
     1561 gcacttttgg atgataaatt tggacaattg aaaatacaaa aaaaagaaag gcggtatgaa
     1621 ctcggaaggc tggtaacctt cgtcagcgtt catacacggc attaagattt ggcccttaat
     1681 gcgttaacct ttcagtgtta cggtattacc cgcaatggat ttagtataac agatattcaa
     1741 aagtgatacc actttttttg gctgtgttaa ggttaaaaat acgtgatatt acgatgaatc
     1801 tatgcctaag cataatgtta taaaaatgtc cgtgtggctc tttgccgtat gacgaacgct
     1861 gaaattagag ggaacgcatg tttggatgcg ttccctcttt tttgtttgtc atgaacgaaa
     1921 atcaattctc tatttttgaa aaataacaag aaaaacttgt taaaaatatt taaagaaatt
     1981 gggtggttta ttttatgtca tatacaggaa ttttgagtct caaagatatt tgtcactacg
     2041 gtaaacggtg taccgctaca gagaaaatca cgaagaaact atcaactggc caaagtaaaa
     2101 ccgttgtgca gtgtaagaaa tatataatcc agaaagacaa agtctcagag gaaatgattt
     2161 attacattgg gaaacaaaag caaataattt taaaagatcc tatcccctta aaagagctat
     2221 atccaacaat caaacatgtt tatgaccaaa acggtgtact gattggtcga agaaaaaatg
     2281 gagtcttgcg ttgtaccgca aaagggatgg ggcgtttgat cggctgaatt gatcattctc
     2341 ataacttgta ttttatcgtt tttcgcttgc ttgaaaatat tgtcgagact tctaaaggaa
     2401 cttagagtca ctagaaaaat aaggagtaga cttgatctat caacaattcc cttcggggag
     2461 tgacggggta ggttttaacg attggtaacg taaaaaattg gtcaatcgag taaatgcgct
     2521 tgtttggata gtggtgaaat gaatttgtgt aaataataag cgcagagtaa acaagaggga
     2581 atttagaagt tagacaagtt agaatagcgt ctagattaat catgagcagc tactaaggga
     2641 tctagacttt gacgcaaact catggaataa ggagtaggtc ttctcgtaat ggggaggacg
     2701 cacaagaaat tgtgtcgtgt gctgcagtac atgtagatac ttgcgaggac gttgattatt
     2761 ggatcaatcg agcatttacc gctgtctact agggtttctc gtttcggacg ccataaacga
     2821 gtcgaaattt tttatgtatt tttaagtaaa cgaaaaacga taaaatatga acaagtcctg
     2881 aataaagtga aaaaattgat ataaaacata gagaaaatga aatttacgta atggaggaat
     2941 gacaaatgaa aaattatgat ccgaatatac gattgggcac acatactatt aaggtttctt
     3001 tccaacgttg ggattacaaa ggatttgtga cgtttagaag gggcggaaat tgtaaaggat
     3061 tagatgtttt agcattagac gaggatgatt tatacgatca aacattgact gataatccaa
     3121 ttggattcgg cttgctacct gaagatgatg aaggtaatga atggtttaaa atgacgctta
     3181 tgaatgataa tggagacgaa ttatctgttg aagatacttg gtcatatttg agtgattata
     3241 ttgtcagctt tgaaatcatt gaatttgtag cggataaaga agaataaaaa ctggaggaat
     3301 aaattttaca gataaaggct tagaaggagt cacgacgatt aataaaaagg tagtattacc
     3361 ttaggcattg agtaaggaat
//
LOCUS       DAZBTF010000156         3071 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000156 DAZBTF010000000
VERSION     DAZBTF010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.164"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            185..418
                     /locus_tag="ACHGNS_002927"
                     /pseudo
     CDS             185..418
                     /locus_tag="ACHGNS_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(466..585)
                     /locus_tag="ACHGNS_002928"
                     /pseudo
     CDS             complement(466..585)
                     /locus_tag="ACHGNS_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
     gene            complement(1801..2967)
                     /locus_tag="ACHGNS_002929"
     CDS             complement(1801..2967)
                     /locus_tag="ACHGNS_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFN6938783.1"
                     /translation="MKISVFGLGYVGLANSLLLAQKEQVKAYDIVEEKITMLQNGISP
                     IEDKEVHEFLKRDDLNVEFTSNFTDAVNFGDYLIIATPTDYDEKKNYFNTSTVEDVIE
                     KALAIRPDSTFIIKSTVPVGYANEIKAKFGTENIIFSPEFLREGKALYDNLYPSRIIV
                     GECSARGREIATMFQRNAFNKDASVLLTHSTEAEAIKLFSNTYLALRVAYFNELDTYA
                     EFRGLDSKQIIEGVGLDPRIGSHYNNPSFGYGGYCLPKDTKQLRANYEKVPSNIISAI
                     VDANTTRKDFIAGQILAKKPNVVGIYRLTMKAGSDNFRYSSIQGIMKRIKAKGIEVVV
                     YEPTLDEDEFYHSQVLKDINEFKNISDVIVSNRFEDILKDVEEKVYTRDIFGDN"
BASE COUNT         1034 a          584 c          418 g         1035 t
ORIGIN      
        1 atatcaatat ccatttcgag gaaaattgtt taactaaaga aaaatacaaa ggacaaattt
       61 gcttgattta caaagggact cttcagtaca caccagaaga atatccacat cttccaatat
      121 attaaaagaa ccttcttgaa tcattgagtg aaagaaaaga gattcgtcac tatttgagga
      181 ctgtttaact agagacatta gcgatctgcc tgaaacctat acaaccactt tacgaacgtt
      241 taaaaatttt ctgccacaga ttcaaaatgc gcttaactgc agctattcaa acagaccatt
      301 ggaatgttta aacaaccaca tcaaagtatt aaaacgcaat acgtatggct ttcgcagctt
      361 ttacaatttc aaactacgga tcatgattcg tcatggcaaa acatttttaa ccaaataaac
      421 agaagaatgt gggataaatc taaaaatcag ttttgctaca gactctcgag taaacggcgg
      481 gagcagaagc aactccttcg gaaataagcc gaaattcaca aaaatttgaa aagcaatttt
      541 cgtgaatttc ttcttatttc tcggagttaa acacttctgt cccagcctct tctgtccaaa
      601 ttattctaag atttctcttt accaacatca tttgacaaag agcttctttt ttgaatacta
      661 tattcgttct gtttttcatc ttcaaatacg aactcatccg cagaaatatt tgtcacaact
      721 ccatgaatct tcttttcatt accacctata taccacagga ttcctacaca gaaacaacaa
      781 aaaaatagca taaaataggt tttctttatt ttatatccct ccttttagat taaaagctca
      841 cagaaaacgc tttctaaaaa gacaatagaa taatcataat ttttcatttt gtattattta
      901 tacaaataat acgcatgtta ctgaaaaatg atatggttcc ctactttttg agataagtta
      961 aataaaatct gtcatcgata aaacagctag tacagtaatg ataaatagta agaacttaaa
     1021 atattttgga tttttatttt tgaaataatc gaagaaaagt aaaagtgata acgaaatagc
     1081 aaacaagatt atatctataa gattattcat tgagaaatcg ccccatatgg aactctattt
     1141 tgacctgaca caccgagaca taaagcaatc atatcctctg cattgtttta tactattaga
     1201 actatagcta cacctattgg atgctactgg ctctaagatt aatgctaata cagtaacatc
     1261 aatacctaaa ctagcagaaa ctctatttgc atcttcaatt tcagatgcct ggactgtcgt
     1321 gactagcgcc gtagtagaaa aatttactgg tactaacaaa gatgcagaca cgacttttat
     1381 ttcacttatc ctttatccca taacctaaac ttcaatcatt acttgtgagc tgatcaatgt
     1441 tcttccaagt ctgaaagaac attgatcagc ttggcaaaca aataatgttt tcaaatactt
     1501 gatcgatgaa aaaaagctat tggtaataac agtaattgca ttttccattt tcgtgtcaga
     1561 tacttcgctt acagctggta aacgatcaat cccattgtca aattttggat ttttttctga
     1621 tgatgtcttg ctttagttca taaaataatc cagcaataaa ttcctcgcaa ggatagccaa
     1681 cttcattttc ctaatcggaa tttttaaact tatcggtata gatacagatg caatgagaac
     1741 aaacatttat ttcgagtcac catctaaaat attttcctcc atgtacccgt tcattattat
     1801 ttagttatca ccaaagatat ctctggtata aactttttct tcaacatcct ttaaaatatc
     1861 ttcaaaccga ttagatacga tcacatcaga aatattttta aattcattaa tatctttcaa
     1921 tacttgtgaa tgataaaatt catcttcatc caatgtaggc tcgtaaacaa ccacttcaat
     1981 tcctttggct ttaatccgtt tcataattcc ttggatcgac gaatatcgaa aattatctga
     2041 accggccttc atcgttaaac gatagattcc aacaacattt ggttttttcg ccaaaatctg
     2101 tccagcaata aaatctttcc ttgttgtatt tgcatcaaca attgcactga tgatattgct
     2161 tggtactttt tcatagttgg ctcttaattg ttttgtgtct ttgggtaaac agtatccacc
     2221 atagccaaaa gaaggattat tataatgcga accgatccgt ggatccaaac cgacaccttc
     2281 aataatttgc ttactatcaa gcccacgaaa ttctgcatac gtatctaatt cattgaaata
     2341 cgccactcgc aatgccaaat atgtattcga gaataattta atagcttctg cttcagttga
     2401 gtgagtcaaa agaactgatg catctttgtt aaatgcgttt ctttgaaaca tagttgcaat
     2461 ctcgcgacca cgagcagaac attctccaac tataattctt gatggatata agttgtcgta
     2521 caaagctttt ccttcacgca aaaattcagg tgaaaaaatg atgttttctg taccaaattt
     2581 cgctttaatt tcatttgcat agccaactgg aactgtcgac ttaataatga atgttgaatc
     2641 tggacgaatt gctaaagcct tctcaattac atcttctact gttgatgtat taaaatagtt
     2701 tttcttttcg tcataatctg ttggagttgc aataatcaag taatcaccga agtttactgc
     2761 atcagtaaaa tttgacgtaa attcgacatt taagtcatcc cgttttaaaa attcatgaac
     2821 ttctttatct tctattggtg agatgccatt ttgtaacatc gtgatttttt cttccacgat
     2881 atcataagct ttgacctgct ctttttgtgc taataatagt gaatttgcta gaccaacata
     2941 gcctaatccg aaaactgaaa ttttcatatt cttccatacg ctccaatcaa atttcatttt
     3001 atagtatttt agctgttatc cttttgaaca tattccttat tcaatcaata ttatttaata
     3061 gaaaaaattt t
//
LOCUS       DAZBTF010000157         2943 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000157 DAZBTF010000000
VERSION     DAZBTF010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.165"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..687
                     /locus_tag="ACHGNS_002930"
     CDS             <1..687
                     /locus_tag="ACHGNS_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFN6938784.1"
                     /translation="LNILEACRNFPVKHLLYASSSSVYGGNTKVPFSTEDSVDHPVSL
                     YAATKKSNELMAHTYSHLYKIPTTGLRFFTVYGPFGRPDMAYFSFTKNILSGKEIKIF
                     NHGKMERDFTYIDDIVEGITRLINKVPEPNSDWTDIDDLSSESWAPYKIYNIGNNKPV
                     ALLEFVETIEKYLGVKANKIFLDMQPGDVVRTYADIEDLEEKINFKPNITLDEGIKKF
                     VEWYTVYYLS"
     gene            1055..2479
                     /locus_tag="ACHGNS_002931"
     CDS             1055..2479
                     /locus_tag="ACHGNS_002931"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFN6938785.1"
                     /translation="MDSSSNKSHSIAKKAFIYAIGTLSSKMISVIIIPIYAVYISVAD
                     LGSFDYQNTIAQFLSPVIFGAIWEAILRMGLGKEKNIQKKVVTNSICFVIANTVILAI
                     FLPIIYRMIFPNLEYYQLFVFQLIFTPILSVFQYIVRALGESKIFVMSGIIAAGINLL
                     LLTIFVVTMGMGILGLLLSIIISNVINLIFLIFRGQIFQYFATSSFDFLFLKSMLKYS
                     IPLTLNLTFGWLIVGFSRFYIGTYIGVTENGIFSFAMKFSSIILTLTSIVSMSMIEDA
                     VITSENDDFIERFVEKNTYIFKMYIRLATLSIPIICCAYSFITNPDYKKSLVLVPILV
                     IMACFSGMTTNIGNIFAVYNKTNQLLMISVVSGLLSVVGTYLLGYSFGVIGVSFATLL
                     GTLVLFGLRYYFGRKIAYYAFDWKRISLLLVLYSAIAFVCIHRGFYFNLFLTIIFSAI
                     FVFTYKQLIITYLKKFFRIKMAKR"
     gene            2565..>2943
                     /locus_tag="ACHGNS_002932"
     CDS             2565..>2943
                     /locus_tag="ACHGNS_002932"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938786.1"
                     /translation="MIFGKKHSLVEVPEFGSEPYLIEIGDNFRCSFGVKFITHDGGVW
                     VLRKMSSVSESTSKIGRIKLGNNIFIGIYSIVFPGVSIGDNVIIRANTLVNKDIPNNS
                     VVAGSSCKVISTIEEHYEKNKNEF"
BASE COUNT          961 a          394 c          542 g         1046 t
ORIGIN      
        1 cttaatattc ttgaagcctg ccgaaacttc cctgtaaagc atctattata tgcatcatct
       61 agttcagtat atggtgggaa cacgaaagta ccgttttcaa cagaagattc ggtggatcat
      121 ccagttagcc tttatgcggc taccaaaaaa tcgaatgaat taatggctca tacttatagc
      181 cacctttata aaataccaac aactgggctg agatttttca ctgtctatgg tccatttggg
      241 cgaccagata tggcctattt ctcttttacc aagaatattt tatctggaaa agaaattaag
      301 atttttaacc atggaaagat ggaaagagat ttcacttaca tcgatgatat cgtagaggga
      361 atcactagat taatcaataa agtacctgaa cccaactccg actggacaga catcgatgat
      421 ttgagtagtg aaagttgggc accatataag atatacaata ttggaaataa taagccggtt
      481 gcattgttgg agtttgttga aacaattgaa aagtatttag gtgttaaagc gaacaagatt
      541 tttttggaca tgcagccggg tgatgtcgtt agaacttatg ccgatattga agatttagag
      601 gaaaaaatca actttaaacc gaatataacc ttggatgagg gcattaaaaa attcgttgaa
      661 tggtatacag tgtattattt gagttaattt tgaaaggaat attgaagatg ggtaattcat
      721 acgatttttt tagctttgtt aggtatttta ggaaattcta ctttcaaacg tgacgaactt
      781 tctcgccgaa tagatatttt ctataactaa aagaatttct ataaaacaaa attgtgtatc
      841 aagatgtgtg aactggattc tgactgccaa tattgattga tgagattgaa gaaatatata
      901 gttgatcatg gatatcttta tttgaaaaga tcttagctat tcacatattc aaaaaaattt
      961 ttactacgag gactcacacg atacagagga atttaggtag ttctaattaa accagaaagt
     1021 catctataga tgtgagttga gggaggtcat acggttggat agtagcagta ataaatcaca
     1081 ttcaatagct aaaaaagctt ttatttacgc aataggaaca ttatcatcaa aaatgatatc
     1141 tgtcattata atccctatct atgcagtata tatttctgtt gccgatttag gaagctttga
     1201 ttatcaaaat acaattgctc aatttctttc tccagttatt tttggagcaa tatgggaagc
     1261 aattttacgc atgggattag gcaaagaaaa aaatattcag aaaaaagtag tgacgaacag
     1321 tatttgtttt gttatagcaa atacagtgat attagccatt tttttaccaa taatatatag
     1381 aatgattttc ccgaatttag agtattatca attgtttgtt tttcaattaa ttttcactcc
     1441 tatcctaagt gtttttcaat atattgtaag agcactaggt gaatcaaaga tttttgtgat
     1501 gagtggtata atcgctgcgg gtatcaacct gttactcctt actatttttg ttgttacaat
     1561 gggtatgggg atacttggtc tcttactctc tatcataatt tctaatgtta taaatttgat
     1621 ttttttgatt ttcagggggc agatctttca atatttcgca acgagttctt ttgatttttt
     1681 atttttaaag tccatgctga aatactctat cccactcact ttgaatctca cttttggatg
     1741 gctaatagtt ggtttttcaa gattttatat tggaacgtac atcggagtaa cagaaaatgg
     1801 tattttttct tttgcgatga aattttcatc tataatttta actcttacaa gtattgttag
     1861 tatgtctatg atagaggatg cggttatcac gtctgagaat gatgatttca tcgaaagatt
     1921 cgttgagaaa aatacctata tttttaaaat gtacattagg ttagcgactc tttctattcc
     1981 tatcatttgc tgtgcatact cttttataac caaccctgat tataaaaaat cattagttct
     2041 agttccaata ttagttatta tggcatgttt cagtgggatg acaacaaata tcggaaatat
     2101 atttgcagta tacaataaaa caaatcagtt attaatgatt tcagtagtgt caggtctatt
     2161 gagtgttgtt ggcacctatt tattaggcta ttcttttgga gtaattggtg tctcatttgc
     2221 tactttgctc ggtactttag tattatttgg acttagatat tattttggaa ggaaaattgc
     2281 ttattatgca tttgattgga agcgaatatc gctactttta gttctctatt ccgcaatcgc
     2341 ttttgtatgc attcatagag gcttctattt taatcttttc ttaacgatta tttttagtgc
     2401 tattttcgtt tttacgtata aacaattaat tataacgtat ctaaaaaaat ttttcagaat
     2461 aaaaatggct aaaaggtgag ggtatacgaa gtaagaatag aatctttcga agaataaaga
     2521 gaaaactatt tggaaaaaat ctgcgagaat gtattaatga aggtttgata ttcggaaaaa
     2581 aacatagttt agtagaggtt cctgaatttg ggagcgagcc atatttaatt gaaatagggg
     2641 ataatttcag atgttcattt ggtgttaaat ttataacaca cgatggtgga gtctgggtct
     2701 taaggaagat gagttctgtg agcgagtcaa cttcaaaaat aggtaggatc aaattgggaa
     2761 ataatatttt tattggtatt tactctattg ttttcccagg agtttcaatt ggcgataatg
     2821 taattattag agcgaacact ttagtaaata aagatatccc aaataatagt gttgtagcgg
     2881 gtagttcatg taaagtgatt tcaactatag aggagcatta tgagaaaaat aaaaatgaat
     2941 tta
//
LOCUS       DAZBTF010000158         2766 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000158 DAZBTF010000000
VERSION     DAZBTF010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.166"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            107..553
                     /locus_tag="ACHGNS_002933"
     CDS             107..553
                     /locus_tag="ACHGNS_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFN6938787.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            826..2178
                     /locus_tag="ACHGNS_002934"
     CDS             826..2178
                     /locus_tag="ACHGNS_002934"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFN6938788.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            2349..2636
                     /locus_tag="ACHGNS_002935"
     CDS             2349..2636
                     /locus_tag="ACHGNS_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFN6938789.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT          974 a          451 c          545 g          796 t
ORIGIN      
        1 ttttttcatt aaaatacaat aaaattgaat tttcttttca tgttaagtgg attttaaaat
       61 gtaaaaatag tatgataaga cgagaataac aacgtggggt gtttacatgg aaaataagtt
      121 agtcgaagag gttatcgaaa agttaaaaaa agagaacatt cgaatcacac ctcaacggta
      181 cgcggtttta gaatatttga ttgaacatca ttcccatcca acggctgatg agatttataa
      241 agcacttgaa catcggtttc caaatatgag tgttgcgact gtttacaata accttcgctt
      301 atttacttcg attggatttg ttcaagaaat gaagtacggc gatgcttcta gccgatttga
      361 tttcagttca aagcgtcatt atcatgtaat ttgtgaaaac tgcggtaaga ttgttgattt
      421 tcattaccca gggcttgaag atatagaaat ggctgcaagc agactgactg attttgagat
      481 taatgaacat cggttggaat tgtatggact ctgtccggat tgtaaagagt cagcaaaaaa
      541 ggcgcaaatg taaaaacagt ttctacaagt gttataccta aaaaaagcta gctgtgtaaa
      601 aaaaacgcta gcttttttat ttttttgtct tttattcaca atcttagata taaaagtgaa
      661 tattattaaa ataagaatct tgtagatatt gggttcttat gcttgaaaag gaatgaaact
      721 acaaacaaac ctttgtgaat tcacaagcat taaactgttg tatccgtttt atttctgtta
      781 tagtagtttt gtcgaaggac aaaatgttaa tcggagggac cataaatgaa agtagtagta
      841 attggctgta ctcatgcagg tactgcagct gtaaaaagta ttttaaaaaa tcatccagaa
      901 gcagaagtga cagtatatga aagaaatgac aatatttctt tcttatcttg cggtatcgca
      961 ctttatgtcg gcggagtagt aaaggaccca gcaggcttgt tctactcaaa tcctgaagaa
     1021 ttatcttcat taggagcaac agttaaaatg gaacacgatg taacagacgt cgatacagaa
     1081 aataaaacag taacggcaaa agatctgaaa acaggagaaa cagaaacggt ttcttatgat
     1141 aaattagtga tgacaactgg ttcatggcct attatcccac cgattagtgg aatcgatgcg
     1201 aaaaatgtac tcctatgcaa aaactacaat caagcaaatg aaattattgc acaagcgaaa
     1261 gaagcaaaac gtgtagtaat tgttggtggt ggatatatcg gtattgaatt agtagaagct
     1321 tttgtcgaat ctggaaaaca agtgacattg attgatggat tagatcgcat cttgaataaa
     1381 tatctagata aaccattcac agatatctta gaaaaagaat tgacagaccg cggtgttact
     1441 cttgcgctag gcgaaaatgt tactgaattt atcacagatg aagaaggaaa agtgaaacaa
     1501 gtagctacac caacagatac atttgataca gatatggtca ttttatgtgt gggcttccgt
     1561 ccaaatacaa aactgatcga aggaaaagta gatacactgc caaacggggc aatcaaagtg
     1621 aatgaataca tgcagacaag taatccagac atttttgcag ctggagactc tgcagtagtc
     1681 aactacaatc caagtggcac gcaaaactat attcctttgg caacaaatgc cgttcgtcaa
     1741 ggtttgcttg tgggaaataa cttgacagat cataaaatgg cgtatcgtgg tactcaagga
     1801 acttcaggac tttacctatt tggctggacg atcggttcga caggtgtgac caaagaaagc
     1861 gcaactttga acgatctaga agtccaagcg actgtatttg aagataatta tcgtccagag
     1921 ttcatgccaa ctactgaaaa agtaatgatg gagctagtct atgaaaaagg aacgaatcgt
     1981 atcgtaggtg ctcagttcat gtccaaatac gacattacac aatcagcaaa taccatgtct
     2041 ttagctgttc aaaataaaat gactgtagaa gatctcgcac tttcagattt cttcttccaa
     2101 ccacattttg accgcccatg gaattacttg aacctacttg cacaagcagc gctaggcgaa
     2161 atcgaaactg cacaataaag aaaaagggtt gtgacacaat tttgtcacga ccctctgtct
     2221 ttttttgtga aaagtacaat tagttgtctt tcttcgaatt ttttgtcaca ataagcagca
     2281 aagataaagc tgttacataa aagaacaagc agctttattt gattttgaaa agaaaggtga
     2341 gaaagaagat gaataaagaa tattttgtcc aacaagatgc atttttagaa gattataaaa
     2401 gtgcttcgaa gaaattaacg atcaatattg atgagttggc agatcaagta ctctctcagt
     2461 tgaatgaaac gcaagaggaa tattacacga tgccagcaaa tgacgccaaa gataatgttt
     2521 ctcatcgttt tcctttcagt agacgcatct actcagatga aaatgacggc acattgaata
     2581 cagagtacac atatgaaggt cagccatatg tgctagaaac tgatgatgat ctttaaagac
     2641 agtttttgaa aaggccgctc atccttaagt gatgacaaaa aatcatcaca attaccgatg
     2701 actgtctctt tactatcttt taatcagaaa aaggggttat gacaagcttt gtcaacccct
     2761 tttttt
//
LOCUS       DAZBTF010000159         2672 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000159 DAZBTF010000000
VERSION     DAZBTF010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.167"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            699..785
                     /locus_tag="ACHGNS_002936"
     CDS             699..785
                     /locus_tag="ACHGNS_002936"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFN6938790.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(1087..1311)
                     /locus_tag="ACHGNS_002937"
     CDS             complement(1087..1311)
                     /locus_tag="ACHGNS_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938791.1"
                     /translation="MEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNMTPERKRS
                     LEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            1677..1961
                     /locus_tag="ACHGNS_002938"
     CDS             1677..1961
                     /locus_tag="ACHGNS_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938792.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            1964..2380
                     /locus_tag="ACHGNS_002939"
     CDS             1964..2380
                     /locus_tag="ACHGNS_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938793.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(2517..>2672)
                     /locus_tag="ACHGNS_002940"
     CDS             complement(2517..>2672)
                     /locus_tag="ACHGNS_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFN6938794.1"
                     /translation="PALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSSLTSDLPKIS
                     LRNWLEE"
BASE COUNT          881 a          497 c          430 g          864 t
ORIGIN      
        1 cgttttttca tccattcctt tcttcccccc ttacaaataa ataataacct ttaacaacca
       61 actcgtaaat aggttggttg ttattataca aaaggatatt agtactcaga taaaaagttg
      121 ttagtactca gataaaaagt tgttagtact cagataaaaa gttgttagta ctcagataaa
      181 aagttgttag tactcagata aaaagttgtt agtacccttc tgtatccgtt gggagagtaa
      241 ggctcaactg ccccctaaaa agggttctaa aaaatcttta aaaaataaat aaaataaacg
      301 cgcgcgcgcg agcaaaattt cccgccaaaa aatcacccac tttttaaaat aaacaggttc
      361 gcgcatgata ttaaactgcc aatctagctc cctatggtcg ctgtaagaag gagtgatcgc
      421 tacgctctca cattttactt gcagcagtcg cctttaggct ccttcgccaa aaataattca
      481 ttagggactc ctttgtggcg tcctgagagc tcgtctaagc cgttctaaga caagattcga
      541 taaaaactat gtccttcgtt ctaaagcgcc ttacaggcgg tcagggcaag ccctaacaac
      601 cccaaggcaa taaaaaactt aggcttattt ttgctttttt attgtataat tgattgtcgc
      661 ctatccgaca gtcttgcgac tggaaggagg tgtgtttcgt gttcactaac tttatttgct
      721 cccttcttgt tgggctttta agcgctttgt tcagttactg gttgaataac cgaaacaaga
      781 agtaaaactt aattaaggcg actcagccca caaaaaaagc cccagagaat ccgacctctc
      841 tggggctttc gtgtgtttcg tgttcactta tttgcaatca aattataaca gaaataaata
      901 tttatttcaa aaatatccta caaaagtcta aaatccgtaa ttatttctta tatgcaacat
      961 ttacaaataa agtcgctctg agagctcgta tttttcgatt taagacgttt tatttagttt
     1021 ttggtgtatt tcattggtcc aagctaaaaa actcttagaa gcgattttag agccatttaa
     1081 acccacttaa ttatattctc catgaaattc gctccatgcg tcaaaagctg cgtctgaaat
     1141 tccgccgtct tcgttcacta gttgcattaa tttttccaac gatcgttttc tttcgggtgt
     1201 catattttta tattccaaca atttttcgtc tgttaaatcg aacttgattg cttgaattgc
     1261 ttgctctaat ttttctaagt tttgaaaata ttcgtaagga aatttttcca ttttttcttt
     1321 ctccagtctc acaatttttc tagaaataaa atcacttctt tgccgtctat actctcaaaa
     1381 aaaatgtcgt ttgaaaaact aaataaattt ttgatcgtgc atttttaaat ttggttatcg
     1441 attacacgtg cagcactccc ccccgaattt taaagagcat aaggaaacgt gtacccactt
     1501 tctaaaaaaa gtgggtagat aggctgtgcc tatcacgttt ctcagaaaaa aatttgatat
     1561 cactttttcg ccatcgtggg tagcttatgt atagcatttt ggcaaaaaac agctatacaa
     1621 atatagtatt tgtatggtat acataaatta tacataattg caaaaggaga ttttttatgg
     1681 acattttagt tcggaatatt gatccaaagt ttgttgcaac aatagacaaa cgttgccaag
     1741 aactcacgaa taaaacagga gaaaaatgga gtcgaaataa atatctaaaa gttttaatcg
     1801 aaagtgattt tgaccgtcaa cttacgactt acaaaaaaga ccaattcgat ttattgctcg
     1861 ataaatttat tgctatccaa acagaaaata ctcaaatatt aagcgagtat gtctacacaa
     1921 ataatcaaat cattgaaatg cttatcggag gaatgatata accatgagaa aaacttctgt
     1981 ccaaaaacat gttactttgt accctagtca tgttgacgtt ttagattcca tcatcgaaaa
     2041 aaatccagat atcaataact actcggaagc cattcgcttt ttatgtatga gttatgaacc
     2101 cgataccaac acgaccacta ccagcaatca ggcaaaacta aatgctatgg gaaaagaaat
     2161 ttccatcatg actgaaatca tcgcagaatt tgctgattta tatctaaaag atacaggcgt
     2221 tcttattggc acaaattcta aagtatatca agaagtgaaa gaaaaaatcg aagctaaact
     2281 aaatccatca aatcgaaatc gaccaaagaa tcatcaaaaa gaaaccagtc aaacagtcac
     2341 ttctagaaat ttcgacccaa caaattttct aaaacgataa aaaacagata ggagttattc
     2401 ctatctgttt tttatttaga agccccatac aatatgctag aatgtactag caatcttagt
     2461 cggttgtaag ggacttgtct acttgcttaa tgtaaggggg gcgagttctt ttttatttat
     2521 tcttctaacc agttacgcag agaaatcttt ggcaaatctg acgttaggct tgaaaaagta
     2581 aattctaatt tggcgatttt attgcccttt tttgctttta tttttgtgac tttaagatca
     2641 ttgaagtatt tttttagttc tcgtaatgca gg
//
LOCUS       DAZBTF010000160         2613 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000160 DAZBTF010000000
VERSION     DAZBTF010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.168"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(204..494)
                     /locus_tag="ACHGNS_002941"
     CDS             complement(204..494)
                     /locus_tag="ACHGNS_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFN6938795.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(630..809)
                     /locus_tag="ACHGNS_002942"
     CDS             complement(630..809)
                     /locus_tag="ACHGNS_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938796.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(806..1156)
                     /locus_tag="ACHGNS_002943"
     CDS             complement(806..1156)
                     /locus_tag="ACHGNS_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938797.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(1149..2474)
                     /locus_tag="ACHGNS_002944"
     CDS             complement(1149..2474)
                     /locus_tag="ACHGNS_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFN6938798.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
BASE COUNT          770 a          514 c          395 g          934 t
ORIGIN      
        1 ataatcactt tttattactt taaaattttt tattaatgat aagaaatatg ttttgcgagc
       61 agcaactaga ataatttaaa cattactaaa ctgtctatat tgttactaat aattcccttc
      121 ttatagtgca ttttcttaga aaaaaacaat attacgatat catgatatta agatatcgta
      181 atattataca attgtgattg atattatata tgttttttta tttttttatc atgtttaatc
      241 ttgaaggaat catattcttt ttctaaagac aggagctttt cataaacatc agacatgttt
      301 aaactagagg ccaggcttaa atcataaacg aatgaagcag gtaatgattt tattctacgt
      361 tgtcgggtta tccagcttgc aaccgttcct tgtgggtagg catataactc acaaaattgt
      421 tctacagtga ttcctaaatt cttgataatg taaacattga tcgggtgcga gtaaacaaat
      481 gtattgatag acatggtgcg taagcctact ttcttttatt attgcgtttg caataatagt
      541 ttaacacacg cattattcta gtatctactc ctatttgtaa aaacaaaaaa ggattcatag
      601 agataactct atagaatccg taaaatacct tatatatata acgggaagat ataaaaccta
      661 aaattatgat aacctgctgc agcttctaca tagaaagaga agcgctcaac accaacctca
      721 tttatcaaac gttggccata accccaaatc tcttctgata gctgatcaaa ggttatatac
      781 tttagctcat attgctggat aatgttcatt ctaatcgact ccatttatta tcctggtaaa
      841 attcgatatg acgtatttcg tcgtagtgaa ttttgtcttt tccaacgtag atccccagct
      901 catcactacc taagatcttg ccaatgatat cttctggata gtatccttca gcgtttcgtt
      961 cttctttttg aatagatact gatctatttt ttatgactgc agcgttcaat acttccgcaa
     1021 tctcttcagt cgtcatttgt tcttttgcta aattttgact ggttcgttgt tcattgtcgg
     1081 catcaatttc ttcagtatga tcagataaat aaaaccctgc ccactttaaa cgtccacgat
     1141 cattgtaatc aatcattttt aattccccca tttccgcccg catgaccacc aacaagtgtg
     1201 cttctagtaa tgcttctagc gccttctaac attgaagaag catgtacaat cgacgtaaaa
     1261 ccatattttt gccgaatctt gtcaataatt ttatctaaat tttcttcgtc aatttgcttt
     1321 tgtggatcac taaataaatc cagctgaagc gaatcggtgt acacaagctt gctataggtt
     1381 attccaacat gccttacttc ttgtccttca taatacttat caaacaagaa aagacaataa
     1441 ttctgcaatt cctttgtatt atctgtgata gggatcttca tctggtgtga aaaccctggt
     1501 ctcgtttcta agatcgacgt gccaatgtgt aaatggacac aacctgtttt acagttatgc
     1561 tgcctaatgc gtatcgctac ttgttccgcc atttctttta ccaccagctc gatttcattt
     1621 cttctagtat aatctcgtgg taacacttgt gaatttccat atgatttttc ctttgtcggt
     1681 tctggtggga ttgctatatc tgttcgatcg atcccattag catggaagta cagctgtaag
     1741 ccaatcacac ctagtctatt cttaatcgta taaggattcc agttagcaag atctctaatg
     1801 ctcataatcc ccatctgatt gagtcttttt ttcattcgag agccaattcc ccaaaagtcc
     1861 gtcatttcag gaatattcca tactttttca ggaacgtttt cgtacgtcca ttccgcaaca
     1921 aatccttcat tatgctttgc ttcgttgtct aaggctagtt tagccaaaag gggattatct
     1981 ccgacaccta ctgtagcaat caatcctagc tcttctttaa tattctcctg gatcatttga
     2041 gccagcttct ttctccgttg acttcgtgtt ccttcagtcg taaaaagatt cagtgatcgg
     2101 gtcactttta agattgattc atcgatcgag taaatcagta gatcttcatc agccacatat
     2161 cttctgaaaa tattatttac ctgcatattt cgcttgatat ataggttcat acgtggtgga
     2221 acaacgtgta gtgttttagg aaatggttgt ggtaagtcac gtggtctact cacatttgta
     2281 ataccatacc gcttttttgc ttcaggagaa gaagctaata tcaatcctga accagtatta
     2341 tcagctcgac tcataacaac aagttctgtt gttaatggat ctaaatttct ttctatacat
     2401 tcgacacttg cgtaaaagct tttcatatcg attaaaaagt aatcatttac tggctctttt
     2461 gaataatcca tcataaataa cactccattc tttcacatta tacaaacata tgttcgatat
     2521 tgtaaagaag aaatagctaa atacataaaa aaagaagcat aggtatgcta tcaccctgct
     2581 tcttcttttg atttgtatga tcaatttatt cta
//
LOCUS       DAZBTF010000161         2590 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000161 DAZBTF010000000
VERSION     DAZBTF010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.169"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            49..1056
                     /locus_tag="ACHGNS_002945"
     CDS             49..1056
                     /locus_tag="ACHGNS_002945"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFN6938799.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1068..2072
                     /gene="neuB"
                     /locus_tag="ACHGNS_002946"
     CDS             1068..2072
                     /gene="neuB"
                     /locus_tag="ACHGNS_002946"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HFN6938800.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2069..>2590
                     /locus_tag="ACHGNS_002947"
     CDS             2069..>2590
                     /locus_tag="ACHGNS_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFN6938801.1"
                     /translation="MKKTLIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAP"
BASE COUNT          947 a          335 c          519 g          789 t
ORIGIN      
        1 attaagaatc gaattataga tgactataat tcataggagg agatataagt gtttaaagga
       61 aaaacaatat tagtaactgg cggaactggt tccattggaa gtgaaattgt tcgtcagctt
      121 ttaaaatatg aaccaaaagc tatcagaatt tatagtagag gtgaagaaaa acaattttat
      181 atgcaacaag agctctcaaa tcacactaat ttgagatttt taattggtga tatcagagac
      241 tctcaaagga ttgattatgc atgtcgtgat gtggatatta tttttcatgc tgccgcaatg
      301 aaacacgttc cagcaagtga atataatcct atggaagctg taaaaacaaa tgtgatggga
      361 actcaaaatg taatagatgc agcgatcaaa aataatgtaa aacattttgt aggtatcagt
      421 acagataaag tagtgagtcc tactaacacc atgggtgcga caaagttatt gagtgaaaaa
      481 ttaataatag ctgccaatca gtataaagga aaaacagaaa caatattttc ttgtgtaagg
      541 tttggaaatg tcatggggtc aagtggatct gttattcctc tttttagtaa acaaataaaa
      601 aacaataaag atattacttt gacagatgtt gatatgacac gatttatgat gtcaataccg
      661 gatgctgcaa gattagttct gtctgctgct atgaattcaa aatttggaga aacatatgtc
      721 ttaaaaatgc cggcaatcaa tattttaact ttagctcaat cattaataaa aatatataat
      781 gatacacatg atgaaaaata tagaggtgaa ataaaagttg taggtatcag gccaggagaa
      841 aaattatatg aagagctaat gactacagac gaaattgaac gctcatatga aaatgataaa
      901 atgtatatca ttccaccaca ctatcatgct actgacgaac tgtacaaaga ttatgaaaaa
      961 attaaaatca ctgcatactc ttcagatgca acagaattaa tgagtggaca ggaatttatt
     1021 agaatcgcaa aagagaataa tgtcgtgact tggtaaggag ttttatgatg aaatcagaag
     1081 tatatgtaat tgcagaaatt ggtgtaaacc ataatggaag tctagattta gctttagaaa
     1141 gtattgataa agctaaagaa tgtggagcta acgcggttaa atttcaaaca tttaaaacga
     1201 ataagctagt ttccaaaaag gctccaatgg caaaatatca gaaggataat cttaaagagg
     1261 acagttctca atttgaaatg ctaaaaaaat tagaattatc tcaatctgat tttattagaa
     1321 taaaagatta ttgtatacaa aataaaattg attttttgtc tactcctttt gatgaagaaa
     1381 gtgcagactt tctaaaacaa attggtgtac aaggatttaa aattggctca ggagatttaa
     1441 caaatttacc ttttcttaga aaaatagata tgtatgggtt gccagtttta ttatctacag
     1501 gtatggctga tattcatgag gtcagtgagg ctgtagattg tttcatagat agtccagtta
     1561 ctattttaca ttgtacttca aattatccgg ctagttctga agatattaat ttattggcga
     1621 ttaacacatt aaaagaaatg ttcgggatac ccgttgggta ctccgatcat tcattgggat
     1681 atgatgttgg tatttgtgct gttgcgatgg gtgctaaggt aattgagaaa cattttacct
     1741 tggataagga actacctggt ccagatcata aagcttcttt agatcctcag gaatttgcag
     1801 attttgtaaa gcatattaga aacactgaaa tatttttagg tgatggtgtt aagcgagcaa
     1861 tgccttctga acttagtaca aaagaagtat caagaaaaag cctagtggtc actgagaatt
     1921 taaatgtggg agatattctt acagagaaat ctcttacaat aaaacgtcct ggaaatggaa
     1981 tagctcctaa gtttttatat caatatattg gcaaaaaagt taaaagacca ataagtgcag
     2041 atagtgttct gcttgaggaa gatgttgaat gaaaaaaaca cttatagtaa gtggaacacg
     2101 agctgattat ggaatatata aacctgttgc tgagaattta ctacaagata attgggatgt
     2161 atcatttgtt gtttctggaa tgcatttatc ccataaatat ggatttacta aagaattgat
     2221 cttagctgac ggatttagaa ttagtggaga agttcaaagt ttgtttttag agaacaataa
     2281 gggaaacatg gctcggtctg tggcattaga gataaatggt ttcacaaata tatttgaaca
     2341 aactagccct gattttgtat tattactagg ggatagagga gaaatgttgg cagctgcgat
     2401 agcttgtttg tatcttggaa tcaaaacagc acatattcac ggaggcgaag tgtctggtac
     2461 tgtagatgaa tcaataagac atgccataac gaaaatagca tctgttcatt ttgctgctac
     2521 ggaaaaaagc aaagagcgtt taataaaaat gggggaagat tcttggagag tttttcccgt
     2581 cggagctcct
//
LOCUS       DAZBTF010000162         2563 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000162 DAZBTF010000000
VERSION     DAZBTF010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.170"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..809)
                     /locus_tag="ACHGNS_002948"
     CDS             complement(<1..809)
                     /locus_tag="ACHGNS_002948"
                     /inference="COORDINATES: protein motif:HMM:NF026247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsG family protein"
                     /protein_id="HFN6938802.1"
                     /translation="MDTEASLEINQEKSVSQKKWSTSNKILSGVLLLIMWIIMAFNTQ
                     NADYYAYSVLYNGIAQFGMDFSSDSIEPGFRAVMYVCTFISSQYQIFLLIISTVLLSA
                     LLYVIRLFTTNVFLVLFLFFIFGFTSQAIQVRMFSALIFILLAFYHLTKLRGSKAIFK
                     YIFFILVAGLFHNLAYFYLPFFIVKINKKITGYVTILSLLLFSIGQSYISSIFINTKY
                     DAYFDRNSTSISHVVMFALFFSANLFLVHKLSKMNIYEKKVISLSSYIFSI"
     gene            complement(781..1677)
                     /locus_tag="ACHGNS_002949"
     CDS             complement(781..1677)
                     /locus_tag="ACHGNS_002949"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFN6938803.1"
                     /translation="MKNKIGIIIVTYNPDNNIKLLVDTLIQEKQNKLIIVDNGSDNIN
                     SFQFEMMENLILKKMNENIGLATAQNIGIEIAKKEKCDYILLLDQDTIIQKNLSLKLL
                     EEFTNIEAKDKDIGIIVPNYFENSNDESVSFTCKNERGRYVKKHFASTSGSMKIAFAN
                     SSGMFFKSELIDDVGNMKDEFFIDQIDVEFCKRVANNGYNIYVTNQVYFYHHVGDGEN
                     LKIGSKKIKIDNHNSTRKYFIARNSTLVKRMYKSDKEFSRLMNKRLLKFFIAIAFETD
                     KLSRFRAAIRGVRDGYRGVIGD"
     gene            complement(1667..>2563)
                     /locus_tag="ACHGNS_002950"
     CDS             complement(1667..>2563)
                     /locus_tag="ACHGNS_002950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938804.1"
                     /translation="LFIRKEKPEIIYCRIPPNTLVFFIAKFSRCNRVIFDVYDLWPES
                     MPGIGNKYKFFTDLWKKLRDQYLSNSSLVITECDYFKKFIKISGESKNMTIYPCSFNV
                     DDQNDYGEKIVNKSLESTLNLAYLGTINNIVDLEAINELLKTCKRKFGNKINLYFIGK
                     GESKTKLADICEKNHIEFVDCGVIYDNAEKKVILDKCHFGLNLFKSNTIIGLSLKSIE
                     YFRFGLPIISNLSGDTKEIVKRYNVGINYNSTVDENEMLKFYQCNKNINENVRKIYAT
                     IFSEQVLKMKWNEVYKVMNDEK"
BASE COUNT          887 a          429 c          309 g          938 t
ORIGIN      
        1 ttgatagaaa atatatatga gcttaagctg ataacttttt tttcgtaaat attcattttt
       61 gataatttat gaactaaaaa aagatttgct gaaaaaaaga gagcaaacat cactacgtga
      121 gaaatcgatg tactgtttct atcaaaatat gcatcatact tcgtatttat aaatattgaa
      181 ctgatataac tttgtccaat agagaatagg agcaagctaa ggattgtaac atatccagta
      241 atctttttat taatttttac aataaaaaaa gggagataaa aatatgcaag attatggaac
      301 agtccagcca ctaagataaa aaaaatatac ttaaatatag ctttgctgcc tcgcaattta
      361 gttagatgat aaaaagctag taatatgaat atcaatgcgc taaacatacg tacttgtatt
      421 gcttgtgaag taaaaccaaa aataaaaaac aagaacaaaa ctaaaaaaac attagttgtg
      481 aataatctga tgacatacaa taaagctgag agtaagacag tagaaataat caataaaaaa
      541 atctgatact gactggaaat aaaggtacat acgtacataa cagctctgaa tcccggttca
      601 atactatctg aactaaagtc cataccaaat tgtgctatcc cattatacaa gacagaatat
      661 gcataataat cagcgttttg tgtgttaaat gccatgatga tccacattat caaaagtagc
      721 accccagaca aaatcttgtt cgatgtactc catttttttt gtgaaacact cttttcttga
      781 ttaatctcca atgacgcctc tgtatccatc tcgaaccccc cttatagccg ctctaaatct
      841 actgagctta tcagtctcaa aagctattgc aataaaaaat ttcaataatc ttttattcat
      901 aaggcgggaa aattccttat cacttttata cattcttttc accaatgttg aatttcttgc
      961 aataaaatac tttcgtgttg aattatgatt gtctatttta attttttttg atcctatttt
     1021 taaattttct ccatctccaa cgtgatgata aaaatagact tgatttgtta cataaatgtt
     1081 atagccatta tttgcaaccc ttttacaaaa ttcaacatca atctgatcaa taaaaaattc
     1141 atccttcata tttccaacat cgtcaattaa ttcacttttg aaaaacattc cagaggaatt
     1201 tgcaaatgct attttcatgg aaccactagt tgaagcaaag tgttttttta cgtatctccc
     1261 tctctcattt ttgcaggtaa aagagacaga ttcatcattg ctattctcaa agtaattggg
     1321 aacaataatc ccaatgtcct tatctttcgc ttcaatattg gtaaactctt ctaataattt
     1381 taaagaaaga ttcttttgta tgatggtatc ttgatctagt aaaaggatat aatcacattt
     1441 ctcttttttg gcaatttcaa tacctatgtt ttgagctgtc gcaagcccaa tgttctcatt
     1501 catttttttt aaaattaagt tttccatcat ttcaaattga aaagagttaa tattatctga
     1561 gccgttatcc acgataataa gtttgttctg tttctcttgt attaacgtat ccactaataa
     1621 ctttatgtta ttatctggat tgtaggtaac aataattatc ccaattttat ttttcatcgt
     1681 tcatcacctt gtacacttca ttccatttca ttttcaatac ttgctcagag aatatagtgg
     1741 cgtaaatttt tctaacattt tcatttatgt ttttattaca ctgataaaat ttcaacatct
     1801 cattttcatc aacggtacta ttataattga ttccgacatt atatctttta actatttctt
     1861 tcgtatctcc tgataaattt gaaattatag gtaatccaaa cctaaaatac tcaatcgatt
     1921 tcagacttaa accaattatg gtatttgact taaataaatt tagtccaaaa tgacatttat
     1981 cgagtataac ttttttttct gcattgtcat atataacacc gcagtcaaca aattcaatat
     2041 gatttttttc acaaatatca gctaatttag ttttgctttc accttttcca ataaagtaaa
     2101 gatttatttt gtttccaaat tttcttttgc atgttttcaa taattcatta atcgcttcta
     2161 aatctacgat attattaatc gtacctaaat aagcaaggtt caacgtagac tctaagcttt
     2221 tattgactat tttctctcca taatcatttt ggtcatctac attgaaagaa catggataaa
     2281 tggtcatatt ttttgattct ccggaaattt ttatgaattt tttgaaataa tcgcattctg
     2341 ttataactag actagaatta ctgagatatt gatctcgtag ctttttccat aaatcagtaa
     2401 aaaatttata tttgttccct attcccggca ttgactctgg ccataaatca tacacatcaa
     2461 aaataactcg attgcatcta ctaaacttag caataaaaaa aactaaggta ttaggtggta
     2521 ttctacaata aataatttca ggcttttctt ttcgaataaa taa
//
LOCUS       DAZBTF010000163         2502 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000163 DAZBTF010000000
VERSION     DAZBTF010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.171"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..207)
                     /locus_tag="ACHGNS_002951"
     CDS             complement(<1..207)
                     /locus_tag="ACHGNS_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938805.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLV"
     gene            369..1478
                     /gene="pdhA"
                     /locus_tag="ACHGNS_002952"
     CDS             369..1478
                     /gene="pdhA"
                     /locus_tag="ACHGNS_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFN6938806.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            1481..2458
                     /locus_tag="ACHGNS_002953"
     CDS             1481..2458
                     /locus_tag="ACHGNS_002953"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFN6938807.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT          832 a          468 c          526 g          676 t
ORIGIN      
        1 aactaagaaa aaaccattat ttgtacgtcc gttcacgatg tgtaaataaa ataaatgttt
       61 gcaacaaaac tgtataacac caacgttttt aaatagaaac agatggttgt aagtttgatt
      121 agaaaataga aaaaatgcag atgaaatata aaacgctttc cttattgtga ttttattcac
      181 taatgctgtt aatatgcgga ttggcataat ttcctttcat cgtgaaaatt tgtgctttta
      241 gcttgtattt ctcataaatc catgataatt tattaatgaa gacggttaaa tagtgataca
      301 gttttattca ctgtttcaat cgcagaatga agagattttc ttatagatga gagaggaatg
      361 tgtgacaaat ggcaaacaaa aaagcaccga tcgacttcca agcactgcta gatgaagtca
      421 atgcagattt cccagtatat caagcacttg atcaagatgg aaacgtagtt aacaaagatt
      481 tagtacctga tttatccgat gatgaactcg ttgaattaat gactcatatg gtatggtctc
      541 gtgtattaga ccaacgttca acagcgctga accgccaagg acgcctaggc ttttttgcac
      601 caactgctgg acaagaggca agtcagctgg ctagtgctta cgcatttgat aaagaagatg
      661 ttcttcttcc aggttatcgg gatgtacctc aactagtaaa acatggttta cctttatctc
      721 aagctttttt atggtctcga ggacatgctg caggtaattc ttatccggaa gaattgaaag
      781 ctctaccacc acaaattatc atcggtgcac aatatgtaca agcagccggt gttgcattag
      841 gtttgaagaa acgaaacaag aaaaatgtcg tcttcactta tactggtgat ggcggttcat
      901 cacaaggtga tttttacgaa gcaatcaatt tcgctggcgc ttaccatgca aatgccgtat
      961 tttatatcca aaacaacggg tatgcgatct ctacacctcg tgaaaaacaa actgcagcaa
     1021 aaacacttgc acaaaaagct gtcgcagctg ggattcctgg tattcaagtc gatggaatgg
     1081 atccgcttgc agtatatact gtatctaaaa tggcacggga atgggcgatc agtggaaatg
     1141 gaccagtcct aatcgaaaca ctgacttatc gttatggacc acatacacta tctggtgacg
     1201 atccaacccg ctaccgtaca aaagacacag atgacgattg gcagaaaaaa gatccattga
     1261 tccgcttccg taaatatttg acggaaaaag gactttggtc tgaagaaaaa gaagaacaag
     1321 tcatcgaagc aactaaagaa gaaatcaaag cagctattgc tgaagccgac aaagtaccaa
     1381 aacaaaaggt ttcagatttc ttgaaaaaca tgttcgaagt atctccacaa tcaatcaaag
     1441 aacaaattgc aatttatgaa gcgaaggagt cgaaataatc atggcacaaa aaacaatgat
     1501 ccaagcaatc acagatgcct tagcacttga actagaaaat gacgagaatg tcgttgtttt
     1561 tggtgaagat gttgggaaaa acggaggagt tttccgggca actgaaggat tgcaggaaaa
     1621 gttcggagaa gaccgagtat tcgatactcc tttagctgaa tctggaatcg caggattgag
     1681 tttcggtctt gcgttagaag gatttcgtcc agttcctgaa atccaattct ttggttttat
     1741 tttcgaagca atggacgaag tagtagctca aatggctcgt acaagatatc gaatgagcgg
     1801 cactagaaat ctacccatca caattcgttc gccatttggt ggcggtgttc atacaccaga
     1861 attgcattcg gataatttag aaggtttgat cgctcagtct cctggtattc gagtagtgat
     1921 tccttccaac ccttatgatg caaaaggact attgatttct tctattagaa gtaacgatcc
     1981 ggttgttttc ctagaacaca tgaaactata tcgctcattc cgagaagaag tgccggatga
     2041 agcttacgaa gttcctttgg ataaagcagc agttactcgt gagggaacag atgtatctat
     2101 tatcacatac ggagcaatgg tgcgtgaagc aatcaaagca gcagacaatc ttgcaaaaga
     2161 aaatatatct gttgaaatca ttgatcttcg tacagttgcg ccattagatg tagaaacgat
     2221 cattcaatcc gtggaaaaaa ctggtcgcgt agttgttgtc caagaagcac aacgacaagc
     2281 gggcgttgcc gcgcaagtag tttctgagat ctcagaacgc gctatccttt cattagaagc
     2341 cccaatcgga cgagtttctg cacctgatac agtcttccca tttggacaag cagaaaatgt
     2401 ctggttgcca aatgcaaaag atatcgaaga caaagtaaaa gaaatcgcgg aattttaaga
     2461 aaagaaggga cgaaacaaag tcatggctta tcaatttaaa tt
//
LOCUS       DAZBTF010000164         2465 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000164 DAZBTF010000000
VERSION     DAZBTF010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.172"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..566
                     /locus_tag="ACHGNS_002954"
     CDS             <1..566
                     /locus_tag="ACHGNS_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFN6938808.1"
                     /translation="YKHIDVYQKHDYPRINRNNTTAFKIFRAIINRCLRMIKLPPKIR
                     HHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGMFAPDEKFFHTLFFNSSF
                     KNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKWFDERDIDQIKESEKLFV
                     RKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            559..1986
                     /locus_tag="ACHGNS_002955"
     CDS             559..1986
                     /locus_tag="ACHGNS_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFN6938809.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            1988..>2465
                     /locus_tag="ACHGNS_002956"
     CDS             1988..>2465
                     /locus_tag="ACHGNS_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938810.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDY"
BASE COUNT          870 a          288 c          389 g          918 t
ORIGIN      
        1 attataaaca tatagatgta tatcaaaaac atgattatcc tagaattaat aggaataaca
       61 caactgcatt taaaattttt agagctataa ttaatcgttg tctgaggatg attaaacttc
      121 ccccaaaaat tagacaccat aaatttgatt tgtatcatgg ttctcaatgg tgggctttgt
      181 cgaaagaatg cctaacagaa ttaatacaaa tgtatgaaca aaatcaacaa gactacctga
      241 attttaaaat aggtatgttt gccccagatg aaaaattttt tcatacactt ttttttaatt
      301 cctcgttcaa aaataaaaat gttattggag gaccagattt accacttgaa ttgaaaaata
      361 ttgaagaaac tactttacaa acttcgaaat tagcaaatat tcatattatt gatccttcca
      421 tgaacaaatg gtttgatgaa agggatatag atcaaataaa agaaagtgaa aaattatttg
      481 taagaaaagt taaatcggat tactctgata aactattaaa tagaattgat aaagaaattt
      541 tgaaagttgg aaatatagat gaatagaata gtacctgttt tagagaagag tttattaagt
      601 cgagatgcgt ttcgagtaat aaatttaatc atttgtctgc tttattttat gcctagtact
      661 tttaatgtaa ctaatatccc gatgaaaata gcttttgctt ggggtggaat tatagttttg
      721 tatgaggttt ttataaagaa aaattttttt aaaatgcgtt tttcttggct actgattgga
      781 gcaatgatta gttttatctt aactataatc attaacagag aaaatttttt aattccatct
      841 atttataatt ttggatattt gctgattact ttgatagtaa tttttcctgc agacttttca
      901 caaagtgaag aggaaaaaaa gaaaaaaatg gtgtatttta ataatgtttt cattatcata
      961 atttttttag ctgcgctaat atctatctat caatttattt ttttgatttc ttatcatgtc
     1021 ccaacaggaa cgccaggatt acttgcaaga caaggtttta ttgaacatcg gttatttggt
     1081 gtctatacaa gtccaaatgt tggagctatg tttggatata cttctattat tttaagttta
     1141 attagtgtat taatttcaaa aataacaaaa acgattcgtg ttttttatat gatgaatttt
     1201 atcgtacaaa tattttatat tacactttct tcgtctagag gtgcccaggt agtaatttgt
     1261 tcttttttag tattatttct tttgttgttt gccaattcaa aatttagaaa gagcattaga
     1321 gaagtactac ctgtaaaaat caaatggatg gtaagcatat tattagttct attggtatat
     1381 ttttcttttg gtactaatta tgctaaggat tttttagcaa caattcctgt atcgattgat
     1441 agaaaagtag aagttataga gtcaaatgaa tcagataaaa ctccacttcc agaaaaagaa
     1501 gtaatattac agcattctag tgataactcc gaaatttcag ctggtcgttt tacgatttgg
     1561 aaagctgcgc ttaaagttat aagaccaact ttgttatttg gttatggaga gaccgatttc
     1621 tatgggaata gcgatgcaaa atttttgaaa aaagtagatc ttgatactac agatgtgaat
     1681 gaactaaaaa gagcacatgg aaacatgcat aatggatttc ttcaagtact tgtatcaagt
     1741 ggtataattg cttttatatg tatctatgct ttctatgtgt taaatatctt tagtttactc
     1801 aaaacatttt ttagaagtaa agtgaattat gcgtttcttg gtattgtttt aatttttata
     1861 ctatctattt ttatagatga aatggtggag gctcatgtac ttttcaataa gcgcgatgtc
     1921 atttcaattg ttttctggta ttatttggga agcatttctt acttgtattt aaaaaaggag
     1981 aattaatatg aagacagctt tcattgcttg gactccttta catttgatta atatattaaa
     2041 tgtttatcaa aatttttacc gagaatcaaa aggagattta tttatttatt ctgaatttga
     2101 aaacgctaga agatatagtg agcgtataaa agaattaggg ctatttaatg atgttgtttt
     2161 tgtgaatcac cggaaaatcg gttcttcctt taaacgtaaa attaacttgt taactaacca
     2221 taatcaattt ttctcggatg atttatccgt ttatgatgaa atttttatac agggggaaaa
     2281 ttatttcaca aaaatacttt tctccaatgt ttacaaaaaa aatccagatg taaaactaaa
     2341 ttatatagaa gatggtttgg gtacttattt gaagaagagt atattttcaa caagtaaaaa
     2401 cgttcaaaga gtttttaaaa tattaaataa aaatagcatc ttcaaaaaaa aaattgacga
     2461 ttatt
//
LOCUS       DAZBTF010000165         2395 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000165 DAZBTF010000000
VERSION     DAZBTF010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.173"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            316..570
                     /locus_tag="ACHGNS_002957"
     CDS             316..570
                     /locus_tag="ACHGNS_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFN6938811.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="ACHGNS_002958"
     CDS             571..888
                     /locus_tag="ACHGNS_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFN6938812.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1447..2382
                     /locus_tag="ACHGNS_002959"
     CDS             1447..2382
                     /locus_tag="ACHGNS_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938813.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          861 a          339 c          453 g          742 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 actactttca aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa
     1561 ggatacaagt ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt
     1621 ctttctgaga gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt
     1681 gatatatata cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc
     1741 agtgaatatc tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta
     1801 ctaccatatt tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca
     1861 ggtaatcaag gaaatatgaa tattacaagt aacaaaacgt cacgtgtgct attggcgttg
     1921 atgggttacg gaattcttta tagagatgac caaaatggcc agattctaat aaatcgggct
     1981 gggcgttcat tattaatgaa atatagaaaa tttataccgg aagattttca aaacttacag
     2041 ttagaaaaca tgcgtttaga agatttattt agattagcag aagatgataa gtatagattt
     2101 gaagttttga aaattcaaaa gaatgaacta ttgaataagt tatatcctga gaatgcgata
     2161 aaagcacatt ttggtgatgc taatcatatg tatagttggt tcattcctaa aagatttaga
     2221 gggaaaatca atgctggaga tcaagtgaaa gtactcaatc gaaatagtac agatgtcgta
     2281 atgataacag atatctatgt agaagatgaa gatataatca aatatatgaa gcctgtgata
     2341 aaacttgtta ataaaaataa aggaacccgt ggtgctagtt aattttttcc tagag
//
LOCUS       DAZBTF010000166         2390 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000166 DAZBTF010000000
VERSION     DAZBTF010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.174"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            197..2059
                     /locus_tag="ACHGNS_002960"
     CDS             197..2059
                     /locus_tag="ACHGNS_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFN6938814.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2059..>2390
                     /locus_tag="ACHGNS_002961"
     CDS             2059..>2390
                     /locus_tag="ACHGNS_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFN6938815.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          860 a          353 c          465 g          712 t
ORIGIN      
        1 aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt ttaacagtac
       61 atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct ataagttgca
      121 cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta tattagcaat
      181 gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt tgatttactt
      241 ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa atgttagtgt
      301 tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc ggaagcaagt
      361 cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg atcgaatcaa
      421 attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc aaagtctccg
      481 aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg gaatgaaaat
      541 ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg tgctggaaaa
      601 taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt tttggatcat
      661 aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag agttcacgga
      721 ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg aatactttga
      781 aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa tcattcgaat
      841 gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat tactaaatat
      901 tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga ttggtcgtta
      961 tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg tcatgcagca
     1021 ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata tctatagtga
     1081 tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg tgatttataa
     1141 agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga cgaatctgta
     1201 tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat ataatccttt
     1261 taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc aagcagttcg
     1321 acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat atattgcctt
     1381 acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag ccgtctacat
     1441 ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg aagaattaaa
     1501 taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga ttgctaccaa
     1561 agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc ctttcatcaa
     1621 agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa taaaaaaaat
     1681 acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa cgtccatcaa
     1741 taaagatact ttagaagact ttagcaagga attgattttg aaagcttata cgatttatga
     1801 acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag cttttttgct
     1861 tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg cagaaggtcg
     1921 tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg aagcagtttt
     1981 tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct catatataaa
     2041 agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat cgaggaaagt
     2101 caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt agtagaacat
     2161 gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa tgagatgctg
     2221 aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt caaagaagta
     2281 tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa tttaaccgat
     2341 gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DAZBTF010000167         2118 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000167 DAZBTF010000000
VERSION     DAZBTF010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.175"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(81..1007)
                     /gene="cysK"
                     /locus_tag="ACHGNS_002962"
     CDS             complement(81..1007)
                     /gene="cysK"
                     /locus_tag="ACHGNS_002962"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFN6938816.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(1147..2034)
                     /locus_tag="ACHGNS_002963"
     CDS             complement(1147..2034)
                     /locus_tag="ACHGNS_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFN6938817.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          584 a          474 c          356 g          704 t
ORIGIN      
        1 ttctcatcat aaatttttgt gtgttctttt catctttaaa agttttttac ataaacctat
       61 ctggagaaaa accaagtgcc ttatatctct tggtaaaggg ctgtagataa ataacgttca
      121 ccattgtcgg gaaccacagc taataccttc ttgccttttc ctaattcttt tgcgactttt
      181 agcgcggcag caattgcagc gccagatgag atgcctacta aaatcccttc ttttcgccca
      241 acttctctag ctgtttccat cgcttcgtct ccactaatag aaaggacttt atcatacaca
      301 gaagtatcca gtgttttagg aacaaaacct gtaccgattc cttggatttt gtgaggtccc
      361 gcttctttcc cttctaaaat agcagattct gctggttcta ccccgattaa ttcgattttc
      421 ggatacacac gttttaattc tcggccagcg ccagtgatcg ttcctcctgt accgatacct
      481 gcaacaaacg cgtccaagcc atttactcca aatgcttgat gtatctcagg accagtcgtc
      541 ttctcatgca caagaggatt tgcttcattt tcaaattgta atggtaaaaa gtagccgttt
      601 tcttttgcca aacgttctgc ttctctgatt gatccagaaa taccatccgc accaggagtc
      661 aaaatcaact ctgcgccgta ccctttcatc aataaacgtc tttcgatact catcgtctca
      721 ggcatcacga tgattacttt atatcctttt gcaactccta ccatcgacaa tccgattcct
      781 gtatttccag aagtaggttc aatgatagta tcacctggtt tcagtagacc atcgtgttct
      841 gctttttcta tcatacttaa agcaatacga tcttttacac ttcctcctgg attaaaaaac
      901 tcaagcttta caaaaacatc tgctgaatct tctggtacga ttttatttaa tttgacgatt
      961 ggcgttttgc caatcaattc ggtgactgag ttatagatag ctgtcatctt caattcctcc
     1021 ttgtctttgc tcttcttact tatcataata caatctgttc atttatggcg agtgattcgt
     1081 ctttgactag caaaaaaaag acaaagatat accatatatg gtaatttctt tgccttttta
     1141 tctaaatcaa acgtttagtt cttgaccaac taataaagaa taactggaca tagacatgcc
     1201 atttttcgcc attagatcat caacagacat gccaaatttt tgtgcaatat cccatagcgt
     1261 atcacctgac acgactgtat atgtctgacc attatttgta tttacatctg ttgttgaaga
     1321 tgatgacgtt gaagtcgtcg tagtagtcgt tgttgttgta cttgtggttg ttgtagccga
     1381 acctgttgat ggcgtatcaa aacgggttaa attataagta ttaatcaatg agttcaattt
     1441 ggccccataa tttggatcgg tagcataacg acctgtcaag tacgctgttg catcataaaa
     1501 acttgttgta ttgcttttcc acacgccagc ataatgataa tcgcccgtgg aaaaactggt
     1561 tgaacgaaga acattcgcat ggtcttggaa ggattcccaa taagaagaat attttcggaa
     1621 tggctcattc atcgttaccc attgtccgtt tacatattct ttggttgcca tataaacgac
     1681 ctgtccgtta tagcttcctt tcacaccgaa tagattataa tttggcgcac tagctaatcc
     1741 ggaagtgcca tatgcacttt ccaacaacgc ttgagcgatc atgactgaag catacaagtc
     1801 attacttgca gcaacttctg acgcagacca gccgatttga tcgataaacg cttgaggatc
     1861 agcgactgtt tgagatcctt gttcatctgc atgaacatgt tgcggtaccg ctgtaaccat
     1921 catcggccca actaaaagac tcgttccaat taaaggagca atcgcttttt tattgatcac
     1981 catttttctt ttaggtctat ggtgccgggc actgcgggtc ttcagctctt gcattcaaat
     2041 tcctcctcaa aataaaaaaa cttaataaaa agttctcttc ttaaaacaaa cttaattaat
     2101 taaattatac ttttaatt
//
LOCUS       DAZBTF010000168         2059 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000168 DAZBTF010000000
VERSION     DAZBTF010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.176"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..426)
                     /locus_tag="ACHGNS_002964"
     CDS             complement(<1..426)
                     /locus_tag="ACHGNS_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFN6938818.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
     gene            complement(535..1566)
                     /locus_tag="ACHGNS_002965"
     CDS             complement(535..1566)
                     /locus_tag="ACHGNS_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN6938819.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          636 a          331 c          356 g          736 t
ORIGIN      
        1 aggaatctgt tttgattttg ttccatttga aatttctctc actcgctttt ttgtctcatc
       61 atcaaataaa atgaaagggt cttctaaacg atcgataaat acttccattg ttgttgttgg
      121 tagatgataa gaagtcatga caggcactgc accaaggtaa gacacagaaa cagctagaag
      181 gtatgtgtca aaagcggaac tcttataaat aataactttg tctccggatt tcactccaag
      241 atgagccaat tgataagccc gtttcaacac actttcgcag cattttttgt aagttgtttc
      301 aagtcctaat tctgggaaag ctgaaagtgg ctcatcaaag ataatcgggg tctcggaaaa
      361 gttagctgca gcttcttgaa aattcgtaaa taagttcaaa ggggaatacg ttatggtctc
      421 catcattcac actccattat cttttgttaa gtaatataca aattcaagat aatcgtaaca
      481 taatccttta tataataaca tagtttattg tgaaaaaaag atcatacatt tgttttatat
      541 ggaagaaacc cgaacgaatg ccgctaattc gtgataagaa aaatcaacca tcttttccca
      601 gatatcttga aaagaatggg atgagaggta tcgattacca cataaataat ttaccgcgtc
      661 agcatcatct aaatatagtt ctgcataata acgatatgtt tttctgcata gttctggcga
      721 tacgttttta tatactgtaa ttttgctcaa aatttggcga atagtccgag gagaaatttg
      781 ctgatcatgt gaggaacgaa aaacaaaaga attttcgtcc gtattttgtg ctgttaactt
      841 aaaatagttc tccagaaatg gcatcgcttc ttttggtatt ggtactttat tcagtctgtt
      901 ttcttttcta ttcagattaa gaatctgctt ggtcaaagat acatctttac gtttaagttc
      961 tactacttca tggatcttta atccgcaata cagaagaatg gcagtaattg caaggttcct
     1021 cggtctattc ttttcttcaa agctagcttc tctgcttgtt tgaaagcctt caatcgggat
     1081 aaacaaatgc tcaaaatctt tgaaaagttc tgcatcgata tatcgcggag ctgctttggt
     1141 tgtttccggt aaacttgtac ccaaaagaga aatctcttct tcaatcagtc ctttttgata
     1201 caaaaatcgt aaaaaatgat ttaatgaata ttgcttcctt gaaatcccgt aatagctatt
     1261 ttcaaacaat tgctccgttt cttggttcgc tgtagtttgg aacgaacgag tatgtcgata
     1321 gattttagct aagaaattct gataatcttg tttttctaaa tggataatca gctgatcatc
     1381 cacttcttca gaaaatactt ttctcaaata atcagtaaac agatgaaaat cttttgcgta
     1441 ttcatagcga ctgcctaatg aaagggaaga acgattttct tctaaaaact gttgaaagac
     1501 ggaagggagc aagtgtgtac gttgctttaa cttttcttgc agttgttttc tagttttgct
     1561 gtccattaca aattccctcc aatagtgttc attttctata ttttaccgca tttcatcaaa
     1621 tgaagataga cagtttgtct tgatcttagg aagcaaattt ttaactaagt actagctaaa
     1681 agatatgaag taagaagata catttttagt tattttatta gtgcagtcat tcttttgatc
     1741 ttttaattga tttatcctct ttttagctat gtggttactt catgagcaat gaaatggatt
     1801 tggttcgttc taatggcagg aggctctttc ttttatttcc gaaaaattag aaaacatttt
     1861 ttcagaatcg attggctctc catttattca ataatggttt gtctaacgat attgttggga
     1921 taattcttaa agaagtatta gaaatagttg gtacttcatt actttacatg aagatttatt
     1981 ctatggtagg agcaattata ttgagaatta atcctttttt atataagttg tttgcttatt
     2041 gatggattgg ataaattaa
//
LOCUS       DAZBTF010000169         1908 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000169 DAZBTF010000000
VERSION     DAZBTF010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.177"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..1085
                     /locus_tag="ACHGNS_002966"
     CDS             3..1085
                     /locus_tag="ACHGNS_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015076493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN6938820.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1303..>1908)
                     /locus_tag="ACHGNS_002967"
     CDS             complement(1303..>1908)
                     /locus_tag="ACHGNS_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFN6938821.1"
                     /translation="VEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVRSEELL
                     ALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLKTWKKT
                     QQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFLIEHNK
                     NIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
BASE COUNT          656 a          343 c          330 g          579 t
ORIGIN      
        1 atatcaatga tgatggctca agaaaaaaag tggagaaagt tggcggagac acccgaccag
       61 aggccgaagc tgctttacga aaagttttat cagatattga cgaaacagga caatactttt
      121 taggtacgga tactcgagta aaacaatacc ttgatttttg gatggaggaa tacgttaaac
      181 taaatctaaa atacaatacc tatgaaaact accgatttac catcaaaaat catataaacg
      241 gttatttagg aaagaaaaaa cttacggatc tctcccctgc tcttttacaa aatttcatca
      301 atgctgaatt taaaaagggt tactcgaaga aaacaatgac tattactcac tctgtcctta
      361 agaatgcgct gaatatggcg gtttatcctt ggggattaat caagcaaaat cctatgctgt
      421 atgtaaaaat accaaaatat gaagcacgac caacgactaa aaaagatcta aaaatcattt
      481 ctcttgagga ctttgatcat atgctagaaa ttactcctga aggacatcct ttctatattc
      541 ctttgaatat tggattttat acgggaatgc gcgttggcga agtttgtggt ctgacgtggg
      601 atgatgtcga tttttcaaat ggaacaatta ctgtagagaa acaaatggta aagaatgacg
      661 gcgcatgggt atatggtacg ccaaagacaa gcagttccaa tcgaacgatt tttattggac
      721 aaaccttgct agcaattctg aaaaaacata agaaacaaca attagaaaat cgaatgaagt
      781 atggaaagct ctacattgat tcaaatgcag tatgtacgaa ggaagacggt gagctagtta
      841 cgccaagtgt agtgaaatgg aatactagga ggatatcgaa tgcactctcc ctctctttta
      901 acttccattc tctcagacat actcatgcta cacttcttct cgaaaatggc gcaaaaatga
      961 aagaaatctc tgaacgattg ggccacagca gaatttcaat tacgatggat acttactcgc
     1021 atgtaacaga taagatgaga aatgaaacgg tcgatatcat ggaaaatctt agaaagaatt
     1081 cttgattttt gccaccgaaa aaaaccaccg gtggcaaacc ggtggcaaat ccatgtaaac
     1141 atggtttatt ttttacactg ttttcaaaca aacccttgct atttcttcgg ttcgtcctcg
     1201 tccattttcg ataccgacag ctagcttata acccaaggta tatcaacata aaaaaataaa
     1261 aataggctat gacagcagcg tgacagcaaa aaagatggaa agttaaatat caaacctcgt
     1321 aatttcattt gcgagtgctt gatttgtttc tggatataag tgtccatatt tgtccaaagt
     1381 agttttaata gaggcatgac ctagacgctt agatattgca tagatatttt tattatgttc
     1441 aattaaaaag gcaacatgcg agtgtcttaa tccatgcata tgaattggtt ttactcctgc
     1501 aatttcagct agttttttta ttctgcttaa aatagttctt ggagatggcg gtaatccatc
     1561 aaaagaaaaa accaattcta tatttttccc aattgattgt tgagtttttt tccatgtttt
     1621 taaatcttca acggtttgtc tgtctaacga aattatccga atacttgatg tattttttgt
     1681 ttcagcaaat tcataatctt gtcttgtacg gatgtaaatt gacttattta cgctacatct
     1741 gccatttatt aagtcaaagt ctttccattg taacgctaag agctcctcac tccttacacc
     1801 tgtaacaaaa ataaaacgca tcaaacgttt ataaaattct tcataaaaat ccccgtgata
     1861 aacactatta tatactttct taaattcatc aactgtccaa aattctac
//
LOCUS       DAZBTF010000170         1890 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000170 DAZBTF010000000
VERSION     DAZBTF010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.178"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..242
                     /locus_tag="ACHGNS_002968"
     CDS             <1..242
                     /locus_tag="ACHGNS_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN6938822.1"
                     /translation="TLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNYY
                     VFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            334..1839
                     /locus_tag="ACHGNS_002969"
     CDS             334..1839
                     /locus_tag="ACHGNS_002969"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFN6938823.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLEFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
BASE COUNT          622 a          351 c          370 g          547 t
ORIGIN      
        1 acacattgta cacagtcggg gcctcaaatg gcggtataac gataccgccg attattttcc
       61 acaaattttt tcggcttcca atgccgcaag gacttttact taccaatagt cttatcgtct
      121 ttttgaatta ttatgttttt ggattgttgg agacactatt cgttctccta tctattacag
      181 tcagttcatt gtttatgaaa atactcatcc gcttgtcccc tgtgtcaaga aaatttgaat
      241 aaagctggat tttgtcatag cggaaacaaa cgatatatta aaaagagtgt gctataataa
      301 accaagaaat tgaataaagc gagggatcaa tcaatgagct tctctttaga agacatccgt
      361 catttactat taaaagaaaa tctattaaaa gaatttgttt caacaagagg ctggcatctt
      421 gatgtaccag aaaaagctga atttcttcag ttaagttatg attcaagaaa agctgatgct
      481 tctactttgt ttttctgtaa aggaatgaat ttcaaagaag aatatttgga ttcagcaatc
      541 gaacagggta ttcaatatta tgtatcagaa caaccatacg aaaattcagc agtaggaatc
      601 atcgtaacgg atatccgtaa agcaatggcg cttcttgcta tggcttttta cgactatccg
      661 caaaataaat tgaaagtgat cggattcact ggaacaaaag gtaaaacaac tgctgcgtac
      721 tttacaaaag caatcttgga tcacacaaca aataaaaaaa cagcattgct ttctactatg
      781 aatacaacgt tagatggcaa aacatatttc aaatcccatt tgacaacgcc tgagtcatta
      841 gatttatatc gtatgatgtc agaagcagtt gaaaatggta tgactcatct agtgatggaa
      901 gtttcttcac aagcttacaa aacacaacgc gtatacggct tgactttaga tgtcggaatc
      961 ttcttgaata tctcgcctga ccatatcagt ccgatcgaac atccgacatt tgatgactat
     1021 ttctattgca agcgacaatt gatcttgaat tcaaaaacag ttgttttaaa tcatgaaagt
     1081 gactattttg atttacttag ggaaacagcc gatctattcc agattccaac gatcacttat
     1141 ggtcgttcgg aagaaagcaa ttatcaagtg atccgttcag ataaaggtac tcacggtttt
     1201 actttatctt ctcatgaaga tcgcttagct atctgcgata ctgcttatga tattttgctg
     1261 gctggaggat tcaaccaaga aaatgcagcc agtgcgatca ttgccgcagc actaacaggt
     1321 gcctccagtc aagatgcaca agacggatta aaagaagctc gtgtaccagg ccgaatggat
     1381 caattgatcc aatccaatgg agcgcatgtc tatgtggact atgcacataa ttatctaagc
     1441 ttgaaaacat tgctggaatt tgccaaaaat gaacatccag atggccgtgt aattgttgta
     1501 ttaggcagcc caggaaacaa agcaatctcc cgtcgacatg attttggaaa agtgctatca
     1561 gaaacagctg acatcgcctt tttgacggca gatgacccag catttgaaga tccgaaaaaa
     1621 atcgcggaag aaatcaatga agcaattact aatccagatt tgatcgttca ttatgaaatg
     1681 gatcgtcctg aagcgattcg acgcgcttta gcagaaagcg ctccgcaaga ttcagtcgtt
     1741 attgcgggaa aaggcgtgga tccctatcaa aaaatcaatg gtgtagatga accttatgaa
     1801 ggggattatg caatcgtgaa gcggttgatt gaagaataat ggtatttata tactagtggt
     1861 ttcccatttc taagaaaaaa agggactgtg
//
LOCUS       DAZBTF010000171         1839 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000171 DAZBTF010000000
VERSION     DAZBTF010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.179"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..537
                     /locus_tag="ACHGNS_002970"
     CDS             <1..537
                     /locus_tag="ACHGNS_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938824.1"
                     /translation="KIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYGIDET
                     DIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLISP
                     ISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKI
                     LLPENWAELRKLVEYGII"
     gene            524..>1839
                     /locus_tag="ACHGNS_002971"
     CDS             524..>1839
                     /locus_tag="ACHGNS_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFN6938825.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHIL"
BASE COUNT          610 a          235 c          283 g          711 t
ORIGIN      
        1 aaaatagatg aaaacagtaa attgatgaaa aacatacaat caatctttgg gatagaagaa
       61 gtttttgtcg gagataaatg tatctttttt gatcaacctt tattatctga tggttatggc
      121 atagatgaaa cagatatttt cgctaaagta aatagtatat tttctggaaa aaaagaaact
      181 ttaattaagc ttcatcctag agcaaggaca aatcgttatg gtgaaaaaaa catattgaat
      241 tccagtttac cgtttgaagt cttgctactt gaaaatgact tctcttcaac acttttgatt
      301 agtcctatat ctacaatttc tttttcacca tctatgatgt ttgaaaagac gatcaactct
      361 attatgttgg ctaaattaat tttaaatgaa tatacagcat tatctgaaga taaaaaagaa
      421 acattgacaa atattgttct tttctgtgaa cattataacc gtctatcaga agaaaaaatt
      481 ttactcccag aaaactgggc agaattaaga aagttggtag agtatgggat tatttaacac
      541 actttccaag tcaaaattag ttaagtccag tttctggtat actataggga atttttttat
      601 taagggaatt tcattcataa ctattcctat ttttgtaaat attactactc aagcagaata
      661 tggtttgatt aataatttca cttcatttgt atcaattttt tcgattcttg ttgggctaag
      721 cctaaattcg gcaattaata atgcaagttt tgactataaa gatgacataa atgaatttat
      781 gtcatcggta ctttctctag ctacactttc gttaattgtg ttcttattaa tagggaacat
      841 atattttttg ttttcaaatg ctttttttga tatgaaccaa cttgtattta atttgatgtt
      901 attacaaagt tttgctagtt ttgtaattag ttatatccag tcttatttta cgattaactt
      961 taaccatttt aaatatttga ttttatcttt tatatctact tttttgaata ttgcaatctc
     1021 tgtagctttg atgcagacat tatattttga tgataaatat gttggcagag tagtcggcgg
     1081 aacactgtca tttgtgttga taggtttagt aatttattta agaataatga taaagggtgc
     1141 tcattttttt gataaaaaac agtggagata cgctttaaaa atctctttgc cgctaatccc
     1201 acattcctta gcaaatatag ttttagcaca attcgataga attatgatta attcttattt
     1261 aggtcctgct gcggggggaa tatattccta cattagtaac ataggtatta ttctaagcgt
     1321 aatatggatg tctactaata acgcctgggt tccatggttc tatggtgaga tgagtaaaaa
     1381 taatgaaaaa aaagtgaaga aagttagtaa ctattattta gtagtcttta ctttgattac
     1441 gatgattgta atggtagttt cgattgattt agcaaaattt atggctccga aagactatta
     1501 ttctgggtta tctttagtgg ttccgataac cttaggctat ttctttcagt ttttatatag
     1561 tttaccagtt aatgctgaat tttatgagaa aaaaacatcg tttattgcga ttggtacagt
     1621 agcaagtgct atagttaata tactattaaa catgattttc atacctaggt taggaataat
     1681 tgctgctgga tacacaaccg tatttacgta ttttttactt tttatttttc attataatat
     1741 cgctaaaaaa attacgaata aacaattatt tgacacaaaa aaaatatgta tcataataat
     1801 ttttgtatca attttttctt tgatacttca tattttaat
//
LOCUS       DAZBTF010000172         1725 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000172 DAZBTF010000000
VERSION     DAZBTF010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.180"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(60..284)
                     /locus_tag="ACHGNS_002972"
                     /pseudo
     CDS             complement(60..284)
                     /locus_tag="ACHGNS_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(838..924)
                     /locus_tag="ACHGNS_002973"
                     /pseudo
     CDS             complement(838..924)
                     /locus_tag="ACHGNS_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          606 a          253 c          229 g          637 t
ORIGIN      
        1 ggtattttta ttatatatag atttaacata tgtcggtcgg ttgattgata tagctaaagt
       61 tataactcaa ccgaagccga ttcatctaca cgaataaagt tgcgagtgtt ctcggctaat
      121 tcataaaaat tgttatgtgt tccatatagc aatactggaa taactttatt ttcttgaatc
      181 tcataagcaa ctctatacac ggtgaccgca tacttaaatc cattagacca attacctgat
      241 aaacctccag ttttttgctc ttacttaaat ggatcaatgg caatttcatc acaaacctat
      301 agaggatgag tgacttctgc ctaattacgt taaaaaatat tgaaagtaac tctatgtagc
      361 aaaaaatatc tagaatttca tttcctcaaa actgatcgat attgtatcta aaaatatata
      421 ctacctaaaa taggtagtat atattaaaat atgtgctttt gttagaaaaa tcaatacata
      481 aattatcata tctgtatatg tcatttaggt atttcagtta tgttataata gttttgctgt
      541 ctgctacccc ggcctaatct ggcaagggga ggaggagata taatgaaata cctttttgat
      601 tttgtttttg caatcaagcc acttgcagat gtagtgtgca gttacctctg caaatggctt
      661 gattgcaaaa ttgccaaaaa agacagtaag cctacccgtt aattctataa tatagaaacg
      721 taaaaaaaac cccatcagcc tgcacgctga tggggtttgg tgatataatg aaatacctct
      781 ttgttactca taattatacc ataattagtc acgaatgcaa ccaatagaaa aaactactta
      841 gaacggtaga tcatcgctaa taacaatgca ttgtccacca aatggatttt gtgaagcatt
      901 aaatacattg ttattaattg tcgctgcttt taactaggcc tctccccttc tattataaaa
      961 gaagatatat atcatttctt aattacctac taccttaaac caaaatttaa gtactaccta
     1021 atttaagtag tacttaaaca atcaaaaaaa tagttatccc aattgttatg cttttataat
     1081 acgattattt gtaacaaaat gaatattata taattaaaca ctaccatatt taggtagtgt
     1141 ttaattatta gcaaaacaaa aaatatttat tctattctct taacttcaac taagttatgc
     1201 tgcacacctt tatataaatc atatgcaact cattagctag acaaactaca agcatgtagt
     1261 tttatctgtt aattatgtac tacctaaata aagtagttta taattagcaa gtatatatat
     1321 ttaaaaattt aagattaatt tttttatgac ttgtacaaat ttatctgata ttctcttatt
     1381 aagaactacc taatttgggt agttcttatt ttttacattt taagtagtat ttaattgcaa
     1441 tttggtagta tttttttatt atcttattct tagaacggta gtacttaata taaatatagg
     1501 tagtatctaa tttaagtatt aatggtagta tctaaattaa caggtagtat gtaactactt
     1561 taaagtaatt tttttcagat taacacagta gtatcttatt aaataagtta attttaatgg
     1621 tagtacataa ttattatata tatctgacat tggtagtact tattgtattt tttagtagta
     1681 tataatctat tgttttctag cctaaagcct tgtcttataa gaata
//
LOCUS       DAZBTF010000173         1683 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000173 DAZBTF010000000
VERSION     DAZBTF010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.181"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            216..1154
                     /locus_tag="ACHGNS_002974"
     CDS             216..1154
                     /locus_tag="ACHGNS_002974"
                     /inference="COORDINATES: protein motif:HMM:NF016640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase domain-containing protein"
                     /protein_id="HFN6938826.1"
                     /translation="MGRQELYSLAINELEKRNLEIHELNQKNNSILGMKIRNKINQLN
                     QMKKRDNLLPAKYTLKSMTGKGPIKDYSNRENRSDELRKKELTNQGTPKRIAVYSCIT
                     GNYDSIKEPDFSHPLVDYYLYTDNVITSEHWNIREIPKYISELKNNILINRYIKFHPN
                     ELFTDYDFTLYVDGNVTITSDITGLLNSSSKLGLSMHKHSERKSLYSEADALIAVGKG
                     NKESIIEQISKYREEGFPDNFGLAEATVILVDLNNSIAENLLDLWWREYLNSESGRDQ
                     LAFPYVLWKSGHAMKEVTTLGNNVYKSAKLRIISHY"
     gene            1406..>1683
                     /locus_tag="ACHGNS_002975"
     CDS             1406..>1683
                     /locus_tag="ACHGNS_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFN6938827.1"
                     /translation="MKILITGGAGFIGFHLSRKLLKETENQIVAIDNLNDYYDVQLKE
                     DRLTILYDYNNFKFIRMDLLEKEKLDSLFESEGFDIVIHLAAQAGVRYS"
BASE COUNT          639 a          192 c          302 g          550 t
ORIGIN      
        1 taatgagtaa ttataagaga acaaaagtaa agaattatat ctacttgctt gcgtatttct
       61 tttttatcat tgtgtgtttg atattcatca atataagagg aactgctttt gtaaatggaa
      121 tcctaccatt atttagagaa aatattattt ttaaggctgt ttactatttt tttgagtaag
      181 aataaatagc acttaaaaat agagatgagg gatttatggg aagacaagaa ttatatagtt
      241 tagctatcaa tgaattggaa aaaagaaatc ttgaaataca tgaattaaat caaaaaaata
      301 attctatttt aggcatgaaa atacgaaata aaattaatca acttaaccaa atgaaaaaac
      361 gagataatct gttgccagca aaatataccc ttaaatcgat gaccggtaag gggcctatta
      421 aagactattc aaacagagag aatcgcagtg atgaactaag aaaaaaagag ttaactaatc
      481 aaggaacacc taaaaggatt gctgtgtata gttgtatcac tggtaattat gattctatta
      541 aagaaccgga tttttctcat ccattggtag attactattt gtatacagat aatgtgatta
      601 caagtgagca ttggaatatt agagagatac ctaaatatat atcggaatta aaaaataata
      661 ttttgatcaa tagatatatt aaatttcatc ctaatgagct atttactgac tacgatttta
      721 ctttgtatgt tgatggtaat gtaacaataa cttctgatat taccggatta ctgaacagtt
      781 cttccaaact tgggttaagt atgcataaac atagtgaaag aaaatcttta tattctgagg
      841 cagatgcatt aattgcagtt ggcaaaggta ataaggaaag tattatagag caaattagta
      901 agtacagaga agaaggattt cctgataatt ttggattggc tgaagccacg gttattttag
      961 ttgatttaaa taattcaatc gctgagaatc ttctagattt gtggtggaga gagtatttga
     1021 actcagaaag tgggcgagat cagctcgcat ttccttacgt gttgtggaag tccggacatg
     1081 caatgaagga agtgactacc ttaggcaata atgtatataa aagcgctaaa ttaagaatca
     1141 taagtcatta ttagccctaa atgatttcta aactattctt tcaaatccta gtaaatattt
     1201 ggagatagaa gatttccttt ttaaagtcat aaaaaattaa gttatatttt ttgagtgaat
     1261 ggagaagacg catagtatca taaacttcgt tatatcttaa attatggggt atgcctaaat
     1321 aataaatgta atacgagtat aaagaagaaa gcaattttgg gtttaaccct attggttcct
     1381 ttcagaaaaa ggtggatgaa taaaaatgaa aatattaata acaggtggag caggatttat
     1441 tggttttcat ttgtctagaa aattactcaa ggaaactgaa aatcaaattg tagcgattga
     1501 taatctcaat gattattatg atgtccaatt aaaagaagat cgattaacga tactgtacga
     1561 ttataataat tttaaattta tcagaatgga tctattagag aaagaaaaat tagattctct
     1621 atttgaaagt gaaggttttg acatagtaat tcatttagct gctcaagcag gtgttagata
     1681 ttc
//
LOCUS       DAZBTF010000174         1664 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000174 DAZBTF010000000
VERSION     DAZBTF010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.182"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACHGNS_002976"
     CDS             131..505
                     /locus_tag="ACHGNS_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFN6938828.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACHGNS_002977"
     CDS             complement(619..1248)
                     /locus_tag="ACHGNS_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFN6938829.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACHGNS_002978"
     tRNA            complement(1382..1454)
                     /locus_tag="ACHGNS_002978"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          284 g          556 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtcgt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DAZBTF010000175         1649 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000175 DAZBTF010000000
VERSION     DAZBTF010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.183"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            138..1604
                     /locus_tag="ACHGNS_002979"
     CDS             138..1604
                     /locus_tag="ACHGNS_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFN6938830.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          462 a          296 c          338 g          553 t
ORIGIN      
        1 tttttatgat attgccatta aaaattactt gcttttatag aaaattagtt tatatttctt
       61 aaataagttt tttagttaaa tttttcggta aagatgtata atattttctg aaaatttaaa
      121 gaattggggt atagattatg gaaaaacagc aagacaaggg cttcttcggg cagccgaagg
      181 gattgtcaac tttgttcttc actgagatgt gggaacgatt tagttattac ggtatgcgtg
      241 cattacttat ctattatatg tacgatacag ttgccaacgg gggacttgga ttaccacgag
      301 caacagcatt agctatcatg tcgatctacg gatctttgat ctacatgtct agtatcgtcg
      361 gcggatgggt atctgaccga gtgttaggag caaaacgcac tgtattcttc ggtggagttt
      421 tcattatgct tggccacatc gtcttggctt taccatttgg tgtaagtaca ttgtttgttt
      481 cgatggcatt gattattatc ggaaccgcct ttttgaaacc aaatgtttct ggcatggtcg
      541 gacatcttta ttcaaagact gacttgcgaa gagatgcagg attttcgatt ttttacatgg
      601 gtgtcaactt aggggcatta ttagcaccac taatcgttgg tacaatcggt caagaatata
      661 actatcattt agggttctct ttagctgcta ttgggatgtt ctttggatta ttacaataca
      721 tcatccaagg acgcaagaca ttagacggag tgggtacaac accgccgaat cctttgacaa
      781 ctgaagaacg taaaggattc atccgtaact tgatcatcgc acttgttgtt atcgctgcag
      841 tattcggcgg ggcacaacta actggtcatc tgacaattga cttttttgtc aatacaatca
      901 gtgtattggg gattatctta ccgttgtatt actttatcaa aatgctgact tcaaaagatg
      961 taactgctga agaaaaacca agagtatggg cttatatccc attattcctt gcagcaatca
     1021 ttttctggtc attacaagaa caaggttcat ctatcttagc tttatttgct agtgaacgta
     1081 cacaatctac gctatttggt gtaacgatcc aagcaagctg gtatcaatca ttgaatccaa
     1141 ttttcatctt tattctgaca ccagtattcg taacgatgtg gacgaaactt ggtgaacgtc
     1201 aaccatctac agtagtgaaa ttctctcttg gacttttatt tgcaggatta tcgtacttgc
     1261 tattgatgtt gccgggtatg ctttatggta caagtggaaa agtaagtcca ttgtggttgt
     1321 tgggaagttt cttcatcgtg gttattgctg aattgtgtct ttcaccagtt ggtttgtctg
     1381 tgacaacaaa attagcacca cgagcatttg aatcacaaac aatcgctatt tggttcttag
     1441 ctgatgcttc ttcacaagca atcaatgcac aaattgcacg cttctacaca ccgggaacag
     1501 aatctgctta cttcggtatt atcggtgcag tagcaatcgt cggtggtatc ttgctattta
     1561 ctgtgaaaga accaatcaaa aaactaatgg gctctattca ttaatcaatg aacataaaaa
     1621 caggaattgc ttatttgcag cagttcctg
//
LOCUS       DAZBTF010000176         1627 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000176 DAZBTF010000000
VERSION     DAZBTF010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.184"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1604
                     /locus_tag="ACHGNS_002980"
     CDS             <1..1604
                     /locus_tag="ACHGNS_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFN6938831.1"
                     /translation="ETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHK
                     MFRPDNAYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPI
                     REGRTMEDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYN
                     GDINNRLARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFS
                     RTNRTYPEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQA
                     YGKLKEVSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQ
                     IQLLEEQIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTY
                     AANSSDAREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQAS
                     DNLIHAFSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFK
                     YPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          595 a          267 c          332 g          433 t
ORIGIN      
        1 tagaaacata tgcagagtat tcaccggaac agatcaatcg tatgatcgat caaatggagt
       61 ctgtcaaaaa agaaagttat ttggacccta ctgtttttga agcagaggaa catatccaaa
      121 aagtcattca taaaatgttc cgtccagaca atgcctatac aaaatttgat tttcgaaatg
      181 gccgtccaac gaagtctgcg atcttaacca cgagttccat tgatatggcc aaaaagtatt
      241 atcgggccat caaagagatg acgaaagaat ctgattggtt aataaaagag tttcccaatc
      301 agcctatccg tgaaggacga acgatggagg atcctgattt cccacgaatc gctattacct
      361 attcattaga agaaaatagc cgagattcaa gtgcccaaca agaagaaatg caaaaaatta
      421 ttgaggaata taatcagtat tatgatactg cctggtcact agcagacatt gagcgttata
      481 acggagacat caataatcgt ttggcacgaa aaagagcgga attcaaacaa tttgggaaac
      541 aaatcgattt agtaatcgta gtggatcgtc tattgacagg ttttgacgcc cctacgattc
      601 aaaccctatt tgtggatcgt aatctagagt atgcaggatt gatccaagcc ttttcacgta
      661 cgaaccgtac gtatcctgaa aaaacaaaag gactaattgt tactttccga aagccagcga
      721 caatggagaa gaatgtcgag gatgcgacaa aactgtattc agaagctaaa gaagaatcgg
      781 gtttgattta tccaagttac caagaatcca aaaaaagatt caagcaagca tatggtaagt
      841 taaaagaggt ttcatccatg gaatccattg atgaacatac gccacttgaa atccgtatcg
      901 agtacgtcaa agcatttcaa gaattgaatc gtgcgtatga atcgttagtg acttatgatg
      961 attacaatga tgacatggtg caatcttcaa ctttgaacaa tcaaattcaa cttttggaag
     1021 aacaaattgg cgtgtacgag accgtgaagg gttcattgat ggaggaagaa ccagaaaaag
     1081 agggagaaga tttttcaacg atcgaatttt atagtgaaaa taccactaag ttgtatgata
     1141 ttgattctac ttatattgat caattactag gaacttatgc tgcgaatagt tcagatgcac
     1201 gagaagaaat tgaaaaagct ttggctaaat tgaataaaac agaaggagtc aaagaagttt
     1261 accgacaaat tttaaatgca tttgatgtgg ggactttaga tagtaatgaa gatatctttg
     1321 tcattaaacg tcgctatttt acacaagcaa gtgataattt gattcatgcc ttttccaatg
     1381 agtggtttgt ttctgagaaa gaattacatg cttctaatct gcaatatatg ccaggtgaag
     1441 atccaattcc taatatgaaa gcaattatca acagtaagga ttacgaagga tataaagcca
     1501 aacatccaga agcgaaacca tttaaatacc cacaagaaat gaagcgagca tggcgtaaaa
     1561 tgttagatga tgagttgatt ccacttgaaa atgaattgag gtaaacgaga agagttaaga
     1621 aaattgt
//
LOCUS       DAZBTF010000177         1622 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000177 DAZBTF010000000
VERSION     DAZBTF010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.185"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            17..277
                     /locus_tag="ACHGNS_002981"
     CDS             17..277
                     /locus_tag="ACHGNS_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938832.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(639..878)
                     /locus_tag="ACHGNS_002982"
     CDS             complement(639..878)
                     /locus_tag="ACHGNS_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938833.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            1164..1478
                     /locus_tag="ACHGNS_002983"
     CDS             1164..1478
                     /locus_tag="ACHGNS_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938834.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          558 a          219 c          286 g          559 t
ORIGIN      
        1 taaggatgga tgaaatatga ataacgaaac tatcatttta ggaaaaaaat atatttgcca
       61 acctattggc ttgaaacaaa aagtgattgg agaagtaatc agtaaattag aaaattgtgt
      121 tgtgatctgc gtagacgaat atcatgacgt cgatcgcaat gaaatattag aaaaatgcgg
      181 aaaagtagtg gcaaaatata ctcacgttta tgagttggaa acaacagaat atctatataa
      241 ctatagcaac aaatatgaac atactttagt ttcataaaga tttaaataaa gtgccttcaa
      301 aagcgagaag accaagcttt tgaaggcact ttatttagtt ggcagtgatt gttcatgatt
      361 tttgatttat tcaaaaggtt cgcctagaga atctgtctct gtaacgaggc ctggtggaga
      421 aactcgataa aaacctatac tgccagaact gtcctatttt cggatagaca aactagtagt
      481 tttaaaaaga tcatccgatc caatcatttc aaaaaatgca aaactgatat caggatcatt
      541 tagctcaggc ttgttatcgt aaagcatttg taaaggttgc atttagttag gagtaaacgc
      601 gaaactatca tcattatttg ctgaagaaga tgaagaagct aattcagagt ttgtttgaaa
      661 taaaggagac gtgctgacag tttctttacc ctgttttttg cagaggtaga aacttttata
      721 tcggaagttt ctaccggaga atttttctca tttgtagaaa ttgttgaatc gatactattc
      781 gaaatttgcg tatctgaagg tatgttcttt gatgcagaag tcccacaagc agtaagtaac
      841 aataaatttg ataaactgat aaacagaatt cttttcatta tgtacacact ccttatagta
      901 tcgtttagca tacacctatg ctcagtatta ggcaaaatca atgcaaaact atcaagtctt
      961 ttatagtatc tattagtctt agtaatacat atatggagag ggtgatatcc cagttcaaac
     1021 cagatacatt ggttaccatc tgtattaacg attatgcctc taaacgcttt ttttatgaaa
     1081 aaatttataa gtgaaaacgt aggtatgata ttatctagag gtaatttttt tgggtattta
     1141 aatagttgct aggaggatag cttttggatt tttacagtaa ttttatctta ataatagcaa
     1201 tattattact tctaaatatt tggttttttg ataagagtag aaatgcaggg ataggtttta
     1261 gaaccaaacg aagtacgtct tctgaaaaaa agtgggtata ttcacagact attttttacg
     1321 gaggggttat ttcaatcagt ttactttctt caacacttta ttcttttaat gttatagacg
     1381 tttccatgtc aaattttata tctattattg gaatccttat ttctgcaatt attacacaac
     1441 tacttcttgt gtttgaagag aagtcaaaaa ataattaaag caattatgcc ttttagtaca
     1501 ggaattatta cttcttacaa atgtatagcg aatgtgaagc ctgctgtttt aattgttcta
     1561 gaaaaacttt tgagtgaaca attattttat gttaggatga attaatttct ctagagttta
     1621 ta
//
LOCUS       DAZBTF010000178         1512 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000178 DAZBTF010000000
VERSION     DAZBTF010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.186"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1077
                     /locus_tag="ACHGNS_002984"
     CDS             <1..1077
                     /locus_tag="ACHGNS_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HFN6938835.1"
                     /translation="INLLKKEKEDLNDEINQKYDETLKNVYSSLIVSNIEETSSSEIK
                     NKLSLLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGLLNKLTISNID
                     STREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITEEKEQQKAIKE
                     QMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKELYANKIQELEE
                     KLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEPMDRIKELSSA
                     SVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLDEIEKVVLENY
                     NGTVTFTKLAKAEQYRRSLELSKD"
     gene            1149..>1512
                     /locus_tag="ACHGNS_002985"
     CDS             1149..>1512
                     /locus_tag="ACHGNS_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFN6938836.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQDMLDYYY"
BASE COUNT          667 a          172 c          234 g          439 t
ORIGIN      
        1 attaatttat taaaaaaaga gaaagaagat ttaaatgatg aaataaatca aaagtatgat
       61 gaaacattaa aaaatgtcta ttcttcactt atcgtttcaa atatagaaga aacttcatct
      121 tctgaaataa aaaataagct ttctttgtta aaattaaaag aaaaggaata tattattgac
      181 gatgctgttt ctgtccctct tattttaaat aaacgagaga aaaataattt aaagaaaaaa
      241 atgctcattc catttgattc agaagtgaaa ggattactca ataaactaac tatttctaat
      301 atagactcga ctagggaaaa aatcataaaa atatttgata aaattaataa tttattcaag
      361 aatgacaatg ctgaattaag gaagggtctt ttagagttta aacttcaaga actagaactt
      421 cattactctt atttagtgaa aattaccgaa gaaaaagaac aacaaaaagc aataaaagaa
      481 cagatgatag aagaggaaaa agttcgtaga gaaattgatc gtgaaaagaa acgtattgat
      541 aaagaacaac gtcaattcaa ttctgaaatt tccaaattaa tcgcatatat gcaaaaagct
      601 aatgcagatg tggaaaaaga attatatgca aataaaattc aagaactgga agaaaaatta
      661 aaagaattag aagttatacg tgaaaatgta ttacaaagag aattaaatac tagagccggt
      721 tatgtgtatg taattagtaa tattgggtct tttggtgaag acatatataa aattggtatg
      781 actagaagat tagaacctat ggatagaata aaagaattga gtagtgcatc ggttccattt
      841 gaatttgacg ttcatgcgat gattttttca gaggacgctc cgtctttgga aactaaattg
      901 cataatcatt ttagaaaaca agaagttaat aaaattaatc aaagaaaaga atttttcaaa
      961 gtttcactag acgaaataga gaaagttgtt ttagaaaatt ataatggaac agtaacattt
     1021 acaaaattag ctaaagctga acaatataga cgatctttag aattgtcaaa agattaaata
     1081 tataaaaagc cttcgggctt tttcttttaa caattaatag gaacgtatat tcgaaaggaa
     1141 tagaaataat gtggttagaa aaaataaatg acaaaaaatt taagtacacc gaacgttata
     1201 cggatccctt aactgaaaaa aagagaaaag tgtccgttac tttatctagc aattcacgcc
     1261 aagcttggaa tcaagctaac ttacttttga atgaaaaaat cgcagaaaaa ataaaacgaa
     1321 atgaagaatt accactaact tttggagaat taaaaactaa atgggatgaa aaatacaagc
     1381 caactgtaaa agaaagttcc tatcgaacaa ctcaggttta tctatcatta atttcaaaat
     1441 atataaaaga tgatgtactt gtaaaaaacg ttaatagtaa tcttatacaa gatatgctag
     1501 attactacta tt
//
LOCUS       DAZBTF010000179         1480 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000179 DAZBTF010000000
VERSION     DAZBTF010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.187"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..972
                     /locus_tag="ACHGNS_002986"
     CDS             64..972
                     /locus_tag="ACHGNS_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFN6938837.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..>1480
                     /locus_tag="ACHGNS_002987"
     CDS             972..>1480
                     /locus_tag="ACHGNS_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938838.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGV"
BASE COUNT          529 a          197 c          316 g          438 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taaaaacact gaagttatgg aagctacaat tgtcgaaaat
      181 ggtcttccat atgatttatc agaatgtaag gtatattttg aatcagttgt tggcggaaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat caaatatatt
      301 tttgataaat attccatgca gtgtttgcat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcatcaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaattg aagatttaat tgctgatatg
      481 acagatttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgatc gctggagaga ggaacaggag aatagtttcc aagattggag agaaggacaa
      601 gaatcagatt acttgtcatg gtttgaatca atcaaggata ttttgaagtc tatcgatcca
      661 ggtggagtaa tgttagccga attaatggat gcacgtgtag atcttcaagg tgtacgccat
      721 gaatcgattt ctgaacgttt cctatccgat ctaaattatt tgtatcaaaa aatgaaagca
      781 gcacttttca cgattgaata cggtgaaatt gaagtgactg atattcttca agacgatctc
      841 ttttcagata atcacgaagt cgaaaaaatt gaaacagtag aatttccaat tgaagaagga
      901 gctttgatta ttgcaactgt tgatgatcca aaacagaatg ttttcactct tgagaaagtt
      961 ggggtgattt gatggttaaa gtaaagagaa tgatggaaac tgatgaatct ggtgttaaac
     1021 gtcaatttat gccaattaca catgtctctg cagttttagg cttagaaaaa atgatatcag
     1081 gacaatcaaa agttctttcg gttaatggta aatatggtgc agtcatttta acgaaagctg
     1141 atttaggatt agagaatgca attactgaac taccttatgc aagcgagaca agcgatggta
     1201 ttcttactgc tgaaatgttt caaaagatcg taaatggaga aggtggaaca tatattttgc
     1261 cgattgctac tcctgaacaa ttaggaggta taaaagttgg ggaactctta gaagttactg
     1321 aagaaggtgt tttatctgca actaagcaaa cagacttcaa tttctctgaa gaattaaaag
     1381 gaaaattaga atctctaaaa atattaaaag caggtgcaaa tatatcaatt gcagaagatg
     1441 gaacaattag ttctacaggt ggtagtggga ctggtggtgt
//
LOCUS       DAZBTF010000180         1459 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000180 DAZBTF010000000
VERSION     DAZBTF010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.188"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..613)
                     /locus_tag="ACHGNS_002988"
     CDS             complement(<1..613)
                     /locus_tag="ACHGNS_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFN6938839.1"
                     /translation="MDNHTRKLLVLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL"
     gene            901..>1459
                     /locus_tag="ACHGNS_002989"
     CDS             901..>1459
                     /locus_tag="ACHGNS_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN6938840.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDR"
BASE COUNT          505 a          213 c          279 g          462 t
ORIGIN      
        1 gcaggaagta ttgagttagt ttgtaaagtc tgcgatcttc aaggatcgta aatagctgat
       61 gggttgtccc atcaacacaa ataaaactca tggaaccaga aacagacttc attgatttga
      121 actcatccat acacaacacc gtcggaagga agttcatacg tggctggtaa tgcttcgttg
      181 cttcacgcaa gatccgtgtc accgtcccat ccgagacgaa gtacttctta gcgattagtt
      241 tacgtgattg attttcgtac agttctatga gaatcgcctg cttcagttcc tttgttagtt
      301 gatgatgttc atcaactaag tccgttttcg cagaaaaggt ctgcccacaa tcatagcata
      361 gaaaacgggt acgttttaat tctaaaatag tcaacttttc tttgactttc aatatctgag
      421 tacgtgtcgt ataggaaccg ttgcggataa tacgaatcga tccacagtgt tcacaatggg
      481 aaggtgagtc atccaatctc cctttaataa actgcgcttg aacgccttta tattcttttt
      541 caatcagcca ttcttcatca aaaaataaat gtttatctgt taatacgagt aattttctag
      601 tatgattatc catagagcga ctcctttggt agggtgatgt tttggtcgac taaattttac
      661 caaaagtgag tctctttttg agttgaaacg aagaaaaagt gttagtagaa aatcgccttc
      721 gattttccaa caacacaaaa tattatagaa ccctttattc taaaggattt caaggtgtct
      781 gtttgcattt attagaaaaa agaaaaagta ttggcaacag aaggtgtcac caacactaga
      841 tattatagag ccataatatg ctgtataata tctagtgtat aggcttaagt ggaggtatat
      901 atgaaaatat taactgttga agatgataat atgataagag agggaataag tgaatatctt
      961 tcagaatttg gatatactgt tattcaagct aaagatggaa gagaagcctt gtcaaaattt
     1021 aatagcgata taaatttggt tatcttagac attcagatac cttttataaa tggtttagaa
     1081 gtgttgaaag aaattagaaa gaaaagcaat ttaccaattc taatcttgac tgcatttagt
     1141 gatgaagaat ataaaattga tgcatttact aatttagtag acggatatgt agaaaagcca
     1201 ttttcattgc cggtcttaaa agctagaata gattctttaa ttaagaagaa ttatggacat
     1261 ttagagaagt ttgaatataa ggatctatcg gttaatttta atagctatac agctaaaata
     1321 aatggtgaag aaatagatgt aaatgcaaaa gaactagaag tattaaaatg tttattggat
     1381 aataatggac aagtgttaac aagaatgcaa attattgatt atgtatggaa agatagcgaa
     1441 gaaactcctt atgatcgag
//
LOCUS       DAZBTF010000181         1443 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000181 DAZBTF010000000
VERSION     DAZBTF010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.189"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..387
                     /locus_tag="ACHGNS_002990"
     CDS             <1..387
                     /locus_tag="ACHGNS_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN6938841.1"
                     /translation="DRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLI
                     ENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDT
                     YNQKFLNRSHKGFQQVTDTLVSMFTE"
     gene            810..1443
                     /locus_tag="ACHGNS_002991"
                     /pseudo
     CDS             810..1443
                     /locus_tag="ACHGNS_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          509 a          263 c          251 g          420 t
ORIGIN      
        1 gaccgaaaag aaatctgtga tgactttaag gctgtttatc aagctaactc aaaagaagaa
       61 gcgaatacct tcttatccgg catgattgag aaatggaaga aaaactatcc taaagtgacg
      121 cagtcactca tagaaaacca agacttatta actttttatg attttccacc tagcattcgt
      181 agaaccattt actcaaccaa tctaatcgag tctttcaata agcaaattaa aagatacagc
      241 cgtagaaaag agcagtttca aaatgaagaa tcactagaac gctttctagt cagcattttt
      301 gatacataca atcaaaaatt tctaaacaga agccataaag gttttcaaca ggtaaccgat
      361 acattagttt caatgtttac tgagtaacta attattttgc aggaggacaa tttatttaca
      421 caaaattatt gacgctccct gtttcaaagt tagtagagaa aatgtagtta tatatttaaa
      481 tttttaccag aaagaaatga acaccttcaa ttatctaaat ctcgaaaatg gttagatgta
      541 atcatcagga aaaacaatct taaacgaatt actattcatg gtcttagaca cacacatgct
      601 agcttgcttt tagcaagcta gtgcaaatat taaagatgtg caagagcgtt taggccactc
      661 ttctattcaa atcattatgg atctatatat tcatattaca gacaaaagaa aagaagaaac
      721 cgtagcgcaa ttcgcaaaat ataggctcta taatttatgg gactgatgaa tcaaaattga
      781 ttcttcagtc ccattgttat tttaggtata tgattcgtac attgaaagag ttgaaaaact
      841 atcttccgaa gccgtcaaaa aggaccctgc caaaagtatt gatggtggat gaatttcgtt
      901 cgcacgcttc aattgtggat aaaatgagct ttatttgtgc ggatggcgag actggaaaac
      961 tcattgatat tttgcctaca cgaaaattgt ctcgattaac acactatttt agtcgctgtg
     1021 caaatcctga agcggtggaa tatttagtga cagatatgaa cgccgcctac ttccaactga
     1081 caaaacgtgt cttaccaaat gcgaaagtcg tcattgattg ttttcatatc atcaaacatt
     1141 tgaatcaggc gttcaatgat tttcgtgtac aagaaatgaa aagattacac cagttagggc
     1201 aacgacggga agccgagaaa gtgaaaaaga actggcgctt tcttctaaaa aaccgtcaaa
     1261 aaatcaatca ctatgattac aaaacatggc gcagttttcg tgcacctaaa tacccgctac
     1321 tcactgaagc aatgatgatt gatcgattgc tggcattttc aacgccttta aaggacgcct
     1381 atactttttt ccatgagctg accgaagcct ttagagacaa ggaatcagac ttatttttta
     1441 cct
//
LOCUS       DAZBTF010000182         1422 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000182 DAZBTF010000000
VERSION     DAZBTF010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.190"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            37..447
                     /locus_tag="ACHGNS_002992"
     CDS             37..447
                     /locus_tag="ACHGNS_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFN6938842.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            461..817
                     /locus_tag="ACHGNS_002993"
     CDS             461..817
                     /locus_tag="ACHGNS_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFN6938843.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          524 a          207 c          267 g          424 t
ORIGIN      
        1 tgtagaattg gattataatt aaaaaggagt gagaaaatga taaaagtata tacttcgcct
       61 agctgtactt cctgtagaaa agcaagagca tggttgcaag cgaatcaact tgaatttgaa
      121 gagaagaata tttttgccga accgttaaca gaaagtgaga tcaaaaagat cttggcagcg
      181 actgaaggag gagttcaaga tattatttcc actcggtcga aaatttacga aaagctgggc
      241 atagatttta acgaattaac tttaaagcaa atgatcgtct tattcaaaga atatccttca
      301 ttattgagac gacctatttt gattgatgaa aaaagaatac taatcggatt taatgaagat
      361 gagatccggt catttgtccc tcgtgaaatt cgatgtgttg ccagaaatct gatctattta
      421 caaaacaata acatcttaaa ttcataggga gggagccttc gtggaagtga aagtctatgg
      481 atcagcgcac tgtgtaacaa gtctgaaggc gaaacaatgg ttagaacacc atcaattgcc
      541 ttatcaattt gttgacttag aagaacgcga tttaagtaca gaagaagcag aagaattatg
      601 cagttttgaa gaagtgcaag cagaacagct atttgcgact tggtctgagg cttttcacaa
      661 cattgcgatt gacctttcct gtgtaggaaa aaaacaactg atttttttat gcacacaaca
      721 taccaatctc ctcagacgtc ctttgattat tatcgatgaa gttctattta ttggatacaa
      781 tgagcagttg atggaacagc ttatcttaaa taaatagact ttaactgttt taatcaagtc
      841 actgagcatt tcatacagtt gtaacagtcg aacacttagc atcaagcgtg ttatgtgttc
      901 tttttttgac tttatttttc tactatagag acatgctcaa tttatgtggt caaaattcaa
      961 atctaggtgg ggatatagag ttaaaaatat attgacatgt gtaaatttac atgatatatt
     1021 catttctgtt gttttaagta gcaaagcaat aaaatagtat tttaaaaaac atataaaata
     1081 gttgttgaca tgttagaaat gacatgataa gataactgag ttgttaaaaa agaaccactt
     1141 gtgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata acaaatgata
     1201 agttatgata aatgagttgc tgtaagattt cgaaaaacga aataaaaaaa acttttaaaa
     1261 aagagttgac aattacttgt ctctctgata tgatataaaa gttgctacgg cagccaaaaa
     1321 cgaatcgggg aacactcctg atgaagtaga cctttgaaaa ctgaacaaag taagacaaac
     1381 caaatgtgta gggcgtcttg attcaattca agacaacaaa ca
//
LOCUS       DAZBTF010000183         1421 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000183 DAZBTF010000000
VERSION     DAZBTF010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.191"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(160..459)
                     /locus_tag="ACHGNS_002994"
     CDS             complement(160..459)
                     /locus_tag="ACHGNS_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFN6938844.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(465..695)
                     /locus_tag="ACHGNS_002995"
     CDS             complement(465..695)
                     /locus_tag="ACHGNS_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938845.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(954..1067)
                     /locus_tag="ACHGNS_002996"
                     /pseudo
     CDS             complement(954..1067)
                     /locus_tag="ACHGNS_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          493 a          231 c          209 g          488 t
ORIGIN      
        1 tggtagttaa ttagtgactt atttttgatt ttatagcact gatactataa aatatagata
       61 tcatcatatt acacaatctt aatactaact taaaaaatat ctcctttcat aagtatggtg
      121 ataaaatccg ttccgggcta cctttttagg tagcctactt taatctttgt atctttctgg
      181 atcaacgaaa gtatacttta tatagtcata acgccgatga tcgcttcgag cgtctagcac
      241 atcagtcacg atatcaaaca aaaaatatac gtctttcttc attctagttt tcgcagcagg
      301 gattttaaag tagttcttat tagaatagta gagattaatt aataagctat cttcgattgc
      361 taaaaagaaa acttctgaat cccacacctt ataaaaatct ttgacaaatc tattcgaagg
      421 atcaaattta aaccataatt gcgtctcatt aaaaagcata accattactc cgatctattt
      481 ttaaacttag tctctacctc taatatatat cgagttttta ttttgccttc agagaatact
      541 gtttcttttt ttgcagttac aggttgttta ttttcggaaa aagctaatat agctaaaatt
      601 gaaacatcca tctggaattt gtctttttcg ttgctttgct cataaaaatc tgcatattca
      661 tcactaatat tttttctaat aaattcttcc atcataaaga tcacctcgaa aagagtatac
      721 gaacaaacgt tctttttgta aaggtggaat ttattatact aaataaatag gtgaacaagt
      781 acttgtgcca aattatgtgc caaaaaaaat cgaatttaat aaaacacaaa caaaaagaaa
      841 tcctattatg ataacatttc ttataataac aaacacaaca gaagacgtgt aatagttagt
      901 caggaacgta caaataaccc ctgtatcctt tgcggtacag ggggttattt tgtataagta
      961 gacattgtag tttctaaact gctatgtcct aaacgtttcg atacgctaag tatatttact
     1021 ccttgataaa gtaaaataga tgcatgcgta tgtcttagtc catcaataaa cagtcgaaga
     1081 gaaaataggg aaaaattgat tactaaagaa aaacttacta ggtcgacggt gtagttgatt
     1141 ttcatacttc aatagattcg aattaaacga tttcgattgg cttataccag tcctatccaa
     1201 cgaaagatat atcgttctta actctagcgg tataacttga agctttacgt aaagccaatg
     1261 ggaatgaact ggagatgact ttttattatt tttagtaata gctgatgggt taatacttac
     1321 aactttcctt ttcaaagtac catataaaaa aacggtgggg ttgtttagcg aaataaagga
     1381 tctaagccaa tagcttagat cctttattct atgctttata t
//
LOCUS       DAZBTF010000184         1374 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000184 DAZBTF010000000
VERSION     DAZBTF010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.192"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..1372
                     /locus_tag="ACHGNS_002997"
     CDS             join(33..474,474..1372)
                     /locus_tag="ACHGNS_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFN6938846.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          576 a          177 c          239 g          382 t
ORIGIN      
        1 gactagaaat ctaattaaaa ggggtgcttt ttttgcggaa atatacattt gtttttaaga
       61 aaaaagtagt ttcagactat ttaaataacg aaggcggcta caaatatctt gcacataaat
      121 atcaaataaa tcgtacactg gttagacatt gggtaaggat ttataactat catggttggg
      181 aaggcttggt tggaggtggc aaaagctaca ctacaaaatt taaacttgat gttatagaat
      241 atatggaaac aaatggtctt tctatccaag aaactgctaa aaaatttaat atcggttcaa
      301 atagaactct aagtaaatgg atagagcaat atgaagaagg cggtgcttct tcacttgaga
      361 gccaaaaaag gggcagaaaa attagtatga attccaagct aaacattcct aaaaaactta
      421 aagatgagtc tcttgaagaa gaagttattc gtttaagagc agagaacgca tatttaaaaa
      481 agttagaaac cttgattcaa gaacaagatt tgagcaagaa gaaatcaagg ttaaaacaat
      541 ccttcagctc aaaaaagaat ttaaattgaa tctattactt tcaattgcac aattagcgaa
      601 gtcaacttat tattactggg taaaaaaatt agataagcca gataaatata gcaagataaa
      661 gcaagaaatt acagcgattg taaaagaatc aagaaactct tatggttatc gtagagtcac
      721 tttagcgtta aagatgaaag gatatacaat caatcataaa acagttagaa aattaatgtc
      781 ccaaatggga cttacctgtc aaatccgaat aaagcgatat aaatcttata aaggaacagt
      841 aggaaaaatt gccaagaatg tgttaaaacg aaatttttca gtagatacac ccaataaaaa
      901 atgggtgaca gacgtcactg aattcaagat aaaaggaaga aaaatctatc tatctcctat
      961 acttgattta tttaacggag aaataattag ttatagtata tcaactagtc caacatataa
     1021 actgattgaa gaaatgcttc aacaagcaat taaaaaaaag ggaactgagg gttcactgat
     1081 tctacattca gatcaaggat ggcagtatca gatgccacaa tatcaaaaaa aattaaaaga
     1141 gaataacatt attcaaagca tgtctagaaa aggaaattgc ttagataatt ctgtaataga
     1201 aaattttttt ggtgtgctta aatcagagtt tttttatcga gaaaaattcc gatcaattga
     1261 gatatttcaa agtaaattaa atgagtatat taggtggtat aataacaaaa ggataaaact
     1321 aaagttaaat ggactatctc cagtagaata tcggaaacag tccattaaat agtt
//
LOCUS       DAZBTF010000185         1368 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000185 DAZBTF010000000
VERSION     DAZBTF010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.193"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1368
                     /locus_tag="ACHGNS_002998"
     CDS             82..>1368
                     /locus_tag="ACHGNS_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFN6938847.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLIN"
BASE COUNT          467 a          263 c          257 g          381 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taatcaat
//
LOCUS       DAZBTF010000186         1364 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000186 DAZBTF010000000
VERSION     DAZBTF010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.194"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACHGNS_002999"
     CDS             complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACHGNS_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="HFN6938848.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          393 a          242 c          216 g          513 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcagatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgcc
//
LOCUS       DAZBTF010000187         1355 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000187 DAZBTF010000000
VERSION     DAZBTF010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.195"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1355
                     /locus_tag="ACHGNS_003000"
     rRNA            <1..1355
                     /locus_tag="ACHGNS_003000"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DAZBTF010000188         1324 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000188 DAZBTF010000000
VERSION     DAZBTF010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.196"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..820
                     /locus_tag="ACHGNS_003001"
     CDS             278..820
                     /locus_tag="ACHGNS_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938849.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            976..>1324
                     /locus_tag="ACHGNS_003002"
     CDS             976..>1324
                     /locus_tag="ACHGNS_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFN6938850.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          533 a          157 c          248 g          386 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcaaat tgatgaagca gatcccaaag ggctgcttca tcaatttttt
      121 cgtaagtgtc cgtacactta ctgtgcttgc tcatatatta atcgatcata tactaatcgt
      181 gtaagaacaa ctttaagcat aatacttata ttgttatacc atagtttgga aaaataaaaa
      241 attatatttc atcaacgttt cataaaaggt gtgtagaatg ttattgaaag tagctataaa
      301 atgctattgt gtaaaaaata aaggaggaat aatttatgaa aaaatagtac caagaggagc
      361 atgcctatta cttgcattat gtttgagctt aagcgcagtt gggtgtcaaa aaagtaatga
      421 aaacaataaa ataaaagaag gacaatccat aagttctgaa gaggaaggca tgtcaattaa
      481 tgcaaatggc aaaaatgaaa cttttaagcc atcagattat acattggagg caaagaaaga
      541 atatgtttat gaatatttag ggcttaaatt taaactctca gacaaattta gaaattatat
      601 agctgataaa aagatagcga tgttagatga tcaaagtcct atagataaag aactaaagta
      661 tgctattttg acatttgaga aaatgacaga agaacaaaag aatgcagtta tagaaaagat
      721 gggcgatgga tataaaaatt ggcaaaatga gcttgaaaga attgggacta ttggtatatt
      781 tgaaaaaaat acatcagaag aaaaaaatct aaaattataa aatgtgatac tcatactaaa
      841 ataggagttt caagtgatgg aaaatacgat tgctatttaa gcacaaatag cggagctgaa
      901 agtaatcttc tggatgaatt aaaaagaact gagattcaaa ttatagataa aaaagaacgt
      961 cctaaaaatg gttttgtgct atcagaaaaa actgatttag aaaatacaga agcatttaac
     1021 aaagaatcgg ttaaagattt acgtaaacta ccaacaaagg acataaatgg caaggatttt
     1081 acaagtaaag attttgagaa gtatgatctt actatggtaa atgtatttgc aacttggtgt
     1141 acagcttgtg taaaggaaat tcctgatttg gttgaggttc agaatgaaat gaaaagcaag
     1201 ggtgtaaata tcgttggagt tgttacagat cctgttgatg ataacggtga aaacaaggaa
     1261 gcaattgaaa agtcaaagtt aatacatgaa aagacaaagg cttcatatcc tttcttaatg
     1321 ccag
//
LOCUS       DAZBTF010000189         1305 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000189 DAZBTF010000000
VERSION     DAZBTF010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.197"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(296..903)
                     /locus_tag="ACHGNS_003003"
                     /pseudo
     CDS             complement(296..903)
                     /locus_tag="ACHGNS_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
BASE COUNT          441 a          232 c          217 g          415 t
ORIGIN      
        1 agagattggc gcagcgaatc cttgataatg agctcggatg tgctaacgct agcgaggcag
       61 tctaacttta actgttagct cctgccgaag atcgtcacat ccgaggaggc tcattgtcaa
      121 ggacaatcgg aatagcatac ggaaagaagt tctgcacctt ataaaatttg tcaaaaaaac
      181 tgttgccatt ccatctatga atataaatag agtgtatctt ctttgataaa tgtcagaaaa
      241 agtgtgacaa ataaccatca gtaaaattac ttgtcatact attgataagg agtatttaat
      301 tgaagagaat gattaggaga aaggatctcc gtacttttta tagtaatcat agaatccaag
      361 tttgtataga cgaatctttt tttctcttat ttcttctgtt tcaaaatgga ctgcttgaag
      421 aatcaagtct gtgaattcta taggagctgt tccattcgca gtaatcaaat tacgatcttt
      481 cacagcttgt tgttctataa aatcttcttt attctgataa ttttccgatt ctaaccaagg
      541 aaattgcgca tttccggtgt gtttgtaacc ttgcaataat ccgctctgag ctaaataatc
      601 aactgcccca caaatagcag ctactggtac gtctgttttt aacaatttat cgattatttg
      661 atataaatga tgatttttta tgtcccatga attgccaccg atcataataa aaaggtcaac
      721 tttttcaggt atttgttcga ttaagtaatc gatttttgtt tttaatcctc caatggaagt
      781 gacctctgat tggatagaag cagttttgtt ttgccatccc tctttttggt ttagtgtgct
      841 agtaatataa gcaccttccc aatcggcata ttcctctaat agaaacgtaa tcgcagtttt
      901 cattttcaag ctctccttta ctatctatga caaggacaac agttttaaca tacagacatt
      961 cattaaattt ggatagcgac agcgaaaaca acaattctta agttttagac agagtataca
     1021 gacagtcaac ttaattaaat acgaactaaa taaaaatgac aggtaatcat ttttgtttac
     1081 cagcattata acattttgca tacatggatg acaaaagaac caattcccac ttgcttgagt
     1141 acaatcttaa aagaaactgg aaggcaaaaa gtaattatag aaacgaaaaa taaatcatat
     1201 cgggcactca gacatgaaaa caaccctaga aatatataac tagtccaatg aaaaaaaagc
     1261 gaatgccgta gaaaacaagg attctgcgta cattcatatc tcatt
//
LOCUS       DAZBTF010000190         1276 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000190 DAZBTF010000000
VERSION     DAZBTF010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.198"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..87
                     /locus_tag="ACHGNS_003004"
     CDS             <1..87
                     /locus_tag="ACHGNS_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFN6938851.1"
                     /translation="FKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            92..1039
                     /locus_tag="ACHGNS_003005"
     CDS             92..1039
                     /locus_tag="ACHGNS_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFN6938852.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          411 a          186 c          270 g          409 t
ORIGIN      
        1 tttaaatggt tagaattttt gtttcctaaa tttttagaag attcttcgga gcttcaagat
       61 atctataatt atttatatcg gaggtaaaag gatgagctgt tatcttatca ccggtggggc
      121 tggcttcatt ggttctacct tagccaatca tttaggagaa gaacatgtgg taatcgtcgt
      181 cgatgactta tcaatgggaa aagtagaaaa tcttgatatg acgaaaaata tcacttttat
      241 tgagggagat gtcgctgaga aatccttgat ggaagagatc atgaaagcta ataaatttga
      301 ttatatcttt catttagcag cagttgctag tgtggcagat tcagttgccc gaccagtaga
      361 aacacatcgt gtgaattttg aaagtgtatt aatgatttta gaattgatcc gcaagtatca
      421 acctgattta aaacggatcg tgtttagttc ttcagcggct gtttatgggg atgaaccgac
      481 tttaccaaaa aaagaagagt ccgtgattcg cccattgacg ccttatgcgg ttgataaatt
      541 cgctgcagaa caatatgtgc tagattattg tcatttatat gatgtacccg gtagcgcagt
      601 tcgttttttc aatgtctatg gtccaaatca aaatccgaat tctccataca gtggtgtgat
      661 ttctattctc gtagatcgct ataagaaaca aatcgtagga gaagcaactt catttacatt
      721 atttggtgat ggaagccagt ctagagactt tgtttatata gatgatgtaa tccaagcatt
      781 acttttagtt gcgaatgagg aagctgcact ggggaaacaa ttcaatgttg gaacgggtaa
      841 agcaaccaca cttttggaat tgattcatgc aatcaatgaa attctcggtg tagaattgac
      901 acttgaatac aaagaagagc gttcgggtga tatccatgac tctctagcag atattactaa
      961 gatccagtca attggctatc aaccgaaata tggtatttta agtggaatga ctaattattt
     1021 gaaaactgaa atcaattaaa acatactaag ataagattag agggtaaatt caggctcttt
     1081 gtcaataagg actgatgagt tttgtaaaat caaatctggg atagaagggc tctttgtcaa
     1141 atgatgttgg taaagagaaa tcttagaata atttggacag aacagaaaga agaaaacctc
     1201 tttctgttct gtttatttgg ttaaaaatgt tttgccatga cgaatcataa ttcgtagttt
     1261 gaaattgtaa aagttg
//
LOCUS       DAZBTF010000191         1195 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000191 DAZBTF010000000
VERSION     DAZBTF010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.199"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1076
                     /locus_tag="ACHGNS_003006"
     CDS             <1..1076
                     /locus_tag="ACHGNS_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFN6938853.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DAZBTF010000192         1069 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000192 DAZBTF010000000
VERSION     DAZBTF010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.200"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..969
                     /locus_tag="ACHGNS_003007"
     CDS             <1..969
                     /locus_tag="ACHGNS_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFN6938854.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          368 a          185 c          214 g          302 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccat
//
LOCUS       DAZBTF010000193         1063 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000193 DAZBTF010000000
VERSION     DAZBTF010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.201"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..993)
                     /locus_tag="ACHGNS_003008"
     CDS             complement(37..993)
                     /locus_tag="ACHGNS_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938855.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFNFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          178 c          159 g          380 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt tgaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgtc aac
//
LOCUS       DAZBTF010000194          993 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000194 DAZBTF010000000
VERSION     DAZBTF010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.202"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            265..957
                     /locus_tag="ACHGNS_003009"
     CDS             265..957
                     /locus_tag="ACHGNS_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFN6938856.1"
                     /translation="MRKKIIINAGVIIGLMLVIFSGKQYADNILLWGGEDNIEKINNN
                     LTILDTALENKEQKISILNSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKT
                     NLENQLSAKDSELQGKQNEINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQST
                     INSLNQQINQMLSEKNNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTAN
                     KAQQIVNEHTIQ"
BASE COUNT          453 a          113 c          146 g          281 t
ORIGIN      
        1 aattattgac acaacctatt ctggaaactt acaaataaaa gcttattctt cagccacctt
       61 cgggtggtta ttttttataa aaaaatgtaa aaaaatattg atacataaat aaattagttg
      121 tagtaaatag ttttagaatt gaatttctct atccttttta aataattttt tagacaaaaa
      181 acagaaagtg tctaaagaaa gcttgacgaa gaaaataatt tttaataact tatttaagga
      241 aattttttga aggagaagat aaaagtgaga aaaaagatta ttattaatgc tggagtaatt
      301 ataggattga tgttagtaat tttctccgga aagcaatatg ctgataatat tttattatgg
      361 ggaggggaag ataatattga aaagatcaac aacaatttaa ctatattaga cacagctcta
      421 gaaaataaag agcaaaaaat tagtatttta aattcacaat tgtcttcaaa caaccaaact
      481 cttgaacaac taagaactaa tattgaagtt tccaaacaaa aagtggcatc tttagaaaac
      541 gacaagaatc aattagtatc agagaaaact aatttagaaa atcaattgtc agcaaaagac
      601 tctgaattgc aaggaaaaca aaatgaaatt aatgcgaaaa tagacgaaat taaccaaaaa
      661 gtagctgaaa taaatcgatt aaatagcgat tggaataatc gtttacaaaa tgaaaagaac
      721 aaatttaatg aagaacaatc tactattaat agtttaaacc agcaaattaa tcaaatgttg
      781 agtgaaaaaa ataacattca aaatcaactg caagctactc agcaaaagct agaagaagaa
      841 aagtcaaaaa atagtagttt gaataattac attgaaaagc ttcaaaaagc taaaaatgat
      901 gtagaggata ccgcaaataa agcacaacaa attgtaaatg aacatacaat ccaatagata
      961 tagttttaat ttaacaaatg gtatgtttgg aga
//
LOCUS       DAZBTF010000195          986 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000195 DAZBTF010000000
VERSION     DAZBTF010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.203"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>986
                     /locus_tag="ACHGNS_003010"
     CDS             <1..>986
                     /locus_tag="ACHGNS_003010"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFN6938857.1"
                     /translation="LEPGDFIMSGAGTIGRISRVPKQIKPGVFNQALIRLRINKEITD
                     SEYFIQFIRADFMQRKLTGANPGSAITNLVPMSEVKKWIVQFPILEEQKKIGNFFKQL
                     DDTIALQQRKLDLLKETKKGFLQKMFPKNGAKVPEIRFPGFTEDWEQRKFKEFSKKTG
                     KKNTKDLDFPAYSVSNKAGLISQTEQFDGSRLDDLEKTNYKFVEPNEFAYNPARVNVG
                     SIAFNNLGMTVIVSSLYVVVKMSEDLDNEFILQFIKSPTFIKEVKRNTEGSVREYLFY
                     ENFANIKFPFTRNKEEQQKIGAFFKQLDDTIALHQRKLDLLKETKKGFLQKM"
BASE COUNT          375 a          159 c          189 g          263 t
ORIGIN      
        1 ttagaaccag gcgattttat catgagtggc gctggaacaa ttgggagaat ttcaagagta
       61 ccaaagcaaa taaaaccggg tgtattcaat caggctttga ttagacttag aataaataaa
      121 gaaatcacag attcagaata ttttattcaa tttattcgtg cagattttat gcaaagaaaa
      181 ctcacaggag ctaatccagg ttcagcaatt acaaatctcg taccaatgtc agaagtgaaa
      241 aaatggatag tacaatttcc aatattagaa gaacaaaaga aaattgggaa cttcttcaaa
      301 caactggacg acactatcgc tcttcaacaa cgtaagctag atttattaaa ggaaacgaaa
      361 aaaggattct tacaaaaaat gttccctaaa aacggagcaa aagtgccaga aattcggttt
      421 cctggattta cggaagattg ggaacagcgt aagttcaaag agtttagtaa gaagactgga
      481 aaaaagaaca cgaaagattt agacttccct gcctattcgg taagtaataa agcaggttta
      541 atttctcaaa cagaacagtt tgatggtagt aggttggatg atttagaaaa aacaaattat
      601 aaattcgttg aaccaaatga attcgcctat aatccagcaa gagtaaacgt aggatcaatt
      661 gcatttaaca atcttggtat gacggtcatt gttagctctc tttatgtagt tgtgaaaatg
      721 agcgaggact tagataatga gttcatactt cagtttatca agtcaccaac atttatcaaa
      781 gaggtcaagc gcaacactga aggaagcgta cgagaatact tattctatga gaatttcgct
      841 aatatcaagt ttccttttac taggaataaa gaggaacaac aaaaaatcgg cgctttcttc
      901 aaacaactcg acgacacgat cgctcttcat caacgtaagt tagatttgct gaaggaaacg
      961 aaaaaaggct tcttacaaaa gatgtt
//
LOCUS       DAZBTF010000196          973 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000196 DAZBTF010000000
VERSION     DAZBTF010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.204"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..532
                     /locus_tag="ACHGNS_003011"
     CDS             <1..532
                     /locus_tag="ACHGNS_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFN6938858.1"
                     /translation="IVIESMKDGLHIYDNPVGVLTNNPSFDYQLFNLNNYRVLSSETP
                     KNNFSNQISLNAYSRGMGGIGLPGDLSSVSRFVKATFTKLNSVSGDSESESISQFFHI
                     LGSVEQQKGLCDVGDGKYEYTIYSSCCNVDKGIYYYRTYEDSQITAIDMNKEDLDSHK
                     LISYPIIEKQQIKYIN"
BASE COUNT          336 a          125 c          157 g          355 t
ORIGIN      
        1 aattgtcatt gaaagtatga aagatggact tcatatatat gataaccctg tgggcgttct
       61 taccaataat ccttcatttg actatcaatt atttaattta aacaattatc gtgtcttatc
      121 gagtgaaact cctaaaaata atttttcaaa tcaaataagt ttgaatgcct atagccgcgg
      181 tatgggaggg ataggcttgc ctggagattt atcctcagta tctcgttttg ttaaagcgac
      241 ttttacgaag ctgaattctg tatctggaga ttcagagtca gaaagtatta gtcaattttt
      301 ccatatctta ggttcagtag aacaacaaaa aggtttgtgt gatgttggtg atggaaaata
      361 tgaatataca atttattctt cttgttgcaa tgttgacaaa ggaatctatt attatcgaac
      421 atatgaagac agtcaaatta ctgcaattga tatgaataaa gaagacttag atagtcataa
      481 gttaattagt tatccaatta tagaaaaaca acaaattaaa tatataaatt agttaatgtg
      541 ttgtgattga ttatttaata tagatataat acaaaaaggc aaagatattt tcaaaacatt
      601 ttttcttgat gtgatatcct tgtctttttt gtaatgtttt taacttattg cttttgcaaa
      661 atttatttac ctgttttctg gatcaacgat agtgtatatt atgctatcct tgtttggatt
      721 ttaggacgtt taaagaggca cttttttaca taacttaatc catagacctt ttaagcatgc
      781 tgtgagataa taataaagaa gagtttaaag cgcaccccaa accacttccc cataagtgta
      841 ttacgcttta aactctttta tatttgaatt cactaagaag cataccatat ttttgaaaaa
      901 aagtgagaaa aaaggcttat aattaggatt tagagtaatt aattagatgt ttattttata
      961 ggactgatac tat
//
LOCUS       DAZBTF010000197          943 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000197 DAZBTF010000000
VERSION     DAZBTF010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.205"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>943
                     /locus_tag="ACHGNS_003012"
     rRNA            <1..>943
                     /locus_tag="ACHGNS_003012"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DAZBTF010000198          920 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000198 DAZBTF010000000
VERSION     DAZBTF010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.206"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>920
                     /locus_tag="ACHGNS_003013"
     CDS             <1..>920
                     /locus_tag="ACHGNS_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFN6938859.1"
                     /translation="KSCQRTFVADTSVAEKHCFISQKVRWSVIARLKENTSMTEIARQ
                     KNISTSSVYRVMKRFYRPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHALL
                     DIVENRRLPYLERYFSRFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIV
                     QHIGRTFLNHRVKETTVRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASF
                     KDYLTESEIADRLLAACPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYT
                     TTLRTFKKFLPQIQNALHYSYSNGPLECLN"
BASE COUNT          279 a          186 c          183 g          272 t
ORIGIN      
        1 taagtcttgc caaagaacat tcgtagcgga tacctctgtt gctgaaaaac actgtttcat
       61 tagtcaaaaa gttcgttggt ctgtcattgc tcgcttaaaa gagaatactt ccatgacgga
      121 aatcgctcgt cagaaaaata tttccacttc ttcggtctat cgtgtcatga agcgattcta
      181 ccgcccattg aatcctttca aacagacttt gccaaaggtc ttatgctttg atgaattcaa
      241 atcggttcga agtgttgcca gtgcaatgag ttttatcatg atggacggac aaagccatgc
      301 attattggat attgtcgaaa atcgacgctt gccttattta gaacgctatt tttctcggtt
      361 ttcattagcg actcgtgaag ccgttcaatt gattgttatt gatatgtatg cgccttatgt
      421 ttcgttggtg aaaaagcttt tcccgaatgc tcagttgatc atcgatcgtt tccacatcgt
      481 ccaacatatt ggcagaactt tcttgaatca tcgagtgaaa gaaaccacgg tacgacttaa
      541 aggaaattcc cactctcaac gagggttagg gaaaaaattg aagcgctatt ggaaactcct
      601 acaaaaagaa gaagcaaagt taaactatga aaaacgggtt tggcgtgcca gttttaaaga
      661 ttatttgact gaaagtgaga ttgccgatcg actgttagct gcttgtccca acttacgcca
      721 aggctatcag ttataccaag acattcttta tgcggtaaag aaaagagatc agtcactatt
      781 tgaggattgt ttaactagag acattagcgg tctgccagaa acctatacaa ccactttacg
      841 aacgtttaaa aagtttctgc cacagatcca aaatgcgctt cactacagct attcaaacgg
      901 accattggaa tgcttaaaca
//
LOCUS       DAZBTF010000199          917 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000199 DAZBTF010000000
VERSION     DAZBTF010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.207"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            88..>917
                     /locus_tag="ACHGNS_003014"
     CDS             88..>917
                     /locus_tag="ACHGNS_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFN6938860.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT          318 a          136 c          158 g          305 t
ORIGIN      
        1 taaatagata tttagggtag aaaaaaagaa agtccattct gtaaaattaa agttaccaca
       61 accgaaattt tataggagga ctttctcatg cccaatttta ctacagaaat tatggaaaca
      121 ctaatcaata aaggtgattt agatgaatta tttcgatgtc atttagaatt agctgttaat
      181 tcattattgc aggctgaatt aacagctttt cttgattatg aaaaatatga tcgtgctggg
      241 ttcaattcag gtaattcccg taatgggaat tattcacgtt cattcagaac agagtatgga
      301 gaattaaatt tggtaattcc tagagataga aatggtaagt tttctcaaca aactttacct
      361 gcttataaaa gaaccaatga ctctttagaa accactatta ttcagctatt tcaaaaagga
      421 attactatgt ctgaaatctc tgaattgatt gaaaaaatgt acgggcatca ctatacgcca
      481 caaaccattt caaatatgac taaaatagtt tctgaagata ttattgcctt taaagaaagg
      541 tccttagaat ccagatactc tgtcattttt atggatgcta ctcacattcc tttaaaaaga
      601 caaactgttt caaaagaagc agtttatatt gtaattggca ttcgattaga tggaaccaaa
      661 gaggttcttg ggtttagtct tgctccaact gaatctgctt atgtttggaa agagattctt
      721 caagacctaa aagaccgtgg tttaaaagag gttttattag tcgtaactga tggtttaagt
      781 ggcattaacg atagtattca tagtgtttat ccaaatgctc aatttcaaca atgttgtgtc
      841 catatatcta gaaatatcgc tcataaggtt cgtgttagtg atcgacaaga aatctgtagc
      901 gatttcaaat tagttta
//
LOCUS       DAZBTF010000200          843 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000200 DAZBTF010000000
VERSION     DAZBTF010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.208"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>843)
                     /locus_tag="ACHGNS_003015"
     rRNA            complement(<1..>843)
                     /locus_tag="ACHGNS_003015"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          184 a          259 c          187 g          213 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gct
//
LOCUS       DAZBTF010000201          812 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000201 DAZBTF010000000
VERSION     DAZBTF010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.209"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..613)
                     /locus_tag="ACHGNS_003016"
     CDS             complement(<1..613)
                     /locus_tag="ACHGNS_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HFN6938861.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQSRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL"
BASE COUNT          245 a          158 c          150 g          259 t
ORIGIN      
        1 gcaggaagta ttgagttagt ttgtaaagtc tgcgatcttc aaggatcgta aatagctgat
       61 gggttgtccc atcaacacaa ataaaactca tggaaccaga aacagacttc attgatttga
      121 actcatccat acacaacacc gtcggaagga agttcatacg tgactggtaa tgcttcgttg
      181 cttcacgcaa gatccgtgtc accgtcccat ccgagacgaa gtacttctta gcgattagtt
      241 tacgtgattg attttcgtac agttccatga gaattgcctg cttcagttcc tttgttagtt
      301 gatgatgttc atcaactaag tccgttttcg cagaaaaggt ccttccacaa tcatggcaga
      361 gaaaacgggt acgttttaat tctaaaatag ttaacttttc tttgactttc aatatctgag
      421 tgcgtgtcgt ataggaaccg ttgcggataa tacgaaccga tcgacagtgt tcacaatagg
      481 aaggagagtc atccaatttt cctttaataa actgcgcttg aacgccttta tattcttttt
      541 caatcagcca ttcttcatca aaaaataaat gtttatctgt taatccgagt aattttctag
      601 tatgattatc cattgagaga ctcctttggt aggatgacgt tttggtcgac taaattttac
      661 caaaagtgag tctctttttg agttgaaacg aagaaaaagt gttagtagaa aatcgccttc
      721 gattttccac caacacaaaa tattatagaa gcaaaatcaa ttctacctta gatgttctta
      781 tatcactcta tcagtcctat ttgacaaaga gc
//
LOCUS       DAZBTF010000202          786 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000202 DAZBTF010000000
VERSION     DAZBTF010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.210"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>786
                     /locus_tag="ACHGNS_003017"
     CDS             <1..>786
                     /locus_tag="ACHGNS_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFN6938862.1"
                     /translation="KKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPLILLG
                     FIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIFLIIK
                     LIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLS
                     FGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQA
                     PWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAI"
BASE COUNT          254 a          110 c          108 g          314 t
ORIGIN      
        1 ccaaaaaaca tgttactagc tcctttttcg ctattatata tacaacatct tcttttagga
       61 gtgtagaaat atttcttcga ggggcaatgg gggaattact cgcaatgtca atacttcctc
      121 ttattttact cgggtttata aagctttacg actcaaaaaa agaatcttgg gttatgttgg
      181 cgattagcat gactttatta atttacactc atgttctttc tgtagctatg actatggtaa
      241 tgattataat tgctttaatt attcaatttt atcggaaaaa gaaaatagaa atcttcttaa
      301 ttattaaatt aataaaagct gctatagtta ctttacttct ttcactagcg tttataggtc
      361 ctatgattga gcagacattg tatcaagatc tgaatagacc ttatgtggat atattacaaa
      421 aaagagcaat ttatctgggt aaagagtttt tgattggaag tatagattca gaattactat
      481 catttggtat tggcttaact ttgcttattt gtttaattat actaacattt aactttaaaa
      541 aattaggaat cttatctaaa atcacttata taatgggtat aatatctatt ctactttgta
      601 caaaaatatt tccatggttt gccttacaaa atactccttt aaatattatt caagctcctt
      661 ggcgttttat gatattttca acattgttca tatctttttc agtatctcta tttatttctc
      721 cacagttgga aaaattttct tatacaagga gaagaaattt tatactatta ttaatattag
      781 ctattt
//
LOCUS       DAZBTF010000203          696 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000203 DAZBTF010000000
VERSION     DAZBTF010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.211"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..629)
                     /locus_tag="ACHGNS_003018"
     CDS             complement(54..629)
                     /locus_tag="ACHGNS_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFN6938863.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          230 a          151 c          124 g          191 t
ORIGIN      
        1 aatgaaaaaa cagagccaga ttagcattat tttgctaatc cagctctgtt tattcagcct
       61 atttttcttt ttgaatatat caagcgttgg ggcaacatag tgatgactaa tgaaacaacg
      121 acgatagaca acaagcgatc aaggtagtct gtacccgcct gtaccaagat cacacttgtt
      181 gtctgactta aacctaaccc atgtaatagc tgaacgatca tactggatcc tgaggaggtg
      241 atcccgccaa ataactgaac ggtaatgatc gaagaaacga tcgttccagg taccgacaaa
      301 atcaaagcga gtaataacag tttcaagctg tttttatctt tgccgagata tcttggcaaa
      361 atcaatccag caagcaagcc tgtgagaatt tggattggcg aataatacag agaaaaaata
      421 tctgtagtca ctccacttaa aataccgctg gacaatcctg tcagcattcc aggtacaggc
      481 ccaagcaatg caccagcaaa aatcgttccg atagaatcga gatagattgg caaacgaagc
      541 cataaagcaa tcgttcctcc aatataattg atcccaatac acaaagccat cactgtaagt
      601 acgcgagttg ccattttgtt tttattcatt ttgttagccc atctttaacc tttccaaatc
      661 tttcataatc gtttcttctt gtgcatttaa aagaac
//
LOCUS       DAZBTF010000204          691 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000204 DAZBTF010000000
VERSION     DAZBTF010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.212"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(374..>691)
                     /locus_tag="ACHGNS_003019"
     CDS             complement(374..>691)
                     /locus_tag="ACHGNS_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN6938864.1"
                     /translation="ANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYS
                     TNLIEGFNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLE
                     SMF"
BASE COUNT          234 a           94 c           95 g          268 t
ORIGIN      
        1 tcaagggatt tagtttatga ttttatatca tttcagtaac tcagaatatt ccgacttttt
       61 tgtacaaaaa aattaacctt acttatatga agattctaag ctcttattta aattttagac
      121 gggcagttat ccgtagaagt tcttagaata gctaatttac ataattaaat tatatgaaat
      181 ttaagtttga acaatatact aagaactccc cttactataa agtgaaataa attagcatat
      241 gaataaccgt gctaccaagc ttttcattaa taaaatattt ttattaatat tttataactt
      301 tacgcaagat cataattttg tcgggagcgt caataatttt gtgtaaatgg aaaagcccat
      361 tctgttgatc aagttaaaac attgattcta atgtatcatg tatttcttta aaacctttat
      421 gaactcggca tagaaacttt tgattatagt tgttgaattg agatactaga aatctctcca
      481 gagattcttc attaggaaat tgctcttttc ttttggtata tttctttaat tgtttgttaa
      541 aaccttcaat caaattagtt gaataaatag ttcttctgat tgacggaggg aaattataaa
      601 aggttaatat agcaggattc atgagcaatt taaccactcg tggatactgc tttttccatt
      661 tatctatcat aaaccttatt tgattatttg c
//
LOCUS       DAZBTF010000205          681 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000205 DAZBTF010000000
VERSION     DAZBTF010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.213"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..448)
                     /locus_tag="ACHGNS_003020"
     CDS             complement(<1..448)
                     /locus_tag="ACHGNS_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFN6938865.1"
                     /translation="MNKDITFPVMDVRLVPITKIQANDYNPNRVASPEMNLLELSIIE
                     DGFTQPLVCYYKEKEEKYILVDVLHRYRVAKERLNLKVVPVTVIDKPIEERMASTIRH
                     NRARGTHEIRGMEIVIQRLVKNGWSDKRISKELGMDIEEIFRFKQVS"
BASE COUNT          202 a          137 c          103 g          239 t
ORIGIN      
        1 cagacacttg cttaaagcga aaaatttctt caatgtccat gcctaattct ttggatattc
       61 tcttgtctga ccatccattt tttactaatc tttggatgac aatttccatt cctcttattt
      121 catgggtccc tcttgcacga ttgtgtcgaa ttgttgaagc catacgttct tctatgggtt
      181 tatcaataac tgttaccggt acaactttta agttgagtct ttccttagca acacgatacc
      241 tatgaagcac atcaaccaag atatacttct cttctttttc cttgtaataa caaactaatg
      301 gttgagtaaa gccatcttca atgatagaaa gctccaataa gttcatttcc ggagacgcta
      361 ctcgattggg attataatcg ttcgcctgta ttttggtaat tggaacaagc cgaacgtcca
      421 tcacagggaa agttatgtct ttattcatac gcttgtatac cctactttct taaaaagtag
      481 ggtatactca agaatccgat taattatctt ctcaatttgg ataatatatc tcatcagtct
      541 tcttgtcact ttatcaacat gattttgctg caaaatggga taccgacagt actggactgg
      601 taatcaggat aaaagcaaaa ataattaatt tgtatcctaa aaaatccata aaaattctct
      661 cacattttcg ggtattaatt c
//
LOCUS       DAZBTF010000206          670 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000206 DAZBTF010000000
VERSION     DAZBTF010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.214"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..>670
                     /locus_tag="ACHGNS_003021"
     CDS             64..>670
                     /locus_tag="ACHGNS_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6938866.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSDTFSLDKDYELLNPIIKNIFTKYYSKDFEIYLNEILANNINPLIEEYANQRH
                     SISKELFFKRANSKLQDKIRNGDFKYGEKERMIDSLKQLLDE"
BASE COUNT          279 a          101 c           99 g          191 t
ORIGIN      
        1 ttttaaatac ttttatttta taaatatgat atactatctt taaaaataaa aaggagaaaa
       61 aagatgaagc caaatccttc agaagcagga aaacgaatca aacaattacg cctttcatgt
      121 ggttttacta tggaagaact tggaaggaaa atagataatt caccacgtgc gaccatatcc
      181 aactgggaac gtgggaccaa tttaccaaat ccacaaaagc tgaaattatt atcaactatc
      241 actaattcaa caattgactg gattaaatgg ggaactttag aagaatatat aacttcatac
      301 cttatcgata tagggtatga gctatatatt aaggacttcc cagaaatacc tcacaaagtc
      361 tttaaagaca ttcaagaaag atatagtgat acgttttcgc tagataaaga ctatgagtta
      421 ttgaatccta taataaaaaa tatatttaca aaatattact caaaagattt tgaaatttat
      481 ctaaatgaga tacttgctaa taatatcaat cctctgattg aagagtacgc taaccaaaga
      541 cactctatct ctaaagagct atttttcaaa agagccaaca gtaaacttca agacaagata
      601 agaaatggtg atttcaaata cggtgaaaaa gaaagaatga ttgattcttt aaaacagcta
      661 ttagacgaaa
//
LOCUS       DAZBTF010000207          660 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000207 DAZBTF010000000
VERSION     DAZBTF010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.215"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            46..>660
                     /locus_tag="ACHGNS_003022"
     CDS             46..>660
                     /locus_tag="ACHGNS_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN6938867.1"
                     /translation="MKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRTPI
                     REALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQKK
                     RILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERFRW
                     LRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLN"
BASE COUNT          266 a          101 c          109 g          184 t
ORIGIN      
        1 taaaatatca gttaaaataa aagttaagga gcattgtgta tgacaattaa aactagtctc
       61 caaaatcaag cttaccaatc tattcgccga caaattatct atgctgactt agaacctgga
      121 aaaaaagtat cagaaaaaaa attagaagaa acactttcta ttggtcgtac accaatacgt
      181 gaagcactga ttcaactccg acaacaaggt cttgttgata caattcctca aagcggaaat
      241 tatatctcat taattgattt agattcggcc aaaaatgctc gatttgtcag agaacatttg
      301 gaacgacaaa ttatgttaga gtgttgtgcc aaaatgaata accaaaaaaa acggatttta
      361 gaaactataa tttcagaaca agaaaaagct gttagtcaaa aaaatgctcg tagttttttt
      421 cataatgata atttattcca taaagcttgt tttgaaattg cagagcgaga agaagtatgg
      481 caatggttag aaaataacaa tacacactta gaacgatttc gatggctacg agtaaaaacg
      541 gaaggattaa agtgggaaaa aattatggaa caacattatc aactatttga ggcattaaca
      601 aaacaagatc cagaagaagc taactttcta acatcgttgc atcttcattt aatgcttaat
//
LOCUS       DAZBTF010000208          563 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000208 DAZBTF010000000
VERSION     DAZBTF010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.216"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..163
                     /locus_tag="ACHGNS_003023"
     CDS             <1..163
                     /locus_tag="ACHGNS_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFN6938868.1"
                     /translation="ISFEENIVKEMPESANDMYEKELKYFFENVVTGKENWNDLYHAN
                     EVLKIAEEK"
     gene            164..>563
                     /locus_tag="ACHGNS_003024"
     CDS             164..>563
                     /locus_tag="ACHGNS_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFN6938869.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKN"
BASE COUNT          219 a           64 c          107 g          173 t
ORIGIN      
        1 aataagtttt gaagaaaaca ttgttaaaga aatgcctgaa tctgctaatg atatgtatga
       61 gaaggagttg aaatatttct ttgaaaacgt agtaacaggc aaagaaaact ggaatgattt
      121 atatcatgca aacgaagtat tgaaaatagc tgaggagaaa taaatggaaa ttttatttac
      181 tatttgtggt agagctgggt ctaaggggtt taaaaataaa aatttgaaat atttggcagg
      241 aaagccccta gtccatttcg ctttagcaat gatagatatc tatcaacaaa aacatcctga
      301 acataattgc acgacttgtt tgaatacgga cagtaatgag ttagtagaat tgacgactaa
      361 gttaaatccg tctgtgaaaa ttgtagaacg taaacctgaa ttagctacag atactgtagg
      421 gaaaattgaa gtgatacgtg attcattctt acaactagaa agagaagata atatatttga
      481 tttaattata gatttagatt tgacttcacc tttacgtgaa gtttctaatc ttgaagaatt
      541 aatagatgaa catctaaaaa att
//
LOCUS       DAZBTF010000209          535 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000209 DAZBTF010000000
VERSION     DAZBTF010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.218"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>535
                     /locus_tag="ACHGNS_003025"
     CDS             <1..>535
                     /locus_tag="ACHGNS_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFN6938870.1"
                     /translation="QDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHTTIYRWVQEY
                     GKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWLRKKRDTQA
                     AYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLNNLIEQDH
                     RPVKRRNKFYRSLRTAST"
BASE COUNT          184 a           95 c          104 g          152 t
ORIGIN      
        1 gcaggatgtg attattgtag ccgtgggcta ctatcttcgt tataacctta gctatcgtga
       61 agttcaagaa atcttatatg atcgtggcat taacgtttct catacgacga tttatcgttg
      121 ggtgcaagaa tatggcaaac tactctatca aatttggaaa aagaaaaata aaaaatcctt
      181 ttattcatgg aaaatggatg aaacgtacat caaaattaaa ggaaaatggc attatttgta
      241 tcgagccatc gatgcagatg gtttaacctt ggatatttgg ttacgtaaaa aacgggacac
      301 acaagcagcc tatgcttttc ttaagcggtt agtgaagcag tttgatgaac cgaaggttgt
      361 agtcacagat aaagccccct ctattacaag tgcctttaag aaactaaaag aatacggctt
      421 ttatcaaggg acagaacatc gtaccattaa atacctgaat aatttgattg aacaagacca
      481 tcgtccagta aagagacgca ataaattcta tcgaagttta cgcactgcct ctacc
//
LOCUS       DAZBTF010000210          534 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000210 DAZBTF010000000
VERSION     DAZBTF010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.219"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>534
                     /locus_tag="ACHGNS_003026"
     CDS             <1..>534
                     /locus_tag="ACHGNS_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFN6938871.1"
                     /translation="DGFKACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDL
                     KPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNT
                     SAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIR
                     GLANGRMQTYEQNTELG"
BASE COUNT          163 a           80 c          137 g          154 t
ORIGIN      
        1 tgatggattt aaggcctgta ctgatccaag acaagcagca tatgcttttg aacgaaacta
       61 tgaacgtccg gcagcgacac atcctgaacg tcaagattat gcagttaact ggtataacaa
      121 atttaaagat ttaaaaccag gaggagctac tggagaagca ggactaaaac atttagagtc
      181 tttgattgga caacgaattg gcaatggtca gtgttatggc ttgtctgcag aatattcagg
      241 atatctgggt ggttgtggca tgggtgctgg aacaaaatat ggtttaactc atgtgatagg
      301 aaatacttct gcagcatctg atattggtat tgcctatgat tggtctgctg ttggttggaa
      361 agtgattcaa aaccctagat atgatcaatt agtagttggt gcaattatta attgggcaag
      421 aggtggacaa gtgggttcat ggtttgcaga tggaacttat ggacatactg gcgttatccg
      481 aggcttagct aatggtcgta tgcaaactta tgaacaaaac acagagttag gtat
//
LOCUS       DAZBTF010000211          527 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000211 DAZBTF010000000
VERSION     DAZBTF010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.220"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            105..461
                     /locus_tag="ACHGNS_003027"
     CDS             105..461
                     /locus_tag="ACHGNS_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFN6938872.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT          206 a           80 c          108 g          133 t
ORIGIN      
        1 caataagaac aaaatgagga aaagtttaaa attcagctgt tgccaacaac tttcaataga
       61 cgaaatcttc tatttcgtta ataataagga aagaggtgaa gtacatgaaa aaattaattg
      121 gcgtacttgt cgttttaggc atatttctcg gcggaagttt tgcagcttat cattattttt
      181 acggcggcga agcttattat acaaaaatca cgacgaatgg cgaaatgtca gctacccaag
      241 cagataacgg agagaaattc acgacttata cctacaagca agcggcttat aataaagatg
      301 gtgaaaaaaa acaggtgacg cttcgcgaag aaagagaaca tccattaaag atgaatgcgt
      361 atcttaaact caaagtcaat cctcgtaaag gtgtattaag ctgggaagaa ataaaggctt
      421 ctgaagtgcc taaaaatgcg gcagaaaaaa tcgataatta gtaaatggtc ttaaaatttt
      481 attagataaa aagaggctgg gacagaagcg tttaactcca agaaata
//
LOCUS       DAZBTF010000212          513 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000212 DAZBTF010000000
VERSION     DAZBTF010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.221"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>513
                     /locus_tag="ACHGNS_003028"
     CDS             <1..>513
                     /locus_tag="ACHGNS_003028"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFN6938873.1"
                     /translation="KVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFLEILKMNS
                     DSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMPSLYEGLP
                     MVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYANKITQEL
                     KSYDVSYTTE"
BASE COUNT          206 a           58 c           98 g          151 t
ORIGIN      
        1 taaggttaat aagagaaaag aattggatat ctcaatgagt acttttgttg tgggaaacat
       61 tggacgatta gagaaacaaa aaaatcaatc ttatttgtta gagatttttt tagaaatact
      121 aaaaatgaat tctgattctt acttgatttt agtaggtgaa gggtcactta gaaataaact
      181 gaaagccaaa gcagtagaat tggatataga gaaaagagta aaatttttag gagaaagaaa
      241 cgatattccc gagttattat ctgtgatgga tgtttttgta atgccttctc tttatgaagg
      301 tctgccaatg gttggattag aagcacaagc agcaaaatta aaattagtct tagcagatac
      361 tatttccaaa gatacaaagt taacaaataa tgtggatttt gtatcattag atgaaacacc
      421 acaagaatgg gcaaaaaaag taataaaaga gccgtatgct aataaaataa cacaagaact
      481 aaagagttat gatgtttcgt acactactga aca
//
LOCUS       DAZBTF010000213          502 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000213 DAZBTF010000000
VERSION     DAZBTF010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.222"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          181 a           81 c           81 g          159 t
ORIGIN      
        1 ttttaaatag aaagacattt gggttaaatt gtgagataat aataaagaag agtttaaagc
       61 gcaccccaaa ccacttcccc ataagtgtgt tacgctttaa actcttttat atttgaagcc
      121 attaaaaagc ataccatatt tttgaaaaaa agtgagaaaa aaggcttaca attggagtgg
      181 tagttaatta gtgacttatt tttgatttta tagcactgat actataaaat atagatatca
      241 tcatattaca caatcttaat actaacttaa aaaatatctc ctttcacaag tatggtgata
      301 aaattcgttc cgggctacct ttttaggtag cctactttaa tctttatacc tttctggatc
      361 aacgaaagta tactttatat agtcataacg ccgatgatcg ctacgtgcgt ccggcacgtc
      421 agtcatgaat cggctacact agactagaca gaaaaaataa ggtgtgtaga ataataaaaa
      481 acacactgga ggatttttca tg
//
LOCUS       DAZBTF010000214          487 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000214 DAZBTF010000000
VERSION     DAZBTF010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.223"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>487)
                     /locus_tag="ACHGNS_003029"
     CDS             complement(<1..>487)
                     /locus_tag="ACHGNS_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN6938874.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IK"
BASE COUNT          142 a           92 c           86 g          167 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggat
//
LOCUS       DAZBTF010000215          468 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000215 DAZBTF010000000
VERSION     DAZBTF010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.224"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>468
                     /locus_tag="ACHGNS_003030"
     CDS             <1..>468
                     /locus_tag="ACHGNS_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFN6938875.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          155 a           86 c           83 g          144 t
ORIGIN      
        1 cttgtgttat atggggcata attaatggct atactagcca aagagccacg tatagagcgg
       61 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
      121 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      181 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      241 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      301 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      361 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      421 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaa
//
LOCUS       DAZBTF010000216          457 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000216 DAZBTF010000000
VERSION     DAZBTF010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.225"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACHGNS_003031"
     CDS             complement(<1..132)
                     /locus_tag="ACHGNS_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFN6938876.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          160 a           89 c           87 g          121 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaat
//
LOCUS       DAZBTF010000217          451 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000217 DAZBTF010000000
VERSION     DAZBTF010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.226"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>451
                     /locus_tag="ACHGNS_003032"
     CDS             <1..>451
                     /locus_tag="ACHGNS_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN6938877.1"
                     /translation="FRKGITMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKVR
                     TLNDKYVAIFMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNE
                     LLQDIYSRGVHDVLLFITDGLKGMKDTIHQIYPKAKYQHCCVHVSRN"
BASE COUNT          147 a           89 c           87 g          128 t
ORIGIN      
        1 tcttccgaaa aggtattacc atgtcggaaa tcgcagactt gattgagaaa atgtatgggc
       61 atcactacac gccccaaacc atgtccaaca tgactaaagt gctgactgaa gaagtaaatg
      121 catttaaagt tagaactcta aatgataagt atgtcgctat tttcatggat gctacttata
      181 ttccattaaa acggcaaacc gtctccaaag aagcaatcta tattgccatt ggtatacgag
      241 aagacggcac taaagaagta ctaagttatg cgattgctcc gactgaatcg acctacgttt
      301 ggaatgagtt gttacaggac atttactcca gaggggttca tgatgtctta ctgtttatta
      361 ccgatggctt aaaaggcatg aaagatacga ttcaccagat ttatcctaaa gctaaatacc
      421 aacattgctg tgttcacgtt tcccgtaata t
//
LOCUS       DAZBTF010000218          447 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000218 DAZBTF010000000
VERSION     DAZBTF010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.228"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>447
                     /locus_tag="ACHGNS_003033"
     CDS             <1..>447
                     /locus_tag="ACHGNS_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFN6938878.1"
                     /translation="SGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPITAD
                     DTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEK
                     VDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          181 a           67 c          104 g           95 t
ORIGIN      
        1 aagtggaaca acagtctttg cagtgaaaga cggcgtaagc ttaccagaag ataaacttcc
       61 agtgttgaaa gcaaaagatg gttatacaga tgcgaaatgg ccagaggaag caactcaacc
      121 aattacagca gatgatacag aatttgtatc aagtgcaaca aaactggatg ataaatctga
      181 cgctgacaaa tataatcctg aaggtcaaaa agtgactaca gaattgaata aggaaccaga
      241 cgcatttgag ggaattaaga acaaagaaga tttaccaaaa gatactaagt atacttggaa
      301 agaaaaagta gatgttagcg cagctggaaa taaaaaaggg actgttgtag tgacatatcc
      361 agatggatca tctgatgaag ttgaagtaga tgttacagtg acagacaatc gttctgacgc
      421 cgataaatat gagccaacag tagaagg
//
LOCUS       DAZBTF010000219          437 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000219 DAZBTF010000000
VERSION     DAZBTF010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.229"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>437
                     /locus_tag="ACHGNS_003034"
     CDS             <1..>437
                     /locus_tag="ACHGNS_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFN6938879.1"
                     /translation="PIENESDLEEIVQEEFIRNRKAYGTRKLKKCLAKRGLQLSRRRI
                     GRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIVTDLTYVRVGK
                     KWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDV"
BASE COUNT          136 a           82 c          105 g          114 t
ORIGIN      
        1 ccgatcgaaa atgagtccga cttagaagag atcgttcagg aagagtttat tcgaaaccga
       61 aaggcttacg gtacccgaaa attgaagaag tgtttagcaa agcgtgggct tcaactcagt
      121 cggcgccgaa tcggtcgaat catgaaacgc cgcggattga catctaccta tacgatcgct
      181 cattttaaag ggcaacgaac agcttgtaat gaagcgaaaa cagcgaatgt attagatcgg
      241 acctttacac aagaacagcc attggaagcc atcgttacgg atcttactta tgttcgcgtg
      301 gggaaaaagt ggcattatat ctgcttaata cttgatttgt ttaatcgaga aattattggt
      361 tattcctgtg gtgagaagaa agatgcctca ttggtaaaag aagcctttgg acggataccg
      421 tattctttaa cagacgt
//
LOCUS       DAZBTF010000220          425 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000220 DAZBTF010000000
VERSION     DAZBTF010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.230"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..>425
                     /locus_tag="ACHGNS_003035"
     CDS             6..>425
                     /locus_tag="ACHGNS_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFN6938880.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHW"
BASE COUNT          149 a           56 c           76 g          144 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtca ctattactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctggactaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attgg
//
LOCUS       DAZBTF010000221          414 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000221 DAZBTF010000000
VERSION     DAZBTF010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.231"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(109..>414)
                     /locus_tag="ACHGNS_003036"
     CDS             complement(109..>414)
                     /locus_tag="ACHGNS_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFN6938881.1"
                     /translation="TIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQF
                     ETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          116 a           96 c           82 g          120 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggt
//
LOCUS       DAZBTF010000222          355 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000222 DAZBTF010000000
VERSION     DAZBTF010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.235"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            56..>355
                     /locus_tag="ACHGNS_003037"
     CDS             56..>355
                     /locus_tag="ACHGNS_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFN6938882.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKY"
BASE COUNT          128 a           38 c           65 g          124 t
ORIGIN      
        1 gtaatgctta aagctatgtt atactctatt aggaaatata agaaggagta taaaaatgaa
       61 caaacataat agaaaaataa cgaataacaa agaactgatc ttgttactaa tagggatttg
      121 gggtatcttt ttactcttta atatgtatat gccgactatg agagcggatg acgtagttta
      181 tgcaagtaga ttagatgaac ttggctattt gggtgcttcg atagaacatt ataagacatg
      241 gagttcaaga attattatag aactattctt aatgttcttt tctaaacact ttatgctatg
      301 gaaattatta aattctacga ttatgttagg aattgtagta ttactttgca agtat
//
LOCUS       DAZBTF010000223          345 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000223 DAZBTF010000000
VERSION     DAZBTF010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.236"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>345
                     /locus_tag="ACHGNS_003038"
     CDS             <1..>345
                     /locus_tag="ACHGNS_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFN6938883.1"
                     /translation="AEEFIRSRKAYGSRKIKKALSKRGIQISRRKISRIMKNRGLKSS
                     YTVAYFKVHHSTCNEAKTTNVLNRKFLRDNPLEAIVTDLTYVRVGKKWNYVCFILDLF
                     NREILGYSCGEH"
BASE COUNT          129 a           60 c           66 g           90 t
ORIGIN      
        1 cgctgaagaa tttatccgca gccgaaaggc ctacggctca agaaaaataa aaaaagcctt
       61 atcaaaacga ggcattcaga tcagccgacg aaaaattagt agaatcatga aaaatagagg
      121 attaaaatcg agctatactg ttgcttattt taaagtacat cattctactt gcaatgaagc
      181 caaaacgaca aacgtattga atcgtaaatt cttaagagac aacccattag aagcgatcgt
      241 aacagacttg acttatgtac gagtcgggaa aaaatggaat tatgtctgtt tcattttgga
      301 tctgttcaat cgagaaattc tcggctattc ttgtggagaa cataa
//
LOCUS       DAZBTF010000224          330 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000224 DAZBTF010000000
VERSION     DAZBTF010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.237"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            102..>330
                     /locus_tag="ACHGNS_003039"
     CDS             102..>330
                     /locus_tag="ACHGNS_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFN6938884.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVS"
BASE COUNT          108 a           61 c           59 g          102 t
ORIGIN      
        1 ggtgaacttc accaacatct tttttgtata aaaaaatata gaaaagacac cctgaaatcc
       61 tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat actcagctta
      121 tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat tgtttaacta
      181 aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg tacaagccag
      241 aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg gggacgacaa
      301 ccgttcggtt gttactgaat gatgtgtctg
//
LOCUS       DAZBTF010000225          322 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000225 DAZBTF010000000
VERSION     DAZBTF010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.239"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(36..272)
                     /locus_tag="ACHGNS_003040"
                     /pseudo
     CDS             complement(36..272)
                     /locus_tag="ACHGNS_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          100 a           49 c           55 g          118 t
ORIGIN      
        1 atagaaggac tgaccttctg tctcagattt ttttgttatt tcacttgaat caattgattg
       61 atcaaaaaaa tcctgatttt catgttctca aatgatttaa atccataaga taatcgtttc
      121 agtgtcttga tatggggaat cttggcctct attttgccat tagaatacgg gtaaatcata
      181 gcattgtaaa taccttcttc atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca
      241 tcaagtgttt caggaagatt ttttaataac gccgtttgta taattaagcg agacaaataa
      301 aaaagccatt tatgttacac tc
//
LOCUS       DAZBTF010000226          309 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000226 DAZBTF010000000
VERSION     DAZBTF010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.240"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..309
                     /locus_tag="ACHGNS_003041"
                     /pseudo
     CDS             1..309
                     /locus_tag="ACHGNS_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          122 a           61 c           59 g           67 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcagc
      301 gatgtgcaa
//
LOCUS       DAZBTF010000227          305 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000227 DAZBTF010000000
VERSION     DAZBTF010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.241"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACHGNS_003042"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACHGNS_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFN6938885.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT           95 a           45 c           50 g          115 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgtt
//
LOCUS       DAZBTF010000228          297 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000228 DAZBTF010000000
VERSION     DAZBTF010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.242"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>297)
                     /locus_tag="ACHGNS_003043"
     CDS             complement(<1..>297)
                     /locus_tag="ACHGNS_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938886.1"
                     /translation="YVFEKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAF
                     SLLTFYPFFQLLRGEEINRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIV"
BASE COUNT          113 a           50 c           37 g           97 t
ORIGIN      
        1 gacaatcaaa tataaactaa ctatacttgt tagaacaaaa aaacaggcgt tgacttgttc
       61 ttgattagtt aaaaatatca ataaaggaat acttacccaa tatatttttc tatttatttc
      121 ttctcctctt aagagttgaa aaaacggata aaaagttagt aaactaaatg ctactggcca
      181 ctgatagttc aaggtggttg ctctccaacc ggtttccccc attactgtca atggtattaa
      241 acagtatata gagcaaacca ataataaatt tttaacattt attttctcaa aaacata
//
LOCUS       DAZBTF010000229          282 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000229 DAZBTF010000000
VERSION     DAZBTF010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.243"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>282
                     /locus_tag="ACHGNS_003044"
     CDS             <1..>282
                     /locus_tag="ACHGNS_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFN6938887.1"
                     /translation="QSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGK
                     KGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNC"
BASE COUNT           84 a           52 c           56 g           90 t
ORIGIN      
        1 aacaatcgaa gagacttttt cctgaaatca attgtgttgt cgctgatgct gggtataaga
       61 cacctaagtt tgttcacttt ttgactcatt tgaaccttcg accttgtttg ccttattcaa
      121 ggcccaaagg gaaaaagggg ctattatcaa aaaatgagtt tctctatgac gagtattttg
      181 attgttatat ttgtccgcaa gatcaaatgc tcgctttttc aacggttact cgagaagggt
      241 accgagaata caaatccaat ccaaaagaat gtgtgaactg tc
//
LOCUS       DAZBTF010000230          253 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000230 DAZBTF010000000
VERSION     DAZBTF010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.245"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>253
                     /locus_tag="ACHGNS_003045"
     CDS             <1..>253
                     /locus_tag="ACHGNS_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFN6938888.1"
                     /translation="GFTKQSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSY
                     PLVGPATWDGKVYVQNLFNAAGIKEPITSLDAQVRLLIWT"
BASE COUNT           80 a           40 c           58 g           75 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga cat
//
LOCUS       DAZBTF010000231          248 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000231 DAZBTF010000000
VERSION     DAZBTF010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.248"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>248)
                     /locus_tag="ACHGNS_003046"
     CDS             complement(<1..>248)
                     /locus_tag="ACHGNS_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFN6938889.1"
                     /translation="KQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFD
                     NAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWW"
BASE COUNT           82 a           46 c           37 g           83 t
ORIGIN      
        1 ttccaccaat tgacatagtc aaataactcc aaatccaatt gttgtaaggt ttcaaatgtg
       61 tattgataga caaattctac tttcaacgac ttataagttg attcagctac ggcattatca
      121 aaaggacagc ctttatgact caatgatcga ttgatgtcaa aagttgttaa taattcatca
      181 atagcttggt tatcaaactc ttttccacga tcagtatgaa aaatctcaac ctctgtcaga
      241 ggttgttt
//
LOCUS       DAZBTF010000232          237 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000232 DAZBTF010000000
VERSION     DAZBTF010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.250"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..237
                     /locus_tag="ACHGNS_003047"
                     /pseudo
     CDS             1..237
                     /locus_tag="ACHGNS_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT           95 a           39 c           44 g           59 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatc
//
LOCUS       DAZBTF010000233          233 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000233 DAZBTF010000000
VERSION     DAZBTF010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.251"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..>233
                     /locus_tag="ACHGNS_003048"
     CDS             22..>233
                     /locus_tag="ACHGNS_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFN6938890.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEI"
BASE COUNT           87 a           44 c           44 g           58 t
ORIGIN      
        1 atacaaggag agtgaacata aatgacttat acccatctta caccaaacga gcttgtaatg
       61 atagaagcat attttcatca agaaactccg gttgctatcg ttgcgaagca gcttaaacgt
      121 ggacgccaaa caatttacaa tgtctataac tttctcaaat gtggtggaac agcacttgaa
      181 tactttgaac aatacaaaga aaataagcga cgttgtggga gaaccgaaat cat
//
LOCUS       DAZBTF010000234          233 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000234 DAZBTF010000000
VERSION     DAZBTF010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.252"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           79 a           35 c           45 g           74 t
ORIGIN      
        1 tgagaaaaag catggaatgc gctgcatcag gtatcgaggc ttagaaaaaa atcaggtgca
       61 tacgatgctt gtttttgccg aaatgagtct gaaaaaattg gtcacttagc agtgggagca
      121 tatgtcttca tattttttat tatacaaaaa tagacaaaag tatcagtcta cactcttagt
      181 aaagaggaaa ctgatacttt tgtctacagt atgatatttc ttacatctac ttt
//
LOCUS       DAZBTF010000235          227 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000235 DAZBTF010000000
VERSION     DAZBTF010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.254"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>227)
                     /locus_tag="ACHGNS_003049"
     rRNA            complement(<1..>227)
                     /locus_tag="ACHGNS_003049"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           48 a           77 c           45 g           57 t
ORIGIN      
        1 atcgtctgca ttccttagcc tcctgcgtcc ctccattgct caaacaaaaa caacgggtac
       61 aggaatatca acctgttatc catcgcctac gcctgtcggc ctcggcttag gtcccgacta
      121 accctgggcg gacgagcctt ccccaggaaa ccttagtcat tcggtggaca ggattctcac
      181 ctgtctttcg ctactcatac cggcattctc acttctaagc gctccag
//
LOCUS       DAZBTF010000236          224 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000236 DAZBTF010000000
VERSION     DAZBTF010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.255"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>224
                     /locus_tag="ACHGNS_003050"
     CDS             <1..>224
                     /locus_tag="ACHGNS_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFN6938891.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAA
                     ESQTPAKPAAEPTVDTESAGSSSEGVFQFK"
BASE COUNT           70 a           47 c           58 g           49 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agtt
//
LOCUS       DAZBTF010000237          220 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000237 DAZBTF010000000
VERSION     DAZBTF010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.256"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..175)
                     /locus_tag="ACHGNS_003051"
     rRNA            complement(<1..175)
                     /locus_tag="ACHGNS_003051"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           48 a           60 c           49 g           63 t
ORIGIN      
        1 cctgtttcca agtgttatcc ccttctgatg ggcaggttac ccacgtgtta ctcacccgtt
       61 cgccactcct ctttttccgg tggagcaagc tccggtggaa aaagaagcgt tcgacttgca
      121 tgtattaggc acgccgccag cgttcgtcct gagccaggat caaactctca taaaaagttc
      181 gaacaatctt gcgattgtta agctcaattt gtttgctagc
//
LOCUS       DAZBTF010000238          210 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000238 DAZBTF010000000
VERSION     DAZBTF010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.denovo.257"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           76 a           35 c           37 g           62 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat
//
LOCUS       DAZBTF010000239        11111 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.guided.085, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000239 DAZBTF010000000
VERSION     DAZBTF010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.guided.085"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..1524
                     /gene="eat(A)"
                     /locus_tag="ACHGNS_003052"
     CDS             22..1524
                     /gene="eat(A)"
                     /locus_tag="ACHGNS_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFN6938892.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            1591..2118
                     /locus_tag="ACHGNS_003053"
     CDS             1591..2118
                     /locus_tag="ACHGNS_003053"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFN6938893.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            2329..3390
                     /locus_tag="ACHGNS_003054"
     CDS             2329..3390
                     /locus_tag="ACHGNS_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFN6938894.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            3393..4289
                     /gene="murQ"
                     /locus_tag="ACHGNS_003055"
     CDS             3393..4289
                     /gene="murQ"
                     /locus_tag="ACHGNS_003055"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFN6938895.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            4303..6285
                     /locus_tag="ACHGNS_003056"
     CDS             4303..6285
                     /locus_tag="ACHGNS_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFN6938896.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6350..6796)
                     /gene="nrdI"
                     /locus_tag="ACHGNS_003057"
     CDS             complement(6350..6796)
                     /gene="nrdI"
                     /locus_tag="ACHGNS_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFN6938897.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            7007..8431
                     /locus_tag="ACHGNS_003058"
     CDS             7007..8431
                     /locus_tag="ACHGNS_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFN6938898.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(8612..9385)
                     /locus_tag="ACHGNS_003059"
     CDS             complement(8612..9385)
                     /locus_tag="ACHGNS_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFN6938899.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(9382..10287)
                     /locus_tag="ACHGNS_003060"
     CDS             complement(9382..10287)
                     /locus_tag="ACHGNS_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6938900.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(10441..10761)
                     /locus_tag="ACHGNS_003061"
     CDS             complement(10441..10761)
                     /locus_tag="ACHGNS_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFN6938901.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         3578 a         1978 c         2365 g         3190 t
ORIGIN      
        1 ttttttacga ggtgatggaa aatgtctaaa atcgaaataa aaaatctgac attcggctac
       61 gacagccaag gcacattatt atttgaacaa gcaaatctaa attttgacac acaatggaaa
      121 ctaggactta tcggacgaaa cggtcgagga aagacaactt tactgaatat tctacaaaac
      181 aaactacctt atcaagggca agtaatccat cagcaagaat ttgcctattt cccgcaacag
      241 acaaaagata aagaacgttt aacctattac gtgttaaatg atattacgga ttttgagata
      301 tgggaaatcg aaagagagct ccaattgatg caaacagatc ctgaaatctt atggagagaa
      361 ttcagcacac tatcgggggg agagaagaca aaagtcctac tggcactttt atttgtggat
      421 gacactcatt tcccgttaat cgatgaacca acgaatcatt tggatatctc tggtagaaaa
      481 caagtagcgg cttatttgaa aaagaaaaaa caaggcttca tcgtggtcag ccatgaccgg
      541 ggatttatcg atgaagtagt ggaccatgtt ttagcaatcg aaaaaagtca actggaactt
      601 tatcaaggga atttctctat ctatgaagaa cagaaaaaac ttcgtgatga atttgaaatg
      661 gctcaaaatg aaaaattgaa aaaagaagtc agtaggctaa agaaaacagc agctgaaaaa
      721 gccgaatggt ctcgttcccg agaaggagat aaaacaaaga aacaagtcgg attcatcgat
      781 actgaatcta gacgagtgaa taaaggagca gtgggtgctg atgctgcacg gacgatgaaa
      841 cgatccaaag caatcgtgaa tcggatggag acccagatca gcgagaaaga aaaactatta
      901 aaagatatcg aatatatcga ttctttgacg atgaatagcc aagcgtctca ccataagcga
      961 cttttaagcg tagaagatct tcaattaggg tatgaaaatc tgttattcga gccaattcat
     1021 tttacaatcg agcctcatca gcgggtggcg atttcaggtc ctaacggtgc aggaaagtca
     1081 tccattatcc attatcttct gggggcattc aacggcaagg ttataggaga aaaataccag
     1141 ccaaaacatc tgagcattag ttatgcaagc caaaattatg aagacaatcg aggaacgttg
     1201 gcggaatttg cagagaaaaa ccaagtagac taccaagcat ttttgaacaa cctccgaaag
     1261 cttgggatgg aaagagatgt ttttcataac aagatcgagc agatgagtat gggccaacgg
     1321 aaaaaagtgg aattggctaa atctttatca cagccagctg aactatatac atgggatgaa
     1381 ccattgaatt atttggatgt cttcaatcaa gaacaattag aacaactgat cttgaacgtg
     1441 aaacctgcca tgttactagt ggaacatgat caaaccttcc tggataaagt atctactgag
     1501 attatttctc ttgagagaat ctaacccttg taatctcaag aacaacaagg tatggttaag
     1561 gggaactttg ccaacgaagg agagaatcac atgctttatg taacgagaca cggagaaaca
     1621 acttggaacg cacaaggatt agtttgcgga cgggcagatg tgccgctgac tgaaaaagga
     1681 cagatgcagg cacagaaact agcagaaaag gtagtagatt tgcctgttcc aatcacgaaa
     1741 atcatccatt cccctcttca acgagcaaga gatacagcac aagcagttgc tgatcgtctc
     1801 agcttgccgc tgaccgttga tgaacgattg gtagaaatgg attttgggga ttatgatgga
     1861 ctgccaagca aagatgagaa ctttcaaaaa gcacgtttag cgttcgctgt acgattccca
     1921 aatggggagt cagtcttaga tgtgtatgca cggatcgttc ctttattgaa agaatgcatc
     1981 gaagacgaag aaaatgtcta tctgttagtt tgccataatg ccttgataag agtgatcaat
     2041 gcttattttc atccgatgcc taatgagggg ttctttacat tcatggtaga taatacagaa
     2101 ttggtttctt atgagtaatt atgtttttgt gttatttaaa taaaatattt caaggcactg
     2161 cttttgctta gcagtgcctt ttttaggtta tgacaattct tttgataata taaactcaac
     2221 ctttagaaat gaaatttaat tattttaata aaagtattga aattttgttt ctttagatat
     2281 atactctagt catagaaatg aagacggata cagaaaaaga gggataatat gtttggcttt
     2341 tcaatattta tgaacgattc tttgtcagaa gagaagaaac agtatatccg ccaaatggca
     2401 tccaatggct ttgtcggtat tttcacttcc atgcatatac cagaagatga tgtttcggca
     2461 tatcaaaagc gattgatcga actaggaagc gaagcgaaaa agaataaact agaattgatg
     2521 gtcgatattt caggagaggc attggaacga gcgggttttt cattagaaga tctaacgcct
     2581 ttgaaagcaa ttggtgtaac aggtttaaga atggattatc atatttccaa tcagcagatt
     2641 gccgactggt cacatcaatt gaaaatcagt ttgaatgcta gcacgatcac gcctaaagat
     2701 atcgatgaat tgaaagaagc agaagctgat ttctcacaga ttgaagcttg gcataattat
     2761 tatccacgtc ccgaaacagg gctggacaaa gaatggtatc agaaaaaaaa tcaatggctc
     2821 aaaattcaag gtctccttgt ccaaggtttt gtaccaggag atacagagtt gagaggccca
     2881 ctatatcaag gattgccaac tttggaagaa catcgaggga tccatcctct tgcaggcgct
     2941 ctagatctat tagcaagtaa cacggatatt gtttacatcg gagatgcagg tctgtcagaa
     3001 aacgttcagg agcaatttgc ttctttccaa aaagaacaga cagtcttgct ccatacggaa
     3061 cctgtagatg aagaatttta cgaatatata ttagggaagc atacgaatcg gcaagacgat
     3121 gcaagagatg tcatacgaag cgtagatgca cgttttcgag agattccgcc tatcccagca
     3181 agaaatacag ctacgcgtat gaaagggtct attactttag ataacgaaaa gtatttgcgc
     3241 tatatgggtg agatccaatt aaccaaatat gatttgccag cggatgaaaa agtgaatgta
     3301 gtagctaaag ttatcaaaga agagctacca ttgatcaacc aaatcaaagc tggtatgaac
     3361 tatcaattta taagaaaaga aggaagataa aaatgagcaa aatcaattta gagaacctaa
     3421 caacggaacg cagaaaccaa gaaacttttg gattagatga gatgagtatt tctcaagccg
     3481 ttcaactaat gaacaaagaa gatcagaaag tggccgctgc ggttgaaaaa gaactgacaa
     3541 gtatcgaacc agtgattgct aacacgattg agtcctttaa aaaaggtgga cgtctgattt
     3601 atatgggagc cggaacaagt ggaagactag gtgtgttaga tgctgctgaa tgcgtaccga
     3661 cattcggtgt agaaccggaa atggtcatag gtctaattgc tggtggagaa gaagcaatga
     3721 ctgtagctgt cgaaggggcc gaagattctt atgagctagg gcaagaagat ctaatcaagc
     3781 tgaatttgac aaaaaatgat atagttattg gtatcgcagc tagcggtcgt acaccatatg
     3841 tgatcggtgg attagattac gcaaaatcga tcggtgctgt cacaggaacg atttcttgca
     3901 ataaacaagc cgaaatcagt aaacacgcgg atttcccaat cgaagtcgat gcaggtccag
     3961 aatttttgac aggctctact cgattgaagt caggaacagc acaaaaactt atcttgaata
     4021 tgatttctac tatcagtatg attggtatag gtaaagtatt caataacttg atggtcgatg
     4081 tcaaaccaac aaatgaaaaa ctaatcgaac gctcaaaacg aatcatcatg caagcgacag
     4141 aagctgatta cgaaacagct gaaaagtact ttgaagaagc agatcaaaat gttaagctgg
     4201 ctatcgtcat gattttgaca aatagtgata aagaggaagc tcaagcaaaa ttagctgaag
     4261 caaacggatt tgtaaaaggg accttatgag ggggaaaaga ccatggcaaa aaacgaactg
     4321 actttggaag aactggcaaa acaaatttat aaaggggcag gcggcatggg caatgtagaa
     4381 agcattgtcc actgcatgac tcgtgtgcgt atgaagatca tcaatgatga tcttgtaaat
     4441 gtctctgaat taaaagcgat tccaggagtg cttggtgtag tagaagatga acagcttcaa
     4501 gtcatcatcg gacctgggaa agtgaacaaa gtagcacagc aaatggttga tttggcaggc
     4561 gtgaaattag gagaaaaact ttcttctgta agtacagcta cagcaaattc atcagcatct
     4621 ggaaaagacc gtgtaaacga acgggcacaa gaaatgaaag cggcacaaaa atcgaaacaa
     4681 aaaccatcta aaatcaaatc ggtattaaaa gacatttcta atatctttgt gccaatgatt
     4741 cctgcatttg tcggtactgg gatcgtggca gggattgccg cagttttagc aaacttagtc
     4801 acagccggaa cacttgatgc agcaacatgg caacaatata tcgatgtgat gaacatcttg
     4861 aaaaacggga tgtttagcta cctagttatc tatacaggta tcaatgctgc acaagttttt
     4921 ggtgcaacac caactcttgg tggagttatc ggggccgttg tgatgctgac agggatgaat
     4981 ccggaagcac cactgaaaaa cttatttact ggtgaagctc ttgcagctgg acaaggtggg
     5041 atcctaggtg tcatcttcgc tgtttggcta ttgtcaatcg ttgagaaaaa actgcataaa
     5101 gtagtacctg atgcggtaga tatcatcgtt acaccaactc tttcattgat tgccatcggt
     5161 ctgattgaaa ttttcttcat catgccttta gcaggtttca tttctgatgg catggtcggt
     5221 gggatcaact gggtacttgg cgttggtgga gcatttgccg gcttcgttct aggcacatta
     5281 ttcttgccga tggttatgtt cggactgcat caaattttga ctccaatcca tgtgcaaatg
     5341 attgatgaaa ctggtcgtac gctattgctt ccaattcttg cgatggctgg tgcaggacaa
     5401 gtgggtgcag cattggcatt atggattcgc tgcaaaaaag ataaagaatt aacagaaatg
     5461 atcaaaggag ctttaccagt cgggatccta ggaatcggcg aaccattgat ttacggagta
     5521 acattacctc taggacgccc atttattact gcttgtatcg gtggaggtat cggcggggca
     5581 gtgatcggaa tgatcggaaa tgtcggggct atcgctattg gaccttcagg agccgctttg
     5641 atcccattga tctctgacgg taaatggtta ggctacatcc ttggattatt ggcagcttat
     5701 gctggtggct tcgttgctac attcttcttc ggtattccta aagaccgttt agaaaaagaa
     5761 gaattagcga ctgaaacagc cacagtaaat gaagtgcctg aaaaagtagt acctcaaact
     5821 tcagctgaaa gtgtgactat cctctcatca gttgctgacg gaacagttgt ttctttagat
     5881 aaagcaagtg atccagtttt tgcacaaaag atgatgggtg aaggctactt tgtagaacca
     5941 gaaaacggac atatctattc tccagttact ggggtagtca gctctgtctt cccaacgaaa
     6001 cacgctattg ggatcacaac gccaaatggt ttagaaatct tgcttcatat gggaatcaac
     6061 acagttgact taggcggtac gccatttgat gtgaaagtaa cagaagggca gcaagtgaca
     6121 cctgatacgc ttgtagcaga agctgactta gaagcgatca aagcagcagg caaagaaaca
     6181 tccatgatcg tcttggtaac caatatggat cgtgtaaaaa actttgtctt ggaaaaaaca
     6241 ggtaaagcaa aagcaaaagc acctgtgatg gatgtcaaag cttaatatga tagaaacaat
     6301 agaaacgaaa gaagaaactg aaatctattt ttcagcttct tcttttttat tatttattgt
     6361 tttctgctaa aatctgataa attcgtgtca catcagatgg tgttccccgt aattcataat
     6421 ctgccaagaa aggaaagcca aatgtttgtg catattgttt ggctgttaga caatactgat
     6481 tattgaagtt tttatttccg ctaccgacta caccaagaca aagtttatga ttgtcttcat
     6541 gttccagata ttcacgcatc gtttcggtca agatttctgt atcaccgtta tctaaaccat
     6601 ttccgccgtc taaataagta ggaacgaaag taaaaaatgg ttctgtttct ttcgtaaaat
     6661 cactattttc atggatttct tttaaagtaa cttcaggatt gacaggatct tctgcatgca
     6721 ttttttcagc atattctgct agatgtttgg caaaggcacg cgtatttcct gaaatcgata
     6781 tatataaaat gttcatggat catcctccaa ttctttcctt ttagtatagc atctttttta
     6841 agttttttgg tggtgtaatt tggaaaggga ctaggcgctt tgcttgtata atgagaaaaa
     6901 agccggtaga attatatttc tattaaagtt tttattaatt aagtaaaaat aaaaaaggtt
     6961 tgttatactt tttgataaaa gcggttttat tttggaggat gcttacatgg atacagagca
     7021 acagaaaaaa ggaattggtt taattccact tgctgcctta gtaattggtt cagcgattgg
     7081 aggtggcgta tttggtatta tgacggacat ctccggatcg gctggcggcc cagctatttt
     7141 cagttgggta ctcgtaggaa cagcaatgtt gatgctctcc ttgtcgatca ataatatcaa
     7201 taaaaaacgt ccagatttaa aaggcgggat ctttagttac gcagaagaag gttttggccg
     7261 tttcgctggt tttatcagcg gttggggtta ctggctcaca tgctggttag gcaacgtagc
     7321 ttttgcaaca ttgttgatga gcgcattagg atatttcttc ccagtatttg aaggtgggca
     7381 aaatctggca tcgatcgtga tgagtacagt ctttttatgg ggatatgcat ggctagtgaa
     7441 tcggggagtc gaaaatgcta gtttcgtcaa tgctatcgtc acattctgta aattagtgcc
     7501 attattttta tttattgtag tagctatcag cacgttttcg atccaacaat tcattgataa
     7561 cttttctagc gacattatat tgaatgcgaa tggacagccg atcccgtttt ccagtcaagt
     7621 attgagttgc atcatggtga tgctttgggt atttgttggt attgaaggag cctctgtcgt
     7681 aggttcacga gcaaaaaaac aatcagatgt tgggaaagca acgattctag ggatattcgg
     7741 cttgattatt atttacgtct tggtttccat gcttccatat gggacaatga cgatgaatca
     7801 gctgcaaacg atcaaggcgc agcctgctat gggctatgtg tttgaaatga tggttggtaa
     7861 atggggagca gtcgtgatca acggcggttt gatcatttct ttagttggtg tttggctctc
     7921 atggacgatc ttaccgattg aaacaatggg aaacatggct gaagacggac ttttgcctaa
     7981 agcatgggga aaactcaaca aaaatggggc accaacttat gctatcgtat tgacgacgtt
     8041 atgtacgaat gtctttttac tttcattatt attcactgac agtgcttata actttgctta
     8101 cacacttggt acagcagcta ttttcttcac gtggcttttc ttaggattgt accaaatgaa
     8161 actttcttat cagcggaaag aatgggtcca atttttagta gggcttttag caagtgcctt
     8221 tcaagtatgg gctatgctag tcgtagcctt taatgaagta atgcttgttc ttgttctttt
     8281 cttgccaggc atttattttt acctcaaagc acgtaaggag caaaacgagc ctgtacaaca
     8341 tatccactta gataagattt tacttatagg tgtgacgatg gtcggagtga ttgcaatggt
     8401 tttatttgcc actgggatgc ttcacgtata gaagtataaa agaagtataa agagaggttg
     8461 tgagcctgtc gtttattcaa aaacatacta ggaaaggagc tgtgacacaa atgttgtcac
     8521 agctcctttt tcagaataaa cggtgtttaa gagacagcca atcggcaata aggctgaacg
     8581 tctctttcac agcctctttt ttgatgagtc atcatacatt ttttcgctta aatacgagat
     8641 atcctaatgc taagaacaaa acgatgtaag ctaaattacc gaatagcatt tgattagtag
     8701 aaagatgagt caattgcttc atgatctctt cgtttccaat ttggttttgc agattcaaca
     8761 tactgatcgg attccatttg atccactccc atttttggat gagtgcaata aggataccag
     8821 atatgattga cgaagcaaaa taaaagacga ttcctgctga gatggatgcg ccggaactat
     8881 tgataaagca agcaatcatc aacactaaac ttaaaatcag ccataaaccg atataactac
     8941 ccaaagtata agtgaacaag ctttggatca gtgtttgacc agtagaaacc ttctccgtga
     9001 aggaaatcga agggaaaaga atcagtttca tcaacacagt gacaataata gctaaaaggt
     9061 ataaataaac agaataaata actaatgtga tccatttgct tactaatatt tctcctcgag
     9121 aatactttcg ataaaggaga tttttcaatg taccatattg tgcttccatc gaaatgatcg
     9181 tgcttgcagc tgcaatcatg ataaagacaa tccaagatgt ggcactaaaa agctgagcag
     9241 tcatttcaac tggatcaatg atcgtcgtgt attttttggc tagaagtgct gttcctatga
     9301 gcaaaacaac taatagaaca gataaaacag cagttgactt tttcttgatc aatttaaata
     9361 attcttgttt tacaagcaat gtcatttttc ttcttccccc tttactgaat ccagaatttc
     9421 caatagagaa gattccaaat cattcgtcag atgctggacg tctgtcacat ggatattttg
     9481 tgcagacaat agctggatca cggttcctaa taattcttcg ttttctttta aaagcttgat
     9541 tttatcagga tcttccaaag tatatccagc ttctgtcaag atcgttcgag cagctagatc
     9601 atttgttgta gagataacga tgaaatcttg cgctgtttgg ttcaattcag ccattgtaca
     9661 ttgtttgatg atctttcctt gattgatcag gatcagctca tcgaccattt tttcaagctc
     9721 gctgagtaag tgactggaga tcaagaaaga ggtgccctga gtagccaagt cggtaatgat
     9781 ttttcgcaca tctcttgttg cttgtggatc caaaccgttc atcggctcat ctaaaataac
     9841 gatagacgga tggttcagta aggcaagagc aattcccaat ttttgtttca ttcccaacga
     9901 gtaggatttc gcttttttgt gaatgtactt ttccatcttg agctgattca cgatagtgtc
     9961 gatagcagat acatcgttgg tttcagagaa taacttcaga tgatcatagc ctgttaagaa
    10021 tggatagata cccgggtatt cgatcaatgc tcctacttgt tccaaccctt tatgactaga
    10081 gggagaaatc tcattttcac caatacagat tgttccatct gagtaatgga tcagtcctaa
    10141 aatagctttc atgatcgttg tttttccagc gccattagga cctactaatc caacgatttt
    10201 gccaggggat aagctaaaac tgacatcgct gagggcttgt ttgtgaccaa agttcttgga
    10261 taattcttgg accgaaagac ttttcaaaaa tattcactcc ttttctatag atagtacaag
    10321 catacacctg atgagtggag aagtcgctag gtcattggac ttatttctta ttttttcata
    10381 aaaaaattga atattaaata aaaaggctta ttttttctga tcagtactta ttccaagtgt
    10441 ctagtggccg ctagtaatct tgatgccaat gatactgatg agtagcatcc ccacaaagaa
    10501 ccaagtggaa agattcagct tatcatgaaa caagaccaca ccaattatga ccgaaccaac
    10561 tgcgccaatc cctgtccaga taggataaga gagactcatc ggcaaacttt tgattgcctt
    10621 tgacaaaaag taaaaactag caatcatgcc aatcactgtg taaaaagaat aattgatttt
    10681 agaaaaacca tcactccatt tcatcatcgt tgaccagaat acttccaaaa ttcctgctat
    10741 gattaattcc atccatgcca tacttacatt cctccttaac tataaaaaaa tacacaaaaa
    10801 gaggaaaatc atcccttcaa agacctaatg gaatgatcat cctctttatc ctacccggtg
    10861 gcagattgta tatctttttc tgtagtatag cacacctatc cagatatcgg caatctcata
    10921 ctttttagta aagtgtacca agcactgacg gatatatcgt tagcggaaaa gaagatgaat
    10981 gctttgcaat ttcatagaaa gaagttgtta tttattaatc atttaaaaaa aacagtaact
    11041 agaccttatt tctatatatt atcgggcaat aaattaaaaa aagctttacc ttacaataag
    11101 taagataatt t
//
LOCUS       DAZBTF010000240         8537 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.guided.101, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000240 DAZBTF010000000
VERSION     DAZBTF010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.guided.101"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            335..994
                     /gene="vanR-B"
                     /locus_tag="ACHGNS_003062"
     CDS             335..994
                     /gene="vanR-B"
                     /locus_tag="ACHGNS_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFN6938902.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            997..2358
                     /gene="vanS-B"
                     /locus_tag="ACHGNS_003063"
     CDS             997..2358
                     /gene="vanS-B"
                     /locus_tag="ACHGNS_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFN6938903.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
     gene            complement(2449..3750)
                     /locus_tag="ACHGNS_003064"
     CDS             complement(2449..3750)
                     /locus_tag="ACHGNS_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFN6938904.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            4029..4835
                     /locus_tag="ACHGNS_003065"
     CDS             4029..4835
                     /locus_tag="ACHGNS_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HFN6938905.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            4853..5560
                     /locus_tag="ACHGNS_003066"
     CDS             4853..5560
                     /locus_tag="ACHGNS_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HFN6938906.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            5557..6528
                     /locus_tag="ACHGNS_003067"
     CDS             5557..6528
                     /locus_tag="ACHGNS_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFN6938907.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            6521..7549
                     /gene="vanB"
                     /locus_tag="ACHGNS_003068"
     CDS             6521..7549
                     /gene="vanB"
                     /locus_tag="ACHGNS_003068"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFN6938908.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            7555..8163
                     /locus_tag="ACHGNS_003069"
     CDS             7555..8163
                     /locus_tag="ACHGNS_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFN6938909.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(8229..8402)
                     /locus_tag="ACHGNS_003070"
     CDS             complement(8229..8402)
                     /locus_tag="ACHGNS_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938910.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         2450 a         1729 c         2130 g         2228 t
ORIGIN      
        1 ctgaggcttg gggtgtcccc aacaagcaaa acgagaacgc cgcaggcggg caagtggtgg
       61 atcagaatat ccgtaaagga tattctgatc cattgcttgc cagtattatc gggatgtttt
      121 gacaaccgct gtaatcgaaa catcccaaac attaagaagg tgttaattat tattatcttg
      181 atttaattcg tttaaaccaa taatatagat acatacacca tgaaaagaag cgagtatccc
      241 aatgacggat gattaaaatc cgaaaagaaa tcgctttgaa actacaggga aactacagac
      301 tgttatgtta tcttcttaaa atggagggat ttttatgtcg atacgaattc tacttgtcga
      361 ggatgatgat catatctgta atacagtaag ggcgtttctg gctgaggcag gatatcaggt
      421 ggatgcctgc acagatggaa atgaggcata caccaagttt tacgaaaaca cttatcaact
      481 ggttattctt gatattatgc tgcccggtat gaacgggcat gaacttttgc gtgaatttcg
      541 tgcgaaaaat gatactccca ttctgatgat gacagccctg tcggatgacg aaaaccaaat
      601 ccgggcgttt gatgcagagg cagacgacta tgtaacaaag ccattcaaga tgcagatttt
      661 actaaagcgg gtggaagccc tgctgcggcg cagcggtgcg ctggcaaagg aaatccgtgt
      721 cggcaggctg acacttctgc cggaggattt cacggtactt tgtgacggta cagagctgcc
      781 cctgacacga aaagaatttg aaatcctttt gctgctggtg cagaacaaag gcagaacctt
      841 aacgcatgaa atcattttgt cacgtatatg gggatatgac tttgagggtg atggcagcac
      901 agtccacact catatcaaaa atttgcgggc caagctgccg gaaaatatca tcaaaaccat
      961 tcgcggtgta ggataccgat tggaggaatc ataataatgg aaagaaaagg gattttcatt
     1021 aaggtttttt cctatacgat cattgtcctg ctactgcttg tcggtgtaac agcaacactg
     1081 tttgcacagc aatttgtgtc ttatttcaga gtgatggaat tacagcaaac agtaaaatcc
     1141 tatcagccat tggtggaact gattcagaat agcgataggc ttgatattca agaggtggca
     1201 gggctgtttc actacaataa ccaatccttt gagttttata ttgaagataa agagggaagc
     1261 gtactctatg ccacaccaaa tgccaataca tcaaatagtt ttagacccaa ctttctttat
     1321 gtggtacata gagatgataa tatttcgatt gttgctcaaa gcaaggcagg tgtgggattg
     1381 ctttatcaag ggctgacaat tcggggaatt gttatgattg cgataatggt tgtattcagc
     1441 cttttatgcg cgtatatctt tgcgcggcaa atgacaacgc cgatcaaagc cttagcggac
     1501 agtgcgaata aaatggcaaa cctgaaagat gtaccgccgc cgctggagcg aaaggatgag
     1561 cttggcgcac tggctcatga tatgcattcc atgtatgtca ggctgaaaga aaccatcgca
     1621 aggctggagg atgaaatcgc aagggaacat gagttggagg aaacacagcg atatttcttt
     1681 gcggcagctt ctcatgagct aaaaacgccc atcgcggcta caagcgttct gttggaggga
     1741 atgcttgaaa atatcggtga ctacaaagat cattctaagt atctgcgcga atgcatcaaa
     1801 atgatggata ggcagggcaa aatcatttcc gaaatactgg agcttgtcag cctgaatgat
     1861 gggagaatcg tacccatagc tgaaccgttg gacatagggc gcacggttgc cgagttgctg
     1921 cccgattttc aaaccttggc agaggcaaac aaccagcggt tcgtcacaga tattccagcc
     1981 gggcaaattg tcctgtccga tccgaggctg ctccaaaagg cactatccaa tgtcatattg
     2041 aatgcagttc agaacacgcc gcagggaggc gaggtacgga tatggagtga gcctggtgct
     2101 gaaaaatgcc gcctttttgt tttgaacatg ggcgttcaca ttgatgatac tgcgcttcca
     2161 aggctgttca ccccattcta tcgcattgat caggcgcgaa gcagaaaaag tgggcgaagc
     2221 agaaaaagtg ggcgaagcgg tttaggactt gccatcgtac aaaaaacgct ggatgccatg
     2281 agcctccagt atgcgctgga aaacacctcg gatggcgttt tgttctggct ggatttaccg
     2341 ctcacatcaa cattgtaaat atttaaaact taaatgattt tgggctcttt gtcaaatagg
     2401 actgatagag tgagataaga acatctaagg tagaattcct tttctacttt agatgttttt
     2461 tgattcatag gtaatcagat tatttagtaa gaaaattctg gtcttcattt tccgaaagct
     2521 tcgaaagcca taagcgactc gtttcaatgc tttgatgtgg gtatgcagat tttctatttt
     2581 ggcatttgaa taaggcaatt cgagggctaa acgaatgccg gattcatagt ttataaaagc
     2641 tttttttata gaatgcagtt cttttggaac ggaattgggc atcgttctta caagtttaaa
     2701 gaaagtgtcc gcctctttgt cgtgaaacgc ttgaagaatt tcttgatagt aaaaataggc
     2761 gattcgtaat tcgtcagaga tcgaaagcag acgatccacc acttcacttt cggtgagata
     2821 cgcccaacca aaattgcgcc atttggtcca tttgtcataa acaagcttgt cctgtcgttt
     2881 gatcagaagc cgagacaacg cctttaattg tcgataatat ttggcttgtg ttggatcgtt
     2941 tcgatcaaag cttttcatga ttcgtacgcg aatatcctca aatttacgaa tgagatgttt
     3001 gacaatgtga aaacgatcca caacgagttt tgcctttgga aaacattcct ttatcagaga
     3061 ggcataagaa acattcatgt ccgtcacgat gacctttacc ttcaatcgtg cgcgtcgttc
     3121 aaattgcata aaataagaaa cgagagcctt tttcttccgt gaaggaagaa tatccagtaa
     3181 ttttccagta tcgccatcca tacaagaaaa ggagtaggga ccgacttgat ttttcagtga
     3241 acgaaattca tccattaata aacaacgcgg taaccaattt ctttttactt ttgtcatggg
     3301 gatcagagaa gcgagtgtcc gagaaacggt cgaccaagaa acgccgcaat gcttagcaat
     3361 tgtcgtcatt gcttgttttt cagttagctc ttctaaaatt ttatagcgca cttgtttagc
     3421 gataaagcag tttctatcga caatgtaggt attcgccgta aaataggtgt ggcagccacg
     3481 acagtggtag cgttgtttcg ctaaagcaag ataagtcagt tggttcgcac atttattaag
     3541 ttgaatgagc gattttttct ttccgttctt aacgatcatg ccttcatgat tcatctgttt
     3601 acacaaagga cagcgacgaa tttctttact taacgtcgca gaaacaacaa gactcttgat
     3661 attcttaatg ctcttcgatt ccacatttgt gatgaaaatg tttttatctt tgattccaag
     3721 tagtagtttg atatgatgaa gatcaggcat acagaacgct cctttgattg tgttgtggtg
     3781 attcaattct aacggagctt ctgtatgttt tgttttttca tgctcaaaag ggaccgatag
     3841 aatgattttc tatcagtcct agaaattata gagccgattt tgaccgacag gtataaccct
     3901 gccggtcttt ttttgctgct acaggaaaac tacagactgg ctacagggaa agtacagatg
     3961 cgcttgccat aataacaatc gtaccagcca caaatcgtag ttttattgca aaggaggcat
     4021 tcgatcaaat ggaaaaaagc aactatcatt ccaatgcgga tcatcacaaa cggcatatga
     4081 aacaatccgt ggaaaagcgg gcttttctat gtgcgttcat tatttcgttc acagtctgca
     4141 cgctgttttt ggggtggaga ctggcttccg tattggaggc aacacagata ccgcccatcc
     4201 ctgcaactca tacaggcagc agcactgacg tagtggagaa tttggaggaa aacgctcttg
     4261 ccaccgccaa agaacaggga gatgaacagg aatggagcct gattttagtg aacaggcaga
     4321 accccatccc cgcacagtac gatgtggagc ttgagcaact atcaaatggt gagcggatag
     4381 atattcggat ttctccctat cttcaagatt tgtttgatgc cgcaagaact gatggagttt
     4441 acccgattgt cgcatccgga taccgaacaa cagaaaaaca gcaagaaatt atggatgaaa
     4501 aaattgccga atataaggcg aaaggctaca cctctgcaca ggctaaagcg gaagcagaaa
     4561 cttgggtggc cgtgccggga acgagcgagc atcagcttgg tcttgctgtg gatatcaatg
     4621 cggacggaat tcattcaaca ggcaacgagg tttatagatg gctggatgaa aacagctatc
     4681 gttttggttt tattcgtcgc tacccaccag acaagacaga gataaccggt gtgagcaacg
     4741 agccgtggca ttaccgatat gttggcatcg aagctgccac agagatgtac aaccaagggg
     4801 tttgccttga ggaatattta aaaccagaaa aatgagaaaa ggatgttatg ttatggacag
     4861 aaaaagattg acacagcgtt tcccgttcct gcttccaatg agacgggcgc agagaaaaat
     4921 gtgcttttat gcgggaatga gatttgacgg ctgtcgctat gcgcagacga taggggaaaa
     4981 atcgctttcc catttgctct ttgaaacgga ttgcgcatta tacaaccaca ataccggatt
     5041 tgacatgata taccaggaaa acaaggtgtt caacttaaag ctggcagcaa agaccttaaa
     5101 cggcttattg ataagaccgg gggaaacctt ttctttctgg ttgttcctgc atacgccatt
     5161 gacaattatc cagcgcagag gtcacgaagt aaaggagttt ccagagccaa acagtgacga
     5221 gattaaaggg gtggatgcaa ccatttcaga gggctggatt gatttaaaag tgcgaaacga
     5281 taccgactgc acctaccaaa tatgggtgac cttagatgat gagaaaatca tcggtcaggt
     5341 gttcgccgac aaagagcccc aagcattata caaaattaca aatggcagta tccagtatgt
     5401 ccgtgaaagt ggcgggattt atgaatatgc ccaggttaaa cggatgcaag ttgccttagg
     5461 taccggggaa ataatagatt gcaagctgct ttatacaaac aaatgcaaaa tctgttatcc
     5521 cctcccggaa agtgtggata ttcaggagga gaaccaatga gaaaaagtgt gggcattact
     5581 gtttttggat gcgaacagga tgaggcaaat gctttccgcg ctttatcgcc ggattttcat
     5641 attatcccta cgctgattag cgatgcgata tcggcagaca acgcaaaatt ggccgctggc
     5701 aatcaatgcg ttagcgtaag ccataagtcc gaggtttccg aggcgacaat tcttgcgctg
     5761 agaaaggtcg gggtaaaata catttctacc cgcagcatcg gctgcgatca cattgatacg
     5821 actgccgccg agagaatggg aatctcggtt ggcacggttg cgtattcgcc ggacagcgtt
     5881 gcggattatg ctttgatgct gatgctgatg gccatacggg gtgcaaaacc caccatgcac
     5941 gccgtggcgc aacaagattt cagattggat cgtatccggg ggaaagaact gggggatatg
     6001 actgtgggag ttattggaac cggccatatc gggcaagcgg tcgtcaaaag gctgcgggga
     6061 tttggatgcc atgtgctggc ctatgataac agccgaaaaa tggatgcaga ttatgtccag
     6121 cttgatgagc ttctaaaaaa cagcgatatt gttacgctcc atgtgccgct ttgtgcggat
     6181 acccgccatc tgatcggtca gaagcaaatt ggagagatga agcaaggcgc atttttaatc
     6241 aacaccgggc gcggagcgct tgtcgatacc ggggcgctgg tggaggcgtt ggaaagcgga
     6301 aagctgggtg gtgcggcact ggatgtgttg gagggcgagg atcagtttgt ttataccgat
     6361 tgctcgcaga aagtgcttga ccatcccttt ttgtcgcagc ttttaaggat gccgaatgtg
     6421 atcatcacac cccatacggc gtactacact gagcgtgtgc tgcaggatac tacagaaaaa
     6481 acaatcagga attgtctcaa ttttgaaagg agtttacagc atgaatagaa taaaagtcgc
     6541 aatcatcttc ggcggttgct cggaggaaca tgatgtgtcg gtaaaagccg caatagaaat
     6601 tgctgcgaac attgatacgg aaaaattcga tccgcactac atcggaatta caaaaaacgg
     6661 tgtatggaag ctatgcaaga agccatgtac ggaatgggaa gccgacagtc tccccgccat
     6721 actctccccg gataggaaaa cgcatgggct gcttgtcatg aaagaaagcg aatacgaaac
     6781 acggcgtatt gatgtggctt tcccggtttt gcatggcaaa tgcggggagg atggtgcgat
     6841 acaggggctg tttgtattgt ctggtatccc ctatgtgggc tgtgatattc aaagctccgc
     6901 agcttgcatg gacaaatcac tggcctacat tcttacaaaa aatgcgggca tcgccgttcc
     6961 cgaatttcaa atgattgata aaggtgacaa gccggaggcg ggtgcgctta cctaccctgt
     7021 ctttgtgaag ccggcacggt caggttcgtc ctttggcgta accaaagtaa acggtacgga
     7081 agaacttaac gctgcgatag aagcggcagg acaatatgat ggaaaaatct taattgagca
     7141 agcgatttcg ggctgtgagg tcgggtgtgc ggtcatgggg aacgaggatg atttgattgt
     7201 cggcgaagtg gatcaaatcc ggctgagcca cggtatcttc cgcatccatc aggaaaacga
     7261 gccggaaaaa ggctcagaaa atgcgatgat tacagttccc gcagacattc cggtcgagga
     7321 acgaaatcgg gtgcaggaaa cggcaaagaa agtatatcgg gtgcttggat gcagagggct
     7381 tgcccgtgtt gatctttttt tgcaggagga tggcggcatc gttctaaatg aggtcaatac
     7441 cctgcccggt tttacatcgt acagccgcta cccacgtatg atggccgccg caggaatcac
     7501 gcttcctgca ctgattgaca gcctgattac attggcgtta aagaggtgat aagtatggaa
     7561 aatggttttt tgtttttaga tgaaatgttg catggcgtcc gttgggatgc caagtacgcc
     7621 acatgggata acttcacagg aaaaccggtg gatgggtatg aggtgaatcg catcatcggc
     7681 acaaaggccg tggcgtttgc tctgcgcgag gcacaaatcc atgcggctgc gcttggctat
     7741 ggcttgcttt tatgggatgg atatcggcca agaactgcgg tggactgctt cctgcgttgg
     7801 gcagcgcaac cggaggacaa tctcacaaaa gaaaaatttt accccaatat agagcgagcc
     7861 gagttgatta caaagggtta tgtggcctca caatccagcc atagccgtgg aagcgcaatt
     7921 gatcttacgc tctaccacct ggatacaggg gaacttgttt caatgggaag taacttcgat
     7981 tttatggacg aacggtcgca tcatacagca aaagggatag gggatgcaga ggcacaaaat
     8041 cgaagatgct tgcgtaaaat catggaaagc agcggatttc agtcttatcg ctttgaatgg
     8101 tggcactata agttgattga tgagccatac cccgatacct attttaattt tgccgtttca
     8161 taatgaaagt attggttttg tatcagattt tggatttgat aaaatctatg ctggtcatgg
     8221 acaatctttt actggaaagt ggttaaagca tcttttgtaa tacattgcaa attatagtat
     8281 tcaatctgcc cagcggcaga aaagaaaaaa accgctgttg ggcagacctt tcccacgccc
     8341 attttgtatt ttgctggcgg cggttttgag aaatcaaggc cgccgttctt ttctggcagc
     8401 aaaaggtgtg gcggcccata ggaggatgcc gctatgctgt tttgctattg ccatgctcta
     8461 atccacttcc accactcaaa aaaagccttg cccctccatg aactgaaccc caaaagttga
     8521 actatttaat ggactgt
//
LOCUS       DAZBTF010000241         7943 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.guided.105, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000241 DAZBTF010000000
VERSION     DAZBTF010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.guided.105"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            128..1564
                     /locus_tag="ACHGNS_003071"
     CDS             128..1564
                     /locus_tag="ACHGNS_003071"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN6938911.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKSSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4185
                     /locus_tag="ACHGNS_003072"
     CDS             1753..4185
                     /locus_tag="ACHGNS_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFN6938912.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQLSFVPI
                     DSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQDYELPT
                     VDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEVQPAVG
                     VKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIEAQPNH
                     PDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRAKPHEV
                     KLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAATGVRNI
                     SGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMARAAGI
                     HMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGDMLFLP
                     MGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQPQDELF
                     EEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQE
                     ETSETLPDDNDM"
     gene            4415..5482
                     /gene="rlmN"
                     /locus_tag="ACHGNS_003073"
     CDS             4415..5482
                     /gene="rlmN"
                     /locus_tag="ACHGNS_003073"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFN6938913.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            5472..5678
                     /locus_tag="ACHGNS_003074"
     CDS             5472..5678
                     /locus_tag="ACHGNS_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938914.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(5675..6778)
                     /locus_tag="ACHGNS_003075"
     CDS             complement(5675..6778)
                     /locus_tag="ACHGNS_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFN6938915.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7330..7878
                     /gene="aac(6')"
                     /locus_tag="ACHGNS_003076"
     CDS             7330..7878
                     /gene="aac(6')"
                     /locus_tag="ACHGNS_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFN6938916.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2658 a         1357 c         1724 g         2204 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaagttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaata tgagaagaaa aaagcggtga gccagcaaag ggaaaatact
     2521 gctgatgaat ggcaggaacc agaaaataca gagccggagc agctaagttt tgttccaatc
     2581 gacagttttc aagaaaatat ccatcctgcg aatctagaga aaccagtacc agatacacca
     2641 aaacagacca atacagcaga aggtttttca gatgagttgc cagaggatga cggtacctca
     2701 ttagaatttg agattgaagc agaacaggaa aatcaagact atgaattacc gacagttgat
     2761 ttgcttgatt ctataccaac tgtggatcaa agtgatgagt ataaaaaaat tgaaaaaaat
     2821 atcggcgtat tagaacaaac tttccaaagt ttcggtgtcg atgccaaggt cgtcaaagcc
     2881 agcttaggac catccgtcac aaaattcgaa gtccagccgg ctgtaggggt aaaagtaagt
     2941 aaaattgtga acttaacaga tgatatcgcg cttgctttag cggcgaaaga tgtccggatg
     3001 gaagcaccga ttccaggtaa atcactgatt ggtattgaag taccaaatgg aaagatcagt
     3061 atggtttcct ttagagaaat catcgaagca caaccaaacc acccagataa attactagaa
     3121 gtacctttag gacgtgatgt ttctggacgg gtacaaacgg ctgatctttc gaagatgcct
     3181 cacttactcg tggctggttc gactggtagt gggaagtcag tagcaattaa tgggattatc
     3241 acaagtatat taatgcgtgc taagccccat gaagtaaaac tgatgatgat cgatccgaaa
     3301 atggttgaat tgaatatgta caacggcata cctcatctgt tgacgccggt cgtgacgaac
     3361 cctagaaaag cagcgcaagc cttacaaaaa gtagtacaag aaatggaaga acgatatgaa
     3421 aaattcgcgg caaccggagt aagaaatatt tctggttaca atgaatttgt tcaacagaaa
     3481 aacttggaaa acggaacgaa acatccaacc ttaccattca tcgtagtgat cgtagatgaa
     3541 ttagctgatt tgatgatggt agccagcaat gaagttgaag atgcgattat tcgtttagcg
     3601 caaatggctc gggcagcggg gattcatatg atcctagcta ctcaacgtcc aagtgtggat
     3661 gttatcacag gaatcatcaa agcaaatgta ccttccagaa tggcatttgc ggtatcaagc
     3721 ggtacagatt ctcggacgat cattgattct aatggggcag aaaagctctt agggcgagga
     3781 gatatgctat tcttgccaat gggtgagaat aaaccgatcc gtgtgcaagg agcattcatt
     3841 tctgaccacg aagtagaacg ggtcgttcaa tttgttacag atcagcaaga agctcattat
     3901 gaagagaaaa tgatgccgac agatgaagtt gaaacgacag gtgctccaga acagccacaa
     3961 gatgagctgt ttgaagaagc aaaagcactt gtcgttgaaa tgcaaacggc aagtatttcc
     4021 cttctccaaa gaagatttcg gatcggttac aaccgagcag cacgtttagt agatgaactg
     4081 gaagctcatg gagttgttgg cccgtcagaa ggaagcaagc ctcggaaagt attcattgag
     4141 caggaagaaa catcagaaac gttgccggac gataatgaca tgtaaaactt gtgtgatgaa
     4201 gggttgaaaa acgaataacc aataaaaaga caaggcatga tgataccaaa agaaaaaatg
     4261 gtttttttcc atcttttcta atacggtatg atgtcttgtc tttttagttt ttgtgagatt
     4321 aaagttctat tcgatttatt tgtgaagtat tgcattaagg aaatgaatca tgtatagtat
     4381 gtagcgaaac tttgagaaaa agagggaaaa tactttggaa aaacagtcca tttatggctt
     4441 gacgaatgaa gaattaatca attggtttat agaaaacggc gagaaaaaat tccgagctgc
     4501 ccaagtttgg gaatggttgt atcaaaaacg tgtaagtaat ttcacagaaa tgacgaatct
     4561 atcgaaacaa ttgatcgaaa aattatcgga gcattttatc atcaatcctc taaaacagat
     4621 ggtcgtccaa gaagcttcgg atggtacagt aaaatatttg tttgaactcc ctgataaaaa
     4681 catgattgag actgtactga tgagacaaga atatggcttg tcagtttgtg tgacgacaca
     4741 agtaggatgt aatatcggct gtacgttttg tgcaagcggg ctgctcaaaa agaaccgtga
     4801 tctgactgct ggagaaattg ttgcacagat catgatggtc caacattatt ttgatgagcg
     4861 caatcttggc gaacgcgttt cacatgtcgt tgtaatgggg atcggtgagc catttgataa
     4921 ttacgacaat gtaatggact ttttgcatat catcaatgat gccaaaggat tggcaattgg
     4981 tgctcggcac attacagttt ccactagtgg attagcacac aaaatcaagg aattcgctaa
     5041 taacggcttg caagtcaatc tggctatttc cttgcacgcg ccgaataatg aggtgcgaac
     5101 atcgatcatg cggatcaacc gaagtttccc aattgagaaa ttgatggaag ctgtggatga
     5161 gtatttagag aaaacaaacc gtcgaatcac gtttgaatat atcatgttga atcaagtaaa
     5221 tgatagacca gagcatgcgc aacaattagc agatctatta aaagataaga aaaaattagc
     5281 atatgtcaac ttgattccat ataatccagt cagtgaacat gatcaatatt ctcgcagtcc
     5341 aaaagctgat gtattgagat tttatgatgt attgaagaaa aacggcgtaa actgtgtgat
     5401 acgtaaagaa catggtacag atattgatgc agcttgcgga caattgcgaa gcaagcaaat
     5461 gaaaaaaaca gttgccaaat aatcgtatcc tactcgtttt ttcaattacc ataaaaggag
     5521 cagttttctt aattaaggaa ctgttcaact taaataagag aaaagattct atagcgaaag
     5581 ctataaagct ttttttgaca agaaaaaccc tcatcaagca aagaaatgtc ctcaatttga
     5641 tgacagcctg cttgatgggg gttttagcaa ttatttaact gattttctta tatccttgct
     5701 ggctttcttg tgtagcatga taagtttcta cgtcaagaat ggcctgttta aatttttctc
     5761 gggtgatttc ataaggcaca tgtttaatat catttgttgc catggcttta tctaagattg
     5821 cttctaaatc ggtcgtgaca ttgagattca tgtcggttaa cgatactgga agttggttct
     5881 ttttcataaa cacatagatt ttatcaaaca aatcgaactg ctgatccatc atcaataagg
     5941 tcagtacacc ataactgaca gatgctccgt gaaggtatcc ctccattttt tctagaacag
     6001 tcgtaccgta atagaatgag tgtgccatag cagaattata gttattgtta actaaaacag
     6061 aaaccaatcc ggtagtaaca atgatatccg aaacgacttg ttcaattgca tatgagggac
     6121 gatcgtcggc actatctttc aatgcttgcg taccataagt caagagacga tcaatgcaca
     6181 tttttccgat ttggattcct aatctatccg tgtagcctag atcatcgcca cgagcagaaa
     6241 aagtagattc gacttctttg gataaagcat cgccaatacc tgcccataag tatttatcag
     6301 gtgcttcggc aataattgct gtatcaatga aggtgtgtac aggtggtttt gaaggaaaga
     6361 agagtccgtc cattacgcct tcttcgttat agatgacaca taccgcagtg acgggcgaac
     6421 agttcgaacc aatagtggga aaggtgaaga ctggtttagc tagtttttca ccaatgactt
     6481 ttacagtatc gcaagcacgg ccaccaccga ctgcaaacag gacgtctgct ttttggacac
     6541 tttcatgctg gatcaactgt tcaatatttc cataagttga attacccccg taccagagac
     6601 tggcgacaac ctctatttcc tgtgtcaaag ctacttttat tttgttttct gcctttgaaa
     6661 gtgcagtctt ccctccaatc agcactactt ttgtcccata ttctttgacg attccatttg
     6721 ctttttcata cgcatctgtt ccaatggtat aatttggtaa gaaaacactt tgactcatat
     6781 tcatttcctt ctttcttcgt tgaaacgggt taaaatttta aagagtgatc gatctgactg
     6841 cggctgtaga tggagtagag gtgaacctaa gacaggataa gtgatgaata aggtggcatg
     6901 aacaccttga aatcatcaat aagtatccta aaaacgaaca agtacaaaaa cattgaaaac
     6961 gacaataatg aatttttgtt cctcttagta tttctaccga ttccctttgc ttttttgtaa
     7021 ttatctaaaa attctaaaaa ttaagattat tatatgaaaa caggttttat ttgtcaattc
     7081 ataattggtt ttttttaaag caaaagataa tctatatcat gatgaacaaa cagagttttt
     7141 tcctccaatt gatggctttc ctgatctttc ctattttgaa taaagtgttt ctgtataatt
     7201 ttttatttta taagtaagat aaataatgaa agaatagaga tactgattgg tagctaaaga
     7261 tttttgagcg agaacatttc gttttttcaa aaggactgtt agaataagca agaacaaggg
     7321 ggaagacgta tgataatcag tgaatttgac cgtaataatc cagtattgaa agatcagctt
     7381 tctgatttac tgagactgac ttggccggaa gaatatggag acagctcggc agaagaagta
     7441 gaagaaatga tgaatccaga acgaatcgcg gtagcagcgg tagaccaaga tgagttagta
     7501 ggatttattg gtgcaatccc tcaatacggt atcacaggtt gggaattgca tccattagtt
     7561 gtagaaagct cccgacgaaa gaaccaaata ggtactcgat tagtcaatta cttagaaaaa
     7621 gaagtagctt ccagaggagg aatcacgatt tatttaggta cggatgattt agaccatgga
     7681 acaacgttaa gtcaaacgga cctgtatgag catacatttg ataaagtggc ttccatccag
     7741 aaccttcgtg aacatccgta tgaattctat gaaaaattag gttataaaat cgtaggtgtc
     7801 ttaccaaatg caaatggctg ggacaaaccg gatatttgga tggcaaaaac gattattcct
     7861 cgaccaaatt ctcaataata aaaagaggtt gtgacagaag taatcagctt caagaaataa
     7921 gaaggaattt acgaaaatta ctt
//
LOCUS       DAZBTF010000242         7943 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.guided.106, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000242 DAZBTF010000000
VERSION     DAZBTF010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.guided.106"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            128..1564
                     /locus_tag="ACHGNS_003077"
     CDS             128..1564
                     /locus_tag="ACHGNS_003077"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN6938917.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKSSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4185
                     /locus_tag="ACHGNS_003078"
     CDS             1753..4185
                     /locus_tag="ACHGNS_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFN6938918.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQLSFVPI
                     DSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQDYELPT
                     VDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEVQPAVG
                     VKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIEAQPNH
                     PDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRAKPHEV
                     KLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAATGVRNI
                     SGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMARAAGI
                     HMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGDMLFLP
                     MGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQPQDELF
                     EEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQE
                     ETSETLPDDNDM"
     gene            4415..5482
                     /gene="rlmN"
                     /locus_tag="ACHGNS_003079"
     CDS             4415..5482
                     /gene="rlmN"
                     /locus_tag="ACHGNS_003079"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFN6938919.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            5472..5678
                     /locus_tag="ACHGNS_003080"
     CDS             5472..5678
                     /locus_tag="ACHGNS_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938920.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(5675..6778)
                     /locus_tag="ACHGNS_003081"
     CDS             complement(5675..6778)
                     /locus_tag="ACHGNS_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFN6938921.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7330..7878
                     /gene="aac(6')"
                     /locus_tag="ACHGNS_003082"
     CDS             7330..7878
                     /gene="aac(6')"
                     /locus_tag="ACHGNS_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFN6938922.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKFVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2657 a         1357 c         1724 g         2205 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaagttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaata tgagaagaaa aaagcggtga gccagcaaag ggaaaatact
     2521 gctgatgaat ggcaggaacc agaaaataca gagccggagc agctaagttt tgttccaatc
     2581 gacagttttc aagaaaatat ccatcctgcg aatctagaga aaccagtacc agatacacca
     2641 aaacagacca atacagcaga aggtttttca gatgagttgc cagaggatga cggtacctca
     2701 ttagaatttg agattgaagc agaacaggaa aatcaagact atgaattacc gacagttgat
     2761 ttgcttgatt ctataccaac tgtggatcaa agtgatgagt ataaaaaaat tgaaaaaaat
     2821 atcggcgtat tagaacaaac tttccaaagt ttcggtgtcg atgccaaggt cgtcaaagcc
     2881 agcttaggac catccgtcac aaaattcgaa gtccagccgg ctgtaggggt aaaagtaagt
     2941 aaaattgtga acttaacaga tgatatcgcg cttgctttag cggcgaaaga tgtccggatg
     3001 gaagcaccga ttccaggtaa atcactgatt ggtattgaag taccaaatgg aaagatcagt
     3061 atggtttcct ttagagaaat catcgaagca caaccaaacc acccagataa attactagaa
     3121 gtacctttag gacgtgatgt ttctggacgg gtacaaacgg ctgatctttc gaagatgcct
     3181 cacttactcg tggctggttc gactggtagt gggaagtcag tagcaattaa tgggattatc
     3241 acaagtatat taatgcgtgc taagccccat gaagtaaaac tgatgatgat cgatccgaaa
     3301 atggttgaat tgaatatgta caacggcata cctcatctgt tgacgccggt cgtgacgaac
     3361 cctagaaaag cagcgcaagc cttacaaaaa gtagtacaag aaatggaaga acgatatgaa
     3421 aaattcgcgg caaccggagt aagaaatatt tctggttaca atgaatttgt tcaacagaaa
     3481 aacttggaaa acggaacgaa acatccaacc ttaccattca tcgtagtgat cgtagatgaa
     3541 ttagctgatt tgatgatggt agccagcaat gaagttgaag atgcgattat tcgtttagcg
     3601 caaatggctc gggcagcggg gattcatatg atcctagcta ctcaacgtcc aagtgtggat
     3661 gttatcacag gaatcatcaa agcaaatgta ccttccagaa tggcatttgc ggtatcaagc
     3721 ggtacagatt ctcggacgat cattgattct aatggggcag aaaagctctt agggcgagga
     3781 gatatgctat tcttgccaat gggtgagaat aaaccgatcc gtgtgcaagg agcattcatt
     3841 tctgaccacg aagtagaacg ggtcgttcaa tttgttacag atcagcaaga agctcattat
     3901 gaagagaaaa tgatgccgac agatgaagtt gaaacgacag gtgctccaga acagccacaa
     3961 gatgagctgt ttgaagaagc aaaagcactt gtcgttgaaa tgcaaacggc aagtatttcc
     4021 cttctccaaa gaagatttcg gatcggttac aaccgagcag cacgtttagt agatgaactg
     4081 gaagctcatg gagttgttgg cccgtcagaa ggaagcaagc ctcggaaagt attcattgag
     4141 caggaagaaa catcagaaac gttgccggac gataatgaca tgtaaaactt gtgtgatgaa
     4201 gggttgaaaa acgaataacc aataaaaaga caaggcatga tgataccaaa agaaaaaatg
     4261 gtttttttcc atcttttcta atacggtatg atgtcttgtc tttttagttt ttgtgagatt
     4321 aaagttctat tcgatttatt tgtgaagtat tgcattaagg aaatgaatca tgtatagtat
     4381 gtagcgaaac tttgagaaaa agagggaaaa tactttggaa aaacagtcca tttatggctt
     4441 gacgaatgaa gaattaatca attggtttat agaaaacggc gagaaaaaat tccgagctgc
     4501 ccaagtttgg gaatggttgt atcaaaaacg tgtaagtaat ttcacagaaa tgacgaatct
     4561 atcgaaacaa ttgatcgaaa aattatcgga gcattttatc atcaatcctc taaaacagat
     4621 ggtcgtccaa gaagcttcgg atggtacagt aaaatatttg tttgaactcc ctgataaaaa
     4681 catgattgag actgtactga tgagacaaga atatggcttg tcagtttgtg tgacgacaca
     4741 agtaggatgt aatatcggct gtacgttttg tgcaagcggg ctgctcaaaa agaaccgtga
     4801 tctgactgct ggagaaattg ttgcacagat catgatggtc caacattatt ttgatgagcg
     4861 caatcttggc gaacgcgttt cacatgtcgt tgtaatgggg atcggtgagc catttgataa
     4921 ttacgacaat gtaatggact ttttgcatat catcaatgat gccaaaggat tggcaattgg
     4981 tgctcggcac attacagttt ccactagtgg attagcacac aaaatcaagg aattcgctaa
     5041 taacggcttg caagtcaatc tggctatttc cttgcacgcg ccgaataatg aggtgcgaac
     5101 atcgatcatg cggatcaacc gaagtttccc aattgagaaa ttgatggaag ctgtggatga
     5161 gtatttagag aaaacaaacc gtcgaatcac gtttgaatat atcatgttga atcaagtaaa
     5221 tgatagacca gagcatgcgc aacaattagc agatctatta aaagataaga aaaaattagc
     5281 atatgtcaac ttgattccat ataatccagt cagtgaacat gatcaatatt ctcgcagtcc
     5341 aaaagctgat gtattgagat tttatgatgt attgaagaaa aacggcgtaa actgtgtgat
     5401 acgtaaagaa catggtacag atattgatgc agcttgcgga caattgcgaa gcaagcaaat
     5461 gaaaaaaaca gttgccaaat aatcgtatcc tactcgtttt ttcaattacc ataaaaggag
     5521 cagttttctt aattaaggaa ctgttcaact taaataagag aaaagattct atagcgaaag
     5581 ctataaagct ttttttgaca agaaaaaccc tcatcaagca aagaaatgtc ctcaatttga
     5641 tgacagcctg cttgatgggg gttttagcaa ttatttaact gattttctta tatccttgct
     5701 ggctttcttg tgtagcatga taagtttcta cgtcaagaat ggcctgttta aatttttctc
     5761 gggtgatttc ataaggcaca tgtttaatat catttgttgc catggcttta tctaagattg
     5821 cttctaaatc ggtcgtgaca ttgagattca tgtcggttaa cgatactgga agttggttct
     5881 ttttcataaa cacatagatt ttatcaaaca aatcgaactg ctgatccatc atcaataagg
     5941 tcagtacacc ataactgaca gatgctccgt gaaggtatcc ctccattttt tctagaacag
     6001 tcgtaccgta atagaatgag tgtgccatag cagaattata gttattgtta actaaaacag
     6061 aaaccaatcc ggtagtaaca atgatatccg aaacgacttg ttcaattgca tatgagggac
     6121 gatcgtcggc actatctttc aatgcttgcg taccataagt caagagacga tcaatgcaca
     6181 tttttccgat ttggattcct aatctatccg tgtagcctag atcatcgcca cgagcagaaa
     6241 aagtagattc gacttctttg gataaagcat cgccaatacc tgcccataag tatttatcag
     6301 gtgcttcggc aataattgct gtatcaatga aggtgtgtac aggtggtttt gaaggaaaga
     6361 agagtccgtc cattacgcct tcttcgttat agatgacaca taccgcagtg acgggcgaac
     6421 agttcgaacc aatagtggga aaggtgaaga ctggtttagc tagtttttca ccaatgactt
     6481 ttacagtatc gcaagcacgg ccaccaccga ctgcaaacag gacgtctgct ttttggacac
     6541 tttcatgctg gatcaactgt tcaatatttc cataagttga attacccccg taccagagac
     6601 tggcgacaac ctctatttcc tgtgtcaaag ctacttttat tttgttttct gcctttgaaa
     6661 gtgcagtctt ccctccaatc agcactactt ttgtcccata ttctttgacg attccatttg
     6721 ctttttcata cgcatctgtt ccaatggtat aatttggtaa gaaaacactt tgactcatat
     6781 tcatttcctt ctttcttcgt tgaaacgggt taaaatttta aagagtgatc gatctgactg
     6841 cggctgtaga tggagtagag gtgaacctaa gacaggataa gtgatgaata aggtggcatg
     6901 aacaccttga aatcatcaat aagtatccta aaaacgaaca agtacaaaaa cattgaaaac
     6961 gacaataatg aatttttgtt cctcttagta tttctaccga ttccctttgc ttttttgtaa
     7021 ttatctaaaa attctaaaaa ttaagattat tatatgaaaa caggttttat ttgtcaattc
     7081 ataattggtt ttttttaaag caaaagataa tctatatcat gatgaacaaa cagagttttt
     7141 tcctccaatt gatggctttc ctgatctttc ctattttgaa taaagtgttt ctgtataatt
     7201 ttttatttta taagtaagat aaataatgaa agaatagaga tactgattgg tagctaaaga
     7261 tttttgagcg agaacatttc gttttttcaa aaggactgtt agaataagca agaacaaggg
     7321 ggaagacgta tgataatcag tgaatttgac cgtaataatc cagtattgaa agatcagctt
     7381 tctgatttac tgagactgac ttggccggaa gaatatggag acagctcggc agaagaagta
     7441 gaagaaatga tgaatccaga acgaatcgcg gtagcagcgg tagaccaaga tgagttagta
     7501 ggatttattg gtgcaatccc tcaatacggt atcacaggtt gggaattgca tccattagtt
     7561 gtagaaagct cccgacgaaa gaaccaaata ggtactcgat tagtcaatta cttagaaaaa
     7621 gaagtagctt ccagaggagg aatcacgatt tatttaggta cggatgattt agaccatgga
     7681 acaacgttaa gtcaaacgga cctgtatgag catacatttg ataaagtggc ttccatccag
     7741 aaccttcgtg aacatccgta tgaattctat gaaaaattag gttataaatt cgtaggtgtc
     7801 ttaccaaatg caaatggctg ggacaaaccg gatatttgga tggcaaaaac gattattcct
     7861 cgaccaaatt ctcaataata aaaagaggtt gtgacagaag taatcagctt caagaaataa
     7921 gaaggaattt acgaaaatta ctt
//
LOCUS       DAZBTF010000243         7446 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.guided.110, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000243 DAZBTF010000000
VERSION     DAZBTF010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.guided.110"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..369
                     /locus_tag="ACHGNS_003083"
     CDS             <1..369
                     /locus_tag="ACHGNS_003083"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938923.1"
                     /translation="IFKTAVFNSNQSVLLTVSPQLLNNPRFLAMQIEQMYEIVVPEFV
                     EPVLEQGIKDKSLEIENSHEIAEVIMVLSNVWLNPLVEMTDKEGMEKRCNTFNALLNG
                     IGIDTLLDEETISAFIQFCK"
     gene            511..1713
                     /gene="mef(H)"
                     /locus_tag="ACHGNS_003084"
                     /gene_synonym="mefH"
     CDS             511..1713
                     /gene="mef(H)"
                     /locus_tag="ACHGNS_003084"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFN6938924.1"
                     /translation="MLVVIGQIISLFGNAILRFALPYYLLKETGSSALFGIATACSFL
                     PMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPIIPLFIVALMLLYG
                     ISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGVVFQFYGINPILI
                     VSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKKEKPIFISVIFLV
                     CIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGILTALVASKLKLK
                     NLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTMFVVQIYTLVQTQ
                     TPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVASIASIMISIYSK
                     RIFQKLEI"
     gene            1940..2359
                     /locus_tag="ACHGNS_003085"
     CDS             1940..2359
                     /locus_tag="ACHGNS_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFN6938925.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            2364..2603
                     /locus_tag="ACHGNS_003086"
     CDS             2364..2603
                     /locus_tag="ACHGNS_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6938926.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(2828..3751)
                     /locus_tag="ACHGNS_003087"
     CDS             complement(2828..3751)
                     /locus_tag="ACHGNS_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFN6938927.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(3783..4547)
                     /gene="map"
                     /locus_tag="ACHGNS_003088"
     CDS             complement(3783..4547)
                     /gene="map"
                     /locus_tag="ACHGNS_003088"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFN6938928.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4705..5145
                     /locus_tag="ACHGNS_003089"
     CDS             4705..5145
                     /locus_tag="ACHGNS_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFN6938929.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            complement(5323..5892)
                     /locus_tag="ACHGNS_003090"
     CDS             complement(5323..5892)
                     /locus_tag="ACHGNS_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN6938930.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            6147..7379
                     /locus_tag="ACHGNS_003091"
     CDS             6147..7379
                     /locus_tag="ACHGNS_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFN6938931.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
BASE COUNT         2365 a         1305 c         1380 g         2396 t
ORIGIN      
        1 atatttaaga cagcggtatt caattctaac caaagcgttt tattgacagt ttctcctcaa
       61 ctattaaaca atcctcgttt tcttgctatg caaatagaac aaatgtatga aattgtagta
      121 cctgaatttg ttgaacccgt tttagaacaa ggtatcaagg ataagtcttt ggagattgaa
      181 aattcacatg aaattgcaga agttatcatg gtattaagta atgtatggct caacccatta
      241 gttgaaatga cggataaaga gggtatggaa aaacgttgca atacattcaa tgccctttta
      301 aatggaatag gcatagacac attattagac gaagaaacaa tttctgcatt tatacagttt
      361 tgtaaataac tgtatttgtt atccctatac gatagactgc tctataaaag agcagtttat
      421 ttaaaccatt atataaaccg acggtctgtt tgattaaata aaaaggagag attttatgca
      481 agcagaaaaa aactttttaa tcgtgatttt atacttgttg ttataggaca gataatttct
      541 ttatttggaa acgctatcct acgatttgca ttaccgtact atttgttaaa agaaactggt
      601 tcatcagctt tgtttggtat tgctacagcc tgctcttttt tacctatggt tattttgtcg
      661 ctggtaggtg gtattattgc tgacaggaca aataaaagaa acattatggt catactagat
      721 tttgtaacag caatcataat tttagtattt tatttgtcat tgggaaaaat tcctatcata
      781 cctttgttta ttgttgcttt gatgttactt tatggtattt ctggtgcata tcaaccagcg
      841 gtacaagcaa gtattcctct attggtatca tcagataaac ttaccgtcgg taatgctata
      901 ataaatcaag taaatacttt agcaaacttt atcggaccag ctataagcgg agtagttttt
      961 caattttatg gtatcaatcc cattttaatt gttagtatta tatgttttac tttttcagcc
     1021 attatggaaa tttttatcca tattccacac caaaaaagac ctcgtgaagt aggtgtaatt
     1081 gcagttgcta aaaacgattt aaaagatagt tatacctttt tgaaaaagga gaaacccatt
     1141 tttatatccg taatcttttt ggtttgcatt ttcaatttgg ttttaagtgc tgttttaatt
     1201 gttggtctgc ctatcttaat ttctgaagtt ttaaaaatgc cagatacact atatggtttt
     1261 tcacaatcag cattatcatt aggtggttta tgtggtggta ttttaacagc tcttgttgct
     1321 tcaaagttaa aactaaaaaa cctttatacc ttgttactta tttgttctgt ttctgtttta
     1381 cttatgggaa tttcgctatt tttagaattg cctgcaatag tttcctactg ggtagttaca
     1441 gtgatgtgct ttgtggctat gggtgtatct actatgtttg ttgttcaaat ttacactctt
     1501 gttcagacac aaacaccacc gcaattagta ggaaagataa tggctacgct ggtagcagtt
     1561 gctatgtgcg gtcaacctat tggacaaatg gtttacggta ttttattcga tgtatttata
     1621 caagaaacat gggttgttat gtttgtagct tctattgctt caatcatgat ttcaatatat
     1681 tcaaaaagaa tttttcaaaa gttagagata taatacaagg ggttaaatag tatgttaggc
     1741 taaaaacaat atataaaaga aaatttgaaa tctgctaaaa ggatagtgtt ataaaagaaa
     1801 gagtaatatt gcagtattta aggctcgcac aaatatatgt tttgtgtggg cttttttctt
     1861 accatttttc tgtatttcta aagggtataa acggtattcc gtttatatat ttcagcatta
     1921 gcagaaaggg ggtgagatta tgaggacatc ttctttcgag catatcgtca gattacagtt
     1981 caattcttta atgctgattg tcattaagaa caaagtgaaa agtcgaaaca gacagcttgc
     2041 aagacattct aaacgtgaag tattgttttg tgaaatagcc gaaacgaaac aagttgaacg
     2101 tggaacgatt gatacctgtt tttatgattg tgtatcattt aaagtcttgc actttacaat
     2161 ttatgtatct gatgaaacac ttggtacagt tttacataga ctgtcagaaa aacagcgtag
     2221 tgcgatttta ttacgttatt ttcaaggaat gaacgacaga gaaatttcag aactgtacca
     2281 tgtatcacgt tctgctattt gcagtagaag aactagagga ctaaggaagt taaaaatgct
     2341 tttgagcgaa aggaagtaaa gaaatgagta aagattatgg ctatccctct tttgaattga
     2401 tttgcaaagc gtcaagcgga gatgaaatgg cagttaaaga gattttgaag ttttatgacg
     2461 gttatatttc taaattatgc ttgcgtcctt tttaccactc tgaaagtggt aaaatcatta
     2521 tgcaggttga tgaagaacta aaaggagaaa ttcatacaga catgatgaaa gcaatattga
     2581 aatttgaaat cggagtgaag taatcaactg tataaaaaaa tcgggagata cacagtttcg
     2641 tgctatctcc cttagtagtt ttacggtcat atcaaggctc attcaatcgt ggtaaattcg
     2701 tggtaagatg agtgtttttc tatacttcaa aatgcttgaa tgtatagtgt ttatgcggat
     2761 aattgaaaat tagatataaa atttcaaata aattactgat ataaatttac aataagaact
     2821 cttgtttcta ctttttttta tgagaaaatt tagttttcaa tttattgaac cattttcggc
     2881 ttggatctcg acgttttttg atttgatgac ccgtcacgta ttcttgaaca actgcattga
     2941 tgattccgcc caaaatgatg atcgtagcag caaaattaag ccaaagcatg agtacaataa
     3001 aacttcctat gatctgataa ccgctgatcc tcgttgcaaa atacctagca tagatactaa
     3061 acacttgtga taacagcatc cagccgacag tagcgaagat cgtgcccgga atgatcgtct
     3121 tcaacttcaa ctgagcatta gggacaatta aataaatcaa gaacatgatg acaaataaag
     3181 ctaccaatgt gattggccac ttcaaagttt ggaactgatc gataaagttc acgggtattt
     3241 gtacgattgg ctgaatagca tccaaaatca gctgtcccaa cccaagaacg atagttaccc
     3301 cactaatagc aatcatgaac aagacaatga caattagtga gaagaaacgg acgatcaaaa
     3361 aattttttct attttctacg ccatacgctt tattcatcgc aatttgcaaa gcattgatac
     3421 tttgacttgc agaccataaa gccgctaatg cagaaacgga taacaagcct cctgaacttt
     3481 gtgtcaaaag gtctttgatt gcaggaccga taaactggta aacggtctca ggaatcactt
     3541 ctcgtaaata ggtcagtaca gtctcttgat cgatgtggag aaacggtaaa atattaccaa
     3601 aagcaataat caatggaaat aaagacaaga gtaaataata agcaacgacg acagaggtcg
     3661 tactcatttc tgaatcagtg atgcgctttt gagtcgtctc aataaagcgc atcagatttt
     3721 cattttgttt tattttgttc caaactttca tgtacctctc ccgtacctat tgactggata
     3781 gatcaataag tgccttcttc tccttgtgat gtcaaaattc ttggaccatc tttagtaata
     3841 gccaatgtat gttcatattg gcaagaaagt ccgccgtctc ttgtatacgc agtccagccg
     3901 ttaggatcca ttttcatttt ccaagttccg gtattaacca taggttcgat cgtgataacc
     3961 atgccttctt tcaaccgcaa cccttttcca gcttctccgt aatgaggaac tgctggttct
     4021 tcatgaatcg taggaccaac gccatgacca ataaaatcac gaacgatcgc tagattttcg
     4081 ccttctacat aagtttggat agcatgaccg atgtcaccaa tccgattccc tacttgtgct
     4141 tgctcaatac cgatgtacaa agctttcttc gttacttcca tcaatcggtc gatctcaggt
     4201 gtagattctc caactacata ggcccaacaa gaatcagaaa tgccgccttt caaatcgata
     4261 cacatatcga cttttaccaa atcaccgttt ttcaaaggct ttttacgagg gaatccatga
     4321 caaatttcat catttatact gatacaagtc gcatattcat acccttcaaa gccaatttgc
     4381 gcagcaattc cgccatgact ctcgatatag tttctgacaa atacctcaat atcccaactt
     4441 gtgattcctg gttttataaa atcacgcaga tttttatgta catctgctaa taaagctcca
     4501 gattctgcca tcatttcaat ttcacgtgct gattttaaag taatcatatt ttcgtctcct
     4561 ttttacaaat ccctctcatt ttaacatagc ggcatacatt tagccaaatg acattcccca
     4621 ggtgtctcta ggttttattt tcttttcaat gatgttttgg tataattcat aattgttaaa
     4681 taattataaa cgaggaggca catcatggct ttagcaaaaa tcgtatatgc tagcatgaca
     4741 ggaaacactg aagaaatcgc cgatattgtt gcagaagcat ttgaaaatct ggatatcgaa
     4801 gtcgaaatca acgaatgtac ccaagtagat gcagatgaat tcgaggaggc agatatttgc
     4861 gtagtagcta cttatacata cgatgatggc gatctgcctg atgagatcgt agacttttat
     4921 gaagacttgc aagaattgga tctattggga aaaatttacg gtgtctgcgg atctggtgat
     4981 actttttacg atgaattctg caaatcggtg gatgatttcg atgctgcttt tgcaaaaaca
     5041 ggcgcatcaa aaggagccga aaatgttaaa gtggatctga atgctgaaga agaagatatc
     5101 gaaaatttag aagctttcgc taaagaacta gtcgcaaaaa tttaatatac ttgcaataaa
     5161 agagccatcc agatttattc aatctgtatg gctcttttat tcattgcatt catggaagag
     5221 ttcttcggga gttaaaaatg catacgctgt aagaaaaacg aacttttcct tattttgaca
     5281 catctcttgt tacctactga tttgtggaac ttttgagtat attcaatcga tttcgattgg
     5341 ttctttgaat ttcaagatag catctatact gtcttggatc atcttttcaa tctgttcatc
     5401 agttggatgg atgatgcttg aagacatcaa agctgcgatc ccagtagcta tgatccatgt
     5461 cccattatac aaagattcga tctgctcttc tgaaagattc acataagctg gatcgcgttt
     5521 cactgtattc acaaagtaag tataagagaa attctgcatc tcttttcctc caccatattc
     5581 ttctaagtac agtgaacgat acaacttctt ctctcttctt gcaaaatgga tataattaaa
     5641 agcaagatcg ataatcgtat tccctgtatg ctcaacagtg aacacttcat attgtaaatg
     5701 atcatagatc tgctcaaaaa gagcttgtct caagtcatcc atatttttaa actcaagata
     5761 aatcggttgc gtggaacaat tcattttgtt cgcgatgttt ctcgcagtaa atcgtgtaaa
     5821 tccatcggtg gcagctactt cataagcagc tgccaaaatc tgttctctag taatcgtctt
     5881 ttttcttgcc atatttttta atctccttaa tttttttata acgcttccac cattgattaa
     5941 tttcattgta acatgttttt tttgatatgc aaaattagtt tttgattttc ttcacaagct
     6001 gaaatagcaa gtagtaacat atatcatgaa acttaacctt aaaaaaataa cctccttttt
     6061 ttatttaaag cctcattgtt tgaatcataa ttaaatggta tcatgctaat agttaccaga
     6121 aaaaatggtg aggaggaatt tttattatgt tatctgattt taatgaactt ctaaaaaaat
     6181 atgcacgctt aattgcagaa actggcgtag ccacagaaaa aggacacaca gttgttttac
     6241 agatcagcgt ggaacaggca ccccttgctc gattgatcac acaagaagct tataaactag
     6301 gagccgctga ggttatcgtt caatggactg acgaccagat ccaaagagaa tttcttttac
     6361 atgctgcaac ggatcgtatt gagaatgtac cacaatctaa aatcgatcaa gcggatgaat
     6421 ggcttgaaaa aggagcaagc aggatcagcg ttgtttctgc cgatcccgat gcatttgctg
     6481 gtgtagatag ccatcgagta gcgtcttatc aatcagcgaa tggcaaagca ctaatgaatt
     6541 tacgcaaggc aactcaagca aataaagtca gttggactgt cgtggctgcg gctggtaaac
     6601 aatgggcagc aaaagtcttc ccagaactgc cagaagaaaa acaagttgag gcattatggg
     6661 atcagatttt caaaacgact cgtgtctatg aagaagaccc tgtcttagca tggaagaagc
     6721 atgatgaaaa attagcaaaa aaagcagaag aattgaatcg agaacaattt tctgcccttc
     6781 actatactgc tcccggtaca gatatcatta tcggattgcc taaaaaccat ctttgggaag
     6841 gcgctggaag ctataatgct cgtggagaaa aattcatggc aaatatgccg acggaagaag
     6901 tgttcacagc acctgacagt catcgagtag atggttacat ttcaagtaca aagccactga
     6961 gttatgctgg caccatcatc tcgggtatga aatttacttt taaagatgga aaagttgttg
     7021 atttttctgc cgaacaagga gaagatgtct tagcaaaact gcttgataca gatgaaggtg
     7081 cgcgtcgttt aggagaagtc gcacttgtac ctgacccttc acctatttct caatctggca
     7141 tcatcttttt caatacatta tttgatgaaa acgcatcgaa tcacttagca ctaggttctg
     7201 cttatgcctt cagtgtgaaa ggcggtacag aaatgtccga tgaagaatta gcagaagctg
     7261 gattgaatag aagtcaaacc cacgtagatt tcatggtagg atctgacaag atggatatcg
     7321 acggaatccg tgaagatggc tctacagtac cgattttccg aaatggcgat tgggcttgag
     7381 tatactttcg cacacatgca aaaaataatg gctctttgtc aataaggact gatgagttgt
     7441 gcaaaa
//
LOCUS       DAZBTF010000244         5268 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.guided.129, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000244 DAZBTF010000000
VERSION     DAZBTF010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.guided.129"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            172..864
                     /locus_tag="ACHGNS_003092"
     CDS             172..864
                     /locus_tag="ACHGNS_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFN6938932.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSWKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1191..1688
                     /gene="dfrG"
                     /locus_tag="ACHGNS_003093"
     CDS             1191..1688
                     /gene="dfrG"
                     /locus_tag="ACHGNS_003093"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFN6938933.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1760..3712)
                     /locus_tag="ACHGNS_003094"
     CDS             complement(1760..3712)
                     /locus_tag="ACHGNS_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFN6938934.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3719..3955)
                     /locus_tag="ACHGNS_003095"
     CDS             complement(3719..3955)
                     /locus_tag="ACHGNS_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938935.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            4357..4647
                     /locus_tag="ACHGNS_003096"
     CDS             4357..4647
                     /locus_tag="ACHGNS_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6938936.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(4919..5268)
                     /locus_tag="ACHGNS_003097"
                     /pseudo
     CDS             complement(4919..5268)
                     /locus_tag="ACHGNS_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324676.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
BASE COUNT         1780 a          867 c          915 g         1706 t
ORIGIN      
        1 ctgcattttt ctctataaaa aagatttttt aaatagataa aaaaggagga ttaaatgagt
       61 atttgatagt atttatatta aaattacata tgtaatttaa aaacgcttga cattgtgttt
      121 gagatcactt acttttagca tgtaaaagta ttggattaag gagctatgca aatgaaaaag
      181 aaatttatta ctggtgggat tgtcgttgca tttctatgtc taattggcta cacaggcacg
      241 cggtacgcaa atgaaattat tttgtggggt ggagagacag atattgatac aatcaatgag
      301 aatctgacac agcttgatca tacattgaca gataaagagc aagcgattac agatcttacc
      361 gctcgactag cacaaaaaga acagacattg caagattcaa aggcagatgt ccaagaatac
      421 caaacaaaag taaatgaatt agaaaaccaa aaaaatcaat cggcagcaga aaaacaaaac
      481 ttagaaagtc aattagtcag caaggatgca caactacaaa gcaaacaaaa tgaaatcaat
      541 gtcaaactag atgagatcaa ccaaaaaaat gatgaaatca atcatctgaa tgcagattgg
      601 acagcaagac tacaagaagc acaagcaaaa ctttcagaat tacaaaatac tgtaaatggt
      661 ttgaatcaac aaatcgcaac actgacacaa gaaaaaacaa atgtagaaac tcagttaaca
      721 gatacacaaa agaaattaga cgaagcaact gctgaaaact caagctggaa acaatatatt
      781 caaaagttag aaaaagcaaa agatgaagtg gaagatacag ctgataaatc tcagcaaatt
      841 gttgaagaac atttgcgtaa ataatctttc ttaacaagta aacctaaatt agtaaagaag
      901 caacagtaat ctatactaag tagttcagtt ttggagtaca aaatgacaaa atgtacttca
      961 aaatggacta cttttttcat tttaacttac ataccaaatt acatacaaaa tgatttataa
     1021 aatgcaattg aaaatgataa ttgaatgaaa atttgtgatt ttgtggtatt atgataatat
     1081 aacttataga aaaaattgct tattcaacta acgggcagga taatgagaaa aggttttttg
     1141 atagaaataa gaaattagat ggtaaattag ttaaaaaggc tgtgttataa atgaaagttt
     1201 ctttgattgc tgcgatggat aagaatagag tgattggcaa agagaatgac attccttgga
     1261 ggattcccaa ggactgggaa tatgttaaaa atactacaaa gggacatccg ataatattag
     1321 gtaggaagaa ccttgaatca atcggaagag ccttacctga cagaagaaat attattctga
     1381 cgagagataa ggggtttacc tttaatggtt gtgaaattgt tcattcaata gaagatgttt
     1441 ttgagttatg taaaaacgaa gaagaaattt ttattttcgg aggagaacag atttataatt
     1501 tgtttttccc ttatgttgag aaaatgtaca tcacaaaaat acatcatgaa ttcgaaggag
     1561 atactttttt tccagaagtg aattatgagg aatggaatga ggtatttgcc caaaaaggga
     1621 taaagaatga taaaaatccg tataactact attttcatgt atatgaaaga aaaaacttat
     1681 tgagttaacg ggtgcgttaa ttgaagaaga acagtaaaga taaagcacaa gagcttggga
     1741 ttactccaag ctctttttgt catttgaaat aaattatatc tttccaatca aattgtttat
     1801 ctgtaatccc taatctttga gcaggtgtca aaaccttttt atccgctgat ttaaaagggc
     1861 gacaaaagtt gtagtaagtt cttagaattg taatcgcaaa ttgagcatac ttcggattga
     1921 agttagcgta aatatagctc ttcttatcac cacgagctgt tgttaatggt ctttcaagag
     1981 atgatatata acgtcttatc tgttgaataa agctattggt cgaatggtca ttaacattta
     2041 atagcatctt tgcgatttcg tttggttcca gagcagataa atccgttcta caatcaactg
     2101 aataaaatcc tttatcctta gtggctaatg gatgtttgat agggttatcg gcatatttgc
     2161 gatatctttc tccatcttta ctaatagcct cttcgtggaa ttgatgactt gttagtaatt
     2221 cagttagata atgagatgcc aaagtccgta gaaactttgt tttgaatcct ctgatatctc
     2281 cccaatcgag cagttctgct tttgcttgtc cgaattcttt taagcattgt tttctgcttt
     2341 tcgtcttgtt aacttggcaa ataaaatgat gtgcatcgct caaccttaat tctttcgtga
     2401 atactctata aaaggcgttt ctaatagaga agtcatcatc actaatcatt cgccattcgt
     2461 cggcattaac catttgttta attaaccaat agtgagcagt tgtagtataa ggagcatcaa
     2521 catttaaacc atcgacaaat ctactcctat tagtaatttt ccctaattcg tgaagataag
     2581 cattcctatt ttcgctatcg ttagcggatg gttcttgtgg ataataagac caatctaacc
     2641 tatcattttt acgactaaaa gtattgagat ggtcttcttt gaattttctt gtatcttcat
     2701 ttaattcatc cattgaaata ttccaatcat aggcaacatc agaacggaac acatatcttg
     2761 ataagacctc agcagataca actatatacg tttgcatatt aaggtcttca aaccccgtat
     2821 atttctttga accttgtcct ttcttacgga cgttatttag gtagtagtgc atcttgtccg
     2881 tattaagcca catctcgtta aatttctttg tttgaagggg ttgtgtctca tatcgttcta
     2941 aaaactccaa acaacgccta tacagccact ccagcttatg atagtaggtt ccaactccaa
     3001 ttcctaaaat atcacaagta cgactgactg aaacccttcc aacaaccatc ttagcgaaca
     3061 ttggaagaat ggtgttcttt tgttggtgat aagtagtggt ttcttctctt tttggtagaa
     3121 cattagtgaa cttcttacac gccttacatt gatagcgttg agatttgccc ctacttttcc
     3181 cccttttgta aaattgcttc ggctcattga atggggttga ttcttcttcc gagcagcctt
     3241 ctttatgaaa ttgataatct ggctcaacat cttggatgct attgatttct ataaggcgtt
     3301 taatttcttc aacaacagac caattagata aaggggttac tgtacagttt tgcgatacac
     3361 ctctgccaat aggattgtca ttgcagtaca tacctttatg acctttatct ttggaactac
     3421 cagtcatttt atatcgactt ggtttccctt ttacatcttt gaatttgtgc tgttctttcc
     3481 caaaattaca acaaaacgga ttgatgcagt aattatattg aatgggatat tcagaaccgt
     3541 tccatttaaa agacgttggc aagaacaaca ttttgttata ccttgttgca aatcgttttg
     3601 gagttaataa atcatattgt ttagccctat cttttaattc ttcgtttgaa atcggaattt
     3661 taacatctac aatttcatct tccttcgtgg ctaatctttt aagcttggtc attgtctatc
     3721 aacccactct atctttgata cccatttttt caacaatccg cttattactt cgcttgttgg
     3781 caatccattc aataaacttc ttatcgtcta aaatgttcaa taagaattca tccactgctt
     3841 cgctttcagt acgctctgca tattcagcgt attgcttaac tatcaccctt acttgctctg
     3901 atatttccca atccacagac ttagcgttct tattttttgg ctcaagaaat tgcataggaa
     3961 aatgcacccc tttttgctta gtaaattgta accattttaa cacaccaatt tgcattccta
     4021 caataattat ttcacgttcg catttttata tacattttaa cttacatttt gtggtgtaaa
     4081 ttaacaacaa acgttgattt aacaataaaa aacgagtatc aaattcagtt gcaatttgat
     4141 actcatttag gtttacattt tcactttttg tactccaaaa ctgaactact taaatctata
     4201 ctgctgcctc ttttttttag tacatgtcct tcatcttcgc aggaagtgcc aatatgattt
     4261 taagaaaggt aatagacaaa aagtcaaaaa gataaaacag agtttcttct ttacaaaggg
     4321 aatgtatgtt cgtataatgg tattgaggtg aaggttatgg agaaaaaata tggaacagta
     4381 gataagatcc gtactttgca agttagtgcc agaccgttga tccgtttcag tttaaatggg
     4441 gtcaattgtt tgattgcttc ccatagtttg aacttcctag ctgaagtaga cgaagggatg
     4501 aagttagtcg ttacaggtta ttataataat agaaagcaat ttgttgtgag atcctatcat
     4561 gtgttaggga aacccaaaat tgtggtagaa tatgagaaaa gtctataccc aagaaaaaag
     4621 gtccgacaag atgaaagaat aaactagaaa acaagcggct gagacaaaaa tgtcctagcc
     4681 gcttgttttg gaaagatgga tgcagacgat gggatttgaa cccacacgag catacgctca
     4741 caagaacctc aatctatata taactacatg tatttaagta tttgtgatga atcactaaac
     4801 tttccaaact catttgaggg atattgctta aagattgaac agatcaattc gaagccctat
     4861 tagtattcgc tcaatggggg gatcagaggc cataaaaaga gactagtcat tactagtctc
     4921 aggtagtgga attgtaggat tttaaacgtt cctggagagt accggtatgt aactcgaaga
     4981 ggtgattatc atagtcgtaa aagtaaattg aagaagcctc gcctttcaca cgagaacgtc
     5041 ctggttcgac agttaaacct aacatttgga tttttgagac aaacgaatct atatcttgtt
     5101 cgtcaatttt aaaagcaata tgattgtatg tctttggcag tttattcgac gaattctgca
     5161 ttactgcaat ccataggtcc ttaactaaga aaaatttttc tggatataat gaaaattttt
     5221 ttgttgacta tcgtagattt cttttgcatc aaataaggta tttaacaa
//
LOCUS       DAZBTF010000245         4327 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.guided.141, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000245 DAZBTF010000000
VERSION     DAZBTF010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.guided.141"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            277..1755
                     /gene="msr(C)"
                     /locus_tag="ACHGNS_003098"
     CDS             277..1755
                     /gene="msr(C)"
                     /locus_tag="ACHGNS_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFN6938937.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1802..2248
                     /locus_tag="ACHGNS_003099"
     CDS             1802..2248
                     /locus_tag="ACHGNS_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFN6938938.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2447..2962)
                     /locus_tag="ACHGNS_003100"
     CDS             complement(2447..2962)
                     /locus_tag="ACHGNS_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFN6938939.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            3079..4281
                     /locus_tag="ACHGNS_003101"
     CDS             3079..4281
                     /locus_tag="ACHGNS_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN6938940.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1301 a          727 c          935 g         1364 t
ORIGIN      
        1 aaaatgactg catcgatgaa attacgtttc gaacttttga ataacaacta aataaattca
       61 aaagacatgt ataaacaaca atttcatgga tggagtatgt ccattttttc gaacgacagt
      121 agaataggtg aaagctatga cagatttggt gatttagtca tagttatttc gttcagagta
      181 ggaaaagatg tgatgcagtt tttttgcaaa cagatttttc tgctcttttt tagtatcgat
      241 aattgttgtt ttacatgaaa taggagtgta aaacatatgg aaaatttagc agtaaatata
      301 acaaatctac aagtcagctt tggcaaccag ctagaactat ctattgattc tcttcgtgtc
      361 tatcagcaag atcgtatagg gatcatagga gaaaatggtg tcggtaaatc gactttgctc
      421 aaactaatag ccggtgaact ttttcccgat catgggaaaa tccaaacaga gatcaccttc
      481 aactacctgc ctcaattaac ctatctcgct gaagcaaagg acctaaattt ggaattagcc
      541 agtcatttcc agttaagact ggaagaaact tcagagcgga aatggagcgg aggggaagaa
      601 cgaaagatcg agttgatacg tcttctttct tcttatgaac aagggatgct tctagacgag
      661 ccaacaaccc atctagatag aaaaagtatt gatcgactga ttgaagagct tcgttattat
      721 tatggtacgc tggtttttgt tagtcatgat cgctattttc tagatgagct agcatcgaaa
      781 atctgggaag taaaagacgg agaaatccga gagttttcgg ggaattatag tgcctatctc
      841 actcaaaagg aattggagaa aaagactcag ctacgagaag cagagtcgat catgaaagag
      901 aaaaaacgat tggaaaaatc gatccaagaa aagaaaaaac aagcggaaaa gttggaaaaa
      961 gtgtccagta aaaagaaaaa gcaacaaatc agactggatc ggttgtcttc ctctaaacaa
     1021 aaagacagtg tacaaaaagc catccaaaag aatgcgaaaa cattagagag aagactccaa
     1081 aaaataggag aaacaaccaa gccgcagcag atgaaacaaa tccgttttcc agtaccaaaa
     1141 tctcttgaac tccacagccg ctatcccatc atgggacaaa atgtccaatt ggaaagaagt
     1201 gggagaacat tactggtaaa tggtgatttt cagttttctt taggtaaaaa aatcgcgatt
     1261 gtcggtgaaa atggttcagg taagacaacc ttattagaac atatccgcaa acaaggagaa
     1321 ggaatccttc tctctccgaa agtaagcttt caagtatatc agcaaaaggg ttatcaaatg
     1381 acatctgaag aatccatcat tcgttttgtc atgagacaaa cagagttttc agaatcgctt
     1441 gtccgtagtt tgctgaatca cttagggttt gctcaggaaa ctttgacgaa accgttatgt
     1501 acattaagtg ggggagaagc gacccgtctg acgattgctt tgctttttac taagccaagt
     1561 aatgtgttgc tgttagatga accgactaat tttattgata tggcaacgat cgaagcttta
     1621 gagaagctaa tgcaaatata tccgggaacg attttgttta cttctcatga ttcttacttt
     1681 gtcgagcgta cggctgatga agtttatgaa ataaaagggc agaaaataaa aaaagtactt
     1741 acgagaaatt tttaaatgtg ctacgatgat tttcgtgaca aatttaaagg agtggtacag
     1801 attgcctaca aataaaaaag aaggaataat ttttacaaca atgatgtgtt ttttgatggt
     1861 cttggggatg agcgcgtata atttatggct gcatcaagat cttcatttat caagtttgtt
     1921 gattggtcta gtgcctggat tcgtggtggc tttcattttg gatgtcttca tcatcggtgt
     1981 gctggcgaaa aaaattgcct ttaaactacc agtaaatcca gaaagcaagg tccaaatgat
     2041 cttaactatt tcatgcttga tggttttagg aatggtgaca tgtatgtctt tattcggtgt
     2101 gatcatggaa caaggaatac ctgatcaatt attctctgct tatatgaatg cttggaaaat
     2161 gaatgtgatt gccgctttac cgttgcagtt gatcatcgtt ggcccgcttt caagaagtgt
     2221 gctaaaagca attcaaagta atgaatgatg gaacactgtt atttgaaaaa aggccttggc
     2281 atgctttttg tcatagtctc ttgaatgcag ttctagaaaa atagtgatcc gaacgatagg
     2341 ttttggcctg tcgttcggat cgctttttct tttccaactg tattgctctt ttattgaaga
     2401 taatttgctc ttcaatagat atttctagaa aggttctgtt tatcccttac tcaaaaaact
     2461 gctgcatttg ttctcttact tgctgacacc attggagttc ctgctgacaa agcataattc
     2521 tgcgctggat cacaacataa cgtccaaatt cttgtttctt gtctagcgag aggtcattca
     2581 attttttttg atttttttgc atgattttgt tcagctgttg ttctctagtg aatagcagtc
     2641 cagtagctgt tgttttatcg agcgtcgaaa aagcatagat ttttgctaga aattcatctt
     2701 tttgcgtagg agcgggagtt tcctctttga tccattcctc tacaagagca cgtccttcat
     2761 cagtcaagtg gtagatcttt ttttgagagt gctcgtcttt gtccaaaacg gtcacataac
     2821 cctgctggtc taacttggca agcgttgtat agatctgact gtgatgggct tcccaaaaaa
     2881 ttgtgatcag ctgcttcaat tcatacccag aacgtggttt ggataaaagc acgcataata
     2941 atatataggc taaagtattc ataaaaatcc tacctctcgt cttattttct ttattatatc
     3001 acagtaaagt ttccacgttt tacatgtgca tattgacatg taaaataaat catgtatgat
     3061 gctaatagag agaaagggat ggaaatattg gagaatcaaa tagatataca agaacaaaca
     3121 aatgaagaaa caaaggggaa aagttcagcg ctagttgtag cagctacctg tatgatcgct
     3181 tttatgggag tgggtctagt agacccaatc ttaaaaacga ttgctgcaca gttaaacgct
     3241 acgccagctg aaacgaccct gttatttact agttacatgt tagtcactgg tgtggtgatg
     3301 ctatttacag gtttcttatc tagtcggatc ggtttgaaaa aaacattgat gacaggattg
     3361 tttatcatcg ttgtattcgc tggtcttggc ggattatctg ggaatatcgg aatgctgatt
     3421 gggttacgtg ccggctgggg tgtcgggaat gctttatttg tctctactgc tttagcaacg
     3481 atcgtcagta ttttagttgg taacacagaa aaagcaatca tgatgtatga agcagcactt
     3541 ggctgcggaa tggctgttgg ccctctagtc ggtggggtct tgggcagtgg ttcatggcgt
     3601 tatccatttt ttggggtagc atttttgatg ttttgcggtt ttatagccct agcggtactt
     3661 ttacctgaaa ctgagaaacc aaagagaaaa gtaggccttt gggaaggtgt caaagcgtta
     3721 agcaatcata aacttcgttc agttggacta atcgcattgt tatataattt cggattcttt
     3781 actttacttg cgtatgcgcc atttttattg attggctatt ctgaattaga agtcggtttt
     3841 gtattttttg gctggggtgt gcttctggct attgcttcga ttttcgttgc accggctctt
     3901 gagcggaagt ttacgactta tcggacaatt ttagcagcat ttgtcttatt cattatttgt
     3961 ttgctgttat taggagctgg aacttctatg ccagtccttg taccgatcag cattgtgtta
     4021 gcaggatttt tccaaggaat tatcaataca ctgttgacga cgattgctat ggaaatacca
     4081 ggtctgcaac gaaacgttgc ttcgtccagc tatagttttg tccgcttttt cggtggagct
     4141 ttagcaccat ttattgcagg tgtgattggt gaacatattg gtgaaagcta tgcgttttat
     4201 tttgcaggtg ccattgttct tatcagtgta tgttgtatca tttatcaccg taattatttt
     4261 gctacaaatg aagaagaata agaaatcaga ggttgtgaaa aagctgtcct gcatcaagtc
     4321 ataagaa
//
LOCUS       DAZBTF010000246         3796 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5925
            SAMEA115708044-rid22985083.guided.155, whole genome shotgun
            sequence.
ACCESSION   DAZBTF010000246 DAZBTF010000000
VERSION     DAZBTF010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708044
            Sequence Read Archive: ERR13197769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337965.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337965.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:01:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,107
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 39 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708044-rid22985083.guided.155"
                     /isolate="VRE5925"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            110..193
                     /locus_tag="ACHGNS_003102"
     CDS             110..193
                     /locus_tag="ACHGNS_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFN6938941.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            318..1055
                     /gene="erm(B)"
                     /locus_tag="ACHGNS_003103"
     CDS             318..1055
                     /gene="erm(B)"
                     /locus_tag="ACHGNS_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFN6938942.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1318..2181)
                     /locus_tag="ACHGNS_003104"
     CDS             complement(1318..2181)
                     /locus_tag="ACHGNS_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFN6938943.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2183..2455)
                     /locus_tag="ACHGNS_003105"
     CDS             complement(2183..2455)
                     /locus_tag="ACHGNS_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFN6938944.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2472..2681)
                     /locus_tag="ACHGNS_003106"
     CDS             complement(2472..2681)
                     /locus_tag="ACHGNS_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFN6938945.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2759..3655)
                     /locus_tag="ACHGNS_003107"
     CDS             complement(2759..3655)
                     /locus_tag="ACHGNS_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN6938946.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
BASE COUNT         1174 a          662 c          586 g         1374 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccaaa attttgtata ataggaattg
       61 aagttaaatt agatgctaaa aatttgtaat taagaaggag ggattcgtca tgttggtatt
      121 ccaaatgcgt aatgtagata aaacatctac cgttttgaaa cagactaaaa acagtgatta
      181 cgcagataaa taaatacgtt agattaattc ctaccagtga ctaatcttat gactttttaa
      241 acagataact aaaattacaa acaaatcgtt taacttctgt atttatttat agatgtaatc
      301 acttcaggag tgattacatg aacaaaaata taaaatattc tcaaaacttt ttaacgagtg
      361 aaaaagtact caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg
      421 aaattggaac aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa
      481 cgtctattga attagacagt catctattca acttatcgtc agaaaaatta aaactgaaca
      541 ctcgtgtcac tttaattcac caagatattc tacagtttca attccctaac aaacagaggt
      601 ataaaattgt tgggaatatt ccttaccatt taagcacaca aattattaaa aaagtggttt
      661 ttgaaagcca tgcgtctgac atctatctga ttgttgaaga aggattctac aagcgtacct
      721 tggatattca ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc
      781 ttaagctgcc agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac
      841 ttacccgcca taccacagat gttccagata aatattggaa gctatatacg tactttgttt
      901 caaaatgggt caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa
      961 tgaaacacgc caaagtaaac aatttaagta ccgttactta tgagcaagta ttgtctattt
     1021 ttaatagtta tctattattt aacgggagga aataattcta tgagtcgctt ttgtaaattt
     1081 ggaaagttac acgttactaa agggaatgta gataaattat taggtatagg gcacctctaa
     1141 taactaccaa ttaattcacc tcaaaatgaa attagaaaaa aacagcgaaa tttttaaatc
     1201 tatttcttat cgatacaaat tcctcgtagg cgctcgggac cccaacatga ggaaatgttt
     1261 ttataaaaaa agagtagtta ccgttttttg gtaactactc ttttcctttt ttaaggttta
     1321 aatacctgga agtttaggtg ttttgggtat tggtggtgta ggtagctgca agctttccat
     1381 tttttgttga attgctttaa attcaggggt ttcttggtgt tgattttgaa ccattttttg
     1441 ctctattctc tccaaagtcg gttgaatttc tttccctgat actttacgat ttaattctct
     1501 ttctaaggtc tctttcggac taatggaagg cgtttctaag cttgaataga gtttcactcc
     1561 ttctctattg taaagcctta tatcgctaaa taagcccgtt ttatgaaggg tttctaaatt
     1621 ggtcggtaag tttttgacaa caatatcatg cgcttgtttt ggtgttgccc tagctgtcat
     1681 tggatcatct gcatacatgg tttcatatcg ttcaatcgtt cctaagtatg attcaatttt
     1741 cggtactgcc atgacgtata tttttgtttc ataaccttta gattgaagca ttgtggctgt
     1801 ttgaatggga acgtctgttg ttcgtcctgt tccttcaatc actaaattat agccttgatc
     1861 gcttaaacgg ctaataagtg cttctgtcat gcgattcgaa taaggggtaa cgtgttttac
     1921 tacgtctttt tcatagagtt tcactaattc gtcaaaattt gggtgctgtt gtttaaaggt
     1981 atcattatca atgacaataa cattcccttg tgtttcttca aaaattgctg atcgcaaact
     2041 ggttttccct gaccctggtt gcccaccaag taaaaaagcg gttggcgatt caaccgctag
     2101 tctattttta gttagtcgtt caacattctt ttctaaacga tcttcaaatt gcttttcggt
     2161 gaagtcagta atattcgcca tgttaagcca ctttctcttt attcaaaatt tgttttgaat
     2221 attgattcat tgaccgccaa tactcatgaa tagcttgtaa ttcttcaaat ggttgttgaa
     2281 ataaatcaac ttcttgtgcc acttctaatt gatttaaaag attcacttct agtaaacgag
     2341 tatttttagt atctagttca tgattcaaaa cataatttaa tacccgatta taaacacttg
     2401 ccgataactc attaatgatt tcaatttcaa atgttttttc atacgtaact gccatttcat
     2461 tttcactcct tttaaagttt gtcaggtaaa tatttccgta tatattcttc taaggcttga
     2521 tccataactt cttttacgtt tccgccattc tttgctgttt cgatttttat gatatggtgc
     2581 aagtcggcac gaacacgaac cgtcttatcc cccattatat ctttttttgc gctgattggc
     2641 gtatcatttc gttttgcttt ctgtgcgcct aaatttccca cgatcgctca cttctttcta
     2701 tttcttctta ctatttattt tatcatcacc tatcacaaat cacaagtgat ttgtgattct
     2761 attctttttc attttctaat tgaatgattc gctcaatcat ttcaaaaaat acgttctcat
     2821 acatagacaa aacttttttg tcatagcctt tgtgttctgt aatgccattt ttagaccaag
     2881 tacttacttt attacttcgc ttgataatat ttcggaaaac caaattatcc tctttatgtt
     2941 ctttgtacag ttcttccagg tttgatttta tcgttgcgct gtccgtatca actaaataag
     3001 gaacaaaacc aatcatatct agtccagggt taaactgttc ttgcaaatca atcaaatagg
     3061 aaatatagtt ttgaatgttg tttgtacttt cttcttctgc ttgtaaaggg atcataacgt
     3121 aatcactcgc cacgattgca ttatttgtat aaacgcttgg cgttggtaca gtatcaataa
     3181 taataagatc atagtcactt tttaaaggtg ctaaaagagt agcaagcaat ctactttcat
     3241 tttcaaacgt ccatgagcga gttaattttg gcagtaacat caaatcaaac gtgccaggga
     3301 tcaagtctaa attatcagtc aaatgaataa tagaagaagc caagtttcca tttttcaagc
     3361 cttcataaaa attgacacgt ggcaattcta cctcaaaagt ttttgctaag tcttttgtca
     3421 atgttgcctg taagtcctta tcgatcatta aaacttttaa attcaatttg tctgtcaagt
     3481 aagcaaacat agtcgataat ttggactttc caacaccacc tttaaagtaa ttattcaaga
     3541 taataattgc ttcattttta ttgtttaaaa tacgtcttaa ttcttcaagt atttttagtt
     3601 tttccttctc cataactacg ccccattctt tttttgtata gtaatattgt atcattaaga
     3661 atcacaaatc acaagtgatt tgtgattaat cacttgtgat ttgtgataag tgatttgtga
     3721 ttaatatata aaagccctct ttaaagggct tttataggtt ctgttgcaaa gttttaaata
     3781 aagaataaaa tccctt
//
